LOCUS       DAOQYI010000001       231237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_29_length_231237_cov_75.641360, whole genome shotgun sequence.
ACCESSION   DAOQYI010000001 DAOQYI010000000
VERSION     DAOQYI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 231237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 231237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231237
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29_length_231237_cov_75.641360"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1104)
                     /locus_tag="PKL93_00005"
     CDS             complement(97..1104)
                     /locus_tag="PKL93_00005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNQ69683.1"
                     /translation="MSFTVDFYEESRKNVRKTIVLVMVFLAMMIAFGLIIDLIFGILP
                     VFTFVFLAIALVQTLISLSSGKDIVLKSVKAREARTDDPEERQLRNIVDELSLAAGMA
                     KPPEIYVIDDDSVINAFATGRKQENSVVCVTTGLLKSLDREETSGVIGHELSHIVNRD
                     ILLMTLISALLGAVVIVQSLAFRALIAHLRFGAISATSRTKRSSKKNDNSALAIIAFL
                     AAVAGLGALFSFIGRLSLLAVSRTREYFADARAVELTRNPLGLSRALRKIVTNSAKLQ
                     TANVATAHLFISDPLKRRINNKSSFFASLWSTHPPIAMRISILENRSLKEIEDELSDY
                     I"
     gene            complement(1110..1673)
                     /locus_tag="PKL93_00010"
     CDS             complement(1110..1673)
                     /locus_tag="PKL93_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ69684.1"
                     /translation="MVILFIVIGIIVVLALWLIGVYNNLVTLKKRVENAWSQIDVQLK
                     RRHDLIPNLVNSVKGYMKFEQETLDKVIQARAKAVSAQGVGDRIKAEQELGGVLGRLL
                     AIAENYPDLKANANVSQLMEELTATENKISYARQFFNDSATKFNTKIEIFPNNLIVGF
                     FGGKFEQFPLFEVTEAERETPNVDINF"
     gene            complement(1698..2486)
                     /locus_tag="PKL93_00015"
     CDS             complement(1698..2486)
                     /locus_tag="PKL93_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNQ69685.1"
                     /translation="MLTNKRLFVSDMDGTFYLGDGLLKGSLEFANAVHRLGSKLVFLT
                     NNSSRTPEEYIRKLTKMGVDRTLFEVYTSGEATISFLKRDFPNKRVFLLSTPSVREMF
                     RRSGVNLDDNSPELLVLAYDTTINYERIRKAALFIRQGIPFIATHPDVNCPTEDGPVP
                     DVGSFLSLFEASTGRRPDNIIGKPDPTILKMVMDDFGYGPEETVMIGDRIYTDIECGL
                     RAGVDTYLVLSGETTADMVPDSHSYEVVNNIGAIIEIIERGNTV"
     gene            2548..4149
                     /locus_tag="PKL93_00020"
     CDS             2548..4149
                     /locus_tag="PKL93_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNQ69686.1"
                     /translation="MKRKFERKYFFIIFMIVSGFFIFIALGNGFWRESAPYKIDSVEI
                     NAKLDEQGDLHVEELSVYILSRNNRTASKKLHLSYPSVLKSYSVEIPNDAGFVKEISS
                     SPGGFDARIFLEQEVSEFVMNTEYVISGVVEVGSDIAVLSYRFWEPNSDAMTRDISLA
                     MELPETIISRLTEDDISVRPFKEASFEIEGNLVKLQMKSVLSNASGEIKISFPKSIAN
                     TMPNVVNTTISKSALRKEHESAMFQQAFLSGLIGFQIAVPFFVIFFTFILFGIEPQVK
                     SEIGYRLYDVPGYLLNAVIKNPFQEIDHNGFLSTIMEMHNSGEIQIGENTISFETSEK
                     GIPTQQKWALQTLKSLRRNEDGSIEIPDAESDAFSFDEFKEQYGFWRKHAMRVVKSGR
                     FYEYLGSTVMSVFSILYLIIWSLILKFVFGNWQVYLSFPDESLAISIVLYVDWCLGWL
                     LLVLPKNIFARWTPSGRLFYMEWKKTEKELLSKQSWTNDDIAKLVALGHLQDLISNRK
                     RVSDQQLTLLRQLQRLEMIFNKVRP"
     gene            4255..4506
                     /locus_tag="PKL93_00025"
     CDS             4255..4506
                     /locus_tag="PKL93_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNQ69687.1"
                     /translation="MKNQMNAKVIVYSTPSCPWCKRAKDYFRTNGIPFKDYDVSKDRA
                     KAEEMVKKSGQMGVPVITIGSQVIVGFDKNRIDNLLGIH"
     gene            4618..5721
                     /gene="galK"
                     /locus_tag="PKL93_00030"
     CDS             4618..5721
                     /gene="galK"
                     /locus_tag="PKL93_00030"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNQ69688.1"
                     /translation="MLKRYFVAPGRVNVIGEHTDYNQGFVMPVAIDKYVLLSIERTDE
                     SVIALSSAGREPVSFREESIEKTGDWSDYLKGVFWVLKDNLGVDFGGMKIKISSSIPE
                     GAGLSSSAALEVALILALNSVYGLKLGDKELYSFAQQAENEFVGVQCGIMDQFAAVMG
                     KKDTAIFLDTLEMRHEYVPLELGEYTFVVFDSKVHHSLSSGNYNTRRAEANKALEILG
                     KNSYREVSMIDLFPNRGKLGDLYYRRALHVVSENMRVLEAMKMMSNSNFENLGRILIQ
                     SHESLALDYEVTCEETDFIVDTLREIGGVSGARMIGAGFGGSVLALCEKIEMKKIVEV
                     MKIRYKERFGIDLDSYEVRPSDGAREVDATFPL"
     gene            5795..6796
                     /locus_tag="PKL93_00035"
     CDS             5795..6796
                     /locus_tag="PKL93_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ69689.1"
                     /translation="MKVNLGIVGAGIASRELHLPALRRLTELFKITAVNSRTRRKAEE
                     FAKIAGGDVEVFDSYEEMLNSDVVDAVVLAVPIALNPQMIEAARKANKPVICEKPVAA
                     TTKEAIRLLHLPGNTPIYIAENYRHIEVYRKAEILIKEGRLGKPLAFSWLKWVDFGQD
                     NKYVQTKWRQTPQHIGGFISDGGVHDVAALRKILGNVEEVSGFSEKNFDYLGAENSAV
                     FNMTLGSNVIGNYSVVYGAPASLNRFEIVCTEGIVQVDKDEATLEIYSSDREKLFLNK
                     TDGFAEEFTDFHRVLEGQINSLGSVEEAVMDLATIEAGLISAKEKRVVRVDSLLNES"
     gene            6804..7946
                     /locus_tag="PKL93_00040"
     CDS             6804..7946
                     /locus_tag="PKL93_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ69690.1"
                     /translation="MRGFNTRAIHAGEEKKINDAVSTPIFQTSNFLADDVKYMDESSE
                     VLYTRVGNPSIGVVERKLSDLFGGTGGVFFSSGMGAITTVFLSILKSGMNLVISRNIY
                     GGTQSLISDLPGMGVEIRRFDQSRIDELETLIDDKTGIVYVESMSNPDLILSDIEGIA
                     RITREKEAVLVVDNTFLSPYNFRPLEYGADVDIQSLSKYVNGHSDVIAGFAAFKDSGL
                     EKRVRKVMIKLGTNGAPFDAFLVGRGAKTLGPRMELHNRNAREVAEFLSKNPEVLRVS
                     YPSLKASVPACFSECRGFGGVVYLELESLSAAKSFIRRSELFLEATSLAGVESLATIP
                     VLTSHASLSSEQLRDAGLSEGGVRLSVGIEDLEDLLSDLDFAMKGI"
     gene            8144..9916
                     /locus_tag="PKL93_00045"
     CDS             8144..9916
                     /locus_tag="PKL93_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNQ69691.1"
                     /translation="MLKIRVSSIEEFLLADRKEFKMEAGNFVYIYDFERLTDSPGILL
                     QISVVNRSDAPKKVGKIDLLEIEDVSEPVYYNGWQSWLPFKAYWEQPIVDPFVQYADS
                     HEISLFSATPIPEILREGRRPSDYFIATDELVVGFLSSRFSHQYFLWNDDQCNIRGSA
                     DLFGAELAPGESVQLEKMVIFGKDSLWSMLENYASLIKKSNKVEFNDFSGVGWCSWYQ
                     YFSDITFEQLSKNIRLLAEIRDREKIDYRLVQLDDGYQEDIGDWLETNSKFPELKEIA
                     SEIRKRKFSAGLWIAPFSASETSKVFREHQDWFVRNEDGSPRIAYRNWGKKIFSLDTT
                     NPEVLEHLRDLIVSIRRSGFDYLKIDFLFAGAIPGRRYREDCGPIEAYRIGMKAIRDA
                     AGKDCFILGCGAPLLPSVGFVDGMRIGSDTSPQWNGEAMDIGIPSARFSLRNAYTRAF
                     MHRRLWLNDPDTVILRKCEMTEEEKRIFALSVGLLDGMMINSDDMELVDSQGISLLKE
                     AIMLKGGEPRVFLDGMNSVFATCTRKANYCNVTSFVNLTDTPRHSIKLKEMYENWSGV
                     KPPVKEVELPPKSIWIAKSDSCDI"
     gene            complement(9970..11994)
                     /locus_tag="PKL93_00050"
     CDS             complement(9970..11994)
                     /locus_tag="PKL93_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNQ69692.1"
                     /translation="MMLVKKMMIILFLTVMTLGCGVSFLTTSESTPSVVVMEKSMTLE
                     MMRLENDWWTAEVSLEPGRYRYYFTTEESSFPDERNDEKSYENEREMSVMIVEDFEVL
                     HDSVRHSVGDRDYLNPINHDEYYFAVSAPTDVPFEAKIVIAGERYPLEYLRDFGNQSF
                     YRTERLSVEELEYYFEIQTDSGVVFLDSLGLCRKPRIPYCFSKESLPISYFDLPDWSK
                     GATYYQIFPERFANGDPRNDPPNVQEWNADPDYANLGGDGFFGGDLKGILDHFDHLLE
                     LGVDAIYLNPIFESPSSHKYDTEDYLKIDDNFGDDQLFEELLTAANDSDVKIILDGVF
                     NHTGYDFFAFKDLRERGEESPYVDWYFVKRFPIRKVQDRAMTYVGWNGYAYMPKINAL
                     NKDWQSYVERLVRKFDCDGIGGWRLDVATEVDPLFWTDFFRPLLKSLDDEMILVAEFW
                     GDARSLLRGKSFDSVMNYPFKDAVVEYVFRAGNSAAKFAAMTNLYLNAYPPQTLHSLW
                     NILDSHDTERILTLAFNQIDLMKIAVALQMTFIGSPVIYYGDEIGMKGGRDPENRAPM
                     IWDEERWNHDVHDFYKALLDLRRSHESIRTGEYEVIAAEGPVFSFRRWKATDEVIVIT
                     NPSREAAFFSPSMEGEFTEYLTGETISFFGGEIKVPPLTVWILTPRDSVN"
     gene            complement(11991..12920)
                     /locus_tag="PKL93_00055"
     CDS             complement(11991..12920)
                     /locus_tag="PKL93_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ69693.1"
                     /translation="MAEKERDRLLAYFAGIGSSVIFGLSFLFTKNALEYTNPMYFLSY
                     RFSVAFVTISLLRALGVIKVRPYKSIFKDLLLLSLVQPVMYFIFENVGVSLSTSSEAG
                     ILLSTIPIFVLIFARVFLKERLAAPQVFSVLTALLGIVLITAGKGLSTGGSVLGPIIL
                     LGSTVSAAFYNILSRKASVRFKAWEITYHMMTMGFISFTLIAVVTSLIKGEFSFYIFG
                     LGTPQVAIAALYLGTLSSVGAFFLINFMLSKLEASRSTVFVYLSTVVSLLAGVVFRGE
                     RFGPLQVIGISLILLGVWGANALARRKTREESK"
     gene            13042..15510
                     /locus_tag="PKL93_00060"
     CDS             13042..15510
                     /locus_tag="PKL93_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69694.1"
                     /translation="MFRRYFLITFLLLSAFLSGYSFRDLDWLKHESDHFVYLYHKEVA
                     SSIFQVEALAEDAFDTLSELFGNPFKEKITVIIGGYEDRSNGLANPVLESIYIMTVGM
                     DYPYRNDGFWLEEVITHELTHLFQITSVTEFGNILRNYVSKAYLPNALQPMWLTEGFA
                     QLGSELIGDLYEYDYRRLPFLLDQLMENEPFSEETVVTGFSGIGGESYYNYGYAFLSY
                     LSETYGIESLLELVKVKRGLLGLTGIELAIKKVYGKSYEELKEEFIEYQIKKWEAAEV
                     PFVYEFSRGVGEFVNHFRPKIYDGKLYYLSLDRQMKCYSLNIEGVEIFNSTMEIVDFA
                     VFESEIALILFEEEQSETRLYFFREGKLERTKTAHLREIDYLGKDRLLVVRNNLGISS
                     IEILSLRTERVTRIFTPLDPSTQIDNIRVSCDGSLIAFRINNKGDKSLAIYNSSTEKF
                     GFFDVTNDFSIGGWVSEGFLISVKNEFGSDVYVVTSEGEMIGKSSFSRYVREPVMIAD
                     GVIAVGQLQGRKLLFSSNTKPTDYSLESKEIILSPPQEIEGVAYNGFSDWRFVSLLPF
                     KGISVLFSDLTFRNKLLGGVSFDFETMLPGINVQLFSEDYLPVDFSLSLEYANLEPKA
                     LLNVYRRYRIDPKLSFGWKASLEYPLRIAGSLDAALEDSSALYGGQASLNTLINVRDT
                     QTATSNLVDVGINVDFDWNKHGFTIDSTIYSRVTFGEEPSIVPVELWGFRDDTNIVVG
                     FSLDSDYVISRRGLNVMNLVHFSEEGIGGKIDFLIGSRFSWRIAIYKFETAFLYAAYP
                     MHIKAGIMLENTRLLPYLDFELIY"
     gene            complement(15499..17748)
                     /locus_tag="PKL93_00065"
     CDS             complement(15499..17748)
                     /locus_tag="PKL93_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNQ69695.1"
                     /translation="MKKRFFRYLNSVVKVSVVREGKTKLKSGAVIIEPEEELDELGAC
                     QLLGVLDLGQARTFVSRAGNEMSVAENDAGYVVNITLQKNQAVLGLGETMGPLNKRGS
                     IYEMYNTDEPDHSPSKRRLYSSLPVFHIVSPEEQFALFLDHPGYSSFDIGFENRDTIK
                     IGIEGDGFDLFIFVGSPSDTIRRIFKLTGNPLLFPAWSLGYHQSRWSYANEEAVLEIA
                     ENFRRRRIPCDAIHLDIDYMDDFKVFTWDKNRFPNPSSLMANLSSMGIKTVAIIDPGV
                     KAQDGFDIYEEGQKKDLFCLRKDGSPFRAAVWPGESRLPDFLNSAAREWWGQHYDRLI
                     KNGVSGFWNDMNEPAIFYTPESLLELKLMAEELHDSGIETEFLFGKIISKKKYYDHGV
                     DFVQKDDDGNTHSHREVRNIYGFNMARSAYEGIRKSKSDLRPFNITRSSYPGIQRYAV
                     LWTGDNASQWEQLLNEIRLIQSISLAGVSFTGCDVGGFGDDCSGELLVRWTQFGAFLP
                     FFRNHSAIGTRNQEPWAFDKEVEELVREAIELRYSLLPYLYSVHKQSANGEMTMVRPL
                     ALIWPGDSETYYADDQFMIGPSIMVAPVYQRNSEGRHVYLPGEEWLDLNTRKVVNSGH
                     SWAKAPLNIIPYFLKKDSLVAMTDGLQFVKSGSWKTLKFLGFVKDRAEFYLYEDDGLT
                     EAYKKGEYSSKTIIVDNTSNGLKVEVKPQEGSFKCDERRIEFEIHDGRKLYKRSIIDS
                     PDGCEFLIY"
     gene            17839..18153
                     /locus_tag="PKL93_00070"
     CDS             17839..18153
                     /locus_tag="PKL93_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNQ69696.1"
                     /translation="MTVREAVCKIKSLRAERLSEISGTYMFFIAGGEPSKFAVEILDG
                     RLEVYEEDKPSDCSISTDLETFEGIVGGAVNPFAAFMSGKLKISGDLTFAMKLNKLLG
                     GE"
     gene            18154..18504
                     /locus_tag="PKL93_00075"
     CDS             18154..18504
                     /locus_tag="PKL93_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69697.1"
                     /translation="MSAEVIILAFEGKKACFLHALLNALDMSEKGIEVSIVMEGESPS
                     LLKEIVNETDALHGLYSEAKRKGLIKAVCYGCSKLMGVLEIVEKEGLPLNGSMKNHVP
                     LAPYVERGAEIITF"
     gene            18583..19224
                     /locus_tag="PKL93_00080"
     CDS             18583..19224
                     /locus_tag="PKL93_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNQ69698.1"
                     /translation="MRNSLRRWKNEKLSERLAKVFESKAIKFMYFPDKEMVVDAVSKL
                     VPDEATVSAGGSLTLQETGVSDLLKSGKYNFLDRDSVSGNEEKKRIMISAFEADYYFC
                     SANAITESGEILLLDGNGNRAAAVTFGPDKVILIAGVNKVVSDLDAGIKRIRSIAPMN
                     AKRLNLHTPCAESGLCTDCDSDERICEIYSIIVDSRRRPWRYTVILVGEELGL"
     gene            19221..19487
                     /locus_tag="PKL93_00085"
     CDS             19221..19487
                     /locus_tag="PKL93_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNQ69699.1"
                     /translation="MRTVRIRVYGRVQGVGFRYYALYTARSLGITGYVRNEPDGSVEI
                     VGSGRDPDIEAFIESIDRGPTYANVTKTEIEELPFMRFEYFEIR"
     gene            complement(19494..20153)
                     /locus_tag="PKL93_00090"
     CDS             complement(19494..20153)
                     /locus_tag="PKL93_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ69700.1"
                     /translation="MDFDRLINCIPHIKEILGVVEKAFENYDDPAHDISHVLRVTESA
                     SEIAIREGANLEIAVIAALLHDIKRPEEDFTGIDHAESGAKYAFQLLNEMGFESQFTF
                     EVSEAIKVHRYSSGLNPSSMSARILQDADRLDAIGAIAVARVFSFSGKTGTPLHSPKL
                     KPGDSYKGYSSTAINHFFEKILKITPESFWTKTAKEIAENRYRFVVQFVKQFLSEWGG
                     I"
     gene            20250..21008
                     /gene="surE"
                     /locus_tag="PKL93_00095"
     CDS             20250..21008
                     /gene="surE"
                     /locus_tag="PKL93_00095"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNQ69701.1"
                     /translation="MNILVTNDDGIMSPGIIKLAEALSEEHDVLVVAPDVERSATGHA
                     ITIRTPLWAKQVKVGNKNIGYAINGTPADCVKLGLLAISEKKIDLVISGVNKGQNMGI
                     DVLYSGTVSGALEGAVMDTPSIAVSSSDWSNPEFETAAAFMVSFLRVFDVKMMPDFTA
                     LNINVPSVKSEELKGWKVTRQSRRRYKDYFEKRKDPYGNNYYWIFGEVVEDECSTDCD
                     YNAVKSGFVSITPLHAIMTDNDYFEKLKEISRGW"
     gene            21011..21502
                     /gene="def"
                     /locus_tag="PKL93_00100"
     CDS             21011..21502
                     /gene="def"
                     /locus_tag="PKL93_00100"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNQ69702.1"
                     /translation="MKVIYIGNPILRDVSEKVEVFDDELRQFVNELSKTMYVEDGVGL
                     AAPQVAISRRLFVYDPGDGLRVVVNPEILFRSDEKVKIEEGCLSIPGIYADVVRPASV
                     RIRYQDEYGHYHEDDLTDYPARIVQHETDHLDGILFVDYLSSAKRAILKPKLDQIIKE
                     SVG"
     gene            21503..22435
                     /gene="fmt"
                     /locus_tag="PKL93_00105"
     CDS             21503..22435
                     /gene="fmt"
                     /locus_tag="PKL93_00105"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNQ69703.1"
                     /translation="MNIVFMGTPEFAASHLKALVESGVKVIGVFSQPDRPKGRGRRVY
                     PTPVKSVAEVYGLPVFQPEKVNSGEGLEKLKELSPDLIVVVAYGKLLKSSVIDLPTLG
                     CFNVHASLLPKYRGAAPIQRAIENGETRTGITIFKIDEGMDTGEIALRREIEIEISDN
                     FGSLYEKLERLGREALLDFLKIAEAGRIELCKQEGAPSYAPKVQASDLVISDFSDAVY
                     VLNKVRAYDPQPGARTSLDGKMVKLFGAVFSDRSYGKSRDGEIVKVEDHGMVLSCGTG
                     NVIISLVQLPGKRPMSPKDAVSGRLIREGMILGG"
     gene            22438..22653
                     /locus_tag="PKL93_00110"
     CDS             22438..22653
                     /locus_tag="PKL93_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNQ69704.1"
                     /translation="MPRVIRLFQIRNEITDSDAERIVSRLKSLDGITKAQVDVASSVV
                     EVEYENAIIDRYIIQEELSKLGFDMLI"
     gene            22721..22809
                     /locus_tag="PKL93_00115"
     tRNA            22721..22809
                     /locus_tag="PKL93_00115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22755..22757,aa:Leu,seq:gag)
     gene            22852..25404
                     /locus_tag="PKL93_00120"
     CDS             22852..25404
                     /locus_tag="PKL93_00120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNQ69705.1"
                     /translation="MGEILLGKVFKRNNEIKIGLRRLFESASFVSINIKDLPSFFSER
                     DEDCLFIDASVEEVYLSEIAVDERFLNSFVYQPESIDLESRIDNYIRVIKKLPIGLSS
                     LIEKIVPDRGLRNFLLFVLKQSENVDPWEDFLLSGLTRSERRESGNSRIDIKKAVRLV
                     FSEGGLLQDSMEGYEFRQEQFMTALEIAESVEKGGNLMIEVGTGTGKSIAYLAPVAYA
                     CVSKMHQAVISTRTRILQDQLFKKDVEVLKTLPNLDNLRVSVIKGRERYLCVRKLFEV
                     LNMALNKMLDESSSKELAGVILWSMFTREGDLDYLPLGDEFRRMLSGNRFDCTRRLCP
                     FFEVCPYYVQRENAKNSDIVITNHSFLFSEANIRLSDGEPEDGESIGTLLPAFKYLVV
                     DEAHELESSLTQAMSYRFEPYQLANTVKKLIKLVRDCFGFLKRHFSEDFLRKLWIKLK
                     EATTTLDRKIRQLVVDLDRERNGEKTTYSGKGLETLVSALTVISDILQTHIYVGGQTV
                     QMLDDVEERDLKIDGLEFEVARVLAEIKEWLKQIAGIAASRDSRVVFVSRNEQCIESL
                     SIVSSPVENDTLMSSIFPDVGVKIFISATLWVYSRGSDGFNYARRILGAGEETDAVRL
                     GTSFDYTQQLRFFVPTDIPDYLPNSLPYMQSASKLTKDVLKIVGGGSMVLFTSYEDMK
                     WTLSKIRGELHNLSLRIQERDDSLTTILSEHRNSEKSVIFGARAFWEGIDLPGEQLKL
                     LIVYKLPFERPDEPLIEARIKHYGKRSFAEGMNKYYYPKMITAFRQGLGRLIRTRNDR
                     GVVIVLDRRIIDPARVYSKKLIASLPPGTNIQAINSKQILRELRRLKKERWL"
     gene            25404..26033
                     /locus_tag="PKL93_00125"
     CDS             25404..26033
                     /locus_tag="PKL93_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNQ69706.1"
                     /translation="MMKKLISHFFPNHCLVCEKEIDPAQYLCADCLGSIKGPIMTPFE
                     LKFVDSAFSYWKYESPLKELIRAYKFEDRPGIASLLSEMLFEMINTFSPSADVIVPVP
                     TSIQAFVKRGFDTNLRILRRLSKTIDIRIDNVLTIAGKSIPQSRLKMEDRFQNVKDKF
                     YLKRSPRSTSVILFDDVVTTGATASQCARVLKEGGVKEITLLAIAGGKK"
     gene            complement(26049..26123)
                     /locus_tag="PKL93_00130"
     tRNA            complement(26049..26123)
                     /locus_tag="PKL93_00130"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26088..26090),aa:Gln,seq:ctg)
     gene            26182..27339
                     /locus_tag="PKL93_00135"
     CDS             26182..27339
                     /locus_tag="PKL93_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ69707.1"
                     /translation="MNIAMFSDTYSPQVNGVVTMIKMLEENLQKRGHNVYIFTVDHPE
                     AGIQENVYRIPSLKFPWEKQHRIGLPTNFKELIKIVKNLEIDIIHSHTSLIVGYLASY
                     VLTNLHIPGVTTYHTMMEEYVHYIPFMEPILRVYIRAQDRRFCDKHRAVIAPSIKIKK
                     LLLSYGVSSHIEVIPNGVDLTPFKRSFSREEKRAFRKKYKIKEDDKVLIFVGRLGEEK
                     SIDKLIENFARVSEALPDSHLLLVGDGPLKGKLQELARSLRVGEKVHFTGFLRWPDEI
                     SLAYKSSDAFMIASHTETFGLVTLEAMASGLPVVAYKDDSIVNMVLDGENGFMCSSKD
                     ELSNAAIQMLSDHSLRERMAKRSVEISEDFSAEANVERTVNLYERVVKNEI"
     gene            complement(27323..28333)
                     /locus_tag="PKL93_00140"
     CDS             complement(27323..28333)
                     /locus_tag="PKL93_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNQ69708.1"
                     /translation="MKKSTRNFMIAISLSLLIILIVLFATDFSKSISYLSAANPGWIF
                     LIFCLTFGTWLFEAITLKLFAAMRNLAIPFLFLFKITVIGTFFNGITPFSSGGQPAQI
                     VFMQRRGISIGESTAMLVSRFLIYQTVITLLGAVAVFKAYPLVSQKISNLAVLSIFGF
                     ALNSVVLIGLVLFSLSPKLTDKLIHFVISFLHKIRIVKEKESLIEKAMRELRFFHSSM
                     KELVRRPFVLTSASLSTLAQLICMISIPYFVALSIGTPVNYLEITAVQLILFLVVSLI
                     PTPGASGVSEGGYVLFFKSFFGEGIGAALLIWRFFSYYANIIFGGLLSAHEIGFKSRS
                     RS"
     gene            complement(28330..29337)
                     /locus_tag="PKL93_00145"
     CDS             complement(28330..29337)
                     /locus_tag="PKL93_00145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ69709.1"
                     /translation="MRVLLYSEGKKLFSKSGVGIALKHQMSALEKVGVEYTTNEEDRF
                     DVVHINTVGPGAERILKKAKKSNIPVIYHTHTTYEDFRNSFTLSNAIAPWLKKRLIKL
                     YSSADFLISPTEYAKNLIKSYGIDLPIKAVSNGVDNTRFSKNKVLAEKFLLENNIRKP
                     LIVSVGLPFERKGVIDFCEIAEKRTDLVFYWFGAKISSLLPSKIRRLLKNPPDNVKFP
                     GFIPQEMIIGPYSACDVFLFPSYEETEGIVVLEALATEAPIVLRDIPVYSGWMKHEEN
                     CLKAKNNKEFMENIDRLLHNESLRKKLSACGKGTALEKDLSIIGQELKKIYLEVLDRK
                     K"
     gene            29821..30339
                     /locus_tag="PKL93_00150"
     CDS             29821..30339
                     /locus_tag="PKL93_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNQ69710.1"
                     /translation="MKFFITGERNVGKSYLVERIKPVVKYRGFETRFDNDLENLYMNF
                     YEGDSLLIGTRERGRLRIVEEGFLFATKLLGSLDIPNDNLLIMDEIGFLEEDSPEFQQ
                     AVVSSLRRAKHCLCVLRKGNFSFIGHLKASMGVGSIEITPENREEVYSRIVREIEKER
                     AYLPSPGGDEGI"
     gene            complement(30318..30394)
                     /locus_tag="PKL93_00155"
     tRNA            complement(30318..30394)
                     /locus_tag="PKL93_00155"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30358..30360),aa:Cys,seq:gca)
     gene            complement(30754..32040)
                     /locus_tag="PKL93_00160"
     CDS             complement(30754..32040)
                     /locus_tag="PKL93_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNQ69711.1"
                     /translation="MAKKVAILLVVSLISSAMLFSGTLSGYLTERMGWTYIASGSDVE
                     MSIYPPEGVSAIADERIFYGQFTIGDSTVNIAIMNVEPPILWIDSDYDGCFLNNIPIK
                     GQYRGTVINESRAYKFSQQLRVEYLSNGNSSYMTQDLEIHAILPKKEGVEVDYLLMSR
                     REGLLEVDGELIEIALFPTAPDGLADKIDEVFIGIDLDRDGTIDMVHKNHELFEATSV
                     IRINDKNYRVSEISRDGRKVTFEQVDEDAQNRPLLKKGSIAPLFEARDVFGETFSMSD
                     YLGNPIIVVVTSKTPLEVFNGGFPCATGDCEDTSREEKRLRSLLNELVTWNEYSAKPE
                     IELLWLYSGDEITSADSLEEYSWLHLIADKSAVELYSTPLSNGMFLISKDGRIEYLDD
                     YVLQAGDGPIPTYPVFRIPTFGLTSYIWFRELVFDK"
     gene            complement(32066..>32394)
                     /locus_tag="PKL93_00165"
     CDS             complement(32066..>32394)
                     /locus_tag="PKL93_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69712.1"
                     /translation="CFLYSEFSYSKELIINTRFVPYAVYCCISTEARHYDANNNPLSV
                     FASACGECRYSIDASPWSWEYVDVDCEGFGVASCNKCTIMFYFFQIEDDFEAEAVSES
                     CFCSDL"
     assembly_gap    32395..32494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            33122..33199
                     /locus_tag="PKL93_00170"
     tRNA            33122..33199
                     /locus_tag="PKL93_00170"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33157..33159,aa:Asp,seq:gtc)
     gene            33523..34029
                     /gene="ruvC"
                     /locus_tag="PKL93_00175"
     CDS             33523..34029
                     /gene="ruvC"
                     /locus_tag="PKL93_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNQ69713.1"
                     /translation="MPACVRVLGIDPGFGAVGYGVVEMRSSRIDLVSYGAIRTEPQEP
                     IPDRLLKIHDRLEELLLEFRPDESAVEELFFFRNVTTAIQVSEARGVILLAMRRHEVP
                     IFEYTPHQVKLAVTGYGRAEKGQVQRTLKTFFRMEVNPRPDDAADALAVAWCHLTSRK
                     FPGGVRKA"
     gene            34026..38264
                     /locus_tag="PKL93_00180"
     CDS             34026..38264
                     /locus_tag="PKL93_00180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HNQ69714.1"
                     /translation="MKLFMRNVGLPLSRLLSKALSIDSSFECEGIEISDISVDCKSKE
                     IELAFQCEVSNEIKEFLESVFCEKASRLLESSVTLLFKTLTSEGEIPDEDAMEVTESQ
                     FREILQLTNGASSYLRSSEMEFKDNKITISVHDQFSFQRISSKKHDLRKAIDKVCKRS
                     IPFEITLDISEDQDSPVEEVSFDREDNGETEHLEERSDSVLMGRSIRAVSVKMSEVLG
                     NETNLVVSGRIFGLEYRNGKLSILTFNVTDYTDSLTVKLIGENARSLSEKLREGDEVT
                     IRGKMETDSWMKEEILIPRDIVRTKLPKRMDNAMEKRVELHLHTKMSSLDSVVDVNDL
                     VNTLKDWGHDAAAVTDHGVVQSIPDFYFAAKKKGIKPIFGMEGYMIKDSEQISMNLEK
                     EDSPLESATFIVFDLETTGLNPKEDEIIEIGAIKMKGNEILETFSSFVKPQKELSSLI
                     TSLTGITEDMLSGAGTIKQVLPEFTKFAEGGILVAHNASFDYGFIRNSVKKYFGGDWE
                     MPCIDTLALAKSLYRAKSYSLDNVVKRLKLGHFDHHRAYEDAKVTAEVLKKFLQQAKK
                     RGVEKVNELEKLRKNQNLDSLKPVHISILSKNRKGLENLYKLVTASHTKYFYMKPRIP
                     KSLLTDFREGLLIGSACISGELSKAYIGGASVDELEEIAKFYDYIEVMPLDNIDTSEG
                     ETSMSKETLIKMYREFYKVGKKLNIPVVMTGDVHFVNPHDDIYRAAINAAQDYDNFQN
                     QPSLYLHTTEEMIGKALEIFEDEGIAKEIVVDNSRLIASQIENVVPIEGKLHPPVIEG
                     ADEEVRKMAIENAERLYGKPLPDIVEKRLNRELEAIIGHGYAVLYLIAQKMVKKSNED
                     GYVVGSRGSVGSSLVANVIGITEVNPLPPHYLCPECGKSVFPETDLESGYDLPAMKCP
                     GCGNEMLKNGQSIPFETFLGFEGDKVPDIDLNFSGEYQEKAHSFIEELFGRDHVFRAG
                     TISTVAERTAFGFVRAYSEKSGKNLRRAEQERIARGIVGVKRTTGQHPGGLMIVPKDM
                     DVHNFTPVQHPANDTKSSILTTHFAYEVIHDDLVKIDALGHDDPTFIKMLKDITGVDP
                     EKIPMDDEETLGIFSSLKPLGLRFHELGTDVGTLGIPEFGTTFVRSMLSDTRPSTFGE
                     LVRISGLSHGTDVWLNNAKDIITSGHASLSQVIACRDDIMNYLIGKGMEPKEAFKIME
                     KVRKGKGLSKEDEIEIRSCGAPEWFMNSCKKIKYLFPRAHAAAYVSMGYRVAYFKVHF
                     PLAFYSTYFSIKGGEFDIDICTSDINSIKKEIVRLRSDQDRNVKDRAKETLLEVVMEM
                     KLRGFSFLNVDLMESHPTRFLIEGNSLRIPFNRLQNMGDKAARSVEVEREKRPFSSVE
                     DLIRRTALNKTSVEMLRKYGALKNLAEKEQIRLF"
     gene            38273..39319
                     /locus_tag="PKL93_00185"
     CDS             38273..39319
                     /locus_tag="PKL93_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69715.1"
                     /translation="MDFKISASDYAKLIDLFGRVSGTLEPSYRYFQIYYKRGLKFFAS
                     DGCMKLEFSSGVATSPFNGVYTVPIDYARALKSSDPTSLIFFSAGEEDLELRTASETL
                     TLQKARSSTLPAMERKFEFFSRFPLKEFSSSLNFVSSASVEGETVEIFSKKNETVLAA
                     SANRMVLVSVLEYEPKTEFRYSLQYVTVRHLVKALELLTSEELYAGTGISELGLKIGP
                     LLFSICTDEADFSEPPSFFFGDIDDGNDVQRKAFLSALRKITRLSKHGFSVLMISDKS
                     KLKFFLKAGALRYECEVCDFVGDEFVAQVDPRKLQSVISRIKDSKIRLVTRKGNLLMV
                     GRKSKMYLMVPLIR"
     gene            39415..40761
                     /locus_tag="PKL93_00190"
     CDS             39415..40761
                     /locus_tag="PKL93_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ69716.1"
                     /translation="MGILETKGLGKNFGENNVFRNADIELESGLLTVLGGINGSGKSI
                     LVKTISGDILPDSGKVIFEGSPVDLTSIVEARRLGISAIYQEYMLVPDLTVAENILLG
                     SDKFTISRRKMNDQVSRFLEQLGLEFDPSSKVSSFSSDDQVLLEFAVAAFSRPKVLIL
                     DDLFSFVRGATRVKLLEMIDSFKKGGVAVLVTTSDPEVMVLGDRLFFMDYSGIKPLPL
                     QTKESDIWKMLGVSPEYSVAPVGGEPLIDIDNLADTGLDLRLKRGEVLCISCDNGVSV
                     REFTRALTRQDPFSENSVAMLREAKNFFERFSSRGHSSEAEKYFSSNVKNTGLVSQIM
                     LKKSRKFTSNRSLKKSFILNKESLVSVKSFLAGLGRAPGFSGLMRDDDTKLSHLRFDA
                     CDLFIFIRPLIGLDPVSTRALAEVLQEIADRSKAAVVISDEIMSRKLCTRIMNKCN"
     gene            40752..41756
                     /locus_tag="PKL93_00195"
     CDS             40752..41756
                     /locus_tag="PKL93_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ69717.1"
                     /translation="MQLNPDFSYEKKKIEFSSGYIFKGKHYRCSIIKYPSFYRNKIRG
                     TENVEIYNFSPRERAVGSIIILHGLGTVNIPFLFWMGSHLASAGLQASVMILPGNYTR
                     TATGSTSGKDFFSPDLRRLIVFWEQAVVDTMTTIDLLQQEKLWWDNNCLFGYCLGGMI
                     STIVNAIDQRINDMILMTVGGNIAKIIWQSPVLKSIRDSLRSGSGKEGYLNDEVKLNA
                     RFEEDARAVKLIKSAHELIESDLHPLFKIDPQAYARFNDRKKVTMIEALFDKTLPIET
                     RKQLWEAFGRPKRYIVPVGHVSWFPFEYALGKFILKKLGIKEARRNLRLLESTKPTME
                     "
     gene            complement(42059..44830)
                     /locus_tag="PKL93_00200"
     CDS             complement(42059..44830)
                     /locus_tag="PKL93_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNQ69718.1"
                     /translation="MRKAFSIVFFMLLSLTLFGQMKYVFSGDIDYPPFEYENERGIPV
                     GFNVDILQAISRALDFEIEIELRTWISARSALESGQIDGLLGMYYSEGRSLLVDFSNP
                     HTILHGSIFCSNRTEAFSSLNDLRESRVAVQKGDLMDELVTNEGVGSEVVRVEFPEIA
                     MRMLENGEVDAVLMSTIQGLFLIDELSLKNVMISEHPVARLEYCFAVKHGDSELLSLL
                     NEGLSIISSTGEYRQIFNKWFGNINAEDSSGDMLYWLFFAIPVASVVFLVLILMYFWN
                     RTLRRQVKEKTASLSAKLEEERSVKTQLSLSMERYRSMSETISDYYYETEFPSDGKDP
                     EIWRSESYERISGYSLDSPEPREFNWETIIHPEDLDCYKQHLEKVRSGKEERIEYRII
                     RRDGALRWVSEFSKPKYCGGGSKIKGICGAVRDITDEKLAKEELERTKDKVSKLHFVA
                     LKMEKSNEEDTIYHSIIDASVNILGMEKFSLYLLYDQELVTLESQGEVASVQGYLFRS
                     GVLRDCVETGKSAFIESSSIRKVVPNCKDSVSIVPMKSIGALVSYHDSPMKQEEIKMV
                     ETLMAHAVEAIWRIRSDREIHYVTFHDQLTALYNRSFFEEEIDRLNVKRQMPISIVMG
                     DVNGLKIVNDAFGHLEGDRLLKAVADCLKSSVRSDDIIARWGGDEFIMLLPQTDAQSA
                     ESLVNRIKKALDQVTGFSLPISVSFGIGTKSDIDQDFSETLVSAEEKMYRDKLLNNIS
                     MRNKTVEVLERSLLNKSYETEERTERIKTISRDFGKSIGLNEAELDNLLLLGALHDIG
                     KIAVPEEILTKAGSLTAEEWKKIKSHPEAGFRIALSSPELIGIADEILSHHEWWDGSG
                     YPRGLKGESIPLLARIISIIDAYDVMMSGRPYKHPVSSEEAIEELRTCAGKQFDPNLV
                     DSFVSFLGL"
     gene            44985..46187
                     /locus_tag="PKL93_00205"
     CDS             44985..46187
                     /locus_tag="PKL93_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69719.1"
                     /translation="MIALRKTLGSVDIGLIIGLVSLVLVGSLAYLFPTAKIPDPGGKY
                     EVGVEIIELDDNSRIDPFSDGEMIRRLVIDIWYPSDDTSGKRRSPWFRSHSLFVNSLA
                     DNYNLPPMLFQHLRAVKTNSFIGAAPSANANNLPVVILSPGMPAIVPLYFSFAEYLAS
                     RGFVVVGIEHPYAASVVEFSDGSQFHFNKDRVLDLPGVETFEEGARLSMELMADDLSF
                     VIDSLSKLNESGHQLAGIFDVDRVAAFGHSGGGGVVHFASNDDSRIKALLSYDPALFM
                     LNDNEIEEGIGIPSLIIETDEWKFREESGRISDLIESSVFPPLHIKISEARHPDFAML
                     DHLSPLAHFLDFTGSFMKSGGEYYLYDIIQSFLEYTFGNLSESELLSRLLQRDDVRVF
                     KGIHEIPR"
     gene            complement(46735..47571)
                     /locus_tag="PKL93_00210"
     CDS             complement(46735..47571)
                     /locus_tag="PKL93_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ69720.1"
                     /translation="MRVLFLGTAAYEGHPNVFCDCDNCRKVREAGPDNFRLTSAVLVN
                     DDLLIDFGPNIMAGAQKTGSTLFNVRTLLITHSHSDHLYLPNFGYRMDRYNASYERLS
                     PMSVLANSTVLSLISDSVYFDPDKTILLMAEAYKETVLNDYSVIPIPAVHKVKEGEQA
                     LLFLIKKKGKSFFYATDTGPLDEFSLKLIKSFLDSPLDLVAIDSTLGFMKEVTFPYHQ
                     TAEQVVSTVETMRKNGIMDDTTKIYAHHFSHYPNPTQGELERFYVRFGIAVAYDGLSL
                     EI"
     gene            complement(48198..49763)
                     /locus_tag="PKL93_00215"
     CDS             complement(48198..49763)
                     /locus_tag="PKL93_00215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ69721.1"
                     /translation="MDHIKQEKSFADLYEEYLSKDESEFDSRISFLKRIQRSLVDSGE
                     TQIAFEIVGRELEKIGNQCSSELGFVYNSMIRISCQSGEFSKALEYAASAISVFDELG
                     DDSRLPMIYNNIAGVYLKMGEFETSLEYIDKAIDLSQRNNDNHSLAKYLNNKGIAVEN
                     IKGDGSGAVFIEKAIELKEENSLKSDLPNSYMNLADIYTYTGKEDEAHSLLKRAREVA
                     NEIEDEFSLAEVCRYEANYYRAVGELGSALESLNTSLEYHKSKGNKSEILQRLTVISE
                     IQEIVGDVKGALETHREISKLNAEIFKEEEARMVAKLQSSFSTMQKLKEIESMARRNR
                     QLDEANRLIVQKNEELLEMQSQLSSANQLLKERAETDSLTGLMNQKKMFEIVEYEINR
                     AKRYGNRLSIIMLDIDDFKVINDTFGHQKGDGILESLSSLIISSIRKIDYAFRYGGEE
                     FVILLPSTGVEGAFSTAQRISESLKRTNEIPVTISIGVAEWSDESVNDLLLEVDTLMY
                     EAKNTGKNRIVSR"
     gene            complement(49778..51358)
                     /locus_tag="PKL93_00220"
     CDS             complement(49778..51358)
                     /locus_tag="PKL93_00220"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ69722.1"
                     /translation="MEDNTEIKDRVVELLTEYMSVPESDLSLKITYLNKAISSLNHQG
                     NTLVAHGIVEREIRRFDNCDSLEMAVLLNSMIKLSALVGDFAGVVEYSRRAQTICREK
                     EEEGLLTRVLINTSGIYFKMKEYDKAFSNANKALQMARKNGDETNTACCLNNIAVILE
                     NSETDKSGIPYLEEAILIKEKNNMKDELPNSYLNLAELYYDSGKKKEASELLQRATTM
                     ARENGDERALTETMKYRARFLKAEGELFSALELLTGVSEYHQKNGNNTELLDVLNEIS
                     SIHELMGNANEALQTFKLITELNRTIFKEEQARSLAQLESSFEARENLREMHSLLEQN
                     SELRRVNEEISEKNNELHDMKRRLEKTNKLLERQAETDSLTGLLNHRKMRTIVENEIA
                     RAERYGTPLTMIMIDLDNFKFVNDSYGHLKGDEVLSEIGRIIKSSIRKNDFAFRYGGE
                     EFLVLLPLADLDKATEVYSRLKESFREELEITVSFSAGAREWNGESADKYIAVVDKLL
                     YEAKTKGKDRLETSQATS"
     gene            complement(51459..51794)
                     /locus_tag="PKL93_00225"
     CDS             complement(51459..51794)
                     /locus_tag="PKL93_00225"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNQ69723.1"
                     /translation="MFFTLAVIIAVVVVAFLIKGLSEKDEVAKREEEREISFSQHAVQ
                     RMDERGIETDRVIRVLEEGIRVQIANYNRLKVSDDEMTVILEKRLDNFEVITVYWNDD
                     DHKDADLIP"
     gene            complement(51833..54343)
                     /locus_tag="PKL93_00230"
     CDS             complement(51833..54343)
                     /locus_tag="PKL93_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ69724.1"
                     /translation="MKKVFIALLLMLFVTPICLSSAVWEDQVVYFLMIDRFSNGDPSN
                     DDMGLGEAGSDNSRYNGGDLKGIIDKLDYIKGLGATAIWITPPVANQWWNPWVNYGGY
                     HGYWARDFKRIDEHFGDIALYKELVEKAHEKGLLVIQDIVPNHVGDYFRFVDGEFEMN
                     SGSVPSDSPEQYPFSLNNFVEHRDENIYHWTPDISNFNDQDQKFNYQMSGLDDLNTEN
                     PSVISALKDSYTFWIDEADIDGFRIDTVIYVPHEFWREFLNGESGVYEIASKNGKLNF
                     LTFGEAWVRSDPFEDTGERVIGEFFESGMNAMLDFPLNIELRSVLKEGKPTANLGYRL
                     ETRQSYFDHTRLLTFIDNHDMERFLKGAGLASLKQALAFIFTVPGIPVVYYGTEQGFV
                     ETRAAMFEDGFQSGGLNHFETQSELYKYTSDLAKLRLEHPALRYGRVEVLKSDPNGPG
                     IFAYSLEHDNEKIYVIMNTAGERRILANMESGLAEGQVIKPIYTFNSLSKSYPVDENG
                     KLLMTMNPRSVYVGVASEEYQSVESPEIQFIANLEYAQKIASTYTITGTASGASSARI
                     IFDARIEEAEDIEIEDGQWSYEWDISKFDPGIHSILFKVYGRTKRESIYSEDYTVVLD
                     IPELLLSSLSDPEGDDKGPEGRYEYPTDISFKNQMDLLGVELRQIGASLSLNMRIKDL
                     TDTWGPQNGFDHVTFQIFIDDPHKTGSTVLPFLNATMPAGLDWDYFIFANGWSIVAYS
                     AEGSGPKSFGTAISPTPLVQTNKMESEVVLRIPGETIGRPESLNGFKVYITTWDFDGI
                     EAVYRDIYPEPKSYHFGGGSKEDPYIMDDILLKIGESD"
     gene            complement(54363..54932)
                     /locus_tag="PKL93_00235"
     CDS             complement(54363..54932)
                     /locus_tag="PKL93_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69725.1"
                     /translation="MKIMIDIDDILTNFNLAFLRIAHKMYDEVPIEVEVKIWDFWKSV
                     PNLTLEMEERVWERIRNTPNFYESLPSYANDEDLRRLGELIAEGQHEVYFITSRFPTK
                     GRNVQIQSQRWIQKEIGQPVSVIVSSRKGELCEVLGIEFAVDDAPHHIENLLDHGINT
                     YIMDWPYNRHIDHRLRVKSLGEFLDSVLI"
     gene            55127..55795
                     /locus_tag="PKL93_00240"
     CDS             55127..55795
                     /locus_tag="PKL93_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ69726.1"
                     /translation="MFELKEVALIYETKKQALAALKSTNLIIEQGEEVGVFGPSGSGK
                     SSLLFIMSTLKEPSRGEVKYEGKEITALTPSAKAELRRKEFGFVFQEHFLINHLTVRE
                     NILVPIRDKQGIKRRVEEITTALGLEDLLKRFPYELSVGQSQRVAVARALIGNPKVVF
                     ADEPTASLDDENGRKVINLLRSYCSKSGATLILVSHSAEIIADFKRRIKVENGQVYED
                     TVNA"
     gene            55788..57962
                     /locus_tag="PKL93_00245"
     CDS             55788..57962
                     /locus_tag="PKL93_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ69727.1"
                     /translation="MLKYIWLYFRRRPGRILVFIIVVALGVAVSIVLSSIFLSFGETR
                     SRISKINENWITVQYLSDTGRKEEIDDNLKEIFADVSGISDVIQVDIAYLGYNLFGSA
                     RANFPVYGIKQTDIARILQVSQSYIVDGRIFSPKTNEIMVSDSFLKASGVGIGLVYEE
                     IFETASRGLYNVVAALKGPSVFGIGPSGISGGKGAYGFIVLSERGFSIAVESELRSRI
                     AGEELKVGVQGPISSRENIERSYSGYYLGVLLCNLCVALVFIIGLTMLNSVSIRERRK
                     EYGILAAIGHSPASLGARLFFESVYQCAAGWIIGLFAGRILVSYLETRFFEPNGLFVG
                     DIFVSTIWTLVIPAVTVVFTQVLIGSHLSRDLVGLLKSSEKKKGILKNRFKNISLPWL
                     RFPLRSDGYRSLFVNIIVFALLVTLFGSLLSSLTDTIRDSGRFFDSCSYVQTMESGDI
                     ELPEEIVSRSRAVLPASLLNLDVKLIFGTTEIFIPVVSSKNSELEQTLVEDTSENAFY
                     VSKGLFDLINSGEVNGYGNSSPVVLPSLYGLSGIVVEEEVAAEGLLIFHAGDDLVEEI
                     RLFASEAGVAEVVDRRSFEAKILKESQFMNLITSIIIYLQFFVVVIIAVVTVTRIVLG
                     RRRELAIRNIIGQGRGEIALLLFGELILVLFSGATIGFILGTMSWSIFKAFFLSGVYI
                     SSFVAAKVFLKIFIMILIVLFSGLAMERYLISKQDPLSIIER"
     gene            57971..58966
                     /locus_tag="PKL93_00250"
     CDS             57971..58966
                     /locus_tag="PKL93_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNQ69728.1"
                     /translation="MRFRELVSPPDAYETGPLNKITDLEGIEVGHFTLIEDAPRCLRT
                     GISVVRISGVYGSPIPAACSVFNGYGKSMGLVQIEELGTVESDIFLTNTLSIGAVQQG
                     AAKLALEDNPEMTSLNVVVMECNDGYLSDIRSLSIREEMVSKAVMDARKDFALGSCGA
                     GTGMICFGYKGGIGSSSRIIEFDGRKYTVGAIVLSNFGRSSDLRIPSLELSNDSGSSA
                     QGAGSLIMILGTDLPLMPHQLKRVSRHMSLAIGLLGAPGHHGSGDISLAFSTAREYSL
                     RDQRLMKNENTISEIFRASAWACVEAIVDSMLSSNSMTGFKGSVKSLKSAVIRKP"
     gene            59208..60476
                     /locus_tag="PKL93_00255"
     CDS             59208..60476
                     /locus_tag="PKL93_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNQ69729.1"
                     /translation="MIDANPAALETEGLTREAAIGMDLEHVFPPAREFSVMDGVRRVN
                     ETGIPLHIGSKHNPETRSEVWRDVSISRLSSNVIAIVYNDFDESSEARKDLKKSKLEL
                     DRLFSNLPGIAFRCKNDKDWTMEVLSGGLVDLAGYRPEEIIGNKKLSYADLIFSEDRE
                     KVWKSVQESFTRDDHYEIEYRIVTKSGIVKHVLERGKVIKNPGEDQSFVEGFISDITD
                     LKAAKIEIEESKERLENTFKSTVNALSKITEIRDPYTSDHQRRVGLLAEAVSRKMGLE
                     RKLCENIRISGLLHDIGKLWIPSEILSKPGRLNKIEMEMIKEHPRLGCEVLGEIEFGF
                     PIAEYVIQHHERMDGSGYPNRLKGEEISLPARIIAVSDVVEAISSHRPYRPALGLNVA
                     IEEITSGAGTLYDPSVTRTCVDLLKNGFSF"
     gene            complement(60481..60837)
                     /locus_tag="PKL93_00260"
     CDS             complement(60481..60837)
                     /locus_tag="PKL93_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69730.1"
                     /translation="MKGRLKDISGEIFVVLGTILVALAFFMVFTWGDKAVVMTEITVI
                     KKIDNSSAGNSDYRIISESGEEFTVRNAESQGFYAASKVFRMLEVGRTYQVLVTGRRI
                     PLFSIKRNIIEAIPSQ"
     gene            complement(60864..61901)
                     /locus_tag="PKL93_00265"
     CDS             complement(60864..61901)
                     /locus_tag="PKL93_00265"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNQ69731.1"
                     /translation="MNTVYLRVNSEEPDEFRLKKAARFIREGELVVFPTETVYGLGAN
                     ALDESAISKIYHAKGRPQDNPLIVHVSSREMVADLVLQNLSEYSALMDTVWPGPVTLI
                     FRKRKRIPDSVTAGLDTVAIRFPSHPVASKLIELSCVPVAAPSANLSGKPSPTREEHV
                     VEDMDGRVSCIILSGSTVFGLESTIIDLSRERPVLLRPGPIDPDQLKCVLPDLQIPGF
                     ASGREQYLGDPLSPGLKYRHYSPDIPLILVEGELGLVVSKVGIIAGRKSSLVLCSEEL
                     RDYYPVQVKKVVLGSRSNLLEVASNLFKELRNKENMSFSQIIAEPFPETGVGLAIMNR
                     LRKAAWKIERV"
     gene            complement(61916..62155)
                     /locus_tag="PKL93_00270"
     CDS             complement(61916..62155)
                     /locus_tag="PKL93_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNQ69732.1"
                     /translation="MISPEAFEKLLAKVGLLCLAIALLLFIFRKAGLSLGRLPGDIHV
                     SRDGFEFWFPITSGFIVSVCITGMLWLWKFISKFF"
     gene            complement(62440..62898)
                     /locus_tag="PKL93_00275"
     CDS             complement(62440..62898)
                     /locus_tag="PKL93_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ69733.1"
                     /translation="MIIREIRETDAPGLHEVLVDIGWFKMKNVEEEIYLKSVKEMIKW
                     SIERDDHLILISEEGGELTEYTSVHFLPYLFMGKPEGYVGELFVKTVWRGKGFGSELL
                     RRAINEARERGCCCIRLINIRDRESYRRGFYAKQGWQERTDAADFMMKLD"
     gene            63327..63416
                     /locus_tag="PKL93_00280"
     tRNA            63327..63416
                     /locus_tag="PKL93_00280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63361..63363,aa:Ser,seq:gct)
     gene            63432..63508
                     /locus_tag="PKL93_00285"
     tRNA            63432..63508
                     /locus_tag="PKL93_00285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63466..63468,aa:Arg,seq:gcg)
     gene            complement(63519..64241)
                     /locus_tag="PKL93_00290"
     CDS             complement(63519..64241)
                     /locus_tag="PKL93_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNQ69734.1"
                     /translation="MDLKPTRRSFMIRYFIYPYYFVLGIVFYLNFDLSGIDGNNWLFL
                     IVSWIALTVIPGILLAFSRKKFGWFFWPAILNASGWLVRYLFPEETYNDLKPLFDNGP
                     VLLFIVAGLLGIVFVEIYRTTFKYNLSDAGVEVTYGMFRANSHMIVARHITNVMLKRN
                     FFELILGVGHVIPVTSSGMGSGDRGVLGGFSADTGTQNVRGGAFIGSVSTEKEFVANP
                     KNCIYGISKPKEIMERLKEMVL"
     gene            complement(64231..67140)
                     /locus_tag="PKL93_00295"
     CDS             complement(64231..67140)
                     /locus_tag="PKL93_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ69735.1"
                     /translation="MNKDIFVTASAGTGKTFSLVKEYVGVFDRSFRIGERLDVHNVVA
                     ITFTNKAAREMKDRVITEIDHRIASGHPGSWKPLRNKMSYAWISTIHSFCERILRESA
                     LFAGIDPGFQILSGMRRATLEEKVVRTYFEDNLESLEPLIQLTGLDKAFRIVKEAISN
                     KRHSLALNPSPVGSLLGDSIISDDEIIEGSKSFAAAYSEILSDYRDRTVRSGFLDFDQ
                     LLTETRDLLLKMPEVQEKYRDRFKFIFVDEFQDTDELQSEIIRLLREETKNRVFFVGD
                     SKQSIYRFRGADVSVFNKTMKSFQNNGGELRNLRINRRSHPDLVTFQNKLFGKIMKRD
                     FENEFFRSIYDEDIESLPYQRDIESSRVRIIRTESSDDSREVARSVRALIEEELVFKT
                     KTGEYEKRRIVPGDIAILLRTFSKISSYENALEKLNIPFYTVGSRNFYERPEVSGLLS
                     WLDLLVDPLDNNNLVSFLLSPAFGASLDEVLSLRSKRDLRRLPLYFALMESKEDRFAH
                     LIDLLKKFGKLKYILSPSEILEKFVQETDYLPKLATLKSGERMIANVKKLLELAKDLD
                     RMGSSLRELSSNIKAFIDSSDESEAALETEESDSVKILTVHKSKGLEFPIVIVGDTFW
                     KKKNSDRRLFFHDGGFLISKSKPDREEGLIGSLASLEEEKEFEEEKRTLYVALSRARE
                     MIIISLNGRSDSSRPWSKMICSSLLQPESDKVEASFEGIVKHFYPIDLPSIQETVYIE
                     EPLLPEVTSVLPLTDSSYIKYLSPTSITADDEFELESESYAGSPGLVKNAADIGLLAH
                     YLLEPVGVISKRGSVTTLESILNGGRPVSVDRINFNEYDLSEVKKVLSTLVAHPLIKE
                     IEGAERVFSEFHFQHGFGKYVLMGIVDKLYLKDGKWRIVDFKFASLNKRFINKYRFQM
                     NLYLYILKDLLSPIEATLLFLRDGKTEKIMLEDSDSFEKELLQMISKAGGGKNGS"
     gene            complement(67133..70210)
                     /locus_tag="PKL93_00300"
     CDS             complement(67133..70210)
                     /locus_tag="PKL93_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ69736.1"
                     /translation="MKITLFIGPLASAKTERLLRELETVHWRDPFSYFFVGPSGDHVR
                     YFRENFISRVGTIPASRFLAMDQFAVEVFRRLNPNSIHVGNHLMRLEIGDILDQMGKG
                     ELADSPVFVEYLLEMIHDVKENRGFDRIFSTEDEAVSVLESIYNELDSRFSRRNIFDT
                     FDAYTRIEEHKDELINEEFGDTLFLDGFHDFSPALKIFFKTIIPTYREVFITITQEPD
                     KESLFSNIDSILETIDEIAENSTDLEIKRIFFEKSSSSSGLNGFKEALFSPGKVEQNC
                     DSVSVVVYPDIFAEVEGVSRFVKESIISACEPGDISIVSSDFNLYSKLLSDKLNEYGV
                     PHRIEGDEELHSSLSIRRLILPLETAVLGFPPEKIIAMADSGYGGEVDSSFIESAAAM
                     SRIIYERPNMWLSLEKRRSSWDERLSRLVDLMKERRNAIISLADDELEMSAAEELDET
                     IERIDSEVVPAVQRIFRLLDSFRSLRKRDVGNYRKMFITWEKELSLIQSLTGYESEVK
                     DREMDAIVRFFEHTLPYLERLLYFMGKSSVSPREYYRYLMLLLKHDKFPLSRNKANRV
                     EIQSLINSRFSKKRMKIFVGFVDGAYPHVEMNPLYSFTQFGDSRPRDLLLDEETHERL
                     NLYLSISTAEESVRFSLPESTIDGEPILPSPYLKNVAESSGCETTREGRKVGRRSGYI
                     PELRASMSQSELKISAVKFFLNEKWDLLKSFPPLSNLDTVLSNFRRDFSWSVQNRRRL
                     EEVVGNVFSFSRLKSYHDCPFSFFLSYVVGLDVSPEYVFELTPLDEGNIFHSVLKDFF
                     SGKNRDWQDSLAENIKRHLMHDSNIVFRFEFERLSEVLHEYITVREGKRPNFMGDNFI
                     PYAFEKAFGIGETEPVKLLENLYLRGKIDRVDIDRSSSSVYLIDYKRGDSGDERQLLL
                     YSLVAEKLLEDESLDVAGGVFKTLTGKTVNKAAFRTISSEGGKVWEFAGKKKDNRIVK
                     ESDLLKWLKEVHEGIYSGEFTPSFTVSASKCYSCRFSEIKRSITWREGRKEYE"
     gene            complement(70964..72223)
                     /locus_tag="PKL93_00305"
     CDS             complement(70964..72223)
                     /locus_tag="PKL93_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ69737.1"
                     /translation="MEIKRDGQFYRFAAYGFLKNLRFFDPFLILFFREMGLSFLQIGT
                     LVSVREVATNFLELPTGIFADLFGRRLSMVFSMISYLSSFLVFYFFPNFYIYMVAMIA
                     FAFGEAFRTGTHKAMILEYLRINDMTDIKVHYYGATRAASQLGSAINALIAAFLVFYT
                     GSYKIVFLASVLPYAVNLVNLMMYPKELDGELKSGEKRETLKAFLGIFRSANALKGVL
                     NSSLYDAFFKVIKEYLQPILKALALGLPIMLGFSPEQRTSVIVGVVYFVIFIATSYAS
                     KNAGRLSKKLGNEARSLNYSYIFGAILVILSGLFQIVSLQVIAVIVFFLLYILENFRR
                     PINISYISDNIDHKTMASGLSVESQLKTLLVAIIAPIVGFLADKLGVGMALTVAGMTM
                     LVLFFIVKLNPPENTKNIEEKSSRTAD"
     gene            complement(72432..73796)
                     /locus_tag="PKL93_00310"
     CDS             complement(72432..73796)
                     /locus_tag="PKL93_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNQ69738.1"
                     /translation="MTAIYTPEVWYFLVMLATFVGLAIIKIPISVSLMFSALAGSIAF
                     GEFFPLRHLVEGGFGYIDTILVIGSAMIFMESIKVSGLLDTIAGNMTIAFHKKPTFLL
                     VLMTFFIMIAGMITGSSTATVLTTGAIAFPVLKTLGLSKRRAGSIIAMSGIYGMIAPP
                     INLPAMIIGQGVDMPYIGFTNPLLLMTFPLAIVTSLIIGVRQVKPVDLQEFVRTRERE
                     PIKGFAVYLPLIIMVILMVLENVSWGFSLGLPLIFMISAIVASLTGARGNLFKISLSA
                     VQNSLGILSILIGVGMFIQIMTLNGVRGLIVGFLNGLPQGALLVGIALGIPAFGAVSS
                     YGSASVLGVPFLLAFLGNNDIVVCSALSLLAGLGDMVPPTALAGIFAAHVVGEKNYFK
                     IWLSSMPAFFITMIFATLTIIFSNEIGRFANSWLFYPLFIAGLVVVSVALLLVDKRKE
                     MSVT"
     gene            complement(73807..74874)
                     /locus_tag="PKL93_00315"
     CDS             complement(73807..74874)
                     /locus_tag="PKL93_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNQ69739.1"
                     /translation="MKIKAIFFIVLLSTSAVFGAFLRPDIRPGYGVTDIGWLSDYFSP
                     IRNTNMDTPVYFMDSGVEGPTFVLMGGTHAREISGTTAAIVFIENVIVTKGKVIVIPF
                     SNLSAASIKDETGHAPHFIAIEGKSGPRFLVYGDRRTDPLDQGIPDPEIFKHAQSDFT
                     LEDGAEARNLNRQYPGVADGNPTQQLAYAIIQLIDTENVDFNLDMHESGTPDSYIDSE
                     GNERSGSSLAYTLVCHPDALEIGAVAIMEIEAYYGVSMKLEESNRAFRGLSHLEIRDA
                     TGSLSFLSETPNPGQDNWRDKYDVIDDPDYPLTHRAGLQLQIIRSLFSAYEMMIGETV
                     EVENLPTYEEVMQNGIFSFLN"
     gene            complement(74931..75497)
                     /locus_tag="PKL93_00320"
     CDS             complement(74931..75497)
                     /locus_tag="PKL93_00320"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNQ69740.1"
                     /translation="MKRFLVSLLLIACVAFVGFAADGTEEGLILGEPVAVTSAGQSPG
                     ALQFTVVAKMIKLEYTFEKLLSVDSIDISQFKTLVLVVGASGKGLGAANIDIEAEILR
                     VKSLAEAAKENGLKVVICNLEGESRRGPSSDRIVTELAPFADAYFVKSDADQDGFFTA
                     LSEEAGVPMATFEKTVDLRDVLTKYFGK"
     gene            complement(75521..77260)
                     /gene="ggt"
                     /locus_tag="PKL93_00325"
     CDS             complement(75521..77260)
                     /gene="ggt"
                     /locus_tag="PKL93_00325"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNQ69741.1"
                     /translation="MKKLVLLVTLITFVFTATFATVTAPTVASSNGMVAAVDKYAAEV
                     GARILEMGGNAVDAAIAVSFALGVVEPYASGLGGEGYAVITMADGSKYAIDFRSTAPM
                     AASYEALSELGLTLSKANYTPKGACVPGVLAGIKEAWLLGATLPLADLIQPAIDLARN
                     GFIVDETFAQTTSDKYELVLENGFDFLNEGFVWEVGSTFTNPQLAETLERIKEEGIDT
                     FYTGSLADEIEEFMIENGGFMRKSDLEMYRAIVREPLHGTYRGYDFYVPHPPVSGPQM
                     IAVLNTLENYNLQAMTWDDPLAVHIIQQALVLGDVDRRAYISDPEFYDLPYEAFMSKE
                     YAKTRFMAIDLSQAFDPASYYDYQGDAYPFADGSTYEEVLLGALESVAANESGVEESP
                     STTHFSIVDKDGNAVAWTQTISSFFGTSVYFKGFFFNNEMYNFASSYREGDVINLTGG
                     MRPRTTICPTVIQKDGKVRWVIGTPGGGRIISTMVQLAVDLIDFNMPVDQAVRTPKFV
                     GYTTYKDLRIEEGYPQTTLDFLEKVLGHKLNVYSYPDLFFGGPNLISVEENGLMIGAG
                     SIRRGGSASAPEF"
     gene            complement(77293..78423)
                     /locus_tag="PKL93_00330"
     CDS             complement(77293..78423)
                     /locus_tag="PKL93_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNQ69742.1"
                     /translation="MMRLKRHFKFLAFTASVAIVVILSAFSFTAMWEDPPIIPGSGVT
                     EIKMLSEWLPSIEGTMLDTEVYIIRGAEEGGRFLLLGGTHPNEPGGTLAAVVFVENVS
                     ALSGTVYVIPRSNHSAFTHNDAMEGAPQFFSIELPDGSERVFRFGSRRTNPIAQWPDP
                     TIYLHPTGATLGGGEVSNLNRTFPGRENGYSTEKVAAAIMNLVLEEDIDLVCDLHESS
                     PEYPVNNAIVFHQKAAELAIMTQMMLEIEGIDIRLEESPVNLRGLTHREIGDNSDAYV
                     VLLEVSNPSQGRLRGKTTDELVTEGIDKYYLQASKDGKLFAPYDENGYPLDLRVARHV
                     ATIRVLLDSWNMMFPERTIFLSDVPPYEGLVDGLGTYLNPVS"
     gene            complement(79332..80603)
                     /locus_tag="PKL93_00335"
     CDS             complement(79332..80603)
                     /locus_tag="PKL93_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNQ69743.1"
                     /translation="MMRNLIFVILILSSVISLGQEDILKTINETEELSIVASYIQLSG
                     LDQTLSGKGPFTFFAPTDSAFMKLPELTRADLIKDVVRLQRVLKYHIIQGKFSSSIIV
                     QRPNFVTLLGQPLSVDTKGGIKVDGSSILVRDIEATNGVIHIIDTVIFPHEEPDIVQV
                     MRNTGILDTAVFSLEETGVIDILKGEGLYTVFMPSDVAFARLPPETMENLFRDPARLS
                     EILLFHIVKGSFSVEELFERGSIRSLLGETIVLGTTPPRIGPQKTVVILSDIEASNGM
                     INIVDHVMFHEDSRTKLSTIEETLKANEIDIMAELLKKTGICSELSENKRLTVFAPTN
                     EAFLELKESDKAFLYSANELRKVLEYHILPNEYLLSELKISNMLLSLIGKPLYFEMKE
                     DLLVNSIPIVESDILCSNGVIHIIESLLVTK"
     gene            81132..82898
                     /locus_tag="PKL93_00340"
     CDS             81132..82898
                     /locus_tag="PKL93_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNQ69744.1"
                     /translation="MRSILINGEVAYNAPSQIFNSVTFKEILAELINDSEEKKNSLLS
                     LFEPFLRNTDTMETSRKYDIDGIVELLLALTIDPIEKIDGSSYYSFPKMSNKRELLVR
                     FVESLFSLWRNKHRFIVKRDEYTPNRFDRIFKQMVLVRTNTDLKSLVLSLYRQILINV
                     SDIRLKILRQEPGGAQVGFIVDNPEVDKRARVPGADWLYGMACVWSIVFEPPVIFYTR
                     SNKRHGLFEVRDKPILSKLNIPSSNDWFLFPIHVGSKLIDVVVNKEYLSHAAGLGNLF
                     ELASFDIVKNKKPDGIYILGIDNSFFEKKEDFNGVIYREDDGTYIGIVGDDPSIDYFG
                     YMKKMILTIHNLLVIDEGRLPIHGALAHIKLRNGKKANVMLIGDSGAGKSETLDALNR
                     LHAEVSEVNILIDDMGSLDITSSGSVIAYGTETGAFVRLDDLQPGYAYSTMDRSIFMN
                     PNEINARVIVPYSNYTDIIKPTKVDYFLYANNYNEDKDSDLRIFKDAEEAAAVFSNGA
                     RMAKGTTTEKGLTYSYFANPFGAIQRRERHEVIADRYLKAMIDSGVRVGEVSTRLGIP
                     GYEMEGPLMAAKALLKAIDEQS"
     gene            82956..83882
                     /locus_tag="PKL93_00345"
     CDS             82956..83882
                     /locus_tag="PKL93_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ69745.1"
                     /translation="MVELPRNVVEVKDLKKYYGETKAVDGIEFDLREGEVLAILGPNG
                     AGKTTTVEMLEGLRKPDHGTIEYFEERLPPSSDRVKEEIGVQLQSSSFFEYLSVKETL
                     DLFRGLYRKSIPSKDLIREVSLQDKEKTYTKNLSGGQLQRLAVAVALVNDPRVVFLDE
                     PTTGLDPQARRMLWETISALKNGGKTIILTTHYMEEAERLADRIMIMDQGKIIARGTL
                     LELIDSIDVENIVTFSVQGDTPIPEGFLEVEGLQHTENREYSVTTRDVERTLGRLFEI
                     SKEFDMLLDDVTIRKPNLEDVFITLTGKKLRD"
     gene            83892..85055
                     /locus_tag="PKL93_00350"
     CDS             83892..85055
                     /locus_tag="PKL93_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ69746.1"
                     /translation="MSRMWNFTSGLFKSEIREFQVMFWSFIFPLILYFILTSVFGSLY
                     GDDTQGISFRVGIVREESLSGLGMILDKVIEDISSENGPFVKKEYGSVDEALIDLKEG
                     KQDIVLVIPEGTSAKIASAAVLQTGEVALRVHYISGKDSSSIASNVLSEIIDEVNLEI
                     RRQSGGEYVDVVTEAKTVSAREEKPFDYREYIFPGVALMMILSVALFNSPIGLIQYRV
                     SGVNKKLYTTPLKPLEYFAGHFAKLIFTMLISLTLLYLTAIFVYNVRGPILDIRFILT
                     LLFSMVVTISFGLMIASFSKKLSTVTVLGQTLYQLMMFLGGLYFPVFHLPWGIRWLVY
                     ALPTTYLVELSRRVMGYQFAPVSLFWLILVPIIWTAFSTVIFVINFKKVMGYE"
     gene            85048..86112
                     /locus_tag="PKL93_00355"
     CDS             85048..86112
                     /locus_tag="PKL93_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ69747.1"
                     /translation="MSRNSLKPVFIAFAKEAFRNRLEIFFTLFYPMIFLLLFGFFFGG
                     SESYQKVSVGLYQTGPYDISAVIEESGAWNLRLYDGETDIGEAVKNGDILLGMIFNGE
                     KITYLHQEGNLEQQSTIEMARASISAAVEKEINNARSFIVVENIPETAGRIHATDADY
                     TMSGVIAISLLSAGMFSVISVFGRYRKRGVLDRFKITPLRPFTFVLGSTLTRFLVSLV
                     SIVLILVVSRLLFRTTFQIDWLPFLVSILSSTLGMMALGLFLTLLFRNPETADAAASI
                     LMVIVVFFAGIYFPLSFLPDYLRVISIFLPVKYVAELIRHSLGIEFVSTSYFVITNLV
                     LFLSGVVLLYFTGKRYLSAS"
     gene            86199..87392
                     /locus_tag="PKL93_00360"
     CDS             86199..87392
                     /locus_tag="PKL93_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNQ69748.1"
                     /translation="MSDTVRIAEGIYWIGVNDFETNLFEALWPLPRGVSYNSYILIDD
                     KVVLIDTVKGPFFSDFLDKIRSLLPEGRKVDYLVVNHMEPDHSGSIKVLREAFPEIKI
                     IGNEKTKGMLDAFYGVTDNVVTVKDGDELEIGSKTLQFHMTPMVHWPETMMTFEKTTG
                     LLFSGDAFGGFGALNGGIFDDEVDIDYFEDEVLRYFSNIVGRYSSMVQKAFEKLKNVD
                     IKVVCATHGPVWRKNPSRIIELYDRWSKEETEEGVVLVYGSMYGNTQKMMEAVASGLV
                     KGGVDKIRVHNISTTHISFIIRDIWRFRGLVLGSCTYNMELFPPMKQLVSAIENRMMK
                     NRKIGVFGSYTWSGGALKELQEFASKSKWDQVGPVIEVKSCPTQEDLENCVELGLNMA
                     SAIKK"
     gene            88001..88864
                     /locus_tag="PKL93_00365"
     CDS             88001..88864
                     /locus_tag="PKL93_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HNQ69749.1"
                     /translation="MKKLSLSIILLITLFFAGCMEFPSQEPDRGTVRVFLTDAVIPVE
                     DIDSLMVRIDSISLYGDEHQPVVISDTPFEVDIVTLVGATYELPNTISVSGIYNQLRM
                     EVGAATLTANGEIFPVTVNSGSLKINDLYLTIDENATTTIVLDFDLSKSLIINGRWED
                     LKIEEDNGGFNSRKDKVHMTPVLHVRHGSLFDVTGVASSSIYPLLVALFPESGGEALT
                     TFTHEDNPIWDQGEFRFCKVVSGDYRIEFFDNYTDEGFSIDESESRYQPLFVTVVDRD
                     VDLGTIVLVEK"
     gene            88973..89629
                     /locus_tag="PKL93_00370"
     CDS             88973..89629
                     /locus_tag="PKL93_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69750.1"
                     /translation="MKKIVWILIIMSLFFSSKILSNVLIEGERSLLDYLVEGKALNMA
                     VDISLIQSVQEKVPGEIQAVFLLPEGSVRVITDSHFVTLSETGEIYEIYELTGSGTTL
                     KVGIIDALSLVSVQTRENVFGIFLVQEENSYFWHVVTGTRTFRLASSFASTLSIKPNE
                     EALEEINKGLQKSEEVRENQHPSEKQGKPEDPGKPEDPGKPDDPGPPNTPPGKENGKK
                     "
     gene            89636..90322
                     /locus_tag="PKL93_00375"
     CDS             89636..90322
                     /locus_tag="PKL93_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ69751.1"
                     /translation="MKLLVVEDEKKLLENVAAFLERKGFLVDTAQDAEDGLDLALERN
                     YDCIILDVILPGMGGYEFCRFLREEIGSDVPIIIITALGEIDSRIEGLKIGADDYLPK
                     PFDLRELEARVRALIRRNQLRSEPVIRYGPLTYDSRSGVFSCSKKELSLTSRESSILE
                     LFLRNPGTVFSREEITDKIWETGFESRSNIVDVYIRYIRRKLEAAGILDIIQTVPGKG
                     YGLRRMRDDG"
     gene            90312..91586
                     /locus_tag="PKL93_00380"
     CDS             90312..91586
                     /locus_tag="PKL93_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNQ69752.1"
                     /translation="MTVKSKITLLYLFTYGSIVVSLGLTVFFVLKNLEMRRIDNLLIS
                     FHNDIVNTYKYAGEENRLLTLAGDEDLGFAIYTDEVPIASFRAEPEFFKAVTGSGTKG
                     DYRFRSSFEIIEGKEEKFVTFYDLDESQKYLRFVFVTIVIFSLLTLLIVSVVGMVFTR
                     KLINPFEEAGNQLERISRNGLKNSRVELRKTGEEVSKLEREINKSLIRIEQLIEEAKQ
                     MSSKIAHELRTPLAVMKTNLQLSLTKDFSEDTMREALKSTLGEVDKLIRLSEDYLLLS
                     RTESSLPIEKNLINFSQLLLQTTEKILMIHSDKDLEIDIAPDIVIRGSGYMLEHVIIN
                     LLDNACRYTTDGSVEVKLISDEHGSFLSISNMGKAIDFVQNEELEEAKQIKGYGLGLR
                     VVRSILRAHGLKLDYTHLDGKNIFVINFPFEE"
     gene            91780..92811
                     /locus_tag="PKL93_00385"
     CDS             91780..92811
                     /locus_tag="PKL93_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ69753.1"
                     /translation="MTIKELLQMAGESSSEFLDTWLGYTEVSGHPILRKEISRLYSTV
                     GEEDVLVHSGAEEAIFNFMNVFLQKGDHVICQFPVYQSLYEVANAIGCEVTRWELRRI
                     GEEWSFDVDELKDLVRPETKLVVINNPNNPTGYLVPNRDLMKIASFTEERGIFVFSDE
                     VYRGLELDQSVEKQKSFADISDNSLALGVMSKSYGLAGLRIGWVVSHNKSVLESMTRF
                     KHYTTICNSAPSEYLAWKALCVGDKILKRNRAIVVQNWDRAAPFFEKHSSVFRVSRPK
                     AGPIAFVQFVGGDVEDFCSVVLESAGILLLPGSMYDFEGGFFRMGFGRKSFAEGLERL
                     DEFLGLFGK"
     gene            94288..94422
                     /locus_tag="PKL93_00390"
     CDS             94288..94422
                     /locus_tag="PKL93_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69754.1"
                     /translation="MSSEEGSLFHTRGIDLIETCKGGKEGDILEWSVRQQPGGQRDGE
                     "
     gene            94412..96136
                     /locus_tag="PKL93_00395"
     CDS             94412..96136
                     /locus_tag="PKL93_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ69755.1"
                     /translation="MENEKKTVRRFVFKLFKVYWKRELLAAVLVGVMIALAVVFPLLI
                     RLLIDVVIPSGDMDLLVKYVLLVVGLWFGSLIFTYFGEVVFETTALMAKSDMRKQLLE
                     KMFVLPFDFFHKNKTGELVSRLISDLELVGTVIAQVFPILLLGVVQITAILAIMFAFN
                     WKLTLLPLGFIVFSFLVIMFFNIMVEKRSKVEREKFGELAGVTSNIIDNMKLIRIAMP
                     FSWVTNFFDRFQGDHVYAGKRFIKAIKIAGALKTSINGLISFSLLAYGGYLVMMGEIT
                     VGILMTFWAYVQSLFNPIQLLMQVNILLRQSWGGLLRTIEVLEAPEEERSDSSPKLES
                     VRSISLENIAFGYGKKQILKGLSLELRKGEITTLVGESGAGKTTTLNILMGLQKPQSG
                     TIYINGDPVSNSALLRPYIGFVEQTPTLFEKCTLLENITLGRSISKASLENIFERTNL
                     DVLLRRFENGLDTLVADIQLSGGERQLVALARALVSNPRLLILDEITANIDSKTEEII
                     QNTLLGLRSEGIIVLMVAHRLSTVLLSDRVALISNGVIVANGTHNELLKTSSEYKKLY
                     SLQLIKDE"
     gene            complement(96559..97104)
                     /locus_tag="PKL93_00400"
     CDS             complement(96559..97104)
                     /locus_tag="PKL93_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69756.1"
                     /translation="MKRIAVFVLILGMASALVATHFSVGMGFVLNQNRQTKEFDLQFF
                     GRTQVGWMLFSKPVLGIIADVPVFGFGFEEMEFYSLDPTQSLIGLYAELGLEDLASSL
                     PPVFFKVTAMTELSSILAARDIKYLPLVSHIGAGFDMSDFSLELATSIINVGFNLGDQ
                     LARHSTFFPHFHLAGRYNFDF"
     gene            complement(97129..98322)
                     /locus_tag="PKL93_00405"
     CDS             complement(97129..98322)
                     /locus_tag="PKL93_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69757.1"
                     /translation="MDNFKPRVLRRAEMEKELEKLLNVEAEIRSDSYLLTFYFPMENM
                     TRADAERHVKSFILEHLRKDEKLSGMGKINQRIVEAVLTGLGNTKELRRGLAIFVEFR
                     MGEDVKADEVIEGLSIIPLSQVPEKEIYIGKTFDLDQLIWLSSSAIDALIVSLEREKS
                     SFYAMDGNKLSLIKSIENEFIRKKEQEYIEEYSPSPSSGAMYHGNASEKVGRAKEEEN
                     RRFLQKIASLIKNDEDLPKDVNYLVIYYSSSFNETMEKFKNEARQLLPYAHPVFVQKT
                     LNQERQLQEDASKLIESVSRKIRREFLHNAKENFEKYVEGWTEVSKAANDRRIDTLFI
                     NPTERKRGYVLGRELVYTHAVKDSREVRNVGPWIVRSVVNNDGKVVVVDKELLDCEIA
                     ARLRY"
     gene            98486..99766
                     /locus_tag="PKL93_00410"
     CDS             98486..99766
                     /locus_tag="PKL93_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ69758.1"
                     /translation="MIRLANSLLCLLHSRMGRAFPALKVKNFRYFWIGQLISVMGTWM
                     QTAAQSWLVLTITDSPFLLGLLGVAQFTPVLLFSLPAGVFVDRFPKRSIVLLTQTISM
                     ILALILAILVFTDSVQYWHIFLLALGLGMVKTLDIPARQSFMIELVGRDVVLNAVALN
                     STVMNLGRVVGPAVAGVVMALVGAGWCFLVNGVTFLAVIFGLLKIDVEPVIKKRPKGT
                     VIPDIKEGLVYLFSKPILTTTTLILAIVSTFGMNYSVLIPVFARNQLGLDAQGYGLLM
                     SALGVGSMLGALIVASSSSRGPNRVRLFVVPFITSSLFMIMAFNRSYAFSALVIGLMG
                     FANIFFTTTANSLMQINSDDEFRGRVMSVYSLVFAGSTPIGNMFVGSIADKAGGGSAF
                     FWAGVITFFLVAATIIAHKIRKRSLRVKPEEHPD"
     gene            99820..100776
                     /locus_tag="PKL93_00415"
     CDS             99820..100776
                     /locus_tag="PKL93_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNQ69759.1"
                     /translation="MSSIPFSRISRFWGLKRKIVLEKKTNARAFIVRSGSVFYVLKRR
                     IASLPSRSCQTELLKYLVQSGLPVSAPIPSGSGEFEVKIGKYVYSLFPYIEGEKADSN
                     ISNATPEMVEELGIFTGRLHKVLKEAEGSFFERTNLLKSLTSAIRYFNSTGSFFSRSD
                     MEMIEEFFTDFYKLYYKLPVQIIHGDYHPGNIIVHEGKVSGIIDFDCATREVKALDLA
                     YLIHSVFAEFSRMGSPSLFLYLLPHLLEGYCLENTLSFDERESLGYLLAAISIIQIHY
                     FSANGLSDEANFASTVFRWIYLNNHRIKEKSGLVLTRSEKTV"
     gene            101133..102632
                     /locus_tag="PKL93_00420"
     CDS             101133..102632
                     /locus_tag="PKL93_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNQ69760.1"
                     /translation="MIGLKIVIDRNSEVPFFKQLVDQLRTAVIRREIPEGTRLPSERE
                     LSKLLRLNRSVVVKAYNELKLDGLLVSKTGSGTYVISAHSQKKEFDMISWGEQLNSWA
                     TLPAGPARWVNLLKKLSDSCIRLDTGVPHVGANQVDLMKEILGNLTDEELITTLNYPS
                     LRGHPELIKYLCLRMNSSGLHINSRNMLITVGTEEAIYLLFSALARPGETIVIENPTY
                     VNVINISRMFQHRVIPVERNSEGLNLSTLENVLNRSRVKFIYTMSCSHNPTGANMPER
                     KKEALVKLSEQYSVPIVDDTVFSEIQYDLPAPKSLKYYDTHNCVIEIGGISKAFAPGL
                     RIGWIIADEALIERLIEPVRMISLGVPNFSQLVAAKMLSTGEYDAFLKRYLQLCLKKR
                     ETTKRACYRHLPGYIRVNEAQGGNFFWLELPGFMDGWKLAEEGIEQGVAVAPGSLFTF
                     DSSGRSFVRLNPLGIPHDQIEEGISRLSKAIESISKRDNREEGTVSVVV"
     gene            complement(102643..103737)
                     /locus_tag="PKL93_00425"
     CDS             complement(102643..103737)
                     /locus_tag="PKL93_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ69761.1"
                     /translation="MKRRLPGDKLIWRALRPILRVPFIKRYNLYGIGMEKLPRPPFIL
                     IGNHAYFIDAVLIEALTSFPIVWAVAAGNFDNPFSGPILRAAGAIQKRKGVPDIPAMR
                     KMIEVVSNGGVLGLMPEGSVTWDGEFGEVPPGTDRFLEKLNVPVVAARMSGAYLTKPR
                     WADHHRWGRIEVEFKAFNGKEALDFLSEASDWRWQEEKKVMFKGRNKAEGIEKIIWFC
                     EKCGGFQTIKASGDSAICDKCGTSLTVDDYGLVSGRTVPEIIGIQREILAEHIALNGG
                     IEVGEGTVTEMEIGSGAKTSYAGKVSMNSRELRIGERSYLLPKIRGLSNFLKRITEFN
                     YEHSIVRLMTGSSSLLLFWAHRYFSHPHGS"
     gene            complement(104264..104629)
                     /locus_tag="PKL93_00430"
     CDS             complement(104264..104629)
                     /locus_tag="PKL93_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNQ69762.1"
                     /translation="MKLSSEGVLHGSFGGTEEAGATTVRGSPSFDAFGNVFFGTNGGV
                     LYCISSGGKELWNFETGREIWSKSVVGIDGTVFSCIMTTGGSDSNYIFALKGGEFLWR
                     YETQTIYSGFFSSPAIGED"
     gene            complement(104629..104784)
                     /locus_tag="PKL93_00435"
     CDS             complement(104629..104784)
                     /locus_tag="PKL93_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69763.1"
                     /translation="MNTTDTIFSFTSIHDSYRKVFSLESSSGEWFSSDASINSEGDLH
                     IGTTSGN"
     gene            105224..106513
                     /locus_tag="PKL93_00440"
     CDS             105224..106513
                     /locus_tag="PKL93_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNQ69764.1"
                     /translation="MGLMDRIFIVVYFILTFILIVLSKRRRVLIAFTAGMMLVALKVS
                     ESMRIETISQFVDFNAIFLLIGMMVIVAVIKSTGFFQYVAVRTLKATKGNILLLSVLF
                     SALIAAFSMVLDNVTTMIMFMPIIFFVADTAGFNPFGFTAVMILASNIGGCMTLVGDP
                     PNIIIGNASKIPFLTFTSLVFIPLVLTYIVLMLISRFKILRGLSSISDKKDEIQGLKL
                     DGVITNRKLMYVSLGTLIVVVIGFAVHSIVDIEMSLFAVLGAAFLLLYTGKDFESVAN
                     EVDWNAILFFIGLFSLAYSLESTGITSELSDLALGLSSNPAVLSMLILWVSGMIAMVT
                     GAIPVVTIFIPIVAELSVHYPLQYDLWIALALGANLGGNGTVTGHLANVMCFEMVNKE
                     YGNTHSFLDFMKIGFPSSVISLAISSLFLIIRLVIFG"
     gene            complement(106523..106966)
                     /locus_tag="PKL93_00445"
     CDS             complement(106523..106966)
                     /locus_tag="PKL93_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HNQ69765.1"
                     /translation="MSPALFSIGIAISTAFWWVTGLSAGGLVTPVYLFIFMEQPLRLL
                     YTWAVGLVTFLILNLLQRYLILYGRKRMALGIVLGVFVKLSLDTFLMPNLPVELFSTV
                     IGTIVPGLIANDFYRQGIIKTSLSLAFVTLLLWLIDTLIRTVMVI"
     gene            complement(106966..108069)
                     /gene="pgsB"
                     /locus_tag="PKL93_00450"
     CDS             complement(106966..108069)
                     /gene="pgsB"
                     /locus_tag="PKL93_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HNQ69766.1"
                     /translation="MINIILAGTALLILLSALLAENVISRKRRSRLKVVVQVNGTRGK
                     SETVRLIHGALKANGFRVLGKTTGTVPLWITPEGKHVEIIRHGPANIQEQFLALRKSR
                     RDKCDALVVECMAIKPEMQLASRRILDADITVITNTFPDHIEEIGSTEEETASVLSLS
                     IAPGKACIVGSVSPEAYEKVRKTCEKIETKLIVGATSTEYLSGFKFKPHDENLSITIK
                     AAELLGLDRSLAIEGMKEVLPDVGTFRYLKLGGKMGNAVLANAFAANDLHSTSLLLEK
                     VMRDFPEKEIIGFFNSRDDRPDRAAIFETMIRSFDRTIVKGPLPRRILNSKNVEKLDD
                     PSQLDSLLDGSELVFGFGNIRGLVNWLNGLEEI"
     gene            complement(108066..109169)
                     /gene="pgsW"
                     /locus_tag="PKL93_00455"
     CDS             complement(108066..109169)
                     /gene="pgsW"
                     /locus_tag="PKL93_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HNQ69767.1"
                     /translation="MKSSHVLLTVPYSRANISLKWLLLGAMIGVFGFFLVGQSRQRIE
                     SPYYADQVAAARLMASSIEELSRFRESLGIEIDPSVDPNLTGLIGPEFTEITTTLGNL
                     QAKRTSTNPDFAALMVKFFKQLNLEKGDPVAIGASGSFPSLLLATLCACETLELQPFV
                     IYSIGASEHGATHPEFTFVHMLNRLVEVGLLRDSLIAVSLGGNYDTASGMFFPGAREL
                     MTNIALSSGKTFIYEESLQESIDKRLEIYIQEADGLPSVFVNVGGAAPNFGNSLIATS
                     LDNGLLTAFSSIPDGDERGLIFEFAELGVPVIHLLNIRDLALDNGIPIDPVPFPEPGE
                     SGVFFVDSFSLPLIIVFLCLMVLCIVVGRVIHK"
     gene            complement(109200..110822)
                     /locus_tag="PKL93_00460"
     CDS             complement(109200..110822)
                     /locus_tag="PKL93_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNQ69768.1"
                     /translation="MKNGGMSLEKGSSKQLTIRSLVIGGFGSVVITTSSMYVALRMGA
                     LPWPTIFVAVMSLAILKALGRTNLNEINVTHTAMSSGAMVAGGLAFTIPGIWILEETS
                     TVGFLSLLIVTLSGTVLGVVFTSLIRQHFVEKEKLPFPMGVASYETVVAGDEGGRKAR
                     YLFSTMGITAVFVAIRDGLGWIPAAWSSAWLYSKNIFFGTWISPMAVGIGYIIGPLFT
                     GVWFLGAVLSYFFVIPVGVALGWFEDVASAAAFKDSLGIGLMVGTGLGILIKGILPRA
                     KEIYGRSQKREKGRLGRKLILAPIFFAVVAVFLTTLTEMTLIPSLLTIVGVWLTTAMA
                     ASITGQSGINPMEVFGIIILLGVKVVSDIGTIEAFLVAGVVAVACGLAGDVLNDFKSG
                     YLLKTDPKAQIIAETVGGIIGAVVSVIVLFIMFRAYGTMGPGTELPAPQAYAVSTMVG
                     GLPNTPAFFFGLMIGILIYLIGLPGMTLGIGMYLPMEISSAAFLGGMISLISVKLNPK
                     SKENGLVISSGLLGGEGITGVVLAIIRVLTGS"
     gene            complement(111218..111382)
                     /locus_tag="PKL93_00465"
     CDS             complement(111218..111382)
                     /locus_tag="PKL93_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69769.1"
                     /translation="MSTMLAYHDTGTGKAVFYEGDFFPDKLANCEAEREEIIEDLLKY
                     AWMRIEDLYV"
     gene            complement(111573..112205)
                     /locus_tag="PKL93_00470"
     CDS             complement(111573..112205)
                     /locus_tag="PKL93_00470"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HNQ69770.1"
                     /translation="MLSQIFDATLETLYMLLLSGFLATFLGIPLGVGLYLCSRSRGLK
                     GLYALLDLLVNIFRSIPFIILVLLLIPVTKRVMGTIIGPNAAIFNLTIAAIPFMARLS
                     ENSFNGVPSGIIDSSNSMGLNRWQMVTRVLIPETLPDQIGNVTVLLINLVTYTAIAGA
                     VGAGGLGQLAINYGYYRFQWDVVLYAVIVLVAISQLLQFTGGKLAKRLRK"
     gene            complement(112205..113194)
                     /locus_tag="PKL93_00475"
     CDS             complement(112205..113194)
                     /locus_tag="PKL93_00475"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HNQ69771.1"
                     /translation="MILRVEKLNLSFEDKSGKRKILDDVSLSVEEGEIVGIVGLSGAG
                     KTSLLRTLNLLQPVDSGSIFFDGLDIVGLKESEVRNVRKKIGVVFQSFNLYRYRTLYQ
                     NIAFPLKIQGVSKKEVEERVMNIATELGLEHRLRAYPSQLSGGEQQRAAIARALVMDP
                     KMILLDEPTSALDPKTTCRLLDLVVSLNEKHGITFVVVTHDMDVIKRTCDRVAYLQNG
                     KLDFFGPTHEFFVKIENNEVSDFGKPLELEKGLLSANKKILRVLFWGTRTHEPVLWKI
                     AKENDVMINILYGKIEEFKNGPFGTLIIGVEGEKQDLFVEKLRESVFFLEEVC"
     gene            complement(113206..113985)
                     /locus_tag="PKL93_00480"
     CDS             complement(113206..113985)
                     /locus_tag="PKL93_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ69772.1"
                     /translation="MKRLFVLLVLLGVVFAFGTTKIRIGASPVPHAEILEAIKADLAE
                     KGFDLEIVVFNDYVLPNIALSTGELDANYFQHLPYLVSFTSQRGIKGLVSAKAIHVEP
                     MGFFLKKPLSELAPGDKIALPNDPTNEGRALILLHNNGLIQLKDPTKLESTVLDIKEN
                     PKQLTFVEIEAGFIPRVYTDDKTVVGAVINTNYALTINLNAITDAEFIEGAESPYANI
                     IAIREVDKDEAWLKALIDALTTEKVRVFINEKYDGAVVPVF"
     gene            complement(114234..114608)
                     /locus_tag="PKL93_00485"
     CDS             complement(114234..114608)
                     /locus_tag="PKL93_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69773.1"
                     /translation="MSIGEASLDSRSFKFSNEGSVDVTSFAKQVGIKLPVVISKNVWN
                     EMVKTDENARMYGQNEEKRLESLLSTLRMELLKGKAKDLSFTFFICKDPKTTSCRLLK
                     ACYKDKDNGERYLKICTYDEAS"
     gene            complement(114754..115170)
                     /locus_tag="PKL93_00490"
     CDS             complement(114754..115170)
                     /locus_tag="PKL93_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNQ69774.1"
                     /translation="MKSYRKELFFNTKMRREIINITDDVEGALLESGIREGLVLVNAM
                     HITASVFINDNESGLHQDFERWLERLAPEKPYSQYEHNGFEDNADAHLKRTVMGREVV
                     IAVTEGKLDFGPWEQVFYYELDGKRRKRVLIKIIGE"
     gene            complement(115192..115758)
                     /locus_tag="PKL93_00495"
     CDS             complement(115192..115758)
                     /locus_tag="PKL93_00495"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNQ69775.1"
                     /translation="MSGQLLFFIVLFFWVGFDIYLFFLHNRKISERLQEKRSKYIMLA
                     FVLSGMFGAIAVDGRSKEVFLMTFLPHRWIGVGMMIFAVVIRFFVVRQLGPAFAIDVG
                     ALPDQKLRTDRFYKVIRHPAYAAEIVGFLGLSLVFNYPLSSILAFVLPTTGILYRISV
                     EERNLKVIFGEEYREYCKKTWRLIPYLF"
     gene            115922..116347
                     /locus_tag="PKL93_00500"
     CDS             115922..116347
                     /locus_tag="PKL93_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HNQ69776.1"
                     /translation="MIFGRDESVLLFRFEDGEDFIDTFVRTLSDNGLHSLIVVSGIGM
                     LRDFTIGWFNSVSKQYEKELVTVPHELLHISGNVSLKGGKPFPHLHVTLSGPSRSTVG
                     GHLFGGTVCNTTELFAITTGTLKLFRDAGSETSKLEFRK"
     gene            116307..117524
                     /locus_tag="PKL93_00505"
     CDS             116307..117524
                     /locus_tag="PKL93_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNQ69777.1"
                     /translation="MPAARHQSWNSESELADLRFLHCSDIHLGRRPIGSPNSPYSYTR
                     YEDYFDAFEFVVDWAVAHNIDAFFITGDLFDKREINPDTLARTQNILSRLKESEIRVL
                     AIEGNHDKSFNVNESWLRFLHETAYLELLSPVQGDNDILFPYVEISGFKVYGLGYPGF
                     LAEEMISKVASILSGRNNIVLVHTAPGNDSFIPGLVTPEALNILRSKVIYAGGGHLHS
                     QLAFPADDPFFFVPGSLEYWDVWENDRKGFYVFDTEKESADFHDSHRRERQEFSVQVD
                     ENERLSDFIENHEIHQGAIVLVTVSGRGDFNVDQGSLEREIESCGALKAFVKVRRTDR
                     EITLDDYRSMSARDIEREVIMSSKEWKEIGAKGEELVAAIDKMKHFQEEGRYDLFKDS
                     VDSFLERIIGDVP"
     gene            117521..120343
                     /locus_tag="PKL93_00510"
     CDS             117521..120343
                     /locus_tag="PKL93_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNQ69778.1"
                     /translation="MRITSIDLENFRSHSRYSGTFEKGINLILGRNGSGKSSIIEAIG
                     LALFGGGLRDRQEDAVRWNERRARIKVAFLADDGLEYVVEKVFGNASNHELYQGELLV
                     ARGRENVIEKIRTICGLQGDISRTFENVIVAFQNKIADQFLGSPAVRRDYFNRVFQVD
                     LYRKISTDFMRSYVSDLRNEDEFLRREIAILDSQLERKEEVKERMAQLLKDLSLTKKE
                     LDTLRDTLTKIRSEKTGLEEVSRELASRKSSLERELVSLKDASGNLKNNLKQRERAKR
                     AKEIVDESREAHDLYEKLLKEENILNTEKRRLEGLAEESSRIGRKISEFEIEITRLTE
                     SLSNKNNRLREINERFEAQKMERSGLIGEKEAAELESGKKRMAYELSVSKKKAFKVIY
                     EQYRENERRINALEDLWASVQNLESVEELNLTLQKIDEEIEKTNQEVRILEKLNEAKT
                     TKSAQKKALKEYEGSLAKGICPLLNERCRNIENREDYGGYIAELIKGLEEGERELLKE
                     IKKVEDTPSRLNELLGEKTLTLKAIEERAKKESEKSRIEEEKSKLMKTHKKLILELSS
                     ISGVPAEVEQMSEEIEREIREASTELELQNKQVSSLADQIKRIDSQIEELIIGRNILD
                     EEKKETEKAIASLRTETANLRSSLEEYAKELDKLPEFNTRIEETRRTMALNKAGYENY
                     NRFATLAGYLEEYASLCQSSLEKCRNTSLSISKIRKEYSSLQRAFDSERFEQLRIEEK
                     AVEEKYNLKRDETVSIGRDLLSAKTDLKNLADIEKQKKTRQKTIEKIGKKVKLAEKIR
                     ENLNLTGERITAGFRDAIERKATLDYMRISGMEETIRWNEDYEVSVKAPEYEGGSIRS
                     FSNLSGGEQVLVSLCLRAAMTGILSGARFAVFDEPTSNLDKERKELLAHSLRELFGEL
                     DQSIIVTHDDVFSEMAQKVIRLEPAE"
     gene            120367..121734
                     /locus_tag="PKL93_00515"
     CDS             120367..121734
                     /locus_tag="PKL93_00515"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNQ69779.1"
                     /translation="MRSLKTILKNIDYLVTMNEKRDELEDAFVVVQDGVITDVGTGTP
                     SPGDIEIDLSGRIILPGFVNTHHHFYQSLFRSVKEVASAKLFDWLVFLYERWKNLDNE
                     AIYVSSKVAIYELMMSGVTTTTDMLYLHPKGRSEFIDSEIEAARSTGVRFHPTRGSMS
                     LSKKDGGLPPDSVVQSEREIIEDSVRLIEKYHDRGKNAMLRIALAPCSPFSVTEESMI
                     RTAELAEKFDVLLHTHLAETRDEDDFCIQKKGLRPVEFMDKVGWLNRRSWFAHLVWLS
                     DEDIRKLSENDCGVAHCPSSNMRLGSGIARVKEMKEAGMRISLAVDGSSSNDTGNMLL
                     EIRNALMLQRVLKGADALTPRDVLEFATLGGARVLRMDDFIGSVEVGKSADLAAFRLD
                     TLSMAGGLSDPVASLVLCDPHKSDMVMVNGKIRVQNGRVIDSELPGLIKRHNEISSKL
                     IGGEY"
     gene            121788..122045
                     /locus_tag="PKL93_00520"
     CDS             121788..122045
                     /locus_tag="PKL93_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNQ69780.1"
                     /translation="MEATRENHKKSVSEPGNLRFDFLQDKADPSHFFLYEAYESEEAA
                     ANHKRTDHYKQWKERVEEYMERPRRGTQTRVIEPERLRDWK"
     gene            122082..123194
                     /locus_tag="PKL93_00525"
     CDS             122082..123194
                     /locus_tag="PKL93_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M24"
                     /protein_id="HNQ69781.1"
                     /translation="MLIEEFRRRIDGLREEISSRGAGAFLISKCCNFSWVTFGARSHI
                     TLNSTEGEASVLVTLDRVYIITNNIEKQRIQEVEFHEELLGEFGFLEYNWFETYGEKR
                     LIDNLLKGKLLSDTGRYDSEYVDLTEMRTVLSVYEIETYRALGRDCDDIFSSIIPGLK
                     RHMTELEVQGLFYEAMSKRDIEPILTLVFSEDSSLSYRHNLSRNVKLDRRGFVSICAR
                     RRGLIVSSSRSFMFEPVEEISLQHERNCFVDAVAIDSSKPGERLSNVFEKLVEAYEHV
                     GRGGEWKLHHQGGVAGYLPREVHGNLKSDFILKRGNAVAWNPTIRGTKSEDTVLIGDF
                     GDSNISFPQTSSWPELKYEFDGSTVRRPAILLVDKV"
     gene            123397..123840
                     /gene="smpB"
                     /locus_tag="PKL93_00530"
     CDS             123397..123840
                     /gene="smpB"
                     /locus_tag="PKL93_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNQ69782.1"
                     /translation="MKTVVTNKKARFQYHLMDSYEAGIELVGTEVKSLRLGGASLIDA
                     YCKIESGEIFLVDCNISQYTHGNVWNHEPRRKRRLLMHRKEILRLDQKTKEKGLTIIP
                     LKIYFNDKGKAKVEICLAKGKRLFDKREDIAKRDVERRMRQQADL"
     gene            123986..124549
                     /locus_tag="PKL93_00535"
     CDS             123986..124549
                     /locus_tag="PKL93_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69783.1"
                     /translation="MKKVALVALLLFAVSLGFSTPKALIGGAFGINATNPVYQPERWG
                     AGAFDLSLQLPFTDKIGAMLSAQAAIRYPSNKIGLFNADIYFQIFETLNLKARLLVSV
                     GSVQDSNFGEQSWEIGILDLGAPHLAIGGGFQVMTPISDKVILNGFARGYRVKDYQTR
                     QEGDFPGGDYWPLPEFFSVGLGVLYEF"
     gene            124586..124888
                     /locus_tag="PKL93_00540"
     CDS             124586..124888
                     /locus_tag="PKL93_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69784.1"
                     /translation="MVVFVLLFSIVSLAVTGYDKFIHYSVSYSAYGLSSYFLGDIGGF
                     VFSASLGVGKEIWDWFSGKGTAEYGDLIADFAGIISAYSLTKRLPFRPLLVFVLVF"
     gene            complement(124915..125997)
                     /locus_tag="PKL93_00545"
     CDS             complement(124915..125997)
                     /locus_tag="PKL93_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNQ69785.1"
                     /translation="MGKPVAYENLRKLCEEILLSENFGIEAAHEIVDVLLEADLRGIP
                     SHGVARLGRYIKERDSGNILLDSEPSIVHDTPVSATVDGNGGPGQCVSNYAMDLAIRK
                     AKESMIGLVSVRNSNHYGISAYYSEKALSEKMIGIAMTNSYPLVVPTFGKEAVLGTNP
                     FSVAIPGLKKNLILDMATSVVTRGKLEVYDRVERDIPNGWAVDETGRNTTSPRRVLNN
                     FNSRNPGGILPLGGSEEDFGGHKGYGMALLVDLLSAGLSLGRWSAETYSGSQAGVSHF
                     FGCFSLELFGDRSSLIEHLEHIVDGVVSSKKADGHNKIYYHGEKEAMARERAVTSGID
                     LDQKTKDSLVSLAKKHSLSIPVELLD"
     gene            complement(126000..127103)
                     /locus_tag="PKL93_00550"
     CDS             complement(126000..127103)
                     /locus_tag="PKL93_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNQ69786.1"
                     /translation="MKLGDKELEKLLLELCSANTTNPPGNEDRAVEILQRVLVDRNVH
                     IEIQKVAENRSNLVARLQRDQALPYLLLSGHMDVVPAGSNWDSDPFKPELVDGKLFAR
                     GSADMKGGLSALTAALIDLSEDSDFSGNVALVATCDEEVGCSGIRHFLEHQTFDVSGV
                     IIGEPTSLRLATGEKGAIWLKLKFRGKSAHGSQPQNGINAVTRLFSAYTELSQVLGKI
                     EGLTESLNIIRGGSKENTVPDEAECVIDIRFAENTDSAEIIALVDSVLCKYNQSEKII
                     LLSRESFSSSGNLTESVKEVLKEKAMNAEELTMTYFTDGAFTASQGIETVVLGPGSPS
                     MAHRSNEYVELEEVHVARRLYSKIARNYFERTR"
     gene            complement(127100..128275)
                     /locus_tag="PKL93_00555"
     CDS             complement(127100..128275)
                     /locus_tag="PKL93_00555"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HNQ69787.1"
                     /translation="METFKGYLRSDGSAGVRNHVLVLPSVLCSANVARRISSAVPGSV
                     VASHNQGCAQLGDDFKQTRRTLINTALNPNIAAVLVVGLGCERVSPHELRDIIQDHGK
                     PAEVIMIQDYGTVEAIERGIAIVKDMVKEVSKIERKEVSLSSLVLGMECGGSDFSSGL
                     SANPVVGQVADFLAKKKGRVILSETTELVGAEHLLFERMSDESMKQRFTKMLQRMIDE
                     SLKNSRDVVDKENVPNNISPGNVRGGLTTLEEKSLGAMIKGGKVPIVGVKEYGEVVDA
                     EPGLYLMDTPGYDVESVTGMVAGGATVVLFTTGQGTPTGHAIAPVIKITGNRQTAKKM
                     SDNIDFDCSTIIYGEETLEESGRKLFDLVLRIANGELTKAEVLGQDDYSIWNVGIKL"
     gene            complement(128285..128584)
                     /locus_tag="PKL93_00560"
     CDS             complement(128285..128584)
                     /locus_tag="PKL93_00560"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HNQ69788.1"
                     /translation="MKQAIMLSVNDNVATVLEKVAKGEEIEMKGTSQVGTIVAKEEIP
                     FGHKIAVRDIRKKERIIKYDNKIGVATADIATGQHVHIQNVASERTEIKEGGERA"
     gene            complement(128587..129312)
                     /locus_tag="PKL93_00565"
     CDS             complement(128587..129312)
                     /locus_tag="PKL93_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ69789.1"
                     /translation="MNRSADTNHFHGIQYQLYRSIRNSIVELEIPPGSRLTIDRLKRI
                     YGVSSTPVRGALHKLKEDGLVAPGIAKSFYVKEILEEDVRKLFPIRLVLEKLALSESI
                     FRIDRETISNLIVRMRKHLAEPEETKNNVPYDIDYRLHREILSNCNNEYLIEMMSKIS
                     ILITRMRNVIKYYLLEQQKDWIICEMEEHFKIAQGILEGDLEKASNALETHLIHSMGM
                     ICSILGSNNVEYTARGSFVELED"
     gene            complement(129309..130139)
                     /locus_tag="PKL93_00570"
     CDS             complement(129309..130139)
                     /locus_tag="PKL93_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ69790.1"
                     /translation="MAVSFMVANDDIPAGAKVTGLTGRLEETIPALVEAGFRFFELMI
                     ADPGKVEIEKVKRLEREHSAKCIFLCTGEMAGTAGLYLNHIDISERRRAVNSFMSAAR
                     KAYELGVDLNIGRLRGVIWSDGLQKSLDRLAESLTEIDTFVKNELEGLSVLIEPLRKV
                     VCPILNNCQEAADFFETRGLSSYGILVDSDHFNEEVDSSFVSSNIGLVKHVHLADSNH
                     KPLGQGTIDFHKFLNLLTQSGYRRNFSVEVFCDEDQIETLNDTRIFLRELSLLKEGSF
                     "
     gene            complement(130150..131052)
                     /locus_tag="PKL93_00575"
     CDS             complement(130150..131052)
                     /locus_tag="PKL93_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNQ69791.1"
                     /translation="MISSLSVFTHKVKDLDKKIRILCVQPHDKSTVEAIRSIAESNSK
                     IEVTMYGSFEKISEITPAEFLKEVQIVEGETEEENASRAAGSVNGETPAVLMKGNINT
                     AVFLRTLFRSENVKTAGRLISHISCMEIPEHPKLLFITDGTVNVAPDLREKAEILRNA
                     AGFARSIGLKCPKIAVIGINERISVTNRDLIEAAVLSKMAERGEFGECLVDGPMPLDT
                     ALKKEAALNKGIQSEVGGDADILVCSNLESAANLIKGLVHLGKSRTAGLLIGTGFPVV
                     LTSRSDDEFAKMASLSIAIVSAAG"
     gene            complement(131063..132103)
                     /gene="buk"
                     /locus_tag="PKL93_00580"
     CDS             complement(131063..132103)
                     /gene="buk"
                     /locus_tag="PKL93_00580"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNQ69792.1"
                     /translation="MSLILVINPGSTSTKVAVFRDTEEVFKKTIPLDDDMLDLPLFPD
                     QYTIRKRQIVKELDLNGVRPSDLAAVAARGGRLKPLESGVYRVNKEMVDDARRGLQGM
                     HPANTAVVIASEMAEEFGIQAFTVDPISVDELAEWARYTGINEIRRNSLSHALNMKAV
                     ARKAATKLGKNYEDAKLIVAHLGGGSSVSAHSNGRMVDLYNSDKEGPFSVERAGALPS
                     HELAEFARERDDYLDYLTHRGGLFSHYGMRDVESIARRVMKEEIDSAPLEAYIYNIAK
                     YSYSLLAVFDGFPDALALTGGVVKCDFVRERLLSKLKGFRVLLFPGEYEMESLAEGAH
                     RVISGSEEALDY"
     gene            complement(132100..133353)
                     /locus_tag="PKL93_00585"
     CDS             complement(132100..133353)
                     /locus_tag="PKL93_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HNQ69793.1"
                     /translation="MKPKDLEYMKLNGTPYFDDSRWVCPLNYENKIRGKDAKDSIYIH
                     DVTLRDGEQTCGLTWNEDQRVRIGVALNELGVKSIEVGMPIVSDENKRAIRRLVDMKL
                     DSEIVPFARSLKKDIDESLDCGATRVVIEHAVNPYDNELVYGVSPEKLVDRVVSAILY
                     AKEQGMKPTFMGWDATRSSFDYVLKIFTEVAKQAKPESMVFVDSFGVASPFAIEFIFS
                     ELRKAIPDIPFEFHVHNEFGLAMGSVFAAVRAGVSGIHSSINGLGERTGNVATEEVAA
                     GLKLLMGIDTGIDLSKIGYTSRLVEDISKIQVANNKPVVGKRLFWVESGIVVDAIDKL
                     NKAGINAAMTPYLPSLVGMGEIQVKLGAFSGQASIKYWLKRLGIEANEDQIEELTEIV
                     RKEGRIRNTVLEIDEFKRIVGDFMK"
     gene            complement(133355..134599)
                     /locus_tag="PKL93_00590"
     CDS             complement(133355..134599)
                     /locus_tag="PKL93_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNQ69794.1"
                     /translation="MEFSLDYREGRRVKLEVPAGQVILPAISAEPAEAGSSLTESISV
                     PIGTAPLTSLAESAKSIVFIVEDHTRHSPVLETLDLLRKLFESRGISWDKVSLLIATG
                     THRQMKPEELQEKFGVFSSDTRIVQHKAEETAKMKDFGEFLEVPIQVNEAIEADLTVG
                     IGSIVPHRFSGWSGGAKIVLPGVASYESVFKSHRMAILKSKADVGVFENEFRELIDET
                     GSRVGLDFIINFYYDINGSTAGTVAGNHVLALRKGVELARKQLLRQYRERTDVTVISS
                     FPSVTDFWQCGKALYTSDLITKDGGDIVMVSSLDEGFGDHPLFASLLKLEANEILEKL
                     DMITTEDPLAYVAAYAVRKVLQKKKVHIVSDTKFAEKFDELGLRVNPDIQSVVDRLIC
                     PGKSVSVLQNSLVLPEITTEEV"
     gene            complement(134601..135317)
                     /locus_tag="PKL93_00595"
     CDS             complement(134601..135317)
                     /locus_tag="PKL93_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ69795.1"
                     /translation="MTLLETRDLSVSYNEVPALRRVSIKVMKGEVVSVLGPNGAGKTT
                     LLKSISGLVGSEPGSKILLNGEEIQHLAPHKITRLGVIHVPEGRQVFAELSVQENLEV
                     AATRVGLSKARPNIERVYELFSELKSRKNQMAGTLSGGEQQMLAIGRAIVSDPVIMMI
                     DEPSMGLAPVIVKRIFETLKKFVKDTGLTMLIVEQNAKLSLPMSDRVYIVSQGQIKLE
                     GSVDEVKSDERIREMYFGGK"
     gene            complement(135320..136078)
                     /locus_tag="PKL93_00600"
     CDS             complement(135320..136078)
                     /locus_tag="PKL93_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ69796.1"
                     /translation="MDILRTENMTKRFGGVVANNDLSIGIPEGEITALIGPNGSGKTT
                     FINVVTGVHPITSGKVYFKDQDITGMEINRISRLGIARTFQKIRLFENLSVIENVLVA
                     RKSFYKSGPLSVVLGTPSFRKEEKENREKAMELLELVGLEDNAYDSPKGMAYGLRRCL
                     EIARALALEPQLIFLDEPAAGMTRDEFKVIMNLMMILKEKGITTLLVEHTMDFIKAVA
                     DWVYVLNFGQIIAKGTFDVIEKDPLVLKAYLGED"
     gene            complement(136091..137053)
                     /locus_tag="PKL93_00605"
     CDS             complement(136091..137053)
                     /locus_tag="PKL93_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ69797.1"
                     /translation="MKKTIFVIGAILLIILFPLFVKNSYHLYLMNRALIHAILATGLV
                     FLTGFAGQISLGQAGFYAIGAYSSAYFTVKLGMPIVVGVIAGIVISVIAGFLLSIPSF
                     KLKAFFLSLVTIAFGQIVWMLVINLTPITGGPYGFFGIPFYSFGGEMLSFGQVFWLFG
                     GLLLLSVFIMFRIKHSHFGRAMLAMNDDDVATETCGISTKKLKIAAFGFSAGLAGLAG
                     ALYAHLAGFLSPEPFTFFESSNFVAMAVVGGLRHMSGGVVGGIGLTLLPEFLRFGGWE
                     NYYLMVTSLIVIVIVIFMPMGLGPILESLFRKVFRVKRNNTKTL"
     gene            complement(137064..137954)
                     /locus_tag="PKL93_00610"
     CDS             complement(137064..137954)
                     /locus_tag="PKL93_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ69798.1"
                     /translation="MLQDILQNIVSGASIGATYGLVGLGVVFLWLTISRINFANISSA
                     MLAAYLFYSFYTQLNLGFFISFVLSVAIIGLYGLGLRIFIYEPIRKRGGGRLEFVVAT
                     LMLCTFWLNFIIVTYGALPKPFPPVFGGSNDFISLGGIRIPSLYLNIYLVIAILMVFI
                     YFILNKTLIGKSFRAAAQNREAAALMGIDVKVTTSLSFILATVVVGIAGILLAPIYFV
                     SLELGGGSIGVKGFASAVLGGLTNPYGTILGGISLGLLENFSTLYISSTYRDVISFSV
                     LVAVLILKPSGIFNWKRKKI"
     gene            complement(138019..139137)
                     /locus_tag="PKL93_00615"
     CDS             complement(138019..139137)
                     /locus_tag="PKL93_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ69799.1"
                     /translation="MRLNRLLVVSIVLLIGVAALGSTIKVALVAPFTGLGSILGDYIK
                     MGAQLALEEINAAGGINGDMLEMVVYDDAANPSTAANVIRRALFQDKVVAVFGPNMSS
                     SVIGVHQLAQQAKVPMLVGATSPSFRYSVIPNDFLFRLRADDKVKVIQLVKYAVEELG
                     IEKPGVIYGSTDYCLAALDVAKEEFPKYGIEIVAMEQIKEGDKDATGQLLKLKNAGFD
                     GLIGLTHESEAAVTVNQLRQLQIDVPIIGFSAWGVPAFTDLAPEAAIGVYSVQGFNPI
                     DPDPAVQAFVEAYKARWDGKEPSDPGQAYYDGMYLLADALKNVGTDGTKLAEYLANEA
                     NIVGVQGELKCDEHHNFTNVCLISQFDGKDWQIITKLY"
     gene            139549..140034
                     /locus_tag="PKL93_00620"
     CDS             139549..140034
                     /locus_tag="PKL93_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69800.1"
                     /translation="MRAVQMVCLFLFVITFSLLVAVEIDLGVSADITQSDETSLSCAR
                     LSLLASVGPLSIDLPIAQIWPSERRLELADPMLSYAGLNLKLPITVLYIKAGLGVQLK
                     GLIDLVGGSIEGWDVPARVRAGLGLSDSGLFLEGGVNLDFTPSLESLSFCPYIAAGFV
                     F"
     gene            complement(140035..140766)
                     /locus_tag="PKL93_00625"
     CDS             complement(140035..140766)
                     /locus_tag="PKL93_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ69801.1"
                     /translation="MVEEILAKRALTKSNIPLTDFTINPYVGCSIGCKYCFARFIGSF
                     KNYGGVWGRDVRVRKNLASLLKREIIRHPKWPVFLRTTCDPYQQLEKRYEITRAILRI
                     LVSHRIPVYLMTKSSLVRRDLDLLQAAEGEAKVLVTITTDREDVRKVLESGSSSFEER
                     LETIDFLRDNGIQTGAFVGPVLPMDATRVALELSKRVEEVHLDPLNYSFQVKNIFLKF
                     GWQSWLEKYTFQEVEEEFSKHLRLI"
     gene            140881..141258
                     /locus_tag="PKL93_00630"
     CDS             140881..141258
                     /locus_tag="PKL93_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNQ69802.1"
                     /translation="MLRVINTDKAPAAVGPYSQAVAAGGFLFVSGQIPLNPLTGEMVT
                     DFEEACKQSLDNLFSVVAAGGSSPDNIVKVNVYVRDMGKFSILNEIYATYFKGHKPAR
                     AVVEVSNLPKNAPVEIEAVAIIP"
     gene            141272..143218
                     /gene="thrS"
                     /locus_tag="PKL93_00635"
     CDS             141272..143218
                     /gene="thrS"
                     /locus_tag="PKL93_00635"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNQ69803.1"
                     /translation="MAISIKLPDGSIKEYESGITPAEIAKDISEGLARRAFGAIVDEE
                     LWDLERPIEKDASVKLVLDKDPESSRFFRHTMSHILAQAIMRLYGKEKVKLAIGPTIE
                     NGFYYDIDLGETRITEEDLPKIESEMNKIIKEDLPVERFELPVSEAIELMKREDQPYK
                     VQLIEDLVKDTGAEKVTFYKQGEFVDLCRGPHVSSTGKVKYFKLTSVSGAYWRGDENN
                     KMLQRVYGTAFAKKSDLEDYLRMIEEAKKRDHRKLGPALGLFTINYEYAPGMPVFLPK
                     GTEMLNQLLQFSREKHVEDGYREVSTPQIMSDALWRTSGHWDHYRDNMYFTEKEDQQF
                     AVKPMNCPGHIIIYKSSSVSYRDLPMKLFEFGKVHRYERSGVLHGLFRVRGFVQDDAH
                     IFCTREQIQQEIMGVIDFVQKIYSPFNFEYRAELSTRPEDYMGEVELWDIATKALEDS
                     LVAKKMEFKVNEGDGAFYGPKIDYHIKDSLGREWQCATIQLDFMMPERFGIKYVGPDN
                     EEYQPVMIHRAIFGSVERFMGILIEHFAGAFPAWLAPTQVSVIPIADRHAEYAESVAA
                     ELKSRGFRVEVDDRQKSTNYKIREAQLMKVPYMIIVGDREKEDGTISVRLRSEKDLGS
                     IALQEFMDKLSQEVNMRSATSIFE"
     gene            143306..145030
                     /gene="ade"
                     /locus_tag="PKL93_00640"
     CDS             143306..145030
                     /gene="ade"
                     /locus_tag="PKL93_00640"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNQ69804.1"
                     /translation="MPVARGDRPADVLLKNCKIVNVFSGEIEEANLALFKKRIAGIGD
                     YEDGLEVIDLEGSYVVPGLIDAHLHIESSMVSPVEFSKTILAKGTTTAIADPHEIANV
                     MGLSGIEYMIKSTEGVPVNLYIMIPSAVPASALETSGARISVMDMIGFVEKFQNRVLG
                     LGEVMNFPDIINGDRDSIAKIELIRHKYKKIDGHIPGVLGKPLNAYICAFVRSEHECT
                     YVEEAREKLSRGMQILIREGSVERNLVALLPLINEKTYPFVSFCTDDKHPDDIIREGH
                     IDYNIRKAIEHGIDPIIAIRAATINTARHYNLRSMGAIAPGYKADLVVVDNLESFNPR
                     IVFKDSRIVARDGRLVIDVISKSFPQERTNTFKCQKICEDDLRVKAEGRKIRVIELLG
                     SEVLTGGLITDAKIENGCVVSDVDSDVLKLASVCRYCEGKSMSVAFAKGSGLKKGAVA
                     TSVGHDSHNLGVLGVNDSDMVCAANRVIEMGGGLVSVVDGKITSELPLKIAGLMSDLT
                     SREVAERLTELKEATKIMGSTLPDLFMTLSFVQLSVIPKLKLTNLGLVDVEKNDFVAL
                     FVKEGEDA"
     gene            145023..147329
                     /locus_tag="PKL93_00645"
     CDS             145023..147329
                     /locus_tag="PKL93_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNQ69805.1"
                     /translation="MPERHEKVVGKKINRVDAREKVSGRAKFAADVSFDRQVNCALVI
                     AKKAHGVLKSIDASSAKRIPGVEGVFTWRDVPGENQLGDVIKDMPCLVKEGERVRFYG
                     DVVAVVAASSKKIAEEAAELVNVEIEELKPVLTIEEAIKDEIVIHGKSNIGVSRRIRK
                     GNASKFFENSEHIFEGEFYAHYQEQAYMETQGVIVVPDIDYGFTVYGTMQCPYYVQGA
                     VSNVLGIPMSRVRVIQTETGGAFGGKEDIPSYVASYAAIVAYNLRRPAKLIYSREIDI
                     QTTSKRHPIKSHYKVALEGKKIKAMTIKAYMDMGAYATLTPIVIFRTLVHAAGAYEIE
                     NVDVDVYAVYTNKVPPGAFRGFGSPQVLFAVESMLDDIAVQMGFDPMEFREINTLRPG
                     SRTSTDHLLVNSVGAPETLSRVKKASKWKELVKETEEFNKSSEEVKRGVGLSHIFYGV
                     SLGAGGQALDKSGAHVLIDRDGSVEVRIGGTEMGQGAKTVISIIAAEELGQSIDKIVV
                     HQPDTAFVPDSGPTVASRTTIYSGNAVKNACASLREKLAPVFCELTGSPLSSVRFEEG
                     FVYGESGKKLSFDEVVEHAYKRNVKLFETGWYETPKLKFDMDNGIGEAYVTYSYASQV
                     VQVEVDLATGYSKVIKAFTAHDVGRSVNPEGVIGQIQGGFAQGMGYAIFEDMKQKDGK
                     IISDNFNTYIIPTVHELPEELVIDVVEDPFPEGPYGAKGIGEPSLMPVPAAVANAISM
                     AIGRRVRRIPVTPEYVLQLLENGSSLQE"
     gene            147355..148740
                     /locus_tag="PKL93_00650"
     CDS             147355..148740
                     /locus_tag="PKL93_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNQ69806.1"
                     /translation="MGKFVLGPTFEEMLHPEKIDRKTREIAWKMRKEDPLDPINLYNI
                     SWRDIDNRIYYFVLPKEFTGVDANIVVLYGKDFPTGSHKVGATYSILMEKVLRGEVDP
                     TYHTLVWPSTGNYGIGGAWVGCRMKFDSVVILPEEMSAERFNIIREYGARVFATPGCE
                     SNVKEIYDKAKELKAANPEKVRVLNQFDELGNYRFHYYVTGNTILEMMDELRLGNGRV
                     SAVVATVGSAGTIACGDRIKEVYPEVKIVAGEPVQCPTLSLNGYGGHDIQGIGDKHVT
                     WIHNVMNNDAVVALDDIECKKLLQVMTDEEGKKFLKEFLKADDVDTMADIFGISGVAN
                     IVGAIKMAKYYRMSDKDNIVTIATDNIDRYHSVMENMSKTYGQLTRDEARHRFESYVL
                     GAKTDWIFEGTMDNRLRWHNLKYYTWVEQQGRSVEELNKTMSQDFWKAESGKIEEIDK
                     LLLDYRVRNKE"
     gene            148899..150218
                     /locus_tag="PKL93_00655"
     CDS             148899..150218
                     /locus_tag="PKL93_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ69807.1"
                     /translation="MILLKKFLLVSVVLMFSLILSATTIQFWHAMGGWRIDFLQSQAD
                     AFMELHPDIEVEVQYTGSYRDTLNKMIAAVQAGTAPHVVQVFEIGTQMMIDGGVAIPI
                     EEMMEADPTFDIGKFMPQVLNYYRVNGKLYSMPFNSSNAIVFYNKSLFEKAGLDPHNP
                     PTTFEDFIEVARKLTIKDSAGNVEQAGFTVSLNSWFFEQLLAAQDAPLIDQNNGRTGR
                     PTKAVFNTPEGLAIFEWWNTMSQEGTMINTKFEDWTAARNLFISQKAAMLMTSTSDVA
                     LMMESAKTNGFELGSVFLPRPSDSKAGGAIIGGGSLWLIDGHPDEETKAAWEFIKFMA
                     STEQQIEWHKATGYFPVRKDSVEKLVASGYYGDYPDHLTALLQLLLSVQSYNTNGAIT
                     GAFPEIRTVVTTAIEKMIAKEMTPSEALKYAEDGATQAIRDYNEANY"
     gene            150279..151145
                     /locus_tag="PKL93_00660"
     CDS             150279..151145
                     /locus_tag="PKL93_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ69808.1"
                     /translation="MRKWLPYLLLVPTFLIIVLFIYYPAVEAFRMSLYRVSTFGNKTT
                     FVGIQNYINLFNNEQYLSSVKLTVIYVGLSVTLSIFLGFIIAMLLTQNVPGTKLYRTF
                     LFAPYAISPAIAGTLWTFLLNPVVGHVNYVFMKLFGLQVEWLTSKPYAFYALIFATVW
                     KILPFNMIFYIASIQNVSGDLLEAATLDGAGVMKKTWRIIFPLVSPITFYLVIMNIVS
                     TMFTSFAIVDVMTKGGPGGYTTNMIYRLYLDAFTYQKRGPASAQSVIMFMIMVVVTIV
                     YFTVAERRVHYQ"
     gene            151155..151994
                     /locus_tag="PKL93_00665"
     CDS             151155..151994
                     /locus_tag="PKL93_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ69809.1"
                     /translation="MKKSTRKLVNTIIIEIILITVSFIFIMPVILAITMSLQPPQSVF
                     SFPPKFFPTAFHIQNYFDAFKTVPFARLLLNSAIVSLAITLGKLVTGTLAGYAFSNFR
                     FAGRGLAFGFLFATLFLPAETIMIVPLFSLMNKFGWVNTYWALTIPFMASATNTFLMR
                     QQFMTIPLSLEDAARIDGAGPIKYFLKILLPLSKSIIAGSAIINFVYSWNIYLWPLIV
                     TMEDKMKTVQIGVKMLIDAESANNWGIIMAGTMVAVIPTLVVFFLLQNLFVKSLVGTG
                     LKE"
     gene            complement(152144..153988)
                     /locus_tag="PKL93_00670"
     CDS             complement(152144..153988)
                     /locus_tag="PKL93_00670"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNQ69810.1"
                     /translation="MKKTYQWTTKSITIIVVLILLKSFVFYGYAAELARLSVLLGTVA
                     WMAFLLLLFWIVFKGKFFLLYCIISCILFVDFLYFQYFGFLPSVKELVHVGHVGAISE
                     SIVYVLYPISFVFIIDLIPVGLYLRKKQSRQGETPIRKPTYSDRIITFVLLISLLIPF
                     SSESLQSYFVFNRYGVFAYHMFDLVRAIPGVDGGITVDEKVSYSGYVNKMAERGKYFS
                     VAKDRNIIVIQLESFQDFLVNFVYNDQEITPNLNRLVSSDSIYFDEIYQQVGAGNTSD
                     CEFVILNSMHALGDVSVYQTREENSFYALPSLLKSKGYFPVAFHGNNGWFWNREKIYP
                     SLGFDDFISLEDMENDEIIGFGLSDSSLFKQTLGHLRGIEEPFFAFIVTLTSHNPYEI
                     PEELRAIELLPEHEGTLFGNYIQSVHYTDAALGEFIEGLKEAGLYDSSLIALYGDHAG
                     LYPFNKENKDILSELLACEYDFVHAMNIPLLFHIPNSNLDVLNSKPGGQIDFFPTLLN
                     LIGIVEKSGILFGRDLLNSSSGFAAFTHYVPEGSFIDNNRIFIMSSDGILDNSEAVER
                     RTGKMITPYSCLDGYKDSIQQIQASKYLILNDSISNLMGVSNERDNRF"
     gene            154489..154776
                     /locus_tag="PKL93_00675"
     CDS             154489..154776
                     /locus_tag="PKL93_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69811.1"
                     /translation="MAKDRHRNFFLSNVMAITENSPVGLCFEDNYSGSVFLNRGARES
                     GLRVSDSVSYHGGESFVEFVKSENFERCFRSMVIDYDEKVSGLDVRNSLLK"
     gene            154962..155387
                     /locus_tag="PKL93_00680"
     CDS             154962..155387
                     /locus_tag="PKL93_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69812.1"
                     /translation="MLREKPSQFLTTVKSVTEKSREKAAGLYEKGRRQSFQILRRAGR
                     KNREVANTDRLSANLLEDTGVFQTISSGPDEFSSEAIELAEVLIVHAAEAINRIRNIF
                     EMTRPTFNDPLTGALGRYGLKKFSRAETERAKRNLSDFN"
     gene            complement(155757..156185)
                     /locus_tag="PKL93_00685"
     CDS             complement(155757..156185)
                     /locus_tag="PKL93_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69813.1"
                     /translation="MEDYRWIYLAILLQAALLGTVLFLGDTLFHSSVESEFVKEITAK
                     EIGSSLLGDYLKGFEDRWLPEESRLTGYLIEDVIVLEETDNYTVLLASISVKPTDIDS
                     CLWNSLGSREGSWIKDIRLSVYLERDQTGKFTIVKTVPAI"
     gene            complement(156428..156931)
                     /locus_tag="PKL93_00690"
     CDS             complement(156428..156931)
                     /locus_tag="PKL93_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNQ69814.1"
                     /translation="MSVENGRNVLVVYYSHDESTKSIAESIAGETNADLLELKPVEKS
                     GGRKVKYIWEGESVSMDPVPDLEPFLLDPDNYDLIFIGSPVWAMSYAPPIESFIRKTK
                     LSNKNIALFCTHEGLMGIVFQELISKLSENKIVGAIDFYDPIGSGVKYAANASANWAR
                     QVLKSLS"
     gene            complement(157025..157495)
                     /locus_tag="PKL93_00695"
     CDS             complement(157025..157495)
                     /locus_tag="PKL93_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ69815.1"
                     /translation="MKLLTKNEKITIDRFENMETADKEIGIDRIVDFLHTHLEEYGDD
                     RKAIWRSIEYAFSNEAGKGGFVLIARHGERILGASVVNDTGMKGYIPEHILVYIAVDR
                     EARGIGIGKKLIKQIKRMCNGDIALHVEENNPARFLYKKMGFKTKYAEMRFAQD"
     gene            complement(157921..158067)
                     /locus_tag="PKL93_00700"
     CDS             complement(157921..158067)
                     /locus_tag="PKL93_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69816.1"
                     /translation="MRNHRSGLREKARQAKTFLLFHEMMEPSVIKEDRIVDVRGNAAT
                     VDEQ"
     gene            complement(158100..158300)
                     /locus_tag="PKL93_00705"
     CDS             complement(158100..158300)
                     /locus_tag="PKL93_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69817.1"
                     /translation="MSFKKGEVFGNPDEVLIDAEMAAKEHRSRNRVSPFHSILMHERN
                     YTGLSEHQQTMIAYSCTSLGNK"
     gene            complement(158567..159490)
                     /locus_tag="PKL93_00710"
     CDS             complement(158567..159490)
                     /locus_tag="PKL93_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ69818.1"
                     /translation="MIIQGYSKDFKQRILDIQLDYERDHPDAEKFTADDFRNPLLNGR
                     KNILLAVDNGRVLGFSRFFSRQNFSREFFHHIWLEIILEHFADPDLMSALYKASIPLI
                     IESTRGFFPSAGRRICVKVADTEKTKSDFFSSAGFTHWIDFIYMEKDLSEEISSYSLP
                     EGLNIAELKPRREPEILQYLRVEQICFPDSPLEYKYLNYFFSRDEWRREGVIIAAIDA
                     GGNIAGSVMVYPDAQNSAKCYTEEIFVVQEWRGKGVARALLATSLTYLKKLGKTRAIL
                     SVTSDRTSAKKIYEKAGFKVRLKRRVLALEI"
     gene            159758..160399
                     /locus_tag="PKL93_00715"
     CDS             159758..160399
                     /locus_tag="PKL93_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HNQ69819.1"
                     /translation="MFFGKKEVAIIQLFHEHLDFISKTLENLEKVFLAIESENSMSIE
                     IYAEEVGKMESAADAKRREMENAMYQGAFLPNFRGDFLGLAETFDKVANEAENVVDQI
                     ILQHLSVPQELRNDLLKQAQLAIKTYEVSKEAALNLFQELSVAEEMIKETERLENIED
                     SHERALIKRIFEMDLTLAEKRQLRELVLSIGDIANYSEDCSDRMEIIVLKRRV"
     gene            complement(160400..161335)
                     /locus_tag="PKL93_00720"
     CDS             complement(160400..161335)
                     /locus_tag="PKL93_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNQ69820.1"
                     /translation="MLFAIIPAVVFGWALGANDAANVYGTAVTSGLVKYRVAVVLSAV
                     FIVIGALLEGSRGLETISSVSSQTLLTASVSTIGAAISMTIMTYLGIPSSSSQAMMGA
                     ILGIGILNSTVDWSVLIKVVICWIATPIGAAIGSFFLYKLSAILFRRIKTIQAQDLSL
                     KIGALIIGAYGSYALGANNVANVTGPLAGIISYDIAALVGGLSIALGVLTFSKRVMLT
                     VGKQITLLDHFSAVIAVLSQAITVWVFAVIGVPVSTSQAIVGAVIGAGLARGSSDINF
                     KVLRNIALGWLQTPLIAGFVSLGLYLVVNAVKGLF"
     gene            complement(161472..162239)
                     /locus_tag="PKL93_00725"
     CDS             complement(161472..162239)
                     /locus_tag="PKL93_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNQ69821.1"
                     /translation="MKHWKFWLIFACVVAYVFLIEPNILTVERLTFSDEPSAKIVFFA
                     DIHIWTKKPLHNRLLEKFTDEGVDLILFGGDVLAPYTDMEYMESFLSKLTAIAPVYAV
                     YGNWEESETTTMQKIYEEAGINVVHANSTEIQISDKKLGLTGTPSHHYFSWTKFLPED
                     DYDLKILMVHAPNLLEEHENVLKDFDLVLAGHTHGGQFYIPWLTEAILRSTRGFSGDF
                     FRGLYQINGTKVFVTRGVGGWFPGRLASLPEIVFIEF"
     gene            complement(162314..163471)
                     /locus_tag="PKL93_00730"
     CDS             complement(162314..163471)
                     /locus_tag="PKL93_00730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ69822.1"
                     /translation="MGFLQSQSISISVFLGIMLVITLSNLFVIKRMSRLKKAIPGPMV
                     SILIPARNEEKNIFACVTSLLNQDYSNIEVIVLEDCSSDRTMEILQSLAKNSDNLRII
                     KGKALPKGWLGKHWACNQLSMEAKGEILFFTDADTVHSPSTVSFAVKAMQIEDLDLLS
                     AVVKERTDTLGELLTVPFMVHSILSIFPLALAYRTKFRALSVTCGQFMMFKKESYCSI
                     GGHESVRDHGVDDLSLGKLIKKAGMRWRLYNASDLVECKMYESFKDACAGFLKNYFAL
                     FEYRLIPAIFVWTWILTINLFPLVMLILSISGTVTSPTIAISSTVSVLFSIIFWFLAS
                     EKFGLSPLVVVLYPLIATISSIIGYMSIIFHFFGCTKWKGRALVKKKSRFI"
     gene            complement(163473..164060)
                     /locus_tag="PKL93_00735"
     CDS             complement(163473..164060)
                     /locus_tag="PKL93_00735"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNQ69823.1"
                     /translation="MFLLWAALGFLSGSVMYSKILYEKTTGKRISEIADGNPGSSNVI
                     RGAGIILGLIAMALDYSKGYLPVLLARTVGDISGLGLVPVAISPVLGHAFSPFLGMKG
                     GKAVAVSFGIWSGLTLWVGPTIIGVLMLFFTFILVPSTDGWKVILSMFGLLALLLVQR
                     DLVLTLTWILNTSLLAFKHRDQLVFPIKLKFFGGK"
     gene            164268..165497
                     /locus_tag="PKL93_00740"
     CDS             164268..165497
                     /locus_tag="PKL93_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ69824.1"
                     /translation="MKRIQFIGIILIMWTTAFSVQNLADLSSEDWREDIEYLEERLIS
                     IHPNPFFRIDREVFQASIKALEQGLDELSWYEIYTRLTEIVASIGDGHTVVYPQGSST
                     LYPIYTYWFSDGLYVIATSESEQRLLNSKVLEISGTKVDDALKALRRVVSHDNEWGFM
                     NSLSDYLNKPEIMMGLGLADKEGNLVLKIENNGAVSEVLVKPQNAGFQWVQKRIEEID
                     RTYIGRKYWYQYYPDSRILHFHYASCGSEERNPFILFNCRMFLFTWTNPVDKFVLDLR
                     GNGGGSSVILEPFILRMMIDWRLNRTGKFFVLIDRGTFSSAVLNAISLKKRTNAIFVG
                     EPTGGSPRHYGEVKRFVLPNSGISVSCSSTYWRTTSDTSPAFMPDVIAIRSFEDYYYM
                     RDLAMEAVESYRTEKEE"
     gene            165498..166616
                     /gene="iadA"
                     /locus_tag="PKL93_00745"
     CDS             165498..166616
                     /gene="iadA"
                     /locus_tag="PKL93_00745"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HNQ69825.1"
                     /translation="MITLFKNVEVFTPKPLGKKDILVSGERIIEIDERIDESAVVSLN
                     GRVLDLSDRYAVPGFVDGHVHLIGGGGEGGFSTRTPEGSSKQFLEVGTTTIVGLLGTD
                     GITRDHISLLAKARDVQNDGLNAFMLTGSYRYPLKTITGDIMRDIVLIPEIIGVGEVA
                     ISDHRSSNLSAKELQRFAMDARVAGMLSGKAGVVVLHLGDGQEKLKPLYEATSDGTLS
                     PTQLIPTHICRTEELLSQGIDWVSNKGGYIDITANEDNTHLVLSDIYRRKIRFDRICV
                     SSDGLGSLPKFDSEKNLVGIKPAPVDTLMKSFIKLISEEGLPIDEALRPFTINPSRFF
                     HLEENGIGTLKKGGIASIVFLHRDLSIDHVLSNGRLLV"
     gene            166633..167130
                     /locus_tag="PKL93_00750"
     CDS             166633..167130
                     /locus_tag="PKL93_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNQ69826.1"
                     /translation="MEFAAVYYSRSGSTRAIAESFASSCGAKLFKLEDLKPRKSVSGF
                     SALLGLGSALKEPLPDVRSFDLVVLFTPIFAWHPSPQMNTFVRNGDLKGKHVYLVGVG
                     AGESNVKALERFKKEVERAGAKVLGSKNLKGLQIKQNIKEVEPQLLESGNQLVGAIEK
                     AFKET"
     gene            complement(167127..167720)
                     /locus_tag="PKL93_00755"
     CDS             complement(167127..167720)
                     /locus_tag="PKL93_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNQ69827.1"
                     /translation="MTICYYLVNLTTGALDGESVSCYGLALDAFAVSICTGISLPNVT
                     GRHVFRVSFHFGFFQFGMPLIGVFLAAGFRDFIVDFDHWIAFILLAAIGGEMLYESFE
                     NQGEETCKDRTKGLTLILLSVATSIDALAVGSAYAFLEKPVIETAIMTGVITASLSAI
                     GVTGGQRIGQKLGRAMEAAGGTVLIAIGAKILLEHLF"
     gene            167929..168552
                     /locus_tag="PKL93_00760"
     CDS             167929..168552
                     /locus_tag="PKL93_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNQ69828.1"
                     /translation="MEIIYGTRNLAKVMSMKKMLCGLDLQLKDLSFFEHLPVVEEIGS
                     SPLDNARAKAETYYRFFASPVFACDSGLYIEGLDSGLQPGAKVRRKGSKVLNDDEMID
                     YYKKLLEGLGGQAVARYRNAIFLIFDDDQKCWLMDDRIEETFYLVSRPHPSRVEGFPL
                     DSLSIDIRSGKYFVDLTDEERSSAYHCRDGFRNFFSEAISSFSDRHF"
     gene            168621..169448
                     /locus_tag="PKL93_00765"
     CDS             168621..169448
                     /locus_tag="PKL93_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ69829.1"
                     /translation="MAYRTINGVKLYYEIRGNEEGEEAVAFFNGVMASVNSWSLQARV
                     FERFGYKVLLHDFKGQLLSEKPEGPYTFKEHAEEARLLMKELGIEKVHLIGTSYGGEV
                     ALRFAIDFPDTVKSISIIDSVSELDEVLKLFIQGWKDAARAGDPRKFFWDMVPTIYGD
                     AFIRKNLKMLEERAMALEKVSSDYFEGQIALYETFLGDVYMTDLLERISCRTLVVCGE
                     EDILKPRKFSKIIADRIQKSEFAIIPDSGHVTIFEKPDVLNSLLLGFVMKSSDLYAQ"
     gene            complement(169791..170774)
                     /locus_tag="PKL93_00770"
     CDS             complement(169791..170774)
                     /locus_tag="PKL93_00770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ69830.1"
                     /translation="MSKIVTLRRYVPERDYMQIRELIIETFPLHGGPFNWLIERWNFC
                     RFHVLPLHRYYSTSYFSVPTRPHIDHRDDLPLWQDSIGIWENEEGRIVAVAHSENEEP
                     GEAWIQIHPAYKYLYPEIVDYIEENLADRANGIGFVKLYVNDGDELEKIAKSRGYVRQ
                     ERCIPHLVYEIGESMATPSFPEGFRVLSVAEEDDPVKRSRAKAMAFSGYCPPSSWPPV
                     DAFREMQMAPDYRKELDLFVVAPDGEYASFCTIWLDLKNGYGNFEPVGTHISYRKLGL
                     ATELLKEGFRRMKSFGIDRSFMNSTVGFYRRFGFKETSRYCRPWIKYFKIQ"
     gene            complement(170944..171348)
                     /locus_tag="PKL93_00775"
     CDS             complement(170944..171348)
                     /locus_tag="PKL93_00775"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNQ69831.1"
                     /translation="MAKLTIVEGIGPVYEKKLKEAGITSIESLLKTCETKKARTELAK
                     KADISEDNILTWVNHADLMRIKGIGGEYSELLEAAGVDTVPELSKRNGDNLFQKIVEV
                     NGEKKLVRKLPTKKQILNWIEQAKKLPRAIQY"
     gene            complement(171428..172033)
                     /locus_tag="PKL93_00780"
     CDS             complement(171428..172033)
                     /locus_tag="PKL93_00780"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNQ69832.1"
                     /translation="MLQNVQRRRPINLFDLISQKLNDEDTSSELAKSVGTTPDKVQKV
                     TQLGLPTLLQGLTRNASTQEGAESLNRALDQHKDENVDDVKSYLSNADRSGGQKILSH
                     IFPENEERVEKNLATQTGLKKNQVSGILSQIAPLLLAALGNEKTKSNVDSSGIAGFLS
                     NIASSSSNSGMMGKAADLLDKDRDGSFLDDAGSFFGKFFKK"
     gene            complement(173011..173262)
                     /locus_tag="PKL93_00785"
     CDS             complement(173011..173262)
                     /locus_tag="PKL93_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69833.1"
                     /translation="MASTLTYVRRAAVQRKEERSAYLLKSASDFVDEDGDRVNELFPN
                     EGQTLWHGLRDGNLKRMNRPTKTRVIKETSPIAEAGVQF"
     gene            173998..174948
                     /locus_tag="PKL93_00790"
     CDS             173998..174948
                     /locus_tag="PKL93_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ69834.1"
                     /translation="MKRATLREVAKKAGTSMMTVSRVINSKDSVSDETRNRVLRAISE
                     LGYEPHKDARILRGGKTGRIGIVVSDIRNPFYSQVVGDLEDLAEENNMAIIVSDTSKK
                     LNQERKAIKSLLDIKVDAILVAPEGYESSHLIDVINSGTDVVSFGVHFENDEISEVSI
                     DEITGAAKAGAYLKSVGIKDVVMMMGNPRKFTTRGRMEGFMRGFGHVPEDRVIYCEVD
                     WKASCATVSEMQKLPQAFFCYNDMMALGVMKALEERGIELGSEVKVIGYDDVYMAEIA
                     GITTLRIPIRSMVKEAFKTILGDEVRKMVFTPEFIRRKSA"
     gene            174965..176182
                     /locus_tag="PKL93_00795"
     CDS             174965..176182
                     /locus_tag="PKL93_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ69835.1"
                     /translation="MKRVLLVILFVSVALVGLGIQQLVVNSYMSDPEPKRVFEELVQQ
                     FESMYPDFEVTVNTFAHEDFKVLLRTWLTSSNPPDVVTWFAGERMRYFASKDLLEPID
                     DIFENGFEADFPKAFVSASSYEDRIYFLPQSWYWWGVYYRKSVFEELGITVPLTWKQF
                     LDVCETLKANDMIPITIGTKYLWTAAGVFDYLNMRVNGIDYALKLTNGEIPYTDDGMK
                     AVFEYWKQLVENGYFIEDATSYTWQEAATYLFTGEAGMYLMGQFIKDVAPPDVKDDLD
                     FFRFPIIDGNLGLYEDTPIDGFMMPANAKNKEAGKTFLKFIASKEAQEYFATELGRLA
                     ANKHVPAPDDHAKKGLEMILSSDGVMQFYDRDTDPEMANAGMNGFVEFMTFPSRLDTI
                     LKNLEAERNRIYK"
     gene            176236..177108
                     /locus_tag="PKL93_00800"
     CDS             176236..177108
                     /locus_tag="PKL93_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ69836.1"
                     /translation="MRSKRWVPWAFLALPLTMYSIWVIYPLISTLLLSFTSWDGVSMS
                     KDIIGLDNFRELFRDPYFIVSLINNIKWLIGFAVVCVPAGLGIALLLDQNFRGNKLYK
                     TLVYLPMTLSFVVIGQIWSWILEPRSGVLNSLLALLGFKGVSWLSDPSVVTYSLIMAA
                     SWRQISYAMVLFLAGLKGVPRELVEAATVDGAGPWKRFWNVVLPMLKPATVVAVTVSV
                     IDSLRAFDIVYVLTRGGPFYSSSVMANYMYIKAFNNYRMGYGSSIATIQFLITLVFIV
                     VYMRNVLKKEVGNE"
     gene            177105..177923
                     /locus_tag="PKL93_00805"
     CDS             177105..177923
                     /locus_tag="PKL93_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ69837.1"
                     /translation="MKKTLFYIFATLLVLVWLMPFVITMFTSVKSMDELMMGRRWWEP
                     PKELRFENYATAWQDANMGRYFMNTFIITVPSVLGALFLSSLGAFALAWYDFRLSKTI
                     LMIFVGGMLIPFQMLLIPVYRMSISFGIYDSYIGVILFHIAFQLGFCTFFLRNFMKTI
                     PASIFDAAMIDGAGHFLIYRRIVLPLVVPAMAALGILEFTWIWNDYLWSLILIQSDRF
                     KPVTLGLVNLQGQWITSWNVMAAGSIIAAVVPLVVFLLFQRYFIEGLTVGSVKG"
     gene            177920..179803
                     /locus_tag="PKL93_00810"
     CDS             177920..179803
                     /locus_tag="PKL93_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNQ69838.1"
                     /translation="MNWFGAAYYPEHWPRERWKKDVRTMGEIGINVVRIGEFSWSVVE
                     RKRGEIDFSVLDEAVDLLDSEGIKVIMGTPTCTPPPWLTKEFPEILQKDVNGLVIASG
                     SRRHYCFNSKIYREETSRIVEAFADHFGNDPRIVAWQIDNEFGCHNSTVCFCEDCAVA
                     FRVWLKKKYGSIDKLNKAWGTVFWSQIFNDWEEIEPPRRTLTSPNPSLVLDYKRFSSD
                     SAIDYHNLQREIIMRKSEAPITHNLMVNFTEIDYKKLSDSIDFVSWDNYIVGDYDPDL
                     QALNHDLMRSLKKQPFLVMEQQPGRVNWRTVNPSYESSQLSFWVKQSFAHGAFGSLIF
                     RFRQLPFGSEQFHTGLIDYDGEPTERAQAFSKAIEKLTDWSVVVPQREAAIYIDYENF
                     WINDTDNLNNKFDLLIDGILPVYKSVRSLGYNVDFVFPGDSLEGYLLAFVPSSFKLER
                     CFVEELRRFKGDIFFTALTDQKDGRNFIRKTEESFITELMGLRVVDAGGIETKVEISF
                     ADHVFNGSFVVEKILPSEDCEVLAVYLNGPFTNHPALIKRANMYYLSTVPEIVSFQTL
                     LIEAGIERKASGPGRVIKGESNTILQNPFPHSITMEVGGKDYTLNEYEVRRVQTGLSK
                     NNR"
     gene            complement(181230..>182041)
                     /locus_tag="PKL93_00815"
     CDS             complement(181230..>182041)
                     /locus_tag="PKL93_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69839.1"
                     /translation="RWKYSKEGTENIVEFSGNYLDENGEAATVKMRFLVKEEDSTFIM
                     NYWEINGVPQDNSNQFAFLEKIFLPERADVAISVVKEGYFYDYPDKIIGESFSTYFSD
                     GSWDYFVTADNQDVVEYIGFLYEEDETIETVFQFTVNLDERTFEIAYLGIDDVSQDDE
                     AIDTLLASILDSKITEVKNSYFDVYYNWMTLGEAFDGFFYEPHWDFFVSTDDLNIVEF
                     YGTFDLGGIPAEVYAQFEVYEDGTFDLYYWEIDEQYETINAFYLLLELIYY"
     assembly_gap    182042..182141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<182142..182371)
                     /locus_tag="PKL93_00820"
     CDS             complement(<182142..182371)
                     /locus_tag="PKL93_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69840.1"
                     /translation="MKKLTILLLLVLSVSSIALAQRLLPQDSARFISIVTEGTFNNFP
                     DNLIGESFEAFFEMPRWKYSKEGTENIVEFSG"
     gene            complement(182435..183358)
                     /locus_tag="PKL93_00825"
     CDS             complement(182435..183358)
                     /locus_tag="PKL93_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNQ69841.1"
                     /translation="MKNFVLLLLALLVSVPTFGSSVVFEKSWGVQGTKNGQFSDPQGI
                     FVDGKDLVYVADTLNHRIQVFDKNGKHKTSFGSYGSENGEFLFPHDIVVDSKGFIYVA
                     DSQNGRVQKFSSKNKFVKSWGSKGPEPGQFDGPMYLAVDKSDRIYVGDAFNQRVQVFD
                     NEGTLILTVGAKVGMFDAMNPGNLATVSGLSVDNNGYIYISDDIVRRIQKYDPEGNFV
                     AEWGSGYSGKWGQPGGSTIDSSGNLHYINKMNNMVVVLDPKGSFLFEWGGSGTASGLF
                     TRPIDLYFSDDGSVFVLEQSGNRIQRFGISY"
     gene            183714..184847
                     /locus_tag="PKL93_00830"
     CDS             183714..184847
                     /locus_tag="PKL93_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNQ69842.1"
                     /translation="MIKKNYLLAPGPTPVPIDVLLEGAKDTIHHRTPQFKKIFENAVN
                     GTKEVFRTQNDLFILASSGTGAMEMAVANIVNPGEKLIVCSVGKFGERWVELAKTFGA
                     EIVLVERDYGDFYTPEMVERALDENPDAVAVLTTLSETSTGTVMDIEGISKVVKKAGK
                     LIAVDGISGLVAERLLTDDWGLDIVVSGSQKGFMLPPGLAFISFSKEAIEKSKNVSST
                     SFYFNLKKYLKDPIPWTPAVNLIYQQSLAVKMLLEEGMENVWARHELMGKATREAIKA
                     MGLELFSKRPGNVLTSVKVPEGVDGGKIVSIMRDEYGVTIAGGQGSMKGNIFRIAHLG
                     YMSDYDVIIALTSLEKVLRRLGFKVEYGTGARVAMEIFEKEGA"
     gene            184847..185770
                     /locus_tag="PKL93_00835"
     CDS             184847..185770
                     /locus_tag="PKL93_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNQ69843.1"
                     /translation="MLRLHANDPLDKDAMKILEDSKLFEITAEHLDKDEMLKKMPEIE
                     ILVVRSATKVTSEVIDAGKKLKLIARAGVGLDNVDVEAAKRHNIMVRNTPGANAISVA
                     ELTFGLLLSLVRHIPRGTYGIKEGKWEKKELKGTEIFGKTIGLIGFGAIGREVAKRAI
                     AFGMNVCAYDPFVKETEMEIELVSSLDSLFDKSDVISLHIPLTAETKHIVGEKEISAM
                     KDGVIIINTSRGGTIDEQALYNGLVSGKVSGAALDVFEVEPPSDELRRKLIGLGNVIC
                     VPHVGASTTEGQKRVGLEMAKIIVEECKSMI"
     gene            185803..185982
                     /locus_tag="PKL93_00840"
     CDS             185803..185982
                     /locus_tag="PKL93_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNQ69844.1"
                     /translation="MKVYVDKDTCIGCGVCEGICPEVFKMNDDGKAEVIVPETEAACA
                     QDAADSCPVQAIKVE"
     gene            186176..187519
                     /gene="dnaA"
                     /locus_tag="PKL93_00845"
     CDS             186176..187519
                     /gene="dnaA"
                     /locus_tag="PKL93_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNQ69845.1"
                     /translation="MSNSIISTLKKKVSKKTWDNWFSTFELKAVEDERVVFSVANLFI
                     KDWLQTKYGAVISDSIAEILGRRVSFEIVYKNKETPSVDSTSDQISGSLLKRKPLMIS
                     NLNPEYTFSNFVVGSENKALYEVALDVTQNPGKYNPFFVYGGVGLGKTHLLQAIAQET
                     MSNFPDKKVLYITSEQFMNDMIQSIKENNIQKFRDHYRKKSDILLIDDIQFLIGKKGV
                     QNEFFHSFNELHDSGKQLIICSDRNPEELETFHSRLKSRFQMGMLMSIQEPRPSTRFH
                     IAKQLAQRESVSLPDDVAKVLADNIDGNLRRLRGAIIKLIVHSSVFRSQIDLSLATQI
                     LQSFTGSINVPAFQRPIDQIYSAIEKTMKVTKKEIESGSRSKDIVLARQLTMYILKNH
                     FGKQVTEIARETGKQHSTVIHSLKKIDKSVMMGKGATKLLYDDVIGIITSNSAAV"
     gene            187673..187870
                     /locus_tag="PKL93_00850"
     CDS             187673..187870
                     /locus_tag="PKL93_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69846.1"
                     /translation="MKYAVSFLLFLVSAATIIGTISVSQASRLRESMNEKSGAFPNMV
                     DGVFAVSEGCISLEVIDSSRE"
     gene            complement(188166..188645)
                     /locus_tag="PKL93_00855"
     CDS             complement(188166..188645)
                     /locus_tag="PKL93_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69847.1"
                     /translation="MSRRNFIFLLLSLISLSILTSGCINYVLARPESVLTKLEREINR
                     AKEDRIIELYSEEVWVATPGNPSGRILPKHSLASFYSDFFELYEDISIELHSEETLRD
                     SLEATMTVSFTLEYSYEGKRYYDIGEGSILLSGIDNHWQICGERNNPILSLFEPAEM"
     gene            188834..189820
                     /locus_tag="PKL93_00860"
     CDS             188834..189820
                     /locus_tag="PKL93_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HNQ69848.1"
                     /translation="MRMNIAYLFLVILTTVVLSATFLNFKDDYSNIRVQTSDVRRGES
                     FSISVPKSTYERSTLSYVRGATLESFYSDSDLVTYSFCADDKNAVIVVDFKGLFRASK
                     TLSLEFNDFVDSNSDGFPDKLVLDCEDAENFRAWFVNIAAYQVIEFSNNWKSEERDCS
                     GLIRFAAREALKTHDEEWFSETAVDYDLWREKTGIDLRKIPDVREYNYPDIPILRNRI
                     FISNDGAFTYFADAYNLVRSSMVFKGRDLGAARPGDIIFFHHPSPSTFHSMIFTGDGL
                     IYHTGPLSETDSGVLKLWSMDDYVRTMPYQWQPIYTNENYLGVYAFKFLPQK"
     gene            189836..194317
                     /locus_tag="PKL93_00865"
     CDS             189836..194317
                     /locus_tag="PKL93_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNQ69849.1"
                     /translation="MRKVCVLLLILLLAGLTAIAFEIQQYGYELSSSFVEFYSLDYLP
                     SVYTDDKDSFIDIKIYDLDEEDLTGAITIGDVRVSGDPLYAFRQTVKEKQRIYLPREE
                     LMGLRLIVVSSQSGTAKLMTSFRMVGAQLLAHEDGAMLRLWKKDDGSFLDHFSLYRLS
                     DGELLGQSEEGVFVFDYLPKTEEAILVQSSEGSILWRPGEYDYYFSSRHISLTFTDRP
                     AYKAGEVVNFRSFIREVTPSGYKIPEIDFVEIEIVDPLNRGVYAQTLEPDDLGSVVGS
                     FRTYSEITRGSYRIIVRWGENEDYYYFQIADYKKPTFFTLAEPTTSAFRKGEPVTIDV
                     SSEYYFGDPVSLGKVDYTIYKGTQYIDNGSARLDGSGKTVIGYTGELESGAYYAIVTV
                     SDDTGMQSRSVVDFKIVQGSFDFLVDYRFTENEALVRIETRLNDDTPVSKEFEIKAWY
                     EESVILMDKGEHVEKNLKINVFKGELSTDSGGKAEVSIDLRQVPPDTVVYFEINGSPS
                     GEPEIVNTYSVYTGGYYDYYGSILIDSVKSAEPGSSAEVVFYTPSPMDLWIISDFSGE
                     FSQFPFSSTEGKNTVYIEVPEGYAYDNFMLFIVGYRDYQIIQSSLIEVQTSSRELSIE
                     TLTQERYSPGDTVNMKIQVTDSEGKPAEVGLTVSVVSQAMLSIFEGDYDQWKGSLGSP
                     FNRGFNTISINDLYYSAYPSIAKLGDLLESQPPAAESKVTGGAPLGMGDLGGDEKSLT
                     SDVRARKLFSDSAFWSTGTFTDENGQAELSFVVPEDLDTWTVRTLSSSANGDFSYSKS
                     SFETWKPMTVSSFLPEFLIAGDTVNLVFSVKNNLDSTLPVITGFYLGEEVIEEKGSSI
                     KAFESRSFSYEVNVQDLLSSEKDQKLKVKFVVKGSHGSDGVEYEIPIKPRFSYSRFGS
                     LDFLEGNKTLEFSENSAGTITISSTIDPILLEAVRYLVDYPYGCVEQTMSRLLPALAA
                     SQLLEEADTAFINKVSRVVSEGLERLYGYQHYDGGWGWWKDDNSTPFMTAYVMLGLYL
                     ATENGYVINKDVVSMGYSEMKTLYKEDPDPFLQYVIVLFSRKLRDPVGSVVDFKGDVP
                     SIILTSLAYEALNMDDKAEDLIEEAMEYVDLDADDVISGTSFDYFFDDIVTLSLLLKA
                     SVDLEMPSEDIFEISKRLLRMGNGSYWYRTSSTALAILSLSSVSSSLSENAQVEVSLI
                     GGEVLYSGGLSESITIPFCEENVIVESSEMVAVSVNGEKAVAAEEIEPENSGLIVKRV
                     LRRKLAVPMDDTHVLTTPQIDSPYVVSSIKTLSPDEVGSLEINVSKTVENMQISIKDG
                     ELLLGEYGLGLKVYEGDVLGISNGEIIIRAFEESYYDEPTVEIYSVRLGTPGSLTAGE
                     TIVSEVHIKIPEDVPYVVVEDMLPATGISVEENLEADVLGYTKFYYFDYYWWGYTFKD
                     ARYDRIAFFFREGGEFVTKNSWRILTSGEFIIPPVQAWAMYDSNYRANTESIVLVIE"
     gene            194314..194871
                     /locus_tag="PKL93_00870"
     CDS             194314..194871
                     /locus_tag="PKL93_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69850.1"
                     /translation="MKSLALVAFLFFGMTVFCFTSTFVSWEMPELEEYKGLLERLVGK
                     PVTGTYRLIIASSTGEFMELSGLGYWFIAAAEDSTIFVQPLSLVPNLTQTVAHEMCHL
                     FLRQYRLPYWLEEGIVCYVTGEWIGRKELILDRVESLDYSEMDFMTYRSYSFSCWVEV
                     SKILRERTFGELVVALGEGVDEQVE"
     gene            194855..196651
                     /locus_tag="PKL93_00875"
     CDS             194855..196651
                     /locus_tag="PKL93_00875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNQ69851.1"
                     /translation="MSKLNERKIIEWDLTRLYASLDDETIFEDIKTVLREKDELVRDY
                     RGRISGENISARELRGLLEKSESLLNRAGKLSMFAYLNHSVLTADSATQKLVRKMDDL
                     EAALESGLMFIKLELARVSDDFFSELNECDELKNYRHYFDLVRALRDHQLSEPEEDII
                     VRKDITGKMAMLNLFDEFTSTFEYRIFIDGEEKVLTESEVENLRSHGDEGIRKKAFES
                     LFRKTEENSVILTNIYNSLAKDWDLDASKRHFSSPISMRNIENEVSDESVQNLIDATT
                     QGYSIVQDYYRLKARVMNKEILLHSDIYAPLADSRDIFSWQEAKNMILEVMGNFDSQS
                     GRIISGFFDDEYIHGSIIPGKRSGAFCYYASPEVHPYVLVNFGGKTSDVLTLAHELGH
                     GLHGVLSSKQTHLNYETPLTMAETASIFSEMLMTDFLLDNISGKAEKLSLISKRMEEI
                     LASTARQNMFTRFEMAAHSIISREFLSFKELSDLYFEELKLMFADTIVFFPESKFEWA
                     RIPHIFHTPFYCYAYNFAQLLVISLYRMYLEDGDVFKRRYIRLLESGSSDSPEILLKS
                     VGIDISEPYFWENGLHFIQENFLERLRDIIDS"
     gene            196739..198103
                     /locus_tag="PKL93_00880"
     CDS             196739..198103
                     /locus_tag="PKL93_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ69852.1"
                     /translation="MARDLTQGNILKNLLVMSVPTMIGFSAQMVYDIVDIFWIGRISG
                     EAIAGVTIFTTLFWIVDILNSIIGQSSVSLISQSFGKKDLEGTNRAIEQTITFKFIVA
                     LISALLVAAFLKPSLGFFSNDPTVITAALDYGYIRLFFLPMMFSSYSVNTALRCIGDA
                     KSPMYIMMMASALNVVLDPILMFSHIPGTSIPGFGLGVFGAAVATVIAQTASFLLGFF
                     ILFSGKEGVKPKLSRLFRLDRHIDRKLLTIGLPTGLEGFFRNLAAVVVLKFVALYGTT
                     AVAAVGVTGRLFGLAFMPLVGLNMGGSAMVGQNLGADDVGRARATAKTAGLIGFFIMF
                     FFALIAFFGGEIVIGIFNDDPEIISYGASFLKYGSLGISVLAYGFGLSSVFGGSGYNF
                     PFVAGSVVSRWLIQVPMLIVFVAFMKASIVWVWLSYVFSDIAEATVMIFYYLRGKWET
                     RRVS"
     gene            198235..199692
                     /gene="dnaX"
                     /locus_tag="PKL93_00885"
     CDS             198235..199692
                     /gene="dnaX"
                     /locus_tag="PKL93_00885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNQ69853.1"
                     /translation="MSEVLYRKYRPRNFNELVGQDQVKEILGKAIEKNKLSHAYIFSG
                     SRGTGKTTSARILAKMLNCLSEGSEKPCGVCDSCKAIDSSSHMDVVELDAASYRGIDE
                     IRKIRDAVSYRPVMGKFKVYIIDEFHMLTREAFNALLKTLEEPPDKVVFILATTNLEK
                     VPETILSRCQIFYFKPLREEDIVTYLERIARAEELQYDEKALRYISKAAHGGMRDAVN
                     LMERVISYSGNVKEEAARTALGILPEEVIEEFLQAFSSGNPSSVLEISEKIQSGGFTH
                     EVFLEQAIDAVKERLVERPSDDCFRLLSALWETNKELKYAEDKQTTFEVMVLLKGGFG
                     KQNGEDTQKSEVSPIAVDENSEKPEKLEVSVTEESEEISLFIEHLRMKDYILWWTLIS
                     LAEIRKGNRIEDLIIEPDSEFSSELLKERMESINKIAEDIIGKRFVLSDKAAGQEALE
                     SLDRDSREYFESVITGLGLKSDIETGKIKIELEEE"
     gene            199695..200060
                     /locus_tag="PKL93_00890"
     CDS             199695..200060
                     /locus_tag="PKL93_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNQ69854.1"
                     /translation="MAKKFRGLGGRNYGGSRKGSNMGDLLKQAQKAQEEMENLEDSFK
                     SIEVTASAGGGAINVVATCDYRIKSIEVEPEIREEDFEIVQDLIIAGINEALAEVTKR
                     REEETAKITGEFNLPDNIL"
     gene            200073..201068
                     /gene="gap"
                     /locus_tag="PKL93_00895"
     CDS             200073..201068
                     /gene="gap"
                     /locus_tag="PKL93_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNQ69855.1"
                     /translation="MHKVGINGFGRIGRLVFREMVKRGEFDVVAINDLTDAATLAHLL
                     KYDSVHGRFKGSVEAKEGAILVNGKEVKVFAEKNPANLPWKDLGVELVIEATGVFRSR
                     EKTMPHIDAGAKKVLITAPAKGEVDATIVLGVNDEVLKPEMKIVSNASCTTNSIAPII
                     KILNDNFGIEKGYLTTVHAYTNDQRILDLPHSDLRRARTAAANTIPTSTGAAKAVGVV
                     IPELKGKLDGIAMRVPVTDGSITDLTVVLKKDTTAEEVNELVKKAAENELKGIVEFTE
                     EELVSSDIVGTTVSAIFDSKLTAVMGNLVKVCSWYDNEYGYSCRVVDLAKKMMEM"
     gene            201130..203097
                     /gene="tpiA"
                     /locus_tag="PKL93_00900"
     CDS             201130..203097
                     /gene="tpiA"
                     /locus_tag="PKL93_00900"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNQ69856.1"
                     /translation="MSKTLTLKDIDLAGKRVLVRVDFNVPLDKETGEVSDDTRIVAAL
                     PTINEIIERGGKAILVSHLGRPKGKRDPKYSLEKVAERLQSLIGKPVTFVPDCIGEEV
                     EKAVSKMNNGDILLLENVRFYSEEEKNDPDFSRRLSSIADIHVNDAFGTAHRSHASNV
                     GVARYLTSVPGFLMQKEIEMLGMAIENPEHPYVVILGGAKVSDKIGVITNLLEKADRI
                     LIGGAMMFTFLKAQGKSVGDSLVEEDKIDLAREILQKARQKNVEFVLPVDTVIAKEIA
                     AGSESKVVDLEEGVPAGWKGLDIGPSTIELFNKKLSDAKTVVWNGPMGVFEIDDFAKG
                     TESIARTLASLNDAVTIIGGGDSAAAINKFGLAEKVSHVSTGGGASLEMLEGKEMPGI
                     KSLSIEGGKKKRRLMVAGNWKMNKSPNEARMFAGFLASSIGNENAVDVVVFPTSLSVA
                     GVADILKDTSIKFGVQNIYPADSGAFTGEISASMLSDLAVEYVLVGHSERRHIFGETC
                     ELTGEKIKSVLKHGLTPIFCVGETLEEREAGRMKEVLETQIRKGFSGLGKSDLERIII
                     AYEPVWAIGTGVVASPEQADEAMKFIRDLIASLYGVDLSESVRILYGGSIKPENFESL
                     ISMENIDGGLVGGASLQESFVQLVAIAESHA"
     gene            203189..203425
                     /locus_tag="PKL93_00905"
     CDS             203189..203425
                     /locus_tag="PKL93_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69857.1"
                     /translation="MERYQELELVVDKMVEEFTRLRKENEQLWNQLETSQKKVEESEL
                     RIRELELRLESESRTISSLIKRVRGSLVEDSKKV"
     gene            203437..203718
                     /locus_tag="PKL93_00910"
     CDS             203437..203718
                     /locus_tag="PKL93_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNQ69858.1"
                     /translation="MKRSVSLDLGGRKYTFLTSDPQELVDQVFSKIGELYDSLKNNEE
                     EIGYEKILVGISVNLAHDLVRSQNELLRLKAKYEEVLSEYFQGRDEVEK"
     gene            203699..204529
                     /gene="murI"
                     /locus_tag="PKL93_00915"
     CDS             203699..204529
                     /gene="murI"
                     /locus_tag="PKL93_00915"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNQ69859.1"
                     /translation="MTRLRSRLRIGVFDSGIGGLTVLKRLVETLPEGVSFHYFADTAR
                     VPYGSKPVETLRRYVREIFDLFAQLNVDAIVTACNTSDSILTQSDKAELGVPYFSIID
                     PTVRRIGESAPDNSTVAIIATENTVKKSLYLRKLFRYESFSKIVQKACPLFVPLIEEG
                     IWEGEIVDSIVKYYLNDFAEIEPDFMILGCTHYPFIKNSIERFLPSKTKVVDPSEYIV
                     RDVADWATILSPEPSTVDYYVSGNTRIFQDILKRYLGRFENVKGVDLNVTGINVRGRV
                     "
     gene            204531..205493
                     /locus_tag="PKL93_00920"
     CDS             204531..205493
                     /locus_tag="PKL93_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNQ69860.1"
                     /translation="MSKIVLVGGGTGLSTFARVIKDFVTSLTLIVAVTDDGGSSGILR
                     EALGMPPPGDVRNNIIALADDEELLTKVFSHRFSAPAMNGHSLGNIIIAGLMEMCGGF
                     SEAVLTASRMLKIKGIVLPVAEDLVSLVGELDDGTIVKGESRVSAAGKRLKKVSLDRA
                     CEALPEVSDSLVSADTIIIGPGSLFTSVVPNFLVSGVKEALKRSQARKIYVCNIMTQP
                     GETDDFSLAKHVQIAESYCGVEFDLIFWTEIGGVASSVLSRYEEKGSRPVENDMISDG
                     RVKVITGATTEFIDDGRRRPVLRHSRAGIISILHELGLLGEEGI"
     gene            205490..206416
                     /gene="whiA"
                     /locus_tag="PKL93_00925"
     CDS             205490..206416
                     /gene="whiA"
                     /locus_tag="PKL93_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HNQ69861.1"
                     /translation="MSYADKLKEELCHLPLDDQAECRAEYLGFVKSRGTLRLRGNTSF
                     LVIPLTSITSLKRLFQVSKRLSIPIFETQVIEEVRLDRKRGGELSYLYGEVEEFLRRN
                     GISVRDDSIPRPVREDPVYFGAFLRGLYLAGGSVVDPSKEYHLEITLDTTEAFVNSVK
                     QYVSENFNIKVGVVKVRDKFKAYVKSSMDIIELLSLMGGKKTVTRLSSAIEVRKIRSD
                     VSRTLNFLTANANKSGQAMAKHVKAIRIVDRKLGIENLEEDLQQVAILRLENEDLNLR
                     ELGEIMNPPMSKSAVYNRLKKLMALAEELGDK"
     gene            206417..206878
                     /gene="nrdR"
                     /locus_tag="PKL93_00930"
     CDS             206417..206878
                     /gene="nrdR"
                     /locus_tag="PKL93_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNQ69862.1"
                     /translation="MKCPFCGGDSTRVLDSRPTDENTSIRRRRECEKCGARFTTYERY
                     ERLPFLVVKKDGRREKFSREKLLNGLLRACEKRPISVDTVNEIVDSVENEVVKSGKHE
                     VVSSEIGEMVMTKLKSLDRVAYVRFASVYKEFRDLDHFMDIIEELRDDRNR"
     gene            206896..207369
                     /gene="greA"
                     /locus_tag="PKL93_00935"
     CDS             206896..207369
                     /gene="greA"
                     /locus_tag="PKL93_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNQ69863.1"
                     /translation="MKKKVIYLTQEGFNGMKEELESLRKKLMYDIAERIKEARELGDL
                     SENSEYDEAKNEQGRIDSRIKQLEEILNNAEIIEDDGDLSTVKLGYVVELLNIDTNEK
                     SQFRIVNAQEANIFEGKISSDSPIGRGVLTHKVNEVVRVKTPAGWAKYKILTIGK"
     gene            207382..208887
                     /gene="lysS"
                     /locus_tag="PKL93_00940"
     CDS             207382..208887
                     /gene="lysS"
                     /locus_tag="PKL93_00940"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNQ69864.1"
                     /translation="MSEETRRQRIEEINQMRQEGIEPYPYSFDKTHWAAQIKSEFAYL
                     QNSETKEDTVLSTAGRVVAVRNHGKSSFFVLRDNSDRIQAYIRLDGVGAESFEVFKKY
                     VNIGDFVGVKGFPFKTHTGEISVFVKEFEILSKSIRMMPEKWHGVKDKEIIYRQRYVE
                     MLSSDDALKRFKIRSELFRLVREFLQSKNFVEVDTPMLHYLTGGASARPFKTHINVFE
                     SDMYLRIAEELFLKRYLVGGFERIFEIGKNFRNEGVSYKHHPEFTMMELYWAYADYND
                     IMNITEEMINFVVREITGSEIISYQGKEIDFSRPWRRVKMADFIKENLGVDILEDSDD
                     RMMAVLKKHNTEPEIKERGHLIEKLWDLVEHNLVNPTFVTEHPVIISPLSKVHREDSR
                     VTERFELIISSMEMANAFSELNDPIEQYNRFLHQAGLRDIGDEEAQMMDHDFIRALEY
                     GMPPTGGLGVGWDRIIMLVTDSPSIRDIIPFPLVRPISFEEEEALEISDEQ"
     gene            208898..209359
                     /locus_tag="PKL93_00945"
     CDS             208898..209359
                     /locus_tag="PKL93_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNQ69865.1"
                     /translation="MTLYDRIQQDMKEAMKARDSLKTNTLRSVISSVKMYLASSEEAR
                     SGELTDEIVIGLVRKEAKKREESIEAYRKAERDDLVQSESEELEILKSYLPEEMSPDE
                     IRAVALKTIEDLKANNPSDLGKVMRELMVRLKGRVDGKLVNKIVRELLESR"
     gene            209356..210726
                     /locus_tag="PKL93_00950"
     CDS             209356..210726
                     /locus_tag="PKL93_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HNQ69866.1"
                     /translation="MKVEFLTAEIGSTTTVLSAFSCDASGSMRFVGQGESYTTVNEND
                     VTIGIDNALRNLKRSLGCDNLEWSVFLASSSAAGGLRMTVHGLVYDMTVKAAREAALG
                     AGGVIKLVTAGKLSERDLKKIEAANPKLILLAGGVDYGEKETVVHNAEILRDLDQNIP
                     IIFAGNVAAADDVLEIISTNGRDVHIVDNVYPRIDELNVEPTRQVIREVFSKNIVNGP
                     GMEKVYSVIDKPIIPTPAAVMLATELFSQVNDDVIVVDIGGATTDVDSVTDGDPEIAS
                     ILLAPEPRSKRTVEGDLGIYVNASHVIEQIGRDELGKEFPDLENILRNLTPYPKNDRD
                     EAFSLKLAAYCFETSIRRHAGTIKQLYGPTGRTEVAYGKDLTAVKSVIGTGGILSRSK
                     FSSKIMKIVRTLSAKHEKELLPKSSVRLFRDRNYIFGAVGLISTVDLPSARDLVENSL
                     EELNTT"
     gene            210815..210890
                     /locus_tag="PKL93_00955"
     tRNA            210815..210890
                     /locus_tag="PKL93_00955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:210848..210850,aa:Gly,seq:ccc)
     gene            complement(210892..211392)
                     /locus_tag="PKL93_00960"
     CDS             complement(210892..211392)
                     /locus_tag="PKL93_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNQ69867.1"
                     /translation="MKVIAVFASTLNGKISLSEDDKTEWTSREDKRYFKSLTSSVGIV
                     IMGRKTHEAIGRALPDRLNIVLTRNPDYYRPSENLIFTSSTPKELLEDLEKKGHDEVC
                     LIGGAETFDHFADLGLVNELHITFEPIIRTGIAGLGETMERDLDLSLKEFRKLGEAIL
                     TVYEVK"
     gene            211475..213445
                     /locus_tag="PKL93_00965"
     CDS             211475..213445
                     /locus_tag="PKL93_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69868.1"
                     /translation="MIKICYSSLDGRVKEIKRIAWRVLIFAMLLMLISCSNSTNYAVS
                     IKQIDCEGSTCNLRLEISEDGLSLPEFLITLDRPPVDGSDGIRIKGTEISLDLKEAGS
                     HTIFVTLVDGKRFLSTPVSKEVIINLNPPDAPDLTWSLSQGELKLSLRSDSSDSVTDY
                     RILVNNVLHSSTQGTFSFPVDRDAHYVIQAFSVAGNLRSDPSILDLKLSEDFAPEVEL
                     TSVLPFTNGAVDITVVDDWDRTNDLSIKAFLEESNILLLFDGNRLIPEIPLPEGINTL
                     VVRVADSSGNVTELKRELKTVRKTSVEIPELYIEEGTIRNARWTTPKGMVELQRYIDG
                     EWITLSTHSSEEESSIIAKDSISEKGDLYRIVVDSDNEKLLPSVPVFAKESLFRRFST
                     EFISSFLGMDALLVGGNSYRFYGNIVVRENTVMRVESGTSLTIPRGNTLLVSGVLDLE
                     GWKDRIKVNPGGSSGTIEVTNNGVLIARSVDFNNSTVSIENAAVAVFEDCTLSGDLKI
                     SGARTVQIYNSQFSGDMEIENVRELLVHQSDFSGSSLVLSGLGRVLLSRSSLRFHSLE
                     IVDSRLEAYESLFEAEGLFVRKTSHLLQTRGALIETEISVSGASSIHIEEPNLTGDVK
                     IELRDLSRLSLQKDFEGSIEIVSDEKSAIKVF"
     gene            213447..214124
                     /locus_tag="PKL93_00970"
     CDS             213447..214124
                     /locus_tag="PKL93_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ69869.1"
                     /translation="MGKKFRDFDPYVLEAFEFDFSSRHARINHGTVLLSWFVEPPKGS
                     RRALELGAGSGVISIYLAKNFGLEVTGVEVDRELHAVATKNASLNGVENLTRFVNCDI
                     DDYAANNRGEVFDMVVSNPPHFLHSGVESPDGKRNRARRIDPELAMVFASATSKLLKN
                     RGAFFFLLHPRDLTKWVRLLEDFKLGIHKMRFAHGSMEGQAQLVLVSGRKSSSSEIVV
                     EPPIRMR"
     gene            214129..214758
                     /gene="tmk"
                     /locus_tag="PKL93_00975"
     CDS             214129..214758
                     /gene="tmk"
                     /locus_tag="PKL93_00975"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNQ69870.1"
                     /translation="MFISVEGIDGCGKTTQIKLLAEYLSQLDVEFIIVREPGGTKAGE
                     EIRDILLHKEYHLFSESELLLFMAARAQIVREVIRPELEKGKTVIADRFMDSSVAYQG
                     VGRRLGREIVQAMNAFAVGGTVPDITFYIDVPTDVAISRMRKEKKNDKIEMENFDFFE
                     RVREGYEKLMVENRERFVRIDGKQSEEKVQTDIRKSLRVLFHKKNQNGR"
     gene            214764..217253
                     /locus_tag="PKL93_00980"
     CDS             214764..217253
                     /locus_tag="PKL93_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNQ69871.1"
                     /translation="MYFDKFSEGAAQVFVTAQDEARSLGHPYVGTEHLLLAIIKVENE
                     LTTRILRNYSITYERISREVTSMVGTNVHQSVVGAPQMTPRARRVIELANDESKMLGQ
                     DKIDVEHIMLGIVREGEGIAAHILKQMGVNLSQLRREIIENITGDWEEGGEQPENVFV
                     QPSSTMGPAIKQLEGFGIDLTAMAKAGKLDPIVGREIERQRLMEVLSRRKKNNPVLIG
                     EPGVGKTAIVEGLAQMIVDEEVPETLKNKVIFALDVASLIAGTKYRGEFEKRLKKLIH
                     VVKTNGNIILFIDEVHTIVGAGSAEGAVDAANILKPALASGEIRCIGSTTPDEYRKYI
                     EKDAALERRFQKIYVTEPTPEESVEILKGIRHKYELHHKVHYTEKGLEAAVTLSHRYV
                     SDRFLPDKAIDIIDEAGSRARLKKLTIPDELKKMQGDIESLKIKRETAAADQEYEKAA
                     QLKEKERLIQQKYRDGYNDWRNRVDSEIVTLDDDEVAEIVSNWTGIPLRKLEETDSEK
                     LLTLENALHERIVSQDEAISAVAKSIRRARSGLKDPRRPIGTFLFLGPTGVGKTELAK
                     ALAEYLFGDDKALVRFDMSEYMERFSVSRLIGAPPGYVGYDEGGTLTERIRKRPFSVI
                     LFDEIEKAHPDIFNILLQIMDDGRLTDSQGRQVDFRNTIVIMTSNLGGEFINKTKSSI
                     GFVESSHEEKEYEAMKTSVLEEVKRTFRPEFLNRLDEVVVFHQLTKDQIKTIIDILMN
                     DMRKRLNEKHLKLILSEAAQEFLVEEGFNPVYGARPLKRAIQKYVEDPLSEELLRGRF
                     QDGDLITAEVDNRVLIFKKKKDEKTEVKAIT"
     gene            217349..218674
                     /gene="radA"
                     /locus_tag="PKL93_00985"
     CDS             217349..218674
                     /gene="radA"
                     /locus_tag="PKL93_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNQ69872.1"
                     /translation="MKKKRSYVCDVCGYESPAWFGRCPSCHEWNTAISFVSEGDKDEN
                     ISVADIEVRPLSQVDTTESSRIDTKIEELNRSLGGGVVPGSAVLISGEPGIGKSTLML
                     QLSALIAGEKPVLYISAEESARQLRLRAERLKIQRKEQIEVVSATEIDFLGSLAIEKY
                     GMIVVDSLQTIKMSSVPSLPGSVVQVRECANMLVSLAKSSDIPVLIVSHITKGGQIAG
                     PKLVEHVVDTVLYFESSKTGFRFLRTIKNRFGPSDEIAVFEMTSEGLKEVPDISSLFV
                     EDYISAPGNVVSAIYEGSRAMLIEVQALVSKPVYGTPRRLASGIPLERLLLVTAVLTK
                     RAGIPLDSLDLYMNVAGGLQIEDTGVDLAIAAALISSYTDTSIPEGVAFFGEIGLDGT
                     VRSVSSMEKRIERAKKSSFMRIVAPEGRNGIKDVKQLLKFIGGMKSGKE"
     gene            218661..219740
                     /gene="disA"
                     /locus_tag="PKL93_00990"
     CDS             218661..219740
                     /gene="disA"
                     /locus_tag="PKL93_00990"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HNQ69873.1"
                     /translation="MERSELIEKIKKIAPGTPLRHALDDIQDANVGGLLFFVDDYKKY
                     SHLMQIGFLLNCQFNPNKLYELAKMDGAIVVDDDLTTIVGANVQLIPDHTIPTNQTGM
                     RHRAAERMAKETGRMLVAISKRRNTMTIFLGDFAYTLNNVETLTSKITQTIRTAEKYK
                     EAFIEALQGIEFMERAGSLTLYDVVKTIQKGLMVALICEEAEFTIAELGNHGRFPSMQ
                     LTEILVNVNEEIESLILDFHEELLDQDSLEEVFDKMRSLGEKEISSFGVILRNLGYDI
                     NTASQAVEYFIRSRGIRFARHIPRIPLQVAINLGKEFGTFSNLLESSEDELKDVDGIG
                     EKRASAIRHAIDVYKKSGEITVPGF"
     gene            complement(219721..221007)
                     /locus_tag="PKL93_00995"
     CDS             complement(219721..221007)
                     /locus_tag="PKL93_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNQ69874.1"
                     /translation="MIVTNVLAFTNDLKNPLVDDALVRFEDGEITFVGPRREYSIQED
                     EEIVDGEKGILLPSFFNAHYSIYSSISPYPVRVLKGERTEIDFFGGLLNSGRKEVNSE
                     LVRLSAELAVMKAIRSGSVAMSGPVVDHPDVDPELYREIAAKYGVYLAVGPVLTEDTL
                     DEIGGRWENVTRDASFCPMLYVSNLATFEEEAIQKLRGLYEKGMNLNLLIFDMKRENE
                     VCLFRWGESLVERLMKNGILIPECGVVYGGNLSETDMDIISSRRIFVTKSLRSEMYAG
                     TFQPNISDLLGRGMQVCLGTGFLGADILGEAKEIILTERCHRGFDNSIIDYEIRKTLF
                     DNNYKLFQRFFGKHSGVLKEGYSADLVLAKSSLDLEKFDMNLPITLQLVLKSSFDTSF
                     EKVWNSGELIFDKGVPQRISKKELREISKQLRSLEL"
     gene            complement(221004..221744)
                     /locus_tag="PKL93_01000"
     CDS             complement(221004..221744)
                     /locus_tag="PKL93_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNQ69875.1"
                     /translation="MRFLDFELEEKDLVSVANELIQRTQSGVRSHVLTINALIAYEYM
                     HNEEYCRALHKVNYVVPDGSGVLKAIKLISKRNLERVPGIDLMLKLCELSAEKGLSIF
                     LLGSKETVVTKTTEKLKQRFPLIDIVGHHSGFFTQEENDTIVSEINASSADILFVGMG
                     VPRQELWIAENAEKLQAKILMGVGGSFDVVSGNLKRAPLWMQRAGLEWLYRVVREPGK
                     RLKIIPKLFSFETYVIRSYLESRRGGSR"
     gene            complement(221819..221908)
                     /locus_tag="PKL93_01005"
     tRNA            complement(221819..221908)
                     /locus_tag="PKL93_01005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221872..221874),aa:Leu,seq:caa)
     gene            complement(222011..222712)
                     /locus_tag="PKL93_01010"
     CDS             complement(222011..222712)
                     /locus_tag="PKL93_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HNQ69876.1"
                     /translation="MRRLLIAVYLVFALAVAFSYTEDLPEQIFLSNGNMISVLGKNIV
                     EDSEVCEINIQIPSISGLKDKPFEEYLNATIESEITDYRTQIEEMAQAAYEESKTSEW
                     PFRKFSIYVLYEAYLSDGILSIDMIFSEYTGGAHPGASRRTYNISVDRCRLVALHSVL
                     DSAAAEDRLNELITGEIGKREDLWPEYFKGVTTDQGFYLKAGRLCVYFQPYEIGPWAA
                     GMPEFCFSLGELLKQ"
     gene            222998..224020
                     /gene="glpX"
                     /locus_tag="PKL93_01015"
     CDS             222998..224020
                     /gene="glpX"
                     /locus_tag="PKL93_01015"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNQ69877.1"
                     /translation="MIDLIPEITLDMVRVTEAAALFSSVFLGRGDKEAVDQRAVDAMR
                     GILDLLDMKGTVVIGEGEKDQAPMLAAGEHVGKWNEDDPEVLIAVDPVDGTRLVANGR
                     SNALSVIAATEDGKIAPLPTYYVDKLAVGKELAGNLDIEATPRENLRIAAAILGVNVS
                     ELTVTILDRERNHPLVEAVRTVGARIRLINDGDIAAAIATALPDGGTEIYMGIGGSPE
                     AVLAAAALQCMGGEIQVKCWPRDKDEIEKVEKSSFELHAVYGTKDLIDGEEVVFSATG
                     ITDGSFLKGVRFTHSQAITDSIAMRSSTRTVRRIRAYHEIAYKTITTKSGGEVSIFNS
                     LLGKRA"
     gene            224483..225076
                     /locus_tag="PKL93_01020"
     CDS             224483..225076
                     /locus_tag="PKL93_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNQ69878.1"
                     /translation="MICILQNDSDFYKEISPVEKALDNTGYEYSTWRTFLDGFPEDEL
                     SCDGLIITGGFSMSNYFEGRALSSGGNFVRSFRGPILGICLGMQILARIGGESLVVSK
                     ELGVTKIRLDALHELFSGIESEVSAYQRHNYGLPYVPYGYRQIAWGDETFIQGISSTD
                     GLRFGIQFHPEEIELGSDEQLQRIFANFCRIVSFHKR"
     gene            225091..225921
                     /gene="proC"
                     /locus_tag="PKL93_01025"
     CDS             225091..225921
                     /gene="proC"
                     /locus_tag="PKL93_01025"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNQ69879.1"
                     /translation="MRVGIIGVGNIGGIFADRLLGELEGLEKLYLLDRNQERLSNYSG
                     ETERVVIAKTPGEVLENCTHVIISVKPQDCMELFAEVINGKNSSPIIISTITGIEIAL
                     ISRKTGLEKIARIMPNVPSAIGRGVTGFVCSSSLTGREKKEVERILRTMGEIVEVREK
                     DLSAVTALSGSGPAFVFVIVESLIDVGLKMGLSYDVARELILGTLGGSVELLKVKGNH
                     PGEFRHLVTSPGGTTIEGIYSLEREGLRGTIMKALFDTYLRAEEINSELEVTHNGSSE
                     "
     gene            225905..227146
                     /locus_tag="PKL93_01030"
     CDS             225905..227146
                     /locus_tag="PKL93_01030"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNQ69880.1"
                     /translation="MDLLNKGERVKTASRKLRLLSTNEKNEAIMLISRELETQKGEIL
                     RANSIDVENSRKKGMKESLIDRLALSEERIAMMVKSCENVASLPDPVGEIESAWVQKD
                     GLLISKERVPIGAIGMIYESRPNVTVDASILAIKSGNSVLLKGGSDAINSNKAIVSAI
                     KTALALAGLPDSSVELVEDTSHEAVQEMLKMNQYLSLIIPRGGAGLIKFVVNNSRIPV
                     IETGTGNCHIFVDYNADLNKAVEIVDNAKTQRPGTCNAVETLLVHRDIAAEFIPRVSE
                     VLSKKGVELRGCDESTKYWKMISATDEDFATEFLDLVLAIKVVKDVDEAIMHIEKYSS
                     GHSEAILTNDYANSRKFLKDVDSAAVYVNASTRFTDGGEFGFGSEIGISTQKLHARGP
                     FGLKELTSYKYTILGNNQVRK"
     gene            227146..228207
                     /gene="proB"
                     /locus_tag="PKL93_01035"
     CDS             227146..228207
                     /gene="proB"
                     /locus_tag="PKL93_01035"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNQ69881.1"
                     /translation="MSRVVLKAGSNLLVKSNGEIDKKYVTELAREISLLKSSGSQVVL
                     VSSGARAAGFGYLEGKKSNGDLYMKQALCAAGQVQLMKLYETVFDLFGERIAQILVNR
                     DDFGDRKRFLNLRNTLIGLLELGLVPIVNENDTTSTEEIMFGDNDILASMFAIGWQAN
                     LLILMSTIDGILDEEGKVIREFDPSKRLKNIENNSWGTGGIDTKIRAARAASASGIRS
                     CICNGRDFRKIEEFVQGKDVGTIFSPIESPGARKTWIGFLSTPRGKITVNRGAEGAIA
                     RGKSLLAVGVEDVSGEFSEGDVVSICSVEGKEVARGISNFSASEVQKIKGVRSDRISS
                     VLGHDCAKVVVHIDNSYRI"
     gene            228441..229781
                     /locus_tag="PKL93_01040"
     CDS             228441..229781
                     /locus_tag="PKL93_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNQ69882.1"
                     /translation="MYSFPDLLYSDVRIEDVSKSDIVVTLGRTDNMKEQRYSAAFIRV
                     FDGERWYFSATTDLDSIQDELDRLASLAKKNRSIEEHGVVRKLQANKGSYLRFDGDLN
                     VFKTSLNEKYDILSSYFPHVEKSELVKFWRGQYIDQRVGKRFFSSKGANLEFDYQRVG
                     FRLLFQMAKDEEQFMERYDLAGNELDSIKGLHEEVRKTVETSEDFIRNARPVVSGSYP
                     VILSPEAAGVFAHESFGHKSEADFMIGDRTMMEEWKMGRRVGSEILSIVDDGNKPGVG
                     NVIFDDEGTKAEETYLVREGLLSGRLHSAETAAALGEELTGNARAVNFEYEPIVRMTT
                     TFISPGELSFDELLSGIKEGVYVDSLNHGSGMSTFTLAPCRAYMVRNGKIAEPVKISV
                     VTGSVFQTLNEIEGLTEEFKLLSFALGGCGKMEQYPLPVGFGGPYVRVRSLSVH"
     gene            229794..231029
                     /locus_tag="PKL93_01045"
     CDS             229794..231029
                     /locus_tag="PKL93_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNQ69883.1"
                     /translation="MTKERYVVHYTENAVKIVQTQVSSVRKKDILRTSIRLYDGKSMG
                     VAGALGECNEEELVAKAKESLKLAIPYPYEASRELVREERHRSDLQGEEELLEATEEI
                     LQKLRRDQPHFSFSHLFKSARVESSMQNDLGLNLKSSREFISLELVIKDKKSSNIIDA
                     MTGYEGRKFHKDSFIELTNMICDAYMNQLPDIEEGVYPVVFRSDDMTYYKKLYESLNG
                     MLFGSGGSLLSGKIGKKVFSDKVTLFQTRNVEDGFFEPFFDKEGTVNDSDRFALIEDG
                     VVISPYTDKKTAATFNLTHTGSAGGEYDGLPELGMPMLKFAESHKTAKQLLDGQKAIF
                     ILIASGGDFTQDGRFATPAQLAFLFDGDRFIGRLPELSLSSHIFDMLGKDFRGVSSED
                     FLGIENAKLTIMDMNVKKL"
     gene            complement(231026..>231237)
                     /locus_tag="PKL93_01050"
     CDS             complement(231026..>231237)
                     /locus_tag="PKL93_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69884.1"
                     /translation="DRLRPAFDWIDYPEGISFIDGPVGPRMDFPIIPDPSIAIECKPY
                     LPGFSVMSSQLFEEYRLHGIRGTEI"
BASE COUNT        64539 a        49714 c        53561 g        63223 t
ORIGIN      
        1 attcctatac taccagtacc acttcctatt gtcaagttac aggttcgttt cctctaactc
       61 atcgcttgag tgaattcatt ggagagtcat gtacggtcag atgtagtcgg agagttcatc
      121 ctctatctct ttcaaagatc tgttttcaag gattgaaatt ctcattgcga taggcggatg
      181 tgtactccac aaagacgcaa aaaaggaact cttgttgttt atccttctct tcaaaggatc
      241 ggagataaac agatgagctg ttgctacatt ggcggtttga agcttcgcag aattggtaac
      301 gatctttctc aaagctctgg aaagtcccaa gggatttctg gtcagctcaa cggcccgcgc
      361 atctgcaaag tactccctcg ttctagatac cgcgaggaga gaaagccttc ctatgaacga
      421 gaataacgct cctaatcctg cgaccgcggc aagaaaggcg attatcgcaa gcgccgaatt
      481 gtcatttttc tttgaggaac gttttgtcct tgacgtagca ctgatcgctc caaatctaag
      541 atgagcaatc aaagcccgaa aggctagcga ttgaactata accactgcgc caagaagcgc
      601 ggaaattaga gtcatgagta aaatatctct atttactatg tgactcaact catggccaat
      661 tacaccggaa gtctcctcac gatcaagact cttcaagaga cctgtagtca cacacactac
      721 cgagttttcc tgcttcctac cagtggcaaa agcgttgatc actgagtcgt cgtctatcac
      781 gtagatttcg ggtggctttg ccattcccgc cgccagggaa agttcgtcaa caatatttct
      841 taactgccgc tcttcgggat cgtctgttct tgcttcacga gccttcaccg atttaagaac
      901 aatatccttt ccagaagaga gggagatcaa agtctgcacg agagcaatgg caagaaaaac
      961 aaaagtgaag acaggcaata tcccaaatat cagatcgatg atcaaaccga aggcaatcat
     1021 catagcaagg aagaccatca caaggacgat ggtcttcctg acatttttcc tgctttcttc
     1081 gtagaaatct acggtgaaag acataactct cagaagttaa tatctacgtt gggtgtttct
     1141 cgttcggctt cagtaacttc aaataacggg aactgctcaa actttcctcc aaagaagcct
     1201 acgatgagat tgttagggaa gatctcgatt tttgtattga actttgtagc agaatcattg
     1261 aagaactgcc ttgcgtaaga gattttgttc tccgtagcag ttagttcttc catcagttgt
     1321 gagacattag cattcgcctt aagatcaggg tagttttcgg ctatggcaag aagtctacca
     1381 agcacaccgc cgagttcctg ctcggccttg atcctatctc caactccttg agcggacacg
     1441 gcctttgctc ttgcttgaat tactttgtcc agggtttcct gctcgaattt catgtagccc
     1501 ttcactgagt tgacaaggtt cggaatcaga tcatgtcttc tcttgagctg aacatcgatc
     1561 tgcgaccaag cattctctac tctcttcttc agggtaacta aattgttgta aaccccgatg
     1621 agccaaagag ccaagacaac gatgattcca attacaatga aaagaatgac cattcaacca
     1681 cctccaacgt atggctatta tacagtattg cctctttcaa ttatctctat gatcgctcca
     1741 atgttattga caacttcgta actatgtgaa tcaggtacca tgtccgctgt tgtttctcca
     1801 gacaaaacga gatatgtgtc tacgccggct ctaagaccgc actcgatgtc cgtgtaaatt
     1861 ctatctccta tcatcactgt ttcctccgga ccgtagccaa agtcatccat caccattttc
     1921 agtatagtcg gatctggctt gcctataatg ttatcgggcc gacgacccgt ggaagcttcg
     1981 aaaagactca agaaacttcc aacatcaggt accggtccat cctccgtggg acagttgacg
     2041 tctgggtgag tcgctatgaa aggaatgcct tgccttatga agagagcagc tttccggatc
     2101 ctctcatagt ttattgtcgt atcgtatgca agaactaata attccgggga attatcgtct
     2161 aagttcactc cacttcttct gaacatttcc cttaccgacg gcgtcgagag caaaaatact
     2221 ctcttatttg gaaaatctcg cttcaagaag cttatggtag cttcgccgga ggtatagact
     2281 tcgaaaagag tcctatcaac tcccattttg gtaagttttc tgatatattc ttcaggtgtg
     2341 cgtgatgaat tatttgttag aaacacgagt ttggaaccga gacgatgaac cgcattggcg
     2401 aattcaagac tgcccttaag aagtccgtcg ccaaggtaga atgttccgtc catatccgaa
     2461 acgaagagcc gtttatttgt cagcatctct tcctccgttt ttcaggttgt tttcataagc
     2521 agagtataat acaaagggtg agatgaaatg aaaaggaaat tcgaaagaaa gtacttcttt
     2581 ataatattca tgatagtaag cggcttcttc atattcattg ccctgggtaa cggattctgg
     2641 agagaatctg ccccttacaa gatagattca gtcgagataa atgcaaagct cgacgaacaa
     2701 ggtgatcttc atgttgaaga gctttctgtt tacatacttt cccgtaataa tcgcacggca
     2761 tccaaaaaac tacacttatc ttacccttcg gttctgaagt cttattcggt tgaaattcct
     2821 aatgatgctg gatttgtgaa agagatctcc tcttctccgg gaggttttga cgccaggatc
     2881 ttccttgaac aggaagtgag tgagtttgtc atgaataccg aatacgttat ttcgggagtc
     2941 gttgaggtgg gttcggatat agctgtgtta agctatagat tctgggaacc gaattccgac
     3001 gctatgacaa gggatatttc tctcgctatg gagcttccag aaacgataat ttcgagactt
     3061 acagaggatg atatttctgt tagaccattt aaggaagcga gttttgaaat tgaaggaaac
     3121 cttgtcaaac tgcaaatgaa gtcggttctt tcaaatgctt ctggcgaaat caagatctca
     3181 tttccgaaga gcattgctaa tacgatgcca aatgtggtca atacaactat aagtaaaagt
     3241 gcattgcgta aggaacatga atccgctatg ttccagcaag cctttctttc tggtttgatt
     3301 ggcttccaaa tagcagttcc tttcttcgtg atcttcttca cattcatact tttcgggatc
     3361 gaaccacagg tgaagtctga aataggctac agactctacg atgtaccggg ttaccttctc
     3421 aatgctgtaa tcaagaatcc ctttcaggaa attgaccata acgggtttct atctacgatt
     3481 atggagatgc ataacagtgg ggagatacag attggtgaga acacaatttc tttcgaaaca
     3541 agtgaaaaag gaattccgac acagcaaaaa tgggctttgc agacgctgaa atctcttaga
     3601 agaaatgagg atggatcgat agagatccct gatgcagaga gcgacgcttt ttcttttgac
     3661 gagttcaaag aacaatacgg tttctggcgg aagcatgcta tgcgtgttgt aaagtcgggg
     3721 cgattctatg agtatctggg ttcaactgta atgtctgtgt tttctatatt gtatctaata
     3781 atatggtcac tgatattgaa attcgtcttt gggaactggc aagtttatct ctccttcccc
     3841 gatgaatcgc tggcgatttc aatagtcctt tacgtcgatt ggtgtctcgg ctggctactt
     3901 ctcgttcttc caaagaatat ctttgctcgg tggacgcctt ctggaaggtt gttctacatg
     3961 gaatggaaaa aaaccgagaa ggagcttctt tcaaaacagt catggactaa tgatgacatt
     4021 gcaaagctag ttgctcttgg tcacctacag gaccttattt ccaacaggaa gagagtatct
     4081 gatcaacagc tcactttgct gcgccagcta caaagactcg agatgatttt taacaaggtg
     4141 agaccttgat tcctacattg aatttgagct gattaacccg aaagtgaaca gggttgacaa
     4201 ctgaaattct aatcctttat aattagttta tgatacccct cgggggtaat ggaggtgaag
     4261 aatcaaatga acgcaaaggt aattgtgtat tccacaccgt cttgtccttg gtgtaagaga
     4321 gcaaaggact atttcagaac caatgggatt cctttcaagg actacgatgt gagcaaagat
     4381 agagctaaag cagaagagat ggtaaagaag agcggacaga tgggtgttcc agtcataact
     4441 attggtagtc aggtaatagt cgggttcgat aagaatagga tagataattt gctgggaatc
     4501 cactaaggtt aaatcatcag accatctgcg ggcttaggcc cgctttgttt ttttaccggg
     4561 gtagtgctat tctctcaata tagggcaaaa agacatatgt gcgatgttgg aggtgtagtg
     4621 ttgaagaggt attttgttgc gcccggtaga gttaatgtca ttggtgaaca caccgactat
     4681 aaccagggat tcgttatgcc tgtcgcgatt gacaaatatg tattactgtc gattgagcgg
     4741 acagacgaga gcgtcattgc tttgagttct gctggaaggg agccagtctc atttcgggag
     4801 gaaagcatag aaaagaccgg cgattggagt gactatctaa aaggagtttt ctgggttttg
     4861 aaggataacc ttggcgttga ctttggaggg atgaagatca aaatcagctc tagcatccct
     4921 gaaggtgccg gcctctcaag ctcagctgct cttgaagttg ctcttatctt agcgttgaat
     4981 tcggtgtatg gacttaagct gggggacaaa gaactttata gttttgctca gcaggccgaa
     5041 aatgagtttg tcggtgtcca gtgtggaatt atggatcagt ttgcagccgt gatgggaaaa
     5101 aaggacacgg caatcttcct agacactctc gagatgcgcc acgaatatgt accgttagag
     5161 cttggtgaat acacatttgt cgtattcgac tccaaggttc atcattccct atctagtggt
     5221 aactacaata ctcgaagggc cgaagcaaat aaggctctcg aaatactcgg gaagaacagt
     5281 tatcgagagg tctccatgat tgatctcttc cctaacagag gtaaacttgg tgatctatac
     5341 tacagaagag cgctacatgt tgtttctgag aatatgaggg ttctagaggc catgaagatg
     5401 atgtcgaact cgaatttcga gaatctcggc cgaatattga ttcagtccca cgaatcactg
     5461 gctcttgatt atgaagtaac ttgcgaagag actgatttta tcgttgacac tctacgagaa
     5521 attggaggtg tctccggtgc aagaatgata ggagcaggat tcggcggttc ggtactcgct
     5581 ttatgtgaaa agattgagat gaaaaaaata gttgaagtca tgaaaatcag atacaaagag
     5641 agatttggaa ttgatctcga ctcttatgaa gtaagaccgt cagatggagc gagagaagtg
     5701 gatgccacct tcccgcttta atatgtgttt ggacagtcga gcagagagta ctattccatc
     5761 cgtctctttg tagttctttc taaggaggag ttgcatgaaa gtcaatttgg gtattgtggg
     5821 tgccggaata gcatctaggg agctccatct gcccgcgttg agaagactga ccgagttatt
     5881 caagattacg gccgtgaaca gcaggactag aagaaaggct gaggaatttg ccaaaatagc
     5941 tggtggcgat gttgaggtct ttgattcgta tgaagaaatg ctgaattctg atgttgtgga
     6001 cgctgttgtg cttgcagttc caattgcctt gaatccacaa atgattgagg cggcaagaaa
     6061 ggcaaacaag ccggttatct gtgagaagcc agtagcggcc actactaagg aagccatccg
     6121 attgttgcat cttccaggta atactcccat ttatatagcg gagaattaca ggcatatcga
     6181 ggtttacaga aaggccgaaa tacttatcaa ggagggaaga cttggcaagc ctctggcatt
     6241 cagctggttg aagtgggtag atttcgggca ggacaacaaa tacgttcaga ctaagtggag
     6301 gcagacgcct cagcacatcg gtggattcat ctctgatgga ggggtacatg atgtggccgc
     6361 gcttcgcaag attcttggta acgttgagga agtaagcggc ttttcggaga agaatttcga
     6421 ctatcttggt gctgaaaact ccgcggtctt caatatgact ctgggttcca atgtgattgg
     6481 taactattcc gtcgtctatg gcgcccccgc ttctctaaat agatttgaaa tagtctgcac
     6541 agaaggaata gtccaagttg acaaagacga agctactctt gagatatatt ccagcgacag
     6601 ggagaaactc tttcttaata aaacagatgg ttttgccgaa gagtttactg attttcacag
     6661 ggtgttagag ggtcagatta actctctggg atcagttgaa gaggcagtta tggatcttgc
     6721 caccatagaa gcgggactca tttccgcaaa agagaagaga gttgtaagag ttgactcttt
     6781 gctaaacgaa agctgagata tatatgcggg gattcaacac aagagcaatt cacgctggtg
     6841 aagagaaaaa gataaacgat gcggttagca cacccatctt tcaaacatct aacttcttgg
     6901 cagatgacgt gaagtacatg gacgaaagct cggaagttct ttacacaagg gttggaaatc
     6961 cctctattgg agtggtagaa agaaagctat cggatctgtt cggcggaact ggcggcgtct
     7021 tcttctcatc tggaatgggt gccataacta ctgtttttct gtccattctc aaatccggaa
     7081 tgaacctggt gatctcgaga aatatctatg gaggaacgca aagccttatc tccgatctgc
     7141 cgggaatggg agttgagata aggagattcg atcagtcacg aattgatgaa ctggaaactc
     7201 ttattgacga taagacgggc attgtgtatg tcgagtcgat gtcgaatccc gatttgattc
     7261 tttcagacat tgaagggatt gctcgaatta ctagagaaaa agaagctgtt ctcgtagtag
     7321 acaatacgtt tttatctccg tataatttca ggccgctcga atacggggca gacgtagaca
     7381 ttcagagtct gtccaagtat gtgaacgggc attcagatgt cattgcaggg tttgccgcct
     7441 tcaaagacag tggtctggaa aaaagggtaa gaaaggtaat gatcaaattg ggtacaaatg
     7501 gggcgccctt cgacgctttt ctggttggcc gcggggcgaa gactcttggc cctaggatgg
     7561 aattgcacaa cagaaatgca agagaagtgg cagaatttct ctcgaaaaat ccagaagtac
     7621 ttcgggtttc ttatccttcg ttgaaggcaa gcgtaccggc ctgtttctct gaatgcaggg
     7681 gatttggagg cgtagtttat ctcgaactgg aaagtttgag tgcagctaaa tcgttcataa
     7741 gaaggagcga gctgtttctt gaagctacta gtcttgccgg tgttgagtct cttgcaacca
     7801 ttcccgtctt gacaagtcat gcttctttgt cttccgagca gttacgcgat gcaggtcttt
     7861 cagaaggagg tgtaagactc tctgtcggca ttgaggatct agaggatctt ctgtccgacc
     7921 tagatttcgc tatgaaggga atttagttgg cctcgtcgca gctctttgcc ggaaagaacg
     7981 gaagctgaga aataaaggaa ctgagcgtga gaagaagcaa agaaaaccga aatgcaggaa
     8041 taagaatata aacggctttc cttatgaagc tgttggttag actgcaattc aacttacttc
     8101 ttcacccgtc tataattgtc aacaagctgt gaggtgatat gaagtgctga aaataagggt
     8161 ctctagtatc gaggagttcc ttctggcgga cagaaaggaa ttcaaaatgg aggcaggcaa
     8221 ttttgtttat atatacgact tcgagagact taccgattcg ccggggattc tcctccagat
     8281 ttcagtagtg aatagatctg atgctccgaa gaaagtcgga aagatagatc ttcttgaaat
     8341 agaggacgtt agcgaacccg tatattacaa tggttggcaa tcatggctac cattcaaggc
     8401 ttactgggaa caaccgattg tggatccttt cgttcagtac gccgattcac atgagataag
     8461 tctcttctca gcaacaccta ttcccgaaat cttgagggag ggaagaagac caagcgatta
     8521 cttcattgcc actgatgagt tggttgttgg atttctatcc agcagattct cgcatcaata
     8581 ctttctctgg aacgacgatc agtgcaatat acgcggctcg gcagatctct ttggggctga
     8641 actggcgcca ggtgaatcag ttcagctcga gaagatggtg atttttggca aagacagttt
     8701 gtggagtatg ttagagaatt atgcaagtct tatcaagaaa agcaacaagg tcgagttcaa
     8761 cgatttcagc ggtgtcgggt ggtgtagttg gtatcaatac ttctcagaca tcacctttga
     8821 acaactgtca aagaatattc ggctgctcgc ggaaattaga gatcgagaga aaatcgatta
     8881 tagactggtt cagcttgacg acggatacca ggaggatatt ggtgattggc tcgaaacaaa
     8941 ctccaaattt cctgaactga aggaaatcgc ttccgaaata agaaagagga aattttcggc
     9001 gggtctctgg attgcaccgt tttctgcgtc tgaaacttcg aaagtcttca gggaacatca
     9061 ggattggttc gtgagaaacg aagacggctc accgagaatt gcctacagga actggggaaa
     9121 gaagatattt tccctggata ctacgaatcc cgaggtgctt gaacacctga gagatttgat
     9181 tgtctcgata aggagatctg gttttgatta tctgaagatc gactttcttt tcgccggtgc
     9241 cattcccgga aggagataca gagaggactg cggtccgatc gaggcttata gaataggcat
     9301 gaaggctata agagatgccg ccggaaaaga ttgcttcatt cttggttgcg gcgcgccgct
     9361 tcttccttcg gtaggatttg tggatggtat gaggattgga tctgatacct ctccacaatg
     9421 gaacggtgaa gctatggata tcggtattcc cagtgcaagg ttttcactaa gaaatgctta
     9481 tacaagagca tttatgcata gaagactttg gcttaatgat cccgacacgg tcatattgag
     9541 aaagtgcgaa atgactgaag aagagaagag gatcttcgcc ttgtccgtgg gactgcttga
     9601 tggaatgatg ataaacagcg acgatatgga actggttgat tcacagggga tctcacttct
     9661 aaaagaagcg ataatgctaa aaggaggaga acctcgtgtc ttccttgacg gaatgaatag
     9721 tgtttttgcc acttgcacta gaaaggccaa ttactgcaat gtgacttcat ttgtcaatct
     9781 tactgacacg cctagacact ccataaagct caaagagatg tatgaaaatt ggtctggagt
     9841 taagcctccg gttaaggaag tcgagcttcc accaaaaagc atatggatag caaaaagtga
     9901 ttcatgtgat atctagtacg aaaattatat aaagtaacaa cgggaagata ccgcgaactt
     9961 caaatgaaat cagttaacgc tgtctcttgg agtaagaatc cacaccgtta ggggaggaac
    10021 tttgatctcc ccaccaaaaa atgaaatagt ctctccagtc aagtattctg tgaattcacc
    10081 ttccatcgaa gggctgaaga atgcagcctc tcgtgaggga ttggttatta ctattacttc
    10141 gtcagtggcc ttccatcttc taaaagagaa aaccggtcct tcagcggcaa tgacctcata
    10201 ctcgccagtg cggatcgatt cgtgcgatct tctaagatct aacaaagcct tatagaaatc
    10261 gtgaacatca tgattccagc gctcttcatc ccagatcatt ggcgctctgt tttcagggtc
    10321 acgacctcct ttcattccta tctcatctcc atagtagatc actggactgc caatgaacgt
    10381 catctggaga gccacagcga tcttcataag gtcgatctga ttgaaagcaa gagtaaggat
    10441 tctttcggtg tcgtgactgt cgagaatatt ccatagagaa tgaagagtct ggggcggata
    10501 agcattcagg tacagatttg tcatagcagc aaacttagct gcggaatttc cggctctgaa
    10561 cacgtattct acaacagcat ccttgaaagg ataattcatg acagaatcga aactcttacc
    10621 tcttagcaga gatctagcgt caccccaaaa ctcagcgaca agtatcatct catcgtctaa
    10681 gcttttcaaa agtggtctga aaaagtcggt ccagaatagt gggtctactt ctgttgcgac
    10741 gtctaaacgc caaccgccaa ttccgtcgca atcgaatttc cttaccagtc tctcaacgta
    10801 tgattgccaa tctttgttaa gagcgtttat tttcggcata tatgcgtagc cgttccaccc
    10861 aacatatgtc attgccctgt cctgaacttt tcttatcgga aagcgtttca caaaatacca
    10921 gtcaacgtag ggagattctt ctcccctttc tcttagatcc ttgaaggcga aaaagtcgta
    10981 tccagtgtga ttaaacactc cgtcgagaat gatctttacg tcgctgtcat tggcagcggt
    11041 tagaagttcc tcgaagagct ggtcgtctcc gaaattgtca tcgattttca gatagtcttc
    11101 ggtgtcatat ttatggcttg aggggctctc gaaaatggga ttgaggtata ttgcatctac
    11161 ccccagttca agtaagtgat cgaaatgatc caaaatccct ttgagatcgc caccaaagaa
    11221 accgtctcct ccaagatttg cgtaatcggg atcggcattc cattcctgaa cgtttggcgg
    11281 atcatttctc gggtctccat tggcaaatct ctccgggaat atctgatagt atgttgctcc
    11341 ctttgaccaa tcagggaggt cgaagtagga aatcggaagg ctctctttcg aaaaacagta
    11401 gggaattcgg ggttttctgc agagtccaag agagtcgaga aagacaactc cagaatcggt
    11461 ctgtatttcg aagtagtatt caagttcttc gacgcttaat ctttctgtgc ggtagaagct
    11521 ctgatttcca aagtcccgta gatattcaag cgggtagcgt tccccagcaa ttacgatttt
    11581 ggcttcaaaa gggacatcgg tcggagccga gactgcaaaa tagtattcat catggtttat
    11641 cgggtttaaa taatccctgt ctccaacgct gtgtctgaca gaatcatgta ggacctcgaa
    11701 atcctcgaca atcatcacag acatttctct ttcattctcg taagattttt catcgttcct
    11761 ttcatctggg aaactcgatt cctctgtggt gaaatagtaa cggtatctac caggttcaag
    11821 agaaacctct gctgtccacc aatcgttttc cagtctcatc atttcgagtg tcattgattt
    11881 ttccataact actactgaag gtgtgctctc cgatgtggtc aagaaagata ctccgcatcc
    11941 taatgtcata accgttaaga aaaggatgat catcattttc tttactaaca tcattttgac
    12001 tcctcccttg tttttcttct cgccaaggca ttggcacccc aaactcccag aagaattaga
    12061 gatatcccaa ttacctgaag tggaccgaat ctctcgcctc tgaagacaac tccggctaaa
    12121 agagatacga cagtcgacag ataaacgaat accgttgatc gcgacgcttc aagcttagaa
    12181 agcatgaaat tgatgaggaa aaaggctccg accgatgaca aagtgcccag ataaagcgct
    12241 gctatggcaa cctgaggtgt acccaagcca aaaatgtaga aagagaactc tccttttatc
    12301 aaagatgtta ccacagcgat caaagtgaaa gagatgaaac ccatagtcat catatgatat
    12361 gttatttccc atgctttaaa cctgacagaa gcctttcttg acaaaatatt gtaaaaggca
    12421 gctgaaactg tgctacctaa aagaatgatg ggtccaagga cactgccgcc cgttgaaaga
    12481 cccttcccgg ctgttatcag aactattccc agtagcgcgg tcaagactga gaacacttga
    12541 ggagcggcaa gtctttcctt aagaaagact ctggcgaata taagaacgaa tatcggaatt
    12601 gtcgaaagca ggataccagc ttcggacgat gtcgacagtg agactccaac gttctcgaag
    12661 atgaagtaca tgaccggttg aacaagactg agcaaaagca aatccttgaa aatagacttg
    12721 tatggcctta ctttgattac acccaacgct ctcaggagag aaatcgtcac gaaagcgact
    12781 gaaaaccggt acgaaagaaa gtacatcgga tttgtatatt caagagcgtt tttcgtaaac
    12841 aagaaggaaa gaccaaatat caccgaagaa ccaatgccag caaaataggc cagaagtctg
    12901 tccctttctt tctctgccat aaaacacctc aatcaataat atcaccggga aaaacaaacc
    12961 ggcaacccga gaacgttgtc tgctacacat agtctataat aatagtagtt gtgcttcgtt
    13021 ttcagagggc ggtggctttt tttgttcaga agatatttct tgataacatt tctcttattg
    13081 tcggcatttc tttccgggta ttcatttagg gatctcgact ggttgaagca tgagtccgat
    13141 cactttgtat acttgtacca taaggaagtg gcttcctcta tttttcaggt tgaggcgttg
    13201 gcggaagatg cctttgatac tctaagtgag ttgtttggca atccgtttaa agagaagata
    13261 actgtgatca tcggtggcta tgaagatcgt tctaatgggc tggcaaatcc agttcttgaa
    13321 tcgatttata taatgacagt tggaatggac tacccatata gaaacgacgg tttttggctg
    13381 gaagaggtga tcactcacga actgactcac ctgtttcaga tcacttcggt cactgaattt
    13441 ggaaacatac tgagaaacta tgtctccaaa gcctatttgc ccaatgcact tcagcctatg
    13501 tggcttactg aaggctttgc tcaattggga agtgaactaa tcggagatct ttacgagtac
    13561 gactacaggc gattgccttt tctgctagat caattaatgg aaaatgagcc attttcggag
    13621 gagactgtag taacagggtt cagcggaata ggcggagagt cttactacaa ttacggctat
    13681 gcttttcttt cttatctttc cgagacttac ggaatcgaaa gcttactgga acttgtgaaa
    13741 gtgaagaggg gacttcttgg tctcacaggg atagagcttg cgataaagaa ggtatatgga
    13801 aaaagctatg aagagcttaa agaggagttc atagaatatc aaatcaagaa atgggaagct
    13861 gcggaagtac ccttcgttta cgaattctca cgtggcgttg gcgaattcgt caatcatttc
    13921 agaccaaaga tttatgacgg gaaactttac tatctttctc ttgataggca gatgaaatgt
    13981 tattctctca acatcgaagg cgttgagata tttaattcta caatggagat tgtggatttc
    14041 gcagtctttg aaagcgaaat agcgcttatc ctctttgaag aggagcaatc cgaaaccaga
    14101 ttgtatttct ttagggaagg caagttagag agaacaaaaa cggctcatct tcgtgaaatc
    14161 gattatttgg gcaaggatag gcttcttgtt gtgagaaaca atctcggcat ttcttcgata
    14221 gaaatccttt ctttgagaac ggaaagggta accaggatct tcacgccact tgatccttcg
    14281 actcaaattg acaatataag agtatcttgt gacggcagtt taattgcatt cagaattaac
    14341 aataaaggcg acaagtcact ggcgatttat aactcaagca ccgaaaagtt tggatttttt
    14401 gacgttacga atgatttctc gattggtggt tgggtttccg agggattttt aatttctgtt
    14461 aagaacgagt tcggcagcga tgtttatgtt gtcacatctg aaggtgagat gatcggaaag
    14521 agctccttct caagatatgt tagggaacca gtaatgattg cagacggcgt gattgccgta
    14581 ggccagcttc agggaaggaa gctgctcttc tcctccaaca caaaaccaac cgattatagt
    14641 ttggagagca aagaaatcat tttgagtcct cctcaagaaa tagagggagt cgcttacaac
    14701 ggcttttcag actggagatt cgtttcgcta cttcccttta aggggatttc ggtgctcttt
    14761 tccgacctaa cttttcgcaa caagcttctt ggaggggtgt cgttcgactt cgagacgatg
    14821 ttgcctggaa tcaatgttca actcttcagc gaagactatt tgccggtgga tttctctctg
    14881 tcgctcgaat atgccaatct tgagccgaaa gctctgttaa acgtttacag gcgctacagg
    14941 attgatccca agctttcttt cggttggaaa gcttcgcttg agtatccttt gagaattgcc
    15001 ggttccctcg acgcggctct tgaggattcc tcagcactgt acggaggtca ggcctcgctc
    15061 aatacactga taaatgttcg agacacacag acggcgactt ctaatctcgt agatgttgga
    15121 ataaacgtag acttcgattg gaataagcac ggatttacta tagattcgac aatttactcc
    15181 agagttactt tcggggaaga gccttccata gtcccggtgg aactttgggg ctttagagac
    15241 gacactaaca ttgttgtcgg cttttccctg gactcggatt atgtcatttc gagaaggggt
    15301 ctcaatgtga tgaatctcgt ccatttctca gaggaaggta ttgggggaaa gatcgacttt
    15361 ttgatcggta gtcggttttc ctggagaata gctatctaca agtttgaaac agccttcctg
    15421 tacgcagcct atcccatgca cataaaagca ggaatcatgc ttgaaaacac tagactgctc
    15481 ccttatctgg attttgagct aatatattag aaactcacaa ccatccgggg aatctataat
    15541 cgatctttta taaagctttc ttccatcgtg tatttcgaac tctatccttc tttcatcaca
    15601 cttgaagcta ccttcttggg gtttgacttc aaccttcagc ccgtttgaag tattatcaac
    15661 tatgattgtt tttgatgaat actcgccttt cttatatgcc tctgtgagac catcatcctc
    15721 atataggtag aattcggccc tatccttaac aaagcccaag aacttcagag ttttccagga
    15781 gccgcttttc acaaattgca gaccgtccgt catggcaacg agcgagtctt tcttcaaaaa
    15841 gtaaggaatg atgttgagag gtgcttttgc ccatgaatga ccgctgttga ccacttttct
    15901 ggtgttgaga tcaagccact cttcgcctgg aagataaaca tgccttcctt cggaatttct
    15961 ttgataaacg ggagctacca ttatcgaagg accgatcatg aactggtcgt cggcataata
    16021 cgtttcgcta tcgccgggcc aaatcagagc gagcggtcta accatagtca tctccccgtt
    16081 tgcagattgt ttatgtaccg aatacaggta tggaagaaga gaatagcgta gctcaatcgc
    16141 ttcccttaca agttcctcaa cttccttgtc gaaggcccag ggttcctgat tccttgtccc
    16201 tatggcgcta tgatttctga agaaagggag gaaagcccca aactgtgtcc aacgaactag
    16261 caattctccg gagcaatcgt caccgaaccc tccgacatcg catccggtga aactaactcc
    16321 tgctaaggag atagactgaa tcagcctaat ctcattcaga agctgctccc attgacttgc
    16381 attgtcacca gtccacagga ctgcataacg ttgtattcca ggatacgaag acctggttat
    16441 attgaacggt ctcagatcag acttgctctt cctgattcct tcgtatgcgg aacgagccat
    16501 attgaagcca tagatatttc ttacctctct atgagaatga gtgttcccat cgtcatcctt
    16561 ttgaacgaaa tctactccgt gatcgtaata cttcttcttc gaaataatct ttccaaaaag
    16621 gaattcagtt tcgattccag aatcatgaag ctcttcggcc ataagcttaa gttctaaaag
    16681 agattccggt gtatagaaga tagcaggttc gttcatgtcg ttccagaatc ctgagactcc
    16741 attctttata agacgatcat agtgttgacc ccaccactcc cttgcggcag agtttagaaa
    16801 atctgggagc ctcgattctc caggccagac agcagctcta aatgggctac cgtcctttct
    16861 caaacagaac aagtctttct tctgcccttc ttcgtaaata tcaaatccgt cctgagcttt
    16921 gacccctgga tcgattattg caacagtctt gatccccatg cttgacagat tagccatcaa
    16981 ggaagaagga tttgggaagc gatttttgtc ccacgtgaag accttgaaat catccatgta
    17041 atcaatatcc aaatgaattg catcgcacgg aatgcggcgt ctcctgaaat tttcagcaat
    17101 ctctaaaacc gcttcttcgt ttgcatatga ccatctagac tgatggtaac ctaaggacca
    17161 ggcaggaaac aacaatggat tccctgtgag cttgaagatg cgtctaattg tatcagaagg
    17221 actcccaacg aaaatgaaaa gatcgaaacc gtccccctca atgcctattt tgattgtgtc
    17281 tctattttca aatccaatgt caaaactcga ataacctgga tggtcaagaa aaagagcgaa
    17341 ctgctcttcg ggggaaacaa tgtggaaaac gggaagagat gaatacagtc tcctctttga
    17401 tggcgagtga tcaggttcgt cggtattgta catctcatag atactgcctc ttttgttcaa
    17461 aggtcccatt gtctctccta gccctaggac agcctggttc ttctggagcg tgatgttcac
    17521 aacataaccg gcgtcattct cagcgacact catctcatta ccggctcttg aaacaaatgt
    17581 tctagcctgt ccaagatcta atacgcctag aagttgacat gcacccagct cgtcaagttc
    17641 ctcttcgggt tcaattatca ccgcgccgct cttcagcttt gtctttccct ctctcacaac
    17701 actgacctta acaacagaat tcaaataacg aaagaagcgt ttcttcaatt cgtcaccccc
    17761 ggttcttaga aatatcgttc tcattgtaca gcaagatggt agactttgta tgtattttga
    17821 aaagcaggag gtagatcgat gaccgtaagg gaggcagtct gtaagataaa gagcctgaga
    17881 gccgaaaggc tttcagaaat cagcggcaca tatatgtttt tcatcgcggg tggggaacca
    17941 tcaaaattcg ctgtcgaaat cttggatgga agacttgaag tgtacgagga agacaaacct
    18001 tcggattgtt ccataagtac tgatcttgaa accttcgaag gaattgtcgg aggagctgtc
    18061 aatccttttg ctgcttttat gagcggaaaa ctcaaaattt ccggagatct gactttcgca
    18121 atgaaattga acaagctact tggaggtgaa tagatgtcgg ctgaagtgat tatacttgcc
    18181 tttgaaggca agaaagcttg ttttttacac gcgctcctaa atgcactgga catgtctgaa
    18241 aagggaatcg aagtgtctat cgtgatggag ggagaatcac ccagtctctt gaaggaaatt
    18301 gtaaacgaaa cggatgctct acatgggctt tacagcgagg caaagaggaa aggtttgatc
    18361 aaggccgttt gttatggttg ttccaagttg atgggtgtgc ttgagatagt ggagaaggaa
    18421 ggtcttccct taaatggaag tatgaagaac catgtgcctt tggctcctta tgtagagagg
    18481 ggagcggaga taattacctt ttagtctatt gaacgcagct tgcccgtgac accacgaagc
    18541 agagcagatc agaatatttt tttcttggga aaggagtcct ttatgagaaa cagtctaagg
    18601 cgttggaaga acgagaaact ctctgaaaga cttgcgaaag ttttcgagag taaggcgata
    18661 aaattcatgt acttccccga taaagagatg gtcgtagatg cggtttcaaa gctcgttcct
    18721 gacgaagcaa ctgtgagtgc gggaggttcc cttactcttc aggaaactgg ggtatccgac
    18781 cttctgaagt ctggaaagta caactttctt gatagagatt ctgtttctgg taatgaggaa
    18841 aagaagagga taatgatcag tgcgtttgag gccgattatt atttctgcag tgcaaatgcc
    18901 ataactgaaa gtggcgagat acttttgctt gatggcaacg gtaacagggc tgccgcagtt
    18961 accttcgggc ccgacaaagt gattctaatt gctggagtta acaaggttgt cagcgattta
    19021 gacgctggaa tcaagaggat tagatcaata gcaccgatga acgctaaaag attgaatctg
    19081 catactccct gtgctgaaag cggtctctgt accgactgcg acagcgatga aaggatttgt
    19141 gaaatatact cgataattgt tgattcaaga agaagaccct ggagatacac agttattctg
    19201 gtcggtgaag aactgggctt atgaggacgg taaggattag agtctacgga cgcgtacagg
    19261 gagtggggtt tagatattac gctctgtaca ccgccaggtc tctgggcata actggctatg
    19321 tcaggaacga acccgatggg agcgtcgaga tagttggctc tgggagagat cccgatattg
    19381 aggcttttat tgaaagcatt gatcggggcc ctacgtatgc aaatgtaacc aagacggaaa
    19441 ttgaagagct acccttcatg agattcgagt attttgaaat cagataatga agttcagatc
    19501 ccaccccatt ctgaaagaaa ctgtttcacg aattgcacga caaatctgta gcggttttct
    19561 gcgatttcct ttgcggtctt cgtccagaaa ctctccggag ttatcttcaa tatcttctca
    19621 aagaaatgat taatggcagt acttgagtac cctttgtatg aatcacctgg cttgagcttt
    19681 ggtgaatgaa gaggagttcc cgtttttccc gaaaatgaaa aaacccttgc gacagcaata
    19741 gcacctattg catcgagcct atcggcatct tgcagtattc ttgccgacat gctggaaggg
    19801 tttaatccag aagaatatcg gtgcacctta attgcttcag agacttcgaa agtgaattga
    19861 gattcgaaac ccatctcatt caaaagctga aacgcatatt tcgctccgct ctccgcatga
    19921 tctattccag tgaaatcctc ttcgggtcgc ttaatatcgt gaagaagtgc agcaatgacc
    19981 gcaatttcga ggtttgcccc ttcacggatg gctatttcac tcgcactttc agttactcgt
    20041 agaacatgag agatgtcatg agccgggtcg tcgtaattct caaaagcctt ctcgacaacg
    20101 cccaaaattt cttttatgtg cggtatgcag ttgatgagtc ggtcaaagtc cacaggatgc
    20161 cccctgagtc aaaaagaaaa gttgcgctca atattgtaga ataagtatac aagatgatta
    20221 gttcgaacag ttaagaggag gtacaaatat tgaatatcct ggttacaaat gacgatggaa
    20281 taatgtcgcc cggaattata aaactcgcgg aagcgctttc ggaagaacat gatgtcctag
    20341 ttgttgctcc agacgttgaa agaagtgcaa ctgggcatgc aataacaatc agaaccccgc
    20401 tttgggccaa gcaagtaaaa gttggcaaca aaaacatcgg atatgccata aacggtacgc
    20461 ctgccgactg tgttaaactt ggacttctgg caatttccga aaagaagatc gatttggtca
    20521 tcagtggtgt aaacaagggt cagaacatgg gtatagatgt cctttattcg ggaacggttt
    20581 caggtgctct agaaggcgcc gtaatggata ctccatcgat tgcagtttca agtagtgact
    20641 ggagtaatcc ggaattcgag actgccgcag cgtttatggt aagttttctc agagtctttg
    20701 atgttaagat gatgccagac ttcacggccc tcaatataaa tgtcccttct gtcaaatctg
    20761 aggagctcaa gggttggaag gtgacgaggc agagcagacg gagatacaag gactatttcg
    20821 aaaagagaaa agatccatac ggaaataact actactggat attcggagag gtagttgaag
    20881 atgagtgctc aaccgattgc gattataatg ccgtaaagtc tggattcgta tcgataaccc
    20941 ctctccacgc aataatgaca gataatgatt actttgagaa actgaaagag atttcaagag
    21001 ggtggtaaga gtgaaagtga tttacatagg aaatcctata ttgagagatg tttcggagaa
    21061 ggtagaggta ttcgatgatg aactcagaca gttcgtgaat gaactgagta agacgatgta
    21121 tgtagaagac ggagttggtc ttgcagcacc gcaagtagcg atatcgcgaa ggctcttcgt
    21181 ctacgacccc ggcgacggac taagggttgt agtaaatccc gagatccttt ttagaagcga
    21241 tgagaaggtc aagatagaag aaggttgcct aagcataccc ggaatttacg cagatgtagt
    21301 cagaccggcc tccgttcgaa ttcgttatca ggacgagtat ggtcattacc atgaagacga
    21361 cctcactgat tatcccgcaa ggatagtaca acacgaaacc gatcatctgg acgggatact
    21421 cttcgtcgac tatctctcgt cagcaaaaag ggcaatcttg aaacccaaac ttgatcagat
    21481 aattaaggaa agcgttggat agatgaatat tgtcttcatg ggtacgccgg agtttgctgc
    21541 atcacatttg aaagcgcttg ttgagtcagg tgttaaggta ataggtgtct tttcgcaacc
    21601 cgacagacct aaaggaaggg gacgaagagt ctatcctact cctgtgaagt cagttgcaga
    21661 agtctacgga ttgccagtct ttcaacccga aaaagtcaat tctggagaag gattggaaaa
    21721 actcaaagaa ctttctccag atctaatagt ggttgttgcg tacggaaagc ttctcaaatc
    21781 aagtgttatt gaccttccaa ctttaggctg tttcaatgtc cacgcctcac tcttgccgaa
    21841 atacagagga gccgctccga ttcaaagagc aatcgaaaac ggggaaacga ggaccggtat
    21901 aaccatattc aagatcgacg aaggaatgga taccggcgaa atagctttga ggagggagat
    21961 cgagatagag atttcagaca attttggttc gctctatgaa aagcttgaga gactcggaag
    22021 ggaagctttg cttgatttct tgaagatagc tgaggccggg agaatcgagc tttgtaaaca
    22081 ggagggagca ccttcttacg cgccaaaggt ccaggcgtcg gacctggtca tttcggattt
    22141 ttcggatgca gtttatgttt tgaacaaagt gagagcttat gatcctcaac ctggggcaag
    22201 aacatccctt gacgggaaga tggtaaaact gtttggggca gttttttcag atcgaagtta
    22261 tggaaagagc agggatggag aaattgtgaa agtggaagac cacggtatgg ttttaagctg
    22321 tggcaccgga aatgttatta tatcgttagt acagttaccc ggaaagaggc cgatgagccc
    22381 taaagatgcg gtaagtggcc ggctgattcg tgaagggatg attttggggg gatagaaatg
    22441 cccagggtga tcaggctatt tcaaattagg aacgagatta cggattccga tgccgaacga
    22501 attgtttcga gactgaagtc cctcgacggc ataacaaagg cccaggttga tgttgcaagc
    22561 agtgtcgttg aggtggaata tgagaacgcg atcatcgaca gatacattat tcaggaagag
    22621 ctgtccaagc tcggttttga tatgcttata taaaccattc tcgcgatttg cttcttgaca
    22681 aaggaatccg gtcaagttat aatttctgac gtgtgacgtt gccgaggtgg tggaattggc
    22741 agacacgcat ggttgaggtc catgtgggca gtttagccta tgcgggttca agtcccgccc
    22801 tcggcaccac aaaaccgggt tttagcccgg ttttttcttt cgaggtgctg cttgggagaa
    22861 attctacttg gtaaggtttt caaaaggaac aatgaaatta agataggctt gaggcgtctc
    22921 tttgagagcg cttcatttgt ttccattaat ataaaggatc ttccttcctt tttttcggaa
    22981 agagacgaag actgcctctt catagatgct tctgtagagg aggtgtatct aagtgagatt
    23041 gccgtagatg aaaggttttt gaatagtttc gtctaccaac cagaatccat agatcttgaa
    23101 agccgtatag ataactatat aagagtcatt aagaagttgc cgattggact ctcatcatta
    23161 atcgagaaaa tcgttcctga cagaggactt agaaactttc tcctttttgt gttaaagcag
    23221 agtgagaatg tcgatccttg ggaagacttc ttactctcgg ggcttaccag gagtgaaaga
    23281 agagaaagcg ggaattccag aattgacatt aagaaggcag tcaggcttgt ctttagtgag
    23341 ggggggcttt tacaggattc aatggaaggg tatgaattca ggcaggagca gttcatgacc
    23401 gctctcgaaa ttgctgaatc tgtagaaaaa ggtggcaatc tcatgattga ggttggaacg
    23461 gggactggaa aaagcatagc atatcttgct ccagtagcat atgcttgtgt ttccaagatg
    23521 catcaagcgg tgatctcgac tagaacgaga atcctccagg atcaactgtt caaaaaggat
    23581 gtggaggttc taaagacctt accgaacctc gataatctca gagtgtctgt aattaagggg
    23641 agggaaagat atctatgtgt cagaaagcta tttgaagttc tgaacatggc tctcaataag
    23701 atgctcgatg aaagttcgtc gaaagaactt gccggtgtga ttctatggtc aatgttcacg
    23761 agagagggag atcttgatta cttaccgttg ggtgatgagt ttagaaggat gctctcgggc
    23821 aacaggttcg attgcaccag aagactctgc ccattcttcg aggtttgtcc ttactatgtt
    23881 caaagggaaa atgcaaagaa ttccgatata gttatcacga atcattcatt cctgttcagt
    23941 gaagcgaata ttcggctttc cgatggagaa ccggaagacg gcgaaagtat tggaacactt
    24001 ctaccggcat ttaaatacct tgttgtggat gaagctcacg agcttgagtc ttcattgact
    24061 caggcaatga gttaccggtt tgaaccatat cagctagcta ataccgttaa gaaactgata
    24121 aagctggtta gagactgctt tggatttctc aagaggcatt tcagcgagga ttttttaagg
    24181 aagttgtgga ttaagcttaa agaagctaca actactcttg acaggaaaat aagacagctc
    24241 gtggtggatc tggataggga acgtaatggg gagaaaacta cgtattctgg aaaaggactt
    24301 gaaacgcttg tctcagcact aaccgtaatc tcagatattc ttcagacgca tatctatgtg
    24361 ggcggacaaa cagtacaaat gctggatgat gttgaagaaa gggacttgaa gattgacggc
    24421 ttagaatttg aagttgctag ggttctggct gaaataaaag aatggcttaa gcagatcgcg
    24481 ggaattgccg cttctagaga ttctagggta gtttttgtga gcaggaacga acaatgcatt
    24541 gaaagcttga gtatagtctc ttctccagtc gaaaacgaca ctctgatgtc ctcgattttc
    24601 ccggatgtgg gcgtaaagat cttcatatct gcaacactgt gggtctacag cagaggtagc
    24661 gatggtttca actatgcgag aagaatactc ggagccggcg aagagaccga tgccgtaagg
    24721 cttggaacct cattcgatta tactcagcag ctgagatttt tcgttcccac agacattccc
    24781 gactatctgc caaattccct tccatacatg caaagtgcat cgaaactgac aaaagacgtt
    24841 ttgaagatag tcgggggagg gtccatggtt ctcttcacct cttacgaaga catgaaatgg
    24901 actctttcga agattcgagg tgaacttcac aacttgtctc taagaatcca ggaaagagac
    24961 gatagcttaa caacaattct ttctgagcac aggaattcag agaaatccgt aatatttggt
    25021 gcccgagcat tttgggaggg aatcgatctc cccggagaac agctgaagct gttgattgtc
    25081 tacaaactgc cttttgaaag gccggacgaa ccactaattg aagcaagaat caagcactat
    25141 ggaaagcgga gctttgccga aggaatgaac aagtattact atccaaaaat gataactgcc
    25201 ttccggcagg ggcttggtag acttataaga accaggaacg atcgaggggt tgtaatcgta
    25261 cttgacagga gaatcatcga tccggcaagg gtatattcta agaagttgat tgcatctctc
    25321 cctcctggaa cgaatattca agcgataaac agcaagcaga tacttcgaga gcttagaaga
    25381 ctgaagaaag agagatggct gtgatgatga agaagctgat ctctcatttt ttcccaaacc
    25441 actgtctagt gtgtgaaaag gagatagatc cagcccagta tttgtgtgca gactgtcttg
    25501 gatcaatcaa ggggccaatc atgactccat ttgagcttaa atttgttgat tcggcctttt
    25561 cgtactggaa atatgaaagc ccattaaagg aactcatacg tgcctataag tttgaagaca
    25621 gacccggaat agcctctctc ctttcagaga tgctcttcga aatgattaat acgttttctc
    25681 cgtccgccga tgtgatagtt cctgttccca cttccattca agcatttgtc aagagaggct
    25741 tcgacacaaa tctcagaatt ctgagaaggc tttctaagac tattgacata agaatagaca
    25801 atgtgttgac aatagcaggt aagtctattc ctcagtcaag gcttaagatg gaagatagat
    25861 tccagaatgt aaaggataag ttctatctga agagaagtcc taggagtacg tcagtaattc
    25921 ttttcgatga tgtcgttact actggcgcaa ctgcaagcca gtgtgctaga gttcttaagg
    25981 aaggcggcgt taaggaaatc actttgctgg cgattgccgg ggggaaaaaa taagggcctt
    26041 gcggcccctg gctgggagag gaggactcga acctccatcg gcggatccag agtccgctgt
    26101 cctaccaatt ggacgatctc ccaagacagt aaagattata aagcagaagg taagaggtgt
    26161 caagccgttt ggaggcatag gatgaacata gcgatgttca gtgacacata ttcacctcag
    26221 gtcaacgggg tggtgaccat gatcaagatg cttgaagaga atcttcagaa gagaggccat
    26281 aacgtatata tatttacggt tgaccacccc gaagcaggca tccaggagaa tgtttatcgt
    26341 ataccatctc ttaaatttcc atgggaaaag caacaccgaa taggtttgcc cacaaacttc
    26401 aaggaactaa taaagatcgt gaagaatctc gagatagaca ttattcactc gcacacgtca
    26461 ctaatcgttg ggtatctggc gagttatgtt ctaacgaatt tgcacattcc aggagtaacg
    26521 acttatcaca caatgatgga agagtacgtt cactacatcc cattcatgga acccattctg
    26581 agagtttaca tcagagctca ggacagaaga ttttgtgata agcacagggc tgtaattgct
    26641 ccttcgataa aaatcaagaa gctgcttctt tcttatggag tttcttcaca tatagaggta
    26701 attcccaacg gggtcgatct tactccattt aagagaagct tcagcagaga agagaaaaga
    26761 gcattcagga agaagtacaa gataaaagag gacgataaag tgctcatctt tgttggaagg
    26821 cttggagaag agaagagcat agacaaactt atagagaatt tcgcaagagt ctccgaagct
    26881 ttaccagact cccatttgct gctagttgga gatggtcctc tgaagggtaa gctgcaggaa
    26941 ttagccagaa gtctgagggt cggtgagaag gtccacttca cgggattctt gagatggccc
    27001 gacgagatat cgttagccta caaaagcagc gatgcattca tgatcgcttc tcacactgag
    27061 actttcggcc ttgttaccct tgaagcgatg gcctcgggat tgccggtcgt ggcatataag
    27121 gacgatagca tagtaaatat ggtactggat ggagaaaatg gcttcatgtg ctcctcgaaa
    27181 gatgagttat ccaatgcggc tattcaaatg ctgagtgatc atagcctaag agaaagaatg
    27241 gcaaaacgct cagttgagat ctcggaggac ttctccgctg aagctaatgt agagagaaca
    27301 gtgaatcttt atgaaagagt tgtcaagaac gagatctaga tttgaatcct atttcatgag
    27361 ctgaaagtaa tcctccgaaa atgatgtttg cgtagtagct gaagaatctc cagatcagaa
    27421 gcgcagctcc aattccttcg ccaaaaaaag acttaaaaaa tagcacatac ccaccctcag
    27481 atactccact cgctcctggt gttggaataa gagagactac aaggaacaaa atcagctgaa
    27541 cggccgtgat ttcgagatag tttacaggcg ttccaattga aagggcaaca aaatacggga
    27601 tggatatcat gcaaatcagc tgtgcaagag tactcaaaga cgcactagta aggacaaagg
    27661 gtctcctgac caattccttc attgaagaat ggaagaagcg tagctctctc attgccttct
    27721 ctattaacga ttctttctct tttactattc tgatcttgtg aagaaagctg atcacaaagt
    27781 ggatgagctt atcagttagc ttaggactga gcgaaaatag aacaagtcct atcagaacta
    27841 cactgttcag cgcaaatccg aaaatagaga gcacggcgag attagaaatc ttctgtgaaa
    27901 caagcgggta ggccttgaaa acggctacag ctccaagcaa tgtgattacg gtttgataaa
    27961 tcagaaatct tgaaacgagc atcgcagttg attcacctat cgagatacct cttctctgca
    28021 taaagactat ctgcgccggc tgtcctcccg aagagaaagg cgttatacca ttgaaaaatg
    28081 ttccaataac cgtaatctta aaaagaaata agaagggaat cgccagattc cgcatggctg
    28141 cgaacagttt aagggtgatt gcttcgaata accaagtacc gaacgttaga cagaatatca
    28201 gaaatatcca tccgggattg gctgcagaaa ggtaagaaat cgatttgctg aagtcggtcg
    28261 caaaaagaac tatcagaata atcagcaaac tgagagaaat cgcgatcata aagtttctgg
    28321 ttgatttttt cattttttcc tgtcaagtac ctctaggtag attttcttaa gttcctgacc
    28381 aataatcgac agatcctttt ccagcgcagt tccctttcca catgccgata gtttctttct
    28441 cagactttca ttgtgaagta atctatctat gttctccatg aactccttgt tgttttttgc
    28501 cttcagacag ttctcttcat gtttcatcca accactgtat acaggtatat ctctcaaaac
    28561 gatcggggct tctgtagcca gggcttccag gacaacaatg ccttcagtct cttcatagct
    28621 cgggaaaaga aagacatcac aagccgaata agggccgata atcatctctt gcggaatgaa
    28681 cccgggaaat ttcacattgt ctggaggatt tttcaaaagc cttcgaattt tggaaggcag
    28741 aagactgctg atttttgctc cgaaccagta aaaaactaaa tcagtcctct tctcagctat
    28801 ttcgcagaaa tctattactc ctttcctttc gaaagggagt ccaactgaga caatcagggg
    28861 ctttcgaatg ttgttttcca aaaggaattt ctcggccagt actttgtttt ttgaaaatct
    28921 tgtattgtcg acgccatttg aaacggcttt gattgggaga tcgattccat agctctttat
    28981 gagatttttg gcatattccg taggtgaaat aagaaaatct gctgatgaat agagctttat
    29041 caatcttttt ttcagccagg gtgcgatcgc gtttgacaga gtgaacgagt ttctaaagtc
    29101 ctcataggtt gtgtgtgtat gatagataac gggaatgttg gacttcttcg ctttcttaag
    29161 aatcctctcc gctccgggcc ccaccgtgtt tatgtggaca acatcaaaac ggtcttcctc
    29221 atttgttgta tactccacac cgactttctc aagagccgac atttggtgct tcaatgcgat
    29281 tcccacgcct gacttactga agagcttctt tccttcggaa tacaaaagga ctctcatttt
    29341 tacctccagg acagttgtta gtagatcttc gcagtcgatt tcgactagaa aatgcccgct
    29401 gaaatctata acaagaaagg attgtctaca aaaacgtcaa aaagaaaatc ttacttcttt
    29461 ccttatgtta gaaaacagtt cagacatgag tggccttctc actctcttca acgactattc
    29521 taccctatct aagaatattt atcacttgaa ctgcctccta tcaatgtttg acacactaat
    29581 cagagtatcc agggaaacta agtcccctcc tttgtttttc ttcaggctca gttaagggaa
    29641 actgaaagag cttacaagag tctaaatact ggaaaataac aattaagtgt tcagtcattt
    29701 tgaggaaatt gactatcgtg gtcaatcaaa ttatactgag tgaaggaaca caaaagcgag
    29761 ttgccaagat aactgagttt tctcaactaa atgattgatt tttcatatgg gggagtcaca
    29821 atgaaattct tcattaccgg agaaaggaat gtcggaaaga gttatcttgt ggaaagaata
    29881 aaaccggtcg ttaagtatag aggcttcgaa acacgttttg ataatgatct ggaaaattta
    29941 tacatgaatt tctacgaagg ggactcactt cttataggaa caagggaaag aggacgacta
    30001 agaattgtag aagaagggtt tttatttgcc acaaagcttc tgggctcgct tgacataccc
    30061 aacgacaatc tcttgataat ggacgaaatt ggctttcttg aagaagattc tccggagttt
    30121 cagcaagcgg ttgtctcttc tttaagacgt gcaaagcatt gtctctgtgt tctcagaaag
    30181 ggcaacttct cgtttattgg acaccttaag gcttcgatgg gtgtcgggag catcgaaatc
    30241 actcctgaaa acagagagga agtctatagc agaatcgtca gggagataga aaaagagagg
    30301 gcttatctac cctctcctgg aggcgacgaa gggatttgaa cccttgagta gagattttgc
    30361 agacctccgc ctttgacccc ttggctacgt cgcccaacga gaattattct agcatgtaaa
    30421 tgagatcatt tcaacaaaag ggccgctatt ggcggccctc ggggtggaaa attcttcagg
    30481 agggaggttg gctcccttag ttccttgggt ctggaaccaa ctcgcaacag gacgcgggga
    30541 catcctttgc aactcaatga tatatcgtta tctacccaga accaagttcg aatttatccg
    30601 acctgaaacg agcaaagtga tttagcactc tttatctaca ctatgaacac caatttgaaa
    30661 gtatgctcta ttctcttgca cccgtaatat ctggaaatta ccatgaaaga cagagttctt
    30721 tcgaatattc tctgctgaat aaacaggcat gaatcatttg tcaaaaacga gttccctgaa
    30781 ccaaatataa ctggtaagcc cgaaagtggg aattctgaaa actggatatg tgggaatcgg
    30841 accgtctccc gcctgaagga cataatcgtc aagatactcg atcctaccgt ccttagagat
    30901 caggaacatg ccgttcgaca acggtgtgct gtaaagctcc actgccgatt tgtcggcaat
    30961 caggtgtaac caggagtatt cttcaagaga gtctgcagaa gtgatttcgt cgccggagta
    31021 aagccataag agctcaattt ctggttttgc ggaatactcg ttccatgtga caagctcgtt
    31081 taaaaggctt ctcagcctct tctcttctct gctggtatct tcgcaatcac ctgtagcgca
    31141 tggaaatccg ccgttgaaaa cttcaagcgg tgtctttgaa gtcacgacta caatgatggg
    31201 atttccgaga taatcgctca tagagaaggt ctctccaaat acgtctctgg cttcgaacag
    31261 aggtgcgatc gaaccctttt tcaacagagg cctgttctgg gcatcttcat cgacctgctc
    31321 gaatgtgact tttctgccat ccctgctgat ctcgcttact ctgtagttct tgtcgttgat
    31381 tcttatgacg ctggtcgctt cgaaaagctc gtgattctta tgaaccatgt ctatagttcc
    31441 atctctgtct agatctattc cgatgaagac ttcgtctatc ttatcggcca ggccatcggg
    31501 cgcggtagga aacagggcga tttcgataag ctctccgtcg acttccagca aaccttctct
    31561 tctactcatg agcaagtagt cgacttcgac gccttccttt tttggaagga tcgcgtgaat
    31621 ttcaagatcc tgggtcatgt aagatgaatt cccgttggaa agatattcga cccttaactg
    31681 ttgactgaat ttataggcac ggctttcatt gataacggtc cctctatatt gtccctttat
    31741 cggaatgttg tttaggaaac agccgtcgta atcgctgtcg atccagagga tcggaggctc
    31801 gacgttcatg atcgctatgt tgacagttga atctccaatt gtgaactgtc catagaaaat
    31861 acgctcatct gcgattgcgc tgactccttc cggtggataa atactcattt cgacatcgct
    31921 tccgctcgcg atgtatgtcc agcccatccg ttccgtaaga taaccggaca gagtaccgga
    31981 aaaaagcatc gccgacgaaa tcaacgaaac gacaagcaat atcgctacct ttttagccat
    32041 acgaatcacc tcctcaattc atatgtcaaa gatcagagca aaaacaactt tcactcacag
    32101 cttcggcttc aaaatcatct tcgatttgaa agaaatagaa cattattgtg catttgttgc
    32161 agctggctac accaaatcct tcgcagtcaa catccacgta ctcccagctc cacggacttg
    32221 cgtcaatcga gtatctgcat tctccgcaag cgcttgcaaa tacactcaaa gggttgttgt
    32281 ttgcgtcgta atgacgtgct tctgtagaaa tacagcaata tactgcgtag ggtacaaatc
    32341 tcgtattaat tatcaactct ttgctgtaag aaaattctga atacaagaaa caatnnnnnn
    32401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctcgta ttaattatca actctttgct
    32521 gtaagaaaat tctgaataca agaaacaatt gcattcatac aaatagcaac ctgggtcctc
    32581 cactcttgtg atcacgccac cagtcagtcc aaatgtcaaa atcgacagga atacgaaaga
    32641 aaaaatcaaa aaacaccttt taaacatcag agttccctcc tttctagtta gttaaggtac
    32701 tgaaataata tcaccatata tgtcatatag aaaaatagta ataaacaaaa tcagatatgc
    32761 ttagcgctaa atattcgtga acagctatcc tgtgttacct aaatcgaaga ttgtcacagt
    32821 aatattgtaa tattactgtg attattaaga gaagtacacg atagaatgta gtgtacgtga
    32881 tgtgtgtacg cattgaatca tcaaaagaca tatgagacca tatgtagttt ttctccagaa
    32941 tttggtggta gctcccggtg atgataagtt cttccatatt ctgatttgct ccagactatt
    33001 tactacggat cacttcattt gccggtagtc atcacacggt attctatatg aacagcaata
    33061 tcatcacggc atatcgggct attgacagat agattcgagt aatgtagaat cttatctgtt
    33121 cggggacgtg gtgaagcttg gttatcatgc cggtctgtca caccggtggc cgcgagttca
    33181 agtctcgtcg tccccgccag agagagtcct gagggactct ctattttttt tgcatattgg
    33241 aagcttcaga caattctcac actcaagatg acaagcaagt tcttccggtc aatatctact
    33301 taacttcttt caggttgagg acggttacaa tatcttcaga gtgaatgtat tcgatacagt
    33361 gtccttgtga cttaaagatt aattcccgtc ggggcgtttt gaccattgtc gttgctgtca
    33421 agaatcgaag cctttcagac ttttgacttc tttgagctgg acttagggtg atgagtgttt
    33481 tgtaatagac tggtttctac cgagttctgg aggtgttcat agttgcctgc ctgtgttagg
    33541 gttctaggta tcgatccagg ttttggagcg gttggatatg gtgtagtcga gatgagatcg
    33601 tcgcggatcg atctcgtttc atacggagcg atcagaactg agccgcaaga acctatacct
    33661 gacagacttc taaagattca tgatcggctt gaagaacttc tactcgagtt tcgccccgat
    33721 gaatctgccg tcgaagagct tttcttcttc agaaatgtca cgacggcgat tcaggtgagc
    33781 gaggcgaggg gggttatact tcttgcaatg aggagacacg aagtaccgat atttgaatat
    33841 acaccccatc aagtaaaact tgccgttact ggctacggaa gagccgaaaa ggggcaggtt
    33901 cagaggactt tgaaaacttt cttccgaatg gaagttaacc ccagacccga tgatgcggcc
    33961 gatgcgctag ctgtagcctg gtgtcacttg acttcaagga aattccccgg aggtgttcga
    34021 aaggcatgaa actattcatg agaaatgtgg gactcccttt atcgaggctt ctttcgaagg
    34081 ctttgagcat cgactcatct ttcgagtgtg aaggaataga gatttccgat atatctgtgg
    34141 actgtaaatc taaagagatc gagttagctt tccaatgtga ggttagcaac gagataaagg
    34201 agtttcttga atctgtcttt tgtgagaagg cttctcgact tctagagtct tccgtgactc
    34261 ttttgtttaa gactttaacc agcgagggtg aaatacctga cgaagacgca atggaagtta
    34321 ccgagtctca gtttagagag atactccagc ttacgaatgg tgcatcttct tatcttagat
    34381 cctcggaaat ggagttcaaa gacaacaaaa taactatctc agttcacgac cagttctcat
    34441 ttcaacgaat cagttctaag aagcatgact tacgaaaggc aatagataag gtctgcaaga
    34501 gaagtattcc ttttgagata acactggaca tttcagagga tcaggactca ccagttgagg
    34561 aagtctcttt cgatagagaa gataatggag aaacggagca tttggaagaa agatcggatt
    34621 cagtcttgat gggtagatcg atcagggcag tctctgtgaa gatgagtgaa gttcttggca
    34681 atgaaaccaa tcttgttgtc agcgggagga tctttggact cgagtacagg aatggaaagc
    34741 tttcgattct aacgttcaac gtgactgact atacggattc cctcacagtc aaacttatcg
    34801 gtgagaatgc gcgctctctt tccgaaaagt tacgcgaagg cgatgaagta acgatcaggg
    34861 ggaagatgga aactgatagc tggatgaaag aagagattct gatcccgagg gatatcgtta
    34921 gaactaaact gccgaaacgc atggacaatg cgatggaaaa gcgcgtcgag ctgcaccttc
    34981 acaccaaaat gagttctctg gattctgtag tagatgtaaa cgatttggta aacactctta
    35041 aagattgggg ccacgatgcc gctgcagtga ctgatcacgg cgttgttcag tctataccgg
    35101 acttctactt tgcggcaaaa aagaaaggaa taaaacctat ctttggtatg gaaggttaca
    35161 tgataaagga ctcggaacag atatcaatga atctggaaaa agaggactcg cctctggaaa
    35221 gtgcgacctt cattgttttt gatcttgaaa ctacaggtct taatcctaag gaagacgaga
    35281 taattgaaat cggtgcaatc aagatgaaag gcaacgaaat tctcgagaca ttttcttctt
    35341 ttgtgaaacc tcagaaagaa ctctcgtctc taataacaag cctgactggg ataactgaag
    35401 atatgctttc aggcgcagga acaatcaagc aagttcttcc tgaattcacg aaatttgctg
    35461 aaggaggcat tctagttgca cataatgcct cttttgatta tggttttatc cgcaactcag
    35521 taaagaaata cttcggaggc gactgggaaa tgccgtgcat cgacaccctt gcccttgcaa
    35581 agagccttta cagagccaag agttactctc tggacaacgt agtgaagcga cttaaactgg
    35641 gacatttcga tcaccataga gcttatgagg atgcgaaggt tactgcagag gtccttaaga
    35701 aattccttca gcaagcaaag aagagaggag tcgagaaggt taacgagttg gaaaagctga
    35761 gaaagaacca gaatctcgac tcgctgaagc ctgttcacat aagtattctt tcaaagaaca
    35821 gaaagggtct tgaaaatctc tacaagttgg tcactgcatc acacaccaag tacttctaca
    35881 tgaagccaag gataccaaag agcttgctta ctgatttccg agaaggcctt ctcataggta
    35941 gtgcgtgtat ttcaggagag ctttccaagg catatatagg tggtgcgagt gtagatgagc
    36001 tcgaggaaat cgcaaagttc tatgattaca ttgaagtcat gccgctcgat aatattgaca
    36061 catcagaggg cgagactagt atgtctaaag agacgctgat aaagatgtat cgagagtttt
    36121 ataaagttgg gaaaaaacta aatatcccag ttgtgatgac cggtgacgta cattttgtca
    36181 atccacatga cgatatttat agagcagcaa taaacgcggc tcaggactat gacaactttc
    36241 agaatcaacc ttctctttat ctccacacta ccgaagagat gattggcaaa gctctggaaa
    36301 tctttgaaga cgaggggatc gctaaagaaa tcgttgtcga taattcgaga ttaatcgctt
    36361 cgcaaatcga aaacgttgtc ccgattgagg gtaagctgca cccacccgta atcgaaggcg
    36421 ccgatgaaga agtaaggaag atggccattg aaaatgcaga aaggctttat ggaaagcctt
    36481 tacctgatat tgtagaaaag aggctgaatc gggagcttga ggcgataatt ggtcacggct
    36541 atgccgttct gtatctcatc gcgcagaaaa tggtcaagaa atctaatgag gatggctatg
    36601 tggttggttc tagagggtcg gtggggagtt cgctcgtggc taatgttatc ggtattacag
    36661 aggttaaccc tcttccacca cattatctat gccccgagtg tggtaaatca gtattcccgg
    36721 aaaccgatct ggaatccggt tatgatctac ccgctatgaa gtgtcctgga tgtggaaatg
    36781 aaatgttgaa gaacggtcag agcataccat tcgagacctt tctcggattc gaaggagaca
    36841 aggttcccga tatcgatctt aacttttccg gggagtatca agagaaggcc cattcattca
    36901 ttgaagaatt gtttggcaga gaccatgttt tcagagccgg tacaatatcc acagttgctg
    36961 agagaacggc tttcggtttt gtcagggctt attcggagaa gagcggcaaa aatctacgga
    37021 gagcagagca ggagagaatt gcccgtggaa tagttggagt aaagaggacg accggacagc
    37081 atcccggtgg attgatgatc gttcctaaag acatggatgt tcataacttt acccctgtac
    37141 aacatcccgc aaacgataca aaaagctcga tactaaccac acatttcgca tatgaagtca
    37201 tccacgacga tttggtcaaa atagatgcac tgggacacga cgatcccacc ttcataaaaa
    37261 tgctgaagga catcacagga gtggatcccg agaaaattcc gatggatgat gaggaaaccc
    37321 ttggaatttt ttcctcactg aagcctcttg gcttgcgatt tcacgagctt ggaacggatg
    37381 tggggacact tgggatccct gaattcggta ctacctttgt tcgaagcatg ctttcagata
    37441 cgagaccctc tacttttggc gagcttgtaa ggatatcggg cctgtctcat gggactgatg
    37501 tttggcttaa caacgcgaag gatatcatca cttcgggaca tgcttcattg tcgcaggtta
    37561 ttgcctgtag ggatgacatc atgaattatc taattggcaa gggaatggag ccaaaagagg
    37621 ccttcaagat tatggaaaag gtcagaaagg gtaagggcct cagcaaggaa gatgaaatag
    37681 agataagatc ttgcggtgct cctgaatggt tcatgaattc gtgtaagaag atcaagtacc
    37741 tctttccccg ggcgcatgcc gccgcttacg ttagcatggg ttacagagtc gcttacttca
    37801 aggtgcactt tcctcttgcc ttctacagca cctacttttc gatcaaaggc ggggaatttg
    37861 atatagacat ctgtacttcc gacataaatt caatcaagaa agagatagtt cgattaagat
    37921 ccgatcaaga tagaaatgtc aaggacagag cgaaggagac acttcttgaa gttgttatgg
    37981 aaatgaaact gagaggattt tcttttctca atgtggatct tatggagtcc caccctacga
    38041 ggttcttgat tgaggggaat tcactaagaa ttccctttaa taggcttcaa aacatgggcg
    38101 acaaagcggc aagatcagtt gaagttgaga gagagaaaag gccgttttca tcggttgagg
    38161 atttgatccg cagaactgct cttaacaaga caagtgttga gatgttaaga aaatacggag
    38221 ctttgaagaa tctggccgag aaagaacaga tcagactgtt ctgaggacga aaatggattt
    38281 caaaataagc gcttcagatt atgcaaagct gatcgatctt tttggaagag tatccggtac
    38341 tctggaaccc tcctacagat actttcagat ttactacaag agaggactga agttttttgc
    38401 ttctgatggt tgcatgaagc ttgagttttc ctccggcgtt gctacctcac ctttcaatgg
    38461 agtttatacg gtaccgatcg attacgctcg agctttgaag agttctgacc caacctcatt
    38521 aattttcttt tctgcgggtg aagaagacct agagctgagg acggcctcag agacgcttac
    38581 tttacaaaag gcccgcagca gcactcttcc ggctatggaa agaaagtttg agttcttctc
    38641 gcgctttccc ctgaaggaat tcagtagttc tttgaacttt gtttcatctg cttctgttga
    38701 gggagaaact gtcgagatct tctcaaagaa gaacgagact gttcttgccg catcggcgaa
    38761 cagaatggta ttggtttcag ttcttgaata cgagccaaaa accgagttcc gttactctct
    38821 tcaatatgta accgttaggc atctggtgaa ggccttagag ttgctcacat cggaagaact
    38881 ctatgctgga acagggattt ccgagctggg gctgaagatt ggtcctctat tgttttcaat
    38941 atgtactgat gaagcggatt tttccgaacc accatccttc tttttcggag acatcgatga
    39001 tggaaatgac gtacagagaa aggcctttct ttccgctctc agaaagataa cacggttatc
    39061 aaagcatgga ttttcggttc tgatgatttc tgacaagagc aagttgaagt tctttctgaa
    39121 ggccggtgct ctccgttatg aatgcgaggt gtgtgatttt gttggtgatg aattcgttgc
    39181 gcaggtagat ccgagaaagc tgcagtctgt tatttcaagg ataaaagatt ctaagattcg
    39241 tctggtaaca cgaaagggaa atctgcttat ggtgggaagg aagagcaaga tgtaccttat
    39301 ggtgcccctt attaggtgat atgttatatc cgcatctgtt agtattggta tgactggatt
    39361 cactaatgtt cagcgaaatt caagaaggta cttcaaaagt cggagttgaa aaatatgggg
    39421 attcttgaaa cgaaaggcct tggaaaaaac tttggtgaaa acaacgtctt taggaacgct
    39481 gacatagaac ttgagagtgg gctgcttaca gttcttggag ggattaacgg ttccggcaaa
    39541 tctattcttg taaagacaat cagcggtgac attctgcccg attctggaaa ggtaatattt
    39601 gaaggctctc cagtggattt gacatcgatc gtcgaggcaa gacgtctagg cataagtgcc
    39661 atctatcagg agtacatgtt agttccggat ctcacggtcg ctgaaaacat cttacttggg
    39721 agtgacaaat tcacgattag cagaaggaag atgaacgatc aagtctcgcg ttttcttgag
    39781 cagcttggcc tggagttcga tccttcctcg aaagtctctt ctttttcatc tgatgatcag
    39841 gttcttctcg agtttgcagt tgcagcgttt tccaggccga aggttctcat tttggatgac
    39901 ctcttttctt ttgtacgtgg tgcaacccgg gtgaaactcc ttgaaatgat agactcattt
    39961 aaaaaaggag gagtggccgt tctagttaca acgtccgatc cagaagtgat ggtgctgggc
    40021 gatcgcctat tctttatgga ttattctggc atcaaacctc ttccactgca aactaaggaa
    40081 agtgatatat ggaagatgct gggtgtctca cctgagtact cagtagcacc agttggaggt
    40141 gaaccgttaa tcgacataga taacctggca gataccggac tcgatctgag gttgaaaagg
    40201 ggagaggttc tctgtatttc ctgtgacaac ggagtttcgg ttagagaatt cacaagagcg
    40261 cttacaaggc aggacccttt ctccgagaac tctgtggcaa tgcttcgaga ggcgaagaac
    40321 ttctttgaaa gattctcctc cagaggtcat tctagcgagg ccgaaaaata cttctcctct
    40381 aatgtgaaga acaccggtct tgtttcgcaa atcatgttga aaaagtcccg taaattcacc
    40441 tcaaatagat cactgaaaaa gagtttcatt ttaaacaaag aaagcttggt ttccgtgaag
    40501 agttttcttg ctgggctggg aagagctccc ggtttctccg gtctaatgcg cgatgacgat
    40561 acaaaacttt cgcatttgag attcgatgct tgcgatctct ttatatttat tagaccttta
    40621 ataggactgg acccagtttc gacgagagcc ctcgcagagg ttctgcagga gattgctgac
    40681 cgttccaaag ccgccgtggt aatttccgac gaaatcatga gccgcaagct ttgcacaagg
    40741 ataatgaaca aatgcaattg aaccctgatt tctcttacga gaagaagaag attgagtttt
    40801 ccagcgggta catattcaaa gggaaacact atagatgttc gattatcaag tatccatctt
    40861 tctacaggaa caagatcagg ggaacagaaa acgttgaaat ctacaatttc tcaccaagag
    40921 aaagagctgt aggctcgata atcattcttc atggactggg aacggtaaat atcccatttt
    40981 tgttctggat gggaagccat cttgcaagtg ccggtttgca ggcttctgtg atgatccttc
    41041 cgggaaatta cacacgaacg gcaaccggtt caactagtgg aaaagatttc ttttctcctg
    41101 atctcagaag gttgattgtt ttctgggagc aggcagttgt ggatacaatg acaaccatcg
    41161 atctccttca acaagagaaa ctctggtggg ataacaactg cctttttggt tactgcctag
    41221 gcggaatgat ctctacgatc gtgaacgcta tcgatcaaag aataaacgac atgatattga
    41281 tgaccgttgg aggtaacatt gcgaaaataa tatggcagtc accggtattg aaatcaattc
    41341 gtgattcact taggagcggc tcgggaaagg agggctatct aaacgacgaa gtgaagttaa
    41401 atgctagatt cgaagaggat gctcgtgcag tgaaacttat aaaaagtgca catgaactga
    41461 tcgagtccga tcttcatcct ttgtttaaga tagatcctca agcctatgcc cgtttcaatg
    41521 acagaaagaa agtaacgatg attgaggctc tcttcgacaa aactcttccc atagaaacta
    41581 gaaaacagct ttgggaagcc ttcggaagac caaagaggta tattgtgcct gtaggacatg
    41641 tatcctggtt tccctttgag tatgctcttg gtaagttcat actgaaaaaa ctgggaataa
    41701 aggaggccag aagaaacttg aggctcttgg aaagtactaa gccaactatg gagtagtcgc
    41761 caggcaaatg gaaaacaatt tctcttctta ctcaactttt cctggccaaa gccgatactt
    41821 ttaattaact actcttgcca agcagcgagc gattaaactg tattttcctt gaactcttca
    41881 actaattcaa gagtactcaa accttctaac tggcagtagc agatgaccta tctgatgctt
    41941 ctttttcact ccagtcggat tacctacaca ctcacttttt cgccttgaag aggacaataa
    42001 atcagtcttg tttctcagta tgacagattc ctttcgaaca gctaatcgaa atctgtttct
    42061 ataggcccaa gaaacttaca aaagaatcta caagattggg atcgaactgc tttcctgcgc
    42121 acgttcttag ttcttcaatg gcctcttcag aggaaacggg atgcttatat ggtcttccgc
    42181 tcatcataac atcgtaagca tcaatgatgg agattattct ggcgagtagt ggaattgatt
    42241 cacccttaag acccctggga taacctgaac cgtcccacca ctcatgatga ctaagaatct
    42301 catccgctat cccgataagt tcaggtgagg aaagagctat cctaaatcca gcctccggat
    42361 gagatttaat cttcttccat tcctcggccg ttaaacttcc ggctttggta aggatctctt
    42421 cgggaactgc aatcttccca atatcatgga gggcgccgag gagcaagagg ttgtcaagtt
    42481 cagcttcatt caaaccgata gactttccga agtctctgct gattgtctta attctctcag
    42541 tgcgttcttc cgtttcatag ctcttgttga gaagactcct ctcaaggacc tcaactgtct
    42601 tgttcctcat cgatatgttg ttcaggagtt tatccctgta cattttttct tctgccgaca
    42661 caagggtttc gctgaagtcc tggtcgatgt cggacttggt tcctatgcca aaggagacac
    42721 tgattggaag actgaaacca gttacctgat ccagagcctt tttaatcctg ttaaccagag
    42781 actctgctga ctgagcatcg gtttgaggaa ggagcattat gaattcatct cctccccacc
    42841 gagcgatgat gtcgtcggat cttactgaag atttaaggca gtcggcaacc gctttcaaaa
    42901 gtcggtcgcc ctcgagatgc ccgaaggcat cgttgacgat tttcagccca tttacatcac
    42961 ccatcacaat acttatcggc atctgacgct tcacgtttag cctgtctatc tcttcctcga
    43021 agaatgatct gttgtacagc gcggtaagct ggtcgtgaaa tgtcacataa tggatttccc
    43081 tgtcggatct gattctccaa atcgcttcaa ccgcatgtgc catcaaggtc tcaaccatct
    43141 tgatttcttc ctgtttcatt ggagaatcat ggtaagacac tagggcacca atgcttttca
    43201 tcggaacaat tgaaaccgag tctttacagt tgggaacaac ttttctaata gatgagcttt
    43261 caataaaggc ggatttgcca gtttcaacgc agtctcttag taccccgctc ctgaaaaggt
    43321 atccctgaac ggacgccacc tctccctggc tttcgagtgt cactaactcc tgatcatata
    43381 ggagatatag gctgaacttc tccatgccca atatatttac cgatgcatcg attattgagt
    43441 gatatatagt gtcctcttca tttgacttct ccattttgag ggcaacaaaa tggagtttcg
    43501 acactttgtc ctttgttctt tcaagttctt cctttgccag tttctcatcc gtaatgtctc
    43561 taactgctcc acagatccct ttaattttcg atcctccgcc acagtacttc ggctttgaaa
    43621 actcgctaac ccatctcaga gctccgtctc ttctaataat tcggtactct attctttctt
    43681 ctttgcccga cctcactttt tccaaatgct gtttgtaaca atccaggtct tcgggatgaa
    43741 ttatagtctc ccagttgaat tctctaggtt ccggggaatc aaggctgtat ccgctaatcc
    43801 tttcatatga ttcacttctc cagatctcag gatcctttcc atcgctcggg aactcagtct
    43861 cgtagtagta atcggaaatc gtctccgaca tcgatctgta tctttccatt gaaagtgata
    43921 gctgggtttt tacagatctt tcctcttcaa gcttcgcact cagagaagcg gtcttctcct
    43981 ttacctgtct tctcagagtt ctgttccaga agtacattaa aatcagcacc aggaaaacaa
    44041 cgcttgccac gggaattgca aagaagagcc aatagagcat atcaccgcta ctgtcttcag
    44101 cattaatatt gccgaaccac ttgttgaaga tttgcctgta ctcgcccgtc gaagagatta
    44161 ttgagagacc ttcattcaac aaagatagca gctcggagtc accatgtttc acggcaaaac
    44221 agtattcaag acgagcaaca ggatgttcag aaatcataac gttcttcagc gagagctcgt
    44281 caataagaaa taatccctga atagtagaca taagtaccgc atcgacctcg ccgttctcaa
    44341 gcattcgcat tgcaatctca gggaattcga ccctcacaac ttcgctaccc actccctcgt
    44401 tggttacaag ttcatccatc agatcgccct tttgcacggc caccctggac tctcttagat
    44461 catttaagga ggaaaacgcc tcggttcgat tactacaaaa gatagaacca tggaggattg
    44521 tgtgcggatt ggagaaatca acgagaagac ttctgccttc tgaatagtac attcccaaca
    44581 aaccatcaat ttgaccggac tcaagtgcag atctagcact tatccatgtt ctgagctcta
    44641 tttcaatctc gaaatcgagt gcccttgata ttgcctgaag aatatcaacg ttgaagccaa
    44701 caggtattcc tctttcgttc tcatattcga aaggcgggta atcgatatcg ccactgaata
    44761 cgtatttcat ctgtccaaac aaagtcagag ataatagcat gaagaaaaca attgaaaatg
    44821 cttttctcat aacacactcc cagaagaagg ctatcctcct cagaatttac tataacatta
    44881 tgcggcctaa atctatgtgg agagaagaga gtatttgtcg acaatcgttc gatcacattg
    44941 agatgcctgt tttggcgcca ccgatgtgta ataattgaaa agaggtgatt gcattgagaa
    45001 aaactctagg aagtgtagat ataggtttga ttattggatt ggtttcactt gtattggttg
    45061 gtagccttgc atacctcttt cctaccgcta agattcccga tccaggtggt aagtacgaag
    45121 tcggagtcga gattattgag ctggatgata acagcaggat cgatccattt tccgacggag
    45181 aaatgatccg caggcttgtg attgatatat ggtatccctc ggatgataca tcgggtaaga
    45241 gaagaagccc atggttcagg agccattcgc tttttgtgaa cagcctggcc gataattata
    45301 atcttccacc catgctgttt cagcatttga gagccgtcaa aactaattca tttattggcg
    45361 cggctccttc ggccaatgca aataatctac cggtagtaat cttatctcca gggatgccgg
    45421 ctatagttcc cttatatttt tcatttgcgg agtatttggc tagcagaggt tttgtcgtgg
    45481 taggtattga gcatccgtat gccgcatcag tcgtggaatt ttccgacggc agtcagtttc
    45541 acttcaacaa agatagagtg ctcgatcttc ctggagttga gacgtttgaa gaaggtgcta
    45601 ggctttcaat ggagttgatg gccgatgatc tgtcttttgt aatagacagt ttgtctaaat
    45661 tgaacgaaag tggacatcag ctggcgggta tattcgatgt tgacagagtt gctgcatttg
    45721 gtcactctgg aggtggcgga gttgttcact tcgcatcaaa tgatgacagt cgaataaagg
    45781 ctttgctttc atacgatcca gcgcttttca tgctcaatga taatgagatt gaagagggta
    45841 ttggaatacc ttcacttatt attgaaaccg atgagtggaa attccgagaa gaatcgggaa
    45901 ggatatcgga tctgattgaa agcagcgttt ttcctccctt acatataaag atctccgaag
    45961 ccagacaccc ggattttgca atgctagatc atctgtcgcc gttagctcat tttcttgact
    46021 tcactggtag ttttatgaag agcggtggcg aatattatct ctacgacatt attcaaagct
    46081 tcctcgaata tacatttggc aatttgagtg aaagtgaatt actgtcgaga cttcttcaaa
    46141 gggatgatgt gagagtattc aaggggatcc atgagatccc taggtaatgt tttcctcttt
    46201 tagcatcaaa ggtacgactg aaatagtgat tgctgtattc actatcgtgc ccgttgagag
    46261 cattctgctt agaaagagag aagtctcagc ggcacgttat ttgagagcca aattccgtcg
    46321 aagagtcaac tgtgttcaaa agaattgcgg gacataattt tgcgtttatt tcaaaaaact
    46381 tatattgtac tggatcattt gcgtagttaa ttgaggagtc aagcattggg caaggagagc
    46441 ttcgggcaat gtcaggaggt agttcctctt ctattatgtt ccgtttagct ttgatacacg
    46501 atctcggggt cgatgacaac cgtcactttt cttagattca ctccctgaca ggaggttaca
    46561 gatactgcta ggagaagtct cgggaaagct ggcaaaaggc ctggattttc gacttctctc
    46621 gaagtcttta ccagagggcc ttttctttcg tataagctga acagattgag cattatctaa
    46681 attgaaagaa cggaatgata tgagagatct tctgtgattc tttggacata cagtctatat
    46741 ttccaatgaa agaccatcat atgcgacagc aattccgaag cggacgtaga accgttcgag
    46801 ttcaccctgc gttggattag gatagtggga gaagtgatgc gcgtaaatct tggttgtgtc
    46861 gtccattatt ccgttttttc tcattgtttc cactgtcgag accacctgtt ctgcagtttg
    46921 atgatatggg aatgtgacct ctttcatgaa tcccagagtt gagtctatcg ctaccagatc
    46981 caaaggagag tctaagaaac tctttatcaa ttttagtgaa aactcatcta aaggacctgt
    47041 atcagtggca tagaagaagg atttaccctt cttcttaatc aaaaataaca gagcctgttc
    47101 accttccttc actttgtgga cagctggaat aggtatcacg ctataatcgt taagcacagt
    47161 ttctttgtaa gcttctgcca ttaacaaaat agttttgtcg ggatcaaagt acactgaatc
    47221 cgaaatcaac gacagaacag tcgaattcgc tagcacactc atcggagaaa gtctttcata
    47281 cgaagcattg tatctgtcca ttctgtaacc aaagttaggc agatatagat ggtctgaatg
    47341 agaatgagtt attagaaggg ttctgacatt gaaaagtgtg ctcccggtct tctgagcgcc
    47401 ggccataatg tttggcccaa aatcaatcaa caagtcatca ttaacaagaa cggccgaggt
    47461 caaacggaag ttgtctggac cggcctctct gacctttcta caattgtcgc aatcacagaa
    47521 cacgttggga tgtccctcat aagctgcagt tccaagaaat aggactctca tttttcccct
    47581 ccaaatggat gattgttgaa aattatcatt gtggataagt aacaaaatgc caatacagat
    47641 tgggaataga aaacctcatt cccggcaaaa aaagcttgca atataaacac tcttatggaa
    47701 tcatctggtt tttcttcgaa tcaaaggaga ttctttccat aagattggtc aaagaagacc
    47761 cgccaaagga tcccagtatt ttggctactc catgtccagc atggcccttc gaaatcgatc
    47821 ggctcaaaga ataggttgag ccttacttca ttacgcgatg gatttcctat tggtcgttgt
    47881 attcacagat tctctgcctt tcaaatcgta tctatataac cagcactgac caaccgtcgg
    47941 cagctgaaca aataatcaga gcgaagcgtc tctaagagac tcctcgattc gtctatgcat
    48001 agtgactaac cgatttcatg gtaactgggg ttttcaaaga atcaagttca tactttagga
    48061 gattcatgca cttctttcta tgtggttgaa accaaaactc attttgatga aactttctgt
    48121 ccctccggcc atataccatg acatttcata agcagatttc gcttgacatc tcgcaaagca
    48181 aagtgcatct cagatcttca gcgggacacg atcctgttct ttcctgtgtt ctttgcttca
    48241 tacatcagag tatccacttc aagcaagaga tcattaaccg attcatcact ccattcggca
    48301 actccaatgc taatagtcac aggtatttcg ttagttctct tgagtgactc acttattctc
    48361 tgcgcagtgg aaaaagctcc ctcaactcct gttgaaggaa gcaaaataac gaattcctct
    48421 cccccatacc gaaaggcgta atcaatcttc ctgatactgg atataataag cgaggaaaga
    48481 gattcgagaa ttccatcacc cttctgatga ccgaaagtat cattgatgac tttaaaatca
    48541 tcaatatcca gcatgattat ggatagcctg ttgccatatc tcttggctct attgatctcg
    48601 tactcaacta tttcaaacat cttcttttga ttcatcagcc cagtcagcga atcggtttca
    48661 gccctttcct tcaacaactg atttgcagat gaaagctgac tttgcatttc taatagttct
    48721 tcgttctttt ggactatcaa tctgttcgct tcatcgagtt gtcgatttct ccttgccata
    48781 ctttcgattt ctttgagttt ttgcatcgtg gagaaggatg attgcaactt tgcgaccatt
    48841 ctcgcttcct cttctttgaa aatctctgcg ttcagcttag atatttctct atgggtttcc
    48901 agagctcctt taacgtcacc tacaatctcc tgtatctccg aaatgactgt aagtctctga
    48961 agaatctccg atttgttgcc ttttgatttg tgatattcga gagaagtgtt cagggactct
    49021 agggccgaac ctaattcacc aacagctctg taatagttcg cctcataacg gcagacttct
    49081 gcaagagaaa actcatcttc aatctcgttg gcaacctctc tagctctttt cagcagagaa
    49141 tgtgcttcat cctctttgcc cgtatatgtg taaatatcag caagattcat gtagctgttg
    49201 ggtaggtcgc ttttgagtga gttctcttct ttgagctcaa tggccttctc gataaatacc
    49261 gctcctgaac catcaccttt aatattctca actgcgattc ccttgttgtt gagatatttg
    49321 gcgagagagt gattgtcatt atttctctga gagagatcga tagctttatc aatatattcg
    49381 agagaagtct cgaattctcc cattttgagg tatacgccgg caatgttgtt atagatcata
    49441 ggcaatcgtg aatcatcccc aagctcatca aagacagata tcgcgctggc agcatactcc
    49501 agagccttcg aaaactcgcc tgattgacag ctaattctta tcattgaatt atagacaaag
    49561 ccaagttcag aactgcattg atttccgatt ttttccaact ctctgccaac aatttcaaaa
    49621 gcaatctggg tttctccaga atcaacgagg gatctctgaa tccttttgag aaacgaaatt
    49681 ctcgaatcaa actccgattc atctttggac agatattctt catacaagtc tgcaaaactc
    49741 ttttcctgtt ttatatgatc catctcatcc acccttttca agaagtagcc tggcttgttt
    49801 ccagtctgtc ctttcccttt gttttggctt catagagtag cttatccaca acggcaatgt
    49861 atttgtctgc cgactcgcca ttccactctc tagcacctgc actgaatgaa accgttatct
    49921 ccagttcttc ccgaaaggat tccttcaacc ttgaatagac ttcggttgcc ttgtctagat
    49981 cagctaacgg cagcaaaacg aggaactctt ctcctccata cctgaaggca aaatcatttt
    50041 ttctaataga agacttgatg attcttccaa tttccgaaag aacctcatca ccctttagat
    50101 gaccatatga gtcgttcacg aatttgaagt tgtccagatc gatcattatc atcgtaagag
    50161 gagtgccgta tctctctgct cttgcgattt cgttttcgac tatagtccgc atctttctat
    50221 ggttaagcaa accggttagc gaatcggttt cggcctgcct ttcgagaagc ttattggttt
    50281 tctcaagcct gcgtttcatg tcatgcagtt cgttgttctt ctctgatatt tcttcattta
    50341 ctcttctgag ttcactgttc tgctcaagaa gagagtgcat ttctctgagg ttttctcttg
    50401 cttcgaaaga tgactcgagt tgtgctaatg aacgcgcttg ttcttctttg aatattgtac
    50461 gattcagttc cgttatcagt ttgaatgttt gcaaagcttc attcgcatta cccattaact
    50521 catgtattga agagatctca ttcagtacgt ctagcagctc cgtattgttt ccgttttttt
    50581 ggtgatactc gcttactcct gtcaacagct ccagtgctga gaagagctca ccttcggcct
    50641 tcaaaaagcg tgccctgtac ttcattgtct ctgtcagagc tcgctcatcc ccgttttccc
    50701 ttgccatagt agttgctctt tggagtaatt cagaggcctc tttcttcttt cccgaatcgt
    50761 aatagagctc tgccagattc aaatagctgt tgggcaattc atccttcatg ttgttcttct
    50821 ctttgattaa aatcgcttct tcaagatacg gaatgccaga tttatcagtt tccgagttct
    50881 caagaatcac cgcaatattg ttcaaacaac aggctgtatt agtctcgtca ccattttttc
    50941 tggccatttg cagcgctttg ttcgcattcg aaaaagcctt atcatactct ttcattttga
    51001 agtagatccc tgaggtgtta atcaagactc tggttagcaa tccctcttcc tctttttctc
    51061 tgcagattgt ctgagccctt ctggaatact cgactacgcc cgcgaaatct cctacaagcg
    51121 cactcagttt aatcatggag ttcaaaagca ctgccatttc caaagaatca caattatcga
    51181 atcttcttat ctccctttca acgattccgt gggcgacaag tgtatttccc tggtgattaa
    51241 ggctggagat agctttattc agataagtta tctttaggga aagatcgctt tctggcaccg
    51301 acatgtattc cgtcagcaac tcaacaactc tatctttgat ttccgtattg tcttccatca
    51361 ctgtctccat ttcttgtatt tctctggatt taagtgcttc taattattca cgagatagag
    51421 ctaaagcgcc tccctgaata tggtaagtgg aagcttaatc aaggaattaa gtccgcatct
    51481 ttgtgatcat catcattcca atataccgtg atcacttcga aattatcaag cctcttttcc
    51541 agtataaccg tcatttcatc gtccgacact ttcagtctgt tgtagtttgc tatctgtacc
    51601 cttatgcctt cttcaaggac tctaattacc ctatccgttt cgattcctct ttcatccatt
    51661 ctttgaacgg catgctgact gaaagagatt tcccgttcct cttctctctt cgctacttca
    51721 tctttctcgc ttaaaccttt tatgagaaat gcaacaacta caaccgcaat aatcaccgct
    51781 aaggtgaaga acatatctgc ctccaagatg ttctgctctg tggtttttag atctagtcgc
    51841 tttccccaat ctttagaagt atgtcatcca ttatatacgg atcctcctta ctaccgcctc
    51901 cgaaatggta gctcttaggt tccggataga tgtcccggta aacggcttcg ataccatcga
    51961 aatcccaggt tgttatgtat actttgaatc catttaggct ttctggtctc ccgatggttt
    52021 caccgggaat ccttagtact acctcgctct ccattttgtt tgtctgtaca agaggagttg
    52081 gagatattgc tgtgccgaat gacttggggc cgcttccttc tgcagaatac gcaacaatcg
    52141 accaaccgtt tgcaaaaatg aaataatccc agtcaagtcc ggctggcata gttgcattca
    52201 agaaaggtag gaccgttgaa ccagtcttgt ggggatcatc gatgaatatc tggaaagtga
    52261 catgatcgaa tccgttttgt ggtccccacg tgtcagtcaa gtccttgatt ctcatgttaa
    52321 gagaaagcga tgctcctatc tgtctcaatt caactcccaa caggtccatc tgattcttga
    52381 aagagatatc cgttggatac tcgtatcttc cctccggtcc tttgtcgtct ccttctggat
    52441 cggaaagcga agacaagagc aattccggta tgtcgaggac aactgtatag tcttcactgt
    52501 aaattgactc ccttttcgtt cttccgtaaa ctttaaagag aatcgagtga attccaggat
    52561 cgaacttcga tatgtcccac tcgtaagacc actgtccatc ttcgatttct atatcttcgg
    52621 cctcctcaat ccttgcatca aagattatcc ttgcagaaga agctcctgag gcagttcctg
    52681 tgatcgtata tgtcgaagca atcttctgag catattctag attggcaatg aactggattt
    52741 cgggggattc aaccgactga tattcctctg aagccacgcc gacatatact gagcggggat
    52801 tcattgtcat taggagtttt ccgttctcat ctaccggata acttttcgaa agtgagttga
    52861 aggtgtaaat tggcttgatt acctgaccct cagccaagcc tgactccatg tttgctagaa
    52921 tccgccgctc accagcggta ttcattatga cgtagatttt ttcgttgtca tgctccaggc
    52981 tgtaagcaaa gatccccggc ccgttgggat ctgatttcag gacttcaact cttccgtatc
    53041 ttaatgcagg atgctcaagc ctgagtttgg ccaaatcgct cgtgtactta tataactcag
    53101 attgcgtttc aaagtggttc agtccgccag attggaaacc gtcttcaaac attgccgctc
    53161 ttgtttcaac gaagccctgt tccgtaccgt aatatacaac tggtatccca ggaacagtga
    53221 agatgaaggc cagcgcctgc ttgaggcttg ctaatcccgc tcccttaaga aacctctcca
    53281 tatcatgatt atctatgaag gttaagagtc tagtatggtc gaaataactc tgccttgtct
    53341 cgagtctgta ccctaaattg gcagtaggtt ttccctcctt gagaacactt cggagctcaa
    53401 tattcagagg aaaatcgagc attgcattca tgccagattc gaagaactcc ccaataactc
    53461 tttctccagt gtcttcaaat gggtcgcttc ttacccaggc ttcaccaaat gtcaggaagt
    53521 tcaatttgcc gttcttgctg gcgatttcgt aaacacctga ctctccattc aagaattccc
    53581 tccagaattc gtggggcacg tagatcacgg tgtctattct gaatccatcg atgtcagcct
    53641 catctatcca gaaagtataa gaatccttca acgcagagat tactgaaggg ttttccgtgt
    53701 tcaggtcatc gagtcccgac atctgatagt tgaacttctg atcctggtcg ttgaaattag
    53761 atatgtcagg tgtccagtga taaatgttct cgtctctgtg ctccacaaaa ttgttcagag
    53821 agaaggggta ttgctccgga gaatccgatg gaacgcttcc cgaattcatt tcgaattcgc
    53881 cgtccacgaa tctaaaatag tcacccacgt gattaggaac tatatcctgt attacaagca
    53941 gacctttctc atgggctttt tctaccagct ccttatatag agctatgtcg ccgaaatgtt
    54001 catcgatcct tttgaaatct ctcgcccagt aaccatggta gccaccgtaa ttgacccaag
    54061 gattccacca ctggttggca acgggaggcg taatccagat tgcagtcgca ccgagtcctt
    54121 tgatataatc tagcttatcg attattccct tcaagtcccc accattgtat cttgagttat
    54181 cactaccagc ctctcctaat cccatgtcgt cgttggatgg gtcaccattt gaaaaccggt
    54241 caatcattaa aaaatagact acttgatcct cccagacagc tgacgaaagg cagattggtg
    54301 ttacgaaaag cattaataga agagcgatga atactttctt catacactta acctcctgag
    54361 tattagatca aaacgctatc gagaaattcg ccaaggctct tgacccggag tctgtggtct
    54421 atatgtctgt tgtatggcca gtccattatg tatgtattga tcccgtgatc aagtaggttc
    54481 tctatgtgat ggggggcgtc gtcgaccgcg aattcaatgc caagtacttc gcatagttcg
    54541 ccctttcttg aactgactat taccgaaact ggttgaccga tctctttctg aatccatctt
    54601 tgagattgga tctgaacatt tctccctttg gtgggaaagc gagaagtaat gaaatagacc
    54661 tcatgctgac cttcggctat tagttctcct aatctcctaa gatcctcatc gtttgcgtat
    54721 gaaggtagtg attcgtaaaa gtttggtgtg tttctaatcc tttcccagac cctttcttcc
    54781 atttcaagcg tcaagttggg cacgcttttc cagaaatccc agatcttgac ttcgacttct
    54841 atcggtactt catcatacat tttatgggca attctaagaa atgcaagatt gaagtttgtg
    54901 agaatatcat ctatgtctat cataattttc atcgcttcac ctccgcatag aaaaagtata
    54961 tatcatccaa gtgtctgttc tagtaatcac caaagtactc ttgcaactct aatcgtcaat
    55021 acctgccgtt aatttgtttc cgttcattct ggattcacca agctttgtca ccaaatgacg
    55081 tttgtgggat aatcaagaca aggtcttctc tcaaggagga agaagaatgt tcgaactcaa
    55141 ggaagtcgca ctgatatatg aaacaaagaa acaagctctg gcagcgctaa aatcaacaaa
    55201 cctaattata gaacaaggag aagaggtagg agttttcggc ccttccggtt caggaaagtc
    55261 ttcccttctc ttcatcatga gtactctgaa agagccaagt cgcggcgaag ttaaatatga
    55321 aggtaaggaa attaccgctc ttactccatc ggcgaaggcg gaactaagac ggaaggaatt
    55381 tggttttgtc tttcaggaac acttcttgat taatcattta actgtgagag aaaacatcct
    55441 ggttcctata agagataaac aaggaatcaa aaggagagtc gaggagatta ctactgcgct
    55501 tggacttgaa gacctgctga agaggtttcc ttatgaactc tcagtggggc agagccaaag
    55561 ggtagctgta gcgagagctt tgataggaaa tcctaaggtc gtttttgccg atgaaccgac
    55621 cgcttcactc gatgatgaaa acggtcgaaa agtaatcaac ctgttgagat cttactgctc
    55681 gaaatcagga gcgactctaa tcctggtctc tcactcagcg gaaattatcg ctgattttaa
    55741 gagaaggatc aaagttgaaa acgggcaggt atacgaggat actgtgaatg cttaagtaca
    55801 tttggcttta ttttaggaga aggcccggga gaattctagt cttcataatt gttgttgctt
    55861 taggagtcgc tgtaagtatc gttttgagct cgatatttct atcttttgga gagaccagaa
    55921 gcaggatctc gaagatcaac gaaaattgga ttactgtgca gtacctttcc gataccggta
    55981 gaaaagaaga gatcgatgac aacctcaaag agatttttgc agatgtatct ggcatttcag
    56041 atgtgatcca ggtagatatc gcttatcttg gttacaatct ttttggaagc gcgagagcga
    56101 attttcctgt ttacggaata aagcaaaccg acattgcacg gattttgcag gtgagccagt
    56161 cttacatagt cgacggaagg attttctccc ctaaaacaaa tgaaataatg gtttccgact
    56221 cgttcttaaa ggcctccgga gtcggcatag gactagtata cgaggagatc ttcgagacgg
    56281 cgagcagggg gctgtataat gttgttgctg cacttaaagg cccttcggtt tttggaatcg
    56341 gtccgtccgg gatttcagga ggaaaaggcg cttatggatt catagtgctt tcggaaagag
    56401 gtttttcaat agcggtcgaa agtgagttga ggtcaagaat agccggtgaa gaactcaaag
    56461 taggggttca aggtcccatc tcttcaagag agaatatcga aagaagttat tccggttatt
    56521 acctgggagt cctcctgtgc aatctctgcg ttgctcttgt gtttataatt ggacttacaa
    56581 tgcttaactc ggtcagcatt cgtgaaagaa gaaaggaata cggtattctg gcggcaattg
    56641 gccattcacc agcgagcctc ggtgcgagac ttttctttga atccgtatac cagtgtgccg
    56701 ccggttggat tattggtctc tttgcgggaa ggattcttgt ttcatatcta gagactcgat
    56761 ttttcgaacc caacggtctg tttgtcggtg atatctttgt ctctacgata tggacgctag
    56821 ttattccagc agtaactgtt gtattcactc aagttttgat cgggagtcac ctgagccggg
    56881 atcttgtggg acttctaaag agctcggaaa agaagaaagg gatactaaag aatagattta
    56941 agaacataag tttgccgtgg cttcgatttc cgctgagaag cgacggctac agatccctat
    57001 ttgtaaacat aatagttttt gcccttctcg ttacgctctt cggatcattg ctttcctcgc
    57061 ttaccgatac tatccgcgat agtggtcggt tcttcgatag ttgttcttat gtccaaacta
    57121 tggaaagtgg tgatattgaa ctgccagaag agattgtttc ccgatccagg gctgtacttc
    57181 ccgcttcact tttaaaccta gatgtgaagc ttatctttgg aaccacagag atcttcatac
    57241 ccgttgtttc atcgaagaat tcagagcttg agcagacact tgtggaagat acttcagaaa
    57301 acgcattcta tgtcagcaag ggcctgttcg atctaataaa tagcggagag gttaacggct
    57361 atggcaattc ttcaccggtt gtgcttccgt ccctttacgg tctctccggc atcgttgtgg
    57421 aagaagaagt cgctgccgag gggctattga tttttcacgc tggagatgat ctggttgagg
    57481 aaatcaggtt atttgcctct gaagccggtg tagctgaggt tgtagatcgt cgctcattcg
    57541 aagctaagat tctcaaggag agtcagttca tgaatctcat aacctcgatt atcatatatc
    57601 ttcaattctt cgtagttgtc attattgctg ttgtcaccgt cacaaggata gttctgggaa
    57661 gacgaagaga gctggctatt agaaacatta ttggtcaggg aaggggtgaa atagctctgc
    57721 tgcttttcgg ggagttgatt ttagtgctat tttcaggagc cacaatcggg tttatcctgg
    57781 gaacaatgag ctggagtata tttaaggcat ttttcctgtc tggagtgtac atttcatcat
    57841 tcgtggcagc taaggttttt cttaaaatct ttataatgat actaattgtc ttgttttctg
    57901 gtcttgcgat ggaaaggtat ctgatatcca agcaggatcc tttgtcgatt atcgaaagat
    57961 aggtgtagaa atgaggttta gagaactggt tagcccaccc gatgcttatg agacaggtcc
    58021 gttgaacaag attactgatc ttgagggaat agaggtagga catttcactt tgattgaaga
    58081 cgcgccaaga tgtttgcgta ccggcataag cgtcgtaagg atttctgggg tatatggatc
    58141 tccgattcca gcggcgtgca gcgttttcaa cggttatggg aaatcgatgg gccttgtgca
    58201 gatagaggag ctcgggactg tagagagtga catctttctc acaaatactc tgtcgatagg
    58261 ggctgttcag cagggagcag cgaaactagc acttgaagac aatcctgaaa tgacatcttt
    58321 aaatgttgtt gtaatggagt gcaacgatgg gtacctcagt gatattcgct cattatctat
    58381 cagagaagag atggtatcta aagctgtaat ggatgcaaga aaagactttg ctcttggtag
    58441 ttgcggagca ggaactggaa tgatctgttt cgggtataaa gggggaatag gatcttcatc
    58501 caggattatt gaattcgatg ggaggaagta caccgtcggg gcgattgttc tttcgaattt
    58561 cgggaggtct tccgatctta gaataccttc gctagagttg tctaacgaca gtggtagttc
    58621 agctcaaggt gccggatccc tgatcatgat tcttggcacc gaccttccct tgatgcccca
    58681 tcagttgaag cgggtctcgc gacatatgag tcttgccata ggcttgcttg gggctcccgg
    58741 acatcacgga agcggtgata tctctcttgc tttcagtact gcgagggagt actctttgag
    58801 ggatcagcga ttgatgaaaa atgagaatac gattagcgag atattcaggg catccgcatg
    58861 ggcctgcgtt gaggccatag ttgattcgat gctttcttcc aacagtatga ctggcttcaa
    58921 aggatcggtc aaatctttga agagcgcagt gattcgcaaa ccttgaaatt aatctaatcg
    58981 aagtctgtcg aacactttgc gcggtaacct attggtgcta agattgaata ttcatttatc
    59041 gggagatgaa aatgaatgtt taaaactcga gacctaaagt cgaatgaagt tctggagaag
    59101 cgttcggcaa gattagttcc atgaatatcg atggctaccg tgatttctta tcgcttttaa
    59161 ggaacggaat cattctgtgt gagttgacat tcgagggacg ctgcaaagtg atcgatgcaa
    59221 atccagctgc gcttgaaact gaaggtctta cgagagaagc tgccattgga atggaccttg
    59281 aacacgtgtt tcctccggcc cgggaattca gtgtaatgga cggcgttcgt agagtaaacg
    59341 aaacgggcat tccacttcac attggatcga aacataatcc agaaacaaga agcgaggttt
    59401 ggagagatgt atcgatttcc agactttctt caaatgttat cgccatcgtc tacaatgact
    59461 tcgacgaatc atctgaagcg aggaaggacc tgaagaagag caaattagag ctagataggc
    59521 tgttttcaaa ccttcccgga attgccttcc gatgcaagaa cgacaaagac tggactatgg
    59581 aagttctcag tgggggattg gttgatcttg caggctatag accggaagaa ataattggaa
    59641 acaaaaaact gtcgtatgcg gatctcatct tttcggaaga cagggagaaa gtctggaaaa
    59701 gcgtacagga gtcttttacc agagacgatc attatgagat tgagtataga atagtgacca
    59761 agtcaggcat tgtcaaacac gtactggaaa ggggaaaagt aatcaaaaac ccaggtgaag
    59821 atcagtcgtt cgtagagggt ttcatctccg atataacgga tcttaaggct gcaaagatcg
    59881 agatcgaaga aagcaaagaa agactcgaaa acactttcaa gagtactgtg aatgctcttt
    59941 cgaagatcac tgaaatcaga gatccttata cctccgatca tcaaagaaga gttggactcc
    60001 tcgctgaagc agtatctaga aaaatgggac ttgagagaaa gctttgcgaa aacatcagaa
    60061 taagcgggct cctccatgac attggtaagc tttggatccc ttcagagatt ctaagtaagc
    60121 caggtagact caataaaata gaaatggaaa tgatcaagga gcatccacga cttggttgtg
    60181 aagtattagg ggagatcgag ttcggtttcc cgattgcaga atatgttatt caacaccacg
    60241 aaagaatgga cggttccggt tatccaaacc gattgaaagg tgaagaaata tctctaccgg
    60301 caagaataat tgccgtttca gatgttgtgg aagcaatttc atcacatagg ccatacagac
    60361 ctgctcttgg actgaatgtc gcaatagagg aaatcacatc cggagccgga actctttacg
    60421 atccttccgt tacaaggact tgtgttgatc ttctgaaaaa tggtttttcc ttctagtaga
    60481 ttactgggag gggatcgcct ctatgatgtt tctctttata gaaaacaggg gaatccttct
    60541 tcctgtgaca agaacctgat acgtccttcc cacttctagc atcctgaaga ccttagacgc
    60601 agcataaaag ccctggcttt ctgcgtttct cactgtgaat tcctcgcctg actcggagat
    60661 tattctgtag tcggaatttc ccgcgcttga attgtctatc ttttttatga cggtaatctc
    60721 agtcatcact accgctttgt caccccaggt aaagaccata aagaaggcaa gcgccaccag
    60781 tattgtaccg agtactacaa agatctctcc agaaatatct ttgagtcttc ctttcaaaca
    60841 aatcactctc caacttgcta cgtctaaact ctttcgattt tccaggctgc tttcctgagt
    60901 ctattcatta ttgcgagacc gactcccgtt tccgggaaag gctccgctat aatttgagag
    60961 aagctcatgt tctccttatt tctgagctct ttgaagagat ttgatgcgac ttcaagaagg
    61021 ttacttctcg aaccgagaac caccttcttt acttgaacgg gataatagtc ccggagctct
    61081 tcggaacaga gcacaagtga gctcttcctt ccggcgatga tccccacttt gctgacaacc
    61141 aagcccaact cgccttccac aagtatcaaa ggaatgtcag gtgagtagtg acgatacttc
    61201 aagccaggag acaaaggatc accgagatac tgctcccggc cagatgcaaa gcccggtatt
    61261 tgtagatctg gaagtacgca tttgagctga tctggatcta tcggcccggg tctcaaaaga
    61321 acaggtcttt ccctcgaaag atctatgata gtcgattcta gaccgaagac tgtcgacccc
    61381 gaaaggataa tacagctgac tctcccatcc atgtcttcta ctacatgttc ttccctggtt
    61441 ggactgggct ttcccgacaa gttggcgctg ggagcagcaa ctgggacaca agaaagctcg
    61501 ataagcttag aagctaccgg atgagacgga aacctgatgg cgaccgtgtc aagcccagcc
    61561 gttaccgagt cagggattct ttttctcttc ctgaaaatga gagttactgg gcctggccaa
    61621 acagtgtcca tcaaagctga gtactcgctg agattctgca aaacaagatc ggcgaccatt
    61681 tcacgactgg aaacatgaac aatcagcgga ttgtcctgcg gtcttccctt tgcatggtag
    61741 attttcgaaa ttgcagattc atctagggcg ttggcgccca acccgtatac cgtctccgtt
    61801 gggaagacaa ccaactcacc ttccctgata aatctcgctg cttttttcag tctgaactca
    61861 tccggttcct ctgaatttac tctcaaataa actgtattca ttcatcctcc gtgattcaaa
    61921 agaacttgct gatgaatttc cagagccaca acattcccgt aatacataca ctgacaataa
    61981 accccgaagt gattggaaac cagaactcaa aaccatctcg gcttacatga atatcccccg
    62041 gaagccttcc cagactgaga cctgccttcc ggaaaatgaa taggagcaaa gcaatcgcca
    62101 ggcacaacaa gcccactttc gccaggagtt tctcaaaagc ctccggactt atcacacgat
    62161 caccttcctc ataaattata aactatttct ttgaggcaaa atgaaagggt tttgccgaag
    62221 gaatcgctct tgagaagagc gcaggataga ttgattgttg gggtgggatc atgaaacaat
    62281 cagagaaagt ccgccacttg atttcatcgc ccacgggttt cccccgtttt ctttctggta
    62341 gagtcagcgt tatcgtcctt tcaacttcgg tggttcaagt ttccctgctt gacaatgaat
    62401 attgtcactg cttcgttcat ttcgaatctg cgggttatct caatcaagtt tcatcatgaa
    62461 gtcggctgca tcggttctct cttgccaccc ctgcttcgca taaaagccac gtctatatga
    62521 ttctctgtct ctaatgttta tcagtctgat acaacagcaa cctctttctc tcgcttcatt
    62581 tatggctctt cttagaagtt cactgccaaa tccctttcct cgccagacag tcttcacaaa
    62641 aagctcgcca acgtaccctt caggcttacc catgaaaagg tagggcaaga aatggacgct
    62701 cgtgtactcg gttaactcgc caccttcttc cgaaatgaga atcaggtgat catctctctc
    62761 gatactccat ttgatcattt ccttcacact tttcagataa atctcttctt caacattctt
    62821 cattttgaac catccgatat caactaatac ctcgtgtaga ccgggagcat ctgtctctct
    62881 aatctctctt atgatcattc tgatcacgcc cttttacatt ccatttcaag cccattattc
    62941 catagacgat actgcacaaa cattcagtta atccttgtgc ggagaatcca tttcatcatc
    63001 taaagtcaag agctagtact gtgaagatct tgaagaacgg agaattcggg attcttttcc
    63061 agcaaacttc tatccgatga ttgtgaatca aatatcttga aaaagagatt tcttacggat
    63121 actcctttct tcactgccca ctaatcgggc cttctctcaa gccaaaaaac ctgaactaac
    63181 tcttatccgg ggtcgagatc ttttaagaag taagagcaaa tctaaatgat cgtaaaaggc
    63241 ttaatcagcc ttatttgaag aggcttgagc catctagctt gtattgacac gaccagcgca
    63301 ccatgataga atgaactctg tagggtggag agatggccga gaggccgaag gcgcgtgcct
    63361 gctaagcacg tgagggttta taccctccga gggttcgaat ccctctctct ccgccattat
    63421 attgtttatg ggtgcccgta gctcagctgg atagagcgct agactgcgga tctggaggcc
    63481 gggcgttcaa atcgccccgg gcacgccatt aatcctttct atagaaccat ttctttcagt
    63541 ctctccataa tttccttggg ctttgaaatg ccgtaaatgc agttctttgg gttagcgacg
    63601 aattcttttt cggtactcac acttccgata aacgctccac ctcgaacgtt ctgagtacca
    63661 gtgtcggcac taaagcctcc caatacgccc ctgtctcccg aacccattcc agacgatgtt
    63721 acaggtatca catgacctac tcccaatata agttcgaaga aattcctttt aagcatcacg
    63781 tttgtgatgt gtctggctac gatcatatgg gaattcgctc tgaacattcc ataagttacc
    63841 tctactcccg catcgctgag attgtacttg aaggtagttc tatagatctc cacaaatact
    63901 atgccaagaa gccctgctac gatgaagagc agaaccggac cgttatcaaa taggggcttc
    63961 aaatcattgt aagtttcttc cgggaataag tacctaacga gccatccaga agcgttcaag
    64021 attgccggcc agaagaacca accgaatttt tttcgcgaaa acgcaagcaa aatgcctgga
    64081 atcacggtca aggcaatcca gctcactatt aagaacagcc agttgtttcc atcaattcca
    64141 gagagatcaa aattgagata gaaaacgatt cccagtacga agtagtaagg gtaaatgaag
    64201 tatctgatca tgaaagagcg acgcgttggt ttaagatcca tttttaccgc ctccggcttt
    64261 tgagatcatt tgaaggagct ctttctcgaa gctgtcagag tcttccagca ttatcttttc
    64321 ggttttccca tctctgagaa agagaagagt agcttcaatg ggtgacaaga gatctttaag
    64381 aatatacagg tacaggttca tttgaaaacg atacttattt atgaatctct tgttcagact
    64441 ggcaaacttg aaatctacta tcctccattt tccatccttc aaatatagtt tatcaacaat
    64501 tcccattaaa acgtactttc cgaatccatg ctggaagtga aattcgctga agactctttc
    64561 cgccccctct atttctttta tcagcggatg agctaccaga gtggaaagaa cttttttcac
    64621 ttcagacaga tcatattcgt tgaagttaat cctgtcaact gagactggcc tacccccgtt
    64681 aagaattgat tcgagagtcg tgaccgagcc gcgcttactg atgactccca ccggttcaag
    64741 caagtaatga gcaagaaggc caatatctgc ggcattcttt acaaggccgg ggcttccggc
    64801 gtatgattcc gattccagtt caaattcgtc gtctgcagtt atcgaagttg gagagaggta
    64861 ctttatgtac gaagagtctg tcagaggcag taccgaagtg acttcaggta acaaaggttc
    64921 ttcaatgtaa acagtctctt gaatggatgg aagatcgatt ggatagaagt gttttactat
    64981 cccttcaaag gaagcttcaa ccttatcgct ttccggttga agcaaagaac tgcatatcat
    65041 cttcgaccat ggtcgggagg aatcactcct accatttaga ctgataatta tcatctcacg
    65101 tgctcttgaa agagcgacat aaagagtgcg cttttcttct tcaaactcct tctcctcttc
    65161 tagcgatgcc aaacttccga taaggccttc ttctctgtca ggtttacttt tacttatgag
    65221 aaagcctcca tcatggaaga aaagtcgcct gtcagagttc ttctttttcc agaaagtgtc
    65281 acctacaatc acgattggaa actccagtcc ctttgatttg tgaacggtga gaatcttcac
    65341 actgtctgat tcctctgttt caagagcggc ttccgattca tcgctggaat ctataaaagc
    65401 cttgatgttc gaggagagct cgcgaagcga gctacccatt ctatccagat ccttcgcaag
    65461 ctcaagaagc tttttgacat tggcgatcat tctctctccg gatttcagtg tggccagctt
    65521 aggcaagtaa tctgtttctt gaacgaactt ctcgaggatt tcactcggtg acagaatgta
    65581 cttaagtttt ccgaatttct tgagcagatc aattaagtgc gcaaatctgt cctctttgct
    65641 ttccatgagg gcaaagtaca gtggcaaacg tctgagatct cttttcgacc ttagtgatag
    65701 gacttcatca agagaagccc cgaaagccgg cgaaagcaaa aatgacacaa gattgttgtt
    65761 gtctagggga tctaccagga gatcgagcca tgacagaagt ccggagacct cgggccgttc
    65821 gtagaagttt ctgcttccaa cggtgtagaa gggaatattc aatttttcca gtgcattctc
    65881 gtagctagag atctttgaaa aggtcctaag aagaatagca atatcaccgg ggactattcg
    65941 ccgtttctcg tattcaccgg tctttgtttt gaaaacaagc tcctcttcaa ttagggccct
    66001 tacggatctt gcgacctctc tcgaatcgtc agatgattcc gttcgaatta tcctcactct
    66061 cgaagactca atatctcttt gatatggtag agactctatg tcttcatcgt aaatcgaacg
    66121 aaaaaactcg ttttcgaaat ccctcttcat tatcttccca aaaagcttat tctgaaaggt
    66181 aacaagatcg ggatgtgaac gcctgttgat tcgtaggttt cttaattcgc ccccgttatt
    66241 ttgaaagctt ttcattgtct tgttgaatac cgatacgtcc gcaccgcgga aacgatagat
    66301 agactgcttc gaatctccaa caaaaaagac tctattctta gtctcttccc gaaggagtcg
    66361 aatgatctcg ctttgaagct catcggtgtc ctggaattca tctacgaata tgaatttgaa
    66421 gcgatctcta tatttctcct ggacttcggg catcttgaga agcagatccc tcgtttctgt
    66481 gagcaactga tcgaaatcaa ggaaccccga tcttacagtc ctgtctctgt aatcggaaag
    66541 gatttcacta taggctgccg caaaagactt cgaaccctcg atgatttcat cgtcacttat
    66601 gattgagtca cctaagagac ttccgacagg agatggattc agtgccaaag aatgtcgttt
    66661 atttgatatg gcttctttca caattctaaa agccttgtct aagcccgtaa gctgaatcaa
    66721 aggttccagg ctttcgaggt tatcttcgaa atatgttcgt acaacctttt cttcaagcgt
    66781 cgctctcctc attccgctta gaatctggaa gcccggatct attccagcaa aaagggcaga
    66841 ctccctcaat atcctttcgc agaaggaatg gatggtggaa atccacgcgt acgacatttt
    66901 gtttcttaag ggtttccagc ttccgggatg cccggaggct attctatgat cgatttccgt
    66961 tattactcga tccttcattt cccgtgccgc cttattagta aaagtaattg ctacaacatt
    67021 atgaacatca agccgttctc cgattctgaa tgacctgtcg aagaccccga cgtactcctt
    67081 aaccagtgaa aacgtctttc cagtgccggc agaagcggtg acaaaaatgt ctttattcat
    67141 attcttttct gccttctctc caggttatgg acctctttat ctcggagaat ctgcagctgt
    67201 agcacttact tgcagacact gtgaatgagg gagtgaactc gccagaatag attccctcgt
    67261 gtacttcctt taaccatttg agaagatcag actccttcac aatcctgttg tcctttttct
    67321 tacccgcgaa ttcccagacc ttgcctcctt ctgaagaaat ggttctgaag gcagctttgt
    67381 tcacggtttt tccggtcaaa gtcttgaata ctcctcccgc aacatcaagg ctttcatctt
    67441 cgagaagttt ttctgcaaca agcgaataga ggagcagctg tctttcatcg ccgctatcac
    67501 cgcgcttgta gtctattaga tagacagagg aactggatct gtcgatatcg actctgtcga
    67561 tctttcccct caagtaaagg ttttcgagta gcttcactgg ctcagtttca cctattccga
    67621 aggccttctc gaaggcgtat ggaatgaaat tgtctcccat gaaattcggt ctctttcctt
    67681 ccctgactgt tatgtactcg tgaagtactt cgctcaggcg ttcaaattcg aatctgaaca
    67741 cgatattgct atcatgcatc aggtgcctct tgatgttctc ggcaagcgaa tcctgccagt
    67801 ctctattctt gcccgaaaag aagtccttca acactgaatg aaagatgttg ccctcatcaa
    67861 gaggcgtcaa ttcaaagacg tattcagggg aaacatccag accaacaaca taagaaagaa
    67921 agaaggaaaa cggacagtca tgataagact tcagtcttga gaaggagaag acgttaccga
    67981 caacttcctc taaacgcctt ctgttctgga ctgaccaact gaaatctctt ctgaagttcg
    68041 agaggacggt atctaaattg ctcagagggg gaaagctctt cagaagatcc catttttcat
    68101 tcaagaagaa cttcacagcg gagatcttca actcggattg gctcatagat gcccggagtt
    68161 ccggaatgta tccagatctt cttcccacct ttcttccttc tctagtggtc tcacatcccg
    68221 aactctccgc cacattcttc aggtatggag aaggtagaat tggttcaccg tcgatcgtcg
    68281 attcaggcag agagaacctc actgattctt cagcagtaga aatcgacaga tacaggttga
    68341 gcctttcgtg tgtttcctca tcaagaagaa ggtctctggg tcttgaatct ccaaactgcg
    68401 tgaaactgta caaaggattc atttctacgt gcggataagc accgtcaaca aacccaacga
    68461 agatcttcat tcttttcttc gagaagcgcg aattgatgag tgactgtatc tcaaccctgt
    68521 ttgccttgtt cctcgaaaga ggaaacttgt catgcttaag tagaagcatt agatatctgt
    68581 agtattctct tggggaaaca gaggacttcc ccatgaaata cagaagtctc tccaggtaag
    68641 gtagggtatg ttcgaaaaac ctcactattg catccatctc tctgtcttta acttcactct
    68701 catacccggt tagactttgg atcagagaaa gttctttttc ccaagtaatg aacattttcc
    68761 gataattacc gacatctcgc ttacgcagac ttcgaaaaga gtcgaggagt ctgaagattc
    68821 tttgaactgc cggaactact tcgctgtcaa ttctttcgat cgtctcatca agctcctcag
    68881 ccgcgctcat ttcgagctca tcgtcggcaa gggaaattat tgcatttctt ctttctttca
    68941 tgaggtcaac gagtcttgaa agcctctcat cccaggaact ccgtctcttt tccaaggaga
    69001 gccacatatt tggtctctcg tagataattc ttgacatagc agcggcggat tcaataaaac
    69061 ttgaatcaac ttctcccccg tagccgctgt cagccatagc gattatcttc tccggtggga
    69121 atcccagaac cgcggtttca agtggcaaga ttaaccgtct tatcgagaga gatgagtgaa
    69181 gctcttcgtc gccttctatt ctgtgtggaa caccgtattc attcagctta tcggaaagca
    69241 atttgctgta aagattgaag tcggatgaga caatcgaaat gtcacctggt tcgcaggcgg
    69301 aaataataga ttccttcaca aatctcgata caccttcgac ctcggcgaag atgtcaggat
    69361 agaccacaac actcaccgaa tcacagttct gctctacttt acctggagag aacagggctt
    69421 ctttgaaccc gttcagaccg cttgaagaag aacttttctc gaagaatatc cttttgattt
    69481 cgagatctgt gctattttcg gctatctcat cgatcgtctc aagtatcgaa tctatgtttg
    69541 aaaagagact ttccttgtca ggttcttgag taatggtaat gaaaacttcc ctgtatgttg
    69601 gaatgatggt cttgaaaaag atcttcaggg ccggactgaa atcatgaaac ccgtcgagga
    69661 aaagagtgtc cccgaattcc tcgttaatca actcatcctt gtgctcttca atcctcgtat
    69721 atgcgtcgaa tgtgtcaaag atgtttcttc tggagaatct actgtcaagc tcgttgtaaa
    69781 ttgactctag aacggagact gcctcgtctt ccgtagagaa tatccggtcg aaccctctat
    69841 tctcttttac atcgtgaatc atttcaagaa ggtattcgac gaatacgggc gagtcggcga
    69901 gttcgccctt tcccatttga tccagaatgt caccgatctc caacctcatc aaatgattcc
    69961 caacgtgaat agaattcgga ttcagtctac ggaaaacctc tacggcaaat tgatccattg
    70021 cgaggaatct cgaagccggg atagtaccca ctcgcgatat gaaattctct ctaaagtatc
    70081 ttacgtggtc gccggaaggt cccacgaaga agtaggagaa cggatctctc caatggacgg
    70141 tctcgagttc tcgaagcaac cgctcagtct ttgctgaggc taaaggacct atgaatagcg
    70201 taatcttcat cttactcctc ccacaaattc gattctatca tccgctgagt gttgtggaaa
    70261 atcgctgagg atttctatag aaacacgtaa acctaggcac ccacgaagcc cggaggaaat
    70321 ccacgaattc tttcgttgta aagatagttg atttttagag tgcgcgcaga tcttttagcc
    70381 tctttgaatc aaatctcaaa taaactgtca tcgaaggcga agtagaacaa gtcattaagg
    70441 caaagttcgt cagaaaaaca aactcctctt ttaatcccca aaggcctcaa atggaagaag
    70501 agacctcttg actgacctct gtaggaagag gtttcgagat ccaaaaatcg ccgcggttga
    70561 aatagccgct agagatggaa tatagacgac gctctccccc gcaactctgg ctttttcata
    70621 gtacgcaatc acttgatttt ttgggtatcc ttccaattaa gagtatggga tttaccggtt
    70681 cctaatcatt atcaacgaaa gactgctttg atctcgttca tcagagttcc tttttacaga
    70741 tctctgttta gtgaagcaat tgtctttcgt tataagtcat cctagaatac tagatcaact
    70801 cttaaggaag gtgtttagat accgatttct tttcctaatc aatgcggcca actcttaaca
    70861 cttctgtcac ttctagattc tcccctcgcg aatcagagaa aaagaacgta ttgctagaca
    70921 tagtgcaaat gcgaggagtc catgaatatt acatctgtag tagtcaatca gccgtccgtg
    70981 aactcttctc ttcgatgttt ttggtatttt ctgggggatt cagtttgacg ataaaaaaga
    71041 gtacgagcat cgtcattcca gcaacagtca atgccatacc tactcccaac ttgtccgcaa
    71101 ggaatccaac tatgggcgct attattgcca ccagcagagt cttcaactga gattcgacag
    71161 atagacctga tgccatagtt ttgtggtcta tgttatcgct tatgtagctt atattgattg
    71221 gtcttctgaa attctccaga atgtagagca ggaagaatac tatcactgcg attacttgaa
    71281 gagatactat ttgaaataac ccgctcagta tgactaatat cgccccgaat atataagagt
    71341 aattcagcga tctcgcctcg tttcccagtt ttttgctcaa ccttccggca ttcttagaag
    71401 catagcttgt ggcgatgaag atcacgaaat atacgactcc tacgattacg gaggttctct
    71461 gctccgggct gaagccaagc attatcggaa gaccaagggc gagagccttt agaattggct
    71521 gcagatactc cttgatgact ttgaagaaag catcatacag tgacgagttg agaacacctt
    71581 tgagtgcatt ggcacttctg aaaatcccca ggaaggcttt caatgtttct cttttctccc
    71641 cggatttcag ttcaccatca agttccttgg ggtacatcat caaattcacg aggtttacgg
    71701 cataaggcaa tactgaggcg agaaagacta tcttgtagct acccgtgtaa aatacaagga
    71761 aggcagctat caaggcgttt attgctgaac ctagttgtga tgcggctcgc gttgcgccat
    71821 agtagtgaac cttaatatcg gtcatatcat taattctcag gtactcaagt atcattgctt
    71881 tgtgagttcc ggtacggaag gcttccccaa aggcgaaagc tatcattgca accatatata
    71941 tgtagaagtt cggaaagaag taaaagacta aaaaagaaga aagataggag atcatcgaaa
    72001 aaaccatgga caatcttctt ccaaacaggt cagcgaatat gccggtcggg agttccagga
    72061 agttagtcgc tacttctcgc acggagacaa gagtaccgat ctgaaggaag gaaagtccca
    72121 tttctctgaa gaacaagata agaaaagggt cgaaaaagcg aagattctta aggaacccgt
    72181 aagctgcgaa tctatagaac tgcccgtccc gttttatttc catttgaaac ccctcccgaa
    72241 gagttcaata gagaaattat actctaagaa aactctccgg gtccggtgat ttcactttat
    72301 caggaaaatt cttaagatta gagggatcag tatcattgcg ctcatgaccc actcccactt
    72361 ctttgcactc ttgtacgcgt tataaataag cacagaagat aaatagactg ctatcgcaat
    72421 atacaagtag gtcaagttac tgacatctcc tttctcttgt ctactaagag aagagcaaca
    72481 gaaacgacta caagtccagc aatgaagagg ggatagaaga gccagctgtt ggcgaaccta
    72541 cctatttcgt ttgagaatat tatggttaat gtggcgaaaa tcattgtaat gaaaaaggca
    72601 ggcattgaac tgagccagat tttgaagtaa ttcttctctc cgacgacatg ggccgcaaat
    72661 atcccggcaa gcgcagttgg gggtaccatg tcaccgagcc ccgcgaggag ggagagcgcc
    72721 gaacagacaa cgatgtcgtt attcccgaga aatgcaagca agaacggaac tcccagcact
    72781 gaggcagaac cataggaaga cactgcaccg aaagcaggga tgccaagtgc aataccaacc
    72841 aagagcgcgc cttgaggaag accgttgaga aatcccacaa ttaaacccct cacgccgttc
    72901 agagtcataa tctgaataaa catcccgact ccaatgagaa tcgagagtat accaagagag
    72961 ttctgaaccg ctgaaagtga gatcttgaaa agatttcccc ttgctccagt caatgatgca
    73021 acaattgccg atatcatgaa gatcaaggga agaccaagcg aaaagcccca actcacgttt
    73081 tcaagcacca tgagtatcac cattattatt aacggaagat acaccgcaaa gccctttatt
    73141 ggctcccttt ctcttgttct cacgaactcc tggagatcga ccggtttaac ctgtctaact
    73201 ccaataatca gagaagtgac aattgccagc ggaaatgtca tcagcagaag agggttagtg
    73261 aaaccgatat atggcatgtc gacaccctga ccgattatca ttgcgggtag atttattggc
    73321 ggggcgatca ttccatatat accgctcatt gcgattatcg atcccgctct ccttttgcta
    73381 agccccaggg tcttcagaac cgggaaggcg atagcacccg ttgtaagaac cgtagccgtt
    73441 gatgatccag taatcatacc ggctatcatt atgaagaaag tcattagcac gagaagaaaa
    73501 gtcggtttct tgtgaaaggc aatcgtcatg ttgcctgcaa tcgtgtccag aagacccgat
    73561 accttgattg attccataaa aatcattgct gaaccaatca caagtatcgt gtcgatgtaa
    73621 ccgaatcctc cttcaacgag atgcctcaga gggaagaact cgccgaaagc aattgaaccc
    73681 gcaagggctg aaaacatcaa tgaaactgat attgggattt ttattattgc cagacctacg
    73741 aaagttgcaa gcattaccaa aaaataccag acttcaggtg tataaatggc tgtcaccccc
    73801 ggtcattcag ttcaaaaatg agaatattcc gttctgcatt acttcttcat aggtaggtaa
    73861 gttttcgacc tcaaccgtct cgccaatcat catttcatat gcactgaaca aggatctgat
    73921 tatctgaagc tgaagaccgg ccctgtgagt caatgggtaa tcgggatcat caataacatc
    73981 gtacttgtct ctccagttat cctgcccggg atttggagtc tctgaaagga atgaaagcga
    74041 acctgttgcg tcacggatct caaggtgact taggcctcta aaggctctgt tcgattcttc
    74101 gagtttcata gatactccat aataagcctc gatttccatg atcgccactg cacctatttc
    74161 cagagcatct ggatgacaga ccagcgtata ggccaaactg ctaccggatc gttcattccc
    74221 ctcgctgtct atataactgt caggtgtccc gctttcatgc atatctagat tgaagtctac
    74281 attctcagtg tctatcagct ggataattgc gtaagcaagc tgctgcgtgg gattgccatc
    74341 tgctactccc ggatactgcc tgttcagatt tctcgcctct gctccatctt caagagtgaa
    74401 atcggattgt gcatgcttga agatttctgg atcgggtatt ccctgatcca gaggatcagt
    74461 tcttctatct ccgtacacaa gaaatcgagg accggattta ccttctatgg ctatgaaatg
    74521 gggtgcgtgc cctgtctcat cttttatgga ggcagcgctc aaatttgaaa acgggatcac
    74581 tatcacctta cccttcgtga cgatcacatt ttctatgaaa actattgccg cagttgttcc
    74641 cgaaatttcc ctggcatgag taccacccat gagaacaaac gtaggaccct ccactccgct
    74701 gtccatgaaa tagaccgggg tgtccatgtt tgtgtttctt atgggagaaa aataatccga
    74761 caaccagcca atatcagtta ccccataacc tggacggatg tcgggcctca aaaaagctcc
    74821 aaatacagca gaagtagata agagaacgat gaagaaaatt gctttgattt tcattagtct
    74881 tcctccgatt aagaataagg ggcgcaggct agcgcccctt caaaatagag ttatttcccg
    74941 aagtactttg tcaacacgtc tctcagatca accgtcttct caaaagtggc cataggaact
    75001 ccggcctctt cgcttagagc cgtgaagaaa ccatcttggt cagcgtctga ttttacaaag
    75061 tatgcgtctg caaaaggtgc aagttcggtg actattctgt cggaggacgg accccttcta
    75121 gattcacctt cgagattaca gattactact ttgaggccgt tttctttagc cgcttcagca
    75181 agggacttaa ctctcagaat ctcggcctca atgtcgatat tggctgcgcc gagacccttg
    75241 ccgcttgctc caacaactaa cacaagggtc ttgaactgag aaatgtcgat agaatcaacg
    75301 ctgagaagct tctcaaaagt gtattcgagt ttaatcatct ttgcaacgac agtaaattga
    75361 agtgcgccgg gagactgccc tgcagatgta accgctaccg gttctccgag aatcagtccc
    75421 tcttcagttc cgtcagccgc aaatccgaca aacgctacgc aagcaataag cagtaaagaa
    75481 actagaaacc ttttcaaatc acatcatcct ttcaataaaa tcaaaattcc ggtgccgatg
    75541 cagatccgcc tcttctgatg gagccggctc caatcataag accgttttct tctacagaaa
    75601 taagatttgg gccgccaaag aaaagatcgg ggtatgaata tacattcagc ttatgtccaa
    75661 gaaccttctc gaggaagtca agtgtagttt gcggatagcc ttcttcaatt ctgagatcct
    75721 tataagtagt gtatccaacg aacttgggtg ttcttactgc ctggtccaca ggcatgttga
    75781 aatcgattag atcaactgcg agctgaacca ttgtcgatat tatccttcca ccgcccgggg
    75841 tgccgattac ccagcgaact tttccatcct tctgaattac agtcgggcag atagtcgtcc
    75901 tgggtctcat tccacctgta aggttgatta cgtccccttc tctgtatgag gaagcgaagt
    75961 tgtacatttc gttgttgaag aagaaccctt tgaagtaaac acttgtacca aagaagctag
    76021 agatcgtctg ggtccaggct acggcgtttc cgtccttgtc cacgatcgag aagtgagtcg
    76081 ttgacggaga ctcttctact ccgctttcgt tagctgctac cgactctaag gcacccagaa
    76141 gaacctcttc ataagttgat ccatctgcaa atggataggc gtctccctga tagtcgtagt
    76201 aagaagctgg atcgaaggcc tgtgacagat cgatcgccat gaatctggtt ttggcatatt
    76261 ctttgctcat aaaggcctcg taagggagat cgtagaactc cggatcgctt atgtaagctc
    76321 ttctatccac atctccgagg accagtgcct gctgaataat atggactgcc agaggatcgt
    76381 cccaggtcat agcttgaaga ttgtagtttt cgagagtgtt gagaaccgca atcatctgcg
    76441 gaccggaaac gggcgggtgc ggaacataga aatcgtatcc cctgtaagtc ccatgaagcg
    76501 gctctctaac gatagccctg tacatctcga gatccgactt cctcataaaa ccgccgttct
    76561 caatcatgaa ctcttctatc tcatctgcga ggcttcccgt gtagaatgta tcgatgcctt
    76621 cctcttttat cctttcaaga gtctctgcca gctgaggatt tgtgaatgta gatccaactt
    76681 cccagacaaa gccctcgttc aagaaatcga agccgttttc gagtaccagt tcgtatttgt
    76741 cggaagttgt ctgagcaaat gtttcatcga ctatgaaccc atttcttgca agatcaattg
    76801 cgggctgaat gagatccgcc agaggaagtg ttgctcccaa cagccaggct tcttttattc
    76861 cggcaagaac tccgggaaca caggctccct ttggagtata atttgctttg cttagggtca
    76921 gtccgagctc agagagagct tcgtaagagg cagccatcgg agcggtgctc ctgaaatcga
    76981 tagcgtactt gcttccatcg gccatcgtga ttacagcata gccttcaccg ccaagaccag
    77041 atgcgtaagg ttcgactact ccaagtgcaa aactaacagc tatcgctgca tctacggcat
    77101 ttccccccat ttcgagaatc ctcgctccga cttcggcagc atacttgtca acagctgcaa
    77161 ccattccgtt gcttgacgct accgtcggag ccgtgaccgt ggcgaatgtc gctgtgaaaa
    77221 caaacgtaat aagtgttaca agcagcacta gtttcttcat tctttcccct ccaatctaga
    77281 aatgtctgtt tctcaagaaa ctggattaag atacgtcccc agaccgtcaa ctagcccttc
    77341 atagggcgga acgtcagaaa gaaatattgt tctctctggg aacatcatat tccaggagtc
    77401 aagaagaact ctgatggttg caacgtgtct cgcaactctc agatcaagtg gataaccgtt
    77461 ttcatcatat ggagcaaaga gcttgccgtc ttttgaagcc tgcagatagt acttgtcgat
    77521 tccctcagtg acgagttcat cagttgtctt tcccctcaac ctaccttgag aggggtttga
    77581 gacttcgaga agaacgacat atgcatctga attgtctccg atctctctgt gagtcaaacc
    77641 tctcagattc actggagatt cctcaagtct aatgtctatg ccctcgattt caagcatcat
    77701 ctgagtcata attgccagtt cggccgcttt ctgatgaaag acgatcgcat tgttcactgg
    77761 atactcgggt gaggactcat gtagatcgca aacgagatct atgtcttctt caagaacgag
    77821 attcatgatc gctgcggcca ctttctctgt agagtaacca ttctcccttc cgggaaaggt
    77881 cctgttcaaa ttggagactt ctccccctcc aagagtggct ccagttggat gtagatatat
    77941 tgtcggatcg ggccactggg cgattggatt agttcttcta gaaccgaatc tgaagactct
    78001 ttccgatccg tccggtaatt ctattgaaaa gaactgaggg gctccttcca tagcatcgtt
    78061 gtgagtaaat gcgctgtgat ttgatctagg aatcacgtag actgttcccg aaagagctga
    78121 tacattctcg acgaaaacaa ctgcagcgag cgttccgccc ggctcgtttg ggtgagttcc
    78181 cccaagaagc aaaaacctac cgccctcctc ggcgcccctg attatataaa cttcggtgtc
    78241 tagcatagtt ccttcaattg aaggtagcca ttcactgagc attttgattt ctgtcacgcc
    78301 tgagccggga ataatcgggg gatcttccca catggcggta aacgaaaagg ccgaaagtat
    78361 tacaacaatt gcaactgaag cggtaaaagc caagaatttg aaatgtcttt tcaagcgcat
    78421 caatcctttc tactccgaga gagaacctga ttagcaatcc aagcgatggt gattgtaagg
    78481 tattcgatac ttgatcaggg atccaggaga cttgtatatg ataataccac atagtaatta
    78541 tactacctta tcgtactaaa gttcgccttg gagaaatgtc aatccgtttc aaatgcaacg
    78601 aatcaaaaag tctatacaac caaacaaatt tgtgcagaga accgttcggc attcgcacaa
    78661 ctaagtctca cagcccttat gacgtgttca cttcgatctt cgttcgacac attgaattca
    78721 tccagataat gggagtatta gtttttgccg aaaggaaaac agtgtaggtc cctcgcaaat
    78781 taacaggtgt ttcaaattag atagcttggg attctcgatt ctggttttca tgactttttt
    78841 gttcagactg cttcttcgcc ccttgatttt gaaatcttca cggtttcgct gtttctcgca
    78901 ataattgtta tcaaagtttc tatgggaaac agtgaaggag tttccccgaa gacgatcgat
    78961 gtttcatcac acactttgct tccgatctct gtgacgtcag tgagcgcagg tgtactctct
    79021 gcaaattcgt aagcatctat tatgagcttg gatctcttgt tgccgccttg ggagccgaga
    79081 atgcggctgt cctcttggcc gtcgtgacgc cggcatcatt tgtagggatg tcaccacgtg
    79141 ataaacagaa agatgaaata tcggagcttt ttagcggacg gataataggg attatgttat
    79201 tctcagttct aaccactttg gaggtgcatg aggaaaggtt cgtactctgg cctttggtag
    79261 catgtttcca tcgagagttt tgagatccct tttcagaaag attgcggacg ggagatctga
    79321 atcgctaaaa tctacttcgt gaccagaagc gattcaatta tgtgaattac gccattgcta
    79381 catagaatat cgctttcaac tatcggaatg gaattgacta acaggtcttc tttcatctca
    79441 aagtacaacg gctttccgat tagagagagg agcatgttag agatcttcaa ttccgacagc
    79501 aggtactcat tcggaagaat gtggtactcg agtacctttc tcagttcatt tgcactgtag
    79561 aggaaggctt tatcgctctc ttttagctcg agaaaagctt cattagtcgg agcaaaaacc
    79621 gttaaacgct tattttcact caactcactg cagataccgg ttttcttcaa cagttccgcc
    79681 ataatatcta tctcgtttgc tttcagagtc tcttcaatcg ttgaaagctt ggtccttgaa
    79741 tcttcgtgaa acataacatg atcaacgata ttgatcattc cgttgctggc ttcgatgtcg
    79801 ctcagaatta caactgtttt ctggggacca attcgcggag gagttgtgcc taacacaata
    79861 gtttctccaa gaagcgatct aatagagcct ctctcaaaga gttcttcaac cgagaaggaa
    79921 cctttcacaa tatgaaaaag caatatctca ctcagccgtg ccggatctct gaaaaggttc
    79981 tccattgtct caggaggcaa tctggcgaat gcgacgtcgc tgggcatgaa tactgtatat
    80041 agaccttcac ctttaagaat gtctataaca ccagtttctt caagggaaaa gacggccgta
    80101 tctagaatac ctgtgttcct catgacctga acaatatccg gttcttcatg gggaaagatc
    80161 acagtatcta tgatgtgaat aacgccgttc gtggcttcga tatctctcac caagatcgat
    80221 gaaccgtcta ccttgattcc tccctttgtg tcaacagaaa gaggttgacc cagcagtgta
    80281 acaaaattgg gtctctggac gatgatgctt gaagagaatt taccttgaat tatatgatat
    80341 ttaaggactc tctgaagacg aaccacgtcc ttaatcaaat ccgctctggt cagttcaggt
    80401 agcttcatga atgcgctgtc ggttggcgca aaaaatgtga acggcccctt tcctgacagt
    80461 gtttgatcta acccggataa ttgaatgtag ctagcgacta tgctcaactc ctcagtctca
    80521 tttatcgttt tgagaatgtc ttcttgtccg agtgaaatca ccgatgacaa aatcagaatc
    80581 acgaaaatga gatttctcat cattattcac ctcagcttta ctattacgaa tatcaagagt
    80641 cgcaagagaa ttctaaaact tgtgaagacc tatcacgagc aagtatcgat atctaaacaa
    80701 aaaacgggag gcgggtagct aaagcccgtc tccccagagg actgacaggt tttacttctt
    80761 tggtgtttct ttcttttctt tctttacttg tttctttgct tctttcttct tgtccgccat
    80821 tctggacacc tcttttttgt ggcggctcga ggccgcacta tgtcaatttt agcaccgtta
    80881 tcagctagtt gcgccaagtc ttctaacagc tattctagcg aaaattgtct tcgttttttt
    80941 ggagcatgct ttatgagata actaaagtat tgctcgtttt tctgagtgat agatgattga
    81001 tcccttggaa attcttccga aaaaagctag atcaactaaa catgggatta gtgcggatta
    81061 cacggtcacg gaggaaattg cattgcctgc aagtataata tgtttatgta ggatcgagga
    81121 ggtaaacatt tatgagaagt attttaatca atggtgaggt ggcttacaat gctccctccc
    81181 agatatttaa cagtgtcacc ttcaaggaaa ttcttgccga gctgatcaat gactccgaag
    81241 aaaagaagaa ttcgctgttg tctcttttcg aacccttctt aagaaatacc gacacgatgg
    81301 agacttcaag aaaatacgat atcgacggta tcgtcgagct tcttcttgcg ttgacgatag
    81361 atcctattga gaaaatcgac ggctcatcct actattcgtt tcccaaaatg agcaacaaaa
    81421 gggagctcct tgtgaggttt gttgaaagcc tgttttccct gtggagaaac aagcacaggt
    81481 tcatagtcaa gagagacgaa tacacgccaa acagatttga caggatcttc aagcagatgg
    81541 tcctcgtaag aactaacact gatcttaaga gcctggtact gagcctctac aggcagattc
    81601 tgataaatgt atcggacatc aggctcaaga ttctcaggca ggagcccggc ggagcccagg
    81661 tgggtttcat cgtagacaac cccgaggtag ataaaagagc tagggtcccc ggagcagact
    81721 ggttgtacgg aatggcctgt gtttggagca ttgtcttcga acccccggtc atattttaca
    81781 caaggtccaa taagaggcat ggacttttcg aagtccgtga caaaccaatt ctaagcaaat
    81841 tgaatatccc gtcctcaaac gattggttcc tctttccaat tcacgtaggt tcgaaactaa
    81901 tcgacgttgt tgtgaataag gagtatcttt ctcatgcggc cgggctcgga aatctcttcg
    81961 agttggcaag ttttgatatc gtaaaaaaca agaagccaga tggaatatat attttaggga
    82021 tagataactc attcttcgaa aagaaagaag acttcaacgg agtcatatac agggaggacg
    82081 acggcaccta cattggaata gtgggagacg atccttctat agactacttc gggtatatga
    82141 aaaagatgat cctcactatt cataatcttc ttgttatcga cgaagggaga ctgccaattc
    82201 acggggccct tgcacacata aagcttagga atgggaaaaa ggccaacgta atgcttatcg
    82261 gagatagcgg agcaggtaaa tcagaaactc tcgacgcact gaatagactt catgccgaag
    82321 tttctgaagt caacatattg attgacgaca tggggtcttt agatattacc tcttcgggtt
    82381 ccgtgattgc ctacggtaca gaaacgggag cctttgttcg cctagatgat cttcaacccg
    82441 ggtatgcata ctccactatg gaccgtagta tcttcatgaa tcccaacgag atcaatgccc
    82501 gggtaatcgt tccgtactca aactacaccg atatcatcaa gcccacgaaa gttgactact
    82561 tcctgtatgc aaacaattac aacgaagaca aagatagcga tctaagaatc tttaaagacg
    82621 ctgaagaagc ggcagcggta ttttcgaatg gtgcaagaat ggctaaaggg acgaccacgg
    82681 agaaaggact cacttacagt tattttgcaa acccatttgg cgcgattcaa cggagagaaa
    82741 gacatgaagt gatagcagat cgttatctaa aagctatgat agattcagga gtaagggtcg
    82801 gagaagttag taccaggcta gggatccccg gatatgaaat ggaagggcca ttaatggctg
    82861 ctaaggcact actcaaagca atagatgaac agagctgaac tggtgaaaac atgaaataca
    82921 atactttttc gtgattgaga gtcaccaaat gagaggtggt agagttgccc aggaatgtag
    82981 ttgaagtgaa ggatttgaag aagtattatg gtgaaactaa agcagttgat ggaatcgaat
    83041 tcgatctgag agaaggcgaa gtactcgcca ttcttggacc gaacggagca ggaaaaacca
    83101 caacagtcga gatgctggaa ggattaagaa agccagatca tggaaccata gaatacttcg
    83161 aagaaagact tcctccttca agtgatagag tgaaggagga aattggagtt cagctccagt
    83221 catcaagctt ctttgagtat ctttccgtga aggagactct agatctcttc agaggtcttt
    83281 acagaaaaag cattccttcc aaggatttga taagagaagt ctctttgcag gacaaagaga
    83341 agacgtacac caagaatctc tctggaggtc agctccagcg attggcagtt gcggtggcac
    83401 tggtcaacga tcctagagtg gtgtttctgg atgaacctac gaccggtctc gatcctcagg
    83461 caagaagaat gttgtgggag acaatttcgg cgctcaagaa tggtggaaag acgataattc
    83521 taactaccca ctatatggaa gaggccgaga gactggcaga taggattatg ataatggatc
    83581 agggcaaaat catcgccagg ggaactctat tggagctaat agattctata gatgttgaga
    83641 acatagtcac gtttagcgtg cagggagata cccctattcc agagggcttt ctggaggttg
    83701 aaggtcttca acacacggag aaccgtgaat attcggtaac gacaagggat gtggagagaa
    83761 ctcttggaag gctctttgag atatcgaagg aattcgacat gcttctggat gatgtaacga
    83821 ttagaaaacc caatcttgaa gatgtattca ttacactcac aggcaagaaa ctgagagact
    83881 gagatgattc catgagcaga atgtggaatt tcacttcagg ccttttcaaa agtgaaataa
    83941 gagagtttca ggttatgttt tggagtttca tatttcctct gattctctat ttcattttaa
    84001 cttccgtgtt tggttctctc tatggagatg atactcaggg gataagcttc agagtaggaa
    84061 tcgtcaggga ggaatccctt tcaggtcttg gaatgatcct cgacaaagtt atagaagata
    84121 tttcctctga gaatgggccc tttgtcaaaa aggagtatgg atcagtagat gaggcgttga
    84181 tagatctcaa ggaaggtaag caggatatcg ttcttgtcat tccagaaggg accagcgcaa
    84241 aaatcgcaag cgccgctgtt cttcagactg gagaagtcgc tctgcgggtt cattatatca
    84301 gcggaaagga ctcgtcttcc attgcctcta atgtcctctc tgaaataata gacgaagtga
    84361 atctggagat aaggcgtcaa agtggcggag agtatgtcga cgttgttact gaggcgaaaa
    84421 ccgtttcggc gagagaagaa aaacctttcg attacaggga atacatcttt ccgggagtag
    84481 ctttgatgat gatcctctcg gtagctcttt tcaacagtcc aataggactg atccaataca
    84541 gggtatcggg tgtcaacaag aagctctaca caacgccttt gaaacccctc gaatatttcg
    84601 cgggtcactt cgccaagctg attttcacta tgctgatctc tttgacctta ctgtatttaa
    84661 ctgcgatttt tgtatataat gtgcgaggtc caattctgga tatcagattt attcttacac
    84721 ttctcttctc aatggtggta acgatttcct tcggactgat gatcgcatcc ttctccaaga
    84781 agctctccac tgtgacagtt cttgggcaga ctttatatca gctgatgatg ttccttggtg
    84841 gtctttactt tcccgtcttt catctcccgt ggggaattcg ctggttggtg tatgctctac
    84901 caacgaccta tcttgtcgaa ctgagcagaa gggtaatggg atatcagttt gcaccggttt
    84961 ctttgttctg gctgatctta gtgccaatca tttggacagc cttttcgacc gtaattttcg
    85021 taatcaactt taagaaggtg atgggctatg agtaggaaca gtcttaaacc ggtattcatt
    85081 gcctttgcaa aggaggcttt caggaacaga ttggaaatct tctttacact cttctacccg
    85141 atgatatttc tccttctgtt cggattcttc ttcggggggt ccgaaagtta tcaaaaggtt
    85201 tcagttggcc tgtatcagac gggaccatac gatatttccg ccgttattga ggaaagcgga
    85261 gcctggaatc tgcgtctgta cgacggagaa acggatatcg gggaagctgt caagaatgga
    85321 gacattcttc tcggtatgat attcaacggg gagaagatca cctatcttca tcaggaaggt
    85381 aatttagagc agcaaagcac tatcgaaatg gctcgagcaa gcatttcggc cgcagtggag
    85441 aaggaaatca acaatgccag gtctttcata gtcgtggaga atattcccga aacagccggt
    85501 agaattcacg caacagacgc agattataca atgtcaggag taatagccat ctcacttctt
    85561 tcggcaggga tgttttctgt gattagtgtt tttgggagat accgtaagag gggagtcctt
    85621 gatagattca aaattactcc tcttaggccc ttcacatttg tccttggcag cacccttact
    85681 agatttcttg taagtctggt ttccatagtt ctcatcttgg ttgtaagcag actattgttc
    85741 agaactactt ttcaaataga ctggcttccg tttctagtat cgattctgtc ctcgactctt
    85801 ggaatgatgg cgcttggtct gtttctcaca ttgttgttca gaaatccaga gactgccgat
    85861 gccgccgcga gtatattgat ggtgatcgtg gttttctttg cgggaatcta tttcccactc
    85921 tctttcttgc ccgactattt gagagtaata tccatattcc ttccagtaaa atatgtggcc
    85981 gagctgataa ggcattcact tggaattgaa tttgtgtcaa cttcatattt tgttattacc
    86041 aatcttgtgc tttttctaag tggtgttgta ctgttgtatt tcaccgggaa gcgttatctt
    86101 agcgcctctt aggtttgact agcgagtatc tgtgtcttct gtaggaagaa aataggaatg
    86161 aatacgtgat ttagagtaac agaagggagg ctttttgagt gagtgataca gtaagaattg
    86221 ctgaagggat ctactggata ggagttaacg attttgagac taacctcttc gaagcgctat
    86281 ggccgcttcc aagaggagta tcttacaact catacattct gatagacgac aaggttgtcc
    86341 ttatcgacac ggttaaaggt ccgttcttct ccgattttct cgacaagatc aggtcgcttc
    86401 ttcccgaagg aagaaaggtt gactatcttg ttgtcaacca tatggagcca gatcactctg
    86461 gatcgataaa agttctgagg gaagcctttc cagagataaa gataatcggg aacgaaaaga
    86521 cgaagggaat gttggatgct ttttacggag tgactgacaa cgttgtaacc gtgaaagatg
    86581 gtgatgagct tgagataggt tcaaagacac tccagtttca tatgactcct atggttcact
    86641 ggcccgagac aatgatgact tttgagaaga caactggact gcttttctcg ggagatgcat
    86701 ttggggggtt cggagccctc aacggaggaa tcttcgatga tgaagtcgat attgactact
    86761 ttgaggatga ggttcttaga tatttttcca acatcgttgg aagatacagt tcgatggtcc
    86821 agaaggcatt cgaaaagctt aaaaatgtag atataaaagt cgtctgcgca actcacggac
    86881 ctgtctggag gaagaatcct tcaagaatta tagagcttta cgatcgctgg agcaaagagg
    86941 aaaccgagga gggagtagtt cttgtttacg gttcgatgta cggaaacacc cagaagatga
    87001 tggaggcggt agcatccgga ttggtcaagg ggggcgtaga taagatcagg gtccacaata
    87061 tctctaccac acatatatcc ttcataatta gagatatctg gagatttaga ggtcttgtat
    87121 tgggaagctg cacatacaat atggagctct tccctccgat gaaacaactg gttagtgcga
    87181 tagagaaccg gatgatgaag aacagaaaga tcggcgtatt tggatcttat acatggagcg
    87241 gaggagctct caaggagctt caagagttcg cttccaaatc aaaatgggat caggtcggcc
    87301 cggtgattga ggtgaagagc tgtccgacac aggaggatct ggagaactgt gtcgaacttg
    87361 gtttgaacat ggcttccgca ataaagaaat agatcgagtg gatttgcaat tatcagttaa
    87421 ccggctcaga atatctcacg gtcaatctca ttttgctaca caagacgatc ctttttagtg
    87481 aagaatattc atcgaagtcg acgataacat tcagtttgga gcctcctgaa ttgcagaggc
    87541 tctttctttt cttctgagct gctactgcca tttgtcgcgt ttttgaccca aaccgagata
    87601 ttttcccttc gatcttcaca tttattgctc tgaagttctt tggttataga tcacaattga
    87661 agccggacgg tcgaaaatct agatttaaga agtcgaatct tttgaggaga atttctcgga
    87721 tcttctttta atctctatcc ctggaaacaa tggcaatacc ggtcgggctc atttggcatt
    87781 caaatctcca gatgatgtat tacttactga ctcaaatgaa ctcgcaattt cctaatagaa
    87841 agaattcagc agtagaacta ctacggataa ttatcccgtt gtgagcttct actcaaagat
    87901 gaataatgat agccacgaag ttagctgtct gtttaatctc tctttaattg gatgcgggtt
    87961 agactaagat gatcgataaa gtagagggag gttgcgaatt atgaaaaagc tttcattgtc
    88021 catcatacta ttgattacac ttttctttgc cgggtgcatg gagttccctt cacaggaacc
    88081 tgacagaggt acagtaagag tttttcttac cgatgcggta attcccgtag aggacatcga
    88141 ttccttgatg gtgaggatcg atagtatctc gctttacgga gatgaacatc aaccggtcgt
    88201 tatttcagat acacctttcg aagtggatat agtcactctg gtcggagcaa cgtacgaatt
    88261 gcccaataca atcagcgtct caggcatcta caaccagttg agaatggagg tcggagccgc
    88321 gaccttgact gccaatggtg agatcttccc tgttactgtc aactcagggt cgctaaagat
    88381 aaacgatctc tatttgacga ttgatgagaa cgcaactaca acaattgtgc ttgatttcga
    88441 cttatctaag tccctgatca taaatggacg ttgggaagat ctcaagattg aagaggacaa
    88501 tggaggtttc aattctcgca aagacaaggt ccatatgact cctgtcttac atgtgcgcca
    88561 cggtagcctt tttgacgtta ccggagttgc ctcttcaagc atttaccctc ttcttgtagc
    88621 gctctttcct gagagtggag gcgaggcact tacaacgttt acccatgagg acaatccaat
    88681 ctgggatcag ggagaattta gattctgcaa agtcgtttcg ggagattaca ggatagagtt
    88741 cttcgacaat tacacagatg aaggtttttc gattgacgag agcgaatcca gatatcagcc
    88801 tttgtttgtg acagttgtgg acagagacgt agatcttggt acaattgttc ttgtggagaa
    88861 ataatttctg tcgacgctgc tcttgagatg acctaaaagc ccttcttctt cactagcgtg
    88921 gcaaggtgca acgcgcagag tttctcgaaa aagatgagca catggaggtt acatgaagaa
    88981 aatcgtctgg atcttgataa taatgtctct ctttttctcc tccaagattc tttctaacgt
    89041 attaattgag ggcgagagat ctttgctgga ttatctagtc gaggggaagg ccctgaatat
    89101 ggcggttgat atctctctga ttcagagtgt ccaagaaaag gttccaggag agattcaagc
    89161 ggtttttctt ctacccgaag ggtcggtaag agtcattacc gattcccatt ttgtcacgct
    89221 ttcggaaacc ggtgagattt atgagattta tgagctcact ggatcgggta ccactttgaa
    89281 agttggaatc atagatgcgc tttctctcgt atctgttcag acaagagaga atgtatttgg
    89341 catcttcctt gtgcaggaag agaattctta cttctggcat gttgtgaccg ggactaggac
    89401 cttcaggctc gcaagcagtt ttgcctctac tcttagtata aagccaaatg aggaagcact
    89461 ggaagagata aacaaggggt tgcagaaatc tgaggaggta agggaaaatc agcatccttc
    89521 cgaaaagcaa ggtaaaccag aagatcccgg aaaaccggaa gatcctggaa agcctgatga
    89581 tccaggtccg ccaaatacac ctccgggaaa agaaaacgga aagaaatgat gtcctatgaa
    89641 gttactggta gttgaagatg agaagaagct tcttgagaat gtggctgctt tcctagaacg
    89701 caaaggattc cttgtagata ctgcgcagga tgcagaagat gggctggatc tagcgcttga
    89761 gagaaactat gattgcataa ttcttgatgt gatcttaccc ggaatgggcg gatatgaatt
    89821 ctgccgcttc ctcagagaag agatcggctc cgacgtacca ataatcatca tcactgccct
    89881 tggggaaatt gacagtagga tcgaaggtct caagatagga gcggatgatt acttgccgaa
    89941 accattcgac ctgagagagc ttgaggcaag agtcagagcg cttatcaggc ggaaccaact
    90001 tagaagtgag cctgttattc gatatgggcc gttgacatac gattcaagga gcggtgtttt
    90061 tagttgttcg aagaaggaac tgtcgcttac ttccagagaa tcctcgatcc tagagctttt
    90121 tttaaggaat cccggaacgg tctttagcag agaggaaatt acagacaaga tctgggaaac
    90181 tggattcgag tctcggagca atattgtcga cgtttacatt cgatacatca gaaggaagct
    90241 agaggctgca ggaattctag atattataca gacggtaccg ggaaaggggt atgggttgag
    90301 gagaatgcgg gatgacggtt aagtcgaaaa tcactctact gtacctcttt acttatggta
    90361 gtatagtcgt ttcgcttgga ctgaccgttt tcttcgttct taaaaatctc gagatgcgca
    90421 gaatagataa tctcttaatt tccttccaca atgacattgt caatacttac aaatacgcag
    90481 gcgaggagaa cagactgttg actcttgccg gggatgaaga tctggggttt gccatttaca
    90541 ctgatgaagt tccgattgcc agtttccgtg ccgaaccgga gtttttcaag gcagtaaccg
    90601 gaagcggaac taaaggagat taccgattta gatcttcatt tgagataatt gaaggaaagg
    90661 aagaaaagtt cgtgactttc tacgatttgg atgagtctca gaaatatctc agatttgttt
    90721 ttgtgacgat cgtaatcttc tctcttctga cccttctgat tgtcagcgta gtcggcatgg
    90781 tgttcaccag aaaactaata aacccttttg aagaggcagg gaatcagctt gagcgaatca
    90841 gcaggaatgg tctgaagaac tctcgagtcg agcttaggaa gaccggcgaa gaagtttcaa
    90901 aacttgaacg tgagatcaac aaatcactga tcagaatcga acagttgata gaagaggcga
    90961 agcagatgtc atctaagatt gcccacgagt tgcgtacacc gctagcagta atgaaaacaa
    91021 atctacaact ctctcttacc aaggattttt ctgaagatac aatgcgcgaa gcattgaaat
    91081 cgactttggg agaagttgat aaactcatcc gattgtctga agattatctg cttctatccc
    91141 gcactgagag ttcactgccg atcgagaaaa atctcattaa tttctctcag ctcttgctcc
    91201 agacgactga aaagatttta atgattcatt ccgataaaga tctggaaatc gatattgctc
    91261 cagatatagt gattagaggt tccggctaca tgctggagca tgtgatcatc aatctgctcg
    91321 acaacgcctg cagatacaca accgacggat cagtagaggt caaactgatt tccgatgaac
    91381 atggttcctt tctctcgatt tcgaacatgg gaaaagctat cgatttcgtt caaaacgaag
    91441 aactcgagga ggccaagcaa attaaggggt acggactggg ccttcgggtg gtcaggtcaa
    91501 tcctcagggc gcatggtttg aagcttgatt acactcatct ggatggaaag aatatcttcg
    91561 tgattaattt tcccttcgaa gagtgaagat ttagcctaaa cttacatttt gattgtagca
    91621 ttctgtcgcg ccgtagtcgc ctatatggat ttcttagaaa tgctcatcga atggtgtagg
    91681 aggtagagaa taaagcttaa cgatttcaaa ctggaagtct attttggaaa gcatgagttt
    91741 agtgcgcctt atcttcttgc acagtcggac tgcgaagcca tgacaattaa agaattgctg
    91801 caaatggcag gggagagctc gtccgagttt ctcgatacat ggcttggata cactgaagtt
    91861 tcgggccatc cgatattgag aaaggagatc tcccgcctgt actcgactgt tggagaagag
    91921 gatgttcttg ttcattccgg ggcggaagag gcgatcttca acttcatgaa tgttttcctt
    91981 cagaaaggcg accatgtgat ctgccagttt ccagtttatc agtcactgta cgaagttgcc
    92041 aacgcaatcg gatgtgaagt gacccgctgg gaactgagaa gaattgggga agaatggagt
    92101 tttgatgtag atgaactgaa ggatcttgtt cgcccagaaa ctaaactggt tgtgataaac
    92161 aatcctaata acccgaccgg ataccttgtg cctaacagag acctgatgaa gatcgcttcc
    92221 tttaccgaag aaagggggat tttcgttttt tccgacgagg tctacagagg gcttgagctt
    92281 gatcaatctg tagaaaagca gaagtccttt gcagacattt ccgacaactc tcttgcactg
    92341 ggtgtgatgt caaaatctta cggattggcc ggcttgagaa tcggctgggt tgtatctcac
    92401 aacaaaagcg tcctggaatc aatgacaaga ttcaaacact acactacgat ctgcaacagc
    92461 gcgccctcgg agtatcttgc ctggaaggcg ctgtgtgtag gagacaagat cctgaaaagg
    92521 aacagagcga tagtcgtcca gaactgggat cgggctgcgc ccttctttga gaagcactcg
    92581 tcggtattca gagtttcgag gccaaaggca gggcccatag cttttgtaca gttcgttgga
    92641 ggggacgtcg aagacttctg ctcggtggtc ctggaaagcg cgggaattct acttcttccc
    92701 ggatcgatgt acgatttcga aggcggcttc ttcagaatgg gattcggccg aaagagcttt
    92761 gctgaaggat tggaaaggct agacgagttt ctggggttgt ttggaaaatg attattcatt
    92821 taggcgacta ctgttgtcta tatgtgaata atagattcag atcgaccagc ctaaaggaaa
    92881 tgcagcttcc cgaattagat tccttaacct gcaggcaaca gccagaccgg ttgactttaa
    92941 caattactcg tggtaaaata ggtattgaag acgctttggg agcgtctttt ttctttcccc
    93001 tatgattcta ggtgaaactc tgatagccga acggagcgag agactcttag tgtcgtccag
    93061 ccgttcttgc gcttggaacc gatgtcccta tgctcctctc ggagaacggt gaaccgagaa
    93121 ctgattaccc gttcttccaa ctttgcaact tggatcttgg aactgacaac tgttctaata
    93181 gccgtggttg gttggagctt gaggaagaga agacagcaat cacccggcga agaatgtcaa
    93241 cttgtttgaa ctaactcaat ttgccatcct gatgtgctct tgatcaggat ctggtctttg
    93301 atcttatcgg tgaacgggtc cttgatctgg gactgtcaac ggtgagtcgc ttctaacagc
    93361 gcacagcggc tctcaatacc gagatgctga atcaagttca gcatgacgaa tagggacgct
    93421 tccagaattt cttaatactg tcatcccgtg atgcctttgc acgggatctc gccccagaat
    93481 ccctctcttg ttttgaatgt cctggtgtga aagagcctct ttcccaagat tcactcttca
    93541 cgttatttgg tcagtaatcc atatgtatgt tccttccagc aaccaagaac gggcatcgaa
    93601 caacctttct tgtggaacta tttcagatcg tcgccttctt tcgcaaacaa accactaaca
    93661 ccctccgacg ctacgtggcc cccgttccca atggaggact caagatcagg cttatcagga
    93721 tttaattaca gatttacgca agaaccaccg aagtagcggc atttaactca cagtcttatg
    93781 ggtgcaatga gcttgctaaa cgaagatgat ttggaagaga atgagagatg aacctgagcg
    93841 aatgagactg ggtcactttc tttggataga agcgaaatct tcaagacaac ggaaatgaaa
    93901 ggaagacagc gctatatcca ttcaatgctc aatgttgaag cacttcgaac ttacttcgaa
    93961 ttcactcaaa caactggaaa caggctcata atattcgcaa actctataac cctttcagtg
    94021 taatgagccc ttcagattgg tttgtccaaa tgatatcgaa cttggtcaga agtggttaac
    94081 ttgatataat attaattaaa tacaacaatt tgtatgtctg tgagatgcta agtgtcgtga
    94141 aacgattctc ccgaaatagt cttgggtagc gcatggcgag gtgcaattcc acctgtgaaa
    94201 taagatggta agttgttaag agtgtcatct gactgtccat tgtggcaatt gcccatttca
    94261 gttaggtcaa accaaggagg tgggagcatg agttcagaag aaggctctct gtttcatact
    94321 cgaggaattg acttgattga gacctgcaaa ggtggaaaag aaggagatat tctcgagtgg
    94381 tctgtgagac aacaacccgg ggggcagaga gatggagaat gaaaaaaaga cagttcgcag
    94441 atttgttttc aagctgttca aggtttactg gaaacgggaa ctgctggctg cagttctggt
    94501 cggcgtgatg attgcacttg ctgttgtctt tccgctcttg ataaggctgc taatagatgt
    94561 agttatccct tctggcgaca tggaccttct cgtaaagtat gttttgcttg tcgtcgggct
    94621 ctggtttgga agcttgatct tcacatattt tggagaggtg gtttttgaaa ccacggccct
    94681 catggcaaaa tcggacatgc gaaaacaatt gttagagaag atgtttgttc ttcctttcga
    94741 ctttttccac aagaataaga ccggcgagct cgtatcgaga ttgatatctg atctggaact
    94801 tgtaggaact gttatagcgc aggtgttccc gatactgtta ttgggagtcg ttcaaataac
    94861 ggcgattcta gcaatcatgt ttgcttttaa ctggaaattg actcttttgc ccctaggctt
    94921 catcgtgttt tcctttcttg tgataatgtt cttcaatatt atggtggaga aaaggagcaa
    94981 ggtggagagg gaaaaattcg gcgaactggc gggagtcaca agtaacataa tcgataatat
    95041 gaaactgatc agaatcgcca tgccattttc ctgggtcaca aatttcttcg atcgttttca
    95101 aggagaccat gtttatgcgg gcaagaggtt cataaaggcg ataaaaatcg ccggtgcact
    95161 caagacgtcg ataaacgggc ttatttcctt ttcactgctt gcctacggtg gttacctggt
    95221 aatgatgggt gagattaccg taggaattct gatgactttc tgggcttatg ttcaatcatt
    95281 gttcaaccct attcagctgc tcatgcaggt gaacattctt ctaagacaaa gctggggagg
    95341 actgctgaga acaatcgagg tgctggaagc tcctgaagag gaaaggagcg atagtagtcc
    95401 taagttagaa agtgttcgga gcatttcttt agagaacatt gcatttggct acggcaaaaa
    95461 gcaaattcta aaaggcctgt ctcttgaatt gaggaaaggg gaaattacga cgctggtagg
    95521 tgagagcggc gccggtaaaa ccactacttt gaatattctt atgggacttc aaaaacccca
    95581 gtcgggaacc atttacataa atggcgatcc ggtatcgaat tctgcattac tcagacccta
    95641 tatcggcttt gtggagcaga caccaacttt atttgaaaaa tgtactctcc tggaaaacat
    95701 taccctgggt agatcgattt ctaaagccag tttggaaaac atcttcgaaa gaacaaacct
    95761 ggatgtattg ctgagaagat tcgagaatgg actcgacaca ttagttgcag acattcagct
    95821 ttctggcggt gagagacagt tggtcgcgct ggccagggcg ctggtctcga atccacgact
    95881 gctgattctt gatgagatca cggcaaatat cgattcaaag actgaagaaa taattcagaa
    95941 tactcttttg ggtctgagat ctgaaggcat aatcgtgctg atggttgctc atagattatc
    96001 aacggtccta ctgagcgaca gagtggcgct aatcagtaat ggggttattg ttgcaaatgg
    96061 aactcacaac gagctgttga agaccagttc cgaatacaaa aagctttact cgcttcagct
    96121 gataaaagat gaatagaagg ataattggtc aagcaccgct aggtgctttg tgaaagcccc
    96181 ggactgaaag atatcaggca ggcttcatgg tgcgcgtttc aaatcagaca agatcgggat
    96241 gcgggtgtag tgcttggctc atgatgccgc aggggaaagg cataaataat gactttaacc
    96301 ccccggactc ctcttcctga agtcgtcgaa ggcaccatga aggcaaggaa aaatctcgga
    96361 acagagtcaa gggagcaaga ttgtttcacg tttgccccca acaacctatc ctggcggcgt
    96421 ttttccacat tacgacctga tctatcaagg acgcaccctt aagtatattt cgagaatgct
    96481 cttcgtgcta attgcagctt cagacccgcg attttccagt tcgaagagaa cgaagacggg
    96541 ccatttagcc cgtctacact agaaatcaaa attgtatctt cctgccagat gaaagtgagg
    96601 aaagaacgtc gagtgtcttg ccagctgatc tccaaggttg aatcccacat ttatgattga
    96661 agttgccagc tcgagactga agtcgctcat gtcgaagcct gctccgatgt gggagactag
    96721 cggaagatac tttatgtctc gagctgcaag gatgctagac agttcagtca tcgccgttac
    96781 cttgaagaag acaggcggaa gagaagacgc gagatcttcg agtcccagtt cggcataaag
    96841 tcctatgagt gattgcgtgg ggtcaagaga atagaattcc atttcctcaa agccgaatcc
    96901 gaagaccgga acatctgcaa taatgcctaa gacgggtttg ctaaacaaca tccagcctac
    96961 ctgagtcctc ccgaaaaact ggagatcaaa ttccttcgtc tgtctgtttt ggttgagaac
    97021 aaaccccata ccaacggaga aatgagtggc aaccagtgcc gaggccattc ccagaatcaa
    97081 aacaaatact gcaattcgtt tcactatcac acctcctgta ggttcaaatc aatatctcag
    97141 ccttgcggca atttcgcaat caagtagttc cttatctacc accactactt tgccgtcgtt
    97201 gtttacgaca ctccttacga tccacggacc tacgtttcta acctctctgc tgtctttgac
    97261 tgcatgtgta tatacgagct ctctcccaag cacgtaaccc ctctttctct cagttgggtt
    97321 aatgaaaagg gtatcgatcc ttctatcatt tgctgcttta gagacttctg tccatccttc
    97381 aacgtatttc tcgaaattct ctttcgcgtt gtgaagaaat tcccttcgga tctttctgct
    97441 cacgctctct ataagcttcg aggcatcttc ctgtagctgc ctctcttgat taagggtttt
    97501 ctggacgaag acagggtgtg cataaggcaa taattgcctc gcttcgtttt tgaacttctc
    97561 catagtctcg ttaaaggaag aagagtaata gatgactaga tagttaacat cttttggaag
    97621 atcttcgtcg ttctttatca gtgaggcaat cttctgaaga aatcttcggt tctcttcttc
    97681 ttttgccctg ccgactttct ccgaagcgtt gccgtgatac atcgctcctg aggaaggtga
    97741 gggagagtat tcctctatat actcctgttc ctttttccta atgaattcat tttcaatact
    97801 ctttattaac gaaagcttgt tgccatccat cgcgtagaaa gatgacttct ctctctccaa
    97861 cgatacgatc agggcgtcta tggccgaact tgaaagccag atcaactgat caagatcgaa
    97921 agtcttgcct atgtatatct ctttctccgg tacctgtgaa agagggatga tgctcaagcc
    97981 ttctatcacc tcatcggctt ttacgtcctc tcccatgcgg aattcaacga aaatcgccaa
    98041 tcctcttcga agttcttttg tatttcccaa accagtaaga acggcttcaa caattctttg
    98101 attgatcttt cccattcccg ataacttctc gtcctttctc aaatgctcaa ggatgaatga
    98161 cttgacatgt ctttcggcat cggctcttgt catgttttcc atagggaagt agaatgtgag
    98221 aaggtaagag tctgatctta tttccgcctc tacattcagg agcttttcga gttctttttc
    98281 catctcagct cttcgaagaa cgcgtggctt gaaattatcc atctaatctg cctccttcca
    98341 tgctttctgg tggtttgaaa gaaccttagg gctctactgt ctagaatgca tttgatctga
    98401 tcttggaaca tccattcgtt cacattggct gcgaccgttt caactcaatt ataaccccag
    98461 aaaatgtagt acaatagtcg cggagatgat tcgtttggct aacagtttac tatgcttgct
    98521 ccactctaga atggggagag cctttccggc cttaaaagtt aaaaatttca gatacttctg
    98581 gatcggtcaa ctcatatctg ttatgggaac atggatgcag acagctgctc agtcatggct
    98641 tgttctgaca attaccgatt cgccgttttt gctgggactc ctcggagttg ctcagtttac
    98701 tcctgtgctt ctattttcgc tacctgcagg agtcttcgtc gaccgatttc ccaaaagaag
    98761 tatcgttctg ctgactcaga cgatctccat gattctggcc ttgattcttg caattcttgt
    98821 attcacagat tcggttcagt actggcatat atttcttctc gctcttggcc tagggatggt
    98881 aaagacactt gatattcctg caagacagtc tttcatgatt gaacttgtgg gacgtgatgt
    98941 tgtgctgaac gcagtcgcgt tgaactcaac agtaatgaat ctgggaagag tagtgggccc
    99001 tgcggttgcc ggggtcgtta tggctctggt tggagcgggc tggtgttttc tggttaacgg
    99061 tgtgactttc ctggctgtga tctttggatt attgaagata gacgtcgaac ctgtaataaa
    99121 gaagcgacca aagggaaccg taataccgga tatcaaggaa gggctagttt acctgtttag
    99181 taagccgata ctgactacga caaccctcat tctggctatt gtgagtacct ttggcatgaa
    99241 ttacagcgtt ctaatccctg ttttcgcgag aaatcagctt ggtcttgacg cgcaaggata
    99301 tggccttttg atgtccgctc tgggagtagg atccatgctt ggtgcgctaa ttgttgcaag
    99361 cagcagttca agagggccga acagagttcg actctttgtc gttccgttca tcacatcatc
    99421 gctttttatg attatggcat tcaacagatc ctatgccttt tcggctttgg tgattggctt
    99481 gatgggattt gcgaatatct tctttacaac aactgctaac agtcttatgc agataaattc
    99541 cgacgatgag tttaggggaa gagttatgag cgtatattcg ctcgtcttcg cagggtcgac
    99601 gcctatcggg aacatgtttg tgggttcgat tgccgacaag gccggtggag gcagcgcttt
    99661 cttctgggcc ggcgttataa ctttttttct tgtggcggcc acgattatcg ctcacaaaat
    99721 aagaaagaga tcacttaggg tgaaaccgga ggaacatcct gactgaagaa tcaagaagaa
    99781 atgttgtata atctcaatgt ttatatccgg ggtgataaca tgtcgagtat tcccttctct
    99841 cggatttcca ggttctgggg gctaaagagg aaaatcgtac ttgaaaaaaa gactaacgct
    99901 agagctttca ttgtgaggtc tggaagcgtt ttttacgtat tgaagaggag gatcgcatct
    99961 cttccttcta gatcttgcca gactgagctt cttaaatacc ttgtccagag tgggcttccc
   100021 gtatctgcgc ctattccctc gggaagtggt gagttcgagg tgaagatagg aaaatatgta
   100081 tattcgctgt ttccatacat agaaggtgaa aaggccgatt ccaacatttc caatgccacg
   100141 cccgaaatgg tggaggagct tggaatcttc accggaaggc ttcacaaagt tcttaaagaa
   100201 gcagaaggaa gtttctttga aagaactaat ctgctgaaat ccctcacctc tgcaattagg
   100261 tatttcaact cgaccggatc tttcttttca agatcagata tggaaatgat agaagagttt
   100321 ttcacagatt tctacaagct ctattacaag cttccggtcc agataatcca tggcgactat
   100381 catccaggca acataatcgt acacgaagga aaggtttctg ggataataga ctttgattgc
   100441 gcgaccagag aagtgaaagc gctggatctc gcttatctta ttcacagtgt ctttgcagag
   100501 ttttcaagaa tgggttcacc ttcactcttc ctctacctgc ttcctcactt gcttgaaggc
   100561 tattgccttg agaacactct ttcttttgac gaacgggaga gcttgggtta tcttcttgct
   100621 gctatctcaa taattcagat acactatttt tcggcaaacg gactttctga cgaagcgaat
   100681 tttgcatcga cggttttcag atggatttac ctgaacaacc atcgaataaa ggaaaagtcc
   100741 ggactggttc taacgcgttc cgaaaaaaca gtctgaagat ttatcggata gtgaaagccc
   100801 ctccatgttt tcttgtctct cttgttctta cccgtttaac aattgcctcc atatatgtca
   100861 tcccttctgc attgtcttat cggataattc gttgattgta acgaagaatc tgcgatcaaa
   100921 tcgacgatag ccgtaattcc tcctaaagag aaaccgcata agaagaaatg gaacgatcca
   100981 ttcactccaa gtgtcttgaa agcgatctcc ctaggattcc caataataca agcattcatt
   101041 gacggattca ttctggttcg ctccaattat tcttaactgg catggtgcta tcgaagttat
   101101 ggcatataat ccgtttatag gaggcggact aaatgattgg gttgaaaatt gtgatcgaca
   101161 ggaactccga agtccctttt ttcaagcaat tagttgatca actgagaacg gcagtaataa
   101221 gaagagagat acctgagggg acacgactcc cttccgaacg ggagctttcg aagctgctga
   101281 gactgaacag aagtgttgtt gtgaaggctt acaatgaact gaagctggac ggacttctgg
   101341 tttcgaaaac gggcagtggc acatacgtaa tttcggccca ttcgcaaaaa aaggaattcg
   101401 acatgatttc atggggagaa caactcaact catgggctac ccttccggcc ggtccggcaa
   101461 gatgggtaaa ccttctcaaa aaactgtcgg atagttgcat acgtctagat acaggtgtgc
   101521 ctcacgtagg cgccaatcag gttgatctca tgaaggagat acttggaaac ttaaccgatg
   101581 aggaactgat cactacactg aattatcctt cgctaagggg ccacccggaa ctcatcaagt
   101641 atctttgcct gagaatgaac agctctggac ttcacataaa ctcgaggaac atgctgatca
   101701 ctgtgggcac agaagaagct atctatttgt tattctcggc tcttgcaaga ccgggagaga
   101761 cgatagtcat cgaaaacccg acctacgtta acgtaatcaa catctcgaga atgtttcagc
   101821 acagagttat ccctgtagag aggaactcgg agggtttgaa tctttctaca ctggagaacg
   101881 tgctaaatag aagccgggta aaattcatat acactatgag ctgctcgcac aacccgacag
   101941 gggcaaacat gcccgaaagg aaaaaggaag ccctcgtcaa actctcagag cagtattcgg
   102001 ttcccatagt cgacgatact gtgttttccg aaatccagta cgatcttcct gcacccaaat
   102061 cactcaagta ctacgacacc cataactgtg ttatcgagat cggtggcatt tcaaaggcct
   102121 tcgcaccagg gctcaggata ggctggataa tcgccgatga agcattgatc gaaaggctga
   102181 tcgagcccgt gaggatgatc tcacttggag tcccgaattt cagccagctg gttgcagcca
   102241 agatgctttc aacaggagag tatgacgctt tcctgaaaag atatctacaa ttgtgcctca
   102301 agaaaaggga gactacgaaa agagcttgtt acagacatct tcctggttac ataagagtga
   102361 acgaagctca ggggggcaac tttttctggc tagaactgcc gggttttatg gacggctgga
   102421 agcttgcgga agaaggaatt gaacagggtg ttgccgtcgc tcccgggagt ctcttcacgt
   102481 tcgattcgag tggcaggagt ttcgtgcgcc tcaatcctct ggggattccg catgatcaaa
   102541 tcgaagaggg aataagccgg ctttcgaaag caatcgagtc tatttcaaag agagacaatc
   102601 gtgaggaagg gacggtttca gtggtagttt agagttcgag actcaggaac catgtggatg
   102661 ggagaaatac ctgtgtgccc agaagagaag tagcgaagag ctcccggtca tcagtctcac
   102721 gatggaatgc tcgtaattga attcggttat tcttttcaag aaattcgaga ggcctctaat
   102781 cttgggaaga agataacttc tctctcctat ccgcaattct ctgctgttca tggagacttt
   102841 tcctgcataa ctggttttgg cgccactgcc aatttccatc tcagtaacag tcccctcgcc
   102901 cacttcgatt cctccgttta gggcgatatg ttcagcgaga atttctcgct ggatcccgat
   102961 gatctctgga actgtccttc ccgagacaag tccataatcg tctacagtca aggatgttcc
   103021 gcatttatca cagatcgctg aatctcctga ggctttgatc gtctgaaatc caccacactt
   103081 ttcgcagaac cagatgatct tctctatccc ttctgctttg ttcctgccct tgaacataac
   103141 cttcttctct tcctgccatc tccagtcaga agcttcagaa aggaaatcta gcgcttcttt
   103201 cccgttaaag gccttaaact ccacttctat ccttccccat ctatgatgat ccgcccacct
   103261 tggctttgta aggtatgccc cactcattct agcagcgaca accggtacat tgagcttctc
   103321 caggaacctg tctgttccgg gcggaacctc gccaaactca ccatcccacg ttacagagcc
   103381 ctccggcatc aggccaagaa ctccaccgtt gctcacgact tcaatcatct ttctcatggc
   103441 gggaatatcg gggacaccct tccttttctg aatcgctccc gctgccctca aaatcggtcc
   103501 cgaaaaaggg ttgtcgaaat ttccagcggc cacggcccat actattggga agctcgtaag
   103561 ggcctcgatc aaaacagcgt cgataaagta agcatgattg ccaatcagaa taaacggagg
   103621 tctgggcaac ttttccatgc ctattccata gagattgtat ctcttaatga agggaaccct
   103681 gagaatcggt ctcagcgctc tccagattag cttgtctcca ggaagtcttc tcttcatcgg
   103741 atcacacacc tacttttctt ccggatcggc tttgatcgta tccgtattgc ggccaagaac
   103801 ccgaagggga agagcccgtc ttcagcgcgc ctagtctact cctaccacca atatcgttcg
   103861 aagtaacggt ataatacggt gcgccttcct cactcgctac aggtgaaccc aggctgtctt
   103921 ttcctatgta ttcggatgac tcaatcttcc caaaagagtt cagttggaag atctttgcct
   103981 cgccacgtac gatcacataa atattaccat tggaagaaga aattaggagt cgccactatt
   104041 tcatcttcga gctccgtgct ccatatcaaa gatccttcac tggatattgc cgagcactct
   104101 ccatcgaacg cacgggcgaa gatcaacccc ttatctccaa ctgcgggcta gttgcaatcg
   104161 cagactctac ggggttttct gctatctgaa ggttccactt cttggaaccg tctaccggat
   104221 caaaggcata aaaatgacca tcatggtatg tgctgtaaac ggttcagtct tcacctattg
   104281 ccggactgga aaagaaacca gaatatatag tttgagtctc atatctccag agaaactccc
   104341 cacccttaag tgcaaagatg tagtttgaat cactcccccc ggttgtcatt atgcaagaaa
   104401 agactgttcc atctatcccg actacagact tcgaccagat ctctcgtccg gtttcgaagt
   104461 tccagagttc ctttccccct gaagagatgc agtagagaac accgccattt gttccaaaga
   104521 acacgttgcc aaaggcatcg aaagaggggc ttcccctgac tgtggtggct ccggcttcct
   104581 ctgttcctcc aaatgaccca tgaagtacac cttcactcga aagtttcatt agtttcccga
   104641 agtagttcca atatgtaggt caccctcact gttaatggat gcgtcactgc tgaaccattc
   104701 cccgctgctt gactccaaag agaagacctt cctgtaggag tcatgaattg aagtgaagga
   104761 gaatattgtg tccgtagtgt tcacaaaaac ctctccattt ctaccgatta ctgggctgct
   104821 gaaccgaaaa gacctatcca cttcagatta aaaacagccc aaaacaagga agagcgagat
   104881 gatggggcag atcgcaattg ctttcaaaac aacacctcat tgcttgtaag catcattttg
   104941 cgaatgtctt ctatcaatca tagctcattc atctctcgaa acgaaggttc ttttgggtcc
   105001 ctcctagatt ccagtttcgc agtgcaaact tcaagttggc gatcgaatca tttttccgct
   105061 caaaaacagt cgtaatcacc cacacatctg aatccaggct gtgtgaacct tgtcacctat
   105121 atgagaaagg actttcttca agagctgggg aaagtgccac gaatcttcat cgtcggccta
   105181 accaaaggtt gcacccttta tttgttgagt tttttgagat acaatgggac taatggacag
   105241 gatcttcatt gttgtttatt tcatattgac tttcatactt attgtacttt caaagagacg
   105301 aagggtactg atcgccttta ccgcaggtat gatgcttgtg gccctcaagg tctccgagag
   105361 tatgagaatc gaaacgattt cccagtttgt ggacttcaac gccatatttc ttctaattgg
   105421 aatgatggtt attgttgctg taattaaatc gacgggattc tttcagtatg tggcagtcag
   105481 gactttgaag gcgacaaaag gtaacatcct tttgctatcg gtgttatttt ccgctcttat
   105541 agcggccttt tcaatggttt tggacaatgt gactacgatg atcatgttca tgcccatcat
   105601 tttctttgtc gcagatactg caggattcaa tccttttgga ttcacggctg ttatgattct
   105661 cgcttcaaat atcggggggt gtatgaccct tgttggcgat cctccaaaca ttatcatagg
   105721 taacgcatcg aagattccat ttttgacctt cacaagtctt gtgtttattc cgcttgtact
   105781 gacatatata gttctaatgc taatctcgcg attcaaaatt ctaaggggcc tctcatctat
   105841 ttcggacaag aaagatgaga ttcaaggact taagctggac ggagttataa ccaacagaaa
   105901 gttgatgtat gtcagtctgg gcacattgat agttgttgtc attggatttg cagttcatag
   105961 tattgtagat atcgaaatgt cactatttgc ggttttggga gctgcatttc tgcttctgta
   106021 taccggaaag gactttgaat cggtggcaaa tgaagtcgac tggaacgcca ttctcttctt
   106081 catcgggctc ttttcacttg catattctct tgaaagcacg ggaataacaa gtgaactttc
   106141 cgatcttgcg ttaggtcttt ccagcaaccc cgcagttctt tctatgctta tactctgggt
   106201 aagtggaatg atcgcaatgg tgactggtgc cattcccgtc gtcacgatat tcattccgat
   106261 tgttgctgaa ctctctgttc actatccgct tcaatatgat ctctggattg cacttgcttt
   106321 gggagcaaac ctcggtggga atggaacggt aaccggccat cttgcaaatg tgatgtgctt
   106381 tgagatggtc aacaaagagt acggaaatac tcacagtttt ctcgatttca tgaagatcgg
   106441 attcccctct tccgttatct ctctggcaat ctcgagtttg tttctgatta ttaggctcgt
   106501 tatcttcgga taactatgac tgctagataa ccattactgt ccttatcaat gtatcgatca
   106561 accaaaggag cagtgttacg aaggccagtg aaagagaagt ctttataatt ccttgcctgt
   106621 agaagtcatt agcgatgagc cctggaacaa tcgtgcctat tacggttgaa aacagctcga
   106681 ctggaagatt gggcatcaga aaggtatcca gagagagttt gacgaaaaca cctagaacaa
   106741 tgccaagagc catcctcttt ctgccgtaga gaatcagata tctctggaga agattcagga
   106801 tgagaaatgt aacaaggcca accgcccagg tgtagagaag tctcaacggt tgttccataa
   106861 aaatgaaaag atagactgga gtcacaagtc cacctgcaga caatcccgtc acccaccaga
   106921 atgcagtact tatagctatt ccaatcgaaa acaaagcagg actcattaaa tctcctccag
   106981 accgttcagc cagtttacca ggccacgaat attaccgaat ccaaacacaa gctcgcttcc
   107041 gtctaaaagc gaatccagtt gactcggatc atctagcttc tctacgttct tgctgtttaa
   107101 aatcctcctt ggaaggggtc ctttaactat tgttctatcg aaactcctga tcatcgtttc
   107161 gaaaattgca gccctatcgg gcctatcatc tctggaattg aagaacccga taatctcttt
   107221 ctctggaaag tcccgcatca ctttttccag aagaagagaa gtagagtgta gatcgtttgc
   107281 agcaaaggcg tttgcaagaa cggcatttcc cattttacct ccgagcttca agtaccggaa
   107341 agttcccaca tcgggcagaa cctctttcat tccctcgata gccagcgatc tgtcaagccc
   107401 gagaagctct gcagctttga ttgtgatcga cagattttcg tcatggggtt tgaatttgaa
   107461 cccggagagg tattctgttg atgtagcccc aacaatcaat tttgtttcga ttttctcgca
   107521 ggtcttccta actttttcat aggcttcggg cgacacactt cctacgatac aggcctttcc
   107581 cggcgcaatt gataaggaaa gaacactagc cgtttcttct tcagtgcttc ctatctcttc
   107641 gatgtgatct gggaacgtgt tagttattac cgtgatgtct gcatcgagta ttcttctgct
   107701 tgccagttgc atctcgggtt ttatggccat gcattcgaca acaagtgcgt cacatttgtc
   107761 gcgccgggat tttctgagcg cgagaaactg ctcctgtatg tttgcggggc catggcgaat
   107821 tatctctacg tgtttcccct ctggagtaat ccacagaggc acagtccctg tggtctttcc
   107881 aagaacccta aacccgttgg cttttaacgc gccatggatt aaccgcacgg tttcactctt
   107941 ccctctagtt ccgtttacct gaacgacgac cttgagccgc gacctgcgct ttctagatat
   108001 tacgttttca gccaacaggg ctgaaagaag aatcaaaagt gcagttcctg caagaattat
   108061 gttgatcatt tatgaatcac ccgtcccaca acgatacaga gaaccatcag gcacagaaag
   108121 acgattatta gaggcaaaga gaaactatca acaaaaaaga cgccgctttc tcctggttcg
   108181 ggaaatggga ctggatcaat cggaattccg ttatccagag caagatctct gatatttagg
   108241 agatggatca ccggtactcc gagttcggca aactcaaaga tcagtcctct ttcatcgcca
   108301 tcaggtatcg aactgaacgc cgtaagcaaa ccgttatcga gacttgtcgc aataagtgaa
   108361 ttgccgaaat tcggcgctgc accgcccacg tttacgaaaa cagaaggaag cccatcggcc
   108421 tcctggatat agatctccag acgtttgtct atgctttctt gaagagattc ttcgtagatg
   108481 aaagtcttgc cggaagacag agcgatattg gtcattagtt cgcgcgcgcc cggaaaaaac
   108541 atcccgcttg ctgtatcgta gttgcctcca agtgaaacgg caatgagact atctctgagc
   108601 aggccgactt ctacgaggcg attcagcata tggacgaatg tgaactccgg gtgagttgca
   108661 ccgtgttcag aggcacctat cgagtaaatc acaaacggtt ggagctccaa tgtctcacag
   108721 gcgcaaagcg tcgcaagtag aagagacgga aaggagccgc ttgcaccgat agctaccgga
   108781 tcacctttct caagattcag ttgtttgaag aacttaacca taagggccgc aaaatccggg
   108841 ttcgttgaag ttctcttagc ctgaagattg ccgagagtag ttgtaatttc ggtgaattca
   108901 ggacctataa ggcctgtcaa gttagggtcg actgagggat cgatttctat accaagtgat
   108961 tcacgaaatc tggaaagttc ttctattgat gaagccatta ggcgagccgc ggcaacctga
   109021 tccgcgtaat acggggactc tatcctttgt cttgactgac caaccagaaa aaaaccgaac
   109081 accccgatca ttgcgccgag cagcaaccac ttcaaagaaa tgttggctcg gctgtaaggg
   109141 actgtgagta aaacatgaga actcttcaaa acaactcctc aaacatatcg acgaaaatgt
   109201 caacttcctg taagcactct gataatcgca agaaccaccc ctgtaatccc ttctcctcca
   109261 agaagaccag acgatatgac cagaccgttc tctttcgact tcggattgag ctttacggag
   109321 ataagactta tcatgcctcc cagaaaagcg gcagacgata tctccatggg aagatacatt
   109381 cctattccca gggtcatccc ggggagtcca atcagataaa tcagtattcc aatcataaga
   109441 ccgaagaaga aagccggagt attgggaaga ccaccgacca ttgtagaaac ggcatacgct
   109501 tgtggtgctg gaagctccgt tcctggaccc attgtaccat aggccctgaa cataataaac
   109561 aggacaataa ccgacacaac tgcaccgatt attccaccga ccgtctcggc tatgatctga
   109621 gctttgggat cagttttcaa gaggtagccg gacttgaaat cgttgagtac atctcccgcg
   109681 agtccgcaag ccactgcgac aactccggcg acgaggaagg cctcaatggt gccgatatct
   109741 gacactacct ttacccctaa gagaattatt attccgaaga cctccatcgg attgatacca
   109801 gactgcccgg taatcgaagc ggccattgcc gttgtaagcc aaactccaac aatcgtaagg
   109861 agagatggaa ttagcgtcat ttccgtcaat gttgtcagaa atacggcaac cacggcaaaa
   109921 aagataggag cgagtataag tttccttcct aaccttccct tttcacgctt ctgcgatctc
   109981 ccatatatct cctttgctcg agggaggatt ccctttataa gaattcccag tccagttcca
   110041 accattagcc ctattcctag gctgtctttg aacgcagcgg cactagcgac atcttcaaac
   110101 cagccaagtg cgactcctac ggggataacg aagaagtagc taagaaccgc tccgagaaac
   110161 caaacaccgg taaatagggg accgattata taaccaattc caactgccat gggcgagatc
   110221 cacgtgccga agaagatgtt tttcgaatag agccatgcag aagaccaggc agccgggatc
   110281 cagcccaacc cgtctcttat agcgacgaag actgcagtaa ttcccattgt ggagaagaga
   110341 taccttgctt tccttcctcc ctcgtcaccg gcaacgaccg tttcatatga tgccacaccc
   110401 atgggaaaag gcagcttctc tttctccacg aaatgctgtc ggataagcga agtgaatacg
   110461 acacccaaaa cagtgccgga taaggtgacg atcaacaacg aaagaaaccc cactgtcgac
   110521 gtctcctcaa ggatccagat tccgggaatc gtaaaagcca gaccaccagc taccattgct
   110581 cccgacgaca ttgcggtgtg cgtaacgttt atctcgttca gattggttct tccaagcgcc
   110641 ttcaatattg cgagggacat tactgcaacg aatatcgttg gccagggaag agcacccatc
   110701 ctcaaggcga catacatgga actggtcgtg attactaccg aaccaaatcc tccgattaca
   110761 aggcttctaa tagtcagctg ttttgaacta cccttttcca gcgacattcc accgtttttc
   110821 acgctctgtc cctcctgtaa atcgaatgag taacactcgg tcaagtcttc cgttcatatc
   110881 aagtctcttt acgatataga taacggctaa ccgcggttgc agtccagaac gatcagtcgg
   110941 atctattcca aagcaattta gccactcaaa tgcaatcctt ctcaagattt cgctctggaa
   111001 tttaagccaa gcgtttttgt gatcgctgtg atgttcgaga gatatgcgga atttcgaaaa
   111061 taaatctcct ccggaaatct cgtaactggc ttcatcccgg atgctgtcct ctactctagt
   111121 gaaaacgaat ccttcgatga gtttgtattt ttctcttgaa gttatccact gtatagcctc
   111181 aaatctctcc gatttatcgc cgcaaaacta gtcgtaatta aacataaagg tcttcgattc
   111241 gcatccacgc gtacttcaag aggtcttcaa ttatctcttc tctttcagct tcacagtttg
   111301 ccaacttgtc agggaaaaag tctccttcat agaagactgc ctttccggta cctgtatcgt
   111361 gatacgctaa catcgtactc acgacggtct atctccatca aattaacttg cgtatcgatg
   111421 tcgatcggca gtcccttcat tgttgtacac tcttttcagt agactcctgt ttttgtgcag
   111481 tttcttcgcc cgataccagt ttacaacatc ctggcctgag cacgaaaggt gcagttctaa
   111541 tctagaaatc ttctgaaaca tgtgaaccgg agttattttc gtaatctttt tgccagcttg
   111601 cctccagtga actgaagaag ctggctgatt gccacgagaa cgataacggc ataaagaact
   111661 acgtcccatt gaaacctgta gtaaccatag ttgattgcca gctgacccag cccaccagcg
   111721 cctacggccc ccgctattgc cgtgtaggtg acgagattga tcagtagtac ggttacgttt
   111781 ccaatttgat cgggcagcgt ttccggaatc aaaacccttg tgaccatctg ccatctattc
   111841 agtcccatcg agtttgatga atcgatgatt ccagaaggaa cgccgttgaa ggaattctcg
   111901 gaaagccttg ccatgaatgg gatagccgca atcgtcaaat tgaatattgc tgcgttgggc
   111961 ccgattatcg tgcccatgac tcgtttagta acgggaatca aaagcagaac aagaataatg
   112021 aacggaatcg atctaaaaat gttcaccaag agatctaaca aagcgtaaag ccctttaagc
   112081 ccgcgagatc tcgaacacag gtacaatccc accccgaggg gaatgccaag aaaagtagcg
   112141 agaaatcccg agagaagtag catgtaaaga gtttcaagag ttgcgtcaaa aatctgagat
   112201 agcatcagca tacctcctca aggaagaata cggattccct tagcttttcg acgaagagat
   112261 cctgtttctc gccctcgaca ccgataatta gagttccaaa gggaccgttc ttgaattctt
   112321 ctatctttcc atagaggata tttatcatca catcgttctc ttttgcgatt ttccagagaa
   112381 cgggttcgtg ggtccgtgta ccccagaaaa gaactctaag gatcttcttg ttagccgaaa
   112441 gtaaaccttt ctctagttcc aggggcttac cgaagtcact cacttcattg ttttctatct
   112501 tgacgaagaa ctcatgggtt ggtccgaaga agtccagctt tccattctgc aggtatgcaa
   112561 ctctgtcgca ggttcttttg ataacatcca tatcatgagt aacaacgacg aaagtgatgc
   112621 cgtgtttctc attgagactt acgacgagat cgagcagtct gcaagtcgtc tttggatcga
   112681 gagccgaagt gggctcatcg agaagaatca tctttggatc cataacgaga gcgcgagcga
   112741 ttgcggctct ctgctgttca cccccagata actgcgaagg ataggctctc agcctgtgtt
   112801 ccaggccaag ctctgtagct atattcatta ctctctcttc tacctctttc tttgagacac
   112861 cttgaatctt cagaggaaag gcgatgttct gatataaagt cctgtaccga tacagattga
   112921 agctctgaaa gactacgcca atcttctttc ttacatttcg cacttcgctc tctttcagtc
   112981 caacgatgtc aagaccgtcg aagaatatcg atccgctatc gacaggctgc aggaggttga
   113041 gggttcggag cagagaagtt ttccctgctc cggacaaccc tacgattccc acaatttctc
   113101 cctcttcgac cgacaaactc acatcatcga ggatctttct ctttcccgat ttgtcttcaa
   113161 aagatagatt tagcttttcg acccttagaa tcaaaacact gcgcctcaaa agaccggtac
   113221 tacggcacca tcgtactttt cattaatgaa gactctgacc ttttccgttg tcagggcgtc
   113281 aattagtgcc ttcagccacg cttcgtcttt gtcgacttca cgaatggcta ttatgtttgc
   113341 ataaggagat tcggctcctt caatgaattc cgcgtccgta attgcgttga ggttgatagt
   113401 aagggcgtag ttcgtgttga taacggctcc gacaaccgtt ttgtcgtctg tgtagactct
   113461 gggaatgaat ccagcctcga tctctacgaa agttagctgc tttggattct cttttatgtc
   113521 tagaactgtc gactccagtt tcgtaggatc tttgagctgt ataagtccgt tgttgtggag
   113581 aagaataagt gcgcggcctt cattagttgg atcatttgga agggcgatct tgtcgcccgg
   113641 agccagctcc gaaagaggtt tcttcaagaa gaaccccatc ggttcgacgt gaatagcctt
   113701 agcagaaacc agtcccttta ttcccctctg agaagtgaag gacacaaggt aaggaagatg
   113761 ctggaaataa ttggcatcaa gctcgccggt tgaaagggca atgttcggaa gcacgtagtc
   113821 gttgaacaca actatctcga gatcgaaccc tttttcagcg aggtcggctt ttatagcctc
   113881 aaggatctcc gcgtggggga cgggtgaagc acctatcctg atcttcgtcg taccgaacgc
   113941 aaacaccact cccagtagaa ccagtaacac aaacagtctc ttcattcaaa tccctcctga
   114001 atataaaaaa agggcgtctc tcttgagacg ccccgcttct attttctcag gtacagctaa
   114061 gcacccggag aaacaagggc gttgccgtac atcatcatag ctattctgtt ttttcctctg
   114121 ttgacattca tcttcataat tcaaacctcg tgaattgttt agctcattat agcctaatga
   114181 ctctttccaa agcaagtcgc taatgtaaaa tatagctcat tatctatctt gaatcaactg
   114241 gcctcgtcat aggtacaaat cttcaagtag cgctccccat tatccttatc cttataacag
   114301 gctttcaaaa gcctgcaact ggttgtcttc ggatccttgc atataaagaa agtgaatgag
   114361 agatccttgg cttttccctt cagcaactcc atcctcaatg ttgaaagaag actttccaga
   114421 cgtttctctt cgttctggcc atacattctt gcgttttcgt cagttttcac catttcattc
   114481 caaacgttct tggaaatcac aacgggcagc tttatcccca cttgctttgc aaaagatgta
   114541 acatctaccg aaccctcgtt cgaaaactta aaagaacgag aatctagtga tgcctctccg
   114601 atgctcatct atagcacctc cataataatg ttattactaa cataacatcc atgttggaga
   114661 ctcgcaaagc caaacagaag aacttgaagc tctctggaga tgtttaagcc ggtttatctg
   114721 ataatgcctc caaaaaccgt tgaacctgag ctcttattca ccgattattt ttatcagaac
   114781 tctctttcgc cttttgccat caagttcgta gtagaaaacc tgctcccatg gtccaaagtc
   114841 aagctttcct tcagttactg ctatgacaac ttctctcccc attaccgttc tcttcagatg
   114901 agcatctgca ttatcctcga atccgttgtg ctcgtactga gaatatggtt tttccggggc
   114961 gagtctttcg agccaccttt cgaaatcctg atggagtccc gactcattgt cgtttataaa
   115021 cacgcttgcc gtaatatgca tcgcatttac cagcacaagt ccctctcgga tcccgctttc
   115081 caaaagcgcg ccttccacat cgtctgtgat attgattatc tctcgtctca tctttgtgtt
   115141 gaagaaaagc tcctttctgt aagatttcaa ttctttcacc tcccgattgg ttcagaacag
   115201 atagggaatc aaacgccagg tctttttgca gtactctctg tattcttctc caaatataac
   115261 tttcagattc ctctcctcta cagagattct gtaaagaatt ccggttgttg gaaggacaaa
   115321 agcaaggatt gaactcaacg gatagttgaa gaccagagaa agccccagaa aacctacgat
   115381 ctcggctgca taggccggat gacgaatgac cttgtagaac ctgtcggttc tcagtttctg
   115441 atctggaagc gcgcccacat caattgcgaa ggcggggccc agttgtctca ctacgaagaa
   115501 acggataacg acggcgaaga tcatcattcc cactcctatc catctatgag gaaggaaagt
   115561 catgagaaag acctccttcg aacggccatc gactgcaatc gcaccgaaca tgcccgaaag
   115621 aacaaaggca agcatgatgt atttcgatcg cttctcctgg agtctctcgc tgatcttcct
   115681 gttgtgaagg aagaagagat agatatcgaa tccaacccag aaaaagagaa cgatgaagaa
   115741 aagcaactga ccagacataa ttggacctcc aggcgaaaac aatacttttc aattctatca
   115801 tcgggagtga cgaaggagag acatttttaa acctccgagg gaagtccatg tgcggttcat
   115861 cgattgtgag ttcggtaaga tgaaaaagat gtagtaaaat cgtaatatag gtggtgattc
   115921 gatgattttt ggaagagatg agagtgtact gctctttcga ttcgaagatg gtgaggactt
   115981 catagacaca ttcgttcgga cgctttccga taatgggctc cattcgctta tagtcgttag
   116041 cggaatcggt atgttgagag acttcacgat cggttggttc aactcggttt ctaaacaata
   116101 cgagaaggaa cttgtcacag ttcctcatga acttctccac atatcgggta atgtctctct
   116161 caagggaggt aaaccctttc cacatcttca tgtaacgctt tccggtccct caagaagtac
   116221 agttggaggt cacctattcg gtgggacagt atgcaacacg actgagctct tcgcaatcac
   116281 gaccggaaca ctgaagcttt tcagagatgc cggcagcgag acatcaaagc tggaattcag
   116341 aaagtgagct cgctgatttg agattccttc actgttcaga cattcatctg gggagaagac
   116401 ccatcggctc gcccaacagc ccctattctt atacaagata cgaagactac ttcgatgcct
   116461 ttgaatttgt ggttgattgg gcagtagctc acaacatcga tgcttttttc attaccggtg
   116521 atctatttga caagagagaa ataaatccag atacccttgc acggacacaa aatattctct
   116581 cccggcttaa agagagtgaa ataagggttc tggcgataga gggaaaccat gacaagagct
   116641 tcaacgttaa cgaatcctgg ctcagatttc ttcacgaaac ggcgtacctt gaactgcttt
   116701 ctccagttca aggggataac gatatactct ttccctacgt tgagatcagc gggttcaaag
   116761 tctatggtct tgggtacccg ggctttttgg cagaggaaat gatttcgaaa gtcgcgagta
   116821 tcttgagtgg aagaaacaac atagtgcttg tccacactgc gcccggtaac gatagcttta
   116881 ttccagggct agttacgccc gaagcactga atatccttcg atcgaaagtg atctatgcag
   116941 ggggagggca tcttcattcg cagctcgctt ttcctgcgga tgatcccttc ttctttgtcc
   117001 ccggatctct tgaatactgg gatgtctggg aaaacgatag aaaaggcttc tacgttttcg
   117061 atacggagaa agagtcggcc gatttccacg actcacatcg gagggaaagg caggagttct
   117121 cggttcaagt ggacgaaaac gaaagacttt cagatttcat agaaaaccat gaaattcatc
   117181 aaggtgcgat agttttggtg actgtttcgg gacgcggcga tttcaatgtt gatcagggga
   117241 gccttgaaag agagatagag tcttgcggcg cccttaaagc attcgtgaaa gtcaggagaa
   117301 ctgatcgtga aatcactctg gatgattaca gatcaatgag tgcgagagac atcgaaagag
   117361 aagtgataat gagttcaaag gagtggaagg agattggggc taagggagag gaactggtag
   117421 ccgcgatcga taaaatgaaa cactttcaag aagaagggag atacgatctc ttcaaagact
   117481 ctgtagattc cttcctggaa agaattatcg gtgatgtgcc ttgagaataa cttcgatcga
   117541 tctcgagaac ttccgaagtc attccaggta ttcagggacc ttcgaaaagg gaataaacct
   117601 gattcttgga agaaacggtt ccggtaagtc atctatcata gaagcgatag gactcgcgct
   117661 tttcggagga ggtttgcgcg acaggcagga ggacgccgtt agatggaatg aaagaagggc
   117721 aaggatcaag gtagcctttc ttgcagatga tggactcgaa tatgttgttg aaaaggtttt
   117781 tggtaatgca tcaaaccacg agctttatca gggagagctc ctggtagctc gaggaaggga
   117841 aaacgtcata gagaagataa ggaccatctg cggtcttcag ggtgacattt cgaggacctt
   117901 cgaaaatgtt atagtggctt tccagaacaa gatagccgac cagtttctcg gcagccccgc
   117961 agtcagaagg gactacttca acagggtctt ccaggttgat ctctacagaa aaatatcgac
   118021 ggacttcatg agaagctacg tgagcgattt aagaaacgaa gacgagtttc tccgaagaga
   118081 gattgccatc cttgacagtc aactggaaag aaaagaagaa gtcaaggaac ggatggcaca
   118141 gctcttgaag gacctttcgc ttacaaagaa ggaattggat acgctaagag atactcttac
   118201 aaagattaga agcgagaaaa ccggtcttga agaagtctcc agagaacttg cctctcgaaa
   118261 aagcagcctt gaaagagaac ttgtctctct gaaagacgct tcgggcaatc taaagaacaa
   118321 tctgaaacaa agagagagag ccaagagggc gaaggaaatc gtcgacgaat caagggaggc
   118381 ccatgatctg tatgaaaagc ttcttaagga ggagaacatc ctcaatactg aaaagaggag
   118441 attggaaggt ctggctgaag aatcttcgag aatcggcagg aagatctcag aatttgagat
   118501 cgagatcacc cgattgacgg agtcgctttc gaacaaaaac aacagactca gagagattaa
   118561 tgagcgtttt gaagcgcaga agatggaacg cagtgggcta attggagaga aagaagccgc
   118621 cgagctagag tcgggaaaga agagaatggc ttacgaactc agcgtttcga agaagaaagc
   118681 tttcaaagtg atctatgaac aatatagaga aaatgagaga cgcatcaatg cgcttgagga
   118741 tttatgggca agcgtccaaa atttggagag cgttgaggag ctaaacctaa ctctgcagaa
   118801 gatagatgaa gagatagaga aaacaaatca ggaagtcagg atcctggaaa agctgaacga
   118861 ggcaaaaacg acaaaatctg cgcaaaaaaa ggcactgaag gagtatgagg gttcccttgc
   118921 caaaggtatc tgcccgttgt tgaacgagag gtgccgtaac atcgaaaaca gagaagacta
   118981 tggaggatac atagcggagt taattaaagg acttgaagaa ggcgaaagag agcttttgaa
   119041 agagataaaa aaagttgagg atactccgtc taggctgaac gagctcctgg gggaaaagac
   119101 tctgacccta aaagcgattg aagaaagagc gaaaaaagag agtgagaagt cgaggattga
   119161 agaagagaag agcaaactga tgaaaactca taaaaaactc attctagagc tttcatcgat
   119221 ttcgggtgtc cctgccgaag tcgaacaaat gagtgaagaa atcgagaggg agattcgtga
   119281 agcttcgaca gaactcgaac ttcaaaataa gcaggtctct tcgcttgccg atcagataaa
   119341 gagaatcgac tctcagatcg aggaactgat tatcggcagg aatatactgg atgaagagaa
   119401 gaaggaaacc gaaaaagcca ttgcctccct aaggacggaa acagctaatt tgagaagctc
   119461 attagaagaa tatgcaaagg aactcgacaa acttccggaa ttcaacacac gaatcgaaga
   119521 aacgagaaga acaatggcct tgaacaaggc tggttatgag aactacaaca ggtttgcgac
   119581 cctagcaggc tatcttgaag agtacgcgtc gctttgtcaa agctcgcttg aaaagtgcag
   119641 aaatacttct ctttcgattt ctaagatcag aaaagagtat agctccctcc agagggcctt
   119701 cgattcagaa cgattcgagc agcttagaat tgaagagaag gctgtggaag agaaatacaa
   119761 tctgaaacga gatgagacag tatccatcgg tcgagatctc ctgtcggcaa aaaccgatct
   119821 taaaaacctt gccgacatcg aaaaacagaa gaaaacaagg cagaaaacga tagagaagat
   119881 aggcaaaaag gtcaaacttg cggaaaagat aagggagaat ctcaacctta cgggagagag
   119941 aatcacagcc ggctttcgcg atgcgatcga aaggaaggct actttggact acatgagaat
   120001 atccggtatg gaagagacaa taagatggaa cgaagactac gaggtatctg tcaaagcacc
   120061 tgaatatgaa ggtgggagca taagaagttt ttcaaatctc tcggggggtg aacaggttct
   120121 cgtctcactc tgtttgcggg cagctatgac tggcatcctg agcggagcaa gatttgccgt
   120181 tttcgacgag ccgacttcga atcttgacaa agagaggaaa gagcttcttg cccactctct
   120241 aagagaatta ttcggggagc tggatcaatc tataatagtt actcatgacg atgtcttttc
   120301 tgagatggca cagaaagtta taaggctcga acccgctgaa tgaattagag acacagtaaa
   120361 tcggaggtgc gttctttgaa gacaatactg aaaaacatcg actatctcgt gaccatgaac
   120421 gaaaaaagag acgaacttga agatgcattt gttgttgtcc aggacggtgt tataacagat
   120481 gtgggaactg ggaccccttc tcctggggat atagagatag acctttccgg cagaatcatt
   120541 ctgccgggct tcgtcaacac tcatcatcac ttttatcagt cactctttcg tagtgtgaag
   120601 gaagtcgcat cggcgaaatt gtttgactgg cttgtcttcc tttatgagcg gtggaagaac
   120661 ctcgataatg aagcgatata cgtgagcagc aaagtggcaa tttacgaact gatgatgtct
   120721 ggggttacaa caacaaccga catgttgtac cttcacccga aaggaagaag tgagttcatc
   120781 gacagcgaaa tagaggcggc gcgcagtact ggggtaaggt ttcaccccac gagaggctcg
   120841 atgtctctct caaagaaaga tggagggctt ccccctgact cggtcgtcca gagcgaaaga
   120901 gagataattg aagattcagt taggcttata gagaagtatc acgacagagg aaaaaacgct
   120961 atgctccgga tagcgctggc tccctgttct cctttctcag ttacagagga atcaatgatc
   121021 agaactgcag agctggccga gaaatttgat gttcttcttc atacgcatct ggcggagact
   121081 cgcgatgaag acgacttctg catacagaaa aaggggctga gacctgtcga attcatggat
   121141 aaggttggct ggttgaacag gcgcagctgg ttcgcacatc tagtatggct tagcgatgag
   121201 gacattagaa aactctctga aaacgactgt ggagtggcac actgtccttc atccaatatg
   121261 agactcgggt cgggaattgc ccgtgtgaaa gagatgaagg aagctggaat gagaataagt
   121321 cttgccgtcg acggcagctc ctcgaacgac acgggaaaca tgcttctcga aattagaaat
   121381 gctctgatgc ttcaaagggt tttgaaaggt gccgatgctc tcactcccag agatgttctt
   121441 gaatttgcga ctcttggagg ggcccgggta ttgagaatgg atgatttcat aggatcggtc
   121501 gaggttggaa aatcggcgga ccttgctgcc tttagacttg atactctgtc tatggcaggg
   121561 ggactctccg atcctgttgc atctctcgtt ctctgtgatc cgcacaaaag tgacatggtc
   121621 atggtaaacg ggaagatccg tgtccagaac gggagggtaa tcgatagtga acttccaggt
   121681 ttgataaaga gacacaacga gatatcttcg aagctgatcg gaggggagta ctgatgatag
   121741 ttacagtagt tgatattcaa gtcaggagcg agtttgtaga ttaattcata gaagccactc
   121801 gtgaaaatca caagaagtct gtaagtgagc cgggtaattt gagattcgac ttccttcagg
   121861 ataaagcaga tccatcccat ttttttctct atgaagccta tgaatccgag gaagcagctg
   121921 caaatcacaa gaggacagat cattacaagc aatggaagga aagagttgag gagtacatgg
   121981 agagaccacg caggggaact caaacaagag taatagaacc tgagaggctg agagactgga
   122041 aatgatctct gttgcagtct gagttcggga gggaggtttc aatgctgata gaagagttca
   122101 gaaggagaat tgatggtctt agggaagaga tatcttccag aggagccggt gcttttctga
   122161 taagcaagtg ctgcaatttt tcttgggtaa cttttggagc caggagtcat attactctaa
   122221 attctacgga aggcgaggca tcggttctgg taacacttga cagggtttac attatcacga
   122281 acaatattga aaagcaaagg atacaggaag tcgaatttca cgaggagctt ctgggtgagt
   122341 ttggatttct tgagtacaac tggtttgaga cttatggtga aaagaggtta atcgacaacc
   122401 tgctaaaagg aaagcttctc tccgataccg gaaggtatga cagcgaatac gttgacctaa
   122461 cagaaatgcg cacagtcctt agtgtttatg agatagaaac ctacagagct ctcggaagag
   122521 attgtgatga tatcttttct tcaattattc cgggtttgaa gaggcatatg accgagctcg
   122581 aagttcaagg tctattctat gaagccatgt caaagagaga tatcgaacca atactgacac
   122641 ttgtcttttc cgaggacagt tctctaagtt acaggcacaa cctatctcga aacgtcaaac
   122701 ttgataggag aggatttgta agcatctgtg ccagaaggcg cgggctaatt gtttcgtcca
   122761 gcagatcgtt catgttcgag cctgtggagg agatcagcct tcagcacgag cggaactgtt
   122821 ttgtggatgc tgttgcaatc gattcctcaa agccgggaga gaggctttcc aatgtctttg
   122881 agaagcttgt ggaggcctac gaacacgttg gcaggggagg ggaatggaag cttcatcatc
   122941 agggtggagt agctggctat ctgccgagag aagtgcatgg caatctcaag agcgatttta
   123001 ttctcaagag gggaaatgcc gtcgcatgga atcccacaat aaggggaact aagtccgaag
   123061 acacggtatt gattggagat tttggagaca gcaatatttc gttcccccag accagttcct
   123121 ggccagaact gaagtacgaa ttcgacggct cgacggtgag aagaccggcg atcctcctgg
   123181 ttgacaaggt ctgacttcca gtgtgacaac tgttttcaca attgacttga agtctttcaa
   123241 tttgaaacga gaaaagaggt tagaatctct tgggcatggt tttctcttga aaaacagagt
   123301 ggaccatata aagggatctt ctgaagatcc cttttttaaa attgtcgggc cctcatgcta
   123361 aaataaattc cgttcagcaa gaacggaggg gtacttatga agacggttgt tactaataag
   123421 aaggcgcgtt tccagtatca ccttatggat tcgtatgagg cgggaattga gctcgtagga
   123481 accgaagtga aatcgctgcg tctaggtgga gcatctctca ttgatgcgta ctgcaagatc
   123541 gaaagcggcg agatatttct ggttgactgc aatattagtc aatacactca cggcaatgtc
   123601 tggaatcatg aaccgcgcag aaagagaagg ttactgatgc acagaaagga aattcttagg
   123661 ctggaccaaa agacaaagga aaagggactt accattatcc ctttgaaaat atacttcaat
   123721 gataaaggga aggcaaaggt cgaaatctgt cttgcaaagg gaaagaggct attcgataag
   123781 agagaagata tagcaaagag agatgtcgag agaagaatga ggcagcaggc agatctgtga
   123841 tggatgaacg tctctgtacg tcttttttct tccgaataga gcagtaataa tggctttgaa
   123901 cctcagtagt gttgtggtat aatcaatctg tcttatactg cgaataacgc caatagcttt
   123961 gcaataatta aggggaggtg tatttatgaa gaaagtcgct ttagtagctt tgctcctttt
   124021 tgcggtctca ctgggtttct ctacgcctaa agcgcttata ggtggtgctt ttgggattaa
   124081 tgcaactaat ccagtttatc agcccgagag atggggtgcc ggtgcctttg atctctctct
   124141 tcagcttccg tttactgata agattggggc catgctgagt gctcaggctg caatcaggta
   124201 tccaagcaat aagatagggc ttttcaacgc cgacatttat ttccagatct tcgagacact
   124261 caatctcaag gcgaggttgc ttgtaagtgt gggttccgtc caggattcca acttcggaga
   124321 acagagttgg gagatcggaa ttctcgatct cggagcgccg catcttgcaa tcggtggcgg
   124381 attccaggtt atgacaccga tttcggataa ggtcatccta aacggatttg caaggggcta
   124441 tagagtgaag gattaccaga cccgacagga aggtgatttc ccgggtggag attactggcc
   124501 acttcccgag ttcttctcag taggacttgg agtgctatac gagttctgat caggggctaa
   124561 agatacttat ttgagagagt ttattttggt cgtctttgtt ctactttttt ctatcgtttc
   124621 tttagcggta acgggttacg acaaatttat tcattattct gtaagttatt ccgcctatgg
   124681 cctttcaagt tacttcttgg gtgacatagg cggttttgtt ttctccgcct ctctcggggt
   124741 gggaaaggaa atctgggatt ggttttccgg gaaaggtacg gcggagtacg gagacctgat
   124801 agccgatttc gccggaataa tctccgctta cagtctcacc aagcgtcttc cgttcagacc
   124861 cttgctagta ttcgtattgg tgttctagaa tttaagcagc tctgaaattt cgttctagtc
   124921 cagtaactca acgggaatag atagagagtg ttttttcgca agtgagacaa gtgagtcttt
   124981 tgtcttctgg tctaaatcta tgcccgaagt cacagctctc tctcttgcca tagcctcttt
   125041 ctctccgtgg tagtagattt tgttatgacc gtcggccttc ttgcttgaaa cgaccccatc
   125101 cactatgtgt tcgagatgtt ctataaggga agatcgatca ccgaagagct ccaggctgaa
   125161 gcatccgaag aagtgagaga cacctgcttg agagccggaa taagtttcgg cactccatct
   125221 acccaaagag agaccggcag acaagagatc gacgagaagg gccataccat agcccttgtg
   125281 accaccaaaa tcctcttcgg atcctcccaa aggaagaata cctccggggt ttcgactgtt
   125341 gaagttgttc aaaacccttc tgggagaggt tgtgtttcta cctgtttcat ccactgccca
   125401 gccatttgga atatctcttt ccaccctatc gtaaacctct aattttcccc tggtcacaac
   125461 gctagtagcc atatccagta taagattctt tttcagccca ggaattgcta ctgaaaaggg
   125521 atttgtgcca agaacggcct ccttcccgaa agtcggaacg acgagagggt agctgttggt
   125581 cattgctatt cctatcatct tttctgaaag cgctttttcg gaataatagg ctgagatgcc
   125641 atagtgattc gagtttctaa ctgaaacaag accaatcatc gattctttcg cttttcttat
   125701 tgcaagatcc atcgcgtagt tggaaacaca ttgtcctgga ccgccattac cgtcaactgt
   125761 ggccgaaacc ggagtatcat gtactatcga tggctcggaa tctagcagaa tatttccgct
   125821 atctctttcc ttaatgtatc taccaagtct tgcgacaccg tgtgaaggaa tgccacgcaa
   125881 atcagcttcc aacaaaacgt ccactatttc atgagcggcc tctatgccga aattctcgga
   125941 aagaagaatc tcttcgcaga gcttcctgag gttttcatat gctacgggtt tacccataat
   126001 tatctagtcc tttcaaagta gttccttgct atttttgaat acagcctacg ggccacatga
   126061 acttcttcaa gttccacata ttcgttcgac ctgtgtgcca ttgagggact tccgggacct
   126121 agtactaccg tctcaatccc ttgcgatgca gtaaaggcgc catcagtaaa ataggtcatc
   126181 gtgagctctt ctgcgttcat agccttctct ttaagcactt ctttaacaga ttcggtgaga
   126241 ttgccggaag aggagaaaga ctccctgctg agcaatatga ttttctctga ttgattgtat
   126301 ttgcatagaa cggaatctac cagagcgatg atttctgcag aatctgtgtt ctctgcaaaa
   126361 cgaatgtcga taacacattc ggcctcatcg ggaacggtat tttctttcga tccacctcga
   126421 atgatgttca ggctctcagt aagcccttct attttcccga gaacctgaga aagctcagtg
   126481 taggcactga aaagccttgt aactgcgttg attccgtttt gcggttgcga accgtgcgct
   126541 gatttgcctc tgaatttcag tttaagccat atcgcccctt tttcacctgt cgcaagtctc
   126601 aaggaggtag gctcgccaat gattacgcct gaaacgtcaa aggtttgatg ctcgagaaaa
   126661 tggcgtatcc ctgagcagcc gacttcttca tcacaggtcg caacgagcgc aacattccca
   126721 gaaaaatcgc tgtcttccga aagatctatt agagctgcag ttagcgcgct taacccgccc
   126781 ttcatgtctg ctgaacccct cgcaaacaac ttgccgtcta caagttccgg tttgaaggga
   126841 tccgagtccc agtttgatcc ggccggtacc acgtccatgt gaccggacag gagaaggtaa
   126901 ggtaaggcct ggtctctttg aagtcttgcg acaagatttg agcgattctc tgcgactttc
   126961 tgtatctcaa tatgaacgtt cctgtctaca agcactctct gaagaatctc aacggctcga
   127021 tcttcgtttc caggaggatt tgttgtattt gcgctgcaaa gctctagcag aagcttttcg
   127081 agttccttat cgcccaattt cataatttga ttcccacatt ccagatcgag taatcatcct
   127141 gccccagaac ctctgctttt gtcagttcac cgtttgcaat ccttaataca agatcgaaca
   127201 acttccttcc actctcttcc agagtctctt ccccatagat gatcgtgctg cagtcgaagt
   127261 cgatattgtc actcatcttt tttgcggtct gacggtttcc agtaatcttt atgaccgggg
   127321 ctatcgcgtg gccggtaggg gtcccctgcc ctgttgtgaa gagcacgact gttgctccgc
   127381 ccgccaccat accagtaacg gattcaacgt catatcctgg agtatccata agataaagac
   127441 cgggttcagc atcaactacc tcaccatact cttttacacc aacaatgggg actttacctc
   127501 cctttatcat ggctccgaga gacttttctt cgagagtagt caaacctccc ctgacatttc
   127561 cgggagagat gttgttagga acgttctctt tgtcaactac atcgcgactg ttcttgagac
   127621 tttcgtcaat cattctctgg agcattttgg tgaaccgctg cttcatggac tcatcagaca
   127681 ttctttcgaa gagcaagtgc tccgcgccta ccagttcagt ggtttcagat agtattaccc
   127741 ttcctttctt tttcgccaaa aagtcggcta cctgacctac taccggattg gcggaaagac
   127801 ctgacgagaa gtcagaaccc ccgcattcca ttcccaatac aagcgatgac agtgaaactt
   127861 ctttgcgttc gattttggaa acttccttca ccatatcctt tacaatcgct attcctcttt
   127921 ctattgcttc aacggttcca tagtcctgga tcatgattac ctcggcaggt ttgccatgat
   127981 cttgaattat gtctctaagc tcgtgaggag agactctctc acatcctagc ccgactacga
   128041 gaacggcggc tatgttcggg ttcagagcag tatttatcaa tgtccttctg gtctgtttga
   128101 aatcgtcgcc aagttgtgcg cagccttggt tatggcttgc cacaaccgat ccgggaaccg
   128161 cggacgaaat tcttcttgca acatttgccg agcagagaac acttggcaga actaaaacgt
   128221 ggttcctgac ccctgcactt ccatcacttc ttaggtaccc cttgaaagtc tccatggttt
   128281 cggcttaagc ccgttcacct ccctctttga tctcggttct ttcactagca acattctgga
   128341 tgtgaacatg ctgaccagta gctatatctg cagttgctac acctattttg ttgtcgtatt
   128401 tgattattct ctctttcttt ctgatgtctc taacggctat tttgtgcccg aaaggaattt
   128461 cctcctttgc aactatcgtt cccacttgag aggtgccctt catctcgatc tcttcgcctt
   128521 tagcgacttt ctcgagcacc gtagcgacat tgtcgttaac ggatagcatg atggcctgtt
   128581 tcatcgttaa tcctccaatt caacaaagct tcctctagca gtatattcaa cgttgtttga
   128641 tcctagaata gaacaaatca tacccattga atgtatcaga tgagtttcca gtgcgttcga
   128701 ggccttttcc aggtctccct cgagaattcc ttgagcgatc ttgaagtgtt cctccatttc
   128761 gcagatgatc caatccttct gctgttcaag aagatagtac tttataacgt tacgcattct
   128821 cgtaatgaga atcgagattt tggacatcat ttcaatcagg tattcattgt tgcagttgct
   128881 caaaatctct ctgtgcaacc tataatctat gtcatatggg acgttattct ttgtctcttc
   128941 cggttccgca agatgcttcc gcattctcac tattaggtta gatatcgttt ccctatcgat
   129001 gcgaaagatc gattcactca acgcaagctt ctccagcaca agtcttattg ggaagagctt
   129061 cctcacatcc tcttcaagaa tctccttaac gtagaaactc tttgcaattc caggtgctac
   129121 caaaccgtct tcctttagtt tgtggagagc ccccctgact ggagttgaag aaaccccgta
   129181 gattctcttc agccgatcta tcgtaagcct cgaacctggg ggaatttcca gctctactat
   129241 gctgtttctg atcgatctgt aaagttggta ttggattcca tgaaaatggt ttgtgtctgc
   129301 cgatctattc aaaacgatcc ctccttgaga agagacaact ctctcaaaaa gattctggtg
   129361 tcattcaggg tttcgatctg atcttcatca cagaagacct ctacactgaa gtttcttcta
   129421 tatccggact gtgtcaacaa gttgaggaat ttgtgaaaat ctatcgttcc ttggccaaga
   129481 ggtttgtgat tggaatcggc aaggtggacg tgtttcacta atccaatatt agagctcaca
   129541 aatgaagagt ccacctcttc attaaaatgg tccgaatcga cgagaatacc ataactgcta
   129601 agccctctcg tctcgaagaa atcggcggct tcttgacagt tgttcagaat cgggcagacc
   129661 accttccgga gaggttcaat tagaactgaa agcccttcga gttcattctt aacgaaagta
   129721 tcgatctctg taagagactc ggccagccgg tcgagggact tctgaaggcc atccgaccat
   129781 ataacacctc tcagtctccc gatatttaga tctacgccca gttcataagc ctttctcgcc
   129841 gccgacatga atgaattgac agctcttctt ctctcactga tatcgatatg attcagatag
   129901 agaccggccg ttcctgccat ttccccggtg caaagaaata tacattttgc ggagtgctcc
   129961 ctctccagac gtttgacctt ctctatctct actttgcctg gatcggcaat cataagttca
   130021 aagaatctga acccggcttc gactaacgcc ggaatcgttt cttcaagcct ccccgtcagg
   130081 ccggtaacct ttgcgccggc cggaatatcg tcattggcta ccataaaact cactgccata
   130141 aagctcacct catcccgctg cgctgacaat tgctatagaa agagacgcca ttttcgcaaa
   130201 ttcgtcgtcg cttctcgaag tgagaacgac tggaaatccc gttccaataa gtagaccagc
   130261 agttctcgac ttgcccagat ggacaagtcc ctttataaga tttgccgccg attcgagatt
   130321 tgaacagact agaatatcgg catcccctcc cacttccgac tggatcccct tattcagggc
   130381 cgcctccttt ttcagagccg tatcgagagg cattggtccg tccacaagac attctccaaa
   130441 ctcgcccctc tcggccattt tcgaaagtac ggccgcttca atgagatctc tattagttac
   130501 cgatattctc tcgttgatac caatcactgc aatcttagga catttcaggc ctatcgatct
   130561 cgcaaaacct gccgcgtttc gaagtatctc agctttttct ctcagatcag gagctacgtt
   130621 taccgttcca tccgttatga agagtagttt tgggtgctcc ggaatctcca tacaggatat
   130681 atggcttatc aacctaccgg cagtctttac attttcgctc ctgaataaag ttcttagaaa
   130741 caccgccgta ttgatgtttc ccttcatcaa gacggctggc gtttccccat ttaccgagcc
   130801 tgccgctcgt gatgcgtttt cctcttccgt ctcaccttca actatttgaa cctccttcaa
   130861 gaattctgct ggtgtaatct cgcttatctt ttcgaaacta ccgtacatag tcacttcgat
   130921 cttgctgttc gattcggcga tcgaccttat tgcttcaacg gttgatttat catgtggttg
   130981 aacgcagagt atccttattt ttttgtccag gtcttttact ttatgcgtaa aaacggaaag
   131041 ggaactaatc atctctctca cctcagtaat ccagagcctc ttcagatccg ctaataactc
   131101 tgtgggctcc ttccgcaagg ctttccatct catattcgcc agggaagaga aggactctga
   131161 accccttgag tttggaaaga agcctctccc ttacgaaatc acatttcacc acgccgcctg
   131221 tcaaggcaag agcatctgga aatccatcaa aaactgctag aaggctgtaa gaatacttgg
   131281 cgatattata gatatatgct tcgagagggg cgctgtcgat ttcttctttc attacccttc
   131341 ttgctatact ttcgacgtcg cgcattccat agtgagagaa tagtcctccc cggtgagtta
   131401 ggtagtccag atagtcgtcc cgctctcgag caaactccgc cagttcatgg gagggcaacg
   131461 cgccggctct ttccacagaa aagggtccct ctttatcgct attatagaga tctaccatcc
   131521 tcccattcga atgagcgctc accgaggatc cgcctccaag atgagcaact ataagcttgg
   131581 catcttcata attcttgccc agttttgtcg ctgcttttct cgcgacagcc ttcatgttca
   131641 gcgcgtgcga aagcgaattt cttcttattt cgtttattcc cgtgtatctc gcccactcgg
   131701 ccagttcatc aacacttatc gggtcgacag tgaacgcctg gattccaaac tcctcggcca
   131761 tttctgatgc tatgactact gctgtgtttg ccgggtgcat tccctggagt ccgcgcctgg
   131821 catcatcaac catctcttta ttaaccctat atacgcctga ctcgagaggt ttcagccgac
   131881 cgccccgtgc tgcgacggcg gctaaatcgg aaggtctcac cccgtttaaa tcaagttcct
   131941 ttacaatctg tcgcttcctt attgtatact gatcgggaaa gagaggaaga tcaagcatgt
   132001 cgtcatcaag tggaatagtc tttttgaata cttcttcagt atccctgaag accgcaacct
   132061 ttgttgatgt ggatcccgga tttattacaa gaatcaggct catttcatga agtctcccac
   132121 tatcctcttg aattcatcga tctcaagaac tgtgtttcta atgcggcctt cttttcgaac
   132181 gatttccgtc agctcttcaa tttgatcttc attagcctct attccgagtc tcttcagcca
   132241 gtattttatg cttgcctggc cgctgaaggc accaagtttg acctgtattt ctcccatacc
   132301 gaccagcgaa gggaggtacg gagtcattgc tgcgtttatt cctgctttat tcaacttgtc
   132361 tattgcatcg actacaatgc ctgattcaac ccagaaaagg cgcttgccta cgacgggttt
   132421 gttgtttgcg acctgaattt tcgagatgtc ctcaaccagt cttgaagtgt agccgatttt
   132481 cgatagatca ataccggtat ctatgcccat tagcagctta agaccggcag ccacttcctc
   132541 ggtggccacg tttccggttc tttcgccaag gccgtttatt gagctatgaa tgcccgatac
   132601 gcctgccctc actgcggcga aaacagatcc cattgcaagg ccaaattcgt tgtgtacgtg
   132661 gaattcaaag ggaatgtcgg gaatagcctt tctaagttca gagaagatga attcgattgc
   132721 aaatggcgag gcgacaccaa aactgtctac gaagaccata ctttccggct tcgcctgctt
   132781 agccacttct gtgaatatct taagaacgta gtcgaatgaa gatcttgtcg catcccatcc
   132841 catgaaagta ggcttcatgc cctgttcctt tgcgtaaagt attgccgaaa ctactctgtc
   132901 cacgagcttt tcgggggaga ctccatacac gagttcattg tcataaggat taactgcgtg
   132961 ctcaataacc acccgggtag caccacaatc aagagactcg tctatgtctt ttttcaaaga
   133021 ccttgcaaag ggaacgatct ctgagtcgag cttcatatcg actagccttc ttattgctct
   133081 cttgttctca tccgatacaa tcggcattcc cacctctatg gatttgacac ctagttcgtt
   133141 aagtgcaacc cctattctta ccctctgatc ctcgttccat gtaaggccac aggtctgttc
   133201 accgtctctg agagtgacat cgtgtatgta gatactgtct tttgcgtctt ttccccttat
   133261 tttgttttcg tagtttagag ggcagaccca acgggaatcg tcgaagtatg gcgttccgtt
   133321 caatttcatg tactcaaggt cttttggttt catgctatac ctcctcagta gttatctctg
   133381 gcaacacgag tgagttctga aggacagaaa cactcttgcc aggacaaatc aatcggtcaa
   133441 ctacagattg aatatctgga ttgacacgta atcctagctc atcaaatttc tcagcaaact
   133501 ttgtgtcgct gacaatgtga accttcttct tctgcagtac ttttcttacg gcgtaagccg
   133561 ctacataagc gaggggatct tcggtggtta tcatatcgag tttctctaga atttcatttg
   133621 cctcaagttt taagagagaa gcgaaaagtg gatggtctcc aaaaccctcg tcaagtgaag
   133681 aaaccataac gatatcccca ccatctttgg taatcaagtc cgacgtgtac agagccttgc
   133741 cgcattgcca gaaatcagtt acagaaggaa aggaagaaat gaccgtaaca tctgtccttt
   133801 cgcgatactg ccgaagaagc tgctttctcg cgagctctac cccttttctg agggctagca
   133861 cgtggttccc cgcaacggtc ccggcagttg atccgttaat atcgtagtag aaatttataa
   133921 tgaaatcaag ccctactcga cttccggttt cgtctatcag ctctctgaat tcattttcaa
   133981 atactcccac gtctgccttg cttttcaata ttgccattct gtgagatttg aagacacttt
   134041 catatgaggc gactcccgga aggactatct ttgcccctcc cgaccaaccg gagaatctgt
   134101 gaggaacaat ggaacctatt ccaactgtga ggtcggcctc tatagcttca tttacttgaa
   134161 tcggtacctc aagaaactcc ccgaaatctt tcatcttggc agtctcttcg gctttatgct
   134221 gaacgattct tgtatctgaa gagaatactc cgaacttttc ttgaagctct tcgggtttca
   134281 tctgcctgtg ggttccggta gcgataagga gagaaacttt gtcccacgaa attccgcgac
   134341 tttcgaagag ttttcttaga aggtccagag tttccagtac gggtgaatgc ctcgtgtggt
   134401 cttccacaat aaacacgata ctttttgctg actccgcgag actagtaagt ggtgcagtgc
   134461 cgatcggcac tgaaatcgat tcggttaagg agcttccggc ttcagccggc tcagcggaaa
   134521 tcgcgggaag tattacttga cccgcgggca cttccaactt gactcttctc ccttctctgt
   134581 aatctaagga aaactccatc ttacttgcca ccaaagtaca tttcgcggat tcgctcgtcg
   134641 cttttcactt cgtcaaccga gccttcaagc tttatttgac cttgagaaac tatgtagact
   134701 ctgtcgctca ttggaagtga aagctttgcg ttctgttcga ctatcagcat cgtaaggcca
   134761 gtatccttga caaatttctt cagggtctcg aatattcttt tgacgattac cggcgcgaga
   134821 cccatcgatg gttcgtcgat catcataatt accggatcgg acacgatcgc tcttcctata
   134881 gcaagcattt gctgctctcc tccggaaaga gtacctgcca tctggttctt tctagattta
   134941 agctcggaaa agagttcgta aactctctca atgtttgggc gagccttaga aagcccgact
   135001 ctggtggcgg cgacttctag gttctcttgc acggagagct ccgcgaaaac ctgtcttcct
   135061 tccggaacgt gaataactcc aagtctagta atcttgtggg gagcaagatg ctggatctcc
   135121 tcgccgttca gtagaatttt gctgccgggt tcagaaccga ccagtccaga gatgctcttc
   135181 aagagagttg tctttcccgc tccgtttggg cctagcacgg aaacaacttc tcctttcatc
   135241 accttaatag atactcttct gagagcagga acttcattgt aggagacaga taggtctctc
   135301 gtttcaagca aagtcataat caatcctccc ccagataggc tttgagaaca agcggatctt
   135361 tttctattac atcaaaggta cctttggcga ttatctggcc aaagttcaga acatataccc
   135421 agtcggccac agccttgatg aagtccatag tatgttccac gagcagagtc gttatacctt
   135481 tctctttgag aatcatcatc aggttcatta tcactttgaa ctcatctcta gtcattcctg
   135541 cggcaggctc gtcgaggaaa atcaactgag gttccagagc taaagctctt gcaatctcta
   135601 gacaacgccg taatccatat gccatgccct tcggggaatc ataagcatta tcctcaagac
   135661 ccacgagctc caaaagttcc atggcctttt ctctgttctc tttttcttcc tttctgaaac
   135721 tcggagttcc tagaactacg ctcaaagggc cggatttata gaaagacttt ctggcgacaa
   135781 gtacgttctc aatcactgag agattctcga agagtcgaat cttctggaag gttcttgcaa
   135841 tacccaacct gctaatcctg tttatttcca tccccgttat gtcctgatcc ttgaaatata
   135901 cctttccact tgtgataggg tgtactcccg ttacaacatt aatgaacgtt gttttgcccg
   135961 agccgttggg accgattaag gctgtgatct cgccttcggg aatccctatt gacagatcat
   136021 tgttagccac aactcctccg aatctcttgg tcatattttc agttctgaga atgtccattt
   136081 tcaccccacc ttacaacgtc tttgtgttgt ttctctttac cctgaaaacc tttctgaata
   136141 agctttctag gatagggcca agtcccatag gcataaatat tacgatgaca atcactatta
   136201 gagaagtgac cattagatag taattttccc agcctccaaa tctcagaaac tcgggaagaa
   136261 gcgtaagacc tattccgcca acaactccac cactcatgtg cctaagaccg cccacgacgg
   136321 ccatggcgac gaaatttgag gattcaaaaa aggtgaatgg ttcgggtgag agaaaaccgg
   136381 ctagatgagc atacagtgct cccgccagtc ccgctaatcc tgcagaaaag ccaaaagctg
   136441 cgatcttgag ctttttcgta gatattccac aagtctcggt agcaacgtca tcatcattca
   136501 tagccagcat agctctacca aagtgcgagt gttttatgcg gaacataatg aaaactgaaa
   136561 ggagcaataa ccctccaaat agccaaaaga cctgcccaaa ggatagcatc tcgcccccaa
   136621 acgaatagaa tggaattcca aagaatccgt aaggaccacc tgtgattggc gttaagttta
   136681 ttaccagcat ccagactatc tggccgaatg caatggttac aagagataag aaaaaggcct
   136741 tgagtttgaa tgatggaata cttaacagga atccagcaat aacgctaatc actataccgg
   136801 caattacgcc aacaacgatc ggcatcccaa gcttgaccgt gaaataagcg gaggagtagg
   136861 caccaatggc atagaaaccg gcctgcccta gtgagatttg accagcgaac cctgtgagaa
   136921 aaactaatcc agtggcaaga atcgcatgaa ttaacgctct gttcataagg taaagatggt
   136981 acgagttctt cacaaaaagc gggaacagta ttattagtag aattgctcct attacgaaaa
   137041 tcgtcttctt catcttgtgc cccctatatc ttctttctct tccagttgaa tatcccggaa
   137101 ggtttcagta tcagaacggc tacaagaacc gagaaggaaa ttacgtctct atatgttgaa
   137161 gagatataga gggtcgagaa gttttcgagt aaaccaagag atattcctcc gagtatcgtt
   137221 ccataagggt ttgtcaatcc gcccagtact gccgacgcaa aacctttgac ccctatagaa
   137281 cctcctccaa gttcaagaga gacaaagtaa ataggcgcaa ggagtattcc ggcaattccc
   137341 acgacgactg tcgccagaat gaaggataga gaggtcgtta ccttcacgtc gattcccatg
   137401 agagctgccg cttctctatt ttgagcagct gcacggaatg attttccaat aagtgttttg
   137461 ttaagtataa aatagatgaa aaccatcaga attgcaatta caagatagat gttcaagtag
   137521 agagagggaa ttcttatgcc accgagtgaa atgaagtcgt tgcttcctcc gaataccggc
   137581 gggaagggtt ttggtagagc cccataagtt acaattataa agttgagcca aaaagtgcac
   137641 agcataagtg tggcaacgac gaattcgaga cgcccaccgc ccctctttct gattggctca
   137701 tagatgaata ttctgagccc caatccatat agccctatga tggctacaga caagacaaat
   137761 gatatgaaga accccaaatt tagttgagta tagaaactgt aaaataaata agccgccaac
   137821 attgccgacg atatgttagc aaagtttatt cttgagatcg tcaaccagag gaaaactacg
   137881 ccgagtccta cgagtccata tgtggcaccg atcgaagccc ccgaaactat gttttggaga
   137941 atgtcttgaa gcattgttca cactccttct agggaagtaa acgggaggta ggcaggacgc
   138001 cccctcccgc aaagaggctt agtacaactt agtgattatc tgccagtctt ttccgtcgaa
   138061 ctgggagatt agacagacgt ttgtgaagtt gtggtgttcg tcgcacttga gttcgccctg
   138121 aacaccgacg atgttcgctt cattcgcaag gtattcagca agcttagtac cgtcagttcc
   138181 aacattcttg agtgcatctg ccagaaggta cattccgtca tagtaagcct gtccagggtc
   138241 gcttggttcc ttgccatccc atctggcctt ataggcttca acaaatgcct gaactgcagg
   138301 atcgggatct attggattga atccctgaac ggagtatacg ccaatcgccg cttccggcgc
   138361 tagatctgta aacgcaggaa cgccccatgc agagaaccca attatgggga catcgatctg
   138421 caactgtctg agttgattta ctgttacagc agcctcagat tcgtgtgtaa gtcctatcaa
   138481 tccatcgaac ccggcgttct ttagtttcag aagctgacca gttgcatcct tgtcgccttc
   138541 cttgatctgc tccattgcga ctatttcgat tccgtacttc gggaactcct ctttagcaac
   138601 atcgagagct gcaagacagt aatcggtcga gccatagata actccgggct tctcaatgcc
   138661 caactcctct actgcatatt tcacgagctg aatgactttg actttgtcat ccgccctgag
   138721 tctgaagagg aagtcgttgg gaatcaccga atatctgaat gaaggggaag tcgctccgac
   138781 aagcattgga accttcgcct gctgtgctag ctgatgaacg ccaatgactg aactgctcat
   138841 gttcggtcca aagacggcca ctaccttatc ctggaaaagt gctctcctga taacatttgc
   138901 tgccgtgctt ggatttgccg catcgtcata cacaaccatt tcgagcatgt cgccattgat
   138961 tcctccagcg gcattgatct cttctagtgc caactgcgca cccattttga tgtagtcgcc
   139021 aagaatcgaa cccagtcctg tgaatggcgc cacaagagca accttaatcg ttgaacccag
   139081 cgccgcaacc ccgattagca agacaatcga gactacgagt aacctgttta gcctcatcca
   139141 aaacacctcc atgtgcggga cagtattaat tccgccccgt tctcttagaa gaaagattaa
   139201 taaacgatta acatgatgcg ttatacatta tgcacagtat agcattcctt gagtcgatat
   139261 cttaactcga tcatagccct taaggatcaa atgaattgaa ttattctaga ttatctttct
   139321 ctactttgac ttctcgcaag aataagacat tcttcaggac aagtgtggaa cgttatttgc
   139381 acagactgtt ttgtttacgg gtcatgtgtg aacccatgtg ttcgatgacg attatccttg
   139441 acgttattgt cattggagag accaacttct ttcttcgcct ttgcatccat gggttataat
   139501 tgttttcgaa attgggcttt gcattgaagg tttctggggg tgttgtttgt gagagcagtt
   139561 caaatggttt gtcttttcct ttttgtcatc acattttctc tattggttgc cgtggaaatt
   139621 gatctgggcg tgagtgctga tataacccag tcagatgaga cgagcctaag ttgtgcgcga
   139681 ctttctcttc tagccagtgt tggcccacta tctatcgatc tgccgattgc gcagatttgg
   139741 ccttcagaaa gaagacttga gcttgccgat cccatgttaa gttatgcagg cttgaaccta
   139801 aaacttccga taaccgttct ttacataaag gccggtttgg gagttcagtt gaaaggtttg
   139861 atagatctag tcggaggaag tatcgaagga tgggatgtcc cggcgagagt tagagctggt
   139921 ttaggcctat cggattctgg cctctttctt gaaggcggag taaatctcga tttcacacct
   139981 tctctcgaat cattgagttt ctgtccttat attgcggccg gatttgtttt ctagctagat
   140041 caggcgaagg tgtttactga attcttcttc aacctcttga aatgtatatt tctcaagcca
   140101 ggattgccag ccgaatttca agaagatatt ctttacctga aaggagtagt tcagaggatc
   140161 cagatggact tcttcgactc tttttgataa ctcaagtgca actcttgtcg cgtccatagg
   140221 aagaacaggt cctacaaagg ctcccgtctg aattccgtta tctcttaaga aatcaatcgt
   140281 ctcaagtctc tcttcaaagg aagaagaacc cgattcaaga accttcctca catcttctct
   140341 atccgttgtt atcgttacta agactttcgc ttcgccttcc gcagcctgga gcaagtccag
   140401 gtctctcctt accaaagagg atttagtcat caagtagacg ggaatcctgt gagagacaag
   140461 aattctcagt atcgctcttg tgatttcgta tctcttttcg agttgctgat aaggatcaca
   140521 ggttgttctg agaaaaactg gccacttggg atgtctgata atttctcttt tcagaagaga
   140581 tgcgaggttt tttctaactc tcacatccct tccccataca cctccgtagt tcttgaagga
   140641 gccaatgaat ctagcaaaac agtacttgca tccgatgctg catccaacgt aaggattgat
   140701 agtgaaatct gttaatggaa tattagactt tgttaatgcc ctttttgcaa gtatttcttc
   140761 caccatatac taatagattc tacatgtttt tcttctcgat aaacgatttc cgttaagtgc
   140821 atatcgcgcg acttcatcca ttctcaagtg atataattaa cggaaatggg aggtgttact
   140881 gtgttgaggg ttatcaatac tgacaaggcg ccggcagcag ttggtcctta ttctcaggca
   140941 gttgcggcgg gaggttttct ctttgtttct ggacagattc ccctcaaccc gttgactggc
   141001 gaaatggtga cggattttga agaagcttgt aaacagtcac tagacaactt attctcggta
   141061 gtcgccgccg gcgggtcgag cccagataat atcgtaaagg tcaacgttta cgtaagggat
   141121 atgggtaagt tttcgattct aaatgagatc tatgctactt actttaaggg tcacaaacct
   141181 gccagagccg tagtagaggt ctccaacctg ccaaaaaacg cgccggtaga aatcgaggct
   141241 gtggcgatta taccgtaatc aggaggatag aatggcaatt tcgataaaac tgcccgatgg
   141301 ttcgataaag gaatacgaat caggtataac accggcggag attgcaaagg acatttccga
   141361 gggacttgca agacgtgctt ttggagcgat tgtcgacgaa gaactttggg atcttgaacg
   141421 gcccatcgaa aaagacgctt cggtcaagct tgtcctggac aaagatcccg agtcatctcg
   141481 tttcttcaga cacactatgt cccatatact agcccaggcg ataatgagat tgtatggcaa
   141541 ggagaaagtc aagctagcaa tcgggccaac catagaaaat gggttttatt acgacataga
   141601 tcttggcgag acgagaatca cggaagagga tttaccgaag attgaatcgg agatgaacaa
   141661 gataattaag gaagatcttc cggttgagag attcgaactg cctgtatcag aagccattga
   141721 acttatgaag agagaagatc agccttataa agtccagctg atagaggatc tggttaagga
   141781 cacgggtgct gaaaaggtta ctttctacaa gcagggcgaa tttgttgacc tctgcagggg
   141841 tcctcacgtt tcttcgactg gtaaggtaaa gtatttcaag cttacatccg tttccggtgc
   141901 gtactggagg ggcgacgaga acaacaagat gcttcagagg gtttacggca ctgccttcgc
   141961 aaaaaagagt gatctcgaag actatctgag gatgatagaa gaggcaaaga agagagatca
   142021 caggaaactt ggcccggcgc ttggtctctt cacaatcaat tacgaatatg ctccgggtat
   142081 gcctgttttt ttgcctaagg ggacggagat gcttaatcaa ctgcttcagt tttcgagaga
   142141 gaagcatgtt gaagatggtt acagggaagt tagcacccct cagattatgt cagatgcgct
   142201 ttggagaaca tctggtcact gggatcatta cagggacaac atgtatttca cggagaaaga
   142261 agatcagcag ttcgctgtca aaccaatgaa ttgccccggc cacataatta tatacaagtc
   142321 atcatcggta agctatcgtg atcttccgat gaaactgttt gaatttggaa aggttcatag
   142381 atatgagaga tctggtgtct tgcacgggct gttcagagtc agaggatttg ttcaggatga
   142441 tgcacacata ttctgtactc gtgagcagat tcaacaggag ataatgggag taatcgattt
   142501 cgttcagaaa atatactccc cattcaactt cgagtataga gcagagcttt cgacgagacc
   142561 cgaagattac atgggcgaag ttgagctgtg ggatatcgcc acaaaggcat tagaagattc
   142621 acttgtggca aagaaaatgg agtttaaggt aaatgaaggt gatggagctt tctatggtcc
   142681 taagatcgat tatcatataa aggattcgct tggtagggaa tggcagtgtg ctacaattca
   142741 gcttgatttc atgatgcccg agcgttttgg gatcaagtat gttggacccg ataacgaaga
   142801 gtatcagccc gtcatgattc acagagcgat attcggctcc gttgagaggt tcatgggtat
   142861 tttgattgag catttcgccg gagctttccc cgcttggctt gctcctaccc aggtttcagt
   142921 gatcccgata gccgacaggc atgcagaata tgcggaatcg gttgcggccg aattaaagtc
   142981 cagaggtttc agggttgaag tcgatgatcg ccagaagtca actaactaca agatccgtga
   143041 ggctcagctg atgaaagtac cttatatgat catcgttgga gacagagaga aagaagacgg
   143101 gaccatatcg gtaaggctga gaagtgagaa ggatctgggt tcaattgctc tgcaggaatt
   143161 catggataag ctttcgcaag aagtcaacat gagaagcgca acaagcattt tcgaatgatt
   143221 acaagacccc tgcacctcgg gggtcttgct ttcgtagctt acaagtcctt gaaggggggc
   143281 gattaaaacg aatatcatcg acataatacc ggtcgcacgt ggcgacagac cagccgatgt
   143341 tcttcttaag aactgcaaga tcgtgaacgt tttcagcggg gagatagaag aggccaacct
   143401 ggctcttttc aagaaacgca ttgccggaat cggcgactat gaagatgggc tagaggttat
   143461 agatcttgaa ggttcttatg tggtgcctgg tcttatcgat gctcaccttc atatcgaatc
   143521 atccatggtt tcgccggtcg agttttcgaa gacaatccta gcaaaaggca caaccacagc
   143581 gatagccgat ccccatgaaa ttgccaatgt aatgggcctt tccgggatag aatacatgat
   143641 caagtccaca gagggagttc ctgtgaatct ttacatcatg atcccttccg ccgttcccgc
   143701 atctgccttg gaaacgtcgg gggcaaggat cagcgtaatg gatatgatag gttttgtgga
   143761 gaaattccag aacagagttc tcggattggg cgaagtgatg aacttcccgg atatcataaa
   143821 cggtgatcgg gattcaatag cgaagattga gttgattcga cacaagtaca agaagataga
   143881 cggtcacatc cctggagttt tgggtaagcc tctaaatgcc tacatatgcg cttttgtaag
   143941 gtcggaacat gagtgcactt acgtcgagga agcgagagaa aaactttcca gaggtatgca
   144001 gatacttatc agagagggaa gtgtggagcg aaatctagtt gctcttctcc ctctaataaa
   144061 cgagaagact tatccctttg tatcattttg taccgacgac aagcatcccg acgacattat
   144121 cagagagggc catatagact acaatattcg gaaggccata gagcacggta tagatccaat
   144181 aatcgctata agagcagcga cgataaacac tgccagacat tacaatttgc gttcgatggg
   144241 cgctattgct cccgggtaca aggccgacct cgtagtggta gacaatctag agtcgttcaa
   144301 tccgagaatc gttttcaaag attctagaat cgtagctaga gatggaagac tggttattga
   144361 tgtcatctca aagagttttc cacaggaaag gacaaataca ttcaagtgcc agaagatttg
   144421 tgaggatgac ttaagagtta aggctgaggg aagaaaaatc agagtaatag agcttcttgg
   144481 aagcgaggtg cttaccggag gcctaataac tgacgcaaag attgagaacg gctgtgttgt
   144541 cagcgatgtc gatagtgacg tcttgaaact ggcctccgtc tgtcgctatt gcgaaggtaa
   144601 gtctatgtct gtcgcgtttg caaagggctc cgggctaaag aagggggcag tggcaacttc
   144661 tgttggacat gattcgcaca atcttggtgt attgggagtg aatgattccg atatggtctg
   144721 tgcggcaaac agagtgatag aaatgggtgg agggttggtt tccgtcgttg acggtaagat
   144781 aacatccgag cttcctttga agatcgctgg cctgatgtcg gatcttacca gtagggaagt
   144841 cgcagaaaga ttaacagaac tgaaagaagc tacaaagatt atgggtagca ctttacccga
   144901 tctcttcatg acgctttctt ttgtgcagct ttcagttatc cccaagctga agctgacgaa
   144961 tctcggcctt gtcgacgttg aaaaaaatga ttttgtcgcg ctgttcgtta aggagggtga
   145021 agatgcctga aagacacgag aaggtggtcg gcaaaaaaat caacagagtc gacgcccggg
   145081 agaaggtgag cggcagagca aagttcgcag cagatgtgtc ttttgataga caggtgaact
   145141 gcgctcttgt tattgcaaag aaagcacatg gagttctgaa gagtattgat gcttcttccg
   145201 caaaaaggat tcccggtgtc gagggtgttt tcacctggag agacgttccc ggggaaaacc
   145261 agcttggcga tgtgataaag gatatgcctt gtctagtcaa ggagggcgaa agagtaagat
   145321 tttacggtga tgtagtagct gttgttgctg caagcagcaa gaagatagcc gaggaagccg
   145381 ccgagctggt aaatgttgag atcgaagagc tcaaaccggt tctaacaata gaagaggcaa
   145441 ttaaggatga aatcgttatt catggtaagt ccaatatagg cgtttcgaga agaattcgta
   145501 aaggaaatgc ctcgaagttt tttgagaatt cagagcacat tttcgaaggc gagttctacg
   145561 cccactatca ggagcaggct tatatggaga cccagggagt aatagtggtg cccgacattg
   145621 actacgggtt cactgtttac ggaacaatgc agtgtcctta ctatgtgcaa ggggccgtgt
   145681 cgaatgtctt gggcattcct atgagccgag taagagttat tcaaaccgaa accggcggtg
   145741 catttggagg aaaagaggac attccttctt atgtcgcttc atatgcagcc atagtggcct
   145801 ataaccttcg aaggccggcg aaactaattt attcaagaga aatagacatc cagacaactt
   145861 cgaagcgtca tcccataaag tctcactata aagttgccct tgaaggaaag aaaatcaaag
   145921 cgatgacgat aaaggcatac atggatatgg gagcgtatgc aacccttact ccgatagtta
   145981 tctttagaac gcttgttcat gcggccggag cttacgaaat agaaaatgtt gacgtcgatg
   146041 tttatgcagt ttatactaac aaagttcctc cgggagcctt cagaggtttc ggttctcctc
   146101 aagttctctt tgcggttgag agcatgctgg atgatattgc tgtccagatg gggttcgacc
   146161 caatggagtt cagagaaatt aacactctta gaccgggttc caggacctct accgatcatc
   146221 tgttagttaa cagcgttggt gctccagaga ctctttcaag agttaagaag gcttcgaaat
   146281 ggaaggaact tgtgaaagag acggaggaat tcaataagtc tagtgaagaa gtaaaaagag
   146341 gggttggtct ctctcacatc ttctatgggg tcagcctcgg tgcaggaggt caggctctcg
   146401 acaagtccgg cgcacacgtg ctgatagaca gggatggttc tgtcgaagtg aggattggcg
   146461 gaacggaaat gggtcagggt gctaagacgg tgatctcaat tatcgcggcg gaagaattgg
   146521 ggcagagcat agacaagatt gttgttcatc aaccggatac cgccttcgtt ccagacagcg
   146581 ggcccacagt cgcatcgaga accaccattt actccgggaa cgcggtgaaa aacgcctgcg
   146641 cttctttgcg ggagaaattg gcccctgtgt tctgtgaact caccggtagc ccgctctcat
   146701 ctgtgagatt tgaagaaggg tttgtttatg gcgaaagtgg gaaaaagttg tcattcgatg
   146761 aagtagtcga acatgcctac aagagaaatg tgaaactttt cgaaacaggc tggtatgaga
   146821 cgcctaaact gaaattcgat atggacaacg gaatcgggga ggcatacgtc acctactcat
   146881 atgcaagcca ggtcgttcag gtcgaggtcg atctggcgac aggctacagc aaagttatca
   146941 aagctttcac agcccatgac gtcggtagat cggtcaatcc tgaaggagtg attggccaaa
   147001 ttcaaggcgg ttttgcgcag gggatgggat acgcaatatt cgaagacatg aagcaaaagg
   147061 acggaaagat catttccgac aatttcaaca cttacatcat tccgactgtc cacgaacttc
   147121 ccgaagaact ggtcattgat gttgtggagg atccctttcc agagggacct tacggagcga
   147181 aggggatagg ggagccttca ctgatgccgg tacctgccgc agttgcaaat gcaatttcta
   147241 tggcaatagg gaggcgtgtt agaaggattc cggttacccc agaatatgtg cttcagctct
   147301 tagaaaatgg gtcgtcttta caagaatagt tgttcgtaaa caaggaggat tagcatgggt
   147361 aagttcgtac tcggtccaac attcgaggag atgttgcatc cagagaagat cgatagaaaa
   147421 actagagaga ttgcatggaa gatgagaaag gaagatcccc tggatccaat aaacctttac
   147481 aacatttcct ggagagatat cgataacagg atctactatt ttgttcttcc aaaggagttc
   147541 actggagtag atgcgaatat cgttgtactg tatggtaaag acttcccaac tggaagccac
   147601 aaggtaggag caacgtattc gattttgatg gaaaaagtgt tgaggggaga agtggatcct
   147661 acttaccata ctctggtttg gccatctact ggcaactacg gaatcggtgg tgcatgggtc
   147721 ggctgtagaa tgaagtttga ctccgtggtt attcttcccg aagaaatgtc ggcggagaga
   147781 ttcaacatca ttagagagta tggagctaga gtattcgcaa ctccagggtg cgagtcaaac
   147841 gtgaaagaaa tctacgataa ggcgaaggaa cttaaagcgg ccaatccgga aaaagtacgg
   147901 gtgctcaatc agttcgatga gctcggtaat tatagatttc attactacgt aactggaaat
   147961 acgattctgg aaatgatgga cgaactcagg cttggcaacg gaagagtttc ggcagtagtg
   148021 gcaacggtgg gctcggcagg aactattgca tgtggggaca gaataaagga agtgtatccc
   148081 gaagttaaaa tcgttgctgg agaacctgtg caatgtccga ctctatcact taacggttat
   148141 ggaggtcacg acattcaggg gatcggggat aaacacgtca catggattca caatgtaatg
   148201 aacaacgatg cagttgttgc gcttgacgat atcgagtgta agaaacttct tcaggtaatg
   148261 accgacgaag aaggtaagaa gttcttgaaa gagtttctta aagcagacga tgtcgataca
   148321 atggccgaca tttttggcat ttcaggagta gcgaacattg tcggtgcgat aaagatggcg
   148381 aagtactaca gaatgagcga caaagacaac atcgtgacta ttgcgactga caacatcgac
   148441 cgctaccact ctgtgatgga aaacatgtcc aaaacttatg gacaactcac cagggatgaa
   148501 gccagacaca gatttgaatc atatgttctt ggtgcaaaga cagactggat attcgagggg
   148561 actatggata atagattgag atggcacaat ctaaagtact atacttgggt tgagcaacag
   148621 ggaaggtctg ttgaagagct gaacaagaca atgtcacagg acttctggaa ggcagaaagc
   148681 ggaaagattg aagagattga caagctcctt ctcgattacc gcgtaaggaa caaagaataa
   148741 ttagtatgac tatttgtttt gttttttgtg agctttattg tacaatgaaa acacggtggt
   148801 actgacgaag agaaaactgc ctaccttttt cgggggcggt tttttctttg tgtatctgag
   148861 aagttgcttc agctctagat tctatcccaa ttatggaggt gattcttttg aaaaagtttc
   148921 ttcttgtttc ggtcgttttg atgttctcac ttattctctc agcaactact atccagttct
   148981 ggcatgcaat gggtgggtgg agaatcgatt tcctgcagtc gcaggcagat gcgttcatgg
   149041 agttgcaccc cgacattgaa gtcgaagttc agtacaccgg aagctacaga gatactctga
   149101 acaaaatgat cgcagctgtg caggcaggaa cagctcccca cgtggttcag gtattcgaaa
   149161 tcgggactca gatgatgatc gacggcggag ttgcaatccc cattgaagag atgatggagg
   149221 ccgacccaac tttcgatatc ggaaagttca tgccacaggt tctcaactat taccgggtaa
   149281 acggcaaact ctattcgatg ccgttcaatt catcaaacgc aatcgttttc tacaacaagt
   149341 ctctctttga aaaggccggt ctagatccgc ataatcctcc gactactttt gaagatttca
   149401 tcgaagtggc caggaagctt accataaagg actcggctgg aaacgtggag caggcaggat
   149461 ttaccgtatc tcttaattca tggttctttg agcaactact ggcggcacag gacgccccgc
   149521 taattgatca gaacaacggc agaaccggaa gaccaacgaa ggcagtcttc aatactcctg
   149581 aaggtctggc gatcttcgag tggtggaata ccatgtcgca ggaaggtaca atgataaaca
   149641 cgaagtttga agactggacc gctgcaagaa acctgtttat ttcgcaaaaa gcggcaatgt
   149701 tgatgacttc cacttcggat gtcgccctga tgatggaatc cgccaagacg aacggttttg
   149761 agcttggtag tgtctttttg ccaagaccct ctgattcgaa agctggagga gcgatcatag
   149821 gcggtggaag tctctggctt atcgatggcc atccagacga agaaaccaag gctgcctggg
   149881 agtttatcaa gttcatggct agcaccgagc agcagattga atggcacaaa gccactggat
   149941 acttcccggt tagaaaggat tccgttgaga aactcgttgc cagcgggtat tacggtgatt
   150001 acccggatca cctgacagcg ctcttacaac ttctgctttc agtccagagt tacaatacta
   150061 acggagcaat aactggagca ttccctgaga taagaaccgt ggtaacaacc gcgattgaaa
   150121 agatgatcgc caaagaaatg actccttccg aggcgctgaa gtacgccgaa gatggagcaa
   150181 ctcaagcaat tcgcgactat aacgaagcca attactaatg ctcttttatt ggccggagcg
   150241 ggcgatcagc ccgctccgct tgatgctgga ggtgtagaat gcgaaagtgg ttaccatacc
   150301 ttctgctggt acctaccttt ctgattatcg ttttattcat ttattatcct gcagtagaag
   150361 catttagaat gagtctctac agagtgtcta cctttggtaa caaaacgaca tttgtgggaa
   150421 tccagaatta cataaacctc ttcaacaatg agcagtatct ttcctcagtg aaactaactg
   150481 tcatctatgt cgggctttct gttacattat ctattttcct tggtttcata atcgctatgc
   150541 tcctcacaca gaacgttcca ggaacgaaac tttacaggac ctttctcttc gcgccttatg
   150601 ctatttcccc ggcgatagcc ggcacccttt ggaccttcct tttgaacccg gttgttggcc
   150661 acgtaaacta tgtattcatg aaactatttg gacttcaggt tgaatggtta acttcaaagc
   150721 cttacgcgtt ctacgcgctg atctttgcaa cagtgtggaa gatcttgcct ttcaacatga
   150781 tcttctatat tgcaagtatt cagaacgtct cgggagacct tcttgaagca gcaactcttg
   150841 atggagcagg ggtcatgaaa aagacctgga gaatcatatt tcctttagtt tcaccaataa
   150901 cattctattt ggtcatcatg aacattgtca gcacgatgtt cacgtcgttt gcaatagtcg
   150961 atgtaatgac gaagggcggt cccggaggtt acacgacaaa tatgatatat aggctttatc
   151021 ttgacgcatt cacttatcag aaaagaggac ccgcctcagc gcaaagcgta ataatgttca
   151081 tgataatggt cgtagtcaca atagtctact tcaccgttgc agaaagacga gttcactatc
   151141 aatgaggtgt agtcatgaag aagtccacta ggaagttagt taatacaata atcatagaga
   151201 taatattgat cacagtatct ttcatattca ttatgcctgt tattttggcc ataactatga
   151261 gccttcagcc gcctcaaagc gtcttttcat ttccaccgaa gtttttccct acggcctttc
   151321 acatccagaa ttactttgat gctttcaaga cagttccttt tgcgcgttta cttctgaaca
   151381 gcgcaattgt atcgcttgcg attactcttg ggaaactcgt tactggaaca cttgcgggat
   151441 atgccttttc gaacttcaga tttgcaggaa gagggctcgc cttcgggttt ctttttgcaa
   151501 ctctattctt gcctgcggag acgataatga tcgttccctt gttctcctta atgaataaat
   151561 ttggatgggt gaatacatac tgggccctca ccataccttt catggcgagt gcaacaaaca
   151621 ctttcttaat gagacagcaa ttcatgacta tccccctttc tcttgaggat gcggcaagaa
   151681 ttgatggagc cggaccaatt aaatactttc taaaaattct cttgcctctt tcaaaatcga
   151741 tcattgcagg ctccgcgatc ataaactttg tttattcatg gaacatttat ctctggccgc
   151801 tgatagttac gatggaagac aagatgaaaa cagtgcagat cggagtcaag atgcttatcg
   151861 atgctgagtc ggcgaacaac tggggaatca ttatggccgg tacgatggta gcggtgattc
   151921 cgacccttgt agtcttcttt cttttgcaga atctctttgt gaaaagcctt gtaggaactg
   151981 gtctcaagga atagtcagac tcgaaaggaa agccaggata gaatgaccag agaaaacctg
   152041 cacggagcat attgaattct gatgagaatt gttttgaagc tgacctagtg ctggaatatc
   152101 tggctacata acctgaatgt aaagattact tgggtttaac ctttcagaat cgattatctc
   152161 tttcgttgct aacacccatt agatttgaaa tagagtcatt tagtatcaaa tatttcgaag
   152221 cctgtatctg ctgtatagaa tctttgtaac cgtccaggca gctgtacggg gtgatcattt
   152281 tacccgttct tctctcaact gcttcactgt tatcgagaat tccgtcgctg gacattatga
   152341 aaatcctgtt attgtcgatg aatgagcctt cgggaacata atgagtgaat gcggcaaatc
   152401 ccgacgacga attaagaaga tctcgaccga acagtattcc gctcttttct acgatcccga
   152461 tgagattaag cagtgtggga aagaaatcaa tctgaccgcc tggtttggag ttcaggacat
   152521 cgagattaga attgggaata tgaaataaga gtggaatgtt catagcgtgt acgaaatcgt
   152581 actcacaagc gagaagctca ctcaatatat ctttgttttc tttgttgaaa ggatatagcc
   152641 ctgcgtggtc tccataaaga gcaatgagtg agctgtcgta aaggccggcc tcttttagtc
   152701 cttcaatgaa ttcgcccaat gcagcatcgg tatagtgtac cgattgaatg tagtttccga
   152761 aaagagtgcc ctcatgttct ggaaggagtt caattgctct cagttcctca ggtatttcat
   152821 agggattatg gcttgtaagc gttacaatga acgcgaagaa aggctcttcg attcctctta
   152881 ggtgtcccag tgtctgtttg aaaagagaag aatcgctcaa accaaaacca ataatctcat
   152941 cgttttccat atcctcgaga cttatgaaat catcaaatcc caatgaagga taaatcttct
   153001 cgcgattcca gaaccaacca ttattgccgt gaaaggcaac cggaaaataa cctttggatt
   153061 tcagaagcga cggcaaagcg taaaagctat tctcttcccg agtctgataa actgaaacat
   153121 caccaagtgc atgcattgaa ttgagaatta caaattcgca atcagaggtg tttcctgctc
   153181 caacctgttg atagatttca tcgaaataga tcgaatcact cgaaaccaac cgattaagat
   153241 taggtgttat ctcctggtcg ttgtaaacga agttgactag aaagtcctga aacgattcga
   153301 gctgaatgac aatgatgttt ctatctttcg caacggaaaa atatttaccc cgctcagcca
   153361 ttttattcac gtagccacta taagagactt tttcgtcaac agttatcccc ccatcgactc
   153421 ccggaatcgc cctaacaaga tcaaacatat ggtaggcaaa gactccatat ctgttgaaga
   153481 caaagtaaga ttggaggctt tccgaagaaa atggtattaa aaggctaata agtaagacaa
   153541 acgtaataat tctgtccgaa taggtcggtt tccttatggg tgtttcgccc tgtctagact
   153601 gcttctttct caagtatagt ccaacaggta tgagatcgat gataaagaca aaacttattg
   153661 gataaagaac atagactatg ctttcactta ttgcaccgac gtgtccgaca tgcacaagct
   153721 ccttaaccga tgggagaaaa ccgaagtact gaaagtataa aaaatctaca aagagtatgc
   153781 atgaaataat gcaatataag agaaaaaact tgcctttgaa tacaatccag aacaagagca
   153841 gcaggaatgc catccaggca acagtcccaa gcaacactga cagccttgca agctcagcgg
   153901 cgtagccgta aaatacgaaa gactttagca gaatcaaaac gacgattatg gtgatacttt
   153961 tcgttgtcca ttgataggtc tttttcacaa tgatctcatc ctaaacattt atacgagcaa
   154021 ctaaattata ctaactcaaa acgtatcgtt caaccagagg ttttcccttt ttggtacagc
   154081 gttgtactcc tcagaactag atagaaggaa gagcaaatgt ataactagag cgacttatat
   154141 gttgatatgt cgactctgtg attccttttc tgaagccaga gagaccaaga ggatagaacc
   154201 tgttctccga atgaggaatg gagaaattac gcagaagaaa tttaagaaat taaggaactc
   154261 aaccgcttgt tccatggtct tgatgaatag gatctcgatc ttgaacgact ctcgtcgatc
   154321 ctcggcgtgg gaatcgtttt aactcatcta tagatcgtgg gatggagagg cttgtaaatt
   154381 tcgctttaag gcagcaaaat tctgagaaag aacgtaatta cgtgggtcat gccgaaagga
   154441 gcggagattc ttcggctaag atcgtattta gtggatctgg aaagacattt ggcgaaagat
   154501 agacatcgca acttcttcct ttcaaatgtt atggcaatta ctgaaaactc acctgtcggc
   154561 ctttgcttcg aagacaatta ttcgggttcc gtgttcctca atagaggagc cagggaatcg
   154621 gggttgagag tttccgattc cgtctcttac catgggggag agagctttgt tgagtttgtg
   154681 aagtctgaga atttcgagag atgtttcagg agtatggtta tcgattacga cgagaaggtt
   154741 tcaggtttgg atgtgcgaaa ttctttgttg aaataacgga ttgaagataa cgaataaaga
   154801 actttaggag agcaggaaaa agattagaaa actccaataa gtcgctcttg atctttcaaa
   154861 ggctgcaagt gaagaacatg tctgtgatct gatcgtggtg accaagaaaa ttctggagtt
   154921 cgatatgccc ggtcttgata ttgtagagct tggaagcctt gttgttaaga gagaaaccga
   154981 gccagtttct gacaacggtg aagtccgtta cagaaaagag tagggagaag gcggcaggac
   155041 tttatgagaa gggaagacgc cagtctttcc agatacttcg aagggcaggg aggaaaaacc
   155101 gagaagtagc gaatacagat cggctctcag caaatctgtt ggaagatacg ggggtcttcc
   155161 aaacgatatc ttcagggccg gatgaattta gtagtgaagc cattgagctg gcggaagttc
   155221 ttatagtcca tgctgcagag gcaatcaaca gaataagaaa catattcgag atgacaaggc
   155281 caacctttaa cgaccctctc acgggagcac tcggcaggta tggattaaag aagttttcga
   155341 gagcagaaac cgagagagcg aaaagaaacc tctcagattt taattgataa tgatggatgt
   155401 agacggtttt aagaagttaa atgactcttc cggtcgtgtc tatggtgact ctgtgctttc
   155461 ttagcttgct gaatctgtta agaagatcat tagaagtggt gaacgaatag tcaggtgggg
   155521 aggcgttgaa ttcttaatta ttcttaccgg agtatccctc gaagatgcca ggtctgtggg
   155581 ggaaagagta cttgatcagc cgagagaaat ctgccaagaa aaaccgtaaa cagcggtgag
   155641 cataggaata acacgtttgc cagaaaacga cgatagtgtc gacgaaatga tacaccgagc
   155701 cgacagtgtg ctgtgcgcgg caaagaaaag cggcagaaac tgtgtcgata tctttcctaa
   155761 atagcaggaa cggtttttac aattgtaaat ttacccgtct gatctctttc caaatagaca
   155821 gagagccgta tgtcttttat ccagctacct tctcttgaac ccaaagaatt ccataagcag
   155881 ctatcgatat cggttggctt aaccgagatg gaagcaagca gaactgtata gttgtctgtc
   155941 tcctctaaaa ctattacgtc ctcaatcaaa tatccggtaa gccgtgactc ttctggaagc
   156001 caacgatctt caaagccttt caagtaatca cctagcaagg agctaccgat ttcctttgca
   156061 gtaatctctt taacaaactc gctttcaaca gaagaatgaa acaaagtgtc cccaaggaaa
   156121 agcaccgtgc cgagcaaggc tgcttgcaga agaatggcca ggtagatcca tctgtaatct
   156181 tccattagaa tcaactccca tataccatga aaaaagacct cacgcagatt ataccgtatt
   156241 tcttcggcat atcagaactt ccgtgaacaa ttggaaagcg aatagagtgg tcgacagaac
   156301 gtggaatcgc accgctagat taatctaatt tttacgctct caataaagac aaatcgtcag
   156361 ttttgaaaag gaaagctctt gcctcgcttt aaagacaaga gccatcttta gtaagtctca
   156421 tttcatacta ggacagggat ttcaggacct gtctggccca gtttgcactt gcgttggctg
   156481 catattttac gccggaacct attggatcgt agaaatctat ggcaccgaca attttgtttt
   156541 ccgaaagctt gcttatcaac tcctggaaaa ctattcccat taggccctca tgggtacaaa
   156601 atagagcgat gtttttattg gaaagtttgg tttttctaat gaagctttcg attggcggtg
   156661 cgtagctcat tgcccaaacc ggtgaaccaa taaaaattag gtcgtaattg tcggggtcaa
   156721 gcaaaaaagg ttcaagatcc ggcacggggt ccatactaac actctcccct tcccaaatgt
   156781 acttcacttt tcttccgcca cttttttcca ccggttttag ctcgagaaga tcggcattgg
   156841 tttcgcctgc aattgattcg gcaattgatt tagtactttc gtcatgagag taataaacaa
   156901 ctagaacgtt ccttccattt tctacagaca taaatcagac ctccaattaa tatccgacta
   156961 ttattatcta tatttattct ctcacatttg acgttgtttt catagaacta taaggtgtct
   157021 tcgactaatc ttgagcaaaa cgcatttctg cgtactttgt cttgaatccc atctttttat
   157081 agagaaacct tgcagggtta ttctcttcaa catgcagggc aatatcgccg ttgcacattc
   157141 tctttatctg tttgatcagt tttttaccga tgcctatgcc tctggcttct ctgtcaaccg
   157201 cgatgtaaac gagtatgtgt tctggtatat accccttcat ccccgtgtcg tttactacag
   157261 aagcaccaag aatcctctct ccatgtcttg caataagaac gaagccacct ttacccgctt
   157321 cgttcgagaa ggcatattca atggatctcc agattgcttt tctatcgtca ccgtactctt
   157381 caaggtgagt atgaaggaag tcgacgattc tgtcgattcc gatttccttg tcggcagttt
   157441 ccatgttctc aaatcgatca atagtgatct tctcgttttt ggttagcagc ttcaaaaaaa
   157501 cacctccgtt ttgaaataga tattcgtttg tatatctaat ataattatac catcgtgaat
   157561 tgttaacaac tgtaaaagct tattatagcg atctcaacta tttcggctga aaagggatta
   157621 cgggaggtgg cttaacaaaa tctttgcctt cattagaaac aagccagtac aaactaccgt
   157681 cggacttccc cactctcaag ctctattcaa tgagatatct tcccaaaaga actaatcatc
   157741 gcagaatctc tttaacttct cgtttacctc ttcattgttg tttctcagca ctggctcaca
   157801 aacacctgcg atctcccatg gcactattcc tgtccttctg cctattccta agtctgatca
   157861 actcaccgga ctcactgaag ttttcgcgag ccgctacgct cgttcgcgct cagtaacagc
   157921 ttactgctca tctaccgtag ctgcgtttcc acgtacatct actattctgt cttccttaat
   157981 tacgctgggt tccatcatct cgtgaaaaag aagaaaggtc tttgcctgtc tagccttctc
   158041 tctcagtcct gatctatgat ttctcacgtg tcgttgactc aagttcagtt tttttgagtt
   158101 tatttgttgc ccagtgatgt gcaagaatac gctatcattg tctgctggtg ttctgagagt
   158161 ccggtgtagt tcctttcatg catgagaata gagtgaaagg gcgaaacacg gtttctagat
   158221 ctgtgttctt tcgctgccat ttcggcgtct ataagaactt catcgggatt tccaaagacc
   158281 tctcccttct taaaagacat ttcacatgtc aaacagacat agtctgtgtt tagaactcta
   158341 taagcggcct tcaattcctc aatgctgctt tccaagaaaa gttcggaaga ttcaatgaac
   158401 taacctctcg cttgagagta tgtatgatag ataatgatga agagtgcaaa caagagaggt
   158461 atgtgtttga tcataagtaa ctatgcagtt cactcgttta tcacaatacc tcgacgcctt
   158521 taagccttca gacaatggta ggtgaacagg agttgaaaac tgttgttcat atttccagtg
   158581 cgagaactct tcttttcagc cgaactttga atccggcttt ttcatagatc ttctttgccg
   158641 aggttctgtc agacgtcacc gatagaattg cccgtgtctt gcccagtttc ttaaggtatg
   158701 taagagaagt cgccaagagg gctcttgcca cgccctttcc tctccactcc tgtaccacaa
   158761 agatttcctc agtgtagcac ttagcactgt tttgagcatc gggataaacc attacacttc
   158821 ctgcaatgtt tcctccggca tcgatggcgg caattattac gccttctcgt ctccattcgt
   158881 ctctactgaa gaagtaattg agatacttgt attctagtgg ggaatcggga aagcatatct
   158941 gctcaaccct gagatattgc aaaatctccg gctctcttct gggtttcagc tctgcgatat
   159001 ttaaaccctc aggaaggcta taagaagaga tctcttcact gagatccttc tccatgtaaa
   159061 taaagtcgat ccagtgagtg aatcctgctg aggaaaagaa atcactctta gtcttctcgg
   159121 tgtcggcgac tttcacacat attcgcctcc cggcggacgg aaagaagccc ctcgtggatt
   159181 cgataatcaa aggaatcgaa gctttgtaaa gggcagacat tagatccgga tctgcgaagt
   159241 gctcaagtat tatttcgagc caaatgtgat gaaagaactc tcttgagaaa ttctgccggg
   159301 agaagaatct tgaaaatccc agaacccttc cattatcgac tgccagaaga atattctttc
   159361 ttccgttcag caaaggattt ctgaaatcgt cagccgtgaa tttctcggcg tccggatggt
   159421 ctctttcata atctaactga atgtctaaaa ttctttgctt aaaatccttt gaatagccct
   159481 gaattatcac ttatgaacct ccgcttgaat gatctcaaaa tggataaagt gccagctttc
   159541 atacttatta acaatcatca ctaatcaaat atcatcatct tatcaagtta ccccggataa
   159601 ctctgagata aaagtggcat aatatataaa taatagaatc tactagagtt tgtcaagcgt
   159661 tgcacggaaa atcaagttct tagggcgtga tgaagttatg gaaaacagag actctagtgg
   159721 atttgcaagt atcttgaagt tcggaggtgg accactaatg ttttttggta agaaagaagt
   159781 ggcgattatt cagcttttcc acgagcacct ggatttcatc tcaaagactc tggagaacct
   159841 tgaaaaggtc tttcttgcca ttgagagtga gaattctatg tcgatagaga tttacgcgga
   159901 agaggtagga aaaatggaat cggctgcaga tgcaaagagg cgcgagatgg aaaatgccat
   159961 gtatcaaggg gccttccttc ccaattttcg cggagatttt ctcggccttg cagaaacgtt
   160021 tgataaggta gctaatgaag cggaaaatgt tgttgaccag ataatactgc aacatctctc
   160081 ggtacctcaa gagctcagga atgatctgct aaaacaggct cagctggcaa ttaaaactta
   160141 tgaagtatcg aaggaggcag ctctaaacct ctttcaggag ctcagtgtag ccgaggagat
   160201 gataaaggaa acggaacgtc tcgagaacat tgaggacagc cacgagcggg cacttataaa
   160261 aaggattttt gaaatggatc tcacacttgc cgagaagaga cagctgcggg aactggttct
   160321 ttcgattggt gatattgcta attattcgga ggattgctcc gaccgtatgg aaatcatcgt
   160381 cctaaaaaga agagtttagt cagaaaagtc ccttaacggc attgacaacc aaataaagcc
   160441 caagggatac gaatccggcg atcaacggag tttgtaacca tcctagagct atattccgta
   160501 ggaccttaaa gttgatgtcg ctggaacctc ttgcaaggcc tgcaccaatt actgcaccaa
   160561 ctattgcctg agaagttgaa actggaactc ctattactgc aaaaacccac acagttatcg
   160621 cctgggaaag cacggctatc acagcggaga aatgatcgag cagcgtaatc tgctttccga
   160681 cagtgagcat tactctcttg ctgaatgtca ggacgccaag ggcaatactt agtccaccga
   160741 ccagcgcggc gatatcgtat gaaattatgc ctgcaagcgg gccggtgacg ttagcaacgt
   160801 tgtttgcacc aagagcatat gagccatacg cacctataat cagtgctcca atcttaagtg
   160861 aaagatcttg agcttgtatt gtctttattc ttctgaatag aatcgcagat agcttgtaga
   160921 gaaagaatga accaatggct gcccctatag gggtggctat ccagcagatt actactttaa
   160981 ttagaaccga ccagtcaact gtcgagttta ggatccctat ccccagaatt gctcccatca
   161041 ttgcttggga ggaagaggaa gggattccca gatatgtcat tattgtcatt gaaatagccg
   161101 ctccaattgt gcttacagaa gcagtaagaa gtgtctgact gctaacactg gagattgttt
   161161 ccagtcccct ggaaccctcc aggagcgcac caataactat gaagacagca gagagcacca
   161221 ccgcgacgcg gtattttacg agacctgaag taaccgcagt tccatataca tttgcagcgt
   161281 cattcgcacc caaagcccag ccgaaaacaa cagcaggtat tatcgcaaaa agcataatag
   161341 cctccactgg agaatggaaa tttgattgat tatatcactg ccgttattag attcgaagcc
   161401 acttttgggg cttgattaaa gatcaaagtc ggcagaaata cggatatgga agttttttgg
   161461 agctggcaac actaaaactc aatgaagaca atctccggta gacttgcgag tctgccggga
   161521 aaccagcctc cgactcccct agttacgaag acctttgtcc cattgatctg ataaagaccc
   161581 ctgaagaaat ctccgctgaa gccccttgta ctcctaagaa tggcttcagt taaccagggg
   161641 atgtagaact gaccgccgtg agtatgccct gcaagaacga ggtcaaaatc cttcaatacg
   161701 ttttcgtgtt cttcaagaag atttggagcg tgaaccatca gtatcttgag atcgtagtca
   161761 tcttccggaa gaaactttgt ccatgagaag tagtgatgtg aaggagttcc agtaagtccc
   161821 agtttcttat ctgagatctg aatttcagtt gagtttgcat ggacaacatt tattcctgct
   161881 tcctcataga ttttctgcat cgtagtcgtt tccgactctt cccagttgcc gtaaactgca
   161941 tatacgggcg caatcgcagt cagtttggat agaaagctct ccatgtattc catatccgtg
   162001 taaggagcca gaacatctcc gccaaaaaga atcaaatcga ccccttcatc tgtgaatttc
   162061 tctagaaggc gattatgcag cggcttcttc gtccatatat gaatatcggc gaaaaaaact
   162121 attttcgccg agggttcatc ggaaaaagta agcctctcaa ccgtcaaaat gttaggctcg
   162181 ataagaaaca cataggcaac aacgcaagca aaaatcaacc agaatttcca atgcttcaag
   162241 agaaaccttc cccaaagagt tttgtcgtgc gttaacattt tacattattg ttgggccgta
   162301 ccgatctgtt tcactaaata aatcgtgatt tcttctttac aagtgcccta cctttccatt
   162361 tcgtgcatcc aaagaagtga aagataatcg acatatagcc aatgatggat gagatcgttg
   162421 cgataagcgg atacaaaacg acaactaaag gcgaaagacc gaacttctca cttgcgagaa
   162481 accagaagat tattgaaaac aacacgctaa cagttgagga aatagcaatt gtaggtgaag
   162541 taaccgtacc ggaaatggac agaattaaca ttaccaaagg gaaaaggttt atcgtaagta
   162601 tccatgtcca cacaaaaatc gccggaatca agcgatattc gaatagggca aagtagtttt
   162661 tgagaaaacc cgcgcaggca tctttgaaac tctcatacat tttgcactca acaaggtctg
   162721 aggcattgta aagcctccac ctcattcctg ccttcttaat cagctttcca agagaaaggt
   162781 catctactcc atggtctcta acggactcat gtccaccaat agaacaatat gactccttct
   162841 tgaacatcat gaactgccca caggtgacag aaagtgctcg aaacttggtt ctgtaggcta
   162901 aagccaaggg gaaaatcgac aatatgctgt gcaccatgaa gggcaccgtc aacaattctc
   162961 ccaaggtatc ggttctttct ttgactaccg ccgaaagcaa atctagatct tctatctgca
   163021 ttgctttgac tgcaaaggag acagtcgacg gcgagtgcac agtatccgca tctgtaaaaa
   163081 ataagatctc cccctttgct tccattgaaa gttgattgca tgcccagtgt ttaccaagcc
   163141 aacccttggg caacgccttc cctttgatta ttcttaaatt gtcggaattc tttgccagtg
   163201 attgcaggat ttccatcgtc ctgtcggaag agcagtcttc aagcactatt acctcgatat
   163261 tgctgtagtc ctggttaagg agagacgtaa cacatgcaaa gatattcttc tcctcatttc
   163321 tggcggggat caatatggaa accattggtc caggaatagc tttctttaag cgactcattc
   163381 tcttaatcac gaaaagattt gaaagagtaa taactagcat gattccgaga aagacagaaa
   163441 tcgagataga ctgagattga agaaagccca cctcacttac ctccaaaaaa cttcagcttt
   163501 attggaaaga ccaactgatc tctgtgcttg aaggcaagta gagaagtatt gagtatccat
   163561 gtcagggtaa gaaccaaatc tctttgaaca agtagaagtg caagaaggcc aaacatgctt
   163621 agaatgacct tccaaccatc tgtactgggt accagaataa acgtaaaaaa caacattaac
   163681 acaccgatta tcgtcggccc gacccacaag gtcaatcctg accaaatacc aaaagaaact
   163741 gcgactgcct ttcctccttt cattcccagg aaaggggaaa aggcgtgtcc caagacgggc
   163801 gaaatcgcaa cagggacaag tcctaggcct gaaatatccc caacagtacg cgctagcaat
   163861 acaggaagat accctttaga atagtcaagt gccatcgcaa tcaaccccag aataatgcct
   163921 gcgcctctaa ttacatttga tgaaccggga ttgccgtcgg cgatttccga tattctctta
   163981 ccggtagttt tctcatagag gatttttgag tacattaccg atccggaaag aaagcccaaa
   164041 gctgcccaca aaagaaacat gatccaatcc tcctatgtaa aaacgcaaga ctcccctttc
   164101 cgttcgccct cactggatat tatgcaaagc tgaccggtac cgaagcttca tattaggatt
   164161 atacagaatc tccactccat aattcgacag taaaattcat ctttggtggt accgacagaa
   164221 cttcagtcga tggtacaatc tcatcgaaac ttgaaaggat ggtgaagatg aagcgaatcc
   164281 agtttatagg tatcattttg atcatgtgga cgaccgcatt ttcagtacag aatctcgctg
   164341 atctctcttc cgaagactgg agagaggata tcgagtacct tgaagaaagg ctgataagca
   164401 ttcatcccaa tcccttcttc aggatagatc gggaggtctt tcaagcttcg attaaagccc
   164461 ttgagcaagg tcttgatgag ttgtcatggt atgaaattta cacaagactg acggaaatcg
   164521 ttgcgtcgat tggagatgga catacggttg tctatcctca aggcagttcc acgctctatc
   164581 cgatatatac ttactggttc agtgacggtc tctacgtaat tgctacatcg gagagtgaac
   164641 agagacttct taattcgaaa gtcctcgaaa tatccggcac gaaagttgat gatgctttga
   164701 aagcccttag aagagttgtg tctcacgaca acgaatgggg tttcatgaat tctctttctg
   164761 actatttgaa caaacccgag atcatgatgg gtcttggact tgccgacaaa gaagggaatc
   164821 tggttcttaa gatcgaaaac aacggagctg tcagtgaagt gctcgttaaa cctcagaatg
   164881 ccgggttcca atgggttcag aagagaatcg aagaaatcga cagaacttat attggaagaa
   164941 agtactggta tcagtactac cctgattcaa gaatactgca ctttcattac gcgtcatgtg
   165001 gctcggaaga aaggaacccg ttcattctat tcaactgcag aatgtttcta tttacctgga
   165061 cgaacccagt cgacaagttc gttcttgatc ttagaggaaa cggaggaggt agctcagtca
   165121 ttctggaacc tttcattcta agaatgatga tagattggag gctgaacaga acgggaaagt
   165181 tttttgttct aatcgatcgt ggaaccttct cttcggcagt tctcaatgcc atttctctga
   165241 aaaagcgcac aaatgcgatc tttgtgggag aaccaacggg aggcagtccg aggcattatg
   165301 gtgaagtgaa gagattcgtc ttacccaact ccggaatatc tgtaagttgt tcaagcactt
   165361 actggagaac aacttccgat acatcaccgg ctttcatgcc agatgtcatt gctattagat
   165421 cttttgagga ctactactat atgcgagatc tggcaatgga ggcagttgaa tcctatagaa
   165481 ccgaaaagga ggaataaatg atcacgctgt tcaagaacgt tgaagtattc acaccgaagc
   165541 ctcttggaaa gaaggatatt cttgtttcgg gtgaaaggat catagagata gatgagcgaa
   165601 ttgatgaatc tgcagtagtc tccttaaatg gaagagttct cgatctttcc gatagatacg
   165661 cagttcccgg atttgttgat ggccacgtcc atctcatcgg tggaggagga gaaggaggct
   165721 tttctaccag aactccagaa ggtagctcga agcagtttct cgaagtcggc actactacca
   165781 tagtcggcct attaggtaca gacggtatta cgagagacca catttctctc cttgcaaagg
   165841 ccagagacgt tcaaaacgat ggactgaacg cgttcatgct taccggttca tacaggtatc
   165901 cgttaaagac gattaccgga gacattatga gggacatagt cctgataccg gaaatcattg
   165961 gcgttggcga agtcgcgatc tccgatcaca gaagcagtaa tctgagcgcc aaagaattac
   166021 agagatttgc aatggatgct cgagttgccg gcatgctctc aggcaaggca ggagttgttg
   166081 ttctacatct tggagacggt caggagaagt tgaagcctct ttatgaagct acttcagatg
   166141 gtaccctcag tccaactcag ttgattccaa ctcatatctg tcgtactgaa gagctccttt
   166201 ctcagggcat tgactgggtg agcaacaagg gtggctatat agacataact gcaaatgagg
   166261 ataacacaca cctggttcta agcgatattt acagaagaaa gataagattc gacagaattt
   166321 gcgtatcatc tgacggactt ggttcacttc caaagttcga cagcgagaag aacctcgtgg
   166381 gaataaaacc agcaccagtc gacactctta tgaaaagctt cataaagctt ataagtgaag
   166441 aaggacttcc aattgatgag gctctcagac ccttcacgat taatccttcc aggttcttcc
   166501 atcttgaaga aaacgggata ggaacactga agaagggcgg catcgccagt attgtttttc
   166561 ttcacagaga tcttagcatc gaccatgttc tttctaacgg ccggttgtta gtgtagaagg
   166621 aggctcatca gaatggaatt tgccgcagtg tattacagta gatcgggttc caccagagct
   166681 atcgcagaaa gctttgcaag ttcttgtggt gcaaagctct ttaaactaga ggatctgaaa
   166741 cccaggaaat ctgtttcggg gttctccgct cttctgggtt taggaagcgc tttgaaagag
   166801 ccacttccgg atgtaagaag tttcgacttg gttgttcttt tcactccgat ttttgcgtgg
   166861 catccttcac ctcagatgaa tacattcgta aggaatggag atttgaaagg taagcatgtc
   166921 tatcttgtcg gagttggagc cggtgagagc aatgttaaag ctctagagag gttcaagaaa
   166981 gaagttgaaa gggccggagc taaagttttg ggttcaaaga acctaaaagg acttcaaatt
   167041 aagcagaata taaaagaggt agagccgcaa cttttggaga gtggcaatca gctggttggc
   167101 gccattgaga aggctttcaa agagacctag aacagatgct ccagaagtat cttggcgccg
   167161 atcgctatca gcactgttcc cccggctgcc tccatagctc taccgagttt ctggccgatc
   167221 ctttgacctc ctgttactcc gattgccgaa agcgaagcgg tgatcactcc agtcattata
   167281 gcagtctcta taactggctt ttctaagaat gcataggcac tacctacggc taatgcatct
   167341 atcgatgtgg cgactgagag aagaatcaga gtcagccctt tggttcgatc cttgcaagtt
   167401 tcctctccct ggttttcaaa agactcgtag agcatttccc ctccaattgc tgctagcaga
   167461 atgaatgcta tccagtgatc aaaatccaca atgaagtctc tgaagcctgc tgccaggaag
   167521 actccaatga gcggcatacc aaactggaag aacccgaaat ggaaagatac cctgaaaaca
   167581 tgccttcctg tcacgttagg aagagagatt cctgtacaaa tcgaaacggc gaatgcatct
   167641 aatgcaagac cgtagcaact aacagattct ccatccaaag ctcctgtagt aagattaact
   167701 agatagtagc atatagtcac cagaaaaatc tgtgaagcgg attaggtgac ttgtcgactc
   167761 tgatcgtatc ttttgagggc tccagtaatc tcttaaacag aaaatcccat ccaaagactt
   167821 tcaagatgtg gagagcgatt tgacgacgcc taatctggag gttggaactg ggagggggag
   167881 agggtgttat cataagaagt aatgcgaggc tatcaaggtg gtgcatcttt ggagataatc
   167941 tacggcacac gaaatctggc caaagtgatg agcatgaaga agatgctttg cggattggat
   168001 ctgcaattaa aggatttatc tttttttgag catcttccgg ttgtcgaaga gatcggatca
   168061 agtccgctcg acaatgcacg agcaaaggcg gaaacttact atcgtttttt cgcaagccct
   168121 gtatttgcat gtgattctgg tctgtacatc gaaggcctag attctggtct tcagcctgga
   168181 gcaaaagtaa ggagaaaagg ttctaaggtt ctaaatgatg atgaaatgat cgactactac
   168241 aaaaagcttt tggaaggact tggagggcaa gcagtggcac gttatagaaa cgcaatcttt
   168301 cttatctttg acgatgatca gaaatgctgg ctgatggacg acagaataga agagacgttt
   168361 taccttgtaa gtagacctca cccgtcaaga gtagaaggtt ttcctctgga ttcactctct
   168421 atagatatta ggtcgggtaa gtactttgtt gatcttaccg acgaagaaag gtccagtgct
   168481 tatcactgtc gtgatggctt caggaatttc tttagtgaag caatcagttc atttagtgat
   168541 cggcatttct gatcggaaat atcgtaatcc catagttcat ggcatgtcat taggcgacga
   168601 aatatttagg ggaggataaa atggcttata gaacaatcaa cggagttaag ctttattatg
   168661 agatcagagg caatgaagaa ggggaagaag ccgtcgcctt tttcaatgga gtcatggctt
   168721 cagtaaacag ctggtcgctt caggcacgtg tgtttgagag atttggctac aaggttcttc
   168781 tccacgattt caaggggcag cttctttcag agaaaccgga aggtccctac acattcaaag
   168841 aacatgcgga agaggcaaga cttctcatga aggaactggg aatcgaaaag gtgcatttga
   168901 taggcacttc atacggtgga gaagtggcct taagatttgc tatagatttt ccggacacag
   168961 tcaaatcgat ttcaataatc gattcggtaa gcgagctcga cgaagttctc aagcttttta
   169021 ttcaaggctg gaaggatgca gccagagctg gagatccaag gaagttcttc tgggacatgg
   169081 taccgactat ctatggtgat gctttcatta gaaagaacct taaaatgctt gaagagagag
   169141 ctatggccct tgaaaaagtc agttccgatt actttgaagg gcagattgcc ctatacgaga
   169201 ctttcttagg ggacgtttac atgaccgacc ttcttgaaag aattagctgt cggacactcg
   169261 tagtatgcgg ggaagaggat atacttaagc ccagaaagtt ctcaaagatc atagcagatc
   169321 gaatccagaa gtcagagttt gcaataattc ctgatagtgg tcatgtgaca atattcgaaa
   169381 agcctgatgt gcttaacagc ctgctcctcg gatttgtcat gaagagttcc gatctatatg
   169441 cacaataggc cacgggattt tgctcgtaga tcttacatta gacctgacat cattagtgtc
   169501 acgaaatgag tttgttgttg ttcgttgcca atatagatta acaggtacta ctttcacacc
   169561 agttgtcggc aatgcatctt tcgcttatac gggcagcttc agagttgctg aaagccgttt
   169621 ctgggatgcg tctcttcttc atggatctcg aagcaaggat catcttctga aactacagta
   169681 gaatcaaaag gatagcgaca aagatactac aagtattttt tgtcgctacc tttttttgtg
   169741 aacaagtcca atatggtctt ccgaaaagcc gagaattagt tcatattgcc ctattggatt
   169801 ttgaaatact tgatccaagg tctgcaatat ctagaagtct ctttgaaccc aaatctcctg
   169861 taaaaaccaa cggtggagtt catgaaggag cgatctattc caaatgattt cattcttcgg
   169921 aagccctcct taagcaactc tgttgccaac ccaagctttc tatatgaaat gtgagtccct
   169981 acgggttcga agtttccgta gccgtttttc aagtcaagcc agatagtgca gaatgatgcg
   170041 tattcaccgt ccggagcaac tacgaaaagg tcgagttctt ttctgtaatc aggtgccatc
   170101 tgcatttctc tgaatgcatc gaccggtggc cagctcgagg gtgggcagta acccgagaag
   170161 gccatagcct ttgctcttga tctcttgact ggatcgtctt cctcggcgac actgagaact
   170221 ctgaaccctt cgggaaagga aggggttgcc attgactcgc cgatctcata aaccaagtgg
   170281 ggaatacatc tttcctgcct tacatatcct ctgctcttcg caatcttttc aagctcgtcg
   170341 ccgtcattta cataaagctt tacaaaacca attccatttg ctctgtctgc aagattctct
   170401 tcgatgtaat cgacgatttc aggataaaga tatttgtaag cggggtgtat ctgaatccat
   170461 gcttcaccgg gctcctcgtt ttctgaatga gcaacagcaa caattctgcc ttcttcgttt
   170521 tcccatattc cgattgaatc ctgccaaaga ggaagatcgt ctctatgatc tatgtgaggt
   170581 cttgtaggga cactgaaata gctcgtagaa taatatctgt gaagaggtag gacatgaaat
   170641 ctgcaaaaat tccatctctc aatcagccag ttgaacggac ccccatgaag aggaaatgtt
   170701 tcgatgatca actctctaat ttgcatatag tctctctccg gcacgtacct tctgagagtc
   170761 acaatcttgc tcaacgtgat ccctccttaa attaagaaag agtagacata atgacggaat
   170821 tatatcatca ggagtttaga cacacttggt cttcttttca taatgtgccc acacgaaagt
   170881 gaccgggctt catccccggc cactcctctt aagaaaaaca caagtatgtg ctttcagtaa
   170941 gactcaatat tgaattgctc ttggaagttt ctttgcctgt tctatccaat tgagtatctg
   171001 ctttttcgtt ggaagctttc gaacaagctt cttttcacca ttaacttcaa cgatcttttg
   171061 gaaaaggttg tcaccgtttc tctttgaaag ctcgggaact gtatcaactc ctgcagcctc
   171121 aagaagctcg gagtattccc ccccgattcc tttgattctc ataagatcag cgtggtttac
   171181 ccaggtaagg atattgtcct cactaatgtc cgccttcttc gccagttcgg tccttgcttt
   171241 ctttgtctca caggtcttta gcaaagattc gattgaagta attccagctt ccttcagttt
   171301 tttctcgtag accggtccga ttccttcaac gatagtaagt tttgccattc aaattcctcc
   171361 cttctattag tggtgttttc tgaattcctt gttgcaaatt gtgggatagc caaaagtaac
   171421 agtctaacta tttcttaaag aactttccaa agaaactacc ggcgtcatcg agaaaactgc
   171481 cgtccctatc cttgtccagc aaatcagccg cttttcccat catgccgctg ttactgctac
   171541 tggatgcaat attgctgaga aacccggcaa ttcccgatga atcaacatta cttttcgtct
   171601 tctcatttcc aagtgcggcc aatagcaaag gtgcaatctg tgaaagaatt cctgaaacct
   171661 ggttcttctt taatccggtt tgagttgcaa ggttcttctc aactctctct tcgttttctg
   171721 gaaaaatgtg gctaagaatc ttctgacctc cgcttcgatc agcatttgaa aggtatgact
   171781 ttacgtcatc gacattctca tccttatgtt gatcaagcgc tctgttaagc gattcggctc
   171841 cctcttgggt agaggcgttt cgcgttagcc cttgcagaag cgtcggcagt ccgagttgag
   171901 ttaccttctg taccttatct ggagtagttc caacagattt cgcgagttct gaagatgtat
   171961 cttcatcatt gagtttttgt gagatcagat cgaataaatt tattggtctc ctcctttgaa
   172021 cattctgtaa catctacttc attatacttc ttaatctcaa aaagtaagat cataagcgag
   172081 actcaatcta atgagcgaaa tatctatctt catttgctgt ttcgtttaaa tcttttcgat
   172141 ccgaaagttg caatcatttg tgaagcggtg gttcaggaag aaagagatcg tattctgaac
   172201 gcccacctgg cttcgaataa tgtaaatcag gaaaaaccgt agaagaaaat cgcactccga
   172261 acatagtggg cctgtattga agaagagtgt aaaaagcaaa tttcaggtgt gccttattat
   172321 tcgattcctt tttgcaggtc gttctggagc tattacgttc cagaaagtct tttggtgatt
   172381 ttagcttgtt cacttatcac acattgcaga ctggcctgat cgtttcgata gttgcgtttc
   172441 tgaatattgt atctgactgg aaaggctgga aatcacacaa agtcggcgaa gtattgcgaa
   172501 tcctaaacgg tggttccgtt ctctgggtaa ccgttacatg tgtatttacc tcctcctatc
   172561 cggttacatc acccgaaggg agtggctcta aatgcaaaca ctctcgttcg ctgcgttact
   172621 ccactcggta gaatcataaa ctgggtgcta atttaggaga gatgctccaa taagtttttc
   172681 taccccctgt tgcaacttct tcaaccttct ggatacgcag ccgtgcccgt cggtcgcaat
   172741 tcattaagcg gtttctatcc atactggttc ttgaatccat ctgcagaata ccatgcgagg
   172801 attggttcat acaccaatct aatgatcttc atcctgccag tggggacgat ctttgtagca
   172861 tttgattttg cgattttgat tcatttttcg ccgaggaaca agttgatgta aatcatttac
   172921 attgggctag aacatgaaat cacttccccg tgaatatcaa tcagtacttg cgctacctga
   172981 tatagaaaaa gaggggcttc gccctccttc tcaaaactga actccagcct cagcgattgg
   173041 cgaagtttct ttgatgaccc ttgtctttgt cggacgattc atcctcttga gattgccgtc
   173101 tctcagacca tgccaaagcg tctgcccttc gtttggaaac aactcattca ctctgtctcc
   173161 atcttcatca acaaaatccg atgcactctt taagagatag gctgaccgtt cctctttcct
   173221 ctgaactgct gccctacgta cgtaggtaag agtcgatgcc aagagaatct caagttcggg
   173281 tggaacagtt ttctttatcc ctgtgctccc ccctattcaa atagtgaatt atctttcaac
   173341 ccaaactctg gaggtcccct ggactccagc tatggccatt aagaaaggaa atctgcaacc
   173401 tcaactatcc cagatgcttg taaggagtcg actcaaagag aaagaatttc tcatggattc
   173461 aaactcttct tgtagatcag cagctttgcg gaagatttcc aacaacttct ctgatttttt
   173521 cttgttcaaa gacagcaacg attcgccttt atcttgctcc gaccgtgcag agggttagct
   173581 tcgtcttata atcatttcgc cttcaggcat ccaaacgatc ttctagatcg tctctcgaga
   173641 ttatcttagg aatttgccat atcgacaact gccttcgctc agactcacca ggctttcaag
   173701 gtgaacaagc aagaaactca agaaattaag gcatggagca ctaaaagcta aaactgcgga
   173761 aatcctgctt cattcataaa aatcagttca gcacgaaggt ccagatatct tcctaacgcc
   173821 agtgaaaaac catcattcac gtcgttttct tgcatctaag aaagaaaatt gccttcattc
   173881 gatccagatt cctcctcttt catccggatc aggagtatgc gggtttaatc aaccgaataa
   173941 tcggatgtat aatgttaacg ttaactgtta acgttaacaa gcagaggagg atcgtttgtg
   174001 aaaagggcca ccttgcgaga agtagcaaaa aaagccggga catccatgat gacagtatcc
   174061 agagtgatca actccaagga ttcggtaagc gatgaaacca gaaatagagt cctaagagca
   174121 ataagtgagc tgggctatga gcctcacaaa gacgcgagaa ttctcagggg tggaaagact
   174181 ggcagaattg ggatagtggt ctccgatatc aggaacccat tctattcaca ggtggttgga
   174241 gatctcgaag accttgctga agagaacaat atggccatta ttgtttcaga cacaagcaag
   174301 aagcttaatc aggagagaaa ggctataaag tcgcttctgg acataaaggt tgatgcaatc
   174361 ctggtggctc ctgaggggta tgagagctct cacttaattg atgtgattaa ttcgggaaca
   174421 gacgtagttt ccttcggcgt tcattttgaa aacgacgaaa tttcggaggt ttccatcgat
   174481 gaaatcacag gtgcggcgaa ggcaggagct tatctcaaga gtgttggaat taaagatgtt
   174541 gtaatgatga tggggaatcc tcgcaagttt actacaagag gaagaatgga agggtttatg
   174601 agaggttttg gacacgtgcc tgaagacaga gtgatatact gtgaggttga ttggaaggca
   174661 tcttgcgcaa ctgttagtga aatgcagaag cttcctcagg ctttcttttg ctacaacgac
   174721 atgatggctc tgggagtgat gaaggcattg gaagaaaggg gaatagaact aggaagtgaa
   174781 gttaaggtta ttggctatga cgatgtttat atggccgaaa ttgctggcat aacaacttta
   174841 agaattccga ttcgtagcat ggtgaaagaa gctttcaaga caatattggg agacgaggta
   174901 agaaaaatgg tctttacacc ggaattcata cgacgcaaga gcgcgtaaac aggaggtggc
   174961 gaaagtgaaa agagttttgt tggtcattct gttcgtttcg gtggctctgg taggcttggg
   175021 gattcaacaa ctggttgtga attcctatat gtctgacccg gaaccgaaga gagtttttga
   175081 ggaacttgta caacagttcg aatcaatgta cccggacttt gaggtaaccg tgaacacgtt
   175141 tgcacatgag gatttcaaag tgcttctcag aacatggctt acttcatcaa atccgcctga
   175201 tgttgtcacc tggtttgctg gtgaaagaat gagatatttt gccagcaagg atcttctcga
   175261 accaattgat gatatctttg agaatggatt tgaagcagat ttcccaaagg catttgttag
   175321 cgcttctagt tacgaagatc gtatttattt cctgccacaa tcatggtact ggtggggtgt
   175381 gtactataga aagtccgttt tcgaagaact ggggatcacc gtacccctaa cttggaagca
   175441 atttctggac gtctgtgaga cattgaaggc aaatgacatg atccctataa ccattgggac
   175501 aaaatatcta tggactgccg ccggggtctt tgattacctg aacatgagag ttaacggaat
   175561 cgattacgct ctaaagctga caaacggaga aatcccatat actgacgatg ggatgaaggc
   175621 ggttttcgag tactggaaac agcttgtaga aaacggttat ttcattgagg atgcaacatc
   175681 ctacacatgg caggaagctg ccacctatct cttcacagga gaagcaggca tgtacctgat
   175741 ggggcagttc ataaaggatg ttgctccacc tgatgtgaag gatgatcttg acttcttcag
   175801 gttccctatt attgatggta atcttggtct ttacgaagac accccaatcg acggcttcat
   175861 gatgccggca aatgccaaga acaaagaggc aggcaagaca ttcctcaaat tcatcgcttc
   175921 gaaggaagcg caagaatact ttgccacgga acttggaaga ttggctgcca ataagcatgt
   175981 ccccgcaccg gacgaccacg ccaagaaagg tttggaaatg atactttcat ctgacggagt
   176041 gatgcagttc tacgatcggg ataccgatcc agaaatggct aatgcgggaa tgaacggctt
   176101 tgtggaattc atgaccttcc cttccagact cgatacgatt ctaaagaatc tcgaagccga
   176161 acgaaacaga atctacaaat aaggctttct gtacagggtg ggtcggcagg acccaccctt
   176221 catttggagg aaataatgag aagcaagaga tgggttccat gggctttcct tgcccttcct
   176281 cttacgatgt attctatatg ggtcatatat ccattgatca gtacgcttct tctcagcttc
   176341 accagctggg acggggtctc gatgtccaaa gatattatcg gcctggacaa cttcagagaa
   176401 ttgttcagag acccatattt cattgtttcc ttgatcaaca atattaagtg gctgattgga
   176461 tttgcggtgg tatgcgtacc tgcgggcctg gggattgctc ttcttctaga ccagaacttt
   176521 cggggaaaca agctttacaa gacgcttgtc tatcttccta tgaccctttc gttcgttgtc
   176581 attggacaga tctggtcctg gattctcgag ccaagaagcg gcgttttgaa cagtttattg
   176641 gcgttgctgg gattcaaagg cgtttcgtgg ttgagcgacc cctccgtagt aacctactct
   176701 ctgataatgg cagcgtcttg gaggcagatt tcttacgcta tggttctttt tcttgccggg
   176761 cttaagggtg ttccgaggga actagttgaa gctgccacgg ttgatggcgc tggtccttgg
   176821 aagagattct ggaacgtcgt gcttccgatg ctcaagccag cgaccgttgt ggccgttacg
   176881 gtaagcgtta tcgattctct cagggctttc gatattgtct atgttctgac acgcggcgga
   176941 cctttctact catcatctgt gatggcgaac tacatgtaca ttaaggcatt caataattac
   177001 aggatgggct acggatcatc aattgcaaca attcagttct tgattactct ggtattcatt
   177061 gttgtatata tgcggaatgt gttgaaaaag gaggtcggaa acgaatgaag aagacccttt
   177121 tctacatatt cgccaccctc ctggtgctag tctggctaat gccttttgtc ataacgatgt
   177181 ttacttcagt gaagagcatg gatgaactca tgatgggaag aagatggtgg gaaccaccaa
   177241 aagaactgag attcgagaat tacgcaactg cttggcagga cgccaatatg ggcagatact
   177301 ttatgaatac atttataatt acggtgcctt ccgttcttgg cgcgctgttt ctttccagtc
   177361 tgggcgcatt tgcattggca tggtacgatt tcagactttc caaaactatt ttgatgattt
   177421 ttgtgggcgg catgcttatt ccgtttcaga tgctgttgat accggtatat cgtatgtcga
   177481 tctctttcgg aatctacgat agctatatcg gtgttattct ctttcatatt gcatttcagc
   177541 ttggtttctg cactttcttt ttgaggaact tcatgaagac aattcctgca agtatattcg
   177601 acgctgcgat gattgacgga gccggtcact tcttgatata cagaagaata gttttacctc
   177661 tcgtagtacc tgctatggca gctttgggaa ttctcgagtt cacatggata tggaacgact
   177721 atctttggtc tctaatactg atacaaagcg acagattcaa gcctgttacc ttaggtcttg
   177781 taaatcttca gggtcagtgg attacgagct ggaatgttat ggctgccggg tctataattg
   177841 cagcagtcgt ccctctggta gtatttcttc ttttccagag gtacttcata gaaggactta
   177901 cagttgggag tgttaaggga tgaattggtt tggagcggct tattatccgg agcactggcc
   177961 tcgtgagcgt tggaaaaaag acgtaagaac aatgggagaa atcgggatca acgtagtacg
   178021 gataggagaa ttctcctgga gcgtagtcga gagaaaacga ggcgaaattg atttttccgt
   178081 tctcgatgaa gcggttgatc ttctcgattc agaaggaata aaagtgatta tgggaactcc
   178141 tacctgtacg cctccaccct ggctaacgaa agagttccct gagatacttc agaaggatgt
   178201 caacggactc gtcatagcaa gtggcagtag aaggcattac tgtttcaact cgaagatcta
   178261 tcgtgaggag acttcaagaa tagtcgaggc ttttgccgat cactttggca acgatccgag
   178321 gatagttgca tggcaaatcg ataatgaatt tggatgtcac aattctacag tctgcttttg
   178381 cgaggattgt gcggttgcat tcagagtttg gctgaagaaa aagtatggtt caatcgataa
   178441 gcttaacaaa gcttggggaa cggttttttg gagtcagatt ttcaatgact gggaagagat
   178501 agaaccgccg agaaggactc ttacttcgcc aaacccttcc ctggtactag attataaaag
   178561 attttcctct gattctgcga tagactatca taatctgcag agggagatca tcatgagaaa
   178621 gagcgaagca cctataaccc acaatcttat ggtaaacttt acggaaatag actataaaaa
   178681 gctttcagac tctatcgact tcgtatcatg ggacaactac attgtagggg attacgatcc
   178741 agatcttcaa gctctcaacc atgatctgat gcgctccctc aagaaacagc cctttctcgt
   178801 catggaacaa cagccaggaa gggttaactg gcgcactgtg aatccttcat acgaaagcag
   178861 tcagctgtca ttttgggtaa agcagtcttt tgcacacggt gcattcggat cgctgatctt
   178921 cagattcagg caactgcctt ttggttcaga gcaatttcat actggattaa tcgactatga
   178981 tggtgaaccc actgaacgtg cacaagcttt ttcaaaagct atagaaaaat taacagactg
   179041 gagtgttgtt gttccacaaa gagaagcggc gatttacatt gactatgaga atttttggat
   179101 aaatgatacg gacaatttga acaacaaatt cgatttgttg atagacggaa ttttgccggt
   179161 ctataagtca gttagaagtc ttggttataa cgtggatttc gtttttcccg gcgattcatt
   179221 ggaagggtat ttactggctt tcgttccttc aagtttcaaa cttgaacgat gcttcgtcga
   179281 agagctgagg aggtttaaag gggatatttt cttcacggct cttacggatc aaaaagatgg
   179341 gcgcaatttc atcaggaaga ccgaagaaag ttttatcact gaactaatgg gtcttagagt
   179401 cgttgatgca ggaggaattg agacgaaagt agagatatct tttgccgacc atgttttcaa
   179461 tggctccttt gtcgtagaga aaattctccc gagcgaagat tgtgaggtac ttgccgtcta
   179521 tctaaatggg ccttttacta atcatcctgc tctaataaag agagcgaaca tgtattactt
   179581 gagcacagtt cctgaaatcg tgtctttcca aactcttctt attgaagccg gaatagaaag
   179641 aaaagcaagt gggcctggaa gagttattaa gggcgaatct aacacgattc ttcaaaatcc
   179701 ttttccacat agcataacaa tggaagtcgg cgggaaggac tacactctta acgagtacga
   179761 ggtcaggaga gtccaaactg gcttatctaa gaataatcga tgagttcctt catgttttgg
   179821 aaagtaaatt cgagcggccc aaatctgaca tggacactac aggaagaact gatacttctg
   179881 aacccagctt attattgaag aactctttct cgcgaattct cggtctgcgt atacccaaat
   179941 cagcacagca tttgcctttc atccgacaat tgcgcgaaaa gcaaaccagc atttaagatc
   180001 gaaacacagg atgattcaga tagaatccct tctcatgggt cctattcatc aatgacaggg
   180061 cagccaattt tgaccggcgt ttctagcaat tctatttcag aagctgcgaa gtttccatca
   180121 catctctacc ccggaatcca ttcctcagga ttctagatct gtaacatcga atattgcgcc
   180181 gttgtgagag gccagccgcc tgggttcttc aatgccgttg aatcttgaga gttgctcccg
   180241 agtgaagaca agcaggccta ccaaatattt tcccgagttc ggcggttcca tgtggagagt
   180301 ctgcgagaat atctctagtc agttctcgtc cggcaccatg gggttgaccc tgatgataac
   180361 cgtttctcca aagagcaatc tcgatcacgt ggtaaacctc cccttctact gcaacatatg
   180421 ctttttcgcc atcatgactt ttctatcttc aagctcgtca actattgttc cactattgct
   180481 atcggttcaa cgcccgaaat tttgcgaaca ccttgcggag agtctttgca ttactcggat
   180541 gtcagttcgc tccaagcgag atgcatcccg gcctggtgag aatcgttctg ccgcctgttt
   180601 agtcgggaaa ggtcatagcc cttgcctcat atgttccgag ttcatcgaga gctactacgg
   180661 gtggtccggc cgattctagg ccagccgtct tattttcctt gtgtggggaa gtactttcag
   180721 ctcgcaagta aacgcccgca ccgaagaata aagagccatg tggttgccgg tagaccaaag
   180781 cctcaacggc tgtaaatcga aaaatgaacc acgaattgca acatgagcgg gattgtttga
   180841 cttgacgttg aatctcgata gggctgtaga aatgaatatt gggctgatct cagaaaatgt
   180901 agaaaggtca agatctttgc gaaaaatatc cgtggatctc ccaaaaaacg atattccgga
   180961 aatcacagca ccagcagtat caacgcaaga acaaatctat ccttctcata taaacctcct
   181021 tctcaccttg acggatccgt agatcgcttc cgtaaacgaa attctcgcct gaacctctcg
   181081 gaagttttca tgatttttgg aagttctgca gattgaagga aatggtgttt gcaaaggaaa
   181141 gcgcatacaa cctggatcaa tcgatactgc cttacctcaa aacttacaca aaaaaacaga
   181201 gggccctcgt gggccctctg ggttgtttat cagtagtaga taagttctag aagtagataa
   181261 aacgcgttta tagtttcgta ttgctcgtct atctcccagt agtaaaggtc gaaggtaccg
   181321 tcttcataaa cttcaaattg agcatatact tcggcgggaa tccctcctag atcaaatgtt
   181381 ccgtagaatt caactatatt gagatcgtca gttgaaacga aaaagtccca gtgtggttcg
   181441 tagaagaagc catcgaatgc ctcgcccagg gtcatccagt tgtagtaaac atcgaaatag
   181501 gagtttttga cttcggtgat cttggagtcc agtatcgaag caagcagcgt gtcgatagct
   181561 tcatcgtcct gggagacatc gtctattccc agatacgcaa tttcgaaagt gcgttcatcc
   181621 aagttcacag tgaattggaa aaccgtttct atagtttcat cttcctcgta aagaaagcca
   181681 atgtactcaa cgacatcctg attgtcggcg gtaacgaagt aatcccagct cccatctgag
   181741 aaataggtgc tgaaagactc gccgattatc ttgtcgggat aatcgtaaaa atagccttcc
   181801 ttaactactg agattgcgac gtctgcccgc tcaggcagga agatcttttc caggaacgca
   181861 aactgattac tgttgtcctg aggaacaccg ttgatctccc agtaattcat tatgaaggtt
   181921 gaatcttcct ccttgactaa gaagcgcatc ttcactgttg ccgcttcacc attttcatca
   181981 agataattgc cgctgaactc gacaatgttt tcggttccct ctttagaata tttccacctg
   182041 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   182101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgccgctg aactcgacaa
   182161 tgttttcggt tccctcttta gaatatttcc acctgggcat ttcaaagaac gcttcaaatg
   182221 attcaccgat caaattgtct ggaaagttat tgaaagttcc ctctgtgaca atgcttatga
   182281 accttgcact gtcctgaggc aatagccgtt gcgcaagagc aatacttgaa accgacaata
   182341 ccaataacaa cagaatagtc aactttttca tgaaaacaac ctcctttaat ctctcccttt
   182401 tttcactttg aatctgtttc gatccaatct tgtgtcagta agaaatgccg aacctctgga
   182461 tcctgttgcc cgattgctca agcacaaaca ctgagccatc atcactgaaa tagaggtcaa
   182521 tcggtctcgt aaacaaaccg ctcgccgttc ctgaacctcc ccactcgaaa aggaagctgc
   182581 ccttcgggtc aagaacaaca accatattat tcattttatt tatgtagtga agattaccag
   182641 agctatcgat tgttgaccct ccgggttgcc cccattttcc gctataaccg gagccccatt
   182701 cagcaacaaa gtttccctcg ggatcatact tctgaattct tctcactata tcgtccgaga
   182761 tgtatatgta tccattgttg tcaacactga gaccagatac tgttgcaaga ttcccgggat
   182821 tcattgcatc aaacatcccg acttttgctc ccacagtaag tataagcgtt ccttcattgt
   182881 cgaatacctg aactctttga ttgaaggcat caccaacata tatgcggtca gatttgtcga
   182941 ctgcaagata catcggtcca tcaaactgac ctggttccgg tcctttgctt ccccacgatt
   183001 tgacaaactt gttcttgctt gaaaacttct gtactcttcc attctgagaa tctgcaacgt
   183061 agatgaagcc ctttgaatcc accactatat cgtggggaaa gagaaactct ccgttttcgc
   183121 tgccatagct accaaatgag gttttgtgtt taccgttttt gtcgaatacc tgaattctgt
   183181 ggttcaaagt gtcagcaacg tagactaaat cctttccgtc aacgaaaatc ccttgcggat
   183241 cgctgaattg accattctta gttccctgaa ctccccaaga cttttcgaaa actacagaag
   183301 aaccaaacgt tggaacggaa acaagaagtg ccaaaagcaa aaggacaaaa ttcttcaaag
   183361 acttcacccc ttcaagcttt ttatttgctt gtctgatatt aacactcgaa gtgccagaaa
   183421 tttctcgttc ttatacagtc gttttaggaa atgtccgtgg agtgtggcag attgcatcac
   183481 aagattgata gaggagcaaa accaagcaaa aaagtgatgg aatgctttta agagcaatta
   183541 aagacttaat ttgagaatac agggcgttgt cagggaggca gcagtgctct aaactataga
   183601 tgagccacag ttcccctcat aagcaatctt ttgtggtcaa ttttatgttg tagcgttgag
   183661 tataaaaacc cattacaaac cagattggat tggaggtggc ccaattggca aagatgatca
   183721 agaagaacta tttacttgct ccaggcccga cgccggttcc tatcgacgtt ctgcttgagg
   183781 gtgcaaagga tacgattcat cacagaactc cgcagttcaa gaagattttt gaaaacgcgg
   183841 taaacgggac gaaggaggtt ttcaggacac aaaacgatct ctttatcctt gcttcttccg
   183901 gtacgggagc gatggaaatg gccgttgcaa acatagtcaa tcccggagag aagttaatag
   183961 tctgtagcgt tggaaagttt ggcgagagat gggttgaact tgcgaagact ttcggtgcgg
   184021 agatagtctt ggttgaaaga gattatggag acttctacac cccggaaatg gttgaaagag
   184081 ccctggatga aaaccccgat gcagttgctg ttcttacaac ccttagcgaa acatctactg
   184141 gaacagtaat ggatatcgaa ggcataagta aagttgtgaa gaaagcgggc aaactcatcg
   184201 cagtagacgg cattagtggt ttagttgccg aacgtttgct taccgatgac tgggggcttg
   184261 atatagttgt atctggctct cagaagggtt ttatgctacc tcctggactg gctttcatat
   184321 cgttcagtaa agaggctatc gaaaagtcca agaacgttag ttctaccagt ttttacttca
   184381 acctgaagaa atacttgaag gatcctattc catggacgcc agctgtcaac ctgatctatc
   184441 agcagagtct tgccgtaaaa atgcttctcg aagaaggaat ggaaaacgtg tgggcgagac
   184501 acgaactaat ggggaaggca acgagagaag ccataaaagc catggggttg gagcttttct
   184561 ccaagaggcc gggaaatgtg cttacttctg tcaaggtgcc tgaaggtgtc gacggaggca
   184621 aaatagtctc cataatgaga gacgaatatg gagtaacgat agccggtgga cagggttcga
   184681 tgaaaggaaa tatcttcagg atagcccacc tcgggtatat gtcggactac gatgtcatta
   184741 ttgccctgac atctctcgag aaagtcctca gaaggctagg gttcaaggtg gagtacggaa
   184801 ccggagcaag agttgctatg gagattttcg agaaggaggg cgcttgatgc ttagactaca
   184861 cgctaacgat cctctcgaca aggatgcaat gaagatcctt gaggacagca agctgttcga
   184921 gataactgct gagcatctag ataaagatga gatgcttaag aagatgcccg aaatagagat
   184981 cttggtcgtg agaagtgcaa cgaaggtaac ctctgaggtg atcgatgccg gaaagaagct
   185041 aaagttgatt gcaagggccg gagttggact ggacaatgtt gacgttgagg ctgcgaaaag
   185101 gcacaacata atggttagaa acacacccgg agccaacgcc atctcggtgg ccgaactcac
   185161 tttcggtttg cttctcagcc ttgtaaggca cattccaaga ggtacgtacg gcatcaagga
   185221 aggaaagtgg gaaaagaagg aactgaaagg tacagagata ttcggcaaga cgattggtct
   185281 tataggattt ggggcaatag gccgagaagt cgcgaagagg gctattgctt ttggaatgaa
   185341 tgtctgtgct tacgatccat ttgtgaagga aaccgagatg gaaattgaac ttgtttcttc
   185401 actggattct ctattcgata aatcagatgt catctcgctc cacattcctt taacagccga
   185461 aacgaaacac attgttggtg aaaaagagat ctcagcgatg aaagacggcg ttatcatcat
   185521 taacacaagc cgagggggaa cgatagatga gcaggcccta tataacgggt tagtttctgg
   185581 aaaggtttcc ggagctgccc tggatgtttt tgaagtagag cccccctctg acgaacttag
   185641 aaggaaattg atcgggttgg gtaatgtgat atgtgttcct catgttggag caagcacgac
   185701 tgaaggtcag aaaagggttg gccttgaaat ggccaagatt atcgtagaag aatgcaagag
   185761 catgatataa ttcacatgat ttttctggtg gaggtgtaaa cgttgaaggt ttacgtggac
   185821 aaagatactt gtattggatg tggagtttgc gagggaatct gtcccgaggt tttcaaaatg
   185881 aatgatgatg gaaaggccga ggttattgtt cccgagacgg aagcggcttg tgctcaggat
   185941 gctgcagact cctgtccagt tcaggcaata aaagtagaat agtttccaat tcttaacgtt
   186001 cacatgtagg ggcagacaaa tgatctgccc ctttctatag tggaacacaa aatttgtcta
   186061 ttttttctga gtgttaagtc tgatatagta gtaattgctt cggaaagatt tattgtaaat
   186121 atttcctatt ttgcggttcg cttcagggtt atagcattat tccgtggagg tcgttgtgtc
   186181 taattctatt ataagcactt tgaagaagaa ggtctcaaag aaaacgtggg acaactggtt
   186241 ctccacattt gagttgaagg cagtcgaaga tgaaagggtc gttttctcgg tcgccaacct
   186301 gttcatcaag gactggcttc agacaaaata tggagctgtc atttccgatt ctatagccga
   186361 gattctcggc aggcgagtct cttttgaaat tgtgtacaag aacaaggaga ctccctctgt
   186421 agattcgacc agcgaccaga tatccggttc tctgttgaag agaaagcctc tgatgatttc
   186481 aaatctaaat ccagagtata cgttcagtaa cttcgttgtg ggaagtgaaa acaaagccct
   186541 ctatgaagtg gcgctggacg taacccagaa tccggggaag tataatccct tctttgtcta
   186601 tgggggagtg ggattgggaa agactcatct tctgcaggca atagctcagg agaccatgag
   186661 caactttcct gacaagaagg ttctctacat aacaagcgaa cagttcatga acgacatgat
   186721 tcagtcgata aaagagaaca acattcagaa gttcagagat cattacagga agaaatccga
   186781 tatccttctg atagacgata ttcagtttct aataggcaag aaaggtgtcc agaatgagtt
   186841 tttccattcc ttcaatgaac ttcacgattc ggggaaacag ttgataatct gctccgacag
   186901 aaatcccgag gagttagaga cattccacag caggcttaag tcaaggttcc agatgggaat
   186961 gctgatgtca attcaagaac cccgcccatc taccaggttt catattgcca agcagcttgc
   187021 tcaaagggaa tccgtatctc tcccggatga tgttgccaaa gttctcgcgg acaacatcga
   187081 tggtaacctc agaagattgc gtggagcaat tataaaactg attgtacaca gtagtgtctt
   187141 tagatcacag atagatctat ctctggcaac ccagattctc cagtctttca ctggctcgat
   187201 aaacgttcca gcattccaga gaccgattga ccagatctac tccgctattg agaagacgat
   187261 gaaggttact aagaaagaaa tcgaatcggg gagcaggagc aaggacattg ttcttgcgag
   187321 gcagttaact atgtacattc tgaagaatca ttttggtaag caagtaactg aaatagcgcg
   187381 tgaaaccggg aaacagcact caacggtgat acactcactg aagaagatcg acaagagtgt
   187441 aatgatgggc aagggagcaa caaaactcct gtacgacgat gtcatcggga taataacttc
   187501 aaattcggcc gcagtatgaa atttcgttat gctgccagat ctctttcaac ttttcttgtc
   187561 aagccaacta atccggacaa ccttaatctg aatatttctt tgtggaaatt ccaccactct
   187621 ttatccaaca gatagtagat tcagtgaaat ttacttagtc tggaaggtaa gaatgaaata
   187681 tgcagttagt ttcttgctat tcttggtatc tgcagctacg atcatcggga caatttccgt
   187741 ctctcaggca agcagacttc gagaatcgat gaatgagaag tcaggagcat ttccaaacat
   187801 ggttgatgga gtctttgccg taagtgaagg atgtataagt ctggaggtga tagattccag
   187861 tagagaataa tcgaaatacg aatactcaag aatcaatcgg atagatatga cataagagct
   187921 taaggaactg cggatagagt acttgaagca cagagtcccg atgttgattt ggttactgga
   187981 gctacaaccc caagcgagtc tatcgttgcg gcggtcaaaa acgcactaga tgaggcttct
   188041 tcatttccca aataatggtg catctatcgt tacagttctc gatgtatccc actaacagtc
   188101 ccagaacaga atgagattca gcggtggaac cgtaattgcc gagagccttc ctttaagttt
   188161 gaatcttaca tctctgcagg ttcgaaaagg ctcagtatcg gattgtttct ttccccacaa
   188221 atctgccaat gattatctat tcccgataac agaatacttc cctctccgat gtcgtaatac
   188281 ctctttcctt cgtaagaata ctcgagtgta aaagagaccg tcatggttgc ttccagagaa
   188341 tctctaaggg tttcttcaga gtgcagttct atacttatgt cttcgtagag ctcgaaaaag
   188401 tccgagtaga aagaggcaag gctgtgcttc ggaagaattc tgccggaggg attacccgga
   188461 gtagccaccc acacctcttc tgagtagagt tcaataattc tgtcttcctt ggctctgttt
   188521 atttcgcgct ccagtttggt taggacactc tccggtctgg ccagcacata gttaatgcat
   188581 ccggaagtaa ggatagataa tgaaatcagc gaaagcaaaa gaaagatgaa atttcttctc
   188641 gacaaagctt cacccccagg ttaatcttct cctcatatga tagcagatcg acaggcctaa
   188701 ctatcctatg ttcttggaga atcgtatgaa ttagagatct gtaagatgaa cttgtggaga
   188761 gtcgaacaaa atcatcactc ggaagtctta caggtggtaa gctagtctag cggttctgtg
   188821 aggagattag tctatgagaa tgaacattgc ttatcttttc ctggtaatct tgactactgt
   188881 tgttctatct gcaacctttc tgaacttcaa agacgactac tcgaacataa gagtgcagac
   188941 gtcagatgta agaaggggtg agtctttttc catttcagtc cccaaatcca cctatgaaag
   189001 atctactctt tcatatgtaa ggggtgcaac actagaaagt ttctacagcg attctgatct
   189061 cgtgacatat tctttctgcg ctgatgacaa gaatgcggtc attgttgttg atttcaaggg
   189121 cctgttcaga gccagcaaaa ccctttcctt ggagttcaat gatttcgttg acagtaattc
   189181 cgacggtttc cctgataaac tggtgcttga ctgtgaagac gccgagaact tcagagcatg
   189241 gtttgtgaac attgctgcct accaggttat tgagttctca aataactgga aatccgaaga
   189301 aagagattgt agcgggctga tcagattcgc cgccagagaa gctcttaaga cgcacgacga
   189361 agagtggttt tccgagacag ccgtagacta tgatctgtgg cgtgagaaga ctggaattga
   189421 tctgcgaaag attccggacg ttagagagta taactaccca gatatcccga tactgagaaa
   189481 cagaatcttc ataagcaacg acggagcctt cacttacttt gccgatgctt acaacctggt
   189541 tcgtagtagt atggtattta agggtaggga tctgggagca gcaagacctg gcgacataat
   189601 tttcttccat catccttcgc cgtcaacctt tcattcaatg atcttcaccg gtgatggcct
   189661 catataccat actggacccc tttccgaaac cgattccggt gtcttgaaac tctggagtat
   189721 ggatgattac gtaagaacta tgccttacca atggcaacca atatatacta acgaaaacta
   189781 tctaggggta tacgctttca agtttctgcc ccagaagtga tggagggtga acgcgatgag
   189841 aaaggtatgt gtgcttcttc taattcttct tttagcaggg ttaacagcga ttgcgtttga
   189901 gattcagcaa tatggatacg agctgagctc ctctttcgtg gagttctatt cgcttgatta
   189961 tcttccaagt gtctacaccg atgacaagga ttctttcatc gacatcaaga tctacgatct
   190021 agacgaggaa gaccttactg gagcgataac aattggtgat gtaagagttt ccggagatcc
   190081 tctatatgct tttaggcaga cagttaagga gaaacagaga atctacttac caagagaaga
   190141 actcatgggt ctaagattaa tagttgtttc gtcgcagtcc ggtaccgcaa agctaatgac
   190201 gagttttcgc atggttggag cccagttgct cgctcatgaa gacggagcga tgttgaggct
   190261 ctggaagaaa gatgatggga gcttcctgga tcacttctcg ctttacagac tcagtgatgg
   190321 ggagctgctg gggcaatctg aagagggagt tttcgtcttc gattaccttc ctaaaaccga
   190381 agaggcaata cttgttcagt cttccgaggg atcgattcta tggaggccag gggagtatga
   190441 ttactatttc tcttccaggc acatctcttt gacgtttaca gacagaccgg cttacaaagc
   190501 tggagaggta gttaacttca gatccttcat cagagaagtg actccatcgg gttataagat
   190561 accggaaata gattttgtcg aaatcgagat agtcgatcct ttgaacagag gagtttacgc
   190621 tcaaactctg gagccagacg atctgggatc agttgtcggt agtttccgaa catactccga
   190681 aataaccaga ggatcttaca gaataatcgt tcgatggggt gaaaacgagg attactacta
   190741 cttccagatt gcagattata agaagcctac tttctttaca ctggcagaac ccacaaccag
   190801 tgccttcaga aaaggagagc ctgtcacaat tgatgtctcg tcggaatact actttggaga
   190861 tccggtctct cttggaaagg tagactacac gatctacaaa ggcactcaat acattgacaa
   190921 tggctctgca agactggacg gttcgggaaa gaccgtaata ggatacacag gggagcttga
   190981 aagcggagcc tactacgcga tagtgactgt gtcagatgac acaggtatgc agtcacggag
   191041 tgtggtcgat ttcaaaattg tacagggaag tttcgatttc ctagtggatt atagattcac
   191101 cgaaaatgaa gctcttgtaa gaatcgaaac caggttgaat gacgatactc ctgtctctaa
   191161 ggaattcgag ataaaggcct ggtacgagga atcagtaatt ctaatggata aaggcgaaca
   191221 cgttgaaaag aatctaaaaa taaacgtctt caagggtgaa ctctcaaccg atagcggcgg
   191281 aaaggcagag gtatcgattg atcttaggca agttcctcca gatacggtag tgtactttga
   191341 gataaatggt tcgccttcag gggaacctga aatcgtaaat acctactcag tttacaccgg
   191401 aggttactat gactattatg ggtcgatatt aatcgattcg gtgaaatctg cggagccagg
   191461 gtctagtgca gaagtggtct tctacacacc gtcaccgatg gatctgtgga tcatttctga
   191521 tttttccggc gaattcagtc aattcccatt cagttcgact gaagggaaaa acacggtcta
   191581 tatagaagta ccggaagggt atgcctacga taatttcatg ttattcatcg tcggatacag
   191641 ggattatcag attatccaat caagcctgat cgaagtccaa acgtcttcaa gagagctatc
   191701 aatagagact ctcactcagg aaaggtacag tcctggtgat acagtgaata tgaaaatcca
   191761 ggttaccgat tccgaaggca aaccagccga agttgggctt accgtttccg ttgtatccca
   191821 ggcaatgcta tccatatttg aaggggacta cgatcagtgg aagggatcgt taggcagtcc
   191881 tttcaacaga gggtttaaca caatcagcat caatgatctc tactattccg cctacccatc
   191941 tatcgcaaaa ctcggagatt tgcttgaatc acaaccgccg gccgcagagt ccaaagttac
   192001 gggaggtgct ccgctcggca tgggcgatct aggtggagat gagaagagcc ttacctctga
   192061 tgtaagggca agaaagctat tcagtgatag cgccttctgg tctaccggaa ctttcacaga
   192121 tgagaacggt caggctgaac tgtcttttgt agtgcctgaa gatctagata cctggacagt
   192181 aaggacttta tcttccagtg cgaacggtga ttttagctac agtaaatcga gtttcgagac
   192241 atggaaacca atgaccgtca gcagttttct tccagagttt cttattgctg gagacaccgt
   192301 taaccttgtc ttctctgtta agaataacct tgattcaacc ctacctgtaa tcaccggctt
   192361 ctatctcggc gaagaagtaa tagaggaaaa gggttcttca ataaaagcat ttgagagccg
   192421 ttcattcagc tacgaggtca atgtacagga tctcttgtcc tctgagaaag atcagaagct
   192481 caaagtgaag tttgtcgtca agggaagcca cggctcagac ggagtcgaat acgagattcc
   192541 cataaagccc aggttttctt actctcgatt cggttccctt gatttccttg aaggaaacaa
   192601 gactctcgaa ttctcagaaa actctgcagg aacgattacg atttcttcca caattgatcc
   192661 tattcttctg gaggcggtca ggtatttggt agattaccct tacgggtgtg tcgagcaaac
   192721 gatgagcaga ctgcttcccg cactagcagc ttctcagcta ttggaagaag cggatactgc
   192781 tttcatcaac aaggtctcaa gagtggtaag tgaaggactt gagaggttat atgggtacca
   192841 gcattacgat ggaggctggg gctggtggaa ggacgataac tccacgccat tcatgaccgc
   192901 gtatgtgatg ttgggtttgt atctcgctac agagaacggt tatgttatca ataaagatgt
   192961 ggtatctatg ggctattctg agatgaaaac tctctacaaa gaagatccag atcccttcct
   193021 tcaatacgtg atagttctct tcagcagaaa actgagggat cccgtaggtt ctgtcgtcga
   193081 tttcaaaggt gacgttcctt cgataattct gacttcgctg gcctacgaag cgttgaacat
   193141 ggacgacaag gctgaagatc taattgaaga agcgatggaa tacgtagatc tggatgcaga
   193201 cgacgtgatt tccgggacca gttttgatta tttcttcgac gatatagtta ctttgtctct
   193261 gcttctgaag gcttcggtag atctggagat gccttcggag gacatctttg agatttcgaa
   193321 gagacttctg aggatgggta acggaagcta ctggtataga acatcgtcaa cggcgctggc
   193381 aattctgtct ctgtcttccg tgagtagctc gctttcggaa aacgcccaag tggaagtttc
   193441 attgatcggc ggagaagttc tttacagcgg tgggctttct gaatcaatta caatcccatt
   193501 ttgcgaagag aacgtaattg tcgaaagctc agaaatggta gctgtctcag taaatggtga
   193561 gaaagcagtg gcagctgagg agatagaacc tgaaaactcg ggtttgatcg tcaaaagagt
   193621 tctgagaagg aagctcgccg ttcccatgga tgacactcat gtccttacaa caccgcagat
   193681 tgattcacca tacgttgttt cgtccattaa gaccctatca ccggatgaag ttggttctct
   193741 cgagataaat gtatcgaaga cggttgaaaa catgcagatt tcaatcaaag acggagagct
   193801 gcttcttgga gaatacggat tgggtcttaa ggtgtatgaa ggcgatgttc tgggaatcag
   193861 caacggcgag ataattatca gagcctttga agagagctac tatgatgaac cgacagtcga
   193921 gatctattca gttagactcg gtaccccagg ttctcttacc gctggtgaaa caatagtctc
   193981 tgaggttcac attaagattc cggaagacgt tccttacgtg gttgtcgaag atatgctgcc
   194041 ggcaaccgga atatccgttg aggagaatct ggaagcagac gtgctcgggt acacgaagtt
   194101 ctattacttc gactactact ggtggggcta cacattcaaa gacgcaagat acgaccgaat
   194161 tgccttcttc tttagggaag gaggagagtt tgttacgaaa aacagctgga ggatactcac
   194221 cagtggtgaa ttcataattc caccggttca ggcatgggca atgtacgatt cgaattaccg
   194281 ggcaaatacc gaatctatag tgctggttat cgaatgaaat ctctggcatt ggtggcattt
   194341 ctattttttg gaatgacagt tttttgcttc acaagcacat tcgtttcatg ggaaatgccg
   194401 gaacttgaag aatataaagg tcttcttgaa aggctcgtag gcaaacccgt cacgggaact
   194461 taccgcttaa taatcgcttc atcgaccgga gagttcatgg aactctccgg cctaggctat
   194521 tggttcattg cagctgcaga agatagcacg atctttgttc agcctctgag tttggtcccg
   194581 aatttgacgc aaacggtggc tcacgaaatg tgccatctct ttctcaggca gtatcgattg
   194641 ccctactggt tagaagaagg aatagtgtgt tacgtaacgg gggaatggat tggtcgaaaa
   194701 gagctgatac tcgaccgggt ggaaagcctg gattattcag agatggattt catgacatac
   194761 agatcttaca gcttcagctg ctgggtagaa gtctcgaaaa ttctaagaga aagaacgttc
   194821 ggagaactgg ttgttgcact tggagaaggg gttgatgagc aagttgaatg aacggaagat
   194881 aatagaatgg gatctgacaa ggctttacgc ttcgcttgat gacgagacta tttttgaaga
   194941 catcaagacc gtcctaagag aaaaggatga actcgtcaga gattacaggg gaaggatctc
   195001 aggtgaaaat attagtgctc gtgaactgag ggggttgctt gaaaaatcgg agtctcttct
   195061 gaatcgtgcc ggcaagctgt cgatgttcgc ttatctaaat cattctgttt taacagcaga
   195121 ttcagcgact caaaaactag ttcgtaaaat ggacgatcta gaggcggcgc ttgaaagtgg
   195181 actgatgttt attaagcttg aactggcgcg agtttcagac gacttctttt cagagttgaa
   195241 tgaatgtgat gagttgaaga actacagaca ttacttcgat cttgttcgag cgttgagaga
   195301 ccatcagtta tcggaacccg aggaagacat aatagtaagg aaagacatta cggggaaaat
   195361 ggctatgctg aatctctttg acgagtttac ctcgactttc gaatacagaa tctttattga
   195421 tggagaagag aaggtcctta ccgaaagcga agtcgagaat ctaagaagcc atggagatga
   195481 agggataagg aaaaaggcat ttgaaagcct cttcagaaaa acagaggaaa atagtgttat
   195541 tttgacgaac atctacaact ccctcgccaa ggactgggat cttgacgcaa gcaaaaggca
   195601 cttctcctct ccaatttcaa tgaggaacat tgaaaacgag gtttcagatg aatccgttca
   195661 gaatcttata gacgcgacta cgcaaggtta ttctatcgtg caggattact accgtttgaa
   195721 ggcccgtgtc atgaacaagg aaatccttct tcacagcgac atctacgcac cgctcgcaga
   195781 ttcgagagat atatttagct ggcaggaagc gaaaaatatg atacttgaag taatggggaa
   195841 ttttgattcc caaagtggac gaattatttc cggttttttc gatgatgaat atattcatgg
   195901 atcgataatt cctggaaaac gtagtggcgc attttgttat tacgcctcac ctgaagtcca
   195961 cccctatgtc cttgtgaatt ttggcggaaa aactagcgat gttttgacgt tggcacatga
   196021 gctgggtcac ggcttgcatg gcgtgctttc ttcaaaacag actcatctta actacgagac
   196081 gcctctcacc atggctgaaa ccgcttcgat tttttcggag atgcttatga ctgattttct
   196141 tctggacaat attagtggaa aggcggaaaa actttcgctt atctcaaaaa gaatggaaga
   196201 gatactggcc tcgacggcga gacaaaatat gttcacacga tttgagatgg ctgcccacag
   196261 tattatctct agggagtttc tttcgttcaa agagctgtct gatctgtact ttgaagaact
   196321 aaagctgatg tttgcagaca ctatagtatt ttttcctgaa agtaaattcg aatgggctcg
   196381 cataccacat atctttcaca cgccttttta ttgttatgcc tacaacttcg ctcagttgct
   196441 ggttatctca ctttacagga tgtaccttga ggacggggat gtgttcaagc gaaggtatat
   196501 acgactgctg gagagcggta gttcagactc accggagatt ctgctgaaat cagttggaat
   196561 agatatttcc gagccttact tttgggaaaa tggacttcac ttcatccagg agaattttct
   196621 ggaaagattg cgggacataa tcgattcctg atagtatatg taggttataa tttctgtgtt
   196681 ctgatggata gaattttcct ttttgcgtct cttttcaagg gtaacatagg gggcttaaat
   196741 ggcaagagat cttactcagg gcaacattct gaaaaatctt ctcgtgatgt ctgttcctac
   196801 aatgattgga ttcagtgcac aaatggtcta cgacatagtg gatattttct ggataggtcg
   196861 aatctccggt gaagccattg cgggagtcac catcttcacc actctcttct ggattgtaga
   196921 cattcttaat tcaattatcg gtcagagctc tgtttcgttg atttcacaga gctttggaaa
   196981 gaaggattta gaagggacga acagggcgat agaacagacc attactttca agttcatagt
   197041 tgcgttgatt tccgcactgt tagttgctgc atttctaaaa ccttctctag gttttttcag
   197101 caacgatccg acagtaataa cagcagctct agattacggt tatatccggt tgttcttcct
   197161 gccgatgatg ttctcatctt actccgttaa tacggcttta agatgcatag gcgacgcgaa
   197221 gagtccgatg tacataatga tgatggctag cgccctcaat gtggtactcg atccgattct
   197281 gatgtttagt cacattcccg gaacgtcgat tccgggtttt ggactagggg tcttcggcgc
   197341 agcggtagca accgtcatag cacagaccgc atcgtttcta cttggctttt ttattctttt
   197401 ctcgggcaaa gagggagtca aaccgaagct atcgaggctt ttccgcctcg ataggcacat
   197461 tgacaggaag cttctaacta ttgggcttcc aacgggactg gagggtttct tcagaaatct
   197521 tgccgccgtt gtggttctga aattcgtggc actatacggc acaactgccg ttgctgctgt
   197581 aggagtaacg ggaagattgt tcggacttgc attcatgcct cttgtgggac ttaacatggg
   197641 gggttctgcg atggtcgggc agaatttagg agcagatgat gttggcagag cgagagcgac
   197701 tgcaaaaacg gcgggattaa ttggattctt cattatgttc ttcttcgctc tgattgcgtt
   197761 ctttggtgga gagatagtta ttggaatctt caatgatgat ccagagatca tttcatatgg
   197821 cgcgagcttt ttgaaatatg gttctcttgg aatttcagtc ctggcctatg gcttcggtct
   197881 ctcctcagtt ttcgggggat cgggttacaa ctttccattc gtagcgggca gcgttgtctc
   197941 cagatggctg attcaggttc ctatgctgat agttttcgta gcgttcatga aagctagcat
   198001 agtctgggtc tggctttcat atgttttttc cgatatcgca gaggcaactg taatgatttt
   198061 ttactatctg aggggaaaat gggaaacaag gagagtctcc tagtcgcgaa tgtaataatc
   198121 attctgtcta gaaaagttag ttaaattttt cactttcttt ttcacagtat tctcatttcg
   198181 tgcccaaaca aatttcagac gatctgctat aattcagatg aacgggtgat taagatgtct
   198241 gaagttttgt ataggaaata taggccaaga aatttcaatg agttagtcgg tcaggatcag
   198301 gtaaaagaga tcctgggaaa ggcgatagag aagaacaagc tttcccacgc atatatattc
   198361 tcaggctcaa gaggtactgg aaaaacgact agcgctcgta ttcttgcgaa gatgcttaac
   198421 tgtctttcgg aaggatcgga aaaaccttgt ggagtttgcg attcttgcaa agcaatcgac
   198481 tcctcttctc atatggatgt tgtagagctc gatgccgcct cttatagagg catagatgag
   198541 atacgaaaga tccgagatgc ggtttcttac agaccggtaa tgggaaaatt caaagtctac
   198601 attatagatg aatttcacat gcttaccaga gaggctttca acgctcttct caagactctt
   198661 gaagaaccgc ccgataaggt ggttttcatc ctggcaacta ctaatctcga gaaagttcct
   198721 gaaacgattc tttcccgctg tcaaatcttt tacttcaaac ctttgaggga agaagacata
   198781 gtaacttatc ttgaaagaat tgcgagagcc gaagagcttc aatacgacga aaaggctttg
   198841 agatacattt ccaaggcggc acacggagga atgcgagacg ctgtaaatct tatggagagg
   198901 gtaatctcat actctggtaa cgttaaggaa gaggcagcta gaaccgctct tgggatcctg
   198961 cccgaagagg taatagagga atttctccag gcgttttcat cgggaaaccc atcttcagta
   199021 cttgagattt ccgaaaaaat ccagtctgga ggcttcacgc acgaagtatt tcttgaacag
   199081 gcgattgatg cggtcaagga acgacttgtt gagcgaccga gtgacgattg tttcaggctg
   199141 ctttcggctc tttgggaaac aaataaagaa ttaaagtatg ccgaagacaa acagacaaca
   199201 tttgaagtta tggtcctcct taagggcggc ttcggaaaac agaacggcga agacacgcaa
   199261 aaatccgaag tatctccgat cgctgttgac gaaaatagtg agaagcctga aaagcttgag
   199321 gtttcagtca ccgaagaaag tgaagagatc tcattattca tcgaacatct tagaatgaag
   199381 gattatatat tgtggtggac tttgataagt ctggcggaaa tcaggaaggg gaatcgaatc
   199441 gaggacttaa taatagaacc agatagcgaa ttctcaagtg agcttctgaa ggaaagaatg
   199501 gaatcgataa acaaaatcgc tgaagatatt atcggcaaac gattcgttct ctctgacaaa
   199561 gcggctggac aagaggccct tgaaagcctc gacagagatt caagagagta ttttgaatcg
   199621 gtaattacag gcctggggct taagagtgat atagagactg gaaaaataaa gatagagctt
   199681 gaggaggaat aactatggcg aagaaattca ggggcctcgg aggacgaaac tacggaggtt
   199741 caaggaaagg ttccaatatg ggcgaccttc ttaaacaggc gcagaaggca caagaagaga
   199801 tggaaaatct tgaagatagt ttcaagtcga ttgaagttac cgcttctgca ggtggcggtg
   199861 caataaacgt agttgcaacg tgcgactatc gcatcaaatc cattgaggta gagccggaaa
   199921 taagagaaga ggatttcgaa atcgttcagg atttgataat cgcggggata aacgaggcgt
   199981 tagctgaagt aacaaagagg agagaagagg aaacagcgaa aataacaggt gagttcaatc
   200041 taccggacaa catactttag gaggtgtaac gaatgcacaa agtagggatc aatggttttg
   200101 gaagaatcgg aagacttgtc tttagagaga tggtgaagcg cggggagttc gacgttgttg
   200161 cgatcaacga cttgacagat gccgctactc ttgctcatct tcttaaatac gactctgttc
   200221 atggaaggtt caagggctca gtagaagcta aggaaggagc catattagtt aacggaaaag
   200281 aagtgaaagt attcgccgaa aagaatcctg caaatcttcc ctggaaggat cttggcgtcg
   200341 agctcgtcat agaagcaaca ggggttttca gaagcagaga gaagaccatg cctcatattg
   200401 atgctggagc gaagaaggtc ttgattactg cccccgcaaa gggcgaagtg gatgccacaa
   200461 tcgtgctcgg tgtcaatgat gaggttctta aaccagagat gaagattgtt tccaatgctt
   200521 cctgtacaac taattcaata gcaccgataa taaagattct gaacgacaat tttggaattg
   200581 agaagggcta tcttacgact gtacacgcat ataccaatga ccagagaatt ctagatcttc
   200641 ctcacagtga tttaagaaga gccaggacgg ccgctgccaa cacaattcct acatcaaccg
   200701 gtgcagccaa ggctgtaggg gtggttatcc cagaattaaa aggtaaactc gatggaatag
   200761 cgatgagagt tcccgttacc gatggttcaa taaccgatct gacggtcgtt ctcaaaaagg
   200821 acactacagc tgaagaagta aatgaacttg tgaagaaagc tgcagaaaac gaactgaaag
   200881 gaatagttga attcaccgaa gaggagcttg tctcttctga catagttgga acgactgttt
   200941 ccgcgatatt cgatagtaaa ctgacggccg taatgggtaa ccttgtaaag gtttgttcgt
   201001 ggtatgacaa cgagtacggt tactcgtgca gagttgttga tcttgcgaag aaaatgatgg
   201061 aaatgtaact ctgagttttg tcgacggggc cccgaggccc cgttttattg aggagaggag
   201121 gcttaagaaa tgtctaagac attgactctt aaggatatcg atctggctgg gaaaagggtt
   201181 cttgtaagag ttgattttaa cgtacctcta gataaagaga cgggtgaggt gagtgacgat
   201241 accagaatcg tcgcggcatt gcctactatc aatgagatta tcgaaagagg gggcaaggcg
   201301 attctcgttt ctcatctggg tagacccaaa gggaagagag atccgaagta ctctctcgaa
   201361 aaagtcgccg agaggcttca gtcactgatc ggtaaacccg ttactttcgt cccggactgt
   201421 ataggagaag aggttgagaa ggccgtttca aaaatgaaca atggtgacat tcttcttctg
   201481 gagaatgtca gattctactc tgaagaagag aagaacgatc cggacttttc gaggagactc
   201541 tcatctattg cagatattca cgtcaacgat gctttcggca ctgctcatag aagccacgcc
   201601 tcaaacgtgg gagttgctcg ttatttgacc agtgtgcccg ggttcctcat gcaaaaagag
   201661 atagaaatgc ttgggatggc aattgagaat cccgaacacc cctacgtggt cattcttgga
   201721 ggagcgaaag tctcagacaa aattggagtg atcaccaatc ttctggagaa ggcagatagg
   201781 atcctaatcg ggggcgcaat gatgttcacg ttcctaaagg cccagggaaa gagtgttgga
   201841 gactctctcg tagaagaaga caaaatcgat cttgcaagag agattcttca gaaggcaagg
   201901 caaaagaatg ttgaatttgt ccttcccgtc gacacggtga tcgcaaagga gattgcagcc
   201961 ggtagtgaat cgaaagtagt cgatcttgaa gaaggcgtgc ctgctggttg gaaggggctt
   202021 gacataggtc catcaacaat agaactcttc aataagaaac tgagcgatgc taaaacagtc
   202081 gtttggaatg gacccatggg agtcttcgag atcgacgact tcgctaaggg aacggagtcg
   202141 attgcgagaa cgctggcctc gctcaatgat gcggttacga taattggcgg cggggatagt
   202201 gcggccgcta taaacaagtt tgggcttgct gaaaaggtct cccatgtttc gacggggggc
   202261 ggcgcatcgc tagagatgct cgaaggtaag gaaatgcccg ggataaaaag cctttcgatt
   202321 gagggtggta aaaaaaaacg tagattgatg gtcgcgggaa actggaagat gaacaagtcg
   202381 cctaatgaag ctcgaatgtt tgcaggcttc ctcgcatcgt caatcggtaa tgaaaatgcg
   202441 gtagatgtcg ttgtctttcc aacaagtctc tcggttgcag gggtggccga tattctaaag
   202501 gacacttcga tcaaattcgg agtacagaat atctatccag ccgacagcgg agccttcaca
   202561 ggagagatct ctgcatcgat gctgtcagat cttgctgttg aatatgttct ggttggtcac
   202621 tctgagcgca ggcacatttt cggcgaaaca tgcgagctga ctggtgagaa gatcaagtca
   202681 gtcttgaagc atggcctcac accgattttc tgtgttggtg agactcttga agaaagagaa
   202741 gccggcagaa tgaaggaggt actggaaacc cagattagaa agggcttttc cggtcttgga
   202801 aagagcgatc tagagcgcat aataatcgct tatgagccag tttgggcaat aggaactggc
   202861 gtggttgctt cgccagagca ggccgatgaa gcgatgaagt ttatcagaga tctaattgcg
   202921 tctctttatg gcgttgattt gtcagagagt gttcggattc tctatggtgg aagtatcaag
   202981 ccagaaaact tcgaatcact catttcaatg gagaacattg acggtggttt agttgggggc
   203041 gcaagccttc aagaaagctt tgtacagctg gttgcaatag ctgaaagtca cgcttgacaa
   203101 ctacgaaatg atggccgccc cttcgtgggc ggctactact tttcttcttc ttcaaggtgt
   203161 tataatctca caataggggg tgtagttctt ggaacgttat caggagctcg aacttgttgt
   203221 ggacaaaatg gtggaggaat tcacccgact tagaaaggaa aacgaacagc tctggaatca
   203281 actcgagacg tcacagaaaa aagtcgaaga atctgagtta agaatccggg agcttgagct
   203341 ccgccttgag agtgaatcaa gaacaatctc ttcactgata aaaagagtca gaggcagcct
   203401 cgttgaagac agtaaaaaag tataaggagt ccagctatga agcgttcagt ctctctagat
   203461 ctaggaggaa ggaagtacac ttttcttact agtgatccgc aagaactcgt ggatcaagtt
   203521 ttttcaaaga taggcgagct atatgattca ctgaagaata atgaagagga aatcggctat
   203581 gaaaaaatac tggtcgggat ttctgtcaac cttgcacacg accttgtgag aagtcagaat
   203641 gaactgctta ggctaaaggc gaaatacgaa gaggttcttt cagaatactt ccagggacgt
   203701 gacgaggttg agaagtagat taagaattgg agtttttgat tccggaatag gtggcctcac
   203761 tgttcttaaa aggctcgtgg agacgttacc cgaaggagtg agttttcatt attttgccga
   203821 tactgccaga gttccttacg gctcaaagcc agtagagacg ctaagaagat atgtaagaga
   203881 aatattcgac ctctttgctc aattaaatgt cgatgcaata gtgacggcct gtaacacttc
   203941 tgattcgata cttacgcaaa gtgataaagc ggagctcggt gttccatact tcagcataat
   204001 tgatcctaca gttagaagaa tcggcgagtc ggctcccgat aattcaacgg tggccataat
   204061 cgcaactgaa aacacggtaa agaagtctct ttacctcaga aagttatttc gctatgaaag
   204121 cttcagtaag atagttcaaa aagcttgccc actcttcgtg ccgttgatcg aagagggtat
   204181 ctgggaaggt gagattgtcg actcgatcgt caagtattat ttgaatgact ttgctgagat
   204241 tgagccagac ttcatgattc tcggatgcac tcactaccct ttcataaaaa actcgattga
   204301 gaggtttttg ccttcaaaaa ccaaggttgt cgatccatcc gagtatattg tgagggacgt
   204361 agctgactgg gctacgattt tgtcgcctga accttcgacg gttgattatt acgtaagtgg
   204421 caacaccaga atcttccagg atattctaaa gagatatctc ggaagattcg agaatgtgaa
   204481 gggtgtcgat ctgaacgtta cagggataaa tgtgagagga agagtttagg atgagcaaga
   204541 tagttcttgt cggaggtggg accgggctat cgactttcgc tcgagtgata aaggatttcg
   204601 ttacttctct tactctgatt gtggctgtaa cagatgacgg gggaagttcc ggaatattga
   204661 gagaggcttt aggaatgcct ccgcccggtg atgtaagaaa caatattatc gcgctagccg
   204721 atgatgaaga actgttgacg aaggtgtttt ctcacaggtt cagtgccccg gcgatgaacg
   204781 gtcattcgct cggaaacata attatcgctg gtctcatgga gatgtgtgga ggtttttccg
   204841 aggcagtttt gactgcttcc agaatgctta agatcaaagg cattgtcctg cccgtggcgg
   204901 aggatctcgt tagtctcgtg ggtgagcttg acgatgggac aattgtcaag ggagaatccc
   204961 gagtatcggc agctggaaag aggcttaaaa aagtctctct tgacagagcg tgcgaagctc
   205021 ttccagaggt gagcgactcg ttggtttcgg cagatacgat aattatcggt cccggtagtc
   205081 tctttacaag cgttgtacct aacttccttg tttccggtgt aaaggaggcg ctgaagagat
   205141 ctcaagcgag aaagatctat gtctgtaaca tcatgactca accgggtgaa acggatgatt
   205201 tttcactggc aaagcacgta caaatcgcag agagttattg tggggtagaa ttcgatctta
   205261 ttttctggac agagatcggg ggcgttgcat cgtcggtttt gagtaggtat gaagagaaag
   205321 gctcacgtcc ggtcgaaaat gacatgattt cggatgggag ggttaaggta ataaccggag
   205381 cgactaccga gtttatagac gatggaagaa gacgccccgt acttaggcac tctcgagccg
   205441 gaataatttc gattcttcat gaattaggct tgttgggaga ggaaggaatt tgagctatgc
   205501 cgacaaactc aaagaagagt tatgtcatct tccgctcgat gatcaggcag aatgtagagc
   205561 cgagtatctt ggcttcgtta aatcaagggg aactttgaga ttaaggggga acacctcatt
   205621 cttggtgatt cctctcacgt caattacaag tttgaagagg ctgtttcagg tttcaaagag
   205681 actttctatt ccaatttttg aaacccaggt aatcgaagaa gtcagattag ataggaaacg
   205741 cggcggagaa ctgagttatc tctatggaga agttgaagaa tttcttcgaa ggaatggaat
   205801 atctgtccga gatgattcga ttccacgacc tgtcagggag gatccggtct atttcggagc
   205861 ctttcttaga ggcctttatc tggccggtgg atcggtagtc gatccttcta aggagtatca
   205921 tcttgagatt actctcgata caaccgaagc atttgtgaat tcggtgaaac agtatgtttc
   205981 tgagaacttc aacataaaag tcggtgtggt gaaggtcagg gacaaattca aagcatatgt
   206041 gaagtcctcg atggatatca tcgagctact ttccctgatg ggtggaaaga agaccgtcac
   206101 aaggctttcc agcgctatag aagtgagaaa gatcaggagt gatgttagta gaaccctgaa
   206161 cttcttgaca gcaaatgcaa ataagtccgg acaggccatg gcaaaacacg tcaaggccat
   206221 cagaattgtc gacaggaagc taggaataga aaatctggaa gaggaccttc aacaggttgc
   206281 cattctacgt ctggagaacg aggatctcaa tttgagagag ctcggcgaaa tcatgaatcc
   206341 accgatgagc aagtcagcag tatataatag gttgaaaaaa ctaatggcgt tggccgaaga
   206401 gctaggagat aaataaatga aatgtccatt ttgtggtgga gattctacga gagtcctgga
   206461 ttcaagacca accgatgaaa acacttcgat tcggagaaga agagagtgtg agaaatgtgg
   206521 tgctagattc acgacatacg agagatatga aagacttccc tttttggtgg tgaagaaaga
   206581 cggacggaga gagaagttca gcagggagaa actcttgaat ggactgctaa gagcctgtga
   206641 gaagagacca atatctgttg atacagttaa tgaaatcgtt gattccgttg aaaacgaagt
   206701 cgtgaagtcc gggaaacatg aagttgtttc aagcgagatt ggtgagatgg tgatgactaa
   206761 actcaagtct ctggacaggg ttgcgtatgt tagattcgca tctgtttaca aggaatttag
   206821 agatttagac catttcatgg atataattga ggaactcaga gacgacagaa atcgttgaat
   206881 ggaggggtcc aggtcgtgaa gaagaaagtg atctacctta cgcaagaagg cttcaacgga
   206941 atgaaagagg aactggaaag cctcaggaaa aagctaatgt acgatattgc tgaaaggatt
   207001 aaggaagcca gggaactggg cgatctcagc gaaaacagcg aatacgatga ggccaagaat
   207061 gagcagggaa gaatcgacag cagaataaaa cagctggaag agattctcaa caatgccgag
   207121 atcattgagg acgacggcga cctcagcact gtgaagttgg gttatgttgt ggagttactg
   207181 aatatagata cgaatgaaaa atctcaattc agaatcgtta atgctcagga agcaaacata
   207241 tttgaaggga agatctcttc cgattctcct attgggcgcg gagtgttgac gcacaaagtc
   207301 aatgaggtgg taagggtgaa gactcctgcg ggttgggcga aatacaagat tcttaccatc
   207361 ggaaagtgat ggggatatct aatgagtgaa gaaacacgta gacagagaat cgaagagata
   207421 aatcagatgc gtcaggaggg tattgaacct tacccgtata gctttgataa gacgcactgg
   207481 gcagctcaga tcaagagcga gttcgcctat cttcaaaatt cggaaacgaa ggaagataca
   207541 gttcttagta cggccggcag agttgtcgcc gtgagaaatc acggaaaatc gagcttcttt
   207601 gtcttgaggg ataactctga cagaatccag gcatacatca ggcttgacgg agtgggagcg
   207661 gagagcttcg aggttttcaa gaaatacgtt aatattggtg attttgtcgg cgtaaaggga
   207721 tttcccttca agacccacac gggagagata tccgtttttg ttaaggagtt cgaaatactc
   207781 tccaagtcta tcaggatgat gccggagaag tggcacggcg tcaaggacaa ggagataatc
   207841 tacaggcaaa gatacgtaga gatgctttca agtgacgacg cgctgaagag attcaagatt
   207901 cgttccgagc tgttccggct ggtcagagaa tttcttcagt ccaagaattt tgttgaagtg
   207961 gacactccaa tgctccatta tttgaccggg ggtgcgtcag cgagaccatt caagactcat
   208021 atcaacgtct tcgagtcgga tatgtacttg aggatagcgg aagagctctt cctcaaaagg
   208081 tatctggtcg gaggatttga gagaatcttt gaaatcggga agaacttccg aaacgaaggt
   208141 gtctcataca agcatcatcc tgagtttacg atgatggaat tgtactgggc ttacgcggac
   208201 tacaacgaca taatgaacat caccgaagag atgataaatt tcgtggtccg tgagatcact
   208261 ggctcggaga ttatttcata ccagggtaaa gagatcgatt tttcaaggcc ttggcgcaga
   208321 gtgaagatgg cagattttat caaggagaat ctaggcgttg atatcctgga ggactcagat
   208381 gatagaatga tggccgtcct taagaaacac aacacagaac cggagattaa agagagaggc
   208441 catttaatcg aaaaattgtg ggatttggtt gagcataatc ttgtgaatcc cacctttgtt
   208501 accgaacacc cggtaattat ttctccgctt tcaaaggtgc acagagaaga ctcaagagtt
   208561 acagagagat ttgaactgat aataagctcg atggaaatgg ctaatgcttt cagtgagttg
   208621 aatgatccta tagagcagta caaccgtttc ctccatcagg cgggtttaag ggatataggt
   208681 gatgaggaag ctcaaatgat ggatcatgac tttatcagag ccctcgaata cggtatgccc
   208741 cctacgggtg ggcttggagt gggttgggac aggataatta tgctggttac tgattcccca
   208801 tcgatcaggg atataatccc ctttccactt gtaagaccta tctcctttga ggaagaagaa
   208861 gcattagaga taagcgatga acagtagagg tgatttgatg actctttatg atcgcattca
   208921 gcaggatatg aaagaagcaa tgaaggcaag agactctctc aagaccaata cactgagaag
   208981 cgtgatctcg tcggtaaaga tgtatcttgc ctcttctgaa gaagctagat cgggtgagct
   209041 aacggatgag attgtgattg gccttgtaag aaaagaggcg aaaaaacgtg aagagtccat
   209101 cgaagcgtac agaaaggctg aaagagacga tttagtacaa agtgaatccg aagagctcga
   209161 aatacttaag agttatcttc ccgaagaaat gagtcctgat gaaataagag cagtagcact
   209221 aaagacaatc gaggatttga aggcaaacaa cccctccgat ctgggaaagg ttatgagaga
   209281 actgatggtt agattgaagg ggagagtgga tgggaaactc gtcaacaaga ttgtcaggga
   209341 gcttttggag agtagatgaa agtcgagttc ttgactgcag aaatcgggag caccactacg
   209401 gtgctctctg ctttttcatg tgacgcttct ggatcgatga gatttgtggg acagggtgag
   209461 tcctatacga ccgtaaatga gaacgatgtc acaatcggca ttgacaatgc tttgagaaac
   209521 ctgaagagat ctctgggctg cgacaacctc gaatggagtg tcttcctggc ttcctcaagc
   209581 gcggccggcg gtttgagaat gacggttcat ggactggtgt acgacatgac cgtaaaggcg
   209641 gcaagagaag ctgccctggg agctggcgga gtgataaagc tggtaacagc cggcaaattg
   209701 agtgagagag atctaaagaa gattgaggca gccaatccaa agctcatact tcttgcaggt
   209761 ggggtcgatt acggagaaaa agagactgtg gtacataacg ccgagattct tagagatctc
   209821 gatcagaata ttccaataat attcgctggg aatgttgctg ctgccgatga cgttctagaa
   209881 ataatctcga ctaacgggag agacgtccat attgtcgaca atgtttatcc gcgaatagac
   209941 gagctaaacg tcgaaccaac gagacaagtg attcgtgaag tcttctccaa aaatatcgtc
   210001 aatggccccg ggatggaaaa ggtctattca gtcattgaca aaccgataat accgactccc
   210061 gcggccgtca tgctggcgac ggaactcttt tcccaagtca atgacgacgt tattgtggta
   210121 gatatcgggg gagcaaccac cgatgtcgat tccgttacag acggagatcc cgaaatagcg
   210181 tcgatcttgc tggctcccga gccaagatca aagagaacgg ttgaaggtga tcttggaatc
   210241 tatgtaaacg ccagtcatgt aatagagcaa atcggaagag atgaactggg gaaagagttt
   210301 cccgaccttg agaacatcct tcgaaaccta actccttatc caaagaatga tcgtgatgag
   210361 gctttctcct tgaagttggc cgcatactgt ttcgagacct ctatccgaag acacgctgga
   210421 accataaaac agctctatgg tccgactggt agaaccgaag ttgcttatgg gaaggatctg
   210481 accgccgtga aatcggtgat cggcaccgga ggaatcctta gtagatcgaa attctcttcg
   210541 aagattatga agatcgtacg gactctctcc gcgaaacacg aaaaagagtt gttgcctaaa
   210601 tccagtgtaa gattattccg ggacagaaat tacatatttg gcgcagttgg tctgatttcg
   210661 accgtcgatt taccttctgc tagagatctg gttgagaact ctcttgaaga gctgaacacg
   210721 acttgaattc ggtgctcggc taattcgtaa gtccagattg acaagcgttt ccgattgaca
   210781 agcgtttcct cggggtgata gaatctccat gaatgcgggt gtagctcagt tggtagagca
   210841 tcagcttccc aagctgaggg tcgcgggttc gagtcccgtc gcccgctcca attatttgac
   210901 ttcgtatacg gttagaattg cttccccgag ttttcgaaac tcctttaggc tgagatctag
   210961 gtcgcgttcc atagtttctc ccaacccggc gatgcccgtt cttataatcg gttcaaaagt
   211021 aatgtggagt tcattgacca ggcccaaatc cgcaaagtga tcgaaagtct cggcgccgcc
   211081 aatcaagcac acctcatcgt gccccttttt ctccaaatct tcgagaagtt cttttggggt
   211141 tgaggatgtg aagatgaggt tttcggatgg tctataatag tctggatttc tcgtcaatac
   211201 gatgttgagc ctgtcaggaa gggccctccc gatagcttcg tgggtcttcc tccccattat
   211261 cactatgccg acacttgagg tcagggattt gaagtacctt ttgtcttctc tcgatgtcca
   211321 ctctgtctta tcgtcttctg ataatgagat ctttccattc aacgtacttg caaaaaccgc
   211381 aatgactttc actttgagca cctcccagtt gacgatgtcc aatgatcttc attttatctt
   211441 tttttgactc tccgaacaat gccagagagt cagtttgatc aagatatgtt attcttcttt
   211501 agatgggaga gtgaaagaga ttaagaggat tgcctggaga gtcttgattt tcgctatgct
   211561 tctcatgttg atttcttgta gtaacagcac aaattacgcg gtctcgataa agcagattga
   211621 ctgcgaaggt tcaacctgta atttgaggct ggagatttcc gaagacgggt taagtcttcc
   211681 ggaattcctt atcactttgg atcgccctcc agtcgacgga agtgatggaa ttagaatcaa
   211741 aggaacagag atttctttag atctgaagga ggcggggagc cacacgattt ttgtcacttt
   211801 ggtcgatggt aagagattct taagcacccc ggtttccaaa gaagtaatta taaatctgaa
   211861 tcctcccgat gcacctgatc tcacttggag cctttctcaa ggtgagctca aactgtctct
   211921 tcgaagcgat agttccgatt cggtcactga ctatcgcatt cttgtcaaca acgtcttgca
   211981 cagctcgacc caaggaacat tctcatttcc cgttgatcgg gatgcccact atgttattca
   212041 ggccttttcg gtggctggca atctaagatc cgacccctca attcttgatc tcaagctttc
   212101 ggaggatttt gcccccgaag tagagcttac ttctgtgctt ccttttacga atggcgcagt
   212161 cgatattact gtcgtcgatg actgggatag aacaaacgat ctttctataa aggccttcct
   212221 tgaggagagt aacattcttc tgctttttga tggaaacagg ttgattcctg agatacccct
   212281 tcccgagggt ataaatacac ttgttgttcg ggtcgccgat tcatcgggaa acgtgaccga
   212341 gttaaagagg gagctgaaaa cagtccgaaa gacctcggtt gaaattcctg aactgtacat
   212401 cgaggaaggc actattagga acgcaagatg gacgacgcca aagggcatgg ttgaacttca
   212461 gagatacatt gatggcgaat ggataactct gtctacccac agttcggaag aggagtcatc
   212521 aataatcgca aaggacagca tttctgaaaa aggcgatctt taccgaattg ttgtggactc
   212581 tgataatgag aaactgctgc cttcagttcc ggtttttgca aaggaaagtc tgtttaggag
   212641 atttagcacg gagttcatat cgtcttttct cggaatggat gcgctgctag tcggcggcaa
   212701 ttcctacaga ttttacggaa acatcgtcgt tcgagaaaac accgtcatga gagttgaatc
   212761 gggcacgtct ctgacgattc cccgaggaaa cactcttcta gttagcggtg ttcttgatct
   212821 ggaaggctgg aaggatagaa taaaagtgaa tcccggcggt tcctcgggaa caattgaggt
   212881 aacaaataac ggagttctta ttgccaggag cgttgatttc aataattcca ctgtatcgat
   212941 tgaaaatgct gccgttgcgg tgtttgagga ttgcacgctg tcaggagacc tgaagattag
   213001 cggagccaga acggttcaaa tatacaacag ccagttcagc ggagatatgg agatcgagaa
   213061 cgttagagag ctgctggttc atcaaagcga tttttcggga agcagtctgg ttctgagtgg
   213121 tttgggtagg gttttgcttt ctagaagctc tttacgattc cattctctcg agatagtaga
   213181 ttcgagactt gaagcgtatg aatcgctttt cgaagctgaa ggtttgtttg ttcgaaagac
   213241 atctcatctg ttgcagacaa gaggtgcctt gatagaaact gaaatctccg tttccggtgc
   213301 atcatctatc catatcgaag aacccaattt gaccggagat gtcaagatag agctcagaga
   213361 cctttcaagg ctgagtctgc agaaggattt cgaaggatcg atcgagattg tttcggatga
   213421 gaagtcggca ataaaggtat tttgaaatgg gaaagaagtt tcgcgatttt gatccttacg
   213481 ttctagaggc ttttgaattc gatttctctt ctagacacgc gcgcataaac catgggacgg
   213541 ttttgctatc ctggttcgtc gaacccccca aaggttcgag aagagcgctt gaactgggtg
   213601 caggcagtgg cgtgatttcc atatatttgg caaaaaactt cggacttgaa gtgacgggag
   213661 tcgaagttga tagagaattg cacgctgttg cgacaaagaa tgcttcactg aacggagttg
   213721 aaaatctcac aagatttgtg aactgcgaca tagacgatta tgcggcaaac aacaggggag
   213781 aggtgttcga tatggtagtc tcgaatcctc ctcattttct tcattccggt gtagagagtc
   213841 ccgatggcaa aagaaatagg gcgaggagaa tcgaccctga actggctatg gtcttcgctt
   213901 ccgcaacaag taagttgctc aaaaatagag gagctttttt ctttttactc catccgaggg
   213961 atctaacgaa gtgggtccgc ctgcttgagg attttaagct tggaatccat aaaatgcgtt
   214021 tcgcacatgg gtcaatggaa ggacaggcac agttggtatt agtgagtggg agaaagagtt
   214081 catcttctga aatcgttgta gaaccaccca taaggatgag gtgaagttat gttcatcagt
   214141 gtagaaggaa ttgatggatg cggtaagacg actcagataa aacttttagc ggagtatttg
   214201 agtcaattag atgtagaatt cataattgtc agggaacccg gaggaacaaa ggccggtgaa
   214261 gagataaggg acatacttct ccataaggag tatcatttgt tctctgagtc tgagcttcta
   214321 ctctttatgg cggcacgtgc gcagatagtt agggaggtca taaggcccga gcttgagaaa
   214381 ggcaaaacgg tgattgctga tagattcatg gattcatcgg ttgcctatca gggagtgggt
   214441 agacgactgg gcagagagat agttcaagct atgaacgcat ttgcggtagg gggaaccgtt
   214501 cccgatatta ccttttacat tgatgtaccg accgatgtag ctatttctcg gatgagaaaa
   214561 gaaaagaaaa atgataaaat agagatggag aattttgatt tcttcgaaag agtgagagaa
   214621 ggatatgaga aactgatggt agagaatcgt gagaggtttg ttcggataga tggaaaacaa
   214681 tctgaagaga aagttcaaac tgatatcagg aagtcgttgc gggttttgtt tcataagaaa
   214741 aatcaaaatg ggaggtgact gaaatgtact tcgacaagtt ttctgaaggt gctgcgcagg
   214801 tcttcgtgac agcccaggac gaggcgagaa gtctcggcca cccttatgtt ggaactgaac
   214861 acctccttct ggcgatcatt aaagtagaaa atgagctaac aactaggatt ctgagaaact
   214921 actcgattac atacgagaga atctccagag aagtcacttc gatggttggg acaaacgttc
   214981 atcaatctgt agtaggtgcc ccgcaaatga ctcccagagc acgtagagtc attgagctcg
   215041 caaatgatga atctaagatg ctggggcaag acaagattga tgtagaacac ataatgctcg
   215101 gaatcgttcg cgaaggagag ggcatagctg cccacatatt gaaacagatg ggagtcaact
   215161 tatctcagct gagaagggag atcattgaga acataacagg ggactgggaa gaaggtggag
   215221 aacagcccga gaatgttttt gttcaaccga gctctacaat gggccccgcc attaagcaac
   215281 ttgaaggttt tggcatcgat ctcactgcca tggcaaaggc gggtaaactt gatccgatag
   215341 ttggcaggga gattgaaagg cagagattga tggaggttct gtcaaggaga aagaagaaca
   215401 accctgtctt gatcggtgag cccggagttg ggaagaccgc tattgttgaa ggcctggcgc
   215461 agatgatcgt agatgaagag gttcctgaaa cgcttaagaa taaggtaata ttcgctcttg
   215521 acgttgcttc tctcatagcc ggaactaagt atagaggtga atttgagaag agactgaaga
   215581 aactgattca cgtggtcaag acaaatggaa acataattct ctttatcgat gaggtacaca
   215641 cgattgttgg agcaggctct gcagaaggtg cggtagatgc tgctaatatt ctgaaaccgg
   215701 ctcttgcgag cggagaaata aggtgcatag gttcaaccac tcccgatgaa tacagaaagt
   215761 acatagaaaa ggatgccgca ctggagagaa gatttcaaaa gatatatgtg actgaaccta
   215821 ctccagaaga gtctgtggag attctgaaag ggattagaca taaatatgag ctccaccaca
   215881 aagtgcacta tacggaaaag ggcttagagg cagcggtgac tctatcgcac agatatgtgt
   215941 ctgatagatt cctgccggac aaggccatag atatcattga tgaggccggc tccagagctc
   216001 ggttgaagaa gctcactata ccggatgagc ttaagaaaat gcagggtgat attgagtctc
   216061 tcaaaatcaa gagagaaact gctgcagccg atcaggagta tgaaaaggcc gctcaactca
   216121 aagaaaagga gcgtttaata cagcaaaagt atagagatgg gtacaacgat tggaggaaca
   216181 gagtggactc tgagatcgtc acgctcgatg atgacgaagt tgccgaaata gtttccaact
   216241 ggaccggaat tccgcttagg aagctcgagg aaactgacag cgaaaagctg cttactcttg
   216301 agaacgctct tcatgagaga attgtatctc aagatgaggc catctccgca gttgccaagt
   216361 cgataaggcg cgcaagaagc gggctaaagg atccccgtag accgattggt acctttttgt
   216421 tcctcggacc gacgggggtc ggaaagaccg agttggcgaa ggcccttgcg gaatatctct
   216481 ttggcgatga taaggccctc gtgagatttg atatgtcgga atacatggag agattctcgg
   216541 tctccagact aattggagct ccaccaggtt atgtcggtta tgatgaaggc ggtacgttga
   216601 ctgagagaat caggaagagg ccgttttcag taatcctgtt cgatgagatt gaaaaggctc
   216661 atcctgatat cttcaatatt ttgcttcaga taatggacga cggacggctc accgattccc
   216721 aggggagaca ggtagacttc agaaacacca tagtcataat gacaagcaac ctgggcgggg
   216781 agttcataaa caagacaaag tcatcaatcg gatttgtaga gtcgagtcac gaagaaaaag
   216841 aatacgaagc gatgaagacc tccgttctag aggaggtaaa acgtactttc aggccggaat
   216901 ttctgaacag gctcgatgaa gttgtggttt tccatcagct cacgaaggat caaataaaga
   216961 cgatcatcga tattctcatg aacgacatga gaaagagact gaatgagaag catctgaagc
   217021 tgatactctc agaggccgcc caggagttcc tcgttgaaga aggattcaat cccgtgtatg
   217081 gtgcccggcc tctaaaaaga gccatacaga agtatgttga agaccctctt tccgaagaac
   217141 tgcttagagg gagatttcag gacggtgatc tgattacggc cgaagttgac aatagggttc
   217201 tgatcttcaa gaagaaaaaa gacgagaaga ctgaagtgaa ggccatcacc tagattcatt
   217261 gattggcgag gtttcggggg gcactagtgg cccccttttt taaaatacta acggaggtgt
   217321 atctccgtat attacggagt cgttctagat gaagaagaaa agaagctatg tctgcgatgt
   217381 gtgcggctat gaatcgcccg cttggtttgg tcgttgccct tcatgccatg agtggaacac
   217441 tgccatatcg tttgttagcg aaggagacaa ggacgaaaac atttctgttg cagatataga
   217501 agtaagaccg ctttcgcaag ttgacacgac cgaatcatct cgaattgaca cgaaaattga
   217561 agagctcaat agatcacttg gaggtggagt cgttccggga agcgcggttc tgatcagcgg
   217621 agaacctggt ataggcaaat cgacactcat gctccagctc tcggcgctga tcgccggaga
   217681 aaagcccgtc ctgtatatat cggcagagga atctgcgcgt cagctaagat tgagagcgga
   217741 gaggctcaaa atccaaagga aggagcagat agaagtagta tcggctaccg agatagactt
   217801 cctgggatct ttggccattg aaaagtacgg catgatcgtc gtcgactcgc tgcagacaat
   217861 aaagatgtct tctgtacctt ctttgcctgg ttctgtagtg caggtcaggg agtgcgctaa
   217921 catgttggtt tctcttgcaa aatcctctga cattcccgtc ctgattgtgt cacacattac
   217981 aaagggaggt caaatcgccg gcccaaaact tgtggagcat gttgtagata cggttttgta
   218041 cttcgaatct agtaaaaccg gcttcagatt cttgagaacc attaagaaca gatttgggcc
   218101 ttcagacgaa attgccgtct tcgaaatgac ttcggagggt ctcaaagaag ttcccgacat
   218161 cagcagtctg tttgttgagg actacattag cgcccccgga aatgtcgttt cggcaatcta
   218221 tgagggttct cgagctatgt tgatagaggt gcaggcatta gtttcaaaac ccgtttatgg
   218281 tacaccaagg agactcgcct cggggatacc tcttgaaagg cttcttcttg tgaccgcggt
   218341 gctgacaaaa agagcgggaa ttcctcttga cagcctagat ctctatatga acgttgcggg
   218401 aggactgcag atagaagata ctggcgtcga tctggcgatc gcagcggctc tgatttcatc
   218461 ttatacagat acttcaatac ccgaaggtgt agctttcttc ggtgagatag gtcttgacgg
   218521 aaccgtgcgc tcagtttctt ctatggagaa aagaatcgag agggcgaaaa aatcatcctt
   218581 catgaggata gttgcaccgg aaggaagaaa tggtataaaa gatgtaaagc aactcttaaa
   218641 attcatcgga ggtatgaaga gtggaaagga gtgaactgat agaaaaaatc aagaaaattg
   218701 ccccgggaac gcccctccgt cacgctcttg atgatattca agatgccaac gtcggcggat
   218761 tgctgttttt tgttgacgac tacaagaaat acagtcattt aatgcagata ggattccttc
   218821 taaactgtca gttcaatccg aacaaactct atgaactggc aaagatggac ggggccattg
   218881 tcgtcgatga cgaccttact acaatcgttg gcgcaaacgt ccagttgata cccgatcaca
   218941 cgattcctac aaatcagact gggatgagac accgggctgc agagaggatg gccaaagaaa
   219001 caggtaggat gcttgttgca atttcaaaga gaagaaatac gatgacgatc tttctagggg
   219061 acttcgccta cactctgaac aatgtggaga ctctcacttc aaagatcact cagacgataa
   219121 gaacggcaga gaagtacaaa gaggcattca tcgaggcttt acagggtata gagttcatgg
   219181 aacgggccgg gtctcttact ctttacgacg ttgtgaagac aatccagaaa ggactcatgg
   219241 ttgctcttat ctgtgaggaa gcggaattca cgatagccga gctgggcaac cacggaagat
   219301 ttccttcaat gcagcttacg gagatcctcg tcaatgttaa tgaggagatc gagagtttaa
   219361 tcctggactt ccacgaagag ttactggatc aagatagcct ggaggaagtc ttcgacaaga
   219421 tgaggagtct cggagagaag gagataagca gtttcggagt gatattgaga aatctgggtt
   219481 acgatataaa taccgcttct caagccgttg aatacttcat ccggtcgagg ggtattcgtt
   219541 ttgccagaca cattcccaga attcctttgc aggtcgccat caatttgggc aaggagtttg
   219601 gcacattttc gaatctcctt gaaagcagcg aagacgagct caaagatgtc gacggaatcg
   219661 gtgagaaacg agcctcagca ataagacatg ctatagatgt ctacaaaaaa tcgggagaaa
   219721 tcacagttcc aggcttctaa gctgcttcga tatttcccta agctccttct tcgagatccg
   219781 ttgaggcact cccttgtcaa agatcagctc tccggaattc cagactttct caaacgatgt
   219841 atcgaaagag gacttgagaa ccagctgaag ggttatcggc aaattcatat cgaatttctc
   219901 cagatcaagc gatgatttcg ccagaacaag atctgctgag taaccctctt tgagtactcc
   219961 agaatgtttc ccaaaaaacc tctgaaagag tttgtaatta ttgtcaaata gcgtcttcct
   220021 tatctcgtag tcgataattg aattgtcaaa acctctatgg caacgttcag tgagaattat
   220081 ctcttttgct tccccgagga tgtccgcccc gagaaatccc gtgccgaggc agacctgcat
   220141 acctcgacct agcaaatctg agatattcgg ctgaaaggtc ccggcataca tttccgatct
   220201 caaagacttt gtgacgaaaa tccttcgaga agagattatg tccatatctg tctcactgag
   220261 gttcccgccg tagacaacac cacattcggg gatcaatatt ccattcttca taagcctctc
   220321 tacaaggctt tctccccacc tgaagaggca cacttcattc tcccgcttca tgtcgaagat
   220381 gagcagattc agattcatcc ccttctcata aagacctcgt agcttttgaa tagcttcctc
   220441 ttcaaaggtt gcaagattgc taacataaag catcggacag aacgaggcgt ctctggtaac
   220501 gttttcccaa cgaccaccga tctcgtccag cgtgtcttca gtcagaaccg gcccgaccgc
   220561 aagatagaca ccgtacttcg cagcaatctc cctatatagc tccggatcca catcgggatg
   220621 atcaacaacc ggaccgctca tggcgactga accgcttcta attgccttca ttacggctaa
   220681 ctcagccgaa agtcgaacca gttcggaatt cacctctttc cttcccgaat taagcaagcc
   220741 accgaaaaaa tcgatctcag ttctctcgcc tttcaggact ctgactgggt atggagaaat
   220801 gctggaatag atgctgtaat gagcattgaa aaatgagggc aacagaatgc ctttttcgcc
   220861 atcgactatc tcttcatctt cctggatgct gtattctctt cttggaccaa cgaaagtgat
   220921 ttccccgtcc tcaaatctaa ccaaagcatc atccacaaga gggttcttta gatcatttgt
   220981 gaatgccaaa acgttagtta cgatcatctg gaaccacctc ttcttgattc taaataagat
   221041 ctgataacgt aagtctcgaa actaaagagc ttgggaataa ttttgagccg ctttccaggt
   221101 tctcgaacca ctctgtaaag ccattcaagg ccggctcgtt gcatccataa aggagccctc
   221161 ttaagattgc cagaaacgac gtcgaaagat ccgcctacgc ccatcaatat ctttgcctgg
   221221 agtttctcag cattctctgc aatccaaagt tcctgtcttg ggacacccat cccgacaaac
   221281 aaaatatctg cagatgaagc gtttatttcc gaaacgattg tatcattttc ttcttgcgtg
   221341 aagaagccac tgtgatgccc gacaatgtca attagtggaa atctttgttt tagtttttcg
   221401 gttgtctttg ttacaacagt ctctttagat cccagaagaa atatgctcaa tcccttttca
   221461 gcagagagtt cacagagctt aagcatcaaa tcgatgcccg gaactctttc aaggttcctt
   221521 ttggaaatta gcttaattgc tttcagtaca ccgcttccgt caggaacgac gtaattcact
   221581 ttgtgtagag ccctgcaata ctcctcgttg tgcatgtatt cgtaagcaat caaagcgttt
   221641 atagtaagga cgtgtgatct aactcccgat tgtgttcgct gaatgagttc atttgctaca
   221701 gaaactagat ccttctcttc cagttcgaaa tctaagaatc tcaaacgaac acctccaaat
   221761 ttcattaaaa cacgaagagc cacattttca acgaaaagag gaaggcttag ccttcctctg
   221821 gtgccgaggg cgggaatcga acccgcacgg ggctctcaac cccagcggat tttgagtccg
   221881 ccgcgtctgc cagttccacc acctcggcca gcaataaaac tttaccatca aaggttatca
   221941 gtgtcaacga gtttctttcg gatacgccac cagtcagaac ggtcggcttt acagcagcca
   222001 gaacgctttc ctattgtttc agcaattcgc ccagcgagaa gcagaactcc ggcattcctg
   222061 ctgcccaggg acctatctcg tatggctgga agtatacgca cagtcttcct gccttcaggt
   222121 agaaaccctg gtcggttgtg acccccttga aatattccgg ccacaggtct tctcgctttc
   222181 caatctctcc tgttatcagt tcattcagtc tgtcttcagc ggccgcactg tccaaaacac
   222241 tgtgaagggc aacaaggcgg catctgtcaa cagagatgtt ataagttctt ctggaagcac
   222301 cggggtgggc cccgcctgtg tactcgctga agatcatgtc gatgctaaga atgccatcgc
   222361 tgagataagc ctcatataaa acatatatgc tgaattttcg gaatggccat tcggaagttt
   222421 tcgattcttc gtaagcggcc tgggccattt cttcgatctg agttctataa tccgtaatct
   222481 cagactcaat agttgcgtta agatactctt caaaaggctt gtctttcaaa ccgcttattg
   222541 aaggaatctg aatatttatt tcgcacactt cagagtcttc gacaatgttt ttcccgagaa
   222601 cggatatcat gtttccattg gaaagaaaga tctgttcagg aagatcctcg gtataagaaa
   222661 acgcaaccgc aagagcgaac accaagtaga ctgcaatcaa caatcttcgc atagaaaccc
   222721 ctccttttaa cgatcattca tattgtaaat gctacaaagg acagaataag catcttcata
   222781 gaagaataat accatcgagc taccgctggg tgagccaaaa agattgtgca attctttctc
   222841 tgatactaat cacatcattt tactcaaaat gaatcaagta aatgggtctt gtgcgattct
   222901 gtcattgatt tcagaggttc gccattttca gagcgactgg cttgtttgga gaagtaaggc
   222961 agaaaaacgg tatcgcggtg atatgatttg taagggggtg atagatttga ttccagagat
   223021 aaccctggat atggttaggg taaccgaagc ggcggccctg ttttcgagtg tcttcctggg
   223081 gcgaggtgac aaagaagctg tggatcaaag agccgttgat gccatgagag gaattcttga
   223141 tctgctggac atgaagggta cagtcgttat cggagaggga gaaaaggatc aggctccgat
   223201 gttggctgcg ggagagcacg tgggtaagtg gaacgaagat gatcccgagg ttcttatcgc
   223261 tgtcgatcca gtcgacggga ccagactagt tgcaaatggc cgatcgaacg cgttgagcgt
   223321 tattgctgcg actgaagacg gaaagatcgc tcctctccca acttactacg tagacaaact
   223381 ggccgtcggg aaagaacttg cagggaatct ggatattgaa gctaccccgc gggaaaacct
   223441 tcgtattgca gccgccattc ttggtgtcaa tgtatcggag ctcacagtaa cgatactaga
   223501 ccgagagaga aaccatccgc tcgtggaagc agttagaacg gtcggtgcca gaatcaggct
   223561 tataaatgac ggggatattg ctgccgcaat cgctaccgct cttccggatg gagggacaga
   223621 aatctacatg ggaataggag gatcgcctga agcagttctt gcagccgccg cacttcagtg
   223681 catgggaggc gaaattcagg ttaagtgctg gccaagagac aaagacgaaa ttgaaaaagt
   223741 agaaaaatct tcttttgaac tccacgctgt ttacgggaca aaggatctca tagatggcga
   223801 agaagtagtt ttcagtgcga cagggatcac tgatggctct ttccttaagg gagtccggtt
   223861 cactcacagt caagcaatta ctgattcaat agcaatgaga tcgagcacga gaacagtaag
   223921 aagaataagg gcttatcatg agatagctta caaaaccata acgacgaagt ctgggggaga
   223981 ggtctccata tttaacagcc tgcttgggaa gagagcatga aagtctgaca aataaaaccc
   224041 ttgatgattt gggatttttg actaatagat cccacagcgc tttgaaagct gtagattcat
   224101 tgctgtcact ggaaatgcct gattaagaaa gccacgccgt ctattccggg gcgttatcag
   224161 gttgagaaaa tctgcggcag gagaagcatg gagttccgat ttttcatcaa ttaagtatct
   224221 ttcagcgttt cgaaattgaa tcgtgcacgt ttccacccaa aaatcttacc ggaaactgca
   224281 aagagcggat attggatccc gaaaagtgag cgagaggttt ttatccatat atttgaatag
   224341 tcaatcttaa aaaatctgat cttttagagc ttccaacaaa agtcataatt gaagatcttt
   224401 tgccacaaat gttgttgctc ttccgataag gtctactgct tttcaggtgc tataataagc
   224461 gcaaaacaac ggaggcattt ctgtgatctg cattctgcaa aacgacagtg atttttacaa
   224521 agaaatctcc cctgttgaaa aggccctgga taacacgggg tatgagtatt cgacatggag
   224581 aacctttctt gacgggtttc ctgaggacga gttgagctgt gacggattga taataactgg
   224641 tggtttctca atgagcaact acttcgaagg aagagcacta tcttctggag gaaacttcgt
   224701 tcgtagtttt agggggccaa ttctgggtat ttgcctggga atgcagattc tggccaggat
   224761 cggtggtgag tcactggtcg tatctaaaga gctgggagtt acaaagataa gactggacgc
   224821 tttacatgaa cttttttcgg gaattgaaag tgaagttagc gcctatcaga gacacaatta
   224881 tggtctgcct tacgtacctt atggctacag gcagattgca tggggggatg aaactttcat
   224941 tcagggaata tcttctacag atgggttgag attcggtata cagtttcatc cagaggaaat
   225001 tgagcttggt tctgacgagc agctgcaaag aatctttgcc aatttttgcc ggatcgtcag
   225061 ctttcacaag cgatgactca ggaggggaat atgagagtag ggattatcgg tgtaggaaac
   225121 ataggtggaa tctttgccga caggcttctc ggagagcttg agggtctcga gaagctgtac
   225181 ttgctggaca gaaatcagga aaggctttca aattattctg gcgaaactga gagagtggtt
   225241 atcgcgaaga ctcccggtga agtcctggag aattgcaccc acgtaataat ttcggtgaaa
   225301 cctcaggact gtatggagtt atttgcggaa gtcataaacg gtaagaactc ctctccaatc
   225361 attatcagca caataactgg aattgagata gctttgattt caaggaagac gggcttggaa
   225421 aagattgccc gaattatgcc aaacgttcca agcgcaatag gacggggcgt cacgggattc
   225481 gtttgctcga gttcacttac aggaagggag aaaaaagagg tcgagagaat ccttcgcaca
   225541 atgggtgaga ttgtggaagt cagggagaaa gacctctccg ctgttaccgc tctaagcggt
   225601 agtggcccgg cattcgtgtt tgtgattgtt gagtcattga ttgatgtggg cctaaaaatg
   225661 ggtctctctt atgacgttgc cagagagctt attctcggca ctcttggggg ttcggtcgag
   225721 ttgcttaagg tgaagggaaa tcaccctggc gagttccgtc atctcgtaac ctcacccgga
   225781 ggcacgacga tcgaaggaat ctattctttg gagagagaag gattgagagg cacgataatg
   225841 aaggctctct ttgacaccta tttgagagct gaagagatca actccgagct ggaggtgaca
   225901 cataatggat cttctgaata agggagagag agttaagacc gcttcgagaa agctcaggtt
   225961 gctgagcacc aatgagaaga acgaagcgat catgttgatc tcccgagaac tcgaaactca
   226021 gaaaggagag atcctccggg ccaattccat tgacgttgaa aactcgagaa agaagggaat
   226081 gaaggagtct ctaattgaca gactagctct ttccgaagaa cgaatcgcta tgatggttaa
   226141 atcatgcgag aatgttgcct ctctgcctga tccggttggc gaaatagaaa gcgcttgggt
   226201 tcagaaagat gggctgctta tttccaaaga gagagttccg attggggcaa tcggtatgat
   226261 atatgagtcg aggccgaatg tcaccgtcga cgcttccata ctcgccataa aatccggcaa
   226321 ttcggtcctt ctaaaaggag ggagcgatgc tattaactcc aacaaggcaa tagtttccgc
   226381 aattaagact gccctagctt tggccggtct tcccgatagt tcggtggaac tcgttgaaga
   226441 tacttctcat gaagctgtgc aagagatgct gaaaatgaat caatatctat ctttgataat
   226501 acccaggggc ggcgcgggac tgataaagtt cgtggtcaac aattcaagaa tacccgtaat
   226561 agagactgga actggtaact gccacatctt cgtggactac aatgcagatc tgaataaagc
   226621 ggtggaaata gttgacaatg cgaagacaca gagaccagga acgtgcaacg ccgttgaaac
   226681 gttgcttgtt cacagggaca tagctgcaga gttcattcca agagtctccg aagttctctc
   226741 aaaaaaagga gtggagctga ggggctgtga tgaatcaacc aaatactgga agatgatttc
   226801 cgccaccgat gaggatttcg ccactgagtt tcttgatctg gtgcttgcca taaaggtagt
   226861 taaagacgtg gatgaagcga ttatgcatat tgagaaatac tcgtcggggc actccgaagc
   226921 aatcttgacc aacgactacg ctaattccag aaaattcttg aaagacgtgg attccgcagc
   226981 cgtttacgtt aacgcttcaa caaggtttac cgatggcgga gaattcggct ttggcagtga
   227041 aataggcata agcacacaga agctgcacgc aagaggacct tttggtctta aggagctaac
   227101 aagttacaag tatacgatac ttgggaataa tcaggtgaga aagtgatgtc tagagtggtc
   227161 ctgaaagctg gttccaacct gcttgtaaag agtaacggag aaatcgataa gaaatatgtg
   227221 actgagcttg cgagagagat ctctttgctg aaatcgtcag gcagccaggt tgtcttagtg
   227281 tcttcaggag ccagagcggc tggctttggt tatctggagg gaaaaaagtc gaatggcgat
   227341 ctttacatga agcaagccct ttgcgccgca gggcaggtcc agctgatgaa gctgtatgag
   227401 acggttttcg atctctttgg agagagaatc gcacagattc tagttaacag agacgatttt
   227461 ggagacagaa agagatttct caatctcaga aatactctta tagggcttct tgaactcggt
   227521 cttgttccta tagtgaacga aaacgacaca acctccaccg aagagatcat gttcggggac
   227581 aacgacattc tcgcttcaat gtttgctatc ggatggcagg ctaacctgtt gatactcatg
   227641 tcaactatag atggaatcct cgatgaggaa ggaaaagtga ttcgggaatt tgacccatcg
   227701 aagagactca agaacataga gaataattcc tggggtacag gcggaataga tacaaagata
   227761 agggctgcac gcgccgcgtc tgcaagtggc attagaagtt gtatttgtaa tggaagggat
   227821 ttccgaaaga tagaggaatt tgttcaggga aaggatgtcg gaacaatctt ctccccaata
   227881 gaatctcccg gcgcccgcaa gacgtggatt ggctttcttt caactccgag aggaaagatc
   227941 acagtaaatc gaggagccga aggggctatt gctcgaggga aaagtctttt ggcggtaggc
   228001 gtagaagatg tttctggaga gttctctgaa ggtgatgtag taagtatttg ctcagtcgag
   228061 ggaaaggaag tcgcgagggg aatctctaat ttcagtgctt cagaagtcca gaaaatcaag
   228121 ggagtgagga gcgataggat ttcctctgtc ctgggccatg attgtgcaaa ggtagtggtt
   228181 cacatcgata attcctatcg tatttaacac gttaataaaa gacctcgctt cgtaagatgt
   228241 cgaatggtca aataaatact acatgaaatt tgtggaattg cgaacgcagc cacaatttag
   228301 cagagactat ggataagaac caggtcaaac gaaatctgaa atatgtcttc cttagttaaa
   228361 gcctaccaca gtaatctccg ccctgatgtc taaagatctc gtagtatact tttcgaagga
   228421 ggacacgttg gaggtgaaat atgtatagtt tcccggacct tttgtactcg gatgtcagaa
   228481 tcgaggatgt ttcgaaaagc gatattgtgg tgacacttgg aagaacagac aatatgaaag
   228541 agcagaggta ctcggcggct ttcatcaggg tgtttgatgg agagagatgg tacttcagcg
   228601 cgacgacaga tcttgattca attcaggacg aacttgacag gctggcttct cttgcaaaga
   228661 agaatcgctc cattgaagag catggagtgg tacggaaact ccaggcaaac aaagggtcgt
   228721 acttgagatt cgatggagac ttaaacgttt ttaaaacctc tctcaatgag aaatacgata
   228781 ttctttcaag ttactttccc catgtcgaaa agagcgagct agtgaagttc tggagaggtc
   228841 agtatataga tcagagagtt ggcaagaggt tcttttcgag caagggggcg aatctggaat
   228901 tcgattacca acgagtaggt ttcagactgc tcttccagat ggccaaagat gaagagcagt
   228961 tcatggaaag atatgatctt gccggtaacg agcttgactc tataaaggga cttcatgaag
   229021 aagtgagaaa gactgttgag acctctgaag atttcataag aaatgcaaga ccggttgttt
   229081 cgggcagtta tccggttatt ctctctccgg aagctgccgg cgtctttgcg catgagagtt
   229141 ttggacacaa gagcgaggcg gatttcatga tcggagacag gacaatgatg gaagaatgga
   229201 aaatgggcag aagagtggga agcgaaattt tgtctattgt cgacgatggg aataagccag
   229261 gtgtcgggaa tgtcattttt gatgacgagg gaacaaaggc agaagaaacc tatttggtga
   229321 gagagggcct tctatctgga cggttgcaca gtgcagaaac tgcggcagct ttaggcgaag
   229381 aactgacggg aaatgccagg gctgtgaatt tcgagtatga accgattgtg agaatgacca
   229441 ccacttttat ttcacctggt gaattatcct ttgatgagct tctttccggt ataaaagaag
   229501 gcgtctacgt tgattctcta aaccacggct ccggcatgtc gaccttcacg ctcgctccat
   229561 gtagagcata catggtcaga aacggaaaga ttgcagaacc ggtaaagatc tctgtggtga
   229621 cgggaagtgt tttccaaacc cttaacgaga tcgagggact gaccgaggag ttcaagcttc
   229681 tatcgtttgc gcttggaggg tgtgggaaaa tggagcaata cccccttccc gttggattcg
   229741 ggggaccata tgtccgagtc cgctcgctga gtgttcacta ggaggtgctg gatatgacga
   229801 aggaaagata tgttgttcat tatacagaaa atgcagtaaa gatagttcaa acacaggtca
   229861 gttctgtaag gaagaaagac attcttcgca cgtcgattcg actatacgat ggcaagtcaa
   229921 tgggggttgc cggtgcgctt ggagagtgta atgaagaaga actcgtggca aaggcaaaag
   229981 aatcgttgaa gcttgcgatt ccttatccct acgaggcttc tagagagctc gtgagagaag
   230041 aacgccaccg gtcagatctc cagggcgaag aagaattgct tgaagctaca gaagagattc
   230101 tccagaaact gagaagagac caacctcatt ttagttttag ccacttgttc aaatctgcaa
   230161 gagtcgagtc ttccatgcag aacgatctcg gtcttaactt gaaatcatct cgcgaattca
   230221 taagtctcga actagtcata aaggacaaga aatcatcgaa catcatcgat gcgatgacgg
   230281 gatacgaagg aaggaagttc cataaagact cctttatcga gctgactaat atgatttgtg
   230341 atgcatacat gaaccaattg cctgacatag aagaaggagt ttatccggta gttttcaggt
   230401 cagatgatat gacttactac aagaaactct acgagtctct aaacggtatg cttttcggct
   230461 cgggtggatc ccttctttcg gggaagatcg gcaaaaaagt cttctccgac aaagtcactc
   230521 ttttccagac gcgcaatgtg gaagatggtt tctttgaacc cttttttgac aaggaaggaa
   230581 ccgtgaacga ctccgataga tttgcgttga tagaggacgg agtagtaatc tctccatata
   230641 cggacaaaaa gactgctgcc accttcaatc ttacccatac cggttctgca ggaggggagt
   230701 acgatggcct gcctgagtta gggatgccga tgctcaaatt cgcagaatcg cacaaaaccg
   230761 caaagcaact tcttgacggt cagaaggcca tattcatttt aatcgcgagc ggaggtgact
   230821 tcactcagga cggtcgattc gcgaccccag cacaattggc ctttctcttt gatggagaca
   230881 gattcattgg aaggcttccg gaactgagct taagttcaca catcttcgac atgctgggca
   230941 aggacttccg tggcgtttca agtgaagact ttcttggaat tgagaacgcg aaacttacga
   231001 tcatggacat gaatgtgaag aagctctaga tttccgttcc acgaatacca tgaagcctgt
   231061 attcttcaaa caactggctt gacataacgg aaaatcccgg gagatacggt ttgcactcta
   231121 tagcgatact tggatcgggg attatgggaa agtccatgcg ggggcctaca gggccatcga
   231181 taaaagaaat tccctctgga tagtcgatcc aatcgaaagc agggcgaagg cgatctc
//
LOCUS       DAOQYI010000002       210139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_36_length_210139_cov_72.834204, whole genome shotgun sequence.
ACCESSION   DAOQYI010000002 DAOQYI010000000
VERSION     DAOQYI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 210139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 210139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210139
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36_length_210139_cov_72.834204"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKL93_01055"
     CDS             <1..702
                     /locus_tag="PKL93_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ69885.1"
                     /translation="FASPFSMEDMDLINLIVVDITEALAAKNKLSETLGRLHTALEGA
                     VEFVSKVVKARDPCTAGHQKNVSRLATAIAERLGLDDNRSRTVRIAGLLHDVDKVSIP
                     VEILSKPVRLTDLEWSLIKRHPIVGYEILYDVKLGGPIAEIVKNHHDRINGSGYPEGL
                     IGSELSLESRIVAVADVVEAMVSHRPYRASRGLGEAVQEIKGNSGVLYDEEVVDACLA
                     VIDSGFKIDRSDSRL"
     gene            720..887
                     /locus_tag="PKL93_01060"
     CDS             720..887
                     /locus_tag="PKL93_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69886.1"
                     /translation="MNVFIVILAIFGGLVFLGLAVKFAFFGLRVIFGLAFVGLIVFAI
                     IWLLSKVLLLF"
     gene            1103..1843
                     /locus_tag="PKL93_01065"
     CDS             1103..1843
                     /locus_tag="PKL93_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ69887.1"
                     /translation="MKRGNTYILLIATISLTVFLTLSVLNFLRPYQPSKDAYYSLSSS
                     NEVIYCFKDGTHIFTPAKGSEIGIVFYPGGLVDPVSYAPLLKRIATSGVSCFLVEMPL
                     NLAVLSPNRAKNILKDNPEIRRWILAGHSLGGSMAAKFAYYNQGRVDGLILLASYPGK
                     DTSLSESGIHVLSIYGSNDKVLDIEALESGRALLPEETTYLKLRGGNHAQFGYYGPQR
                     GDGDALITLLEQQEMTVSAILSFIDNAM"
     gene            complement(1858..2298)
                     /locus_tag="PKL93_01070"
     CDS             complement(1858..2298)
                     /locus_tag="PKL93_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ69888.1"
                     /translation="MIRGVFFAIAAGIVIAMQSVFSARLGEKIGFFGSNFFIHGTGFL
                     LASVLLFFFGKGEMSLESLKGLNPLYYTAGFIGVLIILSISQGVSSLGASRGIVIIVV
                     TQIVFALIINVTGLFGELPIDLTPSKVIGLLLMLSGVLVYQLAK"
     gene            complement(2668..3759)
                     /locus_tag="PKL93_01075"
     CDS             complement(2668..3759)
                     /locus_tag="PKL93_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69889.1"
                     /translation="MKRMALLSILVLIVQLAFAFTGSFGGSRTEELSSVVGGSDGILA
                     VGFSEYYDEPYMQTIVIKTDSDGNISWTKEYGTPVDDIGRCIVLTDEGMALLVSRVEM
                     TSGAKLMATLIDGNGSQVWQNFYGKYRDYSPWSVVVTDDGFVIAGQCMTDNNYLQASL
                     IKIDLSGRTVWEREFGKDGIDGAQAVTKLDDGNILATGYSWIEERQYLWLALLDSYGK
                     TRWQKLIGNSEGPFLEGRAAIEAENGFLVGGISSSFSGKAYLAKIDTFGEIVEETLLD
                     DKYPALLSILKDNDNYVGLASFVTVEDEGFGLIEITKEGEVLTHEAFNVDSFEPHAFA
                     RIGAGKFVVVGTKSTDENGRQAYWISADF"
     gene            4158..6533
                     /locus_tag="PKL93_01080"
     CDS             4158..6533
                     /locus_tag="PKL93_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ69890.1"
                     /translation="MLQLKEASKIYRTGELEQTALHKVSICFSNDEFVAILGPSGSGK
                     TTFLNLIGGLDRCDMGDLVINGRSTRDFKDWELDAYRNKSIGFIFQSFNLIPHLSLLD
                     NVEIGMTLGGVPSSEKEKRAIELLEKVGLADQAHKKPCQLSNGQMQRVAIARALANDP
                     DIILADEPTGSLDTQTGEETVKLIKEVARGKLIIMVTHNEEIANRYADRIIKLKDGKI
                     VSDSRPSDDDDPQPELIIKKTSMSFPAALRLSATNILTKKWRTALTILASSIGIVGIF
                     LVLALSSGFGRQLSEFESQTLSTFPIMVSQTTVDLESQQYRQAESEKRFEEDDSDVLG
                     RFVYPYDPRDEILIHNNDLSDEYLAYVESIDPKLLSGFTYTRHINMNLVAKYGEMAKT
                     VDQTSIKFTAYPEDLDPESPGYLENNFDLVAGSFPKEFTDLVMIVGKGNRISNVILQA
                     LGLDHDKERIGFEEIIGLEIKAIYNDDFYLRKGMSFTPKTSLRDLMDLYYGDLGIMLK
                     IVGILRPKAQIEFSVLDEGIAYSDRLAQKFIENAVDSEIVLAQKDLYVNVLSGEQFAS
                     DLFNVLSVIPPDITARLVGGVNLPITKRNILQKLGAYKTPVSVVLYPKDFKSKEEVLK
                     YLDSWNEGKAAENRILYIDLASTITGLLAGILNGSTFVLLSFAAISLVVSLIMIGIIT
                     FISVTERTKEIGILRALGARKKDISTVFNAENFIIGAFSGALGILFASILIVPLNSII
                     ERLTGLSNVAYINPFYVISLMVASILLTVLGGLIPSRMASRKNPVDALRIE"
     gene            complement(6658..7296)
                     /locus_tag="PKL93_01085"
     CDS             complement(6658..7296)
                     /locus_tag="PKL93_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69891.1"
                     /translation="MTKRRRTSPALSIGLFLIMAAIIGFVFSLISINRVGQMKAELSQ
                     MRRQYDEDLKAIEDDFEAKLASVERLLGSGGTLEQYIVAKNFLSNYAIDLETIITEAQ
                     SSSDRPYFRIFVTGTKEVWVGVKKNSADTAYFFQKNLKPGLSQEKFFYFKNPEIETKY
                     TVMVGRDAYVRSGAPENIYLLFFGFGSGKLVKMPSVEITNISEALDLYIPGS"
     gene            complement(7300..8070)
                     /locus_tag="PKL93_01090"
     CDS             complement(7300..8070)
                     /locus_tag="PKL93_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ69892.1"
                     /translation="MRESWEYYSEIASRYDYMYEEPYWNLYHRIVERLLEDHVKTIGR
                     TLDIGTGTGRWALYLAEKAHEVVGVDLSQEMLSVASMKAGLADLKIDFVHSNAERLPF
                     EKECFDYVLAMGDLLSYAKNTSEVLAECQRVLKRGGLLLATVDNAWAFLHDFLSRGEY
                     SMAKRLVEKDKIPIGDSSVSSIVFATKPFFPEEIGHLLNMNGFDLIDISSVVAFYPYD
                     EQALAAKIDSAVDWELKYCRNRETLARSEHLFLCGKKR"
     gene            complement(8488..9261)
                     /locus_tag="PKL93_01095"
     CDS             complement(8488..9261)
                     /locus_tag="PKL93_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69893.1"
                     /translation="MTARDVLTILGAYARSFGESLVDKPTINKSILSSNIKFYKTLKW
                     INILASFLLFVPVFGSIRDFNLPHMVGYLIMAAFGYSTWIFQGITGVPIYEETRFLTL
                     LHLTLHSFFGIWLEFYNNFPLFDDFLHCFGGFWGGFSLFPFIFGSAIAWSNLPPKAVE
                     WKVWLSGISIVNMIGVFWEIGEFISDKIFGRYPGYRMAQESLNDTMLDLIFNNFGATI
                     GILLFWRYLKRSKDINTFVKDMGNKLGEFFVKEKELLRR"
     gene            9426..10211
                     /locus_tag="PKL93_01100"
     CDS             9426..10211
                     /locus_tag="PKL93_01100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ69894.1"
                     /translation="MKPKKSFDFDEFALVYSKYRYPNEKLVEHITNRIRYADRILEVG
                     CGTADYLSILSELLYAHGFGFDTSPSMISEGSKKNQRIELAIADANGPFPYGNSEFDF
                     VFNINLVHYISDLENLFKESFRVLQNDGSVLTVTDSCQDISERTLTSYFPETLDIDRG
                     RYPGNSSIISAMKKAGFEGIYTTKVKTSFEFKKQHFEQYRNKAYSSLRLIPEESFEEG
                     IKRLGSDMERGLVLGKKAYTQIWGIKIAKRDFDLPMIYTTSFH"
     gene            10245..10742
                     /locus_tag="PKL93_01105"
     CDS             10245..10742
                     /locus_tag="PKL93_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69895.1"
                     /translation="MRKLVVVSIITILSVYGFSMAVSVGIGFNLDMSSGALVTPVFIQ
                     FSAGIPYLQGTIHAYVFSFDWSSLVVKGFSLPDHTYLGIQTKITVHKSIYVKGQIFWS
                     LKHITSLIGGERTMAGTPLYTRIGLGSNVSSIGLDAGFDGYWQLEPSSTVPFARPYLL
                     VNFTF"
     gene            10886..11866
                     /gene="thrC"
                     /locus_tag="PKL93_01110"
     CDS             10886..11866
                     /gene="thrC"
                     /locus_tag="PKL93_01110"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNQ69896.1"
                     /translation="MWRYREALPIESDRSIVTMSEGYTPLIEEKICGKNVFIKMDHLL
                     PTGSYKDRGASLLVSYAREHQVSSLVEDSSGNAGCSIAAYSARAGISCEIFVPDHTSV
                     GKLRQIEAYGSKVRLVEGSREDVSRKILEQTKESFYASHVYNPVFLHGTKTLAFEICE
                     QLGWNSPGAIVVPVGNGTLLLGASIGFGELYSAGIVDNLPKIIAVQAENCAPLLRAFE
                     MNQKDSADVNAEATVSEGIAIEKPVRGAQILKAVRDSRGTFVGVSEQEIIETWKEMSK
                     RGHFVEPTSAATIAGLKKYISKTDPNETIASVFTGHGLKTACELFSWNKQ"
     gene            12103..12789
                     /locus_tag="PKL93_01115"
     CDS             12103..12789
                     /locus_tag="PKL93_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNQ69897.1"
                     /translation="MVKLFWDPTFIILIPAIILAAYAQFLVSSRFAHYSKVKSTFGLN
                     GTQLARQLLDNAGLYDIKIERIRGNLTDHYDPKNRIVRLSDTTYNSSSIAALGVVAHE
                     IGHAIQDKEKYVPLIVRNAVVPVAQIGSSLSWIIFIIGLLMVSPVLIRIGILVFSAFV
                     FFTLVTLPVEFNASSRAKKLLSSMGMPSKELEGVSSVLGAAAMTYVASAATAILQLFR
                     MLFLSGAGRD"
     gene            12789..13793
                     /locus_tag="PKL93_01120"
     CDS             12789..13793
                     /locus_tag="PKL93_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNQ69898.1"
                     /translation="MQGARSIALQTLSFFDANGYISFRKLDIALSTLSSQDRSFCMNI
                     IYGCLRKRVSIDFELSRFLTKPSKLPHAVLNALRIGAFQILYMKSIPEYAALKSSVDM
                     IVVKEFKGLVNAVLRKLINGGPAKRKPLNILYSHPEWLVNYWREFAWIDDFEEFLEHN
                     QTPPVQTVLSLGRENELIKNGFIFDKSEYSDLSCVFQKGSSIENLQIIDEIEYLLSKT
                     AIPVLTHKGSLTGKINSMPWLLHTLTPEKIDGYSKVAVESLGNFSREHNEFIYYSQAF
                     TVEENKHALDVLEGFEPVMMEDFFAEHKISARFDGKGYWLQPWKAPAACYLARVRSAN
                     "
     gene            13790..14809
                     /gene="rlmN"
                     /locus_tag="PKL93_01125"
     CDS             13790..14809
                     /gene="rlmN"
                     /locus_tag="PKL93_01125"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNQ69899.1"
                     /translation="MKDILSLDLDELRRGIISFGEKNYRANQIFKWVYVKRVLDFSEM
                     TDLPMSLRNKLGSLFRFTTMKEVERQISIDGTEKFLLKLEDDNHIETVVLKHPRHITF
                     CISSQVGCALNCSFCATGASGFTRDLSASEIVSQVILMENSIGRPVDNIVFMGMGEPF
                     LNEANVYKAIKILHDPVGRNLGIRHFTISTAGIPEGIKRLADSGMDVRLSVSLHSASE
                     ETRSSLMPINKKYPLDSLRKVLDYYQQKTGNRITFEYALIKGVNDSTEDLTKLGEFLT
                     GIKSFVNIIPVNPVKPVFDRPSDKEIAVFSESLKKAGFECAVRHEKGTDIDAACGQLR
                     QKRRE"
     gene            14812..15714
                     /gene="rsgA"
                     /locus_tag="PKL93_01130"
     CDS             14812..15714
                     /gene="rsgA"
                     /locus_tag="PKL93_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNQ69900.1"
                     /translation="MERRKGVVVRFGSRNMEVVDAETGGRLICTMPGRFRLQGIRPIV
                     GDRVEYALSGDGQGRIESILPRKTELLRPRISNIEQILLVLSLKEPAVQNLITDRFLV
                     LAEYAKLPVTIVVNKIDLINNEELDRFSDVYGEHYEICPVSSKNGINLDRLREVLKGK
                     ISVMAGMSGVGKSSLLNSLNPGLKLRVSEISRGLERGRHTTSYVELLQFEFGGLIADT
                     PGFANLELPVIAPENLDKCFPEISQEQGMCAFSDCVHVDEPGCYVKELVEAGSIHRSR
                     YESYLIMYNELKEREQEKGGRKYG"
     gene            15707..16354
                     /gene="rpe"
                     /locus_tag="PKL93_01135"
     CDS             15707..16354
                     /gene="rpe"
                     /locus_tag="PKL93_01135"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNQ69901.1"
                     /translation="MARISPSILAADLTELAREVIRVRDADYLHIDVMDGMFVPNITF
                     GVPIMEALGRLHHPPLDVHLMIEDPSRYVKEYAALGAKNLHVHVEGNYHLHRLLGQIR
                     ETGSRAFVVLNPSTPVSLLDEVLSCADGVLVMTVNPGYTGQEFIPEAAKKIDVLDRIR
                     TERNLKFEIAVDGGVSLDNAQDLVNRGADILIMGAAVFRSENPSVVVRKIKELKR"
     gene            16351..16938
                     /locus_tag="PKL93_01140"
     CDS             16351..16938
                     /locus_tag="PKL93_01140"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNQ69902.1"
                     /translation="MKLYLVTSNENKLKEVRLILPEGFDVESIEAIAPMKDIVEDAGT
                     FFGNSLKKIEAYKDVGVPLLAEDSGLVIESLGGFPGVNSARFMENSHYSEKMQIILDR
                     MVNEENRAARFVCAALFYDPSNSVLVGVEEQVEGIIARKQAGENGFGYDPIFVPQGYS
                     QTFGVLGDSIKKELSHRGKAFRKLFSLVYLYTGKK"
     gene            complement(16892..17779)
                     /gene="lgt"
                     /locus_tag="PKL93_01145"
     CDS             complement(16892..17779)
                     /gene="lgt"
                     /locus_tag="PKL93_01145"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNQ69903.1"
                     /translation="MKKIVVSIVIASASAVALFFFLRAVFSGQLILNPVIVENLGPFS
                     VRWYGVMIATGIIAAYLLGRRQGLKEGIKEDYLIEGVFIGIVFGVLGARIYYVAFNYE
                     IYRGDFWSIFRTWDGGLAIHGAFFAALLVTFLYVTFRKKADLRFLQVTDIFTAVLPLA
                     QAIGRWGNFTNYEAYGSPTDLPWKMFVPLRYRMPGYSEFEYFHPTFLYESLANVVIFA
                     VLYWYLGKRKNYGEVTALYMVLYSIVRFFIEGLRLDSLYIGQSEVRTAQAVSVILLIV
                     GIALFIFSRYKGKQVKRVS"
     gene            complement(17849..19153)
                     /gene="tig"
                     /locus_tag="PKL93_01150"
     CDS             complement(17849..19153)
                     /gene="tig"
                     /locus_tag="PKL93_01150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNQ69904.1"
                     /translation="MEKSFVKKEENVETFLFAFDSEEIRKAENDVARYVNQQYTIPGF
                     RKGKVPLSIVRNFLAESFEEMVLEVLSDKIEEELKDEKILVPAVITEQKMEGEGAVIE
                     VKLHRDPEVKIRDYENLDLKVPKQDEVVLNYVNNRLEELRNEHAIVEPKDGPVENGDL
                     VKIEYTIIKDGKKIAENKVQELSVVPEDDRPIVKNIIGKTKDDVVEFTRTFEDSDNEY
                     FYSVKVLDVLRKSPLDLDDEFAKTVNAEANTLEELKEIVRREGMESFLNWKKDFLRQQ
                     AMDKINDLVEIEISDATLDYLVQRTIENSKKEKSYEKYLKQAGSEEKLIEELRSGVFD
                     EIKKARFIDEIASREGFRAEEEEIQAYAEEMAPYWGISAERAKEMVNTRRDVKEDIVS
                     TIIRNKVLDNVIEKASISDMEPSSDEQLEISESGVEEAPPKE"
     gene            complement(19255..19713)
                     /locus_tag="PKL93_01155"
     CDS             complement(19255..19713)
                     /locus_tag="PKL93_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNQ69905.1"
                     /translation="MSDSHDNMPRISESVEIAVDRDVEVLIHCGDLVSPFAAKALTRF
                     KGELHVALGNNDGEIVGLKNILGSSLIKGPAEIKIHGRSVVLMHEPFGLSGAVITDFL
                     FYGHTHHLDIRLNSRPVIINPGESCGYLTGKPTVVVIDPDNLEYELIQLR"
     gene            19846..20508
                     /locus_tag="PKL93_01160"
     CDS             19846..20508
                     /locus_tag="PKL93_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNQ69906.1"
                     /translation="MNNNKLVVRFCCGGFLRFLSHQESATAIERTIKRSGLPVAYTQG
                     FHPRIKISFSPALPTGVMSLANYVLLETDKLSQNVVNLLNSVSNFTIRALKAWYLPRD
                     YAKIDDLLDSYRTALILPKRSYYPERFDASAIVTKKTKSGYKKYNAGEVFMDLSVTSL
                     RTVHVVKYSQPIESMVTAEEILKILSKEGTASYEGVIAFVEDGMVNGRSTSDILDEIG
                     GI"
     gene            20510..21268
                     /locus_tag="PKL93_01165"
     CDS             20510..21268
                     /locus_tag="PKL93_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ69907.1"
                     /translation="MSGHNKWANIKHRKGAQDAKRSKVFTKIIRELMVSAREGGSDPN
                     TNTSLRSAIEKARSANMPKDTMEKAIKKGAGELEGQSFSEALYEGYAPGGVALLIRCL
                     TDNKNRTAQEIRHTLSKYGGSMAESGAVSWMFERKGVITVAKEQISDLEEFQLLAIEA
                     GAEDIKDEEDPIIIVTSPESVSDVKTALEENGYTLSYEMSYIPSNSLKVEGSDAAKLL
                     KLLDILEDNDDVQEVYDNSDIDETEMEALAEQMG"
     gene            21310..21786
                     /gene="lspA"
                     /locus_tag="PKL93_01170"
     CDS             21310..21786
                     /gene="lspA"
                     /locus_tag="PKL93_01170"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNQ69908.1"
                     /translation="MLSLLGITLALIMDQVSKYFVENGMTYFQRIDLIGELFGLRYVR
                     NTGVAFGLFKNQEPWLLAFVAIGIAAMIVIVSNAHSSKLSGWESFLIGLIVGGALGNN
                     LIDRLRLGHVVDFFELKGFPAIFNFADLCIVFGAALLTLSVYRREKRASRDNSLQP"
     gene            21755..22687
                     /locus_tag="PKL93_01175"
     CDS             21755..22687
                     /locus_tag="PKL93_01175"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNQ69909.1"
                     /translation="MLQEIIVSSREDGWRLDKVVVEKAPLWVSRTFVQRQIKNSKVFV
                     NSRPRKPSYKVKIGESITFDLPEKPKVASVEPEAIPLKIVFEDRDIIVVNKDPGIIVH
                     PLPRKQTGTLVNALLYHCKDLQGIGGVTRPGIVHRLDKDTSGIIIVAKNDLAHVSLSS
                     QFKSRLTTKEYIALVRGKTPPFGKIDYSIARHPVNRLKMSVNENGKESLTYFQTLSNF
                     SSIASLIIVRPRTGRTHQIRVHMKEKGFPLLGDAVYGKAREDEIFGIKRQMLHAMKLT
                     VSHPRSGIRMTFIARLPEDMKEAIVNLSELEAKR"
     gene            22684..25380
                     /locus_tag="PKL93_01180"
     CDS             22684..25380
                     /locus_tag="PKL93_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNQ69910.1"
                     /translation="MKTAFVITKYELHASVLEEISTTAELKKRGYERCVILDSSLSSY
                     AKWHLSLSRSGIVAVPGIRTGNDYWIARNERGFEELLRKETNDSENLIHISGKPRNLD
                     SSSGNPIWECRYLDHESEKFRVYASLQPEIEVADYSLPNDKNYRELEHDLLHLLSELP
                     RNFSLKRIEHVFPVRVSAESFSSLLCKALEEKKMGDEYLSRLKRELSVILAKNIQDYF
                     MTVSKIVDIAKSIGCWIGPGRGSAVGSLVSYLLEITRIDPVAEDLFFERFLSLKRADP
                     PDIDLDVDDKSRPVLLKKMYEYFGNDRFCLIRTYSTYGFKGAARELGKKLGISKDNIS
                     KLIEWSKDGKRFPYAFEEDLDMKRLFDMSKLIAGLFSGYSLHAAGVILSGISLKGLIP
                     IDFSEDLPVSLWDMDSLRIVGLQKIDILGLRNLSLLKEMTQGKEPWESPACDSNTYET
                     LGSGYTTGIFQLEAPYATKIIRSVKPSSLRDLSICIALNRPGPIKSGVTERFLKLSRV
                     PSEIEKMRKRVPVLAETAGLLVYQEQVLKIASSLLSLEADDGELLRRALSKKDRDAVD
                     RLFKSSPGYSRLPDERRAKLYSFLLDFAGYAFNKSHSLSYAVISFWLAYFKSNHPEIF
                     YPLMLRELPRTSFGRLVAEMRDRGLKLSIENEVEGRKIVSLSIPQLLKKGELKPKPFE
                     DSFFTFVRNNRSKYQARDLERLIKSGYLDCYGSRNDLLKKMNDALTGVNPELKSIRSV
                     FGYKEELQEAREEDTPVERAMMEIETLGFNVTEIQRPDISSEVTDFEITSAVASLRTG
                     ISPYKRIDYLGKSFVTDGRTFIQIESHVPLQGFVLFNNGNPIEMKERILEVNRIFHGQ
                     IDRSFLAVGSKRENVVVKVRGTEKIIPGARPMGVDADKIIWR"
     gene            25395..26006
                     /locus_tag="PKL93_01185"
     CDS             25395..26006
                     /locus_tag="PKL93_01185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNQ69911.1"
                     /translation="MYIYFVRHGETEWNNSNRWQGRSDIPLSEKGRKQAEITGTFLKE
                     HVPSVAAIYSSDLKRAKETAEIIAVSYAETPVANPVLREADVGLWNGLEISEALKCYG
                     NLIEKWRKDPWADIPGTEPLGEVQRRAASFIRYLSGNFQGKHVIVVSHALLIRTAICY
                     AAGLPLENHYRLSLHNCSISTIKIEGAEMRLLELNLWRHMEEN"
     gene            26010..26705
                     /locus_tag="PKL93_01190"
     CDS             26010..26705
                     /locus_tag="PKL93_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNQ69912.1"
                     /translation="MLLRVNLLPRTIDESTDLAVVIDVLRASSTITTALQLGAEKVIP
                     VSNVKQALEERNRNPEVVLVGEREALKIPGFDLGNSPVELSESFVKGKEVVMTTTNGT
                     VAALKSRKAKRITVASFLNLESIARHIEREKGRVEIQCAGSDGEPSLEDTLLAGALVK
                     RLGDVGINDSCWIASFLYESVRIDLKDFIAENGVHAKKLLSLGFSEDLSFCAKINLYD
                     LVPLWRNNGFVRG"
     gene            26710..26922
                     /locus_tag="PKL93_01195"
     CDS             26710..26922
                     /locus_tag="PKL93_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69913.1"
                     /translation="MEFVSNAFFILAMGALFLSLIFFEIGTKKVRKPKSEVKPEDYKP
                     YDRKGWYSLLAAGGFLGLSLLFALIL"
     gene            26942..27583
                     /locus_tag="PKL93_01200"
     CDS             26942..27583
                     /locus_tag="PKL93_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69914.1"
                     /translation="MPNKLAVDNVVSKLPGVHSARIVYDESGEMVEIHVLADITKPAK
                     QLVRDIETAIYASTGIKIDRKIVSIAQISLDSNDEDEASVVRPYQKEELQGYNFQLTS
                     LETRATTRRLDITVVLDYDGKELEGSSIVDVGDDEKYMAIVESTVAAIREVIPSFRIE
                     FIEKLEYGMTEIVMAVGSSLVNGKRKREAGARLCKKDSLNDFALVVLEIINKI"
     gene            27624..28781
                     /gene="aspC"
                     /locus_tag="PKL93_01205"
     CDS             27624..28781
                     /gene="aspC"
                     /locus_tag="PKL93_01205"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004069 - L-aspartate:2-oxoglutarate
                     aminotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HNQ69915.1"
                     /translation="MDLSHFVSSVTPSATLEFNKKALELSKAGENVVKFTAGEPDFPT
                     PRPIVEAAITALNEGKTKYTNASGIDELRKAIATKMLKENGLSYTPDEIVVSNGGKQA
                     IYNVLKALLNVDDEVIIISPAWVSYEAQILLCGGRPVVVPSELDKGFVPEISKIAEVI
                     SARTRAIIINSPNNPTGAIYPEHFLRDLAVLSKESDLLVISDEVYEKLVYDAAHFSIA
                     SIDGMKERTAVINAFSKTYAMTGWRIGYSATSLRLAKAISKIQSHLTSNVNTITQYAA
                     LKAFEVDTSSMVEEFRARRDFVFSKLKKIGLRFHKPAGAFYYFIDIRPFLGGRFKNSN
                     DFAIALLEEEKVGMVPGSAFSYEGFIRMSFSSSLEELDEGLNRLENYLRKL"
     gene            complement(28782..29912)
                     /locus_tag="PKL93_01210"
     CDS             complement(28782..29912)
                     /locus_tag="PKL93_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ69916.1"
                     /translation="MFQGYVKLLELATNLFTMYRWNNTPTLLRTNEAENAFISAQYCL
                     LMSEMAILKGFEIDQKELFQRLVLKELPKCLLSDISVDTKILIKSLSPDKWNDVFEKT
                     VEEIIQYLPDERHDAFFFSMVHSKDNSVEGKIIQTGDLLSAKLEAGLHARSFPEFFER
                     PLKDLEARIKDFEEFGPYRLITGNNWLSRYTNALMVLLRAIRWNRLNRNVPTTVAAHS
                     FYVTIIAYILSCIEEEQGNSVNPVESVKRALLHDIPESMTGDIITPTKKKVPGFEEVI
                     AQVEEQMVTENLLDGMPDELIKELKSRMLNPFESAEGKIVWAADQFAATVECLMEIRS
                     GNTQYAFRDAMNRMLDDLSRSDLQSVQFLTKSFRWSLDWTGR"
     gene            30011..30625
                     /locus_tag="PKL93_01215"
     CDS             30011..30625
                     /locus_tag="PKL93_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNQ69917.1"
                     /translation="MNIEKWINRTYPVFEESSTLEDVFSVKALAGLTNIISVDKEGRL
                     SGTARLDAIDDFDLSRKLSEIVVEPVFYCRDSDFIEDAALMLIESHDYVLPVVNDDFV
                     VVGAIGVFEILEGLMEFTAMDRPGARISIILEDKPGALRDVVDFLAEREVNILSIITS
                     TAESKEFKRVVIRTDESDISLIADILGEKGISFESISEEEGFSV"
     gene            30618..31406
                     /locus_tag="PKL93_01220"
     CDS             30618..31406
                     /locus_tag="PKL93_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNQ69918.1"
                     /translation="MSETLSYLILGAVQGATEFLPVSSSGHLTIFSSFLKVPLDSESK
                     AAFFAVLHLATFFAVLLFTFKDIWGILTGLSKPEKRKISFRFIVLLLVATIPAVLVGF
                     LLEDEIKSLFSDVAFASIMLFAAAAALFISDSLKGERKILDLSIAGALSIGIFQAAAI
                     APGLSRSGLTIFGALLIGMTRSDAVRFSFLLSLPVTFGAGILELSKVSLPISTVLLSC
                     LLAFSTGIVGLWLLKKLVLKRRLRFFGFYCIIIGIVGLIFRGVM"
     gene            31408..32028
                     /locus_tag="PKL93_01225"
     CDS             31408..32028
                     /locus_tag="PKL93_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ69919.1"
                     /translation="MDLNLLFPGSLVYVPGMMDASTSSCVLLVEDYKKILIDPGGFAS
                     MKVLEDGLTSLGLSPDDITDILLTHFHLDHAFNSVFFSKATIHLHESFSTRDYSSFGP
                     IIGSVYSKIIDSWKCIETFSGGERILGAIDVLSSPYHSRDHVSFFLQTENHGRVFICG
                     DVCARQIHYHEMRKGMRSDPAAAFVLKHFERADAVIFSHDLPLFKR"
     gene            32033..33439
                     /locus_tag="PKL93_01230"
     CDS             32033..33439
                     /locus_tag="PKL93_01230"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HNQ69920.1"
                     /translation="MKVLFVSYEVYPIAKVGGLADVAGSLPKYLKELGVEIDLLMPFH
                     KSIDKNMAIKTDENVSTRFVKREVSFEVYRAKLPKSDVNVFLLYNERLMDSKEIYGAS
                     DLGFQAMSFSDAAAGFAVDKGYDIVHLNDWQTALIAVYLKQCEAECKTLLSIHNLAYQ
                     GVYGKEYLDLSGIEKSNWDEISEAGKINFLKAGLVFSDKLSTVSPTYAKEIQTKEYGA
                     GLEKILSRRRSDLVGILNGIDYYEYDPERDERLWANFSQDNIEGKKFNKSNLQQLVGL
                     PKRDDALIGLISRLVDQKGLDLIEDIKDELMKLPIQIVVLGTGESKYEDMFRILQKNY
                     PEKVAARLTFDLDLAQKIYGGADMFLMPSRYEPCGLGQMFAMRYGTVPIVRYTGGLAD
                     TVHEFDPENNGNGFGFVEYDAKKLLGALKKALKVYFQPELWKKVVRNAMRQDFSWRVS
                     AMNYLSLYKKILEVNNDA"
     gene            33432..34439
                     /gene="galT"
                     /locus_tag="PKL93_01235"
     CDS             33432..34439
                     /gene="galT"
                     /locus_tag="PKL93_01235"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNQ69921.1"
                     /translation="MPELRKDPIIKRWVIIATERARRPHDFINAKENVESAFCPFDYG
                     NEHTTPPEIMAFRPADTVKDSPGWWVRVVQNKFPALDSNIEPERFGHGMYDVIKGFGT
                     HEVIIETPDHNASMATLSYGQVREVVWAYKERLQTLEKDNRIKYILIFKNHGREAGAS
                     LVHSHSQLIATPIVPKRVAEEIAGAAEYYRFRERCVYCDMVNQETLEGTRVVEENSDF
                     VAFEPFAARFPFETWIVPKVHSHNFGEISATQIESFSIILKNTLLRIYKALDNPPYNF
                     MLHTGIRGIDESNHYHWHLEIVPRLTRVAGFEWGSGFYINPMPPEHAAMYLREVSVEE
                     E"
     gene            34442..34741
                     /gene="gatC"
                     /locus_tag="PKL93_01240"
     CDS             34442..34741
                     /gene="gatC"
                     /locus_tag="PKL93_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNQ69922.1"
                     /translation="METKVSDELLKHLEGLAKLELLEDERIELKKDIEEILQYMTLLD
                     GVDPDAEEMVTPVEKSLEPREDELVEFKESRLIRDLFPDTEGSYLKVPRIYKEEK"
     gene            34745..35083
                     /locus_tag="PKL93_01245"
     CDS             34745..35083
                     /locus_tag="PKL93_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNQ69923.1"
                     /translation="MSKEAGKRYEDLACEYLKENGYRIIARNVAYRFGEIDIVALEGR
                     ILVFVEVKGGSSLVLPRYRVDERKIRRLELAAQRYILTEEPKFEEARLDVIEILESGA
                     VNHFRSVGRW"
     gene            35085..36734
                     /locus_tag="PKL93_01250"
     CDS             35085..36734
                     /locus_tag="PKL93_01250"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNQ69924.1"
                     /translation="MLRGERAVNQEYKRWLDNASDEIRDELKDLTPEEVRERFSLDLE
                     FGTGGMRGVMGAGTNRINSYTIKRASLGFGRWISENYSDPSVVIAFDTRHKSAQFAQT
                     TAEVLSSMGIRVHVFIEPMPVPILSYAVRELKASGGVVITASHNPPQYNGFKVYTSDG
                     TQAVPGYAEEIIKRVEASDYFEDFSPRSELINSIPNSLLEKYIDTVKENILGLTSDYE
                     ELNVVYTPLHGSGNFPVYKALTVLGHRVKRVEEQAIPDGDFPTVQSPNPEDPRAFSMA
                     LDVAREIGADLVIATDPDCDRLGIMVAHNGDYAPLNGNQTGAIMTSFILERMKDSLPS
                     DPFIVKTIVSTDLVKRIASKYGVEVKETLTGFKFIGEIIEKAEMDGKGNYLFGFEESY
                     GSLFGKHARDKDAVSAAALACAIGGYLKRKEMTLIDYLEEIYSEFGYFQEALVNKEYE
                     GIQGKEKIESIMTGLRHIEPSKLKEMAIREMRDYLNGYGNLPPSDVISMEMENDCKII
                     VRPSGTEPKIKFYLMARGENREEAEKKIEEMKELVERIVCL"
     gene            36911..37339
                     /locus_tag="PKL93_01255"
     CDS             36911..37339
                     /locus_tag="PKL93_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69925.1"
                     /translation="MRRKITFERKHSDSNLLYFKYELEVPEALEGYFDQTTFEVISKN
                     LSIRETTEEVYKMLKDYEEGSLSFNDQRFSSWLAMKTGELQLSVNEKKELEKSLIKYV
                     ETIVGRILWTVYNGDLPKMGKDLSSIVYLYTEMLDLIALK"
     gene            37336..38094
                     /gene="truA"
                     /locus_tag="PKL93_01260"
     CDS             37336..38094
                     /gene="truA"
                     /locus_tag="PKL93_01260"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNQ69926.1"
                     /translation="MIMKRFAAIVSYDGTDFFGFQNQPGLRTVQGKFEEALHRIHKFC
                     ISSQGAGRTDTGVHGFGQVIAFDSNLDRLEATTMRDALNANLPSDMYVRKVHEVDENF
                     SPRFAAKKRIYHYYIQTSSEPDIFRRRFVWWFPYSLDIKSMRRAAVNLLGEHDFAAFR
                     TGKDDRNPVRTITAIRIIRYKPYIILIRVEGISFLKRMVRNIVGTLVKVGTGSVSEES
                     VSEFLSSHDRSTLPGTAPPQGLVFYKVIFDEFET"
     gene            38846..40297
                     /locus_tag="PKL93_01265"
     CDS             38846..40297
                     /locus_tag="PKL93_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69927.1"
                     /translation="MKSLLVGLLLILSFSGIMAQEIFDFVPEDVGYFLILRGLRSTME
                     ALRDNTNFFGAYLGDNGYGAERAFYGIIDAAGYNAEVDTSFLKSALNNDILLAGEGID
                     LSLDDLLMFDPFYLLEKLKLTENRVFMAWRTDSSFELLKAVAALLDMSIFVQPGESVN
                     ELRSNAGTLFYNAGDDYLIIGGNKDAVTLALKTYEGEGKRLLESNGKGREVAEMSYDY
                     ALAGYIDGDRFHLDLGLESTFSIESTILFARPEGHTLTATIVQDTVFLSSEDIQKMLS
                     VTDTKELEASKETFGDYTVFFPSSSVETLRKELAHWFELDLEPYRELADFVVLLSKES
                     IGNARIYGDLVSETPRVTVVFTNKSSDTEKFENSLKEWGALQSAHKDSRIYELENDFD
                     TIYFIFSNETTVLTSLSPDEYYRLLSDSESLSDNISYEFVRNLGLEKDIAHAFIDMKK
                     VFKSLMGVSVDSALFYQQTFDENGNMIHALRFY"
     gene            40307..42760
                     /gene="secA"
                     /locus_tag="PKL93_01270"
     CDS             40307..42760
                     /gene="secA"
                     /locus_tag="PKL93_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNQ69928.1"
                     /translation="MGLLSRIFDKNNMLLKKYSKVVESINSLEKTVSRYEEVDFQKKT
                     EEFKARVSEGETLDSFLPEAFALAREAAKRTVGMRPFDVQLMGALALDEGKIAEMKTG
                     EGKTLVATMPLYLNALSGRGCHLATVNDYLAKRDAGWMGPVYEYLGLKAGFIQAAMEP
                     SQRKEAYGCDITYGTANEFGFDYLRDNLVYSLENKVQRGHNYVIVDEADSILIDEART
                     PLIISGPAQDSSNLYRQFAFFAKRFVAEKDFVVDEKDRTVTLTEEGIAKAEKLLQIDN
                     LYDPSNYDYIFHLLNALKAMNLYKREVDYLVTQEGEVVIVDEFTGRLLPGRRYSEGLH
                     QAIEAKENVQIRQESVTFATITFQNYFKLYSKVSGMTGTAATEESEFISMYNTPVAII
                     PTNKKVIREDKEDSIYKTREEKNEAIINEIAERYEKGQPVLVGTTSIEKSELLSKLLQ
                     KKGVPHEVLNAKYHEREAEIVAKAGERKTVTIATNMAGRGTDIKLGEGVVDLGGLYVL
                     GTERHESRRIDNQLVGRSGRQGDPGESKFFLSTEDDLLRLFGGERMQSIMNTLKIEKG
                     QPIEHPLLSRIISSAQKKIEGMHFEIRKRLYELDSVIDQQRSAIYAHRNWVLKGEEID
                     SNVLEIIEDVVERRLSGLDRLPSFDEIKVSFGFLPAGDIESLKNLKSVEDLRSKTLSV
                     LHDIYEEKKSAFGDEFPQVIKYIMLRMIDERWRRHLEAIEHLKDSVGLRAYGQKDPVL
                     EFKKESFILFQQLTDSLYDDIASAIVRIVRVDSDKAKQNADKEFKSLQAVHSDFSGGD
                     KKKEVAGKKKGTKRFKVKR"
     gene            42775..43887
                     /gene="prfB"
                     /locus_tag="PKL93_01275"
     CDS             42775..43887
                     /gene="prfB"
                     /locus_tag="PKL93_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNQ69929.1"
                     /translation="MISYETNSRIQELKEKFESIRATLDLERIDEKLKEIEKRMSDPS
                     IWNDKREASNLGREAQSYRSTIELLRDVEEEFESIEIAVELSGEDESYVKHVEELLRS
                     AEKKVREFELTILLSGEYDINNCFISIHPGAGGTESQDWASMLMRMYMRWAEANRYKI
                     TIVDELPGEEAGIKSVTINLAGPHAYGKMKFEAGVHRLVRISPFDANHRRHTSFASVS
                     VFPEMDEVPEIEIRPEDLKIDTYRSGGAGGQHVNKTDSAVRITHLPTGIVVACQTERS
                     QHQNKANAMKMLYAKLFEIEIEKKRSEKLKLMGDQKEISWGNQIRSYVFQPYTLVKDH
                     RTDFETGDIQSVMNGEIDEFIEKELLFFSSIEKSLE"
     gene            43954..44853
                     /gene="rsmH"
                     /locus_tag="PKL93_01280"
     CDS             43954..44853
                     /gene="rsmH"
                     /locus_tag="PKL93_01280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNQ69930.1"
                     /translation="MSRQYDERHRSVMIHEALHYLLGGRLTGTYVDCTAGEGGHIGAV
                     LEATKGRAKIIGLDVDSEVLDIAEQNLKDYRESVELFNLSYVDFEIALKQAGVEKVDG
                     FLLDVGVSTFQLKAKGRGFSFELDEPLDMRMNLSQKRTAADVVNSYKEDELSKIIFEF
                     GDEKRFARKIARSIVSRRPLFTTKDLHDAIKAAIPPAERYKRRRHFATKTFQAVRIEV
                     NQELENIESTLRKIPGFLNQGGRIVIISFHSLEDGIAKRVFREKSESELKILTKKPVQ
                     PSKEEVELNLRARSARLRAAERI"
     gene            45249..47027
                     /locus_tag="PKL93_01285"
     CDS             45249..47027
                     /locus_tag="PKL93_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ69931.1"
                     /translation="MRAAYVSLFTEPDLPLVLGVNKIPALRGSIYDSKGRLLASDSLI
                     YEAWLDLGYLRLLTSSDQIDRVLKNVELSFGIPFERLVESLQSNRNFLLLGTAATNDE
                     MQRRITPITKRYISLEMQRERLSFSEYGLERIIGKLDGGGIPLNGIELYYDEELSGKR
                     DGLIRRTLTSSRREEPTNGSDIYLSIDIDIQKMVYDELLETVEKNMADGGLALLVESK
                     TGKILAYAGTYDWDVGLMGIFEPGSTIKPLIYSLALQSGSITETMTFNCDGSIQPVPG
                     LDIIIRDTEGERHGLQTFREAIINSCNVATVQVGGRILNTLGKEEMYRQLTRIGFGKL
                     SGVDLPGETPGILPEAKDWSLISPYQFPIGQGLGVNIFQMVKALNVFPAGGQFITPTF
                     VSAIRNEEGLINIPKKVEGDLFSPKLVSTMIPILEGVVIEGTGTMAKVDGVRIAGKTG
                     TAQKAGPGGYNEESYYSLFYGFFPVEDPTYTLYIMIDTPKAGAYYGGFVAAPMFASVV
                     RNIYGIGVEKEVYEGVYSWKAPDLSGYSLLDLKEIAAIYGLDNIIIHGSGSVVSQIPE
                     AGRPLGDQLEVWLGELNYDIRDVGGQ"
     gene            46999..47940
                     /locus_tag="PKL93_01290"
     CDS             46999..47940
                     /locus_tag="PKL93_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNQ69932.1"
                     /translation="MIYEMLGDSEVLKDFFILERAEGCLTISEDMPDKVNRFRMVVST
                     SGMFGRKSVRLSGFDSWKKEEKTQIEKLLPLFQDEIDVFLDGKVSPKVKSEKKFFDLP
                     KPWEEEKWNDHIITISERAGMKISRAAAESLFRKVGRREYRILRELEKLSVFSNEIDQ
                     LTVERLIDFDKEVEVESLAFDFLNHKEDFLSSSRKCMIPFAYFASVLLNIIIDLGTII
                     DIKKGKMNISWSEIRKLSNTTSIGTSRIARLVGYSFSSTKEQRNDLTRLYSAESLKSL
                     IDLLQEIDESIKNGKIDSEIALFKLHEESSKLQVTLE"
     gene            48050..48424
                     /gene="rpsL"
                     /locus_tag="PKL93_01295"
     CDS             48050..48424
                     /gene="rpsL"
                     /locus_tag="PKL93_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNQ69933.1"
                     /translation="MPTINQLIRHGRKQLKQKSASPALENNPQKRGVCVRVSTMTPKK
                     PNSALRKIARVRLSNGTEVTAYIPGEGHNLQEHSVVLVRGGRVKDLPGVRYKIVRGTL
                     DAEGVANRKQSRSRYGAKRPKK"
     gene            48439..48906
                     /gene="rpsG"
                     /locus_tag="PKL93_01300"
     CDS             48439..48906
                     /gene="rpsG"
                     /locus_tag="PKL93_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNQ69934.1"
                     /translation="MRRRQSEKREVPLDPIYNDAVVTRLINKIMIGGKKSKAEATVYG
                     ALEVLSDRTKQPPMEAFKKALSNVKPLLEVRPRRVGGATYQIPFEVPERRAVALAIRW
                     IVTSARAKSGKPLKEKLALELVDAYNGQGNAVKKREDVHKMAEAGKAYAHFRW"
     gene            48932..51004
                     /gene="fusA"
                     /locus_tag="PKL93_01305"
     CDS             48932..51004
                     /gene="fusA"
                     /locus_tag="PKL93_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ69935.1"
                     /translation="MKERATTLEKTRNIGIMAHIDAGKTTTTERILFYTGKNYKLGSV
                     DEGTATMDWMDQEKERGITITSAATTAFWKDHRINIIDTPGHVDFTVEVERSLRVLDG
                     AVAVFDAQAGVEPQSETVWRQADKYHVPRIAFMNKMDKIGADFLGAIGTMVTKLKANP
                     VAIQLPIGSESGFEGIIDLVQMKALRWTNQEGTEFTYSNIPEFLQAQVEEAREDLITH
                     VAEFDEELMDLYIEGEELPVDRLKAAIRKATLLGQITPVLCGSAFRNKGIQPLLDAIV
                     DYLPSPKDLPPVVGEIIDSERKIEVYPDENGPFVAMAFKIMIDPFVGKLTFLRVYSGS
                     LEKGAYVINTNKNLKERISRLLFMHADKREEVDYIRAGDIVAVVGLKNTMTGETIALS
                     EEKIVLEKIEFPEPVISIAIEPQTKDDASKLTKALVALVEEDPSLKSYVDAETGETIL
                     SGMGELHLEVIVERIKREFNVGLRVGNPQVAYRETIRSKAIGESKYVRQTGGKGQYGH
                     VILSVEPITDKSANFVFEDKTVGGTIPREFIKPIERGVREAMDSGYLAGYPMVNVKVS
                     LLDGSYHEVDSSEIAFSIAGSMAFKEAARKASPALLEPIMAVEINTPEEYMGDLIADL
                     NSRRAKIEGFETRAGLKIIKAHVPLSELFGYATVCRSLSQGRAIHVIQFSHYSEVPER
                     IAEKILGK"
     gene            51041..52237
                     /gene="tuf"
                     /locus_tag="PKL93_01310"
     CDS             51041..52237
                     /gene="tuf"
                     /locus_tag="PKL93_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNQ69936.1"
                     /translation="MAKEKFERSKPHLNIGTIGHIDHGKTTLTAAITKSLAFKGLADF
                     SPFDSIDKAPEEKARGITINVSHIEYQTEKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVAATDGVMPQTREHVLLARQVNVPAMVVYINKVDAVDDEELVELVEEEVRELLSS
                     YEFPGDELPVIKGSALKALEAESPNEWTESIYELLKACDDYFPEPVRETDKPFLMPIE
                     DIFTITGRGTVVTGRIERGAVHVGDEVEIIGLSYETKKTVCTGVEMFRKLLDEGQAGD
                     NIGALLRGVAKEEVKRGQVLAKPGSITPHKKFTANVYVLKKEEGGRHSPFTKGYRPQF
                     FIKTADVTGEIADLPEGVEMVIPGDNVEMTIQLIYPVAIEKGMRFAIREGGRTVGAGV
                     VSSIIE"
     gene            52307..52615
                     /gene="rpsJ"
                     /locus_tag="PKL93_01315"
     CDS             52307..52615
                     /gene="rpsJ"
                     /locus_tag="PKL93_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNQ69937.1"
                     /translation="MAKQKIRIRLKAYDHKLLDLSAKKIVEAVKLTNAKVSGPVPLPT
                     ERTLYTVLTSPHKFKDAREQFEKLVHKRLIEILDPTPKTIDSLMKVDLPAGVDVEIKL
                     "
     gene            52638..53270
                     /gene="rplC"
                     /locus_tag="PKL93_01320"
     CDS             52638..53270
                     /gene="rplC"
                     /locus_tag="PKL93_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNQ69938.1"
                     /translation="MKGILGRKLGMTTIYKDGKAFGVTVVKAGPCTVVQKKTSDGGEY
                     DAIQVGFEELTPERAKKVLTKPLVKKFEAAKVKPHRILKELKVGNINDFNVGDIIEAR
                     VFSEGEKVDVTGFSKGRGFSGAMKRWNFRGGEASHGSKFHRELGSVGNHTEPAKIWKG
                     KKMPGQYGNEKKTVKNLTVVKVDAENGLLAIYGAVPGARGGLLVIKSANR"
     gene            53302..53985
                     /gene="rplD"
                     /locus_tag="PKL93_01325"
     CDS             53302..53985
                     /gene="rplD"
                     /locus_tag="PKL93_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNQ69939.1"
                     /translation="MAQADVVNIAGQKVGTVELSESVFNVEPNEDLMFRYVNMQLSGR
                     RAGLASAKTRSEVRGGGRKPWAQKHTGRARVGSTRSPLWRHGGVIHGPKPKDWSMKLT
                     KKMKKVALRSALSLRLKEGNLIVLDDLKFDRPKTKQLREVLNNLGLDKTQKTLFVLPW
                     QKDEYQNVRLSGKNIYGVKVIIADNPGNKAVNSKENIDGLNVYDIVNHEKLVITADLV
                     RKIEEVLGR"
     gene            53982..54281
                     /gene="rplW"
                     /locus_tag="PKL93_01330"
     CDS             53982..54281
                     /gene="rplW"
                     /locus_tag="PKL93_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNQ69940.1"
                     /translation="MIEKKYYGDVLLRPLITEKTVASGELNKYIFEVHRDANKQTVKD
                     AVEKLFNVKVEQVNIMNMKGKPKKRGVFVGKSRSWKKAIVTLVTGYRIKELEGQH"
     gene            54298..55125
                     /gene="rplB"
                     /locus_tag="PKL93_01335"
     CDS             54298..55125
                     /gene="rplB"
                     /locus_tag="PKL93_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNQ69941.1"
                     /translation="MALRKFNPVTKSRRFMLLPDFKEVTKSEPEKSLLEPIKRNAGRN
                     HHGRITVRHQGGGTKRRYRIIDFKREKMGIPAKVISIEYDPNRTARIALLQYVDGEKR
                     YMLAPKGLKVGDSVQNGDMAEISVGNSMSLERIPVGTIVHNIEFLPGKGGQIARAAGT
                     YAQLMAKEGRYALLRMPSGELRRVVVKCMATIGMVGNEEHSNEIHGKAGRKRWLGVRP
                     SVRGMTMNPVDHPMGGGEGRSKGHLPQSPWGQPARGYKTRKNKKPSDSLIVKRRNQN"
     gene            55145..55429
                     /gene="rpsS"
                     /locus_tag="PKL93_01340"
     CDS             55145..55429
                     /gene="rpsS"
                     /locus_tag="PKL93_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNQ69942.1"
                     /translation="MSRSKKKGPYVHPSLLKKIRELNEKGEKKPIKTWSRASMILPEM
                     IGHTIAVYNGMKHIPVYISENMIGHRLGEFSPTRRFGGHADKKSKKGEVR"
     gene            55444..55878
                     /gene="rplV"
                     /locus_tag="PKL93_01345"
     CDS             55444..55878
                     /gene="rplV"
                     /locus_tag="PKL93_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNQ69943.1"
                     /translation="MTQSVQRGKRSVFHRKRKEEKAATPVTQAKAVAKFVRISPRKAR
                     SVVNAIRNKEVGEAFQILEFSPKKASRLVYKVLRSAVANAENNFGLNVDSLYVEKAVV
                     DDGPRMKRLWPRGRGRADIQQKRFSHITVVVANREEISNPQE"
     gene            55889..56518
                     /gene="rpsC"
                     /locus_tag="PKL93_01350"
     CDS             55889..56518
                     /gene="rpsC"
                     /locus_tag="PKL93_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNQ69944.1"
                     /translation="MGQKVHPYGFRLGVSKDWKARWINEKNYKEYLLEDLKIRDFLKK
                     NYMAAGVSDIYIERPEPGKVVITIKCARPGVMIGKKGSEVKLLRQRLEKLITRQFQLN
                     IEEVKTPETDAILVAEDIASRIEKRASYKRAMKRAIFTAMRKGARGIKIMVSGRLNGA
                     DIARTEWYLEGRLPLQTLRADLDYGYTTAFTKMGIIGVKVWIYKGDVQV"
     gene            56543..56968
                     /gene="rplP"
                     /locus_tag="PKL93_01355"
     CDS             56543..56968
                     /gene="rplP"
                     /locus_tag="PKL93_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNQ69945.1"
                     /translation="MLMPKRVKYRKQQRGKMNGKAKGGTIVHFGEWGIKALEPHWITA
                     QQIESCRIAITRTLKRNGNLWIRIFPDKPITSKGIGVRMGKGKGDVEGWVAVVKPGKI
                     MFEIGGVPDQLAKEALEKAASKLPIRTKIVPRYQIGGEL"
     gene            56968..57168
                     /gene="rpmC"
                     /locus_tag="PKL93_01360"
     CDS             56968..57168
                     /gene="rpmC"
                     /locus_tag="PKL93_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNQ69946.1"
                     /translation="MKAVELQKFTDEELTQMLEDSKRKLMDLRFQLEMNRLKNHSQIT
                     SVKRDIARIKTILRGRELGIRR"
     gene            57175..57417
                     /gene="rpsQ"
                     /locus_tag="PKL93_01365"
                     /pseudo
     CDS             57175..57417
                     /gene="rpsQ"
                     /locus_tag="PKL93_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            57477..57845
                     /gene="rplN"
                     /locus_tag="PKL93_01370"
     CDS             57477..57845
                     /gene="rplN"
                     /locus_tag="PKL93_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNQ69947.1"
                     /translation="MIQLESYLRVADNSGARMIKVIQVTGGYRKRSGTVGDIVVAAVR
                     DVAPNTDFKKGDIVRAVLVRTAKEIRRPDGTYIRFDDNAAVIIDKQNQPRGTRVFGPV
                     AREIRDKGFSKIASLAQEVW"
     gene            57859..58179
                     /gene="rplX"
                     /locus_tag="PKL93_01375"
     CDS             57859..58179
                     /gene="rplX"
                     /locus_tag="PKL93_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNQ69948.1"
                     /translation="MSRVKKEDTVMIISGKDRGKKGKVLKTFPSEKKIIVEGVNFTKK
                     HQRPTNQYREGGIIERESPIYVSKVMVVCPNCNKPTRVAHKILENGEKVRSCKKCGEI
                     IDKV"
     gene            58196..58756
                     /gene="rplE"
                     /locus_tag="PKL93_01380"
     CDS             58196..58756
                     /gene="rplE"
                     /locus_tag="PKL93_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNQ69949.1"
                     /translation="MAYEYVPLKDRYENEVIPVMTKEFGYKNRLEVPRVSKIVVNMGI
                     GEGSRNADLLDLHGKELMTIVGQKPVVTKAKRSIANFKLRDGMPVGLKVTLRGPRMYN
                     FLYKLINIVLPKLRDFRGVDPDSFDGRGNYAMGLPEQLVFPEIVPDQIKRVQGMDIIV
                     VTTARTDEEARRLLALLGLPFKRVMV"
     gene            58769..58954
                     /locus_tag="PKL93_01385"
     CDS             58769..58954
                     /locus_tag="PKL93_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNQ69950.1"
                     /translation="MAKKSIVEKWKREPKFKVRKYNRCNLCGRPRAVYREFGLCRVCF
                     RQLASEGKLPGVKKASW"
     gene            58968..59372
                     /gene="rpsH"
                     /locus_tag="PKL93_01390"
     CDS             58968..59372
                     /gene="rpsH"
                     /locus_tag="PKL93_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNQ69951.1"
                     /translation="MWSDPIADMLTRIRNANAAFKESVDIPASNLKKKLLDILKAEGY
                     IDDYKYIEDGKQGVLKVFLKYKGDRRHKVNVISGIVRISKPGKRLYVNKDKLPKVKSG
                     MGIAIVSTSSGVMTDKQARSQGVGGEVICYVW"
     gene            59385..59939
                     /gene="rplF"
                     /locus_tag="PKL93_01395"
     CDS             59385..59939
                     /gene="rplF"
                     /locus_tag="PKL93_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNQ69952.1"
                     /translation="MSRLLKHSIKIPAGVSCKIEGSVISVKGPKGELKQELLPLVKIE
                     ADEKEIWVKSNEEVVIRKSDYKRLAKYAGTFWSLINNMIVGVTQGFVKELEIVGVGYR
                     AQLQGKKLVMNLGYAHPVEIEPPAGVEFEVPAPQAIVVKGIDKYQVGQVAANIKRWRI
                     PIVYSGKGIRYKGEQVRTKVGKKV"
     gene            59957..60325
                     /gene="rplR"
                     /locus_tag="PKL93_01400"
     CDS             59957..60325
                     /gene="rplR"
                     /locus_tag="PKL93_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNQ69953.1"
                     /translation="MFKHKDKKEQRRKRHLRVRSTVSGTPERPRLAVFRSEKHIYAQL
                     IDDSKGITLVSASTVDKDVKASIEKSWNVDAAKKVGELLAKKAKDKGISSVVFDRGGF
                     KFHGRIKSLAEGARAGGLQF"
     gene            60330..60944
                     /gene="rpsE"
                     /locus_tag="PKL93_01405"
     CDS             60330..60944
                     /gene="rpsE"
                     /locus_tag="PKL93_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNQ69954.1"
                     /translation="MKKMAEEMKNDRNSKSSREGRRNPRASRNAPVEKQVEDNEFEER
                     IIEIRRVTKVVAGGKNLSFRVVAVVGNRDGKVGLGIGSAREVPTAIRKAVLEAKKNVT
                     EVAVRNETVPHETVGRQDSARIMLKPAGPGTGIIANSPVRAVVELAGVKNILTKSLGS
                     SNTLNMARAALNGLLSLRSPQDFAKLRDIPLKTVFHGFSEEAGQ"
     gene            60944..61132
                     /gene="rpmD"
                     /locus_tag="PKL93_01410"
     CDS             60944..61132
                     /gene="rpmD"
                     /locus_tag="PKL93_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNQ69955.1"
                     /translation="MAKSLRIRLIKSPIGYNRRQLKTVKALGLGKLDSEIVQPDSPQI
                     RGMVNSIRHLLAVEELDD"
     gene            61146..61592
                     /gene="rplO"
                     /locus_tag="PKL93_01415"
     CDS             61146..61592
                     /gene="rplO"
                     /locus_tag="PKL93_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNQ69956.1"
                     /translation="MAFKVEDLSPTPGSRTSEKRIGRGIGSGMGKTATRGHKGQGRAT
                     GKVAARFEGGQTPLFRRTPIKGFKNRGAVVYATVNISTLEERFESGSEISPEILVQRK
                     ILKDLKDGVKILARGELTKSFIVKANAFSATAKEKIESAGGKAEVI"
     gene            61595..62896
                     /gene="secY"
                     /locus_tag="PKL93_01420"
     CDS             61595..62896
                     /gene="secY"
                     /locus_tag="PKL93_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNQ69957.1"
                     /translation="MWNALKNAFKIPELRDRILFTFLALAVFRLGVYIPIPGINIQAW
                     SAYFGTLSTGGAGGFIGFFDVFTGGAVEQFSIFLMSVTPYINAQIMLQLLTAVVPSLK
                     EMLKEGEEGKKKYARYTRMLTIGLAGLQGFLISFGLSSNTAILAIPSRLLFVLLATTT
                     LIGGTMFLLWLGERITERGIGNGISVLIFGGIVARYPASIMQVVVALSPLQWGILLAI
                     ALFTVVAVIYVQMGERRIEVQYARRVTGRRVYGGVSTHIPIKVNQGGVIPIIFSSAIM
                     MLPQFIATAFPEGSGGRNIMQSLFAQTSPLYILLYGGMVFFFTFFYSSLVFDVREVSD
                     NIRNYGGYIPGIRPGFSTQQYIQRVLNRVVFMGAVFLVVIALLPLVIGGIFSIGGLAI
                     GGTSTLIAVGVAIDILQQMETHLMVRHYEGFVKKGKLRGRR"
     gene            62898..63557
                     /locus_tag="PKL93_01425"
     CDS             62898..63557
                     /locus_tag="PKL93_01425"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNQ69958.1"
                     /translation="MNVILMGPPGAGKGTQAKRIAQIFKIPHISTGDMLREAVAAGND
                     LGLKVKEIMDKGLLVPDDLMIDLVRERLSREDTRNGFILDGFPRTVEQAAALDEMLED
                     LGRKIDVALLVNADEEEVVKRISSRRVCPECGKVYNLLTIRPKVEGRCDNDGAELIQR
                     DDDMPETVRARYRVYLEKTKPVIQYYSSNKSQFLEVDGTGEIDVVTDKIVEHLESTRN
                     G"
     gene            63550..>63808
                     /locus_tag="PKL93_01430"
     CDS             63550..>63808
                     /locus_tag="PKL93_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ69959.1"
                     /translation="MVRLKSPEEICKIEIAAKIVAEVLAEMESYAVEGASALDMEKAA
                     EELIHKRGGIPAFKGYSGYPYTLCVSVNEEVIHGFPLKEKIF"
     assembly_gap    63809..63908
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <63909..64457
                     /gene="map"
                     /locus_tag="PKL93_01435"
     CDS             <63909..64457
                     /gene="map"
                     /locus_tag="PKL93_01435"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNQ69960.1"
                     /translation="VSVNEEVIHGFPLKEKIFVDGDIVSVDCGVNKDGFIGDAAKSFV
                     VGSYRSEKDKLLLQRTEEALLKGIEMAVAGNRVGDIGYAVQNCVESAGLSVIRDYVGH
                     GVGLSLHEDPQVPNYGRQGSGMLLRTGMTLAIEPMVASGHYSLEILEDGWTAVTADRS
                     RAAHFEHDIAILEDGPKILSSL"
     gene            64470..64715
                     /gene="infA"
                     /locus_tag="PKL93_01440"
     CDS             64470..64715
                     /gene="infA"
                     /locus_tag="PKL93_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNQ69961.1"
                     /translation="MSDKSDIIKMDGTVVESMPNATFKVELENNHTVLAHISGKMRKN
                     FIRLIPGDKVVVEVSIYDLTKGRIVYRKRIDKGGETN"
     gene            64712..64828
                     /gene="rpmJ"
                     /locus_tag="PKL93_01445"
     CDS             64712..64828
                     /gene="rpmJ"
                     /locus_tag="PKL93_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNQ69962.1"
                     /translation="MKVRASVKKRCEHCKVIRRNGRIRVICEKNPKHNQRQG"
     gene            64841..65209
                     /gene="rpsM"
                     /locus_tag="PKL93_01450"
     CDS             64841..65209
                     /gene="rpsM"
                     /locus_tag="PKL93_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNQ69963.1"
                     /translation="MARILGVELPNNKKTFVALTYIYGIGYTRSNEILSGTEIDGNKR
                     AKDLTDEEVSKIAKYINEHYKVEGELRTEVDRSIKRLIEIGSYRGYRHRNGLPVRGQK
                     THSNGRTRKGSRASKIRKKS"
     gene            65227..65613
                     /gene="rpsK"
                     /locus_tag="PKL93_01455"
     CDS             65227..65613
                     /gene="rpsK"
                     /locus_tag="PKL93_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNQ69964.1"
                     /translation="MAKRPAAKKKRKLTTDRGVVHIKSSFNNTIITLTDPEGNTLMWT
                     SGGTAGYSGSKKSTPYAAQLGADKIAKEAIKLGITRVGIEVKGPGSGREAAIRTIQAA
                     GLTVETLKDITPLPHNGCRPRRRRRV"
     gene            65630..66256
                     /gene="rpsD"
                     /locus_tag="PKL93_01460"
     CDS             65630..66256
                     /gene="rpsD"
                     /locus_tag="PKL93_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNQ69965.1"
                     /translation="MARYTGPVCKLCRREGFKLYLKGERCFSPRCSQVKRPVAPGQHG
                     ASTRKLTQYGMQLRSKQVVKRIYGVLERQFRRYFEAALKKNEETGTALMKILESRLDN
                     IVFRMGFASSRRQARQLVNHGHVLVNGRRVNKPSFNLRVGDIVEIKEKSRSVLPIKEA
                     AEAAKERTAYPWVEVDYETFRGTYLRYPVTEEVEIPVDLQSIIELYSK"
     gene            66326..67330
                     /locus_tag="PKL93_01465"
     CDS             66326..67330
                     /locus_tag="PKL93_01465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNQ69966.1"
                     /translation="MPKNLRIEEEREDDNSYFTRFILSPMERGYATTIGNSLRRVLLS
                     SIPSMAITRLKIPGKYHEYDVIDGVKEDILEIILNFKKVQLKPALEFSDAVKLTLETA
                     GPGVITAGDIKTPTGVEVVNPSQYIATLNTDSVIYIELFAEIGRGFVPVSEMEIEHDV
                     EMIYIDGIFSPVLKVNFQTENVRVGKRTDYDKLILDVWTKKSITPSDALLRATDILIR
                     HFEVFSNGLGKPGQGLTSSALKQEEEEIISEEAEVTVETPQVEEQDSNSLGAKKVEEL
                     DLSVRSLNCLKRDKINTIGDLLDKSEADLLRIRNFGEKSMLEVVKKLREKFNIVLKKE
                     "
     gene            67352..67834
                     /gene="rplQ"
                     /locus_tag="PKL93_01470"
     CDS             67352..67834
                     /gene="rplQ"
                     /locus_tag="PKL93_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNQ69967.1"
                     /translation="MRHRVSGSKLNMPHSQRVALMRNLARELFEHGTIVTTVTRAKEL
                     RPFVESIITKAKKASLLSVEISSKGPDSTETQALKSRNLALHREINRNFNDRKLVKKI
                     CDEVAVKYHERNGGYTRIVKLGMRRGDASEMAVLQLIDNEEKQEKKPEKKAEKKQDKK
                     "
     gene            67873..68529
                     /gene="fsa"
                     /locus_tag="PKL93_01475"
     CDS             67873..68529
                     /gene="fsa"
                     /locus_tag="PKL93_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNQ69968.1"
                     /translation="MKIFLDTANIEDIREGMKLGLVDGVTTNPTLVSREGVKFEDRVV
                     EICETVKGPVSAEVTSTDYENMVLQARELASLNEHVVVKIPMTRDGMRAVKTLCGEGI
                     KTNVTLIFNSLQATLAAKAGATYVSPFVGRLDDIASNGMEIVEEIVQIFANYGYSTEI
                     IVASVRHPMHVLEAALMGADIVTIPYEVLLKLFNHPLTDIGIERFMDDWKRYQKQQGK
                     "
     gene            69049..70311
                     /gene="rho"
                     /locus_tag="PKL93_01480"
     CDS             69049..70311
                     /gene="rho"
                     /locus_tag="PKL93_01480"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNQ69969.1"
                     /translation="MDISQLQAMPIREVYKLARKYDVQGYTQMAKKDLIFAVLKAQTE
                     SYGYFFDQGVLEITDGGYGFLRTLDNNLLPSSNDIYVSQSQIRRFNLTSGDTVAGQVR
                     PPKEGEKYFALLRIEAINHKAINFAAERITFQNLTPIHPEDRLITETDSHIMSTRMVD
                     LFSPIGKGQRGLIVSPPKAGKTILLKELANGIAENHPDTIRIILLIDERPEEVTDLRR
                     STNAHVIAAPFDMHPEKQIRVAEMTIEMAKRLVEFNYDVVILLDSITRLARAYNLYVP
                     PSGKLLSGGVDPSALYKPKYFFGAARNIEEGGSLTIIATALVETGSKMDEVIFEEFKG
                     TGNMELILSRQLANKRMFPSINLTLSGTRREELLLPSDILRKIWILRRMLSSMSEEEG
                     LRLIMEKLRGTGSNDEFLDLIEMEKKQY"
     gene            70325..71275
                     /locus_tag="PKL93_01485"
     CDS             70325..71275
                     /locus_tag="PKL93_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ69970.1"
                     /translation="MVRRVIGIDLGGTETKIGIVEEDGKIVEKKVIPTRVSEGRTTVV
                     TRIGEAINELLVQSGIDSKQIIGIGVGSPGSIDHDTGTVLFSPNLPDWSGFGLAAMLE
                     RVTGIRTFVENDANSFILGEWAFGEFKGSQHMVGLTLGTGVGGGVITHGILMTGSKGY
                     GGELGHTIVEPEGPVCGCGSHGCLEALASATAIINLAREYSKRFPQSTIFASPEINAK
                     VVFDAAREGDLAATLIVERATRALAIAIGNFIHVFNPEHIVIGGGISRAGDLLINGIR
                     EKLPAFVMTSFNGTFSITLSKLVENAGITGAASIVFYRTS"
     gene            complement(71260..71937)
                     /locus_tag="PKL93_01490"
     CDS             complement(71260..71937)
                     /locus_tag="PKL93_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNQ69971.1"
                     /translation="MNIEYIHSWSMEPVQAIDLQKKLRDKLTFEDYSREPRLVAGIDV
                     SFPFREIALAVIVIMEFNSLSIVDHFFSLKKVETPYIPGLLSFREGPAILDALSRSPE
                     VDLLFFDGHGIAHPRGIGIASHIGLFVEIPTIGVAKSLLYGKVESLPREKGQSSRILS
                     PGGRLLGYALCTRTSVKPVFISPGNRITAESALRLSIRTIGKYRIPEPTRQAHILTQR
                     LKNQLVL"
     gene            72042..73145
                     /gene="dnaN"
                     /locus_tag="PKL93_01495"
     CDS             72042..73145
                     /gene="dnaN"
                     /locus_tag="PKL93_01495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNQ69972.1"
                     /translation="MKFVAARSEILTRLESVSGAVAPKSVKPILSGIYFSMKDDSTVK
                     LQATDLETAITTELRPKHIDGRCDFVIDARLVLEIVRNLPEGEVVFETEDTNVIIREG
                     SARFTLPTMDPDEFPIVEPTSGGLEFTVSVSSIELMIDRVIFCAARDEFMRNLNSVYW
                     EFEDGYLRLVAADGFRMALSEERIDLSIDDHFLLTLKSMKDLQNSLKAAMSDLLKVTY
                     DGSRVQFLFDGTEIVTKVVDAEFPDYKKVLPKSFKARLVLSTEVFSDAVRRASIAARL
                     GSDSVKFEIDDEQFKIVARSPDHGESIEILDANKEGDNIVIAFNPRFLSESAKKIDSD
                     TVELNFVDSNSPLQMNPVDVQGYTYVIMPIRLI"
     gene            73154..73609
                     /locus_tag="PKL93_01500"
     CDS             73154..73609
                     /locus_tag="PKL93_01500"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNQ69973.1"
                     /translation="MGIGYDIHSIKPGDRGMYLGGVKVSDTFYLVGHSDGDALCHAIV
                     DAILGSTSSGNIGISFPEDEKNRDRRSIEFLMEVAESIRGRWRILNVDAVVVIEKVRL
                     AGFVSSMTENVARALGIESEQVNVKPKSGNGSSPGFVQVHAVCLLEKVG"
     gene            73613..74815
                     /locus_tag="PKL93_01505"
     CDS             73613..74815
                     /locus_tag="PKL93_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ69974.1"
                     /translation="MPEFRYEVINIKGKPEKGKLSANSKLEALQILSSRGFIVSSIKQ
                     SSTARNYTKASLFPASQKEVSIFSRQLATMVSSGVRIRDSLQVLSTQVLFSRRFRKII
                     SRVVLDIEGGMSFSESLEKTGVFEPLFTNLVKAGEAGGVLDESLERVADFYEGMVELQ
                     NQVKSAMAYPLFMMVFAVGIVGMISFFILPNLISAFGGNYEPEGTMAILLKMNDILKN
                     HWPILLVLLLALSIGGFFFFKSKYGNIVKETLGKLIPPVRNLRNMTAMERFTRTTAVL
                     VASGVDLPTVLELAGEVSQSPRVMKAVGNAIVSIKGGESINAALERQKVFPPIVVSMV
                     ATGEETGKLDEVMFKVANFYHMQVQNALKKLVSLVEPIMILFIGGFIGFLAITIYGAV
                     FAMEQSIG"
     gene            74812..75192
                     /locus_tag="PKL93_01510"
     CDS             74812..75192
                     /locus_tag="PKL93_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69975.1"
                     /translation="MSFTELLCCLVVVSLCISGVVLVSSDIIASLKIVLSKTAFDSFI
                     ESQRLEAVVRSRPVDVFYDFRSRRVSSTTGMVFEDCLWENPENFRIRFNGDGSIAILS
                     GSTTLNFADGSVLTIQPVTGKVSY"
     gene            75209..76246
                     /locus_tag="PKL93_01515"
     CDS             75209..76246
                     /locus_tag="PKL93_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69976.1"
                     /translation="MFQSMITAVNSDEGYTEIVRAVVEGRYLKPVFSVLKLGSDSLEE
                     ASRVFEENNSLPADEIFVTAFHPKDVVFQHLEVPRVKSVKNLVSVASFKAASQFSLPP
                     DEVNVACLNSLSSLRTGLVPAFIITKKRALYEFIGELTGKNFPEPNIVDVKPFSIFKI
                     AQTSVFEGNNIVFIVDKSYSLLLTLRGEEIVGLNYINDGFDEVASDFKEDNSLSPNTN
                     MQREFLIGSKAYYDSLIEEASERLESMISYQLRMYLTNTLSNSPNTSAADESLFNRFF
                     VAGQSSLSTAVYSRAFQQILGTETEVASMPLKMKETSGITYTSAGLLMRGGELLGRRK
                     LVFKEEAGLKT"
     gene            76188..76769
                     /locus_tag="PKL93_01520"
     CDS             76188..76769
                     /locus_tag="PKL93_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69977.1"
                     /translation="MSYLVDVNLYSRKRRVSRLKYSLIVLLIIILLIFGSRFFVTQAF
                     EIRLRQVSRMKGYLFDNTGTYLTGNLKEDIDMMYEKKQEYIAIRNKLSRQVNDLRKHV
                     DLKSVEMDVFRTIEAYLEDENAMKTIASRIQFESGVGNSFYYLIFEGKEMFFPTPHTT
                     PTLLARPSVRGNLDFAELYSLQLVDLEVGDLNE"
     gene            76762..77358
                     /locus_tag="PKL93_01525"
     CDS             76762..77358
                     /locus_tag="PKL93_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69978.1"
                     /translation="MNEKNGFNFEEMKAPAGAEPFEGSDSFFIHRDYANKLNRRLSFL
                     DKKGINSKLAFWSLIALTVLGFLFLVILYSYSTFENTSLLIDEVNGLESAYLEVQLLS
                     RVRNEALGSMSTRSYLNDLEEKIPIGSKPGDYNGIIASGNSRDILDLVEMIVNEPRIL
                     VKKLELRSNLGFPILPESNIPSNLSLELKIDLNVTGIF"
     gene            77373..77813
                     /locus_tag="PKL93_01530"
     CDS             77373..77813
                     /locus_tag="PKL93_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ69979.1"
                     /translation="MEKYVMVIVIAIFLIVTGGVILTFLLEPVEIADVAASRIKIDPI
                     NTDRVVSSVSLENHFLPLYASPSEVISSMLEVGEEAEGVRYVAYYRLNKIDYAVLESG
                     QSQVTVRVGEQVAPSYIVYGITEFAVLLNDTSTNSFVVVKYFRS"
     gene            77842..>83767
                     /locus_tag="PKL93_01535"
     CDS             77842..>83767
                     /locus_tag="PKL93_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretory pathway, component HofQ"
                     /protein_id="HNQ69980.1"
                     /translation="MRRIAVLFLVLGFFCIGFSNQLNSVIPSMDAESVTVTLIFMNEV
                     TDYNIQSNPSKTIYSLALDGVSGRDMNLPLRMGPVEGLWTRQDPKRLSVNVALLIPAI
                     DEPQVTIRENIVTLRFFRSNIDVDIEKFTTYGMTLSTAMTYLFSEEMLDLSYIISPSV
                     RDEEVVVGFSLAMPEDILRNILTSLGDRVAYSYLTDGTFYLGTPEEVQSLVNAFWKTY
                     IGVEFKLEGETVSEQIERLRKTLPVNSFIEYLPNEASLMVFGDLQTHMSLSAALTTNT
                     VTKEFTIPWEITDVEGTPLNSYLDLARKLNATLFSESLTIISIPEFNKLILSGNDSQV
                     NALYEYLELYRENVTSEDLDSSKARKTITLPDNFFLIEDVLKLGSVGGSGLTEGNTLV
                     GAILSILQSYAPYEEIAVDRAFEPIGKVTFELPNYLVPVLEKTVDSFSGISAEVSYTI
                     VNYHLTIEESVRFQVERITGALIEPLGERKGYILKGSKSSIGTAEYLLSLFTGITFED
                     YSSTYLELKANDSFEEVSAFLRTFYSAQGLVQDEYTVEQIADRLVFMYAPSEIMEKAV
                     LELEQHERQIYRVTKESILEIAPEIYSSGLGELLQTILVENETADFIQSAGLLVVSAY
                     PERLAEIETIIEERIPRIKEMLENRMSSQENRLSLQIEAIPGWDIEKFRTYLNDFLGT
                     ELFSEITLTTSGSGYIVIAAESVLEAISAEAEKLRGFEEPSYRLETSIPSLAQFELLL
                     QRLGIAVTIVPVDNQFMIVGSKVNVEEASRLVRQLSSGLPVQGEVSESETVYEYRFID
                     IPYSEVQGISDLLDKLNLSVEFVNTPSGVAIVATEDRLKEAMVVIDSILSRIETIETG
                     PRSYSLIDIEPSLVDSLDEILSLLGYEIDLVESPAGVIGIGVEAQLAKAKKIVEEIMN
                     KEASILPQQGDIESEKSYLIIFSKPEVEIASLNSMIDVFGMDLVITPIYDKIVVVGDS
                     EDLKLFNGLYDQILTTESVEGAFVSREIAGVPGWDSERLLSYLGVVLGPEKTANISFV
                     QTAKGYIISCPESIIERVENEFERLRGIESPSYSIESKMPQIDEINSLLSRLGIIVDI
                     LEVDDYFVVVGSEYNVARAKELISKLVEAIGEDPESKETVFELSTIESKDSQVFGEIF
                     SSIGIDVRLLESPSGVLVVGREEEVSKAIETSKSILQKRPSTALAQAVFEFVPAAFEE
                     IGDYSLILEKMGIDAELLPSPSGVIVVGSSEEVSKARTAVQTIHDQKQAFDSTEDSKN
                     VSTFVRRVAGWSDEVFTSYLSDFLGEDLYSRVSIAPTVSGYIAVGPIDLLETIEAEVA
                     RLNEIQDPYFIVKQGLTPLDQLELLIEALGIRVEIIPVEDKYLIVGNHDSVVQTGELI
                     DQLIDAIYPEGKPTEQTATSKTFIFLPIEPEDIAPLETVMEKLGIISEFVQISNGVIA
                     VGSTEELSRSLDVVESVMAHSESVTAELEMDYRFVDVLKDSIDQLLPIIERLSIGVEL
                     LDTPAGVVAIGAEQDLDRAEELIKAITSRDTKEVPLQEKYESYLLLPLSAGITVESLQ
                     PALELLAYRVFAVSVADKLIAIGSEEDLLKFKALYMQLQSQEVDREERISLEIKMIPG
                     WDLTRLNEYLRLFLGADEFERISIVSSSTGFVALTPRALADVVEAEISRLREFEDPAF
                     TVFERVPPVDDLEGLLVRLGVLVDLVEMGSSTIVIGSGEDVRQTGIIIEQLMESVKFD
                     VASPEAMEYSVLDIAQDDIGSFSAILESLTIDVSLISSPSGAIAIGSHQAVARAKTVV
                     EDILSRRTTPQVYEPTYAFTGIQSADIAIYQTILDKLDSGVELLPSATGVIMIGEGDK
                     VQEAVKTVNAILEREALFDESEGQPDRVSNVAAIVPGWNDEKYASYFADFLGKDDFSR
                     ITITPSSSGYIVVGPQEVVSKLVKEASRLAGIEDPFFVIENSLPAVEQMDSLLERLGL
                     KVTILPVEDKFLVVGKK"
     assembly_gap    83768..83867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <83868..>87535
                     /locus_tag="PKL93_01540"
     CDS             <83868..>87535
                     /locus_tag="PKL93_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretory pathway, component HofQ"
                     /protein_id="HNQ69981.1"
                     /translation="LKVTILPVEDKFLVVGKKESVEEAVELIRLLSNGLEAPSSSRKY
                     DFQIMPASEGAFDTIRQVLAELKIEATALSFGDNVILVGYDEDLESAAKVVNSINERT
                     VTLETDKELKYSFTEIPNDRINEFSAILDSLEMDVQLLSSPSGVVLVGTEEESAKAND
                     VILSVLSKAEEDEMVESRFFNNPAGWEDGKLVTYLRSFLGEKDWAKISVTPVQSGYLI
                     VGPTAILDRIHSETVRLSGLEKPHYEIVDTVPSIQNLQGMLEKMGLAVTVVIVDGRSM
                     VIGPEDDVIQTVRIFSELSEGVVLPKGEEPDVFNFVEIPTEEVESFRAIFSRLGIRVD
                     LLNAPTGVIAVGQGEEVAKAVDTITSILSRREVQVVDGEKSYLVVEVADGFDLAAAQS
                     IVSAFNINVFPLSVAGKLVVIGSENEIMRFAEIREDIISEHRVTVIVPREVTLEELTG
                     IVDTLEMEISVLEIRDSFVVYGKEKDIEQIRAILSELVSEKPSEEVRHSRIFTGISLS
                     TDFLNKLLSSMNSSLTIYGEGDKLVAVGTEDDIEMFEGLLKEIGSAPSTEELTAQFYP
                     RIEGWTGETLSGFFEAMNLAVQVFHENSQGYLLIGPESELSKLPDIMEGLELKTKKLT
                     TLYPVPSGMTFEELREILISAGFNLSYTKLGQSMSISGLEEDVELAVALLDEVIRSNL
                     GKGISYRLIKLPADLTLEKFEKIMGDMALGISSVQVDGEVMLIGSDSDLRTANEIVEY
                     LTPEGTPIEDEVVYSVIPISEELSMTEIAALVEKLSLNVEILPVGEDLFVIGTQRNVD
                     SFRELVNSISATLGIEEVQSPEYTIVEFVEEISLDQFRELLASLNISVKIVEVGDYLV
                     FIGSGEDNLEAAELFTRFETKDTEPESEEIEFEALSFPEGFDINSLTTVFEKLDLRVD
                     INAVGNVLLIIGTRESIENAVTIIEKLVQVSTGDSGEGNVFKVLESKEGVSSELLSQL
                     FRLIGVEVEILESETVFVFVGSEADVEMAVDVYDSLGESAGVDEGPLSYTITILPKNL
                     TPSQLQTAFTSIQIPVGVIEAGDYAIIVGTKASLARAQELISSLRIGIDASGTSADGS
                     VSYITFALPEGTEINQFESIAGLMELKLKFEPVGERVFMIGTAEDIGEFEQVLSGLVN
                     ERSGIVNEFRFVKKLSGVDAETLKTYLLAKEIELSGVFDVSGGYLLIGSADSLDVAQS
                     IIDYLLDNQQVHFAYIDLP"
     assembly_gap    87536..87635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <87636..89303
                     /locus_tag="PKL93_01545"
     CDS             <87636..89303
                     /locus_tag="PKL93_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNQ69982.1"
                     /translation="EAAEIQLRSIIEYSGRLIGIGTQVGLENAREALSILAVDSKRES
                     VRVEKSLVSDNQLQEIISKLSLEVDYIDLDRQWLLIGDPESLMIITRTIEEAAADREI
                     SRYITDLTVNPQELADLLRESIEGITVQTFADLQMLLIKSKSSALLDAAEEMVRDVQE
                     SRKAIDPLEKGVVVKDSMVSIRVTDQDLEALLRTVSEKLGISLLFVDKIVENITMSIE
                     DLSWDKLVKVINATKPVEIVRIDDIYAVTKKEQKAGEEPTDVELVYRVYHNVEELTRL
                     IEFYGGEVLSDPVNGYIVVRGLAKSRVDNIFDEIGSALSQPKKQVRIETRLVDESLID
                     EIQREIKTSIGINAPEILIENGSIDLDFGIIDYLDITQLIDDLVNTGTVKIEADLSDR
                     DNDSDLISSPSIVSMSGEEAKIHIGDTIPYLVRTIEYVDGNPVEIETIEYLNTGVELR
                     ITPTVNDDGKILLDLFIKVSEPEKYVDGTRTLYGEKTREANSRLIISDGNTLTIGGLV
                     ANKDTVTVNKMPFLSDLPFIGKLFTTENKTSDKRELVIFITAEVVEP"
     gene            89300..90127
                     /gene="amrB"
                     /locus_tag="PKL93_01550"
     CDS             89300..90127
                     /gene="amrB"
                     /locus_tag="PKL93_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNQ69983.1"
                     /translation="MIRRPVVSGKFYADNEEELKSQIRNCFFHPVGPGSLPHGWEKNE
                     GPIGLIVPHAGYMASGPVAVWSYWKAGALVKPSTILIVGPNHTGLGTKLSLWLEGAWE
                     TPLGSVEIDHDFGRSLMAESNGMIMPDTSAHLYEHSIEVQLPFLQFLYSDFKIVPLIM
                     TDQRVEVALSLSEIIKKELMGRNDVLVIASSDLNHYEAHEVTMEKDNELLSLILDGKY
                     RESYELSQEKRITACGLGPIVAVRNSFESMEVLCSTTSGEVMGDRYRTVGYVAALLS"
     gene            90214..90714
                     /locus_tag="PKL93_01555"
     CDS             90214..90714
                     /locus_tag="PKL93_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ69984.1"
                     /translation="MKAVISLLIVFLMSSLGLASLIIDGIHYSGFGSEDKLVYDGINV
                     AYVLSQTGKNSLELGGNFSFVSPVSSEDFYINGHFNTLLKLFAGQAYDLTEESAVGLR
                     VVSRAGLITTNVDKFGIFFSITPIAYVRVGFLSFGAAVGLELEKFFGMETLPVFHAGG
                     EISYRF"
     gene            90726..91160
                     /locus_tag="PKL93_01560"
     CDS             90726..91160
                     /locus_tag="PKL93_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNQ69985.1"
                     /translation="MRELAIVKELKGDVVLLEKARTEACSTCGSRSSCSISSSDKKIT
                     IRALHNGVEVNPGDTVEIEMSNMSATRVSMIVYGIPLAVFLVTLIVMNMVLKSEGLAL
                     VVAFASIAVVYALIALYDRKNREKLMPKIVRKVELPDGFIVR"
     gene            91141..93447
                     /locus_tag="PKL93_01565"
     CDS             91141..93447
                     /locus_tag="PKL93_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNQ69986.1"
                     /translation="MDSLFDDSIRLLEFDSVLADIAQKATSKYGKERVKSLRPKAEDA
                     EILYTRSSEFNEILLREGEPPFTVFHDLSDYITKVKRGFSLGGEELFRSAVTMDNVCR
                     LREFFDRVSPDFPAVYEVAALLSCERGFIGEVRKKISEDGQIKDNASPLLKEIRNELK
                     NLNRNLRGRLDSIMNRYKDSLTDSLILTREGRYVLPLSASKKNEHQGVIHGASGSGAT
                     VYFEPQELITLNDSMRILLSREEEEIRRILRELTTLFFSTFERLEPSLEALGVLDGLY
                     AACRYSKSRRGVFIEPSPSRHFLLKDARHPLIDDEVVVPITFTMEEGVNGVFITGPNT
                     GGKTVAMKTIGLSVLLMLCGLPVLADGLSKIPLFSRLFADIGDEQSIEQSLSTFSSHI
                     SRIIRIIRNADENSLVLLDELGAGTDPVEGSALSIAIIERLLERSRLILTTHLTPIKL
                     YAMERPDVVNASVEFDISTLKPTYRLLMGIPGSSNALEVSSRLGLPQEIVDRARSYID
                     SEARDLEAVIGKLHRERSLLEEERRGLDKTKRELEGRTKEFEEKLSMIKEKRYREVSK
                     EIDELEKKLGSIIKDVEMAINLSRAPGEREKVEAVKRLNNLKREVRNFGASYPEKTDS
                     QIPEVGDIVKLLESGAEGIVSEIDGDRVVIDSGKITLKVPLSRVRVVGKPVVESEMSS
                     FELDSGNSSQEIDIRGNITEDVPILIDDFLQMLKASGKKQGYIIHGKGTGKLAEGVWA
                     YLRRSKGVKSFRIGTPLEGGSGVTVVEV"
     gene            93444..93812
                     /gene="acpS"
                     /locus_tag="PKL93_01570"
     CDS             93444..93812
                     /gene="acpS"
                     /locus_tag="PKL93_01570"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNQ69987.1"
                     /translation="MNLNIRTGIDIVEISRMTESIAQRILGKDEKVIYDSLGGERRRM
                     EFAAGRFAAKEAFVKALGRKDIEFSKIQFLTDEGGRPIPSDELKQLIKNSDLTVSISH
                     EREYAVSIVILFGEDQNDNV"
     gene            93799..95796
                     /gene="ligA"
                     /locus_tag="PKL93_01575"
     CDS             93799..95796
                     /gene="ligA"
                     /locus_tag="PKL93_01575"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNQ69988.1"
                     /translation="MITFEEARKRIEELRKEIDYHAYRYYVLDDPEIPDSEYDSLLRT
                     LIDLEEQYPELKTPDSPSIRVGLKPVASFKEVVHEYRLFSLDNTYSEEEVLQFDKRVR
                     EELQIEIVRYMCELKIDGLSISVKYDNGLLVRGATRGNGFVGEDVTENIRAIKSIPLR
                     LRQDLSIEVRGEVYLPKDVFHEINRARSKGELQVFANPRNAAAGTLRQLDPREVAKRR
                     LNAFFYHVVEPSKFQLKSQSEQLQFLKSIGMRVEPNSELAEGTDGVIAFWKKWSQSKS
                     SLNYAVDGTVVKVDDLHAQEELGYTTKAPRWAIAFKFPAEQAMTKLIKVTFGVGRTGT
                     ITPVAELEPVQLAGTTVKRASMHNFDFISEKDIRLFDTVIVQKAGEIIPQIVRSIPEK
                     RTGKEKRITPPSRCPVCGGEVGREKEEDVALKCLNPTCPAKLSRRIQFFCSRDAMDIE
                     GLGEKLVERIVEAGLVKNPSDLYKLNKDDLLSLGEGIGEKTASNLIEAIQKSRSNPLF
                     KLITGLGIPGVGSKTSRDLANHFGSLRGLVSADEKELRNVSGIGDQLAKDIKKYFSSP
                     EVKKELEELLKYVNIQDEDSPGSKPLKGMKFVVTGTLPGYSRKEIQEKIIKLGGEVAS
                     SVSKNTDFLLLGENPGSKEQKARDLGIKIIDERDFEEMIKS"
     gene            95796..96920
                     /locus_tag="PKL93_01580"
     CDS             95796..96920
                     /locus_tag="PKL93_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNQ69989.1"
                     /translation="MRYFDNNATTKMESDLAERFAKLCVEEYANPNSMHSFGLREARY
                     LEEARRKIARSLSSDPREIYFTSCATESINWILRSSVLFKGRKKRIVTTSIEHKAVLN
                     TLKDLKASSDIDYTTVGPERNGIVKVAKLLDEVDEETFLVSLMAVNNVTGAIQPYEEI
                     GEELRKRNVLFHLDAVQTIGKIPIDFVSCFCDYASFSAHKFHGPKGVGVTYIRQGAPI
                     RPLITGGGQERGMRSGTQNVPGTAIAATALQRAVECTQESSSRLREYQHRIKETVEEL
                     DGVVNTPENSISNTVNASFAGIRSEVLVNALSEEGVFVGTSSACSSRGDGGQYVLDSM
                     GLDFSLASSSIRISMSRFTTEEDVAFLIEKLKKTVPLLKF"
     gene            96978..97850
                     /locus_tag="PKL93_01585"
     CDS             96978..97850
                     /locus_tag="PKL93_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNQ69990.1"
                     /translation="MLRSMTGFARVEKTVRDVNVFVELKTVNSKYLNVDVNIGDAFSE
                     LEMNVTRVIKENLKRGTVRARIDISLVNSDDLLQPDFGIASSVYNSLKSIKERFELAG
                     DVTVDSMARFKEIMRSKPSEDLAKKIWSVIEELLMEAISVLNSDREREGENLASALNE
                     YLCELESIGRELQDSSKDMVLYYRDFLKKKVEQISDSQLDDNRLEQEVALLAERADIS
                     EEIVRLISHIESFREVMASEKESGVQLDFICQEMHRELSTIASKSKKLAITNLSVEGR
                     TLVNKIREQVQNIE"
     gene            97854..98162
                     /locus_tag="PKL93_01590"
     CDS             97854..98162
                     /locus_tag="PKL93_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNQ69991.1"
                     /translation="MFHLYGLINVGFGNVIIGDRVIAIVNPESAPLKRLKEVAKDEGK
                     LIDATYGRKTRAIVITDSNHIILSAIQPETIASRFMQTFSDIEKLLEDIRVSGQNFEE
                     "
     gene            98159..98782
                     /gene="gmk"
                     /locus_tag="PKL93_01595"
     CDS             98159..98782
                     /gene="gmk"
                     /locus_tag="PKL93_01595"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNQ69992.1"
                     /translation="MKGIVFVMSGPSGAGKTSILREVLRSNNNLDFSVSYATRERRPE
                     EVQGKDYFFVTPEEFKRLQKEDEFLEWAKVHGNYYGTSRHQVSKSVDLGRDILLDVDI
                     QGAMSLMSKLRDAVYIFVAPPSYEELINRLQSRGTEDTQSLRTRLEDAKWELEQVKNF
                     QYLVINDNLKHSVSQFEAIITAERLRVDRMVREKGERFLFEEKTYDN"
     gene            98772..99005
                     /locus_tag="PKL93_01600"
     CDS             98772..99005
                     /locus_tag="PKL93_01600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNQ69993.1"
                     /translation="MIINYDLLLKRIRHKYAIPVAAAKRAEDLEDFGRPKLDPSTVRA
                     AGDKITIALKELEEGYIRIRNEEMLMILVPKVK"
     gene            99105..99180
                     /locus_tag="PKL93_01605"
     tRNA            99105..99180
                     /locus_tag="PKL93_01605"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99138..99140,aa:Thr,seq:tgt)
     gene            99197..99283
                     /locus_tag="PKL93_01610"
     tRNA            99197..99283
                     /locus_tag="PKL93_01610"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99231..99233,aa:Tyr,seq:gta)
     gene            99313..99480
                     /gene="rpmG"
                     /locus_tag="PKL93_01615"
     CDS             99313..99480
                     /gene="rpmG"
                     /locus_tag="PKL93_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNQ69994.1"
                     /translation="MAKKTKGNKVLVTLKCSECGTRNYYRFKNRQKKYKLDSSKYCPK
                     CRKHTEHKESK"
     gene            99516..99722
                     /gene="secE"
                     /locus_tag="PKL93_01620"
     CDS             99516..99722
                     /gene="secE"
                     /locus_tag="PKL93_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNQ69995.1"
                     /translation="MAKAKFWKFLSEVKNEVKKVTWPNREQMISSTGAVLVILIAVGA
                     FLALLDVLFTNAIGSLLNFLTGAA"
     gene            99748..100815
                     /gene="nusG"
                     /locus_tag="PKL93_01625"
     CDS             99748..100815
                     /gene="nusG"
                     /locus_tag="PKL93_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNQ69996.1"
                     /translation="MRKKWFIIQTYSGLENSIREAIQIKIESFGFSHMFGKILVPEET
                     KLDRTNVAAEKYIVPANARLLVKDNEDVAKGDPVAEEVEIKVKNDGTVAEVKNYRVIF
                     IETADRRYTKTYYIPESAKIETGIKAGARIRQGMPLAKQGEYFCELDGKIVYTQKMKR
                     IVVDKENGEEDVYLVHPDSYDAKVAKKGNHVKRGQMFGETRRIFSKTEGRVEISDLPG
                     RKEIKIFKITRTRLYPGYVFIEMIMNEETWNLVKNTPNVVNFVSVGGQPVQLKEKEIK
                     ALLRLVGIEEYEEHMGGPVKIEVEFDVGEVVRINSGPFEDFVGKVTDLDPDRQELKVV
                     VSIFGRETPVILSLSEVEKIV"
     gene            100870..101295
                     /gene="rplK"
                     /locus_tag="PKL93_01630"
     CDS             100870..101295
                     /gene="rplK"
                     /locus_tag="PKL93_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNQ69997.1"
                     /translation="MAKKVIAQIKLQLEAGKATPAPPVGPALGQHGVNIMGFCKQFNA
                     ETSDKAGMVLPVIISVYADRSFSFILKTPPASFLLLRAAGIQKGSGVPNRDKVGKISR
                     AQLEEIAKIKMPDLNARTVDAAAKIIAGTARNMGIEIIG"
     gene            101308..102006
                     /gene="rplA"
                     /locus_tag="PKL93_01635"
     CDS             101308..102006
                     /gene="rplA"
                     /locus_tag="PKL93_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNQ69998.1"
                     /translation="MPVRSKRYIQARKSVDRNVSYSVDESIDLLKNFPPTKFDETVEM
                     HLKLGIDPAKSDQQVRGTISLPNGTGKDVRVLVFARGEQAETAKRCGADFVGSDDLVQ
                     QIQGGWTDFDVAIATPDMMRDIGKLGKILGPRGLMPSPKAGTVTADVEDAVNGFKAGR
                     LEVKNDKTGNLHLPIGKKSFDKEKLRENFVSALNQIMKMKPAGSKGRFVQRVFLTTTM
                     GAGIKVDFARETEK"
     gene            102146..102688
                     /gene="rplJ"
                     /locus_tag="PKL93_01640"
     CDS             102146..102688
                     /gene="rplJ"
                     /locus_tag="PKL93_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNQ69999.1"
                     /translation="MLTRERKEEIVESLTKAYKDSSLILFADYKGMKVENITTFRDKL
                     YENYKERAQFTIKKNTLVRLALNKAGFEESEWKDSISGTTAVLTVEDVDPIAALKIIT
                     DFNKNNKTLPLIKAGYLEGRFFTGDRASDLAKLPSREQLIAMVVGGFAAPITGFVYTL
                     NGVLTKFLYALNAIKDKKAE"
     gene            102710..103096
                     /gene="rplL"
                     /locus_tag="PKL93_01645"
     CDS             102710..103096
                     /gene="rplL"
                     /locus_tag="PKL93_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNQ70000.1"
                     /translation="MTKEELISAIKEMTVAELSDLVKALEEEFGVSAAAPVAVAAAPA
                     NGGAAAEEEEEQTEFKVVLKSFGAKKIDVIKVVRSITGLGLKEAKDLVEKAGTPEGVV
                     KENLPKAEAEELKKQLEEAGAEVELK"
     gene            103202..106726
                     /locus_tag="PKL93_01650"
     CDS             103202..106726
                     /locus_tag="PKL93_01650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNQ70001.1"
                     /translation="MRKANFGKRERWVFGRVYEPLEIPNLIEIQTRSFEWFIDEGLLS
                     VLKKYSPIKSQIQRSDLKKGEKGFSLDFVATRIGEAKHSIQECKDKGLTYSVPLYVTV
                     RITDLYTGEIKEEEAFFGYLPYMTDNASFIINGAERVIVNQLVRSPGVYFVDEPTKAV
                     SGSLPIYVAHFLPVKGAWFEILLNPNKQIMQARIDRRRKLNFFVFLKALGYENDLDLL
                     RLFENEYDGSDEDELLANVGSMILEDIKTPGGDLIAEKGTLLTEEIIARAIEADVKTI
                     KMPLPIAMKTFNALQKTYGEGMTEDDAYIELFRKLRPGEIPRINAAKSYIANLYFSPD
                     RFDFSDVGRYKVNSRLKKVYQDYLKEVEGKKVSKDTDYSEESSILTVLDVVLAARHLT
                     EIESRPELLDTKDHLGNKRVRTVGELMETEFEKAFIKIHKLLEEKLTIYTSFDKISVQ
                     SLVNVRTLMTTLHQFFATSQLSQFMDQVNPLAELTNKRRLSAIGPGGLKREHARFEVR
                     DVHHSHYGRMCPIETPEGANIGLMTSLATFAKIDKYGFLLTPYRKVQKGAVTNDIVYL
                     AADEEERYNIAHSTVDMDEKGNIVDENVEIRYAGEVRYVNREEVDYMSVSPKQIVSIS
                     TSLIPFLENDDANRALMGSNMQRQAVPVVKAEAPIVGTGVEGVAARDSGYVVLSKHRG
                     KVVSVDGRRIIVQRIDEKGKPELDERGKPIEDVYTLHKFVRTNQDTAVNQRPIVSVGE
                     IVQTGTPIADGPSTEMGELALGKNVLVAFMPWEGYNFEDAILVNQELLEDDTFTTIHV
                     EMYETIARDTQLGPEEITADIPNVSKESLKNLDESGIVRVGAYVTSQDILVGKVTPKG
                     ESETTPEEKIIRSVFGDRGRDVKDSSLKLPHGVEGRVIDVKVFEKEEVSELGSGINKL
                     VKVFVGIRKPLDVGDKLAGRHGNKGVVSNIIPKEDMPFLPDGTPIQLVLSPLGVPSRM
                     NVGQVLETHLGWLGKLTNRYFATPIFDGATEEEIMTELYEARKRFGLEDGDSEDLKTG
                     KVTLRDGRSGNPFDYPIVVGVMYMLKLVHIAKDKIHARSTGPYSLIHQQPLGGKAHFG
                     GQRFGEMEVWALEAYGAAHTLNEMLTIKSDDIKGRNDVYKAILKGINIPEPGIPESFK
                     VLIKELQGLSLDVKVFDQNNVELDVDKL"
     gene            106767..111461
                     /locus_tag="PKL93_01655"
     CDS             106767..111461
                     /locus_tag="PKL93_01655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNQ70002.1"
                     /translation="MPVSTYKRKVNKLEIDIASPERIRSWSSGEVKKPETINYRTFKP
                     ERDGLFCEKIFGPTKDYECTCGKYKGKKYEGTVCERCSVRVESKEARRRRMGHIELAA
                     PVTHVWYLKSAPSVLSSLLNIPAKDLENIIYYGSKRVIERLYVVTDPKDTDFFPGMTM
                     YQTEYEIYTKKLSFEVEQGYKVKVPHGPVTTDIDGKVRIERLDSSTERELNWVVVTNE
                     TGIEKKYPVFEGSLIYVQNDDEVHKGDTLADRFLFEDEVLSATEYKIFDEYYPGKFEV
                     ETDTESDRPIVIITEIDPTVSEETGRSIGEVLIENEYEAYKELYPGKVTCDYGAAAIK
                     KLLQSIDLEELQVAIEHELKTIPKSSARALKLLRRLKYVKDFIASSNKPEWMVMDVVP
                     VIPPDLRPMIQIEGGRFATTDLNDLYRRVINRNNRLAKLLQIGAPDIIIRNEKRMLQE
                     AVDSLIYNGRVGKAVSDKSGRPLKSLTDLVKGKKGRFRRNLLGKRVDYSGRAVIVVGP
                     DLKIHECGIPKRVAMELFKPFVLSKLLHGSESSKTARKLKKTIIEKEMPEAWEVLEDV
                     IKGHPVLLNRAPTLHRISIQAFTPRLVEGNAIQLHPLVCAPFNADFDGDQMAVHVPLS
                     AAAQAEAKFLMLSRYNVISPASGKPISMPGKDIIVGVYYLTTVDHEYEKKEKEIMDYD
                     ARLREDEEISEVEAKRKVREKALEWVDWKFSSAGEALLAYDSEYISLHDPILVQLDPS
                     SGELTLTTVGRIIFNTIVPEKLRDYMKKFNKKAIKKLIYDCFHEYGIDRTADLLDDMM
                     SLGFHYATTSGLTISINDIVVYPQKDKIIEAAQERVDQIENLYKSGFLTEEGRTEEII
                     RIWSDTTQEIMEKTYTEFEKYPFNPIYMMVDSGARGNIDQLKQLAGMRGLMADPSGKT
                     IEVPIKSNFRDGLTELEFFTSTHGARKGSADTALRTSFAGYLTRRLVDVSQTITVTTP
                     NCGTHEGIEAVELMADGVTIEKLEEFLFGRVLAADVINPLTGKVLKNEKTGKEYFRDT
                     MIRDEDAAFLSGYKAKVEVAREEVLSIAKVTRLINYTELDEDIKINDKEYKKGTHIGS
                     DLLHQLKNSEIEEIKVVAFPCVNQIFVGDPIKNGSKEMLVKYQEKIDELTAKNLQKNE
                     IEKVFVRPTIKVRSVLTCESDNGVCAMCYGMDLSNHEIVNVGESVGVVAAQSIGEPGT
                     QLTMRTFHTGGIATGQDITRGLPRAEELFEARKKLKDPEALFIEKGDSGFVKDLVSDD
                     KGRRKVYIETKTGGISEYDLSSNIKPKIEIGDKVTEGEAITTGTVRPRKLMEKLGLVQ
                     TALYLLTEIKRVYAEQGVEIHDKHFELIIRQMLSKVEVTDPGDTDFLPGDLLDIVEIR
                     KINKSIEEANSRVGENREYVIGKRLARRVLGEDEDGKIQKIAAEAEPISEELLEKILK
                     GGIKQILIYDVDEDHYQKLVDDFNPDTVIPEKNIQIMPKDPIKFKRKLLRITKASLES
                     EGWLSAASFQQTPQILTEASVAGKSDFLQGLKENVIVGQLIPAGTGLDMYANLQVEEA
                     VVPSQYQEEELA"
     gene            111569..113077
                     /locus_tag="PKL93_01660"
     CDS             111569..113077
                     /locus_tag="PKL93_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ70003.1"
                     /translation="MSKPVLEINDLKKTYEISETKALDGVALTLFSGEITTLLGENAA
                     GKTTLVKVIMGIEKPDSGSMVFKGEKYLPSGPHFANEKGLGMVPQSIKVVDKLSVIEN
                     LILGFRSNQKKVRMKDYRESLRELMKQYSLQLPLDNETASLSTGQRQKLEILRVLQNS
                     PDVIIFDEPTTLISNREKEELFEIIRRLRSLGKAIIFITHKLREAYSISDKIVVLRSG
                     KVVFSESRYKTSIKELQELLYGGEFCALRVQSPTSDESLLRVQEVSVEGSSNSEAVKG
                     VSFEARRGEILFVSGVAGNGQEELLDGIFGMRRVLSGRVILDGKDITGLKPFKLRKLG
                     LAALHYDRDNIASCKGSSIRDNLLVYSISNGEWVVEEKKHVFSARKLLRDYGVTFGKI
                     QDPISSLSGGNLQKLLLAREISSDPKVLLLGEPFRGLDVKSLESVKFLLMEAKKRIAI
                     VAFSADVDEALTIADRILIMSEGRVVFSSDIRPGLSTPDLRKYLFGAMEEIE"
     gene            113074..114108
                     /locus_tag="PKL93_01665"
     CDS             113074..114108
                     /locus_tag="PKL93_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70004.1"
                     /translation="MISRLHNLGRSLLVIAISLSAGLLLVVLMSDDPGHAARAFFLSV
                     FSNRFYFGNLLAHSIPLMLTGLAASVAFTASCFNLGMEGQVYLGALAGTSVGILISDL
                     VPGAIGIAFMFTVSFFFGASVASISAILKIRTGVSELISSLLLSNGIVLVVDYFVEGP
                     LNDRASGLAASISLPAKFRFNGLMLPSGLHTGIFFSLCAAFLLWFFLFKTREGFKIRI
                     VGKNRAFAFYAGLKTNRLLFWALFLSGGLASTAGIIDVVGIHGRVMRGFSSGYGWNGI
                     AIALIARNNPVMVIPAALFFAYLESGAQIAALEANITPEFARIVQAVIFFLITAEALV
                     PKHLGRKRYA"
     gene            114101..114991
                     /locus_tag="PKL93_01670"
     CDS             114101..114991
                     /locus_tag="PKL93_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70005.1"
                     /translation="MLEAIIRDTIKNSTPIVLAASGGLLTEVSGWLNIGLEGTILSAA
                     FFAVVVSSFTGSVIIGTLAAILVAMALSSIIFFAVRYLKANLFVVGIAVNLLSVGITV
                     TLAYNWFLSKGTVVFSDSPRPVVLSLDFLTGSGFGWLNGLSLFDLSAFVVPLFLLLFF
                     KKTSTGVKLRSCGFDEESAFYSGVKVNDLRFLAYTGSGILSGLAGAALSIPMGAFVSN
                     MSGGRGWLALVAVIMGRKNPLIVLLSALMLGFVTELSVFLQVATEISPKLLLSLPYFA
                     SFIILAISYGENKDKRFMDW"
     gene            115034..116536
                     /locus_tag="PKL93_01675"
     CDS             115034..116536
                     /locus_tag="PKL93_01675"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNQ70006.1"
                     /translation="MKLIDELRSKYSEITYLHSAAALITWDMRTYIPPKGAEMRSEVL
                     GYVSTLAFRKAVSEEMGTLLSQLAEGKIEEELTEDEKAMVRVATKAYNRVKAIPPDLY
                     HRFSVLSSKSEKVWERSRNENDFKSIAPYLEEIIDLLREMARLYGYEENPYDALLEGY
                     EEGMTAKKLRKIIEPLKSELIPFIRRIVEKGKSPDTGILKGRFSKKAQEKLSRKALKL
                     IGYDFEAGRLDETVHPFTIGIGVNDVRVTTKYLPDEFTPSLFGSIHEGGHAIYEQGLP
                     ISLIDTPLYGACSLGIHESQSRMMENMVGRSMEFLSFFYPQIQKAYPSNFRHVSLCEF
                     YRAINNVEPSLIRIEADEVTYNLHIMVRFELEEALIKGDLKVSDLPEAWNSKMKEYLG
                     IEPQTNRDGVLQDVHWPSGTIGYFPSYMLGNLYAAQMFAKAKEDIPGLEKRIEKGDVL
                     SLVDWLRDNIHSKARRYEPEKLLKIATGKELDPSYFLKYVMEKYSKIYFT"
     gene            complement(116529..116993)
                     /locus_tag="PKL93_01680"
     CDS             complement(116529..116993)
                     /locus_tag="PKL93_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV (family X)-like protein"
                     /protein_id="HNQ70007.1"
                     /translation="MEKEDLARQFDLLSRLLKVARDNPFKIRTYEFAAKTIRSNLTSG
                     KLSSQDIIALSSIKGIGKAVVEKSLEFLHGSEIQKITDLKSTLPNSIYALAAHDLIDP
                     EVLSFIWKDMGLTEPLEIVGFLVDKKESLKLSQERLSSIEALLIEIGSSKTK"
     gene            complement(117074..117391)
                     /locus_tag="PKL93_01685"
     CDS             complement(117074..117391)
                     /locus_tag="PKL93_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNQ70008.1"
                     /translation="MKIAVPVKEKSMNSIPDDRFARAKYFLIYDPETGSEEYIEPDNS
                     VAHGAGPMAVNMLTKKGISALIAFHLGGNAVQAIEAAGIAFYEAFVGTAKENIDAVLR
                     KKK"
     gene            complement(117394..118272)
                     /locus_tag="PKL93_01690"
     CDS             complement(117394..118272)
                     /locus_tag="PKL93_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ70009.1"
                     /translation="MMFQLAIVSGKGGTGKTTLSGSLSVLFDSIVMADCDVDAPNLHL
                     ILENKLIEKYEYFGGKKAEISDSCISCGICEKYCRFDAVIRGGPYSVDPYACEGCGMC
                     VAVCPADAITLKDNKSGDYFLSQSNGIPLVHALLTPGEETSGGLVAEVRKLAIKVAEE
                     EKKEVIIIDGAPGIGCPATSSITGTTYVLVVAEPTVSGNHDLARIVETIRHFKRDFGI
                     VINKWDLNTSKSSFIESWCGANGIELIGKIPFDEQVEKATIMGKPVVSIKGSEAAEAI
                     VRIAQRLKRKISGVGG"
     gene            complement(118269..119120)
                     /locus_tag="PKL93_01695"
     CDS             complement(118269..119120)
                     /locus_tag="PKL93_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ70010.1"
                     /translation="MVIAVLSGKGGTGKTTLATNLAEAISDEFEVQLLDADAEEPDVH
                     LFFEPDINQEEEIELLLPLIDNEKCTRCGKCAEACQFGALSVFDAGVMVFESLCHGCG
                     LCTFICPEKAITERPKIIGKVQTGKINKKIDFGMGILEIGEPSPVKVIRTLKKHINRD
                     RIVIIDSPPGTSCSVVETLRNVDYAILVTEPTPFGLHDLKLAVEIVREMDIPFGIVIN
                     RDTGSYTLVDEYASEENFSVLERIPFDRGIAESYSEGKLFVETSPIWKARFKSLAERI
                     LSSAGVS"
     gene            complement(119124..119351)
                     /locus_tag="PKL93_01700"
     CDS             complement(119124..119351)
                     /locus_tag="PKL93_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNQ70011.1"
                     /translation="MPWRDGTGPEGYGPMTGWGMGNCAPRDRVVGPAYPVRNYSYRFF
                     PRRRLFLGRRMGFGAGFGPGRGMGYGFRRWW"
     gene            complement(119356..119664)
                     /locus_tag="PKL93_01705"
     CDS             complement(119356..119664)
                     /locus_tag="PKL93_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HNQ70012.1"
                     /translation="MPYGSGPMRFRDGSCRFYGYGPGYGAGYGRGMGYGRGFGPGRGY
                     GYYGPYDYDVEISPEMERQDLREYQAFLKKELDMINEELREIEKRLETLQNTTSAEEE
                     "
     gene            119789..120385
                     /locus_tag="PKL93_01710"
     CDS             119789..120385
                     /locus_tag="PKL93_01710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ70013.1"
                     /translation="MGNRAHVLLFTLIAPFYDVAFKSQLKSYRKLLKEHRSLLGKGIR
                     SVLDIGCGTGALALAFSELGYEVTAVDASAAMVMIARHNLRGSGVSVVQGDFFEKLPF
                     EDSSFDLLVASYVTHGHKREGREKFYKESRRICNKEVLIHEFFPNRNPLISTVEFFER
                     SDYRKFVPKAFEELKEYFPVVDRRRLSSSTGWYICRCE"
     gene            120489..121421
                     /locus_tag="PKL93_01715"
     CDS             120489..121421
                     /locus_tag="PKL93_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70014.1"
                     /translation="MKKFIPYLFIAPAGLIIAFFMLYPLFYSFAISLFEWDLSPNMTF
                     VGLRNYSEILSSSEFWDSMLYTAYYILGVLPFSLLIGFLIATLLNDSTMKGIGFFRMI
                     YFLPVVTSPIAAGMGFSFLFSTELGYVNHIIASLGGEYVNWLTNPEGIFQILLNWTGI
                     TLPKVLQGPSVSLFVIILMGIWQNIGYAMVVYLAGLQNIPTTYYEAASLSGASKFQML
                     RKITVPLLSPTTFFLLIMFSISSFQVFGPIMVMTPDGGPLNTTSVVVFRLYREAFSYY
                     RFGYAAAMAFVLFLFVISLTVFQVRTIERTVHYE"
     gene            121414..122235
                     /locus_tag="PKL93_01720"
     CDS             121414..122235
                     /locus_tag="PKL93_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70015.1"
                     /translation="MSKRVGRTVKYVILVVGAIVMVFPFFWSFMTSFKDLKEILRDPF
                     SLIPKEFTMKNYVSVFTKVPFARYLLNSTVVALATTFLQLLTASLAAFAFARMRFRGR
                     DVIFYVFLATMMIPQQVVMIPQYLVVMKLNMDNSYLGLILPHASTAISIFFLRQFFLT
                     IPRDLEDAATIDGCGPLRTLVNVFLPLTKPAIATIAVFSFMWSWNNYFWPLLVISEPK
                     MRTVQLGLAMFKSEGGIQWGEFMAATVVATLPIMIAYFIAQRQFVKGITLTGLKG"
     gene            122252..123496
                     /locus_tag="PKL93_01725"
     CDS             122252..123496
                     /locus_tag="PKL93_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70016.1"
                     /translation="MKRIITVFALLVFSLTGLGAVTLTFWHSMSDYQKPIIDKLVADF
                     NRSNDGIEVKAIFQGSYDETVTKLKAALLAGKGPDIAQVNIEHIQIFSKDGSLQDLTD
                     LISNDPTLSAEDFVESFWQTIIRDGKPYALPFNISALMLFYNKDHFEAAGLDPNKPPK
                     NWEELEEYARKLTIRKSSDSRPSQYGLLWGVDTMFYQFEPLVWQNGGEIFNEDMTECI
                     INSAEGIEALETWRRWFAEGLSPADLTIDEGIQSFTMGRISMGPMTSGGIRYALENIP
                     WDLGVAPLPEGKLKATTLGGGSLSIMSGLSEEKVKAAWTFIKWMVSEKNTLFWYEGTG
                     YMPVLKSAMDSLEIQLIWQRFPQLKAPIESVVFARPRPVHTNIIEIRNILYNAVEQVR
                     KGVSEPKPAFDEAVIKVNELLK"
     gene            123506..124696
                     /locus_tag="PKL93_01730"
     CDS             123506..124696
                     /locus_tag="PKL93_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70017.1"
                     /translation="MKRVLLATVLLLFSLTAFGADRIVGIEVTGVHKTNPRVVINISG
                     LQIGEELDIEKVYRAKRNLEDCGLFTDVYLQLNDVDGDYKLIISVQERNTIYPYFGEY
                     LALGVGDKSFLGSGVNLYGGIRFLRFTDEKLLGVLPKPQFFWGGLTLGASTFSAFGSG
                     LDPFLELTLFREVPWRDDLILTLDSFGAGTTYPFEDLVVGISYAYEKATFDATPLNNY
                     SLSILSGAFAFGDEENYEEKRGNFYGQTTLSYGFGKGINYLNQKASLTYWYRIIGRIY
                     VQSDTMAGLTYFGQAPVTKQFYLGGNLDLKGWEPYSFNPRFFLLEILEAGVPLTESFG
                     ISSSDEMSVFIPKLYLMGALGDNETLGMSVGIGFEWKTPLGVAVEPQLFFGGSGFKFY
                     LELN"
     gene            complement(124794..125456)
                     /locus_tag="PKL93_01735"
     CDS             complement(124794..125456)
                     /locus_tag="PKL93_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNQ70018.1"
                     /translation="MNFAGIESVSLVDYPGKVALTLFTSSCNFDCAYCHNSELKKRVQ
                     EELAESEVFDYLDKRGKLLDAVVITGGEPTLRANELLPFMRRLKSRFPETLLKIDTNG
                     WNAEILQEFLKIVDYVAIDLKSLHYEIFSPVNLREIEDSIEIAKGFANHEIRITMFPA
                     YLPEDDFEELSRICGGASLVSVQQYKPVAGLCETPPYPSSVLKRFADLISDHAQKVII
                     RE"
     gene            complement(125374..127527)
                     /locus_tag="PKL93_01740"
     CDS             complement(125374..127527)
                     /locus_tag="PKL93_01740"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNQ70019.1"
                     /translation="MLNVRKRNGAIVGFDLDKIINAMKKAFDATETHYNEDILERVAL
                     RVVADFQPKATDNLIDIEDIQDSVERTLESLGFTDVAKSYILYRRHREKLRELDSSML
                     DFSKTVNSYLDMLDWRVHENSTVTYSIGGLILHNSGTVTANYWLREIYDPQIGNAHRE
                     GDIHIHDLSMLSGYCAGWSLKQLIEEGLGGVTGKVSSSPAKHLSTLVNQMVNFLGILQ
                     NEWAGAQAFSSFDTYLAPFVKIDNLSLKEVKQNIQSFVFGVNTPSRWGTQAPFTNITL
                     DWVVPTKLRTQPAIVGGKALDFTYGDCQEEMRMINRAFLEVLDEGDANGRGFQYPIPT
                     YNITKDFDWEDEDAKLLFEITSKYGTPYFQNFINSDLDPDDVRSMCCRLQLDKRELKR
                     RGGGLFGSDEFTGSIGVVTINLPRIAYLSKNEEEFFARLSKMMDLSSRSLEIKRAVVE
                     KLNNEGLYPYTKRYLKNFDNHFSTIGLVGMNEASLNARWIKKAISEEEGYQFALRVLD
                     FMRSKMMDYQEATGHLYNLEATPAESTAYRLAKTDKEKFPQIITAGKENPYYTNSSHL
                     PVDYTRDIFSALDLQEELQQKYTGGTVFHSFLGERISDWETTKQLVRKIAENYKIPYF
                     TISPTYSICPDHGYIRGEHFTCPECGKPTEVYSRITGYYRPVQNWNTGKQEEFKTRET
                     YRVGRERVDEFRRNRERKPSGLSGESSPDTVYLKL"
     gene            complement(127749..128297)
                     /locus_tag="PKL93_01745"
     CDS             complement(127749..128297)
                     /locus_tag="PKL93_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNQ70020.1"
                     /translation="MEFVVIDTETTGMNPYRGAKLIEVAGVRVKNWELCRDDFFDSLI
                     DPECIIPIAITALTGISNLTVKGKSTVCDVLRGFYSFAGDATLIIHNAPFDLSFINFY
                     GDKCGLGKLRNQFIDTIEVSKAVFRYGRNNLDILLARLGIIPESRHRALGDALATAEA
                     FVAMLNRIGTNSVTRFIKKPNI"
     gene            128448..128768
                     /locus_tag="PKL93_01750"
     CDS             128448..128768
                     /locus_tag="PKL93_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70021.1"
                     /translation="MDDKVNHSEEEITERYRKALESAIQKAPIRSGERLYLTDLWAIT
                     SIPEEVIVETLGSSEVKLPEGVSCIVDDRRKKKRIIYGKREPNEKGSNPKQSRIQKVQ
                     GEDQ"
     gene            128689..129762
                     /locus_tag="PKL93_01755"
     CDS             128689..129762
                     /locus_tag="PKL93_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ70022.1"
                     /translation="MGNGSQTKRVAILSSPEYKKCREKISEGLDLIGFDETLRGKKVL
                     LKVNLLSARSPESCVTTNPLFVRAVAELFLGRGAEVSVGDSPASVSTSVAGYASGISQ
                     VCKELGIPLLDFDDPVQIVFDEGTYRSFLISKPVLETDLLINLPKLKTHALTRVTLGV
                     KNLFGCVPGAKKQGWHFRVRNMKEFSKMLLDLAVHLRADLTVIDGIYGMDGNGPSNGR
                     IIKPGIIGMSRDVFALDDAIAELFGINHSKVPILQIARDRGLVGDYDVVGDEVHPDKL
                     LLPETNFIGVYGAAILRRIVTKFPKIDRERCRSCRVCENACPAKAIDINRFAIDYNKC
                     ITCYVCHELCPEDAIVFTRRLFK"
     gene            129773..131233
                     /gene="glpK"
                     /locus_tag="PKL93_01760"
     CDS             129773..131233
                     /gene="glpK"
                     /locus_tag="PKL93_01760"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNQ70023.1"
                     /translation="MNYVLAIDQGTSSSKAVLFDQELYQVAVAQEEFKQIYPKPGWVE
                     HDPRDIILSTMSTIERVVSDARISFREIAAIGITNQRETVVAWDALTDQPLYNAIVWQ
                     DRRTAGRCKEIESSYGELIRRKTGLKPDPYFSATKMEWLLENVPAVRDAANRGRLRFG
                     TIDSWLVWNLTRENLHVCDFSNASRTMLFNIEELKWDNDLLNLFGVKEEYLPTVVESC
                     KLLGPSIYGPRIGGMAGDQQASLFGQTAFQTGDAKCTYGTGSFILMNTGKKPKISEHG
                     LLTTIGWKSKEEIVYAVEGSIFATGALISWLKDGLGIIDAPSETESLAREVADNGGVF
                     FSGALTGLGAPYWNSSARGMMIGLTRGTRKAHIVRAVLEYIAFRTREILDLMEEETGI
                     RLNKLLVDGGVTRNNLLMEMQANVLNIPVDRSLIKETTALGAAMLAGICTGLWDLESL
                     KNKRISEVVFEPSGDRMEEEFRRWKEALKRSMNWAN"
     gene            131191..131748
                     /gene="tsaE"
                     /locus_tag="PKL93_01765"
     CDS             131191..131748
                     /gene="tsaE"
                     /locus_tag="PKL93_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNQ70024.1"
                     /translation="MERGAEEIYELGELDLREFERVAKKIGEMLEGGETVLLFGDLGS
                     GKTTFVKSMADGLGIDRDYVRSPTFNIVNSYPRRSSRKENNENPVQVERPGLIHVDLY
                     RVDSEEEMLDLALHDLVDLDTVVAVEWPELYVKYVSQPYLIVRLEHCDESKRSMRIEP
                     HGRKMTTLLNRLVMIINEDERREGE"
     gene            131749..134124
                     /gene="lon"
                     /locus_tag="PKL93_01770"
     CDS             131749..134124
                     /gene="lon"
                     /locus_tag="PKL93_01770"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNQ70025.1"
                     /translation="MAQKFELLEKAAVGSERKIEIPEKLPVIPTRTNMLVYPSAVMPL
                     YVGREKSLAALEESIGKFNQMVFLVSQRDITKEDPEIEELFEIGTIARIVQLMKMPDG
                     NYKILVEGLTRAKLVSVEEKENSLIVVVEKLKGKGRKSKMLQALVRKVKELALRYVSM
                     SRRFPDEAIMALEDTSDADKFGDFVSSMMPFSLEEKQRLLEEIEAKDRLNTLMELLTR
                     EIEILSLEEELDKRVKEKIEQGQKEYYLREKMRAIQEELEGEEDEEIKELKNKAESGE
                     LPQEVKEKAEQEISRLEKMSPYSPEATVVRTYLDWLLNLPWQEETDDEIVIRDVRKTL
                     DSNHYGLEDVKERILEFLAARRFSKNLRAPILCLVGPPGVGKTSLGRSVAEAMGRKFG
                     RISLGGMRDEAEIRGHRRTYVGALPGRIMQMIRKLKTRNPVIVLDEVDKMGISFQGDP
                     ASALLEVLDPEQNNNFTDHFLEVPFDLSRVLFITTANVLYSIPAALKDRMEIIEIPGY
                     TESEKYHIARDHILPKLLEEYNMDSSKFRITPAAVRDVIRDYTREAGVRELDRNLAKI
                     IRKATLKIAEGSDSISVGVNELGEFLGAPLFKESDFRKHPEVGVVTGLAWTSVGGEIM
                     YIEVLPVAGKGKTIITGQLGDVMKESAQIAASLARKLCDDERFSEVFEKKDFHIHVPE
                     GAVPKDGPSAGITLTTAIVSAVTGRRVRNDIAMTGEITLRGKVLPIGGLKEKLMAAYR
                     ARMKKVLIPLANKRDLEKVPDEIKSKLEFVFVEDIREVLKEALIPGDGKEH"
     gene            134105..134677
                     /gene="yihA"
                     /locus_tag="PKL93_01775"
     CDS             134105..134677
                     /gene="yihA"
                     /locus_tag="PKL93_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNQ70026.1"
                     /translation="MTVKSTELVRTVFSKGEYPPPLRREIAFAGRSNVGKSSLLNSLF
                     GRKLAKTSSTPGKTRSINFYGVNREIYFVDLPGYGYAKASMTEREKWKKLIIDYFESR
                     EELSLVVVLVDIRHPLQPADIQMLEWVTYYAIPFILVLTKADKLSRSQQIIAEKSIKA
                     ELASLGVYPPVFVVSAVKKQGIEKLLIALL"
     gene            complement(134734..135081)
                     /locus_tag="PKL93_01780"
                     /pseudo
     CDS             complement(134734..135081)
                     /locus_tag="PKL93_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
     gene            complement(135166..136407)
                     /locus_tag="PKL93_01785"
     CDS             complement(135166..136407)
                     /locus_tag="PKL93_01785"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HNQ70027.1"
                     /translation="MLSFPDGFMWGVATAGHQIEGGNVFSDWYAWEHQGKVKNGDTSD
                     VACGSWEHLERDLEAIKALGVNAYRFSVEWARIEPKVNRFEDSVIERYKDFVTMLIEN
                     GIQPILTLNHFVLPQWFSEIGGWEDRENLPYFRRFVSTIVSSMGENVHYWVTINEPNV
                     YAVMSYLMGEWPPEIKDMGRAMRVLANLLYAHSEAYDVIKESNPLSMVGVAVNMIPFF
                     PLRTFHPGDRIVSKYLDRVYNYSFLDSLKNGKMVKPLGTGEAVSGISSKLDYLGVNYY
                     TRMFAKYAKPLPEIVVGDEFEKTEMGYEFFPQGIEDVVLKAYKRYELPIIITENGIAD
                     GTDKRRWEYIETALKSLRSAMDKGARVFGYIYWSLMDNFEWKEGYSMKFGLYETAREN
                     LELRPRGSADKFRDFIRESLS"
     gene            136506..137354
                     /locus_tag="PKL93_01790"
     CDS             136506..137354
                     /locus_tag="PKL93_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ70028.1"
                     /translation="MKISIVGNVNIDLSAFISESGNNEENRISEMSFAIGGSASNTSI
                     MLSRLRKSIYLQGAVGSDFFGEMALELLKREGLNIDYLLKLNGKTGFCFSAISKDGQR
                     HLFTYRGVNEKAYPIEDGFDFYHFGGVTPDQIERFVGKTGEVRFSYNPGGIVTFEKTN
                     GVAAIAARCEVLFVNESEWNHLEHSLTVEPKEIVVTLGARGSKIKNGPEEAALPAKVI
                     DTTGAGDSFIAGFLHAYLSGKDPAQCLKLGNLLASMVVSRPGASPAFTYNEVSDLANR
                     YDVYLH"
     gene            137534..138475
                     /locus_tag="PKL93_01795"
     CDS             137534..138475
                     /locus_tag="PKL93_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70029.1"
                     /translation="MPAQIASVDVVRILGKLSDDGGEQSSRNRFIREYLSKYTSPESI
                     VSDIEGLLEAYPTFDGDSESLGRAYADLVNRLAELSGFSVDYVKYSGREKITGIWKVG
                     EAIQIKVSAIICTDFEEAKGVARNALLILPEAIDLGRPFVTVGRLGMLFSMIEQIELP
                     VSSVASILVTDDNYDKRISSFMELMLLAFKKELARKPQISLQKEWSKEELEEFILERL
                     KLPTIGMLFSLLDSPMLSEDLVDRINSFLEENESELVKGPMALGAIMGALSKHYSGIK
                     EELVLREGKRYRLADKYRPVISEIKARFESGVQARTR"
     gene            138689..139588
                     /locus_tag="PKL93_01800"
     CDS             138689..139588
                     /locus_tag="PKL93_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNQ70030.1"
                     /translation="MDFSESVERFKENMEFVRNMSSNTISAYLSDLHHFELFLNERGI
                     DYKSVKRKDIELFVKEYSQGKYSRKRPSATTVARNLSTIRSFYTFLYISGTVGKVPTE
                     LIKNPKTRRRIPDYITHEEVMEILSSFRDTNLGKRNKAIVATMYFCGLRVSEVCKLRL
                     GDLRMGNSPAVRIMSGKGNRDREVPMNDQVLSIMKDYLEIRREFPVSEYEDHVFIGTR
                     GEPMARNVIPKVLNTQVKYVYPEKRVHPHLFRHSFATQLLQKGASIKTVQELLGHANL
                     STTSIYLHITDKEKRAAVQLLSD"
     gene            139618..140370
                     /locus_tag="PKL93_01805"
     CDS             139618..140370
                     /locus_tag="PKL93_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNQ70031.1"
                     /translation="MIDIHNHTTYSDGRNTVEEVIQSALKEGLQIVGISDHFDPYVAQ
                     EVCLQPEEVGVYRKEIRTLSEKYPVKVLAGLELGLQTEGILWPGEEMDYYIYSVHTVP
                     GRPDLKTLENPWDIYLQEAISAVDLIDRPGFLGHLDFLRRHIPKAKPPKPGQLMDTLL
                     MKLVSNHVGLELNTSGWMYGIGDPSPQPWVIERYIDLGGRFVTVGSDSHRASQIGNFT
                     VKALSLLRKMGIKEVFYCENGSYIPVPIMEAK"
     gene            140367..141059
                     /locus_tag="PKL93_01810"
     CDS             140367..141059
                     /locus_tag="PKL93_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNQ70032.1"
                     /translation="MSEKSFEGFSGETLSFLIDLGINNNRSWMERNRDRYRIELLEPF
                     RALVKQLGPSMERIDPFIDVRPQVGKTISRISRDARRNKGKPPYRTNMWFTFRVPGPD
                     WKDSPGFFFELFPDWYRYGMGFYLPSRATMVKLSEEIEKNPVRFVEKTKVLRDREFSV
                     FGEKYKRRRNRKGVSDSLSEWLQYKDFYIASNHEIDEILLSSRLVGHLEAAFSALSEL
                     YEYFWELKMRDA"
     gene            complement(141082..142239)
                     /gene="wecB"
                     /locus_tag="PKL93_01815"
     CDS             complement(141082..142239)
                     /gene="wecB"
                     /locus_tag="PKL93_01815"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNQ70033.1"
                     /translation="MAFANGKKEGTASRLKKMKKVALIFGTRPEAVKMAPVFLALKNS
                     PIRPVVIATAQHREMLDQVLSLFGIEADFDLNIMQERQSLAGLTSRLISKLEEIYSEN
                     VFDATLVQGDTTSTFAGALVSFYHKIPVGHVEAGLRTENVYDPFPEEINRRLNGVISS
                     FHYPPTENAKNNLLKEGVPENKLLVTGNTVIDALLWVTKNRSRLTSSFREALGVEEDE
                     YILVTMHRRENWGEPMRQVMKAIKDILKEFPKMKVVFPVHLNPAVREVVYPELEKHSR
                     IVLTDPLDYLPFVSLMEKARIILTDSGGIQEEAPALGKPTLVLRKTTERPEAIKAGTA
                     KLVGTDRDFVFKETVKLLRDSDEYRRMANAKNPFGDGTASKRILKHLCENL"
     gene            142345..143862
                     /gene="murJ"
                     /locus_tag="PKL93_01820"
     CDS             142345..143862
                     /gene="murJ"
                     /locus_tag="PKL93_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNQ70034.1"
                     /translation="MTASTVRSTAIFAIATMLSRLTGLARDSLFANYFGTSAQYDAYL
                     VAIMIPFFLRKIFADGALTMAFVPVFNEKLKISRERAFIFASTVIVFVVIVAGSISAG
                     GMVFSEGVASVFAGGFDKDALDLTSRLIRISFPFIALVSLWAVYCGVLNSLDAFFIAA
                     VSPMFINLSTIAGILFSERFSPPIVGPTIGFLAGGVIQLVVVALAAKSKGFVFKPGYS
                     KSDVKEFLILFLFSAVSPAINEINSFVDVRVSTELGRGAVSSLGYAQRLYQLPLGVFA
                     VAVATVALPRLSKLSGSDARDRFRKALWDSLTVLAFLIIPSTLGLLVLGEGIVRILFE
                     RGSFTPSDTALTTSLLYGYTLGLPFYGSYGVLSRAYYARKSPRTPTIISAIMVSVNVA
                     LDILLGFTIGPLGVALATSAAGIVGTVTVSVALFRWSGYDREKIIAILKIILASLVMS
                     CVMIVGRTLFGTGFISTSLTLVAGIIVYFLFARILRVGNLFRLRDLVRNKRSSSQ"
     gene            complement(143856..144608)
                     /locus_tag="PKL93_01825"
     CDS             complement(143856..144608)
                     /locus_tag="PKL93_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNQ70035.1"
                     /translation="MSIDLHNHSSFSPDSKTPVEEIVEEAILKGLKVIGISDHDDLDD
                     SFPDSYRMKDPEIYLKSLSRMKENSIVKVLAGLEVGLQSCAKTLPEGDFDYFIYSVHG
                     IPGIKDLTSGDVWTLYLEEAIEAISGIDRPGFFGHIDFLRRYLPGHKALDNEELLCEL
                     LRKLIRAGIGIEINTSGWRYPYKEPSPQRWIVEKYFLCGGKFVTIGSDSHRREDVGSH
                     VSDAVLLLKSIGFKEVFYCEKMEYKPVALTIY"
     gene            complement(144612..144923)
                     /locus_tag="PKL93_01830"
     CDS             complement(144612..144923)
                     /locus_tag="PKL93_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HNQ70036.1"
                     /translation="MIHLKELSVIIEHTLGIEEAIKRLNKKIEELKSEYEGKFALDST
                     WEDDSLRFRLKAAGMKTEGSVKFADSTVTVSGKLPLTLAAFRGRIESTLRAELLKVLC
                     D"
     gene            145000..145557
                     /locus_tag="PKL93_01835"
     CDS             145000..145557
                     /locus_tag="PKL93_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNQ70037.1"
                     /translation="MKKIYLVIDTETTGSSPLEGDRILEIAAIPVYGNKILHNLSFQS
                     LVNPLVMIPAQITGIHGLKNEDVLDQPTMAEVFPRFRNYVGGATIVGHNIVNDMTFFD
                     IASKETGILPLANNYIDTIDIAHEVFSEGPYGLKSIAKRLRIRDVPTHRAMDDARVTA
                     KVFLALARRLGGISQMVKYEKKWRG"
     gene            145560..146846
                     /locus_tag="PKL93_01840"
     CDS             145560..146846
                     /locus_tag="PKL93_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNQ70038.1"
                     /translation="MIKDFILDTNVLVHDPYCFDNFEDNNLIIPFPVLEEIDKLKKNS
                     GSVGQNARKVNRFLDSLRSKGRLTEGVRLESGGTLRIAVFNEFKSQLPPFAASGYKDN
                     AILLYMKELELVDKLPVILVSKDINMRVKADIMGMKAEDYLHDKVEIDDKLSGVNTFD
                     DSALRDKFIDRDELSVDELSDSVGANEFVDFGKGIFGRVTPDGEMIVPLEVKMDTSSW
                     GIKPRNYEQMMAMELLLDDRVRVVFIPGIAGTGKTLISLACGLRKVVDEKKYERLLVA
                     RPIIPMGQDIGYLPGSMEEKIDPWMTPIYDNLYLLFNNRHMDMDSFLKKREQLEVEVL
                     SYIRGRSIPNQYFIIDEAQNLSPHEIKTIITRVGENTKIVVIGDPYQIDNSYLDAYSN
                     GLTYAASRFRDKELAGHITLMKGERSELASLAAELL"
     gene            146856..147098
                     /locus_tag="PKL93_01845"
     CDS             146856..147098
                     /locus_tag="PKL93_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HNQ70039.1"
                     /translation="MGEVLEYDLFVRIRPEDIHVLCYIAEAEDNLMNIRHVTDEGLLK
                     IIVPTDLLEEVKSFLQSIKNRIELEVVEIRANPGHT"
     gene            147076..148128
                     /locus_tag="PKL93_01850"
     CDS             147076..148128
                     /locus_tag="PKL93_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HNQ70040.1"
                     /translation="MRILDILDNAVPNEKAENVREIDYERLRKLGYNTILFDYDNTIA
                     VWREPFDMRNKPVIDSLISSGMKVGVVTNGPQSRVKNLKDLFGEDLKVYHSMRKPGTK
                     ELRKVLSEMKSRPEKTVIIGDLFFTDIIAGNRMGMYSILVSPLVDISHKWYKRLLGKV
                     TIAVYLVFFFTIGWIFRTGRLATPHLFATTVMDIDFDSLKDSGYRLIIFDFDNTLEKW
                     GATSVSKEKRLLISRVERLGLEVVLISNGKSNRLGKIERELRTTKVISRARKPFTFKA
                     KRVLKDFGIPPYKTVIVGDQLFTDIIMGNLLGAYTVKVEPISDREFFWTKLVRRVESL
                     ILSKMRKHPEVEAMDR"
     gene            148125..149231
                     /locus_tag="PKL93_01855"
     CDS             148125..149231
                     /locus_tag="PKL93_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNQ70041.1"
                     /translation="MKCGGCGVELQHDDPAGLGYISRSVMESRLQSGKQVLCRRCFLI
                     KHYGSLSEGDRVVHSIDNLKEYLDLAQDVIYVIDISDFDGTCRRDILRLLKERTVHYI
                     LNKIDLLPKEVKIDEIRDWAASMLKVPKETVRPVSVLKNYGLKSLFSYLKSASEEFVS
                     IGVTNVGKSSVLNGLIQSEELTVSRFPGTTVKVASKKVGGSAFTVYDTPGIFTKDRIT
                     DLLTVEDQSRFLPRKKLCRSTIQFHDVRTVFLGGFVRLDVKNESDPVGIFHVFVPESV
                     TVHETNSDTGNEEWDRWYGGILKPPFSESIRSNYKWKSERFRLRTGQELHIFGMGWIN
                     VAKGPLTITVTTPEGVATKVRKGLIGAKKFKKQK"
     gene            149246..149839
                     /locus_tag="PKL93_01860"
     CDS             149246..149839
                     /locus_tag="PKL93_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HNQ70042.1"
                     /translation="MKLKQPAIKTGVSNRHLHLSAADIESLFGKGHELTPVKDLGQPG
                     QFACDEKVILVGPKGAITGVRVLGPARKATQIEVSRTDSFTLGIKPPIKDSGDHTDTP
                     GLTIVGPKGTVVLNSGVMLAKRHIHMTPADAEIFGVEDKDIVMVYAEGAGTRKVVFDD
                     VLVRVHSSYALEFHVDVDEANAAVLNNDDPVYIVEEL"
     gene            149840..150634
                     /locus_tag="PKL93_01865"
     CDS             149840..150634
                     /locus_tag="PKL93_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNQ70043.1"
                     /translation="MIYLGTSGYSFPDWVGTAYPAEISSSQMLRYYHSVWRFNSVELN
                     FTYYRMPTYKTVTGMIRKIPGEMIFAVKAPGRVTHEIWKNGVNDLQEISDEFYNALFP
                     MKEEGRLGPLLFQFPWSFKNNKENMSYIKAVAKTFSSDKNLLAFEFRHESWAADEVFE
                     TIDRLGVVPVTVDEPSFGGLFPYLPRSGSRGAYFRFHGRNPDWFNSKGSERYNYDYPK
                     TDLRRFALDVLEFHKHDLPVYVFFNNCYMGRAVHNALLMRDILGGA"
     gene            150642..150971
                     /locus_tag="PKL93_01870"
     CDS             150642..150971
                     /locus_tag="PKL93_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70044.1"
                     /translation="MTFSDYINVVLRMIAEGNESHEIYEQLQKLGLREVEITEVYNAV
                     ISMITSGSESTEVILDGYYDPRKRVFLDEELKAIISVPGLYKSLLWGDISPGDFERTL
                     SDFSSYS"
     gene            complement(150998..153565)
                     /gene="glgP"
                     /locus_tag="PKL93_01875"
     CDS             complement(150998..153565)
                     /gene="glgP"
                     /locus_tag="PKL93_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNQ70045.1"
                     /translation="MYFLERVMAVPRLPEKLIRLEELSKNLWWSWNYRAQQMFKYMDP
                     EMWYQERRSPVRLLRRIRQTRLYELSEDKPFIEMYESVVGEFDRYMGGKDTWMDREHP
                     DRKEQCIAYFCAEYGFHESFPIYSGGLGILAGDHLKTASDMGLNFVAVGLLYRNGYFE
                     QRIDESGWQQNIYHSYEFEDFPVVPAQDDSGDEIFVNIDFPGRKVWAKIWKAAVGRTS
                     LYLLDTDIPQNEPEDRNITATLYGGDRETRIKQEILLGIGGVRALRMLNYSPNVWHMN
                     EGHAAFLSLERIRELVQKHSIDLSTASMIVKSGNVFTTHTPVPAGNDTFSLEIIDKYF
                     RDFWSTIRAERGEFINLGIEKLPGGGQQYSMTVLALRLSALANGVSKLHGRVSRNLWN
                     HIYPDLPAVEVPISHVTNGVHIWTWLNPNLVKVLDKYLPVGWHEKVYDPEVWEAVDKI
                     PEEEIWELHMSLKSRARNFLQGRLKRQRMRLGETIEDLMEVEEALPEDVLTIGFARRF
                     ATYKRATLIFKDINRLKSIITNSERPVQFVFAGKAHPADDPGKELIRKLYEISRTPEF
                     KSRIIIVENYDMNIARHLVSGVDIWLNTPRRPHEASGTSGEKAGMNGVLNFSVLDGWW
                     VEGFNGVNGWAIGDNRDYKDHELQDRIDSVSIYSSLEKEIIPLYYSRDEKGVAKEWAK
                     RMKNSIKEIGSKFNTHRMIADYAEDLYFPVADLSKEVEKDDFKLAKSVAIWKDKFSSS
                     WNAIRIKPNMIGETSAKSVKVGQEISVSANIYLGTMDPSEVQVEIFVAKMNDEGEMDG
                     FSLYPMKLVKEDVHGEYVYGGKFTVAEEGQLAYTVRVIPNPERLPHRYFIPVAKWIT"
     gene            complement(153727..154095)
                     /locus_tag="PKL93_01880"
     CDS             complement(153727..154095)
                     /locus_tag="PKL93_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNQ70046.1"
                     /translation="MSVKVGTVVEGNVTSVKKFGAYVNLEGGEEGFIHISKVAREYVK
                     NIEDYLKVGQKVEAKVLGTTKDGKWELSIKDLKGKGSEEAPTSQDFEKKLAKFMRDSG
                     EKISAYKRRLDKKRGIRKRP"
     gene            complement(154146..154355)
                     /gene="rpmE"
                     /locus_tag="PKL93_01885"
     CDS             complement(154146..154355)
                     /gene="rpmE"
                     /locus_tag="PKL93_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNQ70047.1"
                     /translation="MKKEMHPEMKLVTVKCTCGAEHKFYSTKENIKIDLCSSCHPFFR
                     GDASSMILDTEGRVQKFRNKYGDNY"
     gene            154469..>154896
                     /locus_tag="PKL93_01890"
     CDS             154469..>154896
                     /locus_tag="PKL93_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HNQ70048.1"
                     /translation="MIDDILNFVKEAASSEGFYAFYSNRKELVERLNRALKRSPVSSA
                     TVGKLRKRIAGLAHLSYEEIEFSIDILRERDRAPEERVEYASTLFKVSVSDMGHLLFF
                     VDPRNNPPVTGPVKERIQSIDDYKEWQSLTRSVGEHGIQD"
     assembly_gap    154897..154996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <154997..155625
                     /locus_tag="PKL93_01895"
     CDS             <154997..155625
                     /locus_tag="PKL93_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HNQ70049.1"
                     /translation="KEWQSLTRSVGEHGIQDFVMLEAALLFEREENNRSSNLSERLIR
                     AVYTDISEIKTLRNAVTSLSKSEKRLLSNLRFTHPYVKTAIMSNHSKPVVIDGSNIVF
                     SMTEHADLKRIDDLFLRMALCRIALFPYRIVFDANIRYTIGGFQQESLNRLLSLPQVE
                     TYSPADDRLIFLAREGGSAVITYDRFLDHAAEDITIIRPEEIDESLRL"
     gene            155606..156616
                     /gene="queA"
                     /locus_tag="PKL93_01900"
     CDS             155606..156616
                     /gene="queA"
                     /locus_tag="PKL93_01900"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNQ70050.1"
                     /translation="MKVSDYDFELPEELIAQKPAIPRDSSRLLVIDRETYSFEHRVFA
                     EIENFLHPGDVLVLNNSRVIPARLLGKKETGAKVEVVLTEKLDGGLWKAIVRPGSKIK
                     LGNVLLFNKISCKVIEHCDDSSRILDFGSVTDEDVKKAGLLAIPPYVKYYPDDPESYQ
                     TVYSRLEGSVAAPTAGLHFTDTLLERLRKKDVDVQFITLHVGIGTFRPVKSETIDGHK
                     MHNEFFSVSSEVAERLERARKEKRRIVAVGTTTVRTLESIIARHGSIVGDSGHTDIFI
                     YPPYTFKAIDSLITNFHLPRSTLLMLVSAFAGRELILSAYREAIIKKYRFFSFGDACL
                     IL"
     gene            complement(156699..158135)
                     /locus_tag="PKL93_01905"
     CDS             complement(156699..158135)
                     /locus_tag="PKL93_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNQ70051.1"
                     /translation="MSREILKKPRFNAAIFTLFYLALFFMVFSVSLQTAMILFATFTV
                     VIILEPWARFAERSFHIKRSISLGIGLAVIFSGLTLLIVFLTTPVAKEASKFYNIVRD
                     FFPSAEETEITSFEVNANIDRFLSEEEFLSKLSPEEVESLDLLSKDLKRYYSEIMDPI
                     TANSEDLNEELALLEETLKNRSDYVVIISSGEQKNASYEEIHKIISNYLNPEAAELLS
                     ASVLDNAMVVHSQELWRNVVDYFMPKNVDPSTQRATYESVRNFLIQVQSSLMKFLPGI
                     LEKIPSFLTSTGLVLFFTIVGAIYLSYYFITVKDFIPRFYPKKVRGIAMSFLEDTYKN
                     LERYVISVVVIAMIVGVVVGIVVHSMGLKYSLLMGMWAAFTNLIPIVGVPLEFFPLFL
                     LAVSTQDLVLVLVLMIILVVVHASAFILFLVFMKGYNRINPVIIILMIVIAGQLWGLF
                     GAIIAVPFAIILKMTWMHFFSPMLEEEQ"
     gene            complement(158137..159132)
                     /locus_tag="PKL93_01910"
     CDS             complement(158137..159132)
                     /locus_tag="PKL93_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNQ70052.1"
                     /translation="MKQRVVIVTTGGTIAMITDPATGASVPAQGLNKHVADVDGLKDV
                     ACTEHYEFSNIPSPYINPETMWALSKVVQKLLDREEVCGVVITHGTDTLEETAYFLDL
                     TVRSEKPVICTAAMRNIDEMGTDGPRNVLSSVRVAVSPLAIGMGTMVCLNDEIHAARE
                     VTKTYTSNVATFDSPGHGPLGIVDEDTVIFFRKPLLRAVFNVEKIESRIALVKTFTGD
                     DGSILRILPDLGFKGVVLETFGRGNVPVEVYHVVKYLIEEKGVPVIITSRCFKGRVLG
                     VYGYVGGGKSLADIGAIFAQELSPIKARIKLIVLMGITKEIPEIERLFKLPLKGA"
     gene            complement(159129..160769)
                     /locus_tag="PKL93_01915"
     CDS             complement(159129..160769)
                     /locus_tag="PKL93_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ70053.1"
                     /translation="MNALIIDGYTDEPAAFGVPPYISPRIRAIAGAFLLKGIETDYLT
                     IDSVRQEGLWEKGNDYDFLIVFGGVTTSGRYIGGTPISSSEISLLLKSNSDPTKIVSG
                     PVVSVGYTLRGGTTAFTPDFGEADYLLRDEFEIADFLGLDSGGSRYSLLNRLYPAGAD
                     VIRLHPCFPDLMVEIDISSGCERLDGFCSFCTESIFYGSFEWRSIKGIVAEFEKLKEV
                     GVKAIRFGRSANVIAYGYDRRRDTLDPSLSEELFKSARSIIQPEVFHIDNGNPIFIAH
                     HPSESRSVIESIVRYNTAGDTISFGVESFDDEVRSLNNLGGSVQDIIFSIELVNEIGK
                     ERVEGVPKLLPGINLLYGLPGQSRKSFDIDYDELVSIQERGLLLRRINIRQTMIFPGA
                     RIFNMTRPRIDHRLFEKHKHRIRKEIDSKMLKKTFPLGAIIRGVIPEFSEGMIYFGRP
                     LGTYPILVGSPVGFEEITDFVVIDHGMRSITGLPVGTELNDLGEREMRFIPGIGRDRA
                     KSLMKKRPRTREELIKIVGPETHKTLSLIKTKLGGKWL"
     gene            160841..162052
                     /gene="clpX"
                     /locus_tag="PKL93_01920"
     CDS             160841..162052
                     /gene="clpX"
                     /locus_tag="PKL93_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNQ70054.1"
                     /translation="MPFERHCSFCGRSAAQVKKLISGTEGYICNECVDLFHDIIQQDQ
                     KAKKEPGKKLPTPADIKKELDKYVIGQENAKKTISVSVYNHYKRVFNDVDDVEIEKSN
                     IMLVGPTGSGKTLIARVLARILDVPFAIADATPLTEAGYVGEDVENIVLRLLQSANYD
                     VEKAQLGIIYIDEIDKIARKSPNPSITRDVSGEGVQQALLKIVEGTTANVPPQGGRKH
                     PYQEFIKVDTSNILFIVGGAFDGLDSVIKQRVLDSSMGFGAKVKGKNQLRLGEVLQQV
                     VPDDLIQYGLIPEFVGRFPVLAALNDLDVEDLKRIMSEPRNAILKQYQRLLGLDGVEL
                     EFSDDAMTAIAERAMKRGTGARALKSVIEQVMLDIMFDIPTMNNVEKVVINEDVISKN
                     SSPEIISRESA"
     gene            162052..163044
                     /gene="tsaD"
                     /locus_tag="PKL93_01925"
     CDS             162052..163044
                     /gene="tsaD"
                     /locus_tag="PKL93_01925"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNQ70055.1"
                     /translation="MKVLALESSCDETAVAIIEDGLLLSSQISSQIDIHKRFGGVVPE
                     IAARKHLEIIDRMVSRSLEEADLDIKEIDVFASTMGPGLVGSLLIGLSFAKGMAIAMN
                     KPFVGVNHLFGHVYANFLKYPLLKPPFMVLLVSGGHTEILVLKDLDRIELVGKTRDDA
                     AGEAFDKIARLLGLGYPGGPAIQEAAEKGQAKYPFPRPLREKGNFDFSFSGLKTSVLY
                     FMKKNPLASVYDVAASAQEAIVDSLVTKVFEAAESLHLHEIAFAGGVASNSLLRERAR
                     VQSEVSGIKAYFPPVDLCTDNAAMIALVAYEKAKRGLFSPLSTNAVPYLTLDFF"
     gene            complement(163045..163278)
                     /locus_tag="PKL93_01930"
     CDS             complement(163045..163278)
                     /locus_tag="PKL93_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ70056.1"
                     /translation="MPFYRYQCEDCGEEIEAFQKMSDEPLTICPACGGHLKKLVNNVG
                     VVFKGGGYYSTDSKKPSASKSSSKPSTSTSKSE"
     gene            complement(163325..163687)
                     /locus_tag="PKL93_01935"
     CDS             complement(163325..163687)
                     /locus_tag="PKL93_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ70057.1"
                     /translation="MSERILVGKALDVEPQFFDNENVKSVTKRVLIGKKKGATNFVMR
                     LFTVGEGGYSPHHSHPWEHEVFIISGEAAIVTDQGVINAGKGSYVFVPSGVKHQFRNT
                     GNGDLEFLCVIPSSADEE"
     gene            complement(163653..164360)
                     /gene="jag"
                     /locus_tag="PKL93_01940"
     CDS             complement(163653..164360)
                     /gene="jag"
                     /locus_tag="PKL93_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNQ70058.1"
                     /translation="MNPVEYRAETVEEAIRLAQADLGAYDDEMQVTVKEKGSKGFLGI
                     FGSRESVIEVSLLDKHWERKLHDFLKGILKNFDEETFSEVKLVGRTYRVRIEGEEVAR
                     LIGKHGKTVAALQHILNIYANRMAPTKVNVTVDIGDYRLERKKLVEEIAHRTAKSAIS
                     RRSKIVMEPMFAFERRLVHEVISKYEDLKSYSVGLEPYRKVVVEYRDRKYSCDKAFDS
                     KRRRYENVRTNSGRQSA"
     gene            complement(164392..165738)
                     /locus_tag="PKL93_01945"
     CDS             complement(164392..165738)
                     /locus_tag="PKL93_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNQ70059.1"
                     /translation="MKRAIILTLIFVTLFTLSVSGKELTTQEGTNTLIVSGKTLKVTL
                     DTEKGLVLGVTSYADNKTVEFYVYNESLGKDGFNVFDVEGTELLPTSFSYDKDEEGNV
                     IVSFNYEQGSKSFIILNSPYYDFRVELNFNQKIEVALPFISYQNQTIGPDSSYYLTYD
                     KFSGQKTLFAITSMQGTFKFQRYESETGRGILESFAGPMRLIHVRQALPEHYDAIVKD
                     LQDFGGVTFFSWIYHGIVYFLFWLYQWSGNFGWALILFTIVTRFILYPLYHIQTKSML
                     MMRMLQPEVEKIKKKYKDPRKQQEAMMALYKEKGYNPASGCLPILLQLPVLILLYNAI
                     RYFSEVMAYTPGFLIWKDLSVGNFVLNIPFIIITVVVGIFSSLLTAQDARSARSGVIM
                     QVIFPILFATFPSGLLLYWTTQSVLQFFISWVVYKRHGIKGLTLRQMLGLQEKPVK"
     gene            complement(165735..165977)
                     /gene="yidD"
                     /locus_tag="PKL93_01950"
     CDS             complement(165735..165977)
                     /gene="yidD"
                     /locus_tag="PKL93_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNQ70060.1"
                     /translation="MKKILLFLIDFYRKKISPRTRPRCRFSPTCSTYTYEAIERFGAL
                     KGLILGIWRIIRCNPFNPGGFDEVPDHFTLLRRHEK"
     gene            complement(165922..166350)
                     /gene="rnpA"
                     /locus_tag="PKL93_01955"
     CDS             complement(165922..166350)
                     /gene="rnpA"
                     /locus_tag="PKL93_01955"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNQ70061.1"
                     /translation="MNLRDQSLSKTERLRRKADFDRVFKKGKSIVDPFFVVLYVKNGL
                     PFSRIGVSIKRKFGKAHLRNRLRRLVKEVYRTNKQVFPEGYDILFIARKDLSDLFKKR
                     KVGYYDIVQITKRIAEKIGETSDEKDTTVSDRFLQKKDLP"
     gene            complement(166347..166481)
                     /gene="rpmH"
                     /locus_tag="PKL93_01960"
     CDS             complement(166347..166481)
                     /gene="rpmH"
                     /locus_tag="PKL93_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNQ70062.1"
                     /translation="MKRTYQPSRVKRNRTHGFLVRSRTVGGRRVLASRRRKGRKRLSV
                     "
     gene            166642..168267
                     /locus_tag="PKL93_01965"
     CDS             166642..168267
                     /locus_tag="PKL93_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ70063.1"
                     /translation="MKATFLLLMEGDMLLSLAGKDFLTFKDFYVEFSGGMNAITGESG
                     AGKTVFLKALWAVLGFPPLWDNDGSGSVEGNFAVDDSLLSKLGDMGVDINGDQLLVSV
                     SFTGQRTIYRINGKMVPKQIVQSAFRDRVEIHSQHSSISLLDESKHHIILDHALGGDT
                     SLTRYSELYAEYIKVRRELDSFIVDPSQIEREKNFLEFQIREIEQAELQPHEDEVIEM
                     KYTRYRNAQMLLETFQELTDLLKDGELSIYNSLNDARATVEKIRNFGYKDWLDNLQIA
                     LEELDSLYTKIDEERSTLEIDDEEFSAIESRITIIQGLKRKYGDSIEKILVRLEEFKK
                     ELGTLKELEGRKERLKKNEEELLSSLKETGRILDQKRLVRAKEIEEQIRVHLEDLRMK
                     GAELRFHLKPELMPRSYGTSKVTMVVKTNPGMDLMEIGSVASGGELSRFLLALESALK
                     NQLDLKTIVFDEVDSGVGQRLGTVVSGKLEEISREIQTIVITHLPQIAMSSDRHFVVR
                     KQHVHNETISIIEELHGSSKEREIEEMSGQIPD"
     gene            168279..168896
                     /locus_tag="PKL93_01970"
     CDS             168279..168896
                     /locus_tag="PKL93_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70064.1"
                     /translation="MEREELINRIVEEKGVEAAPVLLSLLVSEDSETAQICFDALIQM
                     GKDVVPFLIEKMRSEDIDPVSRLYLADLAGEIGDIRAVTHLYELLSKYSDERSQIVIY
                     EALARLGEGEKVVDVLALMLEENYDAELQDQLIMALSNTDSSVAVKALAGFYGNKLAD
                     KSTKAFALEGIHSILARRHELKNYLLSLHNGKEIAERLYQWQKEI"
     gene            168878..169795
                     /gene="murB"
                     /locus_tag="PKL93_01975"
     CDS             168878..169795
                     /gene="murB"
                     /locus_tag="PKL93_01975"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNQ70065.1"
                     /translation="MAERNLTDYFNSIYLAGADVRLEEPLEWHTTIRIGGPARVFVSP
                     YSVESLSEILSLLKSEKLPYKIIGGGSNVICPDKYEGVVVSTRNLNLLRSDGVKVFAQ
                     AGTSINSVIWHCLAEGLTGIEFLTGLPGSVGGAVLMNAGAFGGEIGDRVTRVSYLDGR
                     GKVIDVDSKSAGFSYRNSIFKAADGIVVGAEFLLKRGERSEIETKMKEILAKRIQKQP
                     LEHPSAGSVFVRPKPDFYVGSTIERLGLKSLRVGGAEVSEKHAGFIVNRGGACQKDIL
                     TLVEIIRKKVEGATGVILSTEIEIWKEVE"
     gene            169795..170772
                     /gene="hflK"
                     /locus_tag="PKL93_01980"
     CDS             169795..170772
                     /gene="hflK"
                     /locus_tag="PKL93_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNQ70066.1"
                     /translation="MVDVRIEEPDNNFTGNGGPKKSFFWSGLFVLLVVAAIVVVYFLS
                     GFFLVGPDQVGLIKRFGKFTKSVGPGLGYHLPFPIESVVVIDTSNLRKEEIGFRTIRT
                     GTYQTYANESLMLTGDGNIVSVELVVQYYVGDPAKLAFTIVDDGDIVRFTTESVLREE
                     VASSTIDSILTTERDTISIRTAERVQEELDRLDTGIIVKNVFLQEVAPPQQVITAFDD
                     VNSAKQDKEKLIYEAEKYTNDIIPKAEGEAAQIIKDAEGYAQERILNAEGEAERFLEI
                     LEEYEKAPDVTRTRMYLETLNKVLSEASKTVVLDQSSVLKLLDLEGSVR"
     gene            170772..171629
                     /locus_tag="PKL93_01985"
     CDS             170772..171629
                     /locus_tag="PKL93_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNQ70067.1"
                     /translation="MKYKVIISVAIIVVIAIILLPSFFFIIDETEQAVVLRFGEIQKS
                     ITEAGLYTKTPFIDNVRKFDKRIQIYDVDAERIYSKDKKTILADTFALWRIVDPRKFI
                     ETMKSELTALTRIDDVVYSHVRNTFGKLDYDEIISGKRTDVLDEITALAANDMKDFGI
                     QIISVRVKRADLPDENRNAVFERMKSERIQEASLIRAEGNREAQKLRAEADKEAQITI
                     ATAQKEADIIIGTGDARALSIYAEAFNRDPDFYEFMKRLEVYESTLEDANYILGPAMD
                     FIDKLSKGE"
     gene            171629..172258
                     /locus_tag="PKL93_01990"
     CDS             171629..172258
                     /locus_tag="PKL93_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNQ70068.1"
                     /translation="MSCKKSLLEPGHFEINVRRSRFIGDSARCECEEEAKSFIKSLSQ
                     RYASADHCCWAYRISLQGREIANYSDAGEPHGSAGKPILGAIESLELTDVVVVVTRYF
                     GGTKLGVRGLIDAYGETAAGALRAGKLALYCPGLELVVEMTYSQWNDFIRLFGEDKDF
                     SVRKVDYSERVFASLAVQEKNIERITGFLEDRRIFHKSGEELSFVSPLE"
     gene            complement(172213..173922)
                     /locus_tag="PKL93_01995"
     CDS             complement(172213..173922)
                     /locus_tag="PKL93_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ70069.1"
                     /translation="MKIKRFDLFFVLFILTVGLFIYKLYDYQIVSSDKYAAQVESISH
                     RSISLLPQRGMILDRNGIPLAWDAPYYNIEKRVVFLPDDLKRMIIDAFDDKQKAETLV
                     RNLEMFGSVETSLPAALAERLNDFPELEVSERVIRYYQNNPGVAHVVGYIKADGEPVM
                     GIEKQYNDLLRGTPGEQLIRVDALGRQISLEGERPPVGGKDLTITIDSQMSKYIYELI
                     SETGKSGAAVVMTTSGEILALVSVPGFENMSFTRGISNRDWERLNLDPSRPLVNRALT
                     PFTPGSVIKPFIAMAALAEGKDATEQIDCRGSFQYKDSQGIVQGVYRDWNLYGHGITD
                     LSKAITVSCNVYFYQLGLELGIDTISNYAKIWEVFEPTNIDLPEESTGLMPCPSWKQE
                     KLEENWFPGDTIQISIGQGYLSITPLQLAKMTGEIATKGREILPYIGAERVSGRTVIE
                     LRESDWSLLTEALRNVISKGGGAAEAGTAYSAFRNFEGTAAGKTGTAEQGGSKPTHSW
                     FTGFMPLEEPEIVVTVFVHEGGYGSGLASQISRKIMDYYLQEYINQNHSSGLTKLSSS
                     PDL"
     gene            complement(173912..174292)
                     /locus_tag="PKL93_02000"
     CDS             complement(173912..174292)
                     /locus_tag="PKL93_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70070.1"
                     /translation="MIWLLLIVPALFFDLGFGDLIPLYPIYLTLLVFLPTRNVAFALS
                     LALLLSWIVGIALSGIGMVIALAIILVISAVAFQDRYRTPSFGALVAAAIGITGVCFA
                     GTGFGVLFPFFATFLILRRLSVED"
     gene            complement(174289..174987)
                     /locus_tag="PKL93_02005"
     CDS             complement(174289..174987)
                     /locus_tag="PKL93_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70071.1"
                     /translation="MKFAEVAVVILMIFLVLSSILEVTGTNRRASEFFDGMALPLINL
                     RVDIENFFRALFQTGSIDRVIRDLNRYADSFSDKFEFPIPMRVAFFSFERNNTAVMTT
                     NRPALPGTPVASFFSSTIYGVVKNSNERYLLVAPLTSPGTRISAAVHREETVIEGVIY
                     SDAGKLYFSTFDPYYLEAGDSVRIASTVPGYGYYKAMKLDLIGEIAGRHGSDYLVKNP
                     NVFGDYFLFLELQE"
     gene            complement(175002..176021)
                     /locus_tag="PKL93_02010"
     CDS             complement(175002..176021)
                     /locus_tag="PKL93_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNQ70072.1"
                     /translation="MFLRQDMGIDLGTANTLVYVKGQGVVTNEPSVVAIDVNSGKILQ
                     VGLEAKRMVGKTPASIVATRPLKDGVIADYDIALAMLKYFMGRAIGRRTFVKPRIVIG
                     VPSGATEVERKAIVDAAIEAGAKKVFLIEEPMASSIGAGLNVEEPTGNMIVDIGGGTT
                     EIAVISLGSIVLSQSIRVGGDEMDDYIVQYIKEKYRLMIGEKTAERVKMEIGNVVETE
                     EADGSYAEIVGLDLNSGLPRKISISGSEIREAIAEPVSRIVEAVKLTVENTPPELISD
                     IVQSGIVIAGGGSLLNGMQELIQRETGIMVVIADDPLTCVARGAGQVLERVSILERIE
                     KGNLR"
     gene            176295..177170
                     /gene="speE"
                     /locus_tag="PKL93_02015"
     CDS             176295..177170
                     /gene="speE"
                     /locus_tag="PKL93_02015"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNQ70073.1"
                     /translation="MEDRELRTLNHLMYMEYYTGGNAGLFMKMKQLVYSFQSPFQRID
                     IFEHPEFGMVLVLDGITMFTEADEFMYHEMLVHVPLFTHPNPKRVLIIGGGDGGSLRE
                     VLKHPSVEEAILCEVDPYVIDAAKLHLKEMSVELDNPKASIFNENGADFVRKNKDYFD
                     VIIVDSTDPTAGEGGHLFTKEFYSNCYKALRKDGIFCAETEDPFYDKGWVAIAYNRIS
                     SVFPISKMYTGFMTTYPSGMWTYTIGSKELDPLEDYDEERVLDFGAPLRYYNTEIHRA
                     SFALPTFLKELTGQF"
     gene            177338..179149
                     /gene="lepA"
                     /locus_tag="PKL93_02020"
     CDS             177338..179149
                     /gene="lepA"
                     /locus_tag="PKL93_02020"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNQ70074.1"
                     /translation="MYKRDRIRNISIIAHIDHGKTTLVDRFLDITDTVDRRNKKDQFM
                     DSMDIERERGITIKSKPVKLNYLADDGITYEINIIDTPGHVDFNYEVSRSLAACEGAI
                     LLVDASQGVEAQTVGNTYLAIENDLELIPVLNKIDIPNANIEETLAEIVDLIGYSPED
                     CLQASAKTGQGVKEILEAVVKRVSPPSGMLEAPLKALIFDAIYDKYRGVVIHVRIFEG
                     SVKAGDRIQMMASGEIFEVVEVGYFLPQMEITDSLDAGEVGYIIAVIKDVSSAKIGDT
                     VTSANNPTDTALPGYKEAKPMVYAGMFPGMPEYYDELRKALDKFKLNDASLVFEPENS
                     PALGFGFRVGFLGLLHMDVVRERLEREFEIACILTAPNVVYRVTLQNGEVAEITNPAS
                     FPEPGEFLRVEEPFVDLSIITPSEYMGNLIGFITSEKRGDFKAVENAGKNRVVLRFEA
                     PLSEIMFDFFDRMKAISRGYASMDYEILGYRESDLVKVTILVNKETVDSLSFIVHVDK
                     EYSVAKKVVDKLSELIPQHQFQIPIQAKSRGRIIARSDIKALRKDVLAKCYGGDVTRK
                     MKLLEKQKEGKKRMREIGQVTIPQNAFLAILRIGEEE"
     gene            179160..180014
                     /locus_tag="PKL93_02025"
     CDS             179160..180014
                     /locus_tag="PKL93_02025"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNQ70075.1"
                     /translation="MLIRLGAHMSISKGFDKVPGDTVGIGGNTFQIFPHSPRMWKASL
                     PKSEMSASFISEMKDKSLDPFDCMVHSGYLVNIASPNDEVWEKSVNLLSLEMKITATL
                     GLKYLNFHPGSHLGEGLSEGLERILKGVEIVLGENRDNDVMLLLENVSAKGNHIGSSF
                     EELAAIIDGSVQPERIGITYDTCHGFDSGFDIRDLEGTLKLIDKIDSTVGYEKLKMIH
                     LNDSKYPLGAGKDRHEMIGKGYIGMNGGFKEFLSNEKIQEIPWLLETPGDDADHALEI
                     KYVRELIG"
     gene            180031..181614
                     /locus_tag="PKL93_02030"
     CDS             180031..181614
                     /locus_tag="PKL93_02030"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNQ70076.1"
                     /translation="MKKYVVVTGGVISGIGKGILSASIARVMKECGVEVNTLKIDPYL
                     NLDAGTMNPNQHGEVFVTEDGYEADLDLGHYERFLGKNMRRENNMTAGQVFKSIIDKE
                     RKGDFLGATVQMVPHVTDEIKSRIRKVPGDLIMIEIGGTVGDIEGEIFLEAVRELWVE
                     EGPENFLFIHVTYVPYLRVTNEFKTKPTQQSVQLLRRIGIQPQMIVVRSELPIEENEL
                     GKIALFGGVERQMVYNLPDSENVYDVPRIVYSHGIHRKIASWLNIEIEERFEWNYPKS
                     FAPTRVAIVGKYLGTDDAYKSISESITLCGASKPEVIDSQCFEDMTDAQIKAELRKYD
                     GIIIPGGFGKRGIEGKIAVIKAAREEDIPILGICLGMQLMVIEFARNVCKLENANSTE
                     FDPSTPYPVIDLMEEQKAILKLGGTMRLGAQETPIEEGTTLYEAYNERLVYERHRHRY
                     EVNLERFPDLFRTPEETDESKLRISSRATFVEAIELPEKRFFVGIQYHPEFRSKVGRP
                     NPIFELFLTRVREKRRIQQ"
     gene            181611..182456
                     /gene="folP"
                     /locus_tag="PKL93_02035"
     CDS             181611..182456
                     /gene="folP"
                     /locus_tag="PKL93_02035"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNQ70077.1"
                     /translation="MTFENGRVLDLSEPRIMGILNITPDSFFPGSRFLDIPVALDKAL
                     EMLENGAAIIDIGGESSRPGAEKVESKEEIARVLPLIKQLRSKRPDAVISVDTYNSET
                     ASAALSEGADMINDITGLRSMKMIEAVSENHAAVVIMHMRGDPSTMSRMTDYRDVVGE
                     VSSWLLERARRAIDFGVDPDRIILDPGVGFAKSAEQSIEILKNIEKFKKMGFPVLVGH
                     SRKSFISKITGTPVEERLEETISISTYLYLKKVNIIRVHDVQEHGRIFGILKTLNPCG
                     EYWGE"
     gene            182458..183786
                     /locus_tag="PKL93_02040"
     CDS             182458..183786
                     /locus_tag="PKL93_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70078.1"
                     /translation="MPYSRRSKKYWKETWKAYLFLLPSIIILGVFVFWPIGFSLVLSF
                     FKWDYTSTTRYFIGFDNYKELFRLTYPVSLSFVNSLMNTVVYVVIVLVVVQLIYYLLG
                     LMSKYDKTDKSHSFLYVIFIALLSGYVLLRNTSIGLTTIGLFAGLGLMGFGLYVLLRR
                     KSSIQSRRMKSSQWSTLILFIVVYYVFAYQILTQSADFVQYFTLAKESSDFLKSIYNT
                     VYYVLLSVPTQIALALIIAMLLNKNIKLRSLFRTAYFIPFVTSVVAVSLVWQWMFNDQ
                     FGLLNYILSIFNLPRIAWLKDEVWTIPTIAIVSVWQHVGYTTVIFLAGLQNIDKSYYE
                     AADVDGASGWQKFKFITWPLLSGTTFFIMIITMIGSFKVFSQIFILYQGLPGPVNKSG
                     LTLVYYVFDAFYNQQRMGVASAAAYVLFMIILVLTMVQLNVGKKRVHYEG"
     gene            183796..186207
                     /locus_tag="PKL93_02045"
     CDS             183796..186207
                     /locus_tag="PKL93_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ70079.1"
                     /translation="MTTSSTKIISKILLYVVIVGGAFLMLIPFAWMVATSFKAPSEVS
                     TWPPVWTTKNAASSFTFKTQIKQKSASASVDLSALSLSEFRNFAALMEESASFDTVTV
                     NLDDDPIRRGEIEIQLLETNGSPADYSTEVDRQRFESLIERYHTLATNFPDSVSRDLK
                     DLPVDDVGRFLDSFFNVAVYGDNGFVRRVILVTSLKTEYSKAIERLAQSVETSMKELP
                     IDTDESKAIKKRIREESARLFENPVAELTRSIENLEPYRTGSTGVTDLTELEKVVEGL
                     RDSINTIRNVASEVISLSQEISAGDSRVSLSVRQLERSLEAVPSGLIQWMDLLNLYSD
                     LRIFYDESQNESLSSASIVGKIRTDSEVHSLLSDFVNRWDIDEEMRTHLLTKVNPSNV
                     RNAVAILLNYLDSNFKESLSKYFSDVQTPLEAINDAMNFLRTSLLVASDPVLDEKVNS
                     AMDEETPYSSLITLIKEVASAAGQTIGVGGVVASLDRQANISGHEPFAELIRRRWRET
                     DLIGTFSRVHSDIFSELDLSSKPDEVERVYYRGIISPSGAKSFDIKLKGIPAVWLKDD
                     VSAGKANFTLGETISNFFQNYVTAWNAAPFGRYYFNTVFVATVTTFVEIIFAAMAAFA
                     FAKMEFFGKNLLFTLFLATMMVPGEVLLVPNYITLTALGWIDSYYALIVPWVVSVFAI
                     FLLRQHFMTIPNELYDAAMIDGLSKWRFLWTILVPLSKPAVITAALLKFVGSWNSFLW
                     VLIVTKSPEIRTLPVGLQNFSSATGSDYHLLMAAATFSIIPVVILFLITQKYFIAGIA
                     RSGLK"
     gene            186243..186455
                     /locus_tag="PKL93_02050"
     CDS             186243..186455
                     /locus_tag="PKL93_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70080.1"
                     /translation="MFIFLALLFSLLLITNYNAIARYKQAQRKLENLQREYDALEKEI
                     EIKESELELLRQLAPGMPQNDTGGNR"
     gene            186452..187234
                     /locus_tag="PKL93_02055"
     CDS             186452..187234
                     /locus_tag="PKL93_02055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ70081.1"
                     /translation="MRFVVTTSHKPTREEVVNARTLAEELGVKYVSRGRLREFEKESP
                     VDFYYVFDKNGQLSIKAGESVFFFHPGMSKVRYKNIKLQNSDYLINSMELHGEEIVLD
                     TTFGLGNEALLIAHFLPRGKVVGLEASEHIFRVVSHGLRNYPYTDEWIREASTRIELH
                     NIDLREFVKSCADNSYDIVYCDPMFDRPQLSSHSINPLRPFAVYERIIRSDVDEMIRV
                     ASKRFIIKSRTRDTLFEELGLHFDRLWGSKKSGVLYGVIEKK"
     gene            187231..188151
                     /gene="miaA"
                     /locus_tag="PKL93_02060"
     CDS             187231..188151
                     /gene="miaA"
                     /locus_tag="PKL93_02060"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNQ70082.1"
                     /translation="MIPLVLGPTAVGKTSLLLEIAGRLPIEVISVDSRQIYRFMDIGT
                     AKPTRTELSLLKHWLIDIRDPDESFDVMEFRKLSLKSIAEIKSRGKIPVLAGGTGLYA
                     DALIRGLADVPARDETIREALLQIESQKKGFLRELLRKVDPKAFETIHENDLKRTVRY
                     LEVFLRTGKPLTEFHKESEMSGEFGIVILQRERDELHRRIEARVRRMIDSGLAEETER
                     LLEMGYAPELNALKTIGYAEMVQYIKCGVSLTDTSERIIVNSRRYARRQIIWFRRYRE
                     ALRIDLSTLGNEAAQVLENTILSVWGGKNG"
     gene            188144..188386
                     /gene="hfq"
                     /locus_tag="PKL93_02065"
     CDS             188144..188386
                     /gene="hfq"
                     /locus_tag="PKL93_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNQ70083.1"
                     /translation="MAEKFNLQDRFLNLLRVNRIEVKIYLEGGFQTKGMVKSFDNFTV
                     LLEDGQEQTLVYKHAIKMMVPQKYVKLTNTSGKKED"
     gene            188394..189641
                     /gene="hflX"
                     /locus_tag="PKL93_02070"
     CDS             188394..189641
                     /gene="hflX"
                     /locus_tag="PKL93_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNQ70084.1"
                     /translation="MDTLLVAVFSSEEQNMKTEILGELQELARTAGYYVTENVVQNLD
                     YPDPRHYLGKGKVDFAKRMIEAFGVSLAITRHELSPSQAMNLERLLGIPVIDRTQLIL
                     EIFAEHATTKEGKLEVELASLKYQLPRLKGFGRMLSQTGAGIGTRGPGEKKLEIDRRQ
                     AQDRISRLRKEIEELAKRREISRKKRQSSSVPLVSFVGYTNVGKSSLVSEISSEDLVI
                     EDKLFATLDTRVRKAKLPAGMQLLVSDTVGFIRELPHELMESFKSTLDEVKYSDILVV
                     VSDASDMAIKDKYSVVDRTLREIGAGEIRRIHVLNKIDLCTNERLAELEGFFPDSVMV
                     SALRSYNIEGILNEITRKLFGEKSRRTLRLTPAEFSNFMRFRNSVEVLSESFDRELIE
                     IEYLSSDEINERLISSICEEGRK"
     gene            189638..190651
                     /locus_tag="PKL93_02075"
     CDS             189638..190651
                     /locus_tag="PKL93_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70085.1"
                     /translation="MKRLLSVILVILTTGMLFSVTLLFPLKRAPEITGYFGEFRGNSK
                     NINYPEHFHMGMDYSTGSIVGLDLRSPDDSYVHQIYINHPIYGIGIALTLPEVTNILT
                     NEEGINVIFAHVNEVGDTSTLTGRKLNDLYHQLISEFGDQYVEVTFDPRELPFRKSDV
                     VAKSGNSGNVPPHLHLEVRDSTMQTIINPGFFFDTGSPTPAIEILDIRAGGKTYSFSE
                     GKPTIEMTPNTPLDLHAKVQLRHPISPKTIELYVENSLIYQIDFVSFELDEVDKVREI
                     YSSPSTESDYWFNLNSRISLSVLPINIWDDIDWTNPRDARVVVRDHWGNEASKDFRIE
                     MRR"
     gene            190657..190962
                     /locus_tag="PKL93_02080"
     CDS             190657..190962
                     /locus_tag="PKL93_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70086.1"
                     /translation="MFFKKKPRDIGRNPFYDNGNTVVVHFQCNKCGEKFRSHLRKYYD
                     VSVNYGKGKGAYRLDKEFIGSNCQNRIQITAEFNRAFKPLSFEIVGGRFISKEEYEE"
     gene            190985..192166
                     /gene="metK"
                     /locus_tag="PKL93_02085"
     CDS             190985..192166
                     /gene="metK"
                     /locus_tag="PKL93_02085"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNQ70087.1"
                     /translation="MKTWLFTSESVTEGHPDKMADQISDAILDAMLTQDENSRVAVET
                     LLATGVAVVAGEVSTKAYVDIPRVVRDTILDIGYNRAKFGFDGETCAVLTSIDEQSPD
                     IALGVDRSYEAKKNDVDRYALIGAGDQGMMFGYATNETPEMMPLPIVLAHRLARRLSQ
                     VRRDGTVQGFRPDGKTQVTVKYQDGKPVGVTAIVVSTQHDPDLTSHEIERLVVDNVVA
                     PVIEHELLLEGVEIFVNPTGRFVKGGPSADTGLTGRKIIVDTYGGWIPHGGGAFSGKD
                     PTKVDRSAHYMARYTAKNIVAAGLAERVTLQLAYAIGVAKPVSFMIDAHDTEKVDLEK
                     LRKAVLKVFDFRPAAIIDRLNLRRPIYRQTAAFGHFGRIDVDLPWEETDAVDQLKKAL
                     D"
     gene            192211..192489
                     /gene="rpsT"
                     /locus_tag="PKL93_02090"
     CDS             192211..192489
                     /gene="rpsT"
                     /locus_tag="PKL93_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNQ70088.1"
                     /translation="MPNNASAKKRVRQTAKKTMMNKAVRTKFRNASKKLYKAIEEGED
                     PKLVSSMLSEVYSTLDKAAKSGTIHKNTASRKKARLTSKVKTYVETRG"
     gene            complement(192550..193689)
                     /gene="ugpC"
                     /locus_tag="PKL93_02095"
     CDS             complement(192550..193689)
                     /gene="ugpC"
                     /locus_tag="PKL93_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNQ70089.1"
                     /translation="MAEVILEKVGKTYPNGVKAVLDANLEVKDKEFLVLLGPSGCGKT
                     TTLRMIAGLEEITEGTIKIGGKVVNDVEPKDRDIAMVFQNYALYPHMSVYENMAFGLK
                     LRKTPKPEIEERVKEAARILGIEQLLDRKPKQLSGGQRQRVAVGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRAELKRLHQNLQATIIYVTHDQVEAMTMADKIVIMKDGIIQQIGD
                     PYSVYFEPRNKFVAGFIGTPAMNFISAKLVSENGSVWVVKEDMKLLVPDDKVDKVKGL
                     VGKDITFGIRPEDIYDKMYAVAPKDEYTVKGSVDVVEPLGSETLIHANIHGDDIVAKV
                     DPKSRAAAGNKMDLVFDMSMMHLFDPETEENVLAGTHQDAAPENL"
     gene            complement(193702..193962)
                     /locus_tag="PKL93_02100"
     CDS             complement(193702..193962)
                     /locus_tag="PKL93_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNQ70090.1"
                     /translation="MSRLHELSFFSLAEAKTHFSKVVDDCEKADIIITKNGLPKAVVM
                     DYRKYVLFNRFLERVYDLYLMDAGDEAMDVEIKDLIVQVDDD"
     gene            194029..194952
                     /locus_tag="PKL93_02105"
     CDS             194029..194952
                     /locus_tag="PKL93_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNQ70091.1"
                     /translation="MAKKFFLKQPMMRKVIYALLPILIFAVGNFGWVVFSKVFLSILI
                     SVFVEWLFEKKKGKPVSEAAIVTGFLIGLILPPAVPFWIVIVASSFAIIFAKMAFGGF
                     AKNLYNPALVGRAFVYISFPAAVQQSWTPAVQSWGQGFVRWISSDIITMATPANVIRN
                     GGSVSAWRMIFGNISGSSGETAKWLIAAAAVYLIITKVASWKIIVSTLLSAFVMSGIV
                     YIFDPSKMSPFYWIIGGSILFGAVFMATDPVSAPKKEKSKWIYGAMIGSLAVIIGSFS
                     LFGAGLMFAVLIANTFASLLDYLNTPKKVRA"
     gene            194949..195569
                     /locus_tag="PKL93_02110"
     CDS             194949..195569
                     /locus_tag="PKL93_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNQ70092.1"
                     /translation="MSDRLFSVVFTFVLTTVFVLCLAGINALASGTIEKNEQLEFQRS
                     LVYVFNLAEDIDSVSDEEILRLYGNRIEEITGDQTVYIVNKDGSKELAFTVQSPGLWG
                     TITALIAFNEEGTRITGIDFVSHSETPGLGGRIDESQFKEQFRGEKISSDPSARVAVV
                     TGQVTVSKEDARVDAITGATRTSESIQILLNKAINSVLPVVREVVK"
     gene            195571..196170
                     /locus_tag="PKL93_02115"
     CDS             195571..196170
                     /locus_tag="PKL93_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNQ70093.1"
                     /translation="MADSKKTVFLANIWKNNPVIVQILGICSALAVTNNLTNTLIMGV
                     GLIFVTAFSSLTISALRKLIPRNVRMMIQVLIIATYVIILDIVLKAYLPSISKALGPY
                     VGLIITNCIIMGRAEAFAQSHGPILSFLDGVSAGIGYSLILLAVAFIRELLGFGTIFG
                     VRVMPESFTPWVIMIMAPSAFFILGAIIWIARTPIVKKK"
     gene            196183..196776
                     /locus_tag="PKL93_02120"
     CDS             196183..196776
                     /locus_tag="PKL93_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNQ70094.1"
                     /translation="MGVPLNPFVIMIAAALTSNMVLSNFLGMCSFISVSKDIKTANGL
                     GMAVTFVLTLTSVLNYIVYYYILVPLNLDYLRYIVFIVVIAAFVQFLEMIIERFSPAL
                     YINLGIFLPLITVNCAILGGVLFMIIREYNLIQSLFFGFGSGAGWWLAIVALSAMRMR
                     MRENDLPPQLVGPGITVIIIGIMALAFMGFSGMLPVQ"
     gene            196790..197890
                     /locus_tag="PKL93_02125"
     CDS             196790..197890
                     /locus_tag="PKL93_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNQ70095.1"
                     /translation="MQIVISALVIGGISALLAAILTVADALVNNYGEVSIRVNDSKDL
                     KVKGGATLLSTLASEKIFIPSACGGKGSCGLCKVRVLTDIGPILPTELPYLSEKEKKN
                     NIRLSCQIKVKSDISIEIPEELFNIREYIASVSSIKDVTHDIKEVFFDLEDEIEFKAG
                     QYVQLIVPAYGDIKGETMRAYSMSSQPSVKSGVELLIRLVPNGIVTTYVHKILKVGDR
                     IRILGPFGDFYLRDTDSDIIFIAGGSGMAPIKSIIYDMMEREVKRNAYYFFGARSKRD
                     LFYLEEMKEVERKMPNFHFIPALSDPVPEDNWEGETGLITDVVDRYLSEQGDEIDREA
                     YLCGSPGMINACIRVLIRHKVLEEKIFYDKFG"
     gene            197906..198364
                     /locus_tag="PKL93_02130"
     CDS             197906..198364
                     /locus_tag="PKL93_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70096.1"
                     /translation="MKQTPDFDRIQENMRAGNITGPGFLGDDDRNLVDIISEDQITVK
                     ELGLTNEIIADKLEMLMKEGERGFGSPVKVDDRFVVIVEESRGYIPCPFRHGHLSKKV
                     NVNVRNIALKEEIDYSPISIHLIREHGFFQGKGSPYRLDPARVAKILELV"
     gene            198371..198991
                     /locus_tag="PKL93_02135"
     CDS             198371..198991
                     /locus_tag="PKL93_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70097.1"
                     /translation="MGILNSGKNGKKYSSRNRALFTVFFVVLLIAFVSYYAFGYKMEV
                     VVPSEDVNLSDLTFNDDVFSLLGEAPFPPDQGLANGVSVDREDGESIRVFYPPEDNSV
                     VCLQFEVNSRNINVYIWKTFSVDSARSVWDTLFLVEASVLTRDLGRIKKPDYYYAKIV
                     RFGGRDQTLIWQKGKWVVLVKSPGFTLEEDEERLILTELFDSSIRS"
     gene            complement(198975..199427)
                     /locus_tag="PKL93_02140"
     CDS             complement(198975..199427)
                     /locus_tag="PKL93_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HNQ70098.1"
                     /translation="MTKILVSACLIGVNCTYRGDNNLSLKLLELVDRFVFLPVCPEQL
                     GGLPTPRPRAEVTTSSDWRAAKLVLDQFGNDVTQNYLRGAEEVLKIAKLSGVNIAILK
                     SRSPSCGCRGIYDGTFSGVMVDGMGITAELLLKNGIEVYSEDETNFLY"
     gene            complement(199424..200272)
                     /locus_tag="PKL93_02145"
     CDS             complement(199424..200272)
                     /locus_tag="PKL93_02145"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ70099.1"
                     /translation="MNRRVILLFLFMSSLLMTRSFAFFLVTYVVRAGDSIHTISRDLD
                     VSISTIIDFNNLKNPNSIKAGDTLRIPQPDGLIYEVQSGDTFDYIAKLFFSPVDELIA
                     ANNLKPDSVINPGQRIFIPMSLVNLYQYIPQSSPFRWPIYGVISSDYGWRTHPVTGQP
                     SFHSGLDIAAPEGTPIFAGSGGTVVFAGVNGGYGNMVEIQHDNGYLTRYGHMSKISVY
                     VGQRVDAGSLIGRVGSTGVSTGPHVHFEVRDPRANTMNPLSMLPTRDLMYVIRREDDG
                     TAAGGK"
     gene            complement(200269..200700)
                     /locus_tag="PKL93_02150"
     CDS             complement(200269..200700)
                     /locus_tag="PKL93_02150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNQ70100.1"
                     /translation="MRKFVRSLGNATNGIKHLLRERNFRIQLVIGAVILSLGALIQVD
                     KNDYYWLLFCTFMVLLLEGINTLIEKLADILRPYYDERVRVLKDTAASVVLLGSAVSV
                     LIGLSIILQTIFGLHFAVGLLFGIIILVVFFSLGLFGGGRE"
     gene            complement(200697..201308)
                     /locus_tag="PKL93_02155"
     CDS             complement(200697..201308)
                     /locus_tag="PKL93_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ70101.1"
                     /translation="MKQAFTQPHSVRISGIGGQGNVLMGIILAEALIIQGNWVVQTQS
                     YGAQVRGGLSYCDVLFNSEPIDYPKAHSFELIYSMHQTAVNAHVPLLHVNGVLIVDST
                     MVSSIPKEGVRMTRKIIQKPVTELTEKKFGSTLPANMVGLGLIARAGSFVTTQSLKEA
                     MTKHIKAKYVEMNAQAIDFGYSLIEKSYSIRRERIDSVGRGFE"
     gene            complement(201305..202141)
                     /locus_tag="PKL93_02160"
     CDS             complement(201305..202141)
                     /locus_tag="PKL93_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNQ70102.1"
                     /translation="MAIARLTKYLRQDRMPHVWCPGCGNGVIMKAFIDAVDKNALDPD
                     RVSVVSGIGCSSRVTGYLNFNTMHTLHGRAIAFATGVKLARPEFKVVVMGGDGDMLAI
                     GGNHFIHACRRNMDITVIIFNNNIYGMTGGQYSPTTPHEKIASTSPYGNVENSFDIVN
                     LALASGATYVARSTVFHYAQMVQFIQRALDHKGMAVVEIMTNCHTYYGRYNAMSQPED
                     MLAYFKKNTVQINKARTMSPEELADKIVAGEFVNKNADTYSDSYRKKIEELMKAERGD
                     NG"
     gene            complement(202155..202658)
                     /locus_tag="PKL93_02165"
     CDS             complement(202155..202658)
                     /locus_tag="PKL93_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNQ70103.1"
                     /translation="MSAEDLSDFLKGFSTHKPESKAESWDSYFMRLAYQVRERSSCHH
                     RKVGALIVRENRILSTGYNQPPSGFPHCDETECIRDALGIPSGQNQEICYAAHAEQNA
                     IAQAAKFGIATNGSTIYVTHRPCSICARLIINAGIKRVVFSEGYPDPLTEFMFEKTNV
                     TMTQFTK"
     gene            complement(202658..203107)
                     /locus_tag="PKL93_02170"
     CDS             complement(202658..203107)
                     /locus_tag="PKL93_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNQ70104.1"
                     /translation="MLSPHEILELAVKVEEAGVSFYRELAENVDDESKRSLFRYLSKQ
                     EDGHAETFKFLLRKFEEESLELINWDDSRAYVEDLANGSVFSRSLHDSMSNSDYQKII
                     SLAIEAEKKSIAFYESIQKNARFTTADALKRIIKEENNHIELLKGMM"
     gene            complement(203091..204230)
                     /gene="hemW"
                     /locus_tag="PKL93_02175"
     CDS             complement(203091..204230)
                     /gene="hemW"
                     /locus_tag="PKL93_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNQ70105.1"
                     /translation="MGMGLYIHIPFCARRCSYCDFCTVEYDGDLVRQYNDLLKKEVEM
                     YPASERVETLYFGGGSPSLYPVELLRDLMSFISCSFDAAPVEVTIEANPWELNIKNLK
                     QWRDIGFNRLSIGIQSSEREILERCDRPVPKNLRDRLMASRDIFENLNLDFILGLPGE
                     NESNVAGNLEMISAINPDHVSYYVFDSDHETKLMRLVHDDIIDLPRQEIVEGLHDIIL
                     DSLSKMGFSRYEISSWERGRRECLHNLKYWRNEQYMGFGVSAGSHFDRKRYVNTSDLL
                     EYRYLVNLRRRPVLELRANSVIQEVFETLFMGLRLVEGVDLSKLELPDELNNTLVSKL
                     RAHLGDYLSPDNSRIRLNDNGMDFSRSVLEKLIDIKEEMVIAFST"
     gene            complement(204230..205051)
                     /locus_tag="PKL93_02180"
     CDS             complement(204230..205051)
                     /locus_tag="PKL93_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70106.1"
                     /translation="MKRVLCLSLVLLFGILLLSETQLKLNNAGWSVSADIRAYEDIYL
                     TLALATGIPYVGVTYVDSWEEYSTGFLKLKIQDGVFRGSLLASHEGLFMGVRYVSNTN
                     QIPIFSHTALELELSTVSKMKYLVRNWVRLPLGQLNGGLSVFLHGEDSFSFNDIGLYL
                     YAKEYERSLRIRNMESNVVATVDLKTNDSLGEVGYGIGIGMNYLSFDPGLAFAGSFIV
                     PAGDLRLMLSPTVMVSMRGIDAQLLISKLGSDDSIFAGISVTNFNLNGIFMRLIF"
     gene            complement(205048..206490)
                     /gene="gatB"
                     /locus_tag="PKL93_02185"
     CDS             complement(205048..206490)
                     /gene="gatB"
                     /locus_tag="PKL93_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNQ70107.1"
                     /translation="MLRTVIGLEIHAQLKTETKAFCSCRADVFDLEPNTAICPVCTGQ
                     PGTLPVLNKKVVEFAVLAGIAMNCTINKRSVFDRKNYFYPDLPKGYQITQYFFPIAEN
                     GYLYLENGEEKRKVRIRRIHIEEDAGKMVHQGTDSITGSSGSLVDLNRCGVPLIEIVT
                     EPDLRSPSEARIFMELLRDTLRALEVCSGDMEKGALRCDANISMVDETGRSSNRVEVK
                     NINSFKFVEKALEFEQERISKALLSGEDVAKETRSWSFSSKETFSMRSKEEENDYRYF
                     PEPDLPVLVVSDEVIERIKESLPELPWQKIDRFVEQYSLPRYDASVLSSDGDIASYFE
                     EVAQVTGKPKESSNWIMGEVMRFMNDKGLTIEEVKVSPEHFKELFDLIDEGKISNKIA
                     KDIFPTVVEEKKSPREIVREKGLEQIDDDKVIEDALQKAMVNNPVAVQQFRDGKEGVL
                     GYFVGAVMKATRGKANPSKVNEIARRMLKG"
     gene            complement(206490..207890)
                     /gene="gatA"
                     /locus_tag="PKL93_02190"
     CDS             complement(206490..207890)
                     /gene="gatA"
                     /locus_tag="PKL93_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNQ70108.1"
                     /translation="MTKNPLNLRLSEIISLGDTYKFYKERIDKYNNELNAFLQVRTLS
                     EERQGFPYAIKDNILSVGTRTTCASRILENYDSPYDATVTQKLNSKGGQLLGKTNMDE
                     FAMGSSTENSAFGPSRNPWDLNRIPGGSSGGSAAAVAAGLTPFALGSDTGGSVRQPAS
                     MCGIVGFKPTYGLVSRYGLVAFASSLDQIGPMTRCSQDAADVMSMISGHDSCDSNTIR
                     HSLSFDVSLRGYLKGLKFAIPSEMLSYPGLDEEVKERFLSITRDIERSGADVEVISIP
                     SVEYAVATYYLIAPAEASSNLSRYDGIRFGPRQQSEDYEGLTSKSRDRGFGKEVKRRI
                     LLGTFTLSATYYDAYYRKALKTRRVMAGELSKVLDEYDFILNPSSPLVAPRIGEITDP
                     LAYYLMDIYTIPANVAGLPSISVPAGFVNGMPVGVLMTGKRFSDVSILDAAKGIEDLS
                     PSFSNGLAIIPERWRE"
     gene            complement(208705..209088)
                     /locus_tag="PKL93_02195"
     CDS             complement(208705..209088)
                     /locus_tag="PKL93_02195"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNQ70109.1"
                     /translation="MNFEESTVAYVRYIGPCAGDEKLFETLFNKLCSWAGPRGFLEKK
                     SASSLIIYHDNPEVTEEEKLRLSMCITVPQDTRVDGDIGKMIVPAGGYAVAHFEMDST
                     ECAQSWNWLYDVCLPESGYIANDLH"
BASE COUNT        60308 a        42577 c        50303 g        56551 t
ORIGIN      
        1 tttgcttctc cttttagcat ggaagatatg gatctgatta acctgatagt tgtcgatata
       61 acggaagccc ttgccgcgaa aaataaactt tcggaaactt tggggagatt gcacactgct
      121 cttgaaggag ctgtagagtt tgttagcaag gtagtaaagg cgagagatcc atgcacggca
      181 ggccatcaga agaacgtgag tcgtttagct actgccattg cagagcggct cggtctggat
      241 gataatagga gtagaaccgt cagaattgcc ggcttgctgc acgatgtcga caaggtttcg
      301 attccggttg agatactttc aaagccggtt cgacttactg atcttgagtg gtctttgatc
      361 aagcgccatc caatagtcgg ctacgaaatc ctttacgatg taaaacttgg tgggccaata
      421 gcggagattg tcaagaacca tcacgatagg ataaacggat caggttatcc agaaggtctt
      481 attggatcag aactatcttt ggaatcaaga atcgtggcag tggccgatgt ggtggaggca
      541 atggtttctc acagaccgta tagagcttct aggggacttg gtgaagctgt gcaagagatc
      601 aagggcaact ctggtgttct ttacgatgaa gaagtagttg acgcttgtct agctgtaatc
      661 gattcgggtt tcaagattga taggtctgat agcagattat agattgttgg aggtagagaa
      721 tgaacgtatt tattgttatt ctggcaattt tcggagggct ggtttttttg ggtcttgcgg
      781 tgaagttcgc ttttttcgga ctccgggtaa ttttcggtct tgcctttgtt gggctgattg
      841 ttttcgcgat tatttggctt ctttcgaagg tccttttact gttttagttt tttgattgtc
      901 gggaatactg aggactttgc ccttgatacc cgattcgttt caattccgaa tgggtggatg
      961 tttcaagttg aatcttcact ggcggttgaa tgctgtagta gatgggatca tttttctgag
     1021 gatagactga gaaaagtcgt accatactgc ctgtgctcag aatttctggg tagagtctaa
     1081 attttaactg ggaagtgaaa atatgaaaag gggtaatact tacatcttgt tgattgcaac
     1141 aatatcactg actgtatttc ttaccctgag cgtcctcaat ttcctcagac cttatcaacc
     1201 tagtaaagat gcctattact ctctttcgag ttcaaatgaa gttatttact gtttcaaaga
     1261 tggaactcac atcttcactc ctgcgaaagg aagtgagatt ggaatagtgt tttatcctgg
     1321 tggcctcgtg gatccagtat catatgctcc acttcttaag agaatagcca caagcggtgt
     1381 ttcatgtttc cttgtagaaa tgccccttaa tcttgccgta ctttctccaa acagagcaaa
     1441 aaatatcttg aaagacaatc cagagattag gagatggatt ttggccgggc actctcttgg
     1501 gggttcaatg gcagcgaaat ttgcttacta taatcagggc agagtcgacg gtctcatttt
     1561 gcttgcctct tatcccggta aagacacttc tctatctgaa tcaggcattc atgtactgtc
     1621 gatttatggt agcaacgata aagtacttga cattgaagct ctggagagcg gaagagctct
     1681 actccctgaa gaaacaactt acttgaagct gcgtgggggg aatcatgcgc aattcggata
     1741 ctatggacca caaagagggg acggagatgc attaataacc cttctcgagc aacaagaaat
     1801 gaccgtcagc gcaattctat cattcattga caatgcgatg taatcctcat tttgagatta
     1861 ttttgccagc tgatatacca gaacgcccga gagcattaag agcaatccaa tgactttgga
     1921 gggagtcaga tcgataggca gctcgccaaa cagtcctgtg acgtttatga taagtgcaaa
     1981 gactatctgg gttactacaa ttattacgat gcctctgctc gcacctagag aagatacacc
     2041 ttgagaaata cttagaatta tcaagacacc tatgaatcct gcggtgtagt agagtggatt
     2101 gagtcctttt agtgactcca ggctcatttc acccttaccg aagaaaaaga gaagtaccga
     2161 ggcgagaaga aatcccgtac cgtgtatgaa gaagtttgaa ccgaagaagc caattttctc
     2221 tcccagtctt gcactaaaaa cactttgcat agcgattact atcccagctg ctattgcgaa
     2281 aaatacacct ctaatcatcc catgtctcac ccatctgtct ttgaagttgg aattttcact
     2341 tccagaatca aactttcatc ttttgatgcg tatagaagca tcatagccga gattcagcag
     2401 aactctgtta agaaagcctt taatgaaagc tccgccgaat atctgtagtc tttgggcaat
     2461 tacaatatct taaggaaagc gatctacgtg tgttttgagc tgtcttaatg aagctgaaat
     2521 aactgatttg atctcttcaa acaagaaaaa cgcattaaca gaacttcttg gttttggtta
     2581 gtttctgggc atagaaaacg cattttgcta gatttgcttg agatcagccg gattaagact
     2641 tgtggaacta ctaaatgaaa ctctttccta aaaatcggcc gatatccagt acgcctgcct
     2701 tccgttctcg tcagtcgatt ttgttccaac aacgacgaat ttcccagcgc ctattctggc
     2761 aaatgcgtgt ggttcaaatg aatccacatt aaaagcctca tgagttaaga cttctccttc
     2821 tttggtgatc tctatcaatc cgaagccctc atcttcaaca gttacaaatg atgccaaccc
     2881 cacgtagttg tcattgtctt tcagaattga gagtagtgca gggtatttat cgtcaagaag
     2941 tgtttcttca acaatctcgc cgaatgtgtc aatcttcgct aaataggcct ttcctgagaa
     3001 actactggag attccgccga caaggaatcc gttttcggcc tcgattgcag ccctcccttc
     3061 gagaaaggga ccttccgaat tgccgatgag cttctgccag cgtgtctttc catacgaatc
     3121 tagcaaagcc agccataggt actgacgttc ctcgatccag ctatagccag tggccagaat
     3181 attgccatcg tcaagcttcg ttactgcctg agctccatca atgccatctt ttccgaattc
     3241 tctctcccat actgttcttc ctgaaagatc gattttgatc agactggcct gaaggtaatt
     3301 gttgtcagtc atacactgac cggcaataac aaatccatca tcagtgacga ccacactcca
     3361 gggagaatag tctctgtatt ttccgtagaa gttctgccaa acctgactgc cattcccgtc
     3421 aatcaatgtg gccattagct ttgcgccgct tgtcatctct acacgagata cgagaagggc
     3481 cataccttca tcagtcaaga ctatgcatct accgatatca tctaccggtg ttccgtactc
     3541 ctttgtccac gatatattgc cgtctgaatc agttttaata acaattgtct gcatataggg
     3601 ttcatcatag tactcggaaa agccaactgc caaaatacca tctgatcctc cgacaactga
     3661 gctcaactct tctgttcttg agcctccgaa gctacctgtg aatgcaaatg caagttgtac
     3721 tataagaacc aagatagaaa gcaaggccat cctcttcata ttcaacctcc ctcaaaatag
     3781 agatgtcagt ctgcattgcc cggagatggg acaagttctg agtttctcct cttgcaaagt
     3841 gaatggttca attctaaact agttcttgcc tgaacgatcc gaaatatttg ttgtccaatc
     3901 agtttgacag gtttttctct tctgaaaagg atttactctc cgaatttatg gttcctattt
     3961 tgcttttctc aaaacactcc agaaaaaagc ctgcgatttc ttgaactttc gcctttcgaa
     4021 cttgtaaact atggggacag tgatccctaa agggggagat tgcaagaacg atcttctcgg
     4081 gaatttcatg attcagctat tagagtaata taatcagtct atggcctgct aatacttttt
     4141 cagagggtga tgcacaaatg cttcagctga aggaagcatc gaaaatctat agaacgggag
     4201 aactagaaca aacagccctg cataaggttt ccatttgctt ttcaaacgac gaattcgttg
     4261 caatccttgg gccaagcggt tcaggcaaaa caacgttttt gaaccttatt ggtgggctcg
     4321 atagatgcga catgggtgat ctagttataa atggcagatc cacaagggat ttcaaagact
     4381 gggagctaga tgcttacaga aacaagagca tcggattcat ttttcagagc ttcaatttga
     4441 tcccacatct tagtcttctc gataacgttg aaataggtat gacacttggc ggtgtgcctt
     4501 cttcagaaaa agaaaagaga gccatagagc tgctagaaaa agtgggctta gcagatcaag
     4561 ctcataagaa gccctgtcag ctatcgaatg gtcagatgca gagagttgct attgcaagag
     4621 cccttgccaa tgaccccgat atcatcttgg cagatgaacc tactggttct ttagatactc
     4681 agacaggtga agaaactgtg aagttgataa aggaggtagc gaggggaaaa ctcataataa
     4741 tggtaactca caatgaagaa atcgctaatc gctacgcaga taggataatc aaacttaagg
     4801 acggcaaaat cgtaagcgac tcccgtcctt cagatgatga cgatcctcaa ccagaactta
     4861 ttataaaaaa aacaagtatg agttttccag ctgccctgag actttctgca accaatatcc
     4921 taacaaagaa atggcgtaca gcacttacga tactggcttc tagcattggc atagtcggaa
     4981 tctttcttgt cttggcactt tctagcggtt ttggaaggca gctatcagaa ttcgagtcgc
     5041 agactctctc tacgtttccg ataatggtga gtcagacaac agttgatctg gagtcgcagc
     5101 agtatcgcca agctgagtct gaaaagcggt ttgaagaaga tgatagtgat gtcttggggc
     5161 gatttgtcta tccatacgat ccccgtgatg agattctaat tcacaacaac gatctatctg
     5221 atgaatactt ggcttatgtt gagagcatag atcccaaact tctttcgggc ttcacataca
     5281 cgcgacatat aaacatgaat ttggttgcaa agtacggaga aatggcgaaa actgttgatc
     5341 agacttcaat caaattcacg gcgtatccgg aagatctcga cccggagtct ccgggttatc
     5401 tcgagaacaa tttcgactta gttgcgggat cctttcctaa ggaattcacc gatcttgtaa
     5461 tgatcgttgg aaaaggaaac agaatatcaa atgtgattct tcaagcgttg ggtcttgatc
     5521 atgataaaga gaggattgga tttgaagaaa tcattggatt agaaatcaag gcaatttaca
     5581 atgacgattt ctacttgaga aaagggatga gttttacgcc gaagacttct ctaagagacc
     5641 tcatggatct ttactatggt gatctcggga taatgctgaa aatagtcggc attttaagac
     5701 ccaaagcaca gattgagttc tcagttcttg acgaaggaat agcatactcc gatcgtctcg
     5761 cccagaaatt cattgagaat gccgtagatt cggagattgt cctcgcccag aaggatcttt
     5821 atgtaaatgt tctgtccgga gaacagtttg cttcggattt gttcaatgtc ctctcagtga
     5881 taccacccga tattactgca agattggtgg gcggcgtaaa tctacccata acgaaaagaa
     5941 atattcttca gaagctcgga gcatacaaaa ctcctgtttc cgtcgtgctt tatcccaaag
     6001 atttcaaatc caaagaagag gtcttgaaat acctggactc ctggaacgaa ggcaaagccg
     6061 ctgagaatag gattctttac attgatctcg cgtcaacgat aacgggatta ctcgcgggga
     6121 tactaaatgg ctcaacattc gtgttgttgt ctttcgctgc tatttcgctt gtcgtttcgc
     6181 taataatgat tggaattatt acatttatat cggtgactga gagaacaaaa gaaataggga
     6241 tactgagggc tcttggagca agaaagaagg acatcagtac cgtgttcaac gcggagaatt
     6301 tcatcatagg agcgttttcc ggggcccttg gaatattgtt tgcttcgata ttgatcgtgc
     6361 cacttaactc aattatcgaa agacttaccg gtctatctaa cgtggcctat ataaatcctt
     6421 tttacgtgat ctctttaatg gtggccagta ttctattaac agttttgggg ggcctgatac
     6481 cttcaagaat ggcatccaga aagaacccag tagatgctct gagaattgaa tagagagagc
     6541 agctccttgt tttttgtggg gttttcgctc tgttgtctct gaagtctctt ctctggtaaa
     6601 gacaacccat ccgaccaaag aaactaagtt ctcagttatt ctttcctcag tcaaagatca
     6661 ggatcccggg atatagagat ccagagcttc agagatattt gtgatttcta ccgaaggcat
     6721 cttaactaac ttgccggaac caaatccaaa aaaaagaagg tatatgtttt ctggtgctcc
     6781 agatctaaca tatgcatctc ttccaaccat tacagtgtat tttgtttcaa tttcgggatt
     6841 cttgaaatag aagaactttt cttgagaaag tccgggtttg agatttttct gaaagaagta
     6901 agctgtatct gcagagtttt tcttcacccc gacccagact tcttttgttc cagtcacgaa
     6961 tattctgaag tacggtctgt cgcttgaact ttgtgcttct gtaattattg tttccaagtc
     7021 aatagcatag ttagacagaa agttcttcgc aacgatatac tgctcgagcg ttccgcccga
     7081 tccgagaagt ctttcgacac ttgctagttt agcttcaaaa tcatcctcga ttgctttaag
     7141 atcttcatcg tactgcctgc gcatctgtga aagctccgcc ttcatctgtc caactctgtt
     7201 aatggagatg agcgaaaata cgaaaccgat tatggcggcc attatcaaaa ataatccaat
     7261 tgaaagtgca ggagatgttc ttcttctctt tgtcattctt cacctctttt taccgcataa
     7321 gaagagatgc tctgaacgag ccaaggtttc gcggttcctg cagtatttga gttcccaatc
     7381 cactgcgctg tcgatttttg ctgccagcgc ttgttcgtca tatggataga aggcaaccac
     7441 cgatgaaata tctatgaggt caaagccatt catgttaagc aaatgcccaa tttcctccgg
     7501 aaaaaagggc tttgtggcaa agactatgct actaactgag ctgtctccta ttggaatttt
     7561 gtccttttca accaatctct ttgccataga gtactctccc cgcgaaagga aatcatgcaa
     7621 gaaggcccac gcattatcaa ccgtggcaag aagtagccca ccccttttga gaaccctttg
     7681 gcactcagcc aagacttccg atgtgttctt tgcgtatgaa aggaggtccc ccattgccag
     7741 cacataatcg aagcattcct tttcaaaagg aagcctttcg gcatttgaat gaacgaaatc
     7801 aatcttaaga tcggccaggc cagctttcat ggaggcgaca ctaagcattt cctgcgaaag
     7861 gtcgactccc acaacctcgt gagctttctc tgctagatag agggcccatc ttcccgtacc
     7921 agtaccaatg tcaagagttc ttccgattgt cttcacatgg tcttccagca gtctttcgac
     7981 aatacgatga tataagttcc agtaaggttc ctcatacatg tagtcatatc tgctagcaat
     8041 ttcactatag tattcccagg attccctcaa accggcgcac ctcacaaatc agcgaacata
     8101 tcaggacagt ttactatagt ataatacata ctcgtccacg attcaggagt ttgggcacga
     8161 tggcaagaat ctgtgaagac cttaatgagg ttgcttaaag agcctttcta gctcgtgaga
     8221 agtgttacca agagaattga actgacacta aacgattata agagaaagag tgtctcttaa
     8281 gtagattgtc tgttcccgca atcttggcgg ctagactcaa gttctgactt cacgattaga
     8341 tcttcttctt ttgacgaagc gattgattcg ttaatcaggg aggaatttgt gagagtattg
     8401 ataaattgtt gaacagaatc gaattgatct gttctcagca cagcaaacat gaattcgacg
     8461 ttcttgctat cgaaaattgg gctcattcta gcggcgtaga agttctttct ctttcacgaa
     8521 gaactcaccc agcttgttac ccatatcctt cacaaatgtg tttatgtctt tagacctttt
     8581 caggtacctc caaaatagca atattccgat cgttgcacca aaattgttga atatgagatc
     8641 gagcattgtg tcgttaagac tttcttgagc catgcggtag ccgggatatc tgccaaagat
     8701 tttgtcggaa atgaactctc cgatttccca aaagacgcct atcatgttta caattgatat
     8761 tcctgaaagc cagaccttcc actcaacagc ttttggagga aggttcgacc aagcgattgc
     8821 cgaaccgaaa atgaacggaa agagtgaaaa acctccccag aatccaccaa aacaatgaag
     8881 gaaatcatcg aaaaggggaa agttgttgta gaattcaagc catattccaa agaaactgtg
     8941 gagagttaag tgcaacagtg taagaaaccg tgtctcctcg tagatgggaa ctccggtaat
     9001 accttggaat atccaggttg aataaccaaa agccgccata atgaggtaac caaccatgtg
     9061 aggtaggtta aagtcacgaa tagatccaaa aactggcaca aacaggagaa acgaggcaag
     9121 tatattgatc catttaagcg ttttgtagaa cttgatattg cttgaaagaa tggatttgtt
     9181 tatggtaggt ttatctacta aactctcacc aaagcttcgt gcatatgcgc caaggattgt
     9241 aagtacgtcc cttgcagtca tttaaaccct tcaacctcca tcgaacactt tttggtttaa
     9301 ttatacacga gcttgttggt tcagcatcac aaatgcaaca taacagctcg gaagtgaaaa
     9361 acaccgcaaa tatttacgcg tgtttgtcga aatcatttgg tacactattc aagaggagaa
     9421 gttatatgaa acccaaaaag agctttgact ttgatgagtt cgccttggta tattccaaat
     9481 atagatatcc caacgagaag ctggtggagc acattactaa tagaattaga tatgccgaca
     9541 gaattctgga agtaggttgt ggcacagctg actatttgag tattctctca gagcttctct
     9601 atgctcatgg tttcggattt gacaccagcc cttcaatgat ttcagagggt tcaaagaaga
     9661 atcagagaat tgaactcgct attgcagatg caaacggtcc ttttccatat ggaaattctg
     9721 aatttgactt cgtcttcaat attaacctcg tacattatat ttctgatctt gaaaatctgt
     9781 tcaaggagag ttttcgagtc ctgcaaaatg atgggagcgt attgacagtg acggactcct
     9841 gtcaagacat ttctgagaga accttaacca gttatttccc tgagactctt gatatagata
     9901 gaggtagata tcctggaaat tcttcgatta tttctgcgat gaagaaagca ggcttcgagg
     9961 gaatatatac gacgaaggtg aagacctctt ttgaattcaa gaaacagcac tttgagcagt
    10021 acagaaacaa ggcctactcg tccctgagat tgatacccga agagagtttt gaagagggaa
    10081 ttaaaaggct agggtccgat atggaacggg gtcttgtcct cggaaagaag gcgtatactc
    10141 agatatgggg cattaaaata gcgaagaggg attttgatct cccaatgatc tacactacaa
    10201 gttttcactg aaaagtaaaa ctggttcctt taggaggtat cgtagtgaga aaattggtag
    10261 tggtctctat aatcaccatt ctttcagtgt atggcttttc tatggcagtc agtgtcggaa
    10321 tagggttcaa tttggacatg agttcgggtg ccttggttac accggtattc attcagtttt
    10381 cggcaggtat cccttatctt caggggacga tccatgcgta cgttttttct ttcgactggt
    10441 ccagcctcgt tgtcaaaggc ttcagtttgc ctgaccacac atatcttgga atccagacca
    10501 aaatcacagt ccacaagagc atttatgtga agggacagat cttctggtct ttaaaacaca
    10561 tcacttctct cattggagga gagagaacaa tggccggcac acctctttac acaagaattg
    10621 gtctcggaag caatgtttca agcataggat tggatgcggg gtttgatggg tactggcaac
    10681 tagagccgtc tagtacggtt ccatttgcgc gaccttatct tctcgttaac ttcactttct
    10741 aaaagtgact tccttcatct gttctaaatg cgggcggaaa tttcctttag gatcaaaagc
    10801 cttcaaatgc tcgtgcggag ggctttttga cctggaattc gagtctcatt ttccagttgc
    10861 cgatattgaa cagcgaccgc acaatatgtg gcgttatcgc gaagcactgc cgatcgaaag
    10921 tgatcggtcc atcgtaacta tgtctgaagg ttatactcca ttgattgaag agaaaatatg
    10981 tgggaagaat gtgtttatta aaatggatca ccttcttccg actggttctt acaaagacag
    11041 gggcgctagt ctcctagtaa gttatgcaag agaacaccaa gtttcatctt tagtcgagga
    11101 ttcttcggga aatgcaggat gctctattgc agcatattct gcacgagcag gtatctcctg
    11161 tgaaatattc gttcccgacc atacttcggt tgggaagctc agacagatag aggcttatgg
    11221 ttcgaaagtc cgattggttg aaggctcgag agaagatgta tcaagaaaga ttctcgagca
    11281 aactaaagaa agcttttatg caagtcatgt ctataatcca gtctttttgc atggaacgaa
    11341 gactcttgcg ttcgagatct gtgaacagct tggctggaat agtcctggag ctattgttgt
    11401 tccagtagga aatgggactc ttttgttggg agcatctatt ggatttgggg aattgtattc
    11461 agccggaatt gttgataatc ttccaaagat tattgccgtg caagcagaga actgtgcccc
    11521 gcttcttcgt gctttcgaaa tgaatcaaaa agattctgct gatgtaaacg cggaagcgac
    11581 tgtttcggaa ggaattgcga ttgaaaagcc cgtaagaggt gctcaaatat tgaaagcggt
    11641 cagagacagt agaggcactt ttgtgggggt ttctgagcag gagataattg aaacctggaa
    11701 ggaaatgtca aagagaggtc acttcgttga accaacttcc gcggcaacaa tagcgggttt
    11761 aaagaagtac atttcgaaaa ccgatcctaa cgaaacgatt gcatccgtat tcacgggtca
    11821 cggccttaaa acagcctgcg aattattctc ctggaataag caatagggtt tgaactgagg
    11881 tagttaaagc aagaattcgt ggatcaattt ttgatccagt ttctttaaat gagcccggtg
    11941 ggtgtggaac tgaggaaatc acttcgagat tctcctggtc gaaatgttta taagttttga
    12001 gaagttgccg cattgattcg gacctattga aggcgggagg acaccccgtc ttagtgttgc
    12061 gttgactgtt gaacacaacg gttatataat taaaaatagg aggtggttaa gttgttttgg
    12121 gatccaactt ttattattct cattcctgct ataatcctgg cagcttacgc ccagtttctt
    12181 gttagttcga gatttgcgca ttattccaaa gtgaaatcta cctttggact taatggaact
    12241 cagcttgcga ggcagcttct tgacaatgca ggactatatg atattaaaat tgaacgaata
    12301 cggggaaatc taacagatca ttacgatcca aagaatagaa tcgttcggct ttcagataca
    12361 acgtacaaca gctcctctat agcagctcta ggtgtagtgg ctcacgaaat cggtcatgct
    12421 attcaagata aggagaaata tgtacctttg attgttcgca acgccgtagt ccctgttgcg
    12481 cagatcggtt cctctctttc gtggataatc tttattattg gacttcttat ggtttcgcca
    12541 gtccttatta gaatagggat tctggtcttt tctgcctttg tcttcttcac gctagtgacg
    12601 ctgccggtcg agttcaacgc aagttcgaga gcgaaaaagc ttctctcgtc aatgggcatg
    12661 ccttctaaag agcttgaggg cgtaagttca gttcttggag ctgcagcaat gacttacgtt
    12721 gcatctgcgg ctaccgctat tcttcagtta ttcaggatgc ttttcttgtc gggtgctggc
    12781 agagactgat gcagggagct cgttccattg ctttacagac cctatctttt ttcgatgcaa
    12841 atggttatat ctcctttaga aaattggata tagcgctgtc cacgctttct tcccaagatc
    12901 gctcgttttg catgaatatc atctacgggt gtctgagaaa gagagttagc atcgacttcg
    12961 aactgtctcg ttttctcaca aaaccaagca aattgcccca tgctgtgctg aatgcgttac
    13021 gaatcggcgc atttcagatt ctttatatga aaagcatccc ggaatacgca gcactgaagt
    13081 cttcggtaga tatgattgtg gtgaaagagt ttaagggtct tgtgaatgcg gtactcagga
    13141 agctaataaa tggaggccct gcgaaaagaa aacctcttaa catcctctat tcccatccgg
    13201 aatggcttgt aaattactgg agggaatttg cctggataga cgactttgaa gagtttctgg
    13261 agcacaatca gactccccct gttcaaacag ttctttcctt gggaagagag aatgagttga
    13321 tcaaaaacgg ctttatattc gacaagagtg agtattcgga tctatcctgt gtttttcaaa
    13381 aaggtagtag tatagagaat cttcagatca tcgacgaaat tgaatacttg ctttcaaaaa
    13441 cagctatccc ggttttgact cataagggaa gtcttacagg aaaaatcaac tcaatgccat
    13501 ggcttctcca cacgttgact ccggagaaaa ttgacggcta ctcaaaagtg gctgtggaat
    13561 cgctcggcaa cttctcaaga gagcataatg agttcatata ctatagtcag gcttttactg
    13621 tagaggaaaa caagcatgcc ctcgacgttc tcgaaggttt tgaacctgta atgatggagg
    13681 acttcttcgc tgagcataag atctctgcga gatttgatgg caaaggatac tggctacaac
    13741 catggaaggc accggccgca tgttatctgg caagagttag gagtgcaaat tgaaggacat
    13801 tctttctttg gatctggacg agctgagaag gggaataatc tcattcggag aaaagaacta
    13861 cagggcaaat cagattttca agtgggttta tgtgaagagg gtccttgact tttcagagat
    13921 gaccgatctt cctatgagtc ttagaaacaa gctcggttcg ctttttcgat ttaccacgat
    13981 gaaagaagtg gaaaggcaga tttcgattga cggtacagag aaattcttgc ttaagttgga
    14041 agacgataac cacattgaga cagttgtttt gaaacatccc cgccacatca ccttttgcat
    14101 ctcttctcag gttggatgcg ctctcaactg ctccttctgt gcaactggag ccagtggttt
    14161 cacaagagac ctttctgcga gtgagatagt ttctcaggta attcttatgg agaatagcat
    14221 cggcagacca gtcgataata ttgtatttat gggaatggga gaaccttttc ttaacgaggc
    14281 caacgtctac aaggcaataa agattcttca tgatcccgta ggtagaaacc tggggattcg
    14341 tcatttcact atttcaaccg ctgggattcc cgaaggcata aagagactgg ccgattcggg
    14401 aatggacgtc cgactttcag tttcacttca tagcgcaagt gaagagacac ggagttcttt
    14461 gatgccaatc aacaagaagt atccactcga ttcattgcgg aaggtgcttg attattacca
    14521 gcaaaaaacc ggtaatagaa ttacgtttga atacgctctg ataaagggag tgaatgattc
    14581 tacggaagat ttgacaaaac ttggggaatt cctgacgggg ataaaatcct tcgtgaacat
    14641 aatacccgtg aacccagtga agccggtttt cgatagacca tccgataaag aaattgctgt
    14701 cttctcagag agtctcaaaa aggccggatt cgaatgcgca gtccgacacg agaaaggcac
    14761 tgatattgac gctgcatgtg gtcagttaag gcagaaaaga agggaatagt tttggagaga
    14821 agaaaaggag tggtagtgag atttggcagc agaaatatgg aggttgttga tgctgaaaca
    14881 ggcgggcggc taatatgtac tatgccagga cgttttagat tgcaggggat cagacctatc
    14941 gttggagata gagttgaata tgctttaagc ggtgacggtc aggggcgcat agaaagcatt
    15001 cttcccagaa agacggagct tctgcgtcca cggatctcga atatcgagca aatattgctt
    15061 gtgctttcgt tgaaggaacc ggcagttcaa aatctcatta cggatcgttt ccttgttctt
    15121 gccgagtacg cgaaactgcc tgttacgata gtagtcaaca aaatcgatct aatcaataat
    15181 gaagaactag ataggttttc agatgtctat ggtgagcact acgaaatctg tccggtctct
    15241 tctaagaacg gaatcaacct cgatcgtctt cgagaagttt tgaaaggtaa gataagtgtt
    15301 atggccggca tgtcgggagt gggtaagagc agcctcctaa attctcttaa cccgggactt
    15361 aagctaaggg tttcagagat ttcaaggggt ttggagaggg gtagacatac gacctcttat
    15421 gttgaactcc tgcaattcga gtttggagga ttaatagcag acactccggg ttttgcaaat
    15481 cttgaactgc cagtgattgc tccagaaaac ctggacaagt gtttcccaga aatctcccag
    15541 gaacaaggaa tgtgcgcgtt tagtgactgc gtccatgtcg atgagcctgg atgctacgtc
    15601 aaggagcttg tggaagccgg ttctattcat agaagtcgtt acgaaagcta tttgattatg
    15661 tataatgaat tgaaggaaag agaacaggag aagggagggc gaaaatatgg ctagaatctc
    15721 tccctcgatt cttgccgcag accttactga actcgcacgt gaggtcataa gagtccgtga
    15781 cgctgattac ttgcacatag atgtgatgga tggaatgttt gttcccaata taacttttgg
    15841 cgtgccgatc atggaagcgc tgggaagact gcatcatcct ccccttgatg ttcacttgat
    15901 gatagaggat ccttccagat acgtgaagga gtatgccgcc ttaggcgcaa agaatctaca
    15961 cgtacatgtt gaaggtaatt atcatcttca cagacttctt ggacaaatta gagaaaccgg
    16021 ttcgagagca tttgtcgttc tcaatccatc tacgcccgtc tcccttctag atgaggttct
    16081 ttcatgtgcc gatggtgtac ttgttatgac cgtaaatccc gggtacaccg gccaagaatt
    16141 tatacccgaa gctgccaaaa agatcgatgt gctagatcgt attcgaactg aaagaaactt
    16201 gaagttcgaa atcgcagtgg atggaggagt gagcctcgac aacgctcagg atctcgtaaa
    16261 tagaggtgca gatattctta taatgggagc tgcagtcttc agatcggaaa atccatcagt
    16321 tgttgtaagg aagatcaagg agctcaagag gtgaagctct acttagtgac ttctaatgaa
    16381 aacaaactca aagaggtacg cctcattctt cctgaaggat ttgatgttga atccattgaa
    16441 gcaatcgctc caatgaaaga catcgttgaa gatgcaggga ccttcttcgg aaattcattg
    16501 aagaagatcg aagcttacaa ggatgttgga gtaccacttc ttgcggaaga ttcagggctt
    16561 gtaattgaat ctcttggagg atttccagga gtgaattctg cgagattcat ggagaacagt
    16621 cattactctg aaaagatgca gatcattctt gacagaatgg tgaacgaaga aaacagagca
    16681 gctcgatttg tctgtgctgc gctgttttat gatccttcga acagcgtttt ggttggagtt
    16741 gaggagcaag ttgaaggaat tattgcaagg aaacaagccg gtgaaaacgg tttcggctat
    16801 gatccaatat ttgttcccca aggttattct caaacctttg gtgttcttgg ggattcaata
    16861 aaaaaggagc tctcacatag aggtaaggca ttcaggaaac tcttttcact tgtttacctt
    16921 tataccggga aaaaataaac aaagctattc ctactatgag taagattaca gaaactgctt
    16981 gagcagttct tacttcgctt tgacctatgt aaaggctatc taacctcagg ccttcaatga
    17041 aaaacctaac aattgaatat aaaaccatgt aaagagcagt tacttcacca taattctttc
    17101 ttttccccaa ataccagtat agtacagcga aaatgactac attcgccagg ctttcgtaga
    17161 gaaaagttgg atggaagtat tcaaattcgg agtaacccgg cattcggtat cttaaaggta
    17221 cgaacatctt ccaaggaaga tcagtgggtg aaccgtatgc ctcatagttt gtgaaattcc
    17281 cccatctacc aatggcctga gctaaaggta atacagctgt gaaaatgtca gttacctgta
    17341 gaaatctcaa gtctgccttc tttctgaatg taacataaag aaatgtaaca aggagagcgg
    17401 caaaaaaagc tccatgtatt gccaggccac catcccacgt tctgaagatg ctccagaaat
    17461 ctccgcgata gatctcatag ttgaacgcaa cataatagat tctggcgccc agtacgccaa
    17521 aaacaattcc aatgaagact ccctcgatta agtaatcttc tttgatgcct tcctttagac
    17581 cctgacgccg tcccaaaaga taagcagcga tgattccggt ggcaatcata actccatacc
    17641 atctaacgga aaaggggcct agattctcaa caattacagg attcaggatc agttgaccgc
    17701 tgaagactgc tctcaaaaag aaaaatagtg caaccgcaga ggctgatgct atcacaattg
    17761 aaactactat cttcttcact caaaccccct ctttccgaca cttcaaaacc agatctatga
    17821 gtattccaga agtcagaaaa aacctcatct attccttagg aggagcttct tctacgccgg
    17881 attcacttat ctccagttgt tcatcggatg aaggctccat atcactaata gaagcttttt
    17941 cgatgacatt gtccagaacc ttgtttctga tgatagttga aacgatgtct tctttaacat
    18001 ctcgacgagt attaaccatt tcttttgctc tttcagcgga aattccccaa tatggagcca
    18061 tttcttcggc ataagcttga atctcttcct cttcagctct gaagccctct ctcgatgcta
    18121 tttcatcgat gaacctagct ttctttattt catcaaacac accagacctg agttcttcga
    18181 tgagtttctc ttcgcttcca gcctgtttaa ggtatttttc gtaacttttc tccttcttgg
    18241 aattctctat cgttctctga acaagataat ccagagtcgc atcggaaatc tcaatctcga
    18301 caagatcatt gattttgtcc atcgcttgct gccttaagaa gtctttcttc cagttcaaga
    18361 atgattccat tccctctctg cgaacaattt cctttaattc ttcaagagta tttgcttcgg
    18421 cattcacggt ttttgcaaat tcatcgtcta gatccaaagg actctttcgt agaacgtcga
    18481 ggacttttac tgaataaaaa tattcattat cggagtcttc gaatgttctt gtaaactcta
    18541 cgacgtcatc ctttgttttc cctatgatat tcttgactat tggtctatca tcttcaggaa
    18601 caacgctcag ttcctgaacc ttattctctg cgattttctt gccatcctta attatcgtgt
    18661 actcaatctt tactaggtca ccgttttcaa caggcccgtc ttttggttca actatagcgt
    18721 gttcgtttct aagttcttcc aggcggttgt tgacgtaatt cagaactact tcatcctgtt
    18781 ttggaacttt aaggtcaaga ttttcatagt ctcggatttt tacctcgggg tctctgtgaa
    18841 gcttcacctc aattacggca ccttcacctt ccatcttttg ctcagtgatt actgcgggaa
    18901 ccaaaatctt ttcgtccttc aattcttctt caattttatc ggaaagaact tcgagaacca
    18961 tttcttcaaa gctctccgcc aagaaatttc ttacaatact tagcggaacc ttgccttttc
    19021 tgaacccagg tatagtgtat tgttgattta cgtatcttgc aacatcattt tctgccttcc
    19081 gtatttcttc cgaatcaaaa gcaaagagaa atgtctcgac attctcttct tttttaacga
    19141 agcttttctc caccagaaac tacctccatt caccttgaat taatattttt gccgtctaat
    19201 tatacgtatc caaatgaatt tcacggttaa tgagatgtta taattgaaaa ttcatcatcg
    19261 gagctggatc aactcatact ccagattgtc cggatcaatt acaacaacag tcggtttacc
    19321 cgtgagatag ccgcaagact cccctggatt tatgataaca ggccttgaat tcaacctgat
    19381 atcaagatga tgcgtatgtc cgtagaaaag gaaatccgtg attaccgctc cgcttaatcc
    19441 aaaaggttcg tgcattaaga ctaccgagcg accatgaatt ttgatctctg cgggcccctt
    19501 tatcagagag ctaccaagaa tgttcttcag cccgacaatc tctccgtcgt tgtttcctaa
    19561 tgccacgtgt agttcacctt tgaatcttgt cagtgccttt gcggcaaagg gggacacaag
    19621 gtctccgcag tgaataagaa cctcgacatc tcggtcaaca gcaatctcta cggactccga
    19681 aattcttggc atattatcat gggaatcgga cataatcatc cacttcagcg ctctcacccc
    19741 ttgatggttt cactttgttg aaaagttatg gcagatcaaa tacaattata actgcacgcg
    19801 acctgaagaa caaaggtttc ctatctgagc gtgccggagg tttaattgaa taacaataaa
    19861 ctggtagttc gcttctgttg tggaggtttt ctgcggtttc tatcccacca ggaaagtgct
    19921 acagcaatag aacggacaat caaaagatcg ggattgcctg tagcgtatac ccagggattt
    19981 cacccgagaa ttaagatcag tttttcaccg gccttgccaa cgggagtgat gagtctcgct
    20041 aattacgtcc ttctcgaaac cgataaactc agtcagaacg tggtgaatct gctgaactct
    20101 gtttccaact tcactatcag agctttaaag gcctggtatt tgccccgtga ctatgcgaag
    20161 attgatgatt tattagattc ttatagaaca gcattaattc ttcctaaaag gagttattac
    20221 ccagagcgat ttgatgctag tgcaatagtc accaagaaaa caaagagcgg atataagaaa
    20281 tacaatgctg gagaagtgtt tatggatctt tcagttacct cccttagaac agtccatgtg
    20341 gtaaaatatt cccagccgat agagtcgatg gttacagcgg aggaaattct taagatatta
    20401 tctaaagaag gcaccgcttc ttacgaaggg gtaattgcat ttgtggaaga tggaatggtc
    20461 aatggtagat ctacttcaga tattctggat gaaataggag ggatatgata tgtccgggca
    20521 caacaaatgg gcgaatatca agcacaggaa gggcgcgcaa gacgcaaaga gatcaaaggt
    20581 cttcacgaaa attatcagag aacttatggt ctcggcaaga gagggtgggt cagatcctaa
    20641 tacaaacact tctcttcgaa gtgccataga aaaagccagg tctgcaaaca tgccaaaaga
    20701 caccatggaa aaggcaatca agaaaggcgc gggtgaactc gaaggtcaat cgttctccga
    20761 ggctctttat gaagggtacg ctccgggagg agtagcgctt cttattagat gtcttaccga
    20821 caataagaac agaacggccc aagagataag acacaccctt tccaagtatg gtggatcaat
    20881 ggcggaaagc ggagcagtca gctggatgtt tgaaagaaag ggagttataa ccgtagcaaa
    20941 ggagcagatt tctgatctcg aggagtttca acttcttgcc atcgaggcag gtgccgaaga
    21001 cattaaggat gaagaagatc ctattataat agtaacttct ccagagtctg tatctgatgt
    21061 gaagactgca ctcgaagaaa atggttacac tctgtcttat gagatgagct acattcccag
    21121 taattctttg aaggttgaag gttcagatgc ggcaaagcta cttaagctcc ttgatattct
    21181 tgaagataac gacgatgttc aggaagttta cgacaactcg gatatagatg aaacagaaat
    21241 ggaagcccta gccgaacaga tgggttgatc ggccgcttgc tgttcttgcg tcacgtggag
    21301 tgataataat tgctgtctct tttggggatt acgttggctt taataatgga tcaagtgagt
    21361 aagtacttcg tggaaaacgg aatgacttac tttcaacgga tagatctgat tggggagctt
    21421 tttggcctca ggtacgttag aaataccggt gttgcatttg ggcttttcaa gaatcaggag
    21481 ccatggttgc ttgcatttgt tgccatcggt attgcggcta tgatagtaat tgtttctaat
    21541 gctcactctt ctaaactatc cggatgggaa tcctttctca ttggtttgat tgttggcggg
    21601 gctcttggaa acaatttgat agatagactg aggcttggac atgtggttga tttctttgaa
    21661 ctaaagggct ttcctgcgat cttcaatttt gcggatttgt gcattgtttt tggcgcagcc
    21721 ttgttaacac tctctgtata tcgaagggag aagcgtgctt caagagataa tagtctccag
    21781 ccgtgaagat ggctggagac ttgataaggt agtcgttgag aaagctccgc tttgggtttc
    21841 ccgcactttt gtgcaacggc aaataaaaaa ttccaaggta tttgttaaca gtagacccag
    21901 aaaaccatcc tataaggtta agatcggaga gtcaatcact tttgaccttc ccgaaaaacc
    21961 aaaagtcgcg tctgtagaac ctgaggccat cccgctaaag attgtctttg aagaccgcga
    22021 tatcatcgta gttaacaagg atcctggtat cattgttcat ccacttccaa gaaagcaaac
    22081 aggtacgctt gtaaatgctc ttctctatca ctgtaaagat cttcaaggaa taggtggtgt
    22141 aactagaccg ggcattgttc acaggctcga taaggatacg agcgggataa tcattgtcgc
    22201 caagaatgac cttgcgcatg tttcactttc aagtcagttc aagagcagat tgactactaa
    22261 agagtatatt gcgctcgtca gaggaaagac accaccgttc ggaaagattg attactcgat
    22321 tgctcggcac ccagtcaaca gacttaagat gtcggtaaat gagaatggca aggaatccct
    22381 cacatatttt caaactctct cgaatttttc aagtattgct tcattgataa ttgtaaggcc
    22441 taggacaggt agaacacatc aaataagggt gcatatgaaa gagaagggct tcccgcttct
    22501 cggagatgcg gtttatggaa aggcaagaga agacgagatt tttggaatta agagacagat
    22561 gcttcatgcc atgaaactaa cggtttctca ccctcggtca ggaataagaa tgaccttcat
    22621 agccaggctc ccagaggaca tgaaagaggc tatagttaat ctttcagaac ttgaggcgaa
    22681 aagatgaaga ctgcctttgt tattaccaag tacgaacttc atgcttctgt ccttgaagag
    22741 atatcgacta cagctgaact gaaaaaaagg ggttacgaaa gatgcgtgat tcttgattca
    22801 tcactttctt cctacgcgaa gtggcatctt tctctttcca gatctggaat agtagcagta
    22861 ccagggatta gaactgggaa cgattattgg attgcgagaa atgaaagggg ttttgaagaa
    22921 cttctaagaa aagaaacgaa tgattcagaa aacttgattc atatctccgg aaaaccccga
    22981 aatctagaca gttcttcagg aaatcccatt tgggaatgtc gttaccttga tcatgaaagc
    23041 gagaaattcc gggtatatgc gtcgttacag cctgaaattg aggtagctga ctattcactg
    23101 ccaaacgaca agaattatcg cgagcttgag cacgatctac ttcacttact ctcagaactt
    23161 ccccgcaatt tctctttgaa gaggattgag catgtttttc cggtgagagt gtcggcagaa
    23221 tctttctctt ccttattgtg taaagctctt gaggagaaaa aaatgggcga cgaatacttg
    23281 tcgaggctta aaagggagct ctcagtaatt cttgcaaaga atattcagga ttatttcatg
    23341 accgtttcca agatcgtcga catcgcgaag agcattggtt gctggatagg cccgggtaga
    23401 ggttcggctg ttggttcact tgtaagttat ctgcttgaaa ttacccgaat cgatcctgtt
    23461 gcggaagacc tgttctttga gcgatttctt agcttgaaga gagctgatcc accggatatc
    23521 gaccttgatg ttgacgacaa atcaagacca gtgctgctga agaaaatgta tgaatacttt
    23581 ggaaatgatc gtttctgtct tatcaggaca tattcaactt acggattcaa aggagcagcc
    23641 cgcgaactcg ggaaaaaatt ggggatcagc aaagataaca tctctaagct cattgaatgg
    23701 agtaaagatg ggaagcgctt tccatatgct tttgaagaag atttagacat gaagcggttg
    23761 tttgatatgt caaaactcat cgccggtctc ttttcgggtt attcacttca tgctgccggt
    23821 gtcatacttt ccggcatttc tttgaaggga ctgatcccca tcgatttcag tgaagatcta
    23881 cctgtttctc tttgggatat ggattctctt cgaatagttg gtctgcagaa aattgatatc
    23941 ttgggcttga gaaatctctc tcttttgaag gaaatgacac aaggtaagga accctgggaa
    24001 agtccggcat gcgattcaaa tacctatgag acattgggaa gcggttatac taccggtatc
    24061 tttcagctgg aagcacctta cgctacgaaa attattagat ccgtaaagcc ctcgtcactg
    24121 agggatcttt ctatatgcat cgcattaaac agaccagggc caattaaatc tggagttaca
    24181 gagcgtttcc ttaagctttc gagagttccc tctgaaatcg agaagatgag gaaaagagtt
    24241 cccgttcttg ctgagacggc agggcttctc gtttatcagg aacaagtcct aaagatcgct
    24301 tcgagcttac tatctcttga agcggatgac ggggagcttc tcagaagagc tctttcgaag
    24361 aaagataggg atgccgttga caggttgttt aagagctcgc cggggtacag cagacttcca
    24421 gatgaaagaa gagcgaagtt gtattcgttc cttcttgatt ttgccggtta tgccttcaac
    24481 aaatctcata gtctaagcta cgctgtgatt tccttctggc tagcatattt caagtcaaac
    24541 catccggaga ttttctatcc cctcatgctg cgagaattac cacggacttc ttttggaagg
    24601 ctagtcgccg aaatgaggga caggggactc aaattaagca tagaaaatga agttgagggc
    24661 cgtaaaattg tatcgctttc tattccacaa ctgctgaaga aaggtgaact gaagccaaaa
    24721 ccatttgagg attccttctt cacgttcgta aggaacaaca gatcaaagta tcaggcaaga
    24781 gaccttgaga gactcataaa gagtggctac ctggattgct atggcagcag aaacgatctt
    24841 ctgaagaaaa tgaatgacgc cctcacgggt gtgaaccccg aactgaagtc aatacgatct
    24901 gtgttcggtt ataaagaaga gcttcaagag gcaagagagg aagacacccc tgttgagaga
    24961 gccatgatgg agattgagac tcttggattc aatgtcacag agattcagcg accggatatt
    25021 tcatcggaag ttactgactt tgaaataaca agcgccgttg cctctctgag aacagggata
    25081 tcgccctaca agagaatcga ttatcttgga aagagtttcg ttactgacgg aaggaccttt
    25141 attcagatag aaagccacgt tcctcttcaa ggtttcgtct tgttcaataa tggaaatcct
    25201 attgaaatga aggagagaat tttagaagtg aacaggattt ttcatggcca gatcgatcgg
    25261 tcattcttag cagtcggctc caagagagaa aacgtagttg tcaaagttag gggtaccgaa
    25321 aaaatcatac ctggggcaag gccaatgggt gtagatgccg ataagataat ctggagataa
    25381 ttggggagga agcaatgtac atttattttg taaggcatgg agaaacagaa tggaacaata
    25441 gtaaccgctg gcagggaagg tccgatattc ccttatctga aaagggaaga aagcaagctg
    25501 aaataactgg cacatttctt aaagaacacg ttcccagtgt agcggcaatc tattccagcg
    25561 acttgaaaag ggcaaaggaa accgccgaga taattgctgt ttcatatgct gagacaccag
    25621 ttgctaaccc ggtactccgc gaagcggatg taggactctg gaatggcctt gagataagtg
    25681 aagcattgaa gtgctatggc aatctgatag agaaatggag gaaggatcca tgggcagaca
    25741 ttccgggaac tgaacctctg ggggaagtgc agagaagagc ggcgagtttc atcagatatc
    25801 tctctggaaa ttttcagggg aaacatgtga ttgtggtttc gcatgccttg ttaatcagaa
    25861 ccgcaatttg ctacgcagca ggcctccctc tggaaaatca ctacagactt tccttacaca
    25921 actgctcgat ttccacaatt aagattgaag gagcagaaat gagactgcta gagctcaatc
    25981 tgtggcgcca tatggaggag aactaacgta tgttacttag ggtgaatctt cttccacgga
    26041 caattgatga gagcacggat ctcgctgtag taattgatgt tctgagagcc agtagtacta
    26101 taacgactgc tcttcaacta ggcgcagaga aagtcattcc ggtttctaat gtcaaacagg
    26161 cgctggaaga aagaaatagg aatcccgagg tagttctagt gggggagagg gaagcattga
    26221 aaataccggg ttttgatcta ggaaattcgc cggttgagct ttctgaaagt tttgtgaagg
    26281 gaaaggaagt tgttatgaca actactaatg gaactgtagc tgctctaaaa tcaagaaaag
    26341 caaagaggat cacagttgct tcattcctta atttggaaag cattgccaga cacatagaaa
    26401 gagagaaagg aagagttgaa atccaatgcg caggtagtga cggagagcct tcgctggagg
    26461 acacactgct tgcgggggcg cttgtcaaac gcttgggaga tgtcggtata aacgatagtt
    26521 gctggatagc ctcttttctt tatgaatcgg ttaggatcga tttgaaagac tttattgctg
    26581 aaaacggagt acacgcaaaa aaacttctgt ctcttggctt tagtgaagat ttatcctttt
    26641 gtgctaaaat caacttgtac gatctggttc ctttgtggcg gaacaacggt ttcgtacggg
    26701 ggtaattcaa tggagttcgt ttctaatgct ttttttattc tagctatggg cgcgcttttt
    26761 ctgtcattga tcttttttga gattgggaca aagaaggtgc gaaagccaaa gagcgaagtg
    26821 aagcctgaag attacaaacc ttacgatagg aaaggctggt attcccttct ggcagcggga
    26881 gggtttcttg gtttgtcctt gttatttgct ttgatcttat aaggtcgagg gggttgaaaa
    26941 aatgcctaac aagctagcag ttgacaacgt tgtatctaaa ctgcctgggg ttcatagtgc
    27001 aagaattgtc tatgatgaaa gcggagaaat ggtcgagatt catgtattgg cagatattac
    27061 caagcctgca aagcaactgg tcagagatat tgagacagct atttatgcct caaccggaat
    27121 caagatcgat agaaagatag tatctatagc gcagatatct cttgattcta acgacgagga
    27181 tgaagcttca gtagtaagac cttaccagaa ggaagaactt caagggtaca actttcagct
    27241 aacaagcctt gaaactaggg ccacgacaag gcgtcttgat attactgtgg ttctagacta
    27301 cgatggcaaa gaacttgaag gttcttcgat tgtcgatgtt ggagatgacg agaaatatat
    27361 ggcgatcgtt gagtcgactg tggctgccat tagagaggtt ataccaagtt tccggatcga
    27421 gttcatcgaa aagctcgagt acggtatgac agaaattgtt atggcagtcg gatcttcgtt
    27481 ggtaaatgga aagagaaaaa gagaggctgg cgcgagacta tgcaagaaag acagcctgaa
    27541 tgactttgct ttggttgtct tagaaataat aaacaagata tagattctca ctaaagagaa
    27601 ttatctattt cggaggtgct ccaatggatc tgtcgcactt tgtctcttcc gtaacaccat
    27661 cggccactct agagttcaat aagaaggccc ttgagctttc taaggcggga gagaacgttg
    27721 tcaaattcac tgccggcgaa cctgattttc ctactccacg tcccatagtt gaggcggcca
    27781 ttactgctct caacgaggga aaaactaaat acaccaatgc ttccggcata gatgaattaa
    27841 gaaaagcaat cgctacgaag atgctgaagg agaacggctt aagctacact cctgatgaaa
    27901 ttgttgtgtc aaacggtgga aagcaagcaa tctataatgt acttaaggcc ttgctgaacg
    27961 tggatgacga agtgataatt atctcgcctg cctgggtgag ctacgaagca cagattctgc
    28021 tctgcggtgg tcgtccagtg gttgttccat ctgagcttga taagggcttt gttccagaga
    28081 tttcgaagat cgcagaggtt ataagcgcaa ggacacgagc cataataatc aactctccaa
    28141 acaacccaac aggagccata tatcctgaac acttccttcg agatcttgcg gtcttaagca
    28201 aggagagcga cctccttgtc atttcggatg aggtttatga aaagctggta tatgatgctg
    28261 cacatttttc cattgcttcg attgatggga tgaaagaaag gacggcggta ataaatgcct
    28321 tctccaaaac ctatgccatg acgggttgga ggataggtta ttcggcaact tcacttagac
    28381 tggccaaggc tatctccaag attcaaagtc atcttacatc caatgtcaat actataactc
    28441 agtacgcagc actgaaggca tttgaagttg acacttcttc aatggttgaa gagtttcgag
    28501 ccaggaggga cttcgtgttc tcaaaactga aaaaaatcgg tctgagattt cacaaaccgg
    28561 cgggcgcttt ctactatttc attgatataa ggccatttct cggcggcagg ttcaagaatt
    28621 caaacgattt tgctatagcc ctccttgaag aagaaaaagt gggaatggtt cctggaagcg
    28681 cattcagcta tgaggggttc ataagaatgt ccttctccag ttctttggag gaactcgacg
    28741 aaggactaaa cagactggaa aactatctca gaaaacttta attagcgacc tgtccagtca
    28801 aggctccatc tgaaggattt agtcaagaac tgaacgcttt gaagatcgga tcttgagaga
    28861 tcatctagca ttctattcat tgcatctctg aaggcatact gtgtattacc agagcgtatt
    28921 tccatgagac actcaactgt tgcggcaaat tgatcggcag cccaaacaat tttgccttcc
    28981 gctgattcaa agggattcag cattctggat tttagttctt tgataagttc atctggcatt
    29041 ccgtctagga ggttctcggt aaccatctgt tcttcaacct gagcaatcac ttcctcgaag
    29101 cctggaacct tctttttcgt cggcgtgatt atatctccag tcatgctttc gggaatgtca
    29161 tgaagcagag cgcgtttgac agattcaaca gggtttacac tgtttccttg ttcttcctca
    29221 atacatgaga gtatatatgc gattattgtt acgtagaaag aatgagcggc gacagtggtt
    29281 gggacattcc tgttgagcct gttccagcga atggctctca gaagaaccat taaggcgttt
    29341 gtgtatcggc tgagccagtt gttgcccgtt atcagacgat atggaccgaa ttcctcgaaa
    29401 tctttgatcc tagcctcaag gtccttcaga ggtctttcaa agaactccgg aaagcttctg
    29461 gcatgaagac cggcttccag tttggccgat agaagatctc cagtctggat aatctttcct
    29521 tcgaccgaat tatctttgga gtgcaccatt gaaaaaaaga aggcatcgtg tctctcatca
    29581 ggcaagtatt gaataatctc ttccactgtc ttttcaaaga catcgttcca cttgtcaggt
    29641 gaaagggatt ttattagaat tttagtatca accgagatgt ctgaaagcaa gcatttaggc
    29701 agttctttta agacgagtct ctggaagagc tccttttggt cgatttcgaa acctttcaaa
    29761 attgccattt cagacattag caggcagtac tgggcagaaa tgaaggcatt ttcggcctcg
    29821 ttggttctta gaagagtcgg tgtgttgttc cacctgtaca ttgtgaagag attcgttgcc
    29881 agttccagaa gtttcacata tccttgaaac acggaaacac ctcctgagta cgttcatgaa
    29941 tctatgttgt tacaattata attgttaggc ttatcaaata aaaggcttaa cgatggaaag
    30001 aggcggtttt atgaatattg agaagtggat taacagaacc tacccggttt tcgaggaaag
    30061 ttcaactctt gaggatgtct tcagtgtcaa agcattagcg ggactcacaa acataatctc
    30121 cgttgataaa gaaggaagac tgtctgggac tgcaaggttg gatgcgatcg atgactttga
    30181 tctttcaaga aaactctctg aaattgttgt tgagccggtg ttttactgcc gtgactccga
    30241 tttcatagaa gatgccgcgc taatgcttat tgagagtcat gactatgtcc ttccagtagt
    30301 caatgacgat tttgtcgtcg taggggcaat tggtgttttc gaaattttgg aaggtttaat
    30361 ggaatttact gctatggata gacccggtgc cagaatctcg ataattctcg aggacaagcc
    30421 gggcgctctg agagatgtag ttgattttct tgcagaaagg gaggttaaca ttctctctat
    30481 tatcacttcg accgcagaaa gtaaggaatt caagcgagtt gtaataagaa cagatgagtc
    30541 agacataagc ttgatcgctg acattcttgg ggaaaagggg atctcttttg aaagcataag
    30601 cgaagaggag ggtttcagtg tctgaaacac taagctatct aattctaggc gcagtacaag
    30661 gtgctacgga atttcttccc gtttcgtcgt ctggtcatct aacaatcttc tccagttttc
    30721 tcaaagttcc tcttgattca gaatcaaaag cagcattttt tgcggtgctt catctggcaa
    30781 ctttctttgc cgttttgttg ttcacattta aagatatatg gggaatactc acaggcctat
    30841 ctaaacctga aaagaggaaa atctcattca gattcattgt gctgcttctt gttgcaacta
    30901 ttcctgcagt tctagttggt ttcctcctag aggatgaaat caaatctctc ttctctgatg
    30961 tggcatttgc ttcgataatg ctttttgctg cagcggctgc tttattcatt tcggatagct
    31021 tgaagggtga acgtaaaatc cttgatcttt caattgcagg cgcgctttca ataggcattt
    31081 ttcaggcggc tgcaatagct cctggactat caagaagtgg cttgacgata ttcggggcat
    31141 tacttatagg aatgactaga agtgacgcag ttagattctc ctttcttttg agtcttccag
    31201 ttacctttgg tgccggtatt ttggaactct ccaaagtaag tcttcctata tcaaccgtcc
    31261 ttttgtcatg tcttcttgcc ttttcaactg gtattgttgg gctttggctc ctcaagaaac
    31321 ttgttctgaa gcgacgtctg agattctttg gcttttactg tataattata gggattgtag
    31381 gtctgatttt tcgaggagtg atgtgatttg gatttgaatc tcctctttcc cggctcctta
    31441 gtgtacgtgc cgggaatgat ggatgcatcc acaagtagct gtgttctgct tgtggaagat
    31501 tacaagaaaa ttctcatcga tccaggcggg ttcgcttcga tgaaagtttt ggaagacgga
    31561 ttaacttctc tgggtctctc cccagacgat ataaccgata tattgctcac tcattttcat
    31621 cttgatcatg ctttcaacag tgtttttttc tctaaggcga cgatacattt acatgaaagc
    31681 ttctccacta gagattacag ttcatttggt ccaataattg gatctgtgta tagtaagatc
    31741 attgactcct ggaaatgtat agagaccttc tcgggtggcg aaaggatttt gggagcaata
    31801 gatgttctca gttcgccata tcattcaaga gatcatgtgt ccttctttct ccaaacggaa
    31861 aatcatggaa gggttttcat ttgtggagac gtctgcgcaa gacagataca ttatcacgaa
    31921 atgcgaaaag gaatgagatc agatccagcc gctgcttttg tcttgaaaca ctttgagaga
    31981 gcagatgctg ttattttctc tcatgacctc ccgctgttca agaggtgatt gaatgaaggt
    32041 gctttttgta tcttacgagg tttatcctat agcgaaggta gggggtcttg ccgatgttgc
    32101 aggatccctt ccgaagtatc ttaaagagct cggagtcgaa attgatttgt taatgccatt
    32161 tcataagtcg atagacaaaa atatggctat caaaacggat gagaacgtca gtaccaggtt
    32221 tgtcaaaaga gaggtttctt ttgaggttta cagagcgaag ttgccgaaga gcgatgtgaa
    32281 cgtttttttg ctttataacg aaagacttat ggactcaaag gagatctatg gtgcatcgga
    32341 tcttggtttt caggcaatga gcttttccga tgccgccgcc ggatttgcag ttgacaaagg
    32401 ttacgatatc gtccatctca acgattggca aaccgctttg atcgcagtat atcttaaaca
    32461 gtgtgaagca gagtgcaaga cccttctttc tattcacaat ctcgcctatc aaggtgtata
    32521 cggtaaggaa tatctagatc tgtcaggaat cgaaaaaagc aattgggacg aaatctctga
    32581 ggcaggaaaa ataaattttc taaaggctgg gctcgttttt tcagataagc ttagcactgt
    32641 atctccaacc tacgcaaaag aaatccagac gaaagaatat ggtgccggcc ttgaaaaaat
    32701 cctttctagg aggcggagcg acctggttgg aatcttgaac gggatcgatt actacgaata
    32761 tgatcccgaa agagatgaac gactttgggc aaacttttcg caggacaata ttgagggcaa
    32821 gaaattcaat aaatctaatt tacagcaact tgtgggtctt ccgaaaagag atgatgcttt
    32881 gattggactg atttcacgtc ttgtagatca aaaaggactt gatttgattg aagatatcaa
    32941 ggacgagttg atgaagcttc ccattcaaat tgtagttttg gggacgggag agagcaagta
    33001 tgaagatatg ttcaggatac ttcaaaagaa ttatccggaa aaggtggctg cgagattgac
    33061 ctttgatctc gatcttgccc aaaaaatcta cggtggagca gatatgtttt tgatgccctc
    33121 tagatatgaa ccatgtggac ttggacaaat gtttgcgatg agatacggga ctgttccaat
    33181 tgtcagatac actggaggac tcgcggacac tgttcatgaa tttgatcctg aaaacaatgg
    33241 taatggcttt ggattcgtag agtacgatgc gaaaaagctg cttggagctt tgaagaaagc
    33301 tctcaaagtt tactttcaac cagaactttg gaagaaagta gttagaaatg cgatgagaca
    33361 ggacttctca tggagagtct cggcaatgaa ttacctctcc ctgtataaga agattctgga
    33421 ggttaataac gatgcctgaa cttagaaagg atccgataat caagaggtgg gtgattattg
    33481 cgaccgaaag agctcgacga cctcatgatt tcataaatgc taaagaaaat gttgaatcag
    33541 ccttttgtcc gtttgactat ggcaatgaac acaccacacc tccagaaatc atggcgttta
    33601 ggcccgcaga tactgttaag gacagtccgg gctggtgggt gagagttgtg caaaacaagt
    33661 ttcctgcatt ggattcaaat attgagcccg agcgattcgg tcacggcatg tacgatgtca
    33721 taaaggggtt cgggacccac gaagtcatca ttgagacacc ggatcacaac gcttcaatgg
    33781 cgactctatc gtatggacag gttagagaag tagtttgggc atacaaagaa cgtctccaga
    33841 cactcgaaaa ggacaacagg ataaagtaca tcttgatatt caagaatcat ggaagagaag
    33901 ctggcgcctc gcttgtccat tcgcatagtc agctcattgc tacaccaata gttccaaaaa
    33961 gagtcgcaga agagatagct ggtgcagctg agtattatcg cttcagagag agatgtgtat
    34021 attgtgatat ggtgaatcaa gagactcttg aagggactcg agtagttgaa gaaaacagcg
    34081 atttcgtagc gtttgaacca ttcgctgcga ggttcccctt tgagacatgg attgttccga
    34141 aggttcatag tcacaatttt ggagagatat ctgctactca aatcgagagt ttttcgataa
    34201 ttctcaagaa tacgttgcta agaatctaca aagctctaga taatccaccg tataacttca
    34261 tgctgcacac aggaataaga ggaattgacg aatcgaatca ctaccactgg catctggaaa
    34321 tagtaccaag gcttacaagg gttgccggat ttgaatgggg atcgggtttc tacataaacc
    34381 ctatgcctcc agagcatgca gccatgtatc ttcgtgaagt cagcgttgag gaggaatgaa
    34441 aatggaaacg aaagtaagcg atgaactcct taaacatctt gaaggccttg caaagcttga
    34501 actacttgaa gacgagcgta ttgaactgaa aaaggatatt gaggagatac tgcagtatat
    34561 gacgcttcta gatggagtag atcctgacgc cgaagaaatg gtcactcctg ttgagaagag
    34621 cctcgagcct agggaagacg agttggttga gttcaaggaa tcgagactca ttcgagatct
    34681 ctttcccgat acagagggga gctatttgaa ggtgccaagg atttataaag aagagaaatg
    34741 agcaatgagc aaggaggctg gtaagagata cgaggatctc gcatgcgaat acttgaaaga
    34801 aaacggatac aggataatag caaggaatgt tgcgtataga ttcggggaaa tagatatcgt
    34861 tgctcttgaa ggcaggattc tggtgtttgt tgaagtaaag gggggaagct cgttagtact
    34921 cccgagatat cgtgtagatg aaaggaaaat taggagattg gaattggctg cccagagata
    34981 tattcttact gaagagccta agtttgagga agctagactg gatgtcattg agattctaga
    35041 aagcggagca gttaatcatt ttagatctgt tggaaggtgg tagagtgctt agaggtgaac
    35101 gagcagttaa tcaggagtac aagagatggc tagataatgc atctgatgag atcagagatg
    35161 aactgaagga tctcactcct gaagaagtta gagagagatt ttcactggat ctcgaatttg
    35221 gaacaggtgg aatgcgtggt gtaatgggtg caggtacaaa taggataaat tcttacacca
    35281 tcaagcgcgc ctctttaggg tttgggcgct ggatttcaga gaattacagt gatccatctg
    35341 ttgtaatagc gtttgatacc agacataaat ctgcgcagtt tgcccaaacg acagccgagg
    35401 ttctttcgtc catgggaatt agagtgcacg tgttcatcga acccatgccg gttccaatac
    35461 ttagttatgc agtgagagag ttgaaagcta gcggtggtgt cgtgataacg gccagtcaca
    35521 atccaccgca atacaacggt tttaaggtct acacttccga tgggacccag gccgtacccg
    35581 gctatgcaga agagataata aaaagagtcg aagcatcgga ctattttgag gatttcagtc
    35641 caagaagtga actgataaat agcatcccga attcccttct tgagaaatac atcgacaccg
    35701 tgaaagaaaa catactagga cttacttctg actatgaaga actgaatgtt gtttacacac
    35761 ccctacatgg gagtggaaac tttcccgttt acaaagctct tacagtgctc ggacacagag
    35821 tgaaacgagt tgaggagcag gctataccgg atggagattt cccgacggtg cagagcccaa
    35881 atcccgaaga tccaagagcc ttttccatgg ctctggatgt tgcgagagag ataggcgcag
    35941 atcttgtaat agcaacggac cccgattgcg ataggctggg gataatggtg gcacacaatg
    36001 gtgattatgc cccactaaac ggaaatcaga ccggcgcaat aatgacttcc ttcatactgg
    36061 agcgaatgaa agattcactg ccgtcagatc cgttcattgt aaaaacgatc gtctcgactg
    36121 atcttgtgaa gaggatcgca tccaaatatg gtgttgaagt caaagagact cttaccgggt
    36181 tcaagtttat tggagaaata atcgaaaaag ctgaaatgga tggaaaaggg aactatcttt
    36241 ttggatttga agagagctac ggctcgctgt ttggaaaaca tgcaagagac aaagatgcgg
    36301 tcagcgcggc cgcactagcc tgcgcgatcg gcggatacct taaacgcaaa gagatgacat
    36361 tgatcgacta tcttgaagaa atctatagcg agtttggcta cttccaagag gcacttgtga
    36421 acaaagagta tgaagggatt caagggaagg aaaagattga gtcaataatg acaggactca
    36481 ggcatattga gccatcgaag ttaaaagaga tggccatcag agaaatgagg gattatctga
    36541 atggttatgg taaccttcct ccttcagatg taatatcaat ggaaatggag aacgactgca
    36601 aaataatagt gcgaccctca ggaacagagc caaaaatcaa gttctacctt atggccaggg
    36661 gagaaaatag agaagaagca gagaagaaga tcgaagaaat gaaagaactg gttgaaagga
    36721 ttgtatgctt atgaacttca ggtacgcgca cattctttat aggggagatg acttccttac
    36781 accaaaaaga gctacttttg atcacagaac aaaaactggt ttcatgacca cgtggtctac
    36841 tgatcattca gctgagttgc ttaaacataa atattggaat tgccttagtg cttatggcct
    36901 tgagagcgcg atgaggcgga agatcacttt cgaaagaaag cacagcgact cgaatctgct
    36961 gtactttaag tatgaactag aagttccaga agctcttgaa ggttatttcg atcagacaac
    37021 gtttgaggtc atttcaaaga atttgtcgat tagggaaact accgaagaag tttacaagat
    37081 gctaaaggac tacgaagaag gatcgcttag cttcaatgac caaaggttct catcatggct
    37141 tgccatgaaa actggagaac tacagttgag tgtaaacgaa aaaaaagagc ttgagaaatc
    37201 actaataaag tatgtagaaa ccattgtagg aagaattctt tggacagttt ataatggaga
    37261 tctaccaaaa atggggaagg acctttcctc tatcgtttat ctttacactg aaatgcttga
    37321 cttgatagct ctaaaatgat tatgaagaga ttcgctgcta tagtatctta cgatggaaca
    37381 gattttttcg gtttccagaa ccagccggga ctacggacgg ttcagggcaa gtttgaagaa
    37441 gcgcttcata gaatccataa attttgcatc tctagtcaag gcgcagggcg taccgatact
    37501 ggtgttcacg ggttcggaca ggtaattgcc ttcgattcta atctggatcg cctggaagca
    37561 actaccatga gagacgcatt gaacgcaaat cttccatctg acatgtatgt caggaaagta
    37621 catgaagtcg atgagaattt cagcccaaga ttcgccgcaa agaaaaggat ataccactat
    37681 tacatacaaa cttcgagtga accggatatt tttagaagaa ggtttgtctg gtggtttccc
    37741 tattctctcg atattaaatc aatgagaaga gcggcagtca atctcctggg cgaacatgat
    37801 tttgctgcat tcaggaccgg caaggacgat agaaatcctg ttagaactat tacggcgata
    37861 agaataataa gatacaaacc ttacataata cttataaggg ttgaaggcat ttcgttccta
    37921 aagagaatgg tgagaaacat tgttggaacg cttgtaaaag tcggaacagg aagtgtttca
    37981 gaagaatcgg tatctgagtt tctttcttct catgacagat caacattacc gggaacggct
    38041 ccacctcagg gactcgtatt ttacaaagtt atctttgatg aattcgagac ctagatttga
    38101 tgaacaattg tttgaactag tgctgtctga aaggagaaca ggatcatcaa ttttactgtg
    38161 aagcggaaaa ttcattatta atggtatgat tgttcttaaa ccgggctgca aggtaatttc
    38221 tgcgtaccgg atttttgtgg gaagacttga aaggcgagtg cgtcgattaa gtccaaaatg
    38281 aaagtggtag caatcggtag aattggtacc tatactggag agcaagataa atcgggtagc
    38341 tcctcacata atggtttcaa tcagctaatg cttgatagtg ccacatctac cttagtctat
    38401 cttattgtgg gcggagaaag atcagccatt ggaaggagag gagattcacc tggatctaca
    38461 aaatcagaaa acttgcgctt gtcgatgctc aagagaagaa ggttcaacag ctggatcggc
    38521 ggaactgcaa gaagttttct gggcgacaga agtgaagctt caatctacta aaggatccgt
    38581 ttgaagctgt ttccggactg agaaaaaagt aattggttga gggagtcgct attttgtatt
    38641 tccgaaatta gccaaaggat ttgatccttg ctaatcagta cgaagaaagt aaatttgagt
    38701 atctgcttca tacagtaatc agaaacaaac tcagtggcga ttctaaccgg tggccgaaga
    38761 gtcgaaagta ttttgagaag aatgtaatca tcagatttga agggagggag aaccccgatt
    38821 tgacggttag gtgatcggga aaggtttgaa atccttatta gttggtttgt tactgattct
    38881 ttctttttcc ggaataatgg cccaggagat ctttgatttc gtgcctgaag atgttggtta
    38941 ttttcttata cttcgaggat tgagatcaac aatggaggct cttagggata acacgaattt
    39001 ttttggagcc tatctcggag ataatggata cggagccgaa agagcttttt atggaataat
    39061 cgatgcggca ggatacaacg ctgaagtcga taccagcttc cttaagtcag cgttgaacaa
    39121 tgacatacta cttgccgggg aaggaatcga tctttcactt gacgacctgt tgatgttcga
    39181 tcctttttac cttcttgaga aactaaagct tactgagaac agagttttca tggcttggag
    39241 aacggacagt tccttcgaat tgttgaaggc ggtggcggcc cttcttgata tgtcgatatt
    39301 tgttcagccc ggcgaaagcg tcaatgaact cagaagcaac gctggaacgc ttttctataa
    39361 tgccggcgac gattacctga taatcggtgg aaacaaagat gcagttacac ttgccctgaa
    39421 aacctatgaa ggtgaaggta aaaggcttct agaatctaac ggcaaaggaa gggaagttgc
    39481 tgaaatgagc tatgattacg ctttggccgg ttacattgat ggagaccgtt ttcatctaga
    39541 tctggggctg gaatcaactt tctcgattga aagcactatc ttatttgcta ggccagaagg
    39601 acacacatta accgcaacaa tagttcagga tactgttttt ctcagcagtg aagatattca
    39661 aaagatgctc tctgttactg acacaaaaga actggaggcc tccaaagaga cttttgggga
    39721 ttacaccgta ttctttccgt cttcatccgt tgaaactctg agaaaggagc ttgcgcactg
    39781 gtttgaactt gatttagagc cctatagaga gctcgctgat tttgttgtac ttctttctaa
    39841 ggagagtata ggaaacgcaa gaatatatgg agatctcgtt tcagaaacac ccagagtgac
    39901 agtcgtattt acgaacaaaa gttcagacac tgagaaattc gagaattctt tgaaagagtg
    39961 gggtgctttg cagtcagcac ataaggacag taggatctac gagcttgaga acgattttga
    40021 cacaatttac tttatttttt ccaacgaaac taccgtattg acttcccttt cgcccgatga
    40081 atactacaga ttgctttccg attcagaaag tctcagtgat aacattagct acgagtttgt
    40141 acgaaatctt ggcctagaaa aagacatagc acacgctttc atcgacatga aaaaagtctt
    40201 caagtctttg atgggagttt ccgtagacag tgctctcttc taccagcaga catttgatga
    40261 aaacggcaat atgattcacg ctctccgctt ctattgaggt gatttaatgg gattgttatc
    40321 cagaatattt gacaagaata atatgcttct caaaaagtac tccaaggtgg ttgaaagcat
    40381 aaacagcttg gaaaaaaccg tgagtaggta tgaagaagtt gactttcaaa aaaagaccga
    40441 ggagttcaaa gctagagtct ccgagggcga aacattagat tcatttcttc cggaagcctt
    40501 tgcgctggct agagaagctg ctaagagaac ggtcggtatg cggccattcg atgtgcaact
    40561 aatgggagct ctcgcgcttg acgaaggtaa aattgctgag atgaagactg gagagggaaa
    40621 gacactggtg gcaacaatgc ctctctatct taatgccttg tctggaaggg gctgccatct
    40681 tgcaaccgtg aatgactatc tagcaaagag agatgctggt tggatggggc cggtttatga
    40741 gtatcttggg ttgaaagctg gattcattca ggcggcgatg gaaccttctc aaagaaaaga
    40801 ggcgtacggt tgcgacataa catatggtac tgctaacgaa ttcggattcg actatcttag
    40861 agacaatctt gtgtattccc tggagaacaa ggttcagagg ggacacaact atgtaatagt
    40921 tgacgaggcc gattcaatcc tgatagacga agcaagaacg cccctcatta tttccggccc
    40981 cgctcaagac tcatccaatc tctataggca gtttgcattt tttgccaaac gttttgtagc
    41041 ggaaaaggac tttgttgttg atgaaaaaga cagaacagta actctcacag aagagggaat
    41101 agcaaaggct gagaaactgc tgcagatcga caatctttat gatccaagca attacgacta
    41161 cattttccat ctgttgaatg ctttgaaagc aatgaatcta tataaaaggg aagtcgacta
    41221 cctggtaact caggagggag aagtcgtaat tgttgatgaa ttcaccggaa ggttgttgcc
    41281 gggaagaagg tattccgaag gtttgcatca ggcaatagag gcgaaagaaa atgtgcaaat
    41341 tagacaagaa tcggtgacgt ttgccacaat aacctttcag aactacttca agctgtattc
    41401 gaaggtgtct ggaatgaccg gtactgccgc aactgaagaa tctgagttca tctccatgta
    41461 caacacacca gttgcgatca ttcctacaaa caagaaggta attcgagagg acaaagaaga
    41521 ttcgatttat aaaacaaggg aagaaaagaa tgaggcaatt atcaatgaga tcgctgaacg
    41581 ctatgaaaaa ggacagcccg ttcttgttgg tactacttcg atagagaaga gcgagctctt
    41641 gagcaagctg ctgcaaaaaa aaggcgtacc ccacgaagtt ttgaatgcca agtatcatga
    41701 aagggaagca gagattgttg caaaggccgg tgagcgaaag acagttacaa ttgcaacaaa
    41761 catggcggga agaggaacgg atattaagct tggtgaggga gtagtagatc ttggggggct
    41821 ttatgttcta ggtaccgaga ggcacgagag caggagaatt gacaaccaac ttgtaggtag
    41881 atcgggcaga cagggggatc cgggggagtc aaaattcttc ctttcaactg aagacgatct
    41941 cttgcgcctt ttcggtggcg aacgcatgca gtcgattatg aatacactaa agattgaaaa
    42001 gggacaacca atcgaacacc cgcttctaag caggataatt tcatctgcgc agaagaagat
    42061 cgaaggaatg cattttgaaa taagaaagcg actttacgag cttgattctg ttattgacca
    42121 gcagagaagc gcaatatatg ctcatagaaa ttgggtgctc aaaggtgagg aaatcgactc
    42181 taatgtatta gaaattatcg aagatgttgt agaaagaaga ttgtcgggtc tcgatcgtct
    42241 accttctttt gatgaaataa aggtctcttt tggcttcttg ccggcaggag acattgagag
    42301 tcttaaaaac ctgaagagtg tggaagatct caggagtaaa accctttcag tgcttcacga
    42361 tatttatgag gaaaagaagt ctgctttcgg cgatgagttt cctcaggtca tcaagtacat
    42421 tatgctacgg atgattgatg aaagatggcg aaggcatcta gaagctatag agcacctaaa
    42481 agactccgtg ggtctaaggg cttatggtca gaaagatcct gtgttagaat tcaaaaagga
    42541 atctttcata ctattccagc agttgaccga ttctctatac gatgacatag caagcgctat
    42601 cgtaagaatt gtaagagtcg atagcgataa ggctaagcag aacgccgata aggagtttaa
    42661 gagtctgcag gccgttcatt ctgatttcag cggaggcgat aagaagaaag aagttgctgg
    42721 aaaaaagaag ggcactaaaa gattcaaagt taagcgttga accggaggtg taatttgata
    42781 agttatgaaa ctaactcgag aatacaagag ttgaaggaga aatttgagtc catcagggct
    42841 acgcttgatc tcgagcggat agatgaaaaa ctaaaggaaa tcgagaagag gatgagcgac
    42901 ccctctattt ggaacgataa gcgtgaagcg tcgaatctgg gaagagaagc gcagtcttat
    42961 cgatctacaa tcgaacttct gagagatgtg gaagaggaat ttgagagtat tgagattgca
    43021 gtagaacttt caggagaaga tgagagttat gtaaagcatg ttgaagaact tctcaggtca
    43081 gcggaaaaaa aggtaagaga attcgaactt acgattcttc tgagtggaga gtatgacatc
    43141 aacaattgct tcatttcaat tcaccccggc gccggaggga cggagtctca ggactgggcc
    43201 tccatgttaa tgagaatgta catgagatgg gctgaggcta acagatacaa aataacgatc
    43261 gtcgatgagc tacctggaga agaagcgggt atcaagagtg tgacaattaa tctagctggg
    43321 ccgcatgctt atggaaagat gaagtttgag gcgggagtac ataggttagt aaggatttcg
    43381 ccattcgacg ccaatcacag aaggcacact tcctttgcgt ccgtaagtgt ctttcccgag
    43441 atggatgaag ttccagagat tgaaatcagg cccgaggatc tgaagataga cacgtacaga
    43501 tcaggtggtg cgggcggtca acatgttaat aaaacggatt ctgcggtcag gattactcat
    43561 ctaccaaccg gaatagttgt tgcatgtcag actgaaagat cgcagcatca aaacaaagcg
    43621 aatgcaatga agatgttata tgcaaagctt ttcgagattg agattgagaa aaaaagaagc
    43681 gaaaaactaa aattgatggg agaccagaaa gagatttcat gggggaatca gattagatct
    43741 tatgtctttc agccttatac attggtgaag gatcacagaa ctgattttga gacgggtgat
    43801 attcaatctg tcatgaatgg cgagatagat gaattcattg agaaagaatt gctcttcttc
    43861 tcatcaattg agaaatcctt ggaatgattt acaccgttaa tttgactttg atggaagaaa
    43921 aatgttatta ttctcttgag cggagtgaat ttagtgtcca ggcagtacga tgaacgccac
    43981 aggagcgtga tgattcacga agcgctccat tatttgcttg gtgggcgttt aactggcact
    44041 tatgttgatt gtaccgctgg tgaaggtggc catatcggag cagtgcttga agccaccaaa
    44101 ggtcgggcaa aaatcatcgg acttgatgtt gatagtgagg ttctggatat agcggagcag
    44161 aatctcaaag attaccggga aagcgttgaa ttattcaatt tatcttatgt cgacttcgaa
    44221 attgcattga aacaggctgg tgttgagaaa gtggatggtt ttctcctgga tgttggagtt
    44281 tccactttcc agttgaaagc aaagggtagg ggcttctcgt ttgaactgga tgaaccgctc
    44341 gatatgcgta tgaacctctc tcagaaaaga acggcagcag atgttgtgaa cagctacaag
    44401 gaagatgaac ttagcaagat aattttcgag tttggtgatg agaagcgttt tgcaaggaaa
    44461 atagctagaa gcatcgtatc gagaaggcct ctgtttacga cgaaggattt gcacgatgcg
    44521 atcaaggcgg caataccacc ggccgaaaga tacaaacgaa gacgacattt cgcaacaaag
    44581 acttttcagg cagtaaggat tgaagtcaat caggagcttg agaacatcga aagtactttg
    44641 agaaaaatcc ccggttttct gaaccaaggc ggtaggatcg ttattatttc ttttcattct
    44701 ttggaagacg gcatagctaa gagagtgttt agggagaagt cggaatctga gctgaaaata
    44761 cttacgaaga aaccggtgca gccgtcgaaa gaagaagtag aactcaatct aagagcaaga
    44821 agtgcaagat tgagggcagc ggagcggatt tgaagggagg aacatctcgt gcaagccata
    44881 acagcgaagc ggaagccgct ggttcgcgaa ggcggcaaag ccttggcaaa cagcgatgtt
    44941 tggggtttat ttggttttct gcaagtatta gtgatttttc tttcattggc agtctcaatt
    45001 gctctcccgt tctattttgg caacgaggtc gacaaaattt cagaagtggc atcaagtaga
    45061 gaacagagca tctcagtaat gaaatcgcga cttgaagatg tatctaggga aatttcatac
    45121 ttgcaaacgg ttgtaggggc gagagcagaa aagtagaact ttatcgaggt ggatcataaa
    45181 tcaataaact tgctagcagg gtgacgctgt tatatgtgct tttgattctt tgcgtaggag
    45241 tcttcgcagt gagggcagcg tatgtttccc tttttactga acctgaccta cctctggttc
    45301 ttggagtcaa caagataccg gctctaagag gatcgatata cgattccaaa ggtaggttat
    45361 tggccagcga ttcgctgatt tatgaggcat ggctggacct cggatatttg agattgctta
    45421 cttcttccga tcaaatagat agagttctaa aaaatgtaga actttccttt ggaataccct
    45481 tcgaaagact ggtcgaaagc cttcagagca acagaaattt ccttcttctt ggtaccgcag
    45541 ctactaacga tgaaatgcaa cgaaggatca caccgatcac aaagagatat atatcgcttg
    45601 aaatgcagag agaaagactt agtttcagcg aatatggttt ggaaagaatt atcggaaagc
    45661 tagatggagg gggaatcccc ctaaatggaa tagagctgta ttatgacgaa gagctttctg
    45721 ggaaaagaga tggattgatt aggagaactc tgacaagttc gagaagagaa gaaccaacaa
    45781 atggcagtga catctatctc tcaattgaca tagatataca gaaaatggtc tatgacgaac
    45841 tgctagagac tgtggaaaag aatatggccg acggtggatt ggctcttctt gtagagagca
    45901 agactggaaa gattcttgct tatgccggta cctatgactg ggacgttggt ttaatgggga
    45961 tatttgagcc cggttccacc attaaacctc ttatctactc gcttgcatta caaagcggat
    46021 cgataacgga aacaatgaca ttcaattgtg atggaagtat tcaacccgta cctggacttg
    46081 acataataat ccgcgatacc gaaggcgaac gccacggcct tcagaccttt agagaagcga
    46141 ttattaactc gtgtaacgtg gccacggtac aagttggcgg tagaattttg aatactctcg
    46201 gaaaagaaga aatgtatagg caattaacga gaattggatt tgggaaattg tcgggggttg
    46261 accttcccgg cgagacaccg ggaattcttc ccgaggcgaa agattggtcc ctgatatctc
    46321 cttatcaatt tccaatcgga cagggcttag gagtaaacat cttccagatg gttaaggcat
    46381 tgaatgtctt cccagctggt gggcagttta ttactcccac ctttgtcagt gccatacgaa
    46441 atgaagaagg cctgatcaac ataccgaaga aggttgaagg tgatttgttt tcaccgaagc
    46501 ttgtttctac aatgattccc attcttgagg gagtagtcat tgagggcact ggcacaatgg
    46561 ccaaagtcga cggagtaaga atagcgggaa aaacagggac tgctcaaaaa gcgggtccgg
    46621 gtggctacaa tgaagaaagc tactattcat tgttctatgg tttctttcca gtggaagatc
    46681 caacgtatac attatatata atgatagata ctccaaaagc gggggcatac tatggaggtt
    46741 ttgtcgcagc tccaatgttt gcttcagttg taaggaacat ctacggtatt ggagttgaaa
    46801 aggaagtata tgagggcgtt tacagttgga aagcacctga cctgagcggt tattcactgc
    46861 tagatttgaa agagatcgcc gcgatctatg gtttagacaa tattataata catggctcgg
    46921 gcagcgtggt gagtcaaatt ccagaggcag gaaggcccct tggagatcag ttagaagttt
    46981 ggttagggga gctgaactat gatatacgag atgttggggg acagtgaggt actgaaagat
    47041 ttcttcattc ttgaaagagc agaggggtgt ctaacaatta gtgaagacat gcccgacaaa
    47101 gtaaacagat tcagaatggt tgtttcgact tcgggaatgt tcggaagaaa gtcagttagg
    47161 ctttccggat ttgatagctg gaaaaaggaa gagaaaactc agatagagaa actacttcct
    47221 ctctttcaag acgaaatcga tgtcttccta gatggaaaag tgagtccgaa agtaaagagt
    47281 gagaaaaagt tttttgatct tccaaagccc tgggaagaag agaaatggaa tgaccacatc
    47341 atcacgataa gcgaacgtgc aggaatgaag atttctagag ctgcggccga atcgcttttc
    47401 agaaaagttg gaagaagaga atacagaata cttcgagagc ttgaaaagct ttccgttttt
    47461 tcgaatgaaa tagaccagct cacggttgaa agactaattg attttgacaa ggaagtcgaa
    47521 gtcgaatcac tggcgtttga ttttcttaat cacaaagagg actttctaag ctcttcgagg
    47581 aaatgcatga tcccattcgc atattttgcg tcggttctgt tgaatatcat aattgatttg
    47641 ggaacgataa ttgatattaa aaaaggtaag atgaatattt cttggtcaga gataagaaaa
    47701 ctctcaaata ctactagcat tggtacttct cgaattgcca ggcttgttgg atacagtttt
    47761 tccagtacta aagaacaaag aaatgatctt accaggctgt actcagctga aagtctcaaa
    47821 agcctgatag atcttcttca ggaaattgat gagtcaatca aaaatgggaa aatcgatagc
    47881 gaaatcgctc tcttcaaact acatgaagag tcttcaaaac ttcaggtaac tcttgagtaa
    47941 caatttccac ctgaaacatc ccgtttagaa tgcttcaatt gacagtgcca tcgttgcatt
    48001 gtacaattgc tttcggcaaa atgcttagaa ggaggcgaat cgaaaccaga tgcccacaat
    48061 aaatcaattg ataaggcacg gaagaaaaca gttgaagcaa aaatctgctt cgccagctct
    48121 agaaaacaat cctcagaagc gtggcgtttg tgtaagggtc tccacaatga cacccaagaa
    48181 accgaactca gctttgagaa agattgcaag ggtacgtctc tcaaatggga cagaagttac
    48241 tgcctatatt cctggcgagg gtcacaatct tcaggagcat tcggtggttc tcgttagagg
    48301 aggcagagta aaggaccttc ctggtgtaag atacaagatc gtcagaggca cattggatgc
    48361 tgaaggagtg gcaaacagga agcagtcgag aagtcgttat ggtgcaaaga ggccaaagaa
    48421 atgaggaggc agttctagat gagaagacgt cagtctgaga aaagagaagt tccgctcgac
    48481 ccaatttaca acgatgcagt agtaacgaga ttgatcaata agataatgat aggcgggaag
    48541 aaaagcaagg cagaggctac ggtttatgga gctctggagg ttttatccga cagaacgaag
    48601 cagccaccaa tggaagcttt caagaaggct ctgagtaatg taaaacctct tcttgaagtt
    48661 aggccaagga gggttggagg tgcaacgtac cagatacctt ttgaggttcc ggaaagaagg
    48721 gcagtggcac tcgcaataag gtggattgtc acctctgcaa gagcaaaatc tggcaagccc
    48781 ttaaaggaaa agcttgcatt agaattagta gatgcttaca atggtcaggg aaacgccgta
    48841 aagaaaagag aagatgttca caaaatggcg gaggccggga aagcatacgc ccatttcaga
    48901 tggtgaagtt gattcagcag gaggaaagat agtgaaagaa agagctacaa ctctcgaaaa
    48961 gacccgaaac ataggaataa tggcccatat agatgcgggg aaaacaacca cgaccgaacg
    49021 catacttttc tataccggaa aaaattacaa actcggaagt gttgatgaag gaacggctac
    49081 aatggactgg atggaccaag agaaggagag gggcatcact atcacttctg cagctaccac
    49141 agcgttttgg aaggatcatc gcataaatat tattgatacg cccgggcacg ttgactttac
    49201 tgttgaagtt gaaagatcct tacgggtact tgatggggcc gtagcggtat tcgatgctca
    49261 agcaggtgtc gaaccacagt cggagactgt ttggaggcaa gctgataagt atcatgttcc
    49321 tagaatagcc ttcatgaaca agatggacaa gatcggagct gattttttag gtgccatagg
    49381 cacaatggtt acaaagctta aggcaaatcc ggttgcaata cagcttccaa ttggatcgga
    49441 atctggtttc gaaggcatta tcgatttagt gcagatgaaa gccttgcgat ggaccaatca
    49501 agagggaacg gaattcactt attcaaatat tcccgaattt ttgcaagccc aggtggaaga
    49561 ggctcgagag gatctgatta cgcatgttgc tgagttcgat gaagaactca tggatctgta
    49621 tattgagggt gaagaacttc cagtagatag actgaaagcg gcaatcagaa aggcaacgct
    49681 tttgggacaa attacccctg tgctttgtgg ttcggcattc aggaacaagg gaattcagcc
    49741 acttcttgac gcaatagtag actatctgcc gtctccgaaa gacttgcctc cggtggttgg
    49801 cgagatcatc gatagcgaga ggaaaattga agtatatccg gatgagaatg ggccgtttgt
    49861 tgcgatggcc tttaagataa tgatcgatcc ttttgtaggc aaactcacat ttctcagagt
    49921 ttattctggt tcacttgaaa agggagccta cgtcataaac acgaacaaga acttaaaaga
    49981 aagaatttcc agacttcttt tcatgcatgc cgacaaaagg gaagaagttg attatataag
    50041 ggccggagac attgtcgcag tggtaggcct taagaatact atgactggtg agactatcgc
    50101 actttctgag gaaaagattg ttctcgaaaa aatcgaattt cctgagcccg tgatttcgat
    50161 tgcgatagaa ccgcagacaa aagacgatgc ttcgaagctt acaaaggctc ttgttgctct
    50221 agttgaagaa gacccttcgc ttaaaagcta tgttgatgct gagactggtg aaacgattct
    50281 ctccggcatg ggagagctcc atttagaggt aattgtagag cgaataaaaa gggaattcaa
    50341 tgttggctta agagtgggga atcctcaggt agcctatcgt gagacaataa ggtctaaagc
    50401 aataggtgag tcgaaatatg ttaggcagac tggtggaaag ggtcagtatg gacacgttat
    50461 actgagtgta gaacccatta ctgacaagag cgccaatttc gtgtttgaag ataaaactgt
    50521 aggaggaacc ataccaaggg aatttatcaa gccaattgaa cgcggagtaa gagaagctat
    50581 ggattccggc tatctcgccg gttatcctat ggttaatgtg aaggtctcct tgctcgacgg
    50641 ttcataccac gaagtagact cttcggagat tgcatttagc atagccggat ctatggcctt
    50701 caaagaggct gcgagaaagg cgagcccggc tctattggaa ccaattatgg cagtagagat
    50761 aaatactcct gaagaataca tgggagatct gattgccgat ctcaattcta ggagagcaaa
    50821 gattgagggc tttgaaacta gagcaggtct taaaattatc aaagctcacg ttcccctatc
    50881 agaattgttt ggttacgcta ctgtttgcag atctctgtct caaggaagag caatccacgt
    50941 cattcagttc tctcattatt cggaagttcc ggagagaatt gcagaaaaga ttctaggaaa
    51001 ataatgaaga tcccaaatta gtttttggag gagggaaacc atggctaagg aaaaattcga
    51061 acgttctaaa ccccatctta acatcggtac gattggacac attgaccacg gtaagacaac
    51121 ccttactgcg gcaatcacaa agagtcttgc attcaaaggt cttgcagatt tttccccgtt
    51181 tgactcaatt gacaaggcgc cggaagaaaa agcaagagga attacaatca acgtatctca
    51241 catcgagtac cagacggaga agagacatta cgcgcacatc gactgccctg gacacgctga
    51301 ctatatcaag aacatgataa ctggagctgc acagatggat ggagcaattc tcgttgtcgc
    51361 cgcaaccgac ggtgtcatgc ctcagactag ggaacacgtc ttgcttgcta gacaggtcaa
    51421 tgttcccgca atggttgtgt atataaacaa ggtggatgca gttgatgacg aagagctggt
    51481 agagcttgtc gaggaagaag taagagagct tctctcctcc tacgagttcc cgggtgatga
    51541 actccctgta attaagggtt cagccttaaa ggcccttgaa gcggaaagtc caaatgaatg
    51601 gacagagagc atttacgaac tgttgaaagc atgcgacgac tacttccctg aaccagtacg
    51661 ggagactgac aaacctttcc tgatgcccat agaggacatc ttcacaataa cgggtagagg
    51721 tactgttgtt acaggaagaa tcgagcgtgg agcggtccat gttggggatg aagtagagat
    51781 tatcggactg tcttacgaaa cgaagaaaac agtgtgtact ggagtagaaa tgttcaggaa
    51841 actccttgat gaaggacagg ccggcgacaa cattggagct cttctaagag gcgttgcaaa
    51901 ggaagaagtc aagagaggcc aagttcttgc caaaccgggt tctataacac ctcacaagaa
    51961 attcacggca aacgtctacg ttcttaaaaa ggaagaagga ggacgccact cacccttcac
    52021 aaaggggtac aggccccagt tcttcattaa gaccgccgat gtaactggag aaattgcaga
    52081 tcttcctgag ggcgtcgaaa tggttattcc tggtgacaat gtagaaatga caattcagct
    52141 catttacccc gtcgcaattg aaaagggtat gaggtttgca atccgtgaag gtggaagaac
    52201 agtgggcgcc ggagtcgtca gttccataat tgaatgataa taggtctttc caggagaagg
    52261 gagaaaatct cccttccctg ttaacagaag gagggaacat ttaggcatgg ccaagcaaaa
    52321 aatcaggatt cgcttgaaag cttacgatca caaactactt gacttgtcgg ccaagaagat
    52381 cgttgaagct gtaaagctca ccaatgccaa agtatcaggt ccggtgccat tgccaacgga
    52441 gaggacgctc tacactgttc tgacttctcc ccataagttt aaggatgcaa gagagcagtt
    52501 tgagaaactc gttcataaga gacttatcga aatactagac ccaaccccta agacaataga
    52561 ttctcttatg aaagttgacc tccccgcagg tgtagatgtg gagattaaac tgtaaagaga
    52621 cttcggaggt gtgatagatg aaaggtatat tgggtagaaa actcggtatg acaactatat
    52681 acaaagacgg gaaagccttc ggagttaccg tagtgaaagc cggtccatgt actgttgtac
    52741 aaaaaaagac aagtgatggt ggcgaatatg acgccattca agttggattc gaagagctta
    52801 cgcctgaaag agcgaaaaaa gtactcacga agcctttggt aaagaagttc gaggctgcaa
    52861 aggttaagcc tcatagaatt ctcaaagagt taaaagttgg taacatcaat gatttcaatg
    52921 ttggtgatat catagaagct cgtgtctttt ctgaaggcga gaaagtagat gtaactggtt
    52981 tttcgaaggg cagaggtttt tcgggcgcta tgaagaggtg gaacttcagg ggcggagaag
    53041 cttcacacgg ttcgaaattc cacagagaac taggttcagt aggtaatcac actgagccgg
    53101 cgaagatttg gaagggtaag aaaatgcccg gtcaatacgg taacgagaag aagacagtga
    53161 agaatctaac cgtagtaaaa gtcgatgcag aaaacggact cttagccatt tatggcgcag
    53221 ttccaggagc gaggggcggc ctcttagtaa ttaagagcgc aaacagatag ccccgaaaat
    53281 gtttggaagg aggacgcaac aatggctcaa gccgacgttg tgaatattgc gggccagaaa
    53341 gttggcactg ttgaactgag cgagagcgta ttcaatgtag aaccgaacga agatttgatg
    53401 ttcagatacg ttaatatgca actttcaggt agacgtgcag gattggcttc ggcaaagaca
    53461 agatcagaag ttaggggtgg aggaagaaag ccctgggcac agaaacacac aggaagggca
    53521 agagttggtt caacgagaag tccactttgg aggcatggtg gagttatcca cggaccgaag
    53581 ccaaaagact ggtctatgaa gttgaccaag aaaatgaaga aggtagctct aagatctgcc
    53641 ctaagtctaa ggcttaaaga aggaaatcta attgtcctcg atgatctcaa gtttgacaga
    53701 ccaaagacga aacagcttag ggaggtcttg aataaccttg gactcgataa gacgcagaag
    53761 actctcttcg tccttccatg gcaaaaggac gaataccaaa atgtacgact ttcaggaaag
    53821 aatatttatg gggttaaggt aattatcgcc gataatcctg gaaacaaagc cgtcaatagt
    53881 aaagaaaata ttgacgggct caatgtgtac gacattgtga atcatgaaaa gcttgtgatc
    53941 acggcagacc ttgttcgcaa gatcgaggag gtgcttggac gatgatcgag aaaaagtatt
    54001 atggcgatgt actccttaga ccgctgatca ctgaaaagac agttgcttct ggtgaactaa
    54061 acaagtacat ttttgaagta catagagatg ccaataagca gacagtcaaa gacgccgttg
    54121 aaaagctttt caatgtaaag gtagaacagg tgaacatcat gaacatgaag ggcaaaccta
    54181 agaaacgtgg cgtctttgtt ggcaagtcga gatcctggaa aaaggcaatc gttacgcttg
    54241 taactgggta caggattaaa gaactcgaag gccaacacta aggtgaggtg agatgatatg
    54301 gcattgagaa agtttaatcc cgtcaccaag agcaggcggt tcatgcttct tccggacttc
    54361 aaagaagtca cgaagagcga acctgaaaaa tctcttttgg agccaataaa gaggaatgca
    54421 ggtagaaacc atcatggtcg aataactgta aggcaccaag gtggcggaac taagagacga
    54481 tatagaatta tcgatttcaa gagggaaaag atgggaatcc ccgcaaaagt catatccata
    54541 gaatatgatc cgaacagaac ggcaagaatt gctctcctgc agtatgtaga tggtgagaaa
    54601 cgatacatgc tggctccaaa agggcttaaa gttggagaca gtgttcagaa tggagatatg
    54661 gcggaaatct cggttggaaa ttcgatgtca ctcgagcgaa ttcccgttgg cacgattgtt
    54721 cacaacatcg agttcttgcc cggcaaaggt ggtcaaatag ccagggcagc gggaacctat
    54781 gcccagctga tggcaaaaga aggaaggtac gcgctcctga ggatgccttc aggtgaactt
    54841 agaagggtcg ttgtgaagtg catggccact attggcatgg ttgggaatga ggaacactcg
    54901 aatgagatac atggaaaagc aggtagaaag agatggcttg gcgtaagacc ttcagtgaga
    54961 ggaatgacta tgaacccagt tgatcacccg atgggtggag gagaaggacg atcaaagggc
    55021 catcttccgc agagtccttg gggacagcca gccagaggtt ataaaacgag gaagaataag
    55081 aagccttcag atagcctcat agtgaagcga aggaaccaga attgatcagg gggtgtagtt
    55141 gaagatgtca agatctaaga agaaaggccc gtacgtacac ccgagtctct tgaaaaagat
    55201 aagagaactg aacgaaaagg gagaaaagaa gcccataaag acttggagca gagcatccat
    55261 gattcttcct gagatgatcg gacatacaat cgcagtatac aacggaatga agcacatacc
    55321 ggtttacatt tcggagaata tgatcggtca cagattgggt gagttctctc ccaccaggag
    55381 gttcggtggg catgccgata agaaaagcaa gaaaggcgaa gttagatgag gagggacgct
    55441 gatatgacgc agtcagtgca gagaggcaag cgttccgttt tccacaggaa gcgcaaagaa
    55501 gagaaagcag caacgcctgt aactcaggcg aaggcggttg cgaagttcgt tagaatttcc
    55561 cctagaaagg ctagatcagt ggtaaatgcg ataagaaaca aagaggttgg ggaggcgttt
    55621 cagatactag aattttcgcc aaagaaggcc tcgcgtctcg tatacaaagt gttgcgttct
    55681 gcggtagcga atgcagaaaa caactttgga ttgaatgtgg acagtctgta tgttgagaaa
    55741 gccgtagtag atgacggccc aagaatgaag cgactgtggc ccagaggaag aggcagagca
    55801 gatatacagc agaagcgctt tagccacata accgttgtag tagcaaaccg cgaagagatt
    55861 agcaatcctc aggagtgagg tgacataagt gggtcagaag gtacatcctt atggattcag
    55921 gcttggtgtc agtaaagatt ggaaagctcg ctggattaat gagaagaatt acaaagaata
    55981 tcttcttgaa gatcttaaaa tcagggactt tttgaagaag aactacatgg ctgccggagt
    56041 gtcggacatc tatattgaaa gacctgagcc cggcaaagta gttattacaa tcaagtgcgc
    56101 cagacctggt gtgatgattg gaaagaaggg cagtgaagtg aagctgctca ggcagagact
    56161 ggaaaagctt ataacaagac agttccagtt aaacattgaa gaagttaaaa cacccgagac
    56221 cgatgctatc cttgttgctg aggatattgc atctagaatt gagaagcgag cttcctacaa
    56281 gagggcaatg aaaagagcaa tattcaccgc catgagaaag ggagctagag gtatcaagat
    56341 aatggtttcc ggaagactga acggtgccga tattgccagg acagagtggt acctggaagg
    56401 aagacttcct ctccagaccc tcagagctga tctggactac ggttacacca ctgccttcac
    56461 gaagatggga attatcggag tgaaggtttg gatttacaaa ggcgacgtac aggtgtaatt
    56521 gcaagagggg aggtagttca taatgttaat gcccaaaagg gtgaaatata ggaagcaaca
    56581 aagaggtaag atgaatggaa aagcgaaagg cgggactatt gttcattttg gcgaatgggg
    56641 aataaaggcc ctcgaaccac attggattac tgctcagcag atagagtcct gcagaatagc
    56701 gatcacaaga acattgaaga ggaatggtaa tctctggatc cgaatcttcc ccgacaagcc
    56761 cattacgtca aaaggaattg gagtcagaat gggaaaggga aagggagacg ttgaaggctg
    56821 ggtagcagtt gttaagcccg ggaagatcat gttcgaaata ggcggagtgc ctgatcaact
    56881 tgcaaaagag gcccttgaaa aagctgcttc aaaactacct atcagaacaa agatagtacc
    56941 gaggtatcaa ataggaggtg agctctgatg aaggccgttg aacttcagaa gttcacagac
    57001 gaggaactca ctcagatgct tgaagattcg aagagaaagc tgatggacct caggttccag
    57061 ctagaaatga acaggttgaa gaaccattcc cagataactt cagtaaaacg cgacatagct
    57121 aggataaaga ctatccttcg cggtcgtgag ctggggataa ggaggtagtc cgccatgcca
    57181 agaaaaacac tggtaggaac tgtagtaagt gacaagatgg ataagaccat agttgtgagc
    57241 gttacaagaa cacatataca tcccttctac gggaagacta tgaaaactac aaagaagtat
    57301 cacgcagatg accctgaagg tcttgcggga atgggtgata cggttgagat tgaggaatgt
    57361 aaacctttaa gcaagatgaa gaaattcaag cttgttaagg tcttgaagaa ggatgtctac
    57421 actggagaaa ttcccgaaac tcccgaggcc gttgaagatt ccggaggtga cgagaaatga
    57481 tccagctgga atcttatttg agggttgccg ataattcagg tgctaggatg atcaaagtca
    57541 tccaggtaac tggcggatac agaaagagat cgggaaccgt aggtgatata gttgtcgcag
    57601 cagtaagaga tgtggcacct aacacggatt tcaagaaagg agatatcgta agagccgttc
    57661 tagtgagaac cgccaaggaa attcgaaggc cagacggcac ttacataagg ttcgatgaca
    57721 acgcagcggt tatcatcgac aagcagaacc agcccagagg aacacgtgtc tttggacctg
    57781 ttgctagaga gattagggac aagggttttt ctaagattgc ttctctagcc caggaagtct
    57841 ggtgaggagt gagctattat gagtagagtg aagaaggaag ataccgtaat gataatatct
    57901 ggaaaggata ggggcaaaaa aggcaaagtc ctcaagacat tcccctcgga aaaaaagata
    57961 atcgttgagg gagtgaactt cacaaagaaa caccagagac ctactaacca gtatcgtgaa
    58021 ggtggaataa tcgaaagaga gtctcctatt tacgtttcga aagtaatggt tgtctgcccc
    58081 aactgcaata agcctacgag agtcgcacac aaaattctcg aaaacggaga aaaagtaagg
    58141 tcctgcaaga agtgcggcga gataatcgat aaggtttgat ggggaggagt tgttgatggc
    58201 atacgaatac gtccctttga aagatagata tgaaaatgaa gtaattccgg ttatgactaa
    58261 agagtttggt tataagaaca ggctcgaagt tcccagagtc tctaagatag tagtcaatat
    58321 gggaataggt gaaggttcaa gaaacgccga ccttctcgat ctgcacggca aagagctcat
    58381 gacaattgta ggacagaaac cggtagttac taaggctaag aggagtatcg cgaactttaa
    58441 actccgtgat ggaatgccgg ttggtttgaa agtcactctc cgtggtccga ggatgtataa
    58501 cttcctctac aagctgataa atatcgtgct tcccaagctg agggacttca gaggtgttga
    58561 tccagactca tttgacggga gagggaatta tgcaatgggg cttcccgaac aattggtctt
    58621 tcccgaaata gtaccggatc aaatcaagcg agttcaggga atggacataa ttgttgttac
    58681 tacggccagg accgatgagg aggccagaag attactagcc cttctcggac ttcccttcaa
    58741 gagagtcatg gtctaaggag gtaagtgcat ggcgaagaaa tctatagttg aaaagtggaa
    58801 acgcgaaccc aagttcaagg ttaggaaata taatagatgc aatctgtgcg gcagaccgag
    58861 agctgtatat agagagttcg gtctttgcag agtgtgcttt cgacagctgg cttccgaggg
    58921 aaaactcccc ggtgtaaaga aagcaagctg gtgaggaggg aattgtaatg tggagtgatc
    58981 ccatagccga tatgctcact agaatacgaa atgccaacgc tgcctttaag gaaagcgtgg
    59041 atattccagc ttcaaacctg aaaaagaaac ttcttgatat tctgaaggct gaaggctata
    59101 tagatgatta taagtatatt gaggatggca aacagggtgt tctcaaggtc tttctgaaat
    59161 acaagggaga ccggagacac aaggttaacg tcatatctgg aatcgtaaga atttctaagc
    59221 ctggtaagcg cttatatgtt aacaaggata agcttccaaa ggtcaaatca ggtatgggaa
    59281 ttgcaatagt aagtacatcc agcggagtga tgacagacaa gcaggcccgt tctcaaggcg
    59341 tcggcggcga agtcatctgc tacgtatggt agggaggaat tcaaatgtct agattactta
    59401 agcattcgat aaaaatccct gctggtgtgt cctgcaaaat tgaaggaagt gttatctctg
    59461 tcaagggacc gaaaggtgag ctcaaacaag aactccttcc tctggttaag atcgaggccg
    59521 atgaaaaaga gatatgggtc aagtcaaacg aagaagttgt tattagaaag agtgattaca
    59581 agagattggc taagtatgct ggtactttct ggtcgctcat aaataacatg attgttggcg
    59641 ttactcaagg ctttgtcaag gagctggaaa tagtgggagt gggatacaga gcacaacttc
    59701 aaggaaagaa gcttgtaatg aaccttgggt atgcacatcc agttgagatt gaacctccgg
    59761 caggagttga gtttgaagtg cccgcacctc aagcaatagt tgtcaaggga attgacaagt
    59821 atcaggttgg tcaggtagcg gcaaacataa agcgctggag aatccctatt gtttattcag
    59881 gaaaaggtat tcgttacaag ggagagcaag tcagaacgaa agtcggtaag aaggtctaat
    59941 caaggaggcg gctggagtgt tcaaacataa agacaagaag gaacaaaggc gaaagaggca
    60001 tcttcgcgtc aggtctacag tttccggaac accggagaga ccaagactgg cagtcttcag
    60061 aagtgagaag catatttatg cccagcttat tgatgattca aagggaatta cccttgtttc
    60121 tgcatcgact gtagataaag acgtcaaggc ttcaatagag aagtcatgga atgttgatgc
    60181 cgctaagaaa gttggggagc tcctcgcaaa gaaggcgaaa gataagggaa taagcagtgt
    60241 agtcttcgac cgtgggggtt ttaagttcca tggaagaata aagtcacttg ccgagggcgc
    60301 tcgagcaggc ggactacagt tctgaggagg tgaagaagat ggctgaagag atgaagaacg
    60361 accgcaattc gaaatcttca cgtgaaggaa gaagaaaccc cagagcttcc agaaacgctc
    60421 ctgtggaaaa acaggttgaa gacaacgaat ttgaagaaag aataattgaa ataagaagag
    60481 ttacaaaagt agttgcagga ggaaagaatc tttcttttag agtagtcgcc gtcgttggta
    60541 accgtgatgg gaaggttggt cttggtatag ggagcgcaag ggaagttccg actgcaataa
    60601 gaaaggccgt tcttgaagcc aagaagaatg tcacggaggt cgcggtgaga aatgagactg
    60661 ttcctcacga gacagtcgga cgacaggatt cagcaagaat aatgttaaag ccggctggtc
    60721 ccggaactgg gattattgcg aattcgcctg taagagccgt tgtcgaactt gcaggtgtga
    60781 agaatattct gacgaagtcg cttggatcat ctaacacact taatatggct agagcggcct
    60841 tgaacggact actaagtctg agatcccctc aggacttcgc aaaactcagg gacatacccc
    60901 taaagacagt cttccacggt ttcagcgagg aggcaggtca gtaatggcaa agagtttaag
    60961 aatacgatta atcaaaagtc cgattggcta taacagaaga cagctgaaga cggttaaagc
    61021 attaggcctt ggaaagctgg acagtgagat tgttcagccc gattctccac agatcagggg
    61081 catggtgaat tccataagac atcttcttgc tgtcgaagaa cttgatgact gaagggggta
    61141 ggaagatggc ttttaaagtt gaagatctaa gtccgacgcc cggttctaga acaagcgaaa
    61201 aaagaatagg acgcggtata ggttcgggaa tgggtaaaac cgccaccaga ggccataagg
    61261 gtcaaggaag agcgacaggt aaggtggctg cacgcttcga gggcggacag acacccctat
    61321 tcagaagaac accgataaaa gggttcaaga accgaggagc agttgtttat gctaccgtga
    61381 acatttcgac tctcgaggag agattcgaaa gtggcagtga gatttcaccc gagatactcg
    61441 ttcagcgaaa gatactcaaa gatcttaaag acggagtgaa gatacttgcg agaggtgaac
    61501 tgacaaagtc ttttattgtc aaggcaaatg cttttagcgc tactgccaag gagaagattg
    61561 aatctgctgg tggaaaggca gaggtgatct gacaatgtgg aatgccctga agaacgcgtt
    61621 caagattcct gagctgagag atagaatcct cttcacgttc ctcgcattgg cagtcttcag
    61681 acttggtgtg tacataccga ttcccggaat taatatccaa gcctggtctg cgtacttcgg
    61741 cactctatct acaggcggtg ccggaggctt catcggcttc tttgatgttt tcaccggcgg
    61801 agccgttgag cagttttcga tattcttaat gagcgttact ccctacataa acgctcagat
    61861 tatgcttcaa ctgctgactg ccgttgttcc tagtttgaag gagatgctta aggaaggcga
    61921 agagggaaag aagaagtatg ctcgatacac aagaatgctg acgattggac tcgcgggtct
    61981 tcaaggattc ctgatatcat tcggtctctc ttcgaacacg gctatcttgg cgattcccag
    62041 caggttgctc tttgtgttgc tggcaacaac cacccttatc ggtggtacaa tgttccttct
    62101 ttggctggga gaaagaatca cggaaagggg aattggaaat ggtatttcag ttctgatttt
    62161 cggaggcatc gttgcaagat atcctgcttc cattatgcag gtagtggtag ctttatcgcc
    62221 gttacagtgg gggatattac ttgcgatcgc tctgttcacg gttgtggcgg ttatctatgt
    62281 tcagatggga gaaagaagga tcgaagttca gtacgcaagg agagttactg gtagaagggt
    62341 gtatggtgga gtttccacgc acattcctat caaggttaat caaggtggag tcataccgat
    62401 aatattcagt tcggcaatta tgatgttgcc acagtttatt gctacggctt tccccgaggg
    62461 aagtggcggt aggaatatta tgcagagtct ctttgctcag acatcacccc tctacatatt
    62521 gctttatgga ggaatggttt tcttcttcac gttcttttat agttcgcttg tttttgatgt
    62581 cagagaagta tcggataaca tacgtaacta cggaggctac atacccggaa taaggccggg
    62641 cttttcaact cagcagtata ttcagagagt ccttaacagg gttgttttca tgggagccgt
    62701 attcctggtt gtgattgcat tgcttccatt agttataggt gggattttca gtattggagg
    62761 cctcgcaatt ggaggaacgt cgactctaat tgctgttggc gtagctattg atatattgca
    62821 gcagatggaa acacacctta tggtaaggca ttatgaaggg ttcgtaaaga aaggcaagtt
    62881 acgaggaagg agataagatg aacgttattc taatgggtcc tcccggagca gggaagggga
    62941 cacaggcaaa acggatagcc cagatattta agattcctca catatcgact ggagatatgt
    63001 taagggaagc cgtggctgct ggcaatgacc ttgggctgaa ggtcaaagag attatggata
    63061 aagggcttct cgtccccgat gatttgatga tcgaccttgt gagagaaagg ttgtcgagag
    63121 aggataccag gaatgggttc attcttgacg gtttcccaag aacagttgag caggcggctg
    63181 cgcttgatga aatgctagag gatctaggga gaaaaatcga tgtagccttg ctcgttaatg
    63241 cagatgaaga agaagtcgta aaacggattt ctagcagaag agtttgccca gagtgtggca
    63301 aggtctacaa tctgctgacg ataagaccga aagttgaagg ccgctgcgat aacgatggcg
    63361 ccgagttgat ccagagagat gacgatatgc ctgagacagt tcgagcaagg tacagagtct
    63421 atttggagaa aacgaagcca gtgatccagt attactcgag taacaagagc cagtttttgg
    63481 aagtggacgg gaccggagag attgatgttg tgactgacaa aattgtggaa catctggagt
    63541 caactagaaa tggttaggtt gaaatctcca gaagaaatat gtaaaatcga aattgctgcg
    63601 aagattgtcg cagaggtttt ggctgagatg gagtcttatg cagttgaagg agcctctgct
    63661 ctcgatatgg aaaaagcggc agaagagttg attcataaac gaggcggcat ccccgcattc
    63721 aagggatata gtggttatcc ttacactcta tgcgtttcag taaacgagga ggtaattcac
    63781 ggatttccct tgaaggaaaa aatctttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    63841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    63901 nnnnnnnngt ttcagtaaac gaggaggtaa ttcacggatt tcccttgaag gaaaaaatct
    63961 ttgtcgatgg cgacatagtc tccgtggatt gtggggtgaa caaagacgga ttcattggag
    64021 atgcggcaaa gtctttcgta gtaggtagtt acaggagtga aaaggacaaa ctgcttctac
    64081 agcgaacgga agaggcgctt ctaaagggta tagaaatggc tgtcgcagga aacagagtcg
    64141 gcgacatcgg ctatgccgtt cagaattgtg tagaaagcgc tgggctgtct gtaataagag
    64201 attacgttgg gcacggagtc ggcctcagcc ttcacgagga tccacaggtg ccgaattacg
    64261 gaagacaggg aagcgggatg cttttaagaa ctggcatgac ccttgcgatt gaaccgatgg
    64321 tcgcaagcgg acactatagc ttagagattc tggaagacgg atggactgca gtaactgcgg
    64381 atagatcgcg tgctgctcat tttgagcatg acattgcgat tcttgaagat ggtccaaaga
    64441 ttctttctag tttgtgagga ggatcactgg tgtcggataa gagcgatatc attaagatgg
    64501 atgggacagt tgtggaatcg atgcctaatg cgaccttcaa ggtcgaactt gagaacaatc
    64561 acactgttct agctcacatc tctggaaaaa tgcgcaagaa cttcatcagg ttgatcccgg
    64621 gtgataaggt cgttgtggaa gtgtcaatat acgacctgac caaaggaaga attgtttatc
    64681 gtaaaaggat agacaaagga ggagaaacga attgaaagtc cgcgcttcgg tcaagaagag
    64741 atgtgaacac tgcaaagtga taagacgcaa tggcagaata agagtcatct gcgaaaaaaa
    64801 tccaaagcac aaccagcgtc agggttaagg agggatatag atggcacgta tccttggtgt
    64861 tgaacttcct aataacaaga agactttcgt tgccctgacc tatatctatg gtataggtta
    64921 tacaagatcg aacgagattc tttcaggaac ggaaatcgat ggcaataaga gagcaaaaga
    64981 cctgactgac gaagaagtta gtaaaattgc gaagtacata aatgagcatt acaaggttga
    65041 aggcgagctc agaactgaag tcgacaggtc gattaagaga ctaatcgaga taggaagtta
    65101 tagaggttac aggcatagaa acgggcttcc ggtacgcggt cagaagacgc attcaaatgg
    65161 aaggaccaga aagggttcta gagccagtaa gattcgtaag aagagctaaa cgggaggtaa
    65221 aagtgaatgg caaagagacc agcagccaag aaaaaaagga aactgacaac tgatagggga
    65281 gtagtgcata taaagtcctc tttcaacaac acaataatta cgttgaccga cccagaaggg
    65341 aacactctga tgtggacgtc aggtggaacc gccggatact ctggatcgaa aaaatcaaca
    65401 ccctatgcgg ctcaactggg cgcagataag attgctaaag aggccatcaa gctagggatc
    65461 acgagagttg gaatagaagt caagggccca ggttctggaa gggaagctgc aatcaggaca
    65521 attcaagcgg caggtcttac tgtggagacg ctcaaagata tcacgccact gcctcacaac
    65581 ggttgcagac cacgcagaag aagaagagtc tgatttagga gggagttgaa tggccagata
    65641 tacaggacca gtgtgtaaac tttgtcgtcg tgaaggattt aagctgtatt tgaaaggtga
    65701 aagatgtttt tcaccaaggt gttctcaggt gaagaggcct gtcgcccctg gacaacacgg
    65761 agcctccact agaaagctca cccaatatgg catgcagttg agatctaaac aggttgtaaa
    65821 gaggatttac ggtgttcttg aaaggcagtt cagacgatat tttgaagcgg ctttgaaaaa
    65881 gaacgaagaa acgggaactg ctctgatgaa gattcttgaa tcaagacttg acaatatcgt
    65941 tttcagaatg ggattcgcct cgagcaggag gcaggcgaga cagctggtta accacggaca
    66001 cgttcttgtt aatggtagaa gggtgaacaa gccttccttc aatttgagag tcggtgacat
    66061 cgtggagatc aaagagaaga gcagaagtgt tttaccgatc aaagaagcgg cagaagctgc
    66121 aaaagagaga acagcctatc catgggttga ggtagactac gagacattta gaggtaccta
    66181 cctgagatat cctgtaactg aagaagtaga gattccagtt gatctgcagt cgattattga
    66241 gctctactcc aagtaaatcc gattcttacc ttcttggaag gaggtgtgtg cccgaattct
    66301 gggcgaaaca atgttgacat caataatgcc aaaaaacttg cgaattgaag aagaaagaga
    66361 ggatgataac agctatttta caagattcat cctttctcca atggaaagag gatatgccac
    66421 tactatcggg aactcgctta ggagggttct cctctcatcg ataccgagta tggcaatcac
    66481 aagactaaag atacctggta aatatcacga atacgatgtt attgacggag taaaggaaga
    66541 tattctcgaa ataatcctta atttcaagaa agtccagtta aaacccgcac ttgaattctc
    66601 tgatgcggtg aaactcacac ttgagacggc aggaccagga gtcattacag ccggcgatat
    66661 taaaactcct actggggtag aggtagtcaa cccgtctcaa tacatagcta ctctgaacac
    66721 agattcagtt atttatatcg agcttttcgc tgaaattggg cgagggtttg ttcccgtgtc
    66781 agaaatggag atagagcacg acgttgagat gatttacatc gatgggatct tcagccctgt
    66841 gttaaaagtc aacttccaga ccgagaacgt ccgggttgga aagagaacag actacgacaa
    66901 gcttatcttg gatgtgtgga ctaaaaaatc gataacccct tctgatgcgc ttttgagagc
    66961 aactgacata cttattaggc acttcgaggt cttctccaac ggtctaggca agccgggtca
    67021 aggtcttaca agctcagcgc tcaaacagga agaagaggaa atcatttctg aagaagccga
    67081 agtcactgtc gaaacccccc aggtggaaga acaggactcg aattcccttg gagcgaagaa
    67141 ggttgaggaa ctcgatcttt cggtcagatc tcttaactgt ctcaagcgcg acaaaatcaa
    67201 cacaattggt gatcttctcg acaagagcga agctgatctt ctgaggatca ggaacttcgg
    67261 agagaagtcc atgctggaag ttgtcaagaa actgagggaa aagttcaaca tcgtactcaa
    67321 gaaagaatga ttttgaggag gcctattagc tatgcgccat agagtcagtg gaagcaaact
    67381 aaatatgccc cacagccagc gagtcgcttt aatgcgaaac ctggccaggg agcttttcga
    67441 acatggaaca atcgttacta cagtcactag agcaaaggag cttagaccct ttgttgaaag
    67501 cattattact aaggcaaaga aggcttctct actctcagtc gagatttctt ctaagggtcc
    67561 tgacagtaca gaaacacagg cactgaagtc aaggaacctt gctttgcatc gagagataaa
    67621 caggaacttc aacgacagaa agcttgtcaa aaagatctgt gatgaagttg cagttaagta
    67681 ccacgaaaga aacgggggat acaccagaat agtaaaatta ggtatgagaa ggggcgacgc
    67741 aagtgaaatg gccgttcttc agttgataga caacgaagaa aaacaggaaa agaagcccga
    67801 gaagaaagct gaaaagaaac aggacaaaaa gtgatggcta ccgcaaggta gccattcttc
    67861 ggaggtgtgg caatgaagat atttcttgac acggcaaaca ttgaagacat tcgtgaggga
    67921 atgaagcttg gcctcgtcga tggcgtgact accaatccaa cattggtttc cagagaagga
    67981 gttaagttcg aagaccgagt tgtggagatt tgtgaaacgg ttaagggccc tgtatcggcg
    68041 gaagtaacgt ccactgatta cgaaaacatg gtattacagg cgagagaact cgcgtcactt
    68101 aatgagcatg tggttgtgaa aatcccaatg acaagagatg gaatgcgagc agtaaagaca
    68161 ctttgtggcg agggaatcaa gacaaatgtt actttaatct tcaattctct tcaagcaaca
    68221 cttgctgcca aagcaggcgc aacatatgta agcccatttg tcggaaggct cgatgatatt
    68281 gcaagcaacg gaatggaaat agttgaggag attgtccaga tattcgcgaa ctacggttac
    68341 agcactgaga taattgtggc aagcgtgaga catcctatgc atgttcttga agcagctctc
    68401 atgggagctg acatcgtgac gattccctat gaagtacttc tcaaactatt caatcacccg
    68461 ctcactgata taggaattga aagattcatg gatgactgga aacgctatca gaaacagcaa
    68521 ggcaaataag ctttgatttt ttccgcgtaa gtggtatatt atttctgaaa gaacttgtag
    68581 ctcaccggag acaggtctcc catgctttct tacaaatatt gctagcttca tattctttat
    68641 aattcctaaa gatacggaag gagggtaggt acattcttcc agtggtgatt gaatgtaccg
    68701 tcaaatttga gcccaagaaa gaagaaaacg gaagaaacac cgaacgaact tgatgaaagt
    68761 gtcgtgagtg agaagaagcc gaccagaaag agaactacgc gaaagaagac tgaagtcaag
    68821 aagcccgaaa aaaaggagga aacacttagc gctacttcta atgagatcga aagaagcatg
    68881 aaagaaaatg aggtttcatt aaaaggagat gccgaaaaga agatggattc ggaatctgag
    68941 tttcaggaat caactagtga gagcgctaca gatgaatcga aggagcttcc aagaaaggac
    69001 aaagacaagg acaaagacaa ggacaaagac aacataaggg aagtcgaaat cgatatctca
    69061 cagctccagg caatgcctat cagagaagtc tacaagcttg ctaggaagta tgatgtccaa
    69121 ggctatacgc agatggcaaa gaaagacttg atattcgctg ttcttaaagc ccaaacggaa
    69181 tcttatggtt acttcttcga tcagggagtt cttgaaatca ctgacggggg atatggcttt
    69241 ctaagaactc tggataacaa ccttcttccg agctctaacg acatatatgt gagccagtct
    69301 cagataagaa gattcaacct tacaagtggc gatacagtgg ccgggcaggt aagacccccg
    69361 aaggaaggcg agaaatactt tgcgctcttg agaatagagg ctattaatca caaggcgata
    69421 aacttcgctg cagaaagaat tacttttcag aatcttacgc caatccaccc tgaagacagg
    69481 ttgattaccg agacagattc tcatataatg agcacaagaa tggttgatct cttctcccca
    69541 attggaaagg gacagcgagg attgatagtt tcacctccca aagcggggaa gaccatactg
    69601 ctgaaggagc ttgctaacgg aattgccgag aatcacccag acaccataag aataattctg
    69661 ttgatcgacg agagacctga agaggtcact gatctcagaa gaagcacaaa tgctcatgta
    69721 atagccgccc catttgatat gcatccagag aagcaaatta gagtcgcgga gatgactatc
    69781 gaaatggcaa aaagattggt cgaattcaat tacgatgttg tcattctgct cgacagtatt
    69841 actagactag caagagccta caatctatat gttccgccca gcggaaagct tctctccggt
    69901 ggtgtggatc cttctgctct ttacaagccc aaatacttct ttggagcggc tagaaacata
    69961 gaagagggtg gaagccttac gataatcgct acagcgctcg ttgaaacggg gagcaagatg
    70021 gatgaagtta ttttcgaaga gttcaagggt actggaaata tggaactgat tctatctaga
    70081 cagctggcca acaagaggat gttcccatcg atcaatctta ctctttccgg tactagaaga
    70141 gaagaactac ttcttccttc agatattctg agaaagatct ggattctaag acgaatgctg
    70201 tcttcaatgt cggaagaaga gggattgaga ctaatcatgg agaaacttcg tggcaccgga
    70261 tcaaatgacg aattcttgga tttgattgaa atggaaaaga agcagtacta gatgggagat
    70321 aatgatggtc agacgcgtaa taggaattga tctgggtgga acagagacaa agatagggat
    70381 agttgaggaa gacggcaaga tagtcgaaaa aaaggtgatc cccacaagag tgtcagaagg
    70441 tagaactact gtcgtcacaa gaattggtga agcgataaat gagctccttg ttcagtcggg
    70501 tatcgactca aagcaaatta tcggaatcgg agttggttca cctggatcga tcgaccacga
    70561 cacgggaact gtgctgtttt ctccaaatct tcccgattgg tcggggttcg ggctggcagc
    70621 tatgctcgag agagtcactg gaataagaac atttgtagag aacgatgcaa attcctttat
    70681 tctaggagag tgggcattcg gcgagttcaa aggcagccaa cacatggtag gcctcacatt
    70741 aggtaccgga gtcggagggg gagtgataac ccacggtatt ttgatgacag gtagcaaagg
    70801 ctatggaggg gaacttggac acactatagt tgaaccggaa ggaccagttt gcggttgtgg
    70861 ctctcacgga tgtcttgaag ctcttgcttc ggcgactgcc ataataaatc tggccaggga
    70921 atattcgaag agatttccgc agtcaacgat atttgcgtcg cctgaaataa acgctaaagt
    70981 ggtctttgac gcggcacggg agggtgatct cgcagcaact ctgatcgtag aaagagcaac
    71041 cagagccctg gctattgcaa tcggaaattt catacatgtg ttcaatcccg aacatattgt
    71101 cattggcggt gggatcagca gagctggaga tttgctaata aacgggataa gggaaaaact
    71161 cccggctttc gtaatgactt cttttaacgg gaccttcagc atcactctga gcaaattggt
    71221 agagaatgca gggataacag gtgccgcgtc gatagtcttt tacaggacta gttgattctt
    71281 caatcgttga gtcaggatat gtgcttgtct agtaggttcc ggaattctgt actttccaat
    71341 tgttcttatt gataacctga gagcactttc cgcagttatt ctatttcccg gtgaaatgaa
    71401 aaccggtttt acactcgttc ttgtacatag cgcatatcct agcaatctac cgcctggtga
    71461 gaggattctg ctgctttgtc ctttctctct cgggaggctt tcgacctttc catagagaag
    71521 gcttttcgca acgcctatcg tcggtatttc tacgaaaagt ccaatgtggg aagctatacc
    71581 aatccctctg ggatgagcta tcccgtgtcc atcaaagaag agcaggtcaa cttccggtga
    71641 tctagacagc gcatcaagta tagcgggccc ctccctaaat gacaatagac cggggatgta
    71701 aggcgtctca acctttttaa gagaaaagaa atggtcaact atggatagag agttaaactc
    71761 cataatcaca attacggcga gcgcaatctc tctaaaagga aaagaaacgt ctattcctgc
    71821 cactagtctt ggctcccgag agtagtcctc aaatgtaagc ttgtccctga gtttcttttg
    71881 taagtctata gcttgaaccg gttccatcga ccatgaatga atatattcga tattcatgtt
    71941 gttctctccc aaaggaagaa atctatcgct gttgcttctg acaatgatta tgccacatag
    72001 atgttataat ttatgaaaag ctcttcaaat ggaggtgcat gatgaaattc gtcgcagctc
    72061 gttcagaaat cctcacaaga ttagaatcgg tttctggggc agttgctccg aaaagcgtga
    72121 agccaattct ttcgggcata tatttttcaa tgaaggatga ctcaacggtt aagcttcagg
    72181 ccaccgatct agaaacggca attactacag agctgagacc aaagcacata gatggaaggt
    72241 gcgattttgt catagatgcg cgtctggtac tcgaaatagt cagaaatctt ccagaaggag
    72301 aagttgtttt tgaaacggaa gacacaaacg ttatcattag ggagggaagt gcacgcttca
    72361 ctcttccaac aatggatccc gacgagttcc ccattgttga accaacctcg ggaggacttg
    72421 aattcacagt gtcggtttca tcgattgaac tgatgataga tagagtgatt ttctgtgctg
    72481 ctagggatga attcatgaga aatctgaaca gtgtctactg ggaattcgag gatggttacc
    72541 ttcgacttgt agctgcggat ggattcagga tggcgctctc cgaagaaaga attgacctta
    72601 gcatcgatga ccactttctg cttacgctta agagcatgaa ggatttgcaa aacagtctta
    72661 aagctgcaat gtctgatctg ttgaaggtaa cctatgatgg ttcgcgtgtt cagttccttt
    72721 ttgacggcac tgagatagta acaaaggttg tggatgctga gtttccggac tacaagaagg
    72781 ttcttcccaa atcattcaag gcgagacttg ttctttctac cgaggtcttt tcagatgctg
    72841 taagaagggc atcgattgcc gcaaggctcg gatcggattc ggtcaagttt gaaatagacg
    72901 atgagcagtt taagatagtt gctagaagcc ctgatcacgg ggaatccata gagattcttg
    72961 atgcaaacaa ggaaggagac aacattgtca tagcctttaa tcccaggttc ctttctgaat
    73021 cggctaagaa gattgattcc gatactgtgg agttgaattt cgttgattcc aatagccctc
    73081 ttcaaatgaa tccagtagat gtccagggtt atacatacgt tattatgcct attagactca
    73141 tatgagaatc agggtgggaa tcggttacga cattcattcg atcaagcccg gagacagagg
    73201 tatgtatctg ggaggagtta aggtatctga tacattctat cttgtgggcc attctgacgg
    73261 agatgcccta tgtcatgcga ttgtcgatgc gattcttggg agcacttctt cgggaaacat
    73321 cggcatcagt tttccagagg atgaaaaaaa cagagatcga agaagtatag agtttctgat
    73381 ggaggtagct gagtcgataa gaggtcggtg gcgaattcta aacgttgacg cagttgtcgt
    73441 gatcgaaaag gttagactag cagggtttgt cagttcaatg actgagaatg ttgccagagc
    73501 gcttgggatc gaaagcgagc aggtgaacgt taagcccaaa tccggaaatg gcagcagccc
    73561 tggtttcgtg caagttcatg ctgtttgtct tttggagaag gtggggtaag agatgccgga
    73621 attccgttat gaagttataa acatcaaggg aaagcccgag aaaggcaagc tttctgccaa
    73681 ttctaaactt gaggccctcc agattctttc gagcagggga tttattgtca gctcgatcaa
    73741 acagtcatcg acggcacgca attatacgaa ggcctctcta tttcctgctt ctcagaagga
    73801 agtcagtatc ttctccagac agcttgcaac gatggtttct tccggtgtta ggataagaga
    73861 ctcgctgcaa gtgctttcga ctcaagttct tttttcaaga agattcagga agataatctc
    73921 ccgtgttgtt cttgatatcg agggcggtat gagttttagc gaatcgcttg agaagacggg
    73981 cgtgtttgag ccattgttta ccaatctcgt aaaagcaggc gaggcgggtg gtgttctcga
    74041 cgagtctctc gaaagggttg cagatttcta tgaggggatg gttgagcttc agaatcaggt
    74101 aaaatcggcg atggcttatc ctctattcat gatggttttc gcggtgggaa tagtagggat
    74161 gatctccttc ttcatcctcc caaatttgat aagtgctttt ggaggaaatt acgagcctga
    74221 aggaacaatg gctattcttc ttaagatgaa cgacatcctg aaaaatcatt ggccaattct
    74281 tcttgtcctg cttttagcat tgtcaatagg gggcttcttc tttttcaaga gtaagtacgg
    74341 caacattgtc aaggagactc taggcaaatt gattcctccg gtcagaaacc taagaaacat
    74401 gacggctatg gagagattca cacggacgac ggctgttctt gtagcaagcg gtgtggatct
    74461 tcctacagtt ctggaacttg caggagaggt ttctcaaagc cctagagtta tgaaagccgt
    74521 tggcaatgcg atagtcagta taaagggagg ggaaagcatc aacgccgctc ttgagaggca
    74581 aaaggtcttc cctccaatcg tggttagcat ggttgcaact ggcgaagaaa ctggaaaact
    74641 tgacgaagtc atgtttaaag tggcaaactt ctatcacatg caagtccaga atgcacttaa
    74701 gaaacttgtt tcgctcgtcg agccaataat gatattattt attgggggat ttattggttt
    74761 cctggcaata acgatttatg gtgctgtatt tgcaatggag caaagcattg gatgagtttt
    74821 accgaactat tgtgctgtct ggtcgtggtc tctctgtgta tcagcggcgt ggtacttgtg
    74881 tcatctgaca tcatagctag tctgaaaatt gttctaagca aaactgcttt tgattcgttt
    74941 attgaaagcc agcgtctcga agcagttgtt cgttcgaggc ccgttgatgt attctatgat
    75001 ttcagatcaa gaagagtatc atccacaact gggatggtat ttgaagactg tctctgggag
    75061 aaccctgaaa acttcaggat tcgttttaat ggggatggtt ctatagctat acttagcggg
    75121 tcgacaacat tgaactttgc tgatggcagt gttttgacaa tccagccggt cacggggaaa
    75181 gtcagttatt gagacttggg ggtaaattat gtttcaaagt atgattactg ccgttaattc
    75241 agacgagggt tatacggaaa tcgtcagggc tgtcgttgaa ggtcgttatt taaagcccgt
    75301 tttttctgta cttaagctgg gttcggattc gcttgaagaa gcatcacgag tattcgagga
    75361 aaacaattct cttcctgccg acgagatttt tgtgactgcc ttccacccaa aagatgttgt
    75421 ttttcagcat ctcgaagttc cacgggtcaa atcagtaaag aatctggtaa gtgtagctag
    75481 cttcaaggca gcatctcagt tttctctacc gcccgacgaa gtcaatgttg cctgcttgaa
    75541 ctcattgtct tcacttcgaa cgggtcttgt cccggccttt attatcacta agaagagagc
    75601 tctgtacgaa ttcattggag aactgacggg aaagaacttc cctgaaccta acatagttga
    75661 tgtgaaaccc ttttcgattt tcaaaattgc tcagaccagc gtctttgaag ggaataatat
    75721 cgtttttatc gttgataaga gctactcgct tctattgaca ctaagaggag aggaaattgt
    75781 aggcttgaat tacatcaatg acgggtttga tgaagtggcg agcgacttca aagaagataa
    75841 ctctctgtct ccaaacacta atatgcaacg agagtttctt ataggctcca aagcctatta
    75901 tgactctttg attgaagaag cgtcggaaag actcgagagt atgatatcgt atcagttgag
    75961 aatgtacctc acaaacactc tgtctaattc tcccaataca tctgcagccg acgaatcact
    76021 cttcaacagg ttttttgttg caggtcagtc aagcctttcg acagctgttt attcaagagc
    76081 ctttcagcaa atcctgggca ctgagactga ggtagcgagc atgcctctta agatgaagga
    76141 gacaagcggt ataacatata catcggcagg actactcatg agaggtggtg agttacttgg
    76201 tcgacgtaaa cttgtattca aggaagaggc gggtctcaag acttaagtac tctctgatag
    76261 ttctgctgat aattatcttg ctgatttttg ggtcgcgttt ctttgtgacc caggctttcg
    76321 aaatcagatt gagacaggtt tcaagaatga aagggtactt gtttgacaat accgggactt
    76381 atttgacagg gaatctgaaa gaagatatag acatgatgta tgaaaagaag caggagtaca
    76441 tcgcgataag aaataaacta agcagacagg taaatgatct ccgtaaacat gttgatttga
    76501 agtcagttga aatggatgtc ttcaggacaa tagaggccta tcttgaggac gagaacgcaa
    76561 tgaaaacaat tgcctccaga atacagttcg aaagcggggt aggaaactct ttctactatc
    76621 tcatttttga aggaaaagag atgtttttcc cgacaccgca tacgactcca acgcttctcg
    76681 caagaccaag cgtcaggggt aatttggatt ttgccgagct gtactctctt cagctagttg
    76741 atcttgaggt aggtgacctc aatgaatgag aagaacggat tcaacttcga agaaatgaaa
    76801 gcacccgcag gagcagagcc ttttgagggt agcgattcgt tttttatcca cagagactac
    76861 gcaaataagc tgaatagaag gctcagtttt cttgacaaga aaggaataaa ctcaaaactt
    76921 gccttctggt cactgattgc cttgactgtt ttgggttttc tcttcttggt tattctgtac
    76981 tcctacagca cttttgagaa cacttctctg ctcattgatg aagtaaatgg tttagaaagc
    77041 gcctatcttg aagttcagct cctttcaagg gtgagaaacg aggcacttgg ttcaatgagc
    77101 actagaagct atttgaatga tcttgaagag aaaattccga tagggtcgaa gcccggcgac
    77161 tacaatggga taattgcttc gggaaactca agggatattc tcgatctcgt agaaatgatt
    77221 gtaaatgaac ccagaatact tgtcaaaaag ctagagctaa gatctaatct tgggtttccg
    77281 attctcccgg agtccaacat tccctcgaat ctctctcttg aactgaaaat tgatctaaac
    77341 gtgactggaa tcttttaggg ggagctgaat ctatggagaa gtacgttatg gtaatagtta
    77401 tcgcgatttt ccttatcgta acaggtgggg ttattctgac atttctcctc gaacccgttg
    77461 aaattgcaga cgtagcggcc agcaggatca agattgatcc tataaacact gatagagttg
    77521 ttagtagcgt aagtttggag aatcattttc ttcctctgta tgccagtcct tctgaagtaa
    77581 tcagttccat gctagaagtc ggtgaggaag ctgaaggtgt aaggtatgtc gcatactata
    77641 gattaaacaa gattgattat gctgttcttg aatctgggca gtctcaagtt actgtaagag
    77701 tgggagaaca ggttgctccc tcctacatcg tgtatgggat tacggaattt gctgtattgc
    77761 tgaacgacac tagcacaaac agctttgttg ttgtcaagta tttcaggtcg taaactcgag
    77821 ggtgaatatg gggtgattca agtgagaaga atagcggtct tattcctggt tctgggtttc
    77881 ttctgtatcg gtttctctaa tcaactgaat tcagtcatac cttctatgga tgctgaatca
    77941 gtgacggtga ctttgatttt tatgaacgaa gtcaccgatt ataacattca gtctaatcct
    78001 tccaagacaa tatattctct ggcgctggac ggagtaagcg gccgtgatat gaatctgccg
    78061 ctaagaatgg gacccgttga gggtctctgg acccgccagg acccgaagcg actttcagtg
    78121 aatgtcgcct tgctgattcc tgcaatcgac gagccgcaag tcacaatcag ggagaacatc
    78181 gtaacgctca gatttttcag atctaacatt gacgttgaca tcgagaagtt cacaacatac
    78241 ggaatgactt tgtcaactgc catgacgtat ctcttttccg aggaaatgct agacctttcc
    78301 tacataatat ctccaagtgt gagagatgag gaggtcgtcg tcggtttttc acttgcgatg
    78361 ccagaggaca tcctcagaaa cattctcact tctcttggag acagggtcgc ttactcttat
    78421 ttgacagatg gaaccttcta cctcgggaca cctgaagaag tacagagtct ggtaaacgcg
    78481 ttctggaaaa cttatattgg tgtggagttc aaactggagg gtgaaaccgt ttctgagcag
    78541 attgagagat tgagaaagac tcttcccgtg aacagtttta ttgagtatct cccaaatgaa
    78601 gcttctctca tggttttcgg agatctccaa acccatatga gtctttctgc tgctcttaca
    78661 actaatacgg ttacgaagga atttaccatt ccgtgggaga taacagatgt ggaaggcact
    78721 cctctcaact cttatttaga ccttgcaagg aaattgaatg caacgctttt ttctgagagt
    78781 ctgacaataa taagcattcc tgaatttaac aaacttattc tttccgggaa tgattctcag
    78841 gtaaatgctc tttatgaata tctagagctg tatagagaaa atgttacatc tgaggatcta
    78901 gattcaagta aagcgagaaa aacaataacg ctaccggaca acttctttct catcgaagat
    78961 gtgctgaaac taggcagtgt tggcgggtcc ggcttgacag aaggcaatac tcttgttggc
    79021 gccatactga gtattcttca aagttacgcc ccttatgaag agatagctgt agatagagca
    79081 tttgagccta tcggaaaggt aacattcgag ttgccaaact atctagttcc agttctagaa
    79141 aagactgtgg attctttttc gggaatatct gcggaggttt cctacacgat agtgaattat
    79201 cacttaacaa tagaggaatc tgtgagattt caggtggagc gaataactgg agcactgatc
    79261 gagcccttgg gcgaaaggaa gggctacatt cttaaaggct ccaagagttc aataggaaca
    79321 gcagaatatc tcctctcact tttcactggg atcactttcg aagattactc cagcacatat
    79381 cttgaactaa aagctaatga ttcctttgag gaagtctcgg cctttctcag aaccttctac
    79441 tcggcacaag gtcttgtcca agatgagtac acagttgagc agattgcaga tagattggtc
    79501 ttcatgtatg ccccttcgga aataatggaa aaggctgttc ttgaactgga acagcacgaa
    79561 aggcagatat acagggtaac aaaagaatcc attcttgaaa tcgctcccga aatctattct
    79621 tcaggcctgg gcgagttact ccagacgatt ctcgtggaga atgaaacggc agacttcatt
    79681 cagtcggcag gattgctggt agtcagtgct tacccggaga gactcgccga gatagaaacc
    79741 attatcgaag agagaatacc tagaatcaaa gaaatgcttg agaatagaat gtctagtcag
    79801 gagaacaggt tatccctgca gatcgaagct attccaggtt gggatattga gaaattcagg
    79861 acatatctca acgactttct tggaactgag cttttttcag agattactct caccacatcg
    79921 ggaagcggct acatagtcat tgcagcggag tctgttttgg aagcaatttc cgctgaagct
    79981 gaaaaactta gaggatttga agaaccttct tacagactgg aaacttccat tccgtcactt
    80041 gctcagttcg agcttctttt gcagaggctt ggaatagccg taacaatagt tcctgtcgac
    80101 aaccagttca tgattgtagg ttcaaaagtc aatgtcgaag aggcttccag gcttgtaaga
    80161 cagcttagca gcggactacc cgttcaaggg gaagtttcgg aaagtgagac tgtttacgag
    80221 tatagattta tagacattcc ttattcggaa gtgcagggaa taagtgatct tctggacaaa
    80281 ctaaacctgt cagttgagtt cgtcaacacg ccatctggag tcgcgattgt agccactgaa
    80341 gataggctga aagaagcgat ggtcgtgatt gattcgattc tttctagaat cgagacaatc
    80401 gagacggggc ctagaagtta cagtttgata gatatagagc catccctagt cgatagcctt
    80461 gacgagatac tttcgctgtt agggtacgaa atagatcttg tagagtcacc ggcgggagtc
    80521 atagggattg gtgtagaggc gcagctcgca aaggcaaaga agatcgtcga agagattatg
    80581 aataaggagg catcaatact tcctcagcag ggagacattg aaagcgagaa atcatatctg
    80641 atcattttct cgaagcctga agtggaaatt gcctcgctca actcaatgat agatgttttc
    80701 ggtatggatc ttgttatcac gccaatctat gacaagattg tcgttgttgg agatagcgag
    80761 gatttaaagc ttttcaacgg attgtacgat cagatactta ctaccgagag cgttgaagga
    80821 gcctttgtat caagggagat tgcaggggtt cctggatggg attccgaacg tcttctatct
    80881 tatctgggcg tagttcttgg accagaaaag actgcaaata tctccttcgt ccagacggcc
    80941 aaaggataca taatcagttg ccccgagtcg atcattgaaa gagtagagaa cgaattcgag
    81001 aggctgaggg gaatcgagag tccatcctat tcgatagaaa gcaagatgcc tcagattgat
    81061 gagatcaatt cacttctttc gaggctcggc atcattgtcg atattctgga agtcgacgat
    81121 tattttgtcg ttgttggatc ggaatacaat gttgcacgag caaaagaact catatcgaaa
    81181 ttagttgagg cgatcggaga ggacccggaa tctaaagaaa ctgtatttga gctttctaca
    81241 atcgaaagca aggattccca ggtttttggt gagatcttct caagcattgg gatcgatgtt
    81301 aggttgcttg aatccccttc cggggtcctt gtagtaggta gagaagaaga ggtttcgaaa
    81361 gcaatagaaa cttccaaatc gattctccag aagagacctt ctacggcttt agcacaggct
    81421 gtgtttgaat tcgtgccggc agctttcgag gaaataggag attactcctt aatactggag
    81481 aaaatgggca tcgatgcgga gttgcttcca tctccgtcgg gtgtcatcgt agttggatcg
    81541 tctgaagagg tttctaaagc tagaacggca gttcagacaa ttcatgatca gaaacaggca
    81601 ttcgatagta ctgaagattc caagaacgtg tctaccttcg tccgaagagt tgcaggctgg
    81661 agcgacgagg tattcaccag ttatctcagc gacttcttgg gcgaagattt atattctcga
    81721 gtctctattg ctccaacagt aagtggttac atcgccgtcg gacccattga tcttcttgaa
    81781 actattgaag ccgaggttgc gcgactgaac gaaattcagg acccctactt cattgtgaag
    81841 cagggattga ctcctctcga tcagctggaa ctgcttattg aagcgctagg aataagagtc
    81901 gaaattatcc ccgttgaaga caaatacttg atcgtcggaa atcatgacag tgttgttcaa
    81961 acgggtgagt tgattgatca gctcatcgat gctatatacc cggagggaaa gcccacagag
    82021 cagactgcaa cttcgaagac attcattttc ctcccgatcg aaccagaaga tattgcgcca
    82081 ctcgagacgg ttatggagaa actcggtatt atctccgaat ttgttcagat ctcaaatgga
    82141 gtcattgcgg tagggtcgac ggaagagctt tcaagatctc ttgatgttgt cgaatcggtg
    82201 atggcccatt ctgaaagtgt aacggctgaa ctggaaatgg attacaggtt tgttgatgtt
    82261 ctgaaggact ccatagatca gcttctgccg ataattgaac gcttgagtat tggagttgaa
    82321 ctgcttgaca ctcctgccgg agtagttgcg atcggagccg aacaagattt ggatcgtgcg
    82381 gaagagttga tcaaagcaat cacctctaga gatacgaaag aagttcctct tcaagagaaa
    82441 tatgagagtt atcttcttct gcctctttca gccgggatta cagttgaatc actacaacct
    82501 gcgctagaac ttctagctta cagagtattt gcggtaagcg ttgcagacaa actgattgca
    82561 ataggcagcg aagaagacct gctgaagttc aaggcacttt acatgcagct tcaatctcag
    82621 gaagtagaca gggaagaacg gatttctctg gaaataaaaa tgatacctgg ctgggatctt
    82681 actcgtttga atgaatatct gagactcttt cttggagccg atgagtttga gaggatttcc
    82741 attgtttcat cttccacagg attcgttgct cttactccaa gagctctggc agacgttgtg
    82801 gaggctgaaa tatcaaggct gagagaattc gaagaccctg catttacagt gtttgaaaga
    82861 gtgcctcctg tagatgatct ggaagggctg ctggttagac tgggagtact cgttgatctg
    82921 gtagaaatgg gtagctcgac gatcgttatc gggtcggggg aagacgtaag acaaaccgga
    82981 ataataatag aacagcttat ggaaagtgtg aaattcgatg ttgcttcacc ggaagcaatg
    83041 gaatacagcg ttctagacat cgctcaagac gatatcggaa gcttcagtgc aattcttgaa
    83101 agcctcacga tagatgtttc ccttatttca tcgccctctg gtgccatagc gatcggctca
    83161 catcaagcag tggctagagc aaagacggtt gttgaagata ttctcagcag gagaacgact
    83221 ccgcaggttt atgaaccgac atacgccttc actgggatac aaagcgcaga tattgccatt
    83281 tatcagacga ttctggacaa acttgactcg ggagtggagc ttctgccatc cgctactgga
    83341 gttatcatga tcggtgaagg agacaaggtg caagaagcgg tcaagacagt caatgcgatt
    83401 cttgagagag aagcgttgtt tgacgaatca gaaggtcaac cagacagggt ctcaaacgtt
    83461 gcagccatcg tccctggctg gaacgatgag aaatatgcaa gctactttgc tgactttctc
    83521 ggcaaggacg acttctctag aataaccatt acaccgtctt ccagtggtta cattgttgtc
    83581 ggtccccagg aagttgtatc aaaactggtc aaagaggctt cgaggctggc tggaattgag
    83641 gatcccttct ttgttatcga gaattcgctt cccgcagtcg agcaaatgga ttcacttctt
    83701 gaacggcttg ggttgaaggt aacgattctg cctgttgaag acaaattcct cgtagttggc
    83761 aagaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    83821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg aaggtaacga
    83881 ttctgcctgt tgaagacaaa ttcctcgtag ttggcaagaa agagagcgta gaagaggctg
    83941 ttgagctaat aagacttctg agtaacggac tggaagcgcc atcaagttca agaaaatatg
    84001 atttccaaat aatgcctgca agcgagggag catttgatac tatacggcag gtattagcag
    84061 agctgaaaat tgaagcaact gcgcttagct ttggagacaa cgtcatcctt gtcggctatg
    84121 atgaggacct cgaaagtgcc gcgaaggtcg tgaattctat taatgaacga acggtaactc
    84181 tcgaaacgga taaagaactg aagtattctt ttacggaaat accgaatgat agaataaatg
    84241 agttttcagc cattctcgat agtcttgaga tggatgtgca gctactttcc tcgccatccg
    84301 gagtagtatt ggtggggacc gaagaagagt cggctaaagc taatgatgtc atactatcag
    84361 tgctttctaa ggccgaagag gatgagatgg ttgaatcaag gttcttcaat aaccctgccg
    84421 gctgggaaga tggaaaactt gtcacttacc tgagaagttt cctcggagag aaggattggg
    84481 caaaaatcag tgtgactccg gttcagtccg gttatctaat agtgggtcca actgccatat
    84541 tggacagaat tcactctgaa acggtcaggt tatcaggact ggagaagcca cactacgaaa
    84601 tagttgatac tgttccctcg attcagaatc ttcagggcat gctcgaaaaa atgggacttg
    84661 cggtaactgt agttatcgta gatgggagat cgatggtcat tggaccggaa gatgacgtta
    84721 ttcagactgt gaggattttc agcgagcttt ccgagggagt tgttttgccc aagggagagg
    84781 aacccgatgt atttaacttc gtagaaatcc ccaccgaaga agtcgaaagc tttagagcaa
    84841 tattctcgag attaggaata cgcgttgatc tactgaatgc ccccacgggc gtaatcgctg
    84901 taggtcaggg tgaagaagta gctaaggccg ttgacactat aacttctata ctttcaagga
    84961 gagaggttca ggtggttgat ggtgagaaga gctacctggt agttgaagtc gcggacggat
    85021 tcgacctggc tgcagcacag tcaattgttt cggcattcaa tataaacgtc ttcccgcttt
    85081 cggttgcagg gaagctagta gtaatcggga gcgagaacga gatcatgagg ttcgcagaaa
    85141 taagagaaga tataatcagc gaacatagag taacggtgat tgttccccgc gaggtcactc
    85201 tcgaggagct tacagggatt gtagatactc tggaaatgga gatttccgtt ctagagatca
    85261 gggacagctt cgttgtctac gggaaagaaa aagacatcga gcagatcagg gctattcttt
    85321 ccgagcttgt ttccgaaaaa ccttctgagg aagttcgcca ttcgaggata ttcaccggaa
    85381 tctcccttag caccgacttc cttaataagc tcctctccag tatgaattcc tcgctcacaa
    85441 tttacggaga gggagacaaa ttagttgctg taggtaccga agatgacatt gagatgtttg
    85501 aaggtctctt gaaagagatt ggatctgccc cgtctactga agaactgacg gcgcagttct
    85561 accccagaat tgaaggatgg acgggagaga cactctccgg attctttgag gctatgaatc
    85621 tggcggttca ggtatttcac gaaaacagtc agggatacct gttgattgga ccggaatctg
    85681 agctttccaa gctgcccgat atcatggagg gcctcgaact caagacgaag aagcttacaa
    85741 ctctctaccc tgttccttca ggtatgacat tcgaagagct gagagagata ctgatttcgg
    85801 ccgggttcaa tctttcctat acaaaacttg gacagagtat gtcgatttcc ggtcttgaag
    85861 aagacgtcga gcttgcagtt gccctgctgg acgaagtgat aagaagcaat cttggcaagg
    85921 ggatctctta taggctaatc aagcttcctg cagatttgac actggaaaag tttgagaaaa
    85981 tcatgggaga tatggcgcta ggcatatcct ctgttcaggt agatggagaa gttatgctta
    86041 ttggttccga cagtgatttg agaactgcca atgaaatagt tgagtatctc actccggaag
    86101 gtactcctat tgaggatgaa gtagtctact ctgtgattcc tatcagtgaa gagctttcaa
    86161 tgacggagat cgcagctctg gtcgaaaagc tttcgcttaa tgtagagatc cttccagtcg
    86221 gtgaagacct attcgtgatt ggaactcaaa gaaacgtaga tagcttcagg gagttagtca
    86281 attccatttc ggcgaccctc gggatagagg aagtccaaag ccccgaatac acaatcgttg
    86341 agttcgttga agagatctca ctggatcagt tcagagaatt gctagcctcc ctcaatatct
    86401 cggtaaagat tgtggaggtt ggagattatc tcgttttcat aggttcaggt gaggacaatt
    86461 tggaagccgc tgagcttttc accagatttg agacgaagga tacagaaccg gaatctgagg
    86521 agattgagtt cgaagctctt tcgttccctg aggggtttga cataaactct ctcacaactg
    86581 ttttcgaaaa gctcgatcta agagtcgaca taaacgccgt tggcaacgtc ctccttataa
    86641 ttggaacgag agaatcgata gaaaacgcag ttacaataat agaaaaactc gttcaggtat
    86701 cgacgggaga ttcaggtgaa gggaacgtct tcaaagtcct tgaatcaaag gagggagttt
    86761 cttccgagct tttgagtcag ctcttcagat tgatcggggt ggaggtagag attctggaaa
    86821 gcgaaacggt ctttgtcttc gtcggaagcg aagccgatgt agaaatggcc gtagatgtgt
    86881 atgattctct tggagagagc gccggtgtag acgaaggacc gctctcttac acaataacga
    86941 ttctcccgaa gaatcttact cccagccagc tccaaaccgc cttcacatcg attcagatac
    87001 ctgtaggtgt aatcgaggcg ggtgactatg caataatagt cgggacgaaa gcgtcacttg
    87061 ctagagcgca agagctgatt tcctctctcc gtataggaat cgatgcgagc gggacttctg
    87121 ccgacggttc cgtctcttac ataacctttg cgctgcccga gggcacggag atcaaccagt
    87181 tcgaatcaat tgccggtttg atggagctta aactgaaatt cgagcctgtt ggagagagag
    87241 tctttatgat cggaacggct gaagacatag gtgaattcga acaggttctc agtggtttgg
    87301 ttaacgaaag atcgggaatc gtcaacgagt tcagatttgt gaagaaacta tccggtgtcg
    87361 atgcagagac actgaagact tatttgttag caaaagagat tgagctttcg ggggtttttg
    87421 atgtgagtgg aggatacctc ctgataggaa gcgcagactc attggacgtt gcacaaagca
    87481 taatcgacta cttgctggat aaccagcagg tgcatttcgc atacatcgat ctaccnnnnn
    87541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    87601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaggc tgcagagatt caattgagat
    87661 cgattatcga atactctgga aggcttatcg gaataggaac gcaggtgggt ctggaaaatg
    87721 cgagagaggc tttgagtata cttgcagttg attcgaagag agagtcggtg agagtcgaaa
    87781 agagccttgt ttcagataat cagcttcagg agataatctc aaagctcagt cttgaagttg
    87841 attacatcga cctcgacaga cagtggttac ttataggcga tcccgagtcg ctgatgataa
    87901 ttactaggac gattgaagag gctgccgccg atagagagat ctcgagatat attaccgatc
    87961 tgaccgtcaa tccgcaagaa cttgcagatc ttttgagaga gtcaatcgaa ggaatcaccg
    88021 ttcagacctt cgcagatctc cagatgctgc ttatcaagtc aaagagttcg gccctgttag
    88081 acgcagccga agagatggta agagacgttc aggagtctag aaaggcgatc gatcctctcg
    88141 agaaaggcgt tgttgttaaa gattcgatgg tgagcataag ggtgactgat caagatctgg
    88201 aagcacttct gagaactgtt tctgaaaagc ttggaatatc gcttcttttc gttgacaaaa
    88261 tagtcgagaa tatcactatg tcgatagaag acctcagctg ggataaactg gtcaaggtga
    88321 tcaatgccac gaagccagtc gaaatagtca gaatcgatga catttatgct gtaaccaaga
    88381 aggagcagaa agccggagag gaaccgacgg atgtcgagct tgtctaccgc gtctatcata
    88441 atgtcgaaga gttaacgaga ctcattgagt tctatggggg agaagtcctt tccgatcccg
    88501 taaacggtta tatagtcgtc agagggcttg caaagtctag agtggacaac atcttcgatg
    88561 aaatcggttc agccctttca cagccgaaga agcaggtgag aatagaaaca aggcttgttg
    88621 atgagtccct gattgacgaa atacagcgag agatcaaaac ttctatcgga atcaacgctc
    88681 ccgagatcct catcgaaaac ggttccattg atcttgattt tggcattatc gattatctag
    88741 atatcacaca gctgatcgat gatcttgtga ataccggaac tgtgaagata gaggctgatc
    88801 tcagcgatag agacaacgat tctgacctta tatcaagtcc ttctatagtt tcgatgagtg
    88861 gtgaagaagc caagattcac attggagata cgattcctta cctcgtgcgc acaattgagt
    88921 atgtagatgg taatccagtg gagatagaga ctattgaata cctgaataca ggggtggagc
    88981 tgagaataac tcctacggta aatgatgatg ggaagatact ccttgatctt ttcattaagg
    89041 taagcgaacc ggagaaatac gttgacggga ctagaactct ttatggtgag aagactagag
    89101 aggcaaacag caggctgatt atttcagacg gaaacacact gacaattgga ggtcttgttg
    89161 caaataagga taccgttacg gtcaacaaga tgccttttct tagtgatcta ccttttattg
    89221 gaaagctgtt caccaccgag aacaagacct ctgataagag agaactggtt atcttcatca
    89281 cagcggaggt ggttgagccg tgataaggag acccgttgtt agcggtaagt tctacgctga
    89341 taacgaagag gaactcaaaa gtcagataag gaactgtttt ttccatcctg ttggaccggg
    89401 aagtcttcca catggctggg agaagaatga aggacctatc ggtctgattg tgccgcacgc
    89461 cggttatatg gctagtggtc cggtagccgt atggtcatac tggaaggcgg gcgcgctcgt
    89521 taaaccatca acgatcttga ttgttggacc aaaccacacg ggcttgggta cgaagctgtc
    89581 gttgtggctc gaaggggctt gggaaacgcc tctgggtagt gtcgagatag atcacgattt
    89641 tggcaggtca ctaatggctg agtcaaatgg aatgataatg ccagatactt ctgctcattt
    89701 atatgagcac tctattgagg tccagctacc atttctgcag ttcctttact cggattttaa
    89761 gattgttcct ttgataatga cggatcagag agtagaagtt gctttgagtc tgtcggagat
    89821 cataaagaag gagttaatgg ggcggaatga tgttttggtg attgcctctt cggatttgaa
    89881 ccactacgag gcccatgagg taacgatgga aaaggacaat gagcttttaa gcctaatttt
    89941 ggatgggaag tacagagagt cctacgaact ctcgcaagaa aagaggataa cggcttgtgg
    90001 gcttggacca attgtagcag tgagaaattc ttttgaatcg atggaagtgc tttgtagtac
    90061 aacgagcggt gaggtgatgg gagacaggta caggaccgtg ggatatgttg cggccctgct
    90121 cagctaatac tttggaaatg gtaagagaga acgcgttgcc ttcgcaacac caccaaatag
    90181 catgctataa tttcgatgaa ctggaggtgt cgtgtgaagg ctgtaatttc tttgctgata
    90241 gtatttctga tgagctcttt aggtctagct tcgttaatta tagatggaat tcactattcg
    90301 gggttcggct ctgaagataa actggtatat gatggaatca atgtggctta tgtattaagc
    90361 cagacaggga agaattctct tgagctggga ggcaatttct cctttgtttc tccagtttca
    90421 tcagaggatt tctacataaa tggtcacttc aatacattgc tcaagctctt tgccggtcaa
    90481 gcatatgatt tgacagagga gtcggctgtc ggactaagag ttgtttcaag agcgggtctg
    90541 ataacaacta acgttgataa gtttggtatt ttcttctcga ttactcccat tgcatacgtt
    90601 cgggtcggat ttctctcctt cggagcagca gtcggcctgg aactggagaa gttcttcgga
    90661 atggaaacac ttccagtttt tcacgcaggt ggtgagatta gttaccgctt ctagcggagg
    90721 tggacatgag agagttagct attgtgaagg agcttaaggg agatgttgtg ctccttgaaa
    90781 aagctcgtac agaggcctgt tctacttgcg gatcaaggag cagttgcagt atttcttcga
    90841 gcgacaaaaa gattacgata agggctttgc ataacggtgt ggaggttaat ccaggtgaca
    90901 ccgttgagat tgagatgagc aacatgagtg ctactagagt atccatgata gtttacggta
    90961 ttccgttggc agtctttctc gttactttga tagtaatgaa tatggtcttg aagtctgaag
    91021 gattggctct tgtagttgcc tttgcttcta ttgccgttgt ctatgcctta atcgcactct
    91081 acgatagaaa gaacagagag aaactgatgc caaagattgt cagaaaggtg gaactgcccg
    91141 atggattcat tgttcgatga ttcgatccgg ctgctggaat tcgattcagt tcttgctgac
    91201 atcgctcaga aggcaacttc caaatacggc aaagagagag tgaaatctct gcgacctaag
    91261 gctgaggacg cagaaattct atacacaagg tctagtgagt ttaacgaaat acttcttagg
    91321 gaaggggaac cgcctttcac ggtttttcac gatctcagcg actatattac aaaagtgaaa
    91381 cgcggattca gccttggtgg cgaagagctt ttccgaagcg ccgtgactat ggataacgtt
    91441 tgccgtttaa gagagttctt cgatcgggtc tctccagatt tccccgcagt ttatgaggtt
    91501 gccgctcttc tatcctgcga acgtggtttt ataggtgagg taagaaagaa gatttctgag
    91561 gatggtcaga tcaaggataa tgcttctcct ttactaaaag aaatcaggaa cgaactgaaa
    91621 aaccttaaca gaaacctcag gggaagactt gattcaataa tgaacagata caaagatagc
    91681 ttaactgata gtcttatttt gaccagagaa ggccgctatg tcttgccgct ttcggcgagc
    91741 aagaagaatg agcatcaagg tgttattcat ggtgcctccg gtagcggtgc gacggtatat
    91801 ttcgagcctc aggagcttat cactctaaat gattctatga ggattctgtt atccagagaa
    91861 gaggaggaaa tcagaaggat actgagagaa ttgacgactc ttttctttag cacatttgaa
    91921 aggcttgagc cttcgcttga ggcacttggc gttcttgacg gtctttacgc agcatgtaga
    91981 tactcaaaga gtcgacgggg tgtcttcata gagccgtccc cttcaagaca ttttcttctt
    92041 aaagatgcga gacatccgtt gatcgatgat gaagtggtgg taccgatcac cttcaccatg
    92101 gaagaggggg ttaacggtgt ttttattacc ggaccgaata ctggaggaaa gactgttgca
    92161 atgaagacga taggtctttc ggtcctcctg atgctatgcg gtcttcctgt tcttgcagat
    92221 gggttgtcga agattccact ctttagtaga ctattcgccg atattggaga cgagcaatcg
    92281 attgagcaga gtttgagtac attttcatca cacatatcca gaataatcag aataattaga
    92341 aacgccgatg aaaattcgct ggttttgctc gatgagttgg gtgcaggtac cgatcccgtt
    92401 gagggatcgg ctctatcaat agcaatcatt gaaagacttc tggagagatc caggctgatc
    92461 cttaccactc acttgacccc aatcaagctt tatgcaatgg agcgtcccga tgttgttaat
    92521 gccagtgttg aattcgatat atccactctt aaacctactt atcgtttatt gatgggtatt
    92581 cccggttctt caaatgctct tgaagtctcg agcaggcttg gcctcccaca agaaatagtt
    92641 gatagagccc gctcctatat cgatagtgaa gccagagatc tcgaagctgt aattgggaag
    92701 ctccatagag agagatcttt actggaagag gaaagaagag gtctcgacaa aactaaaaga
    92761 gaactagaag gaaggacaaa ggagttcgaa gaaaaattat cgatgataaa ggaaaagaga
    92821 tacagagaag tgagtaagga aatcgatgaa ctggaaaaaa agctgggatc tatcattaag
    92881 gatgtcgaga tggctataaa cctttcaagg gctcccggtg aaagagagaa agttgaagct
    92941 gtgaagagac ttaacaacct aaaaagagag gtcaggaatt ttggggcatc ttatccagaa
    93001 aaaaccgatt ctcagattcc ggaagttggc gacatagtta agcttctgga aagcggagct
    93061 gaagggatcg ttagcgaaat agatggtgac agggtagtga tagattcggg caagattacc
    93121 ctgaaagttc cgttatcacg tgtccgagtg gtcggaaagc cggtagtcga atcagaaatg
    93181 tcttcgtttg aattagatag tggaaactcg agccaggaga tagatattcg aggcaatatc
    93241 actgaggatg ttccaattct cattgatgat tttcttcaga tgctaaaagc cagtgggaag
    93301 aaacaaggct acataattca tgggaaaggg acgggcaaac ttgccgaagg tgtctgggct
    93361 taccttaggc gttcaaaagg agttaagagt ttcaggatcg gaacaccctt agaaggcgga
    93421 agcggtgtga cagttgtgga ggtttgaacc tgaatattag aacaggaatc gacatagtag
    93481 aaatctctcg tatgactgaa tctatagcac aaagaattct cggtaaagat gaaaaagtta
    93541 tctatgattc tctaggcgga gaacggagga gaatggagtt tgccgccggt cgttttgctg
    93601 ctaaagaagc ctttgtaaag gctcttggta gaaaagacat cgaattctcc aagattcagt
    93661 ttctcactga tgaaggaggg agaccaattc cgtccgatga actgaagcaa ttgataaaga
    93721 actctgacct tacagtaagc atatcccatg aacgagagta cgcggtttcg attgttattc
    93781 ttttcgggga ggatcaaaat gataacgttt gaagaagcta gaaagcgaat cgaagagttg
    93841 agaaaggaaa tagattatca tgcttacagg tattacgttt tggacgatcc cgagatccct
    93901 gattccgaat acgatagcct tcttagaaca ctcattgatc ttgaagagca gtaccctgag
    93961 cttaaaacgc ctgattcgcc aagcattaga gttggtttga aacccgttgc ttctttcaag
    94021 gaagtagttc atgaatatag actgttttct ctcgataaca catactcgga ggaggaagtg
    94081 cttcagtttg acaaaagagt aagggaagag ctccaaatcg agatagtacg ctatatgtgc
    94141 gagttgaaga tcgatggcct ttcaatctca gtgaagtacg ataatggcct tcttgtgaga
    94201 ggtgccacaa gaggaaatgg ctttgttgga gaggatgtca ccgagaatat cagggctatt
    94261 aaatctattc ccttgcgtct taggcaagat ctttcaattg aagtgagggg tgaagtctat
    94321 cttccaaagg atgtatttca cgagatcaac agagctcgtt cgaaaggtga acttcaggtc
    94381 ttcgccaatc ctcggaatgc ggcggccgga actctgagac aactcgatcc cagagaggtc
    94441 gctaagagac gattaaatgc cttcttttat catgtcgttg agccatcaaa gtttcagctg
    94501 aaaagtcaga gcgaacagct ccaattcttg aagagtatcg gcatgagagt tgagccaaac
    94561 agtgaattag ctgaaggtac agatggtgtg atagcgtttt ggaagaaatg gtcgcagtcg
    94621 aaatctagtc tcaattacgc ggtagacggc acggtagtaa aagttgacga tctccatgct
    94681 caggaggaat taggatacac gacaaaagca cctagatggg ctatagcgtt caaattccct
    94741 gccgagcagg ctatgacaaa gttgatcaag gttaccttcg gggttggcag gactggaact
    94801 ataacaccgg ttgctgagct cgaaccggtt caacttgcag gtactaccgt aaagagagca
    94861 agcatgcaca atttcgattt tattagtgag aaggacataa gactctttga cacagtaata
    94921 gtccaaaaag ctggagagat catcccgcaa atcgttagaa gtattccgga aaagaggacc
    94981 ggtaaagaaa aaaggataac cccgccttct agatgtcctg tttgtggcgg agaagtgggg
    95041 agagagaaag aagaggatgt tgctctgaag tgcctgaacc ccacctgccc agcgaaatta
    95101 agcagaagaa tacagttctt ctgttccagg gatgcgatgg atatcgaggg actgggcgag
    95161 aaactggtcg agcgaattgt tgaggcgggc cttgtgaaaa acccatcgga tctctataag
    95221 cttaacaagg atgatcttct tagtctagga gagggcattg gtgagaagac ggcaagcaat
    95281 ctgattgaag ccatccagaa gagcaggagc aatcccctct tcaagctgat aactggcctg
    95341 ggtattccag gagtgggatc aaagacatca agagatcttg cgaaccactt tggtagtctt
    95401 cgtggacttg tctctgcaga cgaaaaggaa ctgcgaaacg tttcaggcat cggtgaccag
    95461 ctggccaaag atatcaaaaa gtacttctct tctccggaag tcaaaaaaga gctcgaggag
    95521 ctattgaaat acgttaacat tcaggatgaa gattcacctg ggtcaaaacc gttgaaggga
    95581 atgaagtttg tggttacggg aacgcttccg ggatactcta gaaaggaaat acaggagaag
    95641 attatcaaac tgggtggaga agttgcttcg tcggtctcaa agaataccga tttcttgctc
    95701 ttgggtgaaa atcccggttc taaagaacag aaggcgcgcg acctcggaat caagatcatt
    95761 gatgaaagag atttcgagga gatgataaaa tcctaatgcg ttatttcgac aacaatgcca
    95821 ctaccaaaat ggaaagtgat ctcgcggaga gattcgcaaa actgtgcgtc gaagagtatg
    95881 ctaatccgaa ttccatgcac tcttttgggc taagagaggc tcggtacctc gaggaagcac
    95941 gccgaaagat tgcccgctcg ctttcttcag atcctaggga aatctatttc acttcttgcg
    96001 caacggaaag cattaactgg attctaagat ccagcgttct tttcaaagga agaaaaaaga
    96061 gaatcgttac tacatcgatc gaacacaaag cagttctgaa tactttgaaa gacttaaaag
    96121 caagttcaga cattgattat acaacggtcg gacccgagag aaacggaatt gtcaaagttg
    96181 caaagcttct cgacgaggtt gacgaagaga cttttcttgt ctccttgatg gcagtaaata
    96241 acgtaaccgg cgctattcaa ccctatgagg agattggcga agagcttagg aaacggaacg
    96301 ttctctttca tctggatgcc gttcaaacga ttgggaagat ccccattgat ttcgttagct
    96361 gcttctgtga ctacgcctct ttttctgcgc acaagtttca tggtcccaaa ggtgtcggag
    96421 tcacttatat taggcaggga gcgcccatta gaccattaat taccggtggc ggtcaggaga
    96481 ggggaatgcg ttcgggaaca cagaacgtcc ccggtacagc tatagccgct acagccttgc
    96541 agcgagccgt tgagtgcaca caagagtcat cttccaggtt gagagagtat cagcatagga
    96601 taaaagaaac cgttgaggaa ctagatggag ttgttaatac accggaaaac tctatatcca
    96661 atacagtcaa cgcttctttc gcaggaatca gatccgaggt tctggttaac gcgctttctg
    96721 aagaaggtgt ctttgttggt acttcttcgg catgttcttc tagaggagat ggaggccagt
    96781 atgttctcga ttctatggga cttgatttct cacttgcctc cagttcaatt aggatcagca
    96841 tgtcacggtt cacaaccgag gaagatgtgg cgtttttgat agaaaaactg aagaaaacgg
    96901 ttcctctttt gaaattttag ccacaaacgg ttataattga ctgtagtttg acctaaacgc
    96961 ctttgaggtg ataataattg ctcagaagca tgaccggatt tgcgagggtc gagaagaccg
    97021 ttcgcgatgt aaatgtattt gttgagttga agacagtcaa ttcaaagtac ttgaacgtgg
    97081 atgtgaacat aggagatgct ttctctgagc ttgaaatgaa tgttaccagg gttattaaag
    97141 agaatctcaa gcggggaact gtaagggcga gaatcgatat ttctctggtc aacagtgatg
    97201 atcttcttca accagatttc ggaatagcct cctctgtcta taattccttg aaatcaataa
    97261 aagagcgctt tgagcttgcg ggggacgtga ctgttgattc aatggcgagg ttcaaagaaa
    97321 ttatgcgaag caagccatcg gaagatctcg cgaagaaaat ctggagtgta attgaagaac
    97381 tcctaatgga ggcaattagt gtcctcaaca gtgatcgaga aagagaaggt gagaatctcg
    97441 cctcagcatt gaatgaatac ctgtgcgagc tcgagtccat tggaagggaa ctgcaggata
    97501 gttctaagga tatggttctc tattatcgtg actttctgaa gaagaaggta gagcagattt
    97561 ctgatagtca actcgacgat aacagactgg agcaagaggt ggcgctcctt gcagaacggg
    97621 cagatatttc tgaggaaatt gtaaggctta tctcacacat tgaatcattt agagaagtca
    97681 tggcttctga gaaggaaagc ggtgtgcagc tcgatttcat ctgtcaggaa atgcatcgtg
    97741 aactctcgac aattgcttca aagtcaaaga agctggccat aacaaatctc tcagttgaag
    97801 gaaggacgct agtaaataag attagggagc aagttcagaa catagagtag gaggtgtttc
    97861 acttgtacgg actcattaac gtcgggttcg ggaatgttat catcggagac agagtcattg
    97921 cgatagtgaa ccccgaatcg gcgccactga agaggttgaa ggaagttgcg aaggacgaag
    97981 gaaaactgat tgatgctaca tatggaagaa agaccagagc gattgtaata actgatagca
    98041 atcacataat actttctgcg attcagcccg agacgattgc ttccagattt atgcagactt
    98101 tcagcgatat cgagaagctt ctggaagata tcagagtttc tggacagaat ttcgaagaat
    98161 gaaaggtata gtctttgtga tgagcggtcc ttcaggtgcc ggaaaaacct cgatactcag
    98221 agaagtactc cgatccaaca ataatctaga cttttcagtt tcttatgcaa cgagggaaag
    98281 acggcctgag gaagttcagg gaaaggatta tttttttgtc actccggagg aattcaaacg
    98341 acttcagaaa gaagatgaat ttttagaatg ggcaaaagtc catgggaatt attatgggac
    98401 ctctagacat caggtcagca agagcgtaga tttgggcaga gatattctgc ttgatgtgga
    98461 cattcagggg gccatgtcat taatgagcaa actccgagac gccgtctata tatttgtcgc
    98521 accaccttct tacgaggagc tgatcaatcg ccttcagtca aggggaacag aagacactca
    98581 atctctaaga acaaggcttg aagatgcaaa atgggaactt gaacaggtga agaactttca
    98641 gtatctcgtt attaatgata atttaaagca ttcggtaagt caatttgaag caatcatcac
    98701 cgccgaaagg ttgcgtgttg atagaatggt cagagaaaag ggagaaagat tcttgtttga
    98761 ggagaaaaca tatgataatt aattacgatt tgcttcttaa gagaataagg cacaagtacg
    98821 ctattccagt agccgctgcc aagagagcgg aggatctgga ggacttcggt agaccgaagc
    98881 ttgatccttc taccgtaagg gctgccgggg ataagatcac gatagccctg aaggaacttg
    98941 aagaaggcta cattagaata agaaatgaag aaatgctaat gattctcgtc ccgaaggtta
    99001 agtaaactat tggtagtgtg aactcgcttc gcagataaag agattcttta tttgagttgt
    99061 tgacagggat aaagtgatta gatataatag gtatcgtgcg tcgggccaac gtagctcaat
    99121 cggaagagcg gctgatttgt aatcagcagg ttgggggttc aagtcccttc gttggctcca
    99181 taggttaact atcggtggta aggtgcccga gtggtcaaag ggggcggact gtaaatccgc
    99241 tggcggaacg ccttcggtgg ttcaaaccca ccccttacca ccattaattt tgctactgtt
    99301 gaggtgcatg caatggcaaa gaaaacaaag ggaaataagg ttcttgtaac attgaagtgt
    99361 tccgagtgtg gtactagaaa ctattacagg ttcaagaacc gccaaaaaaa gtacaaattg
    99421 gactcgagta aatactgccc aaagtgcagg aaacataccg agcacaaaga atccaagtaa
    99481 ggttgagtcg gctcaactga gtcaggagga atcccatggc caaggcaaag ttttggaagt
    99541 tcctttccga agtgaagaac gaagttaaga aggtgacatg gcctaatagg gagcagatga
    99601 tctcttctac cggcgccgtg ctcgtcatac tgattgcagt aggtgcgttt ttagcacttc
    99661 tagatgttct cttcactaat gcgatcggat cacttttgaa ttttttgaca ggcgctgctt
    99721 aatgctgttc gatgttggtg attaattgtg cgaaagaaat ggttcataat tcaaacatac
    99781 tcaggccttg aaaactctat cagagaggca attcagatta agatagagtc tttcggtttt
    99841 tcgcacatgt ttggtaaaat cctagttcca gaggaaacga agcttgatag aacgaatgtc
    99901 gcggcagaaa aatacatagt tcccgcaaac gcaaggcttc tcgttaagga caatgaagat
    99961 gttgccaaag gggatcccgt cgccgaagaa gttgagataa aggtcaagaa tgatggtaca
   100021 gtcgctgaag tcaagaatta ccgtgtgata ttcattgaaa ctgccgacag gcgttacaca
   100081 aagacttact acattcctga gagcgcaaag atagagaccg ggattaaggc gggcgcaaga
   100141 ataaggcaag ggatgcctct ggcaaagcag ggtgaatatt tctgtgagct tgacggaaag
   100201 atcgtctata cgcagaagat gaaacggatt gtagtcgaca aagagaatgg agaagaggac
   100261 gtctatctcg ttcacccgga tagctatgac gccaaagtcg caaagaaagg caaccatgtg
   100321 aagcgtggtc agatgtttgg cgagacgagg cgaatattct ctaagacgga aggtagagta
   100381 gagatctccg accttccagg acgtaaagag atcaagatct tcaaaataac caggacaagg
   100441 ctttatcccg gatatgtttt tattgaaatg ataatgaacg aggaaacatg gaatctcgtc
   100501 aagaatactc cgaacgtagt caattttgtt tcagttggcg gtcaacccgt tcaacttaag
   100561 gaaaaggaaa ttaaggctct tcttagattg gttggcattg aagagtatga agaacacatg
   100621 ggtgggccgg taaagattga agtcgaattt gacgttggcg aagttgtaag gataaactct
   100681 ggcccatttg aagatttcgt tggaaaggtg acggatctag atccggacag acaggaactt
   100741 aaggttgttg tcagcatatt tggaagagaa acaccggtta ttctcagttt atctgaggta
   100801 gaaaagattg tttgattctg agaaagagtg ggagggctaa gcccgttgac cacaaggagg
   100861 taggcaaata tggctaagaa agtgatagcc caaatcaagc tccagcttga agcgggaaag
   100921 gctacacctg cacctcctgt tggaccggct ctcggtcagc atggagtgaa tattatgggt
   100981 ttctgtaagc agttcaatgc ggaaacatcc gacaaggccg gtatggtgct tccagtaatt
   101041 atctctgtct acgcagacag atccttcagt ttcatcctca agaccccgcc agcaagcttc
   101101 ttgctactca gggctgcggg tattcagaaa ggctcgggag tccccaatcg agacaaagta
   101161 ggtaagatta gccgtgctca gcttgaggaa attgctaaga ttaagatgcc cgatctcaat
   101221 gccagaacag tcgatgccgc tgcaaagata attgcaggta cagcccgtaa tatgggcatc
   101281 gaaataatag gttgaggggg gatctgaatg ccggttagat caaagaggta cattcaggca
   101341 agaaagtccg ttgatagaaa cgtcagttat tcagtggacg agagcataga tttactgaaa
   101401 aactttcctc cgacgaaatt cgacgagaca gtcgagatgc acttgaagtt gggcatcgac
   101461 cccgcaaagt ctgatcagca ggtcagggga acgatttcat tgccaaatgg cactggaaaa
   101521 gacgtcaggg ttttggtctt cgccaggggg gaacaggctg agactgcaaa acggtgtggt
   101581 gcggattttg taggatcaga tgatcttgtt cagcagattc aaggtggttg gactgatttt
   101641 gatgttgcaa tagcaactcc ggacatgatg agagatattg gaaaacttgg aaaaatccta
   101701 ggaccacgcg gactaatgcc gtcacccaag gctggaactg tgaccgctga cgttgaagat
   101761 gccgttaatg gtttcaaagc gggaagatta gaagtgaaga acgacaagac tggaaatctt
   101821 catcttccca tagggaagaa gtcttttgac aaggaaaaac tgcgcgagaa cttcgtctct
   101881 gcactcaatc agattatgaa gatgaaaccg gctggttcca aggggagatt tgtgcagagg
   101941 gtattcctga ccacaacgat gggtgcaggt ataaaagtcg attttgcaag agaaaccgag
   102001 aaataactag agtctaaact gagatatatt ttcaatgcca gagacagtag gtatttaaga
   102061 gcgcaagcca cctaccgagg cgtgtagaag atctaccctt ctgcggcctc gttttggggc
   102121 cgcttttctg aaaggaggtg cgaaagtgct aacaagagag agaaaagaag aaatcgtcga
   102181 gagtcttaca aaagcttaca aggattcatc attgattctg ttcgcggact ataaaggcat
   102241 gaaagtagag aacataacta cttttagaga caagctctat gaaaactaca aagagagagc
   102301 tcagttcacg ataaaaaaga atacgctggt aagactcgct cttaacaagg ccggattcga
   102361 agaaagcgaa tggaaggatt cgataagcgg tacaacggca gttctaactg ttgaggatgt
   102421 agatcctatc gcggccctaa agataatcac cgacttcaat aagaataaca agactcttcc
   102481 gctaataaag gctggatatc ttgaaggaag attcttcaca ggtgaccggg cttcagacct
   102541 cgcaaagctg ccatcccgag agcagttgat cgccatggtc gtaggcggat ttgctgcccc
   102601 aattaccggt tttgtctata ctctgaatgg agtgctaaca aagttcctgt atgctttgaa
   102661 tgcgataaag gataaaaaag ctgaatgatt cacgcggagg tgtggtaaga tgacaaagga
   102721 agaacttata agcgcaataa aagaaatgac agtagcagaa ctttcagatc ttgttaaagc
   102781 ccttgaagag gaatttggag tgagcgcggc ggctccagtt gcagttgcgg cagctcctgc
   102841 aaatggcggg gcggcagcag aggaagaaga agagcagaca gaattcaagg tagttttgaa
   102901 gtcttttggt gcaaagaaga tcgatgttat caaggtagta agatcaataa ctggactcgg
   102961 actcaaggag gccaaggatc ttgtggaaaa ggctggaacg ccggagggtg ttgtaaagga
   103021 aaaccttcct aaggcggaag cagaagaact gaagaagcaa ctcgaagaag ctggcgccga
   103081 agtcgaactg aagtaattga gcaattgtct ggttacatat tgtctgtata ataaatatac
   103141 ccacatcagg gagacgctct gatgtgggtt tggtgtgctc aaacatacga gaggtgatct
   103201 aatgagaaag gccaattttg gcaaacgtga gcggtgggtc tttggaagag tgtatgaacc
   103261 tcttgaaatc ccgaatctca ttgagatcca gactcgttcc ttcgagtggt ttatcgatga
   103321 aggtcttctg agtgttctga agaagtattc ccccatcaag tcgcagatcc agcgttcgga
   103381 ccttaagaag ggcgagaaag gcttctctct ggattttgta gcaaccagaa tcggtgaggc
   103441 aaagcattcc attcaggagt gcaaagacaa gggtctcact tattcggttc cactttatgt
   103501 aacggtaaga atcactgatc tctacactgg agagatcaaa gaggaagagg ccttctttgg
   103561 atacttgcct tatatgactg ataacgcctc tttcataatc aacggtgcag aaagggtaat
   103621 tgttaatcag cttgtaaggt ctccaggcgt ttatttcgtg gatgagccta caaaggcagt
   103681 ttctggttct ttgccaatct atgtagctca cttccttccc gttaagggag cttggttcga
   103741 aattctcttg aatcctaaca agcagatcat gcaagcgagg attgacagaa gaaggaaact
   103801 aaacttcttc gtatttttga aagccctggg ttatgaaaac gaccttgacc ttctgaggct
   103861 tttcgaaaat gaatacgacg ggtctgacga agatgaattg ctcgcaaacg tggggagtat
   103921 gatcctggag gacataaaga cgcctggtgg tgatttgatt gcagaaaaag ggacgctgct
   103981 aaccgaagag attattgcca gagcaattga agcagatgtg aagaccataa agatgccgct
   104041 tccaattgca atgaaaacgt tcaatgcttt gcagaagaca tacggcgaag gtatgactga
   104101 ggacgatgct tatatagaac tcttcagaaa actgaggcct ggcgaaattc caagaataaa
   104161 tgccgcaaag agctacattg ccaatttgta tttcagccct gatcgcttcg acttttccga
   104221 tgtaggaagg tacaaggtaa acagcaggtt gaaaaaggtc taccaggact atctaaagga
   104281 agttgaagga aagaaagtct caaaagatac agattactca gaagaatcct ccattctcac
   104341 tgtacttgat gtcgttctcg cggcaagaca tctgaccgag attgaaagca ggccggagtt
   104401 gcttgacacc aaggaccatc ttggaaacaa gcgtgtgagg acagtgggcg agctcatgga
   104461 aactgagttt gagaaggctt tcataaaaat acataagctt ctggaagaga agctcacaat
   104521 atacacgtcg tttgacaaga tttctgttca gagtcttgtt aacgtaagaa ctttaatgac
   104581 tacgcttcat cagttctttg cgacaagtca gctttctcag ttcatggacc aggttaatcc
   104641 tcttgccgaa ctgactaaca agaggagact gtctgctatt ggacctggtg gacttaagag
   104701 agaacacgca agattcgagg ttcgtgacgt ccatcactcg cactacggga gaatgtgtcc
   104761 aatagaaacc cccgaaggtg ccaatatcgg gttgatgact tcattggcga cctttgcaaa
   104821 gatagataag tacggtttcc ttttgactcc gtacagaaag gttcagaagg gagccgttac
   104881 taacgatatt gtatatttag ccgcagatga agaagagcgt tacaatatcg ctcattccac
   104941 tgtagatatg gacgaaaagg gtaatatcgt cgatgaaaat gtcgagatta gatatgcagg
   105001 tgaagtgaga tatgttaaca gggaggaagt cgattatatg tcggtttcgc ccaagcagat
   105061 agtctctatc tcgacatctt tgataccctt ccttgaaaat gacgacgcga acagagccct
   105121 catggggtct aacatgcaga ggcaggccgt gcctgttgtc aaggccgagg caccaattgt
   105181 aggaaccggt gtggaaggag ttgcagcgag agactcagga tacgtggttc tttctaagca
   105241 cagaggaaag gtggttagtg tagacggaag aagaataatc gtccagagaa tagatgaaaa
   105301 gggcaagcca gaactcgacg agagagggaa gcctatagaa gacgtatata cgttgcacaa
   105361 gttcgtaaga acaaatcaag acacggcagt aaaccagaga ccaatagttt ccgttggcga
   105421 gatcgtccag acaggaactc cgattgccga cggcccatca actgaaatgg gagaacttgc
   105481 tcttggaaag aatgtgcttg tggctttcat gccctgggaa ggatacaatt tcgaagatgc
   105541 tattcttgtc aatcaggagc tattggaaga cgacaccttt acaaccatac atgtcgaaat
   105601 gtacgaaaca attgccagag acactcagct tggtccagaa gaaattactg cagatatccc
   105661 gaacgtctcg aaggaatcgc tgaaaaacct tgatgagagc ggtatagtta gggtcggagc
   105721 ttatgtgact tctcaagaca ttctagttgg caaagtcact cccaaaggcg agtctgaaac
   105781 cactcccgaa gaaaagataa tcagatcagt cttcggagac agaggaaggg acgtcaagga
   105841 ttcatccctc aaacttcctc atggcgtcga aggtagagta atagatgtaa aggtattcga
   105901 aaaggaagag gtatctgaac tgggttccgg cattaacaag cttgtgaagg ttttcgtcgg
   105961 cattagaaag ccactcgatg tgggagataa acttgccggt agacacggca ataagggtgt
   106021 cgtttcgaat attatcccga aggaggacat gccattcctt ccagacggga cgccaattca
   106081 gcttgttcta tcacctctgg gagttccatc aagaatgaac gttggtcagg ttctggaaac
   106141 ccacttagga tggcttggaa agctcactaa tcgttatttt gcaactccaa tatttgatgg
   106201 agctactgaa gaagaaatca tgaccgaact ctacgaagcg agaaagaggt ttggccttga
   106261 ggacggagac agtgaggatc tgaagaccgg taaagtcact ctgagagatg gacgaagcgg
   106321 caatcctttc gactatccta ttgttgtggg agtcatgtac atgcttaaac ttgttcacat
   106381 agcaaaggat aagatacatg ccagatctac cgggccttac tcgctcattc atcagcaacc
   106441 ccttggcggg aaagctcact tcggagggca gaggtttgga gagatggaag tctgggcgct
   106501 cgaagcttat ggagcagctc acactctgaa cgaaatgctg acaattaagt cggacgacat
   106561 caagggaaga aacgatgtct acaaagcgat acttaaaggg ataaacatcc cggaacccgg
   106621 gattcccgag agtttcaagg tccttattaa agagctacag ggactttcac tcgacgttaa
   106681 ggttttcgat caaaacaatg tcgagctgga tgtggacaaa ttatgagaat caatagaagg
   106741 gagctacccc tcaaggaggg aagcagatgc cagtaagcac atacaaaaga aaggtcaaca
   106801 aactggaaat cgatatcgcc tcaccggaac gaattaggag ctggtcaagc ggggaagtga
   106861 aaaaacccga gacaataaac tatcgtacct tcaaaccgga gagggacggg cttttctgcg
   106921 agaagatctt tggaccgacg aaagactacg agtgcacatg tggcaagtac aagggtaaga
   106981 agtatgaggg taccgtatgt gaacgctgca gcgtcagagt cgaatctaaa gaagcacgca
   107041 gaagaagaat gggccacatt gagttggccg ctcctgtgac acatgtttgg tacttgaaga
   107101 gtgcacccag tgttctttct tcgcttctga acattcctgc gaaggatctc gagaacatca
   107161 tctattatgg atcgaagagg gtaatcgaaa ggctgtatgt agttaccgac ccgaaggata
   107221 ccgacttttt cccaggtatg acgatgtacc agacggagta cgagatatac acaaagaagc
   107281 tcagcttcga agtcgagcag ggttacaagg tgaaggttcc ccacggaccg gttacaacgg
   107341 atatcgacgg gaaagtcagg atagaaagac ttgacagcag taccgagcga gagctcaact
   107401 gggtagtcgt tacaaacgaa acgggaatag aaaaaaagta ccctgtcttc gaaggctcac
   107461 ttatatacgt ccagaatgac gatgaagtcc ataagggtga tactcttgcc gacagattcc
   107521 tttttgaaga tgaagtcctt tcggcaacgg agtacaagat cttcgatgag tactatcccg
   107581 ggaaattcga agtggaaacg gatacagaga gcgataggcc aatagtcata atcactgaga
   107641 tcgacccaac tgtttctgag gagactggga gaagtatcgg tgaagtcctc atagaaaatg
   107701 aatatgaggc ctacaaagag ctctatcctg gaaaggtaac ctgtgattac ggagctgctg
   107761 cgattaagaa gttgctgcag agtatcgatc tggaagagct tcaggtagct atagaacatg
   107821 agctgaagac catccccaaa agcagtgcga gagctctgaa gcttctcaga aggcttaaat
   107881 acgtcaaaga cttcatagct tcaagcaaca aacccgagtg gatggttatg gatgtcgtcc
   107941 cggtaatccc gccggaccta cgtcctatga ttcaaataga gggcggaagg tttgccacca
   108001 ccgacctcaa cgacctttac agaagggtca ttaacaggaa caatcggctt gcgaagctac
   108061 tgcagatagg tgctcccgac ataataatca gaaatgaaaa gaggatgctt caggaagcag
   108121 tcgactccct catatacaac ggcagggtgg ggaaggccgt atccgacaaa tctggacgtc
   108181 cactgaaatc gctcaccgat ctggtgaagg gtaaaaaggg acgattcaga agaaatctgc
   108241 ttggaaagcg agtagattac tcaggtagag cggttatcgt cgtcggaccc gatctgaaga
   108301 tccacgagtg cggaattccc aagagagtgg ctatggagct atttaaacct tttgttcttt
   108361 cgaagcttct ccatggaagt gaatcgagca agacagcgag aaagctaaag aaaacaatca
   108421 tagaaaagga aatgccggaa gcctgggaag tccttgaaga tgtgataaag ggacatcctg
   108481 tccttcttaa tagagcaccg actcttcacc ggatatctat tcaggcattc acacccagac
   108541 tggttgaagg aaacgctatt cagcttcatc cgcttgtctg tgcgccattc aacgcagact
   108601 ttgacggaga ccagatggcc gtccacgttc cactatcggc cgcagcgcag gcagaagcaa
   108661 agtttctcat gctcagcaga tacaacgtga tttcccctgc cagtgggaag cctatttcga
   108721 tgcccggtaa ggacataatc gttggcgttt actatctcac gacagttgac cacgaatatg
   108781 aaaagaaaga aaaagagatc atggattacg atgcaagact ccgtgaagat gaggaaattt
   108841 ccgaagtaga agcaaaaaga aaagtgagag aaaaggctct cgaatgggtc gattggaagt
   108901 tctcatctgc tggagaggct ctactggcat acgactctga atatatctct ctccacgatc
   108961 ccattctagt acaactcgat ccctcctcag gagaactgac gctgacaacg gttggaagga
   109021 taatcttcaa tacaatagta cccgaaaaac ttagggacta catgaaaaag ttcaacaaga
   109081 aagccataaa gaagttgatc tacgactgtt tccacgaata tggaatcgat agaacggcag
   109141 accttctgga cgatatgatg tcgctcgggt tccattatgc aaccacttcc ggtctcacaa
   109201 tatccattaa cgatatagtt gtgtatcctc agaaagacaa gattatagag gctgcgcagg
   109261 agagggtaga tcagattgag aacctgtata agtcgggatt cctaactgaa gaaggaagaa
   109321 ccgaagagat aattagaatc tggtcggata ccactcagga gataatggaa aagacataca
   109381 cggagttcga gaagtatcca ttcaatccga tttacatgat ggtcgactca ggagccagag
   109441 gcaacattga ccagctcaaa cagctggccg gcatgcgagg tcttatggcc gatccatccg
   109501 ggaagactat tgaagtgcca ataaagtcga attttagaga cggtcttacc gagctcgagt
   109561 tcttcacttc tactcatggc gctagaaagg gttctgccga tacggctctc aggacctctt
   109621 ttgcaggtta ccttactaga aggctggtag atgtttcgca aacgattact gttacaacgc
   109681 ccaactgcgg gactcatgag ggaattgagg cagtagaact catggccgac ggggtaacga
   109741 tcgagaaact ggaagaattc ctcttcggaa gagttcttgc agccgatgtc atcaacccgt
   109801 tgacaggaaa agtccttaaa aacgagaaaa cgggaaagga atacttccga gatacgatga
   109861 tcagagatga agatgcagca ttcctctctg gttataaggc aaaggttgaa gtggcgcgtg
   109921 aagaggttct aagcatcgcg aaggtaacga ggctgattaa ctacacagag cttgatgaag
   109981 atattaagat taatgacaag gaatacaaga aaggaaccca tatcggcagt gaccttctgc
   110041 atcagcttaa gaactcagag attgaggaga taaaggtagt cgcatttccc tgtgtaaacc
   110101 agatctttgt aggagatccc ataaagaacg gttctaaaga aatgcttgta aagtatcaag
   110161 agaaaatcga cgagcttacg gccaagaatc ttcagaaaaa cgaaatcgag aaggtatttg
   110221 ttagacccac aataaaggta agatctgttc tgacgtgtga atccgacaac ggagtatgcg
   110281 ccatgtgtta cggaatggat ctttcaaacc atgaaatagt caatgtcgga gagtccgttg
   110341 gtgtagtcgc tgcccagtca atcggtgaac ccggcacaca gctgacgatg aggacattcc
   110401 atacgggtgg aatcgcgacc ggacaagaca taaccagagg tcttccgagg gctgaagagt
   110461 tgtttgaggc ccgaaagaaa ctcaaggatc ccgaagcgct ctttattgaa aaaggcgata
   110521 gtggtttcgt taaggatctc gtctctgatg ataagggtag acgaaaggtc tacatagaaa
   110581 caaagaccgg aggtatcagc gaatacgatc tttcctcaaa catcaaaccg aagatagaga
   110641 taggcgacaa ggtcactgag ggagaggcaa ttaccacggg aaccgttagg cctaggaaac
   110701 taatggaaaa gcttggcctt gttcagaccg ctctgtattt gcttaccgag ataaagaggg
   110761 tctatgccga acagggagtc gagattcacg acaagcactt tgagctaatc attcggcaga
   110821 tgctttctaa ggtcgaagtg acagacccag gtgacacgga ctttttgcct ggtgatctgc
   110881 tggacattgt agagattaga aagataaaca agtcgataga agaagccaac agcagagttg
   110941 gagagaacag agaatacgtg atcggcaaaa ggctggcaag aagggtcctt ggcgaagacg
   111001 aagatggaaa gatacagaag atagcggccg aagcagaacc gataagtgaa gagcttctgg
   111061 agaagattct caaaggcggt ataaagcaaa tactgatata tgacgtcgat gaggaccatt
   111121 atcagaagct cgtagacgac ttcaatcccg acacggtaat tcccgagaag aacattcaga
   111181 taatgccgaa ggatccgatt aagttcaagc gaaagcttct aaggattacg aaggcgtctc
   111241 ttgagagtga aggttggtta tctgccgcgt cgttccagca gacacctcag attctgaccg
   111301 aggcctccgt tgcaggcaag tccgacttct tacaagggtt gaaagagaac gtcattgtag
   111361 ggcaactcat acctgccgga actggtcttg atatgtacgc caatcttcag gttgaagagg
   111421 cggttgttcc gtcacagtac caggaagaag agttggcttg atttgattgt ttagcttaat
   111481 aggagcggaa agcatctaat gctttccgct tttcttttgt aatggtaaca ttgttaagga
   111541 attgaatttt gaaagtttgg gggcacattt gtctaagcct gttttggaaa taaacgactt
   111601 aaagaagacc tatgaaatta gcgaaacaaa agctttagat ggagtcgctt taactctatt
   111661 ctccggagag ataaccactc tgcttggaga aaacgcggca ggaaagacta cgcttgtgaa
   111721 ggtcatcatg gggattgaaa agcccgacag tggatcgatg gtcttcaaag gcgagaaata
   111781 ccttccatcg ggtccccact tcgcaaacga gaaaggtttg ggaatggtcc ctcagagcat
   111841 aaaagtcgtt gataagcttt cggtgattga gaatcttatt ctcggtttca gaagtaacca
   111901 aaagaaagtt aggatgaagg actacagaga gagtcttaga gaattaatga aacaatactc
   111961 tcttcagcta ccgctagaca atgaaacggc gtcgctttcc acagggcaaa gacagaagct
   112021 agagattctt agagtgctcc agaattcgcc tgatgtgata atattcgacg aacccactac
   112081 tctgatttcc aacagagaaa aagaagaact tttcgagatc atcagaagac tgagatctct
   112141 gggaaaggcg ataattttca ttactcacaa gcttcgtgaa gcttactcca tttcggacaa
   112201 gattgttgtg cttcgctctg gaaaggttgt cttttcagaa agtaggtata agacttccat
   112261 aaaagagctt caagaactcc tatacggcgg ggaattctgt gcacttcgtg ttcagtcccc
   112321 tacgtccgat gaatctctct tacgtgttca ggaagtgagt gttgaaggtt cttctaatag
   112381 tgaggccgta aaaggggtct cctttgaagc aagaagggga gaaatccttt ttgtttcagg
   112441 agttgcggga aatggtcaag aagagcttct agatggaatt ttcgggatga gaagagttct
   112501 gtccggaaga gtgattctcg acggaaaaga tataacgggt ttgaaacctt tcaaacttcg
   112561 taaacttgga ctggcagctc ttcattacga cagagacaat attgccagct gtaaaggctc
   112621 atccataagg gacaatctcc ttgtatactc aataagtaac ggggagtggg ttgttgaaga
   112681 aaagaaacat gtgttctcag caagaaaact tcttagggat tatggcgtga ccttcgggaa
   112741 aattcaagat ccgatttcct cactttcagg tggaaatctt cagaagttac tgcttgcaag
   112801 agagataagc agcgatccaa aggttcttct acttggagaa ccattcagag gacttgatgt
   112861 caagagtctc gagagcgtga aattccttct aatggaggca aagaagagaa ttgccatcgt
   112921 agctttttcg gcagatgttg acgaagcact cacaattgct gatagaatac tcatcatgtc
   112981 tgagggaaga gtcgtctttt cttctgacat caggccggga ttaagtactc ctgatctgag
   113041 aaagtatctc ttcggagcga tggaggagat agagtgattt caagacttca caaccttgga
   113101 cgatcactgc tggttatcgc aatttctttg agtgcgggtc ttcttctagt agtgttgatg
   113161 agtgacgatc ctggccatgc cgcaagggcc ttctttctct cagtcttttc taatcgtttc
   113221 tactttggca atctacttgc acattcgata ccgttaatgc ttacgggatt ggccgcatca
   113281 gttgccttca cagcttcctg ttttaatttg ggaatggaag gtcaggttta tctgggagcg
   113341 ctggcgggta cttcggtagg cattcttata tccgatcttg tacctggagc aattggaatt
   113401 gccttcatgt ttacagtcag tttcttcttt ggggcttcgg tggcaagtat ttccgctatc
   113461 cttaagatcc gaactggagt tagtgagctg atttcatctc tccttctcag caatggaata
   113521 gtgcttgtag tcgattattt tgtcgaagga ccactaaatg acagggcttc tggtcttgcg
   113581 gcatccataa gccttcctgc aaaattcaga ttcaatggtc ttatgctgcc ttctggttta
   113641 cacacaggga tcttcttctc tctttgtgct gcctttttat tgtggttctt cctattcaag
   113701 actagagagg gtttcaagat cagaatagtt gggaaaaatc gagctttcgc tttttatgct
   113761 ggattgaaaa cgaataggtt gctattttgg gctctgtttc tgagtggtgg attggcttct
   113821 acagcgggaa ttatagatgt tgttggaatt catggacgtg taatgagagg tttttcgagc
   113881 ggatatgggt ggaatggtat cgctatcgca ttgattgcaa ggaataatcc agtgatggtt
   113941 atacctgccg ctcttttttt tgcttaccta gagagcggag cacaaattgc agcgcttgaa
   114001 gcaaatatca cacctgaatt tgccagaata gttcaggccg tgatcttctt cttgataacc
   114061 gctgaagcgc ttgttcccaa acacctcggg aggaaacgat atgcttgaag ccatcatcag
   114121 ggatacgata aaaaactcca cgccgattgt cctcgcagcg agcggcggtt tactgacgga
   114181 ggtatctggg tggctaaata ttggtcttga aggcacaata ttgtcagcgg ctttttttgc
   114241 ggttgtagtt tcgtcgttca ctggaagcgt tataatcgga actcttgccg ccattctggt
   114301 ggcgatggcc ctttcttcaa ttatcttctt cgctgtaaga tacttgaagg ccaatctctt
   114361 tgttgttggc atagctgtta atcttctttc ggtgggaatt actgtcacgc ttgcttacaa
   114421 ctggttttta agcaaaggga ctgtggtctt cagcgattct ccaagacccg tagtcctttc
   114481 tttggatttc ctcaccggtt cgggttttgg ttggctcaac ggactgagtt tattcgatct
   114541 atcggcgttt gtagtacctt tgtttcttct tctgtttttc aagaaaacgt ccacaggagt
   114601 gaagttgcgt tcttgtggat tcgacgagga aagcgctttt tatagcggag ttaaagtaaa
   114661 cgacttaagg tttctcgcat acactgggag cggtattctt tcaggactag ctggggctgc
   114721 cttgtccatt ccaatggggg cctttgtttc caatatgtct ggaggaagag gttggttagc
   114781 acttgttgca gttatcatgg gaagaaagaa tcccttaata gtccttttgt ccgctttaat
   114841 gctagggttc gtcactgagc tttcagtttt cctgcaggtt gccactgaga tctctcctaa
   114901 acttcttcta tctttaccgt actttgcctc tttcattatc ctggcgatct cttatggtga
   114961 aaacaaggac aagaggttca tggactggta aagatatgtg catgatataa ttcttcaaat
   115021 tggaggtgta gttttgaagc ttatagatga acttaggtcg aagtactccg aaatcactta
   115081 tcttcatagt gctgccgctc tgataacgtg ggacatgaga acgtatattc cacccaaagg
   115141 cgctgagatg agatcagagg ttctagggta cgtctcgact ctcgcattca gaaaggcagt
   115201 ttccgaagag atgggaactt tgctgagtca actggccgaa ggaaaaattg aagaggaatt
   115261 aacagaagat gagaaagcaa tggtgagagt ggcaactaag gcttataaca gagtcaaggc
   115321 cattccaccg gacctatatc acagattctc tgttttatcg tcaaaaagtg aaaaggtatg
   115381 ggaaaggtcg agaaatgaaa acgatttcaa gagtatagcg ccttatctag aggagattat
   115441 cgatttattg agagaaatgg ccaggcttta cggttacgaa gagaaccctt atgacgcgct
   115501 tctggaaggc tatgaagagg ggatgacagc caagaagctg cgaaagataa tagaaccact
   115561 gaaatcggaa ctgattcctt ttattaggag aattgtagag aaaggcaaat ccccggatac
   115621 tggaattctt aaaggcagat tcagcaagaa agctcaggag aagctgagta gaaaggctct
   115681 gaaattaata ggatatgact ttgaagcagg tagacttgat gagacagttc atccattcac
   115741 gattggtatc ggtgttaatg atgtaagggt aaccacgaaa tacttacctg atgaattcac
   115801 accgtcgctc ttcggatcta ttcatgaggg gggccatgcg atttatgaac agggattgcc
   115861 gatttcgctc atcgatacgc ctctttacgg tgcatgttct ttgggtatcc acgaatcgca
   115921 gtcgagaatg atggagaata tggtgggaag aagtatggaa tttctatctt tcttctaccc
   115981 acagattcag aaggcatatc catcgaactt cagacatgta tctctatgtg aattctacag
   116041 agcaattaac aatgtagaac cttctctgat tagaatagag gcagatgaag tcacctacaa
   116101 cctacatata atggttagat ttgaactgga agaagcactc atcaaaggtg atctcaaagt
   116161 ctcagatctt ccggaagcat ggaattcgaa gatgaaagaa tatcttggaa ttgaacctca
   116221 aacaaacaga gatggcgttc tccaggatgt acactggccg tctggaacaa taggatactt
   116281 cccttcttac atgctaggga atctctatgc tgctcagatg tttgcaaagg caaaggagga
   116341 catccctggt ctggaaaaga ggattgaaaa gggagatgtg ctgtccctgg tagattggtt
   116401 gagggataat atccactcaa aagccaggcg ttacgaacct gaaaaacttc taaaaatagc
   116461 cactgggaag gagctggatc ccagctattt cctgaagtat gtaatggaga agtattcgaa
   116521 gatctacttc acttagtttt ggaggaacca atttcaataa ggagagcttc aatactggag
   116581 agcctttctt gagacaactt gaggctctcc tttttgtcga ccaaaaatcc aacaatctca
   116641 agcggctcag tcaagcccat gtccttccag atgaaggata agacttcagg atcaatgaga
   116701 tcatgtgcag caagtgcata aatggaatta gggagtgtgc ttttcaaatc agtaatcttc
   116761 tgaatttcag agccatgaag gaactcaaga gacttctcta ctacagcttt tccgattccc
   116821 tttatcgacg agagagctat gatatcttga cttgaaagct tccccgaagt aaggtttgat
   116881 ctgatagttt tagcggcaaa ttcgtaagtt cttattttga atggattgtc cctggctacc
   116941 ttcaaaagtc tcgacagaag atcgaattgt cttgcaagat cttctttctc cactcacttc
   117001 acctctaact atattttata gcagaaacca gggatttgtc caaatgatag gatgaatcaa
   117061 caaaatttct attctacttc tttttgcgaa gtacagcgtc gatattctct ttggcagttc
   117121 cgacgaacgc ttcgtagaat gcaattccgg ccgcttcaat tgcttgaact gcattccctc
   117181 caagatgaaa ggcaatcagt gcgcttatac ctttctttgt gagcatgttc accgccatcg
   117241 gtccggctcc gtgcgctaca ctgttgtcag gttcaatgta ttcttcactt ccagtttcgg
   117301 gatcgtatat aaggaaatac tttgccctgg caaatctatc gtccggaata ctattcatac
   117361 tcttctcttt cactggaaca gcaattttca tactcatcct ccaactccac tgatttttcg
   117421 ctttagcctt tgagcaatac gcacaattgc ttctgctgct tctgaacctt ttattgaaac
   117481 gactggctta cccataatag tagctttctc gacctgctcg tcaaaaggga tttttccaat
   117541 tagctcaatg ccgtttgctc cgcaccaaga ctcaatgaag gagcttttcg atgtgttaag
   117601 atcccacttg ttgatgacta ttccgaaatc cctcttgaag tgtcttattg tttcgactat
   117661 tctggccagg tcgtgattac cggaaactgt gggttcagct actaccagaa cgtaagtagt
   117721 accggttatt gaagatgttg cgggacaacc aattcctggg gctccatcta tgatgataac
   117781 ttccttcttt tcctcttcgg caactttgat ggcaagcttt cttacctccg cgacaagtcc
   117841 accagatgtc tcctctccgg gagtgagaag ggcatgcaca agaggaatgc cgtttgattg
   117901 ggacaagaag taatcgccag atttgttgtc tttgagtgta atagcatcag caggacatac
   117961 tgcgacacac atcccgcaac cttcacaggc ataaggatca accgaatagg gcccccctct
   118021 gatcactgca tcgaatctgc aatacttttc acaaattccg caagaaatgc acgaatcaga
   118081 gatctcagcc ttctttccac cgaagtattc atatttctca atcagcttgt tttccaatat
   118141 tagatgaagg tttggggcat ccacatcaca atcggccata acaatagaat caaacaggac
   118201 agaaagtgat cccgagagtg ttgttttccc cgttcctccc tttccgctta caattgctag
   118261 ctgaaacatc atgacacccc cgctgaagaa aggattctct ccgcaagcga tttgaatctt
   118321 gctttccaga ttggcgaagt ttcaacaaag agcttgcctt cagaataaga ctcagctata
   118381 ccgcgatcaa aagggattct ttcaagaacg gagaaattct cttcactggc gtattcatct
   118441 acaagagtat aagaaccggt atccctgttt attacaatgc caaagggaat atccatttcc
   118501 ctaacaattt ccacagcaag ttttaaatcg tgaagaccga atggcgttgg ttctgttacg
   118561 agtatagcgt agtcgacatt tctgagagtt tcaacaactg agcaggaagt ccccggaggt
   118621 gaatcgatta ttactattct atccctgttt atgtgcttct tcagtgttct tataaccttt
   118681 acaggagaag gctcgccaat ttctagtatt cccattccaa agtcaatctt tttgtttatc
   118741 tttccggtct gaacctttcc gattatcttt ggtctctcgg taatggcctt ctcgggacaa
   118801 atgaatgtgc ataaaccgca accgtgacaa agcgattcaa aaaccatgac tccagcgtca
   118861 aaaactgaga gagcgccaaa ctggcatgct tcagcacatt ttccacatcg ggtacatttt
   118921 tcgttatcta ttagggggag aagaagttct atctcctctt cctggttgat atccggctcg
   118981 aagaagagat gcacatcggg ctcttcagca tctgcatcga gaagctgcac ttcaaactcg
   119041 tcggaaattg cttcggcaag attagtggca agggtcgtct taccagtgcc acccttgcca
   119101 ctcaatactg cgataaccaa ggcttaccac catctcctga agccgtaacc catgcctctt
   119161 ccgggaccaa atcccgctcc aaaacccatt cttctcccga gaaagagcct tctcctcggg
   119221 aaaaacctgt acgaataatt tctaaccggg taagcgggcc ctaccactcg gtctctgggt
   119281 gcacagttgc ccatgcccca accggtcatt ggtccgtagc cttcaggacc tgttccatct
   119341 ctccacggca taatgtcact cctcctccgc actggttgtg ttctgaagtg tctcaagcct
   119401 tttctctatt tctctaagtt cctcattgat catgtcgagt tccttcttta ggaaagcttg
   119461 atactctctt aaatcttgac gttccatttc cggagaaatc tctacgtcgt aatcgtatgg
   119521 tccgtaataa ccatatcctc ttccggggcc aaatcccctt ccatatccca tacctcttcc
   119581 gtatcccgct ccgtaccctg ggccatatcc atagaatctg catgacccgt ctctaaacct
   119641 cattggacct gatccatatg gcatatagtc acctcctata tatgcactta tcatatatat
   119701 tatacaaatt cagatataaa atgtcaagac aagcatctcg ttgtagaatt atgctttatt
   119761 tctgatagta tctagataag gaggatcaat gggaaacagg gctcacgtat tactttttac
   119821 attaattgct cccttttatg atgttgcgtt caagtcgcag ctaaagagct atcgaaagtt
   119881 gctgaaagaa caccgatctc tgcttggaaa agggataaga agtgtcttag acattggttg
   119941 cggaactggt gcccttgcgc tggcatttag cgagctaggg tatgaagtga ctgccgtgga
   120001 tgcctctgcc gccatggtta tgattgccag gcacaatttg aggggtagtg gtgtatcggt
   120061 tgttcaaggt gatttctttg agaaacttcc ttttgaggac agcagttttg atcttcttgt
   120121 tgcctcttat gtcactcacg gtcataaacg tgaaggtaga gaaaagttct acaaggagag
   120181 tcgaagaatt tgcaataaag aggtcttgat ccatgagttt tttccgaaca gaaatccatt
   120241 gatctctact gttgagtttt tcgagagaag tgattacaga aagtttgtgc caaaggcttt
   120301 cgaagagctg aaagagtact ttcctgttgt agatagaagg aggttatctt cctctactgg
   120361 gtggtacatt tgtcgttgtg agtaagatgt tcattgagtt tgttcaggaa ggttcaagga
   120421 ttttaagccc tacggtgtcg tattgccgat gtagtttctt gagaagagga ttctgggggt
   120481 agacacaggt gaagaaattt atcccgtacc tatttatagc gcctgcgggc cttataatag
   120541 ctttctttat gctttaccca ctgttttatt catttgcaat aagccttttc gaatgggatc
   120601 tttctccaaa catgaccttt gtaggtctca ggaattattc agagattctc tcatcgagtg
   120661 aattctggga ttccatgctt tatactgctt actatattct cggcgtgctg cccttctcgc
   120721 tccttatagg atttctcata gcaaccttgt tgaacgactc cacaatgaag ggaattggct
   120781 tttttaggat gatttacttt cttccggtag tgacttcgcc aattgcagca ggcatgggtt
   120841 tcagttttct gttcagcacg gaactgggat atgtgaatca cataattgca tcgcttggag
   120901 gagaatacgt gaactggttg acaaatccag aagggatttt tcagatcctc ctgaactgga
   120961 ctggaattac acttcccaaa gtacttcagg ggccgagtgt gtctcttttt gtgataatcc
   121021 ttatgggaat atggcaaaat attggatacg caatggtcgt ttatcttgca gggcttcaga
   121081 acatcccaac gacatactat gaggcagcct cgcttagtgg agctagcaaa ttccagatgc
   121141 ttaggaagat caccgttcct ctcttatcgc caacaacatt ttttcttctg ataatgttta
   121201 gcataagttc ctttcaggtc tttggaccga taatggttat gactcccgac ggaggaccac
   121261 ttaacacaac ttcggtagtt gtttttcggc tctatcgtga agccttctct tactatcgat
   121321 tcggttatgc cgctgcaatg gcctttgttc tattcctgtt tgttatttcg ctcactgtat
   121381 ttcaggttag aacaattgag aggactgttc attatgagta aaagggtcgg tagaactgtc
   121441 aagtatgtaa ttcttgtggt gggagccatt gtaatggtct ttcccttctt ctggtctttt
   121501 atgacatcat tcaaagatct aaaggagata cttagggatc cgttttcact gatacccaaa
   121561 gagtttacaa tgaagaacta cgtaagtgtc tttaccaaag ttccatttgc taggtatctt
   121621 ttaaacagta ctgttgtagc attggcaacg actttcttgc agctcttgac ggcttccttg
   121681 gcagcattcg cttttgctag aatgaggttt cgtggcagag atgtgatatt ctatgttttt
   121741 ctggcaacta tgatgattcc acagcaggtt gtcatgattc ctcaatacct ggtcgtcatg
   121801 aaacttaaca tggacaactc ttatcttgga ttgattttgc ctcatgcctc gaccgctatt
   121861 tcgatctttt ttctgaggca gttctttcta actattccaa gagatctgga agacgcagca
   121921 acaattgacg gttgtggtcc tctacgcact ttggtcaatg tctttttgcc acttaccaaa
   121981 ccggcaatag caacaattgc tgtcttttcg ttcatgtggt cgtggaacaa ctacttttgg
   122041 ccgcttctgg taataagtga gccgaagatg aggaccgtgc agctcggtct cgcgatgttt
   122101 aaatcagaag ggggaattca gtggggagag ttcatggccg ccaccgttgt agctacactt
   122161 ccgattatga ttgcgtattt catagctcaa aggcagttcg ttaagggaat aactcttacg
   122221 ggactaaaag gatagtgaaa aggaggaaaa gatgaaaaga ataattactg tatttgcctt
   122281 gttagttttc tcgcttactg ggttgggagc cgtgacactt actttctggc actcgatgtc
   122341 cgattatcag aaaccaataa tcgataagct cgttgcggat tttaacaggt ctaatgacgg
   122401 catcgaagtg aaggcaatct ttcagggttc atacgatgag actgtaacaa agttgaaagc
   122461 tgctctgcta gcaggaaagg gtccggatat tgcacaggta aacattgaac atatccaaat
   122521 tttctccaaa gatggatcgt tacaagatct tacagatctg atttcaaatg atcccacact
   122581 cagtgcagaa gatttcgtcg aaagcttctg gcagactata attcgcgatg gtaaacctta
   122641 tgcattgccc ttcaacataa gtgctttaat gcttttctac aacaaggacc acttcgaagc
   122701 agctggatta gaccccaata aacctcccaa aaactgggaa gaactggagg agtatgccag
   122761 aaagcttaca atcagaaaat catcagattc caggccttct caatacggtc ttttgtgggg
   122821 agtagacacc atgttttacc agtttgaacc tttagtctgg caaaatggtg gagagatttt
   122881 caatgaagac atgactgagt gcatcatcaa ctctgccgaa ggaatcgaag ctttggaaac
   122941 gtggagacgc tggtttgcag agggtctttc cccagcagat ttaacaatcg atgaaggtat
   123001 ccagtcattc acaatgggta gaatctcaat ggggccaatg actagtggag gaatccgtta
   123061 tgcactggag aacatccctt gggatttagg ggtagctccg cttcctgaag gaaagctgaa
   123121 ggcaacaaca ctcggtggtg gttcgctttc catcatgtcg ggactttcgg aggagaaggt
   123181 taaggctgca tggaccttca tcaagtggat ggtctcggaa aagaacactt tgttctggta
   123241 tgaggggact ggatacatgc ctgttctcaa gtcggctatg gattctctgg agattcagct
   123301 tatctggcag aggttcccgc agttgaaggc gcctatagaa tcagtcgttt ttgcgaggcc
   123361 aagaccagta cacacgaaca ttatcgagat cagaaatatt ctatacaacg cagtggaaca
   123421 ggtaaggaaa ggcgtttcgg aacccaaacc agcttttgat gaagctgtaa tcaaagtcaa
   123481 tgagctgctg aagtgaggtg atgatatgaa gcgagtactt ctggcaacag tgctcttatt
   123541 attttcattg acagcttttg gtgcggacag aattgtgggg atagaggtta caggagttca
   123601 taaaacaaac ccgcgagtag taataaacat ctcggggcta cagattggtg aagagctgga
   123661 tatcgagaaa gtttacaggg cgaagagaaa tcttgaggat tgcgggcttt tcacagatgt
   123721 ctacctccag ctgaatgatg tcgatggcga ctacaaacta ataatttcag ttcaagagcg
   123781 caacactatc tacccgtact ttggcgagta tctggcattg ggggttggag ataagagttt
   123841 tttgggttca ggtgtcaacc tctatggagg tatcaggttc ctgagattta ccgatgaaaa
   123901 actactgggg gttcttccca aacctcaatt cttctggggc ggccttactt tgggtgcaag
   123961 caccttcagt gcctttggaa gcgggttaga tcctttcttg gaactgactc tgttcagaga
   124021 agttccctgg agggacgatt tgattcttac gctggactca tttggtgctg gaacaaccta
   124081 tcccttcgaa gacttagttg tcgggatatc ttacgcatat gaaaaggcaa cgttcgatgc
   124141 tacaccgttg aataactact cgctatcaat cctttcaggg gccttcgcgt ttggagacga
   124201 ggagaattat gaagagaaaa gagggaattt ctatggtcag actaccctct cctacgggtt
   124261 cggaaaagga atcaattacc taaatcagaa agcttcactc acttactggt atagaataat
   124321 cggtagaatt tacgtccaaa gcgatacaat ggcaggatta acttatttcg gccaggcgcc
   124381 cgtgacgaaa cagttttatc tcggcggtaa tcttgatctc aaggggtggg aaccttattc
   124441 cttcaatccg agattcttcc tgttagaaat actggaggcc ggagtccctc ttaccgagag
   124501 ttttggtatc tcctcttccg atgaaatgag cgtctttata ccgaagctct acctcatggg
   124561 agctctcggt gacaatgaaa ctcttgggat gtctgttgga atcggtttcg aatggaagac
   124621 accgcttggt gttgcggtag aacctcaatt attctttgga ggaagtggat tcaaattcta
   124681 tctagagttg aactagacga gtcgaatatt cgttattcga gcaaatagct tgaaaatgca
   124741 atagaaaagc accggccgct gtacgcggcc ggttgctctg cactagaatc tctttactct
   124801 ctgattatta ctttttgcgc atgatcagaa atcaggtcgg caaatctttt aagaacgctt
   124861 gagggatacg gcggcgtttc acacaagccg gcaactggtt tgtactgctg aactgaaacc
   124921 aacgatgctc ctccacagat tctagatagc tcttcaaaat catcttcggg aagatatgcg
   124981 gggaacattg taattcttat ctcatgattt gcgaatccct tcgctatttc tatgctgtct
   125041 tcgatctctc tcagattcac aggtgaaaag atttcgtagt ggagtgactt caaatcgatc
   125101 gcaacatagt caacgatctt aagaaactcc tgcaggattt cagcattcca gccgttcgtg
   125161 tcgatcttaa gaagggtttc tggaaaccgt gatttgagcc tccgcataaa cggtaacagt
   125221 tcattggctc tcagggttgg ctcacctccg gttatgacca ctgcgtccaa aagctttcct
   125281 cttttgtcga ggtaatcgaa aacttccgat tccgccaatt cttcctgaac ccttttcttt
   125341 aattctgaat tgtggcagta agcacaatca aaattacaac ttgaggtaaa cagtgtcagg
   125401 gctactttcc ccggatagtc cactaggctt acgctctcta ttcctgcgaa attcatctac
   125461 tctttctctc cccactcggt aagtttcccg tgtcttaaac tcttcttgct ttccagtatt
   125521 ccagttttgt acaggtcggt aataccccgt tattcttgag taaacctcag ttggctttcc
   125581 gcattcggga caggtaaagt gttctcccct tatgtacccg tggtccggac atattgaata
   125641 ggttggtgaa attgtgaagt acggaatttt gtaattctcc gctatctttc ttacaagctg
   125701 ctttgtagtt tcccagtctg aaattctctc cccaaggaag gaatgaaaga ctgtcccacc
   125761 cgtgtatttc tgttgaagct cttcctggag gtctagtgca ctgaaaatgt ctctagtgta
   125821 gtcaacggga aggtgcgagg aattcgtata atacggattc tcctttccag cagtaatgat
   125881 ctgagggaac ttttccttgt ctgtcttagc cagcctgtag gcagttgatt ctgccggagt
   125941 tgcctcaaga ttatagagat ggccagttgc ctcctgatag tccatcattt tgcttctcat
   126001 aaagtccaga accctaagtg caaactggta gccctcttct tctgaaatcg ccttttttat
   126061 ccacctcgca ttcaagctcg cttcattcat acccaccaag cctatagttg agaaatggtt
   126121 gtcaaagttc tttaagtatc tcttcgtgta tggatagaga ccttcgttat tcaatttctc
   126181 gactactgct ctcttgattt cgagactcct actcgagagg tccatcattt ttgaaagtct
   126241 tgcgaagaac tcttcttcgt tcttgctcaa atatgcgatt ctcggaaggt tgatcgttac
   126301 aactccaatc gatccagtaa attcgtctga gccgaataga cctccgcccc ttcttttcaa
   126361 ttctctcttg tcaagctgaa gcctgcagca catgcttctt acgtcatcgg gatccaaatc
   126421 agagttgatg aagttctgga aatacggggt accgtatttg ctggtaattt cgaaaagaag
   126481 ctttgcgtct tcatcttccc aatcgaagtc tttcgtaata ttataggttg gtatcggata
   126541 ctggaagccc cggccgttag catctccttc gtccaaaact tcaaggaagg cgcgatttat
   126601 catgcgcatc tcttcctgac aatctccgta tgtgaagtcc agggccttcc caccaacaat
   126661 tgcaggctgt gtcctcaatt tggtcgggac tacccagtcc agcgtgatgt ttgtaaaggg
   126721 tgcctgcgtt ccccatctcg aaggagtatt tactccgaag acaaaggact ggatgttttg
   126781 cttcacctct ttgagagaaa ggttgtcaat tttcacaaat ggcgccaaat aagtgtcgaa
   126841 agaagagaaa gcctgagcgc ctgcccattc attctggagt attccgagga agtttaccat
   126901 ctggttcacc agggttgata ggtgttttgc cggtgatgaa gaaacttttc ctgtcacccc
   126961 gcccaagcct tcttctatta attgcttaag actccagcca gcgcagtagc ccgacaacat
   127021 tgaaagatcg tgaatgtgaa tgtctccctc acggtgagca ttgccaatct gaggatcgta
   127081 tatttctctc aaccagtaat tggcagttac cgttccactg ttgtgaagaa tcagcccgcc
   127141 tattgagtag gtcacagtgg aattttcgtg aactcgccag tcaagcatgt ctagatagct
   127201 attaactgtt ttcgaaaagt caagcatcga ggaatcgagt tctctcagtt tttcacggtg
   127261 tcgcctataa agaatgtaag atttggcaac atctgtgaat ccaagacttt caagcgttct
   127321 ttctacggag tcttgaatat cttcgatgtc gattagatta tccgtggctt taggctgaaa
   127381 atcggccaca accctcaacg caacccgctc aagtatgtct tcgttataat gggtttccgt
   127441 tgcatcgaat gcttttttca tagcgttgat tatcttatct agatcgaagc ctactatggc
   127501 cccgtttctc tttctcacat taagcatcac gatacctcca taaatatgat aatgaaatat
   127561 ccccacaaat tgtgtctaaa gttgattgta ccacaatatg ttgttatttc aaaaatagac
   127621 gccctatgat tatctcagtg gggaagtttt gttattttct tgggaagtga tagcggttgt
   127681 ctaagcacga gttcctgaga gactttcaga attcaaagga acttaaggtt tgacgaaagt
   127741 gaaatgtgtt aaatgttggg tttctttatg aaacgggtaa cgctatttgt accaattcta
   127801 tttaacatcg caacgaaggc ctcggcagtt gccagtgcgt cacctaatgc tctatgtctc
   127861 gattcaggta taatacccag tcttgccaaa agaatgtcaa ggttgtttct cccatatctg
   127921 aagacggcct ttgagacttc tattgtgtcg ataaattggt tgcgaagctt acccagtcca
   127981 catttatcac catagaagtt gataaaggaa agatcaaaag gggcgttgtg aattattagc
   128041 gttgcatctc cagcaaaaga atagaagcct ctgagaacat cgcaaactgt tgacttgcct
   128101 ttcacggtca agttgcttat tccagtaagg gcagttattg ctattggaat tatgcattcg
   128161 ggatcaatga gagaatcaaa gaagtcatct ctgcatagtt cccagttctt cacacgaacg
   128221 ccagccacct cgatgagttt ggctcctcgg tatggattca tcccggttgt ctctgtgtct
   128281 atcactacaa attccaaatg acttccccct cttctcattc tataacgaaa ggatctcttt
   128341 cggaaaggat gtcaagttgg aagtgcgaag aaatcagaag atcctttacg ctttcttccc
   128401 agtttgtagg cggtcaatag agtataattt ctatgaggtg atccacagtg gatgacaaag
   128461 taaaccattc cgaagaggaa ataacggaac gctataggaa ggcccttgaa tcagcgattc
   128521 agaaagcacc gatcagatcg ggcgagaggc tttatcttac agatctctgg gccattacct
   128581 ctattcccga agaagtcatc gtagagactc tgggttcttc cgaagtgaag ttacccgaag
   128641 gagtatcatg cattgttgac gatagaagga aaaagaagag gataatatat gggaaacggg
   128701 agccaaacga aaagggtagc aatcctaagc agtccagaat acaaaaagtg cagggagaag
   128761 atcagtgagg ggctagatct gatcggtttc gacgagactc tccggggaaa gaaggttctt
   128821 ctaaaagtca atcttctatc ggcaagatca ccggaaagtt gtgtaaccac gaaccctttg
   128881 tttgttagag cagttgcaga attatttctt ggaagaggag cggaggtgag cgttggggat
   128941 tctcccgcta gcgtaagcac ttctgttgcg ggatatgctt ctggaatctc acaagtgtgc
   129001 aaggagttgg gaatacctct gctggatttt gatgaccccg ttcaaatcgt ctttgacgaa
   129061 ggaacgtaca ggagtttcct tatttctaaa ccagttcttg aaactgatct cctgataaat
   129121 ctccctaagc tcaagactca tgcactaact cgggttacac taggggtgaa gaaccttttt
   129181 ggatgtgttc ctggtgcaaa gaaacagggc tggcatttca gagtcagaaa catgaaggag
   129241 ttttcgaaga tgctgttaga tctcgccgtt catctgaggg cagatctaac cgtaattgat
   129301 ggtatatatg ggatggacgg taacggtccc tcgaacggta ggattatcaa accaggaata
   129361 attgggatgt caagagatgt ttttgcactg gacgatgcta tagcggaatt atttggcatc
   129421 aaccattcaa aagttccgat actgcaaata gcgagagaca gaggcctggt cggtgactat
   129481 gatgtagttg gtgacgaagt acatcctgac aagctccttc ttcccgaaac taacttcatt
   129541 ggtgtctatg gcgcagctat actgagaaga atagttacga aatttccgaa gatagataga
   129601 gaaagatgca ggagctgtcg tgtctgcgaa aacgcgtgtc cagcaaaagc tattgacata
   129661 aacagatttg ccatagacta caacaagtgc ataacatgtt acgtctgtca cgagctttgt
   129721 cccgaagatg ctattgtttt tacgagacgt ctttttaaat aggaggggtt tgatgaatta
   129781 cgttcttgct atcgatcagg gaacaagtag cagcaaagcg gtcctttttg atcaagagct
   129841 gtatcaagtt gcggttgcgc aggaggaatt caagcagatc tatcctaaac cgggatgggt
   129901 ggaacatgat cctagagaca ttattctcag tactatgagc acaattgaga gagtggtctc
   129961 agatgcaaga atttcattca gggagatagc ggctataggt atcacaaatc agagggagac
   130021 agttgttgcc tgggatgctc tcacggatca accgctttac aatgccatag tctggcaaga
   130081 cagaagaact gccggtagat gtaaagagat agaaagctct tatggagagt taattagacg
   130141 gaagactgga ctcaaaccgg atccatattt ttccgccaca aagatggaat ggctgctaga
   130201 gaatgtgcct gccgttagag atgcagcaaa taggggaaga ttgagattcg gaacgataga
   130261 ctcgtggctt gtatggaatc taacgaggga gaatcttcat gtgtgtgatt tctctaatgc
   130321 gtcaagaaca atgctattta atatagaaga gctgaaatgg gataatgatt tgctcaatct
   130381 ctttggtgtg aaagaggaat atctgccaac agtcgttgag tcctgtaagc tcctcggccc
   130441 ttcgatatat ggtccgagaa taggtggaat ggctggagat cagcaggctt cgctctttgg
   130501 gcaaactgca tttcagactg gcgacgcaaa atgcacttac ggaacgggct cgttcatttt
   130561 gatgaacaca gggaagaagc caaagatatc tgaacacggt ctgctaacca ctataggatg
   130621 gaagtccaag gaggaaattg tttacgctgt tgaagggtcg atatttgcta ctggagcttt
   130681 gataagctgg ctgaaggacg gacttggaat aatcgacgct ccttccgaaa ccgagtctct
   130741 tgccagagaa gtcgccgata atggtggtgt gttcttttcc ggagcgctta caggtcttgg
   130801 agcaccctac tggaattctt ccgccagggg tatgatgatc ggtctaacta gaggaacgag
   130861 gaaggctcat attgtaagag cggtccttga atacattgcc ttcaggacaa gggaaatact
   130921 agatttgatg gaagaggaaa cgggtattag attgaataag ctactcgtgg atggaggggt
   130981 aacccgaaac aatcttctca tggaaatgca ggcaaatgta ttgaacattc ccgtcgacag
   131041 atctctcata aaggagacaa cagcgcttgg agcggcaatg cttgcaggaa tatgcacagg
   131101 cttatgggat ctcgagagtc ttaagaataa aagaatcagc gaggtggtgt ttgaaccatc
   131161 gggtgacaga atggaggaag agttcagaag gtggaaagag gcgctgaaga gatctatgaa
   131221 ttgggcgaat tagatctgag ggaattcgag agagttgcta agaaaatagg agagatgctc
   131281 gaaggaggag agacggttct cctctttggc gaccttggaa gcgggaagac aacttttgtt
   131341 aagtcgatgg ccgatggatt gggtatcgat cgcgactacg ttaggagtcc tacctttaat
   131401 attgtcaact cctacccgcg acgatcatca agaaaagaga acaatgaaaa cccggtgcaa
   131461 gtggagaggc ctggtcttat tcatgtagac ctttacaggg ttgatagcga agaagagatg
   131521 ttagatcttg cacttcacga cttggtggac ctggacactg ttgtggcggt ggagtggcct
   131581 gagctttatg tcaagtatgt ctctcaacct tacctgattg taaggttgga gcactgtgac
   131641 gagtcgaaac gaagcatgag aatagagcct catggtcgta aaatgacgac acttttgaat
   131701 cgtctggtta tgataattaa tgaagatgaa agaagggaag gtgaatagat ggctcagaaa
   131761 tttgaattac tggaaaaggc ggccgttggt agcgaacgga agatagaaat tccggaaaag
   131821 ctgccagtga ttcccacgag aacaaacatg cttgtctatc cttcggcagt tatgccgttg
   131881 tatgtggggc gtgaaaagtc gctggccgct ctggaagaat caattggtaa gttcaatcag
   131941 atggtctttc tcgtatctca aagggatatt acgaaagagg acccggaaat tgaagagctt
   132001 ttcgagatag gcacaattgc cagaattgtt cagctcatga agatgcccga cggaaattac
   132061 aagatacttg ttgaaggctt gacaagagcg aagttggttt cagttgaaga aaaggaaaat
   132121 tcactgattg tcgtggtcga aaaactaaag ggaaagggta gaaagagcaa gatgcttcag
   132181 gcgctagtaa gaaaggtgaa agaacttgcg cttaggtatg tctcaatgag caggcgtttt
   132241 cccgacgaag caataatggc tctagaagac acatctgatg cggacaagtt tggagatttc
   132301 gtttcctcga tgatgccttt ttcgcttgaa gagaaacagc ggcttctcga ggagattgaa
   132361 gcgaaggatc gtctcaacac cctgatggaa ctactcacaa gggagataga aattctctct
   132421 ctggaagaag agctggataa aagggttaag gaaaagatcg agcaaggaca gaaggaatac
   132481 tatctcaggg aaaaaatgag agctattcaa gaggaattag agggcgaaga agatgaggaa
   132541 ataaaagagc taaagaacaa ggcagagtca ggggagcttc cgcaggaagt taaagaaaaa
   132601 gcggaacagg agatatcacg acttgagaag atgtctcctt actctccgga agcgacagtt
   132661 gtaagaactt atcttgactg gttgttgaat cttccttggc aagaagagac cgacgacgaa
   132721 atagtgatca gagatgttag aaagactctt gacagcaacc actatggact cgaggatgtt
   132781 aaggaaagaa tactcgagtt tcttgctgcc aggagatttt caaagaatct cagggctcca
   132841 attctatgtc ttgtaggtcc tccgggggtt ggaaagacct cacttggaag gtcggtcgct
   132901 gaagcaatgg gcaggaagtt cggcagaatc tctcttggag ggatgagaga cgaggcggaa
   132961 ataaggggcc ataggcgtac ctacgttggg gcgcttccag gacgaataat gcagatgata
   133021 aggaagctga agacaagaaa tccggtaatt gtccttgacg aagtcgataa aatggggatc
   133081 agctttcagg gtgatccggc ctccgctctt cttgaagtgc ttgatccaga acagaacaac
   133141 aattttaccg atcacttttt ggaagtgccc tttgatcttt cgagagtgtt gttcataacc
   133201 accgctaacg ttctttattc aattcctgct gcgctcaagg acaggatgga gataattgag
   133261 attccaggtt acacggaatc ggagaagtat cacatagcaa gggatcacat tcttccaaag
   133321 ctattggaag agtacaacat ggattcaagc aaattcagga taacgcctgc ggcagtgagg
   133381 gatgtgataa gagattatac aagagaggca ggagtcagag agctcgatag aaatcttgcg
   133441 aagataatca gaaaagcgac gcttaagatt gcagaaggca gcgattcgat ctctgttggc
   133501 gtgaatgaac tcggtgagtt tctcggggcg ccgcttttca aagagagcga tttcagaaag
   133561 catcccgagg ttggtgtcgt gactggtctg gcctggactt cagtgggagg agaaattatg
   133621 tacatagagg tcttgcccgt tgcaggaaag ggaaagacga tcattacggg tcagttgggc
   133681 gatgttatga aggagtcggc acagatagcg gcatcgctgg ccagaaagct ctgcgatgat
   133741 gagaggttct cagaggtttt cgaaaagaaa gacttccaca ttcatgttcc ggaaggagcc
   133801 gttccaaaag atggccccag tgcaggaata actctcacca cggcgattgt ctctgccgtt
   133861 actggaagaa gggtgagaaa tgatatcgca atgacggggg agattactct tcgtggtaaa
   133921 gtactgccga ttggaggtct gaaagaaaaa ctgatggcag cttatcgggc aaggatgaaa
   133981 aaggttctaa tcccgctggc caacaaacga gatctcgaaa aagtccccga tgaaataaag
   134041 tcaaaattgg agttcgtctt tgtcgaggac atccgagagg tgctgaagga ggcgctgatc
   134101 cccggtgacg gtaaagagca ctgagctagt gaggacggtt ttttctaagg gtgaataccc
   134161 ccctcctctt agaagagaaa tcgcctttgc cggtagatct aacgtcggta agtcttctct
   134221 ccttaattcg ttgtttggaa ggaagctggc gaagacgagc tcgactcccg gaaagaccag
   134281 gtcgatcaac ttctatggcg taaatcgaga gatctacttt gtggacctgc ccggttacgg
   134341 gtatgcaaaa gcctcaatga cggagagaga gaaatggaag aagttgatta tcgattattt
   134401 tgaatcaaga gaagagctct ctctcgtcgt agttcttgtc gatataaggc atccattgca
   134461 gccggccgat attcagatgc tcgaatgggt tacatactat gctatacctt ttattctagt
   134521 ccttactaag gccgacaagc tctcaaggag ccagcagata atagccgaga agtcaattaa
   134581 agctgaattg gcttcactgg gagtctaccc gcctgtattt gtcgtatcgg cggtgaaaaa
   134641 gcaggggatt gagaagttat tgattgcgct tttatagata ttcaggaaaa ggaaaaaagg
   134701 cgggggttcc ccgccttttg ttttaattgc ctgttatttg aagacagcat aaagaataac
   134761 aagcgggctc atcaaagagg caacgagcga gataaccgtt attagcatgt taagcgaggg
   134821 accggcagtg tctttgaagg gatctccgac ggtgtcacca ttgacagccg ctttgtgtgc
   134881 gtcggaaccc tttccacaga aggctccatc cccggtaaat ttctttgagt tgccgcacat
   134941 ttctccactg ttagatatca agagcgcgtc ggacaatccg gagaggatcg caccacccag
   135001 gaaaccgccg agggcatgta atccacctat gaatgcagca ggaagcgtaa tcccaatcgc
   135061 tattagactc ggaagcatta gctcctttag agttccggtt gttcatatct ctacgcatcg
   135121 agcatagtcc gacttggccg ttcgctctca gatctttcat ttctgtcagg agaggctctc
   135181 tcgaatgaaa tccctgaact tgtcggcact accacggggt cttagttcta gattttcccg
   135241 cgccgtttcg taaagcccga acttcatcga ataaccttcc ttccattcga aattatccat
   135301 taaagaccag tagatgtacc caaacactcg ggcaccctta tccattgcgc ttctaaggct
   135361 ttttagggcc gtttctatgt attcccatct tctcttgtct gtaccgtcgg cgataccatt
   135421 ttctgtgatt atgatcggta attcatacct cttgtaggcc ttcagtacta cgtcttcaat
   135481 tccctgcgga aagaattcgt aacccatctc tgtcttttca aattcatctc cgacaacgat
   135541 ctcgggaagc ggttttgcat acttagcaaa cattcgggtg tagtaattta ctccgaggta
   135601 atctagtttt gaggatatcc ccgatactgc ttctccggtt ccgagaggtt taaccatctt
   135661 tccattcttc aggctgtcca gaaagctgta gttgtaaacc ctgtcaagat acttggacac
   135721 aattctgtct ccagggtgga acgttctcag aggaaagaaa ggaatcatgt ttactgccac
   135781 gcccaccata gagaggggat tggactcttt tatgacatca taagcctcac tatgagcgta
   135841 aagtagattt gcaagaaccc tcatcgctct ccccatgtct tttatttccg ggggccattc
   135901 tcccatgagg tatgacatta ccgcataaac attcggctcg tttattgtca cccagtaatg
   135961 aacattctca cccatagatg agacgattgt cgagacgaaa cggcgaaagt aaggtagatt
   136021 ttctctgtcc tcccaacccc caatttcaga gaaccactgg ggaagcacga aatgattgag
   136081 agtgagaatg ggctgtattc cgttttcaat tagcatcgtt acgaaatctt tatatctttc
   136141 tattacactg tcttcaaatc tgttgacttt gggttcgatt ctcgcccact caactgaaaa
   136201 cctgtacgca ttcacaccca aagcctttat agcttccaaa tctctttcga gatgctccca
   136261 actcccacaa gcgacatcgg atgtgtctcc atttttcacc ttaccttgat gttcccatgc
   136321 gtaccaatca gaaaatacat tgcccccttc tatttgatgg ccggccgttg caacacccca
   136381 catgaaaccg tctggaaacg aaagcatatc ggaaacctcc taagtatgtt atgtgtatac
   136441 tcatatacaa ttataatcat gaaggtgggc aaagtaaaaa ctactggaaa gatgaggttg
   136501 gtcgcatgaa gatttctatt gttggcaatg tgaacataga cctaagcgca ttcataagtg
   136561 aaagcgggaa taatgaagaa aacaggatca gcgagatgag ctttgccatc ggaggaagcg
   136621 cctcgaacac atcaataatg ttaagcaggc taaggaagag catctatctg caaggtgcgg
   136681 ttgggagcga cttttttgga gagatggcgc ttgaattgct gaaaagagaa ggcctcaaca
   136741 tcgattatct tttaaagctg aatggaaaga cgggcttctg tttttcagcg atctctaaag
   136801 atggacaaag gcatctcttc acgtacaggg gagttaacga aaaagcgtac ccgattgaag
   136861 atgggttcga cttctaccac tttggaggtg taactcccga tcagattgaa aggtttgtag
   136921 gcaaaactgg cgaagtaagg ttttcataca accctggagg catcgtcact tttgagaaga
   136981 caaatggagt tgcggctatt gcagcaaggt gtgaagtgct gttcgttaat gagagcgagt
   137041 ggaatcatct tgaacattcg cttaccgtag agccaaagga aattgtcgta acactaggag
   137101 cgagaggatc gaaaatcaag aacggcccag aagaagctgc tcttcctgcg aaggtaattg
   137161 atacaacggg cgccggagac tctttcattg cagggttctt gcacgcatat ctttcaggaa
   137221 aagatccggc acagtgcttg aaactgggaa atctacttgc atccatggta gtatcaaggc
   137281 cgggagcaag tcctgctttc acttacaatg aagtaagtga tctcgcaaat aggtatgacg
   137341 tatacttgca ttagttgtat atactttaat aatatactat actgaatatg atgaaagtaa
   137401 ctgtatatat atttgcatag gttatgtatg gatccgtgtt gttctataat agtttatata
   137461 aaatattttg tgtatataaa gcttagatca aatatgaacg cttttgtgat agtatatctg
   137521 tagaggtgat tcaatgccag cacaaattgc atcggttgat gttgtaagaa tacttggtaa
   137581 gctgagtgac gatggagggg agcaatcaag tagaaataga ttcattcgcg agtatctttc
   137641 aaagtacact tctcccgaat ccatcgtcag tgacatcgag gggctacttg aagcctatcc
   137701 tacatttgat ggcgattctg agagtctcgg aagagcctac gccgatcttg ttaataggct
   137761 tgccgaacta tcgggtttct ccgttgacta tgtgaagtac tccggaagag agaagataac
   137821 ggggatctgg aaggtgggtg aggctattca gatcaaagtc tcggcgatta tttgtactga
   137881 tttcgaagag gccaagggag ttgcgagaaa cgctctccta atccttcccg aagcgatcga
   137941 tctcgggaga ccatttgtta cagttgggcg tcttggtatg ttgttttcaa tgatcgagca
   138001 aatcgagctt ccggttagtt cagtcgcgtc gatacttgtc acagatgata actacgacaa
   138061 aagaatatca tcatttatgg agttaatgct attagctttc aaaaaggagc tggcaagaaa
   138121 accccagata tcgcttcaaa aagagtggtc taaagaggaa cttgaagaat tcattctgga
   138181 gagacttaag ctgcccacta tagggatgct gttttcctta ctcgattcac ccatgttgag
   138241 tgaagacttg gtagacagaa taaacagttt cctggaggaa aacgagagcg aactggtgaa
   138301 aggacctatg gcactgggtg ccataatggg tgctctttcg aaacactact ccgggatcaa
   138361 agaagagctt gtattgcgcg agggaaagag atatcgactg gccgacaaat acagacctgt
   138421 aatatccgaa atcaaagcgc gctttgaaag cggtgttcag gcacgcacgc gttaaatggc
   138481 tcatattgct tgtttcttct ggcaagaata taaatagata cggaagtgaa gaaaaattca
   138541 acacagagca ttttaagagg tatcacgctg taactcctat tgaatggagc ttacacgcgt
   138601 agcattgaag aaagccatgt gctataatac gaaatatgag tcataatgac tattatgact
   138661 catatttatg aaaatctggg ggttgaaaat ggacttttcc gaatcagttg agagattcaa
   138721 agagaacatg gaatttgtga gaaatatgtc ttccaacaca atatccgctt atctttccga
   138781 tctacatcat ttcgagctct ttcttaatga gcgtggaatt gattacaagt cggtgaagag
   138841 aaaagacatc gagctgttcg ttaaagagta ttctcaaggg aagtattcaa ggaagagacc
   138901 ttctgcaact actgttgcaa gaaacctttc gacgatacga tcattctaca catttctgta
   138961 catcagcgga acggtaggca aggttcccac ggagctgatc aagaatccaa agacgcgccg
   139021 cagaatccct gattacataa cccatgaaga ggttatggag atcctctctt ccttcagaga
   139081 taccaaccta ggtaaaagaa acaaggctat agttgcaaca atgtacttct gcggattgag
   139141 ggtaagcgaa gtgtgcaaat tgaggcttgg ggatctcaga atgggcaatt cgccggcggt
   139201 gagaatcatg agcggaaagg ggaacagaga tagagaagta ccgatgaacg atcaggttct
   139261 ctccattatg aaggactatc tcgaaataag aagagagttt cccgtgagtg agtatgaaga
   139321 ccacgtcttt atagggacac ggggagagcc aatggcaaga aacgtaattc caaaagtact
   139381 aaatacacaa gtaaaatatg tatatcctga aaaacgagta catccacatt tatttcgaca
   139441 tagttttgct acacaacttc tacaaaaagg tgcaagtata aagactgtac aagaattgct
   139501 gggacatgca aatcttagta ctactagtat atatttgcat attacagata aagagaagag
   139561 agcggctgta caattactgt cggactgagt tgcaagtgaa tattgtggag atgttgtatg
   139621 atagatattc acaatcacac tacgtattcc gatggcagga ataccgtgga agaggtaata
   139681 caatcggcat tgaaggaagg tcttcagata gtcggaatct ccgatcactt tgatccatat
   139741 gtcgctcaag aggtctgtct tcagcccgaa gaagtaggcg tgtatcgaaa agagataaga
   139801 actttaagcg agaagtatcc agtaaaggtt cttgccgggc ttgagcttgg cctgcaaacg
   139861 gaaggaattc tctggcccgg tgaagagatg gactattaca tttactctgt tcacaccgtt
   139921 ccagggagac ccgatctcaa gacccttgaa aacccgtggg atatatacct tcaagaggct
   139981 atcagtgcgg tagatctcat tgacagacct ggctttctgg gccatctgga ttttttaagg
   140041 aggcatatcc cgaaagcaaa gcctcccaaa cctgggcagc ttatggatac gcttctgatg
   140101 aagcttgtat cgaatcacgt tggactcgag ctgaacacct ctggttggat gtacggaatt
   140161 ggggaccctt ctccccagcc atgggtaata gagagatata tagacctggg gggccgcttt
   140221 gttacggtag gttcagactc gcacagggcc tcacagatcg gaaatttcac tgtgaaagcg
   140281 ctttctttgc ttaggaaaat ggggattaaa gaagtgtttt attgtgagaa tggtagttat
   140341 attcccgttc cgataatgga ggcaaaatga gcgaaaagag cttcgaaggt ttttccggcg
   140401 agacactttc atttttgata gatctcggga ttaacaacaa ccgatcatgg atggaaagaa
   140461 acagggatcg ataccgtata gaactgctgg agccattcag agctcttgta aagcagctag
   140521 gcccttccat ggagagaatc gatccattta tagatgtcag accacaggtg ggaaagacta
   140581 ttagcaggat cagcagagat gcgagacgta acaagggaaa accaccatac cgaactaata
   140641 tgtggttcac tttcagagtt ccaggtcccg actggaaaga ctctcccggg ttctttttcg
   140701 agctttttcc agactggtac cggtacggaa tgggtttcta tctgccttcc cgggcgacaa
   140761 tggttaagct ttctgaagag atcgagaaaa acccggtaag gtttgttgaa aagacgaagg
   140821 ttctgagaga tagagaattt tctgtcttcg gtgagaaata caagagaagg aggaatagga
   140881 agggagtgtc tgacagcctt tccgaatggc ttcagtataa agatttctac atcgcctcta
   140941 atcacgaaat cgatgagatt cttctatcct ccagacttgt cggtcacctt gaggcggcct
   141001 tttccgcgct atcggagctt tacgaatact tctgggagtt gaaaatgaga gatgcctaaa
   141061 gcttacagta gggagcatta tctaaagatt ctcgcaaaga tgcttcaaga ttctctttga
   141121 tgcagtaccg tctccaaacg ggttttttgc gtttgccatt cgcctgtatt catcagaatc
   141181 tcttagaagt ttgactgtct ctttgaagac gaaatctctg tctgttccaa cgagctttgc
   141241 ggttcctgcc tttatggctt ccggtctttc cgtcgttttt cttagcacca aagttggttt
   141301 tcccagagct ggagcctctt cctgaatccc accggagtcg gtaagtatta tcctcgcctt
   141361 ttccatcaac gaaacgaaag gcagataatc tagtggatct gttagcacga ttctactgtg
   141421 cttttccagc tcaggataaa caacctctct tacggcggga ttcaagtgaa cgggaaatac
   141481 aactttcatc ttcggaaatt ccttaagtat gtcctttatt gctttcatga cctgtcgcat
   141541 cggttcgccc cagttttctc ttctatgcat ggttaccagt atgtactcat cttcctctac
   141601 accgagtgcc tctctgaaac tcgaagtcag tctagatcga ttcttcgtta cccagagcaa
   141661 ggcatcaatc acggtgttgc cagttaccag tagtttgttt tcaggaactc cttctttcag
   141721 aaggttgttc ttcgcatttt cagtgggggg atagtgaaat gatgagatga ctccgtttag
   141781 ccttctgtta atctcttcag gaaaggggtc atatacgttt tcggttctga gtcctgcttc
   141841 aacgtgacct acgggaatct tgtgataaaa cgagacaagt gctcctgcaa atgtggaagt
   141901 tgtgtcgccc tgaaccagag tcgcatcgaa gacgttctct gagtaaatct cttcgagttt
   141961 gcttatcaac cttgaagtaa ggcctgccag tgactgtctc tcctgcatta tgtttaagtc
   142021 aaaatccgct tctatgccaa ataaggataa aacttgatcg agcatctctc tgtgctgtgc
   142081 cgttgctata actaccggtc taattggtga attcttcaga gcaagaaaca caggggccat
   142141 cttcacagct tcaggtctcg ttccgaaaat tagcgctacc tttttcatct tcttcagccg
   142201 actggccgtt ccctccttct ttccattcgc aaaagccatg ttcagtatat cacagtttcc
   142261 cgactcaaca tcttagagag tatcgacgag cataagctgc tcaccgacac attccatgta
   142321 ttataatctt gtctggaggt ctctatgacg gcaagcactg tgagaagcac cgctatcttt
   142381 gccatagcaa ctatgttatc cagattaacg ggtctcgcaa gagactccct ctttgcaaac
   142441 tactttggta cttctgctca atatgatgct tatcttgtgg caataatgat ccccttcttc
   142501 ctcagaaaga tattcgcaga cggcgccttg accatggcat ttgttccggt tttcaacgaa
   142561 aagctgaaga tttcaagaga aagggccttt atctttgctt ctactgtcat tgtctttgta
   142621 gtgattgttg ctggatcgat ttccgccgga gggatggttt tttcagaggg ggtagcctca
   142681 gtatttgccg ggggtttcga taaggatgca ttggacctga catcgagatt aattcgaatc
   142741 tcctttccat tcatagcgct tgtttctcta tgggcagttt actgcggagt tctcaacagt
   142801 ctcgatgctt tcttcattgc ggcggtttct ccaatgttca taaatctctc aactatcgca
   142861 ggaattctgt tttcagaaag gttttcacca ccaattgtcg gaccgacaat aggtttcttg
   142921 gctggcgggg taatccagct ggttgttgta gctctggcag ccaaatcgaa gggttttgtc
   142981 ttcaaaccgg gatacagcaa aagcgatgta aaagagttcc ttattctttt ccttttttcg
   143041 gccgtatctc cggctataaa cgaaatcaat tcctttgtag atgtaagagt atccactgaa
   143101 ctgggtcgag gcgcagtctc tagcctgggg tatgctcaga ggctttatca acttccactg
   143161 ggcgtcttcg ccgttgctgt cgccactgta gcacttccaa ggttatcgaa actctccggt
   143221 tccgatgcgc gagaccgttt caggaaggct ctgtgggact cattgactgt gcttgccttt
   143281 cttatcatac cttcgactct gggtcttctg gttctgggtg aaggaatagt tcggattctc
   143341 tttgagaggg gttcttttac accatccgat accgcgttga ccacgtctct tctttacgga
   143401 tatactctgg gattgccttt ctatggatcg tatggagttt tatcaagggc ttactatgcg
   143461 agaaagagtc ccagaactcc gactatcatc tccgcaatca tggtaagcgt caatgtagct
   143521 ctagatattc tgcttggttt tacaattggg ccgcttggcg ttgcattggc tacaagtgcg
   143581 gccggaatcg tcggcacagt taccgtttcg gttgcactgt ttaggtggag cggatacgac
   143641 agagaaaaga ttattgctat tttgaagata atacttgctt cgcttgtcat gtcatgcgtc
   143701 atgattgttg ggaggacttt atttggaacc ggattcattt cgacttcgtt gactcttgtt
   143761 gcaggaataa tagtgtactt tcttttcgcg aggatacttc gtgttgggaa tctcttcaga
   143821 ttgagagatc tcgtgcggaa caaaagaagc agctctcaat aaatagtcaa cgcaacgggt
   143881 ttgtattcca tcttttcaca gtaaaagacc tccttgaatc caatgctctt cagcagaagt
   143941 actgcatcgg aaacgtgact acctacgtcc tcccttctat gagaatctga acctattgtc
   144001 acaaacttcc ctccacagag aaagtacttc tccacaatcc agcgttgcgg tgaaggttct
   144061 ttataaggat aacgccagcc cgatgtgttt atctcaattc cgataccggc tcttatgagc
   144121 ttcctcagta attcgcaaag aagttcctcg ttgtcaagag ctttatgacc gggaagatat
   144181 cttctgagaa aatcgatgtg accgaaaaaa cccggtctgt cgataccgga aatcgcctcg
   144241 attgcctctt ccagataaag cgtccaaaca tccccggaag tcaggtcttt aattccggga
   144301 attccgtgaa ccgagtaaat gaagtaatcg aagtcacctt caggcaaggt ctttgcgcat
   144361 gactgaagcc cgacttctag acccgccaga accttgacga ttgaattctc tttcatgcga
   144421 gaaaggctct tcaaatagat ttcaggatct ttcatcctgt agctatcagg gaaggaatcg
   144481 tcaagatcgt catggtcgga gattcctata actttcagtc ctttgagaat tgcttcctca
   144541 acgatttcct cgacaggtgt cttactgtca ggggaaaagg agctgtggtt atgcagatct
   144601 atagacactt ctcagtcaca aagaactttt agaagctcag ctcgcaaagt gctttcgatc
   144661 cttcctctaa aagcagccaa cgttaagggt agttttcctg ataccgtaac agtagaatcg
   144721 gcaaacttca cagaaccttc tgtcttcatt ccggccgcct tcaacctgaa tctcagggag
   144781 tcgtcttccc aggtcgagtc aagggcgaac ttcccttcat attcactctt cagctcttcg
   144841 attttcttat tcagtctctt tattgcctct tcaattccta gtgtgtgctc gatgataacg
   144901 gaaagttctt tcaaatgaat cacctctctc aattgtatag taaataagca gccggtagta
   144961 taagcggtct catggtaaaa ttttcacgga ggtttagaaa tgaaaaagat ctacttagtc
   145021 attgatacag aaacaactgg atccagtccc cttgaagggg ataggattct tgagattgca
   145081 gctattccgg tctatggaaa caagatcttg cacaatctat catttcagtc gctcgtgaat
   145141 ccgcttgtca tgattccagc gcagattaca ggcattcacg gattaaaaaa tgaggatgtt
   145201 ttagatcaac ctacaatggc tgaggtcttc ccaagattcc gaaactacgt tggaggcgcc
   145261 acgattgtag gtcataacat cgttaatgac atgacttttt ttgatattgc atcgaaagaa
   145321 accggaatac tgcccttagc aaataactac attgacacta ttgacatagc tcatgaagtc
   145381 ttttccgaag ggccttatgg tcttaagagc attgcgaaga ggttgagaat aagagatgtt
   145441 ccgactcaca gggcgatgga cgacgccaga gtaacagcaa aagtctttct ggcattggca
   145501 agaagactcg ggggcatatc tcaaatggtc aaatacgaaa agaagtggag gggttaagtt
   145561 tgataaaaga ctttatactc gataccaatg ttcttgttca tgatccatac tgttttgaca
   145621 attttgagga caacaatcta attattccat ttcccgttct tgaagagata gacaagctga
   145681 aaaaaaattc gggatctgtc ggccagaatg ctagaaaagt caacaggttt ctcgattcgt
   145741 tgagatctaa gggcagactg accgagggtg ttaggctgga gtcgggcggt acgctgagaa
   145801 tagcagtctt caatgaattc aagagccagc ttccgccttt tgcagcaagc gggtacaagg
   145861 ataacgctat ccttctttat atgaaagaac ttgaactcgt agataaactt cctgtgatac
   145921 ttgtcagcaa ggatatcaac atgcgagtca aagccgacat aatggggatg aaggctgaag
   145981 actaccttca cgataaagtt gaaatcgacg acaagctctc aggagttaac acgtttgacg
   146041 actcagccct tagagacaag ttcatagata gggacgagct ttcggtggat gaactctccg
   146101 attccgttgg ggcaaatgaa ttcgttgact tcggaaaggg aatattcgga agggtaactc
   146161 ccgatggcga aatgatagtt cctcttgaag tcaaaatgga tacgagcagc tgggggataa
   146221 agcccagaaa ttacgaacag atgatggcca tggaactgct tttggacgat agggtgaggg
   146281 tcgtctttat accgggaatt gccggaactg gtaagacgct gatttcgctt gcttgtggcc
   146341 tgaggaaggt tgtagatgaa aagaagtatg agagattgct tgttgcaagg cctattatcc
   146401 cgatgggaca ggatataggt tatcttcccg gctcgatgga ggagaagata gatccttgga
   146461 tgactccaat ttacgacaat ctctatttgt tgttcaacaa tcgccatatg gacatggact
   146521 cattcttgaa gaagagagaa caactggaag ttgaggtcct aagttatatt agggggaggt
   146581 caatacctaa ccagtacttc ataattgatg aggctcagaa cctttctccg catgaaatca
   146641 agacgattat aacgagggtg ggggagaata ccaagatcgt cgttatcggt gatccctacc
   146701 agatagataa ttcctatctc gatgcttaca gcaatggctt gacatatgcg gcgtcaagat
   146761 ttagggacaa agaactcgca ggccacatca ctctgatgaa aggtgagcga tcagaacttg
   146821 caagtctggc cgcagaatta ctatgaggtt tatgtatggg tgaggtattg gaatatgatc
   146881 tctttgtcag aatcaggcca gaggacattc atgtgctttg ctatattgcc gaggcagaag
   146941 acaatctgat gaacatcagg catgttactg acgaaggact acttaagata atcgtcccca
   147001 ccgatctgct ggaggaagtc aagagttttc ttcaaagcat taagaaccgt attgaactgg
   147061 aagtggtgga gattcgtgcg aatcctggac atacttgata acgcggtacc gaacgaaaag
   147121 gcagaaaacg taagagagat agactacgag aggctccgaa aactcggtta caatacgatt
   147181 ctcttcgatt atgacaacac aattgctgta tggagagaac ctttcgacat gagaaacaag
   147241 cctgttatag atagcttgat ttcatctgga atgaaggttg gggtcgttac taacgggcca
   147301 caatcgagag tcaagaacct gaaggatctc ttcggggaag acctgaaagt gtatcactcg
   147361 atgaggaaac ccgggacaaa ggagctgagg aaagttctca gcgagatgaa atcgagaccc
   147421 gaaaagacag tgataattgg tgatcttttc ttcacggata taattgcggg aaatagaatg
   147481 ggtatgtatt caattctggt atcccctttg gtggatatca gccataaatg gtataaaaga
   147541 cttctgggga aggtcacaat tgcggtctat ctcgtttttt tcttcactat tggttggata
   147601 ttcagaaccg gaagacttgc aacgccccac cttttcgcaa cgaccgttat ggatattgat
   147661 tttgattcac ttaaagattc tggttataga ctgataatat tcgattttga caacaccctt
   147721 gaaaagtggg gagctaccag cgtttcgaag gagaaacgat tgctgataag tagagtagag
   147781 cgccttggcc ttgaagtggt gctgatttca aacggaaaat caaatagact tggaaaaatc
   147841 gaaagagaac tccgtacgac aaaggttatt agtcgggcaa gaaaaccttt tacattcaaa
   147901 gcaaaacgtg tattgaaaga tttcggtata cctccataca agacggtaat tgttggtgat
   147961 cagctattca cagatatcat catggggaac ttattgggtg cttatacggt taaggtagaa
   148021 ccgatttccg acagggagtt cttctggacg aagcttgtga gaagagtaga gagtctcata
   148081 ttgagcaaga tgaggaagca tcccgaagtg gaggcgatgg atagatgaag tgcggtggtt
   148141 gcggtgttga gctccagcat gatgacccag ccggtttagg ttacatatcc agatcggtca
   148201 tggaatccag gcttcagtcg ggaaaacaag tactgtgtcg tagatgtttt ttgatcaaac
   148261 actatggttc tctttctgaa ggcgatagag tcgttcattc aatcgacaac ctgaaagaat
   148321 acctcgatct tgcgcaagat gttatctacg tcattgatat ttcagatttc gacgggacct
   148381 gtagaagaga tatcctgagg ctcctgaagg aacgcaccgt tcactacata ctgaacaaga
   148441 tagatcttct gcctaaagag gttaaaatcg acgagataag agactgggcg gccagtatgc
   148501 ttaaagtacc taaggaaact gtaagacccg tttctgtgct gaaaaattat ggccttaaat
   148561 cactcttctc ttatttgaaa tcggcttccg aggagtttgt ctccatagga gtgacaaatg
   148621 ttgggaaatc ctctgttctt aatggtctta tacaatctga agaacttaca gtgagccgat
   148681 tccctggaac gactgtgaag gtagcttcta agaaagtcgg cggctccgca ttcaccgttt
   148741 atgacacgcc cggaatcttc acaaaggaca ggataacaga ccttctgact gtagaagatc
   148801 aaagcagatt ccttccacgc aagaagcttt gcagatcgac gattcagttt cacgatgttc
   148861 gtacagtttt ccttggtgga tttgttaggt tagatgtaaa aaatgagagc gacccggtcg
   148921 ggatattcca tgtctttgtt cctgaaagtg taacagtgca cgagaccaat tccgatacgg
   148981 gaaacgaaga gtgggacaga tggtatggag gaatcttgaa acctcctttt tcggagtcga
   149041 ttcgaagcaa ttacaaatgg aaaagcgaaa gattcagact aagaaccggt caagagcttc
   149101 atatattcgg aatgggatgg ataaatgtgg caaaaggtcc tctgacgatc actgtaacta
   149161 ccccagaggg tgttgccaca aaagtaagaa agggacttat tggcgcgaaa aaattcaaga
   149221 agcaaaaata actggaggtg tttttgtgaa actcaagcaa ccggcaatca agacaggcgt
   149281 ttcaaaccgt catcttcatc ttagtgcagc agatatcgag agtcttttcg gcaagggtca
   149341 tgagcttact ccagtcaagg atcttggaca acctggccag tttgcatgtg atgaaaaagt
   149401 gattctcgtg ggtcccaaag gtgcgatcac aggagtaaga gttcttggac ctgccagaaa
   149461 ggcaactcag atagaggtat ctagaacgga ttcctttact cttggcataa agccaccaat
   149521 taaggattcc ggcgaccata cggatacgcc cggtctcacg attgtcggtc ccaaaggaac
   149581 ggtagtgctg aattctggag taatgctggc taagaggcac atacacatga cgcctgcgga
   149641 cgccgaaatc tttggtgttg aggacaagga tatcgttatg gtgtatgccg aaggtgccgg
   149701 aacgaggaaa gtagtattcg acgatgttct agttagagtt cattccagtt acgccctgga
   149761 gtttcatgta gatgtagacg aagcaaatgc ggccgttctc aacaacgacg atccggtata
   149821 cattgttgag gagctttaag tgatttatct ggggacgagc ggatactcat ttcctgactg
   149881 ggtgggtact gcttatccag cggagataag ctcttctcag atgctgaggt actatcattc
   149941 tgtttggaga ttcaactcag ttgaactgaa ttttacttat tatagaatgc caacatataa
   150001 gacagttact ggaatgatta gaaagatacc tggtgagatg atctttgcag taaaagcgcc
   150061 gggaagagtg actcatgaga tatggaagaa cggtgtcaac gatctacaag agatctcgga
   150121 tgagttctat aatgctcttt ttccgatgaa agaagaggga agattaggcc cgctgctttt
   150181 tcagttcccc tggtccttca agaacaacaa agagaatatg tcttacatta aagcagtcgc
   150241 aaagactttc agttcagata agaatttgct cgctttcgaa ttcaggcacg agagctgggc
   150301 agctgacgag gtcttcgaaa ccattgacag attgggtgtg gtacctgtaa ccgttgatga
   150361 accttcattt ggtggattgt ttccatatct tcccagatca ggttccaggg gtgcttattt
   150421 caggtttcac gggagaaatc ccgattggtt caacagcaag ggcagtgaga ggtacaacta
   150481 cgattaccct aaaactgatt tgagacgttt tgcactcgat gtgctagaat ttcataagca
   150541 tgatctaccg gtgtatgtat tctttaataa ttgctacatg ggaagagcag ttcacaacgc
   150601 tctcctgatg agagacatct taggaggtgc ctgaataaag aatgaccttt tctgattaca
   150661 taaatgtggt tctcagaatg atagctgagg gaaacgaaag tcacgagatc tatgaacagc
   150721 ttcagaaact tggcttgagg gaagttgaga tcaccgaggt ctataatgcc gtaattagca
   150781 tgattacttc cggttctgaa tcaacggaag tcatcctgga cggttattac gacccaagga
   150841 aaagagtctt ccttgatgaa gagctgaaag cgataattag cgttccaggt ctgtataaat
   150901 ctcttttgtg gggagatatt tcccctggtg attttgagag aacattaagt gatttttctt
   150961 cctacagttg aaaaacccgc cgttaggcgg gtttcctcta cgtaatccat tttgcaacag
   151021 gtatgaagta tctgtgaggc agtctttcgg ggttggggat tactctaaca gtgtatgcaa
   151081 gctgcccctc ttcagctacg gtgaatttgc ctccatagac atattcgccg tgaacatctt
   151141 ccttaactaa cttcatgggg taaagactaa acccgtccat ttctccttcg tcattcatct
   151201 ttgccacaaa gatctcgacc tgaacctcag aaggatccat agtaccgaga tatatgttcg
   151261 ctgaaacaga aatctcctgt cctaccttaa cggactttgc agaagtctca cctatcatat
   151321 tgggcttgat tcttatggca ttccagctcg aactgaattt gtctttccag atggcaacac
   151381 tctttgcgag tttgaaatca tctttttcga cttctttcga aagatcggcg actggaaaat
   151441 atagatcctc agcatagtct gcaatcatcc tgtgagtatt gaatttgctt ccgatttctt
   151501 tgatagagtt tttcattctc tttgcccact ctttggcaac tcccttttcg tctctagagt
   151561 agtaaagggg aattatctcc ttttcaagtg agctgtaaat ggaaacggaa tcaattctat
   151621 cctggagctc gtggtccttg tagtctctat tgtcgccaat ggcccagcca tttactccat
   151681 tgaagccttc tacccaccag ccgtctagaa cactgaaatt cagaactcca ttcataccag
   151741 ccttctcacc actggttcca ctagcttcat gaggtcttct tggcgtattg agccatatat
   151801 cgacaccgct caccaagtgt cgagctatgt tcatgtcgta attttcgact ataatgattc
   151861 gacttttaaa ttcaggagtc ctggaaattt cataaagctt tcttatcagt tcctttccag
   151921 ggtcgtctgc agggtgagcc ttcccggcaa agacaaactg aaccggtctt tcagagttcg
   151981 ttattatcga cttcagcctg ttgatatcct tgaaaatcaa cgtagccctc ttatatgtgg
   152041 caaatctcct tgcgaatcca atcgtaagaa catcctcggg aagagcttct tcgacttcca
   152101 taagatcttc tatggtttct cccagtctca ttctctgcct cttgagcctg ccttgaagaa
   152161 agtttctggc ccttgatttt agcgacatgt gtaattccca gatctcctct tcgggtattt
   152221 tgtcaactgc ctcccagact tcggggtcat aaactttttc gtgccaaccg acaggcagat
   152281 atttgtcaag aactttgact agatttgggt tcagccaggt ccaaatatgt actccatttg
   152341 taacatgaga tataggaacc tcgacagccg gtaggtcggg gtagatatga ttccaaaggt
   152401 ttctgcttac ccttccgtga agtttgctga ccccattagc gagggcggac agtctaaggg
   152461 caagaacggt catgctgtat tgctggcctc cgcccgggag tttttcaatc ccaagattta
   152521 tgaattcgcc tctctcggct cttattgtgc tccagaaatc acggaagtat ttgtcgatta
   152581 tctcgaggct gaacgtgtcg tttcctgcag gtacaggagt gtgggtggta aagacgtttc
   152641 ctgatttgac gatcattgaa gctgtggaca aatctatcga gtgtttctga accaactctc
   152701 tgattctctc gagagaaagg aaggccgcgt gcccttcatt catgtgccaa acattcggcg
   152761 aataattgag cattctgaga gcccttactc cgccgatgcc aaggagaatc tcttgtttta
   152821 tcctagtttc ccggtcgccg ccatagagtg tagccgttat gtttctgtct tccggctcgt
   152881 tttggggaat gtctgtatca agtagatata gcgatgttct tcctacggct gccttccaga
   152941 ttttcgccca gacttttctg cctggaaaat cgatgttaac aaatatctca tctcccgagt
   153001 catcctgtgc gggaactacg gggaagtctt caaattcata agagtggtat atattctgtt
   153061 gccagcccga ctcgtcgatt ctctgttcga aatagccgtt cctgtataaa agaccgacgg
   153121 caacgaagtt cagtcccata tcactggcag tcttcaagtg atctccagca agaatcccaa
   153181 gacccccgga gtagatcggg aaagattcat gaaatccata ttcggcacag aaatacgcga
   153241 tacattgttc ctttctgtcc ggatgttctc tatccatcca ggtatctttc ccacccatat
   153301 atcggtcaaa ctctcctaca acactttcat acatctcaat aaatggtttg tcttcggaaa
   153361 gctcataaag tctggtttgt cttatccttc gaagaagcct tacgggactc cttctttcct
   153421 gataccacat ttctggatcc atgtatttga acatctgttg agcgcggtag ttccaactcc
   153481 accaaaggtt ctttgaaagt tcttcaagtc ttattagctt ctccggtaat cgtggtaccg
   153541 ccatcactct ctcgaggaag tacatagcaa taacctccta attctggcaa ttctgtccaa
   153601 ttataccttt ttatacaagt ctatgctttc ctactatcgt ttagctcgtt atttttacga
   153661 attgctagca gagctacaaa aaagggagcc tagctccctt aattctatct aactttcgct
   153721 cttccattat ggtctttttc ggatacctct tttcttgtcg agtcttctct tatatgcaga
   153781 aatcttctcg ccgctatctc tcatgaactt ggctagtttc ttctcgaaat cctgacttgt
   153841 aggcgcctct tcagaacctt tgccttttag atcctttata gacagctccc actttccatc
   153901 tttcgtcgtt ccgagaacct ttgcttccac cttctgacca actttgagat agtcttcaat
   153961 gttcttcacg tactcccttg cgacttttga aatgtggatg aatccctctt ctccgccttc
   154021 aagatttacg tacgctccaa atttcttaac agaagtcaca tttccctcaa caacggtacc
   154081 cactttcaca ctcacatgac taacctccaa ttgactatat acttgagttt gcgcaaaacg
   154141 cttgctcagt aattgtctcc gtatttgttt ctgaacttct gtactcttcc ctcagtgtcg
   154201 agaatcatag aagacgcatc tccacggaag aagggatggc agctcgagca taggtcgatc
   154261 ttgatgtttt cctttgttga gtagaacttg tgctccgcgc cacacgtaca ctttacagtc
   154321 accagcttca tctcagggtg catttctttt ttcatcgggg cttcacctct tcatgttgta
   154381 atttgcacag aattcattat atcatagaga ggaaagcgtg tcaaagactt aattatggct
   154441 gaatgctgta ctggaacggt gagtaatgat gattgacgat atattgaatt ttgtgaaaga
   154501 ggctgcttct tccgaaggat tttacgcctt ttatagtaac agaaaggagc ttgtcgagag
   154561 gttaaatagg gctcttaaga gaagccctgt gagtagtgcg actgtaggaa agcttcgcaa
   154621 gagaatagcc ggattggccc atctctctta tgaagagatc gaattttcga tcgacatact
   154681 ccgcgaaaga gacagggcac cagaagaaag ggttgaatat gcttctacat tgttcaaagt
   154741 gtcagtctca gatatggggc acctgctttt ttttgtggat ccgagaaaca atcctccagt
   154801 tactggcccg gtaaaagaga gaattcaatc gatcgatgat tacaaagaat ggcaatccct
   154861 aactcgctca gttggtgagc atgggattca ggatttnnnn nnnnnnnnnn nnnnnnnnnn
   154921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   154981 nnnnnnnnnn nnnnnnacaa agaatggcaa tccctaactc gctcagttgg tgagcatggg
   155041 attcaggatt ttgttatgct cgaagctgct ttgcttttcg aaagggagga gaataataga
   155101 agctccaatc tctctgaaag actaataaga gctgtttaca cagatatctc agaaataaag
   155161 acccttagaa atgctgtaac ttctctttca aaatccgaaa aaaggctgct tagcaatctc
   155221 aggttcacac atccctacgt taagacggcc atcatgtcga atcattctaa acctgttgtt
   155281 attgacggca gcaatattgt tttttcgatg accgaacatg ccgatctaaa aagaatcgat
   155341 gatctcttcc taagaatggc tctttgcagg attgctttgt tcccttatag gatagtgttt
   155401 gatgcaaata ttagatatac tatcggtggc tttcagcagg agagtctcaa caggcttctg
   155461 tcacttcctc aagtcgagac atactctcct gccgatgatc ggctaatctt tttggcgaga
   155521 gagggcggtt cggcagtgat aacctacgat aggttcctgg accatgctgc tgaagatatt
   155581 acgattatca gaccggagga aatcgatgaa agtctcagac tatgattttg agcttccgga
   155641 agaactgata gctcagaaac cagccattcc aagagacagt tcccggctat tggttattga
   155701 tagggagaca tactcattcg agcacagagt gttcgcagaa atcgagaatt ttcttcatcc
   155761 gggagatgtt cttgtcttaa acaacagtcg tgttattccg gcaagactcc tcggtaaaaa
   155821 ggaaacaggg gccaaagtgg aggtcgtcct tacggaaaag cttgacgggg gcctctggaa
   155881 agccatagtc aggcccgggt caaaaatcaa acttggaaac gtgctcttgt tcaataagat
   155941 ttcctgcaaa gtaatagaac actgcgatga ttcaagcagg atactagatt ttggtagcgt
   156001 gacagatgaa gacgtaaaaa aagcaggtct cttggcaatt ccaccttacg tcaaatacta
   156061 cccggacgat ccagagtcgt accagacggt ctattctcgg ctggaaggct ccgtagctgc
   156121 cccaactgcg ggattgcact ttacagatac tcttttggaa agacttagga agaaagacgt
   156181 tgatgttcaa ttcattactc ttcatgtcgg aattggcaca ttcagacccg tcaaatcaga
   156241 aacaatagac ggccataaga tgcacaatga attcttctcc gtttcttcgg aggttgcaga
   156301 gagattggag cgagccagaa aggaaaagag aagaatagta gcggttggaa ctactaccgt
   156361 acgaaccttg gagagcataa ttgctcgcca cggttccatc gtaggtgata gcggtcatac
   156421 ggatattttt atctatcctc cttatacatt caaagcgatc gactctctca taactaactt
   156481 tcacttaccg agatctaccc ttcttatgct cgtttctgca ttcgccggga gggaactgat
   156541 tctctcggcc tatagagagg ccattataaa gaaatacagg ttcttctcgt tcggtgacgc
   156601 ctgcctgata ctatagagaa gcggctctga gatgctagaa gttcccatgc tggagcctta
   156661 aacaaaaaag gtgtcttctt tgcgggaaaa aaggtgccct actgctcttc ttcgagcatt
   156721 ggagagaaaa aatgcatcca tgtcatcttt aggattattg caaatggcac ggcaataatt
   156781 gccccaaaaa gcccccagag ctgcccggcg ataacaatca tcaagataat tatcaccggg
   156841 tttatcctgt tgtatccctt catgaatacc aggaagagta taaacgcaga tgcatgcaca
   156901 actactagaa ttatcattaa gacaagcaca agaactagat cttgagtaga cactgccagc
   156961 aagaaaagag ggaagaattc aagagggact ccaacaatgg gtattagatt tgtgaaggcg
   157021 gcccacatac ccatcaaaag actgtactta agtcccatag aatgcacaac aatgccaacc
   157081 accaccccaa cgatcatggc gattactacc actgaaatta catatcgctc tagattcttg
   157141 taagtatctt ccaggaaact catagctatt ccacgcactt tcttggggta gaatctggga
   157201 ataaaatcct tcacagtgat gaagtagtaa ctcaggtaaa tcgcgcctac tatggtaaag
   157261 aatagaacaa gtcccgtgct ggttaagaag cttggaatct tttcaagtat accaggcaga
   157321 aatttcatta ggctgctctg cacttgaatg aggaagtttc tcacactttc gtaggtggct
   157381 ctttgtgttg atgggtcgac atttttgggc atgaagtaat caactacgtt gcgccacagt
   157441 tcttgtgaat ggactaccat tgcattatcc agaaccgaag ctgaaagcag ttccgccgct
   157501 tccgggttaa gataattcga aatgattttg tgtatctctt catatgaagc gtttttttgc
   157561 tcaccgctgc tgataattac gacataatcg cttctgtttt tcagggtctc ctcaagtaag
   157621 gcaagttcct cattcaggtc ttccgagttt gcggtgatcg ggtccatgat ttctgagtag
   157681 taacgtttga gatcttttga cagcagatcc aaggattcaa cctcttctgg agagagtttt
   157741 gaaagaaact cctcttcgga aagaaatcta tctatgtttg catttacttc gaaagaagtt
   157801 atttcggtct cttcagcgga gggaaagaag tctctcacta tgttatagaa tttggaagct
   157861 tcttttgcga cgggggtagt caggaaaacg atcagaagcg tgagacccga aaaaatcacc
   157921 gccagcccta tgccaagaga gatcgacctc ttaatgtgaa aacttctttc tgcaaatctc
   157981 gcccatggct ccaatattat gacaaccgtg aaggttgcga agagaatcat tgccgtttgc
   158041 aacgaaactg agaataccat aaaaaagaga gccaggtaga acagggtgaa aattgcagca
   158101 ttgaaacgag gtttttttaa aatctcacga ctcactttat gcccccttca aagggagttt
   158161 aaagagtctt tcaatctccg gaatctcctt tgtaatcccc ataagcacta tgagttttat
   158221 tcttgcttta atcggggata gttcctgagc gaagattgca ccaatatcgg ccagactctt
   158281 tcctccacca acatatccgt aaactccaag aacacgacct ttgaagcacc ttgaagtaat
   158341 gattaccgga acccctttct cctctattag gtattttact acgtgataga cttctacagg
   158401 tacatttccc cttccaaatg tttcaagaac tacccctttg aatccaagat cgggaagtat
   158461 ccgcaggatc gaaccgtcgt ctcctgtaaa ggtcttcaca agggctattc tcgattcgat
   158521 cttttctaca ttgaacactg ccctcaacaa aggctttcta aagaagatca ccgtgtcttc
   158581 atccacgatt cccagtggtc catgaccggg agagtcaaaa gtagcaacat tgctagtgta
   158641 tgtctttgtg acctctctgg cggcgtggat ttcatcgttc aggcacacca ttgtccccat
   158701 tccaatcgca aggggactaa ctgcaactct aactgaggac aatacatttc tcggaccatc
   158761 ggttcccatt tcgtcgatgt ttctcattgc agcagtacag ataactggtt tctcgcttct
   158821 gactgtcaga tctagaaaat acgcagtttc ctctagcgtg tcagtgccgt gcgtgataac
   158881 tactccacaa acttcctctc tatcgagaag cttctgaact accttggaaa gcgcccacat
   158941 cgtctcggga tttatgtatg ggcttggaat gtttgaaaac tcgtagtgct cggtacaggc
   159001 cacatcttta agtccgtcga cgtccgccac gtgtttgttc aatccctgag ccggtacaga
   159061 agcaccagtc gcaggatccg taatcattgc gatcgtacca cctgttgtga ctataacgac
   159121 cctctgtttc atagccactt tcctcctagt tttgtcttta ttaatgacag tgtcttgtga
   159181 gtctctggac caacaatctt gattagctct tctcttgttc ttggtctctt tttcataagg
   159241 gactttgccc tgtcccttcc aattccgggg atgaacctca tttctctttc acctaggtcg
   159301 ttgagttctg ttccaactgg aagaccggta atggatctca ttccatggtc tattactaca
   159361 aaatcggtta tttcttcaaa gcccacgggg ctaccgacta gaataggata tgtaccgagc
   159421 ggtctgccga agtagatcat cccctcgcta aattcaggga ttacaccacg gatgatcgct
   159481 cccagtggga aggtcttctt aagcatttta gagtctattt cctttcttat acggtgtttg
   159541 tgtttctcga atagtctgtg gtcgattctt ggtctagtca tattgaatat tcgggcgcca
   159601 ggaaagatca ttgtctgtct gatgttgatt cttctcaaaa gaagacccct ttcttgaatg
   159661 ctgacaagct catcgtagtc aatgtcgaaa ctctttctac tctgtcctgg aaggccgtaa
   159721 agtaaattga ttcccggcaa gagcttcggc actccttcaa ctctttcttt gccgatttcg
   159781 ttcaccaact cgattgaaaa tattatgtct tgtacgctgc caccaagatt gttcaaagaa
   159841 cggacttcgt cgtcgaagct ctcaaccccg aatgatatgg tgtccccggc tgtattatac
   159901 ctaactatgg attctatgac cgatcttgat tctgacggat gatgagcaat aaagattgga
   159961 tttccattat caatatgaaa gacttcaggc tgaatgatac tccttgctga tttgaagagt
   160021 tcttcactca aggatggatc aagagtgtct cgtctccggt catacccata tgcaattaca
   160081 tttgcactcc ttccgaatct aattgcctta actccaactt ccttaagttt ctcgaattca
   160141 gcgacaattc cttttatact tctccattca aaggagccat agaaaatgga ttcagtacaa
   160201 aacgagcaga atccatccaa cctctcgcaa ccagaggaaa tatcgatttc aaccatcaga
   160261 tccgggaaac aaggatgaag cctgattaca tcggcaccgg ccggataaag tctgttaagt
   160321 agagagtaac gagaccctcc cgagtctaga cccaaaaagt ccgcaatttc aaactcatcc
   160381 ctcaagagat aatctgcttc tccaaaatca ggagtgaagg ccgtggtccc acctctcaaa
   160441 gtgtatccga cagataccac cgggccgctg acgattttcg tgggatcgga atttgatttg
   160501 agcagaagag atatttcgga acttgaaatc ggagttcctc caatgtatct tccggaagta
   160561 gtgacacccc caaatacgat caggaagtca taatcgttgc ctttctccca cagtccttct
   160621 tgccttaccg agtcgatagt gagataatct gtttcgatac ctttcaacaa aaaggctcct
   160681 gcaatagcgc gaatccttgg tgaaatgtac ggtggcacac cgaaggctgc cggctcatcg
   160741 gtgtatccgt ctatgataag agcattcatc ggttacctcc acgtgcgagc tataccattc
   160801 cagtgtataa taaaaacagc gattaatggg agggaaagtt ttgccttttg aaagacattg
   160861 ctcattttgt ggaagatctg ctgcacaggt aaagaagctt atctcaggaa cagaagggta
   160921 catttgcaat gaatgtgtcg atctgtttca tgacattatt cagcaggacc agaaagccaa
   160981 gaaggagcct ggtaaaaagc tgcctacccc agcagatatc aagaaagaac ttgataagta
   161041 tgtgattggt caggagaacg caaagaaaac gatctctgtc tccgtgtaca atcactacaa
   161101 gagagttttc aatgatgtag atgacgttga aattgagaaa tccaatataa tgctagttgg
   161161 gcctaccggt tcgggaaaga ctctcattgc tagagtgctg gcccgtattc tggatgttcc
   161221 gtttgcaatc gcagacgcta cccctcttac ggaagccgga tacgttggtg aggatgtaga
   161281 gaatattgtt ctaagacttc ttcagtctgc gaattacgat gttgagaaag ctcagctagg
   161341 aatcatttat atcgatgaga tagataagat agcaaggaaa tctccgaatc catcgatcac
   161401 gagagacgta tcgggagaag gggttcagca agcccttcta aagattgtcg agggaactac
   161461 tgcaaatgtg ccgcctcaag gaggcaggaa acatccctat caggaattca taaaggtaga
   161521 tacaagcaac attttgttca ttgtgggggg cgcttttgat ggccttgaca gcgtaataaa
   161581 acagagagtt cttgattcct caatgggttt tggagcaaag gtaaagggca agaatcagct
   161641 taggcttgga gaagtccttc agcaagtagt accggatgat ctgattcagt acggcttgat
   161701 tccggagttt gttggcagat tcccggtttt ggcggctctg aatgatctcg atgtggagga
   161761 tctgaagagg attatgagtg agccgagaaa tgcgattctc aagcaatacc agaggcttct
   161821 gggactagac ggtgtagaac ttgagttttc ggatgatgcc atgaccgcga tcgcagaaag
   161881 ggctatgaaa agagggacag gcgcaagagc gctgaaaagc gtaattgagc aggtcatgct
   161941 tgacataatg ttcgatattc ctaccatgaa taatgttgag aaggttgtta tcaacgagga
   162001 tgtgataagt aaaaactctt ctcccgaaat aatctctaga gaatctgcct gatgaaagtt
   162061 ctggctctag agagctcgtg tgatgaaaca gcggtggcca tcatcgaaga tggcctcctt
   162121 ttatcttcac agatttcttc gcaaatagat atccacaaga gatttggtgg ggtggttcca
   162181 gaaatagctg ccagaaagca tctggaaatc atagatagaa tggtttcccg ttcgctcgaa
   162241 gaagccgatc tggatatcaa agagatagat gtatttgctt caactatggg tcccggactt
   162301 gtgggatcat tgctgattgg tctttctttt gctaaaggaa tggcgatcgc gatgaataaa
   162361 ccatttgtcg gtgttaacca cttattcggt cacgtctatg caaatttcct taaatacccg
   162421 cttctcaaac ctccctttat ggttcttctc gtttctggtg ggcataccga gatcctagtt
   162481 cttaaagatt tggataggat cgaacttgtg gggaagactc gcgacgacgc cgcaggagaa
   162541 gcatttgaca aaattgcaag gctgcttgga cttgggtatc ctggagggcc ggcaattcaa
   162601 gaggccgctg agaaaggaca ggctaagtat ccttttccta ggccacttcg tgaaaagggg
   162661 aatttcgatt tttccttcag tggtctcaag acttcggtgc tgtacttcat gaagaaaaat
   162721 cctctggctt cagtttatga tgttgccgcg tctgctcagg aagcaattgt cgactctctc
   162781 gttaccaaag tattcgaagc agctgaaagt ttacatctac atgaaatagc ctttgcggga
   162841 ggggtcgctt cgaactcact tcttcgagaa cgggcaagag tgcaatctga agttagcggg
   162901 atcaaggctt acttcccacc ggtagacctg tgcacagaca atgctgcaat gattgcatta
   162961 gtggcatatg aaaaagctaa gagggggcta ttctcccctc tttcaacaaa tgctgtacct
   163021 tacctgactt tggatttctt ctaactattc agacttgctg gtagaagttg aaggtttgct
   163081 actactttta gaagctgatg gcttcttcga atcggtactg tagtaacccc cgcctttaaa
   163141 aacaactccc acattattaa ccagtttctt gagatggccc ccgcatgctg ggcagatcgt
   163201 aaggggttca tcggacatct tctggaaggc ttcgatttct tctccacaat cctcgcactg
   163261 atacctgtaa aacggcacgt gagtccctcc ttagcatcga atttttctat tagctgtatc
   163321 tattctattc ctcatcggcc gaagatggaa tcacacagag aaattccaga tctccgtttc
   163381 ccgtgttcct gaactggtgc tttacgccgg agggaacaaa aacatagctc cccttaccag
   163441 cattaatcac gccctggtca gtgacaatgg ctgcttcccc ggagatgata aagacttcat
   163501 gttcccatgg atggctgtgg tgaggactat atcctccttc tccgacagta aacagtctca
   163561 tcacaaaatt cgtagcgccc ttcttttttc caataaggac acgcttagta acgctcttta
   163621 cattttcgtt gtcgaaaaac tgaggctcga catcaagcgc tttgcctacc agaattcgtt
   163681 ctgacatttt catagcgcct cctttttgaa tcaaacgctt tatcgcatga gtattttctg
   163741 tctctgtatt cgaccacaac ttttctgtaa ggttcgagtc ctactgaata gcttttcaga
   163801 tcttcatact tggaaattac ttcgtgaacg agccttcgct caaaggcgaa catcggttcc
   163861 atcacgatct tgcttctcct ggatattgct gactttgccg ttctatgtgc tatctcttcc
   163921 accagtttct ttctttcaag tctgtaatca ccgatatcaa cggttacgtt gactttggta
   163981 ggcgccatcc tgtttgcgta aatgtttaat atgtgctgta aagcggcaac agttttacca
   164041 tgcttcccga taagtctggc aacctcttca ccttcgatcc tgactctata cgttcttcca
   164101 acaagcttta cttcggaaaa ggtctcttca tcgaaattct tcagtatacc cttgaggaag
   164161 tcgtgaagct ttctttccca gtgtttgtcc aacaaggaaa cttcaattac cgactccctg
   164221 gatccaaata tccctaggaa gcccttggag cctttctctt tcactgtgac ttgcatttca
   164281 tcgtcataag caccaagatc tgcttgagct agcctgatgg cttcttcaac ggtctcggct
   164341 ctgtattcaa ctggattcat tttgtttaac acctcctgag catgtctaaa tctacttcac
   164401 gggtttttcc tgtaaaccta gcatctgtct taaagtcagc ccctttatgc cgtggcgctt
   164461 gtacactacc cagcttataa agaactgaag gacactctgg gttgtccagt aaagcagcaa
   164521 tccagaaggg aatgtggcga aaaggatcgg aaagattact tgcatgatta ctcccgacct
   164581 cgcagatctt gcgtcctgag ctgtgagcaa tgaactgaag attcctacaa ctaccgtgat
   164641 tattatgaac gggatgttca gtacgaagtt acctactgaa agatccttcc aaataaggaa
   164701 accaggcgta tatgccatta cttcgctgaa gtatcgaatg gcattgtata gaagtatcag
   164761 tactggcagc tgaaggagta ttggaaggca tccggaggcg ggattgtatc ccttctcctt
   164821 atacagagcc atcattgcct cctgttgctt tctggggtcc ttgtactttt tcttgatctt
   164881 ctctacttcg ggttgaagca tcctcatcat tagcattgac tttgtttgaa tgtgatataa
   164941 ggggtagagg atgaatcggg taacaattgt gaagagaatc aatgcccagc cgaagtttcc
   165001 agaccactga tatagccaga acaggaagta aactattccg tgatagatcc aggaaaagaa
   165061 ggtaactcct ccaaagtctt gcaagtcctt tacaatagca tcataatgct ccggaagagc
   165121 ttgtctcacg tgaatcaacc tcatagggcc cgcaaaagat tcaagaatgc ccctaccggt
   165181 ttcagattcg tatctctgga atttgaatgt tccttgcatt gaagtaattg cgaagagcgt
   165241 cttctggccg ctgaacttat catatgtcaa ataatatgag gagtcgggtc ctatcgtctg
   165301 attctggtaa gaaataaaag gcagagcgac ctcaatcttc tgattgaaat tcagttcaac
   165361 tctgaaatca tagtaaggac tattcaggat aataaacgac ttgctacctt gttcatagtt
   165421 gaagctaact attacattgc cttcttcatc tttgtcgtat gaaaaggagg taggcagaag
   165481 ctcggtaccc tctacgtcaa aaacgttgaa tccatctttt ccaaggcttt cgttgtacac
   165541 gtaaaactca accgtcttat tatcggcgta acttgttact cctaatacca aacctttttc
   165601 ggtgtccaga gttaccttta gagttttacc ggaaactatg agcgtattgg taccttcctg
   165661 agtggtaagt tccttgcccg ataccgagag cgtgaataat gtcacgaaga tcaaagtcag
   165721 aattatcgct cttttcattt ttcgtgcctc ctcaatagcg tgaaatgatc tggtacttcg
   165781 tcgaaccccc ctggattgaa gggattgcat ctgattattc tccagatgcc tagaattagc
   165841 cctttcagag ctccgaatct ctctattgcc tcatatgtgt atgttgaaca agtgggagaa
   165901 aacctgcatc ttgggcgggt tctaggggag atcttttttc tgtagaaatc tatcagaaac
   165961 agtagtatct ttttcatcgg acgtttctcc aattttctca gcgattcgtt tagtaatctg
   166021 tacaatatca taatatccaa cttttctctt cttaaacagg tcggatagat cctttcttgc
   166081 aatgaaaagt atatcatacc cttcgggaaa gacttgttta ttagttcggt aaacctcttt
   166141 aaccaatctt ctgaggcggt ttctcaggtg agccttcccg aatttccgtt ttatggatac
   166201 tcctatacga gaaaaaggca gaccattctt aacatacaat acaacaaaaa aagggtcaac
   166261 aattgatttt ccctttttga agactctgtc gaagtctgcc tttcgtctta gcctttccgt
   166321 tttactaagg gactgatctc tgaggttcac acagaaagtc tctttcttcc ctttcttctt
   166381 ctacttgcca gcactcttcg gcctccgact gtgcgggatc gcacaagaaa gccgtgtgtt
   166441 ctgtttcttt tgactcttga tggttggtat gttcttttca tgattaataa cctccaataa
   166501 atgcatcaca gtgaacatta tacggagtgc aaccttcact gtcaaggaca cagagggatg
   166561 cggagttaag tccaagagtc cacgaaagaa tttcttatgc agctgctgat gaatagtata
   166621 attgattgag agactggata gatgaaagct acctttttgc tgttgatgga gggagatatg
   166681 ctgctttcgc tggcggggaa agactttctg acttttaagg atttctatgt tgagttttca
   166741 ggcggtatga atgccataac cggtgaatcg ggggctggaa agactgtctt ccttaaggct
   166801 ctatgggcgg tgctgggctt ccccccgcta tgggataatg acggctcggg cagtgttgaa
   166861 ggtaactttg cggttgatga ttcgttgctt tctaaactgg gagacatggg tgtagacatc
   166921 aatggtgatc agcttcttgt aagtgtaagc ttcacaggac agcgaaccat atacaggata
   166981 aatggaaaaa tggtacccaa acaaattgtt cagtcagctt ttcgtgacag agtcgagatt
   167041 cattctcagc atagtagtat aagcctgctg gacgaatcca aacaccatat aattctggat
   167101 cacgcgcttg gaggagacac aagccttacc cgttattcag agctgtatgc agagtacatc
   167161 aaagtaagac gcgaactgga ttcctttatt gtagatcctt ctcagattga aagggaaaaa
   167221 aactttctgg agttccagat cagggagatt gaacaggcag agcttcaacc tcatgaagac
   167281 gaagtcattg aaatgaaata tacgagatac agaaacgccc agatgctact tgagacattt
   167341 caagaactca ctgatcttct taaggatggc gaactatcta tctataattc tctcaatgac
   167401 gcgcgagcaa ctgtcgaaaa aataaggaac tttggttaca aggactggct ggataacctt
   167461 cagattgctc tagaagaact cgattccctt tacactaaga ttgatgaaga gagaagcact
   167521 cttgagatcg atgatgagga attcagtgca attgaaagtc gtattaccat tatccagggt
   167581 ctgaagagga aatacggaga ctctattgaa aagatacttg tgagactaga ggagttcaag
   167641 aaagagcttg gaactcttaa ggagctggaa ggtagaaagg aacgcttgaa gaaaaacgaa
   167701 gaagagcttc tctcgtcttt gaaggaaacg ggaagaatcc ttgatcagaa aaggttggtt
   167761 agagcaaaag agatcgagga gcagatcagg gtacaccttg aggatctaag aatgaaggga
   167821 gcggagttga gatttcatct aaagcctgaa ttaatgccca gaagctacgg gacatccaaa
   167881 gtcacgatgg ttgttaagac aaatcccggt atggatttaa tggagattgg tagtgttgcg
   167941 tcgggaggtg agctttccag gtttttattg gctcttgaat ccgccttgaa gaatcagctc
   168001 gatctcaaaa caatagtctt tgatgaagta gactctggag ttggtcaacg acttggaaca
   168061 gtagtgtcag ggaaactgga ggaaatctca agggagattc agactattgt aataactcat
   168121 cttccgcaaa tcgcaatgag ttcagatcgg cattttgttg ttagaaaaca gcatgttcat
   168181 aacgaaacga tttccattat cgaggagctt catggtagtt ccaaggaacg ggaaatcgaa
   168241 gaaatgagtg gtcaaattcc agattaggag tatgagtaat ggaaagagaa gaattgataa
   168301 acaggattgt cgaagaaaaa ggagtggagg ccgctccagt gcttctcagt ctgctggtta
   168361 gcgaagacag tgagacagcg caaatatgtt ttgatgcttt aattcaaatg ggtaaggacg
   168421 tggttccttt tcttatcgaa aagatgagat cagaggatat cgaccccgta tcaaggcttt
   168481 atctagcaga tttggcaggg gagatcggtg atataagagc cgtgacacat ctttacgagc
   168541 ttctgagcaa gtacagtgat gaaagatcac agatagtaat ctacgaagct cttgctcgat
   168601 tgggggaagg cgaaaaagtg gtagatgttt tggccctgat gcttgaggaa aactatgacg
   168661 ctgaactgca agatcaacta atcatggcac tgagcaacac cgattcttcg gttgccgtta
   168721 aggctcttgc aggattttat ggaaacaagt tggccgataa atctacaaaa gcctttgcgc
   168781 tggagggaat ccattcgata cttgccagaa gacatgagct gaaaaactat ctcctgagcc
   168841 ttcataatgg taaggaaatt gctgaaaggc tttatcaatg gcagaaagaa atctaactga
   168901 ttactttaat tcaatctatt tggctggagc ggatgtaagg ctcgaagagc cgcttgaatg
   168961 gcacacgaca ataaggattg gtggtccagc cagagtattc gtttcaccgt attctgtaga
   169021 gtctctgagc gagatcctat ccttgttgaa gtcggaaaaa cttccgtaca aaatcatcgg
   169081 aggcggttca aatgtgatct gccctgataa atatgagggt gttgttgttt cgactaggaa
   169141 tctgaactta ctcagaagcg atggggtcaa ggtttttgct caggcgggaa cgagtatcaa
   169201 ctctgtcatc tggcattgtt tggcagaagg gttaacgggc attgagttct tgacgggact
   169261 cccaggttca gttggtggtg ccgtattgat gaacgcagga gcctttgggg gggaaatcgg
   169321 cgatcgggtg actagagtct cttatcttga cgggaggggt aaagtaatag acgttgactc
   169381 aaagagcgcc gggttttcgt atagaaacag catattcaaa gcagcagatg gaatcgttgt
   169441 cggcgctgaa ttcttgctga agaggggtga aagatccgag atcgaaacta agatgaaaga
   169501 aatccttgca aagagaattc agaagcaacc actggaacat cctagtgccg gcagtgtctt
   169561 cgtgagacct aaaccggatt tctacgttgg ttcaactata gaacgcttgg gattgaagtc
   169621 cttaagagtg ggaggagctg aagtttcgga aaaacatgca ggattcattg tgaatagagg
   169681 aggcgcctgt cagaaggaca ttctgactct ggtggagata attagaaaga aagttgaagg
   169741 ggccacaggc gttattctca gtactgagat agaaatttgg aaggaggttg agtaatggtt
   169801 gatgtaagaa tcgaagaacc tgataataat tttaccggga acggaggacc aaagaaaagc
   169861 tttttctggt ccggcctatt tgtgctgctt gtagtcgccg caatcgtagt agtctacttt
   169921 ttgagcggtt tctttcttgt gggtcccgat caagtggggt tgataaagcg gttcggcaag
   169981 tttactaaga gcgtaggacc cggacttggg tatcacctgc cctttcccat tgaaagtgta
   170041 gtcgttatcg acacttcgaa tttgagaaaa gaggagatcg ggttcaggac tatcagaact
   170101 gggacctatc agacctatgc aaacgagtcc ctaatgctta ccggagatgg aaatatcgtc
   170161 tcggtcgagc tggttgtaca gtactatgtg ggtgatcctg cgaagttggc tttcactatt
   170221 gttgacgacg gagatatcgt aagattcacg accgaatcgg ttctcaggga ggaagttgct
   170281 tcgagcacca ttgactcgat attgacaaca gagagggaca ctatttctat aaggactgca
   170341 gagagggttc aggaagaact tgatagactt gatacaggaa ttattgtgaa aaatgttttc
   170401 cttcaggagg tcgctccgcc acagcaggtt atcacagcct tcgacgatgt gaatagtgcc
   170461 aagcaggaca aggaaaagct aatctacgag gctgagaaat acactaacga cattattcca
   170521 aaggccgaag gagaagctgc ccagataatt aaggacgccg aaggctatgc tcaggagaga
   170581 atactaaatg ccgaaggaga ggccgaaaga ttcctcgaaa ttctcgagga atatgaaaag
   170641 gctccagacg taacaagaac gagaatgtat cttgagaccc ttaacaaggt tctttcggaa
   170701 gcaagcaaaa cagttgtgct cgatcagagc agtgttctga aactccttga tcttgaaggg
   170761 agcgtgaggt gatgaaatac aaagtcataa tttccgttgc gatcatcgtg gtaattgcga
   170821 tcatacttct gccgagtttc ttcttcatca tcgatgaaac tgaacaggcc gttgttttga
   170881 gatttggcga gattcagaaa tccatcaccg aggccggcct ttatactaag actcctttca
   170941 tcgacaacgt gagaaaattt gacaaacgta tacaaatata cgacgtagat gccgaaagga
   171001 tatattcgaa agacaagaag acgattctgg ccgatacttt tgctttgtgg agaatagtag
   171061 atccgaggaa attcatagag acaatgaaat cagaactgac tgctcttaca cgaattgatg
   171121 atgtagtgta ctctcacgtg cgaaacacat ttggaaaact cgattacgac gagatcattt
   171181 ccggaaagag aacggatgtc cttgatgaaa ttaccgctct cgctgcaaat gatatgaagg
   171241 acttcgggat tcaaataata tccgttcgcg ttaagagagc cgatttgcct gacgaaaata
   171301 ggaacgccgt cttcgagaga atgaagtcgg agagaataca ggaggcttca ctgataaggg
   171361 ccgaagggaa tagagaggca cagaagttga gagccgaggc ggataaagag gcgcagatta
   171421 cgatcgccac agcccagaag gaagcagaca taataatcgg aacaggagat gctcgagcgc
   171481 tttcaatcta tgccgaggcc ttcaacagag accccgattt ctatgaattc atgaagagac
   171541 tcgaagtcta cgagagcact ctagaggatg cgaactacat tttgggtcct gccatggatt
   171601 tcattgacaa actgtccaaa ggagaataat gagctgtaag aagtctcttc ttgaacctgg
   171661 acactttgag atcaatgtca gaagatccag gttcataggt gacagcgcaa gatgcgaatg
   171721 tgaagaagaa gcaaagtcct ttatcaagtc gctctctcaa aggtatgcga gtgcggacca
   171781 ttgttgctgg gcataccgga tttcccttca ggggagggaa atcgctaatt actcggatgc
   171841 gggagaacct catggatctg ccggtaagcc tattctcgga gctatcgaaa gtcttgagct
   171901 tacggatgtt gtcgtggtag ttaccaggta ttttggcgga acaaaactag gagtgagagg
   171961 tttgatcgat gcctacggag agactgctgc aggagcgctc agggccggga aattggccct
   172021 gtattgccct gggctagagt tggttgtgga aatgacttat agtcaatgga acgattttat
   172081 caggctcttt ggtgaagata aagacttttc ggtccgaaaa gtcgactatt cagaaagggt
   172141 ttttgcatca cttgctgttc aggaaaagaa catagaaaga attacggggt ttcttgaaga
   172201 cagaagaatc tttcacaaat cgggggaaga actaagcttt gtcagcccgc tcgagtgatt
   172261 ctgattaatg tactcctgca agtaatagtc cattatcttc ctggatatct gtgatgccag
   172321 tccggaccca tagcctcctt catgaacgaa gactgttacc actatttcgg gctcctcaag
   172381 tggcatgaag ccggtaaacc aggaatgggt aggttttgag cctccctgtt ccgctgtacc
   172441 agttttgccg gctgccgttc cttcgaaatt tctgaaggcc gaatatgcgg ttcctgcctc
   172501 ggcagctcca cctcccttgc ttatgacatt tctcaaagcc tctgtaagca agctccagtc
   172561 actttcacga agctcaatta ctgtcctgcc actcactctc tctgcgccaa tatagggaag
   172621 tatttcgcgg cctttggtag caatttcacc ggtcatcttt gccagctgta agggagtgat
   172681 gcttagataa ccctggccta ttgaaatctg aatagtatcc ccgggaaacc agttttcttc
   172741 cagtttttct tgtttccatg agggacaagg cattagacct gttgattctt caggtagatc
   172801 tatgttagtt ggctcgaaaa cctcccaaat cttggcataa ttgctgatcg tgtcaattcc
   172861 caactcaagc cccagctgat agaagtatac gttacatgag acggttatgg ccttagacag
   172921 atcagtaatg ccatggccgt aaagattcca gtctctgtaa accccttgaa ctatgccctg
   172981 actgtctttg tactggaaac ttcctcgaca atcgatttgt tcagttgcat cctttccttc
   173041 tgccaaagct gccattgcaa tgaacggctt aatcacagat cctggcgtaa aaggagttag
   173101 cgctctattg acaagcggtc tggagggatc gagattcaat ctttcccaat ctctgttgga
   173161 gattcccctt gtgaaagaca tgttttcgaa gccggggacc gataccaggg caaggatttc
   173221 tccgcttgtt gtcatcacta ctgctgcacc gctcttacct gtttcgctga ttaattcata
   173281 aatgtatttt gacatttgag agtctatggt tatagtaagg tcttttcccc ctacaggcgg
   173341 cctttcacct tcaagactta tctgacgtcc taacgcatcg actcttatta actgctctcc
   173401 tggagttcct ctcaacaagt cattgtactg cttttctatt cccattaccg gttctccgtc
   173461 agcttttatg tatcccacaa cgtgcgcaac accgggattg ttttgatagt atctgataac
   173521 tctttcagaa acctccagtt ccgggaagtc gttaagcctt tctgcgagag ccgctggaag
   173581 actcgtttcg acacttccaa acatctcaag gtttctcaca agcgtttctg ctttctgttt
   173641 gtcgtcgaag gcatcaatta tcattctctt caggtcatca gggagaaaca ccaccctttt
   173701 ttcaatattg tagtagggtg catcccacgc aagcggaatt ccatttcgat ccaaaatcat
   173761 acctctttga ggaagcaaag agatcgatcg atggctaatg ctttctactt gtgccgcgta
   173821 tttatcagag ctcacaattt ggtaatcgta caatttataa atgaagagtc ccactgttag
   173881 aatgaaaagt acgaagaaga ggtcgaatct cttaatcttc aacgctcaac ctcctcaata
   173941 tgagaaatgt tgcgaagaac gggaagagta caccgaagcc ggtaccggca aaacagactc
   174001 ctgtgattcc tattgccgcc gcaacgagag caccaaaact tggtgttcga taacggtcct
   174061 gaaaagcaac tgcagaaata acaagtatta ttgcaagggc gattaccata ccgattccgg
   174121 aaagtgcaat tccaactatc catgaaagca gtaatgcaag tgaaagcgca aatgcgacgt
   174181 ttctcgtcgg tagaaatacc aggagcgtta gatatatggg atataaaggg atcaagtctc
   174241 cgaatccgag atcaaagaat agagccggta ctatcagaag cagccaaatc attcctgcaa
   174301 ctccagaaag agaaagtagt caccaaatac gttcggattc tttaccaggt aatcgcttcc
   174361 atggcgacct gcgatttcgc ctattagatc gagcttcatt gctttgtagt atccgtaccc
   174421 tggaaccgtc gaagcaattc tgactgaatc acccgcctct agataatacg gatcaaatgt
   174481 tgagaaatac agttttcccg cgtctgaata gattacgcct tcgataactg tctcctctct
   174541 gtgaacagcg gccgaaattc tagttcccgg agatgtgagc ggagcaacaa gtaagtagcg
   174601 ttcatttgag ttcttaacca caccatagat ggtcgatgaa aagaacgaag caacgggagt
   174661 tcctggcaaa gccggtctgt tagtggtcat aacggctgta ttgtttcttt caaaactgaa
   174721 aaacgcaact ctcatcggaa ttggaaactc aaacttgtca ctgaatgaat cggcatatct
   174781 attcagatct cttattacac gatctatgct tcctgtttga aagagcgccc tgaaaaagtt
   174841 ctcgatatcc actctcagat ttataagggg taatgccatt ccatcaaaaa actcactcgc
   174901 tcttctgttg gtacccgtca cttctagaat cgatgacagg acaagaaaga tcattaagat
   174961 tacaaccgcc acttcagcaa acttcactag agaagtctcc tctatctcaa gttgcctttt
   175021 tcaattcttt caagtatgga gactctctcc agaacctgac cagctcctct cgccacacaa
   175081 gtcaagggat catcggctat gacaaccatg attccagttt ctctttgaat cagttcctgc
   175141 attccgttta agagggaacc tccgcccgca ataacaatac cgctctgcac gatatcggaa
   175201 atgagctcag gaggtgtgtt ttccacagtg agtttgactg cctcaacaat tctagaaacc
   175261 ggttctgcaa tcgcctctcg aatctctgaa ccggagatgg aaatcttacg cgggagacct
   175321 gaattcaagt ctagaccaac aatttccgca taggaaccgt cggcttcttc tgtttcgacc
   175381 acatttccaa tttccatttt gacacgttcc gctgttttct cgccaatcat cagtctgtac
   175441 ttttctttga tgtactgaac gatataatcg tccatttcgt ctccgccaac tcttatagat
   175501 tggctcaaga caatgctgcc gagggaaatc accgcaattt cagttgttcc tccgccgata
   175561 tcaactatca tattccccgt aggttcttcc acattcaggc cggcgccgat tgaactagcc
   175621 attggttctt caattaggaa gaccttcttt gctccagctt ctattgcggc atccacaatt
   175681 gcctttctct ccacttcggt cgccccggaa ggaacgccaa tcacaattct tggtttaaca
   175741 aaagttcgtc tgccaatagc tcttcccata aaatacttca gcatcgcgag ggcaatgtcg
   175801 taatctgcaa ttacgccatc tttcaagggg cgagtagcaa ctatactggc aggcgtttta
   175861 cccaccattc tttttgcttc caggccaact tgaaggatct ttcctgaatt tacatcgatt
   175921 gcaacaaccg agggttcatt agttactact ccttggcctt ttacatagac cagagtgttg
   175981 gcagttccca aatctatacc catgtcttgc cttaagaaca ttcgaaggcc tcctgtcggg
   176041 aaagtcttta ttctcaattc taccatcagg atgaggccga atgacgaagc gtgtgaaatt
   176101 ccacgtcatt gttcaataac cttctggaga aggtattata caagtttgac tatgccttaa
   176161 tcaaagtgat agaatccatg tggcctcgcc aatgacaaag taagggccaa ttattcacta
   176221 ttctagagct agatgaacta tttttttgat gaagttcgat gagatgattg tgatgaaaac
   176281 acggaggtga agacatggaa gatagagagt taagaacgtt gaaccatctc atgtacatgg
   176341 aatactacac aggaggaaat gcaggactct tcatgaagat gaagcagctc gtgtattcat
   176401 tccagagtcc ttttcagaga atagatattt tcgaacaccc tgagtttgga atggttcttg
   176461 tgctagatgg gataacaatg tttactgaag ccgatgaatt tatgtatcat gaaatgttag
   176521 tccatgttcc gctttttaca catccgaacc caaaacgggt cttgataatt ggtggtggcg
   176581 acggtggtag cttgagagag gtcctcaaac acccctctgt cgaggaagcg attctatgtg
   176641 aagtagatcc atatgtaatc gatgcggcta agctccatct gaaggaaatg tcggtggaac
   176701 tagacaatcc taaggcatct atctttaatg aaaatggagc ggactttgtg agaaagaaca
   176761 aggactattt tgatgtaata attgtggact ctactgaccc aacggcaggc gaaggagggc
   176821 atctctttac caaggagttc tattcaaatt gctacaaagc tctaagaaag gacggaatat
   176881 tctgtgcgga aactgaagac cctttctatg ataaggggtg ggttgcgatt gcttataaca
   176941 ggatttcctc tgtatttccc atttcaaaga tgtacaccgg tttcatgacg acttatccct
   177001 ctggtatgtg gacttataca attggatcta aagaactgga tccactcgag gattacgatg
   177061 aagaaagggt tcttgatttt ggagcgcccc ttagatacta taatactgaa atacacaggg
   177121 cctcgtttgc tcttcctacg ttccttaaag agctgaccgg tcagttttga tatacggcag
   177181 cgaagcttta catttgatgt attgtgaata ggatttttat agttgagaga atgacctcca
   177241 gtattttgct ggagttgttc tcccttcttt tgttgaattt aacctggttt acccgtgccg
   177301 tgatagaatc atccttgatc ggcaggaggg agtattaatg tataagagag atcgtattag
   177361 gaacatatct ataattgctc acattgatca cgggaagacg actctcgtcg acagattctt
   177421 ggatatcacc gatactgtgg acagacgaaa caaaaaggat cagttcatgg attccatgga
   177481 tattgagaga gaaaggggaa ttaccataaa gtctaagccc gtgaagctga attaccttgc
   177541 tgatgatggg attacatacg agatcaacat tattgatacg cctggtcatg ttgacttcaa
   177601 ctatgaggtt tccaggagct tggctgcctg tgagggagcg attttgcttg ttgacgcttc
   177661 tcagggtgtt gaagctcaaa cggttggcaa cacatatcta gcaatcgaga atgatcttga
   177721 gttgatacct gtactgaaca agatagatat tccaaatgca aatatcgagg aaacacttgc
   177781 ggaaattgtc gatcttatag gctattctcc tgaagactgc cttcaggcga gtgcaaagac
   177841 tggtcagggg gttaaggaga ttctggaagc agttgtcaag agagtctcac ccccgtcggg
   177901 aatgctagaa gcaccattaa aggcattgat tttcgatgcg atttacgata agtatagagg
   177961 cgtagttatt cacgtaagaa tctttgaagg tagtgtcaaa gcgggggaca gaattcagat
   178021 gatggcttcc ggtgaaatct ttgaagtagt cgaagtagga tacttccttc ctcagatgga
   178081 aatcaccgat tctctggatg caggtgaggt tggttatatc attgccgtaa taaaagacgt
   178141 atcgagtgcc aagataggag ataccgttac ttctgccaat aatcctactg atactgccct
   178201 tcccggttac aaagaggcga aacctatggt ctacgccggg atgtttcctg gaatgcccga
   178261 atactacgac gaactgagaa aggcgctgga taagttcaaa cttaacgatg cctcgctcgt
   178321 ttttgaaccg gagaactctc ccgctttggg ttttggcttc agggtggggt tccttggcct
   178381 acttcatatg gatgttgtta gagagaggct cgaaagagaa ttcgaaatag cctgtattct
   178441 taccgcccct aatgttgttt atcgggtcac tttacagaat ggtgaagttg cagaaataac
   178501 taatcccgct agctttccag agcctggtga gttcctgaga gttgaagaac cgtttgtcga
   178561 cctatctata atcacgcctt cggagtatat gggcaaccta atagggttca taacttccga
   178621 aaagagaggc gacttcaaag cggtggaaaa cgctggaaag aatcgggttg ttctgcgttt
   178681 tgaggctccg ttgtcggaga taatgtttga cttcttcgat aggatgaagg caatttcgag
   178741 aggttacgca tcaatggatt atgaaattct tggctatcgt gagtcggatt tggtgaaagt
   178801 gacaatcctc gtcaataagg agactgtaga ttcgctttca ttcatagttc atgtagacaa
   178861 ggaatattct gtagcaaaaa aggtagtcga caagttgagc gagctaatac cgcagcatca
   178921 atttcagatt ccgatacagg cgaaatcaag aggtagaata attgctagaa gcgatataaa
   178981 ggcattacga aaagatgttt tggccaaatg ctatggagga gatgtcacaa ggaagatgaa
   179041 gctccttgag aagcagaaag aaggaaagaa gagaatgaga gaaatcggtc aggtaaccat
   179101 tccacagaat gccttcctgg cgattctgag aatcggtgaa gaagaatgac gaggtgagaa
   179161 tgttgataag acttggggca catatgtcca tttctaaagg gttcgataaa gttcccgggg
   179221 acactgtagg aatcggcgga aacacttttc agatctttcc tcacagtcca aggatgtgga
   179281 aagcctctct tcctaagagt gaaatgtcag caagtttcat atctgaaatg aaagacaaat
   179341 ctctagatcc tttcgattgt atggttcatt caggttatct cgtgaacatt gcttctccta
   179401 atgacgaggt atgggagaag tctgtgaacc ttctctctct cgagatgaaa atcacagcta
   179461 ctttgggttt gaagtatttg aatttccatc ccggaagtca cctgggcgaa ggtcttagtg
   179521 aaggactaga aaggattctt aaaggagtgg aaatcgttct aggtgagaat cgggacaacg
   179581 atgtgatgct tcttctggaa aacgtttctg caaaaggcaa tcacattggc agcagttttg
   179641 aggagcttgc agcgataatt gacggttctg ttcagcctga aaggattggg ataacatacg
   179701 atacctgtca cgggtttgac tcgggtttcg acataaggga cttagaggga accttgaagc
   179761 ttattgacaa aatcgattcg acggtgggct acgaaaagct gaagatgata catctcaacg
   179821 acagcaaata tccgctcgga gctggcaagg atcgtcatga aatgattggg aagggttaca
   179881 taggtatgaa tggcggattc aaggagtttc tttcaaacga aaagatacag gaaatcccgt
   179941 ggcttcttga gacgcctgga gacgatgcag atcatgcttt agaaattaag tatgtgaggg
   180001 agcttattgg atagcggagg aaagcaatcg atgaaaaagt atgttgtggt aacaggaggc
   180061 gtaatcagtg gcatcggtaa ggggatcctt tccgcctcaa ttgctagagt tatgaaggag
   180121 tgcggagtag aggtgaatac tctgaagata gacccgtatt tgaacctcga tgcaggtacg
   180181 atgaatccaa accagcatgg tgaggttttc gttaccgaag acggttatga agcggatctt
   180241 gatctcggcc actatgagag atttcttggc aagaacatga gaagagagaa taacatgacg
   180301 gcggggcagg tcttcaaatc aataatcgac aaagagagga aaggtgactt tttaggagcc
   180361 accgttcaga tggttccaca tgtaactgat gagatcaagt ctagaatcag aaaagtgccg
   180421 ggtgatctca taatgataga aattggaggt actgttggcg acattgaagg agagatattc
   180481 cttgaagcgg tacgagaact gtgggtcgag gaggggccgg agaacttcct cttcatacat
   180541 gtaacctatg ttccctatct gcgtgtcacg aacgagttta agactaaacc aacacagcag
   180601 tcagttcaac tcctaagaag aattggaatt cagcctcaaa tgattgttgt tagatcagag
   180661 cttccaattg aagaaaacga actcggaaag attgcccttt ttggcggagt agaaaggcag
   180721 atggtttaca acctacccga ttcagaaaac gtgtatgatg ttcccagaat tgtttactcc
   180781 catgggattc acagaaagat cgcatcctgg ttaaacattg aaatcgaaga acggttcgaa
   180841 tggaactatc ccaagtcgtt tgctccaacc agggtagcaa tagtgggcaa gtaccttggg
   180901 acagacgatg cttacaagag catttctgaa agcattactc tctgcggagc aagcaagccg
   180961 gaagtcattg attcacagtg ctttgaggat atgacggatg cacagataaa ggccgagctt
   181021 cgcaaatacg acggaataat catacccggc ggttttggca aacgaggtat tgaaggaaag
   181081 attgccgtga taaaggcggc cagagaagag gacatcccga ttctcggtat ttgtttggga
   181141 atgcagctta tggttatcga gttcgcaagg aacgtttgca agttagagaa tgcaaactcg
   181201 acggagttcg atccttctac tccatatcca gtcatcgatc taatggaaga gcagaaagca
   181261 atattgaaac ttggcggtac gatgagactt ggcgctcagg agactccaat agaagaaggt
   181321 acaactcttt acgaggcgta taacgagcgt ctcgtctatg aaagacacag gcacagatat
   181381 gaggtcaacc tcgagcgttt tcccgatctt tttaggacgc cagaagaaac ggatgaaagt
   181441 aaactgagaa tatcctctag agcgacattc gtcgaggcga tagaacttcc tgaaaagaga
   181501 ttcttcgtcg gtattcaata tcatcctgaa ttcaggtcga aggtgggtag gcccaatccg
   181561 atatttgaac tcttcctgac gagagtaaga gagaagagaa ggattcaaca gtgacattcg
   181621 aaaacgggag agtgctagat ctttcggaac cgcgaataat gggaatactc aacattaccc
   181681 ccgactcctt ttttccggga agcagattcc ttgacatacc ggttgctctg gataaagctc
   181741 tcgaaatgct cgaaaatggt gcagcaataa tagacatagg tggagaatcc tcaagaccgg
   181801 gagcagaaaa ggtagagtca aaagaggaaa tcgcaagggt tctaccttta ataaagcaac
   181861 tgcgaagcaa aagacctgat gcagtgatct cagtagacac ttataacagt gagactgcga
   181921 gtgctgcact ttctgaaggg gctgacatga tcaacgatat caccgggctc aggtcgatga
   181981 agatgataga ggcagtctct gaaaaccatg cagctgttgt tattatgcac atgagaggcg
   182041 atccttctac catgagtcga atgacagatt accgagacgt agttggagaa gtttcatcct
   182101 ggttacttga aagggccagg cgggcaatcg atttcggagt agatcctgac aggataatac
   182161 ttgatcccgg ggtaggtttc gccaagtccg ctgaacaaag tattgagatt ctgaaaaaca
   182221 tagagaagtt taagaagatg ggtttccctg ttcttgtagg gcattccaga aaaagcttca
   182281 tatcgaagat tactggaaca ccggttgaag agagattgga agaaactatt agcatttcaa
   182341 catacttata ccttaaaaag gtaaacataa ttcgcgttca tgatgtacag gagcacggtc
   182401 gaatctttgg tatacttaag acattgaacc catgcggaga gtattggggg gagtgatttg
   182461 ccttatagta gacgctcaaa gaaatactgg aaggaaacct ggaaggcgta tctttttctg
   182521 ctgccttcga tcataattct gggagtgttt gtcttctggc caatcggttt ctcgcttgtt
   182581 ctcagtttct ttaaatggga ctacacatcg accactcgtt atttcatagg ttttgataat
   182641 tacaaagagc tcttcaggct aacctatccc gtttccctaa gttttgtgaa ctcgctaatg
   182701 aacactgtcg tttatgttgt tatcgtcctt gtagtagttc aactaatcta ctatcttctt
   182761 ggacttatga gtaagtatga taagacagac aagagtcatt cgtttttata tgttattttc
   182821 attgctttgc tttccggata tgttcttttg cgtaatacca gtatcggtct cacaactatc
   182881 gggctgtttg cagggttggg cttgatgggt tttggactct atgtacttct tcgcaggaag
   182941 agtagcattc agagcagaag gatgaaatca agccagtggt caacccttat cctgtttatt
   183001 gtcgtctatt acgtttttgc ttaccagatc ctcactcaga gcgctgactt tgttcagtat
   183061 ttcactctgg caaaagaaag ctcggacttt cttaagtcta tctacaatac ggtctattat
   183121 gttctgctgt cagttccaac tcagatagcc ctggctctga ttattgctat gcttttaaac
   183181 aagaacatta agctgagatc ccttttcagg actgcttatt tcatcccttt tgttacatct
   183241 gttgttgccg tttcacttgt gtggcagtgg atgttcaacg accagtttgg attgctcaac
   183301 tacattcttt caatcttcaa cctgccgaga attgcctggc taaaagatga agtttggaca
   183361 attccaacga tagctatagt ctctgtatgg cagcatgttg gatacactac cgtgatcttt
   183421 cttgccggtc ttcaaaacat cgataagtcc tattacgagg ccgccgatgt tgatggcgca
   183481 agcgggtggc agaaattcaa gttcattacc tggccgctcc tctcgggcac cactttcttc
   183541 ataatgataa tcacgatgat aggttcgttc aaggtattct ctcagatctt tattctttac
   183601 caggggctac ctgggccggt caataaaagc ggattgaccc ttgtatacta cgtatttgat
   183661 gcattctaca atcagcagag aatgggagta gcgagcgcgg cagcatatgt gctctttatg
   183721 attattctgg tcttgacaat ggtgcagcta aatgtgggta agaagcgcgt tcactacgaa
   183781 ggttgaggtg ttcagatgac tacatcgtcg actaagatta tctcgaaaat tctactctac
   183841 gtagtaattg tcggtggagc ctttctgatg ctgattccat ttgcctggat ggttgcgacc
   183901 tccttcaagg cgcccagtga agtttcgact tggcccccag tatggacgac aaagaatgca
   183961 gcaagttcct tcacgttcaa gacacaaatc aagcagaaga gcgcaagtgc ttcagtagat
   184021 ctctcggctt tgagtctcag cgaattcaga aacttcgccg cccttatgga ggagtctgca
   184081 agttttgata cagtgaccgt taatcttgat gatgacccca ttaggagagg cgaaatcgag
   184141 atccagctct tggagacaaa cggttctcct gccgactatt cgacggaggt tgatcggcag
   184201 agatttgaat ctctaattga acggtatcat acacttgcta ccaatttccc ggactcagtt
   184261 tcgagagatt tgaaagatct tcctgtagat gatgttggcc gctttctgga cagcttcttt
   184321 aacgtggctg tgtatggaga caatggtttt gttaggcgag ttattctcgt aacgtcgctc
   184381 aaaacggagt attcgaaagc aatcgaaaga ctggcgcaaa gcgttgaaac ttcaatgaaa
   184441 gagttgccaa tagatactga tgaaagcaaa gcgatcaaaa agagaatccg ggaagagtcg
   184501 gccagacttt tcgagaaccc ggtagctgag cttacgcgtt caatagagaa tctcgagcct
   184561 tatagaactg ggagtactgg ggtcaccgat ttgaccgaac tggagaaagt ggttgaaggt
   184621 ttgagagaca gcataaatac tatcaggaat gttgcttctg aagtgatctc gctttctcaa
   184681 gaaatctctg ctggagattc cagagtctct ctttcggtga ggcagctcga aagatctctt
   184741 gaagccgtac cttctggact gattcagtgg atggacttgt tgaatctgta ttctgacttg
   184801 agaattttct acgacgaatc acagaatgag agtctttcaa gcgcttcaat agtgggaaag
   184861 atacggaccg attctgaagt gcactctttg ctgtccgact ttgtgaatcg gtgggatatt
   184921 gatgaagaaa tgagaaccca cttgcttacc aaggttaatc cgtctaatgt acggaacgca
   184981 gttgcaatcc tcttgaacta tcttgacagc aattttaagg agagtctttc aaaatacttc
   185041 tcggatgtgc agacaccact cgaagcaatc aatgatgcca tgaatttcct gagaacttcg
   185101 cttctggttg cttctgatcc agtcctcgat gagaaagtca atagcgcgat ggatgaagag
   185161 acaccttatt cgagtctcat tacactgatt aaggaagtgg cgtcagcggc aggtcaaacg
   185221 attggagtcg ggggcgttgt agcgagcctg gataggcagg caaacatcag tggccacgaa
   185281 ccgtttgcag aattgataag acgaagatgg agagagaccg acttaatagg tactttctcc
   185341 agagtccatt ccgatatatt cagcgaactt gatctctcaa gtaaacccga tgaagttgaa
   185401 agagtttact acaggggtat catttccccg tccggtgcga aatctttcga cataaagctg
   185461 aaaggaattc ctgcggtctg gctcaaagac gacgtatccg ccggcaaggc gaatttcact
   185521 ctgggagaga ctattagcaa cttctttcaa aattatgtca cagcatggaa cgctgcccct
   185581 ttcggcaggt attacttcaa cactgtattc gtggctaccg tgacaacatt tgttgagata
   185641 atctttgcag caatggcggc ctttgcattt gcaaagatgg agttcttcgg gaaaaatctc
   185701 ttgttcactc tcttcctggc gacaatgatg gtccccggag aggtgctcct tgtgcccaac
   185761 tacatcacgc ttactgctct tggttggatt gactcgtatt acgccttaat tgttccgtgg
   185821 gtagtttccg tttttgccat attcttgttg agacagcatt tcatgactat cccgaacgaa
   185881 ctatacgatg cggctatgat tgacggtctt tctaagtggc gctttctatg gacaattctg
   185941 gttccgcttt ctaaacccgc tgtcataacc gctgcgcttc tgaaattcgt cggtagttgg
   186001 aattccttcc tttgggtcct tatagttacg aagagtcccg agatcaggac tttaccggtt
   186061 ggacttcaga atttcagttc tgcaacggga agtgattacc atttactgat ggcggccgct
   186121 acattttcca taattccggt cgtaattctc ttcctgatta cccagaagta tttcattgcc
   186181 ggaattgcca ggagtggtct caaataaaaa gcaagaagaa tagggcgaga tttccgcttg
   186241 gattgttcat ctttctggcg ctgctttttt cgctcctgtt aatcaccaac tataacgcaa
   186301 tagcacgtta caagcaggct cagagaaaac tcgagaacct ccagagggag tatgatgctc
   186361 tcgagaaaga aatcgaaatc aaggaatctg aactggagct cttaaggcaa ctggctccgg
   186421 gaatgccgca aaatgacact ggaggaaata gatgagattc gtagtaacaa cctctcacaa
   186481 gcctactaga gaagaagtcg tgaatgccag aactctggcg gaggaactgg gagtgaagta
   186541 tgtttcgaga ggccgactta gggaatttga gaaagagagt cccgttgatt tctattacgt
   186601 gtttgataag aacggtcaac tttccataaa ggccggagaa tctgttttct tctttcaccc
   186661 aggaatgtca aaggtcagat acaagaacat caagcttcaa aattctgact acctgataaa
   186721 cagcatggag cttcatggtg aagagatagt ccttgacaca acttttggac tgggaaacga
   186781 ggcgcttctc attgcacact tcttgcctcg gggcaaagtc gttggccttg aagcgtccga
   186841 acacatattc agagtagtgt cgcacggcct tcgaaactac ccttacactg atgaatggat
   186901 aagagaggca tcaacaagaa tcgaacttca caatattgat ctgcgggagt tcgtaaagtc
   186961 ttgtgcagac aactcttacg atattgttta ctgtgaccct atgtttgaca gaccccagtt
   187021 gagttcccat tcaatcaatc cgctaagacc ctttgccgta tatgagagaa taataaggtc
   187081 tgatgtggat gaaatgatac gggttgcttc gaagagattc ataattaaga gcagaacgag
   187141 agacacgctt ttcgaggagc ttggtcttca cttcgataga ctctggggaa gtaagaaaag
   187201 tggagtatta tatggggtga ttgaaaagaa atgatccctt tagtactagg tccaactgcc
   187261 gtcggaaaga cttcacttct gctggaaatc gctggaagac ttccgataga agtgatttcc
   187321 gtggattctc gtcagattta tcgcttcatg gatattggta ctgcaaaacc aacacgtaca
   187381 gaactatctc tgcttaagca ttggctgata gacattcgag atcccgatga gagtttcgac
   187441 gtgatggaat tcaggaagct ctctctcaaa tctatcgcag agattaaatc aaggggtaaa
   187501 atccctgttt tggccggtgg gacgggcctg tatgctgacg ccctgatcag aggattggcg
   187561 gatgttccgg ctagagatga aactattcgt gaagccctgc tacaaatcga atcacaaaaa
   187621 aaggggttct taagagagct gctccggaaa gtcgatccca aagcctttga aacgatacac
   187681 gaaaatgatt tgaaacgtac tgtcagatat cttgaggttt ttctcagaac agggaaaccg
   187741 ttaactgaat tccataaaga gagcgagatg tcgggagagt ttggtatagt gattttacag
   187801 agagaaaggg acgagctgca cagaagaatc gaggcacgtg tccgcaggat gattgactct
   187861 ggtcttgcag aagagacgga aaggcttctc gagatgggtt atgctccgga actcaacgca
   187921 ctgaagacaa taggttacgc tgagatggta caatatataa agtgtggagt atcccttacg
   187981 gataccagtg agcggatcat cgtaaactct agaagatacg ctcgtcgtca gattatttgg
   188041 ttcagaagat acagagaagc tttgagaatt gacctgtcca ctctcggaaa tgaggcggct
   188101 caggttcttg aaaatacgat tctctcagtt tgggggggta agaatggctg aaaagttcaa
   188161 tttgcaggat cgtttcttga atcttttaag ggttaaccgg atcgaagtca agatctatct
   188221 cgaaggtggg ttccagacga aggggatggt caagtctttc gacaatttca ctgtgctgct
   188281 tgaagatggt caggaacaga ctcttgttta caagcatgct atcaagatga tggttcctca
   188341 gaagtatgtg aagttgacga atacttcggg caagaaggag gactgaagag agtatagaca
   188401 ctttacttgt cgctgttttt tcgtctgaag aacagaatat gaaaacagaa atactgggcg
   188461 agcttcaaga gctcgcccga actgctggtt attatgtaac ggaaaacgtc gttcagaatc
   188521 ttgattatcc cgatccaaga cactaccttg gaaaaggaaa ggttgacttc gcgaagagaa
   188581 tgattgaagc ctttggtgta tctcttgcga taacacgaca tgagttatca ccgtctcagg
   188641 cgatgaatct tgaaagactg ctggggatac ctgtcataga cagaacgcag ttgattcttg
   188701 aaatcttcgc tgaacatgca acgacgaaag agggaaaact tgaagtagag ctcgccagcc
   188761 ttaagtatca acttccgaga ctgaagggat ttggcaggat gctttcacaa accggagcag
   188821 gcataggcac aagaggaccg ggcgagaaga agcttgagat agatagacga caggctcagg
   188881 acaggatttc ccgcctcaga aaagagatcg aagaactggc gaaacggaga gagatatcac
   188941 gcaagaagag acagtcatct tccgtacctc ttgtttcttt tgttggctat acaaatgttg
   189001 ggaaatcatc gttagtctcc gagattagca gcgaggatct agtcatagag gataagcttt
   189061 tcgcaacgct cgatacccga gtcaggaaag cgaagttgcc ggctggaatg cagttactgg
   189121 tttccgacac agttgggttc ataagagaac tgccacacga gttgatggag agcttcaaat
   189181 ccacgttgga cgaagtgaag tactccgata tcctcgtggt agtttccgat gcatctgaca
   189241 tggcaattaa agacaagtac tctgtagtag accgtactct acgtgaaata ggtgcgggag
   189301 agatcagaag aattcacgtt ctgaacaaga tcgatctctg tacgaatgaa agactggccg
   189361 aactggaagg attctttcca gattcagtaa tggtaagtgc attgagaagc tacaacattg
   189421 agggcatact taacgaaatc acaaggaaac tctttggtga aaagtcgaga agaacgttga
   189481 gattaactcc tgccgagttt tcgaatttca tgagatttag aaactccgtg gaggtgcttt
   189541 ctgagagctt tgacagagag ctcatcgaga tcgaatattt gtcctccgat gagataaatg
   189601 aaaggctcat ttcttctata tgtgaggagg gaagaaaatg aagaggttat tatcggtcat
   189661 actggtaatt ctgactactg gaatgttgtt ttcggtgaca cttctttttc ctttgaagag
   189721 ggcaccggag ataacgggct attttggcga gtttaggggg aattccaaaa atatcaacta
   189781 tcccgagcat ttccacatgg gaatggatta ctcaactgga agcattgtgg gacttgatct
   189841 tcgttctcca gacgattcgt atgtgcacca gatctatata aaccatccta tatatggcat
   189901 tgggatagcc cttacgcttc ccgaagtcac gaatattctt actaatgagg aaggtatcaa
   189961 tgtgatcttc gcacacgtga acgaagtcgg ggacacttct acgttaacgg gaagaaaact
   190021 aaatgatctt tatcaccagc taatttctga gtttggagat cagtatgtcg aagtaacctt
   190081 tgaccctaga gagcttccgt tcagaaagag cgatgttgtc gcaaagtcag gaaacagcgg
   190141 taatgttcct cctcatcttc atctggaggt tagggattca acaatgcaga cgataatcaa
   190201 tccgggattc tttttcgata caggttctcc aactccagca attgaaatac ttgacataag
   190261 agcgggcgga aaaacgtact cattttcgga aggtaaacca acgatagaaa tgactccgaa
   190321 cactccactg gatcttcacg caaaggtgca gctgaggcat ccgataagtc ccaagactat
   190381 tgagctgtac gtagagaaca gtttgatcta ccagatagat ttcgtatcat ttgaattgga
   190441 cgaagtcgat aaggttcgcg aaatatattc atcgccatca acggaatcgg attattggtt
   190501 caacttaaac tcgaggattt cgctcagtgt cctgccaata aacatctggg atgacatcga
   190561 ctggacaaat cctagagatg caagggtggt agttcgagat cactggggga acgaggcttc
   190621 gaaagacttc agaattgaaa tgaggaggta attcagatgt ttttcaagaa gaagccgcgc
   190681 gatattggca ggaatccctt ttacgacaac gggaacactg tggttgtgca ctttcagtgc
   190741 aacaagtgcg gagagaaatt cagaagccat cttagaaagt attacgatgt cagtgtaaac
   190801 tatggaaaag gtaagggtgc ttacaggctc gacaaagagt tcattggcag caactgtcag
   190861 aacaggattc agatcactgc tgaattcaat agagctttca aacctttgtc tttcgaaatt
   190921 gttggcggta gattcatttc caaagaagaa tatgaagaat agattcattt tataggaggc
   190981 agttatgaag acttggttgt tcaccagtga aagtgttacg gaaggccatc ccgataagat
   191041 ggccgatcag atttccgatg cgattttgga tgcaatgtta acacaagatg agaactcgag
   191101 ggtcgcagtt gagactcttc ttgctacagg ggttgcggta gttgccggag aggtaagtac
   191161 aaaagcctat gtcgatattc cgagagttgt cagagataca attctcgata ttggatacaa
   191221 cagggctaag tttggtttcg atggggaaac ctgtgcagtt ctcacaagta tagacgaaca
   191281 gtcaccggat atagctcttg gagttgacag gtcgtatgag gcaaagaaga atgatgtaga
   191341 tagatacgca cttattgggg ccggtgatca gggtatgatg tttggttacg caacgaacga
   191401 gacgccggaa atgatgcctt tgcctatcgt tctcgcccat agacttgcca gaagactcag
   191461 tcaggttaga agggatggaa ccgtccaagg cttcaggcct gatggaaaga cccaagttac
   191521 cgttaagtat caagacggaa aaccggttgg tgtgactgct attgtagttt caacccagca
   191581 tgatcccgat ctgaccagcc acgaaatcga aagacttgtc gttgacaatg tggtagcccc
   191641 ggttatcgag catgaactat tgcttgaggg cgtcgaaatc ttcgtgaatc caaccggacg
   191701 ttttgtcaag ggtggacctt cggccgatac tggtttgacc gggagaaaga ttattgtcga
   191761 tacctatgga ggctggatac ctcatggcgg aggcgccttt agcggtaagg atccgacgaa
   191821 agtcgataga tcggctcatt acatggccag atacactgcg aagaacattg tagctgccgg
   191881 attggcggaa cgagtaacac ttcagttggc ttatgcaatt ggagttgcaa aacccgtgtc
   191941 gttcatgata gatgctcacg atacggagaa ggtggatctc gagaaattaa gaaaggccgt
   192001 tctaaaggtt tttgacttca ggcccgccgc cattatagac cgtctcaatc tcaggcgacc
   192061 aatctacaga cagacagcgg catttggtca ttttggcagg attgatgttg atcttccctg
   192121 ggaagaaacc gatgcggtag accaattgaa gaaagcactg gattgatgat acaattaaca
   192181 ggtaaatctc gaaaggagga gaagtgataa gtgccaaaca atgcatcagc aaaaaagaga
   192241 gtacgacaga ccgccaagaa aacaatgatg aacaaggctg tcaggacgaa atttagaaat
   192301 gcttcaaaga agctgtataa agctatagaa gagggtgaag atccaaagct cgtttcgtcc
   192361 atgctcagtg aagtatattc tactctcgat aaggcggcta agtccgggac gatacacaag
   192421 aatacggctt cccgtaaaaa ggctagattg acctcaaaag tgaaaacata cgtcgagacg
   192481 agaggataat acaatcaaaa atgaaaaaag gtggctttag ccaccttttt ttgctgtcaa
   192541 cagcagcaat caaagattct ctggagctgc gtcctgatgt gtccctgcga ggacgttctc
   192601 ttcagtctca ggatcgaaca aatgcatcat cgacatatcg aaaacaagat ccatcttgtt
   192661 gccggcagcc gctctgctct tcgggtctac tttcgccaca atgtcatctc cgtgaatgtt
   192721 tgcatggata agcgtttcgc tacctagagg ttcaacaacg tctacactgc ccttgactgt
   192781 atactcatcc tttggcgcga cggcgtacat cttgtcgtat atatcctccg gtcttatgcc
   192841 gaatgttatg tccttaccga ccaaaccttt aactttgtca accttgtcat caggaaccag
   192901 aagtttcatg tcttctttga cgacccaaac actaccgttt tcggatacaa gctttgcgct
   192961 tatgaagttc attgctggag ttcctataaa ccccgcgaca aacttatttc tcggttcaaa
   193021 gtagaccgaa taaggatcac ctatctgctg aataatgcca tctttcatta tgacaatctt
   193081 gtctgccatt gtcattgctt caacctggtc gtgagtgacg tagatgatcg ttgcctgtag
   193141 attctggtgg agcctcttca gttccgccct catctgcacc ctcagcttcg cgtccaggtt
   193201 ggatagaggt tcgtcaaaga ggaagacttt tggctttcta actatcgctc ttccaacggc
   193261 aaccctctgt ctctgaccac cggaaagctg ctttggcttt cgatcaagta gctgctctat
   193321 tccaaggatt ctggcagctt ccttgactct ttcctcaatc tcgggcttgg gagtcttcct
   193381 aagcttcagt ccgaatgcca tattctcata aacagacatg tgcgggtaaa gagcataatt
   193441 ctggaaaacc atagcgatgt ctctatcctt aggttcgaca tcgttaacga ctttaccacc
   193501 gatttttatt gttccctcgg tgatttcttc caatccggct atcattctca atgtcgttgt
   193561 ctttccacag ccggaagggc ccaataaaac aaggaactct ttgtctttta cttccagatt
   193621 tgcatccaaa acagctttga ctccatttgg ataagtctta cctaccttct cgaggataac
   193681 ttctgccatc ttacacctcc gtcaatcatc atcgacttga acaataagat cctttatttc
   193741 aacatccatc gcttcatcgc cagcgtccat taaatagaga tcataaaccc tctcaagaaa
   193801 cctgttgaag agcacgtact tgcgatagtc catcactaca gccttgggaa gaccattttt
   193861 tgttataatt atatcggcct tctcacaatc gtcaacgacc tttgaaaagt gcgtttttgc
   193921 ttcggccaga gaaaaaaaag agagctcatg taatcttgac aaaggaacca cctcgggacc
   193981 attatacagc atgaccataa ttatagtcaa gctttggagg tgccagagat ggcgaagaag
   194041 ttcttcttga aacaaccaat gatgagaaag gtaatctacg ctctccttcc aattctgatc
   194101 ttcgctgttg gtaatttcgg ctgggtcgtg ttttccaagg tgtttctgtc cattctcatc
   194161 tcggtttttg tcgaatggtt attcgaaaag aaaaagggaa aacccgtcag cgaggccgca
   194221 atagtcaccg gctttctgat tggcctgatt cttccaccgg cagtgccctt ctggattgta
   194281 attgttgcct cttcatttgc gataatcttt gcgaaaatgg ccttcggggg tttcgcgaag
   194341 aatctctaca atccggcctt ggtcggaagg gcctttgtct atattagctt tcccgcagcc
   194401 gttcaacaga gctggacgcc ggcggttcaa agctggggac agggattcgt tcgctggatt
   194461 agctccgata tcattactat ggccactcca gcgaatgtca tccgaaatgg aggaagtgta
   194521 agtgcgtgga gaatgatatt tggcaatatc tccggctcat caggtgaaac agcaaagtgg
   194581 ttgatagcag ctgcagctgt gtaccttatt atcaccaaag ttgcttcgtg gaagataatt
   194641 gtctcgacgc ttttgtcagc atttgtcatg tcagggattg tctatatatt cgacccttct
   194701 aaaatgagcc cgttttactg gattattgga ggtagtatat tgtttggggc agtattcatg
   194761 gccaccgatc ctgtaagcgc tccgaagaaa gaaaagtcga aatggatata tggagctatg
   194821 atcggttcac tagcggtgat aatagggtct ttctctcttt tcggtgcggg tcttatgttc
   194881 gctgttctca ttgcaaacac atttgcgtct cttctggatt accttaacac cccgaagaag
   194941 gtgagggcat gagtgacaga ttgttctcag tagtttttac ctttgttctc acaacggtct
   195001 tcgttttatg tcttgctggc attaacgctt tggcttccgg cacgatcgag aagaatgagc
   195061 aactggaatt ccaaagatct ctcgtttacg tgtttaatct cgcagaagac atcgactcag
   195121 tctcagatga ggaaattcta aggctttatg gcaacaggat tgaagagatc accggggatc
   195181 agacagttta cattgtcaac aaagatggct caaaggagtt agcctttaca gtacagagtc
   195241 cgggcttgtg ggggacaatt acggcactca ttgcattcaa tgaggaggga acgaggatta
   195301 ccggaataga tttcgtcagc cactcggaga cgcccggtct tggaggtaga attgacgaaa
   195361 gtcagttcaa ggagcagttc agaggagaga agatctcttc agatccctct gcaagagtgg
   195421 ctgtggttac gggacaggtt actgtctcta aagaagatgc tcgagttgac gcaattacgg
   195481 gagccaccag aacatctgaa tcaatacaga tcctcttgaa caaggcgatt aattctgtat
   195541 tacccgtcgt tcgcgaggtg gtgaaataaa atggctgatt ccaagaagac ggtctttctt
   195601 gcaaacatct ggaagaataa tccggtaata gtgcagattc tggggatctg ttctgctcta
   195661 gcagtcacaa acaatctgac aaacactctg attatgggag taggtctaat ctttgttacc
   195721 gctttctctt cactgaccat ctccgctctc agaaaactga ttccaagaaa cgtgcgtatg
   195781 atgattcagg ttcttattat tgcaacttac gtgataattc tcgatattgt cctcaaagca
   195841 tatctaccct ctatcagcaa ggctcttggt ccttatgttg ggctgataat aacgaactgt
   195901 atcattatgg gacgagcaga agcctttgcc cagtctcacg gtccgattct ttcctttctc
   195961 gacggcgtgt ccgcgggaat tggctattcg ctgattcttc ttgccgtagc ctttataaga
   196021 gagcttttgg gttttggcac gatttttgga gtgagagtta tgccggaaag tttcactcct
   196081 tgggtgataa tgataatggc tcccagcgcc ttctttattt taggtgcgat aatctggatc
   196141 gcgagaacgc cgatcgtcaa gaagaagtaa ggaggggttt agatgggagt tccacttaat
   196201 ccatttgtaa taatgatagc ggcggcattg acaagtaata tggtgctgag caattttctg
   196261 gggatgtgct ctttcatatc ggtttctaaa gacataaaaa ctgccaatgg gcttggtatg
   196321 gcagtgacat tcgttcttac gcttacttct gttctcaact acatagtcta ctattacata
   196381 ttagttcctc tgaatctcga ttatctaagg tacatagtat tcattgttgt aatcgctgct
   196441 tttgtgcagt tcctggaaat gatcattgaa agattctctc cggctctcta catcaacttg
   196501 ggaatcttct tacccctcat cacagtcaat tgcgcaattc tcggtggagt tctcttcatg
   196561 ataatcagag agtacaacct catccagtct ctgttctttg ggttcggttc cggtgctggc
   196621 tggtggcttg cgatagtcgc actgtctgca atgcgcatga gaatgagaga aaatgatcta
   196681 cctcctcagt tggttggccc aggcattaca gtgataatta tcggaataat ggcacttgcc
   196741 ttcatgggtt tttcgggcat gttgcccgtt caataaggtg gtgttttgat tgcaaatagt
   196801 catatcagct cttgtgatcg gaggaatttc ggcgctgctc gctgcaattc tcactgttgc
   196861 ggatgccctt gtgaacaact atggagaagt tagtataaga gtaaatgatt cgaaagactt
   196921 gaaagtgaaa ggcggagcga ctcttctgtc tacgcttgca agtgagaaga tcttcattcc
   196981 ctctgcatgc gggggtaaag gcagctgcgg actttgcaag gtaagggttt tgacagacat
   197041 cgggcccatt ctcccgacag aactaccata tctttccgag aaagagaaga aaaacaacat
   197101 taggctttcg tgccagatta aggttaagag tgatatatca atcgagatcc cggaagaatt
   197161 gttcaatata agggaataca tcgccagtgt ttcatcgata aaggatgtga cacatgacat
   197221 taaggaagtc ttctttgatt tggaagatga gatcgaattc aaggcaggtc agtatgtgca
   197281 gctgatagtt ccagcttatg gagatatcaa gggtgaaaca atgagggcat attcgatgtc
   197341 ctcgcaaccc tcggttaaga gcggtgttga attgctgata agacttgtcc cgaacgggat
   197401 tgtcacaact tacgttcaca aaatactgaa agttggagac agaattagga ttcttggacc
   197461 tttcggtgat ttttacttga gagacaccga ttcagatata atattcattg cgggtggctc
   197521 tggaatggct cccatcaaat caataattta tgacatgatg gagagagaag tgaaaagaaa
   197581 tgcctattat ttctttggag caagaagcaa gagagatctc ttctaccttg aggagatgaa
   197641 agaggttgaa agaaagatgc ccaattttca tttcattcca gcactttctg atccagtgcc
   197701 agaagataat tgggaagggg aaactggatt gataacagat gttgtggaca ggtatctctc
   197761 cgagcaagga gacgagattg acagagaagc gtatctctgt ggcagtccgg gaatgataaa
   197821 tgcctgcata cgtgtcttga ttaggcacaa agtactcgaa gaaaaaatat tctacgacaa
   197881 attcggttag tgtggaggtg agaaaatgaa acaaactccg gatttcgaca gaattcaaga
   197941 aaatatgaga gcgggaaata tcaccggtcc tggttttttg ggtgatgatg acaggaatct
   198001 tgttgatata atcagtgaag accagataac cgtcaaagaa cttggcctta caaacgagat
   198061 tatcgcagat aaactggaaa tgcttatgaa ggagggtgaa agaggattcg gttcccctgt
   198121 gaaggtcgac gatcgttttg tagtgattgt ggaggaaagc agaggttaca ttccatgtcc
   198181 atttcgccat ggtcatcttt caaagaaggt aaacgtcaat gtgagaaata tcgctctgaa
   198241 ggaagagatc gattattcgc cgatctccat tcacttaata cgagaacatg gattttttca
   198301 gggcaaaggt tcaccttata gacttgaccc cgcaagagtt gcaaagattc tagagttggt
   198361 ttgactgcag atgggaattt tgaattcagg caagaacggt aagaaatatt cctcgagaaa
   198421 cagagccctg tttacggtct tctttgtggt attgcttatt gcctttgtat cgtattatgc
   198481 ctttggatac aagatggaag ttgtagttcc ttctgaggat gtgaaccttt ccgatttgac
   198541 cttcaatgac gatgttttct ctcttcttgg agaagcgccg tttccaccag accagggtct
   198601 cgcaaacgga gtctctgtgg accgagaaga cggggagagc atacgcgtat tttatccacc
   198661 tgaggataat tcggttgtct gccttcagtt tgaggtgaat tccagaaata tcaatgtgta
   198721 tatttggaag acattttcag ttgattccgc aagaagcgtt tgggacacgt tgttcctcgt
   198781 ggaagcgtct gtgctaacca gggatctagg aagaataaaa aaacctgatt actattacgc
   198841 aaaaatcgtc agatttggag gacgggatca aactctcatc tggcagaaag ggaaatgggt
   198901 cgtgttggtg aagagtccag gatttacgct tgaagaagat gaagagcggc taattctaac
   198961 ggagcttttc gattctagta taagaagtta gtctcatctt cagagtagac ttcgataccg
   199021 ttctttagaa gaagttccgc agtgattccc ataccgtcaa ccattactcc actaaaggtt
   199081 ccgtcataaa tgcccctgca tccacaagag gggcttcttg atttcagtat agctatatta
   199141 actccagata gtttggcgat ttttagcacc tcttccgcac cccgtaaata gttttgagtg
   199201 acatcatttc caaactgatc tagcaccaac ttcgctgccc tccaatcact ggaggttgtg
   199261 acttccgctc ttggacgagg tgtcggaagt cctccaagct gttccgggca taccggtaaa
   199321 aaaacaaacc tgtcaacaag ctctaaaagc tttaaggaca ggttattatc tccacggtaa
   199381 gtgcagttca caccaatcag gcaagcacta acaagtatct ttgtcattta ccaccggcgg
   199441 cagttccatc gtcctctctt ctaattacgt acatcagatc acgtgtggga agcattgata
   199501 atggattcat tgtgttggct cttggatctc ttacttcgaa gtgaacgtga ggccccgtag
   199561 agaccccagt actccctacc cgacctatca gtgatcccgc atcaaccctc tgaccaacat
   199621 aaacgctgat tttgctcata tgaccgtaac gtgtcaagta accgttatcg tgttgaatct
   199681 caaccatgtt tccgtagccg ccatttacac cagcaaaaac aaccgtaccc ccagaacccg
   199741 caaatatcgg cgtcccttcc ggtgcagcga tatcaagtcc tgaatggaaa gagggttgcc
   199801 cggtaacagg gtgggttctc cagccgtagt ctgatgaaat gactccgtat attggccacc
   199861 tgaaaggcga gctttgggga atatattggt atagattgac caacgacata ggaatgaaga
   199921 ttctttgtcc cggattgata acggaatcag gcttaagatt gttggctgca attagttcat
   199981 caacgggaga aaagaaaagt ttcgcgatat agtcaaaggt gtcgcccgac tgaacttcat
   200041 agatcaaacc atccggctgg ggtattcgaa gcgtatcccc ggcctttata ctgttgggat
   200101 ttttgagatt gttgaaatca ataatagttg agatgctaac gtccaaatct cttgaaatag
   200161 tatgaatcga atctcccgct ctaaccacgt atgtaacaag aaaaaacgcg aaactgcgag
   200221 tcatcaacaa cgaagacata aataggaata acagaatgac ccgacggttc attccctgcc
   200281 acctccaaaa agccctaaag aaaagaagac aaccaaaatg attataccaa aaagcagtcc
   200341 aacggcaaaa tgcaaaccaa aaatcgtttg taaaataatg gataaaccta tcagtactga
   200401 gaccgcactt cctaagagta ccacggaagc cgccgtgtcc ttcaatactc gtactctttc
   200461 gtcatagtat ggtcgcaaaa tgtctgcaag cttctctatt agagtattaa ttccttccag
   200521 tagtaatacc ataaacgtac agaaaagcag ccaataataa tcattcttat ctacttggat
   200581 cagcgcacca agagagagaa taacggcccc aatcacaagc tggatcctga agtttctctc
   200641 tcttagaaga tgttttattc cgttagtagc gttccctagg cttctaacaa actttctcat
   200701 tcgaatcccc ggccgacaga atcgattctt tctcttctaa tactgtaaga cttctctatt
   200761 agtgagtaac cgaaatcaat cgcctgggca ttcatttcaa catatttcgc tttaatgtgt
   200821 ttcgtcatgg cctctttaag ggactgagta gtcacgaaac ttcccgctct ggctattagg
   200881 cccaatccga ccatgtttgc tggaagagtg ctgccgaatt ttttttcagt aagctcggtg
   200941 actggtttct gaataatctt tctggtcatt cttactcctt cttttggaat agaactgacc
   201001 atggtggagt cgactatcag tactccgttc acatgtaaga gagggacatg agcgtttaca
   201061 gccgtctgat gcatcgagta aattagttcg aaggaatgag ctttgggata gtcgattggt
   201121 tcactgttaa aaagcacgtc acaatacgag agtccgcccc tcacctgagc cccgtaggat
   201181 tgggtttgaa ctacccaatt tccttgaata attagggctt cggcaagaat tattcccatc
   201241 aaaacgttgc cctggccccc aatacccgaa atccttactg aatgaggttg tgtgaacgct
   201301 tgcttcatcc attgtcacct ctttctgcct tcatcaattc ctcgatcttt tttctgtagc
   201361 tatcgctgta tgtatcagca ttcttgttta cgaattcacc ggccacgatt ttatcggcca
   201421 attcctctgg agacattgtc ctggccttgt taatttgcac tgtgttcttc ttgaaatagg
   201481 caagcatgtc ttcaggttgt gacatggcgt tgtatctacc gtagtatgtg tgacagttgg
   201541 tcattatttc tactactgcc atacctttgt gatcgagggc cctctgaatg aactgaacca
   201601 tctgcgcata gtgaaagacg gttgatcttg cgacgtatgt tgctcccgag gcaagtgcca
   201661 gattgacaat gtcaaatgag ttctctacat tgccgtacgg agaagtcgat gcgattttct
   201721 catgaggtgt ggttggagag tactgccccc cggtcattcc atatatattg ttgttgaata
   201781 ttatcaccgt tatatccata ttccgtctgc aagcatgaat gaagtgattt ccgccaattg
   201841 cgagcatatc tccgtcccca cccatcacaa caactttaaa ctcaggtctg gcaagcttga
   201901 cccccgttgc aaacgctatt gctctaccgt gaagagtatg catagtgttg aagttaagat
   201961 agcccgttac ccttgaagaa caaccaattc ccgagacaac agagactctg tctggatcga
   202021 gagcattttt gtcgacagca tctatgaatg ctttcataat gacaccgttc ccacaaccgg
   202081 gacaccaaac gtgaggcatt ctatcctgcc tcaaatactt agttagcctt gcaatagcca
   202141 tgatttcctc ccattcattt cgtaaattgt gtcatcgtga catttgtttt ttcaaacatg
   202201 aactcggtca atgggtcagg atatccctca gaaaaaacga ccctctttat tcccgcattt
   202261 attataagtc tggcgcaaat cgaacacgga cgatgagtta cataaatagt tgatccattg
   202321 gtcgctattc caaatttcgc tgcttgtgca atcgcgtttt gttcggcatg ggccgcatag
   202381 caaatttcct ggttttgacc ggaagggatt ccaagagcgt ctcgaatgca ctcggtttca
   202441 tcgcagtgtg gaaatcccga aggtggctgg ttataacccg tagacagtat cctgttctcc
   202501 ctaacaatca aagctccgac cttcctgtgg tgacagctgg acctttcccg aacctggtag
   202561 gcaagtctca tgaaatatga atcccagctc tccgctttac tttccggttt gtgtgtggag
   202621 aaacccttaa gaaaatccga cagatcttct gcagacatca catcattccc ttcagaagtt
   202681 caatatgatt attctcttct ttaataatcc tcttaagggc atctgctgtg gtaaatcttg
   202741 cgtttttttg aatgctttcg tagaatgcta tgcttttctt ctcagcttca attgcaagag
   202801 aaattatctt ctgataatcg gaattgctca tagaatcatg aagacttctg gagaaaacac
   202861 taccatttgc caggtcttca acgtatgccc ttgagtcatc ccagtttatg agttcgaggg
   202921 actcctcttc gaatttcctt agcagaaact tgaatgtttc cgcatgtcca tcttcttgct
   202981 tcgaaagata tctaaagagt gaacgcttac tttcatcatc aacgttttct gcaagttccc
   203041 tgtaaaagct taccccggct tcttcgactt tcactgccaa ctcgagtatt tcatgtggag
   203101 aaagcaatca ccatctcctc cttgatatcg ataagcttct ccagaacgct tctagaaaag
   203161 tccattccat tatcgttcaa tcttattcga ctgttatctg gcgaaaggta gtcaccaaga
   203221 tgagctctaa gcttggagac caaagtatta ttcaactcat cgggaagttc cagctttgat
   203281 aaatcaacgc cttccaccag tcttagtccc ataaaaagcg tttcaaaaac ctcttgaatg
   203341 actgagttcg ccctcaattc aagaaccggt ctccttcgaa gattaacgag atatcgatac
   203401 tcaaggagat ccgaagtatt cacgtacctc tttctatcga agtggctacc ggctgaaacg
   203461 ccaaagccca tatattgctc gtttcgccaa tacttgagat tatgaagaca ctcacgacgt
   203521 cctctctccc atgatgagat ctcatatcta ctgaacccca ttttgcttaa agaatcaaga
   203581 ataatatcgt gaaggccttc tactatttct tgtctcggta aatcaatgat atcatcatgg
   203641 acaagccgca tcaatttagt ttcgtggtca gaatcaaaaa catagtacga tacatgatcc
   203701 gggtttatgg cgctaatcat ttcaaggttt ccggcaacgt tgctttcgtt ttctccaggt
   203761 aaccccagaa tgaaatcgag atttaggttt tcaaagatat ctctgctggc catcagtctg
   203821 tctctgagat tcttagggac gggtctatcg catcgttcga ggatctccct ttccgatgat
   203881 tgtataccga tcgagagcct gttgaatcca atatccctcc actgcttaag gttttttata
   203941 ttgagttccc aggggttggc ctctattgta acctccactg gcgcggcatc aaatgagcag
   204001 gaaatgaacg acatcaaatc cctaagaagc tcgacaggat ataaggaagg cgaacctcct
   204061 ccaaagtaaa gagtctcaac cctctctgaa gctggataca tttctacttc cttcttcagc
   204121 aagtcgttgt attgtcgtac gagatcacca tcatactcaa ccgtgcaaaa atcgcagtat
   204181 gagcatctcc tcgcgcaaaa aggaatatgg atatagagtc ccatgcccat cagaaaataa
   204241 gcctcatgaa gatcccgttg agattgaagt ttgttaccga gattccagca aagattgaat
   204301 catcactccc aagtttcgag ataagtaact gagcgtcaat tccacgcatg ctcaccatta
   204361 ctgtaggaga aagcataagc ctcaaatcgc ctgcgggaac aataaagctg cctgcgaagg
   204421 ccagtccggg atcgaaggac aagtagttca ttcctatacc tataccatat ccaacctcgc
   204481 ctagcgagtc gtttgtttta aggtcaacag ttgcgacaac attgctttcc atgtttctaa
   204541 ttctaagact tcgctcatat tcttttgcat aaaggtatag accaatatcg ttgaatgaaa
   204601 aggaatcctc tccgtgaaga aagacactta atcctccgtt aagctgacct aaagggagtc
   204661 gtacccagtt tcttacaaga tacttcatct tgctaaccgt cgaaagttcc agttcaagag
   204721 cggtgtgaga aaagatagga atctgattag tgttagacac atacctgact cccataaata
   204781 gaccttcgtg actggccaac aatgatcctc tgaaaacgcc gtcctggatt ttcaacttca
   204841 aaaatccggt agagtactcc tcccaactgt caacatatgt gacaccaacg taaggaattc
   204901 ccgtagccaa tgctagagtc aaatagatgt cctcgtaagc cctgatatct gctgaaacac
   204961 tccatcctgc gttgttgagc tttaactggg tttcggaaag aagaagaatc ccaaaaagca
   205021 atacaagtga cagacataaa actctcttca acctttgagc atcctccttg cgatttcatt
   205081 gactttcgat ggatttgcct tacctcttgt tgccttcatt acagctccta cgaagtaacc
   205141 gagaacacct tcctttccgt ccctgaactg ctgcacagcg acgggattgt taaccatagc
   205201 cttctgaagg gcatcctcga ttaccttatc atcatctatt tgttcaagcc ccttttctct
   205261 tactatctct ctgggcgact tcttttcttc aacaactgtc gggaagatat ctttggcgat
   205321 cttgttggag atctttcctt catctattaa atcaaaaagc tctttgaaat gttccggact
   205381 taccttgacc tcttcgatgg taagtccctt gtcattcatg aatcgcatta cctctcccat
   205441 tatccagttc gaactctctt tcggctttcc agtgacctga gcgacttctt cgaagtatga
   205501 agcgatgtcc ccgtctgaag agagtacgct tgcatcgtac ctcgggagcg agtattgctc
   205561 aacaaatctg tcgatctttt gccatggtag ctcgggaaga ctttctttga tcctttcgat
   205621 gacttcatca ctgactacca gaactggaag atccggttca gggaagtacc gataatcgtt
   205681 ttcttcttcc ttcgatctca ttgaaaaagt ctctttggac gaaaagctcc aggaccttgt
   205741 ttcttttgcg acgtcctctc cagataagag cgcttttgag attctctctt gctcgaattc
   205801 aagtgccttt tcaacgaact tgaaggaatt gatattcttt acctctactc tattagagct
   205861 tcgtcctgtc tcatcgacca ttgagatatt cgcatcacag cgtaatgctc ccttctccat
   205921 atctccagag catacctcca gggccctcag cgtgtctctt agaagctcca taaagatacg
   205981 agcctcggag ggactacgaa ggtcgggctc ggttacaatt tcaattaaag ggacaccgca
   206041 tctgttgaga tctacgaggc ttcctgacga tccggttata gaatccgttc cctgatgcac
   206101 cattttgcct gcgtcttctt cgatatgtat cctgcgaatt ctcacttttc ttttctcttc
   206161 tccgttctcc agatagagat aaccgttttc tgcaatcgga aagaagtact gcgttatctg
   206221 gtaacctttt ggtaggtcag gatagaagta attctttctg tcaaagacag aacgtttgtt
   206281 tatggtgcag ttcatagcga ttcccgccaa aactgcgaac tctactactt tcttgttaag
   206341 caccggcagt gtgcctggct gcccagtgca cactgggcag attgcagtgt taggttcgag
   206401 atcgaaaaca tcggctctac agctgcaaaa ggctttcgtt tctgttttca gttgagcatg
   206461 aatctccagt ccgataacag ttcttagcat tactctctcc acctttctgg tatgatcgca
   206521 agcccattac taaaagaggg agacaaatcc tcaattcctt tggcggcatc aagtatggag
   206581 acatcactga atctcttgcc cgtcatcaaa acacctaccg gcattccatt gacaaaacct
   206641 gcaggaactg agatcgaagg cagtccagcg acattcgccg gaatcgtata gatgtccatc
   206701 aagtaatacg ccagtgggtc agttatttcg cctatcctag gagcgactaa aggagaagaa
   206761 ggattcaaga tgaaatcata ctcatctagc actttgctta gttcaccggc cattactcgt
   206821 ctagtcttca aagcctttct atagtaagca tcgtaataag tcgcactcaa ggtgaacgta
   206881 ccaagtagaa ttcttctctt aacttccttc ccaaaacccc tatctctgct cttgcttgtc
   206941 agaccttcat aatcctctga ctgctgcctc ggtccaaacc ttattccatc ataacgagag
   207001 aggtttgaac tggcctctgc gggagcaatc aaataatatg tggcaacggc gtattcgaca
   207061 gaaggtattg atattacttc aacatcggca ccagacctct ctatgtctct tgttatggaa
   207121 agaaatcttt ctttcacctc ttcatcaaga ccgggatagc taagcatttc cgaagggata
   207181 gcgaacttca ggcccttcaa gtacccacga agcgaaacat caaaggaaag agagtgtctt
   207241 attgtattgg agtcacaaga atcatggcca gagatcatcg acatcacgtc tgcggcgtct
   207301 tgcgaacatc ttgtcattgg gcctatctga tcaagagaag aggcaaatgc aaccagacca
   207361 tatctcgaaa caagtccata agtaggttta aagccgacga ttccacacat ggaggctggt
   207421 tgccggactg aaccgcccgt atcgcttcca agcgcaaaag gtgtgagacc tgccgcaact
   207481 gctgcggcac ttcctccact actaccaccc ggtatacgat tcagatccca gggatttctc
   207541 gacggtccaa acgctgagtt ctcagttgat gaccccattg cgaactcatc catgttcgtc
   207601 ttgcccagaa gctgtccacc tttggaattc aatttctgag ttactgttgc gtcgtatggt
   207661 gaatcgtagt tttcgagtat tctgctcgca caagtcgtcc ttgttcctac cgaaagaata
   207721 ttatccttta tggcgtaggg aaagccctgt ctttcttctg aaagggttct tacctgaagg
   207781 aatgcattga gttcattgtt gtatttgtca attctctctt tgtagaactt gtaagtgtct
   207841 ccgagagaaa tgatttccga gagcctcaga tttagcgggt tctttgtcac gaaagccacc
   207901 tccagatatt catgacaatt ctaacatttt cagggcactt ctaacagaag agagcaactc
   207961 gaatatactg aaggccggaa gcataatctt attacatcta ccactagtga gaagttatct
   208021 gctatgtaca cgctttaacg aaactctgtt agttgcgctc gaaaagggaa taacggttca
   208081 cggttttcct gacaagagcg atgcaatttt tcggcgcatt ttccgtagac gactatctct
   208141 ggtaatggct atggtaagca taagaatctt gcttccattt ttcgccattc cattagcgaa
   208201 tctaactcct cggcaaggat cttacttggt cgaaattatc gaaaagagat gctgaaacaa
   208261 gttcagatga ccgaatcaag agggttctcg catccggcat ctaggggttt gtgaacagag
   208321 gcggttgacc cacgaggacc ttcagggcta tcgaggttgt tatcaagtat tactcatact
   208381 aattccttac tttgtcatac tgtggagcct cccctgaaat tttcttgttc agggtcatca
   208441 gcaattcagt tcgggatctg ggtcattaaa aatcgttttg gaagcggaaa taaaaagaag
   208501 ctgattctcc gttagcagaa aaggatccat ggtacagagt ctgtagagac tacttacaaa
   208561 tgctctagca acttgcagtt gttctttcaa gagaacggag aaccgttatt agttaactga
   208621 ttgtcctgtt ctaagtgcta cttgtcggta cagtgatctc tcaattttat tccctcttct
   208681 gagctttcca catcctggca ctctctaatg aaggtcgtta gctatgtatc cactctcagg
   208741 caggcaaaca tcgtaaagcc agttccacga ctgagcacac tcggttgaat ccatttcgaa
   208801 gtgtgcgacc gcatatcccc ctgcaggaac aatcatcttt ccaatatcac cgtctactct
   208861 tgtgtcttga ggaactgtaa tgcacatgct cagtctcagc ttttcctctt cagtcacttc
   208921 cggattatcg tggtaaatga tgagagaact tgcgctcttc ttttccagaa atcctcgagg
   208981 acctgcccag gaacagagtt tgttgaacaa ggtttcgaat agtttctcat caccggcaca
   209041 aggacctatg tagcggacat aagcaaccgt actctcctca aaattcacgg tatctactct
   209101 ctcggctttt tcccatattc atcctcctaa aaaaactgtc gatgctacga ttgtggaggc
   209161 gggaaagaat gttgggtgat ctacatcgcc ttcgaaaata cctaaattgc tttcagtcac
   209221 tccgctattg atcttattgg gtttctcatt tttccactgg agtggattca ttccaaaacg
   209281 cttcttgata cttctgctga gcgatgatcc gctggcgaaa ccgcactgat ctgcgacctg
   209341 agcaactgta ctattgggat cgtttgtaaa gagtgtaaca gcttttccaa gccttgttct
   209401 ccatatgaac tggaagaggg tgtttccagc ggaggcatgg aaaattctgt ggaagtgata
   209461 tttcgagaat cccgctacat ctgacagctc atcaagcgtg agtgactctg acagttaata
   209521 tcctatgtag ttcattatct cgtttattct tgatatgcag acgccatgta aaaggcgctc
   209581 agggatgaac tgattcatat cttatctttc cttttggaat ggaatgtaag tcgagatgcc
   209641 ttgcgctctg cttcaagcat gaagtggatg ctatcattag gtaaaggtgc agcttcaagt
   209701 gaagtatctc ttttttctgg tgaataggat ttcttagcgt gaaccttcga caaaccaaat
   209761 acctcctccc gattcaaggc cactaatacc aactggttct tttactggac cttcgagcaa
   209821 ccaaattacc cttgtccaat gcacaaacat cttaaatcgc ccatagtata acctcaatgt
   209881 taattagtca aatatatcta gttctgcaga aagaatcaaa cattaaaact ggccaaatta
   209941 aaaactaaaa ttgatccttc tcaatacaat gaacaaataa tatacctgaa aactggacaa
   210001 taattcactt cttccaattg cagttgcttg attccataat atattcagta tttttcttta
   210061 tatcagagca aataattata gtaattccaa taataattat atttatctaa gattttttta
   210121 ttttacctct tgacttttg
//
LOCUS       DAOQYI010000003       117801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_133_length_117801_cov_75.176923, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000003 DAOQYI010000000
VERSION     DAOQYI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 117801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 117801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..117801
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133_length_117801_cov_75.176923"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKL93_02200"
     CDS             complement(<1..309)
                     /locus_tag="PKL93_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="giguanylate cyclase"
                     /protein_id="HNQ70110.1"
                     /translation="MRAAKRGIDEGLYIARALLEGNVEACAIVSSEGKIVVANSTWER
                     LAAACFDELSFLSEEFDMKRFSDMLDEVCALRLKEFLSGKTKQVSFALNDFENSKGLP
                     F"
     gene            405..998
                     /locus_tag="PKL93_02205"
     CDS             405..998
                     /locus_tag="PKL93_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ70111.1"
                     /translation="MKKEKIEYRQSESSDLQFVAHLVFNTDPSHFKRALGRKALAGIS
                     RLLIMGSAINLEFITVCEIGNRPIAIYAFYTHEEMNRLSHLHVDMINFSFSIFGFRLL
                     HEFRTLQRSMEMGIFPEQKYLGLISVHEDFRNKGYGRRILNHLLEMDAEIVLDVSRWN
                     RKALSLYSECGFEFETNLPSNYPTKDSIRMKSTRDHH"
     gene            complement(1041..1835)
                     /locus_tag="PKL93_02210"
     CDS             complement(1041..1835)
                     /locus_tag="PKL93_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNQ70112.1"
                     /translation="MKVFISADIEGTAGIADWNEAKKGNQDYEYFKKQMTAEVKAAVQ
                     GALEAGASEITVRDAHGSARNIDPSLLPEGVRVLRGWARDPFMMMQGIDSSFGAVVFT
                     GYHSPSGKALNPLSHTMTGDIYYLKINNKLISEFELNAMSASYHGVPVVFISGDEGIV
                     QIAKQNVPGIQTVSTNIGSGASVDSIHPAKAVKLIEEGVYNAVKNIGVQPVSIPAEFE
                     VEIAYIEHTLAHKLSFFPGVTKKDDRVLTFRDQDYYEVLRKLLFLL"
     gene            complement(2046..2708)
                     /locus_tag="PKL93_02215"
     CDS             complement(2046..2708)
                     /locus_tag="PKL93_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HNQ70113.1"
                     /translation="MTLNDWTLALKETGYEFARFLKKRGLCCSVTVISEYCVELSNSV
                     VLRLYTNRKGNKRMVLPSFLSEEKSEWLLFRWEEFNGRNYDGVHLFVDGSFMDNKVGY
                     GVLVITENQIEKRINGCLRENLEMKNVTGEIEGVIRALEYCMRKGYRKATIHYDYMGL
                     RSWAEGEWKAKYKLTRKYVERLKQFSSQIDIEWVKARSHIGETFNEIADKLAKKAVED
                     CG"
     gene            complement(2705..3475)
                     /locus_tag="PKL93_02220"
     CDS             complement(2705..3475)
                     /locus_tag="PKL93_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNQ70114.1"
                     /translation="MWDYIVLRVSLFFIFGLIFGSFSNALIFRIPSKEYSMTRPRRSI
                     CPKCKHQLSWKDNIPLISYIFLGGKCRYCGEPIAARYPVVELLTALLFSLNSVVFSLF
                     GAISMSVLTMGLIVSSFIDLEHYMIPDFGVILVAAGGFAWSTVEGRFPANLLHALLVT
                     GAMVAFFFIANLVRKDSFGFGDVELLAALSLASGIIGSLYTIMVASFAALIVYAINAA
                     AKGRKFDRRAQLPFGPFIAVGGYTTILFLDFIEALYRV"
     gene            complement(3460..4530)
                     /locus_tag="PKL93_02225"
     CDS             complement(3460..4530)
                     /locus_tag="PKL93_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ70115.1"
                     /translation="MEIIRLKKDKKSFDITDTLPFAIAILDEVYDGDINVAIHDLKST
                     KIGKDLLTIGGLAETFDNVVTPENYAPIEYEEFNDYLKETGLSVASFTRGTFSGFQDK
                     ILSIADRGDYESAQKFLDLLMDANVDKATVCELKGSIFLESGDEEEGIKWLQNALKID
                     CSLVTAYSFLGQTFYNRGDFDSAADCWQREIAASPDHLVTYFMLTDAYINAGRMDEAL
                     NVLKDLSKRDPDSILTKVEMAELYEKMGNIEMRRIVEDEVLRTKPVYINDVEPWAKIQ
                     FKHSNYDVVEEVATNFLKENPDRPELKMLLVVSHMKKGQCDEAKRLLKEFENEQVWYF
                     YGKKELFDEYLTEEERKQCGIT"
     gene            complement(4777..6990)
                     /gene="rnr"
                     /locus_tag="PKL93_02230"
     CDS             complement(4777..6990)
                     /gene="rnr"
                     /locus_tag="PKL93_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNQ70116.1"
                     /translation="MKLSLKALKEEMTSKDYSPTTMRGLIVKLGLTDREDRALLKKYL
                     EELTDSGEIIKDLKGRYIVPGEKTIVGTIEFARRGTGAYISVEDEEDVFVAPEDSGFS
                     IHGDTVLVEITGRYREIRKGKVIKVISRNKKKVVGTFKTRGIMAFVVADDMRIKTDFY
                     IPPEGFNGAKPDEKVLIKITRYPSPGRNPEGVVERVIGDVFSPEADLMTVIYRQDLPE
                     PGYFPEQVRKQAARVAKSIDLQEVRKREDLRNELVFTIDGDDAKDFDDAISIRKSREG
                     NYILGVHIADVSHYVQEGTQLDLEAYSRGTSVYLLDTVIPMLPFELSNDICSLKPEED
                     RLTLSLLLEIDPVGRVVSSSVKNSVINSKKRLTYSEVNDFLNGKASSDISEKLRPVSE
                     SLKLSHELAQKIRDIRKKRGSVMDISSREVKIIFDEKGRVTDILPQSRGISEWIIEEF
                     MILANETVAEIFNNQGLPFVYRIHDKPDPESMMQLKNYLEALGLKVKIPRNVHPKALQ
                     QILEKSKDHPLHSSIERVLVRGMKRAVYSDRNIGHFGLASEAYTHFTSPIRRYPDLVV
                     HRLLKDLIKNAGADLKNHSKNLEESLSKIASWASKRERIANEAEWDLVDMKKVEYISN
                     HVGDSFEGVITGVTKFGIFVELTEKMISGLVHISTLDDYYDFIEDQNILVGRRSKRVF
                     RIGDQLEVKVVRADKTTMEIDFIIVNDNGKTTRRSKRSSPDKSKGKNSRSHKKSR"
     gene            7092..8114
                     /locus_tag="PKL93_02235"
     CDS             7092..8114
                     /locus_tag="PKL93_02235"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNQ70117.1"
                     /translation="MRIGILTGGGDCPGLNAVIRGIVKSAPENVEIIGLMHGWKGLVV
                     GESMKLTDNDVEGIHILGGTILGTSRTNPFKSEDMKIKMEENIKKLGLDVIIGIGGDD
                     TLTVAARLSSMGYKAIGVPKTIDNDVANTDYTFGYQTAVNVGADAIDRLHSTAKSHGR
                     IIIVELMGREAGWVTLEAGMAAGAHLILIPEYPMTIDEILTYVKKRMDRFGYMIIAVA
                     EGFKPTELENVVGDESKIDAFGHIKLGGIAHYMANIISERTGYDTRSVVLGHLLRGGT
                     PTAFDRILGTRYGVHAMELVMKGDFGRMVALKGNDIISVPLEYGIATKKLVPFEYYKL
                     SQIFFD"
     gene            8124..8639
                     /gene="amrA"
                     /locus_tag="PKL93_02240"
     CDS             8124..8639
                     /gene="amrA"
                     /locus_tag="PKL93_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNQ70118.1"
                     /translation="MYYSEPVRIARCAMEEFIRHGVLIGTPDWVSEEFLSRRAGCFVS
                     LHTSNGSLRGCIGTIYPLQENVALEIIQNAISASTKDPRFPPVEASELADIDVSVDIL
                     SPPERTTLSGLDPKKYGVIVSLGYRKGVLLPDLEGVDTIQKQLSIALRKAGIRESEDY
                     SIFRFSVERYR"
     gene            8660..9655
                     /gene="amrS"
                     /locus_tag="PKL93_02245"
     CDS             8660..9655
                     /gene="amrS"
                     /locus_tag="PKL93_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNQ70119.1"
                     /translation="MKPAVYFDEYGEEKVLQCLLCPLHCIIKPDQVGFCGVRKNVDGM
                     LYSLNYGQLTSIAIDPIEKKPLYHLFPGEKILSVGSWGCNLRCNFCQNWEISKQRPKT
                     VMRVMPQQLIQIALERESRGIAFTYNEPIVSFEFILDTSRAAAKEGIYSILVTNGVID
                     EEPMDLLSQSVRGMNIDLKGWNDEFYVKDIGTEKRVVLRSIETARKAGTHIELTTLII
                     PGKNDSPEEMREEAKWISGLSKDIPLHINRYFPNYKSTIPPTSPDSLIQLAEVAKEYL
                     RYVYVGNIDLPGLSDTTCPYCGNLLVERRGMRSSVVGIDEKGRCNNCQKEIPIVF"
     gene            9616..10665
                     /locus_tag="PKL93_02250"
     CDS             9616..10665
                     /locus_tag="PKL93_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNQ70120.1"
                     /translation="MQQLPKRNPNSLLRNRIFLYGLLFVSAIILLTIVFFRQKPLEYY
                     DKTEYHLGTYVTIRVSSSTMSPVRLAEAAMTEIKRIDDKFGSRNNGVIAELNRTGELH
                     DIDKETSFLIRSALEIARNTSGAFDPTLYSLTRLWGFDDASSKKRIPSDKEIEDALGS
                     TGFSRVTYDEELGYVSLVDGAMIDLGGIAKGYAVDMAIAKIKSLDPEATGFIDAGGDI
                     GVIGPKYGNRPWIVGVRNPRGESVQDVLEYIYLYDGAVATSGDYERFFIENNVRYHHI
                     FDPETGFPSRSGVISATVIGKSTMLADAYATAAFVIGMEPGVTFLPRFGALVLLVMED
                     MSIFRSPGFEVYQEK"
     gene            10662..11012
                     /locus_tag="PKL93_02255"
     CDS             10662..11012
                     /locus_tag="PKL93_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNQ70121.1"
                     /translation="MKFTTRNDVLIVIVAFALVILFAFPRGNGSSFAEVYCNGRLVRT
                     IDLSSDSEIFLDVGMVVKVQGGKISVIDSDCPEKLCVAQGAVNMPNIPIVCVPNRTLI
                     KIVSDRSEVDAITQ"
     gene            11161..11673
                     /locus_tag="PKL93_02260"
     CDS             11161..11673
                     /locus_tag="PKL93_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNQ70122.1"
                     /translation="MMEAKRVTTLSMLMAIGSVLYLLESLIPFPLPIPGGRWGFSNLV
                     LIIALAGLPFGDILTLSIGKSIIGSLLAGRLATPGFLMGVAGAVSSAATMWLMNKTGK
                     FGLFGISVAGAAISNFSQLMVASSLIIKNIEIIFLYPYMLVLGSISASINAYVAFEVI
                     RRMGDSLWQD"
     gene            11661..12224
                     /locus_tag="PKL93_02265"
     CDS             11661..12224
                     /locus_tag="PKL93_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNQ70123.1"
                     /translation="MARLILGSSSPRRRELLHLLRVSFEVIPPKGVEENLSERFCEKE
                     LCELSHLKAKNVMLRNPFSTVIAADTVVVLDGLVLGKPSSVEEAYEMLLVLSGRTHSV
                     FTSVSVMYEESEFSFVEKTDVTFREVPSEVLKEYAESGLSLDKAGAYGIQDYGALFVK
                     SIAGDFYNVMGLPIGRLWHELHARGVI"
     gene            12241..12906
                     /gene="radC"
                     /locus_tag="PKL93_02270"
     CDS             12241..12906
                     /gene="radC"
                     /locus_tag="PKL93_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNQ70124.1"
                     /translation="MPREKLLDKGAGALSNSELIAILLRTGIKGKDVLQLSEELLGYY
                     GSLTALLSAEVNELLNTKGLGKAKAVCLKAALELGQRIFREMTEKTKVRLDEPEGVYH
                     LCHDMAFMDRETVRVISLDSKLNYRGLNTVSVGLLDSSLLHPREVYKPAISRMAAAII
                     LVHNHPSGDPSPSKEDEGVTSKIREAGETLGIKMLDHVIIGSGTFYSFAAGRTFKAEV
                     AEK"
     gene            12903..13451
                     /locus_tag="PKL93_02275"
     CDS             12903..13451
                     /locus_tag="PKL93_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNQ70125.1"
                     /translation="MISRDHAMQLIMNNIQSKNLIKHVLATEAVMRALAERLGENEDV
                     WAMAGLLHDLDYEFTKDNFDQHGLKTVSMLQNEDLPDEVNDAILAHCEKKDRVKLIEK
                     AIYAADPVTGFIVAAVLIRKGSKLSDIDVDFLKNRFKEKSFARGASREQMASCSEFGM
                     SLEDFLSLSLKAMQEISVDLGL"
     gene            13486..14235
                     /gene="deoC"
                     /locus_tag="PKL93_02280"
     CDS             13486..14235
                     /gene="deoC"
                     /locus_tag="PKL93_02280"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNQ70126.1"
                     /translation="MLKAIIEREVRRYKEEYSPIKRELPENLNIAKFIDHTLLKADAT
                     PKMVEKLCEEAIRFEFWSVCVNTGYLPLVNRSLSGSTIRKTVVIGFPLGQMSREVKGF
                     ETSWACDNGADEFDMVINVGLLKSGEYESVFDDIAKVVESACGKTVKVIIETSLLTEE
                     EKIVACVISREAGAAFVKTSTGFSGGGATVDDVSLMKFVVGDACKVKASGGVKSREEA
                     LAMISAGASRIGTSSGINIVSEGSKENWGGY"
     gene            14237..14866
                     /locus_tag="PKL93_02285"
     CDS             14237..14866
                     /locus_tag="PKL93_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNQ70127.1"
                     /translation="MDFCTLSLKDFLKKVAEKSATPGGGAVGAVVAALAASLGSMVAN
                     LTIGKKGYEDVEGHMESCLEVFESESNYLCDLMNRDIQAFDQVLSAYKLPKATDDEKN
                     AREMKIQQALKTAIEVPFDLARRCKNIIVNVERLAKWGNSSVLSDAESAAHLLLAVHR
                     IAKANVMINMKSLKDPDYGAWINEEMKQLEKQIDSSYNKIIEIIGKRNG"
     gene            14859..16004
                     /gene="tgt"
                     /locus_tag="PKL93_02290"
     CDS             14859..16004
                     /gene="tgt"
                     /locus_tag="PKL93_02290"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNQ70128.1"
                     /translation="MVELKLKAKSTESKARTGVIVTPHGEFETPVFMPVGTNGTVKGL
                     WQDQLEELDARIILGNAFHLFLRPGLNVLRNFGGLHKFMSWKHSILTDSGGFQVFSLK
                     EKKITDDGVKFRSPLDGRALFFTPELSSEIQEAIGSDIAMAFDECVEPSANKDYVRQS
                     VKRTTIWAERFLLSHKGQSMQSLFGIVQGGFFSDLREESAARITSLDFEGFALGGLSV
                     GEDFQTTERILSGSVNLLPEERPRYLMGIGSPELIMAAVENGIDMFDCVLPTRLGRHG
                     AALTSNGRINLKSSTNKEDKRPVDPDCSCKVCSTYSRAYIHHLFIRDEILGKILLSYH
                     NISFLMQFVKRIRQAILDDRLAEFKEHCIETGLIQTENLKTSEERMG"
     gene            16009..16836
                     /locus_tag="PKL93_02295"
     CDS             16009..16836
                     /locus_tag="PKL93_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70129.1"
                     /translation="MKKLLILIVVFFCVVTVAMNPFQLQTGGNGILAVEYDSGFGSPS
                     DLYGITNSAFSTHFVELTTVASPSSNQRALYLHLFEDSDDGMAGMLQYYMDTENASNV
                     RSLSYTISGALGPFTAYGADFELNMTTATATTYGVSVRGGITGKAYELIEYIVSFSSD
                     LWNSGSSVSSVDLLAGIRFVPDPFMIGLELGTREGMAIKYLGLSTQYTYNNLFSARAG
                     VSMNADLLSNIDFIVGGGMEVRVGDMLITAGIGTNLTNKIESFSFSRNWSVGLLGRW"
     gene            16997..18502
                     /gene="guaA"
                     /locus_tag="PKL93_02300"
     CDS             16997..18502
                     /gene="guaA"
                     /locus_tag="PKL93_02300"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNQ70130.1"
                     /translation="MDRIAVIDYGSQYTLLLARRIREMGVFCTVASPEEFKVESEIKG
                     VVLSGGPQSVYEEGALGFPKQLEGFPVPIMGICYGMHLLAKKLGGEVRSGALGEYGLT
                     NVSMDNERFRDIPEEIVTWMSHGDEVTSLPAECKVIARSKNGITAGFSNGRDIAFQFH
                     PEVNHTQFGTNLIEDFVFNVCNASRNWKLSAFAERKVEEIKRIVGSSKVVGGLSGGVD
                     STVAAVLTSKAVGDAFTGIFVNHGLMRKNEDIEVCEALRSLGIKLINVDASEEFFREL
                     KGIDNPEEKRKIIGKTFIKVFEREANKLNASFLLQGTIYSDVIESGAASKNSAKIKSH
                     HNVGGLPDKMNLKLLEPIRELFKDEVRNLGYSLGIEPSLLTRQPFPGPGLGVRIIGEV
                     DSEKARILKEVDSIFMDVLRKSGESEKIWQSFAVLLPVFSVGVKGDKRSYGYVVALRA
                     VNSSEGMTASWHELPHEILREASSRITSGVREVGRVVFDITDKPPATIEWE"
     gene            complement(18680..18754)
                     /locus_tag="PKL93_02305"
     tRNA            complement(18680..18754)
                     /locus_tag="PKL93_02305"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18720..18722),aa:Gly,seq:gcc)
     gene            complement(18774..18858)
                     /locus_tag="PKL93_02310"
     tRNA            complement(18774..18858)
                     /locus_tag="PKL93_02310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18822..18824),aa:Leu,seq:tag)
     gene            complement(18859..18934)
                     /locus_tag="PKL93_02315"
     tRNA            complement(18859..18934)
                     /locus_tag="PKL93_02315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18899..18901),aa:Lys,seq:ttt)
     gene            19108..19893
                     /locus_tag="PKL93_02320"
     CDS             19108..19893
                     /locus_tag="PKL93_02320"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNQ70131.1"
                     /translation="MVLLADIGNTTTVLGLTGEKSIEEVWRLPSSRIETEDELFVILD
                     GLLRIKGYSVGEIDGLCVASVVPRLNRTINYFTRKYLPKPAVFLRPDRFFPLGLNTDN
                     PAEIGADRLADVLGAKECYGNNVIVVDAGTAITIDIVKDGNFVGGAILPGPGTAMSAL
                     FSHTAKLPEVELFFEDHYFGTNTEDNLRIGIVNGTYFAIQGIIDNIIEKFEEKPTVVG
                     TGGNIDLFLIEGGLIEIADPNLTLKGLSFYYNRVKPFEEDTTC"
     gene            19871..21214
                     /locus_tag="PKL93_02325"
     CDS             19871..21214
                     /locus_tag="PKL93_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ70132.1"
                     /translation="MKKILLVNPWIEDVSAYDYWLKPLGLLYISSLLKHIGIQSILID
                     CLDRYDDELITFSPKFGEKYYGTGKFLESEIPKPQSIASIPRRFKRFGFPEEVLRKKI
                     RQVKNVDCVFVTSMMTYWHYGVHDTIRIIKDEMPSVPVILGGVYATLLPEHARRYSGA
                     DKVCPGTGTEPLKMALGFLGINETLDFDWFDDLDPDYSVYKSARYAVLISSLGCPFKC
                     TYCASSLLWNSFVRRDPVKSARFVKHLTASKGLSDIVFFDDAILVGSGFKRLMEEFER
                     LRIKARFHLPNGVHARFIDRETADLMYMNNFKTIRIGLETSDPVMQNSTGGKVNLDDI
                     FVAIENLSSAGFTSREISAYIMVNLPGQSEEDVLASLRICEELRISPSLNEFTPIPGT
                     VQWKELTRGGVFDEDIDPLLLDNSILPYWWKEGFSPDAVQRLKEKAWSLRRRLND"
     gene            21207..21737
                     /gene="folK"
                     /locus_tag="PKL93_02330"
     CDS             21207..21737
                     /gene="folK"
                     /locus_tag="PKL93_02330"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNQ70133.1"
                     /translation="MTKDLFLGFGSNMGERLNLIIKAFTMLIEREITLQSVSSLYLTE
                     PLGNIEQNEFLNCVGKFEFKGDPHFLLEEIKVIEKSIGRVERFRWGPREIDIDILLFG
                     EMILTTEKLSIPHKDIIRRKFVLVPLLEIDEELRDPRDNSLFSDHLESLGKEGWPELF
                     LEARLFEKLIELEVKR"
     gene            21734..22243
                     /locus_tag="PKL93_02335"
     CDS             21734..22243
                     /locus_tag="PKL93_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70134.1"
                     /translation="MKIRNFSIIVIVVLITTLFSSCSASKSELVPGKFAGFALSDYMD
                     ISTSVSVGGMILGIELNEDTLLLHKEFAKGTKEFNVYVAKFENASRATGFWYDFVREI
                     SNEIRSVISAIPWIYGEFSGEVGESFMKSWFSGRWLYIFLGDNSEEVDSAVRAFKEYE
                     KSLVRSVES"
     gene            22233..23111
                     /locus_tag="PKL93_02340"
                     /pseudo
     CDS             22233..23111
                     /locus_tag="PKL93_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            23143..23470
                     /gene="rnpB"
                     /locus_tag="PKL93_02345"
     ncRNA           23143..23470
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKL93_02345"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23489..25231
                     /locus_tag="PKL93_02350"
     CDS             23489..25231
                     /locus_tag="PKL93_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNQ70135.1"
                     /translation="MNIPMTRSDMVERGWECLDIILITGDAFVDHPSFGTAMIARVLE
                     SQGWKVGVISQPDWRTNEDISVLGKPALFFGVTSGNVDSMVANYTSTGKRRRSDDYTP
                     GGFGGKRPDRATIVYSNLIRQVFKETRIVLGGIEASLRRFSHFDWWSNRIRKSILLDS
                     KADLLVYGMGEKPILEIATKISKVREVPTDVEGTVFWSSSKPAGIELPSHEEVSLDKK
                     KYFEMTKVMHEETDPVRGHQLFQLQDNRYVVQNKPPKVTSEELDAYYSLPFTRRVHPV
                     SLSRGRVKALETVRYSVTSHRGCYGQCNFCAIAFHQGRTVVSRSEDSIIQEISSFTRD
                     VGFRGTVTDVGGPTANMYGYECSIKEKVGACAKKRCLFPEVCDSLKPDHSRYLNLLRK
                     IGKIPGVKHVFVSSGIRYDLILKDKRSGRDFLKQLLKNHVSGQLKIAPEHTVQKVLEY
                     MGKPSKDVLLDFLKEVRLLSNGKCHIIGYFIAAHPGCTKEDMIELKRFVKREMHYNPE
                     QVQLFTPTPGTISTAMYFTEMASEKGDPVLTERSTGGRNRQKEILVRKEILDRKPEKD
                     IKGTGHKRNKSDRH"
     gene            complement(25163..26428)
                     /locus_tag="PKL93_02355"
     CDS             complement(25163..26428)
                     /locus_tag="PKL93_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNQ70136.1"
                     /translation="MFVLVVFISAYYLIISGKFNRSTVAFAAGILILLSKVIPDFDMK
                     ELGYLVDFNTISILIGMMIVVGTLRTTGFFEFVAVHVVRFSKGNVRALLTFFVVTIAL
                     FSAFLDNVTTILLFAPIIFLVADALEVSPRTFILAGVLAANIGGTATLIGDPPNILIG
                     SASGFGFMDFIRIDGPITLFALIIALIYLDRHVFKDYREMGEKLQRLANMDPKKAIVS
                     MSAFYKSLAVFFATIVGFLLHGLIGVEPSLIALAGAAAAMILNGKSFSHLSEDIEWDT
                     IFFFMGLFVLAFALQEVGITSVISDLLGSLSGNKILLFLTLYWLSAIMSGFIGAVPAV
                     TFMIPVIQGMTTKFGVPADIWWVISISACLGGSFSIAGAAANMVGVGLIEKHSKERLT
                     YGDFLRFSMPVTLITLVAGTLYILFRFSI"
     gene            26562..27962
                     /locus_tag="PKL93_02360"
     CDS             26562..27962
                     /locus_tag="PKL93_02360"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNQ70137.1"
                     /translation="MRKVLAVLIVVILSVASLALVNEGYESPITNVVQAAGPAVVKVD
                     VEATRKYSITDPYGFEDFFRRFFGEIPGQKVTGVGSGFIFNKDGYIFTNEHVVSDAEK
                     ITVSLLDGSTYPAKYIGGDEELDIAVIKIDPDGVDLPTVELGDSDALRIGEWAIAIGN
                     PLGLKHTVTLGVISAVGRQLPKPDGNGVYSNLIQTDAAINPGNSGGPLLNIHGQVVGI
                     NTAIIAPEVGTTLGFAIPINVALRFVDSIIETGSVQRAYLGVYMDTVTEEVSKSLGLK
                     VDSGALITDVVPGSAAEKAGIKPQDVIIGFESLEITNSAELRAAVLNYPAGSEVKITI
                     DRFGERIVLTAVLGSLAQESESSATEDNGARQAFVSSLGISVSEITPGDRERLGIPQE
                     FRGVIVREVNPEGIIYRLGIGPDDIITRLSINGNQESIESVNDFEKYTEKIKKGDYVA
                     FFVYRNGVRFVASFQL"
     gene            complement(28113..28520)
                     /gene="fabZ"
                     /locus_tag="PKL93_02365"
     CDS             complement(28113..28520)
                     /gene="fabZ"
                     /locus_tag="PKL93_02365"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNQ70138.1"
                     /translation="MRDKEFVKERIPHREPFLLVDGVISMTDSEILAFRDLLENDPVF
                     EGHFPGYPIYPGVLILEGLAQTAGILLLKPNETPLFAGIERARFKTIVRPPCRIEYSV
                     KLIDSRMNVAKLETSAYVEGKLAAKAILLVSSI"
     gene            28651..29604
                     /gene="fabK"
                     /locus_tag="PKL93_02370"
     CDS             28651..29604
                     /gene="fabK"
                     /locus_tag="PKL93_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HNQ70139.1"
                     /translation="MNEQRQSLTEMLGIKYPILQGGMAWVSEHNLAAAVSNAGCLGII
                     AGGNLSPEDLLHEIREIRKLTSKPFGVNVMLLSPYAQEQIEIICRERVPVVTTGAGSP
                     SSFIEKLKTAGTKVIPVVASSGLARRLERQGVDAVIAEGMEAGGHIGKVSTMVLVPAV
                     VSATRIPVIAAGGIADGRGLAASLALGASGVQMGTRFICTTECRAHSRYKERILLSNE
                     LDTVVTGNSNGHPVRGFRNRLTRRIEELEKQGSGFEEIEKIAVGALKRAVENGDISDG
                     SMMAGQSCGLIGEILDVSQLVQSIIDEAEETIRFLGGIEKW"
     gene            29598..30533
                     /gene="fabD"
                     /locus_tag="PKL93_02375"
     CDS             29598..30533
                     /gene="fabD"
                     /locus_tag="PKL93_02375"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNQ70140.1"
                     /translation="MVAWIFPGQGSQYVGMGKSFCDESPLFREFLELSGQLLGFDMLT
                     MMLEGQEDELKLTHNAQPAILSLSIALSDALIERWGKPDICAGLSLGEFSALVVAGTL
                     RYADALRLVRLRGTAMQQLMSSEDGTMAAIIGLPEKVVEEICLEISSSEEVIVANYNC
                     PGQVVISGLSEKVRSAMNKAMEKGAKKCVELSVSAPFHSRYLRPVGEVLREFLNGIDV
                     KPPEIPVISNVTGELFPKDPNAIRELLIEQAYSPVKWEQSIRKMIELGADRFVEVGPG
                     KVLSGFMKKIDRSVSVESTENEGLGILKELREIKN"
     gene            30560..30808
                     /gene="acpP"
                     /locus_tag="PKL93_02380"
     CDS             30560..30808
                     /gene="acpP"
                     /locus_tag="PKL93_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNQ70141.1"
                     /translation="MEKTSTIQEKVISVVADSLGVEAGEISGNSRFIEDLGADSLDLV
                     DLVMELEDKFEVQISDSQASSFRTIDDVVKFISTLDEK"
     gene            30810..32033
                     /gene="fabF"
                     /locus_tag="PKL93_02385"
     CDS             30810..32033
                     /gene="fabF"
                     /locus_tag="PKL93_02385"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNQ70142.1"
                     /translation="MKRVVVTGMGVISSIGTNLTEFWESLKSGTSGIDWISTFDVSEY
                     GAKVAGQVKNFDPTKYMDRKDAKRNARFVQMAIASASEAYDDSGLGNSRINSERAGVI
                     YGVGLGGMDVIEDQHSVLMNKGPSRISPFLIPMTIANMAPGLLAIRFNFRGTNFTLST
                     ACASATNAIGEATRLIRGGILDIAMTGGSEATVTPLALAGFSNMNALSRGAPKTASRP
                     FDRDRDGFVMAEGSATLIIEEYEHAMKRGAMIYGEVVGYGSTDDAFHITSPEESGDGA
                     YRAMKMALEEAGVDPSEVDYINAHGTSTPLNDLMETRAIKRLFGNRQDLNISSTKSMA
                     GHALGAAGAIEAVATLLSMKNSFIPPTINYSSPDPECDLNYTPNFGVKKELHFVQKNS
                     FGFGGHNASLLFKKL"
     gene            complement(32157..33113)
                     /gene="trmB"
                     /locus_tag="PKL93_02390"
     CDS             complement(32157..33113)
                     /gene="trmB"
                     /locus_tag="PKL93_02390"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNQ70143.1"
                     /translation="MSEYYLRNYVDCTEYKLPLDLQDVFGRKRETFLEIGFGSGEFIL
                     QKAIENPNADYLGVELSMISAKKLLKSLSRNHVGNVRALLVDASFALRNILPKDSMSG
                     VYMNFPCPWPKKRHSERRLNSVNFLEKIAKVLKKGGFFQLYSDSKEFVHEMFGEVGKT
                     NCFDDPVLEVNPTVGVGTRYEKKWLDMSKEIFRMKCIRIKCEINGEEDVVRVSNLWID
                     DIDEKSLQKVDGKSFSKNEIFVKFMGLYRNMDRNTFLIETLTVDRDFSQRFYINLSRR
                     GNRWLIKLDSQARPFRTKAVKFALRVLSEKIAKKDPGGDENH"
     gene            33282..33815
                     /gene="hpt"
                     /locus_tag="PKL93_02395"
     CDS             33282..33815
                     /gene="hpt"
                     /locus_tag="PKL93_02395"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNQ70144.1"
                     /translation="MLFDPARIEVLYSEEELKKIVNGLAEKINEYYSPITDEITAVCI
                     LKGSVHFYSDLLKRLNLKVRYNFVHVSSYSGASTTGRIRVKTWVDESVTNRYVLLVED
                     IVDTGGTLRYIINYIWKQKPADVKLVSLFEKTIHDHGVKIDFTGEKIGDQFIVGYGLD
                     YEEVCRNLPYVGYLKGE"
     gene            33815..34126
                     /locus_tag="PKL93_02400"
     CDS             33815..34126
                     /locus_tag="PKL93_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70145.1"
                     /translation="MKKGNVVTLVLAVLLLSICTITSLFALNVVSSNRKNTQLMLEAS
                     IMRGVRVSAEKLLLFSMEHGKKLAVEINGYHLETDEINGSWCVRLDNGDKEEIIFAEG
                     R"
     gene            34123..35019
                     /locus_tag="PKL93_02405"
     CDS             34123..35019
                     /locus_tag="PKL93_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNQ70146.1"
                     /translation="MSDLIAISVDSGCAPSRDFVREHKLFFMGMKVIIDDKEYTDDFD
                     FMENVFYRIVENSKEFHTAQPPLGQVLEYYNDIRSRGYTELLDIHFSSKMSGVYETCV
                     MASKMVEGIDVRVVDTRKVSIGANLVARKLIKLVETGCSLDEVFESVPRVIDNTFMEF
                     SVPTLKYLIKNGRIGKAQGLAGTLLNIKPILSVDEEGFISPIAKLRGMKKLQEQMVQN
                     IVEFLQNRRKSITLYSIYGSEEYLTVMHETTEWLIESIESRLGISRSDIELVSGRIWP
                     TIACHSGPAVFGLACYGEREAE"
     gene            35019..37376
                     /gene="recG"
                     /locus_tag="PKL93_02410"
     CDS             35019..37376
                     /gene="recG"
                     /locus_tag="PKL93_02410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNQ70147.1"
                     /translation="MLLEQFLEECERLLEAHLSGKREDNVLPELKRFLSLLDRSELSH
                     FSSLGEYLSKFISYLSRIDEFPHDRKVKRLKNGLEMIAKLRNFFLLHNSEEIESLLGR
                     PVSPSTPIKYAYSVGEARSKILKRMDIETIGDLIQYFPRDYEDRRIIMPISSIVPDRK
                     VSVKGRLLNFSAKKASGYTIISAVVSDGFGQLLLKWFNQDYIIQKLRRDREYLIHGLA
                     KETPFGPMEMNSPEIEEIQGEVPREILPVYSLTSGISMKMMRKIVKRNLGLVRSLDDL
                     VPSSITTERGLLPRKHAFTAIHFPKSLYEIRKARESLAYEEFFLFETTILFRKRQIRK
                     EYQGLQKEISGVLSKRLIESLPFELTKDQVTAFEEIRDDMRAASPMNRLLQGDVGSGK
                     TLVAELAMVDNYEAGYQSALMVPTSVLAMQHYEKIKRELSPIGIETGLLTGSLKKNEQ
                     DFVRMRLKKGEIDVVVGTHALIQDGVEFKNLGLVVVDEQHRFGVKQRETLTTKGKLLD
                     SLVMTATPIPRTLALTVYGDLDISTILTLPKGRSPVRTIILARKRLKDLYSYISDELK
                     MGHQAFFIYPLIEESEQVDLKNATDEATKLREEVFPGVGVELLHGRLNDNEKQEIMQR
                     FRSRQSMILVSTTVVEVGIDIPTATVMVIEHPERFGMAQLHQLRGRVGRSNLKSICVM
                     VMNKAISDDALSRLREFASTSSGFDVAELDLRLRGPGEFLGLRQHGMPQFLIGDIVND
                     RDLLFKAREDAKQLMEEDPELLEHGALRIEMERVYSERARLIEVG"
     gene            37378..37935
                     /locus_tag="PKL93_02415"
     CDS             37378..37935
                     /locus_tag="PKL93_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNQ70148.1"
                     /translation="MLTITGGKFARRMINPTTAKKTRHTPQLARKALFDVIDINSKSL
                     LDLFSGSGIISFEAISRGAANVLAVEISRRACNSILGNKRGIDPSMDLEILCSDFRRA
                     IPSFVKSGIAFDVVFADPPFDEEYIQPLMAVLERNTELLNLAGMLIVESSVREKGFVL
                     QGAGSFDLVDQRNYAGVVFSFMVKK"
     gene            37932..38408
                     /locus_tag="PKL93_02420"
     CDS             37932..38408
                     /locus_tag="PKL93_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNQ70149.1"
                     /translation="MRFLVEEPLTKLARKLRLMGFDTEICSRNEIGKSFENRDVFLTK
                     SKKAMEIAIKFGIRSYLIKSENWRSQLKSVIQRFQIGLDDVHFLSRCSKCNGLLEEAS
                     LKDVAMVPDYVVLTAERLYKCTRCGQLYWNGSHVQHMKWMNEIAGKSGGNAEENKF"
     gene            38386..41097
                     /locus_tag="PKL93_02425"
     CDS             38386..41097
                     /locus_tag="PKL93_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ70150.1"
                     /translation="MLKKTNFEKSSDEVIQRILKKISSEPSSKASLFILEKYRNVFKE
                     VNPDEAFTRTGREIRLSLNPSLKELSEDFSGEMNVVVSSHGKEFREKVFLVSLSYDGE
                     PHGLLIMPAGAESNKSLKDWIKEIELALHYSLKRSAEEEAFRRLKLVSKLTSVLETET
                     QVEELIRKSLEIAREMLRVRWIFYLEGQGKSLHLKAAVGNGNNPLKGIEIEISDDQLS
                     ELASEKLFVSARKSDLSSAIFKEDVSVGSFMAIPLAVEGTLNGLIVAADREDIEGEFR
                     AYKHLDKEDLKILDDISRRMAVAISRIRLTKRLELEVRKLRQLGKKHEELINDQKDQL
                     FKLGVLHKITEAMKRSMDRERIVRILLIGATDSSGLRFDRATYFRVDSGRSLLILETQ
                     VNSTRGNSFKDRSAYSDLTRYLQDVSLEAAQSTTNNEPSASISYPGNIILERVILRKK
                     MVHVSPEMLRLRGEELSLMNRIIGTDEYLIAPVSGEEEVQGVLVVDNAYSRKKISSGD
                     IEILGLLADSTGVALELTENYKRIVQMAESLEKERNLSNYYRRFVSSILQSLESAIIV
                     CSVDGEITEINRTAGFLLNIEREELIGKKIQSMKPQLGSIIDLLSDAMRIGETITLTD
                     QYFEAFGERYFDVRITPLREEKRKGLTGVILSLDDITRRRNLEQELKGREKLAALGEM
                     SARVAHEIRNPITIIGGFVKRILASSDRKKTEEYAKILMDELQRLDRIVGEVLEVSRR
                     KAKPSKEAFDLVSMTREILAGFEEKAASENVKLVLYTKEERIEYYGNRDKIKQVIINL
                     VQNAIEASESGDEVHVTLEKDSEEIRFSAWNGGKTIPEETLKRIFEPFFTTKTLGTGL
                     GLSICKKIVEEHGGSIHASSGSEGTVFTFRLPTKEFGRFDLEKSTDS"
     gene            41075..41440
                     /locus_tag="PKL93_02430"
     CDS             41075..41440
                     /locus_tag="PKL93_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ70151.1"
                     /translation="MKRVLIVEDEKNLRLLIEEELTESGFEVKSTGTAEEALEILSTN
                     NSFDLMTIDIEMKGMNGLELAGVVRKRFPNMKIVLLTAYTHYKHDLSSWAADAYVVKS
                     PDFSELKKVIESLLREDLD"
     gene            41460..46184
                     /locus_tag="PKL93_02435"
     CDS             41460..46184
                     /locus_tag="PKL93_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNQ70152.1"
                     /translation="MHKKIISLSIVLLITMSLLGYSVRPGDLLSISVYGSSDLSSETL
                     VGPDGTVSVPPLGSIMLLGKTLEEIQSFLSSQFLSKGILTSEPQVTVSVKSFAPFMFY
                     VLGEVNKPGAIEWRDETIGISQLLALAGGLKDGADLSSSFIVDSEGTRNYIDLSSILS
                     GGSALKEKISEGSTLFVPTGPRAWIMTMGEFNSPSLVKYSPGITLTQVIAKSGGLKET
                     ADDNEIMILSEAKENSSIVSLNSVLSGIKSDPVLSAGSIVIANDASSKSVKLLGEFGN
                     PSSIPYREKLTLLQAIGEAGGVNQAASDQLYILRKDLDEPESVEISKLLSGQIKDIDL
                     YPGETVFVPRQPERYVYIASASFGGKVEFSIDEEITLINALVKVDLFDPVSQDPLVVV
                     TTSGERREFHQGEDAVLDAGSMIILPGGEKSIFVTGEVKNPGVLSFSNFEEITLGKAI
                     AKSGGVTEFAGSVELLENGEAKKFSIEDAVKSTLVLSAQSIVNVAEKERRFVYVVSSA
                     EGGRIDFEEREPFTLRNLLAKMNLLNFNSKKEISIIDTAGMSKFLSVSTLSKEDYQLE
                     TGSIVLIPDISTWIYVFGEVERPGKIKLDEDDLLLTRAISASGGFTQNADISSVQVLS
                     GSEISEIDLDEVLKGISSDREISYDDLIYVPRIDSRFVYVVSEENGGRVDFSSGESIT
                     LRNALAKLNLVEIASQKEISVISPSGKEIRVSISSLERSDLELETGSVIFYPQADNVA
                     NVIGQVRNPGTVNLKPGIPFNLSTVIAAAGGITDLGDEFNITVIDSKSASTAVYSLED
                     LTRDPIPVIPDGATVFIPELEARFVYVLGGVREPGIKEIGKEEPNPTLTKLIAISGGL
                     SDPLSKEVEIIEDNQRRKLDLQSVMMGRIQDPQVSNHSIIYISETPDRFVYVISKEKG
                     GRVDFGSNEEITLRTALAKQNQLDFDSDGRVTIILPDGTKRERQISDLGESDMNLSPG
                     SIVIYPDVLRQVFVVGAVNKPGLVEFEPGEKVSLTTVLARAGGMTPLALSSKIQITDP
                     FGRTSTVSVDSILLGKALDMNLEDGSFVYVPIYEPVRVNVIGEVKYPGTIEFSKTELP
                     SLILAISRAGGLTEKAAQNVKITGEEKEVLWLDLVDGKDVMLVSGQTVYVPGDDRYIY
                     VGGEVNSPGKFEFAAQEEITVLRALARAGGEKATASDLVKVLLPSGEILPVSLSNIRN
                     EGKDAVIEPGSTLIVPKQVLRITVLGAVKNPGLFTFNPDEDHSLSDVIARAGGLIDES
                     LVDAIIVHDDKRYNEYTPEVLQKRGNQLSDNTYIYVSSRSPVSVTVLGDVARPGEYEI
                     SVRNPSIATAIAVAGGVEKAVTTLSLIGIDGRVQLFDALRTEELARSYLEDGDTIVAL
                     RNYDSFVAVVGDVESPGVFPVSNYGELTLAELISLAGGITNFESGGRVFISSGGKTEE
                     IIISPDDFIALTRKVVPAGSTVYVPYTEPARVYVFGEVQKPGIVRYYDGMTAIEALVE
                     AGGPTSYAVLGNALLFQDLEEEPMVIDLDQQKGKPAKGNIPLIPGNIIFVPQSPIVNI
                     KDIMSIVASSLSIVNSAVGIFK"
     gene            46188..46859
                     /locus_tag="PKL93_02440"
     CDS             46188..46859
                     /locus_tag="PKL93_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HNQ70153.1"
                     /translation="MRNLNIDSHCHLLPAVDDGVEEIEESLAILEEMKLKGLQRLYLT
                     PHLFSPISPSDPAEIMRRWHEFRFELNNHSVEVLLGSEIFLRPEVLESDLITMGESNF
                     LLVELSTQQKPHYLFEVIGKLQSTGFRIILAHLERYHYLFKKIGFIFGKVEPTDEIHR
                     LRDMGVLFQVNWNSLDKDSKARVLVDRRIADITGSDKHKQGDNRLLIDFDDDRYRLFL
                     NDSFL"
     gene            46874..49084
                     /locus_tag="PKL93_02445"
     CDS             46874..49084
                     /locus_tag="PKL93_02445"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNQ70154.1"
                     /translation="MSEFEGSDYRELTLEDILRMFKKRMILFVSIVLAVVVVTGIYLI
                     FATPIYEASVTIKVDPTSQSSVSDLFSSSLTGSSGSNISTEVELIKSRTNIEEIIEVL
                     DLVDRIYSEETKNRLLAEGYSQKDLVTSLTRTISSMITVSPVKDTRIVRVSVQNRDPI
                     LARDIANTLADVYNTKLAELSKRDLTRKREFIEAQIPLLESDLNEATDRIKDFKEETG
                     IYVLDKHADLLFQMLSSYDKQYNELMISAEEKKAEIETYQSMLDDFDSNDSKSIKALW
                     VQTSESFSVNPVLTSLRQSLATLQVELASLEEQYPKTDPRVRSKITEISKTESLIEEQ
                     IQNEFITSGQGMTLNPAYQQIMTGVISSEAGFQILQASIQAVGLLRDQYQSELRSLPA
                     KEQQLLDLERQIAVKESLYTLLLERLEEAKISEAAVVGNAAIVDPATVPQSAVKPNKK
                     LSLAIGGVLGIFLGMLMVFLAEYLDKTLKTEEEIERFSRQPIIGRIPNIEGASEEMYV
                     EKNPTAPSSESIKLAASNLSFTMGEGKSVAVTSVLPTEGKSFVIANLAYSMANSGQRV
                     VLLDLDLRRPRVEKILKAEKITKGAVDVIMGNTTIDEVIQHYAVNMDFIAVGTIPPNP
                     TIVLSSKNIDALLSELKQRYDKVLIDMPPAVVTSDVSLIGNKLDGIVLVVRPGRAIKD
                     GLRIVVENLKTVGIKILGVIVNGVDEKNSSYYYHYYYYYNEEGKRQKRRKRTKK"
     gene            49201..51975
                     /locus_tag="PKL93_02450"
     CDS             49201..51975
                     /locus_tag="PKL93_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ70155.1"
                     /translation="MSVKKHVIDFETIVYLFLTLFIPLFVTKGFTHEPSTGKHLFYVI
                     GFTIIFLSVLIRKKEISVEFGDVHLAFFGIGIAALLSLIVVSMDNPQYLRYSLEIALY
                     VVFLSFTAVYISNKWNTIEKLEIVMIFFVIGAAVVAFDALLNFYLGVDIFLGKVGEPF
                     ARASARSTIGNPNFVSDYMGMTIPMIFYFLISRKPLGFLLKRHFGQLVFKITLLIFLI
                     PMVSSVFVSQTRTVITAIFVGNLLFLVLYFFLKKRKKKEALEDSESRKLGRVSLVFFV
                     LALIIIAVLSYLYLTPSPLSGDGRINITARLEYAITSSGSWNERFSAWYNSIFQWLES
                     ENKLRIPFGSGIGTFQLYHLLYSPDVLQHSPYFMPVWNNFKRTHNDYVQGLGEMGLIG
                     FLFIVLLVGLLVFRYLKNLLKIDNRRDLLLYGSLGAGIFSLAVHSFFEFPLHMQPNLM
                     LAIFLGSVVVGKYFNPDLKNKKISRLPFAVVIMVLAGVLIFLKTSAFIGEGFFRIGQT
                     NQQYYLAYYNQAQSLNIKALEQAKNEINSYAGNYAYLKDVTSYMSAKGSEIRSKYPGA
                     SQIDLLELAEKDRQSEVRKLLDEIDNRINQYNFYLARSGEYYEKAIENFKFSNRVYPV
                     FGKPLWYIAGLGTKTQRLETAKDNPELMKSILTGKDEYSSDIIPEFKGNPKIIPVHRT
                     TIRTLPFRDFFEKNISVFDNPEYVSGLQLYFITQIQMILDAADYYESSVILFSERQTP
                     RILGRLYTSINSELKKYYSFISSRETMMTSAFGESGEFRQLLIDLVYESADRAIYWFD
                     LAISLLPGTWNRYPDWEDIYIEYMNSIPSVLDSNEERKLKILEVAEKHVWACENMGPE
                     APVETLQFAIQWGKSNLSNGELIDFEQSLKKIYEEVVSLNTDLLEKSPNIPQDTAERI
                     RTLISLYEAL"
     gene            52036..53943
                     /gene="metG"
                     /locus_tag="PKL93_02455"
     CDS             52036..53943
                     /gene="metG"
                     /locus_tag="PKL93_02455"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNQ70156.1"
                     /translation="MEKFYVTTPIYYINSEPHIGSAYSTIVADVIARYRRLSGYDVFF
                     LTGTDEHGQKVLNEAIERNVEAQKYCDEMAEKFKALWKELELTNDAFVRTTDEYHMKT
                     VQTFVSKLKENGDVYKGKYEGWYCIPDETFWNEEDLKEGRTCPECGREVKWVSEENYF
                     FRLSKYNDILLRFYKENPQFVQPDFRRNEMLRILESGLKDLSITRTSFDWGVPMPDDP
                     EHVIYVWVDALINYLSAIGYPHNIDHFERYWPADVHLIGKEINRFHSIIWPAMLLSAG
                     LPLPKQIYAHGWLTVNGQKISKSLGNAISPVAFVRTYGNDALRYYLLRDIQFGRDGDF
                     SETNLISRINADLANDLGNLLHRTVVMIQKFNDGAVPERSITETIDEDLVELGEKTFS
                     AYTESMDNMRFTQALESVWELIRFGNKYIDLTEPWKMGKKPEMKGRLDTVLHNLVCTL
                     KNVSIMIEPVMFRTAREIRKRLGFGGTMTLENVSWSDIVAGNRVSHGEPIFPRIEIEK
                     HRKVTEMENKETGPEIKDNLLISIDDFSKVELRVASVTEAEIVPKSRKLVKLQLDLGD
                     LGRRQVVAGIAQFYNPEELVGLKVVIVANLQPAKLMGIESNGMILAAKIGDSLSLLTV
                     HKDVQPGAKVS"
     gene            53965..56391
                     /gene="gyrA"
                     /locus_tag="PKL93_02460"
     CDS             53965..56391
                     /gene="gyrA"
                     /locus_tag="PKL93_02460"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNQ70157.1"
                     /translation="MPEEIIHKNIDDELINSYMLYSMSVIVGRAIPDLRDGLKPVQRR
                     ILFGMSELGLHHNQGFKKSARIVGEVMGKFHPHGDAAIYDALVRMAQPFSMRYPLVEG
                     QGNFGSIDRDPPAAMRYTEARMETLAEELLQDIDKNTVPMMPNFDGSLSEPDVLPTKI
                     PNLLLNGTSGIAVGMMTSIPPHNLVEIVDAISLLIKDPECSVSDLLRHIKGPDFPTGG
                     MIMDAASIPSIYQEGKGRITVRAIAEIEESGGSSQIVVSEIPYNTSKADIIEQIVNAT
                     QNHRDLQIRNIRDESDQKGLRVVIELKRGADPNVVLNLLFKHTQLQTTFSVNMLVIDE
                     KKRPRVMNLKQILQGFLSHRFNVIRSKTEYDLEQASKRAHIVEGLTKATRSIDTVVDI
                     IRNSANSEEASKNLMETLEVTQEQSSAILDMRMGKLTGMEIEKLLNEYKDLVAKINEY
                     RMILASDEKVYGIILDELNEIKAKYGDARRTRIDLGNGTDFNIEDVIPDEDIVLMVTK
                     KGYIKSTPLESYRKQGRGGKGVIGVRTGDEDFVVNILTTTRLSKTVIITSKGKAYVLS
                     NHIIDHSSRDSKGKLLANYIKIDPDENVQAVLSTRKEEVQGKQLVITTRSGKIKKTEF
                     EAFLNVRISGIKAISLNEGDRVIDASLTSREDDTIIISTKNGMVIRFPLDQVRSMGRS
                     AAGVMGIRLKKNDEVVGANIVASEDTRFLLTATEKGGGKRTCLSEYRSQNRGGLGVKN
                     IYGLEKIGKVIGTLVVNSDDEVILATKLGMSIRIPVSQVRPTGRVTKGVRIVDLKDND
                     IAASMAVVVD"
     gene            56425..57051
                     /gene="lexA"
                     /locus_tag="PKL93_02465"
     CDS             56425..57051
                     /gene="lexA"
                     /locus_tag="PKL93_02465"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNQ70158.1"
                     /translation="MTLTEKQKKILEYIENFIKLYGYPPSIRDICRDFEISSPRGVAK
                     HLESLEKKGYIERTGVSRGIRVLRSPDGSSLEPGEDVVMLPVIGNVAAGEAIQAVQTE
                     DEKIPVPLWMIRRGFEYYMLKVTGNSMIESHIVNGDFVIIRKQEWANNGEIVVALIDD
                     EYATLKKYENEGPKIRLVPSNPEMLPIVVEANRVKIQGRLSGVLRWYK"
     gene            57079..57540
                     /gene="rpiB"
                     /locus_tag="PKL93_02470"
     CDS             57079..57540
                     /gene="rpiB"
                     /locus_tag="PKL93_02470"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNQ70159.1"
                     /translation="MKIAIGSDHAAYELKSFLVQYVESKGHSVIDHGPESGSLSVDYP
                     DFADKVCKSVTDNDADYGILLCGTGIGMSIAANKHRGIRAALCLFPEMAALSRRHNHA
                     NVLVLGGRLIGSELAGWIVDAFLNSSEEGGRHQTRVDKLETDTFENRVNEP"
     gene            57537..58499
                     /locus_tag="PKL93_02475"
     CDS             57537..58499
                     /locus_tag="PKL93_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNQ70160.1"
                     /translation="MIVFYDRRHLFHLPMKELDNGNWIENPDRPERIESVRSALESAG
                     FQIKEPRDYHCSHLFQVHTPEYVEWLKAKSNSVSRDREYFPEVFGYDKLFDTGTPVTS
                     GCYVSALASVSTALNAVDSILESEPVSYALCRPPGHHAGKSTGGGYCYFNNAAIAAKY
                     YQKHTRGYVAILDLDFHHGNGTQEIFYYDDTVFYVSIHGDPKNFYPWISGNSWEIGSD
                     SGEGFNMNFPLAGGIEGPEYMRTLSKALQEIEDFSPDLMIISLGTDGHKDDDIGHFNL
                     SSEDYSMMGKLIGEIEVQKLIVQEGGYNPKANSASVLNFLTSIR"
     gene            complement(58543..61014)
                     /locus_tag="PKL93_02480"
     CDS             complement(58543..61014)
                     /locus_tag="PKL93_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNQ70161.1"
                     /translation="MSVRRLFLILVLVLVSGSVFAVIVPSRITVLDNRVISDGEVLEI
                     LDIQRGKEISDSELSEALERVRKSGYFSDVYYSFDESSGLLKIQVVEYPVVDVEVVFD
                     GPKIVEPDALESVSVIESGKPADPSKLIYDIPLTQEAMMSKLEENGYIEPFVEVEWET
                     DKSRKDVFSGNIKDRVKVTFTVKVSFLWEVSIEAPLSDESKAFLSSKLQIDCLKKYYD
                     TSFLVRWINSKKKYVPKLDDINMMVQTVYSTYYANSSGQWGFDDETYQAMILTLNNAL
                     RNARQVTLPEGVKESRALSMSIAFAPVEYVKEEFDLRSVFIEGNVNLQKGEILRETGL
                     TEGQRVDNEVAMRSIQAVQDLYTERGYPFIRIEPVIDEKIGFFSIKITEPLVRDITVE
                     FDGPKKTQDYLIVDKIVIQKGNVLSLDELRNTYAFLNNTGYFSKVDIAPVPVGEDALD
                     VSITLQETDKNNKIGGGGGWQNGLNLYLDLGLLNVWGYGQNISTTLSVTLPMPNNKEV
                     IVTDGATETTTRRPAFDATIGYSIPKVGGTKLDLDTAFKFKFSGFTTTNDSTDTIVTK
                     SSQETEFLFSLTPIYNVSPGQKVGFTAGYEYIMSESQVSTETKTATEVDTGSLSENFD
                     GIFTGLSYELSTRDDLYRPTMGSEYLAGLTVRGLFGKDNEPFISGYAEYRNFMSIGDP
                     VLGFRVRTQQLFPLSPNGVFRSYLLTPDTFVRVRGSQIIGQNAYGVTVGSAQLRYPLT
                     PEASSIPLDIVGFGDLLFYRKTPGGDLIGGNFLADFGLCIDISIPMIGLVRLGYGYNT
                     FLAEQNSGAGKPYYGTPFFGFGAAF"
     gene            complement(61004..61567)
                     /locus_tag="PKL93_02485"
     CDS             complement(61004..61567)
                     /locus_tag="PKL93_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ70162.1"
                     /translation="MGLRTICDHIIDIAQNAVDSGTEEATLRIVEKREESFYFSVVDR
                     GKGISSESLESVLDPFYTEKRKKVKFGLGLPMLKFAAESTGGEFRIRSVEGSGTEVSA
                     KFLLSSIDCQPVGDVPLTLFAVITLSDRVDWSIERLLDDEGYAFSSSQFREAFGNDCF
                     TSPVKMKMVLTILQEAEISLGGLSDVR"
     gene            complement(61558..62277)
                     /locus_tag="PKL93_02490"
     CDS             complement(61558..62277)
                     /locus_tag="PKL93_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNQ70163.1"
                     /translation="MSFLCDFHIHSCLSPCADITMTPNAIALTCVSKGIDWVAITDHN
                     SARNVRVFSKVLNRFGVVVIPGIEVHTLEDVHILAYFPEVEIAEDYSEWLKEEKFSRL
                     SVDPEISGYQLIVDEDDTFTGFEEIWLGQPALLSISQTLSTVEEYGGISVMAHVERKM
                     GLLTQLGFIPETCREVPMEISFKRTLEKKLPVENIIHSSDAHSLSMITPTVSIESKAR
                     SFEEFRSALYSNGRSLRIIWD"
     gene            complement(62284..62619)
                     /locus_tag="PKL93_02495"
     CDS             complement(62284..62619)
                     /locus_tag="PKL93_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur binding hydrogenase"
                     /protein_id="HNQ70164.1"
                     /translation="MKLNDLIEKCGMRVITSLPLDIEISDAYIGDLLSDVMGNAPSNS
                     IWLTVQSHVNILAVASIVGVRAIVLCNGLHFEESTVRKAEETGIVLLESDYTTFDTAL
                     KLIKSGLRG"
     gene            complement(62621..63580)
                     /locus_tag="PKL93_02500"
     CDS             complement(62621..63580)
                     /locus_tag="PKL93_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ70165.1"
                     /translation="MTERNVDDLLDKLRGLFADITAKDIMIKSVVTLTKDRTMWQAKE
                     LMRICKISGIPVTDGNNQLEGIVSIEDIIVALEGNYITDPIEKHMTRNIVTFSPEMKL
                     ESVIEMFNRYRYGRFPVVDSDGRLVGIISKKDIIGAILDKFRLIYVHDKRRREVLEQS
                     VDWFDKSLITGDYVEKTSADFVYHIDYTDIDTAGVGSAKLKSFLKTLTDDDTLIRRVS
                     IACYEAEVNVVIHSGSEGYIFAWYDENVTKVRVEDYGRGIENVEQAMKEGFSTASDHV
                     RELGFGAGMGLSNMRRYSDKMVVVSEAGKGVVLEMHFYRNGRK"
     gene            complement(63573..63935)
                     /locus_tag="PKL93_02505"
     CDS             complement(63573..63935)
                     /locus_tag="PKL93_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70166.1"
                     /translation="MTLSRIIEILGCEILYCGDGDPEVESVGAADLMSDVLAFGGPGM
                     ILVTGLNSPQCVRTASVVGATAVVIIRKRNVPETTIELAKELDMPLVHARMSMYRACG
                     ILFNENLGDSLEEGKHFD"
     gene            complement(63938..64627)
                     /gene="thyX"
                     /locus_tag="PKL93_02510"
     CDS             complement(63938..64627)
                     /gene="thyX"
                     /locus_tag="PKL93_02510"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNQ70167.1"
                     /translation="MKIDVLDRGFVELVDKMGDDYSAVQAARTSYGKGLTNKERDDRL
                     IDYLMKNGHQSPFEHIIFKFHLKLPIFVMRQLVRHRIASINERSGRYTEFSEEWYIPG
                     VIRIPDKDNRQGSIVCKDKELNERATSIISEAYENAFAAYKRLIEMGVAKEMARIVLP
                     TSMYTEAYWTVNARSIMNFLNQRAHSHAQAEIQEYAIAIAEIFKTGCPVTFDAFIRHS
                     YTGDLLNREAQ"
     gene            complement(64617..65789)
                     /locus_tag="PKL93_02515"
     CDS             complement(64617..65789)
                     /locus_tag="PKL93_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70168.1"
                     /translation="MKSAFAVAIFLVSVSLIFALDNAELLLSKEYLIELQSLETQSVE
                     TKAILAIAVGLKYRETIQPNYKVWFSNLYEELKDKALPSEIEEGIKIVNELVSVSTVS
                     ALNMLYSTQNSDNLIKAISLRAFFYDWVDTRDPRSSEEIVKTAYELIELLPNQYFPYK
                     ALLEVYSSGSSIDKDRLMEIRERIFSEGLQDLLGDDLIESEFVSGLEELVLEDYSRLG
                     TDEPVSMYYAAMAYMKMGESSEALEILNSIDLHRIPPRFASDTSMVVGNYYLGNGDFE
                     EAVKNYQDSLRFWPENSMAVRNLGMAYYLMKDRDYYDLARFYLQLSGYESFDDEVSSA
                     LKELRRRAIFELALVTIVPLAAIVVVGLFLLEYFSKKRRTSQERKAMKEDGGNNED"
     gene            complement(65786..66580)
                     /locus_tag="PKL93_02520"
     CDS             complement(65786..66580)
                     /locus_tag="PKL93_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNQ70169.1"
                     /translation="MILRSCPVFSPRPWGDRKLNRIYGVDSEEPIGEVWLLSDIEGMR
                     TWLESGSTRLSPNEVIDKLSGRSLPRFPIMVKHICASEWLSVQVHPDDKFARLYEGEP
                     WGKSECWYFLNEGQVLAGMKNKTKPVDSVREDDLNHILLKRGDLLALPAGIVHAIGPG
                     SELIEIQQNSDVTYRLYDWDRGRELHIEKASKAVNPSLMPGHYESVKRFDWEYFSVRE
                     TSRFSGTGVCVTIEEKPSLYLVIDDVMESAKPFLAITLGEFWSEIP"
     gene            complement(66580..67875)
                     /gene="trmFO"
                     /locus_tag="PKL93_02525"
     CDS             complement(66580..67875)
                     /gene="trmFO"
                     /locus_tag="PKL93_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNQ70170.1"
                     /translation="MIINIIGGGLAGSEAALSLADFGQEVNLFEMRPNVKTDVHQSGD
                     FAELVCSNSFKSESLDNASGLLKEEGLRLGSRLLEIAYHNRVPAGKALAVDRKAFSEE
                     VSMKLETHPLINIRREEVCSIDFDGAVNVVCTGPLTSNCFETFLGELFGGALFFFDAV
                     APIVAADSVDLERAFIADRYSDDGDYVNCSLSREEYELFWNELVHAEVLEVENFSDRF
                     LFERCQPFEEIARSGIDALRFGPMKPVGLIDPITGKEPYAVVQLRKENLSGSLLGIVG
                     FQTRLKWREQKRILSLIPALRNVDIIRYGVMHRNTFLDSPRLLNPDLQSRNHKGLFFA
                     GQISGLEGYVEAIVSGRLAAINADRYARGKQTVIPPVGTMIGGLINHITVSGRSPLKP
                     VYANFGLLPEIRMKNRREKNRKKVVVAQERIQRFLEVII"
     gene            complement(67872..68765)
                     /locus_tag="PKL93_02530"
     CDS             complement(67872..68765)
                     /locus_tag="PKL93_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNQ70171.1"
                     /translation="MIEFSFRPEKPIDLDSTLDCGQTFRWVKDGEWWKGVVRDTVLFI
                     KKSLGEITVKSSSNSLLGREISTGIIHYLGLEDDLDEIHSTLRRRLSKFDEEARFISE
                     KAISEANGLRILRQDPFEMVVEYILSTRNSIPMIRWMSDSLSAAFPENRVDFCGETFY
                     KFPTLGQAKLISEELLTNLKIGFRVPWLMKLFSRIDQESYFSQLIPLTVEEKLEELTR
                     HDGIGYKVGSCVVLFAYGELNAFPVDIWISRVMKDIYGIEGSTKKIMQFGMNSFFPYG
                     GYYQEALFRYYRTHKLGRNLN"
     gene            complement(68762..69067)
                     /locus_tag="PKL93_02535"
     CDS             complement(68762..69067)
                     /locus_tag="PKL93_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70172.1"
                     /translation="MIALAKRIKELGEECGMTFRLVDENGIPYDGDIDFEIPSLILAL
                     SRATGSRSSTVVEGVQITALHMDNLKEKCYLIVVGEFLEDNSYRLLKTIVESYEVEL"
     gene            complement(69064..69840)
                     /locus_tag="PKL93_02540"
     CDS             complement(69064..69840)
                     /locus_tag="PKL93_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNQ70173.1"
                     /translation="MLHVCCAPDLVSTVFKMEELRDSKLFFYNPNIFPEEEFFRRYKA
                     FKEVCESLSVVCPEPSYYPEDFSKILDSYVDEKEGGIRCSKCIELRLKKTAEMAKSIG
                     AESFSTTLLASPRKSVKLITLIGDKIANDLSIEFISGNFRVDRGKLNTLIRGIYRQDY
                     CGCKASLSEKKKEREIRDTRDRERLERDFGDLVNLWRFRGEVVLRSEIPVNDISELKK
                     LIEIIKPATLFDDLEDAELKNKRWLKTGTYNCRILKEKDQ"
     gene            complement(69843..70613)
                     /locus_tag="PKL93_02545"
     CDS             complement(69843..70613)
                     /locus_tag="PKL93_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNQ70174.1"
                     /translation="MRKLTVKELQIIGSVVILVLMGFVAVIGYTGTESTEQNFDHTES
                     VSFPIDLNSCTERELELLPGIGPAKARAIIDYRTSVGQFGSVEDILQVRGIGEKTLSS
                     FRKMVTVAGSATTPADVNTLIDINKADVLSLQDIPGIGEAKAKAIVEFREKNGSFRTS
                     DDLLKVPGIGPSILSEILASIVPLAGSEKQSGSLKVNVNTADEEALCGLPGVGPVIAK
                     RIVEFRARFGPFRSYEDLERVSGIGEKTVANLKELIEF"
     gene            complement(70610..71350)
                     /locus_tag="PKL93_02550"
     CDS             complement(70610..71350)
                     /locus_tag="PKL93_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNQ70175.1"
                     /translation="MSEESHDYVLKTLIVVVLGLSAIVFILGGYAILLREENRRAKLV
                     IEEIRSSVFTPSTDQPEVSENSVEIPTGTQIIREAISVPETQNFFLETFDYRRLITNS
                     FDFLAEGSEFGYVVVEEGTAFKLCVEKGLGKYFITRVDDQFGVMFLGENPLTGLYDSK
                     TAYGIQLVSHTVSDGIAPQVMDLRSAGYPAFVYKSITTDGRTFYAAILGVFPDANAAR
                     NYSSEVDVQSLQRITGWNITDRFPRQLK"
     gene            complement(71347..72336)
                     /locus_tag="PKL93_02555"
     CDS             complement(71347..72336)
                     /locus_tag="PKL93_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNQ70176.1"
                     /translation="MKRITFTSESTERCQLIFGKNMIEKLPESIRVVDSGFKKSFAGN
                     LKPEYLMPGGEHIKSIDRVFEIYQLVRVSRCKAVTAIGGGSIIDLVGYACAGHTEVEK
                     LFLFPTTTVGQIMPAINGFKLNFEFVKDLLCANGIPDRVFIDSSISYKEYLDSSRSDL
                     LFPLLVALSFDMRLFKYISNLVASGKEITEEQWDDIVFSSVKAYLKGIELKKPFVGAE
                     TGGLIETASRLRTGNRAAVILGAMVELRLAREFSNNDVKSSEVFFGLVRQLWNRNWPS
                     RIDLSSLVDLIEQKGGLKISTPDGSLDGTYFVGYSSFERFIREKTWRGLESFA"
     gene            complement(72333..73343)
                     /locus_tag="PKL93_02560"
     CDS             complement(72333..73343)
                     /locus_tag="PKL93_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ70177.1"
                     /translation="MKKLIEKLVTVSSPSGRESAIREVIVEEIKPYVDEMKIDPLGNL
                     IAVKRGSSGKKLLFDGHMDEIGVVVTHIDSSGFLRVDSVGGLSPYMLLGSRLIFETGA
                     SGVVDVEGETAKELGETIKNLDYDHVFVDIGAIDKKDAENKAPIGTFGTYDSTFVDLG
                     KRIVSKSMDDRIACAIMIQVLKELDKPKDDVYFVFAVQEEVGIVGASVAAFDIMPDMA
                     VAIDVTAGPDTPKSFKRMGFKLGGGPTIKIKDRGSVSSARVVSRLKEVAEKKEIPTQY
                     EVLIFGGTDARGYQTTGRGIASGTVSIPCRYVHSPHEMVDYDDVLNAVRLLKGLAEEG
                     VE"
     gene            complement(73340..74380)
                     /locus_tag="PKL93_02565"
     CDS             complement(73340..74380)
                     /locus_tag="PKL93_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ70178.1"
                     /translation="MKLKRLKELCELPGISGFEERVSSYIMEVVKSKVDSIWTDTVGN
                     LIAIRKGNGKSSKRLMLLAHTDEVGLMVKKINEDGTLSFTNIGGVDPRVLIGKKVLVG
                     EDLTPGVIGFEAIHTQDPSSVLKTPSMNKLKIYLGYSKKEEASKKHRIGDPVSFDTRY
                     EEIGDYAVAKAFDDRTGCEVLIRVLESLDEKTVDFDVYFAWVVQEEVGLRGSGVAAKQ
                     IKPDVALVFENTTAGDNPELPEYRWATSLGKGPVLTFAHSGLVLDKKILDTIVETAKS
                     NSIEFQYKSRIAGGTDAARLAKVMAGVPSGVISTPSRYIHSPTSVINVNDFLKVVELA
                     TILVKEGKVLSR"
     gene            complement(74377..75390)
                     /locus_tag="PKL93_02570"
     CDS             complement(74377..75390)
                     /locus_tag="PKL93_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ70179.1"
                     /translation="MNLARLLVDLSNAFGPVGYESEVHSVIKEAIDPFVDEIYTDEIG
                     NLIAVKKGDKKRIGVFTHVDEVSLVISKIDERGFARFEELGGIDPKVLISQKVKIKCR
                     DGEERAGVIGMLAPHLQKKETRGEVPSFDELFIDASINPDFEKIDVGDLAVVDFSAFE
                     MNGKVSGKALDDRACAAISIETARELSKYSLTPTVYFVFTTREEVGAIGAKGAAEALE
                     LDLGVAMDVTHHDKESDIELGKGPALTVGGPNIHKKYFELLNNYAKDNEIKVQYEFGS
                     GRTGTDADNVQIAGIGIPTLLLSLPEMHMHTPVEVVQISDVAGTARLLAGFFVSLKGD
                     EEI"
     gene            complement(75387..76064)
                     /gene="minC"
                     /locus_tag="PKL93_02575"
     CDS             complement(75387..76064)
                     /gene="minC"
                     /locus_tag="PKL93_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNQ70180.1"
                     /translation="MKKGVITVPIDFRMTKKGLILLIDSYRSVDELRQDIMKKFVDAK
                     DFFSEGDEISLMLTQDTSKPDDIVKIVSLLSDLGVHVKDILVGTMEQKDVKVGQKYDL
                     VREKITEVRGAQIIRRNLRSGQIVVHNFDVVVIGNVHPGAEIIAGGSVVIFGSAKGVL
                     RAGYSQGETGVIAAIDLQPSLIQIGNFITQEYDHFDGPAVAHVRTGRIIVEEADNVKF
                     EVKGEAN"
     gene            76195..76270
                     /locus_tag="PKL93_02580"
     tRNA            76195..76270
                     /locus_tag="PKL93_02580"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76229..76231,aa:Glu,seq:ttc)
     gene            76276..76350
                     /locus_tag="PKL93_02585"
     tRNA            76276..76350
                     /locus_tag="PKL93_02585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76308..76310,aa:Val,seq:tac)
     gene            76358..76433
                     /locus_tag="PKL93_02590"
     tRNA            76358..76433
                     /locus_tag="PKL93_02590"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76391..76393,aa:Phe,seq:gaa)
     gene            76547..77059
                     /locus_tag="PKL93_02595"
     CDS             76547..77059
                     /locus_tag="PKL93_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HNQ70181.1"
                     /translation="MIEKVQRHKYIMNLLEGSSVPVKGQFLADETGVSRQMIVKDIDE
                     LRKNGSSIVATTRGYLLGKRRTFRMVVSVKHDEEEIYDELWEIIKAGGHVVDVSVIHA
                     VYGELKGKLDLSTIDDLNRFIAALKASNAVPLLSLSKDGVHLHTIEADSHESLERIRS
                     ILKRKGYLLT"
     gene            77071..78348
                     /locus_tag="PKL93_02600"
     CDS             77071..78348
                     /locus_tag="PKL93_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ70182.1"
                     /translation="MNIDEVIQKYITKGRRTRMLLLTSLEWMLVAGGVMITSLTLPSI
                     VEDLAGTTGSQNMMASSVFVGMLLGALFSGMISDRFGRKWTNLILLLIAGLSTGFTGA
                     SVSMRAFSIGRFVAGIGYGGLLPVVNAYLTEFSSIKIRGLYLALLESSWAIGSIITGG
                     FTMLTLNSLGWRWSFYFLALFSVPLLLISFFLPESPKYQFMKKGKVALEKILGKEIDE
                     EIEMHKQSKQPILGLFKGDLARRTIMIWISWFSVSFVYYGIYTWAPKIFMSKGLTPVS
                     SLWYTFFMLIMQLPGYLTAVILIERIGRKPSLTFFFGGMAVSSIVMALVNSSALLLVA
                     SVLISLFVLGAWGMVYAYTPELYPTEMRALGNGASGVMARAAGIVAPFFTNLVMGGTG
                     SVFVVMIFMAALSLFAAIIVSKMGVETKQAIIE"
     gene            79262..80176
                     /locus_tag="PKL93_02605"
     CDS             79262..80176
                     /locus_tag="PKL93_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ70183.1"
                     /translation="MKIVRTLFLILLIVIAFAVVNGIFSVTVARVFLNRIEREGRYLK
                     VGEEELFLKVLGEGKPMLMIHGFPGSHLDFSELAKLLSSNRKVYLVDLPGSGLSEASS
                     KGDYSRKGYAELLVDLMNLLNIEKADIVGHSLGGEIALNIGYYYSERVENLILIDSYA
                     LTEQKLVPDFISSSKVLTWIAMRFYYQTYPVQRYLYTKRLGDKSYFVHEEFGKYFALV
                     DRMSIQFISEFIKDSDGGSLSGMLGEIDLNVLIIWGERDEILPLDYAKKISEEISGST
                     LKIIEGRGHAPFTDKPERVAEEILSFLE"
     gene            80193..81161
                     /locus_tag="PKL93_02610"
     CDS             80193..81161
                     /locus_tag="PKL93_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1722 domain-containing protein"
                     /protein_id="HNQ70184.1"
                     /translation="MIHSRPQVVVSRCLGFDTCRYNGQMIPSKFVKRLKKYVQYFTVC
                     PEFDIGLGAPRAPVRMVRKSDGIRLIQPLTGNDVTERLTDYSHRFLDGLSGVDGFILK
                     SASPTCGIKDVKVYPGEGKVMSSGRDSGFFGGIVIDRFNFLPVEDDGRLSNFIIREHF
                     LTTLFTVSRFRELSNNLTRKGLVDFQSDNKLLFMSYNQNEMRIMGAIVGKMDSRDIRE
                     VYDEYSSHFYKTMSTPPRHTSHINVLMHGLGYFSKYISSSEKRFFLDTLERYREGKIP
                     LSVPLYILRSYVVAFESEYLARQTFFEPYPEDLVEITDSGKGRSGK"
     gene            81158..82507
                     /locus_tag="PKL93_02615"
     CDS             81158..82507
                     /locus_tag="PKL93_02615"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNQ70185.1"
                     /translation="MRADRAHKLNKSSDRRGNLVLYWMQASQRTEENHALELAKQKAK
                     ELDVPLAVLFVLNANYPSGNLRSFSFMFDGLRDVKSEFEEQGIRFVIFKGDPVDIVPK
                     VSKRCAMVVTDVGYLRHQIDWRKRIAEKLECPFLSVESNVVVPVRTASCKEEYSAATF
                     RKKVMKHFAEFVSDFESVELSFRASLAEFEGATGIEDVDLSDLDQSVSIISGFRGGQK
                     EAKRKLEIFLSKNLDRFEEERNDPNSGCLSEMSPFLHFGHISPSYIARRVIDSCSRGK
                     NAYIEELVVRRELSMNFVFYNSKYDSFEALPQWAQKTLNEHRKDKRVRSFSLFELESG
                     KTDDPYWNAAQKEMTLTGKMHGYMRMYWGKKLLEWSTDPESAISSAIYLNDKYELDGR
                     DPNGYAGILWCFGKHDRAWSEREIYGKVRYMNSNGLKRKFDIDSYVKRIDNLSFDRK"
     gene            complement(82490..83296)
                     /locus_tag="PKL93_02620"
     CDS             complement(82490..83296)
                     /locus_tag="PKL93_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HNQ70186.1"
                     /translation="MKKLIIAFIVIVLLSPFLVAFQVDYTELSSYEPLVLDESTGTRT
                     KSVDFSVLSASDSNKVVIIASGWLFDPESEEQEREASIQLKVNGECYEKIVSLARQGL
                     YFVMKPFLLSFERGYSLSVMELVLDYESVLEDAVKKLEYECLALEIERSIESGISVGV
                     VEEKGFRETSVISGEPVLIIAAGEKPTGGYEIAVTSVLLTEARTIKVYATLHSPGRGE
                     FVTQAFTYPRKAIKILGLVPGIYDLILELEVMKDEKVIDTEVFSSSLSVE"
     gene            83486..84763
                     /locus_tag="PKL93_02625"
     CDS             83486..84763
                     /locus_tag="PKL93_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ70187.1"
                     /translation="MNRRLVFVLLMVLLSLSILIANTIELEFWTHEDPNRTPLEERFI
                     EEFQEMYPNVVIKRVTQSSTKIQELILTAFAANQGPDIFNMSIEDEFAYIANGRVAPV
                     DYEAAGYKDMEDLLASFLPGTLDPVKYEGEIYGLPLEITNWCILLNKKVFRDAGLDPE
                     IDYPKTWEEMMEVSEKLTIREGEIITRRGFDFRYPYYLVAFMPLVEQMGGKLVSDDGK
                     TAIINDEAWLNFLDYMAEWGPNGRNLGSPTYQNARKLFNMDNNDIGMCTTGLYQLARI
                     KADNPEFYDSGEFMVVPFPQFEDAVNYVPAHYYGHYYMVNSQKPKENQEMAWKFISYM
                     LSHAEEYLEKVAIIIPTNDLLESETFKNYPYSDVFISDLEKSTVVYFSESSAKIQSLI
                     KEAVESVMLTGTSSQDALNTLRRKVQEVLDDQY"
     gene            84820..85731
                     /locus_tag="PKL93_02630"
     CDS             84820..85731
                     /locus_tag="PKL93_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70188.1"
                     /translation="MRRVFNRGIEHKKARWGWFFVIPSLAFFAVFSFYPIMNAFYTSL
                     FRKRLLDLRPPKFIGFANYEYLLKSPDFWNSVKATSIFTLGTFIPIVVFSLLLAVFIM
                     SRRRFQKFFQIVYYSPAVLSSVVAAAIWLLIFDPRGLANTTMNTLLGTVGKDYKWLAT
                     AGMVRLSTILVYFWKYIGYFTIIYITGLAAIPQSLHEAAQIDGANGWKDFWHITLPLL
                     KPTTVLVSIMSMLQCLKTFSTQYLFTQSGAPTQPINVITLNIYNTAIRDHDIGRASAM
                     SIILFGIMLFFTWLQFRVSKSGGEVDY"
     gene            85742..86593
                     /locus_tag="PKL93_02635"
     CDS             85742..86593
                     /locus_tag="PKL93_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70189.1"
                     /translation="MKTIRKQSVVVDVLIWAFLIVTAIVILAPIVFMFTASLMPSKDI
                     LKMPYSWIPKDLYLDNYWQAIRGNDGSFIYPRNILNSFIVAGAVSLSTVFLSALTGYG
                     LAKFPFKGRNLVFLLIMATMMIPFEAIMIPLYLVATSLKIQDSYVGLILPFMVNAFGI
                     FLMRQYLITFPDEIIDAARIDGSGEFSIFWKIILPNSAPAVATLAILTFRSQWDNLLW
                     PLLISQSEEMKTIPLYIVKFAMEKHTNEGAMMAAAAIASIPMLILFLALSKYFVSGAS
                     LHSSRKG"
     gene            complement(86572..87540)
                     /locus_tag="PKL93_02640"
     CDS             complement(86572..87540)
                     /locus_tag="PKL93_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70190.1"
                     /translation="MRRVILLGIVFFFSSISLASGFYIGSGFNGLVEVIAGNQEWNIR
                     VIPVTSLSSGGIVAGKLIFNVLRDKGFAVIPVLQPGGYRVVDTFHCEAIEVNIERLEV
                     FVADGTHGVEVFKFYGSSFYKHIQTIPLKGWVNSLAYLGDYLLVGSELDGLFVIRRGE
                     KGFELIDHIYPRYVSDLEEGVVVNAITAWENSFYVAAGERGVMVFEIGDSALVKKAEI
                     KVGYAMDVSVSDGDLFVVDSRVDEILVFDTNNFSLKERHVVNGKLRETVPIIDWQNHN
                     KLLVCRLDDSVAVIDRNTSQEIFSLKGSFFGLVEARYFLDHPFLDE"
     gene            87692..88174
                     /locus_tag="PKL93_02645"
     CDS             87692..88174
                     /locus_tag="PKL93_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70191.1"
                     /translation="MSKKEFLKRILSEGRSADEIADLFGITAERLENYLEDPSRSKLA
                     VAEIKTELRRLESLPLETKEDILLFSFRVILESINFLFSDIESAASDKREERLPRLIR
                     KLIDGAYLLLAISRLEKVGKNLSSMRFDERFLRACQNKALVVDRLMDLAELIDREIER
                     "
     gene            88257..90296
                     /locus_tag="PKL93_02650"
     CDS             88257..90296
                     /locus_tag="PKL93_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70192.1"
                     /translation="MSDSRQREFISFLSAEDVIEKANESLEGKNWKPLAETSNAALIS
                     LKTPLVASFFKKFFETKYAFIYRSVEDELISSSSEKLSDLVDTLFQAATERQTLDDSF
                     FVFLDYFKEAPQILEKIVLHYSSLIADKSDSLLTSLRELKNLHERVRRRADQFGLNLA
                     LNEIVSRLFSLECPLLPTLESDLVDFLSKFPPDEEGASKFLDNQIVLAVGEYDESSVE
                     VQLSRLETIIKEIKRYSLTFMKWLTVPDNLFVSYLEKGFRRYGTIATWLEGIKEARTE
                     YGLGQSHWEQARRWYFEKISSRILPSFQKAMAGWACWTIIEERQIKDPLAHAGETMRY
                     SARRSEFRIIKCEESSGVFDLCSVDELMITSSGLKKGERSFPFNSIRTQLEELRLSRE
                     AFLDSKDAIFVGYTWKYLLPGGGPDKEAKNNERIECFNYYCVTVSATGFGVPLIKLFF
                     AREESARTFIEFLKEARIMSSWKEYPQNGLPAPLKAKTKNRSESEDQINPGEANHKTV
                     EELIIQNMVEESDESEIEETTEVPVVPISAELLEKINSLHYELEWSTKKMNEVEWTYF
                     FPEREKEEEDIKIAEPLQEEMTGFRELEAKIEALSGKTKRTVCKILSCEPEREYSLQE
                     LGLTFWSVFVVAPKVTSGGKPLIEVISGDVPRLVWGNNFDEETRELLKARFASDD"
     gene            90320..91513
                     /locus_tag="PKL93_02655"
     CDS             90320..91513
                     /locus_tag="PKL93_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HNQ70193.1"
                     /translation="MDYHPMFEAIRAGTAVPVDFVTEILTGREKYVQEIENDINYILT
                     GKSKVRVFLGEYGLGKTTLARYAEYVSREKGMMISCLSEKDYDTLHKQDEFFRSIMKN
                     MTMVGINGNPLKIFLRAWAEKTLGELDELRIPSDDVNSVREYLSTRDNYDPDGMFSHF
                     CAAYVASAGQGAPGEEFYAYLVGDSVDKRTMKKHGIYHFLQDDGWNFLKSFTSLMDTL
                     QVPGLVIIMDELESTMNRRRDVRDRTFNQLREIIDKMSAGFLQKTYFLWLGTDVWFEN
                     KHRGIASYHALYDRMKNISGDNSGKTIILNLEPLGKEDLRQLIRRLKTLYASCYSYTA
                     DSSFEENTLQSLVKQYTGIDGKLRPSVREVAKLTVEFLDSLRQGKGQEGNGDADSVKA
                     ADQLW"
     gene            91510..93633
                     /locus_tag="PKL93_02660"
     CDS             91510..93633
                     /locus_tag="PKL93_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ70194.1"
                     /translation="MIDPRLNQKLVDLFSYRYRWQSLREIQSKTIPPVLDGKNLLLIA
                     GTASGKTEAVMIPVVNRLLELSAGLKCIYFAPLKSLINDVASRLELILRPFGMVVGKW
                     HGDLTKSEKFSTAKEASILVTTPESVEGIFLSENIDLLSKIEFVIIDEVHAFIDSPRG
                     AQLASLMERIKILSGIDQQRIAMSATVGNPELLMEWLSGSSNRESEIVTDDSKSKRTI
                     EVLTEGEISPAEYLARLLEETDDKILLFSYSRAKAEDFAASAKALGLAIPVHHSSVSK
                     SLRVEIEEEFKRDKKLRAIVATSTLEMGIDIGDIDRVIFLEIPPSAASFLQRAGRAGR
                     KKNKSTISVFIEDPQSLYNLLGILDMLEEGSVELLKPSSFHLPLLGHQLIGLTKTAGS
                     LCKEDLTVLKRAFPFRQIGSADFEIVLDHLVKESFLARRGNAITSGAATAEMIGSGKE
                     KMDFVVLFPGGFEYSVIFNGHEIGKIHPAVLSTSSDDEVSFLLGGRSFLVKEVNPARK
                     TVHVVLGDSGKPPSWFGSSSLMTKEFARAIKSSLAETKIPDGVVLSQDASQTLLDFTD
                     NHRASSISISLRSSRKSVIIETFAGDMSNIFLSLCIKAVSGIKKISSSWHSVIVKDGI
                     DIDELHEMLLAISRMEENEIKGILSTYLRENPNELKKLYELLGEKLDRFVPQQLLIKY
                     VIHRFFDSLLLKELSEAIMVTDADS"
     gene            complement(93708..93980)
                     /locus_tag="PKL93_02665"
     CDS             complement(93708..93980)
                     /locus_tag="PKL93_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70195.1"
                     /translation="MQNIEKIIDLVEELFDELENADVQELTEALPDMEQDEVTELRSS
                     IEDWVDGLDEIIARLPELQASILELKGDLERLNDSVVEMEEDFEED"
     gene            complement(94231..95844)
                     /locus_tag="PKL93_02670"
     CDS             complement(94231..95844)
                     /locus_tag="PKL93_02670"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNQ70196.1"
                     /translation="MKKVLIILIVALISFASAAVACTILGVGKDAMVNGSTIITHNDD
                     STSADFRLWIIPAMDWPEGSMRDIVLDSHNYIDYGNYPEVNIGSKGTLIGQIPQVPHT
                     YQYFHSRYSFMNEKGVAVSESTFGIDTSTDYGKEVRKVLWTDSKGIIDCWTAQDIALE
                     RAATAREAVRIMGDLVEQYGWSTVGGGGETMTVADGEETWIVEFYGRDIWAGVRIPSD
                     HFTVAANRARIMEVDLEDTENVMASPNIVSFAVEQGWYDPSSGKPFNIAEIYAPNDSL
                     YATRREWRAFDLVAPSLGLSPHDLRFPLSVKPDRLLTVHDIFAIKGDYYQGTDYDLTV
                     GLAAGPWGDPLRYANTGKTGSWERSINMHRTCYVHIGETNSAYAEPFKGISWFGYGAP
                     DTSYIVPLWPIMRELPKFYETGSRYEEFRRDSGWWVNSYVQQMAELHYNLAIQDIRNY
                     RDPKLEVLYKVTPEVQKIATDMYETDPEAAIDFVSNYAFMNAVAWHEEWKLLGDRLLG
                     NYALGYVNFKSSPYPDWWNEAIGYGFPER"
     gene            complement(95964..97520)
                     /locus_tag="PKL93_02675"
     CDS             complement(95964..97520)
                     /locus_tag="PKL93_02675"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNQ70197.1"
                     /translation="MKLKLKAFLVLVILLVFLSSTALTCTILAVGKDASVDGSTIVTH
                     NDDSSSADFRLWIIPAIDWPEGSMRDIVIDSHNYIDYGDFPFVDVGDKGVVVGQIPQV
                     EHTYQYFHSRYSFIKEMGVAMGESTAGVRRELREARGWIDCWTAQDIALERASTAREA
                     VRIMGDLVEQYGWYGSGEIMNVTDGDEVWIAEFYGRDLWVAVRMPDDCVFVGANTMRI
                     RDVDFEDTENVMYSPNLISYAVEQGWYDPNSGEPFRPADVYAPRSSGNIREWRAFSLM
                     APSMGYEYLDVHYPLWIKPDKKLSVWDIFTISGDWYERTEFDLTKGIGAGPFGDPFAS
                     YRVGGRQRPIGIYLSCYVQISQIKNWLPPVIGSVVWFGYGGGGTQYHTPLWPAMSELP
                     EFYQVGSRYEDFRRDSGWWTSTYVQEMTRLRFKDAIVDLRNFRDPKMQAVYQTVPKIQ
                     ELATELYETDKDAALAIISDYAYNTAVAWQADWLKFGDYLVGKYCADRINFGTGLPKE
                     WGELLDSLNN"
     gene            complement(97580..98773)
                     /locus_tag="PKL93_02680"
     CDS             complement(97580..98773)
                     /locus_tag="PKL93_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNQ70198.1"
                     /translation="MKNPLYLKILFLVLSGVLGLLAGIEFWELRHYKETVVVSEDFSE
                     KIMLSQYLPSLKNTWGDTPIYIYDSGVPGGSMLVLGGTHPYEPATTLATYVMMENIDV
                     TSGKVYIIPHANMSASTLGMLGNAYIKYYHIETPWGQKKYRVGDRGTNPLDQWPDPFT
                     YVHYPSTQNLAYQDIRNLNRTFPGRPDGTLTERISYAIMELIRNEDIDIFFDFHEASL
                     MYPVVSTYVAHDRSMDIAMMAAMMLSATQFPMKCEASPKNLRGLTHREVGDFSNTLAI
                     LMETPEAFIDRVVGRMSEELMLEGKDEFLQTAAEKGLVYCDYDIYEGFPDALGNMIIG
                     TPMDYRVGRHLSGTLEALNWMNQFFPDKAISVTFPNYVDVMSNGSGYYLHDPSKADPS
                     RVFQN"
     gene            complement(98782..98958)
                     /locus_tag="PKL93_02685"
     CDS             complement(98782..98958)
                     /locus_tag="PKL93_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70199.1"
                     /translation="MLVLMDTIIHYFYFVMTGFFAILLLKNLFKRERRKGLVYDIVYS
                     YVIMVFILRVIGIK"
     gene            complement(98972..100273)
                     /locus_tag="PKL93_02690"
     CDS             complement(98972..100273)
                     /locus_tag="PKL93_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HNQ70200.1"
                     /translation="MYLQTTIIAIIMVATFALASWKLKSPEISMVITAIVGALVGGFG
                     FPSRLLVEGMFTYFDVGMIFLTASIFINIYSETGAMNALVRWIVKQFYDRKWLMLVLL
                     GILMIIPGALTGAGSVSIFVVGGLVASVMSYMGFSKRRVAAFIYLFAMLAAVSPPINL
                     WAMLMAAQANMPYVGFNVVLLVPIVIIALFTIVYTGTSKTPERKSKEHILSELPLPPE
                     GMNWFKIITPFAVLLVLILLSKFAAFSIPVLGLPLTFVICAFASAMMDRKRWDVRKWF
                     DVMVKTMDQVFPLLATVISVGALVNIMTATGVRGLLAITFITLPMVFIYITALLFAPF
                     AQGALSYGSAIILGTPIIFLFNSVGLNVTVTAAALSLLFPLGDCLPPSRIVGRLTVET
                     VGYEGSYMSFLKAIAIPWLFMGAIGLLMLIFPNQLDFLVVY"
     gene            complement(100315..100917)
                     /locus_tag="PKL93_02695"
     CDS             complement(100315..100917)
                     /locus_tag="PKL93_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNQ70201.1"
                     /translation="MRKVLIVVLLSMVLFTTGLFAVVSGDPVVEGQLPFLLTNAGQGP
                     GGKMARLLIWQSGAIEDTDFDYNAEPLKENPNDLTAREYKMLFVIIGSSAKGLGASGI
                     TIDEEIVRLNKMIEEAKRLNMFIIAAHIEGKARRGNPGSADERSIDAIAPYADYLIVM
                     KEGNLDGKFTRIAEEKDIPLTLIDNTIDFMQVIKLMFPAE"
     gene            complement(101424..102074)
                     /locus_tag="PKL93_02700"
     CDS             complement(101424..102074)
                     /locus_tag="PKL93_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HNQ70202.1"
                     /translation="MMDPKERIARRIGRELADGDLVNLGIGIPTLVSNYIPAGIRVFF
                     QSENGIIGMGPLPEEGMENRDLTNAGGQYVTALPGSAVFDSAMSFSIIRGGHLDVTVL
                     GGLQVDERGILANWMVPGKMVPGMGGAMDLVTGAKRVIVAMTHTSRGNPKIVKQCTLP
                     ITSNRRVDLIVTELAVIEPFAGGLLLKEKASEVTVDEVQSLTEARLFVAEDLKDMI"
     gene            complement(102071..102718)
                     /locus_tag="PKL93_02705"
     CDS             complement(102071..102718)
                     /locus_tag="PKL93_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNQ70203.1"
                     /translation="MGMKKIVSLIEKGSSVMIGGFLGCGSPDSIIDEIVKNEISGLTL
                     VANDTAFPETGIGKLIVNKCASKIVVSHIGTNPETQRQMIDGELEVELVPQGTLAERI
                     RAGGVGLGGVLTPTGVGTVVQDGKKVLTVDGRDYLMELPLRADFALVKAKKADYFGNL
                     VFSLTARNFNPLMVLACDTVLVEVEEIVPIGSLSPDEIHIPGVFVHYIVVGGRVQ"
     gene            complement(102912..104300)
                     /locus_tag="PKL93_02710"
     CDS             complement(102912..104300)
                     /locus_tag="PKL93_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ70204.1"
                     /translation="MSYSLFKSYLNDEEAKEIRGSLLVMAIPAMAENVLQMLLGISDT
                     AFLGHYDWRIMTAVGTANQVVFIFQAVLVAISTGSMVLLSNSYGANNHRRVDLIAWHA
                     IYLSIAAGLILSFGSFFSDSLLSVLFPSSDSLMQMNGSKYLQIIMAGFPAMSIMIVLG
                     AALRGAGDTKSPLIVAAVANVLNVFLDYSMIFGKFGFPEMGAFGAALATVLSRVVGSV
                     IIIVLLFRNRRISMSRKPRRFSKWLFKEIFVLGLPASIENFGFSLGVLVFANILFISG
                     PQAYAAHRIGIQVESLSFMPAWGMGVAITALVGIYNGGRQRRMSIGVVRQGWFIALAI
                     SSAIGLTITLLPDLFISLFTNEPSLIEEGRLPVRIIGLFQVVMGTDYVVTGALRGMGD
                     TSFPMKTSLVAMWFLRLPIGYVLVRYFGLGLFGAWIGMMADMAFRTTLKFIRFYSGKW
                     ESTADSIQARSG"
     gene            104695..105426
                     /locus_tag="PKL93_02715"
     CDS             104695..105426
                     /locus_tag="PKL93_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNQ70205.1"
                     /translation="MKPEDIESFLNSVLHPEEFDDYCFNGIQIEGSKEINKVVTGVSF
                     SDALVEKAVTAGADAILVHHGIFGKEFFRLSGYLKKRVERVLSLGITLFGYHLPLDAN
                     EPYGNNYSIAEEIGLVNLERYDVGFIGTHPENILVSELRNSLCRIFPRQEVGLFKNSD
                     FAGRVCIISGGSSSNLSELEGTADTFISGEVGEPTREIAKETGINFFWVGHYASERFG
                     VINLGELLRERFSLEVEFVEIYNEV"
     gene            complement(105411..105956)
                     /locus_tag="PKL93_02720"
     CDS             complement(105411..105956)
                     /locus_tag="PKL93_02720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNQ70206.1"
                     /translation="MLKGRKVYLREYRKEDIVLAHEYINDYEVKRMLVPGIPFPLRLE
                     EEEKWYDSQSSSKDTYSFAIAKIEDNEYIGGCGINEVDWKNSVVTVGIFIAKKFWNEG
                     YGTDAMRILLRFVFEQMNINKAKLNVYSFNRRAIRSYQKCGFRIEGTLRQEIFRDGSF
                     HDEIIMGILKDEFGRNDQTSL"
     gene            complement(106038..107345)
                     /locus_tag="PKL93_02725"
     CDS             complement(106038..107345)
                     /locus_tag="PKL93_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HNQ70207.1"
                     /translation="MSSENSAYQEVREQEDRSKNLGRGGLLVLGLQHAFTMFGATVLV
                     PYLTGVPVNVALFTAGIGTLLFHLLTKWKVPVFLGSSFAYIAPINAVILYHANVPEIY
                     ESVPEAIAAGFSITPDMIAYATGGILIAGLVQVAIAMLIKRLGVSMFEKIFPPAVAGT
                     IIAVIGLNLAPTAISMASSNWWIAIVSLGTAVFVRLYAKGFTRLIPVMCGIVVGYIVA
                     AITGNVTFDAVSQASWVGLPQFVLPKFSFYSLTVLVPVALAPTIEHFADIFAVSAVVG
                     KKYYEDPGIHRTLAGDGIATAVAGFFGGPANTTYSENTGVLAITKVFNPVVMRIAAVF
                     AIILSFVPKVGALIQSIPTAVMGGIEILLFGMIAAIGMKTLIENRVKVDGKNLIIISV
                     MLVVGIGGAAIGIGPVRFEGIGLAALVGLLLNGIFVMTKAPEE"
     gene            complement(107809..108252)
                     /locus_tag="PKL93_02730"
     CDS             complement(107809..108252)
                     /locus_tag="PKL93_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNQ70208.1"
                     /translation="MITVSDMLDIALRIESVGYSYYQKLSEKTSGEVKSLFVKLATEE
                     REHAAIFREMLKEAENSPTAQDWEDNVGYLKSYAEISIFPRIESIDVPNNINKAISSA
                     MEVEKDSIIFYSELSFFIPDCKELKDIIEEERRHLHDLVKLYGSI"
     gene            complement(108358..108633)
                     /locus_tag="PKL93_02735"
     CDS             complement(108358..108633)
                     /locus_tag="PKL93_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNQ70209.1"
                     /translation="MEILKVSSKSNPNKVAGAIAGVISKNEQVELQAIGAGAVNQAVK
                     AVAIASRFLREQGTKVYMVPGFVEIQIGEEMRTGITIKIVSEKDEIK"
     gene            complement(108752..109906)
                     /locus_tag="PKL93_02740"
     CDS             complement(108752..109906)
                     /locus_tag="PKL93_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNQ70210.1"
                     /translation="MREFVLLQGDEACALGAIKAGCRFFAGYPITPATEIAETMAREL
                     PKCGGTFIQMEDEIGSAAAIIGASLAGEKSMTATSGPGFSLMQEAIGYAAMTEAPAVI
                     VNVQRGGPSTGMPTKPAQGDIMQARWGTHGDHQIIAIYPSNVEEVYKYIITAFNYAEL
                     YRTPVIFLMDEILGHMRESVYLPYDSEIMEVQRVGEAEFAEEDIFHPFEGDDQMMATP
                     LVKMGKSRFHVSGLVHDESGFPVGSPSDSARLLRRLDNKIRLHSDEIVIYDEYMTEDA
                     EILVLAYGSVARSAIKAVKMAREDKINVGLFKPVTIWPFPSTRLRQLLKKVSAVIVAE
                     MNLGQILYEVSRLNKRGAKIEHVSKVSGELITPQEVLDAISRVKSEIMDL"
     gene            complement(109899..110126)
                     /locus_tag="PKL93_02745"
     CDS             complement(109899..110126)
                     /locus_tag="PKL93_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ70211.1"
                     /translation="MAVKKQQYKVNINLALCKSCGICYWICPTKTITKGELGRPKVVD
                     QKTCIGCLMCENACPDFAIDVWEVEAVKEDA"
     gene            complement(110149..111270)
                     /locus_tag="PKL93_02750"
     CDS             complement(110149..111270)
                     /locus_tag="PKL93_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNQ70212.1"
                     /translation="MSTLISGYMAQTGLATVSWENLVMFAIAGLLVYLAVAKDAEPLL
                     LVPIAFGMVIANIPPEATGVFTPGTGIMWILQQGLMLGIYPPLIFLGIGAVTDFSFVL
                     ANPKTLFLGAAAQIGIFITFLAANLMGFSLSDSAAISIIGGADGPTSIYVASILKSQF
                     LPIIAIASYSYIALIPVLQPPIMKLLTTHKERAITMGKKLRRVSKLERIVFPVVATIA
                     ISLIVPQALPLVGMLMLGNLLRENGKTKRLVEASGKYISDTVIILLCVSVGAKADGKI
                     FLTIESIMVLALGCAAFVVATASGILFAKLMNLFSANKVNPLIGAAGVSAVPTAARVA
                     QKIASEEDPTNFVLMHAMGPNIAGVIGSAVAAGVFLSII"
     gene            complement(111330..113972)
                     /gene="ppdK"
                     /locus_tag="PKL93_02755"
     CDS             complement(111330..113972)
                     /gene="ppdK"
                     /locus_tag="PKL93_02755"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNQ70213.1"
                     /translation="MSEKFVYYFGKKKAEGDAKMKNLLGGKGANLAEMVKIGIPVPPG
                     FTISAEVCKYYYEHGNKYPESLENEVKEAVRLLEEETGKGFGSSERPLLVSVRSGAAI
                     SMPGMMDTILNLGLNDETVNGMIKESGNPRFCYDAYRRFLQMFGDVVLKIEHSEFEDA
                     LSSIKKEKGVKLDTELDADDMKKVVKRYLEVYSRAGKEFPQDPWEQLWMAINAVFGSW
                     MNERAKKYRALNGIKEGELLGTAVNVVAMVFGNTGDDSGTGVAFTRDPNTGEKQFYGE
                     YLPNAQGEDVVAGTRTPFPLTKLNDINPAVYKQLLEIFEKLENHYRDMQDIEFTVEHG
                     TLYLLQTRNGKRTPYASVKIAVDLAEEEKISKEEAVMRVTPEHIETLLHPYFTKETME
                     SAEFLAKGIAASPGAASGAVAFDPDTAEKMVREEGKAVILVRPETSPEDIAGMAAAQG
                     ILTSTGGKTSHAAVVARGMGKTCIVGCEAVEVDQKTRTMRVNGKTINEGEWISIDGTS
                     GKVFMGKLDSVKPQGLEGPLAKLLGWADEIRKLGIRTNADTPRDAKIAREFGAEGIGL
                     TRTEHMFFQEDRIFAVRQMITSSTREQREKALNKLLPMQEEDFYGIFKAMEGLPVTIR
                     LLDPPLHEFLPKEDEDVKELAEEMGMTYEELKTTVEDLHEFNPMMGFRGCRLAVVYPE
                     IAEMQTKAIIGAALKLVEEGKSVVPEIMIPLIVELEELKFVKSVIVKAADELISKSGL
                     KLDYKVGTMIEVPRAALTADEIAKEAEFFSFGTNDLTQMTYGFSRDDYGKFVGHYMEK
                     GILENDPFQKLDRVGVGQLVKMGTEKGRSSRPDLKVGICGEHGGEPSSVEFCHIVGLN
                     YVSCSPYRVPVARLSAAQAVVKNR"
     gene            complement(114180..115064)
                     /locus_tag="PKL93_02760"
     CDS             complement(114180..115064)
                     /locus_tag="PKL93_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ70214.1"
                     /translation="MKRLFLFTLVVLMVTVLSFGQTRLNVGAKNFTEQYIVSSMISHL
                     LEDAGFRVTENFGMSSFVARSALETGQIDLYADYTGTAWPTYLGHEKMIRDPHELYEA
                     VKAEDLENGIVWLDMANFNNTYALAVRRDFAAEHGLTTLEDLAELTHENQDLLFGIVY
                     EFLERDDGFWPMSEAYGFAVEKRQVKTMEIGLTYEALDKNQIDVAMVFSTDGKLEKYN
                     LLVLEDTKSFFPSYNLAVTVRKEILDNNPEIEEILRPISVYLTEPIMIRLNYLVDAGG
                     FEPDEVAEKFLKGLGLID"
     gene            complement(115086..115727)
                     /locus_tag="PKL93_02765"
     CDS             complement(115086..115727)
                     /locus_tag="PKL93_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70215.1"
                     /translation="MEVLEYMFENSGYIMERLLEHIFIFLTSWSMAVVAGVAIAIFVT
                     RPGHRKAGKLVLSITGATQAVPSIAVIALVFLFMGIGAAPAVVALFLYSLVPVVFNTA
                     SGLMNVSMMMKEAAKGMGMTNRQILWKVEMPVTVPTMLSGIRSSATINIGTAAIAASI
                     GAGGLGEIIFTGLRMTRGPMIIAGAIPVTILAIAVDIILGFSEKALTSKGLRT"
     gene            complement(115732..116859)
                     /locus_tag="PKL93_02770"
     CDS             complement(115732..116859)
                     /locus_tag="PKL93_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730312.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HNQ70216.1"
                     /translation="MSIEFKNVVKEYEEGFKAVDNLSITFEDKELTILIGPSGCGKTT
                     TLKMINRLIEKTDGEIVVDGTSLDEIDPIRLRRSIGYVIQEIGLFPHMTVFDNIAVTP
                     RLMKWDETRIKKHVFELLDLVNLEPSDYAEKYPAQLSGGQRQRVGVARGLASDPKIVL
                     MDEPFGAIDPINREKLQDGFLEIQSKIEKTIIFVTHDIREAIKLGDKIAILDDGKLVQ
                     YADTMTVVQEPANEFVEDLLGADRALKGLELVRVRETYVPGGKWIEDTGDCKVKKAKD
                     FLIDEGRKFAYVVDGSKRLKGYVMLKQLNKASDEEPLKKYLRSIETIQPLSNLMEAMS
                     LIMNTGLSSLPVVDDKERLLGTLRFKDLVNLVGSYESSDEE"
     gene            complement(116872..117534)
                     /locus_tag="PKL93_02775"
     CDS             complement(116872..117534)
                     /locus_tag="PKL93_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70217.1"
                     /translation="MGFFEYLGRNYDLVLIELLNHIKIIAIAIPIAIGIGVPIGIVVS
                     RSKRASSVALYGAGILMTIPSLALFGYMVVLLAPLKAGIGVTPAVIALVIYSFLPVIR
                     NTVVAVNSVDPRMIEAARGMGMTNWQILFRVRLPLTIPIIMAGVRNAAVMGVSVATIA
                     YLIGARGLGYFIFSGLGRSNFNMVLLGALVVSILGIGTNYGLLALEEIITPRGLKIER
                     EK"
BASE COUNT        33912 a        26188 c        25548 g        32153 t
ORIGIN      
        1 aaatggtaaa ccctttgaat tctcaaaatc attaagagcg aaagatactt gcttagtctt
       61 accagacaga aactccttta gcctcagcgc acaaacctcg tcgagcatgt cagaaaatcg
      121 tttcatatcg aattcttcac tcaagaaact gagttcatcg aagcaagctg cggcaagcct
      181 ttcccatgtt gaatttgcaa ctacgatctt cccttctgaa gagacaattg cgcacgcttc
      241 gacgtttcct tcgagaagag ctcgagcaat gtacaaacct tcgtcaattc ctctctttgc
      301 agctctcatg acgcacctcg catattgtat gtctgacaat tcatatgcaa atacatgcca
      361 actatgattt tgaataatcg ttttttagag tatagaattt acccgtgaag aaggaaaaga
      421 ttgaatacag acaatccgag tcgagcgact tgcaatttgt agcgcatctg gtcttcaata
      481 ctgacccatc ccatttcaag cgtgcactgg gaagaaaagc tctggcaggc atatctaggc
      541 ttctgattat gggcagcgcg ataaatctcg aattcattac tgtttgcgaa attggcaatc
      601 ggccaatcgc aatatatgca ttttacacgc atgaggagat gaacagactg tcgcatttac
      661 acgttgacat gatcaacttt tcttttagta tctttgggtt tagactcttg catgaatttc
      721 gaactcttca aagatcaatg gagatgggca ttttccccga gcaaaaatac ctcggtctaa
      781 tctcagttca cgaagatttc agaaacaagg gatatggacg gaggatcctt aatcatctac
      841 tcgaaatgga tgcagaaatc gttttggacg tctctcgatg gaacagaaag gcactatctc
      901 tatactccga atgcggtttc gaatttgaaa ccaatcttcc ttccaactat ccaacaaagg
      961 acagcatacg aatgaaatcc actcgcgatc atcactgact gaggtgttgc taagctctca
     1021 tcaccaccct taagagtctg tcagagaaga aagagcaact tcctaagaac ttcgtaataa
     1081 tcttgatctc tgaaagtgag aacacgatcg tccttcttgg tcaccccggg gaaaaaagat
     1141 aacttatgag ctaaagtgtg ttctatgtag gcaatttcaa cctcaaattc agccggaatt
     1201 gataccggtt gaactccgat attcttcaca gcattgtaaa caccttcctc gatcaacttc
     1261 actgccttag cgggatgaat tgaatctact gaagcaccac tgccaatgtt ggttgacacg
     1321 gtttgtatgc caggtacgtt ttgcttagcg atttgtacaa ttccctcatc cccgctgatg
     1381 aaaacgaccg gtaccccatg ataagaggcc gacatcgcgt ttagctcaaa ttcgctaata
     1441 agcttgttgt tgatcttgag gtagtatatg tcccccgtca tagtatgaga aagaggattt
     1501 agagcctttc cagagggtga atggtatcct gtgaacacca cggctccaaa actagaatca
     1561 attccttgca tcatcatgaa aggatctcta gcccacccac ggagaactcg aactccttcc
     1621 ggcagaagag agggatcgat gtttcttgcg ctcccgtggg catccctcac agttatttcc
     1681 gaggctccag cctcaagagc accctgaaca gctgccttta cttctgcagt catctgtttc
     1741 ttgaaatact cgtagtcctg attacccttt tttgcttcat tccagtccgc aattcctgca
     1801 gttccctcaa tgtctgcact aatgaaaact ttcaaagata tccctcctgt ttagttttct
     1861 ataaaatgca agaagttttc ggtaaaagta gaaaaccctg aaacaccact ggctggttta
     1921 ctgaatgacc aaatattggc attaaggaaa taaacaatga aggatcgaga gcatttttct
     1981 atttattcct ccaagttgac caagttgcca gaaaaaatcg atcagcattc catcctcaaa
     2041 agattctatc cacaatcttc aactgctttt tttgctagtt tatccgcaat ctcgttgaat
     2101 gtctctccaa tatgtgatct tgcttttacc cattcaatat cgatctggga ggagaactgc
     2161 ttgagccgtt caacgtactt tctcgttaat ttgtacttcg ctttccactc cccctccgcc
     2221 caacttctta gtcccatgta gtcatagtga atagtagctt ttctatatcc ctttcgcata
     2281 cagtattcca gcgccctaat cacgccttcg atttcacctg taacgttttt catttcgaga
     2341 ttctctctta aacacccgtt gattcttttc tcaatttgat tttctgtgat caccaatact
     2401 ccatatccta ccttattgtc catgaaacta ccatctacga agaggtgaac tccatcataa
     2461 tttcttccgt tgaattcctc ccatctaaac aggagccatt cgctcttctc ttcggagaga
     2521 aatgaaggca gaaccattct cttgtttcct ttacggttcg tataaagcct gagcactaca
     2581 ctgtttgaaa gttcaacgca gtactcactg atgacagtga cagaacagca aaggcctctt
     2641 ttcttgagga atctcgcgaa ttcgtaaccg gtttctttca aagcaagcgt ccagtcattc
     2701 aaagtcatac tctatacagc gcctcgataa aatccagaaa aaggattgtc gtatacccgc
     2761 cgactgcaat gaaagggccg aaaggaagct gagctcgtcg atcaaatttt ctgccttttg
     2821 cggcagcatt tattgcatag acaattagcg ctgcaaaaga agctaccatg atcgtatata
     2881 aagaacctat aatgccagat gccagagaaa gtgctgcaag caattctacg tcgccaaaac
     2941 caaaactgtc tttccttaca aggtttgcta tgaagaaaaa tgcaaccatc gccccagtaa
     3001 cgagaagagc atgaagtaaa tttgcaggga atcgaccctc aacagttgac caggcgaagc
     3061 cgccagcagc cacaagaatc acgccgaagt ctggaatcat gtagtgctca agatcgataa
     3121 aagaagagac aatcaatccc atagtcaaaa ctgacattga aattgctcca aacagagaaa
     3181 agacaactga attcaatgag aacaacagtg cagtaagtaa ctcaacgacg ggataccttg
     3241 cagcaatggg ttccccacaa tatctacatt tgcctcccag aaagatgtag cttatcaaag
     3301 gaatattgtc cttccatgat agctggtgtt tgcattttgg acaaatactt cttcgaggtc
     3361 tggtcattga gtattctttt gaaggaatac gaaatatcaa agcatttgaa aagcttccaa
     3421 atatcagccc aaaaatgaag aagagagaga ctctaagaac tatgtaatcc cacactgttt
     3481 tctctcttct tcagtgaggt attcatcgaa tagctctttc ttcccataga agtaccaaac
     3541 ctgctcattt tcgaattctt taaggagtct ctttgcctca tcgcactgcc ctttcttcat
     3601 atgagaaacg actagcagca tcttaagctc tggcctatcg gggttttcct tgagaaaatt
     3661 tgtcgccact tcttccacaa cgtcgtaatt tgagtgctta aactggattt ttgcccaagg
     3721 ctccacatca tttatgtaaa ccggttttgt tctaagcacc tcatcttcaa caattctccg
     3781 catctctatg tttcccatct tttcgtaaag ttcggccatt tccacctttg taagaatact
     3841 gtctggatct cttttcgaaa ggtctttgag aacattcaga gcttcatcca ttcgaccggc
     3901 attaatatag gcatcagtta acatgaagta ggtgacaagg tgatcgggag aagctgcaat
     3961 ctccctttgc caacaatctg ccgcactatc aaaatcacct ctgttataga atgtctgacc
     4021 aagaaatgaa tacgctgtaa caagtgaaca atctatcttt agagcatttt gaagccattt
     4081 aattccctct tcttcatcac ctgactctag aaaaatagag cctttcagct cacaaaccgt
     4141 cgctttgtca acatttgcat ccatgagcaa atcaagaaac ttctgggcgc tttcatagtc
     4201 accccgatca gctatagaga gtattttgtc ctgaaacccc gaaaatgtac ctctagtaaa
     4261 agaagccaca gagagacctg tctctttcaa gtaatcattg aattcctcgt attctatggg
     4321 agcgtagttt tcgggagtta caacattgtc gaaagtttcc gctaaaccac ctattgtcag
     4381 aagatccttc ccaattttag tagacttcaa atcatgtatc gcaacgttta tatcaccatc
     4441 gtaaacctca tcgaggattg cgatcgcaaa tggtagtgtg tccgtgatat caaagctctt
     4501 cttgtctttc ttgaggcgaa taatctccat atagcacctc ttctcagagt gacttgtaaa
     4561 aaatatacca tgtttgctcg ccgaagaagt aattttacct aagattggtg aatctttcta
     4621 ttcttgtttc accatgaatg aggtctgccg agatcagaat gaacagagac cattccaggt
     4681 agaaaaccaa aatcacttta ccatgaagga cttcaaaata ccggtgcact gcgcttctta
     4741 cataaggcaa ctaattatta agagcttgat agcttgtcaa cgtgatttct tatggctcct
     4801 tgagtttttt cccttcgact tatcgggaga ggagcgctta cttctcctgg tcgtctttcc
     4861 gttatcattg acaataatga aatcgatttc catggttgtc ttgtctgctc ttacaacctt
     4921 cacctcaagc tgatctccga tcctaaatac ccgcttgctc cttcttccca caagaatatt
     4981 ctggtcctca atgaagtcat aatagtcatc cagtgttgaa atgtggacta gaccagaaat
     5041 cattttttcg gtcaactcca caaagattcc gaattttgtt acacccgtga taacaccttc
     5101 aaaagaatca cccacatggt tggagatgta ctccaccttt ttcatgtcca caagatccca
     5161 ctccgcttcg tttgcaattc tctctctctt cgatgcccac gaggcaattt tggaaagact
     5221 ttcctccaga ttctttgaat ggttcttaag atcagccccg gcattcttga tcaaatcttt
     5281 taacagcctg tgaacaacaa gatccgggta ccttctgatt ggagaagtga agtgagtgta
     5341 tgcttcagat gccagtccaa agtgaccgat atttctgtca gaatagacag ctcgcttcat
     5401 tcctctcact agaactcttt ctatggagga gtggaggggg tgatctttag acttctcaag
     5461 tatttgctga agagccttag gatgaacgtt tctcggaatc ttgactttca atccaagtgc
     5521 ttccagataa ttttttagtt gcatcattga ttctggatct ggtttatcgt gaattctgta
     5581 cacaaacggt aacccctgat tgttaaatat ctcagcaaca gtctcatttg caagtatcat
     5641 gaactcctcg attatccatt cggatattcc tctactctgt ggcagaatat cagttaccct
     5701 gcctttctcg tcaaagatga tctttacctc tctagatgaa atatccataa ctgatccccg
     5761 cttcttgcgg atgtcccgta tcttctgagc caattcgtgc gacaatttga gactctcaga
     5821 aactggtctc aatttctccg aaatatctga agatgcttta ccatttagga aatcgttaac
     5881 ctctgaatat gttagtctct tcttagagtt aataacggaa ttcttcactg acgaggaaac
     5941 cacacgtcca acagggtcaa tctccagcag cagcgaaaga gtaagtctgt cctcttcagg
     6001 tttaagagaa catatatcat ttgaaagttc aaatggcagc atcggaataa ccgtatcgag
     6061 caaataaaca gaagtccctc tagaataagc ttccaggtct agctgagtcc cctcttgtac
     6121 ataatgcgac acatccgcaa tgtgtacacc aagaatgtaa tttccttctc tgctctttct
     6181 tatcgatatt gcatcatcga aatcttttgc atcatcaccg tctattgtga aaaccaattc
     6241 atttcgaagg tcttctcttt ttctaacttc ttgaagatca atgctctttg caaccctggc
     6301 tgcttgtttt ctcacctgct caggaaagta acccggttct ggaaggtctt gtctgtaaat
     6361 aactgtcatc aaatctgctt cgggactgaa cacgtcacca ataacccttt ctacgacacc
     6421 ttcaggattt ctccccgggg aaggatatct ggtgatcttg attagaacct tttcgtcggg
     6481 tttggctcca ttgaatcctt cgggagggat atagaagtca gtctttatcc tcatatcatc
     6541 agcgacgaca aaagccatga tgcccctggt cttgaatgta ccgactactt tctttttatt
     6601 cctactgatt actttgatga ccttcccctt tctaatttct cgatatcttc ccgttatttc
     6661 cacaagaaca gtatctccat gaatggaaaa accactgtct tcgggagcga caaaaacatc
     6721 ctcttcgtct tcaacgctta tataagctcc cgtccctctt cttgcgaatt caatagtacc
     6781 aactatagtc ttttcaccag gaacaatgta ccttcctttt aaatccttta taatctcgcc
     6841 agaatcggtc aattcttcga gatacttctt gagcaacgcc ctatcctccc gatcagttag
     6901 gccgagcttc actatgagac ctctcattgt cgtaggtgaa taatccttgg aggtcatttc
     6961 ttctttcaat gctttcaaac ttagcttcat aagcctcttt cctctctgtc atcagacaat
     7021 tttaggactc ttcatgttat aattatacag tgtatgtatt ataaccattt atggcttaca
     7081 ggaggtcggt aatgcgaatt ggaatattga cgggcggcgg ggactgtcct ggtttgaatg
     7141 cagttattag aggcatcgtg aaatctgctc cggaaaacgt ggaaattatc ggtttgatgc
     7201 acggttggaa gggattggtt gttggtgaaa gtatgaaact caccgataac gatgttgaag
     7261 gtattcacat tctcggaggt acaatccttg gaacatctag aacaaatcct tttaaatcgg
     7321 aagatatgaa aataaaaatg gaggaaaaca tcaagaaact cggtcttgat gtgattatag
     7381 gaatcggagg cgacgatact ttgaccgtgg ctgcaagact ctcatccatg ggatacaaag
     7441 caattggagt cccaaagacc attgacaacg atgttgccaa caccgattat acttttggtt
     7501 atcaaactgc ggtaaatgtt ggggcggacg cgattgacag attgcactct actgcaaagt
     7561 cacatggacg gatcattatc gttgaattaa tggggagaga agctggttgg gtaactttgg
     7621 aagctggcat ggctgccggc gcacatctaa tccttattcc tgaataccca atgacaatag
     7681 atgaaatcct aacctatgtc aagaagcgaa tggatagatt cggatacatg attatcgcag
     7741 ttgcagaggg cttcaaacca actgaattgg aaaatgttgt tggcgatgaa agcaagatcg
     7801 acgcctttgg tcatataaaa ctgggaggga ttgcccatta tatggcgaat attatctctg
     7861 agagaacggg ttatgatact agatccgttg ttctggggca ccttctgaga ggtggaaccc
     7921 ccacggcatt tgacagaatt ctgggcacaa gatacggagt tcacgcaatg gagcttgtca
     7981 tgaaaggcga ttttggcaga atggttgcac ttaaaggcaa tgatatcatc tctgttcccc
     8041 ttgaatatgg aatagctaca aagaagttag ttccctttga gtattacaaa ctgtcccaga
     8101 tcttcttcga ttgaggcgtt aagttgtatt attctgagcc ggtaaggatt gcaagatgcg
     8161 ctatggagga gtttattcgc catggtgttt taattggaac ccctgactgg gtttctgaag
     8221 agttcctgtc taggagagca ggttgtttcg tttctctcca tacatcaaac ggctcgctta
     8281 gaggttgcat aggaacgata taccctttgc aggaaaatgt tgctcttgaa ataatccaga
     8341 atgctatctc cgcatctaca aaagaccctc gattccctcc tgtagaggcg agcgaactag
     8401 ctgacataga tgtttccgtc gatattctta gccctccaga aagaaccaca ctttctggac
     8461 ttgatcctaa gaaatatggc gtcattgttt ctctgggtta cagaaaagga gtcctgcttc
     8521 ccgatcttga aggtgtagac acgattcaaa agcagctatc gatagcctta cggaaagccg
     8581 gcataaggga atcggaagat tacagtattt tcaggttttc agttgaaagg tatcgctgaa
     8641 accggtaaga ggtgaattca tgaaaccggc cgtttacttt gatgagtatg gcgaggaaaa
     8701 ggtactgcag tgtcttcttt gccccttgca ttgtataatc aagcccgatc aggttggctt
     8761 ttgtggcgta agaaagaatg ttgacggaat gctatattct cttaattacg gtcagctcac
     8821 ttcaattgct atagatccga ttgaaaagaa gcctctctat catcttttcc ctggcgagaa
     8881 gatactttca gttggttcat ggggctgtaa tttgagatgc aacttctgtc agaactggga
     8941 gatttcgaag caacgcccaa agacggtaat gcgtgttatg ccccagcagc ttattcaaat
     9001 tgctctggag agagaatcgc gaggtatagc tttcacttat aacgagccga ttgtctcttt
     9061 cgaattcata cttgatacct ctcgagcagc agccaaagaa ggtatatata gcatacttgt
     9121 tacaaacgga gtgattgacg aggaacccat ggatcttctt tcgcagtcgg taagaggtat
     9181 gaacatcgat cttaagggtt ggaatgacga gttttacgtg aaagacatcg gcacagaaaa
     9241 acgagttgtt cttcgatcaa tagagacagc caggaaagcg ggaacgcaca ttgagttgac
     9301 tacgctcatt attcccggca agaacgattc gcctgaagaa atgagggagg aagctaaatg
     9361 gatctcaggg ctgtctaagg atatcccact tcatattaac agatactttc ctaactacaa
     9421 gagcacaata cccccaactt cgcctgattc tctaatacaa cttgccgaag tagcaaagga
     9481 gtacttgcga tacgtttatg ttgggaatat cgatcttcca ggtctatcgg acacaacatg
     9541 tccgtattgc ggaaacctcc tggtcgagag aagaggtatg cgaagttctg tggttggcat
     9601 agatgaaaaa ggaaggtgca acaattgcca aaaagaaatc ccaatagtct tctgagaaac
     9661 cgaattttcc tgtatggact cctctttgtt tccgcaataa ttttgctgac aattgttttc
     9721 tttcgacaga agccattgga gtactatgac aaaaccgagt atcatctggg aacttatgtg
     9781 acaataagag tctcctcttc cacaatgtcc ccagttagac tcgcagaggc tgctatgacc
     9841 gagatcaaga ggatagatga caaatttggg agcagaaaca atggcgtaat cgctgagctg
     9901 aacagaaccg gagagcttca tgacatagat aaggaaacaa gttttctaat taggtcagca
     9961 ctggaaatag ctcggaacac gtccggtgca ttcgatccga ccctctattc tctaacaaga
    10021 ctgtggggat tcgatgatgc atcttctaaa aaaaggattc ccagcgataa ggagatcgaa
    10081 gatgctttgg gctcaacggg tttttcaaga gttacctacg atgaagagct gggatacgtt
    10141 tctctggttg atggagcgat gattgatctt ggagggatag caaaaggtta tgcagtcgat
    10201 atggcaattg caaagatcaa atcgcttgat ccggaagcaa caggtttcat cgatgccggg
    10261 ggtgacattg gggtaatcgg tcccaagtat gggaatagac catggatagt tggagtgaga
    10321 aatcctagag gagaaagtgt tcaagatgtt cttgaataca tctaccttta cgacggtgct
    10381 gtagctactt ctggagatta tgagcgattt tttattgaga acaatgtacg ttaccaccac
    10441 atttttgacc ccgaaacggg gttcccttcc cgtagcggag ttataagtgc aactgtaata
    10501 ggaaagagca ccatgctcgc agatgcctat gcaacggccg cttttgttat aggaatggaa
    10561 ccaggtgtta ctttcctgcc tagatttgga gcccttgtat tacttgtaat ggaagatatg
    10621 agtatcttta ggtctccagg ttttgaagtg tatcaggaga aatgaaattt accacgagaa
    10681 atgacgtctt gattgtaatc gtagcgtttg ctctggtgat cctttttgca tttccgcgtg
    10741 gaaatggctc ttctttcgca gaagtgtact gcaatggaag actggtaaga actatagatc
    10801 tgagctcaga ttctgagatt tttcttgatg taggaatggt tgttaaagtc cagggaggaa
    10861 agatttccgt gatcgattca gactgtccag agaagctttg tgttgcccaa ggagcagtaa
    10921 acatgcccaa tatcccaatt gtatgtgtac caaacagaac tttgataaag atagtctcgg
    10981 atcggtctga agttgacgcg ataactcaat agggacttag gaaaatcaag tgaaccacaa
    11041 acattcgtca agtgaaccac aaacagcagg cgagaaattt cgtgaagaat tgggatcaaa
    11101 aaagaaatcg agtcaatgat tttgtctcaa aactctggtt aaacgagtga ctgtggagaa
    11161 atgatggaag caaagagagt tactacacta tcgatgttaa tggcgatagg gtctgttctt
    11221 tatcttctgg aaagcctaat accgtttcca cttccaatcc cgggaggaag atggggcttt
    11281 tccaatcttg tcttaatcat agcgctggcc ggcttgcctt tcggcgacat cctgactctt
    11341 tcaataggca agagtatcat cggaagtttg ctggcaggca gactcgctac tccaggtttt
    11401 ctaatgggag ttgcaggcgc agtttcgtcc gcagcaacca tgtggcttat gaacaaaacc
    11461 ggaaagtttg gtctatttgg aataagtgta gcaggcgcag cgattagtaa tttctcacag
    11521 ctaatggtag cttcttcgct tattattaag aatatagaga tcatctttct ttacccctat
    11581 atgttggtac ttggatcgat ttccgcttca attaatgctt atgtagcatt tgaagtcata
    11641 agaaggatgg gtgattcact ttggcaagac taatactcgg ttcctcttct ccaagaagaa
    11701 gggagcttct gcatctactg agggtctctt ttgaagtcat acctccaaag ggagtagagg
    11761 agaatctttc agaaagattc tgcgaaaagg aattgtgcga actttctcac ttgaaggcca
    11821 aaaatgtcat gcttagaaat cctttttcca ctgtaattgc agcagacacg gtagtcgttc
    11881 ttgatggact tgttcttgga aaaccatctt cagtggaaga agcctatgaa atgctgcttg
    11941 tcctctcggg taggactcat tctgtcttca cctcagtttc cgtaatgtac gaagaaagcg
    12001 aattcagctt tgtggagaaa actgacgtaa catttagaga ggttccatcg gaagtcctca
    12061 aagaatatgc tgaaagcggt ctatcacttg acaaggctgg agcttatgga attcaggatt
    12121 atggtgctct tttcgtgaag agcatagcag gtgacttcta taatgtgatg ggacttccaa
    12181 taggaagatt gtggcatgag ctacacgcgc ggggggtaat ctgaattgga aggccagata
    12241 atgcctagag agaaactgct ggataagggc gctggcgcgc tgtcgaacag cgaattaatc
    12301 gcgattcttc ttagaacagg aatcaaagga aaagatgtac ttcagcttag tgaagaactt
    12361 cttggctact atggctctct aacggctctt ctttctgcag aagttaacga actactgaat
    12421 acaaaaggcc ttggaaaagc aaaggcagtt tgcttgaagg ccgctcttga gctgggacag
    12481 aggatcttca gagaaatgac tgagaaaacg aaagttcggc tggatgaacc tgaaggtgtc
    12541 taccatcttt gccatgatat ggcctttatg gatagagaaa ccgtaagagt aatttcccta
    12601 gacagcaaac tcaactacag aggattgaat acggttagtg tgggattact ggattcctca
    12661 cttcttcatc cgcgagaggt ctacaaacct gcaataagca ggatggccgc tgcaataatc
    12721 ctggttcata accacccttc cggtgatcca tctcctagca aagaagatga aggtgttact
    12781 tcaaagataa gggaagccgg agagacgcta ggaatcaaaa tgcttgatca cgttataata
    12841 ggaagcggta cattctatag ttttgcagca ggaagaacat ttaaagcgga ggtggcagaa
    12901 aaatgatttc aagagatcac gcaatgcagt taataatgaa caacatacaa tctaagaatc
    12961 ttattaagca cgtgctggcc accgaggcgg taatgagagc cctcgcagaa cgactcggcg
    13021 agaatgaaga tgtttgggca atggcaggtc ttctccacga tcttgattac gaattcacta
    13081 aagacaactt cgatcaacat ggtcttaaga ctgtttcaat gcttcaaaac gaagacttgc
    13141 ccgatgaggt caacgatgcg attcttgctc actgtgaaaa gaaagatcgg gtcaagctaa
    13201 tcgagaaagc aatatatgct gccgatccgg ttacaggatt catagtggcc gcagttctca
    13261 ttagaaaggg tagcaaactc tccgacattg atgtggattt tctaaaaaac aggttcaaag
    13321 aaaagtcatt tgcaagaggt gccagcagag agcaaatggc cagttgttcg gaatttggaa
    13381 tgtctctcga agatttcctt tctctttctc tgaaagcaat gcaagaaata tctgttgatt
    13441 taggtctgta gtctagagaa tattctggta tagagaggtg atgccatgct taaggcgata
    13501 atcgaaagag aagttagaag gtacaaagag gagtattctc ctatcaagag agaattaccg
    13561 gagaacctta atattgccaa attcattgat catacactct tgaaagcaga tgccactcca
    13621 aagatggtgg agaaattgtg tgaagaggca attaggtttg agttctggag tgtttgtgtg
    13681 aacactgggt atcttcctct ggttaacagg tctctcagcg gtagtacaat aagaaaaact
    13741 gtggttatag ggtttccgtt aggccagatg tcaagagaag tgaagggatt tgagacatcc
    13801 tgggcatgtg acaatggggc ggacgaattc gatatggtaa ttaatgttgg tttgctgaaa
    13861 tctggagagt acgaaagcgt atttgatgac atagctaaag tggtagaatc ggcttgtgga
    13921 aagactgtta aggtaattat tgaaacatcg ctcctcacag aagaagaaaa gatcgttgct
    13981 tgcgttattt cgagagaagc aggtgccgcc tttgtgaaga cctcgaccgg tttctccggc
    14041 ggaggggcaa cggtagatga tgtatcgtta atgaaattcg tcgtgggaga cgcttgcaaa
    14101 gttaaggctt ccggaggggt taaatcgaga gaggaagctc tcgcaatgat ttctgccgga
    14161 gcaagcagga ttggtactag ttcagggatc aatattgtgt cagaaggatc aaaagagaat
    14221 tgggggggtt attgagatgg atttctgcac attatcgtta aaggacttct tgaagaaagt
    14281 cgctgaaaaa tccgcaactc ctggtggggg agcagtcgga gcagttgtgg cagcgctcgc
    14341 agcctctctt ggctccatgg ttgcaaatct cactattgga aaaaagggtt atgaagacgt
    14401 agaaggtcac atggaatctt gtttagaggt attcgaatcg gaaagcaatt atctgtgtga
    14461 cttgatgaac cgagatattc aggcttttga tcaggtgtta tctgcgtata agttaccaaa
    14521 ggctaccgat gatgaaaaga atgcaagaga aatgaaaatt cagcaagcct taaagacagc
    14581 tatcgaggtc ccttttgacc ttgccaggag atgtaaaaac attatcgtaa atgttgaacg
    14641 tctcgcgaaa tggggcaact ccagtgttct ttcagatgcg gagagtgctg cccatcttct
    14701 tcttgcagtt catagaatcg ccaaggcaaa tgtcatgatt aatatgaaat ccctaaagga
    14761 ccctgattac ggtgcatgga taaacgaaga gatgaaacaa cttgaaaaac agattgattc
    14821 ctcatacaac aagataattg agataatcgg gaaacgtaat ggttgaactg aaactcaagg
    14881 caaagagtac ggaatcaaaa gctagaaccg gggtaattgt cacgcctcac ggtgaattcg
    14941 aaactccggt ttttatgcct gttggcacaa acggaacagt taagggatta tggcaagatc
    15001 agttggagga gcttgacgcg aggatcatac ttggaaacgc gtttcacctt ttcttacgtc
    15061 ccgggctgaa tgttctcagg aactttggtg ggcttcataa atttatgtcg tggaaacatt
    15121 cgattctgac tgacagcggt ggattccagg tcttctctct aaaagaaaaa aagattaccg
    15181 acgatggggt aaagtttcgg tcgcctcttg atggtagagc actcttcttt actcctgagc
    15241 tttcttcaga gattcaggag gcgattggat cggatattgc catggccttc gatgagtgtg
    15301 ttgaaccaag cgcaaacaaa gactatgtaa gacagtcagt aaagagaact acgatttggg
    15361 cggagagatt tctgctttct cacaaagggc aaagtatgca gtctctcttt ggtatagtcc
    15421 aagggggctt ttttagtgac ctgagggaag aaagtgccgc tagaattact tcacttgatt
    15481 ttgaaggttt cgctcttggc ggactgagcg ttggagagga tttccaaacg acggaaagaa
    15541 ttttgtcggg atctgtaaat ttgttacctg aagaaagacc aaggtatttg atggggatag
    15601 gttctccaga actcataatg gccgccgttg aaaacggcat agatatgttt gactgtgttc
    15661 ttccaaccag gttaggaagg catggagcgg ctttgacatc gaatggaaga atcaatctta
    15721 agtcctcaac aaacaaggaa gacaagagac ccgttgatcc cgattgcagt tgcaaggttt
    15781 gctcgacata cagccgagca tacatacacc atcttttcat aagggatgag attcttggaa
    15841 agatcctgtt aagctatcac aatattagtt ttcttatgca gtttgtgaaa aggatccgac
    15901 aagctatact tgacgacaga ctggccgaat tcaaagaaca ttgtattgag actggcctaa
    15961 ttcaaacaga gaaccttaag acttctgaag aacgtatggg gtgataagat gaagaagctc
    16021 ctgattctga tcgttgtgtt tttttgtgtt gttactgtgg caatgaatcc cttccagctt
    16081 caaacgggtg gaaatggtat ccttgcagta gagtatgatt cgggatttgg cagtccttct
    16141 gatttgtatg ggataactaa ttcagctttc tcaacgcact tcgttgaact gactacggtt
    16201 gcctctccct cttcaaatca acgtgctctc tacctgcatc tctttgagga ttcagatgat
    16261 ggaatggcag ggatgcttca atactacatg gacactgaaa atgcatcgaa tgttagatca
    16321 cttagctaca caatatcggg tgcgcttggc ccctttacgg cttacggggc ggattttgaa
    16381 ttaaacatga cgaccgccac ggcaacaacc tacggtgtct cagtcagggg aggtatcaca
    16441 ggtaaggcat atgaactgat tgaatacata gtttccttca gcagcgattt gtggaacagt
    16501 ggttcctccg taagttcagt cgatctgctc gctgggataa gatttgttcc tgaccctttt
    16561 atgattggac ttgaattagg cacaagagag gggatggcaa taaaatatct gggtctttcg
    16621 actcagtata catacaacaa tctcttttct gctcgtgctg gagtatctat gaacgctgat
    16681 cttttaagta acatagattt cattgttggc ggaggcatgg aagtaagggt tggagatatg
    16741 ctgataacag cagggattgg cactaacctt accaataaga ttgaatcttt tagcttcagc
    16801 aggaactgga gtgtcggcct gcttggccga tggtgataat gcctgcgtcg ccagacgtgt
    16861 cgcgtttgcc ttgagattcc tcaagagaac tattcggctg attttcctga tctagtcgat
    16921 ggttcatggt aaatatgctg aaccttttct gctggaacta ctttaggatt tatttggctc
    16981 ttttttggga ggccgtatgg atagaatagc tgttatagac tacggatcgc agtacacact
    17041 tcttcttgca agaaggattc gggagatggg cgtcttttgt acagttgcaa gtccagagga
    17101 attcaaagtc gaaagcgaaa ttaagggagt agttctttca gggggacctc agagtgtcta
    17161 cgaggaaggc gcgctcggtt ttcctaaaca actagaaggt tttccagttc caataatggg
    17221 catttgctac gggatgcatc tccttgcgaa aaagcttgga ggcgaagtcc gaagcggtgc
    17281 acttggcgag tacggactta caaatgtgtc tatggacaac gaacgttttc gtgacatacc
    17341 agaagaaata gtaacgtgga tgagtcatgg agacgaggtt acgtcgcttc cagctgaatg
    17401 taaggtaata gctagaagca agaacggcat aactgcaggt ttctccaacg gaagagacat
    17461 cgcttttcag tttcatccag aggttaatca cactcagttt ggaacaaatc taatcgaaga
    17521 tttcgtattt aatgtctgta atgcctctcg aaactggaaa cttagcgctt ttgctgagcg
    17581 caaagttgaa gagatcaaga ggatcgttgg cagctcgaaa gtagttggag gcctttctgg
    17641 cggcgtcgat tcaaccgttg cagcggtctt gacctctaag gcagtgggtg atgcgtttac
    17701 tgggattttt gtaaatcatg gacttatgag aaagaatgaa gatatagaag tctgtgaagc
    17761 tcttagaagc cttggtataa aactgataaa cgttgatgct tcggaagagt tttttcgaga
    17821 gttaaaggga attgacaatc ccgaagaaaa gagaaagatc attggaaaaa cattcataaa
    17881 agtcttcgaa agagaagcaa acaaactgaa tgcgagcttt cttcttcagg gtacaatcta
    17941 ttccgacgta atagaatcag gagccgcttc gaaaaactcc gcaaagatca agagccatca
    18001 caacgttggt ggccttcctg acaagatgaa tctgaagctt ctggaaccta taagagagct
    18061 tttcaaagac gaggtcagaa atttaggata ttctctagga attgaaccgt ctttactgac
    18121 caggcagcca ttccccggtc caggtctagg cgtgagaata ataggcgagg ttgattctga
    18181 gaaagcgaga atcctgaagg aagttgattc gatattcatg gatgttctca ggaaatcagg
    18241 tgaatcagaa aagatctggc aatcattcgc tgttctgctt cccgtcttca gtgttggtgt
    18301 taaaggcgac aagcggtcat acggttatgt tgtagcactg agagcggtga atagctcaga
    18361 aggtatgact gcgtcttggc acgaactgcc tcatgagatc ctcagagaag cctcttcgcg
    18421 aataacctca ggagtaaggg aagtcggaag ggttgtcttc gacataacag acaagccgcc
    18481 tgcaacaata gaatgggagt agaatatacg aagctcacta aagagaaaac agtggctgct
    18541 cactctcaaa atgaaactgc tctcaaaaaa cgaaagacaa tcgttataga aactctgggc
    18601 aagagtcaaa tgtgttctct tgcttcctgt agaggcaaca agtgcgctca aaaaataaag
    18661 acgccgaacg gcgtctttct ggagcggaag acggggttcg aacccgcgac cccctgcttg
    18721 gcaagcaggt gctctaccac tgagctacat ccgcaaatga aaactagtct ctctggtgcg
    18781 agaggaggga cttgaaccct cacaggtctc cccactggat cctaagtcca gcgcgtctgc
    18841 caatttcgcc actctcgctg gtgactcgcg cgggattcga acccgcgacc ccctgattaa
    18901 aagtcaggtg ctctacctgc tgagctagcg agtcgctccc actttcagtc tccagcctgc
    18961 gctggcagaa agattatatc atcacatcat ctgcctatca agtggagaga attattaaga
    19021 aactgtgaaa gaacaagcgc ttcttgaagc ctccacaagt agcaagagtt gaagactgat
    19081 gatactatct acttaggagg tcataacatg gttttacttg ctgacattgg taatacaaca
    19141 acggttctag gacttaccgg cgagaagagc attgaggaag tctggagact cccctcatca
    19201 agaatagaga ctgaagatga actctttgtt atccttgatg ggcttcttag gattaagggt
    19261 tactctgtcg gagaaataga tggcctgtgt gttgcaagtg ttgttcctag gttgaacaga
    19321 acaattaact acttcacaag aaagtatctt ccgaaacctg cagtcttctt gaggccggat
    19381 agatttttcc ctttagggct gaatacggat aacccagccg agattggggc tgaccgactt
    19441 gccgatgtct taggggcaaa ggaatgctat ggaaacaatg tgatagttgt cgatgcgggg
    19501 acagcaatta ccatagacat cgtaaaagac ggtaattttg tgggaggagc aatccttccg
    19561 gggcctggga cagcaatgtc cgcacttttc tcgcataccg caaaactacc ggaagtggag
    19621 ctcttcttcg aagaccacta ctttggaact aatacagaag acaacttgag aatcggtatt
    19681 gtaaacggaa catacttcgc aattcaaggg attattgaca atattatcga gaaattcgaa
    19741 gaaaagccaa cagttgtggg tacaggcggg aatatcgatc tcttcttaat tgagggaggt
    19801 ttgatagaaa tcgctgatcc gaatctcaca ctcaaaggct taagtttcta ctataacaga
    19861 gtgaaaccat ttgaagaaga tactacttgt taacccgtgg atagaagatg tgtcggccta
    19921 tgactattgg ctaaaaccat taggtctgct gtacatttct tcactcctga agcatatcgg
    19981 gatccaatca attctaatag attgtctcga tagatatgat gatgaattga tcaccttttc
    20041 tccgaaattc ggagagaaat attacggaac ggggaaattc cttgaaagcg agataccaaa
    20101 accccagtct attgcctcga tacccaggag attcaagagg ttcggatttc ccgaggaagt
    20161 attgagaaag aaaataagac aagtaaagaa cgttgattgt gtctttgtca cttcaatgat
    20221 gacttactgg cattatggag tacacgatac tattaggatt attaaggacg aaatgccctc
    20281 tgttccagtg atacttggag gagtttatgc aacactcctt cccgaacacg cccggcggta
    20341 ttccggagca gacaaggttt gccccggaac cggaacggaa ccactcaaaa tggctcttgg
    20401 tttcctggga atcaatgaaa cactcgattt tgactggttc gacgacctcg atccagacta
    20461 ttcggtttat aaatctgcaa gatatgccgt gttgatttcg agccttggct gtccgttcaa
    20521 atgcacatat tgtgccagca gcctcctctg gaacagcttc gtgagaagag acccggtaaa
    20581 gagtgccagg ttcgtcaagc atcttactgc atcaaagggg ctctcagata tcgtcttctt
    20641 tgacgatgca attcttgtag gtagtggttt caagagactg atggaggagt tcgagcgtct
    20701 tcgaatcaag gcgagatttc atctgccaaa cggcgttcac gccagattta tcgatagaga
    20761 aaccgccgat ctcatgtaca tgaacaattt caagacgata agaatcggtt tagagacttc
    20821 cgatccggtt atgcaaaatt ccacaggcgg aaaagtcaat ctagacgata tattcgtagc
    20881 gattgagaat ctttcatcag cgggattcac ttctcgagag atcagcgcct atattatggt
    20941 taaccttcca ggccagagcg aagaagacgt tctggcttct cttagaatct gcgaagaact
    21001 aagaattagc cccagtctca acgaattcac acccattcct ggaacggtgc agtggaaaga
    21061 actaacccgt ggcggcgtat tcgacgagga tatcgaccca ctccttttgg acaactcgat
    21121 ccttccatac tggtggaagg agggcttttc tcctgacgct gttcagaggc tgaaagagaa
    21181 agcgtggtca ttaaggagaa gattaaatga ctaaggatct attcttaggc tttgggagta
    21241 acatgggaga aagactgaat ctcattatta aagcctttac aatgctaata gaaagggaaa
    21301 tcacgctgca atcagtatca tcgctatact tgacagaacc attaggcaat atcgagcaaa
    21361 atgaattctt gaactgtgtg gggaaattcg aattcaaagg tgatccacac ttcttgcttg
    21421 aagagatcaa ggtaatcgag aagtctattg gaagagttga aaggttcagg tggggtccgc
    21481 gagaaatcga catcgacatt ctgctctttg gagaaatgat tctgacaact gaaaagcttt
    21541 ctatacccca caaagatata ataagaagga aattcgtttt ggtcccttta ctcgaaatcg
    21601 acgaagagct cagagaccca agagacaaca gtctcttttc ggatcatctt gaaagccttg
    21661 gtaaagaggg atggcctgag ctcttcctag aagctcgtct ttttgaaaaa ctaatagaat
    21721 tagaggtgaa gagatgaaaa tacgaaactt cagcataatt gtaatagtgg ttctaattac
    21781 gaccttgttt tcctcctgta gtgcctcgaa atcagaactc gtacccggaa agtttgcggg
    21841 gttcgcgcta agcgactata tggatatctc tacttcggtt agtgttggcg gcatgatctt
    21901 agggatagaa ctaaatgaag atactctgct tctccataaa gagttcgcta aaggcacaaa
    21961 ggaattcaat gtttacgtag caaaatttga gaatgcttca agagccacag gtttctggta
    22021 tgactttgtt agggaaattt caaatgaaat cagatcagtc ataagcgcga ttccatggat
    22081 ctacggagaa ttctccggtg aagtcggcga gagcttcatg aagtcgtggt tttccggtag
    22141 atggctttac atttttcttg gggacaatag tgaggaagta gactctgcgg taagagcctt
    22201 caaagagtat gagaagagtc tggtgagatc agttgagtct taaaccatac aatgacctca
    22261 agactcattt gaaatcacga tatggagaac ccgttcatag agttcccctt gatgcaggct
    22321 tctcatgtcc ccacaaagta aatggaaaag gtggatgcat atactgtgat tcctcgggaa
    22381 gtggtttcac agtagactcg actctctcgt taagagagca gctcgaaagt aggatggaat
    22441 tccttcgaaa aaagggtatt aggaaattca tggcatattt ccaggccaac tcaaatacct
    22501 tcgcacccgt aaagaaacta cgacagcttt acagagaggt tattgttgat gacgttgtca
    22561 tcctcgacat ctctaccaga cccgatcttg tctctgatga ggttctagat ctacttgaag
    22621 aattcacttc tgacgttgat gtgattcttg aactcggtct tcagagtatc aatcccaaca
    22681 ctatcaaaac cattaacaga ggtcacactc tgtcgcattt tatcgattcg gtactgagaa
    22741 tcaagaaaag aagaataaat ttggttgtac atatgatatc gaatcttccc tgggatactg
    22801 aagaggatgt tgcggaggcc gcaagactga tctctgtact cggtatagat ggcgtcaaac
    22861 tccattctct atacgtagtc aaaggcactg aacttgcaag aatgtatgaa gcaggtgaag
    22921 ttaagatagg aagtgtagat gaatttctgg aaagagtagt ggtatttctg gagcatctag
    22981 actggagagt cattgtacat agacttgtct cagaccctcc taacgaagga acaatgtttg
    23041 gaaattgggg aatgtcaaaa atcaagctat tgaatatgtt agaaagaaag atgataattg
    23101 aaggtagata ccagggaagc aaggctctat tttatagatc gggaagcgaa aagagtgacc
    23161 gcggaaaatc cgaggaaagt ccggactcca tgggcagggg cgctgggtaa cacccaggga
    23221 gagtgatctc cggaaagggc catagaaaat tagaccgcct tcgggcaagg atggaaaggt
    23281 gaggtaagag ctcaccggct gtcgggtgac cggcaggcta ggtaaccccc gtctggagca
    23341 agtccaagca gaaagggtct gaggatgccc accgattccc tttcgggtag gatgcttgag
    23401 ggttatggta acataactcc gagatagatg gtcacgagaa caggatccgg cttacgtttt
    23461 cgcttcccgc gtttttttgg agtgtctaat gaatattccg atgactagga gcgatatggt
    23521 cgagagggga tgggaatgtc tcgacatcat tctaataacg ggcgatgcat ttgttgacca
    23581 tccttctttt ggaacggcga tgattgcgag agtgctggaa tcacaaggct ggaaagtagg
    23641 tgtcatttcg cagcccgact ggcgaacaaa cgaagacatc tcagtgttgg gtaaacctgc
    23701 tctgttcttc ggtgtcacct ctggtaacgt tgattcaatg gttgcaaatt atacatcgac
    23761 tggaaagaga agaaggagcg acgattacac tcctggaggt tttggtggaa agaggccgga
    23821 tcgggcaacg attgtttact caaatttgat tcggcaggtc ttcaaagaaa ctcgtatagt
    23881 tctcggtgga atagaggcca gtcttagaag gttttctcat tttgactggt ggagcaacag
    23941 aataagaaaa tcgatcttgc ttgattccaa agcagacttg ctggtatatg gaatgggtga
    24001 aaagccaatt ctggaaatcg caaccaagat ctcgaaagta agagaagtcc ccacagatgt
    24061 tgaaggaacg gttttttggt ccagctctaa acccgcagga attgaattgc catctcatga
    24121 agaagtatct ctagacaaga agaaatactt tgaaatgaca aaagtaatgc atgaagagac
    24181 agacccggta aggggacacc agttgttcca gcttcaagac aatcgatatg tagtccagaa
    24241 caagccaccc aaagttacgt cagaagaatt agatgcttac tattccttgc cgtttaccag
    24301 aagagttcat ccagtttcgc tttcaagggg aagggtgaaa gcattagaga ctgtacgtta
    24361 ttctgtgaca agtcataggg gttgttatgg acaatgcaac ttctgtgcga tcgcttttca
    24421 tcaaggtaga accgttgttt ccaggagtga agattcgata attcaagaaa tatcttcatt
    24481 caccagagac gtcggattca ggggaactgt tactgacgtg ggtggaccta ctgcgaacat
    24541 gtacggctac gaatgttcta taaaggaaaa agtcggtgca tgtgcaaaaa agagatgcct
    24601 ctttccagag gtgtgtgatt ctctaaaacc agatcattca agatacctta atctacttag
    24661 aaaaataggg aaaatccccg gtgtgaaaca tgttttcgta tcatctggaa tcagatatga
    24721 cctcattcta aaagataaaa gatccggaag ggattttctc aaacaacttt tgaaaaacca
    24781 cgtttctggt cagttgaaaa ttgcgcccga gcacacagtg cagaaagtcc ttgaatacat
    24841 gggcaaaccc tctaaagatg ttctcttaga ttttctcaaa gaggttaggc tcctttctaa
    24901 cgggaagtgc cacattatag gctacttcat agcggctcac ccgggatgca caaaggaaga
    24961 catgattgaa ctgaagaggt ttgtcaagag agaaatgcac tataaccccg agcaggtgca
    25021 gttgttcaca ccgacaccgg gaaccatctc cacagcaatg tacttcaccg aaatggcttc
    25081 agagaagggt gatccagtct taactgaacg gtcgacaggt ggaagaaaca gacagaagga
    25141 aattctggta cgcaaagaga tcctagatag aaaacctgaa aaggatataa agggtaccgg
    25201 ccacaagcgt aataagagtg acaggcattg agaacctaag aaaatcccca tatgtgagtc
    25261 tttcctttga atgcttttct attagaccca ctccaaccat gttagctgcg gcaccggcta
    25321 ttgaaaaact gccacccaga caggccgaaa tggagatcac ccaccaaata tctgccggaa
    25381 cgccgaactt agttgtcatc ccctgaatca ctggtatcat gaaagttacc gcaggaaccg
    25441 ctcctataaa gccgctcatt attgccgaca accagtaaag cgtcaagaac aacagtattt
    25501 tgttgccgga gagagagcca agcaagtcgg aaataacaga ggtgatgccg acctcctgga
    25561 gtgcaaatgc aagaacaaac aggcccatga agaaaaatat cgtatcccat tctatatctt
    25621 cggaaagatg cgaaaagctc tttccgttaa gaatcatagc cgctgccgca ccagcaagag
    25681 caataagaga aggttccact ccgattagac catggagaag aaagcccaca attgtagcga
    25741 agaacacagc taatgacttg tagaaggctg acattgaaac tatcgctttc ttaggatcca
    25801 tgttggctag acgctgcagc ttttctccca tttctctgta atctttgaaa acatgtcgat
    25861 caaggtagat tagcgcaatt atcaacgcaa atagtgtaat cggcccatca attcttatga
    25921 agtccatgaa tccgaaccca cttgctgagc cgataagtat gtttggtgga tcaccaatca
    25981 aagttgccgt cccaccgata tttgccgcta gaactccagc aaggatgaaa gtgcgaggag
    26041 aaacctccaa agcatctgct acaagaaaga ttatcggtgc aaaaagaaga atcgttgtga
    26101 cgttatctag aaaagcagaa aagagtgcaa ttgtaaccac aaagaatgta agcaaagctc
    26161 tcacattacc cttggagaac cttactacgt gaacagcaac aaattcgaaa aaaccagtcg
    26221 tcctcaaagt gccaacaact atcatcattc caattagaat actgatagta ttgaaatcaa
    26281 caaggtagcc gagctccttc atatcgaagt caggtataac tttagacaaa agtattagaa
    26341 taccagcagc aaatgcaact gttgatctat taaacttacc agaaattatc agataataag
    26401 cgcttatgaa aaccactaga acaaacaccc actgcaatta actcacctct ggaagacata
    26461 actttatcag tatatcacct tctcattttt tgaacaacga attcctctgc tcagtcaaat
    26521 tcttaccgga gtagccaaaa taatgaagga ggtcagagtt tatgagaaaa gttcttgcag
    26581 ttcttattgt tgtaattctg tcagtggctt ctctggccct ggttaacgaa ggctacgaga
    26641 gcccaataac caacgtagtc caagcagcag gaccagcagt tgtcaaagtc gatgtagagg
    26701 ccactaggaa atattcaatc accgatcctt acggtttcga ggatttcttc aggcgatttt
    26761 tcggagagat ccccggccag aaggtaaccg gggttggctc gggtttcatc ttcaacaagg
    26821 atgggtatat tttcaccaat gagcacgtag tttcagacgc ggaaaaaatc acggtttctc
    26881 tacttgatgg aagcacctac ccggccaaat acattggcgg agatgaggag ttggacattg
    26941 cagtgataaa gatcgatccc gatggagttg atctccccac tgttgaactt ggtgactcag
    27001 acgcgctgag aatcggcgaa tgggcaattg ctatagggaa tcctcttggc cttaagcaca
    27061 cagttactct cggagtgata agtgcggtag gaaggcagct gccaaaacca gatggcaacg
    27121 gagtatattc taatctaatt cagacagatg ccgcgattaa tcccggtaac agcggggggc
    27181 ctttgctaaa tattcatggc caagttgtcg gaataaatac tgccataatc gctcctgaag
    27241 ttggaaccac ccttggattc gcaattccca taaacgtagc tctcagattt gtcgattcaa
    27301 ttatcgagac cggatctgtg caaagagcat atcttggggt ctatatggat acggtcactg
    27361 aggaggtttc aaaatccctc ggactaaagg ttgacagcgg agcattaatt acagatgtgg
    27421 ttcctggttc agcagcagag aaggccggaa taaaaccaca ggacgtcatt attggctttg
    27481 agagtctaga gatcacaaac tcagcggagc ttagggcagc agtattgaac taccctgcag
    27541 gctccgaagt gaagattaca atagacagat tcggagagag aatcgtactg actgctgttc
    27601 taggatcact tgctcaagaa agcgagtctt cagcaacaga agacaacgga gcaagacagg
    27661 cttttgtgag ctcactcggt atatcggttt ctgaaataac tcccggcgat agggaacgtc
    27721 taggaatacc tcaggaattt agaggcgtaa tagtaagaga agtaaatcca gaaggaataa
    27781 tctacaggtt gggaataggc ccggacgata taattactcg tctgagcata aatggcaatc
    27841 aagaatcgat cgagagcgtc aacgatttcg agaagtatac tgaaaagatc aagaaagggg
    27901 actacgtagc gttctttgtt tacagaaacg gtgtaagatt cgtggcttct ttccagctct
    27961 aaaacgaaaa tgccgccacg ctttgactag tggcggctta cccccttaat cccctgtgca
    28021 ggattaacgc cttgattata gcacaggggt tttttctttc caaaaccatc tcttgagtag
    28081 atttaattat gatgcattac gcccacaata cattatattg atgaaacaag cagaatagct
    28141 ttcgcagcca gttttccttc aacatatgca gatgtttcca gcttagcgac gttcatccta
    28201 ctgtcgatca atttgacaga gtactcaatt ctgcacggcg gacgtactat ggtcttgaaa
    28261 cgagctcttt ctattcccgc aaacaacggt gtttcatttg gtttcagcaa aagaattcct
    28321 gcagtctgag caagaccttc aagaatcagc acacctggat aaatcggata accaggaaaa
    28381 tgaccctcaa aaacaggatc gttttctagc aagtctctga aagcaagaat ttcggaatcg
    28441 gtcattgaaa tcacgccatc gacaagaaga aagggctccc tgtggggtat tctttccttt
    28501 acaaactcct tgtctctcac aaatcacctc cgtttgattt tcgaactgtt cgctcgccag
    28561 cagtcctaga ttcagacaaa aatacggcaa aaggtcctgg tatgttgttg cttttgttaa
    28621 accagcatac ttgtgccagg aggatctact ttgaatgagc aaagacagtc gctaaccgaa
    28681 atgcttggaa tcaaataccc cattcttcaa ggagggatgg catgggtctc ggaacataat
    28741 ttggctgcag cagtttccaa tgccggttgc ctaggcataa ttgccggagg gaatctttct
    28801 ccagaggatc tacttcatga gattcgcgag atcagaaaat tgacttcaaa gcccttcgga
    28861 gtcaacgtaa tgctgctttc tccatatgcg caggaacaga tagagataat ctgtagggaa
    28921 agagttcccg ttgtaactac tggggcaggc agtccatcca gctttattga aaaattgaag
    28981 acggccggaa ctaaagtgat tcccgtggtc gcctcttccg ggctcgctag aaggctagag
    29041 agacagggag tcgatgcagt gattgccgaa ggaatggagg cgggcggcca catcggaaag
    29101 gtttcaacaa tggtattggt tccagcagtt gtttcagcaa cgagaatccc agttattgca
    29161 gcaggtggaa ttgctgacgg aagaggatta gcagcatctc ttgcacttgg agctagtggt
    29221 gttcagatgg gaacaagatt catctgcacg actgaatgta gagcacattc tagatacaag
    29281 gaacggattc tgttatcaaa tgaactagac accgtcgtta cgggcaattc aaatggtcat
    29341 cctgtaagag gatttaggaa caggctaaca agacgcattg aagaactaga aaagcaaggc
    29401 tctggattcg aggaaattga aaaaatagct gtcggagctc tcaagagagc tgtcgaaaat
    29461 ggtgacataa gtgatggttc catgatggcc ggtcaatcct gcgggcttat tggagaaatc
    29521 cttgatgttt cacaattagt tcagtcaata attgatgagg cagaagaaac aattcgattc
    29581 ctgggaggca tagagaaatg gtagcatgga tttttcccgg acaggggagc caatacgttg
    29641 gcatgggtaa gagtttttgc gatgaaagtc ctttgtttag agagtttctt gaactatccg
    29701 gacagttgct gggttttgac atgttaacaa tgatgctcga ggggcaggaa gacgaactaa
    29761 agctcacgca taatgcccaa ccggctattc tttcgctaag tatagccctt tccgatgctc
    29821 ttattgagag gtggggaaaa cccgacattt gcgccggatt atcgctgggt gagttctctg
    29881 ctctagtcgt agcgggcaca cttagatatg ccgacgcact tcgtcttgta aggttgaggg
    29941 ggacagctat gcaacaattg atgtcttctg aagatggaac gatggcagcg ataattggtc
    30001 ttccagaaaa agtagtcgaa gagatctgcc ttgagatttc ttcaagtgaa gaggttattg
    30061 tagccaatta caactgccca ggtcaagttg taatttcagg gttatcagaa aaagttcgaa
    30121 gtgctatgaa caaggcaatg gaaaagggag cgaaaaaatg tgtggagctt tcagtgagtg
    30181 ctcctttcca tagccgatac ttgcgtccag ttggagaagt tttgagagag ttcttgaacg
    30241 gcattgatgt caagcctccg gaaatccctg ttatctcaaa tgttaccgga gagctttttc
    30301 ctaaagatcc aaacgcgata agagagttgc taattgaaca ggcttacagc cctgtcaaat
    30361 gggaacaatc tattagaaag atgatcgaac taggcgcaga tcggttcgtt gaagtggggc
    30421 ctggaaaagt gcttagtggg tttatgaaga agatagatag gagtgtctct gtagaaagca
    30481 cagaaaacga aggccttgga atacttaaag aactacggga aatcaagaac tgaaaactaa
    30541 actttgagag gtgagctcag tggaaaaaac atcaacaatt caggaaaaag tgatctcagt
    30601 cgttgcagat agtctgggag tggaagctgg tgaaatcagt ggtaattcga gatttattga
    30661 agacctcggt gcagattcac tagacttggt agatcttgtc atggagcttg aagacaaatt
    30721 cgaagttcag atcagcgaca gccaggcgtc tagcttcagg accattgatg atgttgtcaa
    30781 attcataagc acgctcgatg aaaaataaga tgaaaagagt cgtagttact ggaatgggag
    30841 ttattagctc gatcggaact aatcttacag aattctggga atcgcttaaa tctggtacct
    30901 cgggaataga ctggataagc acatttgacg tgtccgaata tggggcaaag gttgccggtc
    30961 aggttaagaa tttcgatcca actaaataca tggatagaaa agatgcaaag agaaatgcaa
    31021 ggtttgtcca gatggcaatc gcgagcgcaa gtgaagcgta cgatgattcc gggctgggga
    31081 atagccgaat aaactccgaa agagcggggg taatttacgg cgtgggactt ggtggtatgg
    31141 atgtgataga agatcagcat tctgttctaa tgaacaaagg tccgtcaaga atcagtccct
    31201 tccttatccc tatgacgata gcaaacatgg cccccggact tttggcaatt cgcttcaatt
    31261 tcagaggcac aaacttcacc ctttccacag cgtgtgcctc ggcaaccaat gcaataggtg
    31321 aagccacaag gcttattaga ggcggaattc tcgatattgc catgacgggg ggttcggaag
    31381 ccacagtaac acctcttgct ctagctggat tttcgaatat gaacgctctt tcacgtggag
    31441 ctcctaaaac ggcttccaga ccgttcgatc gtgatcgtga tggatttgtt atggctgaag
    31501 gatcggcaac tttgatcata gaagagtatg aacatgccat gaaacgaggc gctatgatct
    31561 acggagaggt tgttggttac ggctcaaccg acgacgcatt tcacattact tcaccggaag
    31621 agtccggaga cggagcgtac agagcgatga aaatggcttt agaagaagcc ggagttgatc
    31681 cctcagaagt tgactacatc aatgctcatg gtacttcgac tccactgaac gacctaatgg
    31741 aaacccgagc gattaagcgt ctatttggaa atcgacaaga cttgaacatt agttcgacga
    31801 agtcgatggc cggtcacgca ctaggtgcag caggagccat agaagctgtg gcaactctac
    31861 tctcaatgaa aaattccttt attccaccca ctataaacta ttcgagtcct gacccagagt
    31921 gtgatctgaa ctatactccg aactttggag ttaaaaaaga attacatttc gtacagaaga
    31981 attcctttgg atttggaggt cacaacgctt cccttctctt caagaagcta taggttgcct
    32041 gttccgggtg gggtgctgaa gcgccccact tcttcatagc ttggaaactg tttctttgaa
    32101 gaatgagctt tcgttttcta tttgaccaat aacaatatcg gcaataactg agagatctaa
    32161 tgattttcgt ctccaccggg atctttcttc gcgattttct ctgacaatac tctcagagca
    32221 aacttcacag ccttagtcct gaacggtctt gcttggctat ccagtttgat gagccaacga
    32281 ttccctcgcc ttgatagatt tatgtagaat cgctgggaga agtccctatc gactgttagc
    32341 gtttcaatca agaaggtatt gcggtccata tttctgtaca gccccatgaa tttcacaaaa
    32401 atctcatttt tcgaaaaact cttcccgtcg actttctgaa gagatttctc gtcaatgtca
    32461 tcgatccaca agttcgacac acgaaccacg tcctcttcac catttatttc gcatttgatt
    32521 cttatgcatt tcatcctaaa gatctcttta ctcatatcca gccatttctt ctcgtatctt
    32581 gttcctactc cgaccgtagg attcacttca aggactggat catcgaaaca gttcgtcttt
    32641 ccaacttctc caaacatttc atgaacaaac tcttttgaat ccgaatacag ctggaaaaaa
    32701 ccaccctttt ttaacacttt ggcaatcttc tcaaggaaat taactgaatt gagcctccgt
    32761 tctgaatgtc tcttttttgg ccacggacaa ggaaaattca tgtatactcc cgacattgaa
    32821 tctttaggaa gtatgtttcg cagagcaaaa gatgcatcta caagcagtgc gcgaacattt
    32881 ccaacatgat tccttgagag tgacttcaaa agctttttgg cacttatcat tgatagctct
    32941 acaccaagat aatcggcatt ggggttctca attgccttct gaagtatgaa ttctccgctc
    33001 ccaaaaccaa tttctaagaa agtctctctc tttcttccaa agacatcctg gagatcaaga
    33061 ggaagtttat attccgtaca atccacatag ttacggagat agtattcact catgctttcg
    33121 ccccgcccac aaatcagaca tttatttcta ccaaatttcc ttgcttgaca aaaagcattc
    33181 taacgcaatt gtgcatcaat ttctacattc agctttttgc tattggatcg catcatcatc
    33241 ctccgatggt ataattatcg tgactcccgg aggtgaagga aatgctcttt gatcctgcaa
    33301 gaatcgaagt cctttattca gaagaggaac tgaagaaaat tgtcaatgga ctggcagaga
    33361 agattaatga atactattca ccgattactg atgaaattac tgcagtatgt atcttgaagg
    33421 ggtcggttca tttttacagt gacttactta agcggctgaa tttgaaggtc cgatacaact
    33481 ttgttcatgt ttccagttat tctggagcat caactaccgg taggattcgc gttaaaacct
    33541 gggtagatga atcggttacc aatcgctacg tcttgcttgt tgaagacata gttgacacag
    33601 gaggaactct caggtacatt atcaactata tctggaagca aaaaccagcg gatgtaaaac
    33661 tagtatcgct tttcgaaaag accattcacg atcatggagt caaaattgat tttacaggtg
    33721 aaaagattgg ggatcagttc atagtcggtt atggacttga ctacgaggag gtttgtcgca
    33781 atcttcctta cgtaggttat ttgaaaggag aataatgaaa aaaggcaatg ttgttacact
    33841 agttctggct gtactgcttc taagcatttg caccataact tccctttttg cattgaacgt
    33901 tgtcagttcc aacaggaaaa acacccagct gatgctagaa gcttcaataa tgagaggagt
    33961 aagagtctca gcagaaaagc ttcttctgtt cagtatggaa catggaaaaa agctggctgt
    34021 tgagatcaac ggttatcact tggaaacgga tgagattaat gggagttggt gtgtgcgatt
    34081 agataatgga gataaagaag aaataatatt cgccgaaggc aggtgagcga tttgatagca
    34141 atatccgttg attccggttg tgcacctagc agggattttg ttcgggagca taagctattt
    34201 ttcatgggga tgaaggtgat aattgacgat aaggaataca ccgatgactt tgacttcatg
    34261 gagaatgtgt tttatagaat cgttgaaaac tcaaaggaat ttcatacggc gcagccaccg
    34321 ttgggtcagg tacttgaata ttataacgat attaggtcaa gaggatatac agagcttctt
    34381 gatattcact tttcatcaaa gatgtcaggg gtgtacgaaa cttgcgtaat ggcttccaaa
    34441 atggtcgaag gaatagatgt tcgggtggtc gataccagaa aagtgtctat tggtgctaat
    34501 cttgtggcca gaaagcttat caagctcgtt gaaactggat gtagtctaga cgaggttttt
    34561 gaaagcgtac cccgtgtcat tgataatact tttatggagt ttagtgttcc cactttgaag
    34621 tatctcatta agaatggaag aataggaaag gcacaggggc tcgcaggaac cttgctaaac
    34681 ataaaaccaa ttctttctgt tgatgaagaa ggatttataa gcccaatagc aaaactcaga
    34741 ggtatgaaga agcttcagga acagatggtt cagaacatcg tggaatttct tcaaaacagg
    34801 agaaaatcaa tcacgcttta ctcgatttat ggaagcgaag agtatttgac cgttatgcat
    34861 gagacaactg aatggcttat agaatcgatc gagtcgagac ttggaatatc tagaagtgac
    34921 attgagctgg tcagcggtag aatatggcct acgatagctt gtcacagcgg tcctgcggtt
    34981 tttggacttg cttgctacgg ggagagagaa gcagaataat gctcttggag caattccttg
    35041 aagaatgtga aagacttctc gaagctcacc tcagcggtaa aagggaagat aatgtattac
    35101 ccgagctcaa gcgtttccta tctttgctcg ataggtccga gttgtctcat ttctcctcgc
    35161 ttggcgaata cttgtctaag tttatttctt acctctccag aattgacgag tttcctcatg
    35221 atcgaaaggt caaaagattg aaaaacggcc tcgaaatgat agccaagcta aggaattttt
    35281 ttctacttca caattcagaa gaaattgaat ctcttcttgg tagaccggtt tcaccttcga
    35341 cacccattaa gtacgcatat tcggtaggtg aagcgagatc aaagatactg aagagaatgg
    35401 atatcgaaac aattggcgac ctaatacaat atttcccgag agattacgaa gatcgaagga
    35461 taattatgcc catttcctcg atagttcctg accgaaaggt aagtgtaaaa ggccgtcttc
    35521 tgaacttctc tgcaaagaag gcatcgggtt atacaattat ctcagcagta gtcagcgatg
    35581 gttttggtca gttgcttctt aagtggttta atcaggacta tattattcag aagcttagga
    35641 gggaccgaga gtacttgatt cacggtttgg caaaggaaac tccatttggc cccatggaga
    35701 tgaactctcc agaaatagag gagatccaag gcgaagtgcc aagagaaatc cttcctgttt
    35761 attcgctaac ttcgggcatt tctatgaaga tgatgagaaa gatcgtcaaa cgaaatctcg
    35821 gcttggtaag atcgttggac gatctagtac cttcctcaat aaccactgaa aggggattac
    35881 ttcccagaaa acatgcgttt accgctattc actttccaaa gagcctttat gaaattagga
    35941 aggctagaga atcgctggct tatgaggagt tctttctgtt tgagactaca atcttattca
    36001 gaaaacgcca gattaggaaa gagtatcagg gtctccaaaa ggaaatcagc ggcgtcttgt
    36061 cgaagagact gatcgaatcc ttgccatttg aactaactaa agatcaggtt actgctttcg
    36121 aagaaatcag agatgacatg agggccgctt cgcctatgaa tagactactt caaggtgatg
    36181 ttggctccgg gaagactctg gtcgctgaac tggcaatggt cgataattac gaagctggct
    36241 atcagagcgc cttgatggta cctacttctg tacttgcaat gcagcactat gagaagatca
    36301 agagagaact ttcgcctatt gggattgaaa ccggtctgtt gacgggatca ttgaaaaaaa
    36361 acgaacagga ttttgtgagg atgaggttga agaagggaga aatagatgtg gttgtcggta
    36421 ctcacgcact catccaggat ggtgttgaat ttaaaaatct tggccttgtg gttgtcgatg
    36481 aacaacatag atttggggtc aagcaacgag aaacattgac aacaaaaggc aagcttctcg
    36541 acagtctcgt catgaccgcc actccaattc ccaggactct tgctttaacg gtttatggag
    36601 acctcgatat ttcgacaatt cttacattgc caaaaggaag atccccagtc agaacaatta
    36661 tactcgcaag aaaaagactt aaagatcttt actcatacat ctcagatgaa ctgaagatgg
    36721 gtcatcaggc atttttcata tacccgctca tcgaagaatc agaacaggtc gatctgaaaa
    36781 atgctaccga tgaagcaacg aaacttagag aagaagtatt cccaggggta ggtgttgaat
    36841 tgcttcacgg caggttaaac gataacgaga aacaagagat aatgcagaga ttcagatcaa
    36901 ggcaaagcat gattctcgtt tcaacaaccg ttgtcgaagt tgggatagat atacccaccg
    36961 ccactgttat ggttattgaa caccccgaaa gattcggaat ggctcagcta catcaactta
    37021 gagggcgtgt cggtagaagc aatcttaaat ctatctgtgt aatggttatg aataaagcta
    37081 tttcagatga tgctctctca aggctcagag aattcgcttc tacttcgagc ggattcgacg
    37141 tcgccgagct tgatctaaga cttcgaggac cgggcgagtt tcttggctta aggcagcatg
    37201 gtatgcctca gttccttatt ggtgacattg taaacgatcg agatctcctc ttcaaggcaa
    37261 gagaagacgc aaaacaactg atggaagaag accccgaact ccttgagcac ggtgctctac
    37321 gaatcgaaat ggaaagggtc tattcagaga gagctagact gattgaggta ggatagcatg
    37381 ttgacaataa ccggcggaaa attcgccagg agaatgatta acccaacaac agcaaagaaa
    37441 accaggcaca ctccccaatt agctcgtaag gccctcttcg acgttataga tataaactca
    37501 aagtcccttt tagatctttt ttcggggagt gggataatat cttttgaagc cattagcagg
    37561 ggtgcagcca acgtcttggc agttgagatt tcaagaaggg cctgcaattc aattcttgga
    37621 aacaaacggg gaattgatcc ctctatggat ctggagattc tctgctcaga ttttcgtagg
    37681 gcaattccat cattcgtaaa aagcggaatc gcatttgacg tcgtatttgc ggaccctccc
    37741 ttcgatgagg aatacatcca gcctcttatg gcagttcttg aaagaaatac cgagcttcta
    37801 aaccttgccg gaatgcttat agtcgaatct tctgtaagag agaagggttt tgtcttgcaa
    37861 ggtgcaggat ccttcgatct tgttgaccag agaaactatg caggagtagt gttctctttt
    37921 atggtgaaga aatgagattt ttggttgaag agcccttaac caaactagcg aggaagctca
    37981 gactgatggg ttttgacacg gaaatatgct ctagaaatga gataggcaag tcatttgaga
    38041 atcgggatgt gtttttgact aagagtaaga aagcgatgga gatcgcgatt aaatttggga
    38101 tcagatctta tttaatcaag tcggagaact ggcgctcgca gctcaaatct gtaattcaaa
    38161 gattccagat aggtttggac gatgttcact ttcttagcag atgctcgaaa tgcaacggtc
    38221 tgcttgaaga ggcttcccta aaagatgttg caatggttcc agattatgtg gtgcttacgg
    38281 cagaaaggct ttacaaatgc accaggtgtg gccagcttta ctggaatggg agtcatgttc
    38341 aacacatgaa atggatgaac gaaattgcag gaaagagtgg tggtaatgct gaagaaaaca
    38401 aattttgaga aatcatccga tgaagtaatc cagaggattt tgaagaagat ttcttctgaa
    38461 ccctcttcaa aagcatcgct gttcatactg gagaaatatc gaaacgtttt caaagaagtc
    38521 aacccagatg aagccttcac tcgcactggt agagagataa ggttaagcct aaatccctct
    38581 ctgaaggagc tttctgagga tttttcggga gagatgaacg ttgttgtttc ctctcatggc
    38641 aaagaattca gagaaaaagt atttctcgtc agtctttctt acgatggaga acctcatggg
    38701 ctactgatta tgcctgcagg cgcagaatcg aataaatcac tcaaggactg gataaaggaa
    38761 attgagcttg ctttgcatta ttcgctgaaa agatcagccg aagaggaagc tttcagacga
    38821 ttgaaactgg tttcaaagct tacttcggta ctggaaaccg aaactcaagt tgaagaattg
    38881 atacgtaaat cattggagat agcaagagaa atgttgagag ttagatggat cttctatctc
    38941 gaagggcagg gaaagagtct tcacctgaag gcggctgtgg gaaacggtaa taaccctctc
    39001 aagggaatcg aaatagagat ttctgatgat cagttgagtg aactggcaag tgagaagctt
    39061 ttcgtttctg cgagaaaatc agatctctca tctgcaatct ttaaagaaga tgtttctgtg
    39121 ggctccttca tggctattcc tctagcagta gaaggaacac taaacggact gatcgtcgct
    39181 gcagacaggg aagatattga aggagaattc agagcatata aacatctcga caaagaagat
    39241 cttaaaattc ttgatgatat aagcagaaga atggccgttg ctattagcag aataagactt
    39301 acaaagaggt tggagctaga agtaaggaag ctaagacagc ttgggaagaa acatgaagaa
    39361 ctcataaatg atcaaaaaga tcagcttttc aagcttggag ttctccacaa aattaccgag
    39421 gccatgaaaa gaagcatgga cagagagaga atagttagaa ttcttctaat aggcgcgact
    39481 gattcctcag gactgagatt cgatcgcgcc acatacttcc gtgtagatag cggtcgttcc
    39541 cttctcattt tggaaaccca agtaaattct acgaggggca atagtttcaa ggatagatcc
    39601 gcatacagtg atcttacaag gtatcttcaa gatgtttcgc ttgaggcggc acaaagtacc
    39661 actaataatg agccttcggc atcaatttca tatcctggga atatcattct agaaagggtc
    39721 atcctcagga aaaaaatggt tcacgtttct ccagaaatgc tcaggctcag gggagaagag
    39781 ctgagcttga tgaacagaat tatcggcact gacgagtatc tcattgctcc cgtgtcaggg
    39841 gaagaagaag tacaaggtgt actggtggtt gacaatgctt attcccggaa gaaaatcagc
    39901 agtggtgaca tagagattct cggattgctg gccgacagta caggagtagc tcttgaactt
    39961 acggagaact ataagagaat cgtacagatg gcagagagtc tcgaaaaaga gagaaatctt
    40021 tccaattatt atcgaagatt cgtttcaagt atacttcagt ctctagaatc agcaatcata
    40081 gtttgttctg ttgacggtga aataacggaa atcaacagaa ctgctggatt tcttttgaac
    40141 attgaaagag aagaattgat tggaaagaag attcaatcaa tgaaaccaca acttggaagc
    40201 attatcgacc ttttgtcgga tgcgatgaga attggcgaga ctattaccct aacggatcaa
    40261 tactttgaag cctttggaga aagatacttc gacgttcgca taacaccgct gagagaagag
    40321 aaaaggaaag gtctaactgg cgtgattctc tccttagatg acataactag gaggagaaat
    40381 cttgagcagg aactgaaggg tagggaaaaa cttgcggctc tgggggaaat gtctgcaaga
    40441 gtcgctcatg agattagaaa tccaataaca ataatcggcg gcttcgtgaa aaggattctg
    40501 gcttcctcag acaggaagaa aaccgaagaa tatgcgaaaa ttctcatgga tgagcttcaa
    40561 agactggaca gaatagttgg cgaagtactt gaggtttcta gaagaaaggc caaaccatct
    40621 aaggaagcgt ttgacctggt ttctatgaca cgtgaaatac tggctggatt cgaagaaaaa
    40681 gccgcgtcgg aaaatgttaa acttgttcta tatacgaaag aagaaagaat tgaatattac
    40741 ggaaatagag ataaaatcaa acaggtaata atcaatcttg ttcagaatgc tattgaggcc
    40801 tccgaatctg gagatgaggt tcatgtaact cttgagaaag atagcgagga aatccgtttt
    40861 tcagcctgga acggtgggaa aactatacca gaagagactt tgaagcgcat ttttgaacca
    40921 ttcttcacta caaagactct cggtactggt ctaggacttt ctatttgcaa aaagattgta
    40981 gaagagcatg gaggaagcat tcatgcttca agcggaagtg aaggaaccgt ctttactttc
    41041 aggttgccga ctaaggaatt tgggaggttt gatcttgaaa agagtactga tagttgaaga
    41101 tgaaaagaat ctgaggctac taatcgaaga agaacttacg gaatctggct ttgaagtcaa
    41161 gagtactggc acagctgagg aagctcttga aattctcagc acaaataact cgtttgacct
    41221 catgaccatt gacattgaaa tgaaaggaat gaacgggctc gagcttgccg gtgttgtgcg
    41281 aaagcggttt cctaatatga aaattgtgct tttaaccgct tatactcatt acaagcacga
    41341 tctttcttct tgggccgccg atgcatatgt tgtcaagtca ccggatttct cagaacttaa
    41401 gaaagttata gaaagccttc tgagagaaga ccttgactag taaagaagga ggaaggaaga
    41461 tgcacaagaa aatcatatct ctttcaatag tgcttttgat tacaatgtca cttctaggat
    41521 attcagtaag accgggtgac cttctatcga tttccgtata tgggtccagc gacctttcga
    41581 gtgaaaccct tgtgggaccg gatggaactg tttccgttcc tccgcttgga agcattatgc
    41641 ttctgggaaa gacactagag gagattcaat cctttctgtc ttcacaattt cttagcaagg
    41701 gcattcttac atcagagccc caggtaactg tatctgtgaa gtcgtttgct ccattcatgt
    41761 tctacgttct gggagaagtt aacaaacccg gagcaattga gtggcgcgac gaaactattg
    41821 ggatctctca actgcttgct cttgcgggcg gcctcaaaga cggggcagat ctttcctcat
    41881 ctttcatagt ggatagcgaa ggaacaagaa attacataga tctatcctca attctctccg
    41941 gtggatccgc tctaaaagag aaaatctctg agggctcaac attattcgtt ccaacaggtc
    42001 ctcgtgcgtg gataatgacg atgggcgagt tcaattctcc ttcactagtc aaatacagcc
    42061 ctggaattac tttgactcag gtgatcgcca aatctggagg actaaaggaa accgccgatg
    42121 ataacgaaat catgattctc agcgaagcta aagagaattc ttcgatcgtt agtctgaaca
    42181 gcgtcttatc cggcataaaa agtgaccctg tgctatctgc tggaagcatc gtcatagcaa
    42241 acgatgcctc atcaaaatca gtcaaacttc ttggagagtt tggcaatcct tcatcaatac
    42301 cataccgtga aaagctgact ttgcttcagg ctattggaga ggccggagga gtaaatcagg
    42361 ccgcttcgga ccagctttac attctgagga aagacctaga tgaaccggaa tccgttgaaa
    42421 tcagcaaact cctcagcggt cagatcaaag atatagacct ctacccaggt gaaacagtat
    42481 tcgttccacg ccagcctgaa agatatgtat atatagcctc cgcatcgttt ggaggaaaag
    42541 ttgaattcag tatagatgaa gaaattacgc taatcaatgc cttagtaaag gtagatctct
    42601 ttgatccagt aagccaggac ccattagttg ttgtaaccac atcaggcgaa cgtcgtgagt
    42661 ttcatcaagg ggaagatgcc gttctcgatg caggatcgat gataatactt ccaggcggtg
    42721 aaaagagtat cttcgtcacc ggtgaagtga aaaacccggg cgtcctttcc ttctcgaatt
    42781 tcgaagaaat cactcttgga aaagcaattg caaaatcagg tggagtaacg gagtttgctg
    42841 gctctgtaga acttctggaa aatggggagg caaaaaagtt ttcaatcgaa gatgctgtta
    42901 agagcacttt ggttctttca gctcaatcta tcgtaaatgt agcagagaaa gagcgtcgtt
    42961 ttgtatacgt cgtttcttcg gcagaaggtg gaagaatcga ttttgaagaa agagaaccat
    43021 tcactcttag aaacctgctg gcaaagatga atttactaaa cttcaattcg aagaaggaaa
    43081 tatcgattat tgacaccgct ggaatgagca aatttctttc tgtttctacc ctatcaaagg
    43141 aagactatca gcttgagacc ggttcaatcg tgttaattcc tgatataagt acatggatat
    43201 atgtatttgg ggaagtggaa agacccggaa agatcaagct cgatgaagac gatcttcttc
    43261 tgacgcgtgc tatcagtgca agtggaggat tcactcaaaa cgcagacatt tcgtccgttc
    43321 aggtgctttc tggatcggaa atcagtgaaa tagatcttga tgaagttctg aagggaatat
    43381 ccagtgatcg ggaaatttct tacgacgatc tcatatatgt cccaagaatc gacagtcgat
    43441 tcgtctacgt tgtttctgag gaaaatggtg gaagagttga tttctcttct ggcgaaagca
    43501 ttaccctcag aaatgccctt gccaaactaa atcttgtgga gatcgcttca cagaaggaaa
    43561 tttcggtgat ttcaccgtct ggtaaggaga taagagtttc aattagttct cttgaaagat
    43621 ctgacctcga actggaaacc ggaagtgtaa tattttatcc tcaggcggat aacgtggcaa
    43681 atgttattgg acaggtaaga aatcccggta ctgtgaatct taaacccggt atccccttca
    43741 atctgagcac cgtaatagca gcagctggtg gcataaccga cctgggcgac gaattcaata
    43801 ttacagtaat agattctaag tctgcttcta cagctgttta tagcctcgaa gatctaacaa
    43861 gagacccgat tccggtaatt ccagatggag ctactgtgtt tattcccgaa ctcgaggcga
    43921 gattcgttta tgttttgggg ggcgtacgag agcctggaat aaaggaaata ggcaaagaag
    43981 agccaaatcc aactctgaca aaactaatag cgatttctgg tggattaagc gacccacttt
    44041 cgaaggaagt tgagataatt gaggataatc agagaagaaa gcttgaccta cagtcagtaa
    44101 tgatgggtcg tattcaagat ccacaggtat cgaatcattc gataatatat atttctgaaa
    44161 ctcccgatcg attcgtgtat gtaatctcta aagagaaggg cggaagagtc gactttggaa
    44221 gcaatgagga aatcacccta aggactgcac tggcaaaaca gaatcaactt gattttgatt
    44281 cggatggcag ggttaccatt atccttcctg acgggacaaa gagagagaga cagatttcag
    44341 atcttgggga gagcgatatg aacttatccc ctggatcaat agtaatttac ccagatgttc
    44401 tacgtcaggt gtttgtggtg ggtgcagtta acaaaccggg actcgtggaa ttcgaacctg
    44461 gagaaaaagt ctccctaaca actgttctcg caagggcagg tggaatgacc ccgcttgcgc
    44521 tgagtagcaa gatacagata acggatcctt ttggcaggac ctcaactgta agtgtagact
    44581 caatacttct tggtaaggct cttgacatga atcttgaaga tggttctttc gtttacgtac
    44641 ccatatatga accagtcagg gtaaatgtaa taggagaagt caagtatcca ggaacgattg
    44701 agttttctaa gaccgaattg ccatctctta tactcgctat ttctagagcc ggtggtttga
    44761 ctgaaaaagc tgcacaaaac gtgaagatca cgggcgaaga gaaagaagta ctgtggctag
    44821 atcttgtcga tggaaaagat gtgatgttag tctcgggtca aaccgtctac gttccgggtg
    44881 acgatcgata catctacgtt ggcggtgaag taaactctcc cggaaaattc gagttcgcag
    44941 ctcaggagga gattacggtt ctgagagctc tcgcaagggc tggcggtgaa aaggccaccg
    45001 cctcagacct ggtcaaggta ttgctgccct caggggagat tctaccagtg agtctttcga
    45061 atatcaggaa cgaaggcaag gatgcggtta ttgaacctgg aagcacactc atagtaccaa
    45121 aacaagtgct aagaataacc gttcttgggg cagttaagaa tccaggtctt ttcactttca
    45181 atccagatga ggatcactca ctttccgatg ttatagctag ggccggaggt ctcatagatg
    45241 aaagtcttgt tgatgcgata attgttcatg acgacaaacg atacaatgaa tatactccag
    45301 aagttttgca gaaaagagga aatcaactgt ctgataacac ttatatctat gtgtccagta
    45361 gatcacccgt ctctgtgact gtcctgggtg acgtcgctag acctggagag tatgaaatca
    45421 gcgtcaggaa cccttccatt gcaacggcaa tcgcagttgc cggcggagtt gaaaaggccg
    45481 taacaacgct aagcctaatc ggaatagatg gcagggtgca attgtttgat gccttgagga
    45541 ccgaggaact cgcaagaagc tatctagaag atggagatac cattgtcgca ttaagaaact
    45601 acgattcttt tgtcgcggtg gtcggtgatg tggaatcgcc gggagtattt ccagtctcga
    45661 actatggtga gctgactctg gctgaactca taagtcttgc tggaggaatt acaaatttcg
    45721 aaagcggcgg aagagtcttc atatcttcag gcggcaagac ggaagagatc attatttcac
    45781 ctgacgattt catcgctctc accagaaaag tagttcccgc tggatccact gtatatgtac
    45841 cttacacgga accggccaga gtttatgttt tcggtgaggt tcagaaaccc ggtatcgtta
    45901 gatactatga cggcatgaca gccattgaag cacttgtaga ggccggggga ccgacaagct
    45961 atgctgtgct cggaaatgca ttgctcttcc aggatcttga agaagaacca atggtaatcg
    46021 acctggatca gcagaaagga aaacctgcga aggggaatat cccgctgata ccgggaaata
    46081 taatcttcgt tccgcagtcc ccaatagtga atattaaaga tattatgtcg attgttgcgt
    46141 catcgctaag catagtgaat tcagcagttg gcatatttaa gtgaggtttg aggaacttga
    46201 atattgactc tcattgccat ttgcttcccg cagtggatga tggtgtggag gaaatcgagg
    46261 aatcattagc aatcctggaa gaaatgaaac ttaaggggct gcaaaggctt tatttgacgc
    46321 cccatttgtt ctctcccata agtccttcag atcctgcgga aataatgagg cgatggcatg
    46381 aattcagatt cgaactgaat aatcattcgg tcgaagttct gcttggttcg gaaatctttc
    46441 ttaggcctga ggtgttagaa tctgatttga tcacaatggg tgagtccaat tttctgctag
    46501 tagagctctc gactcagcag aaaccacatt acctttttga agtcatagga aagcttcaaa
    46561 gtacggggtt tagaatcata ctcgctcatt tggaaagata tcattatctt ttcaaaaaga
    46621 taggatttat ctttgggaag gtagaaccaa cagacgaaat acacaggcta agagacatgg
    46681 gagttctatt tcaggttaac tggaacagcc ttgataagga ttcaaaggca agagttcttg
    46741 ttgatcggag gattgcggat attactggaa gcgataagca caaacaaggc gataacagac
    46801 tgcttatcga cttcgatgat gatcgataca gactttttct gaacgattct tttctctaga
    46861 tggaggtgct ttagtgagcg agttcgaggg tagtgattac agagaactga ctcttgaaga
    46921 tattcttaga atgtttaaga aacggatgat ccttttcgtt tctatagtgc tcgcagttgt
    46981 tgttgtgacg ggaatctacc tcattttcgc tactcctatt tacgaagcca gcgttacaat
    47041 aaaggtcgac cccacatcac aatcttcagt aagtgatctc ttcagtagtt ccctgaccgg
    47101 tagttcggga tcgaacatct ccactgaagt tgaactgatt aagagccgga caaacattga
    47161 agagattatt gaagtgcttg atttggtgga cagaatctat tcagaagaga caaagaaccg
    47221 acttcttgcg gaaggatatt cacagaaaga tcttgtgaca tcactaacac gcacgatttc
    47281 ttcaatgata acggtctctc cagtcaagga tacaagaatt gtcagagtct cggttcagaa
    47341 cagggaccca attctcgcaa gagatatcgc caatacacta gccgatgttt ataatacaaa
    47401 gctcgcagag ttatccaaaa gagatttgac aagaaaacga gaattcatcg aggcgcagat
    47461 tcctctacta gaaagtgatc tgaatgaggc tacagatagg ataaaggatt tcaaagagga
    47521 gaccggaatt tacgttctcg acaagcatgc cgatcttctt tttcagatgc ttagtagtta
    47581 tgacaagcag tacaacgaac ttatgatatc cgctgaagag aagaaagcgg aaatcgagac
    47641 ctatcaaagt atgctcgatg atttcgacag caatgattcg aaaagcataa aggctttgtg
    47701 ggtgcaaacc tccgagagct tctctgttaa cccagttctg acgagtctga ggcaaagtct
    47761 cgcaactttg caggtggagc ttgcttctct agaggaacag tatccgaaaa ccgatccaag
    47821 agttaggtcg aagataacag agatctcaaa gaccgagagc ttgatcgagg aacagataca
    47881 gaatgagttc ataacctccg gtcaaggtat gactttaaat ccagcttatc agcagattat
    47941 gacaggagtc atcagttcgg aagccgggtt ccaaatactt caagcttcaa ttcaggcggt
    48001 gggtctcctg agagatcaat atcaatctga actcagaagc ctacctgcaa aggaacagca
    48061 gctacttgat cttgaaagac agattgctgt gaaagagagc ctctatacac tacttctaga
    48121 aaggctcgaa gaagcgaaga tcagtgaagc tgccgtagtt ggaaacgcag caatagttga
    48181 tcccgcaact gtaccccagt ctgcggtaaa gccgaacaaa aaactttctc tcgcaattgg
    48241 aggtgtgctt ggaatctttc tcggaatgct catggtcttc cttgctgagt acctcgacaa
    48301 gactttgaag acggaagaag agatagaacg gtttagcaga cagcctatca ttggtagaat
    48361 tccgaatatt gagggggcaa gcgaggaaat gtacgttgag aagaatccca ccgctccttc
    48421 ctcagaatca atcaagttgg ccgcaagcaa cctttccttc acgatgggtg agggaaagtc
    48481 tgttgccgtt acttccgttc tgcctaccga gggaaagagt tttgtgatag cgaacctggc
    48541 ctactcaatg gcaaacagcg gtcagagagt cgttctctta gatctcgatt tgcgtcgtcc
    48601 gagggtagag aagatcctca aggcagaaaa gataactaag ggcgcagtag atgtgattat
    48661 gggaaacaca acaattgatg aagttataca acactacgcg gttaacatgg acttcatagc
    48721 ggttggaact attcctccca atccaacaat agtgctttca tcgaaaaaca tcgatgccct
    48781 cctttcagaa ctcaagcaga gatacgataa agttcttata gatatgcctc cggctgtggt
    48841 tacctcagat gtatctctca tcggtaacaa gcttgatggg atagtactgg tcgtgaggcc
    48901 tggaagggcg attaaggatg gcttgagaat agttgttgag aatctaaaaa cagtgggaat
    48961 caagatcctt ggagtaattg tgaatggtgt tgatgagaag aattcaagct attactatca
    49021 ctactactac tattacaatg aagagggaaa gaggcaaaaa agaagaaagc gtacaaagaa
    49081 ataatcaaaa tagctaatac cgaaaattcg aaaagttcgc tactgaatgc tgacgaaact
    49141 aataatggca attctctgcg tttttagtcc tgaactcttt gctttgacga ggtggtttgg
    49201 atgtccgtaa agaagcacgt tatagacttc gagacaattg tctatctgtt tttaacgctg
    49261 ttcatccctt tgtttgtcac aaagggattc acacatgagc cttcaactgg aaaacatctt
    49321 ttctacgtta ttggattcac aattatcttt ctttctgtgt taataaggaa aaaagagatt
    49381 tcagtagaat ttggagacgt tcatctggca tttttcggaa taggcatagc ggctcttttg
    49441 tcattgatcg tggtctctat ggacaatcca cagtacctga gatattcttt agaaatcgct
    49501 ctctatgtag tttttctctc tttcacagcg gtctatattt cgaacaaatg gaacaccatc
    49561 gagaagctag aaattgtaat gatctttttt gtaattggag ctgcggtagt tgcttttgac
    49621 gctctcctga acttctatct aggtgtggac atctttcttg gtaaggtcgg cgagccgttt
    49681 gcaagagctt ctgcaagatc aacgattggg aacccgaatt tcgtgtctga ttatatggga
    49741 atgaccattc caatgatatt ctacttcctt atatcgagaa aaccgctagg ctttcttctc
    49801 aaaagacatt ttggccagct agttttcaag attactttgc tcatttttct gatacccatg
    49861 gtctcttccg tattcgtgtc acaaacaaga acggtgataa cagcaatatt cgtcggaaac
    49921 ctgctttttc ttgttcttta tttctttctg aaaaagagaa agaagaagga agccctagaa
    49981 gactctgaga gcagaaaact gggcagagtg tctctcgttt tctttgtctt agctctgata
    50041 atcatcgcag tactctctta tctatacctc accccttcgc cactgtccgg tgatggaaga
    50101 atcaacataa ccgcaagact ggaatatgct atcacttctt cgggttcctg gaatgaaagg
    50161 ttttctgctt ggtacaattc gatttttcag tggctggaaa gtgagaacaa gttgagaatt
    50221 ccatttggct cgggcattgg cacttttcaa ctctatcatt tgctgtacag tccagatgtt
    50281 cttcagcata gtccttactt tatgccagtt tggaataatt tcaaaaggac gcacaacgac
    50341 tacgtacagg gcctgggaga aatgggtcta attggatttc tatttatcgt tcttctcgtt
    50401 ggattgctcg tttttagata tttgaagaat ctacttaaga tcgacaatag aagggactta
    50461 ctgctttacg gatcgctggg ggcaggaata ttctctctgg cagttcacag tttctttgag
    50521 ttccccctac acatgcaacc aaatctcatg ctggcaatct ttttgggatc agttgttgtt
    50581 ggaaaatact tcaatccaga tctaaaaaac aagaaaatca gtagactccc gtttgcagta
    50641 gtcattatgg ttcttgcagg ggtcctgata tttctgaaaa cctcagcctt tataggggaa
    50701 ggctttttta ggatcggcca gactaatcag cagtattatc tcgcttacta caaccaggct
    50761 caaagcctca atattaaggc tctcgaacaa gcaaagaatg aaatcaatag ttacgctggc
    50821 aattatgctt atcttaaaga cgttaccagc tacatgagcg ccaaaggaag tgagattcgt
    50881 tcgaagtatc ctggagcgag ccaaatagac cttcttgagt tggccgagaa agatcggcag
    50941 agtgaggtgc gcaaattact cgacgaaata gataatcgaa ttaaccaata taacttctac
    51001 ttggcgagat caggggagta ttatgaaaag gccattgaga atttcaagtt ctcaaacaga
    51061 gtttatccgg tcttcggaaa gccattatgg tacatcgccg ggctaggtac aaaaacacag
    51121 agacttgaaa ctgccaaaga caacccggaa ctcatgaagt ctattctcac aggcaaggat
    51181 gaatactcat ccgatataat cccggagttc aagggcaacc ccaaaataat ccccgttcac
    51241 aggacgacga ttcggactct tcctttccgc gatttcttcg agaaaaacat ttcggtcttt
    51301 gacaatcccg aatacgtttc gggattgcag ctgtatttca ttactcagat tcagatgatt
    51361 ctggacgcgg ccgattatta cgaatcgtca gtaatacttt tcagcgaacg acaaacacct
    51421 agaattcttg ggaggttgta tactagcata aacagcgagt taaaaaaata ctacagtttc
    51481 atcagttcta gagagaccat gatgacctcc gcctttggag agagcggtga attcagacag
    51541 ttactcattg atctggtata cgaaagtgct gatagagcaa tatactggtt tgatctggca
    51601 atttcgcttc ttccgggcac atggaataga tacccagact gggaggatat ttatattgaa
    51661 tacatgaact cgattccctc tgttctcgat tccaatgaag agagaaagct aaagattctt
    51721 gaagttgccg agaaacatgt ttgggcctgc gagaacatgg gcccagaggc tccagtagaa
    51781 acgctgcagt ttgcaatcca atggggcaaa tccaacctgt ccaatggcga attgatagac
    51841 ttcgaacaat ctttgaaaaa gatctatgaa gaggttgttt ctctaaacac tgacttgctg
    51901 gagaagtcac caaatattcc tcaggataca gccgaaagaa taaggactct tatctcgctt
    51961 tacgaagcgc tttgaattgt ttcgtttggt atacatttag ctaagtaaat gatataatat
    52021 ttctggaggt gtcctgtgga gaagttctac gtcactacgc caatatacta catcaacagc
    52081 gaaccgcata tagggtccgc gtattcgaca atagttgcag atgttatcgc aagatatagg
    52141 agactctcgg gatacgatgt gtttttccta actggaaccg atgaacatgg acagaaggtg
    52201 cttaacgaag ccatagaaag aaatgttgaa gcccagaaat actgcgatga gatggctgaa
    52261 aaattcaagg cactttggaa agaacttgag cttacaaacg acgcttttgt aagaacgact
    52321 gatgaatacc acatgaagac tgttcagacg ttcgtctcca agctaaagga aaatggcgac
    52381 gtatataagg gaaagtatga gggctggtat tgcataccgg acgaaacttt ctggaacgaa
    52441 gaagatctta aggaaggaag aacttgcccg gagtgtggac gagaagttaa gtgggtaagc
    52501 gaagaaaact acttcttccg cctttcaaaa tataatgata ttcttctgag gttttacaaa
    52561 gagaatcccc agttcgttca acctgacttc agaagaaacg agatgctaag gattttggaa
    52621 tccggattga aagacttgag tattaccagg acttcatttg actggggcgt tccaatgcca
    52681 gatgatccag aacatgtcat atatgtttgg gttgacgcgc tgataaacta cttgagcgct
    52741 attgggtacc ctcataacat tgatcatttc gaaagatact ggcctgccga tgttcatctg
    52801 atcggcaagg agataaatcg ttttcactcg ataatttggc ccgccatgct tctctctgca
    52861 ggtcttcctc tgccaaaaca gatctatgcc catggatggt tgaccgtgaa tggccagaaa
    52921 atttcaaagt cactcggaaa tgcaatcagt cctgtggcct tcgttagaac ctacggcaac
    52981 gatgctttga ggtactacct cctaagggac atacagtttg ggagagacgg tgacttctct
    53041 gaaaccaact tgattagcag aatcaatgcc gatcttgcga atgatctcgg taaccttctg
    53101 catcgaactg tcgtcatgat tcagaaattc aacgatggtg ctgttcctga aaggagcatc
    53161 acagaaacga tagacgaaga ccttgtggag cttggggaga agacattctc tgcctacact
    53221 gagtcaatgg ataatatgag gttcacccag gctcttgaat cagtttggga gctaatcaga
    53281 ttcggaaaca aatatatcga tcttactgag ccctggaaaa tggggaaaaa accggaaatg
    53341 aagggcagac tagatacggt tctgcacaat cttgtctgca ctttgaaaaa tgtttccata
    53401 atgattgagc ctgtaatgtt tagaacagcg agagagattc gaaagaggtt aggttttggt
    53461 ggaaccatga ccctcgaaaa cgtaagctgg tcggatatag tggcaggaaa tagagttagt
    53521 cacggagagc cgatttttcc tagaatcgaa atcgaaaagc acaggaaggt gactgagatg
    53581 gaaaacaagg agacgggccc ggagatcaaa gacaatcttt taataagcat tgatgacttc
    53641 tcaaaagtcg aattgagggt cgcatctgta actgaagccg aaatcgtccc aaaatcgaga
    53701 aaactcgtaa aattacaact cgacctaggt gatctaggaa ggcgacaggt agtagctgga
    53761 attgctcagt tctataaccc tgaagaactt gtcggtctta aagttgtcat cgttgcgaat
    53821 cttcaaccgg caaaacttat gggaattgaa tcaaacggca tgatcctggc tgcaaagatc
    53881 ggtgattctc tttcactgct cacggtacac aaggatgtgc aaccaggcgc aaaagtttct
    53941 tgacaaccaa ggaggaaaaa gtctttgccc gaagagataa tacacaagaa tatagatgat
    54001 gaactgatta attcatatat gctctactcg atgagcgtca ttgtgggaag ggccattcca
    54061 gatttgcgtg acggtctaaa acctgtacaa cgcaggatac tatttggaat gtcggagcta
    54121 ggtcttcatc ataaccaagg gttcaagaaa agcgctcgaa tagttggtga ggtcatgggg
    54181 aagtttcatc cacacggcga tgctgcgata tacgacgcgc ttgtcagaat ggctcaaccc
    54241 ttctcaatgc gctatcctct cgttgaaggc cagggtaact ttggttcgat agacagggat
    54301 ccgcccgcgg caatgaggta taccgaggcg agaatggaaa cgcttgccga ggaattacta
    54361 caagatatcg ataagaacac tgttcccatg atgcccaatt tcgacggatc gctatctgaa
    54421 cctgacgtac tccctacaaa gattcccaac ctccttctca atggcacatc cgggattgca
    54481 gtgggtatga tgaccagtat tccaccccac aatcttgtgg aaattgtcga tgctatcagt
    54541 ttgttgataa aagatcccga atgttctgta tcggatttac taagacacat aaagggtccg
    54601 gacttcccca ctggcggtat gattatggat gccgcttcca ttccctcaat ctatcaagaa
    54661 gggaagggaa ggataaccgt cagggcaatc gcagagattg aggaatcggg aggaagctca
    54721 caaatagttg tcagtgaaat accctacaat acatcgaagg cggatattat agaacagatt
    54781 gtgaatgcaa ctcagaacca tcgtgatctg caaatcagaa atatcaggga tgaatcggat
    54841 caaaaagggc tcagagtggt cattgaactt aaacgtggag cagatccaaa tgtagttctg
    54901 aatctgcttt ttaaacacac gcaattacaa acaaccttta gcgtcaatat gctggttata
    54961 gacgaaaaaa agcgaccgag agtaatgaac ttaaaacaga tccttcaggg atttctttcc
    55021 cacaggttca acgtaatcag atcgaaaacg gagtacgatt tagaacaagc atcgaagaga
    55081 gcacatatcg ttgagggatt gacaaaggcc acgagatcga tagatactgt tgtggatata
    55141 ataagaaatt ctgccaatag cgaagaggct tccaaaaacc ttatggagac gctggaagtc
    55201 acccaggagc agagctcggc gattctcgat atgagaatgg gaaagcttac aggaatggaa
    55261 attgaaaagc ttctcaacga gtacaaagac cttgttgcaa aaataaacga gtacagaatg
    55321 attctcgcaa gcgatgagaa ggtttatggg ataattctag atgaattgaa tgaaataaaa
    55381 gcgaagtacg gagatgctag gcgtacaagg attgatttgg gaaatggaac tgatttcaat
    55441 atagaagatg tgataccgga cgaggatata gtcctgatgg ttacgaagaa gggctacata
    55501 aaatcgactc ctctcgaaag ctacagaaaa cagggtagag gcggcaaggg cgtgatagga
    55561 gtaagaaccg gagacgaaga cttcgttgtc aatatactta ccaccactag actcagcaaa
    55621 acggtcataa taacatcaaa aggaaaggcc tacgtgttga gcaatcacat catcgatcac
    55681 tcgtctagag attctaaggg aaagcttctt gcaaactaca tcaagatcga tccagatgaa
    55741 aacgtgcaag ccgttctttc gacgagaaag gaagaagttc aaggaaagca acttgtcatc
    55801 acgacaagat cagggaagat aaaaaagact gagttcgaag catttttgaa tgtacgaatc
    55861 tccggcataa aggctattag cctcaacgag ggtgacagag ttatcgatgc atcgcttaca
    55921 tctagagaag atgacaccat aatcatctca acaaagaacg gaatggtaat aagattcccg
    55981 ctggatcagg tacgttccat gggaagaagc gcagcaggag ttatgggaat caggctgaag
    56041 aagaacgatg aagttgttgg cgccaacatc gttgcttccg aagatacgag attcctactc
    56101 acagccaccg aaaaaggtgg tgggaaaagg acctgcttat ctgagtacag gagtcagaat
    56161 cgtggaggtt taggagtaaa aaacatttac gggctggaga agatcgggaa agttattgga
    56221 acgctagtag ttaacagtga cgatgaagtt atacttgcta ctaagttggg tatgtctatc
    56281 agaatccccg tatctcaggt aaggcccact ggcagagtaa caaagggagt cagaatagtt
    56341 gatctgaagg ataacgatat tgctgcatcg atggcggttg ttgttgacta aaaatcccag
    56401 tcaaacatag gggggtgtca tgaattgact ctgacggaga aacaaaagaa gattcttgag
    56461 tacatcgaga acttcattaa gctctatggt tacccgccaa gcataaggga tatctgcagg
    56521 gatttcgaga tatcctctcc aaggggggtg gcgaaacatc tcgagtctct tgaaaaaaag
    56581 ggttacatcg aaaggaccgg cgtttctcga ggaattcgag tcttgagaag cccagatggt
    56641 tcgtcgcttg aacccggtga agatgttgtg atgctcccag ttattggcaa tgttgcggca
    56701 ggtgaagcta ttcaagcggt tcaaactgaa gacgagaaga ttcccgtgcc gctttggatg
    56761 atcagaaggg gattcgaata ctacatgctc aaggtaactg gtaacagcat gattgaatct
    56821 cacatagtca acggagattt cgttatcata aggaagcagg agtgggcaaa caacggtgaa
    56881 atagtggtag cactgataga tgatgaatac gccactctga agaagtacga aaacgaaggg
    56941 ccgaaaatcc ggctcgttcc ctcaaatcca gaaatgcttc caattgtcgt tgaagctaac
    57001 agagttaaga tacaaggcag actttccgga gtgctgcgtt ggtataaata gtagaacgct
    57061 ttttcaagga gttgaaacat gaagattgca attggttccg atcacgcagc ttatgaactc
    57121 aaatcttttc tcgtgcaata tgtcgagagt aagggccatt cagtaattga tcatggtcca
    57181 gaatcgggca gtctttcggt agactatcct gattttgcag acaaggtttg taaatcagta
    57241 actgacaatg atgccgatta tggtattctg ctttgcggaa ctggaattgg aatgtccata
    57301 gcggccaaca agcacagagg cattcgtgct gctctctgcc ttttccccga gatggcggct
    57361 ctttccagga gacataatca tgcaaacgtt ctagtgcttg gaggacggct gatcggaagt
    57421 gagctggcgg gctggatagt cgatgctttt cttaattcgt ctgaagaagg tggtcggcac
    57481 caaaccagag ttgacaaact tgaaacggat actttcgaga atcgggtgaa tgagccttga
    57541 tagttttcta cgacagaagg catctcttcc atctcccgat gaaggagctt gataacggta
    57601 actggattga aaacccggac agaccagaaa gaatcgaatc tgtccgtagc gctctagaat
    57661 ctgccgggtt tcaaataaaa gaaccaagag attaccactg ttcccatctt tttcaggttc
    57721 atacgccaga atacgtagaa tggctcaagg cgaaaagtaa ttcggtttcc agagacagag
    57781 aatattttcc agaggttttc ggctatgata agctcttcga tactggtaca ccggtcactt
    57841 cgggatgcta tgtgagtgct ctcgcatcag tgtcaacagc gctgaatgca gttgactcta
    57901 ttcttgaaag tgaacccgtt tcctatgcct tatgcaggcc accaggacat catgcaggca
    57961 aatcgactgg tggaggttac tgctacttca acaacgcagc aatagcagcc aagtactacc
    58021 agaaacacac caggggttat gttgcaattc tcgatttaga ctttcaccat gggaacggta
    58081 ctcaagaaat cttctactac gatgatactg ttttctatgt ctctatacat ggtgatccta
    58141 agaatttcta tccctggatt agcgggaact cctgggagat aggttctgac tcaggagagg
    58201 gatttaacat gaattttcct cttgccggag gtatagaagg cccagagtac atgagaactc
    58261 tgtcgaaggc tctgcaagag atcgaagact tttctcctga tctcatgatc atctccctgg
    58321 gtacagacgg tcacaaagat gatgacatag gccatttcaa tctcagcagc gaggattatt
    58381 caatgatggg aaagttgatc ggggaaatag aagttcagaa gcttatagtt caagaaggcg
    58441 gatataatcc caaagcaaat tctgcctccg ttttgaactt ccttacgtca atcagatgaa
    58501 aatctaaaga aagcgcggcc aggccgcgct ttctttattt aatcaaaatg ctgctccaaa
    58561 accaaagaac ggcgttccat aatacggttt ccctgctcca gagttttgct cagcaagaaa
    58621 agtgttgtaa ccgtagccaa gtcggaccaa acctatcatc ggtatcgata tgtctatgca
    58681 cagaccaaag tcagcaagga aattccctcc tattaagtct ccgcctggtg tctttctata
    58741 gaaaagcaag tctccaaatc ccacaatatc taagggtatt gaagaggcct caggtgttag
    58801 gggatacctt aactgcgcag aacctacggt aactccgtac gcgttctggc ctataatttg
    58861 cgaacctctg actcgaacaa atgtgtccgg agtcaacaaa taacttctga aaacaccgtt
    58921 tggtgaaagc gggaataact gctgagttct cactctgaaa ccaagtaccg ggtcccctat
    58981 cgacatgaag tttctgtact ccgcataacc cgaaataaat ggctcgttat cctttccaaa
    59041 taaacctctg accgtcagtc cggcaaggta ctcacttccc atagtaggcc tgtaaagatc
    59101 gtctcgggta gacagctcat agctcaatcc agtgaagata ccatcaaaat tttctgagag
    59161 actacccgtg tcgacttccg tagcagtttt agtttccgtt gagacctggc tttcagacat
    59221 tatgtactcg taaccggcgg taaaaccgac tttctgccca ggggaaacat tataaatggg
    59281 agtcaaagag aacaagaact ccgtttcctg ggaggatttc gtgactatag tgtcagtcga
    59341 atcatttgtt gtcgtgaatc ctgagaattt aaacttgaat gccgtatcga gatcaagttt
    59401 tgttcctcct acctttggga tagaatagcc tatagttgca tcaaaggctg gccttcttgt
    59461 cgtagtctct gtagctccgt cagtcacgat aacttcctta ttgttcggca tgggaagcgt
    59521 aacactgagt gtcgttgaga tgttctgccc gtagccccaa acattgagaa gtccgagatc
    59581 gagatacaga ttcaacccat tctgccagcc tcctcctccg ccgatcttat tattcttatc
    59641 agtctcctga agcgtaatgc ttacgtcgag cgcatcctca ccaacaggaa caggggcaat
    59701 atcaactttt gaaaagtagc cggtattgtt caagaaagcg tatgtatttc taagctcatc
    59761 aagcgacaat acattgcctt tctgaatgac tatcttgtcg actatcaaat aatcctgtgt
    59821 cttcttagga ccgtcgaatt caacggttat gtctcttact aaaggctccg taatcttgat
    59881 actgaagaag ccgatcttct catcaataac tggttctatc ctgataaaag gataccctct
    59941 ctcagtatat aagtcctgaa ctgcttgaat agatctcatc gctacctcgt tgtctactct
    60001 ttgaccttca gtgagtcccg tctctcttag gatttcacct ttctgtagat tgacgttccc
    60061 ctctatgaag acgcttctga gatcgaactc ttcttttaca tattcaaccg gcgcaaaagc
    60121 aatgctcatt gatagcgctc tgctttcttt aactccctcg ggaagtgtta cctgtcttgc
    60181 gtttctcaac gcgttattca acgtaagaat cattgcctga taagtctcgt catcaaatcc
    60241 ccattgaccg ctggaattcg cataatatgt gctgtagact gtctgaacca tcatatttat
    60301 gtcgtctagc ttgggcacgt acttcttctt agaatttatc catctaacca aaaatgatgt
    60361 gtcataatac ttcttcaggc aatcaatctg caatttgctc gacagaaatg ccttggactc
    60421 atcagatagg ggtgcttcaa tcgatacttc ccacagaaag gatactttga ctgtaaatgt
    60481 gacttttact ctatctttta tgttgccaga aaacacgtct tttctgcttt tatcggtttc
    60541 ccactctacc tcaacaaaag gttcaatgta gccgttttct tcgagtttgc tcatcatagc
    60601 ttcctgagtg aggggaatat catagatcag tttcgaggga tccgccggtt taccgctctc
    60661 tataactgaa accgattcaa gagcgtcagg ttcaacgatt tttggaccgt caaaaacaac
    60721 ttcaacatcg acaacagggt attccacaac ctgaatcttc aaaagaccgg aagattcatc
    60781 gaaactatag taaacatctg aaaagtaacc gctctttcta actctttcca gtgcctcact
    60841 gagttctgaa tcgctaatct cttttccccg ctgaatatca agtatctcaa gcacctctcc
    60901 atccgagatc actctgttgt caagaacggt aattctcgaa ggaacaataa ctgcaaaaac
    60961 agatcctgag accaggacca acacaagaat caagaacaat cgtctaacgg acatctgaca
    61021 aacctcccag ggatatttct gcctcttgaa gaatggtcaa gaccatcttc attttgactg
    61081 gtgaagtgaa gcagtcgttc ccgaaagctt ctcgaaactg cgacgacgaa aatgcatacc
    61141 cctcgtcatc cagtaatctc tctatagacc agtctacacg gtcgctcaat gtaataacag
    61201 caaatagagt caaagggaca tctcctacag gctgacaatc tatactcgag agcaagaact
    61261 tcgcactcac ttcagttcca gatccttcga ccgaacgaat cctaaattcc cctccggtgg
    61321 actccgcagc aaacttaagc ataggaagcc ccaatccgaa tttcactttc tttctctttt
    61381 cagtatagaa gggatctaaa acggattcaa gtgattcact cgaaattcct tttcccctgt
    61441 caacaaccga aaagtaaaaa ctctcttctc tcttctcgac gattctcaat gtagcttcct
    61501 ccgttccaga gtcgactgcg ttctgagcga tatcaataat gtggtcacat atggttctca
    61561 atcccatatt attctcagcg accttccatt tgaatagaga gcgcttctaa actcctcgaa
    61621 gcttcttgcc tttgactcaa tcgaaacggt gggggtgatc atacttaaac tgtgtgcatc
    61681 acttgaatga atgatgttct ccacaggaag tttcttttca agggttctct tgaacgaaat
    61741 ctccatcggt acttccctgc aggtctcagg tatgaaacct agctgagtca aaagtcccat
    61801 ctttctttca acgtgagcca ttacagagat tccgccatac tcctctacgg ttgacagagt
    61861 ttgcgaaatg ctcaagagag ctggttgacc tagccagatc tcttcgaatc cagtgaaggt
    61921 atcatcttca tcaacgatta actgatagcc tgagatctct ggatcgaccg ataatcgcga
    61981 aaacttttcc tctttcagcc actcagagta atcctcagct atctcaacct cgggaaaata
    62041 agcgagtata tgaacatctt ccaaagtgtg tacttctatg ccaggtatga caactacacc
    62101 aaacctgttt agtactttac tgaaaaccct gacattcctc gcactgttgt gatcggttat
    62161 tgcaacccaa tctattccct ttgaaacgca tgttaaagct attgcattgg gagtcattgt
    62221 gatatctgcg cagggggaaa gacaactgtg aatatggaag tcacaaagaa acgacaaagc
    62281 ccgctagcct ctcagcccac tcttgataag cttgagcgca gtatcgaagg tagtataatc
    62341 gctttcaaga aggacaatac cagtttcttc tgcctttctt acagttgatt cctcgaagtg
    62401 aagaccgttg cacaacacga tcgctcgcac tccaactatg gaggccactg caagtatatt
    62461 tacatgagac tgaacggtca accagattga atttgagggg gcattgccca tcacatcgct
    62521 aagtaaatca ccaatatatg catcggaaat ttcaatgtct agaggcaggc tagtaattac
    62581 tctcattcca catttctcaa tcagatcgtt cagtttcatt ttatttcctc ccattccgat
    62641 agaaatgcat ttccaagacg actccctttc cggcttcgga tacaacaacc attttgtcgg
    62701 aataccttct catgtttgaa agacccattc ccgccccaaa tcccagttcc ctgacatggt
    62761 ctgaagcggt ggaaaaccct tccttcattg cttgttccac gttctctatg cctcttccat
    62821 aatcctccac tctcactttg gttacgtttt catcgtacca ggcaaaaatg taaccttcac
    62881 tcccactgtg aataactacg ttgacttcag cttcgtaaca ggcaatagaa actcttctta
    62941 ttagcgtgtc atcatccgtc agagttttaa gaaaactctt gagttttgca gatccaactc
    63001 ccgcagtatc gatatctgtg taatcaatat ggtaaacaaa atcggcactg gtcttctcaa
    63061 cataatctcc cgtgattagg gacttgtcaa accagtcaac gctctgttcc aagacctctc
    63121 tccgtcgttt gtcatgaaca taaatcaatc taaatttgtc aagaatagca ccaattatat
    63181 ccttcttgga gataatccct acaagtcttc cgtcggaatc aacaaccggg aatctgccat
    63241 accgatatct gttgaacatt tctattaccg actcaagttt catctccgga gagaaagtga
    63301 ctatattccg tgtcatgtgt ttttcgatcg gatcagtgat gtagttaccc tccagagcaa
    63361 caataatgtc ttctatactt actatccctt cgagttgatt gttaccgtcg gtaacgggaa
    63421 taccggagat cttgcaaatc ctcatcagct ctttcgcctg ccacattgtt ctatccttgg
    63481 tcagtgttac aacactcttt atcattatgt ccttagccgt gatatctgcg aataaccccc
    63541 ttagtttatc tagaaggtcg tcgacatttc tttcagtcaa agtgtttccc ttcctcaaga
    63601 gaatctccca agttctcatt gaagagaatt ccgcaggctc tatacattga cattctcgcg
    63661 tgaactagcg gcatatccag ctcttttgcc aactctatag ttgtctccgg cacatttctc
    63721 tttctaatta tgacaacggc tgttgcaccg actacggaag ctgtcctgac acactgtgga
    63781 gagttgagtc cagttaccaa aatcattccg ggacctccga aagccagcac gtcgctcatc
    63841 aaatcggcag cacctaccga ctccacttct gggtctccat caccgcagta cagtatttca
    63901 catccgagaa tctcgatgat tcgtgataga gtcattatca ctgagcctcc ctgttgagaa
    63961 gatctcctgt gtaactatgc cgaatgaaag catcaaaagt tactggacag ccagttttaa
    64021 agatttcggc tatggcgatg gcatactcct gaatttctgc ttgagcatgt gaatgggccc
    64081 tttgattaag aaaattcata attgatctgg cgtttactgt ccagtaagcc tcagtgtaca
    64141 ttgaggtcgg cagaactatt cttgccatct ctttggctac tcccatttca attagccttt
    64201 tatatgctgc aaatgcgttt tcataggctt cactgataat cgatgttgct ctttcgttca
    64261 gttccttgtc tttgcagaca atcgaccctt gtctgttgtc tttgtcaggt attctgatga
    64321 cgcccggtat ataccactct tcggaaaact cagtatatct tccactgcgt tcattaatag
    64381 acgcaattct gtgtcttacc agttgcctca ttacaaatat gggaagcttc aagtgaaact
    64441 tgaaaatgat gtgttcaaag ggagattggt gaccgttttt catcagataa tctataagcc
    64501 gatcatctct ctccttattt gtcagaccct tgccatagct tgtccttgca gcttgaactg
    64561 cagaatagtc atcacccatc ttatctacca gctctacaaa tcctctatcc agaacatcaa
    64621 tcttcattgt ttcctccgtc ttctttcatt gccttccttt cttgtgaagt ccttctcttt
    64681 ttactaaagt attctaacag aaagagccca acaacaacta tcgcggcaag cggaactatt
    64741 gtaacaagcg caagctcgaa aatcgctctt cttcttagct ccttcaatgc cgaactcact
    64801 tcatcgtcaa aactctcgta tccgctcagc tgcagataga acctagcgag atcatagtag
    64861 tctcgatctt tcatcagata gtatgccatt ccaagattcc tgactgccat tgagttctcc
    64921 ggccaaaatc tcaaagaatc ttgatagttc tttaccgcct cttcaaaatc accgtttcca
    64981 aggtaataat ttccaacgac cattgaggta tctgaggcga acctgggtgg gattcggtgc
    65041 aagtctatgg aatttaggat ttccagagct tcagaggatt cacccatttt catgtaagcc
    65101 atagccgcat aatacatcga aacaggttca tctgtaccca atcgagaata atcttctagc
    65161 acaagctctt cgagccccga aacgaactca ctttcaatta gatcatctcc gagcaagtct
    65221 tgaagacctt cggagaatat tctttctctt atctccataa gcctatcttt atcaatcgaa
    65281 ctgccacttg agtatacttc cagaagagct ttatagggga aatactgatt aggtaataac
    65341 tcaatcaact catacgcagt ttttacaatc tcttctgatg atcttggatc tctcgtatcc
    65401 acccagtcat agaagaaagc cctcaaagaa atcgccttta tcagattatc gctgttttga
    65461 gtagaataga gcatattcaa agccgacact gtagatacgg aaaccagttc attgacaatc
    65521 ttaatccctt cctcaatctc agatggaagg gctttatcct tcagttcctc gtagaggttt
    65581 gagaaccaga ccttgtagtt gggctgaatt gtctccctgt atttcagacc aaccgcgatt
    65641 gctagaatgg ctttcgtttc gacactttga gtctccagcg actgtagttc aatcagatac
    65701 tctttggaca gaaggagctc ggcattgtca agcgcaaaaa tcaaagatac agacactaga
    65761 aagatcgcga cggcaaatgc tgacttcatg gaatctcact ccaaaattcg ccaagagtga
    65821 ttgcgagaaa tggtttagct gattccataa cgtcgtcaat tactagataa agacttggtt
    65881 tctcttcaat cgttacgcaa accccagtac cagaaaaacg actggtctcc ctgaccgaga
    65941 aatactccca gtcaaatctc ttgacactct cgtagtgacc aggcatcagt gaggggttca
    66001 cagccttcga agccttttct atatggagct ccctccctct gtcccaatcg taaagtctgt
    66061 aagtaacatc gctgttctgc tggatctcta ttaactccga acccggacct atcgcatgaa
    66121 caattcctgc aggcaatgca agaagatctc cacgcttcag aaggatgtga ttaagatcgt
    66181 cttcccttac gctgtcaacg ggtttggttt tattcttcat tccagcaaga acctgccctt
    66241 cgtttaagaa ataccagcat tcactctttc cccagggctc tccttcatag agacgggcaa
    66301 acttgtcatc aggatgaact tgaactgaga gccattcgct ggcgcagatg tgtttcacca
    66361 tgattgggaa tctaggcaaa gatcttcccg ataacttgtc tataacttcg tttggtgata
    66421 gtcttgttga tcccgattca agccatgttc tcattccttc aatatctgaa agaagccata
    66481 cttctccaat tggctcctcc gaatcaactc cgtatattct atttagcttc cggtcacccc
    66541 agggcctcgg tgaaaagaca gggcaacttc taagtatcat cagatgatca cctccaggaa
    66601 acgttgaatt ctttcctgag caacaaccac tttttttcta ttcttctctc tcctgttttt
    66661 cattctgatt tccggcagta aaccaaaatt cgcatatact ggcttcaaag gactccgacc
    66721 ggataccgta atgtggttta tcaatcctcc aatcatagtt cccactggag gaatcacagt
    66781 ctgtttacct ctagcatatc gatcagcatt gatcgcggcg agccttccag agactatcgc
    66841 ttcaacataa ccttcgagac cacttatttg cccagcaaag aacaatccct tgtgattcct
    66901 ggattgcaaa tcgggattca gaagccttgg cgagtccaaa aaggtgtttc ggtgcattac
    66961 tccgtaccta attatgtcaa catttctcaa agcaggaatt agggagagaa ttctcttctg
    67021 ttcccgccat ttgagtcttg tctgaaagcc aactattccc aacagcgaac ccgacaagtt
    67081 ctcctttcta agctgtacaa ctgcgtacgg ctccttcccg gtaatcgggt caatcagtcc
    67141 aacaggcttc atcggtccga agcgaagggc atcaattcca ctacgagcta tctcttcaaa
    67201 aggctggcac cgttcaaata gaaagcgatc cgagaagttc tcgacctcta acacttctgc
    67261 atgcaccagt tcattccaga aaagctcgta ctcctccctg gaaagcgagc agttcacgta
    67321 gtcgccatcg tcggagtatc tgtcagctat aaatgctctt tcaagatcaa ctgaatctgc
    67381 agctactatt ggtgcaactg catcaaagaa gaacagggca cctccgaaga gttcaccgag
    67441 aaaggtttca aaacagttag aagtcaacgg accggtacac acaacattca ccgctccatc
    67501 gaaatcaatt gagcacacct cttctcttct gatatttatc agtggatgcg tctccaactt
    67561 cattgaaacc tcttcagaaa acgccttacg gtcaacggca agggctttac cggctggcac
    67621 tctgttatga taggcgattt caaggagcct ggatcctagt ctcaaaccct cttctttcag
    67681 caaaccggaa gcgttatcca gtgactcaga cttaaaggaa ttgctgcaaa cgagttctgc
    67741 aaagtcacca ctttgatgga catcggtttt cacatttggt ctcatctcaa aaaggttaac
    67801 ttcctgccca aaatcggcga gactgagtgc ggcttcagat ccggcaagtc ctccaccaat
    67861 aatgttgatg atcaattcag atttctcccc aacttgtgag ttctgtaata tctaaacagt
    67921 gcttcctgat agtatccacc gtaaggaaaa aaagagttca ttccaaactg catgatcttc
    67981 tttgtcgagc cttcaatacc atatatgtcc ttcatgactc tgctgatcca aatatcaacg
    68041 gggaatgcgt taagttcgcc gtaagcaaat aagacaacgc aacttcccac tttgtagcct
    68101 ataccatcat gccttgtaag ctcttccagc ttttcttcaa cagtcagtgg tatgagctgc
    68161 gaaaaataag actcctgatc aattcttgag aaaagtttca ttagccatgg cactctgaat
    68221 ccaattttga gattagtcag caattcttcc gaaatgagct ttgcttgacc tagagtaggg
    68281 aacttgtaaa acgtctctcc acagaagtcg accctgttct cggggaacgc ggcagacagg
    68341 ctatcactca tccagcgaat catcgggatg ctatttctag ttgaaagaat gtattctact
    68401 accatttcga atgggtcctg tctgagtatg cgaagaccat tggcctcaga tattgccttc
    68461 tccgaaatga atctagcttc ctcatcgaat ttactaagcc ttctcctgag agtcgaatga
    68521 atttcgtcaa gatcgtcttc aagaccaaga tagtgtatta tacccgtaga aatctccctt
    68581 ccaagaagag aattggaaga tgactttacc gtgatctcgc ccagactttt ctttatgaag
    68641 aggacagtat ctcggactac acccttccac cattccccat ctttaaccca tctaaatgtt
    68701 tggccgcaat ccagtgttga atccagatct atgggctttt caggtctgaa agagaattca
    68761 atcatagttc tacctcataa gactctacaa tcgttttcaa gagcctgtaa gaattgtctt
    68821 caagaaactc tccgactact attaagtagc acttttcttt gagattatcc atatgcaaag
    68881 cagtaatctg aaccccttca accactgtag agcttcttga accggtcgct cttgatagtg
    68941 caagaatcaa tgaaggaatt tcaaagtcaa tatccccatc ataagggatc ccattttcgt
    69001 ccaccaaacg aaaggtcatt ccacactctt cgcccagttc ttttattctt ttagctaagg
    69061 caatcattga tccttctcct taagtatcct gcaattgtag gtacctgtct tcagccatct
    69121 cttgttcttc agctcggcgt cttctaaatc gtcaaataga gttgctggct ttatgatttc
    69181 aatcagtttt ttcagctcgg aaatatcgtt tacaggaatt tcgcttcgaa gaacaacctc
    69241 tcctctgaat ctccaaaggt tgaccaaatc gccaaagtct cgctcgagcc tctctctgtc
    69301 cctagtgtct cttatctctc tctctttctt cttctcgctc agactggctt tacaaccgca
    69361 gtagtcttgt ctgtaaatgc ccctgatcaa ggtattcagt ttccctctgt ccactctgaa
    69421 attcccagag atgaactcaa tacttagatc attcgcaatc ttatccccga taagggttat
    69481 gagcttaaca gacttcctgg ggctcgctaa aagagtagtg ctaaaactct cagcgccaat
    69541 tgacttggcc atttccgcag ttttcttaag cctcagctcg atgcacttag agcacctaat
    69601 tccaccttcc ttctcatcaa cataagaatc gagaattttc gaaaagtctt ctgggtagta
    69661 acttggctcg ggacacacaa ctgacaaact ctcacagacc tctttaaaag ctttatatct
    69721 tctgaaaaac tcctcttcgg gaaatatgtt cggattgtaa aagaaaagct ttgagtctct
    69781 gagctcttcc atcttaaaaa ctgtgcttac cagatccggc gcacaacaga catgtagcac
    69841 attcaaaact ctatcagctc cttcaggttc gcgacggttt tttccccaat cccgcttact
    69901 ctctccaaat cctcatatga acgaaagggg ccgaatcgag ctcggaattc gactattctt
    69961 ttggcaataa ccggtcctac gcctggaagc ccacaaagag cctcctcatc agcagtattg
    70021 acgttcacct tcaaggaacc tgattgcttc tcgcttccag caagaggaac aatgctagcg
    70081 agaatctctg agagaattga ggggccgata cccggtactt tcagaaggtc gtccgaggtc
    70141 ctgaacgatc cgtttttttc tctaaactcg actattgcct ttgctttcgc ctccccaata
    70201 cccggaatat cttgcagtga caatacgtct gccttgttta tgtcgattag ggtgtttaca
    70261 tctgcaggag tggttgcgga acccgcaaca gtcaccattt tcctgaaact gcttaacgtc
    70321 ttctctccga tacccctaac ctggagaata tcttcgacag agccgaactg acccaccgag
    70381 gttctgtaat cgataatagc tcttgctttt gccggaccaa ttcctgggag tagctcaagc
    70441 tccctctctg tacacgaatt caagtcgatc ggaaaactaa cagactccgt atgatcgaaa
    70501 ttctgttcgg tgctttccgt cccggtgtac ccaatgactg caacaaaacc catcaagaca
    70561 agaattacaa ccgaaccaat tatctgaagc tctttgacag ttaacttcct catttaagtt
    70621 gcctcggaaa tctgtctgtg atattccatc ccgttatcct ctgtaaagac tggacatcaa
    70681 cttctgagga ataattccga gccgcattgg catccggaaa tactcccagt atagctgcgt
    70741 agaaggttct tccatcagtt gttattgatt tgtatacaaa ggcaggataa ccggcacttc
    70801 tgagatccat gacctgaggc gctatgccgt cagatactgt atgagaaaca agctgaattc
    70861 cataagcggt ttttgagtca taaagaccgg taagtgggtt ttcaccaaga aacataacac
    70921 caaattgatc atccacacgg gtaataaaat acttacctaa acccttttct acacaaagtt
    70981 tgaaggccgt tccttcttct acaaccacat aaccgaactc agagccttca gccaagaaat
    71041 cgaaggaatt tgttattaac cttcggtaat cgaaagtctc aaggaagaag ttttgagtct
    71101 cgggaactga gatcgcttcc ctaattatct gtgtacctgt cggaatttca acggaatttt
    71161 ctgatacttc tggttgatcg gttgaagggg taaaaaccga gcttcgaatc tcttctatta
    71221 cgagcttcgc tctacggttt tcctctcgaa gcagaattgc atagcctcct aagataaaaa
    71281 caatggcaga tagtccgaga acaactacaa tcaatgtctt caagacatag tcatgtgatt
    71341 cttcgctcat gcaaaactct ccaatcctct ccacgttttc tctctaatga acctttcaaa
    71401 tgaagaatac ccgacaaaat aagttccgtc tagactaccg tcgggcgtac tgatttttag
    71461 accacccttt tgttcaatca gatcgaccaa ggacgataaa tcaattctcg agggccagtt
    71521 tctgttccac agctgcctca ctaaaccgaa aaaaacctcc gagcttttaa cgtcattgtt
    71581 actgaattcc ctggcgagcc gcagttctac cattgcgccg agaatcaccg ctgccctatt
    71641 tccggttctg agtctcgaag cagtttcaat gagtcctcca gtttccgctc caacaaatgg
    71701 tttcttgagc tctattccct tcaaataggc ttttacagaa gagaaaacaa tatcatccca
    71761 ttgctcttca gtaatctctt ttccagaagc cacaaggttt gaaatgtatt tgaacaatct
    71821 catatcgaaa gaaagggcaa ctagtaaagg aaagagtaga tcacttctcg atgagtcaag
    71881 atattcttta tagcttatcg aagaatcaat aaacactctg tcaggaatgc catttgcgca
    71941 gagaagatcc ttgacaaact cgaaattcaa cttgaatccg ttaatagcgg gcataatctg
    72001 accaacggtg gtcgttggaa aaaggaagag tttttccact tctgtatggc ccgcacacgc
    72061 gtatcctacc agatctataa ttgaacctcc accaattgca gtaacggctt tacatcttga
    72121 cactctaacc agctgataaa tctcgaacac cctgtcgata gacttaatat gctctccgcc
    72181 aggcattaag tattcaggct tcaaatttcc cgcaaatgac ttcttgaacc ctgagtctac
    72241 tactctaatt gactcaggga gcttctcaat catattctta ccaaagatga gctgacatct
    72301 ttcggtcgat tcgctcgtaa atgtaattct cttcattcca ctccttcttc tgcaagaccc
    72361 ttaagaagac gaaccgcatt caagacatcg tcatagtcga ccatttcgtg aggagagtgc
    72421 acatatctac atggaatcga gacggtcccc gatgctattc ctctcccagt tgtctgataa
    72481 cctcttgcat cagtacctcc aaaaatcagt acctcatact gggttggaat ttccttcttt
    72541 tcagcaacct ctttcagtct tgagacaacc cttgcagaac ttacgcttcc tcgatccttg
    72601 atttttatcg ttggacctcc tccaagtttg aagcccattc tcttaaagga tttgggagtg
    72661 tcagggccag ctgtaacatc tatcgcaaca gccatatcgg gcataatatc gaatgcagcg
    72721 acactggcac caacaattcc gacttcctcc tgtacggcaa agacaaaata tacgtcatct
    72781 tttggtttat cgagctcttt caatacctga atcattattg cgcatgcaat tctatcgtcc
    72841 atcgatttgg agacaattct ctttccaaga tcgacaaagg ttgaatcata ggttccgaaa
    72901 gtaccaatcg gtgccttatt ctctgcgtcc ttcttgtcaa ttgcaccaat atctacaaaa
    72961 acatgatcgt agtccaggtt ctttatagtc tctcctagtt cctttgccgt ctcaccttca
    73021 acatccacta ctcccgaagc tcctgtctca aagatcaatc tcgaaccgag aagcatgtac
    73081 ggagacagtc ctccaactga atcgactctc aagaacccgc ttgaatcaat gtgagtgacc
    73141 acaaccccaa tctcatccat gtgcccgtca aacagaagct tttttccact acttcctctt
    73201 ttcactgcaa taagatttcc taaaggatct atcttcatct cgtcaacata aggctttatc
    73261 tcttcgacaa tcacttctct gatggcagac tctctgccac ttggagaact aacagtgaca
    73321 agcttttcga taagtttctt catctagaca gcaccttccc ttccttaacc agtatagttg
    73381 caagttctac tactttaaga aaatcgttga catttattac cgaagttggt gaatgaatgt
    73441 atcttgaagg cgtggaaatg actccagatg gcacaccggc cattactttc gcaagccttg
    73501 ctgcgtcagt tcctcccgct attctgctct tatattggaa ctctatcgaa ttgctttttg
    73561 ctgtttcgac aatcgtgtca aggatcttct tgtcaagaac caaaccgcta tgagcaaaag
    73621 tcagtacggg accttttccg agacttgtgg cccatctgta ctcgggcagc tctggattgt
    73681 caccggctgt cgtattctca aagactagcg caacatcggg cttgatttgc ttcgcggcaa
    73741 caccgctacc tcttagaccc acctcttctt gaacgaccca tgcgaaataa acatcgaaat
    73801 caaccgtttt ttcgtcaagg ctttcaagaa cccttatcaa aacttcacaa ccggttctgt
    73861 cgtcgaaagc ctttgcgact gcatagtctc ctatctcttc gtaccgagta tcgaaagaaa
    73921 ctggatcccc tattctatgt ttttttgaag cctcttcctt ctttgaatag cctaagtaga
    73981 ttttcagctt gttcatgcta ggagttttca aaacagaaga agggtcttgg gtatgaattg
    74041 cctcgaatcc aataacacca ggtgtcaaat cttcaccaac caaaaccttt tttccaatca
    74101 gcactcgagg atcgactcct ccaatgttag tgaaagagag cgtaccatct tcattaatct
    74161 ttttgaccat aaggccaact tcatctgtgt gggcaaggag cataagtctc tttgaacttt
    74221 ttccatttcc tttcctgata gcaattagat ttccaaccgt atctgtccag atagagtcca
    74281 cctttgattt gacaacttcc ataatgtatg aagacactct ttcctcaaaa ccggagattc
    74341 ccgggagttc gcacagttct ttcaaccgtt tgagcttcat atttcctcat ctccttttag
    74401 cgagacgaag aaaccggcaa gcaatcttgc agtacctgca acgtcactaa tctgaacaac
    74461 ctcaactgga gtatgcatat gcatttcagg aagtgaaagc agtagagtcg gaatgccgat
    74521 accggctatc tgcacattgt cagcatccgt tccagttcta ccagaaccaa actcatactg
    74581 aacctttatt tcgttgtcct tcgcgtagtt gttcagtaac tcaaaatact tcttgtggat
    74641 attcggacct ccaaccgtca aggccggccc cttgccaagc tctatatcac tctctttatc
    74701 atgatgagtt acatccatag caaccccaag atctaattcc agagcttctg cagctccctt
    74761 tgctccaata gcacctacct cttccctcgt agtaaaaacg aagtaaaccg tgggcgttaa
    74821 actatacttt gaaagttctc tagcagtctc tatacttata gcggcacaag ctctatcatc
    74881 caacgctttg cctgaaacct tgccgttcat ctcaaatgca gaaaaatcta ctaccgcaag
    74941 atctccgaca tcaatcttct caaagtctgg attaatcgaa gcatcgataa acaactcgtc
    75001 aaaagaaggg acctctcctc ttgtctcttt cttctgaaga tgtggagcca gcatgccaat
    75061 caccccggcc ctttcttctc cgtctctgca tttaatctta accttctgag atataagaac
    75121 tttcggatct attcccccca gttcttcaaa tctcgcgaac cctctttcgt cgatctttga
    75181 aatgaccaaa gacacctcat cgacatgagt gaatactcca atccgcttct tgtcaccttt
    75241 cttaactgcg attagatttc cgatctcgtc agtgtagatc tcatctacaa agggatctat
    75301 cgcctcttta attactgaat gcacttcact ttcgtagccg acaggtccga acgcattgga
    75361 gagatctact aacaatctgg ctagattcaa ttcgcctccc cctttacttc aaactttaca
    75421 ttgtctgcct cttccactat gattcttccc gttctaacat gggcaacggc tggaccatca
    75481 aaatgatcat attcttgagt gatgaagttg cctatttgga tgagcgacgg ttgtaaatcg
    75541 atagccgcaa ttactccagt ctcgccctgg gaataaccgg ctctcagaac tcctttcgca
    75601 cttccgaata tcactacgct ccctccagct attatttcgg cgcctggatg aacgttgcca
    75661 ataactacta catcaaaatt atgcacaacg atttgaccag atctcaaatt tcgcctaata
    75721 atctgagctc cccgaacttc tgtgatcttc tctcttacga gatcatactt ctgaccgact
    75781 ttgacgtcct tttgctccat ggtgccaacc aggatgtctt taacatgaac gccgagatca
    75841 ctgagcaatg agacgatttt tactatatcg tcgggcttac ttgtatcttg agtaagcatc
    75901 aaagagatct catctccttc agaaaaaaaa tcctttgcat cgacgaactt cttcatgatg
    75961 tcttgcctga gttcgtcaac gcttctgtaa gaatcaatta gaagtatcag gcccttctta
    76021 gtcattctga aatcaatcgg cacagtgatc acccctttct tcaagattct atcacgaaaa
    76081 aactctgtcc aaggcgcact ggaattgaag aagtgggctg gatgaactgt cagaaatgca
    76141 catcggcctc ttgaccgccg acggtttata gtgtaatata aaatacgtgc ttcggccctc
    76201 atcgtctagt ggcctaggac actggccttt cacgccggcg accggggttc aaatccccgt
    76261 gagggcgcca gtggtggaag cgtagctcag aggaagagcg tctgccttac aagcagaagg
    76321 ccacaggttc gatccctgtc gcttccacca gattgatggc gaggtagctc agcaggtaga
    76381 gcagcggact gaaaatccgt gtgtcgacgg ttcgattccg tcccttgcca ccagttggtt
    76441 ttttcttacg aatatttctt tgtttgatat gtcctccggg gcatattttt ttgaaggtgt
    76501 ttactgtaaa gtagctgcta taatgtaatt gtttccgggg gtgagaatga tagagaaggt
    76561 ccagaggcac aaatacatta tgaatctact tgaaggttct tctgtccctg ttaagggaca
    76621 gtttctcgcc gacgaaactg gtgtcagcag acagatgatc gtgaaggaca tagacgaact
    76681 gagaaaaaat gggtcgagta ttgtcgcgac aacaagggga taccttctcg gcaaaagaag
    76741 gaccttcaga atggttgtat cggtgaaaca tgatgaagaa gaaatttatg acgaactatg
    76801 ggaaatcatt aaggctggcg gccatgtagt tgatgttagc gtaatccatg cagtttacgg
    76861 agagctaaag ggcaaactag acctttcgac gatagacgac cttaacaggt tcatagctgc
    76921 tctaaaagca tcaaatgcag ttcctcttct cagtctttcc aaagatggag ttcatctcca
    76981 tacaatcgag gctgactctc atgagtcttt agaaagaatt agaagcattc taaaaagaaa
    77041 gggttacttg cttacctgaa ggaggctctg atgaacattg atgaagtgat ccaaaagtat
    77101 ataactaagg gcaggagaac gcgcatgctt ctactgactt cactggagtg gatgttggtg
    77161 gccggaggag ttatgattac ctctttgaca cttccttcga tcgtggaaga tcttgccggt
    77221 actacaggca gtcagaatat gatggcaagt tcggtattcg tgggaatgct gctcggtgcg
    77281 ttgttttccg gaatgatctc cgataggttc ggtaggaagt ggacaaatct catcttgttg
    77341 ctaattgcag gtctttcaac cggatttacc ggcgcatcgg tttcaatgag agctttttcc
    77401 attggaagat tcgtagccgg aatcggctat ggtggtctgc ttcctgtagt caacgcatat
    77461 cttactgaat tctcatctat aaagatccgt ggtttgtatc tcgctcttct tgaatcaagc
    77521 tgggccattg gaagtataat cactggtgga tttaccatgt tgactttaaa cagccttgga
    77581 tggcggtggt ccttctactt tcttgcatta ttcagtgtac cactcttgct aatttccttc
    77641 tttcttcctg aaagccccaa gtatcagttc atgaaaaagg gcaaagtcgc actagagaaa
    77701 atactgggaa aagagattga tgaagagata gagatgcata aacagtctaa gcaaccaatt
    77761 ctcggattgt tcaaaggtga tctggctaga aggacaataa tgatctggat cagctggttt
    77821 tccgtaagtt ttgtctatta tggaatctac acgtgggcgc caaagatctt catgtccaag
    77881 ggactgacgc ccgtttcttc cctgtggtat acctttttca tgttgatcat gcaactgcct
    77941 ggctatctga ccgcagtgat cctcattgaa agaattggga gaaaaccatc actaacgttc
    78001 ttttttggag gaatggcggt ttcgtcaatc gtcatggcac tggtgaatag ttcagctctt
    78061 cttctcgttg cgagcgtttt gatttcgctt tttgtgcttg gagcatgggg aatggtatac
    78121 gcatatacac ccgaattata tccaactgaa atgcgtgcgc ttggaaacgg tgcatctgga
    78181 gtgatggcaa gagcagctgg aattgtcgct cccttcttca cgaatttggt tatgggaggt
    78241 acaggcagcg tgtttgtagt gatgatattc atggctgccc tgagtttgtt tgctgcgata
    78301 atcgtatcca aaatgggcgt tgaaacaaaa caggcgatca tcgagtgatg ggcatggagt
    78361 taatcgacag gatcatcact gttgggagtg aaccaatagt tgtcttcccg agaagaagaa
    78421 cctgtctcaa ataatcagga ttactgctat ttatgaatcc tgagcactcg cgacagttct
    78481 ctctactcca aagcaacctg tccatctttc ggcaccggaa acttgtaatg aagtaaataa
    78541 ggatggacaa gattcatcca attaatgaaa tctgtatcca ctctagttat tgccgagttg
    78601 aatctatagc atcaataatt gatttcatca aaccactgag attgatcgtg ttgacttgtc
    78661 acttcgttca ctgcttttat tcttttcatt atcgcggttt atgatctgct ggaggggttc
    78721 caaacgaaaa aacgactcga caaatgtcca agctgcagta cagacttgaa gataactgag
    78781 tatgcttgtc agaaatgcaa ggcaaagata atcggatcat tttcgcgggg agaactttca
    78841 tctttgcaag aagagcttct tgggtttttg aagaccttca tcaagaatag aaggaatctc
    78901 taagagtttc gaaaagagtt aaatattccg tattctacag ctaaaagtaa actggaagaa
    78961 ctggtaaccg gtctcggatt tgaagttgcc aaccgtcaag ttcgcgaagc atccttaata
    79021 atcaagaagc tcgaagagtt gaaataacac tgaagaggtt gctcagcttc tgaacaacat
    79081 gaaaaaggga agataatagc agatggattc atgccaacag aatttgctct taagatactt
    79141 cacgtagtac cggcagatta taattcttct caaatttcag accataagag agttagaaga
    79201 tcattatgcg aaaaatggta tattattgtt gctcgacaac ttctgtaaag gggtgtcgcg
    79261 aatgaaaata gttaggacgc tctttctgat tctgcttatt gtcattgcat ttgcagttgt
    79321 caacggaatt ttcagtgtga cagtggcgag ggtttttttg aacaggattg aaagggaagg
    79381 taggtacttg aaggtcggtg aggaagagct ttttctgaag gttctcggcg aaggaaaacc
    79441 gatgcttatg attcacggat ttcccggatc gcatcttgat ttttcagaac tggccaagct
    79501 tctctcttca aatagaaaag tctacctagt agacctccct ggatcaggat tatctgaagc
    79561 gtcaagcaaa ggagactact caagaaaagg atacgcggag cttcttgtag atttgatgaa
    79621 tcttctgaac attgaaaaag cagatatagt agggcactcc ctcggaggag agatcgcgct
    79681 caacatcggc tactactatt cagaaagagt agagaatctt attcttatag attcttacgc
    79741 actcaccgaa cagaaactcg tgcctgattt catttctagt agcaaagtgt tgacatggat
    79801 tgcaatgcga ttctattatc agacctatcc tgttcagagg tatctctata ctaaaaggct
    79861 gggagacaaa agttactttg tccatgagga gttcggaaag tattttgctc tcgtagatcg
    79921 tatgtctata cagttcatta gtgagttcat taaggacagc gatggcggtt ctctttcggg
    79981 aatgcttgga gaaatcgatc tgaatgtatt gataatctgg ggagaaaggg atgaaatact
    80041 tcctttagat tatgcaaaaa agatttctga agagatttct ggaagtactc tcaagataat
    80101 tgagggaaga ggacacgctc ccttcaccga caaacccgaa agggtggctg aagaaattct
    80161 ctctttcctc gaataaaaag gaggacacaa ctttgataca ctctagaccg caagtcgtgg
    80221 tcagtagatg tctcggattc gatacctgca gatacaacgg ccagatgatt ccaagcaaat
    80281 tcgtcaaaag gctgaagaaa tacgtccagt actttaccgt gtgtcccgaa ttcgatatcg
    80341 gacttggtgc accgcgagcc cctgtaagaa tggtaagaaa gtccgatgga ataaggttaa
    80401 ttcaacccct gacaggtaac gatgttacag agcgtctcac agattactct cacaggtttc
    80461 tcgatggttt gagcggagtt gacggtttta ttctcaaatc tgcatcgcca acctgtggca
    80521 taaaagatgt gaaagtgtat cccggggaag gtaaggtaat gtcatccggt agagactctg
    80581 gtttttttgg aggaatcgtt attgataggt tcaacttcct tccagttgaa gacgatggaa
    80641 ggctatcaaa cttcataata agagagcact tcttgaccac acttttcacg gtctcccggt
    80701 tcagagaact ctcgaataat cttaccagga aagggctggt ggattttcaa tctgacaaca
    80761 agctgctatt catgtcgtac aatcaaaacg aaatgaggat tatgggtgca attgtgggga
    80821 agatggattc cagagatatt agagaagtct atgacgagta ttcctctcat ttctacaaaa
    80881 cgatgtcaac cccaccaaga cacacctcgc acatcaatgt cttgatgcat ggactgggtt
    80941 acttttcaaa gtacatatcg tcttcggaaa agagattctt tctcgacaca ctcgaaagat
    81001 atagggaggg aaagattccg ctgagcgtcc ctctatacat tttacgatct tacgtcgttg
    81061 catttgaaag cgaatatctc gcgcggcaga ctttcttcga gccttatcct gaagacctcg
    81121 tcgaaataac agattctgga aagggcagat cgggaaaatg agagcagata gggcccacaa
    81181 gttgaataag agcagtgatc gacgcgggaa tcttgtttta tactggatgc aagcttcaca
    81241 gagaactgaa gagaatcatg ctcttgaact tgctaaacaa aaagcgaaag aacttgatgt
    81301 tccacttgct gtgcttttcg ttctaaacgc taattacccc tcaggaaacc tgcggagttt
    81361 ttccttcatg tttgacggtt tgagagatgt gaaatcggag ttcgaagaac agggaatccg
    81421 cttcgtgatt tttaagggag atccggtcga tatagtcccc aaagtatcta aacgttgtgc
    81481 tatggtggta acagacgtag gttatctacg acaccagata gactggagga agaggatcgc
    81541 tgaaaaacta gaatgtccgt ttctttctgt tgaaagtaat gtggtagttc ctgtaagaac
    81601 ggcaagttgt aaagaagaat acagcgcagc gactttcagg aagaaggtta tgaagcattt
    81661 tgctgaattc gtaagtgatt tcgaatctgt ggaactaagt ttcagagctt cgcttgctga
    81721 atttgaaggt gctacgggta ttgaagacgt tgatctttcc gaccttgacc agagcgtatc
    81781 cattatctcc ggtttccgag ggggtcagaa agaagcaaaa aggaaacttg agatatttct
    81841 ctcgaagaat ctagaccggt ttgaagaaga gagaaatgat ccgaattccg ggtgcctgtc
    81901 agaaatgagc ccctttcttc actttggaca catttcacct tcttacatcg cacgaagagt
    81961 aatcgattcc tgttcaaggg ggaaaaatgc gtatatcgaa gaactggtgg tcaggagaga
    82021 actgagtatg aacttcgtct tctataattc caaatacgat tcctttgaag cactgcctca
    82081 gtgggctcag aagactctga atgagcaccg gaaggacaag agagttcgat ccttctcact
    82141 tttcgaactt gaaagtggta agaccgatga tccttactgg aatgcagctc aaaaggaaat
    82201 gacccttacc gggaaaatgc atggttacat gagaatgtac tggggaaaga agcttcttga
    82261 atggagcacc gatcccgaaa gcgctattag ctcggcaata taccttaacg acaagtatga
    82321 actggacgga cgggatccaa acgggtacgc cggtatcctt tggtgctttg gaaaacacga
    82381 cagagcgtgg tctgaacgcg agatctacgg taaggtcaga tatatgaact caaacggtct
    82441 caaaaggaaa ttcgatatcg attcctacgt caaaagaatc gataatctgt cattcgacag
    82501 aaagtgacga actgaaaact tcagtatcaa ttactttttc atctttcatt acttccagtt
    82561 caagtattag gtcataaatg cctgggacaa ggccaagaat cttgatcgcc tttcttgggt
    82621 atgtgaaggc ctgagtaaca aattccccgc gaccgggtga gtggagcgtg gcgtatactt
    82681 ttatggttct agcctcagta aggagaacgc ttgttacagc aatttcatat cctccagtag
    82741 gtttttctcc ggctgctatt atcaggacgg gttcgcctga tattaccgag gtttctctga
    82801 accctttttc ttccacaaca ccaacggaaa ttcctgattc aatgctcctc tctatctcta
    82861 atgcaaggca ttcatactca agctttttca ccgcatcctc caaaaccgat tcatagtcaa
    82921 gtactaattc cattacggac aacgaatacc ccctttcaaa tgataacaaa aacggcttca
    82981 ttacaaaata aagcccctgt ctagccaagg aaactatctt ctcatagcat tcaccattta
    83041 ctttcagctg aattgatgcc tcccgttcct gctcttcgct ttcaggatcg aatagccaac
    83101 ctgaagcaat aatcacaact ttattggaat cgcttgcaga aagaacggag aaatcaacgc
    83161 tctttgttct agttcctgtg ctttcatcga gtacgagcgg ctcgtaactt gaaagctccg
    83221 tgtagtctac ctgaaaagcg accagaaaag gggaaagaag aacaatgaca atgaaagcaa
    83281 taatcaactt cttcatattt cacctcctta tcgtaagact tgcaatttca cagatttgtt
    83341 tgcacaggtt aattctgagc cgctataatt caaagaatcg tcttcaacgc ttgaaataag
    83401 ccatgagtat ccttctgtat ttaccttcag tgatgtatcc ttttaattag gagcaatcgc
    83461 tccaattaca tggggaggtg tttagatgaa caggcgttta gtttttgttc ttttaatggt
    83521 tcttctttcc ctttcgattc ttattgccaa cacgattgaa ctggagttct ggactcacga
    83581 agatccaaac agaacaccgc ttgaagaacg tttcattgag gaatttcagg aaatgtaccc
    83641 gaacgtagta atcaaaaggg ttacacagtc ttcaacgaag atacaagagc tcatactgac
    83701 ggctttcgcc gctaatcagg ggccggacat tttcaatatg tcaattgaag atgaatttgc
    83761 ttacattgca aatggcagag tcgcccctgt tgattatgaa gccgcaggtt acaaagacat
    83821 ggaggattta ctggcatcat tccttcccgg aactctagat cctgtgaagt atgaaggcga
    83881 gatttatggg cttcctctcg aaattaccaa ctggtgtatc ttgcttaata agaaagtatt
    83941 cagagatgcc ggtctagacc cggaaatcga ctacccaaaa acatgggaag agatgatgga
    84001 agtatctgaa aagcttacaa taagggaggg cgagataatt acccgtaggg gctttgattt
    84061 caggtaccca tactatctcg tagcgttcat gccattggtc gaacagatgg gcggaaaact
    84121 cgtaagtgac gacggaaaaa ccgcgattat aaatgacgaa gcgtggctga acttccttga
    84181 ctatatggca gaatggggac cgaatggaag aaatctcggt tctccaactt accaaaacgc
    84241 tcggaagtta ttcaatatgg acaacaacga cataggcatg tgcacaaccg gtctatacca
    84301 actggctaga ataaaggccg acaatccgga gttctatgat agtggagaat tcatggtagt
    84361 tcccttccct cagttcgaag atgccgtaaa ttatgtgcca gctcactatt atggacacta
    84421 ctacatggtg aactcccaga agccaaaaga gaatcaggaa atggcctgga agttcatctc
    84481 ttacatgctg agtcatgccg aggagtatct tgaaaaggtt gcgatcataa taccaacaaa
    84541 cgaccttctt gaatcagaga cattcaagaa ttatccatat tcagacgtct ttatctccga
    84601 tcttgaaaaa tcgacggttg tgtatttctc tgagagctca gcaaagattc agtctctaat
    84661 aaaggaagct gtagaatctg tcatgcttac tgggacaagt tcccaagacg ctctaaatac
    84721 actgcgcaga aaggtccagg aagtattaga cgatcagtac tagattattt gcccgcctcc
    84781 ggcgggcaaa ttcctttcag aaaggagtga gccttacctt tgagacgtgt ttttaacagg
    84841 ggcatagaac acaaaaaagc aaggtggggc tggttctttg tgatcccatc gcttgctttt
    84901 tttgctgtct tttcgttcta tccaataatg aatgcctttt acaccagctt attcagaaaa
    84961 agactccttg atctgcggcc cccaaagttt ataggctttg caaactatga gtatcttctc
    85021 aaatcgcccg atttctggaa ttcagtcaag gcgacctcta tcttcacact tggaacgttc
    85081 attccaattg tggtatttag tcttctactc gcggtcttca tcatgtcaag gcgtaggttc
    85141 caaaagttct tccagatagt gtactactca ccggccgtcc tctcttcagt agttgctgca
    85201 gcgatctggt tgttgatatt cgatccgcga ggtctggcga ataccaccat gaacactttg
    85261 ctcggaaccg taggcaagga ttataagtgg ttggcaacag cgggaatggt aaggttgtcg
    85321 acaatcctgg tatatttctg gaagtacata gggtacttca cgattattta tattacagga
    85381 ctggcagcca tacctcagag cctacacgaa gcggcacaaa tcgatggtgc aaacggctgg
    85441 aaggactttt ggcacatcac cctccctttg ctgaaaccga caaccgtttt agtgtcgatt
    85501 atgtctatgc ttcagtgtct gaaaacattc agcacccagt atctcttcac gcaatcggga
    85561 gctcctaccc agccaataaa cgtaataacc cttaacatat acaacacggc gatcagagac
    85621 catgacattg gcagagcaag cgccatgagt attatcctct tcggcataat gctcttcttt
    85681 acctggcttc aattcagagt aagcaaatca ggtggagaag tagactatta ggaggtgtct
    85741 tgtgaagaca attcggaaac aatccgtggt tgttgatgtc ttgatatggg ccttccttat
    85801 agtgacagcc atcgtgattc ttgcacccat agtctttatg ttcacggcct cgcttatgcc
    85861 ctcgaaagat atcctgaaga tgccgtattc ctggatcccg aaggatctct acctggataa
    85921 ttactggcaa gcaataaggg ggaatgatgg cagtttcatc taccccagga acatactcaa
    85981 ctcattcata gttgcgggag ctgtttcact ctctaccgta ttcctttccg cgttgacggg
    86041 atatggtctg gctaagtttc cctttaaagg gagaaatcta gtgttccttt tgatcatggc
    86101 taccatgatg atccccttcg aagccattat gattcctctg tatctcgttg caacatcact
    86161 caagatccag gactcatatg ttggactgat actccccttt atggttaacg cttttggaat
    86221 attcctgatg aggcagtatt tgataacgtt tcccgatgaa ataattgatg ccgccagaat
    86281 agatggttca ggcgagttta gcatcttctg gaagataata ctgccaaata gtgctccggc
    86341 agttgctaca cttgccatcc ttacatttcg aagtcagtgg gacaacctgc tttggcctct
    86401 cttgatctca cagagtgaag aaatgaagac aatcccactg tacattgtga agtttgccat
    86461 ggaaaaacat actaacgagg gagcgatgat ggcggctgca gcaatagcaa gcattcccat
    86521 gctgattctc tttcttgcgc tctctaagta ttttgtcagt ggagcgtctc ttcattcatc
    86581 gagaaagggg tgatctagga agtacctagc ctcaacaagt ccaaaaaagc tgcccttaag
    86641 ggaaaaaatc tcctgagagg tgtttctgtc aattacagca accgaatcat caagccgaca
    86701 tacgaggagc ttattgtggt tttgccagtc gattatcggc acggtctctc ttaatttgcc
    86761 gtttacgacg tgtctttctt taagagaaaa gttgtttgtg tcaaaaacca gtatttcatc
    86821 aaccctcgag tcgaccacaa acaaatcccc atcactaact gaaacatcca ttgcgtagcc
    86881 aaccttaatt tcggctttct tgacgagagc agagtcacca atttcaaaaa ccattactcc
    86941 tctctctcct gctgcaacat aaaacgagtt ttcccaggct gtaatggcgt ttactactac
    87001 cccctcttcc agatcactca catacctggg ataaatgtga tcgattagct cgaatccctt
    87061 ctcccctctc cgaatcacga aaagtccatc aagctcgctt ccaaccaaca agtagtcacc
    87121 caggtacgct agggagttta cccagccttt caatggtatt gtctgaatgt gtttataaaa
    87181 ggacgatcca tagaacttaa agacttctac cccgtgtgtc ccgtcggcta caaagacttc
    87241 tagcctctca atattaactt cgattgcttc gcaatgaaaa gtgtccacca ccctataacc
    87301 ccccggttga agaacgggaa tcaccgcaaa gcctttgtct ctaaggacat tgaagatcag
    87361 tttaccggca actattcctc cactcgataa cgaggtgaca ggtatgactc taatattcca
    87421 ctcctgattc ccggcaatga cttcaactag accgttaaat ccagatccaa tgtagaaccc
    87481 actagcgagt gaaatagaag agaaaaagaa aacgatccct aagagaatca ctcttctcat
    87541 attcccaccc ccaagaccat tttaatacac catcaaataa acaaggatcc tatctaacga
    87601 cacaagactg actttttggt ggtctcaaga tcggaaatta gatttttccc tagaagaaac
    87661 aagtaatata attgaataga gaggtgatct tatgagtaag aaggagttcc ttaaaaggat
    87721 tctttcagaa gggcgaagtg cagacgagat agccgatctc tttggaatta ccgccgaaag
    87781 gttagagaat tatctggaag acccttcacg gtcgaagctt gccgtcgcag agatcaagac
    87841 tgagctaaga aggctggaaa gcttgcctct ggaaacaaag gaagacattc ttctcttctc
    87901 tttcagagtt atactggaat cgataaactt tctgttctcc gatattgaaa gtgctgcttc
    87961 ggataaaaga gaagagagac tgccgcgcct gataaggaag ctcatcgatg gagcatacct
    88021 ccttctcgcg atttccagac tcgagaaggt cggtaagaac ctctcatcga tgagattcga
    88081 cgagaggttc ttgcgagcat gccagaataa ggcattagta gtcgatagat taatggatct
    88141 tgccgagctg atcgatcggg aaattgagcg ttagcctttc cttttgcgat tctccaatcg
    88201 gtttcatcaa gtgtagtata atcttcttgg aagtcacttt aagtgaggga tatgttttgt
    88261 ctgattctcg tcaacgggaa ttcatttcat ttttatcggc cgaagatgtt atcgaaaaag
    88321 ccaatgagag tcttgagggt aagaactgga aaccgctagc tgagacctca aacgctgcat
    88381 tgatttcttt gaagactcca ctggtcgcaa gtttcttcaa gaagttcttt gagaccaagt
    88441 acgctttcat ttacagaagt gtagaggatg aattgatctc ctcgagttca gagaaactct
    88501 ctgatcttgt cgacactctg tttcaagcgg caactgagcg acagactcta gatgatagtt
    88561 tcttcgtttt tttggactac ttcaaggagg ctcctcaaat tctagaaaag atcgtacttc
    88621 actattcctc tttgatcgcg gataaatccg actctctctt aacgagtcta agagaactca
    88681 agaatttgca cgaaagggtg cggagaaggg ccgatcaatt tggactgaat ctcgctttga
    88741 atgaaattgt ttcgaggttg tttagcctcg aatgcccatt acttcctacc cttgaatcag
    88801 atcttgtaga tttcctttca aaattccctc ccgatgaaga gggggcttct aaattcctgg
    88861 acaatcagat tgtgcttgct gtgggtgaat acgatgaaag tagtgtggag gttcagcttt
    88921 cgagattgga gactataatc aaagaaataa agagatattc tttaaccttc atgaaatggc
    88981 ttaccgttcc cgataatctc ttcgtttcat acctcgaaaa gggttttaga aggtacggga
    89041 caattgcaac atggcttgaa ggcataaagg aagcgaggac cgagtatgga cttggtcaga
    89101 gtcattggga acaagcaaga aggtggtatt tcgaaaagat atctagcaga attctaccct
    89161 ctttccagaa ggcaatggct ggctgggctt gctggacgat tattgaagaa cgacagatta
    89221 aggacccttt agcccatgcg ggcgaaacta tgcgttactc tgctagaagg agcgagttta
    89281 ggattataaa atgtgaagaa agcagcggtg tttttgatct ttgctctgtc gacgaattga
    89341 tgataacttc gtcgggtctc aagaagggtg aaagaagctt tccgttcaac tctataagaa
    89401 ctcagctaga agagttgagg ctctctcggg aagccttttt ggacagcaaa gatgcaattt
    89461 tcgtcggata tacttggaag tacttacttc ccggaggggg gccagacaag gaggccaaaa
    89521 acaacgagcg gatagagtgc ttcaactatt actgtgtaac agtttcggca acgggtttcg
    89581 gtgtcccttt gataaagctg ttttttgcaa gagaggaaag cgcaagaact ttcattgagt
    89641 tcttgaaaga agcgcggata atgtcttcgt ggaaagagta tcctcagaat gggctccccg
    89701 ctccgctaaa agcaaaaaca aagaatcgat ctgagtctga agaccagatt aatcctggag
    89761 aggctaacca taaaacagtt gaggaactca taatacaaaa tatggtcgaa gagagtgatg
    89821 aatcggaaat cgaagagaca acagaggttc ccgtggtccc aatatctgca gaactcctcg
    89881 aaaagatcaa ctcacttcat tacgaacttg aatggtcaac caaaaaaatg aatgaggtag
    89941 agtggacata tttctttcca gagagggaaa aagaggaaga agacataaaa attgcagaac
    90001 ccttacaaga agagatgact ggattcagag agcttgaagc aaaaatagaa gccctgtcgg
    90061 ggaaaacaaa aagaaccgtc tgtaaaatcc tttcatgtga acccgaaaga gagtattcgc
    90121 ttcaagaact tgggctaaca ttctggagtg tctttgtagt ggcacccaaa gtgacaagtg
    90181 gcggtaaacc ccttatagaa gttattagtg gtgatgtacc caggcttgtc tggggaaaca
    90241 atttcgatga agaaactagg gaactgctga aagcgaggtt cgcatcagat gattgacaca
    90301 aagctgaaag gagttttccg tggattacca tccgatgttt gaggcgataa gagccggaac
    90361 tgccgtcccg gttgatttcg tcaccgagat actcacaggc cgagaaaaat acgttcaaga
    90421 gattgagaac gatatcaact acattctgac cgggaaaagc aaggtgagag tctttctggg
    90481 agaatacgga ctgggaaaga caactcttgc cagatacgcg gaatacgtgt ccagggaaaa
    90541 aggaatgatg atatcctgcc tttccgaaaa ggattacgac acactacaca agcaggacga
    90601 attcttcaga tcaataatga agaacatgac aatggtggga ataaacggga atcctttgaa
    90661 gatattcctc agggcctggg ccgaaaagac gctcggcgag cttgatgaac tgagaattcc
    90721 gtctgacgat gtgaactcag tgagagaata cctatcaact agagacaatt atgaccccga
    90781 tggcatgttt tctcatttct gcgccgcata tgtagcaagc gctggacagg gagcaccggg
    90841 tgaagagttt tatgcgtatc tcgttggaga tagtgttgac aaaagaacga tgaaaaagca
    90901 cggcatatac cattttctcc aggatgatgg ttggaacttc ctgaagtctt tcacttcttt
    90961 gatggatact ttacaggtcc cagggttggt aataataatg gatgagctcg aatccacaat
    91021 gaacagaaga agagatgtaa gggataggac cttcaatcaa ctaagggaaa tcatcgacaa
    91081 gatgtctgct gggttcctcc aaaagactta ctttttgtgg cttggaacag atgtttggtt
    91141 cgaaaacaaa cacagaggaa ttgcatccta ccatgcgctt tacgacagga tgaaaaacat
    91201 ctccggggat aatagtggaa agaccattat cctgaatcta gaaccgttgg gaaaagaaga
    91261 tttgagacag ttgatcaggc ggttaaagac gctttacgcc agctgctatt catacactgc
    91321 agactcgtcc ttcgaagaga acacactgca gtctctggtt aagcagtaca caggaattga
    91381 tgggaaactc agaccctccg ttagagaagt tgccaagctc accgtagagt tcctcgattc
    91441 tctcaggcaa ggcaaaggac aagaaggaaa tggagacgca gattcagtta aagctgctga
    91501 tcaactttgg tgattgatcc cagactaaat cagaagcttg tggatctctt ctcttataga
    91561 tatcgctggc agagcttgag agagattcag agcaaaacta tccctcctgt gctggatgga
    91621 aagaacttgt tgctgattgc gggtacggcc tcaggaaaaa cggaagcagt aatgataccc
    91681 gtagttaata gactacttga gctttctgct ggtctgaaat gcatttactt cgctccactg
    91741 aaatctttga tcaacgacgt ggcctctcga cttgaactca tactgagacc ttttggtatg
    91801 gtagtgggga agtggcacgg tgatctcaca aaatcagaga agttctctac tgcgaaggag
    91861 gcctccatac tcgtaacgac acctgaatca gttgaaggta tctttttgag tgagaacata
    91921 gatttgcttt caaagataga attcgtgata atagatgagg tgcatgcttt catcgattct
    91981 ccaagaggcg ctcagcttgc ttctttaatg gaaagaatta agattctctc tggcattgat
    92041 caacagagaa ttgcaatgtc cgcaactgtt ggaaaccctg aactccttat ggaatggctt
    92101 agtgggagct caaacagaga atccgaaata gtaactgacg acagtaagag caagcgaaca
    92161 attgaggttc tcacggaagg agagatctct ccagccgaat acctcgcaag gctccttgag
    92221 gagactgacg acaagattct actcttttct tactcgcgag cgaaggccga ggattttgca
    92281 gcttctgcaa aagcgttagg actggctatc ccggttcacc acagttccgt atcaaagagc
    92341 ttaagggtag agattgagga ggagtttaag agggacaaga aactgagggc tattgtagcc
    92401 acttcgacgc tggaaatggg catagacata ggcgatatag atagggtgat ctttcttgag
    92461 atacctccat cagcggcttc attccttcaa agagctggcc gtgccggaag aaagaagaac
    92521 aaatccacaa tttccgtttt catagaggat cctcaaagcc tctataatct tctgggaata
    92581 ctcgatatgc tagaagaagg atcggttgag ctactcaagc ctagcagttt tcatcttcct
    92641 ttgctgggcc atcagttgat cggcctaacc aagactgccg gttctctgtg caaggaagat
    92701 cttacagtgc tcaagagagc cttccctttc cgacaaatcg gcagcgcgga tttcgaaatc
    92761 gttttggatc acctcgttaa ggaatccttc ctggcaagaa ggggaaatgc gataacttcg
    92821 ggggctgcta ccgccgagat gatcggaagc ggaaaggaaa agatggactt tgttgtcctc
    92881 tttcccgggg gatttgaata ctccgttatt ttcaacggac acgaaattgg aaagattcac
    92941 cctgcagttc ttagcacttc ctctgacgat gaagtcagtt ttcttctcgg tggaagatca
    93001 ttccttgtaa aagaagtcaa tcctgccagg aagacagttc atgttgtttt aggtgacagc
    93061 ggcaaaccgc cttcttggtt tggaagcagt tcgttgatga caaaggagtt cgcaagggcc
    93121 ataaaatctt ctctggcaga gactaagatt cctgatggag tggttctctc acaggacgct
    93181 tcacaaacgc ttcttgactt caccgataat cacagggcat catctatttc aatttccctt
    93241 cgaagctcaa gaaaaagtgt aataatcgaa accttcgcgg gcgatatgtc aaacatcttt
    93301 ctctctctgt gcataaaggc agtttccggt atcaagaaga ttagctctag ttggcacagt
    93361 gtaatagtaa aggacggaat cgatatcgat gaacttcacg aaatgttgct cgcgatttca
    93421 agaatggaag aaaatgagat caaagggatt ctctcaacct atctcagaga aaacccaaat
    93481 gagctaaaga aactgtacga actcttaggc gaaaaactcg atagattcgt tcctcaacaa
    93541 ctccttataa agtatgtaat acacagattc ttcgactccc tcttactgaa ggaactgtct
    93601 gaagccataa tggtaacaga tgctgactcc taaactccct acaagcaaaa cacccactat
    93661 gtaaaagaga aaagggggca gttgaacccc ccaaaaatga atctaattca gtcctcctcg
    93721 aaatcctctt ccatctccac aactgaatcg ttgagccttt caagatcacc tttcagttcg
    93781 aggatcgatg cttgaagctc gggtagacgt gcaatgatct cgtcaagtcc atcaacccag
    93841 tcttcaatgc ttgatcttaa ttccgtcact tcatcctgtt ccatatccgg cagagcctcg
    93901 gtaagctcct ggacatcagc attctcaagt tcatcgaaaa gctcttcaac tagatcgatg
    93961 atcttctcta tgttctgcac ctttgccact cctttcgggt ttgacttcta tactgacaga
    94021 tgttatcaca acttagtgga gttttgtata gtgaaaaaag catgctttcg atcctgaaga
    94081 ctctagaatt acgcctccat gatcagccca tttttccgtc gctctttaat cttgctcaaa
    94141 agcaacgaaa gaaatactga tgatttggga gatctgaaac acaaaaaaga agcggccgaa
    94201 acggccgcct ctgaattcag agaaatacaa ctatctctct gggaaaccat aaccgattgc
    94261 ttcgttccac caatcgggat atggtgagga tttgaaattg acatatccga gtgcgtagtt
    94321 tccaagaagc ctgtcaccaa gaagtttcca ttcttcgtgc catgcaaccg cgttcatgaa
    94381 agcatagtta gagacgaaat ctatagcagc ttctggatcc gtctcataca tatcagtagc
    94441 tatcttctga acttccggtg taaccttgta gagaacttcc agtttaggat ccctgtagtt
    94501 cctgatatcc tggatagcga ggttatagtg aagttcggcc atctgctgca cataagagtt
    94561 gacccaccaa ccagagtctc ttctgaactc ttcgtatctg ctaccggtct cgtagaactt
    94621 gggaagctct ctcattatcg gccacaatgg aactatgtag cttgtatcag gcgcgccgta
    94681 accaaaccag ctgattccct tgaacggttc tgcataggca gagtttgtct cgcctatgtg
    94741 gacatagcag gttctgtgca tattgatact tctctcccaa gaacccgtct taccagtatt
    94801 ggcatatctt agaggatctc cccaggggcc cgcagccagt ccaactgtca ggtcgtaatc
    94861 cgttccctga tagtaatcac ccttgatcgc aaagatatca tgtacggtga gaagtctgtc
    94921 gggcttaaca gaaagcggga atctcaagtc gtgtgggctg agtcccagag aaggagctac
    94981 gaggtcgaat gctctccact ctcttcttgt tgcgtaaagg ctgtcgttcg gagcgtaaat
    95041 ctcggctatg ttgaatggct tgcctgaact gggatcatac catccctgtt ccacggcaaa
    95101 ggacactatg ttcggggaag ccataacgtt ttctgtatcc tcgagatcaa cttccatgat
    95161 tcttgctcta tttgctgcaa ctgtgaagtg gtcacttgga atccttacac cagcccaaat
    95221 atctcttccg tagaactcaa cgatccaggt ctcttcgccg tcggcaacgg tcattgtctc
    95281 tccgccgcca ccaaccgtgc tccatccata ctgctcgacg agatcaccca tgatccttac
    95341 agcttctctc gcggtcgcgg ctctctcgag agcaatatcc tgtgccgtcc agcagtcaat
    95401 gattccctta gagtccgtcc agagaacctt tcttacttcc ttgccataat cggtactggt
    95461 gtcgatacca aatgttgatt cgcttacggc aactcctttc tcgttcatga aggagtatct
    95521 tgagtggaag tactgatagg tgtgaggaac ctgaggaatc tgtcctatga gagtcccctt
    95581 cgaaccaatg ttcacttcgg gatagttgcc gtaatcgatg tagttgtgag aatcgagcac
    95641 gatatctctc attgaacctt ccggccaatc catcgcaggg ataatccaca gacggaaatc
    95701 cgcactggtt gaatcatcgt tgtgcgtaat gatagttgaa ccattaacca ttgcatcctt
    95761 gccgactcca aggatagtac atgcaacggc tgcacttgcg aaacttatca atgccacaat
    95821 cagaattatt aaaactttct tcataagagt acatcacccc atctagatta gatttgttgt
    95881 tgcttttgaa acattactac tacgttgtct gactgatccc acctcccgta tagattcact
    95941 gaacaagaaa gggtttttgg attctagttg ttcaaagagt ccagaagttc gccccattct
    96001 ttaggcagac ctgttccaaa gttaatcctg tctgcacagt atttgcccac caagtaatct
    96061 ccaaacttta gccagtcggc ctgccatgca accgccgtat tgtaagcata atcacttatg
    96121 atagcaagcg cggcatcctt atcggtctcg tatagttctg tggcaagttc ttgaatcttt
    96181 ggcaccgtct gatacactgc ctgcatcttt ggatccctga agtttcttag atcaacaatg
    96241 gcatctttga atctcagtct agtcatttcc tgaacatatg tacttgtcca ccatccagaa
    96301 tctcttctga agtcttcata tctcgagcca acctgataga actctggcag ctcagacatt
    96361 gccggccaaa gaggagtatg gtactgtgtg ccgcctccac catatccaaa ccatactact
    96421 gatccaatta caggcggcaa ccagttcttg atctgactga tctgaacata acaactcaag
    96481 tagataccta tgggtctctg ccttcctccg actctgtagc ttgcaaatgg atcgccaaaa
    96541 ggcccggcac caattccttt agtcaggtcg aattccgttc tttcgtacca atctccagaa
    96601 attgtgaaaa tgtcccaaac ggaaagtttc ttatcgggtt ttatccagag agggtagtgg
    96661 acatctaggt actcgtaccc catggatgga gccatcaagc tgaatgctct ccattcgcga
    96721 atgtttccgc tgctcctcgg ggcatacaca tctgccggtc tgaagggttc tccggaattt
    96781 ggatcgtacc atccctgttc aaccgcataa gaaataagat tcggggagta catcacattt
    96841 tcagtgtctt caaaatcgac atctcgaatt ctcatcgtgt ttgcaccgac gaaaacacag
    96901 tcgtccggca tcctcactgc aacccagaga tcacgtccgt agaattcggc tatccagacc
    96961 tcatctccat cagttacgtt cattatctcg cctgaaccgt accaaccgta ctgctcgaca
    97021 agatccccca tgattctgac cgcttctctt gccgtggagg ccctctcgag agcgatatcc
    97081 tgagccgtcc agcagtcgat ccaacctctg gcctctctca gttcccttct aactccggca
    97141 gtggactcac ccattgcaac tcccatttct ttgataaacg agtatctgga gtggaaatac
    97201 tggtaggtgt gctccacctg aggaatctgt cccacaacta ctcccttgtc accaacatca
    97261 acgaagggaa agtcaccgta atcaatgtag ttgtgagaat cgatcacgat atctctcatc
    97321 gaaccttccg gccagtctat agccgggatt atccacagcc tgaagtcggc cgaactggaa
    97381 tcgtcgttgt gggtaacaat tgtcgaacca tccaccgatg catccttgcc aacggcaagt
    97441 atggtacagg taagcgcggt actactcaag aagaccagca aaatcacaag taccaggaac
    97501 gctttcagct tcaatttcac gttttgcccc ctagaaaaag gattgtggtg ccttcctgta
    97561 tcagttgatc ttcagacttt cagttctgga agactctgga tgggtcggct ttgcttggat
    97621 catgtagata gtaccccgac ccattactca taacatcgac gtagttcgga aaagtcacgc
    97681 taattgcctt gtcagggaag aactgattca tccagttcaa ggcctctagc gttcctgata
    97741 ggtgcctacc gactctgtaa tccatcggtg taccaataat catgtttcca agagcatccg
    97801 gaaaaccttc gtatatgtcg taatcgcagt aaactagacc cttttctgca gctgtctgaa
    97861 ggaattcatc tttaccctct agcataagtt cctctgacat gcgcccaacg actctgtcga
    97921 taaaggcttc gggcgtctcc atcaatattg ccaacgtatt gctgaagtcg cccacttctc
    97981 tatgtgtcag acctctcaag ttttttggcg aggcctcgca cttcatcggg aactgagtgg
    98041 cgcttagcat cattgcggcc atcatggcaa tatccattga cctgtcgtgt gccacgtaag
    98101 tacttaccac cgggtacatc agtgatgcct cgtgaaaatc gaagaaaatg tcaatgtcct
    98161 cattccgaat caactccata atcgcatagg atattctctc cgtaagcgtt ccatccggtc
    98221 ttccaggaaa agtcctgttc aaattcctga tgtcctggta agccaaattc tgggttgatg
    98281 ggtagtgaac gtaggtgaaa gggtctggcc actgatctag tggattagtt cctcggtcac
    98341 cgactctata cttcttctga ccccatggag tctctatatg gtagtacttt atgtatgcgt
    98401 tgccaagcat tcccaatgta gaggcactca tatttgcatg aggaattata tagactttcc
    98461 ccgatgtcac gtcgatgttt tccatcataa cgtaggtcgc gagtgtcgtc gccggctcat
    98521 atggatgggt tccaccgaga acaagcatcg agcctcccgg tactccggag tcatagatat
    98581 aaataggtgt atctccccaa gtattcttga gacttggaag atactggcta agcataatct
    98641 tttcagagaa gtcttctgaa acgacgaccg tttctttgta atgccggagt tcccagaact
    98701 ctattccagc taaaaggccg agtacgcccg aaagaaccag gaataggatt tttagataca
    98761 gtggattctt catctcacac ctcacttaat cccgattact cttagtatga agaccatgat
    98821 aacgtaggaa tagactatgt catagacaag gccttttcgc ctttctcttt tgaaaagatt
    98881 ctttagcaga aggattgcaa agaacccagt cattacgaag taaaaatagt gaataatcgt
    98941 atccattaag accaacatgt cctcacctcc atcaatagac tacaaggaaa tctaactggt
    99001 tggggaaaat aagcatcagc agcccgatag cacccatgaa aagccacggt atagctatcg
    99061 ctttgaggaa agacatgtag ctgccttcat atcctaccgt ctcaacagta agccttccca
    99121 ctattctcga tggaggcaga cagtcgccca atgggaaaag gagactgagg gcagcagcag
    99181 taactgtgac attcagtcca accgaattga ataggaagat tattggcgta ccgaggataa
    99241 ttgcacttcc gtagctgagc gctccttggg cgaaaggtgc aaaaagcagc gcggtaatgt
    99301 agatgaaaac catcggtaga gtaatgaaag tgatagcaag caagcctctg acaccagttg
    99361 ccgtcatgat atttacaagg gcacctacgc ttataaccgt tgctagtagc ggaaacacct
    99421 gatccatagt ctttaccatc acatcgaacc atttgcgaac atcccacctc ttcctgtcca
    99481 tcatggcaga ggcaaaagca catatcacaa atgtaagcgg aaggccaaga acgggaatag
    99541 aaaatgccgc gaatttagat agtagaatca acacaagcag aacagcaaaa ggagttatga
    99601 tcttgaacca attcatccct tcaggtggaa gtggaagttc ggaaaggatg tgttctttgc
    99661 tctttctctc cggcgtcttg ctagtgccag tatagactat tgtaaacaag gcaataatta
    99721 ctattggtac aagcagtacc acattgaaac caacatatgg catgttagcc tgagctgcca
    99781 tcaacattgc ccataggttt ataggaggtg aaacggcagc aagcattgca aacaagtata
    99841 tgaaggccgc cacacgcctc ttcgaaaacc ccatgtagct catgactgaa gctactaggc
    99901 ctcctacgac aaatatcgat acgctccccg ctccagtcaa agcaccagga atgatcatca
    99961 atattcccag tagcactaac attagccact ttctgtcgta aaactgcttc acaatccatc
   100021 gaaccagggc attcatagcc cctgtctcag agtaaatatt gataaaaatc gatgcggtta
   100081 gaaaaatcat tccaacatca aaatatgtaa acataccctc cactagaagc ctcgagggaa
   100141 atccaaaccc acccactaac gcaccaacaa tggcggtaat taccatggat atctccggcg
   100201 atttcaactt ccagcttgca agagcaaaag tagcaaccat aattattgcg ataatcgttg
   100261 tctgaagata catttttact cctccaaaga agcggcgatc tggctagaac gccatcattc
   100321 ggccgggaac atcaatttaa ttacctgcat gaaatcaatc gtgttgtcga tgagagtgag
   100381 ggggatatct ttctcttcag ctatcctagt gaatttacca tctaagtttc cctctttcat
   100441 cacaattagg tagtctgcat atggtgcaat cgcatcaatt gaccgttcgt ctgcacttcc
   100501 gggatttccc cttcttgcct ttccttcaat gtgagcggca ataataaaca tgttgagtct
   100561 cttggcttct tctatcatct tgttgagtct cactatctct tcatcaatag ttatacccga
   100621 tgcgccaagg ccctttgcag aagaaccgat gattacgaaa agcatcttgt attctctcgc
   100681 agtaagatcg ttcggatttt ctttaagtgg ttctgcattg taatcgaaat ccgtgtcttc
   100741 aattgctccg ctctgccata ttagaagcct tgccattttg ccgccgggac cctgccccgc
   100801 gttggtgaga agaaacggta gttgaccctc aactactggg tctccagata caaccgcaaa
   100861 tagaccagtt gtgaatagaa ccatgcttag taagaccaca atcaatacct ttctcatgat
   100921 tttgcctccc gaattatgaa agtgaagatc aaatacaatg aaaagaaaat caccttccaa
   100981 aagtgcctta cggtgaatat acattctagg agaatatacc acactaacat ctctttaacg
   101041 ctgctttctg tagccgaata ggaaccaaag ataggcaatt aatctcgaaa atcgctgcga
   101101 gactcagact ttcgtgcgaa atctacttcg caatacgaac aaactaaaag cgacaatgaa
   101161 ccgtaatctt aggaactgta ctgcatttcc ttctgagcga accgggacct ttttaagaag
   101221 acgcacctac agatttgctc tactctctgc agtacagcca gtgaatcggt tgttactatt
   101281 tccttcaagc tccacgaaat tcttcataga aaaatcattc gaacaaaaca tcactgtaga
   101341 tctaagacaa cgaatagtaa tgatacatca tttcatcttc agagaatgga catttctggc
   101401 cgctcgagac tccaaaatta cctctagatc atgtccttaa gatcctcagc gacaaatagc
   101461 cgagcctctg ttagagactg tacctcgtca actgtcacct cacttgcctt ctctttcaac
   101521 agaagtcctc cggcaaaggg ttcgattacc gctaactctg taacaataag gtctactcgt
   101581 ctgtttgaag ttatgggaag agtgcattgt ttcacgattt tcggatttcc cctagatgta
   101641 tgtgtcatgg cgacaataac acgtttagct ccagtcacta ggtccattgc accgcccata
   101701 cctggcacca tcttgccagg gaccatccag ttagccagaa tcccacgctc atcgacctga
   101761 agacctccta ggacagtgac gtcgagatgt ccacctctga ttattgaaaa agacatggca
   101821 ctgtcgaaaa ctgccgaacc cggtagagcc gttacatatt gacccccggc atttgtaaga
   101881 tctctattct ccattccttc ttccggtaat ggtcccatgc cgatgatgcc attttctgat
   101941 tgaaaaaaca cccttatacc cgctggtata tagtttgaga ccagggtggg aattccaatc
   102001 cccagattta ccagatctcc gtcagccagt tcccgtccaa ttcttcttgc tattctctct
   102061 tttgggtcca tcattgcact ctacctccga caacaatgta atgaacgaaa actccaggaa
   102121 tatgaatttc atccggcgac agggaaccaa ttggaacgat ttcttcaacc tctacaagca
   102181 ccgtatcaca tgctaggacc atcagagggt tgaaattcct tgcagtcaaa gagaatacca
   102241 gatttccgaa gtaatcggct ttcttggcct tcaccaaagc aaaatcggct cttagaggaa
   102301 gttccatcag gtaatctctg ccatccactg ttaaaacctt ttttccatcc tgaaccacag
   102361 tcccgacacc tgttggcgtc agaacgcctc ctaagcctac accaccggct ctaattctct
   102421 cggccagagt accctgcgga acaagctcta cctccagctc tccgtcaatc atctgacgtt
   102481 gtgtctcagg atttgttcct atatgagaga caacaatctt ggaagcacac ttgttgacaa
   102541 tgagttttcc tatccccgtt tctggaaagg cagtatcgtt tgctacaaga gtcaaaccag
   102601 agatttcgtt cttaacgatc tcatcgataa ttgagtctgg agaaccacaa cctagaaacc
   102661 ctccaatcat cacagatgaa cctttttcaa tcagtgagac gatctttttc attcccacga
   102721 ctttcaaaga aatacctcct tcggagtatt agtaagtctt tcgaattatg cctgataaag
   102781 gcatatgatt atcttaccaa aagctccaat taggaacaaa gatatcgcca cttctgataa
   102841 aaagaacctt tgagattgta agcccgtgca tatagatttt ttacgaataa ctttcttttg
   102901 tacaagagat ctcagccgga acgcgcctgt attgaatcgg ccgtgctttc ccatttccca
   102961 gagtagaacc tgataaactt cagtgttgtc ctgaaggcca tatcggccat cattcctatc
   103021 caagcaccaa atagaccgag accgaaatat cttacaagca cgtagccaat aggaagtctc
   103081 aagaaccaca ttgccacaag cgaggtcttc attggaaaag aagtgtcacc catgcctctc
   103141 aaagccccgg tgaccacgta atcggtcccc atcactacct gaaagagccc gataattctc
   103201 actggcagtc gtccctcttc tatgagcgac ggctcatttg tgaaaagaga gatgaaaagg
   103261 tccggaagca acgtaatggt taatccaatc gcagaggaga ttgcgagggc aatgaaccaa
   103321 ccctgtctaa ctacaccaat cgacattctt ctttgcctgc caccattgta gattccgact
   103381 aaggcggtta tagcaactcc cataccccaa gcaggcatga aggagagaga ttctacctga
   103441 ataccgattc tgtgggcagc atacgcctgt ggaccggaaa taaagaggat atttgcgaag
   103501 acaagaactc caagagagaa tccaaagttt tcgatggaag ctggaagacc tagaacaaag
   103561 atctctttga aaagccactt agagaatctc cggggcttcc ttgacatcga aattcttcga
   103621 tttcgaaaca gcaaaacaat aattatcact gaacctacga ctctcgacaa gactgttgca
   103681 agtgcggccc caaaggcacc catctcgggg aacccaaact ttccaaagat cattgagtaa
   103741 tcaagaaaga cattcagtac atttgcaaca gcggctacta tcagcggcga tttcgtgtct
   103801 cccgctcctc tcaaagccgc accaagaaca atcattatgc tcattgcagg gaaacctgcc
   103861 atgatgattt ggagatattt gcttccgttc atctgcatca acgagtcgct agaaggaaag
   103921 agaactgaca acaagctatc agagaaaaaa gacccaaaag aaagaatcaa accagcagca
   103981 atgctaagat agatcgcatg ccatgcaatc aaatctactc ttctgtgatt gtttgcacca
   104041 taactgttag aaagcaacac catcgaacct gttgaaatcg ctactaggac tgcctgaaat
   104101 atgaagacaa cctgatttgc ggtaccgaca gcggtcataa ttctccagtc ataatggccg
   104161 aggaaagcgg tatctgaaat tccaagcagc atctggagta cgttttctgc catagcagga
   104221 atggccatca caagaaggga acctcgaatc tcctttgctt cttcatcgtt tagataagac
   104281 ttgaataggc tgtaagacat caacgcctcc acagtaagga atccgtacta tatagtctac
   104341 catgcagttc aatagtttaa aagacctggc gaacaataag aaccaactct ctttgaaaac
   104401 ccgggaagca aagtaaaatt atacttgtga aataactcaa ttacatattg attttatttg
   104461 atttccatgc tatattattc aatgtttatt ttgtaatttg tgattaagaa tgatatataa
   104521 ttaatttgaa tcgctgttat tcaagtagat aaaccctttg tttagagatg tggacgttta
   104581 gtggcgcaaa gcgccacatt tttttgctta tatttcattt tatgtttgtt ttctggacgt
   104641 ggtataattt ctcgaaaccc gatgctatca gttcaatatt gttggaggac acttatgaaa
   104701 cctgaagata tcgagagttt tctgaattcg gttctacatc cggaagaatt cgacgattat
   104761 tgcttcaatg gaattcaaat agaaggaagc aaggagataa acaaggtggt tactggtgtc
   104821 tctttcagcg atgctttggt tgaaaaagcg gtcacagccg gagcggacgc tattcttgtt
   104881 caccacggaa tcttcggaaa ggaattcttc agactttcag ggtatctaaa gaaaagggtt
   104941 gagagagtat tgagtttggg catcacactg ttcggatatc acctgccgct cgacgccaat
   105001 gagccatatg gcaacaacta ttcaattgct gaagagatag gactcgtcaa cctcgagaga
   105061 tatgatgtag gctttatagg aactcatccg gaaaacattc tcgtgtctga gttgaggaat
   105121 tccttatgca ggattttccc acgtcaagag gttggcctgt tcaagaactc cgatttcgct
   105181 ggaagagtat gcataatatc aggcggttct tcatcaaatc tgagcgagct tgaaggcaca
   105241 gcagacacct tcatttcagg ggaagttggt gaaccaaccc gggagattgc aaaagagaca
   105301 ggtatcaact tcttctgggt agggcactac gcaagcgaaa gattcggtgt gattaatctc
   105361 ggagagctgc ttagagagag attctcgctt gaagtggaat tcgtcgagat ttacaacgaa
   105421 gtctgatcgt ttcttccaaa ctcgtccttc aaaataccca taatgatctc gtcatggaat
   105481 gatccatccc ggaaaatttc ctgtctcaag gttccctcga ttctgaatcc acatttttga
   105541 tatgacctga ttgctctcct gttgaatgaa tagacattta attttgcttt attgatgttc
   105601 atctgttcaa agacgaatct tagaaggatt ctcattgcat cggttccata accttcattc
   105661 cagaacttct ttgcgatgaa tattccaacc gttactacac tattcttcca gtcgacttcg
   105721 ttgatgccac accctccgat atattcattg tcttctatct ttgctatggc gaagctataa
   105781 gtgtctttag aagagctttg agaatcatac cacttttcct cctcttccag tctcaacgga
   105841 aaaggaatcc ctggcaccag cattcttttg acttcatagt cgtttatata ttcatgagca
   105901 agtacaatgt cctccttccg gtattcccta agatagacct ttcgcccctt caacactctc
   105961 atcaccccca tttgagatag tataacaaaa agggcggcgc ctggtagcgg gcacccccct
   106021 gcaaattgcc gctcttttta ttcctcagga gcctttgtca ttacgaaaat gccgttaaga
   106081 agtagtccga ctagagctgc cagacctatg ccttcgaacc ttacagggcc tattccaatc
   106141 gccgcgcctc caattcccac aaccagcatt acagagataa ttattagatt cttgccatca
   106201 acctttactc tgttctcaat aagtgttttc atccctattg cagcaatcat accgaacaag
   106261 agtatttcga ttccacccat aactgccgta ggaatcgact gtataagagc gccgactttg
   106321 ggaacaaatg acagaatgat tgcgaataca gcagcaatcc tcatgactac gggattgaaa
   106381 actttggtga tagcaaggac ccccgtgttt tcactatagg ttgtattcgc cgggcctccg
   106441 aaaaaaccgg cgactgccgt cgcaattcca tccccggcaa gtgttctgtg aatgcctgga
   106501 tcttcatagt acttcttccc gacgacagct gatactgcga aaatatccgc gaagtgttca
   106561 atcgtgggtg ctagtgcaac agggacaagc actgtcaaag agtagaatga gaacttcgga
   106621 agaacaaatt gaggcaggcc gacccagctc gcctgactga ctgcatcgaa tgttacattg
   106681 cctgttatag cagccacgat gtaccccacc acaatgccac acataacagg gattaatctc
   106741 gtaaaaccct ttgcgtaaag cctgacaaaa acggccgttc ccagagatac gattgcaatc
   106801 caccagttcg aacttgccat gcttatcgca gtcggagcga gatttagacc gatgactgca
   106861 atgatcgttc ccgcaacggc gggtggaaag atcttttcaa acatgctaac tccaagtctc
   106921 ttgattaaca tcgcgattgc tacctgaaca aggccagcaa tcagtatacc acctgtggca
   106981 tacgctatca tgtccggagt tatggagaac ccggctgcaa tagcttcagg aacagattca
   107041 taaatttccg gtacatttgc atgatagaga attactgcat tgattggcgc aatgtaggca
   107101 aagcttgaac ctagaaagac cggcaccttc cacttcgtaa gcagatggaa aagaagtgtg
   107161 cctatacctg ccgtgaataa ggcaacattc acaggaacgc ctgtcagata aggaacaaga
   107221 acagttgctc cgaacattgt gaatgcgtgt tgcaggccga gtactaaaag tcctcctcta
   107281 cccagattct tgcttctgtc ttcctgctcc cttacctcct ggtaagctga attctcactg
   107341 ctcacaaaat ccctcctcaa gatttaaatt tgtccacgaa gcaaaaaaaa acctcacgat
   107401 atctcgtgag gtctaactga agaatctatg gcttttgcga ttttgatcat caccctttga
   107461 actgtgcgga aagaagacaa agggctttta cggagggaaa aaagagctct gtgtcgatta
   107521 aaaagacaga tcttccactc ttcatacaaa gccaggcaca tatagctcac tctcccaaaa
   107581 attcatccaa agatcattct atcacaaaga acacagattt ctcctcagat gcccaattgt
   107641 ttcattcagt aaatcttttc gtatagatta ctcagccagt cgctgagcgg aaaaaacgga
   107701 gaaaaaacat aacacgactt taatcaacgg aatcgcctta taaagaacag aaaatcgaaa
   107761 ttaagcaata tcactgacga ccactcgaat cttgaaacat ctcgtcagtt atatggaacc
   107821 gtacaacttc actagatcat gaagatgtct tcgttcctcc tcgataatat ccttcagttc
   107881 cttgcagtca ggtatgaaaa aagaaagttc tgaatagaat attatgctgt ccttttctac
   107941 ttccatcgca ctgctgattg ctttgttaat gttgtttgga acatcaatcg attcaattcg
   108001 tggaaatata gatatctctg cataggactt caaataacca acattatctt cccaatcttg
   108061 agcagtcggt gaattttcag cttctttcaa catctctctg aatattgccg catgctccct
   108121 ttcctccgtg gcaagtttca caaacaacga tttcacttcg cccgaagttt tctcactgag
   108181 tttctgataa taactataac cgacactctc aattctcaga gctatgtcta acatgtcact
   108241 tacagtgatc atatgcaacc tccttgtcaa atctgatttc gattattcga gactctcaaa
   108301 acaaaaaaga aagagatcaa tgacctctcc ccactttcat agattaccga acccctatta
   108361 tttgatttcg tctttctccg aaacaatctt aatggtgatc cccgttctca tctcttcacc
   108421 aatctgaatc tccacaaatc cagggaccat gtacactttc gtaccctgtt ctctgaggaa
   108481 tctagaagcg atggcaactg ccttcacagc ttgattaaca gcaccagcgc caatggcctg
   108541 aagctctact tgctcgttct ttgagataac tccggctatg gcacctgcta ccttgttcgg
   108601 attcgatttc gaactgacct tcaggatttc catcgagatt tcctcctttt gcaaataatt
   108661 ccgctgtaat tctaccacag aggccgctta attagcaaaa gttttaacac atcgtttgca
   108721 aaaagaaggg aggatcgtta ctgattgagg tctacagatc cattatttca gacttgaccc
   108781 ttgatattgc gtcaagaact tcttgaggag taatcaactc accactgacc ttactcacat
   108841 gctctatctt tgcgcctcgc ttgttcaatc tagaaacttc atagagaatt tgccctagat
   108901 tcatctcagc aacaatcacg gcgctcacct tttttagcag ctgtcttaac ctagtactgg
   108961 gaaacggcca aatcgtaacc ggtttgaaaa ggcccacatt gatcttgtcc tctcttgcca
   109021 ttttcacagc cttaatggcg ctcctggcaa cggaaccata tgcgaggacc agtatttctg
   109081 catcttcagt catatattca tcatatatta ctatctcatc gctgtgcaat cttatcttgt
   109141 tgtcaagcct tctcaaaagc ctcgcggaat cggaaggaga accaaccggg aaacccgatt
   109201 catcgtggac cagacccgag acatgaaacc tggacttacc catcttgacg agtggagttg
   109261 ccatcatctg atcgtcacct tcaaatggat ggaagatatc ttcttcagca aattcagcct
   109321 caccaactct ttgcacttcc attatctccg agtcgtacgg gaggtaaaca ctctcgcgca
   109381 tatgaccaag gatctcgtcc attagaaata tcacaggtgt tctatacagt tcagcataat
   109441 tgaacgcagt tataatgtac ttatagactt cttcaacatt tgaagggtag attgcaatga
   109501 tctggtgatc cccatgtgtt ccccatcttg cctgcataat gtccccctgc gccggtttcg
   109561 ttggcatacc ggtcgagggg cctcctcgct gaacattcac tattactgcc ggagcctcag
   109621 tcatcgccgc ataaccaatc gcttcctgca ttagcgaaaa accaggtcca ctcgttgcag
   109681 tcatagattt ttcgcctgca agagaggcac cgattatagc agcagcactt ccaatctcat
   109741 cttccatctg aataaaagtt ccaccacact tcgggagctc cctcgccatg gtttcggcaa
   109801 tctcagtagc cggagtaatt ggataaccgg cgaagaacct gcacccggcc ttgattgctc
   109861 caagcgcaca tgcctcgtca ccctgaagaa gaacgaactc acgcatcctc tttcaccgcc
   109921 tccacttccc acacatcaat cgcaaagtca gggcatgcgt tctcgcacat aagacaacct
   109981 atgcatgtct tttgatcaac cacttttggc ctgcctagct cccctttagt aatcgttttc
   110041 gtcgggcaga tccagtagca tattccacaa ctcttgcaca gagccagatt aatgtttact
   110101 ttgtactgct gcttttttac agccattaca ccctcccact ccaggttatc atattattga
   110161 aagaaatact ccggctgcaa ctgcagaacc aattacacct gctatgttcg gtcccattgc
   110221 atgcattaag acaaaattag tcggatcttc ctccgaggca attttctggg caactcttgc
   110281 ggcagtagga accgccgaaa ccccggccgc acctattagc gggtttactt tgtttgcgga
   110341 gaacagattc atcaactttg caaatagaat tcccgaagcc gttgcaacaa caaaggcggc
   110401 gcagcccaat gccaaaacca ttatactctc aatcgtgaga aatatcttac cgtccgcttt
   110461 tgcgccaacg gagacgcaga gcaaaataat cacggtgtct gaaatatatt ttccagaagc
   110521 ttcaacgagc cgctttgtct tcccattctc tctcagaaga ttgcccaaca taagcattcc
   110581 cactagagga agcgcttgag gaactatcaa cgagattgca atcgtcgcaa ctactggaaa
   110641 aacaatcctt tccagcttcg agactcttct cagcttcttg cccattgtta ttgctctctc
   110701 cttgtgagta gttagaagct tcataatggg aggctgaaga accggaatca gagcaatata
   110761 ggagtaagag gctatcgcaa tgattggtag gaactgagac ttgagaatac ttgcaacgta
   110821 tattgacgtc ggcccatctg ctccgccaat tatcgatatg gcagctgaat ctgaaaggga
   110881 gaaacccatc aaattagccg cgaggaatgt tataaaaatc ccaatttgag cggctgcccc
   110941 taaaaaaagc gtcttgggat ttgcaagaac aaaagaaaaa tcagtcactg ctcctatccc
   111001 cagaaatatt aggggtggat aaattcccaa catcaatccc tgttgtaaga tccacattat
   111061 acccgtacca ggagtgaaga cacccgtagc ctcgggcgga atattggcga taaccatacc
   111121 aaacgctatg ggtacaagaa gaagtggttc agcatctttt gcaaccgcta gataaaccag
   111181 gaggcctgca attgcaaaca taaccaagtt ttcccagctt accgtggcta atcctgtctg
   111241 tgccatatat cccgagatca acgttgacat gaaaggctta cctccaaaag aataatggcc
   111301 gcaccgacag gcgcggccca aaatgaagac tatctattct ttactactgc ttgagctgcg
   111361 gacaatctcg ctactggaac cctgtagggc gaacaactta catagttcag acccacgatg
   111421 tgacaaaact caactgacga aggttcgccg ccgtgttcac cacatattcc taccttcaga
   111481 tctggcctgc tggagcggcc cttttcagtt cccattttca cgagctggcc aacacccact
   111541 ctatcaagct tctggaatgg atcgttttcc agaatacctt tttccatata atgtccaacg
   111601 aactttccat agtcatctct actgaaaccg taagtcatct gcgtaagatc attcgtccca
   111661 aagctgaaga attcagcttc ttttgcaatt tcatcggcag taagagcagc tcttggaact
   111721 tctatcatcg tgcccacttt gtaatctagc ttcaatccag attttgaaat gagttcgtct
   111781 gccgctttta ctatcaccga ttttacaaac ttaagctctt ccagttcaac gattagcgga
   111841 atcatgatct ctggaaccac tgactttcct tcctcaacca gctttagagc agctccaatt
   111901 atcgctttcg tttgcatctc ggcaatttcg gggtatacaa ctgcaagtct gcaacccctg
   111961 aaccccatca tgggattgaa ctcatgaaga tcttcaactg ttgtcttcaa ttcctcatag
   112021 gtcattccca tttcctcagc cagttcttta acatcttcgt cttctttggg aaggaattcg
   112081 tgaagtggag gatccaggag cctaatagta acgggcagac cttccattgc tttgaatatt
   112141 ccgtagaaat cctcttcttg cattggaaga agcttattga gagctttttc tctctgctct
   112201 ctggtactgg aagtaatcat ctgacgaacc gcaaaaatcc tgtcttcctg gaaaaacata
   112261 tgttccgttc tggtgagacc aattccttca gccccgaact ccctggcaat cttggcatct
   112321 ctaggagtat cggcattcgt tcttataccg agcttcctga tttcatcggc ccatccaagc
   112381 agtttcgcaa gcggtccttc gagaccctga ggtttaactg aatcgagttt acccatgaat
   112441 actttgcctg aagtcccgtc aatgctaatc cattcgccct catttatggt ttttccattt
   112501 actctcattg tccttgtctt ctgatctacc tctacggctt cgcaacctac tatacaagtt
   112561 ttacccatac ctctcgcaac caccgctgca tgtgaggtct tacctcccgt agaagtcaga
   112621 attccctgag cagcagccat tccggcaata tcctcgggag aagtctcagg cctgacaagg
   112681 ataaccgcct tgccttcttc tctaaccatc ttttcagccg tatctggatc aaaagcaacc
   112741 gcacccgaag cggctccggg agatgcagca attcccttag cgagaaactc tgcactctcc
   112801 atagtttcct tcgtaaaata cgggtgcaac agagtttcaa tgtgttcggg cgttactctc
   112861 ataaccgcct cttcctttga aattttctct tcttctgcaa ggtcaacagc gatcttcaca
   112921 cttgcataag gagtcctttt cccgtttctc gtctggagaa gatacagggt cccatgctca
   112981 acagtaaatt caatatcctg catatcccgg taatggttct caagtttctc aaaaatctca
   113041 agaagctgtt tgtagacagc ggggtttatg tcgttaagct tcgtaagggg gaagggtgtt
   113101 cttgtcccag caacaacgtc ctcgccctgg gcattcggga gatattcacc gtagaactgt
   113161 ttttcacccg tattggggtc tcttgtaaac gcgactcctg ttccactgtc atcgccggtg
   113221 ttcccaaaaa ccatcgcaac gacattgaca gctgtaccaa gaagttcgcc ctcctttatt
   113281 ccattcagcg cgcgatactt ctttgccctt tcattcatcc agcttccgaa aactgcgttt
   113341 attgccatcc acaactgctc ccaaggatcc tgtgggaact cttttccagc cctgctatag
   113401 acctcaagat atctcttcac gactttcttc atatcatctg catcgagttc agtatcaagt
   113461 ttgacaccct tttctttctt tatactcgaa agcgcatctt cgaattctga atgctcgatc
   113521 ttgagaacga catccccaaa catctgaaga aatctcctgt aggcgtcgta acagaacctg
   113581 ggattacctg attctttgat cattccattt accgtctcgt cattgagacc aagatttaaa
   113641 atagtatcca tcataccagg cattgaaatt gctgctcctg atctcacaga aacgagcaat
   113701 ggtctctcac tagaaccaaa tccctttccg gtttcttctt ccaaaagtct tacagcctct
   113761 ttcacttcat tctcaaggct ttccggatat ttattaccat gctcgtagta gtacttgcag
   113821 acttctgcag agatagtgaa ccctgggggg acgggaattc ctattttaac catttcagcc
   113881 agattggctc cctttccacc gagaaggttt ttcatctttg catcgccttc ggcctttttc
   113941 tttccaaaat agtacacaaa tttctctgac actttctttc ctcccttcct gaaagcatat
   114001 tcgactgatt atatcatagc taaacatacg aggtgtttag ctatcgctct aatgttgaac
   114061 gtctcttgta ccatatcttt ctctttccgc agggcagaaa atcattgctt tgattctttc
   114121 agcaccctcc taaagaacag agccgcctta tggcggctct gttctcatcg aaaaacaggt
   114181 caatctataa ggcccaatcc ttttaggaat ttttcggcaa cttcatccgg ctcaaatcct
   114241 ccagcgtcta ccagataatt gagccttatc attattggtt cagtaagata gacagagatt
   114301 ggcctcagga tctcttcaat ttcgggattg ttatctaata tttccttccg tactgtaaca
   114361 gctaggttgt atgatgggaa gaaactcttt gtatcttcaa gcacaagaag attgtacttc
   114421 tccagttttc catcagttga aaaaaccatt gcaacatcga tttggttctt gtctagtgcc
   114481 tcatatgtca gcccgatttc cattgttttc acttgtctct tctccacggc aaaaccatat
   114541 gcctcactca taggccagaa tccatcgtct ctttcaagga actcgtatac gattccaaag
   114601 agaagatctt gattttcgtg agtcagctca gccagatctt cgagtgttgt gagtccatgc
   114661 tcagcagcga aatctcttct gacggcaagg gcataagtgt tgttgaaatt tgccatgtcg
   114721 agccatacaa tcccattttc gagatcttcg gcctttactg cctcgtaaag ttcatgagga
   114781 tctctaatca tcttttcgtg acccaaatat gttggccagg ccgtacctgt gtagtcagca
   114841 taaaggtcaa tctgcccggt ttcgagtgca cttcttgcaa caaatgagct catcccaaaa
   114901 ttctcggtca ctctgaaccc ggcatcttcc agaagatgcg aaatcatgct actgacaatg
   114961 tactgctctg taaagttctt tgctccgaca ttcaatcttg tttggccgaa tgacaaaacc
   115021 gttaccatta atactaccag cgtaaaaaga aataatctct tcaaaactaa acacctccat
   115081 gtttcctatg ttctgaggcc tttcgaagtc agagccttct ccgaaaaacc caggattatg
   115141 tctaccgcta tcgccaaaat cgttacagga atggctcctg caattatcat tggacccctt
   115201 gtcatacgaa ggccagtgaa gataatctct cccagaccac ccgctccaat tgatgcggca
   115261 atcgcagcag taccaatatt tatcgtcgcc gaactcctga ttccagatag cattgtcgga
   115321 accgttacag gcatttctac cttccacaat atctgcctgt tagtcattcc cattccctta
   115381 gccgcttctt tcatcatcat cgaaacattc atcaagccgg atgcagtatt gaagaccacc
   115441 ggtacaagac tgtaaaggaa gagagcaact acggctggcg cggctccaat tcccatgaac
   115501 aggaaaacaa gagcgatcac tgcgatactg ggaacggcct gagttgctcc ggtgatcgac
   115561 agaaccaatt tgcccgcttt tctgtgccct ggcctggtaa caaaaatcgc aattgcaacc
   115621 cctgccacta ctgccatgct ccaggaagta aggaagatga aaatgtgctc aagaagcctt
   115681 tccataatgt agccagaatt ctcgaacata tattctaaga cttccatggt ctcactcctc
   115741 atcagacgac tcataagagc ctacgaggtt tacaagatcc ttgaacctca gggttccaag
   115801 tagcctctct ttatcgtcga caaccggcag tgaggaaagc ccggtattca tgatcaatga
   115861 catcgcctcc atcaaattcg aaagaggttg tatggtctcg atggatctca agtacttctt
   115921 taggggctct tcatcagacg ccttgtttag ctgtttcaac ataacatacc ccttgagtct
   115981 tttgcttcca tcaacgacat aggcgaattt tcttccctcg tcaatgagaa aatcctttgc
   116041 tttttttacc ttgcaatcac ctgtgtcttc gatccacttt ccgccaggaa cgtaagtctc
   116101 ccttactcgc acgagttcaa gacccttcaa cgctctatct gcgccaagaa gatcttcaac
   116161 aaactcatta gccggttcct gaaccaccgt cattgtatcg gcatattgca ccagttttcc
   116221 atcgtctaga attgctatct tgtcacccag cttgattgct tccctgatat cgtgagtcac
   116281 gaagataatt gtcttctcta tcttcgactg aatttcgaga aacccgtcct gaagcttttc
   116341 acggttaatc ggatctattg ctccgaaagg ttcgtccata agaactattt ttgggtcgga
   116401 agctagacct cttgctactc ctactctttg acgttgtcct ccggaaagtt gggctgggta
   116461 tttctctgca tagtcggaag gttcgaggtt tacaaggtcc agcaattcaa aaacatgttt
   116521 cttgattctt gtttcatccc acttcatcaa tcttggagtg acagcaatat tatcgaaaac
   116581 cgtcatgtga ggaaaaagac caatctcttg gattacatat ccgatgcttc ttctcagtct
   116641 aatcggatcg atttcatcaa gcgatgtccc gtcaacgaca atttctccat cggttttctc
   116701 aattaaccta tttatcatct tcaaagtagt tgtcttccca cagccagagg gacctattag
   116761 tattgtcaac tccttgtcct caaaggtaat actgaggttg tcgactgctt tgaatccttc
   116821 ttcgtattcc ttaacgacat ttttgaattc tatagacatc tatgttccac ctcacttttc
   116881 cctctcgatt ttcagtcctc tgggagtgat gatctcttct agagcgagta aaccatagtt
   116941 tgttcctatt cccagtatgg aaactaccag agctcccaga agaaccatat tgaaatttga
   117001 cctgcctagc ccagagaaga tgaagtatcc aagtcctctt gcgccgataa ggtacgcgat
   117061 agtggctaca cttacaccca taacagcagc gtttcttacg cctgccatta taatcggtat
   117121 agtgagcgga agtcttaccc tgaagaggat ctgccagttg gtcatcccca ttcctctggc
   117181 tgcttcaatc attctgggat ccactgaatt cacagcaaca accgtgttcc ttattacggg
   117241 aagaaatgag taaattacta aagcaatcac agctggcgta acacctattc cagccttaag
   117301 tggcgctaag agaactacca tataaccaaa cagcgcaaga ctgggaatag tcataaggat
   117361 tcccgctcca tatagggcaa cagaagaagc ccgtttgctc ctcgaaacca caatcccgat
   117421 gggaacacca atcccaatcg cgatcgggat agctatagca attatcttta tgtgattaag
   117481 taactcaata agaaccaaat cgtaattcct tcccagatac tcaaagaaac ccataaatct
   117541 ctccttccat aatcggccaa aaaactggag tacggtcaag acccttcctt atgatcgagt
   117601 attaagtttc tgactaaaga ttattctttg ctcgagtaga tagagatggt tgtgtcaaga
   117661 tcctaaccaa aattattaaa acacaagact accataacta atatactata tcacatgctg
   117721 gaaagctatt tctcacaacg caaggttggt tcgtgaagat tgatttaact taagaacttg
   117781 atttgaagac aattataatt t
//
LOCUS       DAOQYI010000004        87700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_250_length_87700_cov_76.276274, whole genome shotgun sequence.
ACCESSION   DAOQYI010000004 DAOQYI010000000
VERSION     DAOQYI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 87700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 87700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87700
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_87700_cov_76.276274"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKL93_02780"
     CDS             complement(<1..582)
                     /locus_tag="PKL93_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70218.1"
                     /translation="MKRAKLFVMLGALIIVAFAISSCFPVKGSILLLEQKIGWEPGKP
                     VVIEAAALLFTPDPSSNYQWLVEVKPQYGSWTSRNDLVKVIPSTKNSKAVVYLPVNEN
                     FEIKITAKISGTSVSGEHGNYSSEIVKHVSEIPDVLVEVFEKSLGFNMPDLWFEDYLN
                     GNRSNSTRTPVYYKLNKALHPTAYYNRIGWEVYP"
     gene            complement(579..3173)
                     /locus_tag="PKL93_02785"
     CDS             complement(579..3173)
                     /locus_tag="PKL93_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ70219.1"
                     /translation="MKVITTHRNPDFDAFASAVAAQKIYPDHMIVFGGQLVPALKRFL
                     SSKSLLLSFYYVNEIRISNLSSLIIVDTSDLKRIPASLQQSIQRNTDVKFYDHHRTSL
                     SEAQKGIFKDLGACTTLMCNQLRKKKKEISRNEATLFATAIYRETGNFTHASTRAQDL
                     STAAWLIDLGARPDDLGIYSGYKLSASQQRLIESLILGLRRTSIRGVEINIASARRDF
                     LPGGLSLVVEKLWSFLGVENLVVLLETNNRVYYTLRSKYMNLDTAELDGVQRNSGGSY
                     TLGYFENCTSAEAEEKIIKNFENNIERLIKVRDIMSSPVRTALAEMKVEDVFKIMQRT
                     GHHTLPVVDQEKLVGIAKYRNTEKAIRHGLAARRIIEVMDRFFVTASVDDSVQAVTDK
                     MIENDTTAILILDGGILSGIVTRTDLLKSTFGRRRIIDPGEKIGDQNYAKFPVEDLIE
                     ERVEKRIVTMLRFLGAVGSELNMPTYIVGGFVRDLLLNKKNLDLDIVVEGNANLFAET
                     FKKYFNIKIVEHREFLASSMFFNDGLRIDVATARTEYYKKPAILPEVEMSTIKKDLYR
                     RDFSINAMAIKLNQEDFGILIDFFGSRKDLSKGIIRALHPLSFIEDPTRILRAVRFEQ
                     RFGFEIEVRTAELLKQCAAEGYLDRVTGQRLRDELSKAIEEPLPLKALRRLASLGVME
                     KLFPETEFDRETDIRLQRYFLRRERNALLIGEDRMFYTLLMIILRKSDSKSVEWCIAR
                     YGLPKNYLERLNDAIATAGIIVNEKPETPSQFYRSLKTNKPEILNFVNSYLDEQEDLL
                     FLAYLKRLNDTKLSINGNILKARYGMREGPEIRDVLDELFCARLDGLTKDKEDEFVRE
                     YLKGRGWK"
     gene            complement(3148..4494)
                     /locus_tag="PKL93_02790"
     CDS             complement(3148..4494)
                     /locus_tag="PKL93_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNQ70220.1"
                     /translation="MKRKVLVVDDDLAFNGLLGAALEEEGYEVVQVYNLEQTLKQISE
                     EVFDCLILDVRLPDGSGLDIISEAALSSPVIVVSAHGDINTAIDAVKKGAFNFLEKPF
                     DLNHALLEVKRAIEFSEVSRERDSLADLVTTEPSVEIIGKSKNILELKQMIAAIAPKK
                     VTVLLEGESGTGKEVVARSIHQKSGRGKFVPINCGAIPENLFESELFGYEKGSFTGAM
                     NSKPGLIEESHRGTLFLDEISEMPLPMQVKLLRVLETSSSQRLGGTTSRKLDLRVIAA
                     TNRDLEVEVSKGNFRSDLYYRLNVVKIKIDPLREKREDIPILIHHFLPRLCEELGMKK
                     PPTIPEDFMNKMKAYDWPGNIRELRNRLLSILAVNGSFEGVSSFAFPEVEQADDQDFR
                     FREVSLDELERRYISWLIDRQNGNKTKAASILQISKSTLYEKLKRWSNLEGNNDTS"
     gene            complement(4469..5779)
                     /locus_tag="PKL93_02795"
     CDS             complement(4469..5779)
                     /locus_tag="PKL93_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ70221.1"
                     /translation="MSKLDGGLFKPGYHYIVGGVVLGTGLIYSFIEGFSLDRVVLIAL
                     LSLAALASVYFALVRQLERLVRLVQTGERKSIGELEGQFNQIYGFYRSLSFQIEEERN
                     YYERLYRDYSELLNTLEISVASVDENGNFDLVNDAFTKSFSYGSQQGRYLRIDRFARR
                     TGLIFPLQEGQYEVYSKKLGKRYLVSVVHKQKSGYLLTLTDMTSQWKTKQLLEKTRRY
                     AVDAQTVADLAHGLKQPLANVRLALDLYRRTGKPEYIDTLEKELNAFQGRVGGILQIF
                     RYGEEFDVVDLSEQVMKVATFMSSIFQERKIDFRIISLEKGLVLAQRGRLENVIKNLF
                     MNAVEAGLPSETSIIAKVRASKEYLTLIVADTGKGIDPSNLQKVFKPFFSTKTEGSGL
                     GLYLVHNFCEENDVRLKMRSVKGKGTVFVLTFRRQSDEAQSPCG"
     gene            complement(5766..6548)
                     /locus_tag="PKL93_02800"
     CDS             complement(5766..6548)
                     /locus_tag="PKL93_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNQ70222.1"
                     /translation="MIAFLTDWGSNSYYVGVTKVVMREINRMVEIIDICHDIRPYSIR
                     HGAYVLQRAIKDLPADAVFLAVVDPGVGTSRKPIMMVLKDGRRLVGPDNGLFTLAAEE
                     YGIHEIRELENREYHRGNSRSFHGRDIFAPVAAHVDAGVEPAKLGSRLMSFEYLKYKK
                     PAREGNVLSGEVAFYDNFGNLETNVPEALANDFEEGEILEIGKGRKPFKATFGNTYYD
                     VNPGNLLAHFDSSGFLEITVNKGSARELLAIEEGEPITIVKT"
     gene            complement(6550..6975)
                     /locus_tag="PKL93_02805"
     CDS             complement(6550..6975)
                     /locus_tag="PKL93_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HNQ70223.1"
                     /translation="MLSYNIYIMDYNKGDYIVLLFLREAAEVSSSKKKWHLESGYYLY
                     VGSAMNSLTERVKRHLLEEKKKHWHIDFLREKAEVLAVLLLPAKTSNEEELSNLISNY
                     CIAVPGFGASDCKTDSNLYRVGFGCIEKVFSSIVGKWRD"
     gene            7034..8104
                     /locus_tag="PKL93_02810"
     CDS             7034..8104
                     /locus_tag="PKL93_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HNQ70224.1"
                     /translation="MISLAKVVVNTVSIGANAKRVRELAEVNGVEIAAVTKVVCGDPT
                     IASILLNSGVKEIGESRLENISRIMNAGIDSDFLLLRLPERSKFREVLDNVDTVLIGD
                     LDSLSKLYELSSETGQEVNFIYMIDTGDLREGVMYYEAESVLEKAFEIAGENLEGIGT
                     NLGCFGGVIATPKKFEILLSLGEFLRKKTGYSLRRYSAGNTASLPLLEKGIVPKGINH
                     FRLGESILCGTDVTNNREVPGTLQDTFTLYGEVIEIAEKPSVPIGEIGRDAFGRIPHF
                     EDKGKRIKAILDLGEQDVLPSGLTPLVGGCEVLHASSDHLIVDVTESEKSFSVGDSIG
                     FRMSYGALLRVMTSPYIRKVYE"
     gene            8101..8916
                     /locus_tag="PKL93_02815"
     CDS             8101..8916
                     /locus_tag="PKL93_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ70225.1"
                     /translation="MIRRLQKCDGERVREIVKTIWEGDDYIPHVFEKWVEDPSCHFMG
                     LWKDGTLVGIDNLRLLSDKVGWMEGMRIDPALQGKGFGKELGKQTLAIAKRIGLERLY
                     FATYFDNTASIRMNEAFGFNRIGVFTNLESEIGDISDSSFAFNVDSEIPEILDHVSED
                     WAFLPKEIPDKARFLIQPTRIHDGDNWAVLSKNSKSESCVDINYISIVEERKARSFVH
                     GLKCYAQSKGFERVHTMVREDLDLEPFLYNGFKPFERVRDVFLYFADSSVLEI"
     gene            complement(9647..10459)
                     /locus_tag="PKL93_02820"
     CDS             complement(9647..10459)
                     /locus_tag="PKL93_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70226.1"
                     /translation="MIKRILIYALLTVFALFFLMPIYVLLATSLKPLKEVGLERMWFP
                     PNDLSFDGFAKAFSRLAPNLRNSFLLVIPATLLSAIVGSINGYVLSKLKFRGSDLLFA
                     LVLFGMFIPYQSVLFPLIRFLQDIGLYGSIWGLVLVHVVYGLPITTLIFRNYYSEVPT
                     ELIEAANIDGAGIFKTYLKVLFPISLPGFVVVVIWQFTNIWNEFLFAVTVTSDPTKQP
                     ITVALVNLAGSQVVEWNVQMAGALLAALPTLIVYILLGKYFLRGLLAGSVKG"
     gene            complement(10456..11394)
                     /locus_tag="PKL93_02825"
     CDS             complement(10456..11394)
                     /locus_tag="PKL93_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70227.1"
                     /translation="MFHIKRKTKRGIISFLILSPTFAAIGVFVYFFIGWTGRTSVSNW
                     NSFARLLKGEFEFVGLRNYFRLFEDPRFQTDLWNTLYFTLFFILGCLLLGIVLAVLID
                     RNLKGSGIFRNIYLFPMALSFVVTGAVWRWIFAPGILPSNPQGMNLLFSLVGLDFLQW
                     RWFTSTESFLNFNFALIPVIIAAVWQMSGYTMAMYLAGLRGISQDLVEAAEVDGATGW
                     QIFWKIKFPILRPITLSAMIILGHISLKIFDLVYAMTGSGPNNVTDVPAIYMFETTFR
                     ANKYATGSAIAIIMLLMVAVVIIPYLASAFRKEKRI"
     gene            complement(11439..12671)
                     /locus_tag="PKL93_02830"
     CDS             complement(11439..12671)
                     /locus_tag="PKL93_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70228.1"
                     /translation="MRKSLLAVLLLSVSLLFASGSVEIFSWWTGGGEEEGLAAIYEVF
                     KAKYPDVEIINATVAGGAGTNAKAVLKTRMLGGNPPDSFQVHGGMELIDTYVITGMME
                     PLTDILESWGILDKFPKDIMTICSYDGEVYSIPVNVHRGNVVFINNEILEKVGIDEVP
                     SDGPGFLEVCEKIKEAGFIPLSLGDKDKWEAGHLFETVLLSALGPDKYNGLWDGTTSF
                     EDPGIERAIVIFEELIKYVNEDHAALTWQDATRMVFDGKAAFNVMGDWAEGYLKTLGW
                     TPGVEFSWMAVPGTEGSFMVVTDTFGLPKGAPNRENALKWLEIVASVEGQDAFNPIKG
                     SIPARLDADKSLYDPYLTWSMEDFSTNALCPSIAHGSAAPEGFITELNDAVNIFITTK
                     DRAAFLSAIKSAAEDYIY"
     gene            12982..14391
                     /gene="aglA"
                     /locus_tag="PKL93_02835"
     CDS             12982..14391
                     /gene="aglA"
                     /locus_tag="PKL93_02835"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032450 - maltose alpha-glucosidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase AglA"
                     /protein_id="HNQ70229.1"
                     /translation="MNISIIGAGSAVFSLRLVSDLCKTEGLYGSKVTLMDINQDRLDA
                     VYILASRFAEEMNANLEFSKTTVLEEAIKGRDFVVNSALIGGHAFLDRVRAIGEKHGY
                     FRGIDSQEFNMVSDYYTLTNWNQLSFFLKIAKLMERQAPDAWLLQAANPVFEGTTLIR
                     RHSSIKMVGFCHGHYDVREVAETLGIPIEEVDWQVAGVNHGIWLNRFKRNGEDLYALI
                     QKYAANYDPRRFKPINPFNVQMSPATIDMYRFYGLVPIGDTVRNSGWKHHYNQETLVK
                     WYGAPWGSPDSEPGWQWYKEQLGQVTGATVALAKAIEQFPEASLEKLIVEGSRGLSGD
                     FAKQAAQLYNKNSLSGEQHIPFIDAIINGSSARFVLNTLNEGAIPGIEDNVAVEVPAL
                     VDREGIHTEAIDPPIPERIVKWYLKPRVLRMEWALEAFIERDSDLIVEILLKDPRTKS
                     YEQAKAVVREIFETEGLNE"
     gene            14516..15103
                     /locus_tag="PKL93_02840"
     CDS             14516..15103
                     /locus_tag="PKL93_02840"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNQ70230.1"
                     /translation="MVELNEVFRCPWAEVDKLYIEYHDTEWGVPLHDDNIWFEFLILE
                     GAQAGLSWHTVLKKREEYRKAFSGFDPRAVSKYGENEITKLVENPGIIRNRKKIHSAI
                     NNAKRFIQIQEEYGTFDSYVWSFVNNKPIINTWESLSEIPSVTEKSVEISRSLRDKGF
                     TFVGPKIVYALMEATGMVNDHLVGCFRYSEVLRCN"
     gene            15176..>16011
                     /locus_tag="PKL93_02845"
     CDS             15176..>16011
                     /locus_tag="PKL93_02845"
                     /inference="COORDINATES: protein motif:HMM:NF040149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70231.1"
                     /translation="MSWLNTEEIFALIDLIDSSNDSYEERFKRVLATPLASMFKQFHV
                     RDSEARECLVAAFSRREDLSGLSKDCTELIAYMDKIRERKLELRAFFDLINENEAGIR
                     SKAISKAQEYLPKGVTFDGLKVFFIPMPYNANADHRGVYFDPLFALDMGLEGIEEVMA
                     HEAHHVARNSITKERLEFGDSPLDQLVYRFVSIECEGIANMVSDASRIPSMKRIALQR
                     TKMMGEFEKHLELLQEVFLNLSFKNISEDEARSIMERSWFSVGSLAPVGMRMALEIEN
                     ELG"
     assembly_gap    16012..16111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16112..17192)
                     /locus_tag="PKL93_02850"
     CDS             complement(<16112..17192)
                     /locus_tag="PKL93_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HNQ70232.1"
                     /translation="MGRKITDSVTWVGKVDWELKKFHGEDYSTHRGSSYNAYLIRDEK
                     TALIDTVWAPFAEEFVENLKKEIDLSEIDYVIANHGEIDHSGGLVALMKEIPGTPIYC
                     TANAVKSLKGQFHKDWNFVTVRTGDTLNLGSKVLTFIEAPMLHWPDSMFSYLDGDAIL
                     FSNDAFGQHYATELLYNDLVDQAELFQEAVKYYANILTPFSRLVIKKIEEVLSFNLPL
                     NIICPSHGVIWREDPAQIVKKYLEWADNYQEDQVTIVYDTMWEGTRKMAEAIAEGIAS
                     ASGSTAVKLFNAGKIDKNDIVTELFKAKAVLFGSPTVNKGILAPLSGMLHEVKGLSFK
                     SKKAAVFGTYGWSGESVDVLTKMVED"
     gene            17544..18197
                     /locus_tag="PKL93_02855"
     CDS             17544..18197
                     /locus_tag="PKL93_02855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ70233.1"
                     /translation="MSNDITYLFDLDGTLSSVSDEDFARRYFQLISLFANGKVDFEKL
                     MVSLKSALEVLFDRRDGIKSNYELFMESFVHFMGDHTVKWYEDFFDEFYENEYEELEK
                     IVSPRENVVEALKKLYGSGRKIIIATNPVFPHKAIRKRLQWVGVDEKLTNYVTTMENS
                     HYVKPSTEYYLEILENSSLDAKYCVMIGNDYKMDGACTSAGIEYVDVSSIQSIWRTT"
     gene            complement(18178..18822)
                     /locus_tag="PKL93_02860"
     CDS             complement(18178..18822)
                     /locus_tag="PKL93_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNQ70234.1"
                     /translation="MILGICGNIGAGKSSLTSLLEETLGFRGVYEAVDENPFLEDFYS
                     NMERWAFHSQLFFLIKRFDFLKRVVTEGAVILQDRTIYEDVEIFARNLKLMGYIDDRD
                     WRLYLDTYETLSEHLTAPDGIVYIKCTKNVLLKRIKKRGRGFESGVSEDYIERLNGLY
                     EEWIGRVTFCRKLIIDGDKYDFVENAQDREDVLRLISRFTAELMAGVQSKLFSR"
     gene            complement(18819..19436)
                     /locus_tag="PKL93_02865"
     CDS             complement(18819..19436)
                     /locus_tag="PKL93_02865"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNQ70235.1"
                     /translation="MMFSKASGKYISVEGVIGVGKTTLVKLLSQKYTMPVVLEVVEEN
                     PFLAHFYEDMERWAFQTQLFFLISRFDQQSNVKKAASQGQGVISDYAFMKDHLFASLT
                     LKGEQLKLYEKIFSVLKDQVLYPDLIIYLYADIDTLMKRIALRDRPFERRMDRTYISM
                     LSDTYEKYMAAFSESRVLRIDTSSIDFVRNVDDLNMVFSRIEASL"
     gene            complement(19565..20026)
                     /locus_tag="PKL93_02870"
     CDS             complement(19565..20026)
                     /locus_tag="PKL93_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNQ70236.1"
                     /translation="MYSCKIIRVLESGFFDEISQSIEISKKILESHGITYTGSTDVDP
                     VMSDLKEAIYSDSCSNDIVLVLGGTGLYKEDIGPEVVLSLVDKRATGIETAMLRNAIV
                     ADPSLCLYRVGAGTIGDSIVLSVPGYHETVHYYLESVIDYLKPFFDGLRTS"
     gene            complement(20041..22593)
                     /gene="pulA"
                     /locus_tag="PKL93_02875"
     CDS             complement(20041..22593)
                     /gene="pulA"
                     /locus_tag="PKL93_02875"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HNQ70237.1"
                     /translation="MKRVLFFLLLIFGTTIFAFTGESMGKSAEDYDAATVIIIHYHRY
                     DDNYDGWNLWVWPDKPKSMDGKSYSFDRRDEFGVVTTVKFSEKHSRLGFIVRLREWEK
                     KDITADRFVDIPDSGVAEIWVIEGEEDFLTSPDRIDLTPRVKMAFLDSLSEIYASLSA
                     PVDTKTVLPEVLLDGSPVKVQSFEKADPTDVSVTNYVKITLTEPLAPKSVSKRLSLEI
                     EGFLPGDIIVRKALDNSEFFYQGELGSIYSEKETVFRVWSPVSSSATLILFDDLYSDS
                     FIEVPMKRIEAGVWEAIVQGNLHLRAYKYRFVSYGKERETVDIYSRAVTKNSERSVVV
                     DPAKTIIEGWAQQKVPMLNKPEDAIIYEIHIGDMTSDPNTNVENKGKYLGLTETGRTG
                     TDGVTVGIDHIVELGVTHVHIMPFNDIHYVNEGEEGEYGWGYDPYLYMVPEGHYSTDP
                     YSPLSRILESKAMVMAFHEKGIRVILDTVYNHTATTGSSSPFDQTVPYYYYRTDATGA
                     YLNGTGVGNEIASERPMMRKHILDSLKMWVNEYGIDGFRFDLMGLIDKETVLAIDREL
                     HAIDKTILLYGEPWGGWGATVTFGKGDQRGTGVAVFNDNLRDAIRGSVFDEKVKGFSL
                     GSRAKERRIMRGIVGSIEYSSDIKDFADDPSETINYVSAHDNQTLWDKNSAAMPDASR
                     ELLTSAQKLSNAIVILSQGIPFLHGGVDFARTKFGNDNSYNAGVDLNKMDYSRKAEFI
                     DLFTYYKGLIELRKNHPSFRMSTAEEIRKNLKFLDAPRNIVAYVIDGKSAGDSWEEIL
                     VIFNGNLEPAEITLPDESWNLVLDRDRISEKPLERLSGKVKLEPISTYVFFK"
     gene            complement(22607..23569)
                     /locus_tag="PKL93_02880"
     CDS             complement(22607..23569)
                     /locus_tag="PKL93_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70238.1"
                     /translation="MEDNKPLRSLYLLMVIVFIALPHSEVTFANSQIMEGFYYVTPEA
                     SVSGFTGIIVGTENNYDLLIIANSHLKRYSLSGVEIPQYDSTDSNSKKCLNLIRNTIG
                     LEAIVHEVTTSKEASAYVWILLDGEAYLIQSIIVSSGLSKAVDGGEIELVPAEEFARE
                     RSLGIWAEEPEVLESGIEETSQREAIEEVVEEDSRGAARLTISKIDIFDNGEHALLTL
                     AADKGGYLEGSEVSILSRADRTTDEDVFSLDSQDPIETAEELRILITLDRSRTTSFEY
                     DFLWESYLDPRGGYLKIKDENGRERLFEYEYDRRISGFTVTPLD"
     gene            complement(23688..24032)
                     /locus_tag="PKL93_02885"
     CDS             complement(23688..24032)
                     /locus_tag="PKL93_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNQ70239.1"
                     /translation="MALAAAIYIVFTVYFYLKPVVPAIEVGSDKFLHFVGFFAGGFLF
                     VILSREEFSKAFRKTFFLFLIVGPVILEFLQVLSPNRQFDILDMVFNYLGWTVPVVIF
                     TFIVRLRTLSKG"
     gene            24182..24373
                     /gene="rpmB"
                     /locus_tag="PKL93_02890"
     CDS             24182..24373
                     /gene="rpmB"
                     /locus_tag="PKL93_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNQ70240.1"
                     /translation="MSKVCEICGKSPTTGNMVSHSNKKAKRWWKPNVHKVRAVVDGEV
                     KRIRVCAKCLKAGKVTRAI"
     gene            24416..25525
                     /gene="alr"
                     /locus_tag="PKL93_02895"
     CDS             24416..25525
                     /gene="alr"
                     /locus_tag="PKL93_02895"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNQ70241.1"
                     /translation="MNSRKTFALIDLSAYRQNLVYLSGKASPAKLMVVVKADAYGHGA
                     VELSKVAQESGVDRLAVAFLEEGIKIRQSGISLPILVLNYVEKKEIPTAKEFSLTLSL
                     SSFAQLEDIIGLNKRLPDFQLVVDTGMRRLGLEWEESIELYNSAVRKGINITGAYTHF
                     ATADEKGSGFVLEQREAFGRFLHGLGQRRNRHFETHISNSAGTIYFDNSDFSYVRAGI
                     ATYGLQPSKETDENLKPILSWVSCVSFVKRIRAGYSVSYGRTFVADREMLVATIPVGY
                     ADGYNRLLSNRGYVIISGKRCKVLGRVCMDQFVVDISHIEKPVSIGDEVVIVGTQEGE
                     SITAEEIANLCGTINYEVVCSISSRVPRVYKKGVN"
     gene            25525..26586
                     /locus_tag="PKL93_02900"
     CDS             25525..26586
                     /locus_tag="PKL93_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ70242.1"
                     /translation="MKLRDIIGNEILNQINADQDKTNRDGFPFVSDLRPGSTVLSVFK
                     VHSKRIQEARDGKKFLLLTLSDKTGAIRAIDWFNAERNDSTLEQGMVVRISGRVAVYE
                     DRIQLNLDSEGIQILQIGQYDYERFMAVTTKDIPRMYDDLLSLVGRMRDDYIRALLKD
                     IFENDKKFIEKFIVSPAASKVHHAYKGGLLEHTMSVAELCEFFATKYGDSVDGDLLVA
                     GALLHDIGKVYEYTITPNGIERTNDGELVGHIAMGIEMVSKAISKIQGFPRYLQTEIK
                     HLLLSHHGEMEWGSPVIPKTTEAIVLHMADDLDSKVAQFREIEEREFNGSTASWSNYD
                     RFLNRRVFMKNRRSGDSNS"
     gene            26597..28816
                     /gene="topA"
                     /locus_tag="PKL93_02905"
     CDS             26597..28816
                     /gene="topA"
                     /locus_tag="PKL93_02905"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNQ70243.1"
                     /translation="MPDKLVIVESPAKAKTISRYLGKNYEVTASKGHVRDLPESNIGL
                     HPETLEPTYEILKGKEKVVQELSKKSKGKQVLLASDLDREGEAIAWHISELLGLPQNE
                     KNRIVFNEITESAIKEAILHPREIDMSKVEAQVARRVLDRIVGYKISPILWRTMKRGL
                     SAGRVQSVALKFMVELEKKIVVFEPHRFFKIFAKVGEDRFSLSRIEGKKFTNKSVTTE
                     EKRDEIITELSNAILHVKNVKERTSKRNAPMPFITSTLQQSAIGELGWSASKTMKIAQ
                     QLYEGIETDQGQIAFITYMRTDSTRISNQARDKAVEIIESKFGKEYVGPIRPGKKGKK
                     IQDAHEAIRPTYPENDPEKARQLLSGDNLRLYALIWNRFIASQMASAEYSVTEVELED
                     ENNKYILSLTGERRLFDGYEKIVSRSSKSEIDRDYKIGEEIKPSMIEWEEDTTKPPSR
                     FSEASLVKELEKKGIGRPSTYATIITTLLERKYVLRQSRELRPTLLGAIVNEFLNEYF
                     PDVVDMNFTANMEEELDDVENGAKKWRDVVMGFYSGFKTDLDEIDKRIKKGELRIEFP
                     TDKECSCGGYFKVVFGRYGGYLKCESCGKNESVDMTSFTCVIDGKVMLKDIAANQKIE
                     IEIDEKCPECGSPLVIRKGRYGEFIACSAYPKCKYTRNVRIDAPCPKCGGVVEKLRSK
                     KGKNYFKCSSCGELFWNEPTNEKCEICGSYLFLKVKRGGKKVLYCEKCKKEFEIGGD"
     gene            28816..29733
                     /locus_tag="PKL93_02910"
     CDS             28816..29733
                     /locus_tag="PKL93_02910"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNQ70244.1"
                     /translation="MSKRIAVVYGGFSSERDVSVKSGSNISKSLERLGAEVIGFDLKR
                     DRIASLLSLKADLFFLALHGKFGEDGTVQGLMELAGLPYTSSDSRTSAVCFDKEITYR
                     LVKAEVDLPLWKKVISEKDLEGWKIFPCVIKPTREGSSIGVFICDDASTLINRTRLLL
                     DVYDSLLLEEYIDGREITVSIIDSREGIKVLPVLEIKPKKRFYDYEAKYTPGLTEFVV
                     PAPIERSTLESIIEKSVAVYKLLGCRDFARVDGILKGGVFYFLEVNTIPGMTDLSDLP
                     MSARAAGMTFDDVVLDIIEAADKRNRRES"
     gene            29730..30278
                     /gene="hslV"
                     /locus_tag="PKL93_02915"
     CDS             29730..30278
                     /gene="hslV"
                     /locus_tag="PKL93_02915"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNQ70245.1"
                     /translation="MKTFHGTTILVLRKKGKTVIVGDGQITIGDTVMKGTARKVRKLG
                     DGSVLAGFAGSVADAMTLFEKFEEKLRESNSNLKRAAVNLAKDWRTNKILKNLQALLL
                     VADRESILLISGNGEVIEPDEEVLAIGSGGSYALAAARALLRNSQLEAEEIAVKSMEI
                     ASEICIYTNTNFTMETLGGEKQ"
     gene            30275..31663
                     /gene="hslU"
                     /locus_tag="PKL93_02920"
     CDS             30275..31663
                     /gene="hslU"
                     /locus_tag="PKL93_02920"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNQ70246.1"
                     /translation="MNREELDELTPKRIVEELNKYIVGQDKAKRAVAVAMRNRIRRQK
                     LPEEIRRDVIPKNILMMGPTGVGKTEIARRLAELTGSPFTKVEATRFTEVGYVGKNVE
                     SIIRELVDVGVNMVKQEKMGEVEGKASYQVEERILEALVPGPQTKATGPRNIIELFQG
                     QQQPKPSQEETERIRNRREDYRDRLRSGDLEDLDIEVEVEDHSQQMIMIPGMEDMGID
                     MSGVLGGMVPKKTKRRKMRIADARKTLLPIEAEKLLDMDKVVAEAIDRVQNRGMVFID
                     EIDKITFRSGSHGPDVSREGVQRDLLPIIEGTTVTTKYGQIRTDYILFVAAGAFHTAK
                     PSDLIPEFQGRFPIRVELDALSQKEFLRILVEPKNAITKQYMALLETEGVKVTFTEDG
                     LKEIARVSHSLNEKIENIGARRLYTVVEKVLEEVSFNAPEVPQEIVVDVNYVNERIGE
                     IAADEDLSAFIL"
     gene            31663..32682
                     /gene="dprA"
                     /locus_tag="PKL93_02925"
     CDS             31663..32682
                     /gene="dprA"
                     /locus_tag="PKL93_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNQ70247.1"
                     /translation="MERVEYAAIALSGDFSPSEMEEIENSGISDIHLFRCRTDLNKVK
                     LKRFQKSFPDVAKILSRFTNFITFKDVQYPEALRNTFEPPSVIFFEGDAGLLNSRNIL
                     AIVGSRKADGYGASIAKEYAKSLSEKGITIVSGLAVGIDAQAHIGALEGRGSTIGVLG
                     TGIDVAYPASNRTLIKSVKEKGCVISEFLPGTPPLKQNFPKRNRIISGLARAVFVVQA
                     TMRSGSLITARLALENGRDVFAVPGDINRRNSEGTNWLIKNGAKLISECIDIIDEFPE
                     ISFEEPQSGNLQSRVLDLLGNGPLTFGELLLMTDFSSKDLIVELTNLQLGGYIFEENG
                     RWNRT"
     gene            32746..33690
                     /gene="fba"
                     /locus_tag="PKL93_02930"
     CDS             32746..33690
                     /gene="fba"
                     /locus_tag="PKL93_02930"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNQ70248.1"
                     /translation="MPYVNTKEILERANKEYYAVPALNINNLEFLQAIIDAGVEERSP
                     VIIETSEGAIKYAGNGDIKLGAKLFVSMVESYARNVDIPVSLHVDHGKNFKIIMAAIQ
                     AGYSSVMIDASEFPFEKNVAETKKIVEIAHSLGVSVEAELGRLVGIEDNVAVESHEAA
                     LVDPDEAKLFAEETGVDFLAPAIGTSHGAFKFKGEARLDFARLKKVKDLTGLPLVLHG
                     ASSVPEDIKKLAESYGADFKGAKGVPGEILAESVKFGINKVNTDTDLRMAFIASLREF
                     LSKNPGEFDPRRYFKTPKELVKNVIRERLRLLGCSNKA"
     gene            33704..34933
                     /locus_tag="PKL93_02935"
     CDS             33704..34933
                     /locus_tag="PKL93_02935"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNQ70249.1"
                     /translation="MKILVINCGSSSIKYQLLDAASENVLAKGLLERIGISGSKLSHR
                     KGEKKYEFEKDIADHTEGLELIIEVLQSEETGVVKNINEIEAVGHRVVHGGERFASSV
                     LIDDEVVSEIEANNFLAPLHNPANIEGIVAAKKILPSVPHIGVFDTAFHQSMPESSYL
                     YAIPYDLYRKYKIRKYGFHGTSHRYVAERAAVMMERPLKELKIITAHIGNGASVAAVK
                     NGRSVDTSMGFTPLEGLVMGTRSGDIDPAIVPFLQEQEGLTAGEVNDLLNKESGMYGL
                     TEGRFSDMRDIEERAMKNEPTCKRAHDIYEYRLAKYIGAYAAAMNGVDAIVFTAGVGE
                     NSPYLRASVIDKYLGYLGISIDVEKNKIRGKETMISTTDSKVKVFVIPTNEELVISRD
                     TSRIVKNGLKELKLWEE"
     gene            34989..36026
                     /locus_tag="PKL93_02940"
     CDS             34989..36026
                     /locus_tag="PKL93_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNQ70250.1"
                     /translation="MRGEKAEYYYVVSSGGLILKGFDSEDKVYLEGDLADPLSVVGGE
                     HSGDLLCVGDSAILVGDRDELKEYLVSRPERLEKLFVDVEKDLSLCSGKGPFDLDEFT
                     ASLVAKKQCWLEEYPPLLFGEKSLYRKGIRLIERKDYPSAQEVLRSYLDQYQNSPLSR
                     PVKLFYAFSCFLNDFLEDALASIMDILESAEDEISRIARFFVCHMGLFESGFKFLYRG
                     PRYSSDLFRILKADYRRIRKAGSDRIVFEEGRKAGSVLFLLKGEIALLKKRGDKNSVL
                     FTIKSPSSIGEIQVLSRSKWDTTLKIKSNSEYILIDRDKLVQYLIHKSPQDGFRMVEY
                     LLGYIRQTSAT"
     gene            complement(36033..36875)
                     /locus_tag="PKL93_02945"
     CDS             complement(36033..36875)
                     /locus_tag="PKL93_02945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNQ70251.1"
                     /translation="MNNNSFQRIQELSRVDVVNILNLVFSDYVLRMHWTLESFERDVR
                     ENNISLSESFVMNVEGIHTGIVLLSFREKRARIDLMGVIPGFRREGVGFKMVDEAIRI
                     AKWKGCDRIVLEVPEEDKRAIAFYEKYGFRHRRELITFFIRRPGIGDFSTKESDSGKV
                     VECALNVMDKYKRNPEWEREPASFHHLERYNFDTVIDEQGREIAYCVWQERDSVFYVK
                     DCGPTRAASFKEAIGAICSIAKSRNLTPLFPTVPDNDPLFTEAQEFEPEILLKQSEMI
                     FKIH"
     gene            complement(36868..37257)
                     /locus_tag="PKL93_02950"
     CDS             complement(36868..37257)
                     /locus_tag="PKL93_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HNQ70252.1"
                     /translation="MSEAGHKRSILLYCDSSAFDRLLDLFADVSLIDCKGQENEIVGE
                     ILENGKGVRKEKAGNGEDGLCYAVFDGFSDESLGNAIDTIRHYTGREWVFATTTESNL
                     SWRMNELLIELTEEHNYFRKARQKKVE"
     gene            37446..38432
                     /locus_tag="PKL93_02955"
     CDS             37446..38432
                     /locus_tag="PKL93_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNQ70253.1"
                     /translation="MHIGRFRDVSMRVFWGVVERDLVTPLDIKAESTGDFFQTLEGIP
                     NVIQNFGLTERINLSDLEVLSPITSPCRIICQGKNYLDHLLDTGIRKEDKKYNMFFNK
                     SSASVTGPNSPIVRPKGVRLLDYEVELGLVLKASIDRPINVEFSHMHEYIGGIVIGND
                     ISARDIQLPQSQFFKGKSYRSFCPLGPFIFLTGRNEMKYLESLTVELKVNGVTKQRAS
                     TSDMIYKPWETLSELSEFSDLEAGDVILTGTPKGTALSQKKGLRTWLRRLLGESKRWE
                     IFIREQEKSGRYLANGDVIESRIFSSDGRIDLGVQRNRVVYEEENDHDRNYL"
     gene            38413..39273
                     /locus_tag="PKL93_02960"
     CDS             38413..39273
                     /locus_tag="PKL93_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNQ70254.1"
                     /translation="MIGIICDSVVDLPVELAERDDVYVVPIMVVLGEKSYREGVEISK
                     SEIADYLDSNFARTSLPSPADVTEAFEAMYSKGYDELLVVNLSSGLSGTYNLFKTIGE
                     QFVSEHHSVKVESIDSLSLSGGIAMLVHKAIRMKERGDSLRVIASDLRSRAGIKNIVY
                     FVLPTLKYLKQSGRIGKLQGSVGEILDVKPIGTINSGGIFTLSGRARGMKKAVEKMVD
                     RLIVAVKGKKVLCLALYHSGNDSDTLALVEWAKRKIISLSETLLTGELSSAILVHGGR
                     GIIGVGALIA"
     gene            39547..40536
                     /locus_tag="PKL93_02965"
     CDS             39547..40536
                     /locus_tag="PKL93_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ70255.1"
                     /translation="MKKPTIKDVASLSGVGTGTVSRVLNGGSVKETTRIKVMKAIDKL
                     GYKPNQMARTLAGGKTKRILFLMPEIRTEFHWRVMKSFDEYLDKMNYEMVIYPVFSDK
                     RLKKLRSDPSLIQNIDGAVICTMNIEFLSRKYIDFGTNVVLFESQSDEFDCVYLDNVL
                     GGRMAAELLTECGSSDLFVISFDEENTILATENFEKRILGFTSYLKEKGYEFNEEKFV
                     YTSFFFETAHERIASILSQYDRPGIFALADNFALEVLQMASAMKKIPGKDFFLVGYDN
                     QSWTERAGLTTIEQPMEDMGRKTAELIIQKIENPGQYVQPVKFLPSLKRRKTA"
     gene            40563..41639
                     /locus_tag="PKL93_02970"
     CDS             40563..41639
                     /locus_tag="PKL93_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70256.1"
                     /translation="MARKDKLSLAQKEQRTAYKLLIPALLILALVAFYPLFQVFYTSF
                     TDKVFASGTDEAPKLIGFENYKSLLGLTVMELPKVVDEATGEASIDEETGKPVYERAI
                     RVLPRSPVRYREWFQFSLFGRRYVVGARDPEFMKAISNTLVFAVISVFLETVLGLGVA
                     LVVNSNFKGKGVMRATMLVPWAVITVVSARIWEWMLQPTRAGLFNAVLSKLGWGDGDL
                     SFLSMPALQMPTLIAVDVWKTTPFMALLILAGLQLIPRELYEAARVDGAGRLKQFLSV
                     TLPLLKPTLAVALIFRTLDALRVFDVFQVLMGNRMYSMASYNYYQLIGNRNMGLASAI
                     GVIIFFLIGIFAVIYMRLMGVDKE"
     gene            41636..42502
                     /locus_tag="PKL93_02975"
     CDS             41636..42502
                     /locus_tag="PKL93_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70257.1"
                     /translation="MRKNKLIWLTGKIAFWILVIFIFFYMLFPFYWAVNSSLKSEAQL
                     QMTPATFIPVDSDGRFAPTLQNYVAVFNDGTFVRALWNSTIVAGLTTLLALGVGSFAA
                     YAMGKLRFKGKKLTLYLILSLTMFPQITVLSGLYAVINTLNLGARISLILSYMIFTLP
                     FTTWVLTAFFKELPTEIMQSAWVDGATPFQTFYKILLPLTAPALVTAGLLAFIAAWNE
                     YIFALTFTTIAPEARTIPVAISLFTGSVSRQVPFGEIMAGAVIVTIPVVVLVFIFQRR
                     IVQGLTAGAVKG"
     gene            42624..43892
                     /locus_tag="PKL93_02980"
     CDS             42624..43892
                     /locus_tag="PKL93_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70258.1"
                     /translation="MKKVLLTLMVAMFALVAVSETVITVAAGAVGQELELTKKAAEMY
                     MEIHPDVTVKVLDTPDMVQDRLGLYLQFLEAKSPEIDVYQVDVIWPGDLAQHFVDLYE
                     YNADKVVSMHFPAIVENNTVDGKLVAIPWFTDAGLLYYRTDLLEKYGLNPPATWDELE
                     EAARIIQEGERKTNPDFWGFVWQGNAYEGLTCDALEWLASNDAGTIISSDKKITIANS
                     NAVEILEKVAGWVGTISPSGVLTFAEEDARAVWQAGNAAFMRNWPYAYSLSKGEDSSV
                     AGKFDVSPLPAGKSGNGAATLGGWQLSVSKYSKNPEVAADFALFMAGYEVQKMRAVEG
                     SFNPTIQALYADYEVLKANPFFGSLYDVFVNAVARPSTATAPRYNEVSTLFFKAVHSI
                     LSGTADARNALEELALDLEDLTGFEVVWGF"
     gene            43968..45203
                     /locus_tag="PKL93_02985"
     CDS             43968..45203
                     /locus_tag="PKL93_02985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ70259.1"
                     /translation="MRSAQVNKKLLKDYQPFVEEEVIESIIELSRELKGLRVLHVNAT
                     SFGGGVAEILHTLVPLMKDCGLEVDWKVIDAPPQFFDFTKRMHNALQGKKDFLSEEDK
                     RLFEKVEKDNAAYIGVDEYDVAVLHDPQTVGLPAFANFGNCKLVWRCHIDTSSPNEAF
                     WNYLNTFLSYYDAGIFTLKSYAKDGIAIDKIYDIPPSIDPLSNKNRMLSEEEMKKAMT
                     KLGLVSGRPVIAQVSRFDPWKDPMGVVDAYRQLKRKFPDLQLLLIGSMASDDPEGWKI
                     YEDLLRYIGMDYDVKVLSNFHGIGDIEVNAAQRISKVVLQKSLREGFALTMSEAMWKK
                     TPVIGGNVGGIPLQVHHGVNGYLVESVDETVEYTERILENPDLAKELGRNGYEIVKKH
                     FLCTRHLLQYLELFRDLTR"
     gene            complement(45247..46470)
                     /locus_tag="PKL93_02990"
     CDS             complement(45247..46470)
                     /locus_tag="PKL93_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNQ70260.1"
                     /translation="MKNVVYAQSGGVTSVINASAYGTLVAALESKEIDRVFAGINGVK
                     GILNEDLYDLTEEARSEIDRIPYTPGAVFGSCRTKIDSDEDFEKLFKVFDAHSIRYFF
                     YNGGNDSMETAHKISKKAKEIGYELQVVGIPKTVDNDLLETDHCPGYGSAARFTAISI
                     MEATRDLKSMYSDSTKVFIMESMGRNSGWLAASGSLASTDRFHGPQIILLPEVPFMED
                     SFIAKVEEVVKKEGYCSIVASEALKRPDGSFISTAGYYDAFGNVQLGKIGQYLEKIIR
                     DALKCKVHVALPDYLQRSSRHVASLIDWQEAVEVGALAVREAIRGESGVMISINRLRS
                     KPYLKEYTTVALELIANGTKDLPVEFISEDGFYVTDEFRSYALPLIQGEAPNLYLNGL
                     PMYENLQKIRARKLL"
     gene            complement(46562..48490)
                     /locus_tag="PKL93_02995"
     CDS             complement(46562..48490)
                     /locus_tag="PKL93_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNQ70261.1"
                     /translation="MSVIIIAAIFIVGFILQIRLMQSPKTALLGNRIGAAFMGAAIIY
                     TIVSYGGIGNSALWLWLVIGAFAGVSLGHVVKMIQMPQMVGMLNGFGGAASALVAIAG
                     SSLYHAGTSWYLWLTASLALSVGMLTFTGSMIAAMKLQAIIPGRPVNIDKGGKLQQIM
                     LFVIAVIVFLSTLITGIYVVWVVLLTIISAVYGVIMALRVGGADMPVVISLLNSFSGV
                     AASITGFAVEDPLLVGVGAVVGVSGLILTRIMCKAMNRNLSAVLTGMTTVSNLRKPNN
                     TPHSSSILKEINEKGTRNMKVGETVNSAKKVIIVPGYGMAVAQAQGKVKDLIDCLERA
                     GKEVKIGIHPVAGRMPGHMNVLLAEVGIDYDKLYDMDMINPEFKNTDLTIVIGACDVV
                     NPSANTAEGTPIYGMPILEVEKSKSVIVCNLDEKPGYSGVENTLYSMENVITIWGNAS
                     ETVPVIIEQIDNSPSPSSKRDEQVAKDLPTIISEAKKVIIVPGYGMAVAQAQGKVKDL
                     IDCLERAGKEVKIGIHPVAGRMPGHMNVLLAEVGIDYDKLYDMDMINPEFKNTDLTIV
                     IGACDVVNPSANTAEGTPIYGMPILEVEKSKSVIVCNLDEKPGYSGVENTLYSMENVI
                     TIWGNASETVPTITEACR"
     gene            complement(48487..48777)
                     /locus_tag="PKL93_03000"
     CDS             complement(48487..48777)
                     /locus_tag="PKL93_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNQ70262.1"
                     /translation="MLLALIVFLVACAIIGYRIISSVPSLLHTPLMSGMNALSGITVL
                     GAIAVFATATSGIVLFLSGAALVLAMINVVGGFWVTDRMLKMFDSSARRTDK"
     gene            complement(48806..49987)
                     /locus_tag="PKL93_03005"
     CDS             complement(48806..49987)
                     /locus_tag="PKL93_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNQ70263.1"
                     /translation="MKFSGLTIGVPKEILAGELRVAVTPDVAKKFVENGAKVIIESRA
                     GEGAFFFDGDYSAVGAEVVSTPYEVYSRANLILKVKEPQYNSSIGKHEAEMINRGATI
                     VAFLHPAHDVNHKTIRTLAERDITSFTLDSIPRISKAQSMDALTSMSTVAGYKSVLFG
                     ANRIKKFIPMMPTSAGMIPPSEVVVVGVGVAGLQAIATAKRLGAKVKALDIRPEAASQ
                     AKSLGVEVIPFDVPQQLAMGKGGYAKRLPKEWYEKEREILSKHLATCDILILSALIPG
                     EVAPTLVTKTMVDSMKKGSVIVDIAIDQGGNCELTEEGKEYDYNSVWIIGMLNIPATL
                     SIDSTRMFSSNMWHFVDNLVKDGKICADADDQIVKETLVTLGGSIIHHGTLLAMGEIS
                     E"
     gene            complement(50208..52238)
                     /locus_tag="PKL93_03010"
     CDS             complement(50208..52238)
                     /locus_tag="PKL93_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ70264.1"
                     /translation="MRKWFFWALLMVVVVVVFAIRLLPDMLNKPPIISIPDMIIDEGS
                     TLSIDLHDYSSDEKMTSLSYTKISGPGEVKENFYIFSPSFQQSGSHSVTISVVDPKEQ
                     MAESSFEITVREVNRPPELSIPDQVVAEGETISINLDEYANDPDLDALNYSLTEGNGQ
                     IIGSNYTYEPDFTSANYERVTISVSDGRGEKAVSTFMISNKAFATSATELTADSTSSV
                     PSETAATGLDSPVTSSETEVADAQNLPPTADVPDQTLEQGNTLVLDLRGFVNDPEDEP
                     LSINLKSGPGEVQRTLYSYVSPPGDAGSKTVVLEISDGKNVVEESFQVNIESANRAPL
                     ASSIPEGVVFQSEQYRLNLENYFNDPDGDSLSFQLLNGPGRIEGSTYTFQSNEPGRYS
                     VTIRASDGKGEFIERTANLTVVRTNNPPTISILPRRIAEGDVLTIDLSEYASDPDNDL
                     LSFSLVSGPGTIQGETYTFDADYGTAGTYEAVVSVGDGKGGSARATLRIDVRESNRPP
                     ELNIPGFTIAEGSRLVVDLSEYSSDPNNDSLSFEIVQGMGTIEGSRLIIEPGFEDYGF
                     YTIVVTASDGRGGQTSSTFDLLITDTNRPPEFNVPDQTTIATRTLRLDLRQFSIDPDG
                     DYLRYELVYGPGVLTGSIYSFIPEELGSNTVMLTANDLKGGEATATFTITVR"
     gene            52536..54296
                     /locus_tag="PKL93_03015"
     CDS             52536..54296
                     /locus_tag="PKL93_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNQ70265.1"
                     /translation="MPVTITVNGKDFTVDENQSILEACREVGIAIPTLCYHPALSVVG
                     SCRVCVVEVEGARNLSPACATQVADGMKIKTNSERVNRAIRFNLGLLLSNHPNDCMTC
                     DVNGRCEFQDLIYQYDVEDMFPKTLRNVPYDFSSPSLFRDMNKCIDCGRCVRACDELQ
                     GLSILSPVNRGYHVLPLPALGAPIAATDCINCGQCSAVCPVGAITERMEFREVVAELR
                     KHDKVLIAQTAPSVRVALSEEFGELPGTVSTGKMVAALRKLGFDYVFDTNFSADLTIM
                     EEGSELLERVKEGGKFPMFTSCCPGWVNMMEKLYPEFTENLSTAKSPQEMMSALVKTF
                     FAEKIGKRPEDIYLVSIMPCTAKKDEKGRDTLTVRGARSTDAVLVTRELGKLIKMHKI
                     PFESLEEEEYDSPLGISTGAAVIFGATGGVMEAALRTAYELHTGEELPRLDFDFARGF
                     DGVKEAEIDMKGTKVRVAVVHGGSNVVKLMDKIRSGEAFYHFVEVMACPGGCIGGGGQ
                     PKSSQPGYLQKRMEAIYNIDKASVIRKSHENPAITKLYEEYLGKPLGHLSHELLHTHY
                     KDNRKSVIERKKELEEIAKG"
     gene            complement(54351..55265)
                     /locus_tag="PKL93_03020"
     CDS             complement(54351..55265)
                     /locus_tag="PKL93_03020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNQ70266.1"
                     /translation="MMNSLFYLIDLLLALPLLMNGLGAFIAACCASVLISFSMRGYEG
                     ITISDKEKQMASTLGSAVMSCFVFPVFLPVSPFVALPALGATVVRNFIIYKRVKSQFK
                     EESFMLTSNDPIEPKVAYDSFKRLLDVGIGVILLVLFSPVTVIVSLISLILEGRPIFI
                     CQTRIGKDCKQFGMYKFRTFDTKGNKETITKLGKFLRPLRLDELPQIINIIKGDMSLV
                     GPRPELPSFHKLGMDNIEDYSSRLLVKPGLTGWAQINYKYTTTLEEYRIKTAFDLYYV
                     KNRSFILDIKCLFKTPFAVFLTLLKKED"
     gene            55367..56656
                     /locus_tag="PKL93_03025"
     CDS             55367..56656
                     /locus_tag="PKL93_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70267.1"
                     /translation="MKKLYLSDLHIGDGTLKDDFKFDSDLVELILSSKEKAFREIIVV
                     GDGLELLTSEFVKKRGLLPFDQLIDQLDVSIIDVIENRHREVFRAFREFTKSGRLIYV
                     VGNHDSFLLFNEGLRKRLTEFLGDSESVQIKPYYLDREFKTLALHGNQYDIVNRFFKD
                     RRSGELMQPFGDYMARFMMKNFDKCLLRRELPEQAVRDYQNIHPTLDVFQWFDFIRRV
                     YDVDSDLQDEWARQFLLMLKSDAAQRWIDANWPMLKVIEKLFINKHGGMKLGSLMIRI
                     VMAFRKLKKTDNLYKQGRRLLGAEGKKGFRKAMNNEFFLMYGDKAPAIVPGELKGVIM
                     GHNHRHVLRMISSNGEEKFYANTGTWKPVVEMIDDDPRNGFARRVELGYITIEIVSNE
                     FLVESKHIKKLSGLRFSEDTLDKKTEWNSVREIGMRV"
     gene            56670..61127
                     /locus_tag="PKL93_03030"
     CDS             56670..61127
                     /locus_tag="PKL93_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70268.1"
                     /translation="MHYYRGSSIVPIIITLLLVIVAAVVAAFFLWPVPEPKILISNVS
                     PIPKNAERIQYAEKVELRWEAVYERPRHGLLAEVFAGKDRNSLTRLALDLQGDLTSPT
                     EGIFSFSHVFEADPHSQYWWKVRVYTEGGKSAESEIWTFVLMNTYPRRPELVPPSSNL
                     GNVPLKDLTLNWSSNDPDGDELVYDVYFGREPRLDEKDILIRGTNETMVDVSLLKRLD
                     YSTKYYWKVVAKDSYGGESTSYTESFTTQMRADLPAVTPENPRSGASDFDAGRGFLSW
                     KTSLPLEYYLDELDFDVYLSEGQSRYSLIGTTNSTEIAVPSLKGHTTYSWYIVVRDAS
                     GKEKKSEEWIFRTSNRAPIIEMEYPNISPGQDSVPVKWEVIDPDGDSVTCEVFFGPDG
                     DEPARIASGRMTSIFLSALDEQTNYTLSIRASDGQGGETIRDISITPGNRPPSIYLSR
                     PVSMEFVEPSVVSFNWFGEDPDGDSLNYMLHLKSKNDSRILGPIESQEYIVRDLEENE
                     NYSWFITVEDSRGAFARSEDAVFTTGQKSLEVAVPLTPQPGEVEVSLSDTTFSWEIDD
                     ESEDLSYRFVLGNDDKLNDIIFENETKETSIELTLDLLSNKKYFWRVDVIKNGSETEG
                     EVWSFTSENLPPALPELRFPVDGASGVLTNDLTLSWVSSDPDGEIVSTKVHLRDSAGN
                     EEEFEALGNSLTIEELDPGQRYEWAVKVTDDGGKSSVSEYASFVTGNQRPVITLTHPP
                     RMFLEGATTPLVVEWELDDPEGEDLEVGVYVNLAEESLGRPVAMGNNLERYVITNLIS
                     GRDYLLTIMAEDPGGEIGTLEMPVKSRVSGLDYIYPSGGVFENGREFSWSYSQQNSGY
                     VFRLYDSDHNPIVRRVVQDNKYRPELPFEDGRQYFWAVSVLRDNVEYAGEPVSFISGQ
                     SFGVLLEAPRNGEKVPASGVTLKWVLDGDTEAIRQKEVYFGELGSLSRYTVSGDSYHT
                     GRLLAGRTYEWFVQLTDNSGATIRSETFRFSVENRPPELSLISPVNGRAVGETYVDLT
                     WSGYDPDGDQLRYSVYFGTDEDLELYRDSMQSNQIRISGLLMNKTYSWFITASDGQNV
                     VKSETRTFNVTAPKFKIDILSPVVDSEVDLTPIFSWQSSEVRSGEFRVFIGESESEMS
                     LIGITDATSMRLQNRLDNGKTYYWSVELWQEGKMISSTGPNRFKTMSSERPIASESVD
                     TLAFYFNEVLNIVSLSSAGLEIVEIPYQYDGKNSAILNGDLLYVIDQSGKLSTINYRT
                     GEAKVIDTLVTNTVPEKLIMEGGYLWILDTKSAGMVIRVVLNESGIPERMESVYRDWT
                     TPVDLFVTQDLSRIYLADALSGIKILEREGETYNDRSSSFEAPLDGYSRAVAESNGVV
                     YSGEAGIDGGLKVIDITRSRKTNVGRYYIVLEIEVSGSVLYASTDKGVSVVDISTPDS
                     PVILRDLELTQVDEISVEGRLLIVRSGNSMLIYDVEKPNDPVLLESRKE"
     gene            61151..62647
                     /locus_tag="PKL93_03035"
     CDS             61151..62647
                     /locus_tag="PKL93_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70269.1"
                     /translation="MKKLLLAVLVTFLSVAVFGVSDSVLVMAIETEPVGFDPTLVTAF
                     ASHRVLENVYDGLLKYDENMNLVPNLAEDFEVVDPYTIVFKIREGVKFHNGETLTVED
                     VLFTFERIRDPEVKAPAASYYGEVETIRSFDGNKVEFKLKIPMASSLLPNFAGVNSAI
                     LSKSFVESGANLQLETNGTGPFYIAEFVAGNYIRLRKNADYFVAGLPYLDEIKMMIMP
                     EEVTRVSALRNGDVDIAKISEPLSLNQLASDKFVIYRTPVLSYYLLGFNTTRGPLSKP
                     EVRNALSYAVSREMIVKAVAFDEATVTGPLNPELDFWALQPNQFEEYSYNPSKAKQLL
                     AEAGYPNGFEFEIVAAQRYNFDKVAQVIQAQLAEIGVTAKINLVEWGIFISKWRESDF
                     DSFISMNSGSIDPDIQFNRTFRTGGSTNVFLYSNPEVDDLLDRGRSESDMNARKLIYE
                     EVQRKLVEESPILFLYSPNTLFASNDSVKGFRSLANESLVFLRETSKD"
     gene            62662..63615
                     /locus_tag="PKL93_03040"
     CDS             62662..63615
                     /locus_tag="PKL93_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70270.1"
                     /translation="MEFGYVIRRLLSAVPTVLLVTFMVFIAIHIVPGDVIDIMLGTQN
                     YLTESQIEDLYSEYGLDKPLIVQYGIWVKNLLSFNLGTSLRTGRPVTELIKERFPVTL
                     ELAAFSLGFALIFGIPMGIISAVKRNGFLDNFVRVIGLIGLSSPSFWVGAILIVLVSG
                     AFDNFNLFGYVSPVVDPFSNLQVMFLPSLTMGLMVAAQILRITRTSMLDVLNQDYVRT
                     ARAKGVSARNVILKHSLRNALIPVVTLSGIQLGYLLGGTIVIENMFALPGMGRLLLQV
                     VNERDYPVVQGIVLFIGIIIVMLNILVDVIYTLIDPRVELR"
     gene            63627..64457
                     /locus_tag="PKL93_03045"
     CDS             63627..64457
                     /locus_tag="PKL93_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70271.1"
                     /translation="MLRVMRRLVSNPIYTISMIMIFVIVLITLFPGLFAPYDPYKMNM
                     DAFMSRPSIDHLFGGDQFGRDVFSRSMYGIQKSVIIASSAIAISAIIGTMLGLLSGYI
                     GGLTDLIIMRIMDSFFAFPSLILALFIIALFGSSMSNLIFAIGLVYVPIFARTVRGAT
                     LSLKDTLFVKASKALGKKNISIMLKDILPNISSILIVTFTTNVSTALLTEASLGFLGL
                     GVPPPEPTLGGMVGQGTSYLLSAPWIVLFPGMMIAIIVLSLNILGDGLRDVLDPRINR
                     "
     gene            complement(64458..65000)
                     /locus_tag="PKL93_03050"
     CDS             complement(64458..65000)
                     /locus_tag="PKL93_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HNQ70272.1"
                     /translation="MLKGIIAALWNRKERPESVSPETVFFLNGGLFEIQERIDRFKAA
                     GKKVFVDIDFVSGLSGDEDSVLYLKKSGVDGIITAKLRIFKQAVNAGMHQSLLRFFVL
                     DSRAVEKGIQTIVSNGVRNIEILPGIVAAKVAPKIRVHAPETTIVAAGLIDSVEEIEM
                     LKNHVDGVSTSSTALWTYRW"
     gene            65189..66631
                     /locus_tag="PKL93_03055"
     CDS             65189..66631
                     /locus_tag="PKL93_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ70273.1"
                     /translation="MQAVVIGGGVVGSLIARELSRFRIKVVLVEKSEDIGQGVTKANS
                     AILHGGYDDPPGTLRARFCAPGNLMFDKLSEELKFPVKRTGSIVVAKDRNELPKLEEL
                     LQNGINNGVKDLRIVGKNELRELEPHISDEFNYALFCGTAGITEPWMVAIASAMNVAG
                     NGGEVITGEEVIGGKINEGRVTEVFLSSGRKIEADLVINAAGLFYEKVASAFNVNVPP
                     VHLRKGEYILLDKKASELVNVIIFPLPTAAGKGKLVVPTVDGGVLLGPTSVELPEFSP
                     EDVSTTDSGLTSVRKSGEYLIPGIDNPKWFIKSFAGLRPETVQKDFYIKRAEELANFI
                     TVGAMRSPGLTAAPAIAEFVVSTIVPETGIDLVRRDDFVPCLEERVKIKELPAGKVSE
                     MIDENPAYGRIVCQCNEVSEAEIVQAIRDGARTIDGIKFRTRAGFGRCQGGFCSWNIA
                     KILARELKKDLSEVRQNSEESWIVDGKVRQ"
     gene            66628..67863
                     /locus_tag="PKL93_03060"
     CDS             66628..67863
                     /locus_tag="PKL93_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ70274.1"
                     /translation="MNRLTTDVLVIGGGAAGMSSALSAAKTGADVTLLEREPFAGGVL
                     NQCIHNGFGLQFYHEELTGPEFASRLQREMSEEGVSMIGESYVRHIDVREKKAVVLSP
                     KGAFEISAKALVISSGARERPFGSLLIQGYRPSGILPAGVAQRYVNLENYLPGKRAVV
                     LGSGDIGLIMARRLTLEGVEVIAVIERMPYPGGLTRNIVQCLEDYDIPLLLSTTVTKV
                     FGKGRLEEVEITKVDERFVPIPGTSSRLKVDTLILSAGLLPQVEDFDDDLTIDPINRG
                     FVVSNTGESSVEGVFAAGNNVAVFDLVDYVAAEGWIAGRHAALYALGKNTSGDRVPVV
                     RGDNVGVVVPGIVDMDEHVRLYVRLKNPMDEGTLVLKELGLEKSFSYGVPSEMIQMVI
                     NKEKLMPLIDAKRLTVEVH"
     gene            67863..68237
                     /locus_tag="PKL93_03065"
     CDS             67863..68237
                     /locus_tag="PKL93_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HNQ70275.1"
                     /translation="MERHITCVICPVSCKISVRKSGSEYEIRGNRCPRGKEYALNELI
                     MPKRILTTSVKVINGEIPLVSVKTSGAIDRSIIKEKMDLIRKIAVEAPVQIGDVLMDD
                     IDGQGTALIATRPVKRIQTFLP"
     gene            68287..68361
                     /locus_tag="PKL93_03070"
     tRNA            68287..68361
                     /locus_tag="PKL93_03070"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68319..68321,aa:Asn,seq:gtt)
     gene            complement(68387..69325)
                     /locus_tag="PKL93_03075"
     CDS             complement(68387..69325)
                     /locus_tag="PKL93_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase A"
                     /protein_id="HNQ70276.1"
                     /translation="MESIGRLSSRVLEHLSDPVVKSAVKVKSTILGEASSFLRGKNFV
                     ELLPTIVSPITDPLNHEVHTTRFTYYDQEYRLTQSMIFHKQLACKSFERIFIVSPNVR
                     LETSEKALSGRHLFEFSQIDLEIRNAKREQIMSLIEDLIITVMLSVKRNCSEEMDFLS
                     SDPSIPSKPFATVKFMDAYEQYGKDFETILSASFSEPFWLIDFPIWEREFYDLLSEDK
                     RTLKDMDLILPHGFGETLSGGEREWEHQRILERMGFKGNDPEELSWYMDIAEGGELVP
                     TAGCGIGVERLTRYVCSLDNVSRTRLFPKVPGQSWI"
     gene            69589..70602
                     /locus_tag="PKL93_03080"
     CDS             69589..70602
                     /locus_tag="PKL93_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ70277.1"
                     /translation="MNIIPIFVPHEGCRTRCIFCNEYSATGVTGVPDEKEIISTVEKY
                     LSFFRHPEDVELAFYGGTFTGFGNQQKYLEVATEFFKAGHIKGIRFSTSPGLISERLI
                     EYLKPYPVNFIELGVQSFDDTVLEKSNRDHNTSDVYKATHLLNSNGIKFGIHLMTGLP
                     EDSESKDIHSTREAIRVGASSVRLHPLVILKGSLLENDYKLRRFSPLDLQESIEILWK
                     MYLLLSTSNVKINRIGICLYGKQIDNVVAGPFHPAIGELVRDRLFLEIVRAFSGEMKR
                     NELRLHNKFKPFFTGHKKGIVEEARKEGILIDFAHDGEEIDVSLYMRRIKERVLEGVY
                     AEA"
     gene            70589..74113
                     /gene="smc"
                     /locus_tag="PKL93_03085"
     CDS             70589..74113
                     /gene="smc"
                     /locus_tag="PKL93_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNQ70278.1"
                     /translation="MLRLNSVYIKGFKSFAMPTRLEISSGMTAVVGPNGSGKSNVVDA
                     IRWIFGEQSMKNIRADSREDVIFNGSERFPPSNSAEVKLVFESEEGIFSIAREISRDG
                     QSSYKVNDKQSRLKDIKELFQGTGVGMDIYSIVGQGQVDKVVTASPYELRALIEEAAG
                     TAVYKERKKEALGKLAATEENLSRLEDIIFELGKQRKSLYLKAKRAEKFVEYSAKQKE
                     LKNLFFGNIARLEEEKLQTLNGDLREVRDELKDLQKKLIEGESKWSALRAEFSEVDKE
                     IEGFTKLLEEYKKRQNDLLELKEMYSRRLNEKENRLIEISTRIDNFRSEIEDLDKRKD
                     EIKLIIESLEQQVSDEETALSLSEEERDSLLKNYSAREREWLKHQETFESISKRLSKI
                     ENELERLENSREDTSKRLRLIENQLETKRDRFETLKEETESLAKQGKESSDKQRKVEA
                     EVSASRERLVELDAELEKARDRLSSNESEFRRSQMERSLLQRQQEEYQGFSKAVREVF
                     SRKESFAGLRDVVANIIQVPESFETAITVLLGYRMQDIVVDDSITAKRIIEFLKSYKI
                     GRVTLLPIDMIEGSFRNFSRVESHPGFVNYAARLVEIPDGFEKLPVYLFGNSIVARTL
                     DDAIEMRRSEGFIGRIVSVDGQLLSSGGSITGGFIGEETRTDLLSRKRRIQELVERED
                     ELEKQIQLGNKQIERLRDEMTEVRGYLKALQEELNELASKGAAINRMIAELLKSAQEI
                     EEEIAELQKLENEYTRRMEENSRKKEVLVGEQSALKEERSELQRKVEEESEELKKQKK
                     ALEQLQEKIVDTRLRLSTLYEKQEQYTKEHSSLIQKSKADQENIDLLSREVSEVESET
                     ERLRRQVADQERELTSVKKETEDLFSSIRNQREGKEQRLSALQEAEEEINEMKAEREK
                     LREKSHKLEMHIQESEMKLSRVREELDEVEKEVEILSEEKLQDIKVELDDYENKLKFL
                     GSVDLEAIDEYGVVDKEYQELDEQKKDLEEAKVKLIELIDKTDAKAKNIFMETFNSVN
                     KNFSRYIEEIFDGGEGEIKIIPGEDLLETGLEITVRRPGRKIQKLQLLSGGEKALVGI
                     ALVFSLLSIKPSPFYVLDEVDAPLDDFNAERFRVLLKKHATDTQFLVVTHNKLVMEVA
                     NVLHGVTMTDGLSRVIPVELQSVETVIG"
     gene            complement(74103..75008)
                     /locus_tag="PKL93_03090"
     CDS             complement(74103..75008)
                     /locus_tag="PKL93_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ70279.1"
                     /translation="MKKTDRRSLGLVILATTAVIWGGSYIFTKVAVSSLPPMLLAFLR
                     FAIAVAILFPISSKKRGSFNTVDHKNAALAGLSGITFYFFFENYGLTMTNAADASLIV
                     STAPILTILLYDLIRKKYDLFEYLGGLVAFSGLSLIIYSGRFADGSSVTGNLFSFGAA
                     VSWTAYTYFYEKIHNSSIWTTLEIMLWGLLFSAPFALSEILLFKKPVVFSLGAVSGIL
                     FLGIFASALGYIMWNKGIDLWGGKAATLWVYTIPVFTVIADIVFLGNLPSMLFFAGSA
                     LVGAGMLLVIGREYRRLVSHDNTNR"
     gene            complement(75005..76492)
                     /gene="aglB"
                     /locus_tag="PKL93_03095"
     CDS             complement(75005..76492)
                     /gene="aglB"
                     /locus_tag="PKL93_03095"
                     /EC_number="3.2.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047798 - cyclomaltodextrinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase"
                     /protein_id="HNQ70280.1"
                     /translation="MKNWQRDSIVYQIFPDRFKIGNGKNVFQKREQGHYQLPKQDVRD
                     WGEKPVASVDGSHQYDFWGGDLAGITEELDYIRDLGANTLYLNPIFWGHTNHKYDTVD
                     YFKIDPDFGTEEEFLLLVETAHSKGLSIILDGVFNHVGASGKWANSLELFEEPGALQG
                     NEEFRKYFFEGKKGLRGWMDSSNLLELNLENPILSNMVFRGTDSVVAHWLKKGIDGWR
                     LDVAYDLGPRILKEIVATTKSIRSGAVVIGEIWNYPGGWPEQSHLDGLMNYYFRLVIW
                     EALNGYLSGHDALKIIDEAIREAGLTFISNSWTILSSHDVPRLKNELGSFQRVKAALV
                     LQYCLPGIPLIYYGEEIEMDGGNDPENRAPMDWQSVSKETETRILYKKLNAIRETKAS
                     LRAGNFKPLISSDSSLISFKRTGEIVDDMVLCVVNPTANNVESRVFLQEGFLMNGTEM
                     KDLLTGRAFRVSFGTVSVDLGPFEALIMEPLIFRSDLHYSPYKRL"
     gene            complement(76489..77301)
                     /locus_tag="PKL93_03100"
     CDS             complement(76489..77301)
                     /locus_tag="PKL93_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNQ70281.1"
                     /translation="MDKGKKIDMIVLSILLASITIFSLILTSLSAKNRLDKVAALSVL
                     YNAGLGADYKFILESPSYQYDDRVVEAYRYFTDKSGSLNYRLSSSVKMHNVSENDLFV
                     CNKIISDLSQQNAKRKCPYLETKIASLIESSSLLSDRSAIFKNRLSEEIYNALMEFAN
                     VKVDIIVGGEIKTLDLSRLDPEVVLSIMVVESSLNPFALMEERSIDESFSDYVHSRGL
                     MQIYEMTLWTLNSWLKQSGINIKPQELWSIRNNIFLGMVYLAYANEFLEEKR"
     gene            complement(77368..78990)
                     /gene="nuoF"
                     /locus_tag="PKL93_03105"
     CDS             complement(77368..78990)
                     /gene="nuoF"
                     /locus_tag="PKL93_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNQ70282.1"
                     /translation="MSKPITVLVSIDSNSLLLGARHLKNYLKTLVENYNLSSMVEILE
                     TGSFGEYGRGVLFLVLPDDVMYSAKSEVDIEKIVLEHLLKGRKVRDLVVADFDSGGSF
                     EATKSTSEVRIVLRNAGSIDPRNLEEYIALDGYQGLAKALRMTPEEVIEELKKSNLRG
                     RGGAGFPTGLKWEFTQKVRSDEKFVICNADEGEPGTFKDRLIMEGDPHSVIEGMVIAG
                     YAVGASKGYIYIRGEYFESVSSLRDAINKAYEYGLLGQSVLGTEFSFDLSVRLGAGAY
                     VCGEETALIESIEGKSGRPRLKPPYPPVSGLYDKPTVVNNVETFACVPPIIDKGAGWF
                     RSIGTQSSPGTKVFSLCGDLVKRGIVEVPMGTSVRDLVHKFGGGISKGRTLKMVQTGG
                     AAGTFIKPEEMDAELDFDSFKKSGASLGSGVILAIDETHCAVDIAKNLMEFFAHESCG
                     KCSPCREGTRVIVHILNEFSKGRGTVEMFNQLYDISETMEESSFCGLGQSVPIPLRSI
                     LDRFKDEFLSHLNSPGCPVGVCEFDKPEKKKR"
     gene            complement(78993..79490)
                     /locus_tag="PKL93_03110"
     CDS             complement(78993..79490)
                     /locus_tag="PKL93_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNQ70283.1"
                     /translation="MPAEDVRLTVTRIYEDISGQSLEKGDVLINTLHAIQDHYGNFIP
                     IEAAEVLKELTGTPLSRIYEVLTFYTMFSTHKRGKYVVRVCKSLPCHVTGGQAVIDSL
                     KNTLEIGFGETTEDGLFTLEETSCLGLCGVSPVMMINDEAYGNLTPRRVSEIIREIVS
                     RERSE"
     gene            complement(79559..81358)
                     /gene="nuoF"
                     /locus_tag="PKL93_03115"
     CDS             complement(79559..81358)
                     /gene="nuoF"
                     /locus_tag="PKL93_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNQ70284.1"
                     /translation="MPAVENTVLICAGGACISAGEKSVKGAFEETLRKYSLDNVVRVV
                     ETGCMGACDLGPMLVIYPEGVFYQKITPENASRIVEEHILKGRVVEDLLYRGDAGDLR
                     EKPQEELPFFTEQVRIATRNLGVIDPLSIDEYIARDGYFALHKVLFEIGRDDVIEILK
                     KSELKGRGGAGFPTWMKWDFTKKAEGDTKYVICNADEGDPGAFMDRAVLEGDPHSIVE
                     AMTIAARVVGAKKGFVYVRAEYPLAIERLTHAMKMAREYGFLGENILGTDFSFDLEIR
                     IGAGAFVCGEETALINSIEGKRGIPRVKPPFPANKGLWQKPTLLNNVETYANIPPIIN
                     NGGEWFSQFGVEGSRGTKVFALAGNVKNTGLVEVPMGITLRKLIFEIGGGVPGGKKLK
                     AIQTGGPSGGCIPLELIDTPITYDNMKKLGTIIGSGGMIVMDEDSCMVDVAKYFLEFT
                     VEESCGQCTPCRDGTRRMLEILERITSGEGTMEDLQLLKDLGENIMATSLCGLGQTAP
                     QPVISTMRYFWNEYEAHVKEGRCPAKKCKSLTRVVIDKEKCVGCTACSRVCPVNAISG
                     SVRKPHEIDPEICTRCGSCLAVCRFGAISKVSP"
     gene            complement(81376..81753)
                     /locus_tag="PKL93_03120"
     CDS             complement(81376..81753)
                     /locus_tag="PKL93_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNQ70285.1"
                     /translation="MAKIKSLEELMKIKENAMKGLKMRNSGKRGKVIVAMGTCGIAAG
                     AKDTLRAIVDSLEEKGIDDVSVVQSGCFGLCDVEPTIEVHLEGSDPIIYGHVTPAQAK
                     RVVEQHIVGGKVVGDLIVKRGEL"
     gene            complement(81753..82247)
                     /locus_tag="PKL93_03125"
     CDS             complement(81753..82247)
                     /locus_tag="PKL93_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNQ70286.1"
                     /translation="MSLVNCPQCQQLYKELDEYIDSVKGNTGVLINVLHKAQEIFGYL
                     SEEVQQHVSEKLHIPLSQVYGVVTFYNFFSMKPKGKNQIKVCLGTACYVKGADKILER
                     LQDELNVEMNEPTSDGLFSIHAVRCLGACSMAPVVLIGEDDYYGRVSPDEVSKILDKY
                     RRAE"
     gene            82378..82890
                     /locus_tag="PKL93_03130"
     CDS             82378..82890
                     /locus_tag="PKL93_03130"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNQ70287.1"
                     /translation="MDLESFIRDVPNFPKEGVVFKDVTPLLKDPLAFRECILKLSEKA
                     MKVDFNIMVAPEARGFIFAAPLAFELGKALVPVRKPGKLPYKTKEIEYELEYGRATLQ
                     MHVDAVNRGDRVLIVDDILATGGTMQAIAEMIEESGGEVVEILCLAELGFLEPRKRLS
                     KYSVETLITY"
     gene            82905..84269
                     /locus_tag="PKL93_03135"
     CDS             82905..84269
                     /locus_tag="PKL93_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNQ70288.1"
                     /translation="MLKYDFSFAFKETLESGLAFDEIFDVGKALSANFEKVFQPLPGF
                     LRILDDDEILEEVGAFKSWLQRFENFVVIGIGGSALGNQALHSSLKPINWNSLGNEKR
                     GGKARIFLLDNVDPDLIASVLGELDLAHTVFNVISKSGTTAESMANYLIVRGLLEKLD
                     LPVKEHFIFTTDKDNGVLRQIADNEGIRSLTIPDDVGGRFSVLTPVGLLSAVAEGIDI
                     EQIYEGARFGKERYLRDDLKSNPAAVCALIHYAYLKKGHNISVMMPYSNRLYTLADWY
                     RQLWAESLGKKFDLSGRIVHTGQTPVKSLGAIDQHSQVQLYNEGPKDKIVTLVKVEDF
                     VNQLTIPFIHSDIEALSYLNGIGVGELLNAELRATSIALAANGVPNMTIIFPQIDEVH
                     VGEFIISYEIQTAIMGFLLKVNPYDQPGVELGKKLTYALMGRKGYEEIRDRYEEKTSW
                     RFEL"
     gene            84266..84523
                     /locus_tag="PKL93_03140"
     CDS             84266..84523
                     /locus_tag="PKL93_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70289.1"
                     /translation="MKSELLRVLEGFSVEEVFYTSGEPIPTFVIVSMESEDLLKKIGE
                     MEEIEADIIVISPEEKKELKNASSELSRVVLNVIESGEKLL"
     gene            84523..85095
                     /gene="coaE"
                     /locus_tag="PKL93_03145"
     CDS             84523..85095
                     /gene="coaE"
                     /locus_tag="PKL93_03145"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730308.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNQ70290.1"
                     /translation="MIVGLVGKAGSGKSTAAERLAKSKIKLISLDKLGHDALREERES
                     LVDSFGRGILTCGNVDRKKLSRIVFENSDLLTKLNKIVHPVIKQKALQIVGRDFSDHY
                     IFDGALIHEIGLAEYCDKIIWFECPNEIAIERLMKRGMSKSRAESILASQQYLDSIKE
                     SVDAVVTTLGSIEETFEKLRSILFEWGIVV"
     gene            85134..86399
                     /locus_tag="PKL93_03150"
     CDS             85134..86399
                     /locus_tag="PKL93_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70291.1"
                     /translation="MRKRLILALLIVSFAVFTFAVTEIEFWHAMGGAQGTTVNEIVAS
                     FNEANPDILVKAIYVGNYSALQQKLLVSAESKTLPALSQAYGNWTARLIPRDAVQELN
                     SLISDPEVGLTDEEWDAIWDPFKKMVTWGDTVYALPFNKSTYVLYYNTDLFEQYGLEV
                     PKTMDDLLFAAMMLTEDKNNDGQIDQYGFGFRTTIDHFMIFLRANGGEAIDIKPDGSV
                     EVTINSPEAKEALQLMYDMAHTYNVALFQGGYLDAPFGDGQIAMFVETIASASYVDSG
                     SRGKHGWAWAPVPMWKVQAPPFAGTDVVMFKGISPEQKAAAWKFMKYLISPEIQAYWS
                     VKTGYMPVTEIALTTPQWKAYEEQNPTVMVPISQIPYGSVDPNVALWDDVRNILGTMV
                     GDVINQKRTVEEGLAWAEQEILLKVAEQQ"
     gene            86643..>87700
                     /locus_tag="PKL93_03155"
     CDS             86643..>87700
                     /locus_tag="PKL93_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ70292.1"
                     /translation="MKKYFHVFNFYAFSFSILLYLLNSSINTKAAEWGLSYMVIGLIN
                     FIGCVFYVISALVFGRMGDRTGFKRSLSVGMFIMTIALFLGFFWSRPVDIVIAAIGVN
                     LFFGFFFPSLEGLLSKSEKSIGIDSASTVVRFVLSWSAGNIVGMAFGPFLIQRFPAAI
                     FSYGIALCGFGSIHVRSHLKRHGESLPGSFAETFSSSFREIDLPRLEEFRKVYRFTFL
                     LAGIIYTSGMALFPKLISSTGIQLQNVGFLTVGANVGVFLSFVFLSLFRFWVGSPGLS
                     FITMISVFGAAVATFFLPESPVTFFLATLFSGATYAVPYIFAIFYGLTSKEDDHGKQG
                     GIHESMVGIIFGVGPLLGG"
BASE COUNT        24636 a        18500 c        20168 g        24296 t
ORIGIN      
        1 aggatacact tcccaaccta ttctgttgta gtaagcagtt ggatgaagag ctttgttgag
       61 tttgtaatat acgggagtcc ttgtggagtt tgatctattt ccatttaaat aatcctcaaa
      121 ccacaagtca ggcatattga acccaagact cttctcgaag acctcaacca atacatctgg
      181 tatctcactt acgtgtttca cgatttctga actgtagttc ccgtgttctc cacttacact
      241 ggttccggag attttagcag taattttgat ttcgaagttt tcgttgactg gaagatagac
      301 aaccgctttc gagttcttgg tgctcggtat tactttaacc agatcgtttc tcgatgtcca
      361 gctaccatat tgaggtttga cctctactaa ccactggtag ttagacgatg gatctggagt
      421 aaacaatagc gctgctgctt caataacgac aggttttccc ggttcccatc cgattttctg
      481 ctctagaagc aaaatacttc ccttaactgg gaaacaagaa gaaattgcaa acgcaactat
      541 tataagcgca cccagcatca caaacaactt tgctcttttc atttccatcc cctccctttc
      601 aggtattccc tgacaaactc atcttcctta tcttttgtaa gaccgtccag ccttgcacag
      661 aacagctcgt ctagaacatc tcttatctcg gggccttccc tcatgccgta tctggcctta
      721 agtatattcc cgttgattga taatttcgtg tcattcagtc tcttcagata ggcaaggaat
      781 aacaaatcct cctgttcatc cagataactg ttgacgaagt taagtatttc gggcttgttc
      841 gtcttcagag atctgtagaa ttgagaagga gtctctggct tctcattaac tattatacct
      901 gcagtggcta tcgcatcatt aagcctttca agatagtttt tcggcaatcc ataacgtgct
      961 atgcaccatt caacggattt cgagtcactc tttcttagga ttatcattaa gagtgtgtag
     1021 aacatcctat cttctccaat tagcaaagcg tttctctctc ttcgaaggaa atagcgctgg
     1081 agtctgatgt ctgtctctct atcgaattct gtttcgggga agagcttctc cataacacca
     1141 aggctggcaa gtcttctcaa ggccttgagt ggaagcggct cttcaattgc cttgctcaat
     1201 tcgtctctca gtctctgccc tgttacccta tcgagatatc cttcagctgc acattgcttg
     1261 agcagctcgg cggttctcac ttctatctcg aacccaaagc gctgttcaaa tcttacggct
     1321 ctgaggattc ttgtcgggtc ctcgatgaag cttagagggt ggagtgctct tataatccct
     1381 tttgaaaggt cctttctcga tccaaagaaa tcgatcagaa ttccaaaatc ctcctggttc
     1441 agttttatag ccatggcatt tatggagaaa tctctcctgt aaagatcttt ctttatcgtg
     1501 ctcatttcga cctcaggaag aatcgctggt ttcttgtagt attcagttct cgccgttgca
     1561 acgtcaattc tcagaccatc gttgaagaac atggaactgg cgagaaattc cctgtgctcg
     1621 acaatcttaa tgttaaagta ctttttgaag gtctctgcaa agagattggc attgccttcg
     1681 accactatgt ctagatcgag attttttttg ttcaagagaa gatccctcac aaaacctcca
     1741 actatgtaag tcggcatgtt gagctcgctc ccaactgcac cgagaaatct tagcatggtt
     1801 actatgcgtt tttcgacgcg ttcctctatc aaatcttcta cggggaattt ggcatagttc
     1861 tgatcaccta tcttttctcc gggatcgatt atccttcttc ttccaaaagt gctcttcagg
     1921 agatcagtcc gtgtcactat tccactcagg ataccaccgt cgaggattag aattgcagtt
     1981 gtatcgtttt ctatcatctt atcggttacc gcctgaaccg aatcgtctac cgatgctgtc
     2041 acaaagaaac ggtccatcac ttcaattatt cttctcgctg ccagtccgtg tctaatggct
     2101 ttttcggtat tcctgtattt ggctatacct acaagctttt cctgatcaac taccggcaga
     2161 gtgtgatgac ccgttctctg catgattttg aagacatctt caaccttcat ctctgcaaga
     2221 gcggttctga ccggtgaaga cataatatct cttactttaa tgaggcgttc aatgttgttt
     2281 tcgaagtttt ttatgatttt ttcttcggct tctgctgacg tgcagttttc aaagtaacca
     2341 agtgtgtatg atccgccgct gtttctttgc acaccatcga gctcggcagt gtcgagattc
     2401 atgtatttcg atcttagagt gtaatatact cgattgttgg tttcgagaag gacaactaga
     2461 ttctctacgc caagaaagga ccagagtttt tctaccacga ggctaagccc tcccggcaga
     2521 aagtctcttc ttgcagatgc gatgttgatc tctacccctc tgatcgatgt ccttctcaaa
     2581 cccagtatca gtgattctat gagcctctgt tgtgaagcgc tcagcttgta gcccgagtag
     2641 attccaagat cgtccggccg tgcgcctaga tcgataagcc atgcggcagt actcagatct
     2701 tgtgctctgg tagaggcatg agtgaaattt ccggtttccc tgtagattgc tgtcgcaaat
     2761 agggttgcct catttctgga tatttctttc ttctttttcc tcagttgatt gcacatgaga
     2821 gtggtgcaag ctccaagatc cttgaaaatt cctttttgtg cttcactcaa gcttgtcctg
     2881 tgatggtcgt aaaacttcac gtcggtattt ctttgaatag attgttgaag agaagcggga
     2941 attcttttta gatcagatgt atcaacgatt atcaaagagg agagattgga aatccgtatc
     3001 tcatttacgt aataaaatga aagcaacagg cttttagatg acagaaaacg tttcaatgcg
     3061 ggaaccagct gtcccccaaa cacaatcatg tggtcgggat atatcttctg cgcagcaaca
     3121 gcggatgcaa atgcatcgaa atcgggatta cgatgtgtcg ttattacctt caagattgct
     3181 ccacctcttc agtttctcgt aaagcgtact cttacttatc tggagtatcg aagccgcctt
     3241 tgttttgttt ccgttctgtc ggtctatcaa ccaactgatg tagcgtcttt ccagctcatc
     3301 tagggagacc tctctgaatc tgaaatcctg gtcatctgct tgctccactt caggaaaggc
     3361 aaaactggaa acaccttcaa agctgccgtt gactgcgagg attgataaaa gtctgttccg
     3421 cagctctcga atgtttccgg gccagtcata tgctttcatc ttattcatga aatcctctgg
     3481 aatcgtcggt ggcttcttca ttccaagctc ctcacagagc ctcggcaaaa agtgatggat
     3541 cagaatcggg atatcctcac gtttttctct tagcggatct atcttaatct tcacgacgtt
     3601 caatctgtag tagagatcac tcctgaagtt tcctttcgaa acttctacct caagatctct
     3661 atttgtggct gcgattactc gtagatcaag ctttcgggat gtggtgcccc caagcctctg
     3721 gctggaggat gtctcgagga ccctcagaag tttcacctgc attggaaggg gcatctcgga
     3781 tatttcgtca aggaaaagtg ttccgcggtg ggactcttct atcaaacccg gttttgaatt
     3841 catggcaccc gtgaaggagc ccttttcgta gccgaaaagc tcactctcaa aaaggttttc
     3901 cggtattgca ccacagttta tggggacaaa ctttcctcgt ccacttttct ggtgtataga
     3961 tctcgcaacc acttcctttc cagtgccgct ttctccctca agaagcaccg tgactttctt
     4021 tggtgcgatt gctgcgatca tttgcttcag ctcgagaata tttttgctct ttccgattat
     4081 ctctacggag ggttcagtag tcaccaagtc ggccagacta tccctttccc ttgaaacctc
     4141 cgagaactca attgccctct taacttcgag aagcgcatgg tttagatcaa agggcttctc
     4201 tagaaaattg aaagcgcctt ttttgactgc atctattgca gtgtttatgt ctccatgagc
     4261 ggagacaact atcaccggcg aactgagtgc ggcttcggaa attatgtcta accccgatcc
     4321 atcgggaagt ctgacgtcca gtatcaaaca atcgaagacc tcttcagaga tctgcttcag
     4381 cgtctgctcg agattgtaga cctgaactac ttcgtatccc tcttcttcta gcgcagcgcc
     4441 caggagaccg ttaaaggcca gatcatcatc aaccacaagg actttgcgct tcatctgatt
     4501 gcctcctgaa ggttaataca aatactgttc ctttcccttt tacacttctc attttcaatc
     4561 ttacatcgtt ttcctcgcag aaattgtgga ctaggtacag tccaagtccc gatccctcgg
     4621 tcttagttga aaagaagggc ttaaatactt tctgcaaatt ggacgggtcg attccctttc
     4681 ccgtatcggc aacgatcagt gtcaagtatt cttttgaggc tctaactttt gcgataatgg
     4741 aggtttctga aggcagaccg gcttcgacgg cattcatgaa aaggttcttg ataacattct
     4801 caagccttcc gcgctgcgcc aagaccagcc ctttttcgag cgagattatt ctaaaatcta
     4861 tctttctttc ctgaaagatc gaggacatga aggtcgcgac tttcataacc tgctcagaaa
     4921 gatcgaccac gtcgaattcc tctccatacc tgaaaatctg taggattcca cctaccctac
     4981 cttgaaaggc atttagctcc ttttcaagtg tatcaatgta ctctggtttt cccgttctcc
     5041 tgtaaagatc aagcgcaagc ctaacatttg ccagcggttg cttgagacca tgcgcgagat
     5101 cggctactgt ttgcgcatcg acagcatatc ttctcgtttt ttccagaagc tgttttgtct
     5161 tccattgact ggtcatatcg gtaagggtta gcagataacc gcttttctgc ttatggacaa
     5221 ccgacacaag gtatcgcttg ccgagtttct tgctatagac ttcatactga ccttcttgaa
     5281 gcggaaaaat caaacctgtc cttcttgcaa atctatctat ccggagatat cttccttgct
     5341 gactaccgta agagaagctc tttgtgaacg cgtcgttgac gagatcaaaa tttccgttct
     5401 cgtccactga agcaacggaa atctccaggg tgttcagaag ctctgaataa tccctgtaca
     5461 gtctttcgta ataatttctt tcctcttcaa tttgaaatga aagacttctg tagaagccgt
     5521 aaatctgatt gaactgccct tcaagttcac cgattgattt tctctcgcct gtttgtacaa
     5581 gcctaactag cctctccaac tgtcttacca gagcaaaata gacggatgct aacgccgcaa
     5641 gagaaagaag agcaatcaaa accactcgat caagactgaa cccttcaatg aaagaataga
     5701 taagcccagt tcccagcacc acaccaccaa caatgtaatg gtaaccgggt ttgaaaaggc
     5761 ctccgtcaag ttttgacaat tgttatgggt tccccttcct caatggcgag gagctcccgg
     5821 gcacttccct tgttcacggt gatttctagg aagccgcttg aatcgaaatg ggcaagcaaa
     5881 tttccgggat tcacatcgta ataagtattt ccgaatgtag ctttgaaagg ttttctgccc
     5941 tttccaattt caagaatttc tccttcttca aaatcattag cgagagcctc tggaacattc
     6001 gtttccaggt taccaaaatt atcgtagaag gcaacctccc cagagagtac atttccttct
     6061 ctggcaggtt tcttgtattt aagatattcg aagctcatta gtctagaacc gagtttagcc
     6121 ggttctacgc ctgcatcgac atgggctgca acgggcgcga aaatgtctct tccatgaaag
     6181 ctccgggaat ttcccctgtg gtactctcta ttttccagct ctcgtatttc gtggattccg
     6241 tactcttcgg cagcaagtgt gaacaaacca ttgtccggtc caacaaggcg tcttccatct
     6301 ttcaaaacca tcattattgg ctttcttgat gtccctaccc caggatcaac gactgctaga
     6361 aagactgcat ctgccggaag atcctttatt gccctttgca agacataagc accgtgtctt
     6421 atgctgtaag gtctaatatc atgacatata tctattattt ccaccattct atttatttct
     6481 ctcatcacga ctttagtcac gccgacatag tagctgtttg atccccagtc agtaagaaaa
     6541 gcaatcattc taatccctcc atttgccaac aattgaagaa aacacttttt cgatacaacc
     6601 gaatccgacc ctgtatagat tcgaatctgt tttacagtca gaagcaccga agccaggaac
     6661 agcaatacag tagtttgaga tcagatttga aagttcctct tcgttacttg tctttgccgg
     6721 aagaagcaga accgcaagaa cttcagcctt ctctctaagg aaatctatat gccagtgctt
     6781 cttcttttct tctaagaggt gcctcttaac cctttccgtc agtgagttca ttgccgatcc
     6841 aacatataag tagtatcccg attcaagatg ccactttttt ttgctcgaag atacctctgc
     6901 cgcttctctg agaaaaagca agacaatgta atcaccctta ttgtaatcca taatgtagat
     6961 attatacgac agcaaacctg ccaagaacca tttgctctga aatgaaaagg tgtacaatgt
     7021 ataatttgtt gaggtgattt ccttggcaaa agttgtggtt aataccgtga gcataggggc
     7081 caatgcgaaa agagttagag aactggccga agttaatggg gtagaaatcg cagcggttac
     7141 gaaagttgtg tgtggagacc cgacgatagc atctatactg ttgaatagtg gagtaaagga
     7201 aatcggcgaa tccaggttgg aaaacatttc cagaattatg aatgctggaa ttgattctga
     7261 ttttcttttg ttaagacttc ccgagagatc aaaattcaga gaagtgttgg ataatgtaga
     7321 caccgtgctc attggagatc ttgattcatt gagcaagctt tatgaactct catctgaaac
     7381 agggcaagaa gtgaatttca tatacatgat cgatacggga gaccttagag agggagttat
     7441 gtattacgag gcggaatcgg ttttggagaa agcattcgaa attgccggtg aaaaccttga
     7501 aggaattgga accaatcttg gatgctttgg gggcgtaatt gcaactccaa agaaattcga
     7561 gattcttctt tctttaggtg agttccttag aaaaaagact ggctattcat tgagaagata
     7621 ctctgccggg aatactgcgt cactgcctct tctcgaaaaa gggatagttc cgaaaggtat
     7681 aaaccatttc agattgggag agtcgattct ttgtgggacc gatgttacga acaacagaga
     7741 ggttcctgga acactgcagg atacgtttac actgtatggg gaagtaatcg aaatagcgga
     7801 aaagccatcc gtaccgatag gcgaaatagg tcgtgatgct tttggaagaa tcccccattt
     7861 tgaagacaag gggaagagaa taaaggcgat actggatctt ggagaacagg atgttcttcc
     7921 ctcaggattg acgccacttg tcgggggatg tgaagtgcta cacgcttcga gtgatcacct
     7981 gatagtcgac gtgaccgagt ccgaaaagtc tttttctgta ggggattcga ttggcttcag
     8041 aatgtcgtac ggtgcgctgc taagagtgat gacctcaccg tatataagga aggtttacga
     8101 atgataaggc gccttcaaaa gtgtgatgga gaaagagtaa gagagatcgt aaagacgatc
     8161 tgggagggtg atgactacat tcctcatgtc ttcgaaaaat gggttgaaga cccttcatgc
     8221 cacttcatgg gactctggaa agacgggaca ttagtaggaa tagataatct cagactctta
     8281 agcgataaag tgggctggat ggaaggaatg agaatagatc ctgctcttca gggcaaaggg
     8341 tttggcaagg aactcggaaa acaaaccctg gccattgcga agagaatcgg gctcgaaagg
     8401 ctttactttg cgacctattt cgacaatact gcctcgatca gaatgaatga agccttcggt
     8461 ttcaaccgca taggtgtttt cacaaacctt gaaagtgaga taggagatat ctcagattct
     8521 tccttcgctt ttaatgtgga ttcagagatt cctgaaattc tcgatcatgt aagtgaagac
     8581 tgggcttttc tccctaagga aattccagac aaagcgagat tcctaattca gccaactagg
     8641 attcacgatg gggataactg ggcagttctt tccaaaaatt cgaaatctga aagctgcgtg
     8701 gacatcaatt acatctcaat agtggaagaa cggaaagcca ggtccttcgt gcatgggctg
     8761 aagtgctatg cacagtcgaa agggtttgaa cgggtccata caatggtgag ggaggacctc
     8821 gatttggagc ctttcttgta caatggattc aaacctttcg aaagagtaag ggatgtcttc
     8881 ctttattttg cggacagctc ggttcttgag atttgactcc cagcagaaat ggcttgaatg
     8941 aatccgttgg tcggtattac tcttgattct aatatatcta cgtcggtcgc tcctacacat
     9001 cgatgtctct atattaagca ttacgcaaac aagagatgat taacgacagt tctatgcaaa
     9061 tgaactcttg gctatgatcc gaatttcttg tctaaatcgt gaagttcaaa ggttatgaat
     9121 cactaatttg gaaggacatt acatggatgt gaggcaggca caattgagaa ttcatagtca
     9181 tctctcgtat aatttccctt taaccttcat cgatacgatt gtgcacctca gaaagaggaa
     9241 ctgcattctt tgtaaaggcg gacaggaaag ttgagagtga caagcgaatt cggttcattg
     9301 ccctgcccgt gtgtcttctg agttagtgca aagtgaaaaa acatgaaaga cacctcccta
     9361 tacttgggga ctttgtccga agcaagagac tgcgtttttg tgagctcgag agactactaa
     9421 gaaactaagt acctccctat ttccttttct cttacattct aagtcactct tggccgggtc
     9481 tagagccgat tgggtttcct tcgttgagtc atagtctcaa gaaatcagag cggatttgta
     9541 cttgagaatc ggcctatcga gatcctcggc agtctaacac gaaagacttt gtgattacct
     9601 ggggttgtat tatggcttaa ggcagaagat cgtatcggaa atcagatcaa cctttgacag
     9661 aaccagcaag aagccctctc agaaagtact ttccgagtaa tatgtagact atcaatgttg
     9721 ggagagcggc cagcagcgcg ccggccatct ggacattcca ctcgacaacc tgactccccg
     9781 ctagattcac aagggcaacg gtaatcggct gttttgtggg gtcgctggta acggtcacag
     9841 cgaaaagaaa ctcattccag atattcgtga attgccaaat gactactact acaaaaccag
     9901 ggagggatat aggaaagaga accttcagat atgtcttgaa tatgcctgct ccatctatgt
     9961 tggccgcttc gattaattcc gtaggcactt cgctgtaata gttcctgaaa atcaaagtgg
    10021 ttatcggcag accgtagacc acgtgaacca ataccaatcc ccaaattgaa ccgtaaagtc
    10081 caatatcctg gaggaagcga atcagaggaa aaagaacact ctgataagga ataaacattc
    10141 cgaaaagaac gagtgcaaag agaagatctg agcccctgaa cttcagttta gacaaaacat
    10201 atccgtttat cgatccaacg attgccgaaa gaagcgtcgc agggatgacc agcagaaacg
    10261 agtttcttaa gttgggtgca aggcgactga aggccttggc aaaaccatcg aaggaaaggt
    10321 cgttcggagg aaaccacatt cgctcaagac ccacttcttt caggggtttg agacttgttg
    10381 ccaataacac atagatcggc atcagaaaga ataacgcaaa aaccgtcaga agggcataga
    10441 ttagaatcct cttgatcata ttctcttctc ctttctgaag gcagaagcaa ggtaaggaat
    10501 tatgaccaca gcaaccatca gaagcatgat tatcgctatt gctgagccag tagcatattt
    10561 gttcgctctg aaagtggttt cgaacatgta gatggcaggc acgtcggtca cgttgtttgg
    10621 accactgccg gtcatagcgt aaacaagatc aaagatcttc agtgagatgt ggcctagaat
    10681 aatcattgcg cttaaagtta tcggtctcag aatcggaaac ttgatcttcc agaagatctg
    10741 ccagccggtc gcgccatcaa cttcggcagc ctctaccaga tcctgggata ttccacgaag
    10801 ccccgcaagg tacatggcca ttgtgtaacc tgacatctgc catacggctg cgattatcac
    10861 cggaatcagt gcaaagttga aattgaggaa actctcagtg ctggtgaacc acctccattg
    10921 aagaaaatcc agccccacca gactgaatag aaggttcata ccctgaggat tgcttggtaa
    10981 gattcccgga gcgaagatcc atctccagac tgcgcctgtc acaacaaatg aaagcgccat
    11041 gggaaacagg tatatatttc taaaaatgcc tgatcccttg aggttacgat caatcagtac
    11101 cgccaaaaca atcccaagca gaagacaacc gagaataaaa aaaagggtga aatacagagt
    11161 gttccacaga tcggtctgaa acctcggatc ttcgaaaagt ctgaaataat tcctcaatcc
    11221 aacaaattcg aattcgcctt taagaagcct agcaaagcta ttccagttgg aaacagaagt
    11281 tctaccagtc caaccaatga agaaataaac gaaaactcct attgccgcaa aggtcggaga
    11341 gagaatgaga aaggaaataa tccctcgttt tgtcttcctt ttgatatgga acacccccaa
    11401 gaatggggcc tgcagatgca ggccccactt cgcaaggatt aatagatgta atcttcagca
    11461 gcgcttttta tggcactcag aaaagctgct ctgtcttttg tagtaatgaa gatgtttact
    11521 gcatcgttca gctccgttat aaagccttca ggcgcagcag atccatgtgc tatagaagga
    11581 caaagagcgt ttgtagaaaa gtcctccata gaccacgtca ggtaaggatc gtagagtgat
    11641 ttgtctgcat ccagtcttgc aggaatcgaa ccctttatcg ggttgaaggc atcttgacct
    11701 tcgactgagg caactatttc gagccactta agagcgtttt ctctgtttgg tgcgcccttt
    11761 ggcaagccaa atgtatccgt taccaccatg aaggagcctt cggttcctgg aactgccatc
    11821 cagctgaact ccacgcccgg agtccatcct agagtcttca aatatccttc ggcccagtct
    11881 cccatgacgt tgaaggcagc ctttccatcg aagaccatcc ttgttgcatc ctgccaggtg
    11941 agagctgcat ggtcttcgtt tacatactta atcagttctt cgaaaattac tatggccctt
    12001 tcaattcctg gatcttcgaa acttgttgtt ccatcccaca agccattgta cttatctggt
    12061 cccagagccg aaagcaacac ggtctcaaaa agatgaccgg cctcccactt gtctttatcg
    12121 cctaaagaaa gtggtataaa gcctgcctcc ttgatttttt cacaaacttc gagaaaaccc
    12181 ggtccgtcgc ttggaacctc atcgattcca accttctcga gaatctcgtt gttaataaag
    12241 acaacatttc ccctgtgcac attcacggga attgagtaaa cttcaccgtc gtaactgcag
    12301 atggtcataa tatctttcgg gaatttatca agtattcccc agctttctaa gatgtcggtc
    12361 aatggctcca tcattcccgt aattacgtag gtgtcgatta gttccattcc tccgtggacc
    12421 tggaatgagt caggaggatt gccgccaagc atgcgtgtct tcaaaactgc ctttgcgttt
    12481 gtgcctgcgc ctcccgcgac cgtcgcgttg attatctcca cgtcggggta ctttgctttg
    12541 aagacctcat aaatggcagc gagtccttct tcttcgcctc cacccgtcca ccagctaaag
    12601 atctccacac ttccggaagc aaagagcaga ctcacggaca aaagaagaac tgcaagcaaa
    12661 ctttttctca tttaaacacc ccctgttatt cggcatacag ccgtacggtt atcttcttcg
    12721 cctttcagac caaactagcc tctacttgag attatactac cgttaacaat ataataagca
    12781 acttcaaaaa aggggagtaa agattacaga ttgaactgtt cctcctaaat aattgctcga
    12841 acgtgtgact gagttcaaca caaagaagat tgatcgcatt tcggaacctg gaacgaattt
    12901 ttgaatcgga ggttttctct gccaagactt ataatggtat tatttagtga atagagacaa
    12961 ggagggatta ctttgtcatc agtgaatatt tccattatcg gtgcaggaag tgctgtgttt
    13021 tctctcagat tggtgagcga tctttgtaaa acggagggcc tttatggcag taaggtcaca
    13081 ttgatggata taaaccaaga tagacttgat gccgtttaca ttcttgcatc gaggtttgca
    13141 gaagagatga atgccaatct cgagttttca aagactactg tgctggaaga agcaatcaaa
    13201 gggagagact tcgttgtaaa cagcgctttg attggaggac atgcttttct ggatagggtt
    13261 agagcgatcg gcgaaaagca cggttatttc agaggcatag attcacagga attcaacatg
    13321 gtatccgatt actatactct tacaaactgg aatcaactct cgtttttcct gaaaatcgcg
    13381 aagcttatgg aaagacaggc acctgacgcc tggctacttc aggctgcaaa tcctgtcttc
    13441 gaaggaacta cgctaatcag aagacattct agtattaaga tggttggctt ttgtcacgga
    13501 cattacgatg tccgtgaagt agcagaaacc ttgggcatac caatagagga agtagactgg
    13561 caggttgccg gtgtgaacca tggaatctgg cttaacagat tcaaaagaaa cggtgaggat
    13621 ctctacgctt tgattcaaaa gtatgcagct aattatgatc caaggagatt caaacctatc
    13681 aaccctttca atgttcagat gtctccggcg acaattgaca tgtacagatt ctatggattg
    13741 gttccaatag gagatacggt tcgtaacagc ggctggaaac accactacaa tcaagagact
    13801 ctcgtaaagt ggtatggggc tccatggggc agtcctgatt ccgaacccgg ctggcaatgg
    13861 tacaaagaac agcttggaca ggtgacaggt gctactgtgg cacttgccaa ggcaatcgag
    13921 cagttccccg aggcaagtct cgaaaaattg attgtcgaag gttcaagagg tctttcagga
    13981 gacttcgcca aacaagcggc tcagctttac aacaagaatt ctctcagcgg tgagcaacac
    14041 atacccttta tagacgcaat aataaacggc agttcagcga gatttgtctt aaacacgcta
    14101 aacgaaggag caattcccgg gatcgaagat aacgttgccg tcgaagtgcc tgctttggtt
    14161 gacagagagg gaatccatac tgaagccata gatcccccta ttcccgaaag aatcgttaaa
    14221 tggtatttga agccaagggt actaagaatg gagtgggctc tcgaagcgtt cattgaaagg
    14281 gattccgatc taattgttga aatccttttg aaggatccaa ggacaaagtc atatgagcag
    14341 gcgaaagcag tggtaaggga aatctttgag acggaagggc taaatgaata ggttcaaaga
    14401 accgatattt catgggtaaa tcgaagttaa attgatctct tctgtttgtt cccacaaggt
    14461 aatggcgtag gaatgcaccg aggcttccaa attcgatggt tgttaaactg acggggtggt
    14521 cgaattgaat gaggtcttcc gttgtccctg ggctgaagtt gacaaattgt atattgagta
    14581 ccacgatact gaatggggtg ttccactgca cgatgacaac atatggttcg agtttctgat
    14641 tctagaagga gcacaggccg gcctcagctg gcatactgtc ctgaagaaga gagaagaata
    14701 tagaaaggcc ttctctgggt ttgatcctcg agctgtttca aagtatggag agaatgaaat
    14761 aactaagcta gtcgaaaatc cgggaataat cagaaataga aagaagattc attcggcaat
    14821 taacaacgca aagagattta tccagattca agaggagtac ggaacgtttg acagctatgt
    14881 gtggagcttc gtcaacaata agccgatcat aaacacatgg gaaagccttt ccgagattcc
    14941 ctctgttact gagaagtcag ttgaaatctc gcgatccctt agagataagg gattcacttt
    15001 tgttgggcca aagatagtat atgcgctgat ggaggcaaca ggcatggtga acgatcatct
    15061 ggtgggttgc ttcagatatt ctgaagttct ccgatgcaat tgagtgttcc aggatacact
    15121 gaagaattga tgatataata actcttaact tgtaggagat taatgggggg tttttatgag
    15181 ctggcttaat acagaagaga tttttgccct catagatttg atcgattcca gtaacgattc
    15241 atatgaagag agattcaaga gagttttagc cactcctctc gccagtatgt ttaaacaatt
    15301 ccatgtacgc gattcagagg caagggaatg cctggtggct gccttttccc gtagagaaga
    15361 tctttccggt ctttcaaagg actgcacgga gctcatagcg tacatggata aaattaggga
    15421 aagaaagctg gaactgagag ctttcttcga tttgattaat gagaatgaag caggaattag
    15481 atctaaggca atatccaaag cacaagagta tcttcctaag ggagtaactt tcgatggcct
    15541 aaaggtcttt ttcattccta tgccttataa tgcaaatgcc gatcatagag gtgtgtattt
    15601 cgatccgctt tttgctcttg atatgggtct tgaaggtatt gaagaagtaa tggctcatga
    15661 ggcacatcat gtagcaagga attcgataac aaaggaaaga cttgagttcg gtgattctcc
    15721 acttgatcag ttggtctaca gatttgtgag catagaatgt gaaggaatcg caaatatggt
    15781 tagcgatgcg tcgagaatac catcaatgaa gagaattgcg ctgcagcgaa cgaaaatgat
    15841 gggagagttc gaaaagcatc tcgaactgct tcaagaagtg ttcctgaatc tctcctttaa
    15901 gaacatctcc gaagacgaag caaggtcgat tatggaaaga agctggttta gtgtcgggag
    15961 ccttgccccc gtgggaatga gaatggcttt ggagatcgaa aatgagttag gnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncatcttcta ccatttttgt cagaacatcc
    16141 acagactctc cactccatcc ataagtacca aatacggctg cctttttgct cttgaaggac
    16201 agacctttta cttcatgaag cataccggaa agcggtgcca agattccttt gttcactgta
    16261 ggagaaccaa agagaactgc cttggcctta aacaactccg tcacaatatc gttcttgtct
    16321 atttttcccg cattgaagag tttgacggcg gtactaccac tagcgctggc aatgccttct
    16381 gcgatggctt cggccatttt tctagtcccc tcccacatcg tgtcatatac aattgtcacc
    16441 tgatcttcct ggtagttgtc tgcccactca agatacttct tgacaatctg tgcgggatcc
    16501 tcccgccaga taactccatg actggggcaa attatgttca atggaagatt gaatgacaag
    16561 acctcctcaa tcttctttat gaccagtctg ctaaatggag tgaggatatt tgcgtagtac
    16621 ttcactgctt cttgaaatag ttccgcctga tctacgaggt cgttgtagag aagttcagtt
    16681 gcataatgct gcccaaacgc atcgttgctg aagagaattg cgtcaccatc gagatatgaa
    16741 aacatcgagt cgggccagtg cagcataggt gcttcaataa aggtgagcac cttactgccg
    16801 agatttagag tgtcgccggt tcttactgtg acgaaattcc agtccttgtg aaactgccct
    16861 ttgagggact tgactgcgtt tgcagtacaa tagatcggcg tgccgggaat ctctttcatc
    16921 aacgcaacca gtccaccgct gtgatcaatc tccccgtggt tggcgattac gtagtcgatc
    16981 tcggatagat caatttcttt tttcaggttc tccacgaatt cctctgcaaa aggtgcccag
    17041 acagtatcga tcaaagctgt cttttcatct ctaatcaaat atgcgttgta gctggatcct
    17101 ctgtgggttg aatagtcttc tccatgaaat tttttcagtt cccagtcaac ctttcctacc
    17161 catgttacgc tatcggttat ttttctgccc atttctatac ctcccttaaa tacatcaata
    17221 ctctaccaag attaccggct ttaagcggta acatttgtta cacaattctt caggttctga
    17281 ccaaaaatca tctgccgtgc aatgtttcta attaaaacgg agacgtctat cagcttggga
    17341 aggctcgtta ttgaggcgtt tgatcatgac atttcgcctt ttcttcaaga gatctgaatt
    17401 ttgtgtgctc aatggttggg atctaaaagt atcctcagat gcgtgatatg ctaatatata
    17461 gtttgacgca ttgttttttg ccctttttcg ttctggttgt tgagggttca gcaaagatta
    17521 gtcgcaaatg attggagtat ctcatgagta atgacatcac ttatttattt gatcttgatg
    17581 gaacgcttag ctcagtttcc gatgaggatt ttgcacggcg gtattttcag ctcatctcgc
    17641 tctttgccaa tggaaaagta gactttgaaa aactgatggt ctctctgaaa agtgcactcg
    17701 aagtgctatt tgatagaaga gacggcataa agagcaatta cgaactcttt atggaaagct
    17761 ttgtccattt catgggagac catacggtaa aatggtacga ggatttcttc gatgagtttt
    17821 acgagaacga gtatgaagaa cttgagaaaa ttgtgagccc tagagagaat gtggtggagg
    17881 ccctgaaaaa gctttacggc tcgggtagaa agataattat tgcaacgaat ccagtctttc
    17941 cacacaaggc aataagaaaa agacttcaat gggtgggagt ggatgaaaag ctaaccaact
    18001 acgtgaccac tatggagaac tctcattatg taaagcctag tacagaatat tacctggaaa
    18061 ttctggagaa tagctcactc gatgcaaagt actgcgtaat gatcgggaac gattacaaaa
    18121 tggatggtgc ttgcacgtct gcggggattg aatatgtcga tgtgagttcg attcaatcta
    18181 tctggagaac aacttagact gcactcccgc cataagttca gccgtaaatc tcgagatcaa
    18241 gcgcagaaca tcttctctgt cctgggcatt ttcaacgaaa tcatatttat caccatcgat
    18301 tatcaacttc cgacaaaacg tgactctgcc aatccattct tcgtatagtc cgttgagcct
    18361 ttcgatataa tcctcgctca cgccgctttc aaaaccccgg cccctctttt ttatcctttt
    18421 cagcaagacg ttcttagtgc attttatgta gacaatacca tcgggcgctg ttaagtgttc
    18481 tgagagcgtt tcataagtgt ccagataaag tctccagtcg cggtcatcta tgtagcccat
    18541 tagtttgaga ttgcgggcaa atatctcaac gtcctcgtaa atagtcctgt cctgaagtat
    18601 gacagcacct tcagttacga ctctcttcag aaagtcaaaa cgctttatta gaaagaacaa
    18661 ctgcgagtga aaggcccacc gctccatatt tgaatagaaa tcctccaaaa atggattttc
    18721 atccacggcc tcgtagacac ctctaaagcc cagtgtttcc tcaagcaatg aagtcagcga
    18781 tgattttcct gcaccaatgt ttccgcatat tcccaaaatc ataagctcgc ctcgattctg
    18841 ctgaaaacca tgttgagatc atcaacgttt cgcacaaagt ctatgcttga agtatctatc
    18901 ctcaggactc tgctctcgga aaacgctgcc atgtatttct catatgtgtc gcttagcata
    18961 gaaatatacg ttctatccat tctcctctca aacggtctat ctctgagagc tattcttttc
    19021 atcagggtgt cgatatccgc gtaaagatag attatcagat ctggataaag cacctgatct
    19081 ttgagaacgg aaaagatctt ctcgtaaagt ttcaactgtt cgcctttcaa agttagagac
    19141 gcaaaaagat gatctttcat gaatgcatag tctgagataa ccccttgtcc ctgcgaagcg
    19201 gctttcttaa catttgattg ctgatcgaat cggcttatca aaaagaaaag ctgagtttga
    19261 aaagcccatc tttccatgtc ttcatagaag tgtgcaagaa atgggttttc ttcaacaact
    19321 tcgagcacta ccggcattgt gtacttttga gacaacaatt tcactagagt cgtcttacct
    19381 actccaatta caccctctac ggaaatatac ttcccgcttg ctttagaaaa catcataaaa
    19441 gtttccttcc cccgtttctc aaaacatgac caatactctc aaccacctga ttgacatgtc
    19501 cgcgatgcct ttgcaaaagg aagaccgttg gaaaaacagc tgacattcca tgactcagtt
    19561 attcctaaga tgtcctaagc ccatcgaaaa agggcttaag ataatcgatg acggattcaa
    19621 gatagtaatg tacagtttca tggtaacccg gaacagagag tactatcgaa tcaccgattg
    19681 ttccggctcc aactctgtaa agacacaacg agggatcggc gactattgcg tttctaagca
    19741 tcgcagtttc aattcccgtt gctcttttgt ccacgagaga tagaacgacc tccggaccta
    19801 tgtcctcttt gtatagcccc gttcctccaa gaacaagaac gatgtcgttt gagcatgaat
    19861 ctgagtatat agcctctttc aggtcgctca ttacagggtc aacgtctgtg gatccagtat
    19921 acgtgatgcc gtgactctcg aggatcttct tagaaatctc tatggattgc gatatctcgt
    19981 caaaaaagcc agattcaaga accctaatta ttttgcagct gtacattgaa cacctccgaa
    20041 ctacttgaag aagacataag tagaaattgg ttcgagcttg accttacctg agagtctttc
    20101 gaggggcttc tcagagattc tatctctatc caagacgagg ttccagcttt catcaggtag
    20161 agttatctcg gctggttcaa ggttcccatt gaatatcaca agtatttctt cccatgagtc
    20221 accagctgac ttgccgtcta tcacgtacgc aacgatattt ctgggagcat caagaaattt
    20281 tagattcttt cttatctctt cagccgtact catcctgaat gatggatggt tctttctaag
    20341 ttctatcagt cccttataat aggtgaagag gtcaatgaat tctgccttcc ttgaatagtc
    20401 catcttgttc aaatctacac cggcattgta gctgttatca ttgccgaact tcgttcgagc
    20461 aaagtcaact ccaccgtgaa gaaatggaat tccctgagaa agaatcacta tggcatttga
    20521 tagcttctgc gcgcttgtca gcaactctct tgaagcatct ggcattgctg cgctattctt
    20581 gtcccacaga gtctgattat cgtgagcaga aacatagttt atagtctcag aagggtcatc
    20641 cgcaaagtcc ttaatgtcac tgctgtattc gatacttccc acgatgccac gcataatcct
    20701 tctttctttg gccctgctcc caagagaaaa gccctttact ttctcatcga aaacgcttcc
    20761 tcttattgca tctctgagat tgtcgttaaa aacagctaca ccggttccac gttgatcgcc
    20821 tttcccgaat gtaacggttg ccccccaacc tccccacggc tcaccataaa gcagaatcgt
    20881 cttatctatt gcatgaagtt ctcggtcgat ggccagaacg gtttctttat ctataagacc
    20941 catcaaatcg aatctgaagc catcaattcc gtattcgttg acccacatct taagagaatc
    21001 aagaatatgc tttctcatca tcggcctttc tgaagctatc tcattgccga ctccagttcc
    21061 gttcaaatac gcacctgtgg cgtctgttct atagtaataa tatgggactg tctgatcgaa
    21121 gggagaagaa gaacctgttg tagccgtatg attgtacaca gtgtcgagaa ttacccttat
    21181 tcctttttca tgaaatgcca tgaccattgc cttagattcg agaattctac tgagaggact
    21241 gtaggggtca gtactgtaat gtccttccgg aaccatgtac agataagggt catagcccca
    21301 gccgtattcg ccttcctcac cttcatttac gtagtgaata tcgttgaaag gcattatgtg
    21361 tacgtgtgtt acaccgagct caacaatgtg gtcaattccg acagtaactc catcagttcc
    21421 cgttcttccg gtctcggtta ggcccaagta tttaccctta ttctctacat tggtatttgg
    21481 gtcggaagtc atgtctccaa tgtggatttc gtatattatt gcgtcctccg gtttgttcaa
    21541 catcgggact ttttgttggg cccagccttc aataatggtt ttagcgggat caacgacaac
    21601 tgatctctca ctgttttttg taactgcacg ggaatagatg tcaactgttt ctctttcctt
    21661 gccataagag acaaatctgt atttgtaagc ccgcagatga agattgcctt gaacgattgc
    21721 ttcccaaaca cctgcttcta ttcttttcat ggggacttca ataaagctgt ccgagtagag
    21781 atcgtcaaag agaattagtg ttgctgaaga ggatacggga gaccagactc tgaagactgt
    21841 ctccttctca gagtaaatcg aaccaagttc gccctggtag aagaattcag aattatctag
    21901 cgcttttctg acaattatgt cgcctggaag aaaaccttcg atctccagag atagcctttt
    21961 gctaactgac ttgggcgcaa gtggttcggt cagtgttatc ttcacgtagt tagttaccga
    22021 tacatcagtg ggatctgcct tttcgaagct ttgaacctta accggcgaac cgtctagaag
    22081 gacttcaggt aagactgtct tagtatcaac cggtgctgaa agtgaagcgt agatctcact
    22141 caaagaatca agaaaggcca tcttaactct gggagttaga tcaattctgt ccgggctcgt
    22201 caaaaaatcc tcttcccctt cgatcaccca tatttcagca actccagaat cgggaatatc
    22261 tacgaaccta tctgctgtga tatccttctt ttcccattct cttagcctta ctatgaatcc
    22321 cagtctagaa tgtttttcag agaactttac agttgtaact actccgaatt cgtctctcct
    22381 atcaaaagag taagacttac catccatact tttcggtttg tcaggccaga cccataggtt
    22441 ccagccatcg tagttgtcat cgtacctgtg ataatggatt ataattacag tcgctgcgtc
    22501 ataatcttct gcactttttc ccatactttc tcccgtaaaa gcaaaaatcg ttgttccgaa
    22561 gattagcaaa aggaagaaca acactctttt cattataaag cctccctcaa tcaagtgggg
    22621 ttacggtgaa tccactaatt ctcctgtcat attcatattc gaataacctc tctcttccat
    22681 tttcatcctt aatcttcaga taaccgcctc ggggatccag ataactttcc cacaaaaaat
    22741 cgtattcaaa cgaagtggtt cttgatctat ccagtgtgat aagtattcta agctcttcag
    22801 cagtttcaat agggtcttga gaatccaacg aaaaaacatc ttcgtcggta gttcgatctg
    22861 ctcgagataa gattgaaacc tctgaacctt caagatatcc gcccttatcg gcagcaagag
    22921 tcagaagagc gtgctcaccg ttatcgaaaa tatcaatctt agaaatcgtt aatcttgctg
    22981 cacctcttga atcttcctct acaacttcct ctatcgcttc tctttgactt gtttcttcta
    23041 tgcccgattc aagaacttcc ggttcttcag cccagattcc aagacttctt tcgcgggcaa
    23101 attcttcggc cggaacaagt tcgatttctc ctccgtcaac tgctttagat agaccgctag
    23161 atacgattat agattgaata agataagcct caccatccag aaggatccag acgtaagctg
    23221 aagcttcctt agatgtggtc acttcatgaa caatcgcttc aagtcctatt gtgtttctta
    23281 ttagattcaa gcacttcttg ctgttagagt ctgtagagtc atattgagga atctctactc
    23341 ctgacagcga atatcttttg agatggctat tggcaatgat caagagatca taattgtttt
    23401 ctgtccccac gattattccg gtaaaccctg aaaccgatgc ttcgggcgtg acataataaa
    23461 atccttccat aatctgggag ttagcaaatg tgacttcaga gtgcgggaga gctatgaaga
    23521 ctattaccat taaaagatac agagacctca aaggcttgtt atcctccaag tagcacacct
    23581 ccaggcagaa ctcaaaaaaa gtataacatt atcacccgac tttcaatact ctatggtttg
    23641 agcaaatgct gtatgctgtt caaatcgttt ggttttgctg gagaaactca acctttcgaa
    23701 agcgttctaa gcctgactat gaaggtgaag ataacaacgg gaaccgtcca acccaaataa
    23761 ttgaaaacca tatctagaat atcgaactgc ctgttgggag atagaacctg aagaaattcg
    23821 agaatgacag gtcctacaat gagaaataaa aagaaggttt tcctaaacgc cttgctgaat
    23881 tcttctcttg aaagaataac aaaaaggaac cctcctgcaa agaaacccac aaaatgaaga
    23941 aacttgtcgc ttcccacttc gattgccgga actaccggtt tcaggtagaa gtagacggtg
    24001 aaaactatgt aaattgctgc agcaagagcc attatcaatc ttctcatcaa taagaattat
    24061 gctaaccatg cgcactaatg tcaatgacgc gcaccgaaaa tccctttgag tctcgagttg
    24121 aaaagcttct atttctttac tataatgaag tgtattgtaa atcgagagga ggacacaatt
    24181 catgtccaag gtttgtgaaa tatgcggtaa gtcacctact acagggaata tggtttcgca
    24241 ctcaaataaa aaggccaagc gctggtggaa gcccaacgtt cataaagtcc gcgcagtcgt
    24301 tgatggcgag gtaaagcgaa tcagagtttg tgcgaagtgt ctcaaagctg gaaaagttac
    24361 aagagccatc tagccataac gcgctattcg cgctttctta ctgagaaggt atgaaatgaa
    24421 tagcagaaaa accttcgctt tgatcgatct atctgcatat agacaaaatc ttgtttatct
    24481 gtccggaaag gcgtctccgg cgaaactgat ggttgttgtc aaagcagatg cttacggtca
    24541 tggtgccgtg gagctttcga aagttgctca ggaatcggga gtcgatcgct tagcagttgc
    24601 ttttcttgaa gagggaatta agatccgcca aagcggaatc tctttgccga ttcttgtgct
    24661 taactatgtg gagaaaaagg aaatccctac tgcaaaggag ttttctttaa cgctatctct
    24721 ttcttcattt gctcaacttg aagacattat tggcttaaat aagcggttac ctgatttcca
    24781 gctggttgta gatactggaa tgaggcgtct gggattggaa tgggaagagt cgattgagtt
    24841 gtataattcg gctgtcagaa agggaatcaa tataacaggt gcttatacgc acttcgctac
    24901 agcagatgag aaaggcagtg gcttcgttct tgagcagaga gaagcttttg gaagatttct
    24961 tcatggcttg ggacaaagac gaaacagaca tttcgaaact catatatcta atagcgcagg
    25021 caccatctat ttcgacaatt ctgattttag ttatgttaga gcgggaattg ccacatatgg
    25081 cctacaacct tcgaaggaaa ctgatgaaaa cctgaaacct attttgtcgt gggttagctg
    25141 tgtatccttt gtgaagagga tacgtgccgg ctattcggtt agttatggca ggacttttgt
    25201 tgccgatcgt gagatgcttg tagctacaat tcccgtaggc tatgccgatg gatacaatag
    25261 actgctctct aatagaggat atgttatcat ttcgggcaag agatgcaaag tacttggcag
    25321 ggtttgtatg gatcaattcg ttgttgacat atctcacatt gagaagcctg tctcgattgg
    25381 tgatgaagtt gtgatagttg gtactcaaga aggagagtcg ataaccgccg aggagatagc
    25441 aaatctgtgc ggcaccatta actatgaagt agtttgttct atctcttcca gagttccacg
    25501 ggtctataag aaaggagtaa attgatgaaa ctccgcgata tcattggaaa cgagatattg
    25561 aatcaaataa atgccgatca ggataagacg aacagggatg gttttccttt tgtttccgat
    25621 ctacgcccgg gaagtactgt gttatctgtt ttcaaggtcc attctaagag aattcaagag
    25681 gcccgagacg gaaagaagtt tcttctatta acactttccg ataagacggg agctatacga
    25741 gcaatcgatt ggtttaatgc agaacgcaat gattccactc tggaacaggg tatggtagta
    25801 agaatctccg gtcgagtagc tgtctatgaa gatagaatcc agcttaatct tgattcggaa
    25861 gggattcaga ttcttcagat tggacagtac gattatgaga gatttatggc tgtcacaacg
    25921 aaagatattc ctcggatgta cgatgatctt cttagtctcg ttggaagaat gagggatgac
    25981 tatattagag ctctgctcaa agatatcttc gagaacgaca agaagttcat cgagaagttc
    26041 attgtttctc cggccgcttc taaggttcac cacgcttata agggtggtct tctggagcac
    26101 acaatgtctg tcgccgagct atgcgaattc ttcgcgacga agtacggaga ctccgttgat
    26161 ggagatcttc tagttgcggg ggccttgtta catgatattg gtaaagtgta tgagtacacg
    26221 attactccga atggaattga gaggacaaat gacggcgaat tagtgggaca catcgctatg
    26281 ggcatagaga tggtaagcaa ggcaatatct aaaattcagg gcttcccccg ctatcttcag
    26341 actgagataa aacatctcct cttgtctcat cacggggaaa tggaatgggg ctcacccgta
    26401 attccaaaga cgacggaagc aattgttctt catatggctg acgatttgga ttcgaaggtt
    26461 gctcagttca gagagattga agaaagggaa ttcaatggct caactgcttc ctggagcaat
    26521 tacgatcgtt ttcttaacag aagagtgttc atgaaaaaca gaagatccgg cgactcaaat
    26581 agttaggagt gatactttgc cagacaagct tgtaatcgtt gaatcgccgg caaaggcgaa
    26641 aacgatcagt agatatttag ggaagaacta tgaagtcacc gcttccaaag ggcatgttag
    26701 agatctccca gaatctaaca tcggacttca tcctgaaact ttggaaccga cttatgagat
    26761 tcttaagggg aaggaaaagg tcgtacaaga gctttccaag aagtcaaagg gaaaacaggt
    26821 gcttctcgct tcagacctgg atcgtgaggg agaagccatt gcatggcaca tttccgaact
    26881 tctgggtctt ccacaaaatg aaaagaatag aatagtcttc aatgagataa cagaatcggc
    26941 aataaaggaa gcaatacttc atccgagaga gatagacatg tctaaagtcg aggctcaagt
    27001 agctagaagg gttctcgata gaattgtggg ctacaaaatc agtccaattt tgtggagaac
    27061 tatgaagaga gggctcagtg ccggaagggt ccagtccgta gctctaaagt tcatggtaga
    27121 acttgagaag aagatagtag tatttgaacc gcaccgtttc ttcaagatct ttgcgaaggt
    27181 tggcgaggat cgattctctc tttctcgaat cgaagggaaa aagttcacga acaagtctgt
    27241 aaccaccgaa gagaagagag atgaaataat aaccgagctt tccaatgcaa ttcttcacgt
    27301 caaaaacgta aaggaacgca cctccaagag aaacgcgcct atgccattca ttacttcaac
    27361 acttcagcaa agtgcaatag gtgagttggg ctggagtgcg agtaaaacga tgaagattgc
    27421 tcagcagcta tacgaaggta ttgaaaccga tcaaggtcag atagctttca tcacatacat
    27481 gagaaccgat tcgacgagaa tatccaacca ggcaagagac aaggccgtcg aaataatcga
    27541 aagtaaattt ggaaaggagt acgttggtcc catcagaccg ggcaaaaagg gtaagaagat
    27601 tcaagatgct cacgaagcta taagaccaac ttatcctgag aacgatccag agaaggcgag
    27661 acaactttta tcaggagaca atctaaggct atatgctctt atctggaata gattcatagc
    27721 ttctcaaatg gctagtgcgg aatactctgt gaccgaagtt gaattagagg acgaaaacaa
    27781 caagtacatt cttagcttga ccggggagag aagactcttt gacggttatg agaaaattgt
    27841 ctcgagatca tctaagagtg agatagatcg ggactataag ataggcgaag agataaagcc
    27901 ttccatgatc gaatgggagg aagatacaac taaaccaccc tcgaggttca gcgaagcctc
    27961 tcttgtaaaa gagcttgaaa agaaaggaat cggccgcccc tcaacttatg caactataat
    28021 caccactctt ctcgaaagga agtatgtact tcgacaatcg agagaactta gaccaacact
    28081 tcttggtgcg atcgttaacg agtttctgaa tgaatatttt cccgatgttg ttgatatgaa
    28141 tttcacggca aatatggaag aagaacttga cgatgttgaa aacggtgcaa agaagtggcg
    28201 agacgtagtg atgggctttt atagcgggtt caaaactgat cttgatgaga tagataaaag
    28261 aatcaaaaag ggagagttga gaattgagtt cccgacagat aaagagtgtt catgcggtgg
    28321 gtacttcaaa gtcgtctttg gaaggtatgg tgggtacttg aaatgtgaat cttgcggcaa
    28381 gaatgaatca gtcgatatga catccttcac ttgtgtaatc gatgggaagg taatgctgaa
    28441 agatattgct gcgaatcaaa aaatagagat agagatagac gaaaagtgtc ccgagtgtgg
    28501 ttctccactc gtcataagga agggaagata cggagagttc attgcctgtt cggcctatcc
    28561 gaaatgcaag tacacgagga atgttaggat cgacgctccc tgccctaagt gcggaggggt
    28621 ggttgaaaaa ctccgaagca agaaaggaaa gaattacttc aaatgctcaa gttgtggaga
    28681 actcttctgg aatgaaccga caaacgaaaa gtgcgaaatc tgcggctctt atctctttct
    28741 caaggttaag cgaggaggaa agaaggttct gtactgtgag aaatgcaaga aggaatttga
    28801 aattggagga gattaatgtc taaaaggatt gccgttgttt atggagggtt ttccagcgag
    28861 agagacgtgt ctgttaagag tggaagtaac atttcgaaat ctcttgagag gctcggcgcc
    28921 gaagtgatcg gttttgatct caaaagggat agaatcgcga gtctactcag tctaaaggcc
    28981 gatctctttt ttctagccct ccacggcaaa tttggcgaag atgggacagt gcaggggttg
    29041 atggaacttg cgggattacc ctacacttct tctgattcca ggacaagcgc ggtttgcttc
    29101 gacaaagaga ttacatacag gctagtgaaa gcggaagtcg atctgcctct atggaagaaa
    29161 gtgattagcg agaaggatct cgagggctgg aagatatttc catgtgtaat aaagcccact
    29221 agagaaggat cgagcattgg agtgtttatt tgtgacgacg cttcaactct catcaataga
    29281 accaggttac tgctagacgt gtacgactct cttctgcttg aagagtatat cgatggaaga
    29341 gagataaccg tttcgatcat tgattcaaga gaaggcatta aagtcctacc ggtgcttgaa
    29401 ataaaaccaa aaaaacgttt ttatgattac gaagccaaat atactcctgg cttaactgaa
    29461 tttgtcgttc ctgccccgat agaaagaagt acacttgaat cgataatcga aaagtcggtc
    29521 gcggtttaca agcttcttgg ctgcagggat tttgcgaggg tggatgggat actaaaggga
    29581 ggagtgtttt actttcttga ggttaatacc attccgggta tgactgacct gagcgaccta
    29641 ccgatgtcgg cgagagcggc cgggatgacc tttgatgatg tcgttctaga tataattgag
    29701 gcggccgata aaagaaacag gagggaatca tgaagacttt tcatggtact acaatactcg
    29761 ttctcagaaa gaagggaaag acggttatag tcggagatgg tcagataact attggagaca
    29821 ccgtaatgaa aggtacggcg agaaaggtta ggaaacttgg cgatggttca gttcttgcag
    29881 gctttgcggg ctctgtagct gatgcaatga ctctttttga gaagtttgaa gaaaaactgc
    29941 gtgaatccaa ctctaatttg aagagagcgg ccgtgaattt ggccaaggat tggaggacaa
    30001 acaagatcct taagaatctt caggcacttc tcctggtagc ggacagagag agtatcctgc
    30061 tgatatctgg gaacggtgaa gttatagaac ccgatgaaga ggttctggct atcggttctg
    30121 gaggttccta tgcattggcc gctgcgaggg ctcttttgag aaacagccaa ctggaagccg
    30181 aggaaattgc ggttaagtct atggagatag ctagtgaaat ttgcatatac acaaacacga
    30241 atttcaccat ggaaacactc ggaggagaga aacaatgaat agagaagaac ttgacgagtt
    30301 gactccaaaa agaatagtgg aggagcttaa caaatatata gtaggacagg ataaagctaa
    30361 gagggcagtc gccgttgcga tgagaaacag gatcagaaga caaaaactcc ctgaagagat
    30421 aagaagagac gtaataccaa agaacatttt gatgatgggg cctactggag ttgggaagac
    30481 ggagatcgct agaagattgg ccgagctcac gggttcaccc ttcacaaagg ttgaggccac
    30541 caggtttacc gaagtggggt atgtcgggaa gaatgtcgaa tcaataatcc gagaactggt
    30601 agatgtaggc gtcaacatgg tcaagcagga aaagatgggc gaagtagagg ggaaggcatc
    30661 ctatcaggtt gaagagagaa ttctggaggc tctcgtcccc ggacctcaga cgaaagcaac
    30721 gggtcccagg aatatcattg agttgtttca aggccagcaa caaccgaagc cgagtcaaga
    30781 agagaccgaa agaataagga atcgcaggga agactacaga gatagattga gaagcggcga
    30841 tcttgaggat ctcgatatcg aggtcgaagt tgaagatcac agtcagcaga tgataatgat
    30901 tccgggaatg gaagatatgg gaatcgatat gtcaggcgtt ctgggaggaa tggtaccgaa
    30961 gaagaccaaa agaagaaaaa tgagaattgc tgatgctcgt aagacgcttc tacccattga
    31021 ggctgaaaag ctcttggata tggacaaagt tgtagccgaa gcgatcgata gggtgcagaa
    31081 ccgcggtatg gtctttattg atgagattga taagattact ttcagaagtg gttcccatgg
    31141 ccccgatgtt tcaagagagg gagttcaaag agatcttcta ccgatcatcg aagggaccac
    31201 cgtaacgaca aagtacggtc agataaggac cgattacatt ctcttcgttg ccgcgggggc
    31261 ctttcacact gccaagcctt cagatttgat tccggagttc cagggaaggt ttccgatcag
    31321 ggtcgagctt gatgcgcttt ctcaaaagga gtttctgaga atccttgtcg agccgaagaa
    31381 tgcgataaca aagcaataca tggctttgct ggagactgaa ggagtaaaag ttacgttcac
    31441 tgaagacggt ttaaaagaaa tcgcgcgtgt ttcacacagc ctgaatgaga aaatcgagaa
    31501 cattggagct agaagactct acacagttgt tgagaaagta ctcgaagaag tttcattcaa
    31561 tgctcctgaa gttcctcaag aaattgtagt tgacgtcaac tatgttaatg agaggatcgg
    31621 cgaaattgcc gccgatgaag acctgagtgc atttatcttg taatggaaag agtagagtac
    31681 gccgcaatag cactttctgg agatttttct ccatcagaga tggaagagat cgaaaattcg
    31741 ggaatttcag acattcatct tttcagatgt cggaccgatc taaacaaagt taaactgaag
    31801 aggtttcaaa agagttttcc cgatgtggcg aagattctct ccaggtttac gaatttcata
    31861 acattcaagg atgttcaata tcccgaagct ctcaggaata cctttgagcc gccttcagta
    31921 atattctttg aaggtgacgc tggacttcta aattcaagaa acattctcgc catagtgggt
    31981 tcgaggaaag cggatggtta tggagcatcg attgccaaag aatatgcgaa gtcgctctct
    32041 gagaaaggga taaccatagt aagcggtctt gcggtcggta ttgatgctca ggctcacatt
    32101 ggggctcttg aaggtagagg ctctacaata ggagtactgg ggacgggtat cgatgtagcc
    32161 tatccagcga gcaacagaac gctcataaag tcggttaagg aaaaggggtg tgtgatttct
    32221 gaatttcttc ccggaacgcc gcccctaaag cagaatttcc cgaagaggaa cagaataatt
    32281 tcagggttgg ccagggcagt ctttgtagtg caggctacta tgagaagcgg ctcgctaatt
    32341 actgcaagac tcgcgcttga aaacggaaga gatgtttttg ctgtgcctgg agacatcaac
    32401 agaagaaact ccgaaggcac gaattggtta attaagaatg gggcaaaact gatttccgag
    32461 tgcatagaca ttatcgatga gtttcctgaa atttcattcg aggaacctca gtctgggaac
    32521 ttgcagtcaa gagtccttga tcttctgggc aacggtcccc tcacttttgg agaactacta
    32581 ttgatgacgg acttttccag caaagatctg atcgttgaac tcacaaatct gcagttggga
    32641 ggctacatct tcgaagaaaa cgggcgatgg aatagaactt agcgtatttg agctggtata
    32701 atcttgatga aatgtagaag taggaattag attggaggga gaaaaatgcc ttacgtaaac
    32761 acgaaagaaa tactcgaaag agcaaataaa gagtactatg ccgtaccggc acttaatatc
    32821 aacaatcttg aatttctgca ggcaattatc gatgccggag tggaagaacg ctctccggta
    32881 attatcgaaa cttccgaagg tgcgattaag tatgcaggga atggggacat taaactggga
    32941 gcaaagcttt tcgtatcgat ggtcgaaagc tatgcgagga atgtcgatat tcccgtttct
    33001 ttgcatgttg accacggaaa aaacttcaag ataatcatgg cggcaattca ggctggctat
    33061 tcatcagtaa tgattgatgc gtccgaattc ccgtttgaaa aaaacgttgc agagacgaag
    33121 aaaatcgtgg agatagccca tagtcttggc gtatcagtcg aagccgagtt aggtagactc
    33181 gttggaatag aggataacgt tgctgtcgaa tctcatgaag cggcactggt tgacccggat
    33241 gaggcaaagc tgtttgcaga agagacggga gtagattttc tggcgcctgc aattggaact
    33301 agccatgggg ccttcaaatt caagggagaa gcgagactgg attttgcaag actcaagaag
    33361 gttaaggatc tgacaggcct accgctcgtt ttgcatggag cttcaagtgt tccggaggac
    33421 attaaaaagc ttgccgaaag ctatggagcc gatttcaagg gagctaaagg agttcctggg
    33481 gaaatccttg ctgaatcggt gaagtttgga ataaacaaag tgaataccga tactgatcta
    33541 agaatggctt ttattgcttc gttgagagag ttcctttcga agaatcccgg ggagttcgat
    33601 ccgaggagat acttcaagac acccaaggaa ttggttaaga atgttatcag agaaaggctg
    33661 agacttctag gttgttctaa taaagcatag acggaggtgt cgaatgaaga tccttgtaat
    33721 taattgcggc tcgtcttcaa ttaagtacca gcttcttgat gccgcaagtg aaaacgtgtt
    33781 ggcaaaaggg ttgcttgaaa ggataggtat ttccggatca aaactctctc ataggaaagg
    33841 cgagaaaaaa tacgagttcg aaaaggacat tgctgatcac actgaaggcc ttgaactaat
    33901 aattgaggtt cttcagtcag aagagactgg agttgtgaaa aacatcaacg aaattgaggc
    33961 cgtcggtcac agagtggttc acggtggaga gagattcgca tcttccgttt taatagacga
    34021 tgaagttgta agtgagatag aagcgaacaa tttccttgct cctcttcaca atccggccaa
    34081 cattgagggt attgttgcag cgaagaagat tcttccatct gttcctcaca tcggggtttt
    34141 cgatacggca ttccaccaat cgatgccaga atcatcttat ctctacgcca taccttacga
    34201 cctatacaga aaatataaga taagaaaata tggttttcac ggcactagcc atagatatgt
    34261 tgcggagaga gcagctgtca tgatggagag acctctcaaa gaactgaaaa ttataactgc
    34321 tcatattggg aatggtgcat ctgttgcggc agtcaagaac gggaggtctg ttgatacctc
    34381 tatgggattc acaccgcttg aggggctcgt catggggacg cgatccggcg acattgatcc
    34441 ggctattgtt cctttcttgc aggagcaaga gggattaacg gctggggaag taaacgacct
    34501 gctgaacaaa gaaagcggta tgtatgggct tactgaagga cggttcagtg atatgaggga
    34561 tatagaggaa cgcgcaatga aaaacgagcc gacttgcaaa cgggcacatg atatatatga
    34621 atacagactg gcgaaatata ttggcgctta tgcggcggca atgaacggag tcgatgcgat
    34681 agtgttcacc gcaggcgtag gagagaatag tccttacttg agagccagtg tgatcgacaa
    34741 gtacctcgga tatcttggca tatcgattga tgtagagaaa aataaaatca gaggaaaaga
    34801 gaccatgatt tccacaactg attcgaaggt aaaagtcttc gttatcccaa ctaatgagga
    34861 acttgttatc tctagagata cgagtaggat agttaagaat ggtttgaagg aattgaaact
    34921 gtgggaagag tgaaaggtga tgacgagaca ttaaggaaat cgttctggag ggtgatacca
    34981 aacttgccgt gcggggtgag aaagcagaat actactatgt ggtatcgtcg ggcgggctca
    35041 tcctcaaagg attcgattct gaagacaagg tctatctgga aggagatctt gctgacccac
    35101 tatcggtagt tggtggggag cacagcggag atcttctttg tgttggtgat tctgcaattc
    35161 ttgttggtga cagggacgag ttgaaagagt atctggtctc cagaccggaa aggctggaga
    35221 agctgtttgt tgatgttgaa aaagacctga gtttgtgcag tggaaaaggg ccttttgatc
    35281 tcgatgagtt tactgcatct cttgtcgcca aaaagcaatg ttggttagaa gagtatcctc
    35341 ctttgctttt cggagagaaa tcgctctacc gaaagggtat cagactgata gagcgaaaag
    35401 actatccctc tgctcaagag gttctgagaa gctaccttga tcagtatcag aattcccctt
    35461 tgtcaagacc tgttaagctg ttctatgctt ttagttgctt tcttaacgat tttttggaag
    35521 atgccctagc ttcaataatg gatattttgg aatccgcaga agatgaaatc tcaaggatcg
    35581 cgagattttt cgtttgccac atgggtctat ttgaatccgg cttcaagttt ctttatagag
    35641 gtccgagata tagctcggat cttttccgca ttctaaaagc ggattatcgg aggataagaa
    35701 aggctggctc agacagaatc gtctttgaag aaggtagaaa ggctggaagc gtgctctttc
    35761 ttctcaaggg tgagatagcc cttctgaaaa agaggggaga caagaattcg gtactgttta
    35821 cgatcaaatc gccaagctcc attggcgaga tccaggttct ttcaagaagc aaatgggata
    35881 ctacattgaa aatcaagagt aattcggaat acatcctaat cgatagagac aaacttgttc
    35941 agtatttgat acacaaatct ccccaagacg gatttaggat ggttgagtac cttcttggat
    36001 acattagaca aacgagtgct acttgaggct tctcaatgga ttttaaaaat catctcgctc
    36061 tgcttaagga gaatctccgg ttcgaattcc tgtgcttcgg tgaatagagg gtcgttatcg
    36121 ggtactgttg ggaatagcgg cgtgaggttc ctgcttttcg caatactgca gattgcgcct
    36181 attgcctctt tgaaggaagc ggctcttgtt gggccacagt ccttaacata aaaaactgaa
    36241 tccctctcct gccatacgca ataagcaatt tctcgaccct gttcatctat tactgtatcg
    36301 aagttgtatc tctcaaggtg atgaaatgat gcgggctctc tttcccattc tggatttcgc
    36361 ttgtatttgt ccattacatt cagtgcgcat tcaactacct ttcctgaatc agattcttta
    36421 gtcgagaaat cgccaattcc cggccttctt atgaagaaag tgataagttc acgtctatgt
    36481 ctgaatccgt acttctcata gaaagcaatc gctcttttgt cttcttcggg aacttccagg
    36541 acaattcgat cacagccttt ccatttagca attctaattg cctcgtcaac cattttgaac
    36601 cctacgcctt ctcttctaaa accgggaatg acacccataa gatctatcct ggctcttttt
    36661 tctctgaaac tgagcaagac aattcctgta tggatgccct ctacgttcat gacaaatgat
    36721 tccgaaagag aaatgttatt ctccctaacg tctctttcaa agctctcaag agtccagtgc
    36781 atcctgagca cgtagtcgga aaagactaag ttcagaatat tgacgacatc aacccttgaa
    36841 agctcctgga ttctttggaa agagttgtta ttcaactttc ttctgcctcg ctttcctgaa
    36901 gtaattatgt tcttcagtca gttcaatcag gagttcattc attctccaac tgagatttga
    36961 ttcagttgtg gtggcaaaga cccactctct tccagtataa tgacggattg tatcgatcgc
    37021 gtttcccaaa gactcatcgc taaagccatc aaatactgca tagcataagc catcttctcc
    37081 gttgcctgcc ttctctttcc gaaccccttt cccattttcc agtatctctc cgactatctc
    37141 gttttcttgc cccttgcagt cgattaacga gacgtcggcg aataagtcta gtagtctatc
    37201 aaaagcagag ctatcacagt aaaggagaat cgaacgtttg tgtcctgctt cactcattca
    37261 atcacctcga cacaatttta gcatagcgtt atcaacgcta agccaataat cggcaggcaa
    37321 gtctttttcc ccaaaagacg aattagcatt gagatcgaca ctgacaacga aatcataggg
    37381 atgggaattg gggaggcaac ggttgctaca acaggttata attaaggggg aggtcagata
    37441 agataatgca cattggaaga tttagagacg tcagcatgag agtattctgg ggagttgtgg
    37501 aaagggatct tgtgacccct ctcgatatca aagcggaatc cacgggagac tttttccaaa
    37561 ctttagaagg gattccgaat gttatacaaa actttggctt gactgagcgg ataaatctct
    37621 ctgacttaga ggttctctct ccgattacta gcccttgcag aatcatatgt caaggaaaga
    37681 attatctcga ccatctcctt gatacaggga taagaaaaga ggataagaag tacaatatgt
    37741 tcttcaacaa atcatcggct tctgttaccg gtccaaactc accaattgtt aggccaaagg
    37801 gagtccgcct cctcgactat gaagttgagc tcggtttggt tttgaaggcg tctatagatc
    37861 ggcctatcaa tgttgagttt tctcatatgc atgagtacat cggaggaatt gtaataggca
    37921 acgatatttc ggcgagagac attcagttac ctcaatctca gttcttcaag ggcaagagct
    37981 atcgatcatt ctgcccgctt ggacccttta tctttctaac tggcagaaat gaaatgaagt
    38041 atctggagag tttaacggtt gagcttaaag taaatggagt gacaaaacag agagcttcaa
    38101 cttcggacat gatatacaaa ccatgggaaa cactgtcgga actgagcgaa ttttccgatc
    38161 tcgaggccgg agacgttata cttacgggta caccaaaggg aacggctctt agtcaaaaaa
    38221 aaggcttgcg tacttggctc agaagattac ttggcgaatc aaaaagatgg gagattttca
    38281 tcagagaaca ggagaaaagt ggaagatacc tggcaaatgg agacgtaata gaatccagga
    38341 tattctcttc agatggcagg atagacctcg gtgttcaaag aaacagagtt gtttatgagg
    38401 aggaaaatga tcatgatagg aattatctgt gactcagtag tagatcttcc tgtggaattg
    38461 gctgaaagag atgatgttta cgtcgtccct attatggtag tcctcggtga aaaatcctac
    38521 cgtgaaggtg tcgagataag caaatcggaa atcgccgatt atctggactc caactttgcc
    38581 aggacttcac tgccaagccc ggcagacgta acggaagcct tcgaagcaat gtattcaaaa
    38641 ggttatgatg agctgcttgt ggtgaacctc tccagcggtc tgagtggtac ttacaatctt
    38701 ttcaagacga ttggtgaaca gtttgtcagc gaacatcact cggttaaagt tgagtcaatt
    38761 gattccctct ccctttcagg tggcattgcg atgctggttc acaaggcgat aagaatgaag
    38821 gaaagagggg attcactcag ggtgattgcc tcagacctta gaagcagagc tggaataaaa
    38881 aacatcgtct atttcgtttt accgacactg aagtatctga aacaaagcgg tagaattggt
    38941 aaacttcaag gatcggttgg ggaaattcta gatgtaaagc cgatagggac aattaacagt
    39001 ggtgggattt ttactctttc aggtagggca cgtggcatga aaaaagcagt ggaaaagatg
    39061 gtggatcgct taattgttgc tgttaaagga aaaaaagttc tatgcctagc tctttaccat
    39121 tctggaaacg atagcgatac tctcgcgctc gttgaatggg ctaagagaaa aatcatttct
    39181 ctatctgaaa cacttcttac cggagagctg tcttcggcaa tactggtcca tggtggtaga
    39241 gggatcatag gcgttggtgc gctgattgct tgaaaagcgt tccaacacac cggtcgtttc
    39301 gagaggcaag ttaggacaag ataggcaaat aataaccaga actcgaacag ttgatttatg
    39361 acaagtaata ccgagtaaac agaaaataaa agcatcaatc aggagcaata gcgtcttcat
    39421 cagtaataat cttcaataat cacttctcaa agaatcagac tcagtgcaca tagatttcgg
    39481 tgtcattaac gattttgtta taatggaagc gattccaaat cgttgataac tggaaggtgt
    39541 attctcttga aaaagcccac aataaaagat gtagcatctc tttcgggcgt tggaaccggt
    39601 accgtgtcaa gagtgcttaa tggcggttcc gttaaagaaa cgacaagaat taaagtaatg
    39661 aaagcgatcg ataagcttgg gtacaagcca aatcagatgg cgagaacact tgcagggggc
    39721 aagaccaaaa ggattctctt tctcatgccc gagatcagga cggaatttca ctggagagtc
    39781 atgaagtcgt ttgatgagta tctggacaag atgaattatg agatggtgat ttaccctgtt
    39841 ttttccgata aaaggctgaa gaaacttaga agcgatccgt ctcttattca gaatatagat
    39901 ggtgctgtta tctgtacgat gaatattgag tttttatcta gaaagtacat agatttcgga
    39961 accaatgttg ttctatttga atcacaatcg gacgagtttg actgcgtcta tctagacaat
    40021 gttttgggtg gaagaatggc tgctgagtta cttactgaat gtggttcctc agatttgttc
    40081 gtcatctctt tcgatgagga aaacaccatt ttggcaactg aaaattttga aaagagaata
    40141 ctggggttta cgagttatct aaaagaaaaa ggttatgagt tcaatgagga gaaatttgtc
    40201 tacacatcct tcttttttga gacggcgcat gagagaatcg cctcgattct atctcagtac
    40261 gataggccgg ggatctttgc attagcagac aattttgccc ttgaagtttt gcagatggca
    40321 tctgcaatga agaaaattcc gggaaaggac ttctttcttg taggttatga caatcagagc
    40381 tggactgaaa gggctgggtt gactacaatc gagcagccaa tggaagatat gggtagaaaa
    40441 actgctgagc tgattattca gaagattgag aatccgggtc agtatgttca accagtaaag
    40501 tttttgccga gtttgaaacg aaggaagacg gcttgacctg aatctatttt cgaggaggag
    40561 ttatggccag gaaagataag ctatcattag cgcagaagga acagagaacc gcttacaaat
    40621 tgctcattcc cgctttgctg atcttagcat tggtggcgtt ctacccgttg tttcaggtct
    40681 tctatacgag cttcacagat aaggtttttg cgtctggaac agatgaagca ccaaaattaa
    40741 tcggatttga aaactacaag agcctactgg gcttgactgt tatggagctg ccgaaagtcg
    40801 ttgacgaagc aacgggcgaa gcttcaattg atgaggagac tgggaaacca gtttacgaaa
    40861 gggcaattag agtcttgcct agaagtccag taaggtacag agaatggttt cagttctctc
    40921 tttttgggcg tcgttatgta gtgggcgcga gagatccgga gtttatgaag gcaatctcga
    40981 acactcttgt ctttgctgta atctcggtct ttctcgagac agttcttggt ctaggggttg
    41041 ctcttgttgt caacagcaac ttcaaaggta aaggtgtaat gagagcaaca atgcttgttc
    41101 catgggcggt aatcacagtt gtatctgcaa ggatttggga atggatgctc cagccgacca
    41161 gagcaggtct cttcaacgct gtactgagta agttgggctg gggagacgga gacctttcat
    41221 ttctaagtat gccggctctg cagatgccta ctctcattgc tgttgacgta tggaagacga
    41281 caccattcat ggcgctcttg attcttgcag gtctccagct cattcctaga gagctttacg
    41341 aggcagcaag agtagatggg gctggaaggt tgaagcagtt cttatctgtc actttgcctt
    41401 tattgaaacc cacgctggct gtagcactta tttttagaac tctcgatgct ttaagagtct
    41461 ttgacgtttt tcaggttctt atgggaaacc ggatgtactc tatggcctcc tacaactatt
    41521 atcagcttat tggaaacaga aacatgggtc ttgcctcggc tatcggtgtc atcatcttct
    41581 ttctcatagg aatctttgcg gttatctaca tgaggcttat gggagttgat aaagaatgag
    41641 gaaaaacaaa ctgatctggc tcaccgggaa gatcgctttc tggatactgg tgatattcat
    41701 attcttctat atgcttttcc cattctactg ggcggttaac tcgtcgctaa aatccgaagc
    41761 tcagcttcaa atgactccgg cgacctttat tccagtcgat agtgatggga gattcgctcc
    41821 gacgcttcag aactatgttg ccgttttcaa cgacggtacg tttgtaagag cgttgtggaa
    41881 tagtacaata gtagctggtt tgacaacgct tcttgctttg ggagtcggtt cctttgcagc
    41941 ctatgcgatg ggtaaacttc gtttcaaagg caagaagctc actctttatc taattctttc
    42001 cctcacgatg tttcctcaaa taactgtcct ttcaggtttg tatgccgtta ttaataccct
    42061 caatctggga gcaagaataa gtctgattct ttcttatatg atattcacct tgccattcac
    42121 tacctgggtc ctgactgcat ttttcaagga actccccact gagataatgc agtctgcatg
    42181 ggtagacggt gcaacgcctt tccagacttt ctataagatt ttgttgcctc ttacagcccc
    42241 tgctcttgtc acagccggtc ttcttgcctt cattgctgcc tggaacgagt atatattcgc
    42301 tctgactttc acgactatcg ctccggaggc aagaaccatt ccggtagcga tttccttatt
    42361 cactggatcc gtttcgaggc aagtcccgtt tggggagatc atggctggcg cagtgattgt
    42421 aacgattcct gtagtggttc tagtcttcat tttccagcga aggatagttc aaggattgac
    42481 tgcgggagca gttaaaggct gacgattgtt tgaactttaa agaacgtggt ctgaaaaatt
    42541 gggaggtggt ctaaaaggtc ttttaacaga taggttctac aatgagttat tttctacgag
    42601 atcattacaa aggagggttt tttatgaaga aagttctatt gacacttatg gtagcgatgt
    42661 ttgcgttggt agctgtttcc gagacagtta taacagtcgc cgcgggcgcc gttggtcaag
    42721 agcttgaatt gaccaagaag gcagcggaga tgtacatgga gattcatccg gatgtaacgg
    42781 ttaaggtgct tgacacacct gatatggtcc aggatagact gggactgtac ttgcagtttc
    42841 ttgaggcgaa aagcccggaa atcgatgtat accaggtgga tgtaatctgg ccgggcgatc
    42901 ttgcacagca tttcgtggac ctatatgagt acaatgcaga caaagtagta tctatgcact
    42961 ttccggcgat tgtcgaaaac aacaccgttg acggaaaatt ggtagctatt ccgtggttta
    43021 cagatgcagg gcttctctat tacaggacag atcttcttga gaagtatggg cttaatccac
    43081 cggccacatg ggacgaactt gaagaagcgg ccaggataat tcaggagggt gaaagaaaga
    43141 cgaacccgga cttctgggga tttgtttggc agggcaatgc gtacgaaggt ttgacatgtg
    43201 atgcacttga gtggttggct tcaaatgatg caggaacaat cattagttca gacaaaaaaa
    43261 tcacaattgc caactcgaat gcagtcgaga tcctcgagaa ggtagcaggc tgggttggaa
    43321 ctatctctcc gagtggggtt cttacatttg ctgaggagga tgccagagca gtgtggcaag
    43381 caggaaacgc tgcatttatg agaaattggc catacgctta cagccttagc aaaggtgaag
    43441 acagttccgt tgcgggaaag tttgacgttt cgcctcttcc ggcaggcaag agcggtaatg
    43501 gagcggctac actcggtgga tggcaacttt cggttagcaa gtatagcaaa aatccggaag
    43561 tcgcagcaga ttttgcactt ttcatggccg gctacgaagt gcagaagatg agagctgtag
    43621 aaggttcatt caatccaact atccaggctc tttacgctga ttacgaagtg ctaaaggcaa
    43681 atccattctt cggatctctg tatgacgttt ttgtaaatgc tgtagcaaga ccatcaactg
    43741 caaccgctcc aaggtacaat gaagtatcta ccctgttctt caaggcagtt cacagtattc
    43801 tctctggtac tgcggacgcc aggaatgctc ttgaagagct cgctctcgat ctcgaagatc
    43861 tgacaggatt tgaggttgtt tggggattct aaaaccaagt tgcgattatt cgctggctcc
    43921 atatggagcc agcatatctt cagttctaag cggaagaagg tggagagatg aggagtgcac
    43981 aggtaaataa gaagctactg aaggattatc agcccttcgt tgaggaagaa gtcattgaaa
    44041 gtatcatcga gctttcaaga gagctcaagg gtctcagggt tctgcatgta aacgccactt
    44101 ccttcggtgg cggagttgcg gaaatccttc atacactggt tccgttaatg aaggattgcg
    44161 gactggaggt tgactggaaa gtgatcgatg ctcccccaca gttcttcgat tttacgaaaa
    44221 gaatgcataa cgctcttcaa ggaaaaaaag atttcttgtc agaagaagat aaaagactct
    44281 ttgaaaaggt ggagaaagac aatgcggctt acatcggggt cgatgaatac gatgttgcgg
    44341 tgttgcatga tcctcaaaca gttgggcttc ctgcctttgc aaactttgga aattgcaagc
    44401 ttgtttggag atgccacata gatacttctt caccgaacga ggctttttgg aattacttga
    44461 atacattctt gtcttactac gatgcgggga ttttcacttt aaagagttat gccaaagatg
    44521 gaattgccat cgacaaaatt tatgacattc caccatcaat tgatcctctg agcaacaaga
    44581 acagaatgct atctgaagag gaaatgaaga aagctatgac gaagctcggc cttgtttctg
    44641 gtagaccggt tatagctcaa gtatcgagat tcgatccatg gaaggaccca atgggagttg
    44701 tcgatgccta tagacagctg aagaggaaat tcccggatct tcagcttctc ttaatagggt
    44761 ctatggcttc agatgaccct gagggatgga agatttacga ggatctcctg agatacattg
    44821 gaatggacta tgacgtaaag gttctttcaa actttcatgg cattggcgat attgaagtaa
    44881 acgctgctca aaggattagt aaggtagttc ttcagaaatc acttcgagag ggttttgctt
    44941 tgactatgag cgaagcaatg tggaaaaaga caccagtgat tggtggaaat gtaggcggaa
    45001 taccccttca ggtccatcat ggtgtaaatg gatatcttgt tgagagtgtg gacgaaaccg
    45061 ttgaatacac agaaagaatt cttgagaatc ctgatctagc taaagagctg ggtagaaatg
    45121 gatatgaaat agttaagaaa cattttctct gtaccaggca tctactacag tatctcgagc
    45181 ttttccgcga tttgacgaga tagtggaatt cccgtccttg tgttaaggga cgggggtttc
    45241 cgatttctat aagagttttc tagctcttat tttctggagg ttttcataca ttggaagacc
    45301 gtttaggtaa agattaggtg cctcaccctg tatcagtgga agagcataag acctgaactc
    45361 gtcagtaaca tagaatccgt cttcggagat gaattcaacc ggaagatctt ttgtaccatt
    45421 tgcaatgagc tcaagagcga ccgtagtgta ttctttaaga tagggtttgc ttctcaatct
    45481 atttatggaa atcatgaccc cgctttctcc tcttatggcc tctctaacgg caagtgctcc
    45541 tacttcaact gcttcttgcc aatcgatcaa gcttgcaacg tgcctcgatg atctctgaag
    45601 gtaatcaggt aaggccacgt gaactttgca cttcaaagcg tctctaatta tcttctcaag
    45661 gtactgacct atctttccta attgaacatt tccaaatgcg tcgtaatagc cggcagttga
    45721 tatgaatgaa ccatcaggcc ttttgagagc ctctgaggca actattgagc agtatccttc
    45781 cttcttcaca acttcttcga cttttgctat aaaagagtcc tccataaagg gaacctcagg
    45841 aaggaggatg atttgaggac cgtgaaacct gtctgtgctt gcaagtgaac cagatgcggc
    45901 aagccatccc gaattacgtc ccatggattc cataataaag acctttgttg aatcactgta
    45961 cattgatttc aggtctctgg tcgcctccat tatcgatatt gcggtgaatc ttgcggccga
    46021 tccataaccg gggcagtggt cagtttcaag aagatcgttg tccactgttt ttggaattcc
    46081 aacgacttgc agttcataac cgatctcttt agcctttttg cttattttgt gagcagtttc
    46141 cattgagtca ttgccaccgt tatagaagaa atatcggatc gagtgcgcat cgaagacttt
    46201 aaatagcttc tcgaaatctt catcactgtc tatcttagtt ctacaagaac cgaaaactgc
    46261 acctggagtg taaggaattc tgtctatctc acttcttgct tcttcagtta agtcgtaaag
    46321 atcctcattt agaatcccct ttactccatt tattccagcg aagactctat cgatttcttt
    46381 gctttcaaga gccgcaacca gcgttccata agcagatgcg ttgattactg aagttacacc
    46441 acctgactgc gcgtagacca cattcttcat tcttccaccc ccaagaaaat ataccacact
    46501 ttgtgagggt cataattaaa aaaacagacg cctgggggcg tctgtgagaa tcaacgatga
    46561 actatcggca agcttcggta atggttggca ctgtttcgga agcgttaccc caaatagtaa
    46621 tgacattctc cattgaatac agagtattct ccacaccgga gtaaccgggt ttttcatcga
    46681 ggttgcatac aattacgctc ttagatttct caacttcgag aatcggcatt ccatatatgg
    46741 gagtaccttc ggccgtatta gcagaaggat taacaacgtc acatgcgcct attacgatcg
    46801 ttagatcagt gtttttgaac tccgggttta tcatatccat atcgtacaat ttgtcataat
    46861 cgatgcctac ttcggcaagc aagacgttca tatgtccggg cattcttcct gccactggat
    46921 gtattccgat tttaacttcc tttccagccc tctccagaca gtcaatcaga tctttgactt
    46981 taccctgtgc ctgcgctaca gccattccat acccgggcac tattattact ttctttgctt
    47041 ccgagattat ggttggtaag tctttggcaa cttgttcatc tcttttgctt gagggggaag
    47101 gagagttatc gatttgctca attatgacag gcactgtttc ggaagcgtta ccccaaatag
    47161 taatgacatt ctccattgaa tacagagtat tctccacacc ggagtaaccg ggtttttcat
    47221 cgaggttgca tacaattacg ctcttagatt tctcaacttc gagaatcggc attccatata
    47281 tgggagtacc ttcggccgta ttagcagaag gattaacaac gtcacatgcg cctattacga
    47341 tcgttagatc agtgtttttg aactccgggt ttatcatatc catatcgtac aatttgtcat
    47401 aatcgatgcc tacttcggca agcaagacgt tcatatgtcc gggcattctt cctgccactg
    47461 gatgtattcc gattttaact tcctttccag ccctctccag acagtcaatc agatctttga
    47521 ctttaccctg tgcctgcgct acagccattc catacccggg cactattatt actttcttgg
    47581 ccgagtttac ggtttctccc actttcatat ttcgtgttcc cttttcattg atctctttaa
    47641 ggatacttga ggaatgcggc gtattgttcg gctttcttaa gtttgacact gtagtcattc
    47701 cagtcagtac agcgctcaag ttcctgttca ttgccttgca cattattctt gttaggatta
    47761 gacctgagac tccaacaacg gctccaaccc ctacaaggag agggtcctca actgcgaaac
    47821 cggttatcga ggcagccaca cctgaaaagg agttcagcag agagattact acgggcatat
    47881 ctgcccctcc gactcggaga gccattatca ctccatagac tgccgatatt atcgttaaga
    47941 gaactaccca aacgacgtat attcctgtga tgagtgtgct taggaagact attaccgcaa
    48001 ttacgaagag cataatttgc tgcagctttc cgcccttatc aatatttaca ggtcttcctg
    48061 gaatgattgc ctgcaacttc attgctgcaa tcatacttcc tgtgaatgtc aacataccaa
    48121 cggacaatgc caaagaagct gtcagccata aataccagct cgtacctgca tggtacagac
    48181 tgctccccgc aatcgctact agcgccgaag cggcaccacc gaatccgttc agcattccaa
    48241 ccatttgtgg catctgaatc atcttgacta catgaccgag tgatacacca gcaaaagcac
    48301 caattacaag ccaaagccaa agggcagagt ttccaatccc tccgtaagag actattgtgt
    48361 aaattatagc agctcccata aaggccgccc caattctatt accgagaaga gcggtctttg
    48421 gagactgcat taatcggatt tgaaggatga atccaactat gaatattgct gctatgataa
    48481 taacgctcat ttgtctgttc tcctagcact agaatcgaac atctttagca tacgatcagt
    48541 cacccaaaat ccgccgacta cattgatcat tgccagtaca agagctgctc cgcttagaaa
    48601 aagcactatt cctgaggtag cggttgcgaa tacagcaata gcacccagaa cagtaatacc
    48661 agacaacgca ttcatacccg acataagagg agtatgaaga agtgatggta ctgaagatat
    48721 tatcctgtag cctataatgg cacaggcgac caggaaaact atcaaagcca agagcataaa
    48781 atcctcctct tatttcatta gataattact ctgatatttc tcccatagct aggagagtac
    48841 cgtgatgaat tatcgaccct cctagagtga caagagtctc tttcacaatc tggtcatcgg
    48901 cgtcagcaca gatctttccg tctttcacca agttgtccac gaagtgccac atgttactgg
    48961 aaaacattct tgtcgagtct atcgagagtg tagcagggat gttaagcatc cctattatcc
    49021 acactgaatt gtaatcgtat tccttgcctt cttctgtaag ttcacagttt ccaccctgat
    49081 cgatagcaat atcaactatg acagagcctt tcttcataga gtcgaccata gttttggtta
    49141 ccaaagtagg ggcaacctct ccggggataa gagccgaaag aataaggatg tcacatgttg
    49201 caagatgctt agagagaatc tctctttcct tttcgtacca ttcttttggc agcctcttag
    49261 cataaccacc ttttcccata gcaagttgct gaggcacatc aaacggaata acctctacgc
    49321 caagcgattt tgcctgtgac gcagcttcgg gtctaatatc gagtgctttt acctttgcgc
    49381 ccaacctttt agctgttgct attgcctgaa gaccggctac tcctacgccg acaaccacta
    49441 cttcagacgg aggtatcatt cccgccgaag ttggcatcat tggaatgaat ttcttaatcc
    49501 tgttcgcacc aaatagaact gatttgtaac ccgccacggt actcatcgag gtcaacgcat
    49561 ccatggattg agcttttgaa attcttggaa tactgtccaa ggtaaaactt gtgatatctc
    49621 tttcggcaag cgttcttatg gtcttgtggt tgacatcatg tgccgggtgt aagaaggcaa
    49681 cgattgttgc gccacggttg atcatttcag cctcatgttt cccaatcgaa ctgttgtact
    49741 gcggctcttt taccttcaga atcaagtttg ctcttgagta aacttcatac ggagtcgaga
    49801 ctacttcagc tccaacagct gaataatcgc catcgaagaa gaaagccccc tctccggctc
    49861 gactctcaat gatgacctta gcaccgtttt caacaaattt ctttgcaaca tctggagtca
    49921 cagcaacgcg gagctctccc gcaagaatct cctttggaac accgatcgtt agtcccgaaa
    49981 acttcattca gaattcctcc ttaacaatgt ttgatgaaag cacagcctca ttatacaata
    50041 gagcgtgaag aagcgaaaca gccgaatttc gcctctggaa attgataaga accagatatt
    50101 cgcatgtact tccttgccct tcgtgattgc aaaacgatgt attacgatgg agtgctttac
    50161 agtcaatatt gacgatatcc aattaattat cctcgctttg aagtaaccta tctaactgtt
    50221 attgtaaagg tagctgtagc ctccccgcct tttagatcat tagcggttag cataactgtg
    50281 ttcgatccca gctcttctgg aatgaaggag taaatgcttc ccgtaagaac tccgggtcca
    50341 tagaccagtt cataccttag atagtctccg tccgggtcga tgctaaactg tctgaggtca
    50401 agccgcaagg ttctagtcgc aatggttgtt tgatcaggca cattaaactc gggtgggcga
    50461 ttggtgtcgg taatcagtag atcgaaggtg gaactggtct ggcctcccct tccatcagaa
    50521 gcggttacta caattgtata aaaaccgtaa tcttcgaaac ctggttcaat tatcagcctg
    50581 cttccttcga tcgtccccat tccctgaacg atctcaaatg atagactgtc attgttcgga
    50641 tcagatgagt attcactgag atcaacaaca agtctggaac cttctgctat cgtaaatcca
    50701 ggtatattta attctggtgg cctgttgctc tccctaacat caattctgag agtcgccctt
    50761 gcagacccgc ctttcccgtc tcctacagag acaactgctt cataagttcc tgcggtgcca
    50821 tagtctgcgt cgaaggtgta ggtttctccc tggatagttc cagggccgga gaccagtgag
    50881 aagctaagta aatcgttatc aggatctgaa gcgtattctg aaaggtcaat cgttagtaca
    50941 tcaccctccg cgattcttct aggtagaatt gaaattgttg gcggattgtt cgtccgaact
    51001 acagtgagat ttgccgttct ttcaatgaat tctccctttc catcggaagc tcgaatagtt
    51061 accgagtatc ttcctggttc gttcgactgg aatgtgtacg tcgagccttc gattcttccc
    51121 gggccattga gaagctggaa tgaaagcgaa tccccgtcag gatcattaaa ataattctcg
    51181 aggttcaatc tgtattgttc actctgaaaa acgacgcctt ccggaatact tgaagctagc
    51241 ggagctctgt tcgcggactc aatgtttacc tgaaagcttt cttcgacgac gttctttcca
    51301 tccgatattt ccaagactac tgtcttggaa ccggcatccc cgggagggct cacataggag
    51361 taaagagttc tttgaacctc acccggtccc gattttaagt taattgaaag tggttcatcc
    51421 tctggatcgt tcacaaaacc tctcaggtcc agaaccaaag tgtttccttg ctcgagtgtc
    51481 tgatccggga catcagcagt aggtgggaga ttctgtgcat cggccacttc tgtttccgaa
    51541 gatgtgactg ggctgtctag acccgtagcg gcggtttccg atggtacaga agatgtggag
    51601 tctgcggtca gctctgttgc ggatgtagca aaggctttat tagatatcat gaacgttgaa
    51661 actgccttct ctcctcttcc atctgaaacg gaaatagtca ccctctcata atttgcactt
    51721 gtgaaatcag gttcataagt gtagttgctt ccaattatct gcccattgcc ctcggttaat
    51781 gaataattca aggcgtcaag gtcaggatca tttgcatact cgtcaaggtt tatggaaatc
    51841 gtctcccctt cggcaaccac ctggtctggg atagaaagct cgggtggacg gttgacctct
    51901 ctcacggtga tttcaaaaga agactccgcc atttgttcct tcggatctac tactgaaatt
    51961 gttacagagt gacttcccga ctgttggaaa ctcggtgaaa aaatgtagaa gttctctttg
    52021 acctctccgg gaccagaaat ttttgtataa ctcagagacg tcatcttctc gtcgcttgaa
    52081 tagtcgtgaa gatctatcga aagcgtgctg ccctcatcga taatcatatc ggggatgctt
    52141 attattgggg gtttgttaag catgtcaggc aggagtcgta ttgcaaaaac aacaaccaca
    52201 acgaccatca acaaagccca gaagaaccat tttctcatac cgcataacct ccatgaaaaa
    52261 cataataatt atcagttcca ttatagttta ttcctgttcc atgccaaaat gaattcattt
    52321 gcgggaaaat aacctcgagt atcctaatag tcggctaaat cagttcttta ttgtcgctaa
    52381 acacattaag attatacttc gtttctacgc gggcgaattt taacctaata tctggcataa
    52441 ggggcaatca atatgctaga atctatttga tagaaaaatc acaatctagt ttcttcagct
    52501 cagttaatgt agattttgaa tggaggtgct gtctggtgcc cgtaacaatt accgtgaacg
    52561 gcaaggattt tactgttgat gaaaaccaga gtatactgga agcctgccgt gaagtaggaa
    52621 tagctatccc gactctttgt tatcaccctg cgctttcggt cgtaggttca tgcagagttt
    52681 gtgttgtaga ggtagagggg gctcgcaacc tctcacctgc atgtgcaact caagttgctg
    52741 atggaatgaa aatcaagacg aattccgaaa gggttaatag agcaataagg tttaacctag
    52801 gtcttcttct ttctaatcac cctaatgact gtatgacctg tgatgtaaat ggcagatgcg
    52861 aatttcagga tctaatatat cagtacgatg ttgaagatat gttcccaaag acactgagaa
    52921 atgttccata tgatttctcc agtccatcgc tttttagaga catgaacaag tgtattgact
    52981 gtgggagatg tgtgagagcc tgcgatgagt tgcagggtct ttcgattctc tctccagtga
    53041 acagagggta tcatgttctt cctctgcctg ctttaggtgc gcctattgcg gccacggatt
    53101 gtataaactg tggtcagtgc tcggccgtct gtcctgttgg ggcaataact gagagaatgg
    53161 aattcaggga agtggtcgcc gaacttagaa aacatgataa ggttttgata gctcagaccg
    53221 caccatcagt tagggtagcc ctttctgaag agtttggaga gcttcctgga acggtcagca
    53281 caggtaagat ggttgccgcg cttagaaagc ttggattcga ctatgtgttt gatacgaatt
    53341 tctctgccga cctcacaatt atggaagagg ggtcagaact tcttgaaagg gtaaaagaag
    53401 gcggaaagtt cccgatgttt acttcatgtt gcccgggttg ggtaaacatg atggagaagt
    53461 tatacccgga gttcaccgag aacctctcaa cggcgaaatc tccccaggag atgatgagcg
    53521 ccctggttaa aacgttcttt gctgagaaga taggaaagag gccggaagac atttatcttg
    53581 tgtcgataat gccatgtaca gcgaagaagg acgaaaaggg cagagatact ttgacagtta
    53641 ggggggccag atctaccgat gcggtgcttg taacaagaga acttgggaag ctgattaaga
    53701 tgcacaagat tcccttcgag tcgcttgaag aggaagaata tgacagtcca cttggaatct
    53761 ctaccggcgc tgcggtaatc tttggtgcga caggcggtgt tatggaagcc gctttgagaa
    53821 ctgcatacga gcttcacaca ggggaagaac tcccaagact tgatttcgat tttgcccgtg
    53881 gatttgacgg ggtgaaggaa gctgaaatcg atatgaaggg caccaaggta agagtagccg
    53941 tagttcacgg gggaagcaac gttgtcaagt tgatggacaa aattcgttca ggagaagctt
    54001 tctaccattt tgtcgaagtc atggcttgcc cgggcggatg tatcggaggc ggtggccagc
    54061 ccaaatcgag ccagcccggt tatcttcaga agaggatgga agctatctat aatattgaca
    54121 aagcttcggt aatcagaaaa tctcacgaaa acccggcgat tactaagctt tatgaagagt
    54181 atctcggaaa gcctttagga catctttctc acgaactact gcatacccat tacaaagaca
    54241 acagaaagag tgtgatcgag agaaagaaag aacttgagga aatagccaaa gggtaggttc
    54301 tcaaagctgt atgagttttg aaggggcctt atggctcctt tcgttttggc tcagtcctcc
    54361 ttctttagca aagtgagaaa tacggcaaat ggtgtcttga agaggcactt gatatcgaga
    54421 ataaatgatc tattcttcac gtaatagagg tcaaaggcgg ttttaatccg gtattcttcc
    54481 agcgttgtag tgtatttgta gtttatctga gcccagcccg ttagacccgg ttttacgaga
    54541 agtctagacg aatagtcctc gatgttgtcc attccaagtt tgtgaaatga tgggagctcg
    54601 ggtcttggtc ctactagact catatctcct ttgataatgt ttatgatttg cggaagttca
    54661 tccagtctga gaggtcttaa gaatttgcca agctttgtta tcgtttcctt gtttcccttc
    54721 gtgtcgaaag tcctgaactt gtacattccg aattgcttac agtccttgcc gatccttgtt
    54781 tggcagatga agatgggtct accctccaag atcagagaaa ttagagagac aataactgta
    54841 accggactga acagaactaa cagtattact ccaataccaa catccaagag tcttttgaag
    54901 ctatcatatg ccacttttgg ttcaatcggg tcattagagg tcagcataaa gctttcttcc
    54961 ttgaattgcg atttcacacg tttgtagatt atgaaatttc tgacgaccgt tgctcccaac
    55021 gcaggaaggg caacaaacgg tgatacagga aggaagactg gaaaaacaaa gcaagacata
    55081 acagcagatc caagggtaga agccatttgt ttttctttat cagagatcgt gattccctcg
    55141 taaccgcgca ttgaaaaact gatcagaaca ctcgcacaac aagctgcaat gaatgcacca
    55201 agaccgttca ttaagagcgg caaagccagt aataggtcaa tcaaatagaa aaggctgttc
    55261 atcattatat tttctcatct ttgtgtagat atttgaactg gcaatttctc acaatgttga
    55321 gtaatgacta tgtttttggg gtatcatgta acttgggagg aagggattga aaaaactata
    55381 ccttagtgat cttcatattg gcgacgggac gttaaaggat gacttcaaat tcgattccga
    55441 tcttgtagag ctgatattga gctcaaagga aaaagcattc agagaaataa tcgttgttgg
    55501 cgacggactc gagcttttga cttcagagtt tgtaaagaaa agaggactct tgccgttcga
    55561 tcaactcatc gatcaactgg atgtgtcgat aatagatgtt attgagaaca gacacaggga
    55621 ggtattcaga gcatttcggg agttcaccaa gagtggaaga ctgatatatg ttgttggaaa
    55681 tcacgactca tttcttcttt tcaatgaagg tcttagaaag aggctcactg aattcctggg
    55741 tgacagtgaa agtgtacaga taaaaccata ctatctcgac agagagttca agactcttgc
    55801 tttgcacgga aaccagtatg acatagtgaa caggtttttc aaggacagga gaagcggcga
    55861 gttaatgcaa ccattcggtg actacatggc acgcttcatg atgaaaaatt ttgataaatg
    55921 cctcttgaga agagagcttc ctgaacaggc agttagggat tatcagaaca ttcatcctac
    55981 tctcgatgtc tttcagtggt tcgattttat tagaagagta tatgatgtag attcagatct
    56041 tcaggatgaa tgggccagac agttccttct gatgctaaaa agtgatgcgg cacagcgttg
    56101 gatagacgcc aattggccaa tgttgaaggt tattgaaaaa ctattcatca acaaacacgg
    56161 tggaatgaaa ctcggtagtc ttatgattag aattgttatg gcatttagaa agctgaagaa
    56221 gactgataac ctttacaaac agggcaggag attgcttgga gccgaaggta agaagggctt
    56281 tcgtaaagct atgaacaacg aattctttct gatgtacgga gataaagctc ccgcaattgt
    56341 tcccggtgag ctgaagggcg tgataatggg tcataatcac cgtcatgtcc ttagaatgat
    56401 ttcgagcaac ggagaagaga aattttatgc caatactggg acatggaaac cggttgttga
    56461 aatgattgat gacgatccaa ggaacggttt tgccagaaga gtggagctgg gatatattac
    56521 tatagagatt gtttctaacg agtttctagt tgagtcaaag catataaaga agcttagtgg
    56581 ccttcggttt tctgaagata cactggataa gaaaaccgag tggaattctg tgcgcgagat
    56641 tggaatgcga gtctgaggag ggatgtaaat tgcactatta tagaggttct tcgatagtgc
    56701 cgataataat caccttgctc ttagtaattg ttgcggccgt agtagcggcc tttttcctct
    56761 ggcccgttcc agaaccgaaa atacttatct cgaatgtctc accaattccg aaaaatgcag
    56821 agagaatcca gtatgctgaa aaggtagaat taaggtggga agctgtttat gaacgtccca
    56881 ggcatggcct cttagcagaa gtcttcgctg gcaaggaccg aaacagtttg accagattgg
    56941 cacttgacct tcagggtgat ctgacctcgc ctaccgaggg aatattttcc ttttcccacg
    57001 tgtttgaggc tgatcctcac tctcagtact ggtggaaagt aagggtttac acagaaggtg
    57061 gaaaatccgc agaaagtgaa atctggacat tcgtactcat gaatacttat cctagaaggc
    57121 cagagctagt tccaccaagc agtaacctgg gaaacgtccc tctaaaagat ctgacactta
    57181 actggagttc caatgatcct gatggagatg agttagtata cgacgtttat tttggcagag
    57241 agccaagatt agatgagaag gacattctca ttagaggaac gaacgaaaca atggttgatg
    57301 tttcgttgct taagagactt gattactcga cgaagtacta ctggaaagtc gttgcaaagg
    57361 actcgtatgg aggcgaatct acttcttata ctgagagctt cacaactcag atgagggcag
    57421 atcttcctgc agttacgccg gaaaatccaa gaagcggagc tagcgatttt gatgcgggca
    57481 gaggatttct ttcgtggaag acttctttgc ctctcgagta ctatcttgac gagctagatt
    57541 ttgatgtcta cctttctgag ggacagtcaa gatattcact aattggaaca acaaattcaa
    57601 ccgagattgc tgttccttct ctcaagggcc atacaactta tagttggtat atcgttgtta
    57661 gagatgcatc tggaaaagaa aagaagagcg aagagtggat cttccggact tctaacagag
    57721 cacccataat cgagatggag tatccgaata tttctccagg gcaggattct gtgccggtaa
    57781 aatgggaagt aatagacccc gatggagata gtgttacatg cgaagtattc tttggaccgg
    57841 atggtgatga gcctgccaga atcgcttcag gtagaatgac atcgattttt ctctcagcac
    57901 ttgatgaaca aacgaattac acactctcta taagagctag cgatggtcaa ggaggagaaa
    57961 ccataagaga tatttctatt accccgggta atagacctcc ttcgatctat ctttctcggc
    58021 cggtgtcgat ggagtttgtc gaaccctcgg ttgtatcatt caactggttt ggagaagacc
    58081 ccgatggtga ttctcttaac tacatgctcc atttgaaatc gaagaatgac tcaagaatcc
    58141 taggtccaat cgaatctcaa gaatatattg tgagagacct ggaagaaaac gagaattaca
    58201 gctggtttat tacggtagaa gactctagag gtgccttcgc tagaagtgaa gacgctgttt
    58261 tcactacagg gcagaagtct ctggaagtag ctgttccgtt gacgcctcag cctggtgaag
    58321 ttgaagtaag cttatctgac actactttta gctgggaaat cgatgatgag agcgaagatc
    58381 tctcttatag attcgttctt ggaaatgacg acaagttgaa tgacataatc tttgaaaacg
    58441 aaacgaagga aacttcgatt gaattgacct tggacctact tagcaacaag aagtactttt
    58501 ggagagtcga tgttatcaag aacggttctg aaactgaggg agaagtctgg agcttcacca
    58561 gcgaaaatct acctccggcg ttaccagagc tcagattccc tgtggatgga gcttcgggag
    58621 tattgaccaa tgatttgact ctaagttggg tttccagtga tcccgatgga gaaattgtgt
    58681 cgacaaaggt acatctgcga gattctgctg gaaatgaaga ggagttcgaa gctctcggca
    58741 attcactgac catcgaagag cttgatcccg gtcaacgtta cgaatgggcc gtaaaggtta
    58801 ccgatgacgg gggaaaaagt tctgtcagcg aatacgcatc ctttgttact ggaaaccaga
    58861 ggccggttat aactttaact catcctccta gaatgttcct ggagggggca acgactcctc
    58921 ttgttgttga gtgggagcta gacgatcctg aaggagaaga ccttgaggtg ggagtatatg
    58981 ttaatcttgc cgaagagtcg ttagggcggc cagtagctat gggaaataac cttgagagat
    59041 atgtcataac caacctcatt tcaggcagag attacttgtt gacaatcatg gcagaggacc
    59101 ccggaggtga aatagggact ctcgaaatgc ctgtaaaaag ccgtgtttcg ggactagact
    59161 acatctatcc ttccggtgga gtgttcgaga acggcagaga gttctcttgg tcttattcac
    59221 aacagaattc gggatacgtc ttccggcttt atgattcaga tcataacccc attgtcagaa
    59281 gagttgttca ggacaacaaa tatagacccg aactaccttt tgaggacggt aggcagtact
    59341 tctgggctgt atctgttttg agggacaatg tagagtatgc aggtgaaccg gtcagtttca
    59401 tttctggaca gtcgtttgga gtgttgcttg aagcgcctag gaacggagaa aaggtgccgg
    59461 cttcgggagt aacattgaaa tgggttttag acggtgatac agaagcgata aggcagaagg
    59521 aagtctattt tggagaactg ggtagccttt cacgttacac tgtttcgggc gacagttatc
    59581 atacaggaag gcttcttgcg ggacgaacat atgaatggtt cgttcagttg acagacaata
    59641 gtggagccac aataagaagc gagactttcc gattctcggt tgaaaacagg cctccagaac
    59701 tttctttaat cagtcccgtt aatggaaggg cggttggtga aacgtatgtt gatttaacat
    59761 ggtcaggcta tgatcctgac ggagatcagc tgagatactc ggtgtatttt ggaactgacg
    59821 aggatctaga gctttacaga gattccatgc agtcaaatca gattagaata tccggccttt
    59881 tgatgaacaa gacatatagc tggttcatta cagcttcgga cggtcaaaat gttgttaaat
    59941 ccgaaacgag aacattcaat gtaactgcac caaaattcaa gattgacatc ctgtccccgg
    60001 tagtggattc ggaagtagat cttaccccca ttttctcctg gcaatcttct gaagtacgaa
    60061 gtggggagtt cagagtgttt attggtgagt cagagtcgga aatgtcactg atcggtataa
    60121 ctgatgccac ttctatgcga ttgcagaatc gattggataa cggcaagacc tactattggt
    60181 cagttgagct ctggcaggaa ggaaagatga tatcctcaac cggtccaaat agattcaaga
    60241 caatgtcgtc cgagagacct atcgcttctg agagtgtcga tacgttagct ttctacttca
    60301 acgaggtttt gaatatagtt tcactgagca gtgctggcct tgaaatcgtt gagatccctt
    60361 atcagtatga tggaaagaac tccgcaattc taaacggtga tctactctat gtgatagacc
    60421 aaagtggaaa actgtccaca atcaactacc gaaccggtga ggccaaagtt atcgacactc
    60481 tagttactaa cacggttcca gagaagttga ttatggaggg cggatatctc tggattctag
    60541 acaccaagag tgcagggatg gtcattagag tagttctcaa cgaaagtggc attcccgaaa
    60601 gaatggaaag tgtttacagg gactggacga caccggttga tttattcgtc acgcaagatc
    60661 tctcgagaat ttatctggca gatgctttgt cgggaataaa aatcctcgag agagaagggg
    60721 aaacttacaa cgatcgttct tcaagttttg aagctccctt ggatggttat tccagagcag
    60781 ttgctgaaag taacggtgtt gtttattccg gagaggcagg aatagacggt ggtttgaagg
    60841 tcattgatat aacgagatcg agaaagacta atgtcggaag gtactacatt gttcttgaaa
    60901 tagaggtgtc gggcagtgtt ctttacgcgt ctaccgataa gggagtgtcg gttgtagaca
    60961 ttagtactcc agattctcct gtaattcttc gggatttgga gctcacccag gtcgatgaga
    61021 tttcagttga gggaaggctt ttgatagtga gatcgggaaa cagcatgtta atttatgatg
    61081 tagagaaacc taatgatcca gtcttgctcg agagcagaaa ggaatagaat tccattttgg
    61141 aggtgttagc gtgaagaaat tactcttggc agtattagtt acatttcttt cagtagcagt
    61201 gtttggcgta agcgacagcg tactggttat ggcaattgag accgagccgg taggattcga
    61261 tccgacgctg gttaccgcat ttgcatctca cagagttctt gagaacgttt acgatggtct
    61321 tctgaagtat gatgaaaata tgaatctcgt tcctaatcta gccgaagatt tcgaagtggt
    61381 ggatccctac acaatagtgt tcaaaatacg agaaggcgta aaattccaca atggcgagac
    61441 tttgactgtt gaggatgttc tctttacttt tgaaagaata agggatcccg aagtaaaggc
    61501 tccagcagcc agttattacg gcgaggtaga aacaataaga tctttcgacg gaaacaaggt
    61561 tgagttcaag ctgaaaatcc cgatggcttc ttcgcttctt cccaattttg cgggagttaa
    61621 cagcgctata ctttccaaga gttttgtcga gtcaggcgca aatcttcagc tagagacaaa
    61681 tggtacgggg cctttctaca ttgctgagtt tgtcgcaggc aactacataa ggctgagaaa
    61741 gaatgcggac tattttgttg ccgggcttcc ttatctcgat gaaataaaaa tgatgataat
    61801 gcccgaagag gtaacaagag tttccgcttt aaggaatggg gacgtcgata ttgcaaagat
    61861 cagcgagcca ctaagtctta atcaactggc ctcagacaag ttcgtaattt acaggacgcc
    61921 agtgctaagc tattatttgc tcgggttcaa cacgacaaga ggacctctaa gcaaaccgga
    61981 agttagaaac gccctcagtt acgccgttag cagggaaatg atagtgaagg ctgttgcctt
    62041 tgacgaggca acagttacgg gtccgttgaa cccagagctg gatttctggg ctttacaacc
    62101 caatcagttc gaggagtata gttacaatcc ttcaaaggcg aagcagctac ttgcagaagc
    62161 cggttatcca aatggcttcg aatttgagat agttgctgcg cagagataca atttcgataa
    62221 ggttgcacag gtaatccagg cacagctggc agaaatcggt gtcactgcaa agataaactt
    62281 ggttgagtgg ggcattttca tcagcaagtg gagagagagt gatttcgact ctttcatttc
    62341 tatgaatagc ggatctatag atcccgacat ccagttcaac aggactttcc gaactggagg
    62401 atcgactaat gtctttctct atagtaaccc tgaggttgat gatctacttg ataggggtag
    62461 aagcgagtcc gatatgaacg ccagaaaatt gatctatgaa gaagtccaga gaaaactcgt
    62521 ggaagaatca ccaatactct ttctctattc gccaaacacg ttatttgctt ctaatgattc
    62581 cgttaaggga tttagatcat tggcgaatga aagccttgtg tttctgagag aaacaagcaa
    62641 agattagttt cagagtagaa tatggagttc ggttacgtaa tcaggaggct gctatctgca
    62701 gttcccacag ttcttttagt cacgttcatg gtgttcattg cgattcacat agttcctggc
    62761 gatgtgatag atataatgct gggaacccag aattacctga ctgaaagtca gatagaggat
    62821 ctatattccg aatatgggct tgacaagccc ctgattgttc agtatgggat atgggttaag
    62881 aatctgcttt cattcaacct gggtacttca ctaagaacgg gcagacccgt aaccgaactg
    62941 ataaaggaaa gattcccggt tactcttgaa ctagccgctt tctcattagg atttgcactt
    63001 atatttggaa ttcccatggg aataatctct gcagtaaaga gaaatggttt tctcgataat
    63061 tttgtgagag tgatcggtct aattggtctt tcgtccccat ccttctgggt gggggcgatc
    63121 ttaattgtgc tggtttctgg tgcctttgat aatttcaacc tatttggata tgtgagtccg
    63181 gtagtcgatc cgttttctaa tctacaggta atgtttcttc cttcacttac aatgggcttg
    63241 atggtagcag cgcagatttt gagaattacc agaacatcga tgctggatgt actaaatcaa
    63301 gattatgtta gaactgccag agcaaaagga gtgagtgccc gaaatgtaat tctcaagcat
    63361 tctttgagaa atgctctaat tcctgttgta acgctctcgg gtattcagct aggctatctg
    63421 cttggtggaa ccatagtaat agagaatatg tttgcccttc ccggaatggg aagacttctt
    63481 cttcaggttg tcaatgaaag agattatccc gttgttcagg gaattgttct ttttatcggg
    63541 ataataatag taatgttgaa tatcttggtt gacgttatat atacactaat tgatcctcgt
    63601 gtcgagctac gttagggtga gcgactatgc ttagagtaat gcggagactt gtgagtaatc
    63661 cgatctacac gattagcatg atcatgatct ttgtcattgt tctgatcaca ctattccctg
    63721 gtttattcgc accatatgac ccttacaaga tgaatatgga cgcctttatg agcaggcctt
    63781 ctatagatca tttgtttggt ggagaccagt ttggaagaga cgtgttctca aggtcgatgt
    63841 atggaataca gaagagcgta ataatcgctt ccagtgcgat tgctatttcg gcgataatcg
    63901 gtactatgct ggggctgttg tcgggctata ttggcggact gaccgacctg ataatcatga
    63961 ggattatgga tagtttcttc gctttcccat cgcttattct ggcactattt ataattgctc
    64021 ttttcggttc aagcatgtcc aacctaatct ttgcaatcgg ccttgtctat gtgccgatat
    64081 ttgcgaggac cgtaagggga gcaacgcttt ctctaaagga tactcttttt gtgaaggctt
    64141 ctaaggcgct tggaaaaaag aatatctcta taatgcttaa agacattctt ccaaacatct
    64201 cttcgatcct gattgtaacc tttaccacaa atgtttcgac agcgcttcta accgaggctt
    64261 cccttggttt tctcggtcta ggagttcctc ctccagagcc tactctagga ggaatggttg
    64321 gccagggaac ctcctatttg ttgagcgcac catggatagt cctgtttcct ggaatgatga
    64381 tagctataat cgttttgagt ctcaacatcc tgggggatgg gctgagagat gtgctcgatc
    64441 ctagaataaa cagataatca ccatctatag gtccacaagg ccgtggaact agtagaaact
    64501 ccatcaacat ggttcttcaa catttcgatt tcctcaacgc tgtctataag acctgcagca
    64561 actatggtag tttcaggtgc atgaactctg attttagggg ctaccttcgc tgcgactatt
    64621 ccaggcagta tctcaatgtt tctcactcca ttggatacga tcgtttgaat tcccttttca
    64681 acggccctcg agtcaagtac gaaaaacctc aaaaggctct gatgcatccc agcatttact
    64741 gcttgtttga atattctaag cttagcagtg attattccat cgactccgct cttcttaaga
    64801 taaagaacgc tatcctcgtc gccagaaagc ccagaaacaa aatcaatatc cacgaagacc
    64861 ttctttccag cagctttgaa tcgatcgatc ctctcctgta tttcaaagag cccaccattt
    64921 aggaagaaaa ccgtttccgg actaactgat tcaggtctct cttttctgtt ccataaagcc
    64981 gcaataattc cctttagcat aaaacccctc ctaagtataa taatacacta agcgctgatt
    65041 tcgaaagaag gacccctcct gttctctgaa gggtcacttt tattccggct gtgtgtatgg
    65101 aatatgatat aattctatac acgtggtaga gtttatgaga gaaccacgcc atttttgtat
    65161 ggcgtggttt tctttttgga ggtgctggat gcaagcggta gtcattggag gtggagttgt
    65221 cgggtctcta atcgctcgag aactcagcag atttagaatt aaggttgttc tcgttgagaa
    65281 atcagaggat ataggacagg gagttactaa agcaaactct gctattcttc acggtggcta
    65341 tgacgatccg ccaggaacat taagagctag gttctgcgct cccggaaatc taatgtttga
    65401 taagctctct gaagaactga aattccctgt aaaaaggacc ggatcgattg tcgtggcaaa
    65461 ggacagaaac gagcttccaa aacttgagga actgcttcaa aatggtatca acaatggagt
    65521 taaagatctc agaatcgttg gcaaaaatga gcttagagag ctggaacctc acatttcaga
    65581 tgaatttaat tatgccctat tttgtggtac tgccggaatc actgagccct ggatggtggc
    65641 gattgcttcg gctatgaatg ttgctggcaa cgggggcgag gttatcacag gtgaagaagt
    65701 cataggcggg aagatcaatg aaggaagggt caccgaagta tttctaagtt ctggcaggaa
    65761 gattgaagcc gacttggtaa taaacgcagc aggtctcttc tacgaaaagg ttgcttcagc
    65821 ctttaacgtg aatgtgcccc cggttcattt gagaaaggga gagtatatcc ttttggataa
    65881 aaaggcaagc gaattagtaa atgtgataat atttccgctt ccaaccgctg caggaaaggg
    65941 aaaattagtt gtcccaacgg ttgatggagg agttcttcta gggccgactt cagttgaatt
    66001 acccgagttt tcaccggaag atgttagcac tactgattct gggctgactt cagttagaaa
    66061 atctggagaa tatcttatcc cgggaatcga caatccaaag tggttcataa agtcatttgc
    66121 cggactgaga cctgaaaccg tccaaaagga cttctacata aagagagccg aagaacttgc
    66181 aaacttcatc acagtggggg ctatgaggtc tccaggtttg actgcagccc ccgcaattgc
    66241 ggaattcgta gttagtacga tcgttccaga aactggaatc gatctggtta gaagagacga
    66301 ttttgttccg tgccttgaag agagagtaaa gataaaggaa cttccagctg ggaaagtctc
    66361 tgagatgatt gatgaaaacc ctgcgtatgg gcgaatcgtc tgtcaatgca atgaggtttc
    66421 tgaagccgag atagtccagg ccattcgcga tggagcaaga acaattgacg ggattaaatt
    66481 tagaactaga gcgggttttg gaagatgtca gggtgggttc tgcagctgga acattgcgaa
    66541 gattctggct agagagctca agaaggatct atccgaagtt agacagaaca gcgaggaaag
    66601 ctggatagtt gatgggaagg tgagacaatg aacagactca ccacagacgt tcttgttata
    66661 ggtggcggag cagcaggtat gtcatcagct ctcagtgctg caaaaactgg agctgacgtt
    66721 actctcctag aaagagaacc gtttgcagga ggtgtcctaa atcagtgtat tcataatggg
    66781 ttcggccttc agttctacca tgaggaattg acaggtccgg aatttgcctc tcgccttcaa
    66841 cgtgagatga gtgaagaagg ggtgagcatg atcggtgaat cctatgtccg gcacatagac
    66901 gtgagggaga aaaaagcagt tgtgctttct cctaagggag catttgaaat ttctgcaaag
    66961 gcccttgtca tatcgagcgg agccagagaa agaccgttcg gctcgctgtt gatacagggt
    67021 tatagaccgt caggaatatt gccggcaggt gtggctcaaa gatatgtgaa cctggagaat
    67081 tatctaccag gaaaaagagc ggttgtacta ggatcggggg atatcgggtt gataatggct
    67141 agaagactga cacttgaagg tgtggaagta atcgctgtaa tagaacggat gccatatccc
    67201 ggaggtctta caagaaacat tgttcagtgc cttgaagatt atgatattcc ccttctcctt
    67261 tcgacaactg tgaccaaagt cttcggaaag ggaaggcttg aagaagttga aataactaag
    67321 gtcgatgaga gattcgttcc gataccggga acttccagcc ggctgaaagt tgacacttta
    67381 atactttctg ccgggttgct tccacaggtt gaggatttcg atgatgattt gactatcgat
    67441 cctatcaata gaggttttgt tgtttcaaac acaggtgaaa gctccgtaga gggtgttttt
    67501 gcggcgggaa acaatgttgc ggtattcgat ctcgtggatt atgtagccgc cgagggctgg
    67561 atagccggcc gtcatgctgc tctctatgct ctcggcaaga atacttctgg ggacagggtt
    67621 cccgttgtta ggggtgataa tgtgggagtt gttgtgcctg gcatagtgga tatggatgag
    67681 cacgtacgcc tgtatgtaag attgaaaaac cctatggacg agggcactct agttctaaag
    67741 gaactagggc tcgagaagag tttctcttat ggggttccaa gtgagatgat tcagatggtt
    67801 attaacaaag agaagctgat gcctcttatt gacgcaaaac ggttaacggt ggaggtgcac
    67861 taatggagag acacataact tgtgtaatct gcccggttag ttgcaaaatt tcagttagaa
    67921 aaagcggaag cgagtatgag ataaggggta acagatgtcc cagaggaaaa gaatatgctt
    67981 taaatgaatt aatcatgccg aagagaatac ttacaacaag cgtaaaagtc atcaacgggg
    68041 agattcccct agtttccgtg aagacatctg gcgcaattga caggtcgatt ataaaggaga
    68101 aaatggattt gatcagaaaa attgcagttg aagcgcctgt acagattgga gacgtcctta
    68161 tggatgatat cgatggccag gggactgcgc tcattgccac gcgtcctgtg aagagaattc
    68221 aaacttttct tccttaatcc tactacttga aataacgttt gattcgggtt aaaataatca
    68281 ttgtgttcca gcgtggctca atggcagagc atccggctgt taaccggacg gttgggggtt
    68341 cgagtccctc cgctggagcc agaactcccc ctcttggggg agttttttat atccagctct
    68401 gtcctgggac cttgggaaag agtcttgttc ttgaaacgtt gtctagagaa cagacatatc
    68461 ttgtcagtct ttcgacacca attccacaac ctgcagtcgg aacaagctct cccccttctg
    68521 cgatatccat gtaccaagag agttcttcag ggtcgttgcc cttgaaaccc attctttcaa
    68581 gaattctctg atgctcccat tctcgttctc ctccggatag agtttcacca aatccatgag
    68641 gaagtattag atccatatcc ttgagtgttc tcttatcttc cgacaacaag tcatagaatt
    68701 ccctctccca tattgggaaa tcaattaacc aaaacggctc gctaaaactg gcggatagta
    68761 tcgtttcaaa gtcttttcca tactgttcat aagcgtccat gaatttcacc gtagcaaagg
    68821 gttttgaagg aatcgaagga tccgaggaaa gaaaatccat ttcttcgctg caatttctct
    68881 ttactgaaag cattactgta attatgagat cttcgatgag agacattatt tgctctctct
    68941 ttgcgttccg gatttcaaga tcaatttgac tgaactcaaa gagatgtctt cctgaaagtg
    69001 ccttttccga tgtttcaagt cttacattcg gagaaacaat gaagatcctt tcaaatgatt
    69061 tacacgctag ttgcttgtga aatatcattg actgcgtaag gcgatactcc tgatcgtagt
    69121 aagtgaacct tgtggtgtga acttcgtgat taagaggatc ggtgatcgga gatacaatag
    69181 taggaagtag ctcgacaaag ttctttcccc taagaaaact gcttgcttct cccagaattg
    69241 tagattttac ttttactgcc gacttcacaa ctggatcact aaggtgctcc aacactctag
    69301 aactgagtct cccgatgctt tccattttct caccccttca aaaattaagg ccgatggtta
    69361 tccatcggcc ttctaatctt ttttgttgaa acgcaaagat taactgaccg atcccggcca
    69421 gttattatta gaaagtgcta tgctgtatga aactaaaatc aatctttcca tattccttcc
    69481 tcctgaatga ttcattctac catctgcaaa gtaacacaga aagcatttcg tttaagattt
    69541 gttgaagtat ctttgttaaa tagtgctcaa tcggtttata atactagtat gaatattatt
    69601 ccgattttcg ttccacatga gggttgcaga acgcgatgca tattctgtaa tgaatactcc
    69661 gccactggag tcactggagt tcccgatgag aaagagatca tttccactgt agagaagtat
    69721 ctttcattct ttagacatcc agaggatgta gagcttgcat tttatggtgg cacatttacc
    69781 ggatttggta atcagcagaa gtacctggag gttgcaactg agttcttcaa agcaggtcat
    69841 ataaagggga tcagattctc cacctctcct gggctcatat ccgagaggtt aatagagtat
    69901 ttgaaacctt atccggtaaa cttcatcgag cttggagtcc agtcttttga tgatactgtt
    69961 ctggagaaat ctaacaggga ccacaacacc agcgatgttt ataaagccac tcacttgttg
    70021 aatagcaacg gaatcaaatt tggcattcac ttgatgaccg ggctacccga agactcagag
    70081 agtaaggaca ttcactcgac cagagaggct atcagagtag gtgcctcttc cgttaggctt
    70141 catcctctgg tgattttgaa gggttctctt ctagagaatg attacaaact tcgtagattt
    70201 tcgcctcttg acctccagga gtctattgag attctctgga aaatgtacct tctgctctcg
    70261 acatcgaatg tgaagataaa caggattggt atctgtctgt atggaaagca gatagacaac
    70321 gtggtggccg gtccatttca ccctgctatt ggagaactcg tgcgtgatag acttttcctt
    70381 gaaattgtca gggcgttttc cggggaaatg aagagaaacg agctaagact tcataataaa
    70441 ttcaaaccat ttttcacagg tcataagaag ggaattgtcg aggaagcccg taaggagggg
    70501 attcttatag acttcgctca cgatggtgag gaaatcgatg tttctcttta tatgagacgg
    70561 atcaaagaaa gggttctgga gggagtttat gctgaggctt aattcagttt acataaaagg
    70621 attcaagagc ttcgccatgc caactagact cgaaatctca tcgggcatga ctgcggtagt
    70681 cggacccaat ggcagtggaa aatcgaatgt tgttgacgcg ataagatgga tatttggcga
    70741 gcagtccatg aaaaatatca gggccgacag cagggaagat gtcatcttca acggttcaga
    70801 gagatttcca ccttcaaact ccgctgaagt caaacttgta ttcgaatcag aggaaggaat
    70861 cttttcaatt gcgcgggaga tatctagaga tggacagtcg tcttacaaag tcaatgacaa
    70921 acagtcgagg ctgaaggata taaaggagct cttccaggga accggtgttg gaatggatat
    70981 atactctatt gttgggcagg gacaggtcga taaggtagtg acggcctcgc catacgagct
    71041 gagggctctc attgaagagg ctgccggtac tgcggtgtac aaagagagga agaaagaagc
    71101 gctgggaaag ttggcggcaa cggaagaaaa tctaagcaga cttgaagaca taatttttga
    71161 gcttggcaaa caacgtaagt ctctttatct aaaagcaaaa agggctgaga agttcgttga
    71221 gtattctgcg aaacagaagg agctgaagaa tctcttcttc ggcaatatcg caagactcga
    71281 agaggagaaa cttcagacgc ttaacggaga tcttagagag gttcgtgacg agttgaaaga
    71341 tctccagaag aagctaattg aaggtgaatc taaatggtct gctttaaggg cagagttctc
    71401 agaagttgac aaagaaatcg aagggtttac gaaacttctc gaagagtata agaaacggca
    71461 aaacgacttg ttggagctca aagagatgta ctcaagaaga ctgaacgaaa aagagaatag
    71521 attgatagaa atctcaaccc ggattgacaa tttcagatcg gaaattgaag atctcgataa
    71581 gagaaaggat gagattaagt taattatcga gagcctcgag caacaggttt cagatgaaga
    71641 aactgccctc tctttgtctg aagaggaacg ggattccctt ttaaaaaatt acagcgcgag
    71701 agaacgagaa tggctcaagc atcaagagac ctttgagtct atttcgaaga ggctttcaaa
    71761 gattgagaat gagctcgaga gacttgaaaa tagtagggaa gacacaagta agagattgcg
    71821 cctaatcgaa aaccaactgg aaacgaaaag agataggttc gaaaccctca aggaagaaac
    71881 cgagtctcta gcaaaacagg gcaaggaaag ttcagataaa caacgaaaag ttgaagcaga
    71941 agtgagcgcc tcaagagaaa gactcgttga gctggatgca gaattagaga aagccaggga
    72001 tcgtctttca agtaatgaaa gcgagtttag aagatcccaa atggaaagga gcttactcca
    72061 aaggcagcaa gaagaatatc agggattctc caaagcagtc agagaagtct tctcgagaaa
    72121 ggagagcttt gcgggactca gagatgttgt tgcaaacatc atacaggtac cagaatcctt
    72181 tgaaaccgct ataacagttc ttctaggata ccgtatgcag gatattgtgg ttgacgactc
    72241 aataaccgca aagagaataa tcgaattcct gaaatcctat aagataggtc gggtaacctt
    72301 gcttcccatt gacatgattg aaggcagttt cagaaatttc tccagggttg agtcgcatcc
    72361 aggcttcgtc aactacgctg ccaggcttgt agaaatcccg gatggtttcg agaaactacc
    72421 tgtttacctt ttcggaaact caattgtagc aagaactctt gatgatgcga ttgaaatgag
    72481 gagatcagaa ggtttcattg gtagaattgt ttccgtcgat ggccagttgt tgagtagcgg
    72541 tggatccatt accggagggt ttattggaga agagactcga accgatctac tctcaaggaa
    72601 gaggcggatc caagaacttg tagaaagaga agacgaattg gagaagcaaa tacagctcgg
    72661 taataagcag atagaaagac ttagagacga gatgacggag gtccgtggat atctgaaggc
    72721 actgcaggaa gagctcaatg aattggcctc aaagggggcg gcaataaacc gaatgatagc
    72781 ggaattgcta aaatccgctc aggaaattga ggaagagata gctgagcttc agaaacttga
    72841 aaacgaatat actagaagga tggaagaaaa ctctagaaag aaggaagttc ttgttggaga
    72901 acaatctgct ctcaaagaag agcgctctga actacagcgc aaagttgaag aggaatctga
    72961 agagcttaag aaacagaaga aagcccttga acagcttcaa gaaaagattg tcgataccag
    73021 gcttaggcta tccactctat acgagaagca ggaacagtac acaaaggagc acagttcctt
    73081 aattcaaaag agcaaagctg atcaggagaa catcgatctt ctttctcgag aagtctcaga
    73141 agtcgaatcg gagacggaac ggctgagaag acaagtggcg gatcaggaaa gagagctaac
    73201 ctcagtcaag aaagaaacgg aagacctctt ttcgagcata agaaatcaaa gggaagggaa
    73261 agagcaaaga ctttctgccc ttcaagaggc cgaagaagag ataaatgaaa tgaaagcgga
    73321 gagagaaaaa cttcgagaga aatctcacaa acttgagatg cacattcaag agagcgagat
    73381 gaaactgtca agagtgagag aagaattaga tgaagtcgaa aaagaggtcg agattctttc
    73441 cgaagagaaa cttcaagaca tcaaggtgga gcttgacgat tacgagaaca aactaaagtt
    73501 tcttggaagc gttgatcttg aggctattga tgagtacggt gtcgtagata aggagtatca
    73561 agaactcgat gagcagaaga aagatctaga agaagccaaa gtaaaactta tcgaactgat
    73621 tgataagaca gatgccaagg cgaagaatat cttcatggaa accttcaaca gtgtgaacaa
    73681 gaacttcagt cggtacattg aggagatctt cgacggagga gaaggcgaga taaagataat
    73741 ccctggtgaa gacttacttg agaccggttt agagatcaca gtcaggagac ctggaagaaa
    73801 gattcagaag cttcaactct tatccggtgg ggaaaaggca cttgttggaa tagcccttgt
    73861 tttctcgtta ctgagtataa aacccagtcc tttttacgtt ttagatgaag ttgacgctcc
    73921 actggatgat ttcaatgccg aaaggttcag agtgttgctt aagaaacatg ccaccgacac
    73981 gcagtttctt gttgttaccc ataacaaact tgttatggag gttgcgaatg ttcttcatgg
    74041 agtgacaatg acagacggtc tttcaagagt aatccctgtc gaactgcagt cggttgagac
    74101 agttatcggt tagtgttgtc gtgtgaaact agccgcctgt attcccttcc tataaccaat
    74161 aacatccctg ctccgaccag tgcagagcct gcaaaaaaca acatggaagg taaatttccg
    74221 agaaaaacaa tgtcggcgat tactgtgaaa acggggattg tgtagaccca tagagtagcc
    74281 gcctttcctc cccaaagatc tattcccttg ttccacatta tgtatcccag agccgatgca
    74341 aaaatcccta agaacaaaat gcctgagacc gcacctaaag aaaagactac gggttttttg
    74401 aaaagaagga tttctgaaag agcaaagggc gcggaaaaga gaagtcccca gagcattatt
    74461 tctagagttg tccatatcga gctgttgtga atcttctcat agaagtaagt gtatgccgtc
    74521 caggagactg ctgcaccgaa agagaagaga tttcctgtta cgctagaacc atcggcaaat
    74581 cttcccgaat aaataatcaa tgagagtccc gaaaaagcta cgagaccccc caagtattca
    74641 aagagatcgt acttttttct tatcaggtca taaagaagta ttgtgagtat gggagccgtg
    74701 gatacaatta aagaagcatc agcggcattc gtcatggtca aaccataatt ttcgaagaaa
    74761 aaataaaatg tgatccctga gagacctgct aaagccgcat tcttgtgatc taccgtattg
    74821 aaagaacccc tcttctttga ggatatggga aagagaatag caactgcaat tgcaaaacga
    74881 agaaaagcaa gcagcattgg aggcagcgaa ctgacggcca ccttcgtaaa aatatatgat
    74941 ccaccccaaa tcactgccgt tgtcgccaga ataacaagac ctagggatct tctatcagtt
    75001 tttttcataa cctcttatac ggtgaataat gaagatctga tctgaaaatc aaaggctcca
    75061 taataagcgc ctcaaacggt ccgagatcaa cagataccgt tccgaacgac actctaaatg
    75121 ctcttccagt caacagatcc ttcatttctg tcccgttcat aaggaaaccc tcctgcaaaa
    75181 aaactctcga ttctacgttg tttgcagtag ggttgactac gcaaagaacc atatcatcca
    75241 caatttcgcc agttctcttg aaggatatga gtgaactatc ggaagaaatc aagggtttga
    75301 aattgcccgc tcgaagtgat gcctttgttt ctcgaatcgc gttcagcttc ttataaagga
    75361 tccgcgtctc tgtttccttc gaaacacttt gccaatccat cggagccctg ttctcaggat
    75421 cattgcctcc atccatttct atttcctcgc cataatagat caagggaatc ccgggaagac
    75481 aatactgaag aacaagcgct gcttttaccc tctggaagga tcctagctcg ttcttcagcc
    75541 tgggtacgtc gtgtgaagag agaatagtcc aggaattaga aatgaaagtc aagccggcct
    75601 ctctaatagc ctcatcgatg attttcagtg cgtcgtgtcc ggaaagataa ccgttgagag
    75661 cctcccagat taccagtcga aagtagtaat tcatcagccc gtccagatgc gattgctcgg
    75721 gccaaccacc gggatagttc cagatttccc cgataacaac tgcgccactt ctgatcgatt
    75781 tggtggtcgc aactatttct ttcaagattc gtggtccaag gtcataggca acatctagcc
    75841 tccagccatc tatgcccttc ttaagccagt gagctacaac tgagtcagtg cctctaaaaa
    75901 ccatgtttga aaggatcgga ttctcaagat tgagctcaag aagattactt gagtccatcc
    75961 agcctcttaa cccctttttt ccttcgaaga agtactttct gaattcctca tttccctgga
    76021 gagcgcctgg ttcctcgaat agctcaagag agttggccca ttttcccgaa gctccaacgt
    76081 gattgaaaac tccgtccagt attatactga gccccttcga atgagcagtc tcgacaagta
    76141 gaaggaattc ttcttccgta ccaaagtcag gatctatctt aaaataatca acagtgtcat
    76201 acttatgatt cgtgtgtccc cagaatattg gattcaaata aagagtgttt gcgccaaggt
    76261 ctcttatgta atctagctct tcggttattc ctgccagatc acctccccag aaatcatatt
    76321 gatgactacc gtctacggaa gcaacgggtt tttctcccca atctctaacg tcttgctttg
    76381 gaagctgata atgaccttgt tcgcgttttt gaaatacgtt ctttccattg ccaatcttga
    76441 atctatccgg gaatatctga tagacgatag aatctctttg ccagtttttc atcttttctc
    76501 ctccaggaat tcattcgcat aagcaagata gaccattcca aggaaaatgt tgtttctgat
    76561 ggaccagagc tcttggggtt taatatttat tccggattgc ttcagccaag aattcaaagt
    76621 ccaaagcgtc atctcgtaga tttgcatcag cccccttgaa tgcacatagt cagaaaatga
    76681 ttcatcgata cttctttctt ccatcaaagc aaagggattc aaagaggatt caactaccat
    76741 gatcgatagt acaacttccg ggtccagtct agataaatcc agagtcttaa tttcgcctcc
    76801 aactataata tcaactttca catttgcaaa ttccattaag gcattgtaaa tctcctctga
    76861 taggcgattc ttaaagattg cggatctgtc agaaagtaaa ctgctggact caattaacga
    76921 agctatctta gtttctaggt agggacattt tctctttgca ttttgctgtg aaagatctga
    76981 tattattttg ttgcagacga aaagatcgtt ctccgaaaca ttgtgcatct ttactgaact
    77041 gctcaatcta taattcagtg agccgctttt gtcagtgaaa tatctgtatg cttcaaccac
    77101 acggtcatcg tactggtaag atgggctttc aagaataaac ttataatcgg ctccaaggcc
    77161 tgcattataa agcacggaga gagctgcaac tttgtcaagt ctattctttg cgctaaggga
    77221 agtaagtatc aatgaaaaaa tggtgatcga tgcaaggaga atcgaaagca cgatcatgtc
    77281 aattttctta cccttgtcca tattccacct cgcggcaatt atagcagaga aaaagccggg
    77341 ttgccccggc cctcttgttt attgactcta tctctttttc ttctcgggtt tatcgaactc
    77401 acaaacacca accggacatc ccggagagtt tagatgagag agaaactcgt ccttgaacct
    77461 gtcgagtata gatcgcagtg gaattgggac agactggccg agaccacaga aagatgattc
    77521 ttccattgtc tcagatatgt catataactg gttaaacatc tccacagttc ctcttccctt
    77581 tgagaactcg ttaagaatgt gcacgattac acgggttcct tccctgcaag gggaacactt
    77641 tccgcaggat tcgtgagcga agaactccat gagattcttt gcgatatcaa ctgcgcaatg
    77701 agtttcatcg atcgcgagaa ttactccgga tcccagggat gcaccggact tcttgaagga
    77761 atcaaaatcc agctcagcat ccatctcttc tggtttaata aaggtccccg cagcgccgcc
    77821 tgtttgcacc atttttaggg ttctcccctt ggagatgcct cccccaaatt tgtgaacaag
    77881 atctctcacg gaagttccca tgggcacttc aactattcct ctcttaacaa gatccccgca
    77941 caaagaaaat acctttgtcc ccggtgatga ttgtgtccct atcgaccgga accaacctgc
    78001 tcccttatct attattggtg gaacacaggc aaaagtctcg acattattta ctactgttgg
    78061 cttatcgtat agtccactta caggaggata agggggtttc agcctgggtc ttcccgactt
    78121 gccttctata gactctataa gagcagtctc ttcgccacaa acgtacgctc ctgcacccag
    78181 tctgacagaa agatcgaatg aaaactccgt tccgagaact gactgtccca aaagcccgta
    78241 ttcatacgcc ttgtttatcg cgtcccttag acttgataca gattcgaagt attctcccct
    78301 gatatatatg tatcccttac ttgcacctac ggcgtatccg gcaataacca taccttctat
    78361 aactgaatga ggatctccct ccatgattaa ccgatcctta aatgtccccg gttcgccttc
    78421 gtcagcgtta cagatcacga acttctcatc ggatctgact ttctgagtaa actcccattt
    78481 cagtcccgtt ggaaagccgg cccctcctct tcctctgagg ttgctcttct tcagctcttc
    78541 tatgacttcc tcaggagtca ttctcagtgc ctttgcgagt ccctgatatc catctagtgc
    78601 aatgtactct tcaagatttc gcggatcgat agaaccggcg tttctcagga cgattctcac
    78661 ttcactcgtt gatttggtag cttcaaaact tcctccagaa tcgaaatccg caacgaccag
    78721 gtctcttact tttcgaccct ttagaaggtg ttctaggaca attttctcaa tgtctacttc
    78781 ggactttgca gaatacatta catcatcggg gagtacaaga aagagaaccc ctcttccata
    78841 ttcaccgaaa gagccagttt caagaatctc caccatggaa ctcaggttgt aattctcgac
    78901 cagagttttg agataattct ttaagtgcct ggcacctagg agcaagctgt tcgaatctat
    78961 agatacgaga accgtgattg gtttgctcat aatcactcac tcctttccct tgagacgatc
    79021 tcgcgtatta tttcagagac tcttcttggg gtaaggtttc catatgcttc atcatttatc
    79081 atcataacgg gggagactcc gcagagaccg agacaactag tttcttccag cgtgaacaaa
    79141 ccatcttcag tagtttcacc gaagcctatc tcaagcgtgt tcttcaaaga atctatcacc
    79201 gcctgtccac ctgttacatg gcaaggaagg ctcttgcaaa ccctgacgac gtacttgcct
    79261 ctcttgtgag ttgagaacat cgtgtaaaat gtaagaacct cataaatcct agaaagaggt
    79321 gttccagtca attccttcaa cacttctgct gcttcgattg gaataaagtt gccgtaatga
    79381 tcttgaattg cgtgcagagt atttattagt acgtctccct tctcaaggct ctgaccactt
    79441 atatcttcat agattctcgt tacagtcaaa cggacatctt cagcaggcat ataagttcct
    79501 cccttcaacc ctcttcgaag tagcaaagaa acccccgatg aagggggttc caaaaagttc
    79561 acggagatac tttgctgatc gctccaaatc tgcagactgc aagacaacta ccgcatctag
    79621 tgcagatttc cggatctatt tcatggggtt ttcttaccga accgcttatc gcatttacag
    79681 ggcaaactct tgagcaggca gtacaaccca cgcatttttc tttgtcgatc actacacgtg
    79741 tcagagactt acacttcttg gcggggcatc ttccttcctt cacatgtgct tcgtattcgt
    79801 tccagaaata acgcatcgtc gatattaccg gctgtggagc cgtctggcca agtccacaaa
    79861 gagaagttgc catgatgttc tcgccaagat ctttcagaag ttggagatct tccatagtac
    79921 cttctccgct tgtaattctt tcaaggattt caagcatcct tctcgttcca tctcgacaag
    79981 gcgtacactg accacaagat tcttcgaccg tgaactcaag gaagtatttt gcaacatcga
    80041 ccatacagct gtcttcatcc attacaatca ttccaccgga accgattata gttcccagtt
    80101 tcttcatgtt gtcgtatgtt attggggtat cgatcaattc tagaggaatg catcctccac
    80161 ttggtcctcc ggtctggatt gctttcagtt tcttcccacc gggcacgccc ccacctattt
    80221 cgaatatcag ctttctcaac gtgattccca taggtacttc gaccaggccc gtgtttttaa
    80281 cattcccggc aagggcgaag accttggttc ctctcgaccc ttctacccca aactggctga
    80341 accattcgcc accattattg attatgggtg gtatattggc ataagtctct acgttgttca
    80401 gaagggtagg tttttgccag agtcctttat ttgcgggaaa gggaggttta actcttggta
    80461 ttcccctctt cccttcgatg gaattgatca gtgcagtttc ctcgccgcaa acaaatgcgc
    80521 ctgctccgat tctgatctcg agatcaaatg aaaagtcagt accaagaata ttttcgccca
    80581 ggaaaccgta ttctctagcc attttcatag cgtgagtaag cctttcgatt gccagcggat
    80641 attcagctcg tacatacacg aaacccttct tagcaccaac aactcgtgca gcaatagtca
    80701 tggcttcaac tatcgagtgg ggatcaccct caaggaccgc tctatccatg aatgctcccg
    80761 ggtcaccttc gtccgcgttg caaataacgt attttgtgtc accttccgcc tttttcgtga
    80821 agtcccactt catccaggtt ggaaatcctg ctccgcccct gcccttaagt tcgctcttct
    80881 taagtatctc aattacatcg tctctaccaa tttcaaaaag gactttgtgc agcgcgaagt
    80941 aaccatctct agcaatgtac tcatctatgg aaagaggatc gattactccc aggtttctgg
    81001 tcgcaattct cacctgctct gtgaaaaagg gcagttcctc ctgaggtttc tctcttagat
    81061 cccccgcatc tcctctataa agtaggtctt caactactct accctttaga atatgctctt
    81121 ctacaattcg agatgcgttt tctggagtga tcttctgata gaagacacct tcagggtaga
    81181 tcacaagcat agggccgagg tcgcaagcgc ccatgcatcc agtttctaca actctgacaa
    81241 cgttatctag cgaatacttt ctcagggttt cctcaaaagc gcctttgaca ctcttctcgc
    81301 ctgccgaaat gcaggcccct ccagcacaga tcagcaccgt gttctcaact gcgggcaccc
    81361 gtaatcacct cttcgttaaa gttctcctct tttcacaata agatctccga ctacttttcc
    81421 cccgacaatg tgttgttcga ccactctctt tgcctgagcc ggggtaacgt gtccatatat
    81481 tattggatcg gatccttcaa gatgaacctc tattgttggt tcaacatcgc atagaccaaa
    81541 acaacctgac tgaacgacgg agacatcgtc gattcccttt tcttcaagtg aatcaactat
    81601 tgccctaagc gtatcctttg cacctgcagc aattccacac gtacccatcg ccacaatcac
    81661 ttttcctctt ttgcctgagt tacgcatctt aaggcccttc attgcgttct ctttgatttt
    81721 cataagttcc tcgaggctct taatcttcgc cattactcgg ccctcctgta tttgtccaga
    81781 atcttggaaa cctcgtctgg gctgactctt ccgtagtaat catcttcgcc gatcaagaca
    81841 accggggcca tactgcaagc acccagacat ctgacagcat gaatcgaaaa aagcccatcg
    81901 gaggttggtt cgttcatctc aacattcagt tcgtcttgaa gtctttcgag gattttatct
    81961 gcacccttaa catagcaggc agttccgagg cagactttta tctgattttt cccctttggt
    82021 ttcatgctga agaagttata aaaagtaacg actccataaa cctgactcaa aggaatatgt
    82081 agtttctctg acacgtgttg ctggacttct tcggacaagt acccgaagat ctcctgtgct
    82141 ttgtggagta cattaattag tactcccgtg ttgcctttca ccgaatctat gtactcgtca
    82201 agctctttat agagctgctg gcattgcggg caattaacaa gagacaaacc atgcacctcc
    82261 tttgaggaaa gaatgttatt tatttcacac atgcattata acatgcacct agagaaatgg
    82321 gctgattcct gtgttaaaat aacccatgtt caaggagcct tatgaggagg agattagatg
    82381 gatttagaat cctttatccg tgatgttcca aactttccta aggaaggtgt agttttcaaa
    82441 gacgtcactc cattgttaaa ggatccgttg gcttttagag agtgcatatt aaagttgtcg
    82501 gaaaaagcaa tgaaagttga tttcaatata atggtagctc cggaagcacg aggtttcatt
    82561 tttgcggctc cccttgcttt tgagttaggg aaggcgcttg taccggtcag aaaaccgggg
    82621 aagcttccat ataaaacaaa ggaaatcgag tatgaattag agtatggtag agccactctt
    82681 caaatgcacg tagatgcagt caacagagga gacagagttc tcattgtaga tgatatcctg
    82741 gccacaggtg gaaccatgca ggcaattgct gaaatgatcg aagaaagcgg aggagaagtc
    82801 gttgaaattc tttgcctggc agaacttgga tttcttgaac ctagaaagag gctatccaaa
    82861 tacagtgtgg agactcttat tacttactaa tggaggtttg aagaatgctt aaatatgact
    82921 tctcatttgc gtttaaggaa acccttgaga gcgggttggc cttcgatgag atatttgatg
    82981 ttgggaaagc tttgtctgcc aactttgaaa aggtttttca gccgcttcct ggttttctta
    83041 ggattcttga cgatgacgag atccttgaag aagttggggc tttcaagtct tggcttcaga
    83101 ggttcgaaaa ctttgtcgtc ataggaatag gaggatcggc cttgggaaat caagcccttc
    83161 attcctcgct caaaccaatc aactggaact ctcttggtaa tgaaaagagg ggcggaaagg
    83221 ctcggatttt ccttctggac aacgtagatc cagacctgat agcctctgtt cttggagaac
    83281 ttgatcttgc tcacacggtc ttcaacgtaa tatcaaagtc gggaacaact gccgaaagta
    83341 tggccaacta tctgattgtc agaggtttac ttgaaaaact agaccttcct gtcaaagagc
    83401 atttcatatt tacgaccgac aaggataatg gtgttttaag acaaatcgca gacaatgaag
    83461 ggataagatc tctgacgatt ccagatgatg taggtgggcg tttcagcgtt cttacccccg
    83521 ttggattgct ttctgcagtc gctgagggaa tagatattga gcaaatctac gagggtgcaa
    83581 ggtttggcaa ggaaaggtac ctgagggatg atttgaaatc aaatccagct gctgtctgtg
    83641 ctctgattca ttacgcatac ttgaagaagg ggcacaatat ctcagtaatg atgccttatt
    83701 caaataggtt atacactctc gctgattggt ataggcaatt gtgggcagaa tctttgggaa
    83761 agaaattcga tctttccgga agaattgtcc atacgggtca aacccctgtg aaatctttgg
    83821 gcgcgatcga ccaacactct caagttcagc tttataatga agggccaaag gataagattg
    83881 ttacactggt taaggttgaa gacttcgtga atcaacttac aatacctttt atccattccg
    83941 atatcgaagc tctatcttat cttaatggaa tcggggttgg ggagctttta aatgcagaac
    84001 tcagggcaac ttctattgct ctcgctgcaa acggtgtgcc gaacatgacg attatcttcc
    84061 ctcagataga cgaagttcat gtaggcgagt ttattatatc ttatgagata cagacagcta
    84121 tcatggggtt tttgctgaaa gtaaatccat atgaccagcc aggagtggaa ctgggaaaga
    84181 aacttacata cgctcttatg gggagaaagg gttacgagga aataagggat agatatgagg
    84241 agaaaacctc atggaggttt gaactatgaa gtcagagcta ttaagagttc ttgagggttt
    84301 ttcagttgag gaagtcttct acacttcggg agaaccaata cctacctttg tgatagtttc
    84361 gatggaatct gaggatcttt tgaagaagat tggagaaatg gaggaaatcg aggcagacat
    84421 catagtgata agccctgaag agaagaaaga actgaagaat gcaagcagtg agttgtcaag
    84481 agtcgtgctg aatgttatcg agtcgggaga aaagcttctg taatgattgt tggtcttgtg
    84541 ggaaaagcgg ggagtggcaa gtcaacggct gcagagcgtc tcgccaaatc gaaaataaag
    84601 ttaatctccc tcgataagct tggacacgac gcgctgagag aagaaagaga aagcctggtt
    84661 gactcctttg ggagaggcat tttgacgtgc ggtaacgttg atagaaagaa gctgtccaga
    84721 atagtcttcg aaaactccga tcttcttaca aagctgaata agatagtcca tcctgtgata
    84781 aagcaaaaag ccctgcagat cgtcggtagg gatttttcag atcactatat ctttgacgga
    84841 gctcttatcc atgaaattgg actcgctgag tattgcgaca aaatcatatg gtttgaatgc
    84901 ccgaacgaga tagcgatcga gagacttatg aagagaggaa tgagcaaatc acgtgcagaa
    84961 tcgattctgg cctcacagca atacctcgat tcgataaaag agtctgtaga tgccgtagtc
    85021 actacattag gcagtattga agagactttc gagaaactga gatcgattct ttttgagtgg
    85081 ggcatagtgg tataattggg ttacttagaa attctttata aggaggtagg gaaatgcgca
    85141 agagactgat tctcgctttg ttgattgtat cttttgctgt ttttaccttc gctgtgacgg
    85201 agatcgaatt ctggcacgcg atgggcggcg cgcagggaac aacagtcaat gaaattgtag
    85261 catccttcaa cgaggccaac cctgatatct tggtgaaggc aatctatgtt ggtaactaca
    85321 gtgctttgca gcagaaactc ctggtaagtg cggaaagcaa gacattaccc gctctttctc
    85381 aggcttacgg gaactggact gccagactta tacctcgaga tgccgttcaa gaactgaatt
    85441 ccttgatttc cgatcccgaa gttggcttga cggatgagga atgggatgcc atatgggatc
    85501 ctttcaagaa aatggtcacc tggggtgata cagtatatgc ccttcctttt aacaaaagta
    85561 cttacgtcct ttattacaac actgacctct ttgagcagta tgggcttgag gttcctaaga
    85621 caatggacga tcttctgttt gcagcgatga tgctaactga ggacaagaac aacgacggtc
    85681 agattgatca atacggcttt ggtttcagaa ccacgatcga tcatttcatg atattcctga
    85741 gagccaatgg tggagaagca atcgatataa agcccgatgg ctcggttgaa gtaactatca
    85801 attctcctga agcaaaagag gctcttcagt tgatgtacga tatggcccat acctacaacg
    85861 ttgccctttt ccagggtggt tacctagacg caccattcgg ggacggccag atagcaatgt
    85921 ttgtagagac tattgcatct gcttcttacg ttgattccgg ttccagagga aagcacggct
    85981 gggcatgggc tcccgttcca atgtggaaag tccaggctcc tccttttgcc ggaacagatg
    86041 tcgtgatgtt caaagggatt tctcctgaac agaaagctgc tgcttggaag ttcatgaagt
    86101 atctcataag cccggaaata caggcttatt ggtcggttaa gacaggatat atgccggtta
    86161 ccgaaattgc tttgacaacc cctcaatgga aggcctacga ggaacagaac ccgactgtaa
    86221 tggttcctat tagtcagatt ccttacggtt cggttgatcc taatgttgct ctttgggatg
    86281 acgtaagaaa cattcttgga acaatggtag gagatgtaat caaccagaag agaaccgtgg
    86341 aagaagggct ggcatgggca gagcaggaaa ttctcttgaa agttgctgag caacaataaa
    86401 caaagctgtg agtagggagg cctatggcct cccttttttg ttgaattcag acgaatcaaa
    86461 gcaatggttc aggatccagt ttggaataat cagttcttga actcatgaac aagaacaaac
    86521 ggaatttcat ttcggttcgg agattatttt catcgactgt gtgtgtagtt ctaccctctt
    86581 aaggtagaat tatctcgaat gttttcaatt tccggagtac catccttgag aggcgggagt
    86641 aaatgaagaa gtactttcat gtattcaact tctacgcgtt ttcgttctca atactacttt
    86701 acttactaaa ttcatcgatc aacactaaag cggccgaatg gggtctgtcg tatatggtaa
    86761 ttggtttgat caactttatt ggctgtgttt tttacgtaat ctctgccctg gtattcggaa
    86821 gaatgggaga cagaactggc tttaagcgaa gtctgtcagt tggcatgttt atcatgacaa
    86881 tcgctctttt tcttggtttc ttctggagcc gacccgtaga tatagtgata gcagccattg
    86941 gggtgaatct cttttttgga ttctttttcc cgtcactaga agggttgctt tcgaagtcgg
    87001 aaaagagtat tggaattgat tccgcttcga ccgttgttcg ttttgtcctc tcgtggagtg
    87061 caggtaacat tgttggaatg gcttttgggc catttcttat ccagaggttc ccggcagcta
    87121 tcttttctta cggaattgct ctctgtggat tcggtagcat acatgtgaga tcacatctca
    87181 aaagacacgg cgaatccctt cccggttctt ttgccgaaac attcagttcg tcattcagag
    87241 agattgatct cccaaggcta gaggagttca gaaaagttta cagatttact ttcttgcttg
    87301 caggtataat ctacacaagt ggaatggcac tcttccctaa gctaatatct tcaacaggca
    87361 tacagctcca gaatgtgggt ttcttgacag taggtgcaaa tgtcggggtc ttcctttctt
    87421 ttgtcttcct gagcctcttt cgtttttggg ttggtagtcc tggactatcc ttcattacca
    87481 tgatatctgt ctttggagcg gccgttgcca catttttcct tccagagagt ccagtcacgt
    87541 tcttccttgc cactctgttt tcaggtgcaa cctatgcggt tccctacata tttgcaatct
    87601 tctatggtct tacctcgaag gaagacgatc atggtaagca gggaggcatt cacgagtcta
    87661 tggtaggtat tattttcggt gtcggaccgc tactgggagg
//
LOCUS       DAOQYI010000005        70827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_395_length_70827_cov_71.316750, whole genome shotgun sequence.
ACCESSION   DAOQYI010000005 DAOQYI010000000
VERSION     DAOQYI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 70827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70827
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_395_length_70827_cov_71.316750"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..2517)
                     /locus_tag="PKL93_03160"
     CDS             complement(46..2517)
                     /locus_tag="PKL93_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70293.1"
                     /translation="MRKALLICSLSLLIILAGCIKVPNTRIIKGTVFTDEYLSGAEVR
                     LLDVDGNQLLEEPHHTDNYGRFSVEIPLGTEFPLVILCSFDSSNDNEDKDLLASVVEE
                     SFRTELIPVNPLTSLFTAYMFKRGISYAEALQEIRGAFNIPDGVNLLSQIHPSFETSY
                     FSMKRLVSFVEEQFGGNYKAFVISLVEAIDEGTAFDFGVEPTLGLGISDILKELLKEI
                     GGTIYDKAKDKAFSWILKSLFGLGDGNEEEIKEILDQIREVNEKLDDVSSKLEVIDTE
                     IQEVLEKLDEQYYDTYIRTIDQDYFTPVEACQNKYLYITTLEATHSTDISIQELMEEI
                     NESKLDQVMLNIKKVIVGGTAETSYKSIFELYVTNALNLLRSGKHIDEAAKGGLGFYK
                     RCSSDSEIFTDYVNTYINLFKAMTTRQLYALNLLTEYEHNRGSNLAELHFENYNSYLA
                     AEVEEFWKWLEVFVTYAIGGDSLLIQPAYNADDIWFYNQADDAALKALGKDSGIVVRL
                     FWNSGETMDVSVDGEIPQGMKLAFGFLDKVYSMFNDSTGGVKLTLDNQQIQLMGVDEA
                     IEAASPLGTLHKFSFTYQDAEKSKEPGVIVRRYVFENLEDGRYSISRSTNSNVPGTSY
                     THTLFEDVHKNIVNDQLIAPEYLDESNYSLSINSESAQVQSLAISAYMPSMRINGAER
                     QITYGRSHNIADHDYVGVLSFPLNKYMKIEGHNYYEKTWILFIPEWSYSPFDAVVVDG
                     TNRETSGRYSCRLILTNKGDNAEGDNWGWQTGEVTRTHPVWLRATNQLSAACLAPYDL
                     SKKYSISKKDDQYKLPSTVFSLYPV"
     gene            complement(2949..4634)
                     /locus_tag="PKL93_03165"
     CDS             complement(2949..4634)
                     /locus_tag="PKL93_03165"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNQ70294.1"
                     /translation="MAGKYISCLFEDLGTTGVAVGVFKEREVVLKETFGLANEEQGIP
                     VKTDSVFHIGSVSKQFTAMCIALLEEKHLDVSRRISDFFPDLGSHASEIRVFDLIHMT
                     NGLPDLYKVSQYIMGLRESDYLTSTEAYNLLRNLRWLEFEPGKRWAYGNSGYFLLGRL
                     VEKITGSTLNEFATREIFSPLGMTNTFFREDNTRLIKNVVDGYCEYDYLHSKSFRLPL
                     DKSVRLSKALDLMECTGAGQLWSTLDDLLIWEKAFHKKMLGEDPERLSEKIISPVKLN
                     NGNRCDYGYGLFLTKRKGKTTVMHEGGSLGFNAAIYRIPAESLSVVVLANRNDFLHRM
                     LVKLGIEIYEAIADWVLGEDSTCLDSKDEGTKPEELSEDWLEEMKEGDKWFADRKSAQ
                     ICRLFVEEGFLKLDMNGEGVHILSPLRGLRFEEMDREFEGEIFRENGEKRLLLTNHSG
                     AVRFRPFEKRLSPCDLQEYCGRYYCRDIDTGYDLYATERGLLFSNTNAHHDAMNFEYR
                     PAVKDLFFTYAPPYLNCYFGVEFLRGRDQNVEAFVFRDYDNDGREFLKFARIH"
     gene            4686..4931
                     /locus_tag="PKL93_03170"
     CDS             4686..4931
                     /locus_tag="PKL93_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70295.1"
                     /translation="MRYYTLDHRIPNLPGQDEAFKRFSPVETGFFPVTDLFAEDASAT
                     GFANLSVGHAGGGTVSAEGIFSNHEGVQRRSDNRQLL"
     gene            5451..6623
                     /locus_tag="PKL93_03175"
     CDS             5451..6623
                     /locus_tag="PKL93_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ70296.1"
                     /translation="MKLTSIFIVVASLFYFMMGFATQSINSALPAIAVELEMNHSMTG
                     LVLGLPSLLYQVAVIVAAALSRKSGPFLSASLGISCITFSVIFMALSRTYYTFLIGRL
                     LFGFGLGATEISIALGVSQLAFKRTGGVLNSVYSFFALGSIVAPLVVSAVLTETNMWN
                     VPILIAAALFLVVVFLSWTVWKKAAKESLSDVEKRRITLPRDSMFWLVAAGGLLYVGY
                     EVGLFSWLSSFAYEEKLLPLRMASLTTSILALGLFVGRIVTGVVVDKFGLQRTLVVLG
                     TISLAGFSIAFFAGNSILLSLGVFITGLGFSGTFPTLQAIIVSGYRQNRETVLAIFAA
                     AGSVGAVTTNILVGGVSDRTNLAYGMSVIGVQIAAALLIFTSIYLIQRKRKKLATS"
     gene            6842..7306
                     /locus_tag="PKL93_03180"
     CDS             6842..7306
                     /locus_tag="PKL93_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70297.1"
                     /translation="MGIGIIIRCKECDYSKNLQIGVGMMYAPHNILNLESKSPILKSL
                     IPSKKTIEKIKMLIESKNAVLADDYGHYIYRCPGCSELFDRFFIHLDYDDESFEPSYR
                     CGKCRSTLERIDHNSDEGSIEERIGKILASFPCPKCGNRSLYVDSDCTLMWD"
     gene            7512..7984
                     /locus_tag="PKL93_03185"
                     /pseudo
     CDS             7512..7984
                     /locus_tag="PKL93_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
     gene            8547..8918
                     /locus_tag="PKL93_03190"
     CDS             8547..8918
                     /locus_tag="PKL93_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70298.1"
                     /translation="MFSFLLVIIYVAFVSLGLRDSLLGSAWPVMGKAFAVPLSFAGIV
                     SMIIAGRAILSSLLSDRLNRKLGTGLVTSLSVGMTAVALIGFSVANSFATICLWAIPF
                     VLGAGAVDAALNNYVALHFAS"
     gene            9027..9704
                     /locus_tag="PKL93_03195"
     CDS             9027..9704
                     /locus_tag="PKL93_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ70299.1"
                     /translation="MSVIQIVLTLVLFSGLCLWRSGKSERDGESKTSSKFLSVGKALR
                     IRGVLFVLIAFFVYCRPETTAGLWASSYLVEYQGVDVETEAIFSSLFSPGITVGRFLN
                     GFIVDRFGDKRVIRYGISTLTVGAILVGIPFKSEIFALGGLIVIGLGCANLSVIHATP
                     SNFGRENSQAIIGIQKVSTYVGTTFMPPLFGLIANGVNIGLYPVYLAVLAVLMPTITE
                     TLNLSIG"
     gene            9730..10575
                     /locus_tag="PKL93_03200"
     CDS             9730..10575
                     /locus_tag="PKL93_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNQ70300.1"
                     /translation="MLGAITGDVVGSRFEFNNHRDKNFELFTEDCFATDDSIMTLAVA
                     KAIMETEKSINKSGIKSRADSDYLSLLQMLAIKYMQEIGRDYPNCGYGGMFGRWVFSD
                     CPEPYNSFGNGAAMRISPVGFVAESEEEAVSLSGAVTSVTHNHREGIKGAEATVMAIF
                     LARHGLSKREIRSRISEDYYPLNFKIDEIRDTYEFSETCQETVPQAIECFLESVSFED
                     AVRIAVSLGGDSDTIAAITGAIAQAYYGVPDWIRLKTLSYLDRRLRFIYDEWEAFLDR
                     LGIVF"
     gene            10885..12534
                     /locus_tag="PKL93_03205"
     CDS             10885..12534
                     /locus_tag="PKL93_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70301.1"
                     /translation="MKRVLVILIVVFLVTSTVMFAADIKRGGTLRITSMKQGILIENF
                     NPFSPNSNEMAIGGFYETLIYFNPMTGKIMNWLAESYEWSDDLLELTFNLREGVEWND
                     GTPFTEKDVLFTVNLGKNNKALDVASLWSTGVVGVRSDKPMTVTFTFSDVNTTIIQRF
                     SSVFIVPEHIWSKVDDPLTWIGEGIPVGTGPFVLKEGSFSEQSFSVVRNPNYWQIGED
                     GKPLPYIDEVLTLTTTNEQVPLRLARGEFDWAGYFVANIDEVFVKADPEHFKYWLPEG
                     NLVFLNLNNLKWPFDNYNMRAAIAAAIDPVEITRIMASGAVPASLAGVKASYLRLAEK
                     GLNEYGIEYDPDYARYLIEKEGYKLNRNGIYEKDGKSLSLNLYVPTGWTDWIMGAEEV
                     SRQLKEIGIEAIVTLAAWPSPYKDMMYNGNYEMLFGIAVTGTSPYYQFDNWVHSKHWA
                     PIGENAGNYYGMRYKNERIDELLDSYKRLTDPEAQDAVMEEVLTIFLRDLPSVPMFFN
                     PVWFEYSTRNFVGWPNAENPYAEPRPTGMDKMPILLSIHLR"
     gene            12644..13624
                     /locus_tag="PKL93_03210"
     CDS             12644..13624
                     /locus_tag="PKL93_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70302.1"
                     /translation="MAYFARKIGLLLFALFVAVTLNFFIPRMMPGDPAQDLMDKMQGI
                     PLESLEAIKAAFGVDSTDPLLLQYGKYLLNLIKGDLGISISRFPTPVGRVLQVAVPWT
                     VGLMGTATIISFFLGTIIGIGVAWKRNTKLASFTVGLFIFVRSFPYFWLGLLLIYFLA
                     FRIPIFPLGNAYTPQLERGTLEFFLSVVKHAILPALTIVISSMGAWILTMRNNMINVL
                     AEDYITVAEAKGLPLRRIKTLYAAKNAILPSITGFAMSLGFVVGGGLLTEMVFSYPGV
                     GLMLYQAVQSKDYPLMQAIFLFISVAVLVANFIADIAVLILDPRVRDGGK"
     gene            13624..14601
                     /locus_tag="PKL93_03215"
     CDS             13624..14601
                     /locus_tag="PKL93_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70303.1"
                     /translation="MFRDVFSLFIENRKALIGLIILAFFSIVAIFAPLIAPYDPKTNK
                     VEVLSIVEEEIGRTKTVEREEIDEFTEKRVYTQSITTTYEKVTTKLPLNAKPSRNHLL
                     GTTRAGQDLFSQMVYGTRITLAVGLITGLFTTLLALTLALVAGYFGGIVDDIISLFTN
                     VFLVIPSLPLMIVIAAYITVKGVIPIVLILGLTSWPWPTRILRSQVVSLKNRDFVRVS
                     RILGERPLYIVFREILPNMISLVMASFFTSTMAAIMGEATLEFLGLGNVSVVSWGTIL
                     YWAQNSGALLSGAWWWFLPPGICIALIGTSFALMNFAIDEISNPKLRKR"
     gene            14606..15592
                     /locus_tag="PKL93_03220"
     CDS             14606..15592
                     /locus_tag="PKL93_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70304.1"
                     /translation="MNRQESEQLLQCRNLVAGYRIKSGFVHAVDDVSFDLERGGFLGI
                     AGESGCGKSTLAFAIMRLLKDNATIENGSLFFNGTDLVGLREEEMKKIRWVDISMAFQ
                     SAMNALNPVLSIGEQLTDVIHAHGEVRQSEARDRAIEALKLVDIPRDRFNSYPHQLSG
                     GMKQRVMIAMALILNPDLIIMDEPTTALDVVVQRTIIEKIQELRKSLGFSVIFITHDL
                     SLLVEISDTLAIMYAGKIVEYGNSDELFNMPLHPYTIGLMNSFPSLTGKITRMGGIEG
                     KPPDLLNPPEGCRFHPRCPHAMDICEKSCPALQKVDPRHSVACFLHKGAENL"
     gene            15589..16557
                     /locus_tag="PKL93_03225"
     CDS             15589..16557
                     /locus_tag="PKL93_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70305.1"
                     /translation="MSGDILVLEGVRKHFPVNSLRRHGKFVHAMDDISFSLRSGEVLA
                     IVGESGSGKTTTANVISRIYRQTEGSVVFEGRELSGKMTRTEELKYHKRVQMIFQDPF
                     GALNPTRTIGRIMERPFVIHRIAEDRKAVSEKVDSTLTQVGLEPPDQFKRKFPHELSG
                     GQRQRVNIARAFAVEPDLILADEPTSMLDVSNRMSIMNMMLSLKESHGVSFIYITHDL
                     AGARYMSDRIIVMYAGMIMEIGPAEEIITNSFHPYTKLLKSAAPAPETGLKRKPLITG
                     GDIPSLIDPPNGCRFHPRCPFAKEVCSLEIPPMKKIGDDHYARCVL"
     gene            complement(17108..19822)
                     /locus_tag="PKL93_03230"
     CDS             complement(17108..19822)
                     /locus_tag="PKL93_03230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ70306.1"
                     /translation="MSLRKKSISIKSIITIVSVSILLFSTIVIGYLIISRWLSSAEKE
                     AERIVENLGENIYNQVSSFMHIPHSVNEVNHKVIEYGILDLSNEKLREKFFVGVLSAQ
                     SPHIYSFSYGTANGEYYGALRNEKGVIEIMRNDASTGGNSWYYSVNEDLTAGERVVQA
                     GKFDPRTRAWYKAAEESGGPALSPLYKHFVMDDLTVSFSWPVYDASGSLQGVMGTHIL
                     LSDIGSFLVDAVRDYSGYAIVADKETGSLIANSMGVDNFTVLPDGSFSQRTVDDIGFP
                     DIEKAFIQSTTNRESPLFYRGEEEKLFINMREIEILNATWVVMSAIPEAFYMKDVIDS
                     IHLTVIIVVVALVLLLIIYHILARKLMKPLDNLIEVSTALSKGDLSKRVEVVRSDEIG
                     MISESLNRVADKLQFLINNLEESVIERTRQLQRANEELKESKNELQLLLDSTAEGIYG
                     IDLNGDCTFCNASSVKLLGFSGPEDILGKNVHRLIHHSRRDGTTFPIEECKIVRSIKH
                     GRGFQTDDEVFWKADGSSFDVEYRSYPQMRNGEVVGGVITFSDITERKKREEEIKYLS
                     SHDVLTGLLNRSSLAQIQKAVDVEENLPISVIVADINGLKMTNDVFGHAAGDELIKES
                     ARILLESCRERDLIARVGGDEFVVILPGTSEKETESIASRIKEGFSDARVQAVKCSIS
                     LGFDTKTRADQSLNEIMANAESFMYKDKTIHRKSLNRQLIDSIIDTLHTKYPMEKQHS
                     IAVSELCGEIGSAMKLSQSQINVLKRAGYLHDIGKIVLDESILSKDTYTALTEEEREK
                     IKQHSIVGYRILNLFDETLDIAEYTYSHHERWDGSGYPRGLKGEQIPLISRIISIAGA
                     YDRVMNRVEGTLEEKREAALKVIREDARKQFDPNIARLFIQMMDGKNA"
     gene            20397..20693
                     /locus_tag="PKL93_03235"
     CDS             20397..20693
                     /locus_tag="PKL93_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70307.1"
                     /translation="MGYSILSKLVFPSKELSLAIAFEKFDSYPFILRPSDEIALYLIA
                     AATQKKLPLLFISRAFGRNKMSRLVRHRNHVGCYGLFRFCIGLSTKRTHRSSAY"
     gene            complement(20869..21084)
                     /locus_tag="PKL93_03240"
     CDS             complement(20869..21084)
                     /locus_tag="PKL93_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70308.1"
                     /translation="MREERLSDAVGNVYKVKNTFDSMGAKELYEVFVAFQKTLAVEKG
                     EEKEIPALKKKFTLLLRRLLEEIGRLH"
     gene            21811..22692
                     /locus_tag="PKL93_03245"
     CDS             21811..22692
                     /locus_tag="PKL93_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ70309.1"
                     /translation="MKLLLTLMIVVTGTLCLAKGNYTLHSEIQYKSHEESLLNCLDIY
                     TPDELESKTGEIPVMIMIHGGAWRTGDKSNKNVTVNKVPYFISNNWIFVSINYRLSPE
                     VKHPAHVEDVAAAIAWVRENISSYGGNADKIFIMGHSAGAHLAALVSTDESYLARYGI
                     GLDAIKGVVLLDGAGYDICASMEKYGTGIIATSMYLNAFGQEEESWYDASPINHVAPG
                     KSIPPFLIVYAGERIKSEEISSDFALKLRLSEVPVRLYHAKDKNHADVNIDLGLVQDD
                     LTIVVADFLNDLLNSSE"
     gene            24240..24983
                     /locus_tag="PKL93_03250"
     CDS             24240..24983
                     /locus_tag="PKL93_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ70310.1"
                     /translation="MARRKSLYEIIYSQIEQKVQEMGDGTQLPSIEKLCEDYGASRTV
                     IREVITALEKEGLVVRRQGLGTFVVKDSGLVHTGIEYLRGLTRIISSSGKTPNLIHDR
                     FKEVLADGDLAAKLEMREKETLVLTERVYAADGIPAIFAKTYIASERIPGGTEELLKT
                     LGERRAKELVLFDLLEEKFKDPIRYAIAEIESRLVNEELADLLEMTPGTSIVLLKEVH
                     RDINNVPMLYSEDFINTSVFRIHVLRKKI"
     gene            complement(25023..25445)
                     /locus_tag="PKL93_03255"
     CDS             complement(25023..25445)
                     /locus_tag="PKL93_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70311.1"
                     /translation="MLREIMISFLLEGDACSSIKYRVQKEILEESQDTLNMRVLHSGI
                     LDDTSVKKIIENQKQDGWLGESFHGEDSMEASIRFLLERGLGSDDAVILRAFEALERD
                     SSDFSREFKKVGSVLDSRGFGGSESIRAALNVILKSSL"
     gene            25805..27295
                     /locus_tag="PKL93_03260"
     CDS             25805..27295
                     /locus_tag="PKL93_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HNQ70312.1"
                     /translation="MNLLLEKLKEVLLAVLPITVLVLVLNFLFVSLSWFLIFRFVIGS
                     FLIVVGLSAFLFGVSIGISPIGNFMGTFIAKTNKLWIVAVAGLVLGFFISIAEPALHI
                     HALQVELVSSGILSSGSVVITASIGIAVFLSLGLIRIVFNVALKKLMTVSYGIILILS
                     LFSSRELLAISFDASGATTGAMTVPFIVTLAAGISVLKRDSRSSENDSFGLLGIASAG
                     AIISVLLVGLVSTNNTMEAAPVYFASESSEILGYFFDLMPRIASEVLLSLLPILLAFL
                     LLQFSSLRLSKRMLRQITVGLLFTFVGMVIFLVGVNGGFMDVGREIGYGIASMGNKSI
                     LVLVGMILGLVTILAEPSVYVLTHQIEDVTSGAVKRRIVLLALSAGVGLAVALSMIRI
                     AVPAVQLWHFLLPGYAISIVLSFFSPKLFVGIAFDAGGVASGPMTGTFVIAFAQGAAS
                     AIEGANVLIDAFGLISMVAMTPIIALQILGLIYKIKTRKRGVESSG"
     gene            27288..27956
                     /locus_tag="PKL93_03265"
     CDS             27288..27956
                     /locus_tag="PKL93_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNQ70313.1"
                     /translation="MASNKNISPYKLIYAVVNFGKGSEVLRIARENGISGGTILLGRG
                     TVRSGLLNFFALYEIRKEIVLIVANNRTAKSLLEALDREMMLDRPDHGIAFTVDIQEV
                     EGSKELGCNSLQEGRGEKEIMYQLITVIVDRGKAEEVIDAATEMGSKGGTVINARGSG
                     VHERSTLFAMEIEPEKEIVMIVSKKELTQTIVNKIREKLRIDEPGKGIIFIQDVGDAY
                     GLLK"
     gene            complement(28043..28993)
                     /locus_tag="PKL93_03270"
     CDS             complement(28043..28993)
                     /locus_tag="PKL93_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70314.1"
                     /translation="MSGPAVKSKLASFLGKYKIIIAFFVLALVISLMTPNFLSWRNII
                     NIFRQSSIIGIMAIGSTFVIIGGGFDISVGSLLALTAAMAVGLQSSMHWFLAVVVVLL
                     VGAAFGAANGFLSSKIHIPPIIATLGTMTIIRGIVYMYTGGYPLYVDSEGFAFIGNGY
                     VGPIPFPIILLLVLVALGQFVLVKTKFGRYSCAIGGNKEAARLSGIKVDLYVTLTFVV
                     GGLMAAMSGIVYASRLLSVTPLAGQGYELDAIASAVIGGTSVSGGEGSVVRTLIGALL
                     LTMITNAFNLIGIDLYVQYVFKGLVILAAVGFDSFYKARG"
     gene            complement(28994..30544)
                     /locus_tag="PKL93_03275"
     CDS             complement(28994..30544)
                     /locus_tag="PKL93_03275"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNQ70315.1"
                     /translation="MIALNKDTVGPRLILEVKEISKSFPGVQALDRVDMDVREGEVHA
                     LLGENGSGKSTLTKCIVGAYQKDSGTILYDGKETSFSSPTESFRQGISVIYQERSLIP
                     KFTVEQNIFLGDEMQVSGLLSEKNMRKRFRSLCESYGFDLSPDEKIFRLGVAQQKVVE
                     IMKALSRNSRVVIMDEPTASLSKEESDHLFKIISQLKSKGISILYITHILEEVFRITD
                     RITVLRDGRKVSTLNTAETDREEIVNLMVGEVFHDIEDISSSANYDLPPVISVKNIQK
                     LPRVRDISFEVYPGEVLGITGLTGSGKTELARAIFGAEKADSGEIIVEGKRVNLSSPV
                     DAINAGIALIPEDRKSDGLIMLFEVFKNITLPSISDFTAMPGMILTKREYVKANEYRE
                     RLNIRLASIHQQTKFLSGGNQQKVVIAKWLLTNPKLLVMDEATQGIDVKAKSEIYSIV
                     RELAENGFSVIFISSEVPEVRRVSDRILVIADGEVVGEFKRGARQDEIMKKALSGKQE
                     HLDNQVVS"
     gene            complement(30528..31739)
                     /locus_tag="PKL93_03280"
     CDS             complement(30528..31739)
                     /locus_tag="PKL93_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70316.1"
                     /translation="MHEAGKARIFSQLFLNEEFTEVNSRLLKDKLTIAKKSGLERLMI
                     WSVEPRFLRPVVSICRGAGVSPHLWYPLLADTPVNFDTSAFEVTGIIERSESEILDYQ
                     KAGGEEFKFLCPNRVGEAPAFLDRFEEIINLADFDGVFLDRIRFPSPANGIGEMLTCF
                     CDTCKVKSHGLVSDFEKTFCAFIEELNTERISSEVLRTITEFRKTSTGFREFRISSVL
                     NLLSEYSRKAREKGLEVGVDLFSPSLADFVSQDYGQISREVDWIKPMIYCKTMGPAGL
                     PMELLSLAKTLMKLVPEFSEKVALKIISEFVGLAIPGSFDELIADGIDQSNFSLELDK
                     IVRMSLSPSVKVFPGFEAVNLPPVCSVGLEEVRNYVDATLRKGHDGFVLSWDIRHISD
                     AILEYVGDRFE"
     gene            complement(31763..32689)
                     /locus_tag="PKL93_03285"
     CDS             complement(31763..32689)
                     /locus_tag="PKL93_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ70317.1"
                     /translation="MKKLSVLLVIVFGLVTGLLFATDVAVLLPGTVEFFSVQRIGLDK
                     AAKEFDLNLIYADAEWDAGKQLSQVENFIARGVDLVLLCAADNMALRTAVTRCNEADI
                     PIITFTNSIGTDPEGKYPGVVSHIGRSEIGSGVVQAEFVEKLFGNVELDIILIEGNPG
                     TTAQRMREEGFLSVAERNPNWRIIEKRPIDGWTKEGTLAFMEAFLQSERNVDVIACQW
                     WSGAIAASMALKERGITDVVVLGLEYAKELVPYIEAGDVYASTYFSVIEEGYRAVEAA
                     HLYLTGQEVPKFIEIQQVMVTKDNVADFEPEM"
     gene            complement(32720..33886)
                     /locus_tag="PKL93_03290"
     CDS             complement(32720..33886)
                     /locus_tag="PKL93_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNQ70318.1"
                     /translation="MVSRLIESRTDEILRIVRGRESTSRVQLAKLTGLSKPTISSIVN
                     ALVAEGVITENGLGKSKSSGGRKPINISFAKDYRYVLSIDIGGTKTIFALIDLDGNIT
                     NSETIPSDALRTRRGLIDELSERIESYIAQVDRGKVLGISIGIPGTVDRNTQNIKYMP
                     SFDMGNFDLKSHVEECFGIPTLIENDVTLAAFGESWIGSAKEFGDVVLVSIGTGIGSG
                     IVIADSVYRGSSGGAGEIGEFVTDWSIESKMNIGFGRLEQWFSGYALESFCRSNGMKV
                     SVKELFENMESDDRISKRIVSGCEHLALAFANAIMLLDPARLLIGGGIGFNQYDRIFP
                     IINDTLRRVLPEELYRSDLLARASLEPYSVVIGGAYFAQKELLLKEVLGGASGF"
     gene            34543..35286
                     /locus_tag="PKL93_03295"
     CDS             34543..35286
                     /locus_tag="PKL93_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ70319.1"
                     /translation="MARRKSLYEIIYSQIEQKVQEMGDGTQLPSIEKLCEDYGASRTV
                     IREVITALEKEGLVVRRQGLGTFVVKDSGLVHTGIEYLRGLTRIISSSGKTPNLIHDR
                     FKEVLADGDLAAKLEMREKETLVLTERVYAADGIPAIFAKTYIASERIPGGTEELLKT
                     LGERRAKELVLFDLLEEKFKDPIRYAIAEIESRLVNEELADLLEMTPGTSIVLLKEVH
                     RDINNVPMLYSEDFINTSVFRIHVLRKKI"
     gene            35299..36084
                     /locus_tag="PKL93_03300"
     CDS             35299..36084
                     /locus_tag="PKL93_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNQ70320.1"
                     /translation="MEDYREPVGLEGRFQYHIECRAGDIAPVVIVPGDQGRVDKIVAG
                     LSETRKIAENRGLITYTGKYKDYPVSVTSTGMGGPSASIVYEELINVGARVLIRIGSV
                     AGLQEHVNEGDIVVPYGCVRDDGASNYYVPANFPAVPSPDVYSSLTTSARERGREIVT
                     GINWTHSCFYKRDPEYFQSWSRRRIVSLEMEASALFVISYLRNVKAGFIGICYANRYK
                     QSAGTKVDLSVKNPRRDVIENSVKEAIEITLTAIKKMYDEDLV"
     gene            36106..37386
                     /locus_tag="PKL93_03305"
     CDS             36106..37386
                     /locus_tag="PKL93_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ70321.1"
                     /translation="MRTRFMLLLFVLLIASCFAVPLRLSSEVGIHTDAWKTRMEGFTK
                     ETGIEVDIQQFPYANYLDQLMLGYTSGRVEIDVPYISMLWYPALSIANYIYPISDIPG
                     YEKINEADIPGIRNAKLNGKTYIVPYMNELGGIIYRKDLFEDPVEKANFLNKYGYELL
                     PPQTLEQYRDVAEFFNRPPDLYGVTMMGRRSIFLATHFMQRLWAKGGALLDMNMRPIF
                     NSEAGVEALEEVKYMFQFANPAAMNYDFQEALNEFISGRSAMAEVWTTGMFYVEDESR
                     SSIVGKAGFVGFPRPEEKLGEKLPMLYISWGFSVSSSAPDKEAALEWLLYVTETKNEV
                     EAAPTGNIPARFSALNSPELAESFPWIGDFASAMENCIPTPIVPLIPEGGSIVSGIIA
                     PAVSEFLAGTKTAEKALDDAVKEVDRLMRDGGYY"
     gene            37440..38327
                     /locus_tag="PKL93_03310"
     CDS             37440..38327
                     /locus_tag="PKL93_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70322.1"
                     /translation="MKSKQKTKGFAYILPALVFIVVIFMIPLTYTVVMSFLRWNLLRP
                     DLGIKPAGFTNYVRLFTDSFTLDTVGRTFYFVVGAVLVELIIGLCIALALDTEFKGWK
                     IVQSILLVPFMIAPVVVGYVWRFVLNSDYGPIIHLMKQIGFGGLVDKPLLSNPSAAMP
                     VLIVADAWEYIPFVTLVLLAGLKSVPYEPYEAAFVDGASSIQRFFYITLPLLRPSILV
                     AVVIRTLTSLRVFDIVFIMTGGGPGTATETLAFYGYRTAFQAYNVGFSSAINMLTFGI
                     AVVFTILYMKIIGGGKNYD"
     gene            38320..39147
                     /locus_tag="PKL93_03315"
     CDS             38320..39147
                     /locus_tag="PKL93_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70323.1"
                     /translation="MIRRRPVTRILVFAVIALALIWTLVPLAWILLSSFKFEADQFSI
                     PPKWFPERLTLQNYAKFFGNSEFVRSLINSVMITFLSTVIALFLGVPAAYAIARFNWK
                     HANTIAFVVLIARMTPPIVMVLPFFMIARYLGISNTYLPIVLALSFFSVPFAIWMMRG
                     FFQEIPVSLEEAAMIDGCTRFQSLRRVVLPLVMPGLSATSILCALIAWNEFLFALVLT
                     GQDTRPLPVLVNMFVSERNIEWGVMSAAAIITVLPMIVFGLLVQNDLVRGLTMGSSK"
     gene            39311..40366
                     /locus_tag="PKL93_03320"
     CDS             39311..40366
                     /locus_tag="PKL93_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNQ70324.1"
                     /translation="MPRMLVATAPRKAAIVEYQDRKIAANEVMVEVEFASPKHGSEVA
                     DFRSESPLIDEFYDQDWHLILPRSAGEKKGVEFGSWNLGNMWVGKIVVKGEDVKDYSI
                     GERVCSYGGIRETHIINAIGNDRLLKVPTGVSWKSAVCYDPAQFAFSGIRDGNVRPGD
                     YIAVIGLGAIGLMAVQLGKKIGARPVIGVDPIAIRREAALNSGADYVLDPGECDIGLE
                     IKKLTGKLGADCIIETSGSAGALQSALRGLAYGGTVSYVAWAREFAGGINFGREAHYN
                     YGRVVFSRAASEPSPDFPRWDRKRIEKTCWNLILDGSLSGDDIVDPVVNFEESPSAYC
                     KYVDTNPELSIKLGVAF"
     gene            40378..41184
                     /locus_tag="PKL93_03325"
     CDS             40378..41184
                     /locus_tag="PKL93_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ70325.1"
                     /translation="MKVITQDKDFFPSQMKEKLQFIRSLGFDGYEIDGKDLLERFAEV
                     SEAVRSTGFPVTSACGGYRGWIGDFSREKREQAVEDIGEILKALSKVGGKGIVVPAAW
                     GMFSKRLPPMVPPRSDSEDTAVLLESLESLNKMAAETDTFIYLEPLNRYEDHMLNTLQ
                     SAAKLIDVGGFSNVRLTADAFHMNIEEADIRQAVIENGRYIGHVHIADSNRYQPGQAH
                     LDFKTLFSALSEIGYSGDIAFECRVIGDNPAKRYGESCAFIRDLLRSIER"
     gene            41181..42227
                     /locus_tag="PKL93_03330"
     CDS             41181..42227
                     /locus_tag="PKL93_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ70326.1"
                     /translation="MKYGKLRVALIGAGQIAEVTHLPVLSQMNDRVELVGIADPAVER
                     AVMLSSRFGIDNTFGDYDEMLSSLNPDAVFVCTPNKFHSSATVSALRSGSHVFCEKPP
                     AINAREVQEMMVASKEAGKILSFNFHYRFRSEAIAIKKFVDSGDLGEIYFCEATALRR
                     RGIPGWGSFTNKEIQGGGPLVDIGVHMLDLALHMMKFPQPKSVTASAYCKIAKRNGVG
                     LMGGWDPEKYSVEDSAFGFVRFHNGASLILKTSFALNMKDRSIMNLQIFGDRAGATVF
                     PPEVFAESHGELTNTAIPYAESKDCYKRSIESFIDSCLGEESRISSPEEALVVQSILD
                     AFYLSAESDSTIEL"
     gene            complement(43044..45656)
                     /locus_tag="PKL93_03335"
     CDS             complement(43044..45656)
                     /locus_tag="PKL93_03335"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HNQ70327.1"
                     /translation="MAWYSLELEEVLEKLNADPNKGLSSSEVEERLARFGSNTLREER
                     KKTIFERFLEQFKDVMIVILMAAAAISFVVSLFENEGFFEPVLIMLIVVLNAVIGVIQ
                     ESKAEKALDALKKLSSPNAKVIRDGEQRIVQASNVVPGDIVLVEAGDFVPADARILSS
                     ASLKVDESALTGESVSSEKKTEAKVKEDATLGDRFNMIFSGCSVTYGRAKAVVVETGM
                     QTEMGKIASMLSAEEETETPLQKKLTVLGKYLAIAAIVTCAIIFLVGLIDGIPIMEIF
                     MISVSLAVSAIPEGLPAIVTVVLAIGVQRMVKRNAIIRKLPAVETLGSATVICSDKTG
                     TLTQNKMTLVKAFSDCNSALEEISSENSPEIRSLLLYATLCSDGRIIYESGEEKQIGD
                     PTETAIVFAAKRNGIEKEKMEEKYPRLAEIPFDSERKMMTTINRIENKNVVITKGAID
                     SVALRCVAGDVEKGRRIAEEMGSDALRVLAIAYKHIEEIPEFLDPAEIESGLTFMGLV
                     GMIDPARPEAKESVALCKQAGIKPVMITGDHKLTAAAIAREVGILSPEEKVVDGSELA
                     KMPDSELEERVRDISVYARVSPSDKLRIVRAWQKQGEVVAMTGDGVNDAPALKAADIG
                     CAMGITGTDVAKSAADMTLTDDNFSTIVEAVKEGRGIYANIRKVVGFLLGTNIGEILT
                     VFTAMIIWREAPLISVQLLWINLVTDSLPAIALGMEPVEEDVMKSKPKRKNEGIFANG
                     LGYRVILQGIMFAFLTLIGFSIGWKSTGDIVVGRTMAFAILSLTQIFHSFNMRSNHSL
                     FKTGFFSNVFHIFATVVSLAMVAFVLFIDPVARIFGLAKVNASMYLLAFLLSLVPIPV
                     IELEKLAGVIRHRD"
     gene            complement(45761..46489)
                     /locus_tag="PKL93_03340"
     CDS             complement(45761..46489)
                     /locus_tag="PKL93_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ70328.1"
                     /translation="MKSKDANDARERIIDASIELFSKRGFHATTTSEIAEAAGVTKGL
                     IYYYFKSKEEILSYVVNSLLEDATSITMDFVQEGIIQMIKDGELDIEYDRMKFSNEES
                     AIVFVNRLLEYYERILDYLLGNRLLLRVLMFESLKDSKHHNDLFRLLDLSRDIDSNPI
                     LKLISEADHDFTYSLDMELFKFFFSVMPLVTFAAYYDDLKERSMMSGDELRNSFLQSF
                     RVVLSSLKSGNEISLRTKRSDDTE"
     gene            47591..48625
                     /locus_tag="PKL93_03345"
     CDS             47591..48625
                     /locus_tag="PKL93_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ70329.1"
                     /translation="MEHFLLEELKSGKYSVGDKLPTEKELMKKFSASRETVRKALDRL
                     SFEAAIVRRPGLGTFVGYGSDNHLVGILVQQITSYIFPYIVLGAEDHLFRNEYKMLLG
                     NSAEDPVKERQILSEWIASGVKGLIIDPVYSATKRSNKDLVKSMAKSGIKIVLVHSDW
                     NIDQVSCNVLDDAFGGEKAAEIFFEYGHKRVAVIYKSTHLPGVIRAKSFVDRCRQLGF
                     SKIYEKSFNVSEFTGAPMQIAHELMSLPLQIRPTAIFCYNDATALQLQLVAKRLALNI
                     PEDISVIGFDDGPIGDFRDILTTFAHPKEDVGRRSVEILLDMLNGSKAYRVVHKPELI
                     ERSSVAGVKE"
     gene            49938..51167
                     /locus_tag="PKL93_03350"
     CDS             49938..51167
                     /locus_tag="PKL93_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ70330.1"
                     /translation="MRKLMVMVLALVLAVGFSFGITITMMSPLTGADGAYMDEIVARF
                     NEEHSDIEVVHVVVESSLDMKNKLSMGIASKTAPEIMFIRKFDMPLYLEHFKGFTKEE
                     WLENYGIDTDDIFPGVLEGLVVDGKVVGIPLDIWLFYMAYNKANFAKVGLDPNNPPKT
                     REEFISAMEALLPITPEGLTPYYENPAWTWIWFHLLWQHGGEVLTDDFKAPAFEKAGI
                     EVCKLMLEMQDRGILPMQVADPGVSFQSGESSVLITGIWTIKPWMEQLGEDFGYAVAP
                     QLGNTKAVFGGSHVLAMPAVMVEDPKVLEAATTFIKYLWDHAIDWYEAGQTPARKSIA
                     ESEELKERLPHIYAVSQDSEYVRQFQMFPLISEIEAEIAVYLEEILVLHSMSPEAGMK
                     EAAFAVQEILDDYWSRN"
     gene            51211..52122
                     /locus_tag="PKL93_03355"
     CDS             51211..52122
                     /locus_tag="PKL93_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70331.1"
                     /translation="MRQSHSKSPRSFRREVGSWLFLLPHLFFFILFVAVPLVFGLIIS
                     FFNWSLLKENVFIGFNNYVRTWNDSRFWPTVQHTVIFALISVPLVIIVAILFAHILKK
                     KRFGQLWLLVAFVSPAFFGSVGILSSWKWIFASFPSGLANYYLDKVGLINNAVSWFGS
                     TSVAWGVIILVTVWWIVGFSILLYLGALQRIPPEQYESAKLDGAGPWKRFIYITLPWI
                     KSVLFFDVVRQAILAFGLFDQAYILSGGGPAGTTRTLVYYLYLVGFERQDFGRAAAIS
                     WYIFAIVLIFAVIQLVLVTKSIRSTEG"
     gene            52127..53005
                     /locus_tag="PKL93_03360"
     CDS             52127..53005
                     /locus_tag="PKL93_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70332.1"
                     /translation="MTSTVPKNKKKLSKRITAVIVTVLLWAFAAFWFAPIFWMLSTSM
                     KSTAAAVIETPPQWIPQNPTLENYKIIFAPSGGLSLIRATLNSVIVAVSSTLATLVLS
                     IPAAYALSRLRFRGRMLIFWIYVAVLAFPAVLFLIPHFFIINGMGLSNSYLSLILPGL
                     GGTFGVFLLRQYMLDIPRELEDAAWIDGCSKFRFLISIVIPYIKPAMLVLSLMTFLGS
                     WNSFLWPLLILSKADKFTLPIALIRFSAGWGDPYRGIGPMMAGAFFSVAPSLIIFIVF
                     HRYLMKGISLGSLGKE"
     gene            53011..54426
                     /locus_tag="PKL93_03365"
     CDS             53011..54426
                     /locus_tag="PKL93_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNQ70333.1"
                     /translation="MSGIKKCFRKTEGESFGTPLERYFVLILGLLFLSLCFGGSVLPI
                     DIDISAFEERIEANRKVPIKIEIVDENGHPVSDAHVSVRQMNHEFFFGNAPEYLIYAY
                     AWTSYNRGRRFGIKPLPEEDLEVYKRLYLDLFNFATLPSFYWKDYEPTQGRIRLDEAS
                     KKIAEWLNENGVPVKGHTLVWGNPPSVGVPAWIEVKGKLGQWAEVREALFFRVEREVE
                     EFKDLVQYWDVVNEPVVQSWFDALGPDYIAESFRIVKETDPDAITVLNEYGVLVNERT
                     RRAFISRARQLIGDGVPIDVIGAEAHIFTAQDLLSQLQSLESIYLAIDELAQLGKPIH
                     ISEFQIPLPAVIDAFDVSINEAEEIQAEIAKIFYTVFFSHPAVEVITYWNFYRAWQSG
                     SGFLRDDLSEKPMFYELMKLIHGDWKTSFEAELDSSGELCFSGFSGEYDITVEFENFS
                     ETVSINVKKSENNQFTLVLGQ"
     gene            54443..56389
                     /locus_tag="PKL93_03370"
     CDS             54443..56389
                     /locus_tag="PKL93_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ70334.1"
                     /translation="MRSTTIILTLVFLAVSLSTSALANPDHVLKVDPSSLGPEVSSLL
                     YGIFFEDINHAVDGGLYAEMIRNRSFEHQDPVEGWFAVFESDCKATFSIETDRPLNKN
                     NTKYLSFEIQSDDKLISLSNDGYDGIPLKKGERYLFSVFVRSDEEYNGEIKVMLVDTK
                     GEPVAEKSLGTAFSSEWEKYSVELQPNESLDDGRLIITMKGSGKISFDMVSLFPEENW
                     NGMRIDLLEMLEELKPAFVRFPGGCLVEGDSLENAYRWKDTIGPIEERKTNYNLWGYH
                     QSYGIGFYEYLLLSEHLGAEPIPIFNAGMSCQVRGAEYCPMDEMDEWVQNVLDFIEFA
                     NGPSDSLWDSKRSELGHPEPFNVKYIGIGNENWGTEYHDRFPLFREAVKEKYPEITII
                     FSGPPSYEGASFNRAWRWARSNGIEIFDEHMYAVPEWMLRNTDRYDKYDRSGPKVMLG
                     EYAAHAAGTRNTLQAAIAEAALMTGLERNSDIVIMAAYAPLFNREGWSQWTPDLIWFD
                     NTRVYGTPSYHVQKLFNDNTGDVIIPSSLTDEDIEIIGYWFKSLYHVCSYDYESKELI
                     IKIVNPWPEEKKLRIEIGEEVQITGKAEMISITSENIFDENTFQNPDKVRPKTVGMSD
                     LSNEFTLTFSGNSVTVLRLETTGL"
     gene            56419..57903
                     /locus_tag="PKL93_03375"
     CDS             56419..57903
                     /locus_tag="PKL93_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ70335.1"
                     /translation="MQTSVKVWPRKLGKINEMIYGHFAEHLGRCIYDGIYNEKSPKSD
                     SRGFRKDIMEAIKRIKCPILRWPGGNFVSAYHWENGIGPLEKRPQLPNYIWGGVESNR
                     FGTDEFIQYCREIGTEPYLAVSLGTATLDEAISWLEYCNLDTPTKYSRMRRENGHPEP
                     YGVKYWGIGNEVYGPWQVGHCSAAEYAEKLRQYTQFMKGVDRSIKVIAVGADDPNWDM
                     TVLKHAGHLIDYISIHEYHGSDDYLGTVASVYYCEQRLRLLEALIKHLRLDHVKISFD
                     EWNVWYKISAEKESMDKGIVLLEEPYALKDGLFAAGIFTVMHRMADTVHMANLAQMVN
                     ALGMIKTKGDEMVLTPIYHAFDLFVNHTGRTRLNMAVDCDTYDINTRTFVEGRNSFKL
                     SGVPYVDGSATYDEEKQVLSVALINYYQKEEEVRLDLSGIGIKKKAVLNVLTGSAPDE
                     MNDFDNPDRIKLIKKELDNCSTEMLLKLDPMSANVIEFSLESSL"
     gene            58008..59531
                     /locus_tag="PKL93_03380"
     CDS             58008..59531
                     /locus_tag="PKL93_03380"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNQ70336.1"
                     /translation="MYLIGCDIGTQGTKSVLVNERGEVLVEAQREYDVIKPGSNWAEQ
                     WPDVWAKAAFNTIREVAEKAEVAKKEIAGLAISGLYGGSGVPVDREMKPLHPCLIWMD
                     RRATAQTSWVKENIPKEKIFSITGNYVDSYYGFTKIMWLRDNRPEVWKKTYEFVTPKD
                     YVVYLLTGEDAIDYSSAGNIGGVFDIRKLTWSREMCEALGIPMGLLPEKIVKSSDIVG
                     RITAESSKLCGLLEGTPVVSGGIDAPVAQLSAGALEEGEHVAMVGTSTCWGTVHDGKE
                     MPFGLVSFPYVVHDTERIYSFGGSATTGALAKWFKDEFADSEESVGKRTGTSPYQLLD
                     REVEKIPAGSDGLIVLPYFMGERSPIWDPFAKGVFFGVTLVHTRAHIYKALMEGAAYA
                     LRHNMENGIKAGLKLDDECWIVGGVAKSTVWNKIFADITGYKMRQVSSLVEAPFGDAF
                     LAGLGVGLIDKPERIKDWVKFRDPISPDPNHREIYEKQYSLFRELYEKTKETMWKMV"
     gene            60276..61802
                     /gene="araA"
                     /locus_tag="PKL93_03385"
     CDS             60276..61802
                     /gene="araA"
                     /locus_tag="PKL93_03385"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HNQ70337.1"
                     /translation="MRKGGLIIISTDSYEVWFLTGSQELYGKEVLRKVEENSKKLVNA
                     LNEKITAPAKIMWKPVLKDAIQIKATIDEANSNNRCIGLIAWMHTFSPAKMWIAGLKS
                     VHKPFLHLHTQFDQKIPWDTIDMDYMNTNQSAHGGREFGFISTRLEMKRKVIAGHWTD
                     KTVISKLEDWLRAALGWNEAHRLKVARLGDNMRDVAVTEGNKVDAQIDFGYEIHGFAV
                     GDYIEGYFKKVKRSEAEELVKEYLKEYPVVKSTVSEEEFQSSVREAARIEISLRRFLE
                     DGGFKAFTTTFEDLHGMKQLPGIAVQRLMRDGYGFGGEGDWKSAALVRIMKVMAEGID
                     KGTSFIEDYVYHFEPGNVKELGAHMLEVCESIADGTPSLEVHPLSIGGKEPPTRLVFN
                     AKAGPAVQACIVEFGDRFRMIVNELDVVKQEVAMPKLPVARVLWIPKPDLSTAATAWI
                     LAGGAHHSSMSYTVTTEQLEDLCEMFGIEIVVIDENTDIREFKKELRYNEIYYKLKKL
                     "
     gene            61854..62543
                     /locus_tag="PKL93_03390"
     CDS             61854..62543
                     /locus_tag="PKL93_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HNQ70338.1"
                     /translation="MLNKLKEEVYKANIAIDEYGLVKLTWGNASAISREEGLVVIKPS
                     GVDYSMLSPDDMVVVDLEERVVEGRLKPSSDTPTHIFLYRSFREIGGIVHTHSTWATA
                     WAQACKSIPCFGTTHADHFYKEVPCTRFLKTEEVENDYELNTGKIIGETFSTRDYQRT
                     PGVLVAGHAPFTWGKDVKDAVKNSIILEEIAKMACLTLLIEGETEALPEYIMNKHYFR
                     KHGKDAYYGQK"
     gene            complement(62680..63288)
                     /locus_tag="PKL93_03395"
     CDS             complement(62680..63288)
                     /locus_tag="PKL93_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNQ70339.1"
                     /translation="MADFKTHICWGIASYPIFILLSLSAIELLPVQQLQDSRLIGSGY
                     LLYVLGSDLPDIDSKSALIKRIIEILIASYVASALYISIISPRVQPFLLSRLPSLPVA
                     VTLSFSLAIISGLVVSKLTNLLSHRGFFHTVWGSLLYGLVIESVVVFRLESFGGSMNH
                     TETIFLGIAGSSGYCLHLLLDKIHGVKRTRRSDPGQESNSIL"
     gene            complement(63281..63787)
                     /locus_tag="PKL93_03400"
     CDS             complement(63281..63787)
                     /locus_tag="PKL93_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70340.1"
                     /translation="MARQTLKMFYPISIKNIRKLKAGDLIEYTGQVLLADETTVDKYR
                     TYERLEGTPFYNLSRELILFCTPSEGGLSSDLVKIDLDDLEYLFMSGITATVGLNVED
                     EEITALYKRFSRVNLSLCNRTSLYPVDLNEPVPEEVECGMSISLKDVLLYVDISSRGT
                     KFGSEEDG"
     gene            complement(63798..64667)
                     /locus_tag="PKL93_03405"
     CDS             complement(63798..64667)
                     /locus_tag="PKL93_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4895 domain-containing protein"
                     /protein_id="HNQ70341.1"
                     /translation="MTYYSGPESYREILRRVYEKVEDSFVSWAKLVKDHSDRLDNAFG
                     HFMTLVVQTSKGNAPVLSVFVDSEGRGYVGLSNSSPFETASALYRFPNEVENPYMEVF
                     AGFFGDETELMYHRAIFQSPLKLYFLAYYGNERLLRKEILKDSLRGKDYFRLSEVIDD
                     TLFSICRENYRKWIEFDDGEVLVFPFQNILKIAFGLPKINENIDRSIIMELSRLFRIE
                     VTKQCDVLRNSSVTPDMNISRPVATVFEIDLVDSKPVYERLESFYKYYSKFVSETIES
                     MLRFIHTDFPLSK"
     gene            complement(64664..65857)
                     /locus_tag="PKL93_03410"
     CDS             complement(64664..65857)
                     /locus_tag="PKL93_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HNQ70342.1"
                     /translation="MKNEELMERAVFELGKDSPFYNFLLLFIDRIPSVAVRTLKLRVS
                     SRGRFQLLYNPDTLRNRPLTFTKALLKHECLHIVNGHILIPVSKSREKTLWDLSMDAA
                     VNQFIRELDAFSLPMDSILKEGCGTDNERFFVGPPMQYPGMTAEFYHDWGLDFMKKNK
                     TIDLELLNSNISRPDSHEDFGKFELPKEFVEDLLKQVISETMEKAKTGMPEGLEGVMK
                     LVLDNPVLDWRNMIRRFFGSSMQVGRYRTPMRPNRRYDDQPGWRNDYAAKLVVVVDTS
                     GSIIEEEFNSFFSEIDEVTRVTDSRVWLVQVDETVQSVMKYGKGMWRDLKLMGRGETD
                     LQPAVDYAQEKLRPEGLLLFTDGFTDLPTVSRRVLFVLPKSHNSEFAEEAKSIYGRSS
                     VVFLK"
     gene            complement(65847..66944)
                     /locus_tag="PKL93_03415"
     CDS             complement(65847..66944)
                     /locus_tag="PKL93_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNQ70343.1"
                     /translation="MKVQDAKHLSKIIMESGEIPLLWGHFGVGKTDLAREIASETGRE
                     LIILVISQMEPGDLIGMPSRDGERTLFLRPDWWPTEGNVILMIDEVNRAHRSIRNAIM
                     QLLIDRRIHNHFLPEGSWIMAAANPPDEEYDQVELITDPAFMSRFFHIELTPDPDEWL
                     KWAEIQGMPSNVTNFIREYPEFLSRDTIVSMRLELRPSPRSWYKLGRVFADLSSEEIE
                     RYGYVIAAGIVGPEAARTFMSKIQGSELLPSPRALLLDLDENILGRLSEGDISWTSAS
                     VLRVTKHLSELDDLQVQEYFQNVPVIAGNLLRIGKVIPKDSFFSVIRHIVHQVEGEKG
                     LKRAFYENLLEELAMDHDVLKFLQRSEKDEK"
     gene            complement(66968..68548)
                     /locus_tag="PKL93_03420"
     CDS             complement(66968..68548)
                     /locus_tag="PKL93_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70344.1"
                     /translation="MLTRQGKRAFVASFLAVAFVIMIAVPIAFYLRGYLVPNFSRVAD
                     LSDQLRELTGIDLKLIVISFFSAIIFSILLGSDLPIVVSNLFFSERVEMLLNLPVRTA
                     TIANVQLLEVLAAGGLPILLFAPVFMAALRGLGYSDGRFWMALVLLFVFVLNTLAITA
                     IISFVIVFLSKGRFLKTLSAMMTMVTLFVFIFTLRFMDFSAIDLAKPDQVAEQFGGLQ
                     SVVTSPYLPWSPFVRAITGDTRDLAVFIVSSIGIFSSLALLEIFLYSKVLQKLMTRPI
                     GNVSTKSNGDFKVHGLFLGLLIKDSLLLLREPKLMFAILYPSLFVPVVVLVNPGLLSS
                     FGILQLLGLIVFLFSNYTTVSSTALFAFERQLGDTSWLFPIRRGKTILSKSFVITSLY
                     SFVILLVGLYVSNNTSQYRAFMMNFILFMIPTIFVLSLLGGFLEKSFGTKDSKNVFKA
                     LSLSGAILSFLLSTLLPVFSTLPMTLYISGNIGLFLNFMNIPIVSPFTWLLGLVLPVS
                     LWVSIAWASFRSLCYNFV"
     gene            69117..>69362
                     /locus_tag="PKL93_03425"
     CDS             69117..>69362
                     /locus_tag="PKL93_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ70345.1"
                     /translation="MGIVYQKNKKTGITYVYKNEAYWDKEKQQSRAKRTLIGKLDPNT
                     GEILPTRSYRKDTEKKDSISKKPGSVPITKIRRDFYGA"
     assembly_gap    69363..69462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <69463..>70827
                     /locus_tag="PKL93_03430"
     CDS             <69463..>70827
                     /locus_tag="PKL93_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNQ70346.1"
                     /translation="KKPGSVPITKIRRDFYGACYLLDQVGKITEVEADLKACFPDRYR
                     MILSIAYYLILEENNSLSRFSHWQRLHVHPYGEDIPSQRSSELFQSISEEERMMFFKK
                     QGRRRIEKEYWAFDITSISSYSETLRQVKKGRNKEYERLPQINLALLFGEQSGLPFYY
                     RKLPGNITDVKTVKQLMAEFNVMGYKKVSVLLDRGFYSRENINLLFKNHQKFIIGVKL
                     NLNYVKETLEEERENLQLWSNLQPQFGVYGLCRTIQWEYEQERPNKGDVLSSKRRAYL
                     HLFYNGEKAAKDQAEMNDYLTRLYNDLVNNTREESRMKDYDRFFTVTSTPKRGRKVTP
                     KEEAMREAARNYGYFALLSNEVNDPFEALSLYRSKDIVEKGFGNLKDRLNFRRMQVSS
                     ELSLNGKLFVEFVALIYLSYIKKKMQDTGLFDNWTLQGLLDELDTIERFEYPEHGRLI
                     GEVT"
BASE COUNT        19404 a        16086 c        16313 g        18924 t
ORIGIN      
        1 tagttaaacc ctagtgtcct tatattattc tccaataatc aagggtcaga ccgggtatag
       61 agagaaaacg gtcgatggca gtttgtactg atcatccttc ttagaaattg agtatttctt
      121 tgaaagatca tatggagcca ggcaggctgc acttaactgg ttcgttgctc tcaaccaaac
      181 ggggtgtgta cgtgttacct cccccgtctg ccagccccaa ttgtctcctt cagcattgtc
      241 gcccttgttt gtaagtataa gcctgcaact gtatcttccg gaggtctccc tgtttgttcc
      301 gtcgactact actgcgtcga atggggaata gctccactca ggaatgaaga gtatccacgt
      361 cttttcatag tagttgtgcc cttctatctt catgtacttg ttcaaaggaa aactcaaaac
      421 acccacgtaa tcgtggtctg caatgttatg gctcctcccg taggttatct gccgctcagc
      481 tccgtttatg cgcatcgaag gcatatatgc agatattgcc agtgattgga cttgagcact
      541 ttcagaatta atactcagcg aatagtttga ttcgtcaaga tattcaggag caattagctg
      601 atcgttgaca atgttcttgt ggacatcctc aaacaacgtg tgagtatatg aggttcccgg
      661 aacattgctg tttgtgctcc tcgatattga gtaccttcca tcctcgagat tctcgaaaac
      721 gtagcgtctt acaattactc cgggttcctt tgatttttct gcgtcctggt aggtgaaaga
      781 gaacttgtgg agagttccca gcggactagc ggcttctatt gcctcgtcaa cccccatgag
      841 ctgaatctgc tgattatcga gagttaactt cacgccgcca gtagaatcat tgaacataga
      901 ataaaccttg tccaagaaac cgaatgcgag cttcatcccc tggggaattt caccgtccac
      961 agagacgtcc atggtctccc cactattcca aaaaagacgc acgacaatgc cgctgtcttt
     1021 cccgagtgct ttcaaagctg catcatctgc ctgattgtag aaccatatat catcagcatt
     1081 gtacgcgggt tgtatcaaaa ggctatcccc accgatggca taagtaacaa atacctctag
     1141 ccacttccag aattcctcta cttctgccgc taaataggaa ttgtagttct cgaagtgaag
     1201 ttctgctaga ttactgcccc tgttgtgctc gtactctgta agcaggttga gagcgtacag
     1261 ctggcgagtt gtcattgcct tgaatagatt tatgtatgta tttacatagt cagtgaagat
     1321 ctccgagtcg gacgaacaac gtttgtagaa tccgaggcct cctttcgcag cctcgtcgat
     1381 atgctttccg cttcttagaa gattcagagc gttggtcaca tacaactcga aaatcgattt
     1441 gtaactcgtt tcagcagttc caccaacaat tactttcttg atgttaagca ttacctgatc
     1501 aagcttgctt tcgtttatct cttccataag ttcttgaatg cttatatctg tggagtgagt
     1561 tgcttcaagt gtggttatgt agagatattt gttttggcag gcttctacag gtgtgaagta
     1621 gtcctgatcg atcgtcctta tgtaagtgtc atagtattgt tcgtcgagct tttcgagtac
     1681 ttcctgaatc tccgtgtcaa ttacttccaa cttactggag acgtcgtcaa gtttttcgtt
     1741 cacttctctg atttgatcaa gaatctcctt tatctcttcc tcgttaccat ctccaagacc
     1801 gaaaaggctt ttcagaatcc aggaaaaggc cttgtctttg gccttatcat agatcgtacc
     1861 tccgatctcc tttagaagtt ctttcagaat atcggatatt cccagtccga gagtcggttc
     1921 cacaccaaaa tcgaaggctg ttccttcatc aatggcttct actaggctaa ttacgaaagc
     1981 cttatagttg cctccaaatt gctcttccac gaatgaaaca agtctcttca ttgaaaagta
     2041 tgaagtctcg aacgaaggat ggatctgact gagaagatta actccatcgg gaatattgaa
     2101 tgctccccga atttcctgca aggcctctgc gtaactgata cctcttttga acatgtaggc
     2161 agtgaaaaga gaagtcaagg gatttacggg aatcagctca gtcctgaatg attcttctac
     2221 gactgacgcg agcaaatcct tatcttcgtt atcatttgag gaatcgaatg aacagagaat
     2281 caccaacggg aactctgtac caagaggaat ctcaacagag aatctgccgt aattgtctgt
     2341 gtgatgtggt tcttcaagca actggtttcc atcgacatcc aggagtcgca cttctgctcc
     2401 cgatagatac tcgtcagtaa aaacagtacc cttgataata cgagtattcg ggacttttat
     2461 gcaaccggca agaataatta agagcgacaa agagcaaata agcaacgctt ttctcacaat
     2521 atcgcctcca gtctagcgtt gatgatcagg aacaagaagc atagagaccg actttgaatt
     2581 tggtcgtagg agtttgaatg cagtttggac gaagtattat atcagctgtg atttcgaaca
     2641 ataacgaatt tacagagtcc tttcgaaatc agcatgtgag tcaaatttaa cagaacatac
     2701 taaaacggga gtaaataagt agaaatttct taagagctta tatgggttca tcccccaact
     2761 aaattcattg cggtcccgac atgtgttcca agaaatcaag actagacatt aataatgcag
     2821 tatagacatc gactgaagaa gccgtgatag ttgcaacctg aactaccctc ttagaagctt
     2881 cgcgaaagat ttctgaaaca tgccaaacgg caactgattc tcaatttaca gtaactaatc
     2941 ttttttcttc aatgaattct agcgaacttc aagaactccc tcccatcgtt atcgtaatcc
     3001 ctgaacacga aggcctcaac gttttgatct ctgcctctca ggaattcaac accgaaatag
     3061 cagtttagat aaggtggtgc ataggtgaag aaaagatcct tgacggcggg ccggtattcg
     3121 aaattcatag catcgtggtg cgcatttgtg ttggaaaaga gcagacccct ttcggttgca
     3181 tagagatcgt agcctgtgtc aatatcacga caatagtatc ttccacagta ctcttggaga
     3241 tcacatggtg aaagtctctt ctcgaatggt ctgaaccgaa ctgcaccgct gtgatttgtt
     3301 aatagaagcc ttttctctcc attctcccgg aagatttccc cttcaaactc acgatccatc
     3361 tcttcgaatc ggagtcctct taggggcgat agtatgtgta ccccttcacc gttcatatct
     3421 agcttgagga agccctcctc aacgaaaagc ctgcagattt gagcgctctt tctatcggca
     3481 aaccacttgt cgccttcctt catctcttca agccaatctt cagaaagttc ttcaggtttt
     3541 gtgccttcgt ctttgctgtc aagacacgtc gaatcttctc caagaaccca gtcggcaatt
     3601 gcttcgtaga tctcgatgcc gagtttcacc agcatcctgt gaaggaagtc gttacgattc
     3661 gccagaacaa caactgaaag ggattcggca ggtatcctgt aaatggcagc gttaaatcct
     3721 aggcttcctc cctcgtgcat tacagtcgtc ttaccctttc tcttcgtcaa gaataggccg
     3781 taaccgtagt cgcaccggtt gccgttgttc agttttaccg gagaaatgat cttctccgac
     3841 aacctctccg gatcttcacc aagcatcttc ttgtggaaag ccttttccca aatgagaaga
     3901 tcatcaagcg tactccacaa ttgaccggct cctgtacact ccattagatc gagagctttg
     3961 gatagcctta ccgatttgtc taaaggtaat ctgaaagact tggaatgcag gtagtcgtat
     4021 tcgcagtagc catcaacaac attctttatc agtctagtat tgtcttctct gaaaaaagtg
     4081 ttcgtcattc ctaatgggga gaagatctct cttgttgcga attcattcaa tgtcgatcca
     4141 gtgattttct caaccaatct accaagaagg aagtaacccg aattgccgta tgcccacctt
     4201 ttgccaggct cgaactcgag ccatcgaagg tttcttagaa ggttgtacgc ttctgtcgaa
     4261 gtcagatagt cactctctct taagcccatt atgtattgcg ataccttgta taaatccggg
     4321 aggccatttg tcatgtgtat cagatcgaag actcttatct cacttgcatg gcttcccaga
     4381 tctgggaaga aatcgcttat cctccgagac acatcgagat gcttttcttc gagcaaagca
     4441 atgcacatcg ccgtgaactg cttgctcacc gaaccgatgt gaaagacact gtcagtcttt
     4501 acaggaattc cctgttcttc atttgctagc ccaaaagtct cttttaggac cacttctctt
     4561 tctttgaaga cacccacggc aactccagtg gttccaaggt cttcgaacaa acaactgata
     4621 tattttccag ccataagtta tccctccttc tcaagagttg catgctaact tgagcctgct
     4681 atcgattgag atattatact ttagatcata gaattcccaa tcttcctggg caggatgagg
     4741 cattcaagag gttctctccg gtagagacag gtttctttcc cgtcacagat cttttcgcag
     4801 aagatgcaag tgccacgggc tttgccaatc taagtgtcgg ccacgccggt gggggaacag
     4861 tttcggcaga ggggatcttc tcaaatcatg aaggcgttca aagaaggagc gataatcggc
     4921 agcttctcta agatacacga ttgtacaggt tatttctaaa tggttttcat gggcattaat
     4981 tatggataaa gtctcatgag tctggagacg atcccacttt ttccacccaa gttcaattcc
     5041 actggaaaca tttgattcaa aggaccttcc gcgtttcgga atcccacctt ccgtttgcat
     5101 attattgaga acttgaatcg tttcaggttc cagttagagg gaggaagatt tctcccaaag
     5161 acggtgaaca ccgtctaaaa attcgataac cgttattgaa tcgaatattt ccggcattgt
     5221 atgtttcaca gcacaggttc tcccaaagaa gttcttcgcc gttttacaca aattcctaca
     5281 atgattacca cagtagccac ccgacgactt aatcagagag accaaagagt agcttcttgt
     5341 tttttccgga attcccgtca tagctagccg caagtgtgct acactcttcg cagtcgggaa
     5401 acctagactg attttgtttg attacgactt ccccgttacc ggaggcattt atgaagttga
     5461 catcgatctt catcgtggtt gcatcgctgt tctactttat gatggggttt gctacccaat
     5521 ccattaacag tgccttaccc gcaatcgcgg tggaactcga gatgaaccac agcatgacag
     5581 gtcttgttct tggtctccca tcccttctct atcaggtagc cgttattgtt gccgcagctc
     5641 tctcaagaaa gtctgggcca tttctttccg cttcgctcgg catctcctgt ataactttct
     5701 cagtgatctt tatggcgctt tcacggacct actatacttt tctgatagga aggctgttgt
     5761 ttggtttcgg actgggtgca acggagatat ccattgcgct cggcgtttcg cagttagctt
     5821 tcaagcgaac tgggggtgta ctgaattcag tctactcttt ctttgctttg gggagcatcg
     5881 tggctccttt ggtagtttct gctgtcttga ccgaaacaaa catgtggaat gttcccatcc
     5941 ttattgctgc agcgctgttt cttgtagtgg tttttctatc atggaccgta tggaagaaag
     6001 ctgcgaaaga gtctctttca gatgtcgaga agcgaaggat cactttgccg agagattcga
     6061 tgttctggct cgtggctgct ggagggcttc tgtatgttgg ttatgaggtg ggtctcttct
     6121 catggctctc gagtttcgct tacgaggaga agctcctgcc gttaagaatg gccagtctca
     6181 caacatctat tcttgcgctt ggtctgttcg tcggcagaat agtgactgga gttgttgtcg
     6241 acaaatttgg actccagaga actctcgtag ttctcggaac gatatctctg gcgggatttt
     6301 ctatagcttt ctttgcgggg aattctattc ttctttcact tggagtcttc ataacaggtc
     6361 tggggttttc gggaacattt ccaacgcttc aggcgataat cgtatccggc tacaggcaga
     6421 accgcgaaac ggtcttggcg atttttgcag cagcgggctc tgtcggggct gttacgacga
     6481 atattcttgt tggaggagta agtgacagaa caaacctcgc ttatggaatg tcagttatcg
     6541 gggttcagat agcggccgcc cttctcatct tcacttctat ataccttatt cagcgaaaga
     6601 gaaagaagct tgcaacttca tgaatatagc aagtaagact gcttctttca gaacgagggt
     6661 agatcgagtc gtatgaactt caagagctgg actcatttct caagcgcgga tttacaatga
     6721 ttcaccaaaa gagcagcatc acaacatccc tttcgaagaa aagagaactc actcttgtat
     6781 tattatcata tttagaaaga ctcatactaa gccaaccact tcactccggg aggtaattat
     6841 tatgggcatc ggtataatta ttcgatgcaa ggaatgcgac tattcgaaga atctccagat
     6901 tggtgtcgga atgatgtacg ctccacacaa catcttgaat cttgaatcga agtcgcccat
     6961 cttgaaatca ctcattcctt cgaaaaagac catcgagaag ataaagatgc ttatagaaag
     7021 taagaacgca gttcttgccg atgactacgg tcattacatc tacagatgtc caggatgtag
     7081 cgagctattc gacagattct tcattcacct tgactatgac gatgaaagtt ttgaacccag
     7141 ttaccgttgc ggtaagtgca gatcaacgct cgagagaatc gatcataact ccgatgaagg
     7201 ctcaatcgaa gagagaatcg gcaagatcct ggcatccttt ccctgcccga agtgtgggaa
     7261 ccgttccctc tacgtcgatt ctgactgcac actcatgtgg gattgatttt ttcttaaaac
     7321 cgatctcttc tatctgctat actgtttgta gatgccagtc ttcggttgcc cgcattggca
     7381 gctgttctaa acctatacaa agcattggaa gcgaggagag ataaactgtg aaagagaaag
     7441 atcgcaaaga tctgtctctc taaagacaag gaaagctgat cgagtcgttc agattgcgat
     7501 tcgaaagtaa tttgagtcgt cacagggcaa tcgactgggt ccttgtccgg gaaagactga
     7561 aggtaaatcc cgagaagctc cggtcgtcga acgagatcga gattaccgga ggagaacccg
     7621 acgtcgttgg gttgacaagg atacgggaga gttcgtcttc tatgactgct cacccgagag
     7681 ccccacaggg cgaagaagcc tttgctacga tcgaccggcc ctgaagtcga tgaaaaggga
     7741 caagccgtcc gatagtgcca tctatatggc cgagaaaatg ggcatcgaga ttctgacgga
     7801 agctcagtat agggagctgc agagcttcgg gagctttgat acaaagacat ccgcctgggt
     7861 gaagactccg gagggcataa gaaaactggg cgctcttttc tgtgaccgaa gatgtggcac
     7921 cgtcttcgtc tatcatggtg gcgccattca tactacagtg tcagaaactt caggggttca
     7981 ttgaaggtat agaaaagacg gtcatttcat tctgcggtga gaatcctccg gattcttcaa
     8041 gcttcgaagc aaaagaaaat gctacgcaaa gcatcataat tgaatctact cgaagtcgag
     8101 atgaaggaga ccgagaacgc ttccctttga gttagatttg gatttgatta tctgcatgtc
     8161 aaccagtggt tcttgagaag gacgtaacgc atcataattg aatctattcg agcgggagct
     8221 gatacatgga aacaaatcta ctcgaagtcg agatgaagga gaccaagagc gcttcccttt
     8281 gagttagatt tggatttgat tatctgcatg tcaaccagtg gttcttgaga aggatggtag
     8341 gcgatgaaga aataagctat aatcaactcg aaacttatac ttgcatttag tacatctgga
     8401 aagtctcctt tctttgtttt ttctgttcga aacgtttgag tatttgctcc ataaaatgac
     8461 aggattcgaa gctccctcga aagagtagtt tactgaagaa gcgaagtgag agaagatttt
     8521 tctctacctt gtcagaaagg aagcatatgt tctctttttt actcgtcatt atttacgtgg
     8581 cctttgtcag tctgggctta cgggattctc tgctgggctc tgcctggcca gtgatgggga
     8641 aagcgttcgc agttcctcta tcttttgcag gcattgtttc aatgatcatt gccggccgag
     8701 cgatcctttc aagcctcctg tcagatcggt tgaatcgaaa acttggcacg ggcctcgtaa
     8761 cgtcgctcag cgtgggtatg acggcagttg ctctcattgg attctcggtt gcgaattcat
     8821 ttgcaactat ctgtttgtgg gctataccct tcgtcctcgg tgccggtgcc gtcgatgccg
     8881 cactgaacaa ttacgttgcc ttgcactttg cctcctgaca tatgagccgg cttcactgtt
     8941 tctggggagt tggggctttc ataagccctt atataatgag ttattttctt ggaacacaac
     9001 tgagatagca tagaggacac ggagcggtgt ctgttatcca gattgtgttg accttggttc
     9061 tcttcagtgg cctttgcctc tggagaagtg gtaagagtga aagagatgga gaaagtaaaa
     9121 cctcttcgaa gtttttgagc gttggcaaag cgcttaggat aagaggtgtt ctgtttgttc
     9181 tcattgcatt tttcgtatac tgcagaccag aaacaactgc tggactatgg gcgagcagtt
     9241 atctcgtcga gtaccaagga gtggatgttg agaccgaagc gatcttttca tcgctcttca
     9301 gtccgggaat tactgttggc aggttcttga atggtttcat cgtcgacagg ttcggggata
     9361 aaagagtgat tcgctatggg atctcaacat tgactgtggg agctattctt gtgggcattc
     9421 ccttcaagtc cgagatcttc gccttggggg gcttgattgt aataggactg ggttgcgcca
     9481 atctgtcagt aattcacgca actccatcga attttggaag ggagaactcg caagcaatta
     9541 ttggaattca gaaagtcagc acttatgtcg gcacaacttt catgccccct ctgtttggcc
     9601 tgattgcaaa cggcgtgaac attgggctgt atcccgtata tcttgcggtt cttgcagtct
     9661 tgatgccgac cataaccgaa acccttaacc tatccattgg gtagtaatga atggcttacg
     9721 aggtgtacaa tgcttggagc gattaccgga gacgtagttg gatcgagatt tgaattcaat
     9781 aaccaccgtg acaagaattt cgaactcttc actgaagatt gctttgcaac agatgacagc
     9841 ataatgaccc tagccgtcgc aaaggcaata atggagaccg aaaaaagtat aaacaaatcg
     9901 gggataaaga gcagggcaga cagcgattat ctctcgctgc tgcaaatgct ggccataaaa
     9961 tacatgcagg aaataggcag agactacccg aactgcggtt acggtggaat gtttggcaga
    10021 tgggtcttta gcgattgtcc agaaccatat aacagttttg gaaacggagc cgccatgcgg
    10081 atcagtccag tcggtttcgt tgcagagtca gaggaagagg ctgtcagcct ctccggtgcg
    10141 gtaacttctg tcactcacaa tcacagggaa gggatcaaag gtgccgaagc tacagtcatg
    10201 gccatcttcc ttgcgcgcca tggcctttcg aagagagaaa ttcgaagcag gatatccgag
    10261 gactactatc cccttaactt caagatcgat gaaataaggg acacatatga attcagtgag
    10321 acctgccagg aaaccgtccc tcaggctatc gaatgttttc ttgaatcagt ctccttcgaa
    10381 gatgcagtcc ggattgctgt atctctgggc ggagacagcg acaccattgc ggcaataacc
    10441 ggggccatag cccaggctta ctatggcgtt cctgactgga tacgcctcaa aactctttct
    10501 tacctggaca ggagacttcg cttcatctac gacgaatggg aggcctttct cgatagattg
    10561 ggcatagtct tttagttctt caggcagaca taaagccttt ctatgtgata atttcaccta
    10621 actcttgccc tgcctcgagg ctttccccct cacacaagag ttacagcgag taacggtatt
    10681 tgccacttca tgaagaatct tccaaagctt cgtcaatgag ttttttgtat atatgtatta
    10741 tcataataaa aaccacgata ccggggattt aaggtcgata aacggcttct cctccttctt
    10801 aagaactcta tttgactttg gaaccgtctg tgaaaaactc tataatcata ttaatagtca
    10861 caaacctgta aaggaggggt gtttatgaaa agagttctag taattctcat tgttgttttc
    10921 ctagtgacat cgactgtgat gttcgcagcg gatatcaaga gagggggtac gctcaggatt
    10981 acctcgatga aacaggggat tctgatagag aacttcaatc ccttctcgcc gaattccaat
    11041 gaaatggcga ttggaggttt ctacgaaact ctcatttact tcaacccgat gacgggaaag
    11101 atcatgaact ggctagcaga gagctatgag tggtctgacg atctcctgga gttgaccttc
    11161 aatctgagag aaggtgttga atggaatgac ggaacacctt ttaccgagaa ggatgtacta
    11221 ttcaccgtaa accttggaaa gaacaacaag gcactcgacg tcgcgagcct gtggtcgaca
    11281 ggcgttgttg gagtccgatc agacaaaccg atgaccgtta ctttcacctt ttccgatgtc
    11341 aacacgacca tcattcagag attctccagc gtcttcattg tccccgaaca catctggtcg
    11401 aaagtcgatg accctctgac ctggataggt gagggaattc ctgtgggcac cggaccattc
    11461 gttctcaagg aaggctcgtt cagcgagcag tccttcagcg tggtaagaaa tccgaattac
    11521 tggcagatag gggaagacgg aaagcctctt ccctatatcg acgaagtctt gacactcaca
    11581 actacaaacg agcaggtccc tctcagactt gccagaggcg agttcgactg ggcaggctac
    11641 ttcgttgcga acatagacga ggtctttgta aaggccgatc cagagcactt caagtactgg
    11701 ctgcctgagg gaaacctggt atttctaaac ctcaacaacc tcaagtggcc cttcgacaac
    11761 tacaacatga gagcggcgat tgcggccgcg atcgatccag tcgagataac cagaataatg
    11821 gctagcggcg ccgttcccgc ttctctggcc ggcgtcaagg cgagttatct aaggcttgcc
    11881 gagaagggtt taaacgaata cggaattgaa tacgaccccg attatgcaag ataccttatc
    11941 gagaaggaag gttataagct gaacaggaac ggaatctacg agaaggacgg taagtcgcta
    12001 tcgttgaatc tgtacgtgcc aacgggatgg accgactgga taatgggtgc cgaggaagta
    12061 tccaggcagc tgaaggagat cggaatagag gccatcgtca ctttggcagc gtggccttct
    12121 ccctacaaag acatgatgta caacggcaac tacgagatgc tgttcggcat agccgtgaca
    12181 ggaaccagcc catactacca gttcgacaac tgggttcatt ccaaacactg ggcgccgatc
    12241 ggcgaaaatg ccgggaacta ctacgggatg agatataaga acgagagaat cgacgaactc
    12301 ctggatagct acaagaggct taccgatccc gaagcccagg acgccgtaat ggaagaggta
    12361 ttgacaatct tcttgagaga tcttccatcc gttcccatgt tctttaatcc cgtatggttc
    12421 gagtacagta caaggaactt tgtcggctgg cccaacgccg aaaatccgta cgcggaaccg
    12481 agaccgaccg gaatggacaa gatgcccatt ctgctttcta tacatctgcg ttaatcaact
    12541 gatatttccc gcaggcttaa ctgcctgcgg gttttgttaa aaaccacgag agggggtaag
    12601 aaaggctttc tttctgcatg gaaagagctc tttctgaaca gcattggctt acttcgctag
    12661 aaagatcggt ttactgctat tcgctttgtt cgtggcagtc actctgaatt tcttcatacc
    12721 gagaatgatg cccggtgatc ccgcacagga tttgatggac aagatgcagg gcattcccct
    12781 tgaatcgctc gaagctataa aggccgcctt cggtgtagat tcgaccgacc ctctcctgct
    12841 ccagtacgga aagtacctgc tgaaccttat taaaggtgat cttggaatct ctatttcaag
    12901 attcccgaca cctgtcgggc gggttctcca ggttgccgtt ccctggaccg ttggtctcat
    12961 gggtacggca acgattatca gcttttttct tggcacgata attggaatag gcgttgcatg
    13021 gaaacgcaat acgaagttgg cttctttcac cgtggggctc tttattttcg tacgttcctt
    13081 tccatatttc tggcttggtt tattgctgat ctactttcta gctttcagaa ttcccatctt
    13141 tcctctcgga aatgcctata ctccgcaact ggaaagaggc actcttgagt ttttcctttc
    13201 ggtcgtcaag catgcgattc ttccagcatt gaccatagtc atctcttcga tgggagcatg
    13261 gattctgacc atgagaaaca atatgataaa cgttcttgcg gaggactaca taacagtcgc
    13321 cgaggcaaag ggacttcctc tgagaagaat caagactctg tacgcagcga aaaacgctat
    13381 actcccttcg atcacgggct ttgcaatgtc tcttggcttc gttgtaggag ggggcctact
    13441 cactgaaatg gtcttttctt acccgggagt tggtctgatg ttgtatcagg ccgttcaaag
    13501 caaagactat ccccttatgc aggccatttt tctcttcatt tcggtggctg ttttagttgc
    13561 gaacttcatt gcagatatcg cggtcctcat actagacccg cgcgtcaggg acggtggcaa
    13621 gtgatgttca gagatgtctt ttcgcttttc atcgaaaatc gtaaggcact gatcggtctg
    13681 ataatcctgg ctttcttctc tatcgtagcg atatttgcac cgctgatcgc gccttacgat
    13741 ccaaagacaa acaaggttga agttctgagc atagtcgaag aggaaattgg aagaacaaag
    13801 acggtcgaga gggaggagat cgacgaattt accgaaaaaa gagtatacac gcagtctata
    13861 acaactacct acgaaaaagt caccacaaaa cttccgctta acgccaaacc ttcccgaaac
    13921 cacttgctgg gaacgacacg ggcaggacag gacctcttct cccaaatggt ctatggaaca
    13981 agaatcacac ttgcagtcgg gctcattacc ggacttttca caacgctcct agcccttacc
    14041 ctggctctcg tggcagggta tttcggcggc atagtggatg atataatctc ccttttcacg
    14101 aacgtattcc ttgttatccc gagcctgcct ttgatgatag tgatcgccgc ttacataacc
    14161 gtgaagggag tcatccccat agtcctgatt ctggggctca ccagctggcc ttggcctacg
    14221 aggatacttc gttcccaggt tgtgagcctc aagaaccgtg atttcgtgag agtatccagg
    14281 attcttggcg aaagacccct gtacatagtc ttcagggaaa tactcccgaa catgatctcg
    14341 ctagttatgg cctctttctt cacatccact atggcggcaa tcatgggtga agcgactcta
    14401 gagttccttg ggttgggcaa cgtatcggtg gtaagctggg gaacgattct gtactgggct
    14461 cagaacagcg gggcgctctt atccggcgcg tggtggtggt tcctccctcc cggtatctgc
    14521 atagccttga tcggtacttc cttcgccctt atgaacttcg ccatagacga gatctccaat
    14581 ccgaaattga ggaagagatg atttgatgaa tagacaagag agcgaacagc tactccagtg
    14641 cagaaatctt gtggccggct acaggatcaa aagcggtttt gttcatgccg ttgacgatgt
    14701 ctctttcgat ctcgagcggg gaggctttct cggtatcgcg ggtgagtctg ggtgtggaaa
    14761 gtctactctt gccttcgcaa ttatgaggct tctgaaggac aatgccacga ttgaaaacgg
    14821 cagtttgttc ttcaatggaa ccgacctcgt tggacttagg gaagaggaga tgaagaagat
    14881 ccgctgggtc gatatctcga tggcctttca gtcggcgatg aatgccctaa accccgttct
    14941 ttcaatcgga gagcagctca cagatgtcat ccatgcccac ggtgaagtta gacagagcga
    15001 agcgcgtgat agagcaatag aggctctaaa gctcgtagat attcctcgtg acaggttcaa
    15061 ttcctatcct catcagcttt cgggaggaat gaagcaaagg gtgatgatag cgatggcctt
    15121 gatcctcaat cccgacctga taataatgga cgaacccaca acggcactgg atgtagttgt
    15181 acaaaggacc ataatcgaaa agatacagga gctgagaaag tccctgggat tctctgtgat
    15241 attcataacg catgatctct ctttgcttgt ggaaatcagc gacactctcg caataatgta
    15301 cgcaggcaag atagtggagt acggcaactc cgatgagttg ttcaacatgc cgctccaccc
    15361 ctacacaata ggactcatga actcctttcc atccctcact ggcaagatta ccagaatggg
    15421 cgggatagag ggaaaaccgc ccgaccttct aaatccgcct gaaggttgca ggttccatcc
    15481 acgctgccct catgcaatgg acatttgcga aaagtcctgc ccggctcttc aaaaagtgga
    15541 ccctcggcac agtgtagcgt gtttcctgca caaaggagct gaaaacctgt gagcggcgac
    15601 attctggtcc tcgagggagt aagaaaacac tttcccgtga actcactgag gagacacggc
    15661 aaatttgttc acgcaatgga cgacatatcg ttctctctga gaagcggcga agtcctggcg
    15721 attgtcggag agtccggcag tggaaagacc acgacggcta acgtgatatc acgtatatac
    15781 agacaaacag aggggagcgt agtcttcgaa ggcagagagc tgagcggcaa aatgactcgc
    15841 acagaagaac taaagtatca caagagagtt cagatgatct ttcaggatcc attcggggcg
    15901 ctgaacccga cgcgcacaat aggtagaatc atggaacggc ccttcgttat tcacaggatt
    15961 gccgaggaca ggaaggccgt cagtgaaaag gtcgacagca cattgacaca ggtaggcctt
    16021 gaaccccccg atcagttcaa gaggaagttc ccccacgagc tctccggtgg acagaggcag
    16081 agggtcaaca tagcccgtgc ctttgccgtc gaaccggacc ttatactggc cgatgagcca
    16141 acctcaatgc tggatgtgtc aaacaggatg agcataatga atatgatgct aagtctcaaa
    16201 gaaagccatg gagtttcatt catatacatc actcacgatt tggcaggcgc cagatacatg
    16261 agcgacagga taatcgtcat gtatgcggga atgatcatgg aaatcggtcc tgcagaagag
    16321 ataattacga actctttcca cccttacaca aagcttctta aatcggcggc accggcccca
    16381 gaaacgggtc taaaacgaaa accgctcatt acgggaggag atataccctc tctgatcgat
    16441 ccgccgaacg gctgtcgctt ccacccacga tgccccttcg ccaaagaggt ttgttcactc
    16501 gagatacccc ctatgaagaa gatcggagat gaccactacg ctcgatgcgt tctttaactt
    16561 catagacgcc tgatcgggat ttgtagccac atttcaacca atccttttcg agaaaaacgg
    16621 tgttgcaaat ccgaaaagcc tttatatgaa gttgtggttc tgctctcgtg cttgttcgca
    16681 ccttagtttt gtggaggcgc tctctgaaat gaaaaagaat gccggagttc tgtacgatcg
    16741 agatgttgtt tccgcctgtg tgcagataac tgaatcggga ttcactttcg aatcgaactg
    16801 agttgacctt ccgaaggcgg agcagcttca agccactgtc caaaacgaga cttgccgttc
    16861 tgcatctagc aggcccttac cagcagaatc acaattaagc tttctaggtg agtccttgtt
    16921 cttgagttgt atcagaggag agtctgtcgc cctcgtgata tgtcactgca tcaagacggt
    16981 gcaatccgta agtggatcgg gattctcttg attagggact ttcttccgaa aaatcagatc
    17041 accatctgta cgatctggtg aatctacgca tcatcattag ttcaagaata caggcttttc
    17101 atttcgccta agcgttcttt ccgtccatca tttgaataaa caacctggcg atattcgggt
    17161 cgaattgctt tctggcgtct tctcttatca cctttaacgc agcttccctc ttttcttcta
    17221 acgtgccctc caccctgttc attacgcgat cgtaggctcc cgctatggat attatccgtg
    17281 atatcagagg tatttgctca cccttaagac ctctcggata gccgcttcca tcccaccttt
    17341 cgtgatggct gtaagtgtac tcggcaatgt ctaaggtttc gtcgaagaga ttcaaaattc
    17401 gataaccaac aatggagtgt tgctttatct tctctctttc ttcctctgtc aaagccgtgt
    17461 aagtatcctt agaaaggatg ctttcgtcga gtacgatctt acctatgtca tgcaaatatc
    17521 cggctctttt caggacgttt atctgcgact gagagagctt catggcagag ccgatctccc
    17581 cacacaattc gcttacggca atagagtgct gtttttccat aggatacttt gtgtgaagag
    17641 tatcgataat cgagtcaatt aactgcctgt tcaaagattt tcggtgtatc gtcttgtctt
    17701 tgtacatgaa gctctcagcg ttggccatta tttcattcag tgactgatcg gctcgggtct
    17761 tagtatcaaa gccaagtgaa atgctgcatt taactgcttg gacacgggca tcggagaagc
    17821 cttctttgat tcttgaagca atactttcag tttccttttc gctcgttccc ggaagaataa
    17881 cgacaaactc atcgccaccc acacgtgcta ttagatctct ctctctgcac gattctaaaa
    17941 ggattctggc tgactctttt attaactcgt ctcccgccgc atggccgaag acatcatttg
    18001 tcatcttcaa gccgttaatg tctgcaacga ttacggatat gggaaggttc tcttcaacat
    18061 cgactgcttt ctgaatctga gccaaactgc ttctgttgag aaggccggtt agaacatcgt
    18121 gagagctaag gtatttgatt tcctcctctc tctttttgcg ctcggtgatg tccgaaaaag
    18181 tgattacacc gccaacgact tctccgtttc tcatttgagg ataggaacga tactcgacat
    18241 cgaaagacga gccgtcggcc ttccagaaga cctcgtcatc cgtctggaac cctctcccat
    18301 gtttgataga cctgacaatc ttacattctt caatcgggaa cgtcgttcca tctcgccgac
    18361 tgtggtgaat caaccgatga acgttctttc cgagaatatc ttctggacca ctgaatccca
    18421 acagctttac ggagctcgca ttgcagaatg tgcagtcacc attcagatct ataccatata
    18481 tcccttctgc cgtggagtca agcagaagct gtagctcatt cttgctttct ttcaattcct
    18541 catttgccct ctgcagttgc ctggttcttt ctatcacact ctcttcaagg ttattgatca
    18601 agaactgtag tttgtctgca actctgttca ggctttcaga aatcatcccg atttcatcac
    18661 ttcttacaac ctctactcgt ttagacagat cacctttaga caaggcagtt gaaacctcaa
    18721 tcagattgtc tagaggcttc atcaattttc tcgctaaaat atggtatatg attagtaaga
    18781 gcactaaggc aaccacaacg ataatcacgg tcagatggat actgtctatc acgtccttca
    18841 tatagaaggc ctcgggaatt gcggacatca caacccatgt agcattgagt atttcgatct
    18901 cccgcatgtt aatgaacagc ttttcctctt cacctctgta gaataaaggt gattcccggt
    18961 ttgtagtgga ctgaatgaaa gccttctcaa tatcgggaaa gccgatatcg tcaacggttc
    19021 gctggctgaa agagccatct ggaaggactg tgaagttatc aacacccata gagttcgcta
    19081 ttagagagcc cgtttccttg tcggcaacta ttgcgtagcc tgagtagtct cgaacagcat
    19141 ctacaaggaa actaccaatg tcagaaagaa ggatatgtgt ccccattacg ccttgaagac
    19201 ttccactggc atcataaact ggccacgaga aggagactgt aaggtcatcc atcacgaagt
    19261 gcttgtataa aggagataac gccggccctc cagattcctc ggcggcctta taccaggcgc
    19321 gtgtacgtgg gtcgaactta ccggcttgga cgaccctctc ccctgcggtc agatcctcat
    19381 taaccgagta gtaccatgag ttgcctcccg tagaggcgtc atttcgcatt atttcaatga
    19441 ctcccttttc atttctgagg gctccgtaat actccccgtt tgccgtaccg taactaaaac
    19501 tatatatatg agggctttga gcgctcaaca ctcccacaaa gaacttctct ctgagctttt
    19561 catttgacag atcgagaatt ccatattcga taaccttgtg gttgacttcg ttcacactgt
    19621 gaggtatatg cataaacgag gagacttgat tgtagatgtt ttcccccagg ttttcgacta
    19681 ttctttcagc ctctttttca gccgaggata gccatctcga aatgatcagg tatcctataa
    19741 cgattgtcga aaagagcagt attgaaacag acacgatagt aatgatactc tttattgaaa
    19801 tgctcttctt tctcaggctc atatgcctca ccttgataat tgtaattgtc gactaagtat
    19861 aaatgataac cgaaatcatt taagctactt ttaacagaat ccactagtca ttcctacaac
    19921 ctaagctaac ccaggagtat tcacttcatt atacaagtat cacaaaaggc caaaacctga
    19981 actctacgag ttgcacacag gatgttctct cttgaacaaa cacgacattc ttacgattca
    20041 aactcatgac gaaataattg ccagtgatta ctgtgccttc aatagtagtc gcttctatac
    20101 attagacaac acatccgaag acacttaaaa ataattatta catataattg caatgcttcg
    20161 tcatattacg catcaaattg agtcatgcaa cttcatgcca tagcaatatc gcagctcgta
    20221 attgaatcat ccaaagggtg aaacaagatc cttaacctcg gattctgcat gatgctagct
    20281 ggagtatagt atcatctctc ttcaacaaaa ccgggattgt gagtcgagaa ctgttaccac
    20341 tgtaattcga tccgggtcaa acatcacagc tgttccacgt tgtgaaatcg aaggggatgg
    20401 gatattcaat actgagcaaa ctcgtatttc cgtcgaagga attatctctt gctatagctt
    20461 tcgagaaatt cgatagctat cccttcattc taaggcctag cgatgagata gccctgtatc
    20521 ttatcgcagc cgcaactcag aagaaactgc ctctgctttt catttcccgc gccttcggcc
    20581 gaaacaaaat gtccaggctt gtgcgtcata gaaatcatgt cggttgttat ggtctcttcc
    20641 gcttctgcat tggtttgtcg acaaagagaa ctcatcggtc ttcggcatac tgagatcaag
    20701 aatcaccata tatctcgatg ctcatcgatc tgctgcatcg ttctattttc ttagacgctg
    20761 taaggacatc gtttcctttc aacattttct cgaggtctga agccgaatat tcagcaatgg
    20821 gatttttgca ggcacttcaa tcccccctaa caaatgaagc tttctccttc aatggagacg
    20881 cccaatctcc tccagcagtc ttctgagcaa aagggtgaat ttcttcttga gagccggtat
    20941 ctccttctcc tctccctttt caactgccag agtcttttga aacgctacaa atacttcata
    21001 aagctctttt gcgcccatac tgtcgaaagt gttctttacc ttgtaaacat tcccgaccgc
    21061 atccgagagc ctttcttctc tcatagctag atttacttca aaagaagaac atagattcca
    21121 ctcttgcatt actgaagagc attcttcttt cccttttgca tgaaatacct tcttccaaca
    21181 aaccaaaaca aaaaatggtc ccggagggac atgctccgag accaagttgg atgtaatagt
    21241 gaggtgttac gaggtgaatc gtgctttcag gattacgtga acctgaatac attgggcgct
    21301 caactatcta aatggtcgag ccccacacat ctattatgaa ccagactcac tcgataatcc
    21361 tagtcttttc tcaactccct ccaggaaact cccaggcggt ccaattataa acccgctgta
    21421 ctaatgatcc tgatcttaaa tccttcattg aggaaggagg gcgacgtagt ggcggggggt
    21481 gtcttttagg tcgaagaaca atgcataaga cgcaatgcac cggaaagcat cggctgtcgc
    21541 aatgccgcct tcagcagaag aggaagacca ttcgcgaggt gtttcaaaaa agcttcctgc
    21601 agttcgacgc aaatgccatc taatcgctga aaattaagaa agaagcttaa agaaacggtg
    21661 ccacgcgaag aaactacgtc gggcgttggt tgaaaagttg tcgccttttg gcgacaaaga
    21721 tcgtcagtta tcagatcttt tctagtgagt gaaagaaatt ccacgctctt ttcacgctat
    21781 ctggtttaca ttcgaaatgg aggtgatcat atgaagttac ttctgacttt aatgattgtc
    21841 gttaccggca ctctttgcct tgcgaaggga aattacacat tgcactcgga gattcagtac
    21901 aaatctcacg aggagagtct tctcaactgt ctagatatat acacacccga tgaattagaa
    21961 agcaagaccg gtgaaatccc ggtaatgata atgattcacg gtggtgcctg gagaaccgga
    22021 gataagtcta ataagaatgt cactgtgaat aaagttccat acttcatctc taacaactgg
    22081 atctttgtga gtataaacta cagactatca cctgaagtga aacacccggc acacgtcgaa
    22141 gatgtagccg ctgctattgc ctgggtcagg gaaaatatat cttcttatgg aggaaatgcc
    22201 gataaaatct tcatcatggg ccactctgca ggagcgcatc ttgcggctct cgtttctact
    22261 gatgagagct atctggcgag atatggaatc ggactagatg ctattaaggg agttgtacta
    22321 cttgacgggg ccggctacga tatttgcgcg agcatggaga agtatggtac agggatcatc
    22381 gcgacctcga tgtatctcaa cgcttttggt caggaagaag agtcatggta cgatgcttcg
    22441 ccaatcaatc atgttgctcc aggaaaatca attcctcctt tcctgatagt ttatgccggc
    22501 gaacgaataa agtcagaaga aatttccagt gacttcgctc taaaactccg gctttccgaa
    22561 gtgcctgttc gcttgtacca tgccaaagat aaaaatcacg ccgatgtgaa tatcgatctg
    22621 ggattagtcc aagatgatct gaccatcgta gtagcagact ttctgaacga tctactaaat
    22681 tcttccgagt gataagaacg attgtttgct gttagtaaaa gtacgaaaaa agagctttta
    22741 gagttcactt ctcttcagtc tcgtctctct tctcttctcg cgctcttctc gtagagaagc
    22801 tcttaacgct cttaccagaa cctgatccac acctcaaacg cgttagtgga gaaattctcc
    22861 gtaagatttt ttttgggact ccatttttca cgaatgagcg agaagattag agagagaaag
    22921 ggagagaaga gagagcttgg gagagagaaa gagagagaga ctgaagagat gagatcccgt
    22981 ataggagcat tacgggatga cactcctaca cgtcatcctg acaatgctcc tggtcaggat
    23041 ccggacgcgg tgcgaggtcc accgttgaat ggttctcggt tttccgaaaa gaacgtttgc
    23101 aagttccaga tgcaagttac aagtcgtaag agtagaacaa gcgtacagcg gctctcaaga
    23161 cctagatgct gaaacgggtt catgacggaa agagcagggt atcgagtgta gggtctgggg
    23221 tgtcgagaag agaagaactc accgtctagg cattttcaag taataatgct gatacagtta
    23281 tccagcttgc gccttcagga ggtcccacac attgtcatcc cgaacttgtt tcgggatctt
    23341 catgttgatc ttatccaaga acgaagaacc tggacgcgca gcgtcggaac gaagaactgc
    23401 tctttatagg ggcgggtcca cgatttgggg aaggacggtt tttcacgcgt acagcgggtc
    23461 ttcgttcttt agcgcacagc ggttcttgag aacgagatcc cgtacaggag cattacggga
    23521 tgacactcct aaacgtcatc ctgacaatgc tggtcaggat ctctaccttt cgcgaaaaat
    23581 ggaacagaca ataggagccg gtcagtctct attttcgctc tagacttctc gagagcgaag
    23641 caaaccctcg acaatgcatt ttttccgttt tctctttgga ggaaatagat gctgaacttg
    23701 attcagaatc tcatgttcat ggttcctgac attgccagaa cgaagaacag atacttgctc
    23761 cggaaagagc tctcctcgat gaacggtgaa ccattcaacg actaactaac ctttcttgaa
    23821 ctgtccaacc gtcagcctac ccacaatcta caactcataa ggctcttgtg gatttctagc
    23881 cgttttttaa tgcgtatgct tgaaaggttc tgatatcaga atagcaagca gaagtagtat
    23941 gaagataggg acaccatttt tacattgcag cgatgccact cttcgttcag caagagatac
    24001 tgttcgttag acatgttagt ggaaaaatcc tccgtaagat ccgttctgct acgatatgta
    24061 cgaaaaacat cgtaattatg cccatcgatg agaatattgc tttcagtcga tcgtggatac
    24121 cccagaatta cttttttacc cgatgaatag agatactaaa tgagcaagga ttttgtatac
    24181 tcagaatacg tcatacataa tacgtatgat gacttagatt tccggaggta gattaagaaa
    24241 tggccaggcg taaatccctt tacgagatca tatattcaca gatagaacag aaagtccagg
    24301 agatgggtga tggaacacaa ctgccatcaa tagaaaagct ttgtgaagat tatggagcca
    24361 gcaggacggt gataagggaa gtgattactg ctcttgaaaa agagggctta gtcgttagaa
    24421 ggcaggggct cgggacattc gttgttaagg acagcggtct ggtccataca gggatcgaat
    24481 atctgagagg cttgactcgg atcatttcga gctcgggaaa gacacccaat ctgatacacg
    24541 acagatttaa agaggtcctt gctgatggcg acctagcagc gaagctggag atgagggaaa
    24601 aggaaacact tgtccttacg gaaagggtgt acgccgcaga cggtattcct gcaatattcg
    24661 caaaaacgta cattgcttcg gaaagaattc ccggaggcac agaggaatta ctcaagactc
    24721 tcggagaaag aagggcgaag gaattggttc tctttgatct tctggaggag aagttcaaag
    24781 atccaattag atatgcgatt gcggagatcg aatctaggct cgtgaacgaa gagcttgcgg
    24841 acctgctcga aatgactccg ggcacttcga tagtgctgct caaagaagtt caccgggata
    24901 taaacaacgt cccgatgctt tactcggagg atttcataaa tacgtcggtc ttcagaatac
    24961 atgttcttag aaagaagatt tgaggtgacg ctttgatgga ggattacaga gagccagtag
    25021 gattagaggg aagatttcag tatcacattg agtgccgcac ggattgactc ggatcctcca
    25081 aaaccccttg agtccaatac actaccgact ttcttaaact ctcttgagaa gtcgctgctg
    25141 tctctttcca gagcttcaaa ggctctcaaa ataaccgcat catcacttcc aagacccctt
    25201 tccagcagga agcggatcga cgcctccata ctgtcttccc cgtggaagct ctcaccaagc
    25261 cagccatctt gtttctggtt ttcgattatc ttcttaacac tggtatcatc gaggattccc
    25321 gaatgaagta ctctcatatt cagagtatcc tgactctctt ccaggatttc cttttggacc
    25381 ctgtatttta tagatgaaca ggcgtctcct tcgagaagaa aggaaatcat tatctctctt
    25441 agcatctcta cctccgttca ttgatgacga aatgcccttc ggaattcgat ggtgttatgt
    25501 acgaaatact acactttact cagttcgtat agcaagtatc gataattgag atgaacggtc
    25561 aaaggggttt cagtctgttc ttcatctctt cctttcctag attgacgcat gatagtaaac
    25621 ctgccctgag gtgactgaac aggtataata aagctaatga ttgcagtcta ggattggtga
    25681 tctttgaagt gattgaaagc ctaggtacgt atagctactc ggcgtccacg gaagagcttc
    25741 gaatcggaga gaagatacaa cataaagcaa aaaagcgcat ctcgaagaaa gggtgagcat
    25801 atcaatgaac ctacttctcg aaaaactcaa ggaagtgctg ttagccgttc ttcccattac
    25861 ggtgctggtt ctggtgctca atttcctgtt tgtctcgttg agttggtttc taatattcag
    25921 atttgtgatt ggctcctttt tgattgttgt cgggctgtct gcgtttcttt ttggagtcag
    25981 tatagggatt agtcctatcg gtaacttcat gggtaccttc atagcaaaga ctaataagct
    26041 ctggatagta gctgttgcag gtcttgtgct tggattcttc atatccatag ccgaacccgc
    26101 actgcatata cacgcgcttc aggtcgagct tgtctcttca ggtatattat ctagcgggag
    26161 tgtcgttata actgcatcaa ttggcattgc agtttttctc tcactcggac tcataagaat
    26221 cgtcttcaat gtcgccctca agaaacttat gacagtgtct tacggtataa tacttatcct
    26281 ttctctcttt tcttcgagag aattacttgc gatctctttc gacgcttccg gagctaccac
    26341 aggcgcgatg accgttccat tcattgttac acttgcagcc gggatatcag ttctcaagag
    26401 agatagtaga tcttcagaaa atgatagttt tggactcctg ggaattgcct ctgccggagc
    26461 aattatctct gtgcttctcg tcggtttagt atccactaac aacactatgg aagctgcccc
    26521 tgtttatttc gcatcggaat cttcagagat tcttggttac ttcttcgatt tgatgccaag
    26581 gatcgcgagt gaggttcttc tttcactgct tccgatactg ctggccttct tgctgcttca
    26641 gtttagctca ctgagacttt caaagagaat gctcagacaa ataactgtcg gacttctgtt
    26701 cacttttgta ggtatggtga tttttctcgt aggtgtcaac gggggcttca tggatgtcgg
    26761 gagagaaatc ggatacggca tagcctcaat gggaaacaag tcaatcttag ttctagttgg
    26821 tatgattctg ggtcttgtca caatccttgc agagccttcg gtttacgttc tgactcatca
    26881 aattgaagat gtgaccagtg gtgccgttaa gagaagaata gttcttctcg ccctatcggc
    26941 cggtgttggt ctggcagtgg ctctttcaat gattaggata gctgtaccgg cggttcaact
    27001 ctggcatttt cttctgcccg gctatgccat ctccatagta ctgtcgtttt tttcgcctaa
    27061 gctctttgtc ggaattgctt tcgatgcggg aggtgttgcc tctggaccta tgacgggtac
    27121 ttttgtaatt gctttcgctc agggtgccgc gagcgcaatt gaaggcgcga acgtgctcat
    27181 tgacgctttc ggtctcatat ctatggttgc aatgacaccc attatcgctc ttcaaatact
    27241 ggggttgata tacaagatta agacaagaaa aagaggtgtt gagtcaagtg gctagtaata
    27301 agaatatcag tccgtataag ttgatatatg cggtcgttaa ctttggaaaa ggaagcgaag
    27361 tgcttcgaat tgcaagggaa aacgggatta gcggaggcac gattctgctg gggaggggca
    27421 ctgtaagaag tggattattg aacttcttcg cgctgtacga aatccgaaag gaaatagtat
    27481 tgatagttgc aaacaatagg actgccaaga gcttgctcga agctcttgac agagaaatga
    27541 tgcttgatcg cccggatcac ggaatcgctt tcactgtaga cattcaggaa gtagaaggat
    27601 caaaagaact gggatgcaat tcgctccaag aaggaagagg tgaaaaagag attatgtatc
    27661 aattgattac agtaatagtt gataggggaa aggccgaaga agttatagat gctgctaccg
    27721 agatgggttc gaaaggtgga acggtaataa acgcgagagg atccggcgtc cacgaaagaa
    27781 gcacgttgtt cgcaatggag atcgaacctg aaaaggagat cgtcatgatc gtctccaaaa
    27841 aagagttgac acagacaata gtgaacaaga taagagagaa gctgagaatc gatgagcctg
    27901 gtaagggaat cattttcatt caagacgtgg gcgatgccta tggcctcctc aagtagagaa
    27961 agctaaacca tgcactgatg aagaggcgcg ctcctgaaag ccagtatgtt catctttggg
    28021 tcgacgctac ttgctgattc ttctagccac gggccttgta gaatgaatca aaaccaaccg
    28081 cagcaaggat tacaagtcct ttgaaaacgt actgaacata caggtctatt ccaatcaggt
    28141 taaatgcatt ggttatcatc gtcagcaaaa gcgctccaat gagagttctt actactgacc
    28201 cctctcctcc tgaaacgctg gttcctccga ttactgctga agctatcgca tccagttcgt
    28261 agccctgacc ggctagcggt gttacagaca gaagccttga agcgtagact attccggaca
    28321 tcgcagccat taaaccacct accacaaagg ttaaggtcac atacagatcg actttgattc
    28381 ccgataatct ggccgcttct ttgttacctc ctatggcaca tgagtaacga ccgaactttg
    28441 ttttaactag aacaaactgg cctagagcca caaggacaag aagaagaatt atagggaagg
    28501 gaatcggccc aacgtatcca tttccgatga aagcaaaccc ttcagagtca acgtaaaggg
    28561 ggtaccctcc tgtgtacatg tacacaattc ccctgataat tgtcattgtt ccaagagtag
    28621 caatgattgg cggtatgtgg atctttgaac tcaagaaacc attagctgcg ccgaatgcgg
    28681 cgccaacaag caaaaccaca acaactgcaa gaaaccagtg catcgaggat tggagcccaa
    28741 ccgccattgc agctgtaagg gcaagtagcg aaccaactga aatatcgaat cctccgccga
    28801 tgatgacgaa agtcgaacct atcgccatta tcccgataat cgagctctgc ctgaatatgt
    28861 ttatgatatt tctccaagat agaaagttgg gtgtcataag tgagatgacc agcgccaaca
    28921 caaagaatgc tatgatgatt ttgtactttc ccagaaagct tgccaacttc gacttaacag
    28981 cgggtcctga cattcaactc accacctgat tatctagatg ctcttgtttt ccgctcaacg
    29041 ccttcttcat gatctcgtcc tggcgagctc cgcgtttgaa ttctcctacg acctctccat
    29101 ctgcgattac gaggattctg tcggatactc tccgaacttc cggcacttca gaggaaatga
    29161 agataacgga gaagccgttc tcggcaagtt ctctcacaat cgagtagatc tcactctttg
    29221 ccttcacatc gattccctgt gtcgcctcgt ccatgaccag caatttcgga tttgtcagca
    29281 gccattttgc aattacgact ttctgctggt tgcctccgct aagaaacttc gtctgctgat
    29341 gtatcgaagc aagccggatg ttaagtcttt ccctgtattc attcgcttta acatactctc
    29401 tcttcgtcag gatcattccg ggcatcgcag tgaaatcgct tatcgaaggg agagtgatat
    29461 tcttgaagac ctcgaaaagc ataatgaggc catcggattt cctgtcttcg ggtattagcg
    29521 ctatccctgc atttattgca tctaccggcg agcttagatt tactctcttg ccctcgacta
    29581 ttatctcgcc gctgtctgcc ttctcggcgc cgaaaatcgc cctggccaac tccgtttttc
    29641 ccgagccggt gagaccggta attccaagca cttcaccggg atagacttcg aatgagatat
    29701 cccttactct aggcaacttt tgaatgttct ttacagatat gaccggtgga aggtcgtagt
    29761 ttgcagaact cgaaatatct tcgatatcat gaaagacttc accgaccatt agatttacga
    29821 tctcttctct gtcagtctca gcggtattca gagtggagac cttcctgccg tctctaagga
    29881 cagtaattct atcggtaatc ctgaaaacct cttccaaaat atgggtgata tacaggatcg
    29941 aaattccctt gcttttgagt tgagagatga tcttgaagag atgatcggac tcttccttcg
    30001 aaagcgatgc cgtcggctcg tccatgatca ctaccctcga attcctggaa agtgccttca
    30061 tgatttccac taccttctgc tgcgccaccc cgagcctgaa tatcttctcg tccggtgaga
    30121 gatcaaatcc atacgactca caaaggctgc gaaatctctt tctcatattc ttttctgaaa
    30181 gcaggcccga gacctgcatc tcgtcaccga ggaatatgtt ttgctcgacg gtgaatttgg
    30241 gaatgaggct cctctcctga tagatgacgc ttattccctg cctgaaagat tccgttggag
    30301 atgagaagct ggtttccttc ccgtcataga gaatggtccc ggaatctttc tgataagctc
    30361 cgacaatgca ttttgtgagc gtcgactttc ccgatccatt ctctcccaga agggcgtgaa
    30421 cctccccctc tctcacatcc atgtctaccc tgtcaagagc ctgaacaccg ggaaagctct
    30481 ttgagatttc cttcacttcg agaatcagtc taggcccgac agtatcttta ttcaaagcga
    30541 tcacccacat actcgagaat cgcatcactt atgtgtctta tatcccagga gagaacaaac
    30601 ccatcatggc cttttctaag agttgcatct acgtaattcc tgacttcctc cagacctacc
    30661 gaacatacag ggggaagatt gaccgcttca aagccgggaa aaaccttcac tgacgggctg
    30721 aggctcattc gaactatctt atcaagctcc aatgagaaat tgctttgatc gatgccgtct
    30781 gcaattagtt catcaaaact ccctggtatt gcgagaccga caaattccga aatgatcttc
    30841 agtgctacct tttcactaaa ctcaggtact agcttcataa gagtctttgc aagtgagaga
    30901 agttccatcg gaagcccggc tggtcccatc gtcttacagt agatcatagg ttttatccag
    30961 tcaacttctc tggaaatctg tccgtagtcc tgagatacaa aatccgccag ggaaggggaa
    31021 aatagatcta ctccaacttc aagtcccttt tccctggctt tccttgaata ctccgaaagc
    31081 aaattgagaa ccgaagagat ccgaaattct cgaaatcccg ttgatgtctt tcggaattct
    31141 gtgatcgttc taagaacctc gctactaatt ctctcagtat ttagttcttc aatgaaagcg
    31201 cagaaggtct tttcaaaatc cgaaacgaga ccgtgggact tgactttgca ggtgtcacag
    31261 aagcaggtaa gcatctctcc tataccgttt gctggagagg ggaaacgaat tctatccagg
    31321 aagacgccgt caaaatcagc cagatttatg atctcttcaa aacggtccag aaaggctggc
    31381 gcttcgccaa ctctattcgg gcaaagaaat ttgaattcct cgccaccggc cttttggtaa
    31441 tcaagaattt cggattcgct cctctcaata atcccagtca cttcgaaggc agatgtatcg
    31501 aaattcaccg gcgtatctgc cagcaacgga taccacagat gtggggatac tccggcgcct
    31561 ctacagatcg aaacgacagg acgaaggaat ctgggctcta cagaccaaat catgagcctt
    31621 tcaagtccag atttctttgc tattgtcagt ttgtctttga gtagtcggga attcacttca
    31681 gtaaactctt cgttcagaaa gagctgagag aatatcctgg ccttacctgc ttcgtgcacc
    31741 tgtgacctcc taaagtgttc gcttacattt ccggctcgaa atcggctacg ttatcctttg
    31801 taaccattac ctgctggatc tcgataaact taggaacttc ctgacccgta agataaagat
    31861 gtgcagcttc aacggctcta tagccttctt cgataacaga gaagtatgtt gatgcgtaaa
    31921 catctcccgc ttcgatgtaa ggaacgagtt cctttgcata ttcaaggcca agaacgacca
    31981 catctgttat tcctctttct tttagggcca tggaggctgc tatggctccc gaccaccact
    32041 gacatgctat gacatcaacg tttctctcac tttgcaggaa agcttccatg aaagcaagtg
    32101 ttccttcctt tgtccagccg tcaatcggtc gcttctcgat aattctccag ttggggtttc
    32161 gttcagccac tgagaggaaa ccttcttctc gcatcctttg agccgtggtg ccgggatttc
    32221 cttcgataag aattatgtct aattcgacat ttccaaatag cttctcgaca aattcagcct
    32281 gaacaactcc cgatccaatc tcggaacgac caatgtgact aacgactccc ggatactttc
    32341 cttcaggatc agttccaatg ctgttcgtaa aagtaataat cgggatgtct gcctcattgc
    32401 acctggtgac ggccgttctt agagccatat tgtctgccgc acaaagcaag acaaggtcga
    32461 cgcctcgagc aatgaagttt tcgacctgtg atagttgttt accggcatcc cattcagcat
    32521 cggcataaat cagatttaga tcgaattcct ttgcggcttt gtcaagccca attctctgaa
    32581 cactaaagaa ttcaaccgtt cccggaagta gaacggcgac gtctgttgca aataataatc
    32641 ctgttaccaa tccaaacaca atgactaaca aaacggaaag ctttttcata agtacctcct
    32701 ctggattgaa tcctgcattt caaaatcctg aagcaccccc aagcacttcc ttaaggagta
    32761 actccttctg ggcaaaatac gccccaccga taacgacact gtaaggttcc aatgaagctc
    32821 tcgcaagaag atcggatctg tatagctctt ccggtaaaac tctcctgagg gtgtcgttaa
    32881 taatcggaaa aatccggtca tactggttga acccaatacc acctcctatt agaagtctgg
    32941 ctggatcaag aagcataata gcatttgcaa atgcgagagc gagatgttcg cacccgctga
    33001 ctattctttt gctgattcta tcgtccgact ccatattttc aaagagctcc ttaacagaga
    33061 ctttcatacc attgcttcta cagaaggatt cgagtgcata tccggaaaac cattgctcaa
    33121 gtctgccaaa tccaatattc atctttgatt caattgacca gtctgtaaca aactcgccaa
    33181 tctcacctgc tcctccagaa gatcccctgt aaactgaatc ggcgataact atacctgaac
    33241 caatacctgt tccgatcgac acaagcacta catcgccaaa ctcttttgct gaaccgatcc
    33301 acgactcccc gaatgcggca agagtaacat cgttttcgat caacgtcggt ataccgaagc
    33361 actcttcaac atgacttttg agatcgaaat ttcccatatc gaatgatggc atatacttga
    33421 tattctgggt attcctatca actgtaccgg gaatgccaat ggaaattcca agaacctttc
    33481 ctctatcgac ctgcgcgatg taactctcaa ttctttcact gagttcatct atcaagcctc
    33541 ttctcgttcg aagagcatcg gaaggtatgg tctcgctatt tgtgatgttc ccatctagat
    33601 ctataagtgc aaagatggtc tttgttccac ctatgtctat gctgagtacg tatctgtaat
    33661 ctttcgcaaa ggaaatgttg ataggtttcc taccaccgga agacttgctt tttccaagac
    33721 cgttctcagt aatcacgcct tccgcaacga gagcgttgac tatcgaagaa atcgtcggtt
    33781 tgcttaatcc tgtaagctta gcaagctgaa ctctggaagt gctctctctt cccctcacaa
    33841 ttcttaagat ctcatctgtt ctgctctcta taagcctcga taccactgga tcctcctgtt
    33901 agcattcaga gttatttttc aatagtattc taaaagcgaa ctcagaaaga acttgagaac
    33961 cacattgatc atgatctagg ttggtaaatt taaattacta actaagtctg cctaaattat
    34021 atcccgttaa ggagtcagat caaaggagag aacacttaac caatccagat catacaaaat
    34081 ggactacagg attaattggc ctgcgccaaa gcttatggaa cgtagtcagg cttcacaatt
    34141 gttggaaata taaactcaat atttgcttct gcgaacagtc tcttcctcct ggagttcgtc
    34201 tgcaagaaga gtggagagaa atctgcattt catacgtcct tttgaggctt tcagctcttc
    34261 tactcaaaac tctttcttga atcgtttttt ttggcaccaa tagcttaatg gccgaacttg
    34321 tctgctctcg aagtgtctac ctatgaactt gtaggagtcc tcttgagagg acttacaata
    34381 ttggctacag aaaagaataa tgattctaat cggtcgtggc cagtttagat cacgcctctt
    34441 cgctcggcag aagaagtcga aaatagatcg caattttgta tactcagaat acgtcataca
    34501 taatacgtat gatgacttag atttccggag gtagattaag aaatggccag gcgtaaatcc
    34561 ctttacgaga tcatatattc acagatagaa cagaaagtcc aggagatggg tgatggaaca
    34621 caactgccat caatagaaaa gctttgtgaa gattatggag ccagcaggac ggtgataagg
    34681 gaagtgatta ctgctcttga aaaagagggc ttagtcgtta gaaggcaggg gctcgggaca
    34741 ttcgttgtta aggacagcgg tctggtccat acagggatcg aatatctgag aggcttgact
    34801 cggatcattt cgagctcggg aaagacaccc aatctgatac acgacagatt taaagaggtc
    34861 cttgctgatg gcgacctagc agcgaagctg gagatgaggg aaaaggaaac acttgtcctt
    34921 acggaaaggg tgtacgccgc agacggtatt cctgcaatat tcgcaaaaac gtacattgct
    34981 tcggaaagaa ttcccggagg cacagaggaa ttactcaaga ctctcggaga aagaagggcg
    35041 aaggaattgg ttctctttga tcttctggag gagaagttca aagatccaat tagatatgcg
    35101 attgcggaga tcgaatctag gctcgtgaac gaagagcttg cggacctgct cgaaatgact
    35161 ccgggcactt cgatagtgct gctcaaagaa gttcaccggg atataaacaa cgtcccgatg
    35221 ctttactcgg aggatttcat aaatacgtcg gtcttcagaa tacatgttct tagaaagaag
    35281 atttgaggtg acgctttgat ggaggattac agagagccag taggattaga gggaagattt
    35341 cagtatcaca ttgagtgccg cgcgggagat atcgcaccgg ttgtaattgt tccgggagac
    35401 caggggagag tcgataagat cgtagccgga ctgagtgaga caagaaagat cgctgaaaac
    35461 agaggcttaa taacctatac aggtaagtat aaggattacc ccgtttcagt gacttcaaca
    35521 ggcatgggag gcccctctgc aagcatcgta tacgaggaac tgatcaacgt tggagcaaga
    35581 gtattgataa gaattggcag tgtggcaggc ctccaggagc atgttaatga aggagatatt
    35641 gtagttcctt atggctgtgt gcgtgatgac ggggcctcga actactatgt cccggcgaat
    35701 ttcccggccg ttccttctcc agatgtctat tcatcgctta caacatctgc cagagaaagg
    35761 ggaagagaga tcgtcaccgg aatcaactgg acacattcct gtttctacaa acgggatccc
    35821 gaatacttcc agagctggag caggagaagg attgtatcgc tggaaatgga ggcttctgcg
    35881 ctattcgtca tttcctatct tagaaatgtc aaggccggtt tcattgggat ttgctatgcg
    35941 aacagataca aacaatctgc tggcacaaaa gtagatctct ctgtcaagaa ccccagaaga
    36001 gacgtgattg agaattcagt gaaagaagcg atcgaaataa cgctgaccgc aataaagaaa
    36061 atgtacgatg aagaccttgt ttaactaccg aagggaggag attctatgag aaccaggttc
    36121 atgcttttgc tctttgttct gttgatagct tcatgttttg cagttccttt gcgtctttca
    36181 agtgaagttg gaattcacac ggatgcctgg aagaccagaa tggaaggttt cacgaaagag
    36241 acgggaatcg aggtcgacat tcaacagttt ccttatgcaa actaccttga tcagctgatg
    36301 cttggctaca catctgggag ggtcgaaatc gatgtacctt atatctcgat gctctggtat
    36361 ccggctcttt cgattgcaaa ctacatctac ccgataagcg atattcccgg atacgagaaa
    36421 atcaacgagg ccgatattcc cggaataaga aacgcaaagc ttaatggcaa aacgtacatt
    36481 gttccctaca tgaacgagct cggaggcata atctacagga aggatctttt cgaagatcct
    36541 gtggagaagg ccaacttcct caataagtac ggctacgagc ttctgcctcc tcagactctg
    36601 gagcagtaca gggacgttgc agagtttttc aacagaccgc cggatctcta cggagtcacc
    36661 atgatgggaa gaagaagcat attccttgca actcacttca tgcaaaggct ttgggcaaaa
    36721 ggcggggcac ttctagatat gaatatgcgt ccaatcttca actcggaagc gggcgttgaa
    36781 gcgcttgaag aagtcaagta catgttccaa tttgcgaacc ctgccgcaat gaactacgat
    36841 ttccaggaag cgttgaatga attcatcagt ggaagaagcg caatggcgga ggtctggacg
    36901 accggaatgt tctacgtaga ggatgaatcg agatcctcca ttgttggtaa ggcaggattc
    36961 gtaggattcc ccagacctga agaaaaactt ggagagaagc ttcccatgct ttatatctcc
    37021 tggggtttct cagtcagcag ctcggcacca gacaaggaag cagcgctgga gtggcttttg
    37081 tatgtcacag aaacaaagaa cgaggtcgag gccgctccta caggcaacat accggcgagg
    37141 ttctcggcgc tgaacagccc cgagcttgca gagtccttcc cctggatagg agacttcgcc
    37201 tctgctatgg agaattgtat tccaactcct atcgttccat tgataccgga aggcggaagt
    37261 atagtgagcg gaattatcgc tccagcggtt tctgagttcc tggctggaac aaagacggcc
    37321 gaaaaggctc tagatgacgc agtaaaggaa gtagacaggc tcatgagaga tggagggtac
    37381 tattgatctt attcaaagcc gggtcgaaag gcccggcatt tcttgcgatg ggggatcgga
    37441 tgaagtctaa acagaaaact aagggttttg catatattct tcctgcactt gtattcatcg
    37501 tggtgatctt catgatccca cttacataca ctgttgtcat gtcctttctt aggtggaatc
    37561 tgctcagacc cgatctggga atcaagccgg ccggtttcac gaactacgta aggcttttca
    37621 cagactcctt cacgctggac acagttggaa gaacgttcta cttcgtagtg ggagctgtcc
    37681 ttgttgaact cattataggg ctgtgtatcg ctctcgcgct tgacactgag ttcaaaggct
    37741 ggaagatagt gcagtcgatt cttcttgtac ctttcatgat cgcccctgta gtcgtaggat
    37801 acgtatggag attcgtgttg aacagcgatt atggaccaat tatccatttg atgaaacaaa
    37861 tagggtttgg cggacttgta gataaaccac tgctttctaa tccctcagcg gccatgccag
    37921 tactgatagt tgcagatgcc tgggaatata tacccttcgt taccctggta ttgcttgcgg
    37981 gtctgaagtc tgttccatat gaaccgtatg aagccgcatt cgtcgacggt gcaagctcta
    38041 ttcagagatt tttctacata acccttccgc tgctaagacc ctcgatactc gtcgcggtag
    38101 ttatcaggac gcttacgtcc ttgcgtgtct tcgacattgt gttcattatg acaggcggag
    38161 gtcccgggac agcgacggaa acgcttgcct tctacggtta caggacggcc tttcaagcgt
    38221 acaacgtcgg cttttcttcg gcaataaaca tgctcacttt cggaatagct gtggtcttca
    38281 caatactcta catgaaaatt ataggtggtg gaaagaacta tgattaggag aagacctgtc
    38341 accagaatac tggttttcgc tgttattgct ttggctctta tctggactct ggtgccactt
    38401 gcgtggattc ttctttcctc gttcaagttt gaagccgacc agttctctat accaccaaag
    38461 tggttcccag aaagacttac gcttcagaac tacgcgaagt tcttcgggaa ttctgagttt
    38521 gtgagatcat tgatcaacag cgtcatgatc acattccttt caacggtaat cgccctgttc
    38581 ctgggtgtgc ccgcagccta cgctattgcc aggttcaact ggaagcatgc caatacaatc
    38641 gctttcgtag tccttatagc aagaatgaca cctccgatag tcatggtcct gcctttcttc
    38701 atgatcgcca gatatcttgg aatatccaac acatacctcc cgatcgttct cgctctatcc
    38761 tttttcagcg tccctttcgc gatctggatg atgcgtggct tcttccagga aatccctgtt
    38821 tctcttgaag aagcggctat gattgatgga tgcacccgtt ttcagtcttt aaggagagta
    38881 gtgcttcctc ttgtaatgcc aggactatca gccacatcca tactctgcgc tctgattgcg
    38941 tggaatgaat tccttttcgc acttgtactc accggtcagg atacaagacc tcttcctgtt
    39001 ctggtcaata tgttcgtaag cgaaaggaat attgaatggg gtgttatgag cgcggcagcg
    39061 ataatcacag tgcttccgat gattgtcttt ggactccttg tccaaaatga cttggttcga
    39121 ggattgacaa tgggcagctc taaatagacc cttctcaatt acctttccag acctgctgag
    39181 actcttccca gtgcgaacgc agttattcat gcactttcgt gaaacaaagt aagaataaat
    39241 agtgtctccc tgcctcatgt gtgataattg gtcggtgtaa agaaagtctt taacaaggag
    39301 gtcagggttg atgccaagga tgcttgtggc aactgcaccg cgaaaagcgg ccatcgttga
    39361 gtatcaggat agaaagatag cggccaacga ggtgatggtc gaggtcgagt ttgcttctcc
    39421 aaagcatggc tcagaggtag cagacttcag aagtgaaagt cctctgatag acgaattcta
    39481 cgatcaagat tggcatctga ttttaccgag atcagctggc gaaaaaaagg gtgtagaatt
    39541 cggttcctgg aatctgggca acatgtgggt aggaaagata gtagtgaaag gggaagacgt
    39601 aaaagactac tccatcggtg aaagggtctg ctcttacgga ggaattcgtg aaactcacat
    39661 tataaacgct ataggaaacg accgtctact aaaggtccct accggggtct cctggaagag
    39721 cgctgtgtgt tatgatcccg cccagtttgc tttttccgga atcagagacg ggaatgtaag
    39781 gccgggagat tacatcgcag tgattggcct tggagccatc ggattaatgg cggtacagct
    39841 cggaaagaaa atcggagcca ggccagtgat aggtgtggat cctatcgcga taaggcgtga
    39901 ggctgcgctg aactccggag cggactacgt cctcgaccca ggtgagtgcg acatcggtct
    39961 tgagataaaa aagcttaccg gcaagctcgg ggccgactgc atcattgaga ccagcggatc
    40021 tgctggcgcg cttcagtcgg ctctaagggg gcttgcctac ggaggaacgg tttcatatgt
    40081 ggcctgggca agggagtttg ccggcggcat caacttcgga agagaagccc actacaatta
    40141 cggaagagtt gttttttctc gcgccgcgag tgaacccagt ccagactttc caagatggga
    40201 tagaaagaga atcgaaaaaa cctgctggaa tttgatactg gatggttctc tatctggcga
    40261 cgatatcgtg gatccagttg tgaacttcga agaatcgccg agcgcgtact gtaaatatgt
    40321 ggacactaat cccgaactga gtattaagct cggagtagct ttttgagagg tgagtatatg
    40381 aaagtgataa ctcaggacaa agatttcttc ccttctcaaa tgaaagagaa actgcaattc
    40441 attcgaagcc ttggattcga cggttacgag atagacggaa aagatctact ggaaaggttc
    40501 gcggaagtga gcgaagctgt ccggtcaaca ggattccctg taacaagcgc ctgtggggga
    40561 taccgtggtt ggatcggtga cttttcccgg gagaaaaggg agcaggcagt agaggacatc
    40621 ggagagatct tgaaggcatt atctaaggtc ggtggaaagg gaattgtcgt acctgccgcg
    40681 tggggaatgt tttcgaagag gcttccaccc atggtccctc cgagatctga ttcagaagat
    40741 acagcagttc tcctggaatc ccttgaaagc ttgaacaaaa tggctgcaga aacggacacc
    40801 ttcatctatc tggagccact taacaggtat gaagatcata tgctaaacac ccttcaatct
    40861 gccgcaaaac tgatagacgt cggtggattt tctaatgtca gacttacggc tgacgctttt
    40921 catatgaata tcgaagaggc cgatatcagg caggcggtaa tagagaacgg caggtatatc
    40981 ggacacgtgc acatcgccga cagcaatcgt tatcagccgg gtcaggcgca cttagacttc
    41041 aagacactct tttcggcact gtctgaaatc ggttactccg gtgacattgc attcgaatgc
    41101 agagtgatcg gagacaatcc tgctaaaaga tacggtgagt catgcgcctt tattcgtgat
    41161 cttctcagga gcattgaaag atgaagtacg ggaaactccg tgtcgccttg attggggccg
    41221 gccagattgc agaggtcacg catctaccgg ttttatccca gatgaatgac agggtagaac
    41281 tggtcgggat agcagatccc gctgtagaga gggcagttat gctttccagc agatttggaa
    41341 tagataatac tttcggtgat tacgatgaga tgctgtcttc tttgaatccc gatgctgtgt
    41401 tcgtttgcac tccaaacaaa tttcactctt cagctactgt tagcgcactg agatctggat
    41461 ctcatgtctt ttgtgaaaaa cctcccgcca tcaacgcaag agaagttcaa gaaatgatgg
    41521 tcgcctcaaa ggaagcagga aagattcttt cattcaactt ccattatcgt ttcaggagtg
    41581 aagcaatcgc cataaagaag tttgtcgaca gtggagatct tggtgagata tatttttgcg
    41641 aagcaacagc gttgaggaga cgcggaattc ctggatgggg ttccttcacc aacaaagaga
    41701 tccagggcgg cggtccactg gtcgatatag gagttcatat gctggatctg gcgctccata
    41761 tgatgaaatt cccacagccg aagagcgtta cggcaagcgc ttattgtaaa atcgccaaga
    41821 ggaatggagt cggacttatg ggcggctggg atcctgagaa gtattccgtc gaagattcgg
    41881 cctttggctt tgtgaggttt cacaacggag cttcactgat cctgaagact tcctttgcgc
    41941 taaacatgaa agatcgttca atcatgaatc ttcaaatttt cggtgataga gcgggcgcta
    42001 cggtcttccc accggaagta tttgccgaat cgcatggaga gttgacgaat acggctatac
    42061 catatgcaga aagcaaagac tgctacaaga ggtcgataga aagctttata gatagttgcc
    42121 tgggagagga atcgagaata tcctctcccg aagaggccct ggttgttcag agcatcttag
    42181 acgcctttta tctgtcggcc gaaagtgatt cgacaataga gctataaacg gaggtcctcg
    42241 tcatgagaat cggcattctg aacggtgcac gataggtaaa gaaaaattcg atgagtacat
    42301 tgacagacta gttttttctc ttcgggatag aggatacgat gtcgagtatt tcttgctcag
    42361 ggatcttgat ataaaacagt gcatgggatg ctttggatgt tgggtagaat caccgggaga
    42421 atgcatcgtg aaagatgact cctatgagat caggaaatct gcagttaatt ccagtctcct
    42481 tctatttgca tcaccactca tattgtgtct cacttcgtcc acattggagg aggcgatcga
    42541 taagatgatc ccaatagtcc ttccgttttt cactgttggg gcaggcaaaa tccatcaagg
    42601 aagaggtacg ggaagtattc tttgcgagtg ttctgactta agaataggac gatacagcca
    42661 tagtcgatct tgagatcgtg ggaagatccg cgaacgcact gctaaagaaa tggcttcaat
    42721 cttgcctttt ttgattccac cgagggaacg ttagagaaga tttcagatga gactgctggt
    42781 cttcaatgga tctccgcgag aagttaagcg cgacacattg acgattgtaa accactttct
    42841 cgaagaagta aggtcatctc tggcgaggaa gggttattga gattgtggac cctagcagga
    42901 agcagaaaat aatcctctca ttacatatat tatgatgtat aacgaggaac aaacgtggag
    42961 aacttcatga taagtcctgt atgcttttca ggcaagtccg tttgcacacg gtattttgtc
    43021 ggcgaactac aggtctttaa ttatcaatct ctgtgtctta tgacgcctgc gagcttttcc
    43081 aactcaatca ccggtatagg tacaagagaa agcagaaaag caagcaaata catcgaagca
    43141 ttcaccttgg ctagcccgaa tattctcgct actggatcta tgaatagaac aaaagcaacc
    43201 attgccagcg acactactgt ggcaaaaata tggaagacat tggaaaagaa accggtttta
    43261 aacagcgagt gatttgaccg catgttaaaa gaatggaaga tctgggtcaa agaaagtata
    43321 gcaaaggcca ttgttctgcc tacaacaata tctccggtac tcttccagcc aatcgaaaac
    43381 ccaatcagag ttagaaaagc gaacatgatt ccctgcagaa ttactctgta accaagtcca
    43441 tttgcaaata tcccttcatt ctttcgttta ggcttgcttt tcataacatc ctcttcaacg
    43501 ggttccatgc ctaatgcaat tgcaggtaag ctgtcggtga cgagatttat ccagagcaac
    43561 tgaacagata ttagaggagc ttctctccag ataatcatcg ccgtaaatac agtcaaaatc
    43621 tcccctatat tagttccaag aaggaaacca accacctttc ttatgttggc atagatcccc
    43681 cgaccttcct tcacggcctc tacaatagtt gagaaattat catctgtaag tgtcatgtca
    43741 gccgcgctct ttgccacatc ggttccagtt attcccatgg cacatcctat atctgctgcc
    43801 ttgagagcag gagcgtcatt aactccatct cccgtcattg ccaccacttc accttgtttc
    43861 tgccatgccc gaacgatcct caacttgtct gaaggagaaa ctctggcata aaccgagata
    43921 tcccgaactc gttcttcgag ctcactatcc ggcatttttg caagttcgct tccatcaaca
    43981 accttttctt caggtgacag aatcccgact tctctggcaa ttgctgcggc ggttagcttg
    44041 tgatcaccgg ttatcatcac cggctttatc ccggcctgtt tacatagtgc caccgattcc
    44101 tttgcttctg gccgtgctgg atcaatcata cccacgagac ccatgaatgt tagcccgctc
    44161 tcgatctcgg cgggatcaag gaactctgga atctcctcga tatgcttata ggctatggca
    44221 agcactcgca gggcatcgct acccatctct tctgcaattc ttcttccctt ttcgacgtct
    44281 cctgcaacac atctaagggc aactgaatca attgcaccct ttgtaattac tacattcttg
    44341 ttttcgattc tgttgattgt tgtcatcatt tttcgctctg aatcgaatgg aatctcggct
    44401 aatcttggat atttctcctc cattttctct ttctcgattc cattcctttt agcggcaaat
    44461 actatcgctg tctcagttgg gtctcctatt tgtttttctt ctccactttc ataaatgatt
    44521 cttccgtctg aacagagtgt cgcatacagt aaaagacttc ttatttctgg cgagttctcg
    44581 gaagagatct cttccagagc tgaattacaa tcggaaaagg ccttaaccag tgtcattttg
    44641 ttctgagtca gtgttcctgt cttgtctgag cagattacgg ttgcacttcc cagggtctca
    44701 acggctggca gcttgcgaat tatcgcattt cttttaacca tccgctgcac tcctatggca
    44761 agaacaaccg tcacgatagc aggaaggcct tcaggtattg ccgaaacggc tagagaaacg
    44821 gagatcatga atatctccat tatcggaatg ccatcaatca aaccaaccag aaagattatc
    44881 gcgcaggtaa ctatagccgc aattgcaaga tactttccaa gaacagtcag tttcttctga
    44941 agaggagttt ccgtctcttc ttccgctgaa agcatgctcg caattttccc catttcagtt
    45001 tgcattccag tctctacgac aacagccttt gcccttccat aagtaacgct acaaccggag
    45061 aaaatcatgt tgaaccggtc tcccaaagta gcatcttctt taaccttagc ctcggttttc
    45121 ttctccgacg aaaccgattc tccggttagg gctgactcat caactttgag acttgccgaa
    45181 ctcaagattc tagcatcggc tggaacgaag tcaccggctt ctacaagcac aatatcacct
    45241 gggaccacgt tcgaagcttg aacgatcctt tgctccccat cacgtattac cttcgcatta
    45301 ggtgaagaaa gcttcttcaa agcatcgaga gccttctcgg ctttgctttc ctgtattaca
    45361 ccgattaccg cattcaagac tactataagc attatcaaaa caggttcgaa gaaaccttca
    45421 ttctcgaata gagaaacaac gaaggaaatt gcggctgccg ccataaggat aactatcata
    45481 acgtccttaa actgttctag gaaacgctcg aagatggttt tctttcgttc ctctctcagg
    45541 gtgttgctgc cgaatctagc caatcgttct tccacttcag aagaactcaa tcctttgttg
    45601 ggatcagcat tcagtttctc taatacctct tctagttcca atgagtacca tgccattctt
    45661 agtatcctcc gttttctctg tgtgcaactt caaggaggca aatccatatc gcgacttctt
    45721 ggatcgcttt aaatacagac ttaactccgg gccgacgctt tcattcagta tcgtcagagc
    45781 gctttgttct gagcgaaatc tcattcccgc ttttcaaaga agacaatact actctgaacg
    45841 attgaagaaa tgagttgcgc agttcatcac cactcatcat acttctctct ttcagatcat
    45901 catagtatgc tgcaaaagtt acaaggggca ttacagagaa gaagaacttg aacaattcca
    45961 tgtccaggga gtaagtgaaa tcgtggtcgg cttcggaaat caattttagg atgggattgc
    46021 tgtcaatgtc tctcgagaga tcaagcagcc tgaagagatc gttatggtgt ttgctgtctt
    46081 tcaatgattc gaacatcaaa accctcagca acaacctgtt tcccaataga taatcgagta
    46141 tcctttcata gtattcaagt aatcggttca cgaaaacaat agcagactcc tcgttcgaaa
    46201 acttcatcct gtcatattca atatcgagtt cgccgtcttt gatcatctga atgatgcctt
    46261 cctggacgaa atccatggtg atggaagtgg catcttcaag taaggaatta actacgtagc
    46321 ttaggatctc ctccttactt ttgaagtagt aatagatcag ccctttcgtc actcctgcgg
    46381 cctcagcaat ctcacttgta gtagttgcat ggaatcctct ttttgaaaac agctctatcg
    46441 atgcatcgat gattctttct cttgcatcgt tagcatcctt tgatttcatc acaccacttc
    46501 ctaactaacc ggttagtcaa aatcattgta ccattttgtt agctctttag ggaggtatcg
    46561 agttttttag attctctctg gtaaatgaat caagagaatg gaaaggtttc gtattcaccc
    46621 ccaggggaat taaacaaata agtgaggaat agtagactaa ggagaagggg tgaatatggc
    46681 tgaagcatcc gaaaggcaag cattatgatc ctgagttcaa actcagatta gttagggagt
    46741 tcttagaggc agacaaaacc cttgaagaaa ttgcacaaga taaggggttc gtacttcaaa
    46801 acacttcaga acatcacaag acgctggatt tctcttattc gatgagtatc gtttattagg
    46861 cgggccattc acgttttcta atctctgtcg gttaaccaat ccttgcaaag gaaactgtga
    46921 aagtgcagct tctgggaagt cgagatggga aggcgagaaa tattcattcc ggacttagct
    46981 cttactatta cccgtaactt gcagtcacca ctggattctt agagaattac tagtaaataa
    47041 ctctcagaga aatagactag tttttttcgt cttagccctt caaaaaacca tgaataatgc
    47101 aataaacgca atcgcagccc cattaaacac tggggccgac gtagatcact agatgtgcga
    47161 aacttaccga gacaactcct ttcgaatcat tcaacttgaa aaatcgaaat ttccactgca
    47221 gggaaagctt gaagaataga tatgaaccga gcgatagaac ctttgacaca gtttgtttag
    47281 gaggtgcttc caaaaagcct tgtgaacagc aaaagatgga aaactacaac aaacagtgcc
    47341 tcagcagtgc tttcccgtcg tgattagaga gcacgatccc attcctggag aagataccct
    47401 gactgttgaa tgactgactc ttaccgctat gctttcccgc gcgactgctg agtaaaatcc
    47461 cgatcgatga caatcattct ttattctagc tgagtgcaaa aacttgactc aaaaataatt
    47521 caggtgtaga atcctatatg tacgtacatt ctaactggag gcgactgtta tcactcctaa
    47581 gtattcagta attgagcatt ttctgcttga agaactcaag tccgggaagt actctgttgg
    47641 agataaactt ccaacggaga aagagcttat gaagaagttc agtgcaagca gagaaactgt
    47701 ccggaaggct ctggataggc tctctttcga ggcggcaata gtcaggagac cgggccttgg
    47761 aacctttgta ggctatggaa gtgacaacca cctcgtgggc attctagtcc agcaaataac
    47821 cagttacatc tttccatata tagtgctcgg ggccgaggac cacctctttc gaaatgaata
    47881 caagatgctt ctaggcaatt cggcggagga tccagtcaag gaacggcaga tcctgtcgga
    47941 gtggattgct tccggggtaa agggtttgat aatcgatcct gtgtacagtg ctacaaagag
    48001 gtcaaataaa gatctagtga aatctatggc aaaatcggga atcaagattg ttctggtaca
    48061 cagcgactgg aacatagatc aagtgagctg caatgttctt gacgatgcct ttggaggaga
    48121 aaaagcggcg gagatcttct ttgaatacgg tcataaaaga gttgccgtga tatacaaatc
    48181 gacacacctt cccggcgtca taagggccaa gagcttcgta gatcgctgca gacaactggg
    48241 gttttcaaag atatacgaaa agtccttcaa cgtttcggag ttcacaggtg cgcctatgca
    48301 aatcgctcat gaactgatgt cgttaccgct gcagattcgc cctactgcaa tcttctgtta
    48361 caacgatgcg acggcccttc agctacagct ggtagcaaag agacttgctc taaatatacc
    48421 cgaagatatc tcggttatag gattcgatga cgggcctatc ggtgatttca gagacatcct
    48481 gaccactttt gcccacccaa aagaagatgt gggaagaagg tctgtcgaaa tccttcttga
    48541 catgctcaat ggctccaaag cttatagagt tgtccataaa ccggaattga ttgaaagaag
    48601 ttcagttgct ggtgtgaagg aatagttctg aaccgctttc tttatgaaac tcaaacttct
    48661 gatcttaaga cagatcatct gcaaccctct ttaaaggcta agcgatcatg gaatttacat
    48721 ctttggcttt cactctgtga ctatcttagt ttctcttcgt cattcgcaaa tcaacttgtg
    48781 gctctgacgt ccagagcgat tccttcttaa acaacgttgt caaaaatcgt ggttgtactc
    48841 ttcgccccag gttcttatta gtggactggt aagggttggt gcctcgaagc cataagaaac
    48901 gcaactaaga actagatttt cagaaaatat tggactagca atggcacaag aagctaactg
    48961 gcgtcgtgaa agaaaatacc cctgctgggc accgaggagc agaaatcgga aggccaaaac
    49021 aaaaaatctt ctctcatatc tgttcgaggt tcttcgatct attgagaaaa ggaacttgac
    49081 ttctctgcga gagagacgcc gataggaatc cattatagaa acgacggcta tggaatcttc
    49141 cgagaaagtt attcccaacg tcaaaatgga tttgggtttt ttccgtctca aaatacgaac
    49201 ctcttgtctc ttcctctcaa gttccaggaa cttcggtaaa gaggagagaa agtcgagaaa
    49261 tgaaaaaggg catttcctac atgttcgttt cacgaaagga atcagaaaag gaaaacaaaa
    49321 tggtttgcgc ttccaagaca gtttccgtca taatcgttgc catatgctat cccctgttta
    49381 gatcgaaata tcggacttct tcatgaatta gtccggaatc aacgctccat atatgtacgg
    49441 acattattct atgataatca cgaagaaatc aatgagaatc cgtcctttca atattcgatt
    49501 aatagaaata tgaagatatc ggcccaaacc cggctcagat catcagagaa cggctctaat
    49561 cgaatttggc gcaacttctt cgattttgct acaatgtgac cgtgaattga ttctcctctt
    49621 aataacaact gatttctaat cgtggcagaa ttctggatct gtcttttgtg cggaagtgtg
    49681 ggcaagtatg tttggttttt ttcaataagg tacgtacata tatgtatttc ccgacaaacc
    49741 agagtagaag ccagtgcttt caaacgtaat caaacaacca atttttctgt gaagagctta
    49801 actcattttt tcggtctgac tgatcaagaa atatcatttt ttttgggggg tggtatgaca
    49861 ggcaatagat cagagactag atcgttttct tgttcattaa caatctggta tttagtattt
    49921 ctaggaggtg aaactcagtg agaaagttga tggttatggt acttgcgctg gttcttgccg
    49981 ttggtttctc tttcggtata acaattacga tgatgtcacc gctgaccgga gctgatggag
    50041 cttacatgga tgaaatcgtg gcaagattca acgaggagca ttctgacatc gaagttgttc
    50101 atgtggtcgt agagtcttct ctggatatga agaacaaact ttcaatgggt attgcatcta
    50161 agacagcgcc cgagatcatg ttcatcagga aattcgacat gcctctttac ctcgaacatt
    50221 ttaagggatt cacaaaggaa gaatggcttg aaaactatgg aatcgacaca gatgatatct
    50281 tcccaggtgt tctcgaagga ctagttgtgg atggtaaagt ggtaggaatt cctctggaca
    50341 tatggctgtt ctacatggcc tacaacaagg caaacttcgc aaaggtaggc ctagatccta
    50401 acaacccgcc gaaaacgaga gaagaattca tatctgccat ggaggctttg ttgcctataa
    50461 cgcctgaagg cttgacacca tattatgaga accctgcctg gacatggatc tggttccacc
    50521 ttctctggca gcacggcggt gaagtgctta ccgacgactt caaggcacct gcgtttgaaa
    50581 aagccggaat cgaagtctgt aagttaatgc ttgagatgca ggataggggt attctgccga
    50641 tgcaggtagc cgacccaggc gtttccttcc agtcgggcga aagctcggtc ttgattaccg
    50701 gtatctggac tatcaagcca tggatggaac agctgggtga ggattttggt tatgcagtag
    50761 ctcctcagct aggcaacact aaggccgtat ttggaggatc ccacgtgctt gcaatgccag
    50821 ctgtcatggt cgaagatcca aaggtacttg aagctgcgac aacattcata aagtatcttt
    50881 gggaccacgc aatagactgg tatgaagcag gacagactcc cgcaagaaag tccattgccg
    50941 agagtgagga actgaaggaa aggcttcctc atatctacgc ggtctctcag gactctgaat
    51001 atgtgaggca gttccagatg ttcccgctta tttcagagat tgaggccgaa atcgccgttt
    51061 atctagagga aatcctcgta ctccattcga tgtccccaga agctggaatg aaggaagcgg
    51121 catttgccgt tcaggaaatt ctcgacgact attggtcgag aaactgatct gcgtaagtaa
    51181 cggttaataa ggtgaaggga ggaggagtaa ttgagacaat cacattctaa gtcaccaaga
    51241 agtttccgca gggaggttgg atcgtggttg ttcctcctcc ctcacctgtt ctttttcatt
    51301 cttttcgttg ctgtccctct agtattcgga ctcattatca gtttcttcaa ctggagtctc
    51361 ctgaaagaaa acgtattcat tggtttcaat aactatgtgc ggacatggaa cgattcaagg
    51421 ttctggccaa cggttcagca cactgtaatc tttgcactga ttagcgtccc tttggtaata
    51481 attgtggcaa tccttttcgc ccacatactc aagaaaaagc gcttcggtca gctttggtta
    51541 ctcgttgcct tcgtttcgcc agctttcttc ggatctgtag gaattctgag ttcctggaaa
    51601 tggatatttg cttcctttcc ttcgggcctt gccaactatt acctggataa agtcgggctc
    51661 atcaacaatg cggtttcctg gtttggaagc acttctgtag cctggggagt gattatcctt
    51721 gttacggtct ggtggatcgt tgggttcagc atactgctat atcttggagc tctacaaaga
    51781 atcccacccg aacagtacga atcagcaaaa ctagatggag ccggaccatg gaagagattc
    51841 atctacataa ctctcccatg gatcaagagc gtgctgtttt tcgatgtcgt tcgtcaggcg
    51901 attctggcat tcgggctctt cgatcaagcc tatatattgt ctgggggagg acccgcaggg
    51961 accactcgta cactcgttta ctatctctat ctcgtgggat ttgagcgaca ggatttcgga
    52021 agggccgcgg cgatctcctg gtatatattt gcgatcgtac tgattttcgc agtgattcag
    52081 ctagttctgg tcaccaaatc tatacgatcg acggaggggt gaaactatga cttcaacagt
    52141 ccccaaaaac aagaagaaac tcagcaaacg aataactgca gtgatagtga ctgttcttct
    52201 ttgggccttt gcagctttct ggtttgcacc gatcttctgg atgctgtcga cttctatgaa
    52261 atcaacggcg gcagccgtaa ttgaaactcc tcctcaatgg atacctcaga atccaactct
    52321 agaaaactac aagatcatct tcgcaccttc aggtggtctt tcactcatca gagcaactct
    52381 caacagtgtg attgttgcgg tgagctccac tcttgcaacg ctggttctgt caatcccggc
    52441 cgcttatgct ctatctcgat tgagatttag aggacgtatg ctcatctttt ggatttatgt
    52501 cgctgtcctg gccttccctg ccgttctctt cctaatacct cacttcttca taataaacgg
    52561 tatgggacta tctaattcct acctttccct gatcttgcct ggccttgggg ggacattcgg
    52621 ggtatttctc ttaaggcagt acatgctaga catacccaga gagctggagg atgccgcgtg
    52681 gatagatggg tgttctaagt tcaggtttct gataagcatc gtgattccgt acattaagcc
    52741 cgcgatgctt gtactcagct tgatgacatt tctcggatcc tggaacagct tcctgtggcc
    52801 attactaatc ctcagcaagg ccgacaagtt cacgctccca atagctctca taagatttag
    52861 cgcggggtgg ggagacccct acagaggaat cggtccgatg atggccggtg cattcttctc
    52921 cgtagcaccc tctttgataa ttttcattgt cttccacagg tatttgatga aaggaatttc
    52981 cctgggatcg cttggaaagg agtagatatt atgtcaggaa ttaagaagtg ttttcggaaa
    53041 accgaaggag agagttttgg aacacccctt gagagatact ttgtcttgat tcttggcctg
    53101 ctgtttctta gtctatgttt tggaggtagt gttttgccta ttgacatcga cattagtgct
    53161 ttcgaagaga gaatagaagc caatagaaag gtgccgatca agattgagat cgtggatgag
    53221 aatgggcatc ctgttagcga tgcacacgta tcggtaaggc agatgaatca cgaattcttc
    53281 tttggaaatg cccctgagta tctgatttat gcctacgctt ggacaagcta caaccgtggg
    53341 agaaggtttg gaatcaagcc cttgcctgaa gaggatttag aggtatataa aaggttatac
    53401 ctcgatctct tcaatttcgc cactctacca tctttctact ggaaggacta cgaacctaca
    53461 caaggcagaa tccggctaga cgaggcttca aagaagatag ccgaatggct taatgaaaac
    53521 ggagtgcctg ttaagggcca tactctggtt tggggcaacc ccccaagtgt aggtgttcct
    53581 gcgtggatag aagttaaggg gaagcttggc cagtgggcag aggtgagaga ggcccttttc
    53641 ttcagagttg agagagaagt agaagagttc aaagatttgg tgcagtactg ggatgttgtg
    53701 aacgaacctg ttgttcaaag ctggttcgat gctcttggtc ccgattacat tgcagaatcg
    53761 tttaggattg tcaaagagac tgacccagat gccataacgg ttcttaacga gtacggggta
    53821 cttgtgaacg agcggacaag aagagctttc atttccagag ctcgacaact gataggtgac
    53881 ggagtcccta tagacgtaat aggagccgaa gcacatatct tcaccgctca agatctatta
    53941 agccagctgc aatcactgga aagtatctat cttgctatag atgaactggc tcagctcggc
    54001 aagccaattc acatttctga atttcaaatc cctcttcccg cggttatcga tgccttcgat
    54061 gtatccataa atgaggccga agagatacag gcggagatcg caaagatctt ttacacggtg
    54121 tttttcagtc atcccgcagt agaggtaatt acctactgga atttctaccg tgcttggcag
    54181 tccggaagcg gatttcttcg tgacgacctg tcagaaaaac caatgttcta cgagctgatg
    54241 aaactcatcc atggagattg gaagacctca tttgaagcag aattagactc gtctggagaa
    54301 ttgtgtttca gcgggttctc aggagaatac gacatcacgg tagaatttga gaacttctcc
    54361 gagacagtat cgataaacgt taagaagagc gagaacaatc aattcacgct cgttcttgga
    54421 caatgatcga gggggtaatc ggatgagatc aacgacgata atcctcactc tggtctttct
    54481 tgctgtttca ctttccacat ccgctctagc gaatcccgac catgtcttga aggtagaccc
    54541 aagcagtttg ggcccagaag ttagctctct tctgtacggg atattcttcg aggacataaa
    54601 tcatgctgta gacggtggcc tgtatgcaga aatgataaga aatcgatctt tcgagcatca
    54661 agatcctgtc gaaggctggt ttgcggtctt tgaatccgat tgcaaggcta ccttttcgat
    54721 tgagactgat cggcctctca acaagaataa caccaagtat ctgagcttcg aaattcaatc
    54781 tgacgacaag ctgatatccc tttcaaatga tggctacgac ggtatacctc ttaaaaaggg
    54841 cgaacggtac ttgttctcgg tatttgttcg aagcgacgaa gagtataacg gtgaaatcaa
    54901 ggttatgctg gtcgatacta aaggtgaacc tgtggccgag aaaagcctgg gaacggcatt
    54961 tagctctgaa tgggagaaat actcggtgga attgcagccc aacgaaagcc tggatgatgg
    55021 aagactcatc ataacgatga agggctccgg taagatctct tttgatatgg tatccctctt
    55081 ccccgaagag aactggaacg gcatgaggat cgatctgctt gagatgctgg aagagctgaa
    55141 gcccgctttc gtcagatttc ctggcggttg tcttgtcgaa ggcgatagtc tcgaaaatgc
    55201 ctacagatgg aaggatacaa taggtccaat tgaagaaaga aagaccaact ataatctttg
    55261 gggctatcat cagtcgtatg gaatcggatt ctacgagtat ctgcttcttt cagagcacct
    55321 tggagccgaa cctattccga tcttcaacgc cggcatgtca tgccaagtta ggggtgccga
    55381 atactgccca atggacgaaa tggatgagtg ggttcaaaat gttctcgact tcatagagtt
    55441 cgctaacggg ccatccgatt ctttgtggga ttcgaagcgt tccgagctag gccatccaga
    55501 acccttcaat gtcaaataca taggcattgg aaacgagaac tggggaacgg agtatcacga
    55561 tcgcttcccg ttgttcagag aagctgtcaa ggagaaatat cccgaaatta ctattatttt
    55621 cagcggtcct ccttcatatg aaggagcttc gttcaatcgt gcatggagat gggcaagaag
    55681 caatggtatc gagatctttg atgagcatat gtatgcagtt ccggaatgga tgctaagaaa
    55741 tacagacaga tatgacaaat atgacagatc tggtccgaag gtgatgcttg gagagtacgc
    55801 agcccacgcg gccggcacca gaaacactct tcaagcagcg atagcagaag cagcactaat
    55861 gaccggcctc gagcggaatt ccgacatcgt aataatggcc gcttacgcac ctctgttcaa
    55921 cagagagggc tggtcacagt ggaccccgga cctcatctgg ttcgacaata ctcgagtata
    55981 cggaacaccc agttatcacg ttcagaaact gttcaacgat aacactggag atgtgattat
    56041 cccctcgtca ctgaccgatg aagacataga gataatcggt tactggttca agtcgctgta
    56101 tcatgtctgc agttacgatt atgaaagcaa agaactgatt ataaaaatcg tgaatccctg
    56161 gcccgaagaa aagaaactca gaattgagat cggcgaagag gttcagataa ccgggaaggc
    56221 agagatgatc tccattacct ccgagaacat ctttgacgaa aacacattcc agaatccaga
    56281 taaagtacga ccgaaaacgg tgggaatgtc cgacctatcc aacgaattta cactaacctt
    56341 ttccgggaat tcagtaacgg tattaagact ggagacgact ggtctctagc accatataaa
    56401 tacgagggag ggattaagat gcagacatca gtgaaagtgt ggccgagaaa gcttgggaag
    56461 ataaacgaaa tgatctatgg tcactttgcc gagcatcttg gaagatgtat ctacgatgga
    56521 atctataacg aaaaatctcc taagtccgat tcgcggggtt tcagaaaaga cattatggaa
    56581 gctataaaga gaatcaagtg cccaattctt agatggcccg gcggtaattt cgtgtctgcc
    56641 tatcactggg aaaacgggat cggccctctc gagaagcgtc ctcagctccc gaactacatt
    56701 tggggtggtg tggaatcaaa cagattcggt acagatgagt tcatacagta ctgcagagag
    56761 attggcaccg agccttacct ggcagtcagt ttgggaaccg ctactctcga cgaggcgatc
    56821 tcctggctag aatactgtaa tctggatact cctacgaagt actccagaat gaggagagaa
    56881 aacggtcacc cagaacctta cggcgtaaag tattggggaa tcgggaacga agtatacgga
    56941 ccgtggcagg tgggccattg cagtgcagct gaatacgctg agaaacttcg tcagtacacc
    57001 cagttcatga agggcgtaga tcgatcaata aaggtgatag cagtcggcgc cgacgaccca
    57061 aattgggata tgacggtctt gaaacatgcg gggcatctta tagattacat ctcgatccac
    57121 gaatatcacg ggtccgatga ttatcttgga acagtagcct ctgtctacta ttgcgaacag
    57181 agactgagac tactcgaagc actcataaag cacctgcgcc tcgatcatgt gaagatttct
    57241 tttgacgagt ggaatgtatg gtacaagata agtgccgaaa aggaaagcat ggataaggga
    57301 atcgttctcc ttgaagaacc ctatgccctg aaagacggcc tttttgcagc gggaatcttc
    57361 acggtcatgc acagaatggc cgataccgtc catatggcca accttgctca gatggtaaac
    57421 gctcttggca tgataaaaac gaagggagac gaaatggtcc tcactcctat ttaccatgct
    57481 ttcgatctct tcgttaatca caccggtaga actagactaa acatggcggt agactgcgat
    57541 acctacgaca tcaatacgag aaccttcgtt gagggacgaa acagcttcaa gctgagcggc
    57601 gtaccttatg ttgacgggtc ggcaacctat gacgaagaga aacaggttct aagcgtcgcg
    57661 ctgatcaact attaccagaa ggaagaggaa gtacgtctgg atctatcggg aatcgggatc
    57721 aagaagaagg cggttctgaa tgtattgacc ggttccgctc ccgatgagat gaacgatttt
    57781 gacaatccag atcgaataaa actaataaaa aaggaactag ataactgttc tacggagatg
    57841 ctccttaagc tcgatccgat gtcggcaaat gtaatcgagt tctcgctgga aagtagtctg
    57901 taagaccctg gaaggaaggc gaggcgttcg gttccggcga acctggattg gatgagatac
    57961 aatcttggtt aaggcatgaa aatgacgaaa tctagaaggt gatatgtatg tacttaatcg
    58021 gatgtgacat tggaactcag gggacgaaga gcgtactggt gaatgaaaga ggagaggtcc
    58081 tcgtagaagc tcaaagagag tacgacgtaa taaagccagg ttcaaactgg gccgagcagt
    58141 ggccggatgt ttgggcgaag gccgctttca acacgataag ggaagttgct gaaaaagcag
    58201 aagttgcaaa aaaagaaatc gccggattag ctatcagcgg cctttacggt ggatctggtg
    58261 ttcccgtcga tagagagatg aagccactgc atccatgctt aatctggatg gatcgacggg
    58321 caaccgctca gacaagctgg gtgaaggaaa acattcctaa agagaaaatc ttctcaataa
    58381 cgggaaacta tgtagattct tattacggct tcacaaaaat catgtggctc agagacaata
    58441 gaccggaagt ctggaaaaag acttatgaat ttgtgacgcc gaaagattat gtagtttatc
    58501 tgcttacggg tgaagacgcg atcgattatt cctctgcagg aaacattggc ggcgtcttcg
    58561 atataaggaa attgacgtgg tccagggaga tgtgcgaagc tctgggaata cctatgggac
    58621 tccttccaga aaaaatcgtg aagtctagcg atatcgttgg cagaataact gccgaaagct
    58681 cgaagttgtg tggtctgctc gaaggaactc cagtcgtttc aggaggcata gatgcgccgg
    58741 ttgcccagct ttccgcagga gcactcgaag aaggtgaaca cgtagcgatg gtagggactt
    58801 ctacgtgctg ggggacggtc cacgatggaa aggaaatgcc gtttggtctg gtcagttttc
    58861 cttatgtggt tcacgatacc gaaagaatct actcttttgg aggctcggca accacgggag
    58921 ctcttgcaaa atggttcaag gatgaattcg cagacagcga agagtctgtt ggaaaaagaa
    58981 ctggaacgtc tccttaccag ttgctggata gggaagttga aaagattcca gccggaagcg
    59041 atggattaat agtccttccg tatttcatgg gtgagcgttc tccgatttgg gatccttttg
    59101 ccaagggtgt cttctttgga gtgacgcttg tccacacaag agcgcatata tacaaggctc
    59161 tcatggaggg tgctgcatac gcactgaggc acaacatgga aaatggaatc aaggctggac
    59221 tgaaacttga cgatgaatgc tggatagtcg gcggagtggc caaatctacg gtgtggaaca
    59281 agatctttgc cgatattaca ggatataaga tgagacaggt ttccagtctc gttgaggcgc
    59341 ctttcggtga cgcattcctc gcaggccttg gagttgggct catagataaa cccgaaagaa
    59401 tcaaagactg ggtcaaattc agagatccga ttagccccga cccaaaccat agggagatct
    59461 acgagaaaca gtattctctt ttccgggagc tgtacgagaa aaccaaggag actatgtgga
    59521 aaatggtcta agcggtgaga atggatacca gcgataagag ttgagacggg aaacgatctt
    59581 ttgaggaaac ttgaagctcg aagcaagtag ttgaaagtca tcggccgttg cacagaggct
    59641 ttttagagag gtcgttatcg atctatcgct gagtggtctc cgtctggctg gtcgcagagc
    59701 ttcaaagata ctctggatcc gtttgttcgg aactgtcaag ttcttatgaa gcgcactaag
    59761 acattgaaga gtttaagacg gccgagttcg ttgaagccta ttgactctag gtaaggagac
    59821 tagcttcatg ttaggttgat ggggtttcac cgggcaacgt atcagtttat tatactgttt
    59881 cgccctatgc gattgagaag tcaaggtgtt caaaaacctc tctgaactgt ctaagagaag
    59941 tatgagcaga tgtgcatgag atttgttcgt ctcagcttct agaacactag aagaaatggg
    60001 aatcatgaat cccctagaca gattcgagaa cctggttcca aagccaggtg aaagagttat
    60061 gagacggttt gattgatttc ggaaacaagg tgttggctga gttaagaggt gcagaatcat
    60121 tcatgcttga cgaaactaaa tccataggtt acgacaaagc attgaaatct cgaaagccat
    60181 caatctgtct tggtctcaag cgctccaaaa atctaccgtt atttgtagtc ttaaagcttc
    60241 aatgtttttt ggataaacat gggtgaccaa tctctttgcg aaaaggagga ctgattatca
    60301 tcagcacaga tagctatgaa gtttggtttt tgacgggaag tcaagagcta tatggtaaag
    60361 aagttttaag aaaggtcgaa gagaactcta aaaagctagt gaacgctctc aatgagaaga
    60421 taactgcccc agcaaaaatc atgtggaaac ccgtgctaaa agatgcaatt cagataaaag
    60481 caacaattga tgaggcgaat tccaacaaca gatgcatcgg tttgatcgca tggatgcata
    60541 cgttttcacc ggcgaagatg tggatcgccg gtctgaaatc cgttcacaaa ccctttcttc
    60601 atctccatac ccagttcgat cagaagatac cgtgggatac catagatatg gattacatga
    60661 atacgaacca atctgctcac gggggaaggg agttcggatt catctccaca aggctagaaa
    60721 tgaagcgcaa ggtaattgcc gggcactgga cggacaagac tgtcatttca aagcttgaag
    60781 attggcttag ggcggctcta ggctggaacg aagcgcatag actcaaggtc gcaagattgg
    60841 gagacaatat gcgggacgtt gccgttaccg aaggaaacaa ggtagacgcc cagatcgact
    60901 tcggttacga aatccatggc tttgcggtcg gagattacat agaaggatac ttcaagaaag
    60961 tgaaaagaag tgaagccgag gaactagtta aggaatactt gaaagagtat cccgttgtga
    61021 aatcgacggt ctctgaagaa gaatttcagt cttcagttcg cgaagccgca aggatagaaa
    61081 tctctttgag aaggtttctg gaagatggtg gtttcaaggc gttcacaact acatttgaag
    61141 atcttcacgg aatgaaacaa cttccgggga ttgcagtcca aagacttatg agagacggtt
    61201 acggcttcgg aggagaggga gactggaaat cggctgccct tgtaagaatc atgaaggtaa
    61261 tggccgaagg catcgataaa ggaacatcat ttatcgaaga ctacgtatat cactttgaac
    61321 cgggaaacgt gaaagagtta ggggcacaca tgctcgaggt atgtgaatcg atagccgatg
    61381 gtactccttc tttagaagtt catcctctat cgataggagg aaaggaacct ccgacaaggc
    61441 tagtgttcaa tgcgaaagcg ggacccgctg ttcaggcctg tatcgtcgaa ttcggagata
    61501 gattcagaat gatcgtaaat gagctggatg ttgttaagca agaggtagcg atgcccaagc
    61561 tgcctgtagc aagggtccta tggattccga aaccagattt atcaactgcc gcaacagcct
    61621 ggattctggc cggtggggca catcattcaa gcatgagtta cacggtcacc acagaacagc
    61681 ttgaagatct ttgtgaaatg ttcggaattg aaatcgtcgt gatagatgaa aatacagaca
    61741 ttagggagtt caaaaaggaa cttagataca atgaaatata ctacaaactg aagaagcttt
    61801 gatcttagct ttgtcatgga aattgctcac gctgaatgtg aaaggaagag aatatgctaa
    61861 ataagcttaa ggaggaagtc tacaaggcta atatcgctat cgatgagtat ggactggtaa
    61921 aacttacctg gggaaatgcc agcgcaatat ctagagaaga aggtctagtt gttattaaac
    61981 ctagtggggt agactattcc atgctttcgc cggacgacat ggtggttgtc gatctcgaag
    62041 aaagagtagt ggaaggtcgt cttaaacctt cttcagatac accgactcac atctttctct
    62101 acagatcctt ccgtgaaata ggcggaatag ttcacaccca ttcaacttgg gcaacagctt
    62161 gggcgcaggc ttgtaagagt attccatgct ttggtacgac ccatgcagac cacttctaca
    62221 aagaagtgcc ctgcacgaga tttctgaaaa ctgaagaagt cgaaaacgat tacgaactaa
    62281 atacgggtaa gataatagga gagacctttt ccactagaga ttaccaaaga actcccggag
    62341 tactggtagc cggtcacgca ccttttacct ggggaaaaga tgtgaaggat gccgttaaga
    62401 acagcataat tctagaggaa attgcaaaga tggcatgcct gacgcttttg atcgaagggg
    62461 aaacggaagc gcttcctgag tatataatga acaaacacta tttccggaag cacggaaaag
    62521 atgcttatta cggacagaaa tagaactgaa gaaggcagtt ctactccaga aagaactgat
    62581 agaacgactc gataaacagg gctttaaact gcctctgttt gatgattcag taaagacaac
    62641 ctctggcagt gtcggaaatt tctcgagaac aggcgggagt tacaaaatgg aattactctc
    62701 ttgcccgggg tcggatcttc gtgttctttt gaccccgtgg attttatcaa gaagcagatg
    62761 tagacagtag ccgcttgaac cggcaatacc cagaaagatt gtctccgtat gattcattga
    62821 accgccaaac gactccagtc tgaaaaccac aacactttcg attaccagcc catatagaag
    62881 gcttccccat actgtatgaa aaaagcctct gtgactgaga aggtttgtca gtttggatac
    62941 aaccagtccg gaaataatgg caagtgaaaa ggaaagtgta actgccacag gcagtgaagg
    63001 aagcctggaa agcaggaatg gctgaaccct gggcgaaatt atggaaatgt atagagcaga
    63061 agcaacataa cttgcgatta gtatttcgat aattctcttt atcaacgcgc ttttcgaatc
    63121 tatgtcaggc agatcgcttc cgagtacata taaaagatat ccggaaccta tcagtctgct
    63181 atcctgaagt tgctgaacgg gcaagagttc aatagcactc agtgaaagta agatgaatat
    63241 cggataggaa gcgatacccc agcatatgtg tgtcttaaag tcagccatct tcctcactgc
    63301 cgaacttcgt cccccgactc gaaatatcga catagagaag cacatctttc agcgatatac
    63361 tcataccgca ttctacttcc tcgggaacgg gttcattcaa atcgaccgga tatagagatg
    63421 tccgattgca tagagagagg ttgactcttg agaaacgttt gtacaaggcc gttatttcct
    63481 cgtcctcgac attgagccca acggtcgccg ttatgcccga cataaagaga tactcaagat
    63541 catcgagatc aatcttcact aaatctgagc tgagtcctcc ctctgatgga gtacagaaaa
    63601 gaatgagctc tctcgaaaga ttgtaaaacg gtgtaccttc cagtctctcg tatgttctat
    63661 atttgtcgac agtagtttca tctgcaagaa gaacttgacc agtatattcg atcaagtctc
    63721 cggccttcaa cttccgaata ttctttatcg atatcggata gaacatctta agcgtttgcc
    63781 ttgccaaatc acaccaccta tttgctcagc ggaaaatcag tatgaatgaa tctcaacatg
    63841 ctttctatcg tttcagatac gaatttcgag tagtatttgt agaatgattc aagcctttcg
    63901 tatactggtt ttgagtctac tagatctatc tcgaaaacag ttgcaaccgg tctcgagata
    63961 ttcatgtcgg gagttacact cgagtttctg agaacgtcac actgttttgt tacctcgatc
    64021 ctgaacaacc ttgaaagctc cataattata gaacggtcga tgttttcatt tatctttggt
    64081 agaccaaagg cgatttttag aatgttttga aagggaaaaa ctaggacttc tccgtcgtcg
    64141 aactcaatcc actttctata attttctctg caaatagaaa acagcgtatc atcgataact
    64201 tccgacaacc tgaagtaatc ctttcctcgc agggagtcct tcagaatctc tttacgtaga
    64261 agcctttcat taccatagta agcaaggaag taaagcttca aaggactttg aaagatcgct
    64321 cgatggtaca tcagttccgt ttcgtcccca aagaaaccgg cgaatacttc catgtacggg
    64381 ttctcaactt cgtttgggaa ccggtacaaa gcgcttgctg tctcaaatgg agatgagttt
    64441 gaaagaccca cgtaacctct gccttcgctg tccacaaaga ccgaaagaac gggagcgttc
    64501 ccttttgaag tttgaactac cagtgtcatg aagtggccaa aagcattgtc cagtcgatca
    64561 gagtggtctt tgacaagttt tgcccacgag acgaagctgt cttcaacttt ttcgtagact
    64621 ctcctgagaa tttccctgta gctttcgggg ccggaatagt aagtcactta agaaacacca
    64681 ccgacgaacg tccgtatatt gattttgctt cttcagcaaa ttctgaattg tggctttttg
    64741 gtagaacgaa caatactctt ctcgaaactg taggcaaatc cgtgaacccg tcggtgaaga
    64801 ggaggagacc ttcaggtctc agcttttcct gagcgtaatc gacggccggc tgaagatcag
    64861 tctctcctct tcccatgagc ttaaggtctc tccacatacc ctttccatat ttcatcacac
    64921 tctgaaccgt ctcatcaacc tgcacaagcc agaccctgga atctgtcact cttgtaactt
    64981 cgtctatctc actgaagaaa gagttgaact cctcttcgat aatgctccca ctggtatcga
    65041 caactaccac cagtttcgcc gcatagtcgt ttctccatcc cggttgatca tcatatcttc
    65101 ggttgggcct catcggagtt ctgtaccttc caacctgcat ggaggaacca aagaaacgtc
    65161 gaatcatatt tctccagtcc agaacgggat tgtcgagaac cagcttcata actccctcga
    65221 gaccttctgg catgccggtc ttggcctttt ccatagtctc agagatcacc tgcttcagta
    65281 gatcctctac aaactccttg ggaagctcga atttgccaaa gtcctcatgt gaatccggcc
    65341 tggagatgtt tgaattgagt agttcgagat caatggtctt gttcttcttc atgaagtcaa
    65401 gtccccagtc gtgatagaac tctgccgtca ttcccggata ctgcattgga ggtccaacga
    65461 aaaacctctc gttgtcggtc ccgcagcctt ctttcaaaat cgaatccatt gggaggctga
    65521 acgcatcgag ttcccgtatg aactgattca cggccgcgtc catcgacaga tcccacagcg
    65581 ttttttctct ggacttgctt accggtatca gtatgtgacc atttactatg tggagacact
    65641 catgctttag taatgccttt gtaaacgtca aaggtctgtt tcgaagagta tctggattgt
    65701 ataggagctg aaaccttcct cttgaactaa ctcggagttt caacgttctt accgcaaccg
    65761 aggggattct atctatgaag aggagcagga aattgtagaa ggggctgtct tttcccagtt
    65821 caaagactgc ccgttccatc aattcctcat ttttcatcct tttcgctcct ttgaaggaat
    65881 ttcagaacgt cgtgatccat tgccagttcc tcaagaagat tctcataaaa agcccgcttc
    65941 agtcccttct ctccctctac ctgatgaaca atgtgcctta tcaccgagaa gaatgagtct
    66001 tttggaatca ctttcccaat tctgagaaga ttaccggcga ttaccggaac gttctgaaag
    66061 tattcctgaa cttgcaaatc atctagctca gaaagatgct tcgtaacacg gagcaccgag
    66121 gcactggtcc atgagatgtc cccctccgaa agcctcccta aaatgttctc gtcaagatcg
    66181 agaaggagag ctcttggaga agggagaagt tcgctacctt gaatcttgct catgaaagtc
    66241 ctggccgcct cgggaccaac aatgcctgca gctattacat aaccgtatct ctcgatctct
    66301 tcgcttgaaa ggtcagcgaa tactcttccg agcttatacc agctacgagg gctcggcctc
    66361 agctcgagac gcatcgaaac tattgtatcc ctcgaaagaa actctggata ctccctaata
    66421 aaattcgtca cgttgctcgg catcccctga atttcagccc atttaagcca ctcatcggga
    66481 tctggagtga gctctatgtg gaaaaacctt gacataaagg cgggatcagt tattagctct
    66541 acctgatcat attcctcatc cggcgggtta gcagctgcca ttatccagct tccttccgga
    66601 agaaagtgat tgtgtattct tctatctatc aagagctgca ttattgcatt tcttatcgat
    66661 ctgtgagctc tgttcacttc atctatcatc aatatgacat ttccttcggt tggccaccag
    66721 tcaggcctta agaacagtgt ccgctctcca tcccttgaag gcattccgat gagatcaccg
    66781 ggctccatct gtgaaatcac tagaattatc agttctctgc ccgtctcgga tgcgatctcc
    66841 ctagcgagat ctgtctttcc aaccccaaag tggccccaga gaagcgggat ctctccagac
    66901 tccataatta ttttactcaa gtgttttgca tcttgaacct tcaagcgtat ccctccaaaa
    66961 tcctcattca gacaaaatta tagcacagtg atctgaacga tgcccaggct atagagaccc
    67021 agagagaaac cggcaaaacg agtcctaaga gccaggtaaa aggtgaaact atagggatat
    67081 tcatgaaatt aagaaaaaga ccgatatttc cggaaatata cagggtcatt ggaagcgttg
    67141 aaaaaacagg gagaagtgtt gatagaagga aggatagaat cgcacccgaa agacttagtg
    67201 ccttaaaaac gttcttcgaa tccttcgttc caaagctctt ctcgagaaat ccccctaaca
    67261 atgaaagaac gaagatcgta ggaatcataa atagaatgaa attcatcatg aaagccctgt
    67321 actgtgaagt gttgttggag acgtacagac ctacaagcaa aatgacgaaa gaatacagag
    67381 aggtgataac gaacgatttc gataagatcg tcttgccccg cctgatggga aacaaccacg
    67441 aagtatcgcc aagttgcctc tcaaaagcaa aaagtgcggt cgaagagaca gttgtgtagt
    67501 tactgaagag gaaaactata agacccaaca gctgtaaaat accaaagctg cttagaagac
    67561 ccgggttgac aagaacaacc acagggacga acagtgaagg atacagaatt gcaaacatta
    67621 gtttgggctc tcgaagaagc aacaatgaat cctttatcag cagacccaaa aacaatccat
    67681 gaactttgaa atctccattt gatttcgtgg acacattccc gattggtcta gtcataagct
    67741 tctggagaac cttgctgtaa aggaagattt ctagcagtgc tagagaagaa aagataccta
    67801 tcgaggatac tatgaacacg gcaagatccc ttgtatctcc cgtaattgct cttacaaagg
    67861 gcgaccaggg aagatagggg gaagtgacaa cgctctgaag cccgccgaat tgttctgcaa
    67921 cctgatcagg ttttgctaga tcaattgcgg aaaagtccat gaatctcaat gtgaaaatga
    67981 aaacgaaaag cgtaaccatg gtcatcatag ccgaaagcgt cttaagaaag cgccccttgg
    68041 atagaaagac aatcacgaat gagattatcg cagttatcgc cagagtgttg agcacgaaga
    68101 caaagagcaa gacgagggcc atccagaatc tcccgtccga gtaccccaag cctcttaatg
    68161 cggccatgaa gaccggcgca aaaagaagta tgggcaatcc tcccgcagcc agaacttcaa
    68221 gcaactgaac gtttgcaata gtggctgtcc ttaccggcaa attcagcagc atttcaaccc
    68281 gttcagaaaa gaaaaggttt gagactacta ttggaaggtc gctcccaagg agtattgaga
    68341 atattatcgc cgaaaagaat gaaatgacta tcagttttag atcgattccc gtcagctccc
    68401 ttaactgatc ggaaagatcg gcgaccctgg agaaattcgg cacgaggtaa cctctcaagt
    68461 agaaggcaat cggtacagcg atcattatca cgaatgcgac agcaagaaaa gaagcaacaa
    68521 acgctctctt tccctgacgg gtcagcattc tcaacccttc gacgcctcgc cacttaagaa
    68581 tcaacaagaa atctctcatg aaactatttt gccacactct acaagtcttt agccaaaagg
    68641 ccttctgcga aatttacgaa cctctttcaa tgaaagccag ggtcgaagtt gtaaaagctt
    68701 cgtcacgaca aatcttcatt aactcagaaa atttcttttt cagtcgacaa tacccggtta
    68761 tgcatcgtgg cgcagaaaga agtgccttca tccaggcaga ttaccaataa cagtgcaata
    68821 tcatttagga agcgtgagac atttaaacag ccagctaaac ggtccatcgt tcttttgaga
    68881 aggagcgagt acaaacgcaa acagtttcct cccggaataa aagtatccaa aagagctttc
    68941 ggaaaagact ggagaatacc aataaccagt caccactgaa ccactgtttt gaaccctaag
    69001 ttcccgaaaa gtctaacgat gaaaaccttg taaagggttg aaaagactga gttagggttg
    69061 ttttctctag gtttatgcct cgttatatat tataatgtat gtaacgaggt gaagagatgg
    69121 ggattgtcta tcagaagaac aagaaaactg gaatcacata tgtctacaaa aacgaagcct
    69181 attgggacaa ggaaaaacaa cagtccagag ccaagagaac cttaatcggc aagctggatc
    69241 caaacactgg agaaatccta ccgaccaggt catacaggaa ggatactgaa aagaaggata
    69301 gcatttcgaa aaagcccggt tcagttccca tcaccaaaat ccgcagggat ttctatggtg
    69361 ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    69421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaaaagc ccggttcagt
    69481 tcccatcacc aaaatccgca gggatttcta tggtgcttgc tatcttctcg atcaggttgg
    69541 aaagatcacc gaagttgagg cagatttgaa agcctgtttc cccgatcgtt atagaatgat
    69601 tctgtctatt gcctattatc ttattctcga agagaacaac tccctcagta ggttttcaca
    69661 ttggcagaga ttgcatgttc atccttatgg agaagacata ccatcacagc gcagcagtga
    69721 gctgttccaa tctatttccg aagaagagag gatgatgttt ttcaagaagc agggcagacg
    69781 tagaattgag aaagaatact gggcctttga catcacttcc atctccagtt attcagagac
    69841 actgagacag gtaaagaaag gaagaaacaa agagtacgag agactacctc aaatcaacct
    69901 cgcccttctc ttcggtgaac agtctggact tcccttttac taccggaaac tccctggcaa
    69961 tatcaccgac gtgaaaacag tcaagcaact tatggctgaa ttcaatgtca tgggatacaa
    70021 aaaggtaagt gtccttcttg acagaggctt ttatagcaga gagaacatca acctgctctt
    70081 caagaaccac cagaaattca tcatcggtgt caaactgaat ttgaattatg taaaagagac
    70141 cctggaagaa gagcgtgaaa acctccaact ctggtcaaac ctgcaacccc agtttggagt
    70201 atatggcttg tgccggacga tccagtggga atatgaacag gaaagaccca ataagggaga
    70261 tgttctcagc tcgaaaagac gggcatatct tcatcttttc tacaatggtg agaaggcagc
    70321 caaagatcag gcagagatga acgattatct gaccagacta tacaatgatc tggtaaacaa
    70381 cacacgcgaa gaatcccgca tgaaggacta tgatagattc ttcactgtta ccagtacacc
    70441 aaaaaggggc aggaaagtca caccgaagga agaggcgatg cgtgaagcgg caagaaacta
    70501 tggctatttc gcactgctct ccaatgaagt caacgatccc ttcgaggcct tatctctcta
    70561 tcgcagtaaa gacatagtgg aaaaggggtt tgggaacctc aaggatcgtt tgaatttccg
    70621 tagaatgcag gtttcctcag aactatcact gaatggaaag ctgtttgtcg agtttgtcgc
    70681 cttgatttat ctttcctaca taaagaagaa gatgcaggac accggtctct tcgataactg
    70741 gacattgcag ggtctcctcg acgaactgga caccattgaa aggtttgagt atcccgaaca
    70801 tggccgcttg atcggagaag tcactaa
//
LOCUS       DAOQYI010000006        68762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_423_length_68762_cov_75.300770, whole genome shotgun sequence.
ACCESSION   DAOQYI010000006 DAOQYI010000000
VERSION     DAOQYI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 68762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68762
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_423_length_68762_cov_75.300770"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKL93_03435"
     CDS             <1..540
                     /locus_tag="PKL93_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ70347.1"
                     /translation="GELATRNGLLALPLDEIKMRVERFGRFVRRSEAENDESMRQIIP
                     YAVFRNRDEYLLMKRTKKQGEARLHELYSIGVGGHINPEDGRLPWEAFENGFQREIRE
                     EVSVKIHSMDYLGILNDLQTEVSRVHIGVVYIAEVDFGGFNEKEKFTGAMAEIKVLSE
                     YRESMETWSQIVLDYLLLN"
     gene            501..1370
                     /gene="ispE"
                     /locus_tag="PKL93_03440"
     CDS             501..1370
                     /gene="ispE"
                     /locus_tag="PKL93_03440"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNQ70348.1"
                     /translation="MVSNSSRLSSVKLRCPAKINLYLAVGKSRSDGFHEISTVFQTID
                     FYDDLFVKHDTRKLYFKCNTSLRWDKENTLYKVIEQIESEIDRRIEIGLELKKRIPSG
                     AGLGGGSSDAAALLKYLAGLFNIDEERVSRIASNVGSDVPFFLKGGTAIASGRGEILS
                     FPGDVSGYSVELSFPGIEVSTPLAYEFIDEDNPELHLDCEMAEKYYLALKSHDIEAVR
                     RMSYNTFEKSIFDRFPMIRDCHSMLSNKNGGAIKTMMTGSGSAIFSLFEGCKGRYSFV
                     TSEELNEKWCSMV"
     gene            1352..3040
                     /locus_tag="PKL93_03445"
     CDS             1352..3040
                     /locus_tag="PKL93_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNQ70349.1"
                     /translation="MVFDGLILQRVLAEISDVSGQQLRQVYQIGRSEFFFKFSKRGIE
                     ISLNPSSPYIIESNRNQNSPSIETPFSLFLRRHINGFFLTKIAQLDMDRITRMDFEGR
                     DAFGERNKYSVIAEFIGPGSNIMVLDEEERILQVFREMISSKRTLARNVKYYPPDSPG
                     KSLRNLSKENITLTLLHSGDTLSRAIMKSFTGFSRATAENIVGFLQIEDVVPSSLREE
                     RLDEVAEFLMSLSLDTNGRSLFVIDGTKGAEISPIPLDHKGRCEKVGASEAIRRAIKS
                     SGAETEVDRRKQSIIRKIDKSFKRLFKLIDKLETELSEVENYRDFKKYGELITANLYR
                     LRERRETVELEDWETGETVEISLDSRLTPSENAQLFFKYYSKSLRKEIQLKKRLRVLR
                     DEVNYLDQLKEMVSQAESLEDVREFSSELEEAGIRRKEKIDKKRKKRSQKSGPRVFER
                     NGFKFLVGRNNIENDEITKNASRDDIWFHARGIPGAHVILKKAGKEITPEAILYGSLL
                     AAKYSRGRQSGKVEVAHTEVKNVKKPKGAKPGMVTYRKFESITVDLTKEMVGRN"
     gene            3037..3957
                     /locus_tag="PKL93_03450"
     CDS             3037..3957
                     /locus_tag="PKL93_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70350.1"
                     /translation="MIRLPPYIFFVGGFLTYASIFFSSATVSMTMSVIGMTISLYIWY
                     ILAWNRDRHLATMKKKGYVKPQNCAEQKIAGNSRIWVILYSASYLTMNFAGLYIIKAI
                     IENIDMSLDTMSMEELITLLGSGYVLSSWLFFITGIASIFLYGKLITMLYNDEMKIQS
                     LESKQRNIPVLILKPLSVVIMVIFTLITYGLFSWFMRYRLAAIQRFHNQIEKKLDELE
                     ESFKDKAKNIKKEKEEQPSKSISEEILEKYSRSLASSEESDDRKLIIASLFKDLGNLK
                     SDQARSLLNDLLSKQLLTENEFNRLTRLLV"
     gene            3972..4820
                     /locus_tag="PKL93_03455"
     CDS             3972..4820
                     /locus_tag="PKL93_03455"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNQ70351.1"
                     /translation="MIRFRSATITLIIINVIVYLLVAFMGLFRGPQGISYRDLITVYG
                     GVSRFALSNGLIYTPLTALFLHGNLMHILFNMWALFQLGHVVEGVYGSKWYLLFYFVT
                     GISGSLSAAAFSNAFTIGSSSAIFGLVGILFTLGLKKDTPVVLRSITGYSLLPIILIN
                     LFLGFSIPGISNAAHIGGLVVGAIIGWFAKPAYARSARSRRVYTRVKEKTPEEASRDI
                     LVKYIPVLNGMKDNRSEERTIRIAQLRSELSSLKDQEIGSKVLWELYKRDLLSQEEFE
                     RLRRFL"
     gene            complement(4851..6014)
                     /locus_tag="PKL93_03460"
     CDS             complement(4851..6014)
                     /locus_tag="PKL93_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNQ70352.1"
                     /translation="MHITPEVRKAAREIKTPFLIMDMDYVRNNYFDIVSHVRNVQVFY
                     AVKANSHPRIIETLRDLGSNFDVASRGEIEKLLALGISPERMSFGNTIKKVEDIAYAY
                     SVGIDYYAVDSEMEVEKIAAHAPGSKVYGRIATSGGDCDWPLSRKFGTDVGHVISIME
                     YADQLGLDAYGVSFHVGSQNYNVNSWDDAIRDASEVFKTLRAKNINLRMLNLGGGMPV
                     KHVREIKSVKSYGDIINRALDKYMSFVPDLELFIEPGRSMVGNSAILVSQVILRSKKG
                     SEDWVYIDAGVFHGLTETIEGFRYEVLTEGKVDDTKISFHLAGPTCDSVDTIYHEIDL
                     PKNIGYGDILYFINAGAYTTEYATNFNGIDAPRVLFVEDFIDAEMPIERDFME"
     gene            complement(6141..7091)
                     /locus_tag="PKL93_03465"
     CDS             complement(6141..7091)
                     /locus_tag="PKL93_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNQ70353.1"
                     /translation="MRSIFRDFTYDYFGILSYPKERWFDFWTSYREKHPRVLEEYMFK
                     NNLDEEELSLSLDKLERREIDRLSHYWEIQGPIEKSRVLKELGKMSPQLHLEREDFVI
                     HILGALGRQQHLIVPTSRGNVVMIDLLFCWKEGSIKDFSSVILAALKDFLEYSRVNVR
                     HTMDSGKRSERFDLILDVIEREIKGRSFKEKMAMISKLLDKYVDYYNWTGFYLVNEES
                     SLVLGPYVGEPTEHVKIAFGSGICGQAAETKKVFLVPDVSKENNYLSCSSKTKAEIVL
                     PLIVDGKVIGELDIDSHLQNSFDRLDEEFLEKVCGLLIAS"
     gene            complement(7095..8261)
                     /locus_tag="PKL93_03470"
     CDS             complement(7095..8261)
                     /locus_tag="PKL93_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNQ70354.1"
                     /translation="MLQVFLFFFLTFLIFRDAFYSFSAKDLAAESFLVVGVDSGGNSS
                     DAVGGRTDYISIVYFRTSGGLLLKSIPRDTVVTYRSEKRKINSLYNSFGIEALIEEVE
                     KLTGRTITGSVVVDFNTVTEITTFTGPIRVEVTTPMHHDDFQQGLHIHFEPGIYFLEG
                     EDLLKFLRYRSSDSGDLGRIDRQKRVLEQLIRNLVSAGPSKMIEMIDFVLEKTDISID
                     KKTLTDFAVGFFTGKRSVSFTQIDYYIDDEGQIVPTGPGSDEPKAVEIESRSPKILVI
                     NNIPDYEERFGDFAETIKSQWLAQAGVKVDATGFVPNISGIEKRDTYLFINSKDSEIR
                     ELFSKAHVYHRPFVMLTSSFGGLDYYYALIDSLSKNRFYPAKYDAYVLLGVGGR"
     gene            complement(8297..9007)
                     /locus_tag="PKL93_03475"
     CDS             complement(8297..9007)
                     /locus_tag="PKL93_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70355.1"
                     /translation="MSLIKPKRRRGPKLLWLILVIVLVVVAGAGYVYFIVNGVNSSDE
                     MKAEYVDYLFYWQSADNPLYYYVRTTTVGRTSLVIFPIFATIQNSKEVLDPQLGADAV
                     EAVQSWLGTSGDFSYFVNFTPDLIDALSSKLGVNASNPVELIDAMALRGFKLFDYWKI
                     NDFVQTVKEYDVASILTSEGIAVLLRRLGNSSRMTYKLETLTEFPMKISVGVGGETVS
                     RMYLKPDSLETVKKALAD"
     gene            complement(8994..9587)
                     /gene="yqeK"
                     /locus_tag="PKL93_03480"
     CDS             complement(8994..9587)
                     /gene="yqeK"
                     /locus_tag="PKL93_03480"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HNQ70356.1"
                     /translation="MITEYDTVTREVRAKAKSMCSPERFEHILRVERLAKRLSSIHGI
                     DSSGVGLAAVSHDMFRDQAEDELMRLAISFGIDMTEMERVAPVLLHGKVAALYLKEEY
                     GVDGDVFQAVYWHVSGIPGMSTTGKILMISDIAEEGRDFSEALHIRETAEVDLEKTFV
                     DVIRLKITWAVESDSLLLPETVWTWNELHGGASHVFN"
     gene            complement(9559..10782)
                     /locus_tag="PKL93_03485"
     CDS             complement(9559..10782)
                     /locus_tag="PKL93_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70357.1"
                     /translation="MKKILVSLMLVFLASISISQQLYIFSGFSIYSGEVVFENGTAII
                     ELPYGADIVPESLSVFGAPERTHIRYVPADSLKSLYEKHIGRWIKFLFDDGRSEMLEV
                     VSDFPVFRNSYGEILVNPKGSPVFTGEYTPAGRFLLETQEDYSGHARFAYQTTNNNWT
                     ARYSLSVDDFSMTGLMIVDLGVEPPSTITLVSSNPEFGYSARESSSKMANASYSISPT
                     TSGAETRLYEISVPLSRGINYISFLSRFVEGEKRLVFRANWGASNYTGVDIDVRLKEV
                     PLDLPSGIIDIWSDSVYLGSAGIGNIAEGENILLESVAKSIEIAGKKISETMKTDGTY
                     RYIRNTYYVRNLSEETSAVVIEDYLGQNVDQINMENNEKFEYVKAEGKWSAVVTVEPD
                     QIAEVKVDYRVRYGN"
     gene            complement(10799..11077)
                     /locus_tag="PKL93_03490"
     CDS             complement(10799..11077)
                     /locus_tag="PKL93_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNQ70358.1"
                     /translation="MHVGYMTYLLRLYGISSLKEKRAVIKPVLNDLRRKFNASVVETG
                     KHDSKQESEITVCMVANERGELDSLFQSVWQRIIWNGLEVSGEDGEIW"
     gene            complement(11181..13253)
                     /gene="fusA"
                     /locus_tag="PKL93_03495"
     CDS             complement(11181..13253)
                     /gene="fusA"
                     /locus_tag="PKL93_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ70359.1"
                     /translation="MDKIPVDAKRDIVLIGHHGSGKTQIVDAMLFNAKLIDRIGILAT
                     DSEEVEKEKKASFSMGVTSLPHNDKRIYVIDTPGMSDFYAETANGIFASENVVAVINS
                     TAGLEIQTERFGSIAKELKKGIIAFFNMLDKERAGYEETLSDLADTFERTPVLVQLPI
                     GREADFRGIVDLVKMKAYIYENEGAFKEEDIPADLKSAAEESRTKMIEDIVQNDEELM
                     MKYLEGEELSTEELITALRKAYLANEVIPVLLGSAGKNIGISQLLDFVSAVGKGPSEA
                     SPKAATLLSGEKIDVVPAEEEPLVAYIFKSVVDPFVGKLTFMKLLSGTLKQGDSFYVV
                     DQDSSEKVGHVMLPEGTKEIEVDEATVGDIVKLSKLKKSAAGNTVAHKDRQLKLDLPV
                     MPEPMISKSIQPKSKGDIDKISGGLARLAESDPTFKWENDPETNETVISGLGSVHLDI
                     MIERLKKLFSVDVEVGKPKIAYRETVRKTVEAEYKHKKQTGGHGQYGHVQIKIEPNER
                     GAGFEFIDKIVGGVVPKNYIPAVEKGIVEAMKKGVLASYPVVDAKVTLFYGSYHDVDS
                     SDMSFQIAARQAFKNGMAEANPVILEPLMDVEVFVPEESTGDIMGEITSRRGRPMGME
                     PQGKGTSKVVAQVPLAEMLDFANKLSSITSGRGYFTMKFSGYQETPPDVQQKIILERQ
                     RELEEQQK"
     gene            complement(13282..14955)
                     /locus_tag="PKL93_03500"
     CDS             complement(13282..14955)
                     /locus_tag="PKL93_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNQ70360.1"
                     /translation="MKRIIFICFLLISLVSSAKNLIYLDERGQTRVFTNSVVTDRSMD
                     FVKLDIIGELIRGVVVSQSLRQEETIMILPSGIIVSLSHETKRATVEFGSEFDNSMIF
                     KDGMVYINAELLAAITDKSFFSETEALILYAQPVTIKSIVNRPESVSITLDRDVLPDF
                     FTVWWTGSGSLALTLKPAKIDPFLDAEGVTVATGRGFVKISVEKPWPDVEYEIKGRTL
                     TLMGKSGIGRTIEQETSTDFSLNIFESYSGGQKFTMSYLEMNGSSFSFGVQLADNGIG
                     GLEKATDVLRESGSEIMINGGYFDQNQNLPIGLLVQNGKVLGLPSLGRPAIYFTEDGK
                     TYVTRMDILYLAKFDDRFVQITGVNSPYRGEAVLYTDEYRNSIPEFDDFTYLVVKDGR
                     IVKEGFSSKVERDTDLLVLSPSSLQKIGDTAFVGRSVSFELMNSFGVKITGAVEGGPM
                     IIHNGRPVTDYEQNYYSASLLDVRAPRTLVGIKETGGVVFIVIDGYQHSSYGLTFKEM
                     IEFFKDKGFESLMCLDGGKSSVMSVNGEIINSPSSGVPSLPVIITGSRK"
     gene            complement(14968..16269)
                     /locus_tag="PKL93_03505"
     CDS             complement(14968..16269)
                     /locus_tag="PKL93_03505"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNQ70361.1"
                     /translation="MRIYDIIMKKRNGFPNTREELDSLIMGFVEGIVPDYQMAAWLMA
                     VYFNHLNAEERFHLTEIMIESGDRIDLSSIKGRKIDKHSTGGVGDKVTLVVGPIVAAA
                     GLVFAKLSGRGLGHTGGTIDKLESIPGFVTSLDIEQFKRQAERIGIALAGQTAQVAVA
                     DKKLYALRDLTATVDEISLIASSIMSKKLAVDSDGILLDVKIGTGAFMKDLDGAKTLA
                     RAMIDLGRKNGRATKAVISDMNQPLGVSVGNSLEVVEAIDTLKGRGPQDFSSLCRIIA
                     GQMLVAGGMKSEADALRTVDRLILSGEALRKFDEFVYAQGGPEGFSASYAVHFKEAEI
                     VEEVRTSVSGYVQSVDAESIGLICMRLGAGRERKEDVIDPSVGVRILKRIGDRVEKDE
                     VIAVIYANDKRNADNEAKKLVDSFVISEQKTSPPPIVYEVI"
     gene            complement(16266..17516)
                     /locus_tag="PKL93_03510"
     CDS             complement(16266..17516)
                     /locus_tag="PKL93_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ70362.1"
                     /translation="MLYAVLAAVAVIAFLFMILFRHENQKNKRLEAAKRRFADLVEVP
                     RSAEFIFIYKKVQQLISSMKEEIALLDTRIENLVMIFDNLSDAFLIASEDGKIEYANK
                     AAQELSSKQLVGLRISEGIDSYYIGDLFEDSVRTQESQESEITIYYPRRSVRNCKIMR
                     VTLKSEVRYLILLRDITKEKEVEAMRRDFVANVSHELRTPLTSIHGYAETLAEDDLED
                     KETVYRFLSIIENESARMTRLINDLLDLEKLESGEASFSKEDVELGEVVNYVMRIVEP
                     LASEKNVSINVDVDEGIFVEGDFDRLVQLLLNLVDNAVKYTFAKEHGPKEIWLRAYAQ
                     NNSAMIEVEDTGVGIPEDSLKHIFERFYRVDKARSRKMGGTGLGLAITRFIVEKHGGT
                     ISLESEYGTGTILKVKLPLKKVFE"
     gene            complement(17521..18240)
                     /locus_tag="PKL93_03515"
     CDS             complement(17521..18240)
                     /locus_tag="PKL93_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ70363.1"
                     /translation="MAKKSILVVDDEPSIVELLTFNLKKEGYEVLKAYDAEEALKIAE
                     DNETDMFIVDIMLPGMDGFELVRNLRATEKFRQTPVIFLSAKSEEFDKVLGLELGADD
                     YITKPFSVREVLARIRAVFRRIQQSAQAKEERPKKILARDLEIDTEKYEVRVRNRMVN
                     LTPLEFELLRFLAENEGKVFSRDVLLDKLWGYDYYGDTRTVDVHIRRLRTKIEEDAST
                     PKYIITVRGKGYKFRDPGKED"
     gene            complement(18212..19687)
                     /locus_tag="PKL93_03520"
     CDS             complement(18212..19687)
                     /locus_tag="PKL93_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ70364.1"
                     /translation="MRKPRDRKSWSEYEIVSRLRSRERFVKEIRGFGDVKVAIIYPNS
                     YKVGSASLSTHVLMTKFNELGSVRAERFFFHEGFSKYYSFDSLTPLDEFVIWSFSVHF
                     ELDLLNVFEILNKFSIPLRKEKRNRFHPLILIGGALTYFNDSLVSEIADVVYRGDLGE
                     KFLEQLSAIDHRMSKEEMVSTLETEHQETIFSNCLGESAFISPDSVFGDRYLIEIGRG
                     CFRKCKFCVAGYKFGRPRFRELKDTIDLMDSVAGFTKRFGLIAATVTDYPDIDEIAEH
                     CIEKGYELSVSSLRLDSLSDTLMKSLKLSGQSSFTIAPEGGSQKIRNAFAKGISEEDI
                     TEALELGRNNDFHRVKMYYIYGAVFETQDDRTEISKTVHQALEMGYSNVVLSLNPLIP
                     KPGTPFENMPMESVGVLRKLEREIRSELMMEGVKLDFESLKESRLQFALASIDREKGR
                     ELLELVERGTDPTPILNKYAEDVNSERKEWKEHGKEEYSGR"
     gene            complement(19680..20087)
                     /locus_tag="PKL93_03525"
     CDS             complement(19680..20087)
                     /locus_tag="PKL93_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70365.1"
                     /translation="MLDLERLRSLENSEFTQTVIPTDVSLRWSERYDVELLNLLSTSG
                     LMKIVHSCGHNLISDFESDSLVSVVSETSVKHYSPILMEEELVINIRIVSVSDSAEVH
                     FSGSVVQLGTEAGAFEFSRKFISVDFFRRAFGA"
     gene            complement(20100..22001)
                     /gene="ftsH"
                     /locus_tag="PKL93_03530"
     CDS             complement(20100..22001)
                     /gene="ftsH"
                     /locus_tag="PKL93_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNQ70366.1"
                     /translation="MDQKPRIGMIVFYVVLGLFLLIALRGLYPTETSIVVPYTKFLDD
                     FNNIIDIVVYDSGKVVYITGDNPRRSLETYVPSQTLVTQTFQNHIDLLAERGANITFE
                     RGSDSLFWVNLLGTLIPLGIIIFIWFFLMRSMSGRNSQAFTFTKSPAKKYVNTDKRVT
                     FKDVAGVDEAQQELADIVSYLKDPSIFADTGARMPKGLLLVGPPGTGKTLLARAVAGE
                     AGVPFFFISGSDFVELFVGVGAARVRDLFTQAKSSAPSILFIDEIDAVGRHRGAGLGG
                     GHDEREQTLNQILVEMDGFDAKTGVIVIAATNRPDILDKALLRPGRFDKKISVDPPDL
                     KGRSEILKIHMRGKPIDPEVDVQLLARRTPGFVGADLENLINEAAILSARKKKKMIGM
                     AELEEAIDRVLAGPAKRSRIINDREKKILAYHELGHAVVGLILPKAFPVHKVTIIPRG
                     TSTLGFTESLPLEDRYLITKSELLDNMAQALGGRAAEDLVFGEITTGAASDLERASAM
                     ARSMVTQFGMSERLGPIAWGKEEEEVFLGRELTRMKNYSEEIASEIDSEVKTIVLTCY
                     EKAKRVLSDHRKKLDDAAEYLLQKETISGKELADILGLEGGNYYKDDLETIDIAPGAS
                     AEEPDIAKD"
     gene            complement(22006..23397)
                     /gene="tilS"
                     /locus_tag="PKL93_03535"
     CDS             complement(22006..23397)
                     /gene="tilS"
                     /locus_tag="PKL93_03535"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNQ70367.1"
                     /translation="MDNFELEVLRFVKEWDLVREGQRILVAVSGGKDSMALLNVLNNL
                     KSTLKIELFAANLNHGLREEGPQEEARMIEQFCEDRFIDFFHEKRDVREYMRTKKGES
                     TESAAREVRYGFLRDVKSMIGADRIALGHNRDDLVENTLLRLVKGTGMKGVIGLRPSN
                     GDLIRPLLFSDMQRIIDYVTINKVTFMEDETNAEDGFERNFLRLRVIPELRKINPALN
                     KAIWRFFENIYDGYSLIDSRVETLMSRFGKADGIYFAKLSELREIEKPLILELVRKIV
                     SMMSFDNYPPSRQRALAFFDLFSKDEGRWTVEFKENLKASRIGKYVFFYRGTLEFHGH
                     EKSFIESIPFVCEAEGWQIKVESKAGISSENYGEFDGAFKSICSDSRIIFPLSIRGMN
                     NKDKIIPFGMKREKKVVDILSDKGLKGFSSMIMVLENAFGEILWIPGVVTSELCRVGP
                     SSRNNVVFSLERR"
     gene            complement(23440..23515)
                     /locus_tag="PKL93_03540"
     tRNA            complement(23440..23515)
                     /locus_tag="PKL93_03540"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23480..23482),aa:Thr,seq:cgt)
     gene            complement(23560..25092)
                     /gene="rny"
                     /locus_tag="PKL93_03545"
     CDS             complement(23560..25092)
                     /gene="rny"
                     /locus_tag="PKL93_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNQ70368.1"
                     /translation="MFLIGIIAGLAAGIALAYFIFTPSAVKKAKEELQLQLKSAKQDS
                     ESIIKRAQEEADHLKKKALIEGREELHRLREEQEKDFKRDREELKQWEDRISRREDNI
                     DKKEEAIEKTRMQLDLERARVEEMKEEAEKKLFSLAELSMEEARNMVLKRAEEIYEHD
                     LAQKLKELKDHYDEEGNRYAKWVIVNSVQRYAADYTGDVTVSAVSLPSDEMKGRIIGR
                     EGRNIRAFEKLTGSDLVIDDTPEVVVVSCFNPLRRAIAKMTLDRLVEDGRIHPARIEE
                     MYEKSKKEIYNEIKEAGQEALMKVGIPSMHPELVKLLGRLKFRTSYGQNVLQHSIEVA
                     HLAALMASELGLNVDKVKRGAILHDIGKAIDHEVEGSHAIIGGEIAKRYGEKSHVVNM
                     IQYHHGETEALTPEAVLVAAADAVSASRPGARRESLDMYIKRLENLENIATGFKHIEK
                     AYAIQAGREIRVIVEPEKVDDLLAEKMAIDIAKKIEEELEYPGVIKVTVIRERRSVSY
                     AS"
     gene            complement(25150..25587)
                     /locus_tag="PKL93_03550"
     CDS             complement(25150..25587)
                     /locus_tag="PKL93_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNQ70369.1"
                     /translation="MLSDPMKDAVRYIKYRMRSRKEIEDQLLKKGYSQEEVDEVIEKL
                     EEQKLVDDRVFASLYVSDGLNVHHKGPFRIRQELFKLGILEEIISDSIEKELENCDLK
                     EIAKIAIGSTERTDADKMRRKLYRKGFSSSLIDEVIDEIDEKS"
     gene            complement(25587..26636)
                     /gene="recA"
                     /locus_tag="PKL93_03555"
     CDS             complement(25587..26636)
                     /gene="recA"
                     /locus_tag="PKL93_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNQ70370.1"
                     /translation="MLSKDKKNALERAIKDIEKQFGKGSVMLMGDKEEHKNIEVIPTG
                     SLAVDIALTVGGYPRGRVVEIYGAESSGKTTIALHAIAEVQKRGGIAAFIDAEHALDI
                     HYAQNLGVDVDNLLISQPDYGEQALDIVDGLIRSNAVDLIVVDSVAALVPRTEIEGAM
                     GELQVGLQARLMSQALRKISGNVSKSKCIVIFINQTRMKIGVVYGNPETTTGGVALKF
                     YSSVRLEIKRGSAIREGNTVLGNETTIKVVKNKVAPPFREAKVDIIYGQGIEHANELF
                     NLALSENMIERKGAWYTYISEDNNEVSLGQGKSNGVEFMKENPELILEMENRLRAKYN
                     LPLLEAKKEEKEEEG"
     gene            complement(26626..27192)
                     /gene="thpR"
                     /locus_tag="PKL93_03560"
     CDS             complement(26626..27192)
                     /gene="thpR"
                     /locus_tag="PKL93_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNQ70371.1"
                     /translation="MRSFIAVDTGERVPAMIDGISEKLRRMGFKASWVPGANAHVTLA
                     FLDNIELDKLALLASMLSRRLRGFPSFTLETGRIGYFKHKDLPKVIWVAIERNQNLVN
                     LQRETRVVLEALSLPVDENFRPHLTVGRMKFSPPMWRKFLSTLENEHIIFPVGEVTIF
                     ESILSREGATYRKVFTCSFEGGLIEHAV"
     gene            complement(27194..27733)
                     /gene="pgsA"
                     /locus_tag="PKL93_03565"
     CDS             complement(27194..27733)
                     /gene="pgsA"
                     /locus_tag="PKL93_03565"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNQ70372.1"
                     /translation="MNLPNILTLSRMLLTIPALLMLMSGSVVGHYFSFIVFVVAALTD
                     FFDGRIARKRGLVTDLGKFLDQISDKILVTSVFLGFMGLSRISLWFLFILIFRDTLVS
                     GIRMVASSKGKVIAADIFGKAKTVAQIALLILLYSNLLWGFPAPSVMVWFEILVAIIT
                     ALSGGNYLLKNLDIFKGGK"
     gene            complement(27720..29024)
                     /gene="rimO"
                     /locus_tag="PKL93_03570"
     CDS             complement(27720..29024)
                     /gene="rimO"
                     /locus_tag="PKL93_03570"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNQ70373.1"
                     /translation="MKLGVLSLGCSKNIADMDNFMGIMANRGHEIVEDASVADLIIID
                     TCGFIDDAKKESVEEIFKFISLKQENPGLKVVAVGCLVQRYFDELKSEISEIDGLIGV
                     TSPFTLADLIENGEFFYLKEPDGVYDFSSRVVSSKYSAYVKIGDGCNRNCAFCSIPDF
                     KGKSVSRNPDSIVKETLSLIRKGVKEIDLVSQDCTQYGKDLNEKTDLKSLLERLNNID
                     GDFWIRVLYLHPDHVDNELVDSILSLSKVVDYFDIPVQSGSTRILKKMGRIKDSDRLR
                     ELLFGIRQKAPHAILRTTLLIGFPGESEATFNETLDFVRSVKFDRLGGFVYSNEEGTQ
                     AFGQKRTIGRKAAKEMLNSLLEEQDTISAGRLSQFKGQIMTVLVEESGSSYSLGRAYN
                     SAPDVDGVVVLKGHNEDGVFVKARITDSYEHDMEGVILDELA"
     gene            complement(29021..29557)
                     /locus_tag="PKL93_03575"
     CDS             complement(29021..29557)
                     /locus_tag="PKL93_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNQ70374.1"
                     /translation="MGNYRKTEMVNLVIFVFGSYIDYRLQEIQPVLEKRFGPADYISK
                     ALDFDRYTSYYNDEMGYGLRGKLISFKRLIHPQQISLIKRLTNAIEDHFTLEGNRKVN
                     LDPGYIHHAQFVLASTKHWANRVYVGDGIYAEITLMYIDGSLKPLPYTYPNYKDREYI
                     EELMKIRELYLLKRKERL"
     gene            complement(29557..29892)
                     /locus_tag="PKL93_03580"
     CDS             complement(29557..29892)
                     /locus_tag="PKL93_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70375.1"
                     /translation="MEKRVVLKSQFVLVLMLIISLFLFVETLYIIRYVKESRSAEFEV
                     IFYDNTTPIRILLDDEGYVLQPGESLMLSIGRNDEVIVEQEVAGRYTVRGGGKIVNVS
                     IPRVKVSVK"
     gene            30039..30692
                     /locus_tag="PKL93_03585"
     CDS             30039..30692
                     /locus_tag="PKL93_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNQ70376.1"
                     /translation="MDSRKIPKPTIKRLAIFHRCLENLVISGVSSISSKDFADRLGIK
                     ASQVRKDLSYFGEFGKRGVGYNTELLLDSISEILGIRKRWNTCIVGMGNLGMALANYP
                     GLSKSGFVAKALFDNNPNKIGMPMPNNLMIESTAKLKEIVKSRNIEIGVITVPASAAQ
                     STADLLTEAGIKVIVNFAPIRLSLPQEIINEEIDISASFRSLAFSMTYGSSKKGRKH"
     gene            30692..31297
                     /gene="udk"
                     /locus_tag="PKL93_03590"
     CDS             30692..31297
                     /gene="udk"
                     /locus_tag="PKL93_03590"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNQ70377.1"
                     /translation="MVLVSITGGSGAGKTSVTNMIFNHFMERAAVLSMDDYYKNLDPG
                     TDPRQFNFDSPKAFDFELFEEHLRFVKENKSIMVPVYSMVTYRRESGICRKFIPKPLI
                     IVEGLLVMFKKEMRDLFDFSIYIDAPADERLIRRIERDTKERGRSIESIIEQYRKFVA
                     PSFESFIEPQKYFCDIVLPDGAANTTGLNVIINAIENMLNK"
     gene            31316..33637
                     /locus_tag="PKL93_03595"
     CDS             31316..33637
                     /locus_tag="PKL93_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70378.1"
                     /translation="MPKRLLSVIVFLAIAILSFSFSQKVIIEDFKAYVEDYNKEAPSL
                     PKVLKLIEDLDYLSVYRLYKLQMVGSIEKKESSTTIAGLLTRHIEAFDESEFRDIDDR
                     IAYSAFLAWVLTDISGKPFEIHTLNEMPAYLEVFNTYSSRVREMAGEVYKEWIAYSLG
                     LIETKPGLFPDELLVTDSFSSYSIEADAPYDSEKEILSLSNRSVIDALNSAISTISNR
                     EYSVSSLVDEEVNRLAVQTAMDLSQVGNAEIMSSGRELFRLWLLRSIGLIDKAPFYPE
                     SVEIRVKDIPGFEIDSIPEDSYFQDLMDILEENQELRSQIVEKIQMGAQLLSLKQFTP
                     TSLIESDIESEVRKIVPIQANIMGGIRNELSKSLVSSIDKRIDLWWLRILAYALIAIL
                     GFTVVPAMRKYVIGIIIILETLYIFFIGEITTGIFDLSLHSVVALPAFAFVFILAVAA
                     AFSRRKRSRILILKLLLLFLIALLPFMNLLNEQPELLMDNFEGFYDSVYYSTLKNDVF
                     LAPESMISLQTRDLNSLVSSELNSFKRVLRVIIPNKMNSFSTAAEMSFSVDSNGRLRV
                     AAPAFSEYMSIENQNTYVSELEGLTKDIEGFIRDSERNRKGYEAALSTLIDTSERIVS
                     FAGSRMREDFSNSLETELQSKPELEVALDDYREKMAPLLNDAPSSVPVKVYRVPEFAS
                     LVVALLLLSITLFVVRKPVISFINLAVITAYFLIVLPGIDTLNLFVQGGSPMLRISIE
                     PSVNALFLTVFAGIIALSVLWILLSHKKGSVVE"
     gene            33634..34656
                     /locus_tag="PKL93_03600"
     CDS             33634..34656
                     /locus_tag="PKL93_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ70379.1"
                     /translation="MKLKLLLVFSLILMFALPVLAMKVIMVTDVGGLGDKSFNDGTWE
                     GIVKASDFLGLEREVVQSKEQADYIPNLSNAAKEADIVFGVGFMMADALYKVAPQFPD
                     TYFIGIDIDPIENMPNNVATYLFKEQEGAFLVGYVVAAMTKTNMVGFVGGLPIPPVER
                     FRYGYEAGIRVYEELHGKTISILQGYTMDFNDPKKGKDLAIAQFAEGADIVFHAAGAC
                     GNGVIEAAAEKGEGFFAVGVDVDQDYMAPGRVLTSSVKRVDMASYQAVMSIALGTFEP
                     GAKTLGIKDEGVGISPMTYTKDIVGPTILSQVEFLKGLIKAGTLVVPDTQEKLDAFVV
                     PEITLP"
     gene            34746..36338
                     /locus_tag="PKL93_03605"
     CDS             34746..36338
                     /locus_tag="PKL93_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70380.1"
                     /translation="MDLNYVENLDLSGIAVAMKGIVKQFPSVLANDHVNFEVRKGEIH
                     ALVGENGAGKTTLMNQLYGLYTPDEGEVFINGRKTIINGPKDAIDMGIGMVHQHFMLV
                     DNQTVAENVVLGSEPRRGPLKLFMDSGRARKIVIEFSKKYGLAVDVDAYIEDIPVGMQ
                     QRVEILKILYRGAEILIFDEPTAVLTPQETEELFKILRVLKENGKTIVFITHKLNEVM
                     AITDRVTVMRLGKVTGQVNTKDTNPRQLANMMVGREVLLRVEKEEKKAGETVLEIKDL
                     HVKDNRNLDTVNGVSFVVRRGEIVGIAGVAGNGQTELVEAITGLRKVEKGRIFLNGED
                     LTNHTALEIREAGMTHIPEDRHKRGMIMQYPVYYNLILGKQDEEPFSQEGFINLKAVK
                     AFASDVVETFDIRPKNINMIAGNLSGGNQQKVVVGREISIVPIEPKVVVVSQPTRGLD
                     IGAIEFIHKTLISMRDKGIALLLISMELDEIFSLSDRILVFYEGQIMGEVAPHEVNRE
                     EIGLMMAGHKKTEVVRERSDES"
     gene            36335..37390
                     /locus_tag="PKL93_03610"
     CDS             36335..37390
                     /locus_tag="PKL93_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70381.1"
                     /translation="MNTGKIYALLVPIVSVLIALLIASIIIIMIGKNPVTAYAIMLDG
                     AFGSQEAIADTIMKMTPLILTGLAVGFGFRAGVFNIGAEGQMTMGALIGAVVAMNIGG
                     IPSVIAIPLSILAGMLAGAFWASIAGFLKAFTGAHEVISTIMLNWIASNLASYMVTGP
                     LAVGSGTPKSPEIAEASKLPVILKVQATELSIGIFIAVAVAIIMYILIEKTTTGYKLK
                     AVGFNPHAAEYGGISIRKSIILTMAISGALAGMAGIVDLIGVPPHRFVGELTGGRGFD
                     GITIALIGRNNPIGIIFAALLIAALRTGSNAMQIRAQIPNDIVSIIQGIVIFLVAAER
                     IVSTLISWKRKKGVTAI"
     gene            37387..38346
                     /locus_tag="PKL93_03615"
     CDS             37387..38346
                     /locus_tag="PKL93_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70382.1"
                     /translation="MSWIEAIFAILVNPLFYKLTLLSATPLIFAALGGTYSEITGVTN
                     IALEGIMLMGAFTSIVFTLITGNAWLGVLMAVIIGTGFTWFHAWASIRWSANQIVSAT
                     ALVIIAQGTTSFLMIPIFGNEGQTDFIGRVPYLDAGWLSEIPFIGDIFGSMSPFTYLA
                     IIAVIASWFLMYKTPLGLRMRAVGENPEAADTLGINVFKIRYFGVLMSGVFASLAGAF
                     LSVGELGRFVENMIHGRGFIALAAMILGNWNPVGAMWAAILFGAAEAMNLQLQSSSIV
                     SVSSSVKPLFNMLPFVVTLIVVGGFIGKTRSPAASGTPYEKDS"
     gene            complement(38380..39828)
                     /gene="gltX"
                     /locus_tag="PKL93_03620"
     CDS             complement(38380..39828)
                     /gene="gltX"
                     /locus_tag="PKL93_03620"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNQ70383.1"
                     /translation="MIRVRFAPSPTGYLHVGGARTALFNYLFARHYGGKLVLRVEDTD
                     ISRSTKEYEDQLMESLLWLGITWDEGPDVGGAFGPYRQSERGPIYREMIGDLLQREQA
                     YEVHAYPEEIEELHDKLLSEGKAPHYDQEMLEAFNTDERISEFESKGLRPVVFFKMPR
                     KEYRLPDLIKGEVVFKEGAIGDFVIMRSNGQPIYNFAVVVDDITMEISHVIRGDDHLS
                     NTLRQLALYEAFGAPIPSFAHVSMILGPDGKKLSKRHGDTSVEQFRERGFLPEAFFNF
                     LTLLGWSHPDGKEILNHEEIVESFSIERVNTSAAIFDEAKAKWMNGVYIRESDLERIT
                     DLAVPFIIQSKLMTAEDAVANRKWLKRAVDSVRKGVETLSEIPDKMRLYFEDPQVTFF
                     TPSNDVEKGVYKALEAFKDRVEYLDSWSNDEIIGTIRGILKDLKPDRKAFYMTLRKIL
                     TSVEEGPELVDIIFLLGRNKTLNRLQRAINSI"
     gene            complement(39821..40357)
                     /locus_tag="PKL93_03625"
     CDS             complement(39821..40357)
                     /locus_tag="PKL93_03625"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNQ70384.1"
                     /translation="MERSVGEERIFSGRILGLERHTVILQDGSLALREVVRHPGAVAI
                     VAFVSEQLLLVRQFRFPVQKYMLEIPAGKLDAGERPEDCARRELLEETGYVPRFLHKL
                     ITIETSPGFSDEIIHIYVGEVEKSQEPCPDEGEFVEPVLVDSKEVVKMIIGGQITDSK
                     TVSGILLAFAGRESDKYD"
     gene            complement(40359..40751)
                     /locus_tag="PKL93_03630"
     CDS             complement(40359..40751)
                     /locus_tag="PKL93_03630"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNQ70385.1"
                     /translation="MIDRLEEKALIQKAIEAKERSYSPYSKFPVGAALLTEEGRVFVG
                     TNVENSSYGLSMCAERSAIVSAVSNGYRKFRSIAIVSDRKEPTSPCGACRQFMVEFGN
                     FEVILVGEENIKRTTTFELLPMHFEMEK"
     gene            complement(40705..42072)
                     /locus_tag="PKL93_03635"
     CDS             complement(40705..42072)
                     /locus_tag="PKL93_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNQ70386.1"
                     /translation="MEDPTSSGDPVSILLNLLLLGFLLYLSGVFSGTETALMSMGKLK
                     LRDLVDSKEKRFSKSVKFFVQNPNSVLTAILVMNNVVNILSSSLATLLALQVLPDNSA
                     GVAAALVTGIMTFLILIFGEITPKIYARENSERLFRRTITFISAITIVLKPILWLLLR
                     ISSFFIIIIGGKKAEFSPFITEDEIKSAVDAGHKEGVLQSEERMIMKRTLELKDISVK
                     EIMTPRVEIVALEEEQPLIDLMELVQSEGYSRYPIYRENIDRIVGVCYAKDLLNFILD
                     RKDNDVLQTIRVEEIMRYPYFVPETKKVDDLLREFKEKKNHLAVVIDEYGGTAGIITM
                     EDVIEELTGEILDEYDEESEEIMIERLGEREYIVDGMTPINDIERELEQPFPETEFET
                     IGGYLLEVLERFPEVGEKIIVDGFTFEVLAAGKNKVEKIRLIVDRRSVDDRQIGGEGT
                     NTESY"
     gene            complement(42093..42560)
                     /locus_tag="PKL93_03640"
     CDS             complement(42093..42560)
                     /locus_tag="PKL93_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNQ70387.1"
                     /translation="MNIRIVGTGKPKSNFIVEGVEQYRKWLGAFGRVDLEYFPLGGTT
                     AREREQVKEREGRRYLKAADSQDKILLLDAQGEQYSSQEFARMLEKWQNIGVSNLSFF
                     VGGPLGFSNEVLSKGWQKLSLSRMTFTHEMAVLILLEQLFRAFAILGNRPYHY"
     gene            complement(42557..43771)
                     /gene="hutI"
                     /locus_tag="PKL93_03645"
     CDS             complement(42557..43771)
                     /gene="hutI"
                     /locus_tag="PKL93_03645"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNQ70388.1"
                     /translation="MVKADLAILNIGQIASPKKAGPIRGKEMSSLETRHNMHIAIEGG
                     KISYVGRENVEAREYIDGTGLVAIPGFVDSHTHIPFVGSRSKEFLMRLSGKSYMEIMK
                     TGGGIRSTVKEVRKASASELVAESLRHLDRMLSLGVTTIEGKSGYGLDKENELKQLKV
                     LKTLDSIHPVDLVPTFLGAHAVAEEYESSDSFLYSMAEMLDDVSEYTDTVDIFCENGV
                     FSENQSRWYLKRAKEKGFKLKLHADELSASGGGRLAVELGALSADHLISADDGTLELL
                     SNSNTVATILPGTSFFLNEAFAKGRELVDRGAIVALASDFNPGSCNIYDPFFIIFLAV
                     SRCGLSVEEAIIAYTVNGAKVLCLEDRKGRIEAGFDADLVLIKAGDYSEIVYNFSRNI
                     VSGVIKSGKRVR"
     gene            complement(43771..44685)
                     /gene="ftcD"
                     /locus_tag="PKL93_03650"
     CDS             complement(43771..44685)
                     /gene="ftcD"
                     /locus_tag="PKL93_03650"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNQ70389.1"
                     /translation="MRLIESVPNFSEGRRLDVVERIVEEAKKIEGVWILDYSSDPDHN
                     RSVITITGEPEAVEAALFNMTLKAKELIDLNNHSGEHPRMGATDVIPLVPVMNVTKEE
                     CIALSKKLGKRIGEELRIPVYLYEDSASTPERVSLSNIRKGEFENFASKIASDQWKPD
                     FGPSEIHPSAGVTAVGCREYLIAFNVNLGTNKIEIANKIAKSVRHISGGFRYVKALGF
                     KLEDRDIVQISMNMTNYRKTPLFRVFEVIKSEAERHGVPIVGSEIVGLTPLQALVDVA
                     DHYLRLEKFTSSSILEKKVLDFIADKER"
     gene            complement(44706..45794)
                     /locus_tag="PKL93_03655"
     CDS             complement(44706..45794)
                     /locus_tag="PKL93_03655"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNQ70390.1"
                     /translation="MKRRVEFLLPIIMVSLMIFGLVVLYSATFGESEEYLFGRQLVWV
                     LLSCAVFVFTAYVVKKELWKSLSFPLIILSTVSLAAVLYLDSGEASRRWIDLGIASFQ
                     PSELAKFSLIIFLGNVYSRDTKHRFGLFSLGIFFTLVFVGLIYLEPDLGTTIIVLIIW
                     YFMTLISKRYDRLMIAITVLIAFLAPIVFMFGLKPYQRDRLLSFLHPEEYASGAAYNT
                     IQAIRAIGSGGLKGTGYLQGTMNRLGYVPADHTDFIFSVLGEELGFIGAVSLLVLYLL
                     LLWRVWSITRKTGSEFGRIVLSGIFAVFAFHVVENVGMNLGLLPVTGIPLPFVSYGGS
                     SSMLFALQLGIVHSYSVKEIEYDKEQRE"
     gene            46019..46819
                     /gene="panB"
                     /locus_tag="PKL93_03660"
     CDS             46019..46819
                     /gene="panB"
                     /locus_tag="PKL93_03660"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNQ70391.1"
                     /translation="MNVREIVQMKGQSKLSVISAYSYFEAKFAEEAGLDMIIVGDSLG
                     TLVMGKRDTLSVTMDEMIWSLKSVRRGAENSFIVADMPFGSYQCSREKAVENVVSFLR
                     HGASAVKIEGGFETANLIKHLVDSGFPVMGHIGITPHHASLVGEYPVQGRDEKSIKRL
                     IESVNELEEAGVFAILLEMVVEDVAKRLTEDSSVPTIGLGSGRYCDGQFLRWHDLLGI
                     NDESSPRYIKRFADLKSNIISALRRFDEEVKSGVFPEEKNAFEGAEEI"
     gene            46791..47594
                     /locus_tag="PKL93_03665"
     CDS             46791..47594
                     /locus_tag="PKL93_03665"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNQ70392.1"
                     /translation="MLLKGQRRFDVFLQIRSDFEEEIPTMMVGENIVSFSKIDSTNMY
                     AKNNLDKLPSGTVVWALSQTSGYGRHNSFWYSNTGGLWFSIVFKPSLIREPNEYTKVV
                     SVAVAETMIKMGINSVGIKWPNDVFIGKKKVAGILTEAIFSGISVNAIVVGVGINVNN
                     ELPEEIRQIAISLGMVINKHISLEMLLDKVLKRVEFLRKNYLIRGKNRYLTRRWKKYL
                     AFAEKDEITVSLPSGEKITGIINSITPSSLCLEREDGEILKINSGEILL"
     gene            complement(47569..47808)
                     /locus_tag="PKL93_03670"
     CDS             complement(47569..47808)
                     /locus_tag="PKL93_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNQ70393.1"
                     /translation="MRIDKFLKMNGIIKRRVVAKQAIEKGYVFRNKIRAKPSSEVEPG
                     DLVSVRFFNRVLVVRVKEGFESEIVEETRVESPRS"
     gene            complement(47919..49253)
                     /locus_tag="PKL93_03675"
     CDS             complement(47919..49253)
                     /locus_tag="PKL93_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70394.1"
                     /translation="MRRFMVLALLVIGALSVASVGTMSGRLPAQSHTVLIVEDLENNY
                     NVSKKAVLMDMVLNQLAIEQLIAQYVEMLAYNNGLDPKDIYKAFDGDLGLAVWVDSEG
                     KDRLVIVLGPTENPKALKAAIEKILPQLLPSETELNIAVDQEYLFIGDIEQYAVTEKG
                     FNLSLVTEGLTPGFAYFYSSVGDVVQKGSLWNEEGILIAEALSIPLSEETRERFSSAL
                     EGASVVELEAGYHLPLVSGFAKINDLSFLSDLNIEQLGLLPLELGGLKIGTQDITEFS
                     EEMTGEIMIDANISVDDIFASLMGTEGTTSGATDSVGYSYVLRAGYSGTLDDLKSRLE
                     KADPDLKILVIENGLSIEDQYIWVSDGWLYVSSSNQVKITKELQEGAIVSDLEVYNEL
                     LKFSGSEGFARLFLDSGYILTGLMGVEIESGVLVNSQYVREIGGIRSTIVIK"
     gene            49501..50637
                     /locus_tag="PKL93_03680"
     CDS             49501..50637
                     /locus_tag="PKL93_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70395.1"
                     /translation="MRFLRLVKNDLLLIVKSRAFLFVLILLPVILAAISSITSETGGY
                     SDLRIGIINEDKTFIGLFFVQYATSMLKGENILQLSNREEIDSVIDKLDGVFIIPRGF
                     ANKLLFQEPSELVFIPNPGSMQTAVAVYQVLSNVLREFKALPVVADPEFMKTVEIDPN
                     YVAPSLTVEGISESKLSFSSLLFPIVLVTTIMFITSIGAATGIYEDRRNGVIDLLRLS
                     DVGSVKYIIAKTVSFLIFSTAQVLIFITAGRLFGLEIASNLILYVVVLEVYILLFTSI
                     GLLIGTILNSSRSAQLTSITLVTAVLILSGILIPHSMFPDWLESFSRSLPTTSLLVVL
                     QGISMLDFTFPMVVLPTAINLLIAIAIIILTGFVASREDISLAD"
     gene            50753..51211
                     /locus_tag="PKL93_03685"
     CDS             50753..51211
                     /locus_tag="PKL93_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNQ70396.1"
                     /translation="MEDLKKELFELANEVASNLGYSIYDLGLGRRGKKRLVKITIDKL
                     DGYVSISDCESFSADFGRALDSADLVPFSYDLVVESPGLERALRDLKDYVRFTGEKAK
                     VILKEPIEGVSVLIGKIISCENDVVTVESSENGKEIAFHFSNVKRANLKL"
     gene            51233..52261
                     /gene="nusA"
                     /locus_tag="PKL93_03690"
     CDS             51233..52261
                     /gene="nusA"
                     /locus_tag="PKL93_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNQ70397.1"
                     /translation="MNLNLLEALDQLQDEKNIEKEEVIDILEKALQSAYKKNFASESD
                     VDVRIDRLTGDIEVFEKLLVVEAVENPSLEVKLEEALKLDSSAEIGSTVERKLNIKKF
                     KRIAAQTARQVLIQKIREKEKENLFDKYVDMKGTVTTSEVLRVTDEWIDLRIGKLETR
                     IPTKELIPGEQPRLNSLMKVYVVDVTKSVRGPRLLVTRRTPDFVIELLKLQVPEIASG
                     DVTVKAIAREEGIRTKVAVFSQNTKVDPVGACIGESGTRIAEVLREIKPEKVDILRWS
                     DNPAEFVGNSIAPASALEVKVANIENHEATVFVSPTQLSLAIGKGGQNARLAAKLTGW
                     KIDIKPIM"
     gene            52288..52755
                     /locus_tag="PKL93_03695"
     CDS             52288..52755
                     /locus_tag="PKL93_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNQ70398.1"
                     /translation="MRNDQLKAELKKRNQRMTAQRELILRLFLEAGTEHLSADEIYHK
                     VQGKNFRISKATVYRTIELLADIRLLRKIVFEDGVVRYELVEKGEHHHHHVICSNCGA
                     VVEFHLDNLEELEELVKSRTGYTIEDHQLKFYGLCPKCQNAMKDKKSTSNEIK"
     gene            complement(52686..54047)
                     /locus_tag="PKL93_03700"
     CDS             complement(52686..54047)
                     /locus_tag="PKL93_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNQ70399.1"
                     /translation="MHDALRRRSTPFPFLFYLLATMLGELIIFSSIWMDSGWLSLALL
                     ALSVMLISSKQVSPFAWVLFFVMGCLVQTFTLKMLDPGKVEYVGYVSKGGTGRIEASN
                     GRLLRDGEWIYLPPAVNIDLSDRKAVAFPGQIVWSAGRLKRSDTYPLLTIESEVEGCI
                     DRFEIISLPGKHIAGLLSKYELDNTIAAAVFTGDRKHIEYELRRTISDLGIAHLFAVS
                     GLHVGLMYLLVHSALSFLMVGRNSRIIASISLLFLYTLSTGPAISALRTFLMLLCYSI
                     FRIIDYRQHPLNILGLSGMIIVMVQPSIVASISFQLSFFATAALLIFLPEIEKKSLIY
                     QAFLVGAIAQAAIVPLSLTAFGTLSLVGIPLTILMVPMFVMPSYIGMIAILVSDFLGI
                     EVVSSFISGSLRTMSNIMGKVTASIGEAIPAMRFEAFPAYFISLLVLFLSFIAFWHLG
                     HKP"
     gene            54146..55213
                     /locus_tag="PKL93_03705"
     CDS             54146..55213
                     /locus_tag="PKL93_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNQ70400.1"
                     /translation="MRQFSFGGLTLVGYYGYDNLGDDLLLLSSLSLIEEIEFEGPIYL
                     PASPSINHIAHRFPSKLDVRIIKRFSIRELQISIKNSILTVFGGGNLLQDQTSWRSFL
                     YYYEIAKYTLKKDKPLLFLSQGFGPLSKTANRKALNKLLSDPLTYGVMRDAVSYKHFA
                     SISNKAILGTDYGPYYLRKKRIIPEKRETIDGLAVIVLKNGTSVDDVLHSLRLNNLSQ
                     VCAVGFHNHHDEQKRSELESKARSNGFKVRKPPENLEGMIEVFNNAELIITERLHGVI
                     LAISLGVPFVWKKNTKLDRFVRSLDKNCNLYFEESCESLSLSINSSLKKPVNLKEDYW
                     SRLENTVNESKELLKKLLARR"
     gene            55218..56315
                     /locus_tag="PKL93_03710"
     CDS             55218..56315
                     /locus_tag="PKL93_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HNQ70401.1"
                     /translation="MWKYFLPTRVFAGRQVIGQNGELLKEFGTKAFIVTGKSSAKKSG
                     ALDEVLRMLEALDIPSSIFDEVEENPSFFTVEKGGRLLSENECDFVIAIGGGSPLDAG
                     KAISAVGMNGCSCEDLYRGKNLPSHPIVAIPTTSGTGSEVTQYSVLTNADGQKKGFGM
                     PSIFPAISFLDPRYTVTMPLDVTIATALDALSHSLEGEVVNKGQNPLLKTLSREANRL
                     IKDSLDRILLDEDDIDLRERMQFAATLAGIVIAHTGTTAVHAAGYPLSSFKNVKHGMA
                     NALILVEIFKKIAEADKDRIGGAIDPFESLDELQIFIQRFISGRISLRISDEEINEWS
                     DYTSKASHLKKTPGDFDKEFFIDLYRRINNI"
     gene            56312..57490
                     /locus_tag="PKL93_03715"
     CDS             56312..57490
                     /locus_tag="PKL93_03715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNQ70402.1"
                     /translation="MSARVVLKRSIKRRIAFGHPWIYDNEIEKADSVEGGCIVDVLTN
                     SGQFLGRGYYNPDSSIRVRLLTRRNCSIDTDFFAQKLKRSLGMKSSLLRYGNALRVTF
                     GEADGLPGLIVDKFSDWLVVQFNTLGIGVLKNEIIEALIATLKPKGIFEKSEGISLTK
                     EGLESKEGWLFGNGPELLPFSLNGIHFFADTKGQKTGFFLDQRDNAENLSKYANGRNV
                     LDVFSYTGNFSFHCLSQGANRATLVDSSERALSVARETAEINGFISRCEFVRANAFDY
                     LREESLAGHSLVIVDPPAMAKSPSSKKSALRGYKELNLRVIKKAVNGSLLASSSCTQI
                     ISEDDWMRTINDAFHDSKKVGIVSFRGGQPLDHPEVSSIFETRYLKFCLFRVIELCDF
                     "
     gene            57568..58380
                     /gene="minD"
                     /locus_tag="PKL93_03720"
     CDS             57568..58380
                     /gene="minD"
                     /locus_tag="PKL93_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNQ70403.1"
                     /translation="MAKVYVVTSGKGGVGKTTITANIGCALANKGAKVCLIDADIGLK
                     NLDITLGLENRVVHTILDVANGKVTASEALVRHKQIKGLYLLAASQIATKEMLSPEDM
                     KKMVAELYPKFDYIIVDSPAGIERGFRNAIAAAEKALIVTTPELPAITDADRVIGLLE
                     NSGMQETNIRLLINRFKIQMVKRGDMLTREDIQGNLAIDLIGIIPDSDEVIIATNKGV
                     PVILNGNGEGIGKVFENIALRMQGEPIPVEHDILEHGSKGFLEFLKRIFRRN"
     gene            58397..58672
                     /locus_tag="PKL93_03725"
     CDS             58397..58672
                     /locus_tag="PKL93_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNQ70404.1"
                     /translation="MFFGLFRKKKKTEGSRKEAKDRLDSITGGRRYSVPVREVIPQDI
                     LQNSGKDMVHQIKTYVADKYKVKEENVKVQLEEHNGYVVIITNVVFH"
     gene            58679..59068
                     /locus_tag="PKL93_03730"
     CDS             58679..59068
                     /locus_tag="PKL93_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNQ70405.1"
                     /translation="MRYSVKTERKSQFIDITKLVIQSVKESKASYGLVNVFVPHTTAA
                     VTINENSDPDVVRDILFWMEESIPENREFKHMEGNSDAHIKASLVGSSVTVPFRDNSL
                     MLGIWQCVYFCEFDGPRNREVIVTVVG"
     gene            59132..61882
                     /locus_tag="PKL93_03735"
     CDS             59132..61882
                     /locus_tag="PKL93_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ70406.1"
                     /translation="MCKGRYFFYTIFVLGSSLFAIKGFTWDMGLPKFYFASVAISVFI
                     VFVSLRAVRGSYKLSISFPQVLALLFGIYACLTTFQLLDTLPNVFLTSLGFAMNLLLF
                     LLFSLLISGEKPDRLMFILQVFVLAGLVIAVDALFSFYTGHGLLWGTENNPFTRGNLS
                     SVIGNVNFTTDLMAMLLFPTAFLTISSRVLWKREKLRHIFYLGVFSIFLIVILIGQTR
                     AVYYSVIGSFIFLAVFLIISLLKFRLRPFAAAFNKMFLILLLFAAIIIMIVYSGDNFL
                     TGGRFSFSERLTYTTEDNISVDVRVLQWKAAIKQWKSSTIFGTGFGSYKYLSTENMGR
                     VLQEEPDYMYVAGLNSIRTHNEYLQQLGETGIVGMAIVAWFILSMFLYTIRVVRRTSS
                     IDKIVQYIFLTAGLLVIFAHSTLSFPGHLMPNALFAVFLFGFLMSPDFNEERRITLDV
                     GKLAPLILVIFALTTTVLMSRTFFSEGFFTKGYIDYRRIEAINPQVPQLISSIESIKK
                     EIKALENLEDKYANLQEGQYISERLKTLQEMYPSAPTELLESIASEDREEAYESALSG
                     LNSKLRSASSALLNARRDSTESFYSALRNLSTAREISGSQYLAEAYLGYVYLTPQRKE
                     DFRLKLNMSGNNIGNAFGEIFSRKDIFSSWLTYDALPCGMLPGLEIDHAYLKELQSLL
                     KPDLAATDVSNLLKEVSTELLIDYQVTLDAIDALLRSIKTSPDLQVVRNAASLLFRVI
                     VSSERVSLELEKLEPYVTDTVGLNNLVHKIQEIPVSEKDNLNLLYDVAIAYNPGGWLK
                     GNDSIYGEYARNLLLAYGLNALDKILELAEKEVFAWDVMRTTDQVVPLGAIGELTPLK
                     EYVPREWFHNLYDGVYEWCLDKSSEISRNIEEERFAGENLKKAETVLNEMERFKGLYS
                     TW"
     gene            61983..62720
                     /locus_tag="PKL93_03740"
     CDS             61983..62720
                     /locus_tag="PKL93_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70407.1"
                     /translation="MLEVKGIKLVRDEREILNTLTATFESGRVYAILGNNGVGKSTLS
                     YVLMGLESHRDYEGNVVFDGEEIDLLSVSERARKGITLGWQEPVRFQGLTVKEYLTLG
                     GKLKLSKEELNETLSVVGLNKEYLDRFVDESLSGGERKRVELASIILVNPKVTILDEP
                     DSGIDIMSMEMIERVLERLRDKGSIVIIITHREEIARMADEAYLLCGGRILASGEPEE
                     IVNFYRKLCDNCQHINEPITEGGRVDD"
     gene            62713..63669
                     /locus_tag="PKL93_03745"
     CDS             62713..63669
                     /locus_tag="PKL93_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNQ70408.1"
                     /translation="MINSNFEREFTAIAKEYERSGGNVSDFLRKDIVSIIVSGNKVIG
                     RNTVDGVHVKAKELNNGVEIWLEIDDGTVIDNPIHLCTGYLKPEGTQEILIHNHLGDH
                     TKAKFISHCVFPSGVNFTHSMVADTDVGKYSEMLYEDTHMHSKDGGVTVKATYNTVVR
                     EGGSFQNFFYLTKTRVGKLFVKMNVDLEKNASAHIESKVYEREDDYLEIDEQLYLNGE
                     GSSGIAKTTVFATDRSKAKIINKAYGNAAYSKGHIECNEIIKGDSVEVGTVPELYVRN
                     EKAELTHEASIGRVNVKQMETLMSKGLTEEEATDMIVRGMLR"
     gene            63687..64715
                     /locus_tag="PKL93_03750"
     CDS             63687..64715
                     /locus_tag="PKL93_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNQ70409.1"
                     /translation="MNYSELYIVKELVDLVNIPSPSGFTDSIIVYLDSELKRLGFSPR
                     RTRKGALVVEIGGRGNPIVLAAHVDTLGAMVKSLKPNGRLEITNVGGFTMNSIENENC
                     IIHSKTGKTFSGTIQSISPSVHVFENARSLERKIANMEIRVDEELKNAEDLQKLGIEA
                     GDFVSFDPRANVTKNGFVKSRHLDDKASVAVLLDFARRVSEGKVTCSRKTYLFFSNYE
                     EVGHGASAAMPDDSEEIISVDMGAVGDTLGTDEYVVSICAKDSGGPYDSTVVRSLIET
                     AKRVGADYSVDIYPFYGSDVEASLRAGYDVRFGLIGPGVEASHGYERTHYKALENTLK
                     LIEGYIGN"
     gene            complement(64741..65166)
                     /locus_tag="PKL93_03755"
     CDS             complement(64741..65166)
                     /locus_tag="PKL93_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70410.1"
                     /translation="MQVSLDQFLGAIMSRVDNLEATIDDLRLRTNIAMRLVKSVVPEL
                     TRDDVEKAVSEELEITKNAGMMEESDEIEKISSRLTDSIFEWLQGDVAELREKMDAYR
                     KKLQEAMEAEKNRVIDVAPAGFVNQLGQEKGQKKGKLLF"
     gene            complement(65253..65807)
                     /locus_tag="PKL93_03760"
     CDS             complement(65253..65807)
                     /locus_tag="PKL93_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HNQ70411.1"
                     /translation="MRLLLVQESLIGMLAVSMFFWSASLSSAVLLIAIVLGLLVYRPE
                     LENKIVSKDSLLEMVRGVLKRPLTMVLIVTIIVLRNPGAYSILAIISSICLVTSLASV
                     SFLIFKELRRIRENVLAIFITVASIISGWLFSDFLSAPFFSGKFLPSFWIFKISEGDL
                     VGFLMVSVLSIWLFRMRHRLDKNT"
     gene            complement(65810..66571)
                     /locus_tag="PKL93_03765"
     CDS             complement(65810..66571)
                     /locus_tag="PKL93_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70412.1"
                     /translation="MIEARNLTRKFGTLVAVDRLNLNIASGEIYGFLGPNGAGKTTTI
                     KMLTGIISPSLGEIFIDGLDMQKDLMEIKRLISVVPDEPKMYENLKGLEFVKFIIDVY
                     RLPVKETLKKLNNLAEAFKIDYLGKYIGDYSHGMKQKLMVAIALMREPSVVFLDEPTV
                     GLDASSAGKLRILLRNMADNGTTVFMTTHVLEVAEKMCDRIGIIDSGRLIAEGTLSDL
                     KESFGRAESSLEELFLDITGDGETKLSIEGFEGEA"
     gene            complement(66568..67917)
                     /gene="dnaB"
                     /locus_tag="PKL93_03770"
     CDS             complement(66568..67917)
                     /gene="dnaB"
                     /locus_tag="PKL93_03770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNQ70413.1"
                     /translation="MSDLKTRVPPNSIESEEAVIGSILIDPDVVPDVMELLTGRDFYS
                     RKNQLIFSTMEKLFDEGSPVDIVSVTERLRTGGVLEEVGGEVELAKLADVVPTSANFF
                     YYGKTVKEKSLLRSLIAAASSIVENAYEGEDTDEILDNAEKAIFQITEAQISKTYQHI
                     GKIMHELFHSLERLKENALTGRITGIASGYRRLDQVTTGFRPSDLVIVASRPSMGKTA
                     FALSLASNMALKFQLPVAVFCLEMSKEQLAQRLLCNVTNFELSRLRAGDIGSEEWKRL
                     TNGASTLQTAPIIIDDEPSLDPRTLRAKARRIKMEHNIQVLFVDYLQLMHTKSRSDNR
                     QQEISEISRSMKLLARELNITVVALSQLSRAVEQREDKMPRLSDLRESGAIEQDADTV
                     MFLYREDYYKDKSEVSDSPQLTKVIIGKQRNGPVGTVELMFHPKTATFYDKAFEAEK"
     gene            complement(67935..>68762)
                     /locus_tag="PKL93_03775"
     CDS             complement(67935..>68762)
                     /locus_tag="PKL93_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNQ70414.1"
                     /translation="SEMDAETVGFQMGEEYTTIDLSLGILKDDMIDYVERLCNNFIGE
                     DRAVRKFVVESSEIQKYRLRKEIKREITESEREIRLVEIDGIDLSACSGLHVDRTGQI
                     GLLKILKHEKVKGSLTRVYFVAGKRALNDYFTKHKLITNSSLLLTCSYLDLPDRIASL
                     INEIKQGNSTIRSFSERLAGYVAEGINGSKSRVIFLEDEESILASIPRFVTLEEYLII
                     GKSDDRILLFCKGYDCREVLTRIKKEFDVKGGSGKERGQFVFDGEFASIKELIENSY"
BASE COUNT        19356 a        15770 c        14203 g        19433 t
ORIGIN      
        1 ggagagctgg caacccgcaa cgggttgctg gctcttcctt tagatgagat aaagatgaga
       61 gttgagagat tcggaagatt tgtacgtcgc tctgaagcag aaaacgatga atcgatgagg
      121 cagatcatac cgtacgcggt tttcaggaat cgcgacgagt atcttctcat gaagaggaca
      181 aaaaagcagg gcgaagctcg ccttcatgaa ctatattcga ttggagtggg tggacacata
      241 aatccagagg atggaaggct tccatgggag gcctttgaga acgggttcca gcgcgaaatc
      301 cgcgaagaag tatctgtgaa aatccacagc atggattatt tgggaatcct aaatgatcta
      361 cagactgagg tcagcagagt ccacattggt gtagtttata tagctgaagt ggatttcggc
      421 ggcttcaacg aaaaggaaaa attcactggt gcaatggctg aaattaaagt tctttcagaa
      481 tacagggaga gtatggaaac atggtctcaa atagttctag attatcttct gttaaactga
      541 gatgcccggc aaaaataaac ctttaccttg cggtaggtaa gagcagaagc gacggattcc
      601 acgagattag cactgtcttc cagacaatcg atttctacga cgatttgttc gtaaagcatg
      661 atacaaggaa gttatacttc aaatgcaata catctctccg ctgggacaag gagaacactc
      721 tttataaagt cattgagcaa atagagagtg agatagaccg gagaatagaa ataggtctgg
      781 aattgaagaa gagaatcccc tcgggcgcag gactgggtgg gggaagttca gatgccgcag
      841 cactcctgaa gtatttagcg gggcttttca acattgatga agagcgagtc tcgagaatcg
      901 cctcaaatgt tggcagcgac gtacctttct ttctcaaagg aggaacggcg atagcttcag
      961 gaaggggaga gatcttgagt tttcccggtg atgtctctgg atattcagtc gaactttcct
     1021 tccccggcat tgaggtctca actccccttg cttacgagtt catagatgaa gataaccccg
     1081 aactgcatct cgattgtgaa atggctgaga aatactacct tgcccttaaa agtcatgaca
     1141 ttgaagctgt aaggcgtatg tcttacaata cgttcgaaaa gtccatcttt gatcgctttc
     1201 ccatgataag agattgccac tcaatgctct ctaataagaa tggcggtgcc attaagacaa
     1261 tgatgactgg ttcgggctca gcgatctttt ccctttttga gggttgcaag ggaagataca
     1321 gttttgttac ttcagaggag ctgaatgaga aatggtgttc gatggtctga ttctccagag
     1381 agtcctggct gaaatatctg atgtctctgg tcagcaactg agacaggtat accaaatagg
     1441 aagaagcgag ttcttcttca aattctcgaa aaggggaatt gaaatctctc tcaatccatc
     1501 atcgccttat ataattgaga gcaacagaaa tcagaattct ccatcgatcg agactccttt
     1561 cagtctcttt ttgagaagac acatcaacgg cttctttctg acaaaaatcg ctcagttaga
     1621 catggataga ataacaagaa tggattttga aggtagagat gcctttggag aaagaaacaa
     1681 atactcggta atcgcagaat tcattgggcc agggtctaac atcatggtac ttgacgagga
     1741 ggaaagaata ctacaggtat ttagagagat gatcagctca aagagaactc tcgccagaaa
     1801 tgtgaagtat tatccgccag atagtccagg aaaatctctt agaaacctgt ctaaagaaaa
     1861 tattactcta acgctccttc actcaggaga taccctttca agagcaataa tgaagtcgtt
     1921 tacaggcttt tcgagagcaa ccgcagagaa tatcgtgggc ttcttgcaga ttgaagatgt
     1981 tgttccatcc agtcttcggg aagaacgtct tgatgaagtt gcagaattcc taatgagcct
     2041 ttctctggac accaacggaa gatcgctttt tgtgattgac gggacaaagg gagctgaaat
     2101 ctcacccata cctctcgatc ataaaggccg ttgcgaaaag gttggggcgt cggaagctat
     2161 tagacgcgca ataaaatctt caggagcaga aacagaggtt gacaggagaa agcagtcaat
     2221 aataaggaag attgacaaat cgttcaaacg tcttttcaag ttgatcgata agttagaaac
     2281 ggaactgtca gaagttgaga attacaggga tttcaaaaaa tacggagaac ttattaccgc
     2341 aaatctatat aggctaagag aaagaaggga aacggtggag cttgaagact gggagacagg
     2401 tgaaacagtc gaaatctctc tggacagtag gctaacgcca tcggaaaacg cccagctttt
     2461 tttcaaatat tacagcaaat ctctgcgcaa ggagattcaa ctaaagaaaa ggttaagagt
     2521 tttgcgtgac gaagtaaact accttgatca gctaaaggaa atggtctcac aggcagaatc
     2581 gctggaagac gttcgggagt tctcttctga actggaagaa gctggcataa gaagaaaaga
     2641 aaagatagat aagaagagaa agaagaggtc acagaagtct ggcccgagag tcttcgaaag
     2701 aaacgggttt aaatttctcg ttggaaggaa caacattgag aatgatgaaa taaccaagaa
     2761 cgcctcaagg gacgatatct ggttccatgc aagaggcatt cccggagcac atgttatact
     2821 taaaaaagct ggaaaagaga tcactccaga ggcgattctt tatggttctc ttctagcagc
     2881 aaagtattcc aggggaaggc agtctggaaa ggtagaggta gcacatactg aagtgaaaaa
     2941 cgtaaaaaaa ccaaaaggag ctaaaccggg aatggtcaca tacagaaagt tcgaatcgat
     3001 tacagttgat cttacgaaag agatggtggg aaggaattga tcagacttcc accttatatt
     3061 ttctttgtag gcggcttctt aacttacgcc tcgatttttt tctcaagcgc cacggtatca
     3121 atgacaatgt cggtaatcgg aatgacgata agtctttaca tatggtacat tcttgcgtgg
     3181 aaccgcgatc gtcatcttgc caccatgaag aaaaaaggtt acgtcaaacc gcagaattgc
     3241 gcggagcaaa agatcgcggg aaattctaga atatgggtaa ttctttactc ggcttcatac
     3301 ttaacaatga attttgccgg tttatacatc attaaggcaa ttattgaaaa cattgatatg
     3361 agtcttgata ctatgagcat ggaagagctg atcaccttgc tgggtagtgg atatgttctt
     3421 tcaagctggc tgttcttcat aaccggcata gcttcgattt ttctttatgg gaagctgata
     3481 acaatgctgt acaacgatga gatgaagatt cagtcactcg agagtaaaca gagaaatata
     3541 ccagtcttga tacttaagcc actttctgta gttattatgg tgatcttcac tctgatcact
     3601 tatggtctat tcagctggtt catgagatac aggttggccg cgatacagag gttccataat
     3661 cagatcgaga agaagctgga tgagctcgaa gagtcattca aagacaaagc gaagaacatt
     3721 aagaaggaaa aggaagaaca accatcgaaa tccataagtg aagagattct tgagaagtat
     3781 tcaagaagtc tggcttcttc tgaagaaagc gatgatagaa agctgattat cgcttctctc
     3841 ttcaaggatc ttggcaactt aaaatccgat caggcgagat cacttctcaa tgatcttctc
     3901 tcaaagcaat tactgacgga aaatgaattc aacagactaa caagactctt agtttagcgg
     3961 gaggaagaaa attgattcga tttagaagtg caacaataac cctgataatc ataaacgtaa
     4021 tcgtgtatct tcttgtcgcg tttatgggac tattcagagg tccgcaagga ataagctacc
     4081 gtgatcttat aacagtctat ggaggcgtaa gcagatttgc tctttcaaac ggtttgatat
     4141 atactcctct aactgctctc tttcttcacg gcaatttaat gcacattctt ttcaatatgt
     4201 gggcactttt ccagctcggt catgtagtcg aaggagtcta tggatcaaag tggtatcttc
     4261 tcttctattt cgttactgga ataagcggaa gtcttagcgc agcagccttt tctaatgcat
     4321 tcacaattgg ctcgagtagc gcgatttttg gcctggttgg aatcctgttc actcttggac
     4381 tgaaaaaaga cactccggta gttctgcgtt ctattactgg atactctctg ctaccgatta
     4441 tcctgattaa cctctttctt ggtttcagca ttcccggaat cagtaacgct gctcacatag
     4501 gaggtcttgt tgtcggagct ataataggct ggtttgccaa gccggcatat gcaaggtctg
     4561 ctagatctag aagagtctac acaagggtaa aggaaaagac tccagaagaa gcctcgcgag
     4621 atattttagt caagtatata cctgtcctta acggcatgaa agataataga tctgaggaga
     4681 ggactattag aattgctcag ctcaggagcg aactgagcag tctcaaggac caggagatag
     4741 gatccaaagt tctctgggag ctgtataaaa gggatcttct ctctcaagag gagttcgaaa
     4801 gacttagaag gttcctgtga attaaaaaga gcccctattc ggggctcttt ttattccatg
     4861 aagtctcgtt ctataggcat ttcagcatct atgaagtctt ccacgaacag aactctcgga
     4921 gcatcaatac cattgaaatt cgtagcatac tcagttgtgt aggcacctgc attaatgaag
     4981 tacaatatgt ctccgtagcc tatgtttttc ggtaggtcta tctcgtggta aatagtatct
     5041 accgaatcac atgtgggtcc tgccagatga aaggaaatct tagtatcgtc aacttttcct
     5101 tcggtcagaa cttcatacct gaaaccttct attgtctctg ttagaccgtg aaagacaccg
     5161 gcgtcgatat atacccagtc ttcactgccc ttcttgctgc gaagaatcac ctgactgacg
     5221 agaattgccg aatttccaac catggacctg cctggttcaa tgaagagctc aagatcagga
     5281 acaaaagaca tgtatttgtc cagtgctctg tttattatgt cgccataaga ctttacagac
     5341 ttgatttctc ttacatgctt tacgggcatt cctccgccaa gatttaacat gcgcaggtta
     5401 atattcttgg cacgaagagt cttgaaaacc tccgaagcat cccttattgc atcgtcccag
     5461 ctatttacgt tgtagttttg agaaccaaca tggaagctaa caccgtaagc gtcgaggcca
     5521 agctgatcgg catattccat gatagagatc acatgaccga catccgttcc aaactttctc
     5581 gaaagcggcc aatcacaatc tccaccgctg gttgcgattc tcccgtagac tttacttccg
     5641 ggggcgtgag ccgctatttt ttctacttcc atttccgagt cgacagcata atagtctatt
     5701 cctacggaat aagcgtatgc gatgtcttcg actttcttaa tggtgtttcc aaaactcatt
     5761 ctttcggggc ttatacccaa tgccagaagc ttctcaatct caccgcgtga agctacatcg
     5821 aaattgctgc cgagatcccg aagagtctcg ataatcctgg ggtgtgaatt cgctttaacc
     5881 gcgtaaaaca cctggacatt tctaacatgg cttacaatgt cgaaataatt gtttctaacg
     5941 taatccatat ccattatcaa gaacggtgtc tttatttctc ttgcggcctt cctaacctca
     6001 ggggtaatgt gcactacggc atccctccta taaaataatc aagttattgt agcatcgaaa
     6061 ctatgaagga atgccaaaga tacaatgtca tttatagttt aataaagtta caatactatg
     6121 caccccaata ttagccattt tcagcttgca atcaggagtc cacatacttt ttcgagaaat
     6181 tcctcatcta ggcggtcgaa actattctgc aaatgactgt caatatccag ttcacctatt
     6241 acctttccgt caactattaa aggtagcacg atctcggctt tcgtctttga actgcaagag
     6301 agatagttgt tctcctttga tacatccggg accaggaaaa ccttcttagt ttctgcggct
     6361 tgtccgcaaa ttccacttcc aaaggcgatt ttcacatgtt ccgtgggttc tccgacgtaa
     6421 ggccctaaaa caagggaact ctcttcattg acgagataaa agccagtcca gttgtagtaa
     6481 tcaacatact tgtccaggag cttagagatc attgccatct tttctttgaa agaacggcct
     6541 ttgatttctc tctcaatcac atcaagtatc aagtcaaatc tttcggatcg tttcccgctg
     6601 tccatagtat gacgcacgtt tacccttgaa tactcaagga aatccttcag ggccgccaat
     6661 atgacgcttg agaagtcttt gatgctccct tctttccagc aaaacaggag atctatcatc
     6721 actacatttc ctctggatgt tgggactatg agatgttgct gtcttccaag ggcacccagt
     6781 atgtggatca caaaatcctc tctttcaaga tgaagctggg gagacatctt gccgagctct
     6841 ttcagaactc tcgatttctc aataggtccc tgaatctccc agtagtgaga cagcctgtca
     6901 atctctctcc tttcgagttt gtctagtgac agagagagtt cttcttcgtc aaggttgttc
     6961 ttgaacatgt actcctctag aactctagga tgcttctctc tataacttgt ccagaaatcg
     7021 aaccatctct cctttggata gctcaaaatg ccgaagtaat cataagtgaa atccctgaaa
     7081 atgctcctca tctactacct tccccctact ccaagcaaca cataagcatc atacttagct
     7141 ggatagaatc tattctttga cagggaatct atcaacgcat aataataatc aagccctccg
     7201 aagctggaag tcaacataac aaagggtctg tgatagacat gcgcctttga gaagagctct
     7261 ctaatttctg agtctttcga atttatgaac agatatgtat cgcgcttttc aatgcctgaa
     7321 atattcggta caaaacccgt ggcatctact ttcactcctg cctgagcaag ccactgactt
     7381 tttattgttt ccgcgaaatc tccaaatctt tcctcatagt cgggaatatt gtttatcacc
     7441 agaatcttcg gcgacctgct ttcgatttcg actgctttcg gttcatcgct gccgggaccg
     7501 gttggaacta tctgaccctc atcatcgatg tagtaatcta tctgtgtgaa acttacggac
     7561 ctcttacctg tgaagaaacc aacggcgaag tcagtgagag tcttcttgtc tatgctgata
     7621 tctgtctttt caagcacgaa atctatcatt tctatcatct tcgacggccc agcacttaca
     7681 agatttcgga tgagctgttc caacactctc ttctgtctat cgatccgacc aagatcaccc
     7741 gaatcagagc ttctgtagcg aaggaacttc agcaggtcct caccttcgag aaaataaatg
     7801 ccaggttcga aatggatgtg aagtccctgc tggaaatcgt cgtgatgcat cggcgtggta
     7861 acttcaacac gaataggacc ggtgaacgtc gttatttcgg tcacagtatt gaaatcaaca
     7921 acaacggaac ccgttatcgt tctcccggta agcttttcca cttcctcgat aagcgcttct
     7981 atcccaaagg agttgtacag ggagttgatc tttctctttt cagatctgta agtaactaca
     8041 gtgtctctgg ggatgctctt taacaagaga cctccggaag tccgaaaata cacaatcgag
     8101 atataatctg ttcttccacc aactgcgtca cttgagtttc caccggaatc tactcctacg
     8161 accaggaagc tctctgccgc aagatctttt gccgagaaag agtaaaaagc gtccctgaaa
     8221 atgagaaaag tcagaaagaa aaagagaaaa acttgcagca ccgtcaacgc cacaagaaat
     8281 agatgaacag atttcattag tctgctaatg ccttttttac tgtctccagt gaatcgggtt
     8341 taagatacat tcgtgaaacc gtttcgcctc caactcccac tgatatcttc atcggaaact
     8401 cagttaaggt ttcgagctta taggtcatcc tcgatgaatt tcccaggcga cgaagtagaa
     8461 cagcgatccc ttcagaagtc aatatggaag cgacgtcgta ctccttaacg gtttgaacga
     8521 aatcatttat cttccagtaa tcaaataact taaaccctcg cagagccatg gcatcgatga
     8581 gttcgacagg attcgaagca ttgaccccga gttttgaaga aagagcatct atcaagtcgg
     8641 gggtgaaatt gacgaaataa gagaaatcac ctgaagtacc aagccaactc tgaactgctt
     8701 caacggcatc tgctccaagt tgcggatcca gaacctcttt ggagttctga atagtggcga
     8761 aaatcgggaa gattacaagc gatgttctgc ccaccgtcgt agtccttaca tagtaataga
     8821 gaggattatc cgcagactgc cagtagaaga gataatctac atactctgcc ttcatctcat
     8881 cgctgctgtt gaccccattt actatgaaat acacatagcc tgcacctgct acaacgacaa
     8941 gtacaatcac caaaatcaac cacaatagtt ttggaccacg tcgtctcttg ggcttaatta
     9001 aagacatgac tggcacctcc atgtaattca ttccacgtcc acacggtctc aggaagcaag
     9061 agactgtccg actcaacggc ccatgttatt ttcaatctta tcacgtcaac aaatgttttt
     9121 tccagatcca cttctgcagt ttcacgaata tgaagggctt cggaaaagtc tcttccttct
     9181 tcagcaatat ccgaaatcat caaaatcttt cccgtagtac tcatgcccgg aatcccgctt
     9241 acatgccagt agacagcctg aaagacatca ccgtcaactc cgtattcctc tttgagataa
     9301 agggcggcaa cttttccgtg aagaagaaca ggtgcaaccc tctccatttc agtcatatca
     9361 atcccaaagg aaatagctag tctcattagt tcgtcttccg cctggtctct aaacatatca
     9421 tgtgaaacgg cagcgagacc aactcctgag gaatcgatac catgaatgct gcttaatctc
     9481 tttgcgagtc tttccactct caaaatatgc tcgaatcttt ccggagagca catcgacttt
     9541 gccttggcgc gaacttctct agttaccgta tcgtactctg taatcaactt taacctctgc
     9601 aatttgatcg ggttcaaccg ttacaactgc cgaccatttt ccttcagctt ttacgtattc
     9661 gaatttttcg ttattttcca tattgatttg gtctacattt tgtccaagat agtcctcgat
     9721 caccaccgcc gacgtttctt cagacagatt tctcacgtaa tatgtatttc taatgtatct
     9781 gtacgtgcca tcagtcttca ttgtctccga aatcttcttt ccagcaattt caatagattt
     9841 tgcaacgctt tccagtaaaa tgttctctcc ttcagctatg ttcccaattc ccgccgagcc
     9901 caagtaaacc gaatcgctcc agatatctat gataccagat ggaagatcta gcgggacctc
     9961 tttgaggcga acatcaatat cgacacccgt atagttgctc gctccccagt tggccctgaa
    10021 cactagcctc ttttctcctt caacaaacct cgatagaaat gatatgtaat tgatcccacg
    10081 gctcagcggg acagaaatct cgtaaagcct tgtttcagcg ccagaagtag ttggggatat
    10141 tgagtaggat gcattagcca ttttgcttga actctctcta gcagagtagc caaattcagg
    10201 attcgaagat acaagtgtaa ttgttgacgg tggctcaacg cctaagtcaa caatcatcag
    10261 accagtcatt gaaaaatcgt ccaccgatag ggagtacctt gctgtccagt tgttattcgt
    10321 cgtttggtag gcaaacctcg cgtgcccgct gtagtcttct tgagtctcta gcaaaaatcg
    10381 tcctgctgga gtgtactcac cagtgaaaac aggtgagccc tttggattga caagtatttc
    10441 tccatatgaa tttctgaata cgggaaagtc agaaacaact tctagcattt ccgatcttcc
    10501 atcgtcaaag aggaatttga tccatctccc tatgtgcttc tcatataggg actttaggct
    10561 atcggcagga acatatctta tatgtgttct ctcaggagca ccgaacactg aaaggctctc
    10621 gggtacgata tcagctccat acggcaattc tatgattgcg gtcccattct cgaatacgac
    10681 ttctccggaa tagatagaaa acccgctgaa gatatacaac tgctgagaaa tagaaataga
    10741 tgctaaaaac accagcatta acgagactag aattttcttc attcttcacc tccatttatt
    10801 accagatctc tccgtcctct cctgagactt cgagaccgtt ccagattatt cgctgccaaa
    10861 ccgattgaaa gagcgaatcg agttcgcccc tttcgtttgc caccatgcag acagtgattt
    10921 cgctctcttg ttttgaatca tgtttccccg tctcaacgac tgaagcattg aatttccttc
    10981 gaaggtcatt taggacaggc ttgattacgg ctcgtttctc ctttaaggaa cttattccat
    11041 acaatctcaa aagataagtc atatagccca cgtgcatttt ttctcggctc ctcctaactc
    11101 tcaatcctat gacatccact ggtcaactaa cgttcaaaga aaagcggggg atttcccccg
    11161 ctcaagtatc gtttataatc tcacttctgc tgctcttcca gttctctctg cctttcaaga
    11221 atgatcttct gctggacatc gggcggagtc tcctgataac cactgaattt catggtaaag
    11281 tatccacgac cactcgtaat tgagctgagc ttattcgcaa agtctagcat ctctgcgaga
    11341 ggaacttggg cgactacctt tgaagtaccc ttgccctgag gttccattcc cattggtcta
    11401 cctcttctag aagttatttc acccattatg tctcccgtgg actcctcagg aacaaagacc
    11461 tcgacgtcca tcaaaggttc aagaatgaca gggtttgctt ctgccatccc gtttttaaaa
    11521 gcctgtcttg cagctatttg aaatgacata tctgaagagt caacgtcgtg gtatgatcca
    11581 tagaaaaggg ttacttttgc gtcgacaacg ggatatgaag cgagaacacc tttcttcata
    11641 gcttccacaa tgcccttctc gacagctggg atatagttct ttggaacaac acctccaacg
    11701 attttgtcta taaactcaaa acctgcgccg cgttcattgg gctcaatctt tatctgaacg
    11761 tgcccatact ggccatgccc accagtttgt tttttgtgct tgtactcggc ctcaacagtc
    11821 ttccttactg tttctctgta agcgatcttc ggctttccaa cttctacatc aacagagaat
    11881 agcttcttaa gcctctcgat cataatgtcc agatggactg aaccaagacc gctgataact
    11941 gtctcgttgg tctcaggatc attttcccac ttgaatgtgg gatcggattc tgccagtctg
    12001 gcaagacctc cacttatctt atctatgtct ccctttgact tcggctggat agactttgat
    12061 atcattggtt cgggcataac cggtaggtcc agcttcagct gcctatcctt gtgtgcaaca
    12121 gtgttaccgg cagcgctctt cttaagtttg ctaagcttca ctatgtctcc gaccgtcgcc
    12181 tcatctactt caatttcctt tgtaccctcc ggaagcataa cgtgacctac cttttcggag
    12241 gaatcctgat caacaacata gaaagagtct ccctgcttca aagttcctga aagcagtttc
    12301 ataaaagtga gcttaccaac aaagggatcg acaacagatt tgaaaatgta ggcaacaagt
    12361 ggttcttctt cagcaggaac cacatcaatc ttttctccgg agaggagcgt tgccgctttc
    12421 ggagaagctt ctgaaggccc cttgccaact gcactcacaa agtccaggag ctgtgaaatt
    12481 ccaatgttct ttccagctga tccaagaagg accggaatta cttcgttcgc aaggtaggct
    12541 ttcctgaggg cagtgatcag ttcctctgtc gagagttctt ctccttccag atacttcatc
    12601 atcagttcct cgtcgttctg aacaatatcc tcgatcattt tcgttctaga ctcttcggcc
    12661 gctgacttta gatcagcagg aatatcctct tccttgaaag cgccttcgtt ctcgtaaata
    12721 taggctttca tcttcaccaa atctacaatt ccccggaagt ccgcttctct cccgattgga
    12781 agctgtacaa ggaccggggt tctctcaaat gtatccgcca gatccgagag ggtttcttcg
    12841 taaccggctc tctccttatc aagcatattg aagaacgcaa taatcccctt cttgagctcc
    12901 ttggcaatcg aaccgaacct ttctgtctgt atctcaagcc cggcagttga atttattacc
    12961 gcaacaacat tctccgatgc aaaaatacca tttgctgtct cggcataaaa atccgacata
    13021 ccgggagtat cgatcacata gattctctta tcattgtggg gtaagctggt gactcccatt
    13081 gagaaacttg ctttcttctc tttttcgact tcttctgaat cagtcgccag gatgcctatt
    13141 cgatcaataa gctttgcgtt gaaaagcatc gcatcaacaa tttgagtttt acctgacccg
    13201 tggtgcccaa tgagtacaat atctcttttg gcatcgaccg gaatcttgtc catatctgac
    13261 cacctccaaa tctatttcaa tctatttacg cgacccggtt attattacgg gaagagaggg
    13321 aactcccgag gatggagaat tgattatttc gccattcaca gacatcaccg atgatttgcc
    13381 gccatcaaga cacattagag attcaaatcc cttgtcttta aagaattcta tcatctcttt
    13441 gaaagttaag ccatagctac tgtgctggta tccatcgata acaatgaata ctactcctcc
    13501 ggtttctttg atgccaacaa gcgttctcgg tgctctgaca tccagaagcg atgccgagta
    13561 gtagttctgc tcataatcag tgaccggtct cccattatga attatcatgg gtccaccctc
    13621 aactgctccg gtgatcttca cgccaaatga attcattagt tcaaaggaaa ccgatctgcc
    13681 tacaaaagca gtatctccaa ttttctgcaa agacgatggt gaaagaacca gcaaatcagt
    13741 atccctctcc acctttgatg aaaatccttc ctttactatc cttccatcct ttaccaccag
    13801 ataagtgaag tcatcgaact ctggaatact gttacggtat tcatcggtgt acagcactgc
    13861 ttcgcctctg tatggagaat taacacccgt aatctgaacg aaccgatcat cgaattttgc
    13921 gaggtacaat atgtccatcc ttgtaacata tgtttttcca tcttcagtga aatatatcgc
    13981 cggtcttccc aggcttggga gacctaagac ttttccgttt tgaacaagaa gaccaatggg
    14041 gaggttttga ttctggtcga agtagccgcc gtttatcata atctctgaac cactctctct
    14101 cagaacatca gttgcctttt cgaggccacc aatcccgttg tccgcaagtt gaacgccaaa
    14161 agagaagctg gagccgttca tttcaaggta agacatagta aatttctggc cacctgaata
    14221 agattcaaaa atattcaagc tgaaatccgt actcgtttcc tgctctatcg ttctccctat
    14281 gccgcttttt cccatgagag tgagggttct ccctttaatc tcatattcta cgtctggcca
    14341 gggtttctcc acggatatct ttacaaaccc ccttccagtg gccactgtca ctccttctgc
    14401 atccagaaaa ggatcgattt tcgccggctt caaggttaag gctagagaac cgcttccagt
    14461 ccaccagaca gtgaaaaaat ccgggagaac gtctctatcc agagtaattg aaacagattc
    14521 aggcctgttc acaatgcttt ttatcgttac aggctgcgca tagagtatca gtgcctcagt
    14581 ctcgctgaag aaagacttgt cggtaattgc ggcaagtagc tcggcattaa tgtagaccat
    14641 gccatcctta aagatcatcg agttgtcaaa ttcacttcca aattccacgg ttgccctttt
    14701 tgtctcgtga gatagagaaa caattattcc cgaagggaga atcattattg tctcttcctg
    14761 tcgaagtgat tgagagacaa ctactcctct tatcagctca ccgataatat caagttttac
    14821 aaagtccata gatcggtcag ttacaacgga gttggtaaag accctcgtct gccctctttc
    14881 atcaaggtat atcagattct ttgctgatga gaccaatgaa atgagaagaa aacaaataaa
    14941 gataatgcgt ttcatataat gcactcctta gatcacttcg taaactattg gaggagggga
    15001 tgttttctgc tctgaaatga caaagctatc aacaagcttc ttagcctcat tatcggcatt
    15061 tcttttgtcg tttgcataaa taactgcgat aacttcatct ttctcgaccc tatcaccaat
    15121 tctcttcaag attcttactc caaccgatgg gtcaataacg tcttccttac gttctcttcc
    15181 agcacccagc ctcatgcaga ttagtccaat agactccgca tcaactgatt gtacgtaccc
    15241 gcttacactt gtcctgactt cctcaacaat ttcggcctct ttgaaatgaa ctgcataact
    15301 tgcggaaaat ccttctggac ctccttgagc gtagacaaat tcatcgaatt tcctcagtgc
    15361 ctcgccggag agtatcagtc tatcgacagt cctcaatgcg tctgcctcgc ttttcatccc
    15421 gcccgctacg agcatctgac ccgcgattat tctacacaga gagctaaaat cctgtggacc
    15481 gcgtccttta agggtatcga tggcttcaac aacctcaagg gaatttccca ctgaaacgcc
    15541 cagcggctgg ttcatatcgc taattacagc cttcgtggct cttccgttct ttcttcctaa
    15601 atcaatcata gctcttgcaa gcgttttggc accatccaga tctttcatga aggctcctgt
    15661 accaatcttg acatcaagga gaattccatc cgagtctact gcgagtttct tgctcattat
    15721 gcttgacgca attagcgaga tctcatcaac agttgctgta agatctcgaa gagcataaag
    15781 tttcttgtcc gcaactgcaa cttgcgctgt ctgacctgcg agggctatac caatgcgctc
    15841 ggcttgcctt ttgaattgtt ctatgtccag tgaagtcaca aagcctggaa ttgattcaag
    15901 tttgtcgatt gtcccaccag tatgaccaag ccctcttcca gacaatttag caaaaactag
    15961 gccggcggct gctacaatag gtccaacaac aagagtaacc ttgtcgccaa ccccaccggt
    16021 agagtgttta tcgatttttc gacctttgat agacgaaaga tcaatccgat caccagactc
    16081 tatcataatt tcagtgagat ggaacctctc ttctgcatta aggtgattga agtaaacagc
    16141 cattaaccat gccgccattt gataatcagg cacaattcct tcaacgaaac ccattatcag
    16201 tgaatccagc tcttctctgg tgttgggaaa accatttctc ttcttcatta ttatgtcgta
    16261 tattctcatt caaacacctt cttaagcggt agcttgactt tcaagatcgt tcctgtaccg
    16321 tactcgctct caagggaaat cgtgcctccg tgcttctcaa caataaacct tgtaatcgcc
    16381 agtccgaggc cggtcccacc catttttctc gatcgagctt tgtctactct atagaagcgc
    16441 tcaaaaatgt gtttcaaaga gtcttccggt attccaactc cagtatcttc tacttctatc
    16501 attgcggagt tattctgggc ataggctctg agccagattt cttttggccc gtgttccttg
    16561 gcaaaggtgt atttcacggc gttgtctaca aggttcaata gaagctgtac aagtctgtca
    16621 aaatcaccct ccacaaaaat cccttcatct acgtcgacat ttatggagac attcttctca
    16681 ctcgcgagag gctcaacaat tctcattacg tagttgacca cctcccctaa ctccacatcc
    16741 tctttgctga atgatgcttc accggattca agtttttcca gatcgagcaa gtcgttaatt
    16801 aatctcgtca ttctggcaga ttcgttttca attatagaaa ggaatctgta gacagtctct
    16861 ttatcctcaa gatcgtcctc ggcaagtgtt tcagcgtatc cgtggattga tgtcagcggt
    16921 gtcctaagct cgtgggagac atttgcgacg aaatctcttc tcattgcctc aacttccttc
    16981 tccttcgtga tatcgcggag aaggatgaga tatctaactt cacttttaag agtgactctc
    17041 attatcttgc aatttcttac cgatcttctg ggatagtata tagttatctc ggactcttga
    17101 ctctcctgag ttctaaccga atcttcaaag agatcgccaa tgtagtaact gtctatccct
    17161 tcactgattc ttagacctac gagctgcttg ctagaaagtt cttgcgcagc tttgttggcg
    17221 tattcgatct ttccgtcctc cgacgctatc aagaaggcat cgcttaagtt gtcgaagatc
    17281 atgacaaggt tttcgatcct ggtatctaga agagcaatct cttctttcat acttgagatc
    17341 agctgttgaa ccttcttata tataaagata aactcggcgc ttctcggaac ttcgacgaga
    17401 tccgcaaagc gccttttagc ggcttctaat ctcttattct tttgattctc atgtctaaac
    17461 agaatcatga agagaaaagc tatcaccgca acagcagcga ggacagcata aagcaattaa
    17521 tcaatcttcc tttccaggat ctctgaactt atagcctttt cctctgactg tgattatata
    17581 cttcggagta gaagcgtctt cttcgatttt cgttctaagt cttctgatat gaacatcaac
    17641 agttcttgtg tcaccatagt aatcgtatcc ccagagttta tcaagaagca catccctgct
    17701 gaaaaccttt ccttcgttct ctgcaaggaa tcttagtagt tcaaactcaa gaggggtaag
    17761 gttaaccatt ctgttcctca ctcttacctc gtacttttct gtatcgattt cgagatctct
    17821 tgcgagtatc ttctttggcc tttcctcttt tgcctgcgca ctctgctgaa ttcttctaaa
    17881 taccgctctt attctagcca gcacttctcg aacactgaac ggtttggtga tgtagtcgtc
    17941 tgcaccaagc tctaacccca acaccttgtc aaattcttca ctcttcgcac tcaggaaaat
    18001 tacgggtgtc tgtctaaact tctctgtcgc tctaaggttt cttacaagtt cgaatccatc
    18061 cattcctgga agcataatgt cgacgataaa catgtctgtt tcgttgtctt cggcaatctt
    18121 caacgcttct tctgcatcat aagctttcag gacttcgtag ccctcctttt ttaaattgaa
    18181 agtaaggagc tccacgatcg aaggttcatc atcaacgacc agaatactct tctttgccat
    18241 gctctttcca ctccttccgt tccgaattca catcctctgc gtatttgttc agaataggtg
    18301 ttggatcagt tccacgctcg acaagttcaa gtaattctct tcctttttct ctatcgatgc
    18361 tggcaagggc aaactgcaac cgcgattctt taaggctctc aaaatcgagt ttgactccct
    18421 ccatcatcag ttccgatctt atttccctct ctaactttct gagaacgccg accgactcca
    18481 ttggcatatt ctcaaacggc gtgccgggtt tcggaatcag cgggttcaga ctaagtacta
    18541 catttgaata acccatctct agagcttgat gcactgtttt cgatatttct gtcctgtcat
    18601 cctgggtctc aaaaacagct ccatatatgt aatacatctt caccctgtga aaatcattat
    18661 tccttcctaa ctccagtgcc tcagtgatat cttcctcgct tatccccttt gcaaaggcat
    18721 tccttatctt ctggcttccc ccctccggag caattgtgaa agagctttgt ccggaaagct
    18781 tcagagactt catcaatgtg tccgatagag aatcaagtct gagagacgaa actgacagct
    18841 cataaccctt ttcaatacag tgttctgcta tctcatcaat atctggataa tctgtaaccg
    18901 tcgcggcaat cagtccaaat ctctttgtga atcccgcgac tgaatccatg agatcaatcg
    18961 tgtctttcaa ttctctgaac cttggccttc caaatttgta tcccgcgaca cagaacttac
    19021 attttcgaaa gcatcctcta ccaatctcta tgagatacct gtctccgaag accgaatccg
    19081 gactgataaa agcgctttca cccaggcaat tagaaaaaat cgtttcctgg tgttctgtct
    19141 cgagagtaga aaccatctcc tctttactca tgcgatgatc aatggcagag agttgctcga
    19201 ggaatttctc accaagatct cccctataaa ccacatctgc aatttcagat acaagagagt
    19261 cattgaagta ggttaaggct ccaccaataa ggatcaacgg atgaaacctg ttcctttttt
    19321 ccttcctaag gggtatcgag aacttattca atatttcaaa gacgttaagc agatcgagtt
    19381 caaaatgcac agaaaaagac caaatcacaa attcatctag cggcgttaat gaatcaaaag
    19441 aataatactt cgaaaaaccc tcatgaaaaa agaatctctc cgcccttaca ctacccagct
    19501 cgttgaactt agtcatcaat acatgagtcg atagacttgc gcttcccact ttatatgagt
    19561 tcgggtagat aattgctacc ttcacatctc caaaaccacg tatttcttta acaaatcgct
    19621 ctcttgatct gagtctggaa actatctcat actcgctcca gctttttctg tctctcggtt
    19681 tacgcaccaa atgctctcct aaagaagtca acagagatga acttcctgga gaattcaaaa
    19741 gcacccgctt cggttcctag ctgtacaacc gacccggaaa agtgcacttc cgcactatct
    19801 gaaacggata ctatcctgat gtttataacg agctcctctt ccatcaatat gggagagtag
    19861 tgttttacag atgtttcaga aaccacagaa accagtgaat ccgattcgaa gtcagatatc
    19921 agattgtggc cacaactgtg gacaatcttc atcagtccac tggtagacag gagatttagc
    19981 agttcaacat cgtaccgttc cgaccatctc aaagaaacgt cggtaggaat aacagtttgg
    20041 gtaaattcac tgttttccag cgatcttaac cgctctaaat ccagcatttt tcgccacctt
    20101 taatcctttg caatatctgg ctcttctgca gatgctcctg gagcaatgtc tatggtctca
    20161 agatcatcct tgtaatagtt accgccttcg agtccaagaa tatcggccag ttccttcccc
    20221 gaaatagtct ctttctgaag aaggtactct gccgcatcat caagcttttt tctatggtca
    20281 gaaaggactc tcttagcttt ttcataacaa gtgagaacaa tagtcttgac ttcggagtca
    20341 atttcgctag cgatctcctc cgagtaattc ttcatccgtg tgagttctct gcccaggaat
    20401 acttcttctt cctctttgcc ccaggcaatg ggtcccagtc tttcactcat tccaaactgt
    20461 gttaccatac ttctcgccat cgcgctggct ctttccagat cactagcagc tcctgtggtt
    20521 atttctccaa agacaagatc ttcggctgcc cttcctccaa gcgcctgcgc catattatcc
    20581 agaagctcag acttggttat gagataccta tcctccagcg gtagactctc cgtaaaaccc
    20641 agggtagaag tcccacgagg aattattgtt actttgtgaa ccgggaacgc ctttggaaga
    20701 attaagccga caaccgcatg gcccagttcg tgataggcaa gtattttttt ctctctatcg
    20761 tttatgattc tcgatctctt tgcaggaccg gccagaaccc tatcaatggc ttcctcaagt
    20821 tctgccattc caatcatctt tttctttttt ctggctgaga gaattgccgc ctcattgatc
    20881 aggttctcaa gatctgcacc gacaaatcca ggtgtcctcc ttgccaagag ctgaacatcg
    20941 acttcgggat ctatcggttt tccgcgcata tgtatcttga gaatctctga tctccctttc
    21001 aaatcaggag gatcaacaga tattttcttg tcgaatctcc caggtcttag caaagcttta
    21061 tcgagaatgt ctggacggtt tgttgcggct attactatga cacctgtttt agcatcaaat
    21121 ccatccatct ctactagaat ctggtttaaa gtctgttccc gctcgtcatg ccctccgcca
    21181 agccctgctc ctctatgtct tccaaccgca tcgatttcat cgatgaaaag aatcgaaggc
    21241 gctgaagact tcgcctgagt gaacagatct ctcactcttg ccgcaccgac tcctacgaag
    21301 agctcgacga agtccgaccc gctaatgaag aaaaacggaa ctcctgcttc accagctacg
    21361 gctcttgcaa gcaatgtctt tcccgttccg ggaggcccca caagcagaag accctttggc
    21421 attcttgcgc cagtgtctgc aaatatcgac ggatccttga gatagctgac aatgtctgca
    21481 agttcttgct gcgcctcatc aactcctgcc acatctttga aagtcaccct cttatcagtg
    21541 ttaacgtact tctttgcggg gctcttcgta aaagtgaagg cttgagaatt cctaccggac
    21601 attgatctca ttaggaagaa ccatatgaat atgattatcc ccagagggat caaagtaccg
    21661 aggagattca cccagaaaag agaatcgctt cctcgctcga acgttatgtt tgcgcctctt
    21721 tcagcaagca agtcaatgtg attctggaag gtttgtgtta caagcgtctg agatggaaca
    21781 taagtctcaa gagaccttct tgggttgtct cccgttatgt agacaacttt tccgctgtcg
    21841 taaacaacta tatcgattat gttattgaag tcatccagaa attttgtgta aggaactaca
    21901 atgcttgtct cagttggata caggcccctg agagcaatca gcaggaataa acctaaaaca
    21961 acgtaaaata caatcattcc aatcctgggc ttttgatcca cagaactacc tcctttcaag
    22021 gctaaaaact acattgttac gactggaggg gccaactcta cacaactcgc tcgtgacaac
    22081 acctggaatc cagaggatct ctccaaaagc gttttcaaga accattatca tactcgagaa
    22141 gcccttaaga cctttgtctg acaaaatatc taccactttc ttctctctct tcattccaaa
    22201 gggtataatc ttatccttgt tgttcatccc tctaatactc aacggaaaga taattcggga
    22261 atctgaacat atacttttga aagcaccatc aaattcaccg taattctcac ttgaaattcc
    22321 cgccttggat tcgactttaa tttgccagcc ttcagcctca cagacaaaag gaatggattc
    22381 tataaaactt ttttcatgcc catgaaactc aagggttcct ctgtaaaaaa acacatattt
    22441 gccaatcctg gaagctttca aattctcttt gaactcgact gtccaccgac cttcgtcttt
    22501 agagaagaga tcaaaaaagg ctaacgcacg ctgccgagaa ggagggtagt tgtcaaaact
    22561 catcatagac actatctttc taaccagctc aagaatgaga ggtttctcta tctctctcag
    22621 ttcagagagc ttagcgaaat atatcccatc tgccttccca aatctagaca ttagcgtttc
    22681 aaccctcgaa tcaattaacg agtatccatc atagatattt tcaaagaacc tccaaattgc
    22741 tttgttaagc gccgggttta tctttcttag ttctggtatg acccgcaaac gcaggaaatt
    22801 gcgctcaaaa ccatcctcag cgttggtttc atcctccata aatgtaacct tgttaattgt
    22861 aacataatct ataatacgtt gcatatcgct gaacagcagt ggccttatca aatcgccgtt
    22921 cgatgggcgc aacccaatta cgcctttcat gcctgtcccc ttcaccaatc tcaataaggt
    22981 attctccaca agatcatctc gattgtggcc aagagcaatg cggtcggctc ctatcataga
    23041 cttcacgtct ctcaaaaaac cgtatcttac ttcccttgca gccgattcag ttgattctcc
    23101 cttcttcgtt ctcatgtatt ccctcacatc gcgcttttca tggaaaaaat ctataaagcg
    23161 atcttcacag aactgctcaa tcattcttgc ctcttcttgg ggtccctctt cgcgaaggcc
    23221 atggtttagg ttagccgcga agagctctat ctttaatgtt gatttcaagt tgtttagaac
    23281 atttaagaga gccattgaat cctttccacc ggaaacggca acaagaattc tctgcccttc
    23341 gcgtacgaga tcccattctt ttacaaatcg aagaacctct aactcgaagt tatccatttt
    23401 tcccaccacc aatagtaatg cgcccgtaaa gggcgcatct ggagccaacg aagggacttg
    23461 aacccccaac ctgctcatta cgaatgagcc gctctaccgg ttgagctacg ttggcacctg
    23521 gtttcaattt tatatcactg cttttcctat gtcaatcttt caggaagcat aagagacgct
    23581 tcttcgctct cttattacag taacctttat aacaccggga tattccagtt cttcttcaat
    23641 cttctttgct atatcaatgg ccatcttttc agcaagaaga tcgtcaacct tttcaggttc
    23701 aacgatgact cgaatttccc tcccggcttg aatagcgtaa gccttctcta tgtgtttgaa
    23761 cccggtggca atattttcga gattctccag cctctttatg tacatatcta gcgactctct
    23821 tcttgctccg ggcctggatg ccgagactgc gtctgccgcg gctaccagaa ctgcctcagg
    23881 agttaaagct tcggtctcac catggtgata ctgaatcata ttcaccacgt gactcttttc
    23941 gccgtatctc tttgcgattt ctccgccgat tattgcgtgg gatccctcaa cttcgtgatc
    24001 aatcgccttc ccgatatcat gaagaattgc acctctcttc accttatcga cgttcagtcc
    24061 aagttccgag gccatcaacg ccgccaaatg tgctacctcg atcgaatgct gtaagacgtt
    24121 ctgcccgtaa ctggtcctga acttcagtct accgagaagt ttgacaagct ccggatgcat
    24181 agaaggaatt cccactttca ttaacgcttc ctgaccggct tcctttattt cattgtaaat
    24241 ctctttcttg gatttctcgt acatttcctc gattcttgcc gggtgaattc tgccatcttc
    24301 cacaagtcta tctagagtca tcttggctat tgctcttctc agaggattga aacaagatac
    24361 gactacgact tcaggtgtgt catcgattac aagatctgaa cctgttagct tttcgaaggc
    24421 cctgatattt ctaccctctc ttccaataat cctacctttc atttcatctg aagggaggct
    24481 aacagcgctt accgtcacat ctcctgtgta gtctgcagcg taacgctgca cagaattcac
    24541 gattacccat tttgcgtaac ggtttccctc ttcatcgtag tgatctttca attccttcag
    24601 cttttgggca agatcatgtt catatatctc ttcagctctc ttcagaacca tattcctcgc
    24661 ttcctccatc gaaagctcgg ccaaactgaa gagcttcttt tcagcttctt ctttcatttc
    24721 ttcaacccga gcgcgctcta aatctagttg cattctcgtc ttttcaatag cttcttcctt
    24781 tttgtcgatg ttgtcttctc ttctcgaaat cctgtcttcc cattgcttca gttcctctcg
    24841 atctctcttg aagtccttct cctgctcttc cctaagtcga tggagttctt cgcgcccttc
    24901 aatgagagcc tttttcttca gatgatctgc ctcttcctga gcccttttta tgatcgattc
    24961 tgaatcttgc ttcgccgatt tcagctgaag ttgcagctcc tccttcgcct tcttcacggc
    25021 agagggggtg aatataaaat aagccagcgc tatccctgct gcgaggccgg ctattatacc
    25081 aatcaaaaac atcatagttt ctcaacgctg gtcctttcaa tttcagtcga ggaaaccagc
    25141 tccctccctt catgatttct catcaatttc atcaattact tcgtctatta acgaagacga
    25201 aaatcctttt ctataaagct ttcttcgcat tttatcagca tctgttcttt ctgtactacc
    25261 aattgcaatt tttgcaatct cttttaagtc gcagttttcc aattccttct caattgaatc
    25321 agagataatc tcttccaaga tgcccagttt gaaaagctcc tgtctgattc tgaatgggcc
    25381 cttatgatga acgttcagtc catcgctcac ataaagactg gcaaagactc tgtcgtctac
    25441 tagtttctgc tcttcaagtt tttcaatgac ctcatcgact tcttcctgag aataaccctt
    25501 tttcagtagt tggtcttcta tctcctttct ggatctcatc ctatacttta tgtatctaac
    25561 tgcatccttc attggatcgg aaagcattat ccttcctctt ccttctcctc ttttttagct
    25621 tctagaagtg gcaaattgta ttttgctcta agtctgtttt ccatttcaag gatcaactcg
    25681 ggattctctt tcatgaattc cacaccgttt gactttccct gaccaagact tacctcatta
    25741 ttatcttccg agatgtaggt ataccaggcg cctttcctct ctatcatgtt ttctgagagt
    25801 gcaagattga agagctcgtt tgcatgttca ataccctgtc catagattat gtcgaccttc
    25861 gcttctcgga atggaggcgc aactttgttc tttacgactt ttatcgttgt ctcgtttcca
    25921 aggaccgtat ttccttctct tatcgcgctc cctcttttta tctcaagcct tactgaagag
    25981 taaaacttaa gagctactcc tccagtggtg gtttcagggt tcccgtaaac taccccaatt
    26041 ttcattcttg tttggttaat gaaaatgact atgcatttgg acttactaac atttccggaa
    26101 atctttctca atgcctgaga cataagacgg gcttggagtc cgacttgaag ttcacccata
    26161 gccccctcaa tctcagttct agggaccagg gcagcgacag aatctactac aattagatca
    26221 acggcatttg atcttataag accatcgacg atatcaaggg cctgttcccc atagtctggc
    26281 tgagaaataa gaagattgtc aacatctacg ccgaggttct gggcatagtg aatatccagc
    26341 gcatgttcag catcgataaa agcagctatt ccaccacgct tctgaacttc tgcaatcgca
    26401 tgcaaggcaa tggtcgtctt gccacttgac tccgcaccat agatctcaac aactctacct
    26461 ctcgggtatc ctccaaccgt taaagcgata tcaactgcaa gcgatccagt agggataact
    26521 tctatgtttt tgtgttcttc cttatcaccc atcaacatca ctgaaccctt accaaactgt
    26581 ttttcaatgt cctttattgc cctttcaagt gcgttcttct tatccttaga cagcatgttc
    26641 aatcagtcct ccttcaaaac tgcatgtgaa gaccttcctg tatgttgcgc cttccctcga
    26701 aaggatagat tcgaatattg taacttctcc aacagggaag attatgtgct cattttcaag
    26761 agtgcttaga aacttgcgcc acatgggcgg actaaacttc attcttccaa ctgttagatg
    26821 gggtctgaaa ttctcgtcga cgggaagact taacgcctca agcactactc ttgtttctct
    26881 ctggagattg acaagatttt ggttccgctc gattgcaacc cagattactt tcggcagatc
    26941 cttgtgcttg aaataaccta ttctacccgt ctccaaagta aaagaaggaa agcctcttaa
    27001 tctgcgagaa agcatcgacg cgagcaaagc aagtttgtcc aactcaatgt tgtcaagaaa
    27061 tgcaagcgtg acatgagcgt ttgctccagg cacccaggat gccttgaatc ccattcttct
    27121 aagcttttca gatataccgt ctatcattgc cgggactctc tcgccggtgt caactgcaat
    27181 aaaagagcgc aatttacttt cctcccttaa agatatcaag attcttaagt aggtaattgc
    27241 ccccacttaa cgccgttatg atagcaacaa ggatctcgaa ccaaaccatc acactcggag
    27301 cgggaaatcc ccagagcaaa ttagagtaaa gaagtatcaa gagcgcaatt tgcgccacgg
    27361 tcttggcttt gccaaatatg tccgcggcaa tcacttttcc cttacttgaa gcaaccattc
    27421 gaatccctga cacaagtgta tccctgaata tgaggataaa gaggaaccac agactgatcc
    27481 tggacagtcc cataaaccct aagaaaactg aagtaaccag tatcttatcg gagatctggt
    27541 caaggaattt tcctaaatcc gtaaccagtc cacgttttcg cgctattctg ccatcgaaga
    27601 aatctgtcaa agcagcgaca acgaaaacaa taaacgaaaa atagtgaccc acaacactcc
    27661 cgctcattag catcagcaat gcaggtatag taagcaacat tctggataaa gttaaaatat
    27721 taggcaagtt catccaagat aacaccttcc atgtcatgtt cataagaatc agtaattctc
    27781 gctttaacaa aaaccccatc ctcattatga cctttaagta ccaccactcc gtcaacgtct
    27841 ggcgcactgt tataggctct tccaagcgaa taacttgaac cgctttcttc aacaagaaca
    27901 gtcattatct gtcctttgaa ctgggaaagc cttccggctg aaatcgtgtc ctgttcttca
    27961 agcaacgaat tgagcatttc cttagccgcc tttctgccga tcgtcctctt ctggccgaat
    28021 gcctgagtcc cttcttcgtt ggaataaaca aagcctccaa gtctatcgaa ttttacactt
    28081 ctcacaaaat caagggtttc attgaatgtt gcttcacttt caccgggaaa accaatcaac
    28141 aaggttgttc tcagaatagc gtgaggagcc ttttgcctta ttccaaacag cagctcgcgc
    28201 aaccgatctg aatcctttat cctccccatc ttcttcaaga ttcttgtaga accgctttga
    28261 actggaatat caaaataatc tacgactttt gacagtgaga gaatcgaatc gacaagctcg
    28321 ttgtcaacgt gatcaggatg aagataaaga actcttatcc agaaatcccc atcaatattg
    28381 ttgagcctct caagaaggct cttgagatcc gttttctcat tcaagtcttt tccatattgt
    28441 gtacaatcct gagaaacgag atcgatctcc tttacccctt ttcttatgag agaaagagtc
    28501 tctttcacta tactatctgg atttcgactc accgacttcc ccttgaaatc aggaatagaa
    28561 caaaaggcgc agtttctgtt acagccatct cctatcttca cataagcaga atacttactg
    28621 ctgaccactc tactggaaaa gtcgtatact ccatccggct ccttcaaata gaagaactcc
    28681 ccattttcaa ttagatctgc aagagtaaat ggacttgtaa ctcctataag tccatctatc
    28741 tcagaaattt ccgattttag ttcatcaaaa tatcgctgca caagacaccc tacagctact
    28801 accttcaatc caggattctc ttgcttaaga gaaatgaatt tgaagatttc ctccacactc
    28861 tccttttttg catcgtcaat aaagccgcag gtatctataa ttatcaaatc cgcaactgaa
    28921 gcatcttcaa ctatctcgtg tcctctatta gccattatcc ccatgaagtt gtccatgtca
    28981 gcgatattct ttgagcatcc cagagaaaga actccgagct tcatagcctt tcctttctct
    29041 tgagaagata caactccctg attttcataa gctcttctat gtattcacga tctttgtaat
    29101 tgggataagt gtatggaaga ggtttcaaag agccatcgat gtacataagc gtaatttctg
    29161 cataaatgcc gtctccaaca taaacacgat ttgcccagtg cttcgtagag gccagcacga
    29221 actgcgcatg atgaatgtaa cccggatcca gatttacctt tcgattgcct tccaacgtga
    29281 aatgatcttc tatcgcattc gttaatcttt ttatcagaga aatctgttgt gggtgaataa
    29341 gccttttgaa ggagatcaat tttcccctca agccatatcc catttcatca ttgtaataag
    29401 atgtgtacct atcaaaatcc agagcctttg aaatgtagtc tgctggccca aatcttttct
    29461 caagaacagg ttgaatctcc tgcaatctgt aatctatata tgaaccgaaa acaaagatta
    29521 cgagattgac catctccgtc tttcgataat tccccatcac tttacactca ctttcacacg
    29581 cggaatactc acattgacta tctttccgcc acctcttact gtgtatcttc cggctacttc
    29641 ttgctcgaca attacttcgt catttcgccc gatacttagc atcaaactct ccccaggctg
    29701 aagaacgtat ccttcatcat ccaggagaat cctgatcggt gtagtattat cataaaaaat
    29761 tacctcaaat tcagccgacc gcgattcctt cacatatctg ataatgtaca aagtttcaac
    29821 aaaaaggaaa agagatatga tcagcattag tactaataca aattggcttt tcaaaacaac
    29881 tctcttttcc atagaatgtg ctccagtcta cattatgaac attctaaagg taattatagc
    29941 acgtagctgt ctcacatttt tgagtggctc aatcaaagat gaaccgacgg taaccatgat
    30001 ataattttcg caaagaaact cctggaggaa gataaataat ggacagtagg aagattccca
    30061 agcccacgat caagagactt gcaatttttc acagatgcct agagaatctg gtgatctctg
    30121 gggtgtcaag catatcttca aaagactttg ccgacaggct aggcatcaaa gccagtcaag
    30181 tcagaaaaga cctttcatat tttggcgagt ttggcaaaag gggtgtaggt tacaacaccg
    30241 agcttcttct tgacagcata agcgagatac tgggaatcag aaagaggtgg aatacctgta
    30301 tagttggaat gggaaatctg gggatggcgc tggccaacta tccaggcctt tccaaatcgg
    30361 gattcgttgc aaaggcactg ttcgacaaca atcctaacaa gataggaatg cccatgccaa
    30421 ataacctcat gattgagtcc acggccaaat tgaaagaaat agtcaaaagt aggaatattg
    30481 aaatcggtgt gattactgtt ccggcaagcg cggcgcagag caccgccgat ttgctaacgg
    30541 aggcaggtat aaaggtcata gtgaattttg cgccaataag actttcttta cctcaggaga
    30601 tcataaacga agagatagat atttcggctt ctttccggtc acttgctttt agcatgactt
    30661 atggatcttc aaagaaagga agaaagcatt aatggtcctc gtttcaataa ctggtggcag
    30721 cggggcagga aagacgtcgg tgacaaatat gatcttcaat catttcatgg agcgagctgc
    30781 ggtcctttct atggatgact attataagaa tctcgatcca ggcaccgatc caagacaatt
    30841 caactttgac tcccctaagg cctttgattt tgagcttttc gaagagcatc tcagatttgt
    30901 taaagagaac aaaagcatta tggttcctgt ctatagtatg gtgacctata ggcgtgagtc
    30961 tggaatatgt agaaagttca tacctaaacc gttgattatt gtcgaaggac ttttggtaat
    31021 gttcaagaaa gaaatgaggg atctcttcga tttttcgatc tacatagatg caccggctga
    31081 tgaaaggcta attagaagaa ttgaacggga tacaaaggaa cgagggagat caatagagag
    31141 cataatcgaa caatacagaa aatttgtcgc cccctctttc gagagtttca tagaaccgca
    31201 aaaatacttt tgtgatatag ttttacctga cggggccgca aatactaccg gtctcaatgt
    31261 gataatcaat gctattgaga atatgttaaa caagtgatcg ggctggaggt gtgaagtgcc
    31321 aaagagatta ctctcggtca ttgtgtttct ggcgatcgcc attctttcct tttcttttag
    31381 ccagaaagtt attatcgaag atttcaaggc ctacgtagaa gactacaata aggaagcgcc
    31441 aagtcttcca aaagttctga agctgattga agatctcgat tatttgtctg tctacagact
    31501 ttacaaactt caaatggttg gtagcataga gaagaaggaa agttcgacaa ctattgccgg
    31561 acttcttacc aggcatattg aagcattcga tgaaagtgag tttcgtgaca tcgacgacag
    31621 aatcgcctac agtgcctttc tcgcctgggt ccttactgat atttctggaa agccttttga
    31681 aatacatacc ctcaatgaaa tgccggctta ccttgaggtc ttcaatacat attcctcaag
    31741 agtacgtgaa atggccggag aagtctacaa agaatggatc gcctattctc taggcctaat
    31801 agaaaccaag cccggtttat ttcctgacga actcctggtg acggattcct tttcttccta
    31861 cagtattgaa gctgatgcgc catatgattc agaaaaagaa atactaagcc tttcaaacag
    31921 aagtgtaatt gatgcgctta attcagcaat ttcgaccata agcaacagag aatatagtgt
    31981 tagctcgctc gtcgatgagg aagtcaatag attagcagtt caaactgcga tggatctttc
    32041 acaggtaggt aatgcagaaa ttatgtcttc gggaagagag cttttcagac tttggcttct
    32101 tagatcaatt ggattgattg ataaagcccc attttatcct gaatccgttg agataagagt
    32161 taaggacatc ccgggatttg agattgattc catacctgaa gactcctact ttcaggattt
    32221 gatggacatt cttgaggaga atcaagaatt aagatctcaa atagtagaga agatccagat
    32281 gggagcccag cttttatctc taaaacagtt tactcctaca agtctaatag aaagcgacat
    32341 cgaaagcgaa gtaaggaaga tcgttccgat tcaggcaaac ataatgggag gaataaggaa
    32401 cgagctttcc aagagcctag tttcaagtat tgataaacgt atcgatctct ggtggttaag
    32461 aatattggcc tatgcactga ttgccatttt aggattcaca gttgttcctg ctatgagaaa
    32521 atatgttatt ggaataataa taattctgga aacgctttat atcttcttca tcggagagat
    32581 aaccacgggc atatttgatt tgtcgcttca ttcagttgtt gctctgccag cgtttgcttt
    32641 cgtgttcata cttgctgttg cagccgcatt cagtagaaga aaaaggagca ggatacttat
    32701 tctgaaactc cttctccttt tcttgatagc attactgcca ttcatgaacc ttctcaacga
    32761 acagcctgaa ctcctgatgg acaactttga aggattctat gattccgttt attattcaac
    32821 tttgaaaaat gacgtctttc ttgcgccgga atcgatgatt agccttcaaa caagagactt
    32881 gaactcactt gtttcaagtg aattgaatag cttcaagaga gtcttgagag tcattatccc
    32941 gaacaagatg aactctttct caacggctgc tgaaatgagt tttagtgtcg actcaaacgg
    33001 aaggctaaga gtggctgcac cggcattcag tgaatacatg tcaattgaaa atcagaatac
    33061 ttatgtctct gaattagaag gccttacaaa agatatagaa ggtttcattc gcgacagcga
    33121 aagaaatcga aaaggttacg aagctgcctt atcaacccta atagatactt cagaaagaat
    33181 tgttagtttt gcaggtagta gaatgagaga agatttttct aattcactgg aaacggagct
    33241 tcagtcgaag ccggaacttg aagtcgccct tgatgattac cgtgaaaaga tggcccctct
    33301 tctgaatgac gccccttctt cagttccagt taaggtttac agagttcccg aatttgcatc
    33361 gcttgtcgtg gctctgctct tgctctcaat aacgttgttc gttgtgcgaa aaccggttat
    33421 ctccttcatc aatcttgcag tgatcacagc ttatttttta atcgttctgc cgggtatcga
    33481 tacgctaaat ctctttgttc aaggcggatc tccgatgcta agaatatcta ttgaaccctc
    33541 agtaaacgct ttgtttttga cagtttttgc gggtataatt gcattatcag ttctttggat
    33601 cttactatct cataagaaag ggagtgttgt tgaatgaaac taaaactttt gttggtgttc
    33661 tcacttattc taatgtttgc tctcccggta ctggctatga aggtaatcat ggttacagac
    33721 gtcggaggac ttggtgacaa gtcttttaac gacggcacgt gggaaggaat agtaaaggcc
    33781 tctgattttc tcggactgga gagagaagta gtccagtcga aagagcaggc cgactacatt
    33841 ccgaatctgt ctaatgccgc caaggaagca gacatcgttt ttggagtagg cttcatgatg
    33901 gctgacgcac tttacaaggt tgcccctcag ttcccggata cttacttcat tgggatcgac
    33961 atcgatccga tcgagaacat gcccaacaac gtggcaactt atcttttcaa agaacaggaa
    34021 ggagctttcc tagttggtta cgttgttgca gctatgacga aaacaaacat ggtaggtttt
    34081 gtcggtggtc tcccaattcc tccggtcgaa aggttccgct atggctatga agctggaatc
    34141 agagtttacg aagaactcca tggaaagacc ataagcatac ttcagggata tacgatggac
    34201 ttcaacgatc cgaagaaggg aaaggacctt gccatcgctc agttcgcaga aggcgcggat
    34261 attgtcttcc atgccgcagg ggcctgtgga aatggagtca tcgaggccgc agctgagaag
    34321 ggtgagggct tcttcgcagt tggtgtcgat gtcgatcagg attacatggc tcctggaaga
    34381 gttcttacaa gctcagttaa gagagtcgac atggcttctt atcaggccgt catgagtatt
    34441 gctctgggaa cattcgaacc tggagcaaag acactgggta taaaggatga aggcgtggga
    34501 ataagcccta tgacctatac caaggatata gtaggaccga ctatcctttc tcaagtcgag
    34561 ttcctgaagg gtctaataaa agccggaacg ctagttgttc ctgatactca ggaaaaactt
    34621 gacgctttcg ttgtcccaga aataaccctt ccttaatgga agctcttcac acaaccgtgt
    34681 gatatcaaag atcgggagag taagctctcc cgatctttcg tatcatgtac gatggaggtg
    34741 ggcgtgtgga tttgaattac gttgaaaacc tcgatctctc tggtattgca gtagccatga
    34801 aaggcatagt gaagcaattc ccttctgttt tggccaatga tcatgtgaat tttgaagtca
    34861 ggaagggcga gattcacgca cttgtcggtg aaaacggtgc cgggaaaacc accctgatga
    34921 accagcttta tggactttat acccctgatg aaggggaggt ttttattaac ggtagaaaga
    34981 caataatcaa cgggccgaaa gatgccatag atatgggaat aggaatggtt caccagcact
    35041 tcatgcttgt agacaatcaa acggtagccg aaaatgtcgt cttaggatct gaacctaggc
    35101 gaggaccctt gaagctattc atggactccg gcagggcaag aaagatagta atagagttct
    35161 caaaaaaata cggcctggcc gttgatgtcg atgcatatat cgaagacatt ccagttggca
    35221 tgcaacaaag agttgagatc cttaagatcc tttatagggg cgcagagatc ttgattttcg
    35281 atgaaccaac tgcggttctt acccctcaag aaacagagga acttttcaaa atactccgtg
    35341 ttcttaagga aaacggcaag acaatagtct tcattactca caaattgaat gaggtaatgg
    35401 ctattactga cagagttacg gtcatgagat tgggaaaggt aaccggtcaa gtcaatacta
    35461 aagacactaa tccaagacaa ctcgccaata tgatggtcgg cagggaggtc ctcctcagag
    35521 tcgaaaaaga agagaaaaag gcaggggaga ccgttttgga gataaaagat ctccacgtaa
    35581 aggataacag aaatctcgac accgtaaatg gcgtatcctt cgtagttcgt cggggcgaga
    35641 tagttggtat cgccggcgtc gcgggaaatg gccagacgga actagttgaa gcaattactg
    35701 gacttagaaa ggttgaaaag ggaagaatct tcctcaatgg agaagatctg acaaatcata
    35761 cagctcttga gattagagag gccggaatga cacacatccc agaagatcga cacaaaaggg
    35821 gcatgataat gcaatatccc gtctattata acctcatact ggggaagcag gatgaagaac
    35881 cattttccca agaggggttc ataaatttga aagctgtcaa ggccttcgca agtgatgttg
    35941 ttgagacttt tgatataagg ccaaagaata taaacatgat tgctggaaat ctctccggtg
    36001 ggaatcaaca gaaggttgta gtgggccgcg agatcagcat agtacccatt gaacccaaag
    36061 tggttgtagt ctctcagcca acaagagggc ttgatattgg tgcgatagag ttcattcata
    36121 agacgctgat atctatgcgg gataagggaa tcgcattgct gctgatttcg atggaactcg
    36181 atgagatctt ttctctttca gacagaatac ttgtcttcta cgaaggtcag ataatgggag
    36241 aagtggctcc gcatgaagtt aatagagagg agattggcct tatgatggcg ggccataaga
    36301 agaccgaagt cgtcagggaa cggagtgatg aatcatgaac acgggcaaaa tatatgcttt
    36361 actagttcct atcgtctccg ttttgatagc tctcctaatc gcctccataa ttatcatcat
    36421 gatcggaaag aatccagtga cagcttacgc gataatgctt gatggagcct ttggcagcca
    36481 ggaagctatc gcagacacaa taatgaagat gacgcctctg atccttaccg gtctcgccgt
    36541 aggttttgga ttcagagcag gtgtctttaa cataggtgct gaaggtcaga tgacaatggg
    36601 cgctcttata ggcgcagtgg tcgctatgaa catcggtgga attccgtcag ttatagcgat
    36661 ccctctttct attctcgcgg gaatgcttgc aggagctttt tgggcttcaa ttgccggatt
    36721 tctcaaagcc ttcaccggtg cccacgaagt aatctcaacg attatgctga actggattgc
    36781 ttctaacttg gcatcttata tggtaactgg gcctctcgcc gtgggctccg gaactccaaa
    36841 gagtcctgag attgcggagg cgtcgaaact tcctgtgatc ttgaaggttc aagcgactga
    36901 actcagcatc ggaattttca ttgctgttgc tgttgcaata atcatgtata tcttgattga
    36961 aaagactaca acaggctata agctgaaggc agtcggattc aaccctcatg ctgcagagta
    37021 tggaggtata agcatcagga agagcataat tcttactatg gcgataagtg gcgctctggc
    37081 tggaatggca ggaattgtcg atcttattgg tgttccacct catcgattcg ttggagaact
    37141 cactggggga agaggctttg atggaataac gattgcgctt ataggaagaa acaacccgat
    37201 tggaataatc ttcgctgctc ttcttatagc tgccctaaga accggttcaa atgcgatgca
    37261 aattcgagct cagattccaa acgacattgt ttcgataatt caaggcatag tgatattcct
    37321 cgtggcagcc gaaagaatag tatcaacgct gatttcctgg aagcgaaaga aaggagtgac
    37381 tgcaatatga gctggatcga agcgatcttt gcaattctgg tcaacccctt gttctataaa
    37441 ctaacgctgc tctctgcgac tcctttaatc tttgctgctc tcggaggaac ttatagtgaa
    37501 atcacgggag ttacaaacat tgcgctcgaa ggcatcatgc taatgggagc ctttacttcg
    37561 atagttttca ctctaataac cggaaatgcc tggctcgggg ttctgatggc agtaattatc
    37621 ggtactggct ttacttggtt tcatgcgtgg gcaagtataa ggtggtctgc aaaccagatt
    37681 gtaagtgcta ccgctcttgt aataattgca caaggcacaa cttcattctt gatgattcct
    37741 atctttggta acgagggtca aacagacttc attggccgcg tgccatatct tgatgctggc
    37801 tggctaagtg agattccctt tatcggagat atttttggtt ctatgagtcc ctttacttac
    37861 ctagccatta tcgctgtaat cgccagctgg ttcttgatgt acaagactcc ccttgggctt
    37921 agaatgagag ctgtcggaga aaaccccgaa gctgccgata ctcttggaat aaatgttttc
    37981 aagatcagat atttcggtgt tcttatgagt ggcgtttttg caagcctagc cggagctttt
    38041 ctttcagtcg gagagcttgg aagattcgtc gaaaatatga ttcacggaag agggtttatt
    38101 gcgcttgccg cgatgatact gggtaactgg aatcccgttg gagccatgtg ggccgcaatt
    38161 ctctttggcg ctgcggaagc aatgaatctt cagctacaga gcagctctat agtctctgtt
    38221 tcgtccagcg taaaacctct tttcaatatg cttccgtttg tggttacgct gatagtagtc
    38281 ggaggattca tcgggaagac acggtctcct gcagcttccg gtacacctta tgaaaaggat
    38341 tcatgacaaa taaaaagagg ccttgattgg cctctttgtc tatatagagt tgattgctcg
    38401 ctggagcctg ttaagagtct tgttccttcc caagagaaaa ataatatcta ctaattcggg
    38461 cccctcttcg acagatgtga gtatttttct cagggtcata tagaaagcct ttctgtccgg
    38521 tttcagatct ttgagaatgc ctcttattgt accaattatt tcatcgttgc tccaggaatc
    38581 aagatactcg actctatctt tgaaagcttc caaagcttta taaacaccct tttcaacatc
    38641 gttggaagga gtgaaaaaag tcacctgagg atcttcgaaa tatagcctca tcttatccgg
    38701 tatttcggag agtgtttcca caccctttcg aacggaatca actgctcttt tcagccattt
    38761 tctattggcg acggcgtctt ctgcagtcat caatttcgat tgaatgataa aggggactgc
    38821 caaatcggtt attctttcaa gatcagattc gcgaatgtaa accccgttca tccacttggc
    38881 tttcgcctca tcaaatattg cagcgcttgt gttgaccctt tcaatggaga aactctctac
    38941 aatctcttcg tgattcagaa tctctttacc gtccgggtgt gaccagccaa gcagtgtaag
    39001 gaaattgaag aaggcctcag gcaaaaagcc cctttctctg aactgctcaa ccgaagtgtc
    39061 gccatgtctt ttacttagtt tctttccatc aggtccaagg atcatcgaaa catgggcaaa
    39121 ggagggtatt ggagctccaa aggcttcata gagtgcaagt tgccttagag tattggaaag
    39181 gtggtcgtct ccccgtatca cgtgcgagat ctccattgtg atgtcgtcca ccactacagc
    39241 gaagttatag ataggttgac cgttggagcg cataatcaca aaatctccaa ttgctccctc
    39301 cttaaagact acttcaccct ttatcaaatc tggaagtctg tactctttcc gaggcatctt
    39361 gaaaaaaacg acaggtctca accctttgga ttcgaactca gagattcttt catcggtgtt
    39421 gaaggcctca agcatctctt gatcatagtg tggcgccttc ccttctgata gcaatttgtc
    39481 atgcagttcc tctatctctt caggataggc atggacttca taagcctgtt ccctttggag
    39541 cagatccccg atcatttccc tgtaaatagg gcctctctca ctttgtctgt atgggccaaa
    39601 agcgccaccg acatctggac cctcatccca tgttatacca agccacagaa gcgactccat
    39661 tagctgatcc tcatattcct tagtggatct ggaaatatcg gtgtcctcga cacggagaac
    39721 aagctttcca ccgtagtgcc ttgcaaacaa gtagttgaac agcgcagttc tagctcctcc
    39781 aacatggagg tatccggttg ggcttggagc aaatctaact ctaatcatac ttatcacttt
    39841 cccttccagc aaaagcgagc aaaataccag aaaccgtctt gctatcggta atttgaccac
    39901 caattatcat ctttacaacc tctttagaat caaccagaac cggctcaaca aattccccct
    39961 catcgggaca cggctcctga gacttctcta cttcaccaac atatatgtga attatctcat
    40021 cggaaaagcc cggagaggtt tctattgtaa tcaatttgtg cagaaatctt ggtacgtagc
    40081 cagtttcctc aagcaactct cttctagcac aatcttcagg tctctcgccc gcatcaagct
    40141 ttccagccgg tatttcaagc atgtacttct gaaccggaaa tcggaactgc cttacaagaa
    40201 gcaactgctc actgacaaat gcgacaatcg cgacggcccc gggatgccta accacctctc
    40261 tgagagctag acttccatcc tgcaaaatta ctgtatgtct ttcaagcccc agaattcttc
    40321 ctgaaaaaat cctctcttct ccaactgaac gctccatttc acttctccat ctcaaaatgc
    40381 atcggcagca actcaaatgt agttgttctc ttaatattct cttctcctac taatatcact
    40441 tcaaagttcc caaattccac cataaactgc ctgcaggctc cacaaggaga agtcggctcc
    40501 ttcctgtcac ttactatggc tatacttctg aacttcctgt acccatttga aacagccgaa
    40561 acgatggccg acctctctgc gcacatagat agtccgtatg aactattttc aacattggtt
    40621 cccacgaaaa ctcggccttc ttcggtaagt agagccgcac caacgggaaa tttcgagtaa
    40681 ggggagtatg atcgctcctt agcctcaata gctttctgta ttagtgcctt ctcctccaat
    40741 ctgtctatca tcaacgctcc tcctgtctac aattagcctg attttctcaa ctttgttctt
    40801 tcccgcagca agaacttcga aagtgaatcc gtctacgata atcttttctc ccacttccgg
    40861 aaatctctca agaacttcca gcaggtaacc gccaattgtc tcgaactccg tttcaggaaa
    40921 tggctgttcc agttctctct caatatcgtt tatcggagtc attccatcaa ctatgtattc
    40981 tctctctccc aatctctcta tcattatctc ttctgactct tcatcatatt cgtccagtat
    41041 ttcacccgta agctcttcga tgacatcttc catggtaatg ataccggcag ttcctccata
    41101 ctcatctata acaactgcga gatgattctt cttctctttg aactccctaa gaagatcgtc
    41161 aaccttcttc gtctcaggaa caaaatatgg atacctcata atctcttcga cacggatcgt
    41221 ctggaggaca tcgttgtcct ttctgtcgag aatgaagttc agaagatctt tagcatagca
    41281 aacacccaca atcctatcga tattctccct gtaaatcgga tatctagagt atccttcgga
    41341 ttgcacgagt tccatcagat ctatcagcgg ttgttcttcc tcaagagcaa cgatttcgac
    41401 tctgggcgtc atgatctcct tcaccgatat atctttcagc tccagagttc tcttcataat
    41461 cattctctct tccgactgaa gaaccccttc cttatgcccc gcatctactg cagattttat
    41521 ttcgtcttca gttatgaaag gtgagaattc ggcctttttc ccaccgataa ttattataaa
    41581 gaaactcgaa atccgcaata ataaccacaa tattggtttc aagacaatcg tgattgccga
    41641 tataaaggtg attgttcttc tgaataaacg ctcggaattc tcacgcgcat agattttggg
    41701 tgtaatttct ccaaagatga gaatgagaaa ggtcataatg cccgtcacta acgctgcggc
    41761 aactccggcc gagttatctg gaagaacctg caatgcaagc aaggttgcaa gtgacgaaga
    41821 cagtatgttg acaacattgt tcatcacgag aattgctgtg agaactgaat tcgggttttg
    41881 aacaaaaaac ttcaccgact tgctgaatcg cttttctttg ctatcgacaa ggtcacggag
    41941 ttttaattta cccattgaca taagagcagt ttctgttcca gagaagacac ccgaaagata
    42001 aagaagaaag ccaagcaata acagattcaa taaaatcgat acaggatccc cgctactagt
    42061 agggtcttcc acttctgatt tgcacctcca gatcaataat gatacggtct gttgcccagg
    42121 atcgcaaatg cccgaaaaag ctgctccaat aggataagaa cagccatttc gtgagtaaag
    42181 gtcatccttg acaaagacag tttctgccag cccttagaaa ggacctcatt actaaatcct
    42241 aaaggtcctc ctacaaagaa agacagattg cttacaccta tattttgcca tttttcaagc
    42301 attcgtgcaa actcctgcga ggagtactgt tccccttggg catcaagaag cagaatcttg
    42361 tcctgcgaat ctgcagcttt caagtatctt ctgccctctc tctccttcac ttgctctctt
    42421 tctctggcgg tcgtcccacc aagcgggaaa tattctagat caactcttcc aaaagcacca
    42481 agccactttc tgtattgttc tacaccttca acaatgaaat ttgactttgg ttttcctgta
    42541 ccaacaatcc taatattcat cttacccttt ttccgctctt tattactccg cttacaatgt
    42601 tcctggaaaa gttgtatact atttcgctgt aatcaccggc ctttatcaga acaaggtcgg
    42661 catcgaatcc tgcctcgatc ctcccttttc tatcctcaag gcacaagact ttagcgccgt
    42721 tcacagtata cgcgattatt gcctcttcta cagacaaacc gcaacgggaa acagctagaa
    42781 agattatgaa aaagggatca taaatattgc atgaacctgg attgaagtcc gatgccagtg
    42841 caactatggc gccacggtca acgagctccc tcccctttgc aaaggcctcg ttaagaaaga
    42901 aggaagttcc cggcaggatc gttgcaaccg tattgctatt cgataagagc tccaaggtcc
    42961 catcgtcggc agaaatcaaa tggtctgccg aaagcgcccc caactcaact gccagtcttc
    43021 cgcctccaga cgcagaaagc tcatcggcgt gaagcttcaa cttgaatccc ttttcctttg
    43081 ctcgctttaa ataccaccta gactgattct cactaaagac accattttca cagaagatgt
    43141 cgactgtatc ggtatactct gacacgtcat cgagcatttc tgccatcgag tagagaaaac
    43201 tgtccgacga ttcatactcc tcggctacag catgcgcacc aagaaaagtc gggacaagat
    43261 caactggatg aatagaatca agtgttttca aaaccttcag ctgcttcaat tcattttctt
    43321 tgtcgagacc gtagccgctt tttccttcga tagtagtgac tccgagggaa agcatgcgat
    43381 cgagatgtct aagtgactct gcaaccaatt cagaagccga agccttcctc acctctttaa
    43441 ctgttgatct tatacccccg ccggtcttca ttatttccat gtagctctta ccggacagtc
    43501 gcatcaaaaa ctctttgctt ctgctaccta cgaagggaat atgagtgtga ctatctacaa
    43561 atccaggaat ggccacgagt ccagttccat ctatgtactc acgcgcctcc acgttttccc
    43621 taccaacata ggatatcttc cctccctcga ttgcgatatg catattgtgt cgtgtttcaa
    43681 gagaggacat ctccttgcct cttattgggc cggccttttt cggagaagct atctgcccaa
    43741 tattcaagat tgcaaggtca gcttttacca ttacctttcc ttgtccgcaa tgaagtccag
    43801 gacttttttc tcgaggatcg aagagcttgt gaacttttct aatctcaaat aatgatctgc
    43861 aacatccaca agcgcctgaa gtggcgttaa tccaactatc tccgatccga ctatgggaac
    43921 tccatgtctt tcagcctctg attttatcac ctcaaagacc ctgaaaagtg gagtctttct
    43981 atagttagtc atattcattg aaatctgcac tatatcccta tcctccagct tgaagccgag
    44041 agccttcaca tagcgaaatc ctccgcttat atgccttaca gacttcgcaa tcttgtttgc
    44101 aatttctatc ttattcgtgc caagattcac gttgaaggca atcaagtatt ccctacaacc
    44161 aacagccgtt actccagcag acgggtgaat ctctgagggg ccgaaatctg gcttccattg
    44221 gtcggaagca atctttgaag cgaagttctc gaattctccc ttccttatgt ttgaaaggct
    44281 aactcgttcg ggcgttgatg cagaatcctc atacaggtag acaggaattc gaagttcctc
    44341 tcctattctt ttcccaagtt tctttgaaag cgcaatgcat tcttcttttg taacattcat
    44401 tacaggaaca agaggaataa catcagtggc gcccattctt ggatgttcac ctgagtgatt
    44461 attaagatca atcagttctt ttgctttaag agtcatgtta aacaaagcag cttctacagc
    44521 ttccggttca ccggtaatag taattacaga acgattgtga tctggatcgc ttgagtaatc
    44581 aagaatccag acgccttcaa tcttcttggc ctcttctaca atccgttcaa caacgtcaag
    44641 tcttcttcct tcactgaaat ttgggactga ttcaataagt ctcatttgta ccccctcgca
    44701 ataaactact ctctctgctc tttatcatat tcgatctctt tcacagaata actatgtaca
    44761 atccctaact gaagcgcaaa taacatagac gacgacccgc cataactaac aaatggcaaa
    44821 gggattccag taacaggcag gagtccaaga ttcattccaa cattttcaac gacgtgaaat
    44881 gcaaaaactg cgaatatccc gctaagaact attctaccaa actcactgcc agtttttctt
    44941 gtgatggacc aaaccctcca taaaaggagc aggtacagaa caagcagaga cacagctccg
    45001 atgaaaccca actcctcacc aagaaccgaa aagatgaaat ccgtgtgatc tgcgggaaca
    45061 tatcccagtc tgttcattgt accctgaaga tatcctgtcc ctttaaggcc gcccgaacct
    45121 attgctctta ttgcctgaat cgtattgtat gcagctcctg aagcgtattc ttcaggatga
    45181 aggaagctca acaacctgtc tctttggtag ggttttagac caaacatgaa aacaatggga
    45241 gccaaaaagg caattagaac agttattgcg atcataagcc tgtcatatct cttgctaatc
    45301 aacgtcatga aataccatat gatcaataca atgattgtcg tgcccagatc gggttctaga
    45361 tatataagac ccacaaaaac aagagtgaaa aatataccca gcgaaaacaa tccaaacctg
    45421 tgcttagtgt ctcttgaata aacattccca aggaaaatta tcaaagagaa tttcgctagc
    45481 tctgacggtt gaaaacttgc aattcctagg tctatccacc ttcttgaagc ttcaccagaa
    45541 tctagataga gaactgccgc gagtgaaact gtcgaaagga tgattaatgg gaaagataag
    45601 cttttccata gctctttctt tacaacatac gcggtaaaga cgaacactgc gcaagaaagc
    45661 aggacccaga ccaattgtct tccaaacagg tactcctcac tttcgccgaa agtagcgctg
    45721 tacagaacga caagtccaaa tatcatcaaa gaaaccatga ttatcggaag taagaactca
    45781 actcttcgct tcattacact ctcctcagag gaataatcaa ttatataact tttctttatt
    45841 ctcgggcttg aggtctttga ttatcgatct aagagaaccc gtttcttccc cacttggact
    45901 ttcttggcga tggattaaaa tgtataatct aaatgaagat actaacttgc cccattgatt
    45961 ctttgaagaa ggtgcaaagt cctggtagca tcatttctaa aggagatgtg agattagaat
    46021 gaatgtgaga gaaatcgtcc aaatgaaggg tcaaagtaaa ctgtctgtga tctctgctta
    46081 cagctatttt gaagcgaagt ttgctgaaga ggcaggactg gacatgatca tagtagggga
    46141 ttctctcgga acgcttgtga tggggaaaag ggatactctc tcggtaacaa tggacgaaat
    46201 gatatggtct ttaaagtcag tcagaagggg agcggaaaat agttttatag ttgccgatat
    46261 gccctttggc tcttatcaat gttccaggga aaaagccgtt gaaaacgtgg tctccttttt
    46321 gagacatgga gccagcgctg tcaaaatcga aggtggtttc gagactgcaa accttataaa
    46381 acatttggtt gatagtggtt ttcctgtaat gggtcatatc gggataactc cacatcacgc
    46441 cagtttggtc ggagaatatc ctgtacaggg aagagacgaa aaaagcataa agagactaat
    46501 tgagtctgtc aacgaattag aggaggcggg cgtctttgca atacttcttg aaatggttgt
    46561 cgaagatgta gcgaaaaggc ttacggaaga tagttctgtc cccacaattg gtctgggttc
    46621 tggtcgatat tgtgacgggc agtttcttcg atggcatgat ctactcggca tcaacgacga
    46681 atcgagtcca agatacatta agagatttgc cgatcttaaa tccaatatca tatcagcact
    46741 tcgaagattt gatgaagagg tgaagtctgg cgtttttccc gaggagaaaa atgcttttga
    46801 aggggcagag gagatttgac gtttttctcc agattcgctc ggattttgag gaagaaatcc
    46861 cgaccatgat ggtaggcgaa aacattgtat cattctccaa aatagactca acaaatatgt
    46921 acgccaagaa taacctggac aaacttccct ctggaaccgt cgtgtgggct ctgtcacaaa
    46981 cttcgggtta tggaaggcac aatagtttct ggtactccaa tacaggtgga ttgtggttct
    47041 ctatagtctt caaaccgtcg ttgataaggg aacccaatga gtacacaaaa gtagtttcgg
    47101 tagctgtagc agaaacgatg atcaagatgg gtatcaattc agttggcata aagtggccaa
    47161 acgacgtgtt cattggaaaa aagaaagtgg caggtatttt gaccgaagcg atcttctcgg
    47221 ggatatcggt aaacgctata gtggtaggtg tcgggataaa tgtaaacaac gaactccctg
    47281 aagagattag gcagatcgcc ataagtctgg gaatggttat taacaaacac atatcactgg
    47341 aaatgcttct cgataaagtt cttaagagag tggagttcct tcgaaaaaac tatctaattc
    47401 gagggaaaaa cagataccta acacgcagat ggaagaagta tctagctttt gcggaaaaag
    47461 atgaaataac tgtatcccta cccagtggtg aaaaaatcac tggtataatt aatagcatca
    47521 ctccctcatc tttatgcctt gaaagagaag acggagagat cctcaagatc aactccgggg
    47581 agattctact ctagtttcct ccacgatctc tgactcgaat ccctctttta ctcttactac
    47641 aagtactctg ttgaaaaatc ttactgaaac aagatctccg ggctcaactt ctgaagaagg
    47701 ctttgctctg atcttgtttc tgaatacgta gcccttttca attgcctgct tagctaccac
    47761 tcttcgtttg ataattccgt tcattttcaa gaatttgtcg atgcgcaaaa ctgatctccc
    47821 atgagtatac acttgactaa cgataagaaa ataggtataa ttatgttatc gagatgttct
    47881 tgcccccgtc actccgcttg acgcaggggc ttcttttttt acttgatcac aattgtgctt
    47941 ctaatacccc ctatctctct tacatactgg ctgttcacta gaacaccact ctctatttcc
    48001 actcccatta atccagtaag tatgtaaccg gaatccaaaa aaagcctggc aaaaccttca
    48061 gaaccagaga acttcaacag ctcattataa acttctagat cagatactat cgcgccttcc
    48121 tgaagttctt tcgtgatctt cacctggtta cttgaagaaa cgtaaagcca cccgtcacta
    48181 acccaaatat actgatcttc tatagataaa ccattctcaa tgacgagaat cttgagatcc
    48241 ggatcggctt tttcaaggcg ggatttaaga tcatctaagg ttccactgta gcctgctctc
    48301 aaaacatagg aatatcctac tgagtctgtt gctcctgaag tagttccctc agtgcccatg
    48361 agacttgcga atatatcgtc aactgagatg ttggcatcta tcatgatttc tcccgtcatt
    48421 tcttcggaaa actctgtgat atcctgagta cctatcttga gtcctcccaa ctctaaagga
    48481 agaagtccca gctgttcgat atttaggtct gagagaaaag aaagatcatt tatctttgca
    48541 aaaccggaga cgagcggaag gtggtaaccg gcttccagtt caacaaccga agcaccttca
    48601 agtgcggaag agaacctctc tctggtctct tcagaaaggg ggatcgaaag agcctcagca
    48661 atcagaatcc cctcctcatt ccacagcgaa cccttctgaa caacatctcc caccgaagag
    48721 tagaagtaag caaacccagg cgttagacct tcagtgacca gagaaagatt aaatcccttt
    48781 tctgtaactg catactgctc aatatcacca atgaagaggt actcctgatc aacggcgata
    48841 ttcagttcag tctctgaggg tagaagctgc ggaagtatct tttcgatagc cgccttcaac
    48901 gcctttggat tctcagttgg tccgagcaca attacaagac gatccttacc ttctgaatct
    48961 acccagacag caagtccgag atctccatca aaagccttat atatgtcttt cggatctagg
    49021 ccattattgt aagccaacat ttcgacgtac tgcgctatca gttgctctat ggcaagctga
    49081 ttcaatacca tatccatgag aacggctttt ttggaaacgt tgtaattgtt ttctagatct
    49141 tcaactatta gtaccgtatg cgattgagct ggaagacgtc ccgacatggt gccgacgctt
    49201 gcaactgata aagctccaat aaccaggagt gcaagtacca taaatctcct catttattca
    49261 cctccaagat cgtactctga aatttgacga aaaaaccaaa ggaacagttc ttattgcgat
    49321 tctaccatct gaccgcttaa acaagcacaa caacgataac ggttctttct caagaagagc
    49381 aacagccgca gatcctactt gaagacggcg ttgacatttt gtatttattg atagagtata
    49441 ataaataaaa aggctctata gataatcaca gaaagtcaat agtgaagaga gggacgttac
    49501 ttgagattcc ttagacttgt taagaacgat cttcttctaa tagttaaatc gagggctttt
    49561 ctttttgtgc ttatcctttt gccagttatt ttggcggcca tatcttctat aacctccgaa
    49621 acgggaggtt attccgacct aagaatagga ataataaatg aggacaagac tttcataggt
    49681 ctcttttttg tgcagtacgc gacctctatg ctgaaaggag agaacatact tcagctatcg
    49741 aacagagagg agatagactc ggtgatcgac aaactcgacg gggtcttcat aattcccaga
    49801 ggatttgcga acaagcttct ctttcaggaa ccctctgaac tggttttcat tccaaaccca
    49861 ggatcaatgc aaactgcagt tgccgtctat caggttttga gcaatgttct gagagagttc
    49921 aaagcccttc cggttgtagc agatcccgaa ttcatgaaga ccgtggagat cgacccgaac
    49981 tacgtagcgc cctctctcac agttgaaggt atctccgagt ctaagctaag cttctcttca
    50041 cttctctttc ctatagttct ggttacaacg attatgttca taacatcaat aggagctgct
    50101 acgggtatct atgaagacag aaggaacggt gtgatagatc ttctccgact atcagatgtg
    50161 ggatcagtga aatatataat tgccaagacg gtcagttttt tgattttttc aactgcgcag
    50221 gttttgatct tcattacggc cggaagattg tttgggcttg aaattgcgtc gaatctgatt
    50281 ctttatgtgg tggtgttaga agtctacatt ctcctgttta catctatagg acttctcata
    50341 gggacaatac tgaattcttc aaggagtgct caattaacgt caataacgct tgtcacagcg
    50401 gtattgattc tgagcggaat tctgatacct cattcaatgt tccccgattg gctcgaatcg
    50461 ttcagtcgaa gtctcccaac cacctccctc cttgtcgtac tccagggaat atcaatgctt
    50521 gacttcactt ttcccatggt cgttttgccc actgcaatta atcttctgat agcaatcgca
    50581 ataataatcc taaccgggtt tgtcgcatct agggaggaca tctcgctagc agactaaatc
    50641 acggttaacg ttgactgacc gagtcaaaag agttataatg attgtagttg aaatactccc
    50701 aaagggagag ggcttgtttt gccctctatt ttttattatg gtggtgttgt ttgtggagga
    50761 cctgaagaaa gagcttttcg agctggcaaa tgaagtcgca agcaacctgg gctattcgat
    50821 ctatgatctc gggctaggaa gaagaggtaa gaaaagactt gttaagatca cgatcgataa
    50881 gctcgatgga tatgtttcta taagcgactg tgaatcattc tcggccgatt ttggtagagc
    50941 gcttgattca gcggacctcg tcccattttc ttacgatctt gttgtagagt ctcccggact
    51001 ggaacgcgct ctaagggatt tgaaggatta cgtaagattc acaggtgaga aggcaaaggt
    51061 gattctcaag gaacccattg aaggggtttc ggttttgatt gggaagatca tctcatgcga
    51121 aaacgacgtt gtcactgttg agagttccga gaacggtaaa gagatagcat tccacttttc
    51181 aaatgtcaag cgcgcaaact taaaactata atttggacgg tgaggtgttc acatgaatct
    51241 caatctcttg gaagcccttg atcaactgca agatgagaag aatatagaaa aggaagaggt
    51301 tatagacata ctggaaaaag ctctccagag tgcttataag aaaaacttcg cgtccgagag
    51361 cgatgtcgat gtcaggatcg acaggcttac aggcgacata gaagtctttg aaaaactact
    51421 ggtggtcgag gctgtggaaa acccctcttt agaagtcaag ctggaagaag ctttaaaact
    51481 tgattcttcg gcggagattg gctcaacagt tgagagaaaa ctcaacatta agaaattcaa
    51541 gaggatcgcc gctcaaacgg ctcgacaggt tcttattcaa aagatacgtg aaaaagaaaa
    51601 agagaacctc tttgataagt acgtcgatat gaagggaacg gtgacaactt ctgaggttct
    51661 tagagtcact gacgagtgga tcgatttgag gatagggaaa ctcgaaacga ggatcccaac
    51721 taaggaactc attcccgggg aacagccaag actgaattca cttatgaaag tctacgttgt
    51781 cgatgtcaca aaatctgtta gaggacctcg cttactcgtt acaagacgaa ccccggactt
    51841 cgttatagaa ctcttgaaac ttcaggttcc agaaatagca agtggtgacg taaccgtaaa
    51901 ggccatagcc agagaagagg gtattcgtac taaggtagcc gtattcagtc agaacacaaa
    51961 agtcgatcct gtaggggctt gcattggaga aagcgggaca agaatagccg aagtcctcag
    52021 ggagataaaa cccgaaaaag ttgatatatt aaggtggagc gacaatcctg cggagtttgt
    52081 tggaaactca atcgcacctg cttcagctct tgaagtgaag gttgcaaata tcgaaaacca
    52141 cgaggccacc gtctttgtct cgccaactca gctttcccta gcaataggaa aaggtggcca
    52201 aaatgcaaga cttgccgcaa agttgactgg ctggaagata gacattaagc cgattatgta
    52261 acgtagttcg gctttttgga ggctgtcatg agaaatgatc agctcaaggc agaactgaaa
    52321 aaaagaaatc aaagaatgac agctcaaagg gaattaattc tcagactttt ccttgaagct
    52381 ggcacagagc acttgagtgc ggatgagatc taccataagg ttcaggggaa gaacttcaga
    52441 ataagcaagg cgaccgttta caggacgatt gagctactcg ccgatataag gcttctcaga
    52501 aagatcgttt ttgaagatgg agtagtgagg tatgaactcg ttgaaaaggg tgagcaccac
    52561 catcatcacg taatttgcag caattgtggt gccgtcgtcg aatttcatct tgataacctc
    52621 gaagaacttg aagagcttgt gaagtcgagg accggttaca ccatagagga ccatcaactg
    52681 aaattttacg gcttgtgccc taaatgccag aatgcaatga aagacaagaa aagtacaagc
    52741 aacgaaataa aataagccgg gaaggcttcg aatcgcattg ccggaatcgc ttctccgata
    52801 gatgctgtaa cctttcccat tatgttcgac attgttctga ggcttcccga aataaaggaa
    52861 cttaccactt cgattcccag aaaatctgat accaatatgg ctatcatccc tatgtaagac
    52921 ggcattacga acatcggaac cattagaata gtcaaaggaa ttccgacaag agacaacgta
    52981 ccaaatgcgg tcaaagaaag aggaacaatt gccgcctggg caattgcacc aactagaaaa
    53041 gcttggtata taaggctctt cttttcgatt tctggcagga atatcagcag cgcagccgta
    53101 gcaaagaagc tcagctgaaa tgagatcgat gcaacaattg atggttgaac catcacaatt
    53161 atcattccag aaagtccaag gatattcagt ggatgctgac gataatcgat aatcctgaat
    53221 atcgaatagc ataaaagcat aagaaatgtt ctcaaggcgg agatagcagg tcctgtagac
    53281 aatgtgtata agaaaagcag agaaatcgaa gctattattc tggaattcct tccgaccatt
    53341 aggaaagaaa gtgcgctgtg aactagaaga tacatcagtc ctacatgcaa gcctgaaaca
    53401 gcgaacaaat gggcgattcc aagatcagat atcgttctcc tcaattcata ttctatgtgc
    53461 tttctgtcac cagtaaagac tgcagcggca atggtattgt caagttcgta ttttgaaagc
    53521 agaccagcaa tatgtttccc gggaagagag attatctcaa acctatcaat gcagccttct
    53581 acttccgatt cgattgtcaa caaaggatac gtgtcgctcc tcttaagcct gccagcagac
    53641 cacacgatct gcccgggaaa ggcaacggct tttctgtctg acaaatcgat atttacggcc
    53701 gggggaagat aaatccattc gccatcccgg agaagtctac cattacttgc ttcgatcctt
    53761 cccgttccac ctttcgaaac atagccgaca tactcaactt ttccaggatc gagcattttc
    53821 aaagtgaagg tttgtacaag gcagcccatc acaaagaaca agacccaggc aaacgggctg
    53881 acttgtttcg aagagataag cattacggaa agcgcgagaa gggctaaaga aagccaaccg
    53941 ctatccatcc agattgaaga aaatatgatt agctctccca gcatggtcgc cagaagatag
    54001 aacaaaaaag gaaaaggtgt acttcttcgc cggagcgcat cgtgcataac tagattctac
    54061 atcccttgca gtgctttgtg aacaacgtgt tcgatgattc acatgtgtta gaatattagc
    54121 gttgaatgtt cctggaggtt ttgaggtgag acagttttct tttggcgggc tgacgctggt
    54181 gggttactat ggatatgata accttggcga cgatcttctg ttgctttcat cactatccct
    54241 gatcgaagag atagaatttg aaggaccgat ttatctgccc gcctcaccga gcataaacca
    54301 catagcccac agatttccgt caaaactgga tgtaaggata attaagcgct tcagcataag
    54361 agagctccaa atctccatca agaactcgat tctaaccgta tttggagggg gaaatctcct
    54421 tcaggatcag acaagctgga ggagtttttt gtactattac gagattgcaa agtacactct
    54481 caagaaagac aaaccactgc tctttctttc ccaagggttt ggcccattaa gtaaaaccgc
    54541 aaacaggaag gcactgaaca agttgctcag tgatccgctt acttacggag tcatgagaga
    54601 cgccgtaagt tacaaacatt ttgcatcgat atcgaataaa gcaatcctcg gaacagacta
    54661 tggaccatac taccttcgaa aaaaaaggat aatacccgag aagcgtgaaa cgattgatgg
    54721 cttagcggta atagttctga aaaacggaac cagtgtagac gatgtactcc attctctcag
    54781 attgaataat ctatcacaag tttgcgccgt agggtttcat aatcaccacg atgagcaaaa
    54841 gagaagcgaa cttgaatcca aggcgcgttc aaacggattc aaagtaagaa aacctccaga
    54901 gaatcttgag ggaatgattg aggtttttaa caatgcagag ttaataatca cagagcgtct
    54961 tcatggagtg attctggcga tctcattggg cgttcctttc gtgtggaaga agaatactaa
    55021 gcttgataga tttgtgaggt ctctggacaa aaactgcaat ctctattttg aagaaagttg
    55081 tgagagcttg agcctatcaa tcaacagttc tttgaagaag ccggtgaatc ttaaggaaga
    55141 ctactggtct cgtcttgaaa acacggtgaa tgaatcaaaa gagttgctca agaaactcct
    55201 agcaagaagg tgattgaatg tggaaatatt tcttgccgac aagggtcttt gcaggtagac
    55261 aagtaatcgg ccaaaacggt gagttgctta aggaattcgg cacaaaagcg ttcatagtta
    55321 ctggaaaatc ctcagcaaag aaaagcggag cactcgacga agtactaagg atgctagaag
    55381 ctttagatat tccgagttca atattcgatg aggtagaaga gaatccttca ttctttacag
    55441 tcgaaaaagg aggaaggctt ctttcagaaa atgaatgtga ttttgtaatc gcaattggcg
    55501 gaggaagccc tctcgatgca ggtaaagcga tctctgcagt cggaatgaac ggatgcagtt
    55561 gcgaggacct atacagagga aagaaccttc cgtctcaccc aatagtcgcc atacctacta
    55621 cctcgggtac gggtagtgaa gttactcagt actcggttct tacaaatgcc gatggacaga
    55681 aaaaaggctt tgggatgcca tcgatctttc cagcgatatc ctttctcgat ccgagataca
    55741 ctgtgacaat gcctttagat gtaaccattg ctaccgcact tgacgcatta tcccattctt
    55801 tggaaggcga agtcgttaac aagggacaaa atcccctgct aaaaactctt tcaagagaag
    55861 caaacaggct cattaaagat tcgctcgaca gaatactgct ggatgaggat gatattgatt
    55921 tgcgtgaaag gatgcagttt gccgcaacac tcgccggaat agtaatcgct catacgggaa
    55981 caactgcagt tcatgcagct ggttatccac tctcttcatt caagaatgta aaacacggta
    56041 tggcaaatgc tctaattttg gtcgagatct tcaagaagat tgcggaggcc gacaaagata
    56101 gaataggcgg tgcaatcgat ccttttgaga gtctcgatga actccagatt tttatacaga
    56161 ggtttatctc tggaagaatc tccttgagaa tctcagatga agaaataaac gaatggtcag
    56221 attacacctc aaaagcatcg cacctgaaga agactccagg tgatttcgac aaagagttct
    56281 tcatcgatct atacaggagg atcaataaca tttgagtgca agagttgtct tgaagcggtc
    56341 gataaaaagg aggatcgcat ttggccatcc atggatctat gacaatgaaa tcgaaaaagc
    56401 cgactctgtt gaaggtggtt gcatagtaga tgtattgacc aattccggac agtttctggg
    56461 aagaggatac tataatccgg actcttccat cagagtgaga ctcctaacaa gaagaaactg
    56521 cagtatcgat actgatttct tcgcccaaaa gctaaaaaga tcattgggga tgaaatcttc
    56581 tttgcttaga tatggcaatg ccttgagagt tacgttcggc gaagccgatg gactaccggg
    56641 attgatagtg gataaatttt ctgactggct tgtcgttcag tttaacactc taggaattgg
    56701 agttctcaaa aacgagatca ttgaagcttt gatagccaca ctgaagccaa aggggatatt
    56761 tgaaaagagc gagggaatct cccttacaaa agaagggtta gaatcaaaag aaggatggct
    56821 ttttggtaac gggcctgagc tactgccttt ttcattgaat gggattcact tctttgcaga
    56881 cacaaaaggg caaaagaccg gtttcttcct agatcaaagg gacaacgcag agaatctgtc
    56941 aaaatacgcg aacggaagaa atgtgcttga tgttttctca tataccggca acttctcatt
    57001 tcattgtttg agccaagggg ccaatagggc gacgttggtt gactcttcgg aacgcgcact
    57061 ttcagttgca agagagacgg ctgaaataaa tggattcatc agtagatgcg agtttgtcag
    57121 ggcaaatgca ttcgactatt tgagagaaga atcactcgcc ggtcacagtc ttgtgatagt
    57181 tgaccctcca gcaatggcaa aatcaccgtc ttcgaaaaaa agtgctttga gagggtacaa
    57241 ggaactcaac cttagagtga taaaaaaggc tgtaaacgga tctcttctgg catcttcctc
    57301 atgtacacaa atcatttctg aagatgactg gatgagaact atcaacgatg cctttcacga
    57361 cagcaaaaag gtcggaatag tctcatttag agggggccaa ccgcttgacc atccggaggt
    57421 tagctctata ttcgagacaa gatatcttaa gttctgctta tttcgtgtca ttgaactgtg
    57481 cgatttctaa atgcagcgcc agatactgtt gaagtaccaa tgtaatgcta taattcagaa
    57541 aagtctaacg gctggaggta gatagatatg gccaaagtat acgttgtgac ttccggaaaa
    57601 gggggggtcg gcaagactac aattacagcc aacataggtt gtgctcttgc aaataaaggg
    57661 gctaaggtct gtcttatcga tgcagacatt ggtctcaaaa acctagatat tacacttggc
    57721 ctcgagaata gagttgtgca tacgattcta gatgtcgcca atggcaaggt aacggcttca
    57781 gaggcgcttg ttaggcacaa gcagattaaa gggctctatc tacttgcggc ctcgcagata
    57841 gcaacaaagg aaatgctgtc acccgaggat atgaaaaaga tggttgcaga actgtatccc
    57901 aagtttgact atatcatcgt cgactcacct gcaggaatag agagagggtt tagaaatgca
    57961 attgctgcag ctgaaaaggc gctgatagtg actactccag aactgccggc tatcacagat
    58021 gccgacagag ttattgggct tctcgaaaac tcgggaatgc aggagacaaa tataaggctc
    58081 ctgataaacc gcttcaaaat ccagatggtc aaacgtggtg atatgctcac cagagaggat
    58141 atccagggta atctcgctat tgatctaata ggaatcatac cggacagtga cgaagttatc
    58201 attgcgacaa acaagggtgt accggttatt ctaaacggaa acggtgaagg aatcggaaag
    58261 gtcttcgaga acattgcttt gaggatgcag ggagaaccaa ttcctgttga gcatgacatt
    58321 cttgagcacg gatcaaaagg attccttgaa ttcctgaagc gcatcttcag aagaaactaa
    58381 gagggagttg aagcgtatgt tctttggact tttcaggaag aagaaaaaaa ctgagggaag
    58441 tagaaaggaa gcaaaggata gacttgattc aattaccgga ggaaggagat actctgttcc
    58501 tgtaagggag gttattccgc aggatattct ccagaatagc ggaaaagata tggtacacca
    58561 gataaaaacc tatgtcgccg acaagtacaa agtcaaagaa gaaaacgtta aggttcagct
    58621 tgaggaacac aacggatatg tggtaataat cacaaacgtt gtctttcact gatgcaccat
    58681 gcgatacagc gtaaaaacgg aacgcaaaag tcagttcata gacattacga agcttgttat
    58741 ccagtctgta aaggaatcca aggcttctta cgggcttgtg aatgtattcg taccgcatac
    58801 gactgctgca gtaacgatca atgaaaactc cgatccagat gtcgtgcgtg acattctctt
    58861 ttggatggaa gagagcatac ccgagaacag agaattcaag cacatggaag ggaattccga
    58921 tgcgcatata aaggcgtctc tggtgggttc ttcagttact gttcccttca gagataattc
    58981 attgatgcta ggaatctggc agtgcgtcta tttctgtgaa tttgacggac ctagaaaccg
    59041 ggaggtaata gttacggtag ttgggtaatg cttcttttgc cattgttaat agtaaatcca
    59101 ggcattcatt aggggtacgg tgggaggcac tatgtgtaag ggaagatact tcttttatac
    59161 catctttgtt cttggatctt cgctgttcgc tattaaaggt tttacgtggg acatgggcct
    59221 accaaagttc tatttcgcat cggtcgccat atctgttttc atcgtctttg tttctctaag
    59281 ggcggttagg ggatcctaca agctgagtat ttcatttcct caggttcttg ctttgttgtt
    59341 tggcatttac gcatgtttga cgactttcca gctgctggat actctaccca atgtctttct
    59401 gacatctctc ggttttgcga tgaatttgct gttgttcctc ctcttctcgt tactgatatc
    59461 tggagaaaaa cccgatagac tgatgtttat ccttcaagtt ttcgttcttg cggggcttgt
    59521 catagcagtc gatgcacttt tctcctttta tactggccac ggtttgctgt ggggaacgga
    59581 gaacaaccca tttacaagag gaaacctatc ttcagtcatc ggcaatgtga atttcacaac
    59641 ggacctcatg gcaatgttgc tattccccac tgcctttctt actatctcaa gtagggttct
    59701 gtggaagcgt gaaaagctca gacacatttt ttacctgggt gttttctcca ttttcctgat
    59761 agtgatcttg attggtcaaa ctagagccgt atattactcg gtaataggat catttatctt
    59821 tctagcggtt ttcttaataa tctcacttct gaagtttagg ttgaggccct ttgccgcagc
    59881 cttcaataaa atgttcctaa tcttgctttt attcgcggcc ataattataa tgattgtata
    59941 ttctggagac aattttctga cgggtgggcg gttcagtttc tctgagagac taacctatac
    60001 aactgaagac aacatctcgg tggacgtaag agttcttcag tggaaggcag caattaaaca
    60061 gtggaaaagc tcgactatct tcggtactgg ttttggttct tacaagtatc tttctacaga
    60121 gaacatggga agggttcttc aggaagagcc tgattacatg tatgttgctg gcctgaacag
    60181 tatccgaact cataatgaat accttcagca actgggtgaa acgggaatag taggaatggc
    60241 tattgttgcc tggtttattc tctcaatgtt tctgtacaca atcagggttg taaggcgaac
    60301 ttcctcaatc gataagattg tccagtacat ttttctcacg gccggtctcc ttgtaatttt
    60361 cgcgcattca acactatctt tccctggtca tctgatgccc aatgctttat ttgcagtctt
    60421 tctctttggt ttcctcatga gccccgattt taatgaagaa agaagaatta ctttggatgt
    60481 tggaaaactt gccccgctca ttttggtgat ctttgctttg accaccactg tattgatgag
    60541 ccgcactttc ttttcagaag gttttttcac caagggctac atagattata gaaggattga
    60601 ggccataaat cctcaagtgc cacagctgat aagctccata gaaagcatca aaaaagagat
    60661 caaggctctg gagaaccttg aagataagta tgcgaatctt caagaaggac agtatatctc
    60721 tgaaaggcta aaaactcttc aggagatgta tccaagcgca ccgaccgaac tgctcgaatc
    60781 aatagcatcc gaggaccgtg aagaagcata tgaatctgcc ctgtcggggc tgaattctaa
    60841 actgagatct gcttcttcgg ctcttcttaa tgcgaggagg gattcaacag aaagttttta
    60901 ctcggccttg agaaatctat caactgcaag agagatttcc ggtagtcagt atcttgcaga
    60961 agcatacctg gggtacgtgt acttgacacc tcagagaaag gaagactttc gacttaagct
    61021 caacatgtca ggcaacaata ttggcaatgc cttcggagag atattttcga gaaaagacat
    61081 cttctcttcc tggcttacct acgacgcatt accttgcgga atgctgcctg gactggaaat
    61141 cgatcacgct tatctcaaag aactgcaaag tctgctaaag ccggatcttg ctgcgacaga
    61201 tgtttccaac cttttgaaag aagtcagcac agaactattg atcgattatc aagtaactct
    61261 tgatgctatt gatgcgcttt tgcgatctat aaagacatca cccgacctgc aggttgtaag
    61321 aaatgcagca agtctgctct ttcgagtaat tgtctcatca gagagagttt cccttgagct
    61381 cgagaagctt gaaccttacg taactgatac cgttggtttg aataatcttg tgcataagat
    61441 acaagagatc cctgtttcag agaaagacaa tctgaatctg ctttatgacg tagcgatagc
    61501 ctataatcct ggaggatggt taaaagggaa tgacagcata tatggagagt acgcaagaaa
    61561 tcttcttcta gcttacggac taaatgctct tgacaagata ctggagctgg cagaaaagga
    61621 agttttcgcc tgggacgtga tgagaactac tgaccaggtt gttcctcttg gagcgatcgg
    61681 tgagctaact ccactaaaag agtacgttcc cagagagtgg ttccataacc tgtacgatgg
    61741 ggtgtatgaa tggtgtctgg ataagtcctc ggagatttca aggaatattg aagaagaaag
    61801 gtttgccgga gaaaacctga agaaggcaga aacagttttg aatgaaatgg agagattcaa
    61861 aggactctac tcaacctggt aaggaccaaa agtcacacgg gtgttacaca ggtaatattc
    61921 gaggcattgc ggactttatt aatcggactt atgatagtga tgatcgcaat ggaggtatgt
    61981 ggatgctaga agttaaagga ataaagttgg ttcgagatga gcgagagatt ctaaacaccc
    62041 ttactgccac ctttgaatcg gggcgtgtct acgcgatact cggcaataac ggcgttggaa
    62101 aatcaacatt atcatatgtt ctaatgggtc ttgaatctca cagagactac gaaggaaatg
    62161 ttgtctttga tggagaagag atagaccttc tttctgtatc tgagagagca aggaagggta
    62221 ttactcttgg ctggcaggaa ccggttagat ttcaaggatt aacagtaaag gagtatctga
    62281 ctttgggtgg aaaactcaag ctttcaaagg aagaactcaa tgagaccctg tcagttgtag
    62341 gactaaataa ggaatatctg gacagattcg tcgatgaatc ccttagcggc ggagagagaa
    62401 agagagtgga gcttgcctca ataattcttg taaatccaaa ggtaacaatt cttgatgaac
    62461 ctgattcggg tatagacata atgtcaatgg aaatgataga aagagtgctg gaaaggctac
    62521 gtgacaaagg ttcaatagta ataatcatta ctcacaggga agaaatcgca aggatggctg
    62581 acgaagccta ccttctttgt ggagggagaa ttctggcttc cggcgagcct gaagagatag
    62641 ttaatttcta cagaaaactt tgcgataact gtcaacacat aaatgaacct ataacagaag
    62701 gcgggcgggt agatgattaa ctcaaacttc gaaagagaat tcaccgccat tgccaaagag
    62761 tacgagagat ctggtggcaa tgtttccgat ttcttgagaa aggacattgt atcgataatt
    62821 gtcagcggta ataaggtcat tggcagaaac acggttgacg gagtccacgt taaagcaaag
    62881 gaactaaaca atggagtgga aatctggctt gaaatcgatg atggaacggt tattgataac
    62941 cccattcatc tctgtactgg ttatctcaaa ccggaaggta cccaagagat cctgattcac
    63001 aaccatcttg gagaccacac aaaggcaaaa ttcatatcgc actgtgtttt ccccagcggt
    63061 gtaaacttca ctcattccat ggtcgcagat accgatgtgg ggaagtactc cgaaatgctt
    63121 tatgaagaca ctcacatgca cagcaaagat ggtggagtga ccgtgaaggc aacctacaat
    63181 acagtagtta gagaaggcgg gagtttccag aatttctttt atctaactaa gaccagggta
    63241 ggcaagctct tcgtaaagat gaatgttgat cttgaaaaaa acgcgtcggc tcacatagaa
    63301 tcaaaggttt acgagagaga agacgattat ctcgaaatag acgagcagct gtatctcaat
    63361 ggcgaaggct cttcgggaat agcaaagacc acggtttttg caacagacag gagtaaagcg
    63421 aagatcatca ataaagctta tggaaatgca gcttattcaa agggacacat agagtgcaat
    63481 gagataatta aaggcgactc tgttgaggtt ggaacagttc ccgaattata cgtgagaaat
    63541 gaaaaggccg agcttaccca cgaagcctcg atagggagag taaatgtcaa acagatggag
    63601 acccttatgt ccaagggact aactgaagaa gaggcaaccg atatgatagt tagaggaatg
    63661 ctcagataaa atcggaggga gtctttgtga actacagtga actgtatata gtgaaagagc
    63721 ttgtcgatct cgtcaatatt cccagtccat cgggatttac agattcaatc atcgtttatc
    63781 tcgactccga gttaaaaagg cttggattca gtcctcgaag aaccagaaag ggagctcttg
    63841 tagttgaaat aggtggaagg ggaaacccaa tagtacttgc cgctcatgtc gatacgcttg
    63901 gagcgatggt gaagtctttg aagcctaacg gccgcctgga aataacgaac gtcggtggtt
    63961 tcacaatgaa cagcatagaa aatgaaaact gcatcataca ctcaaagacg ggaaagactt
    64021 tcagcgggac tattcaatca atttcacctt cagttcacgt tttcgaaaat gccagatctc
    64081 ttgaaagaaa aatcgcaaac atggagatac gtgtcgacga agaactcaag aacgccgaag
    64141 atctacagaa gctgggaatc gaggcaggtg attttgtctc tttcgatcct agagcaaatg
    64201 tgacaaagaa tggcttcgtg aaatcaagac acctcgacga taaagccagt gttgccgttc
    64261 tgcttgattt tgcaagaaga gtttctgaag gcaaggtaac ctgttccagg aagacttact
    64321 tattcttctc aaattacgaa gaagtgggtc acggcgcatc ggccgctatg ccggatgaca
    64381 gcgaagaaat catctcagtc gatatgggtg cggttggcga tactcttgga actgacgagt
    64441 atgtcgtttc aatatgcgct aaagattcgg gaggccctta tgatagcaca gttgtcagaa
    64501 gccttattga aacggcaaaa agagttggtg ccgactattc tgttgatatt tacccattct
    64561 acggttcaga cgtagaagct tctttgagag cgggatacga tgttaggttc ggtcttattg
    64621 gaccaggagt agaagcgtct cacggatatg aaaggacaca ttacaaagca cttgaaaaca
    64681 ctttgaagct tattgaggga tatataggaa actgaggcgc tatgcgcctc agttcatctt
    64741 ctaaaacagg agcttcccct ttttctgacc cttttcctgg ccaagctgat tcacaaaacc
    64801 cgcaggagcg acatcgatta ctctgttctt ttcggcttcc atagcttctt gtagtttttt
    64861 tctgtatgca tccatttttt ctctcagttc agctacatca ccctggagcc attcaaagat
    64921 gctgtctgtc aatcttgaag aaatcttctc aatctcatcg ctttcttcca tcataccggc
    64981 gttctttgtg atttcaagct cctcactaac tgccttttca acatcgtctc tagtaagttc
    65041 cggaacaacc gacttaacca aacgcatggc gatatttgtt ctcagtctta aatcgtctat
    65101 agttgcctcc agattatcta ctctcgacat gattgctccc aaaaactgat caagtgatac
    65161 ttgcatttgt tttcaactcc cttcaatgat ttcgcttaga ttgtactacg cattgagttt
    65221 tttatagtta aggctttgtt tgggatcgta atctacgtat tcttgtctaa tctatgcctc
    65281 attctaaaaa gccatatcga caatactgat accattagga agccgacgag atcgccttcc
    65341 gatattttga agatccaaaa ggatggaagg aacttaccgc tgaagaatgg agcggagaga
    65401 aaatcggaaa atagccatcc cgagataatt gaagcgacgg taatgaaaat ggccaggaca
    65461 ttctctctta tccttctgag ttctttgaag ataagaaagg agaccgatgc aagagaagta
    65521 actaaacaaa tggaagaaat gatcgcaaga atcgagtaag cacctggatt acgcaatact
    65581 attatcgtaa ctattagaac cattgttaat ggtctcttca gtactcccct gaccatttcc
    65641 agtaaactgt ccttcgaaac gattttattc tccagctccg gtctgtaaac aagcaatcca
    65701 agtacaatcg caattagcaa aacagcactt gagagagagg cgctccaaaa aaacatcgac
    65761 acagccagca ttcctattag agactcctga acaagaagaa gtcgcatctt caagcttccc
    65821 cttcgaaacc ttcaatagat agtttggttt ctccgtcgcc tgttatatca aggaagagtt
    65881 cctctagact tgattcagct cttccaaaac tctctttcaa atctgaaaga gtaccttcag
    65941 cgatcagcct tccgctatcg attatcccga ttcgatcaca catcttttcg gccacttcaa
    66001 gaacgtgagt agtcataaac acagttgttc cattatcggc catatttctg agcaagattc
    66061 tcagctttcc cgcgctcgag gcgtctagac caactgtagg ttcgtcaaga aagaccactg
    66121 atggttctct catcagagca attgcaacca tcagcttctg tttcattcca tgcgaataat
    66181 cacctatgta tttcccaaga taatcgatct tgaaggcttc agctagatta ttgagctttt
    66241 taagcgtttc cttcactggg agacgataga cgtcaataat gaatttgacg aattccagcc
    66301 cctttagatt ctcgtacatc tttggttcat cagggacaac tgaaatcaac ctcttgatct
    66361 ccatcaggtc cttttgcatg tcaagaccgt cgataaatat ctctcccaga ctgggagaga
    66421 ttattcctgt gagcatcttt atggtagttg tctttcccgc tccatttggc ccaagaaagc
    66481 catatatttc accggaagca atgttgagat tcaatctatc tacagctacg agagttccaa
    66541 actttctcgt aaggtttcgg gcttcaatca tttttcagcc tcaaaggctt tatcatagaa
    66601 agttgcggtc tttggatgga acattagttc tacagtaccg actggaccat ttctctgttt
    66661 cccgatgatg acttttgtta attgtggtga atcactgacc tcactcttgt ctttgtagta
    66721 gtcctctctg taaagaaaca taacggtatc ggcatcctgt tctatggctc ccgattcgcg
    66781 aagatcactt agacgtggca ttttatcttc tctttgctcc actgcccttg aaagctgcga
    66841 aagagcaaca acagtgatgt tcaattccct tgcgaggagc ttcatagatc tcgaaatctc
    66901 cgagatttcc tgctgcctgt tgtccgatct gcttttagta tgcattagtt gaaggtaatc
    66961 gacaaataga acctgaatat tgtgttccat ctttatccgt cttgccttgg ctctcaaggt
    67021 tctaggatca agagatggct catcgtcgat gattattggc gcggtctgga gagtactggc
    67081 tccatttgtt agccttttcc actcctcgct tccgatatct ccggcgcgaa gccgagacag
    67141 ctcgaaattc gtcacattac acaacagccg ctgtgcaagc tgttctttgg acatttcaag
    67201 gcaaaaaaca gctactggta gctgaaactt cagcgccata ttgcttgcca ggctcaaagc
    67261 aaaggcagtc ttacccatag aaggtcgaga tgcaacaatg acgagatcgg atggtcgaaa
    67321 tcctgtagtg acttggtcga gtcttctgta cccagatgct attccggtaa ttcttcctgt
    67381 aagggcgttt tctttcaacc tttccagact gtgaaacagt tcatgcatta tctttccaat
    67441 gtgctgatag gttttggata tctgggcttc ggtgatttga aaaatagctt tctcagcgtt
    67501 gtcgaggatc tcgtccgtgt cttctccttc gtatgcgttt tcgacaatcg aacttgccgc
    67561 tgctatgagc gatcgaagta atgacttctc ctttactgtc ttaccataat aaaagaaatt
    67621 cgcagaggtt ggaacgacat cggctagctt tgccagctcg acttcacccc caacttcctc
    67681 aagaactccg ccggtcctca gtctttccgt caccgaaact atgtctacag ggcttccttc
    67741 atcgaaaagc ttctccatcg tggaaaaaat aagctgattc tttcgtgagt aaaaatccct
    67801 gcctgtgagg agttccatga catctggaac aacatctgga tctatcaaga tacttccaat
    67861 taccgcctct tccgattcaa tgctgtttgg gggtactctt gtcttcaaat cgctcaccaa
    67921 cttcttctag ataatcaata gctattctca atcagctcct ttattgaggc gaactcaccg
    67981 tcgaaaacaa attggcctct ctctttgccg gatccacctt tgacgtcgaa ctcctttttt
    68041 atcctggtga gaacctctcg gcaatcgtat cctttacaaa aaagaagaat tctatcatca
    68101 gactttccaa tgataagata ctcctcaagt gtgacaaatc ttggaatcga agcgagtata
    68161 ctctcttcat cctcaagaaa gataactcta gactttgatc cgtttatccc ttccgcaaca
    68221 taacccgcta gtctttcaga aaagcttctg attgtcgaat taccttgttt gatttcatta
    68281 attagagacg caattctatc cggcaaatcc aaataactgc acgttagtaa gagggaggaa
    68341 tttgtgatca atttatgttt agtgaaataa tcattcagag cccttttccc tgcaacaaaa
    68401 tagactctgg tgagcgaccc tttcactttc tcgtgtttca aaatcttcaa taaaccaatc
    68461 tgaccagtac gatccacatg aagacccgag caggcactta ggtctatccc gtctatttcg
    68521 acaagcctta tctctctttc gctttcagtg atctctctct tgatctcttt tctaagacga
    68581 tatttttgga tttcagagct ctcgacaaca aatttcctta ccgcacgatc ctcaccgata
    68641 aaattgttgc aaagcctctc tacataatcg atcatatcat ccttaagaat tccaagtgag
    68701 agatcgatag tcgtgtactc ctcgcccatc tgaaatccta cagtttcggc atccatttcg
    68761 ct
//
LOCUS       DAOQYI010000007        68121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_428_length_68121_cov_71.365910, whole genome shotgun sequence.
ACCESSION   DAOQYI010000007 DAOQYI010000000
VERSION     DAOQYI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 68121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68121
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_428_length_68121_cov_71.365910"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PKL93_03780"
     CDS             <1..723
                     /locus_tag="PKL93_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNQ70415.1"
                     /translation="RVFGLWVGAQMFVYNIVLFLLAFVTLGVGFGMKSIYSAVLMSLS
                     VDLLQKLSFPVFDASSTQDGFLLVAIYGGVIAGAGMGLVLWRGASTGGTDIVAMILSK
                     YVGFSTGTGLLISDSLITLLAVVVFGPIAAMYGIITIFTTSKTIDGIIEGIGNTRTAF
                     VISKESEAIKEKVINEMERGTTIIKATGGFSGEDRPVLMVSIRRREIGQLRHIVKTTD
                     RRAFVVVVNNSEVFGEGFKDLS"
     gene            732..1391
                     /gene="tsaB"
                     /locus_tag="PKL93_03785"
     CDS             732..1391
                     /gene="tsaB"
                     /locus_tag="PKL93_03785"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNQ70416.1"
                     /translation="MKYICFDTSSRTLLLSAVSNAAEMDIRCRDIDRYGSMLAVIMRQ
                     AIENLKIDVSELEFVGVGIGPGSLTGLRVGISTAKGLAFPHGIPAVPFNSLDVLAKTV
                     DSDRYLILRKAREDHYYWREYKGGLPVGNTGFSNTGMIKSKIELSTMELFFDGEIDPE
                     FEDFRCSLVKNYDPSSMREIVLDTYASGNQVSCSDLAPIYLQKSIAEINWEKRSNEKE
                     S"
     gene            complement(1388..2572)
                     /locus_tag="PKL93_03790"
     CDS             complement(1388..2572)
                     /locus_tag="PKL93_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNQ70417.1"
                     /translation="MELNFPRYDGSSIVNFSAAILEHFDADIPQGVKAYSLKDNGLSL
                     EGIDKVVVFIIDAVGYYNLIKVLEKKSFKYFDQKDIRILTSVFPSTTATALTSIFTAT
                     PPGEHGVLGYLLNVPEYGGLVNMIELAPYTQDRDNLTRLGFDPLKYNPNPTIFELLKE
                     VGVRGYHLTSKSFVNTGLTRMHSRGGIARGVHGLGDMFEELRTALFSEEAASVTLVYW
                     GLIDTYGHRYGPNSLSYTSETATVISAIEEFFEDIAERNTAFFITADHGQIETPWEQE
                     IWWSKFDDLFEEMYSMPGGEQRMSYIYSYDGEKTKRKMERQFGDALEIIEPSGLDEIK
                     LFGKPLNSVFRKRRGELITVSKGNSSLCFKYTGQEHSLKGRHGGLTKEEMYVPLILLR
                     KP"
     gene            complement(2853..>5018)
                     /locus_tag="PKL93_03795"
     CDS             complement(2853..>5018)
                     /locus_tag="PKL93_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ70418.1"
                     /translation="YRKLYEDVFLYRDETLDAIKELEFENKDEVVRSLESIENYISLS
                     SIDYSEWRRVEWLRVINRYLRDVESTMPQAEQDALNGVRTKLYDSFKAAGDAWEEVEA
                     AAEKVSQDLSLLAEEAFGPEYHEYVSAIEELGVVEADKEAVEKALATSESALAELEDS
                     FEVALPTILSEKSKLSALIPPLQIVISKGVEEYTAVSEARLNSSLDEVDSARKTIGLL
                     QQQLAEMQNVQELSSSLSELLGEMAWFSDNKEALANASGNSEVSKGIDVINIAISNLS
                     RILPVLETSISEYVEVSENTTSLRKELKSLEERSEILGSRIAELKEEADRAEAEYLMA
                     LESIRIKSTLALVEEEIASLEDGRAYVSKTTDILDTYFGIRASNRYRTLGWYDDFDRA
                     NREARDGTALLNQLISTMRDMKEELSSKVNHYIDLRFLGTTVTLDDFGTMQETYNSLF
                     QQVNARYQRASRLISDLIEKPASYSASYDRELREIDKALFRANQIWVQKESTYFYFVR
                     WLLNSVIVALSVSLISVAVAALAAYPFSRMRFRGRKQGLLGLLLIQMFPTIMFMVALY
                     ALLQFIGGLIPFLGLNTLGGLIFAYSGGIAFNIWLIKGYYDTISNSLEEAAMIDGATK
                     FQAFTKVILPLARPILAVVAILTFMSIFNEYLIARILLQDMNKWTYAVGLWQFSGRFE
                     TSWGPFTAAALIGAVPMVIFFLVLQDYIVGGLTQGGVKE"
     assembly_gap    5019..5118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5119..5779)
                     /locus_tag="PKL93_03800"
     CDS             complement(<5119..5779)
                     /locus_tag="PKL93_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease"
                     /protein_id="HNQ70419.1"
                     /translation="MAVERRKYWIRHLILIVVVVVVLFPMVWLVSTSVRRDQAAFSSN
                     LFSSRITFQHYRNLLFPERSVGRLILDLQSATYATGEYRGRSQEDLKKTVERYLVKFE
                     DLMDESEEMVASIENGTSAIEKELKEKESLIIREMNEVRLRDKALFEERLQEIGELSE
                     VKMAYAVGELLETTAPSLEGVYPFYIDLLGERADPIVKSLETYRKLYEDVFLYRDETL
                     DA"
     gene            complement(5779..7521)
                     /locus_tag="PKL93_03805"
     CDS             complement(5779..7521)
                     /locus_tag="PKL93_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ70420.1"
                     /translation="MSRKRFVIFLFSLAILAIFNAFSLWSVYLLFADGNFGLMVTMIV
                     LTLLIDIIALNPKGYPYRYMIPAMILLFILTLYPMYYTFRTAFTNYGTGHLFTRQQSI
                     QKLLSDYFYIPESPEEFEFSIFIELDDYKPTDRFITLLKSKETGALFAALRPQPVTKD
                     AEGNITLATAKMVQVSGDSFSTGGVSYTLSISPDDKILAIRATSGERFIYFYSPQDDS
                     TRPNAPFYFSEIRGIWLRNAEFTDSEGNQVRLFPNSLYTTFATTERKYALRTETTFDA
                     GRAVQETVVYNRQTNRTLVEEGGFFYDIDENGNEFAVEGYISDVGFWNFVRMFRDPKI
                     SGPFFQVFGWTFTWAGLSVLFSFTIGLALAITLNDKRLKGKKIYRTLLIIPWAVPAFI
                     SALVWRNGFFNETYGVLNRFIIQGLFGLEPIKWLNDAFWAKVSVLLVNTWLGFPYMMT
                     VSLGALQSIPEEFYEASSIDGATKFQRFRKITFPLLMVTVAPLLVGSFAFNFNNFTGI
                     YLVTQGGPAIVGASTPAGATDILISYTFKLAFEGRGQDFGFASAISILIFIIVAGFSW
                     INFKLSGSFEEVSR"
     gene            complement(7611..8801)
                     /locus_tag="PKL93_03810"
     CDS             complement(7611..8801)
                     /locus_tag="PKL93_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ70421.1"
                     /translation="MKKLAAVLLMLFVLLSFAGAQLVIWSSENQIPALEKLAADFESD
                     YGIQVEIQQVNFGDIKSKFLTAAPAGEGPDIIVGAHDWVGELALNGLIEPIPFLPDSD
                     QYYEVALDAFSYGGKLYGVPYTIEAIAIIYNKDLVFEEPKTISELEELALEASDDEVV
                     GLIYTAGDFYHSAPFILGRGGYIFKETADGLDVTDIGLNNEGAIAGGKLIKSWFDKGL
                     IPAGPNYNLMDSLFKDGLAAFIVNGIWATPGYRDAGIDYAILPFSEITFDDGTTPRPF
                     VGVQGFMINAKSKNKLEATEFVVNYIGSFEGQYGMFLGERRGTARADVFSFIEANAGP
                     ELYDVLQFSKSASVGTPMPNVPEMASVWGAMADALGLIINGQDTVENAFNSAVEKIKT
                     SIGN"
     gene            complement(9003..10397)
                     /locus_tag="PKL93_03815"
     CDS             complement(9003..10397)
                     /locus_tag="PKL93_03815"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HNQ70422.1"
                     /translation="MRKVCFVDTTMRDAHQSLLATRVTTGELAEIAPLVDRVGYHAVE
                     VWGGATFDSCVRYLNEDPWERLDILKESFKETKLQMLLRGQNLLGYRNYADDVVELFV
                     RTMAKHGIDIVRIFDALNDFRNLEKSAKAVKESGMHLQIAMSYTTSPVHNVEYFVEIS
                     DKAEELGADSLCIKDMAGLLKPGTAWTLVSEIKKRHSIPLEVHSHFTTGIAGMTYLKS
                     AEAGADILDTATSSMGFGTSQPGVESVWTALHDLGLADKPDYVLLKEINDYFVRVRKN
                     HSETDAGFTINTSVLENQIPGGMYSNLINQLKAQNMSDRFEEVLEEVPRVRADLGYPP
                     LVTPTSQIVGVQAVLNVMNNERYKTITKEVESYIKGFYGSPPAEIESTLKTRVMGDEK
                     SISHRPGDTLEPEVEKGRNELGLLANSDEDLLIYLLLGEVGKRFLTQRYNDSLRVDFD
                     VAKNFKEGITVYPV"
     gene            10447..12333
                     /gene="mnmG"
                     /locus_tag="PKL93_03820"
     CDS             10447..12333
                     /gene="mnmG"
                     /locus_tag="PKL93_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNQ70423.1"
                     /translation="MRSNKRDYEFDIIVIGAGHAGIEAALAGARMGMKTLILNINLDT
                     VGWTPCNPAMGGPAKGIVVREIDVLGGEIAKSTDQASLNIRILNTSKGAAVRALRAQV
                     DKIRYSQTMKRSMEMQENLFIQSGIVKAIETEKGSVKGVITNFGLRYSARSVIVASGT
                     FLGGKIFIGPHEFPAGRLGEFPSTELTGSLRSIGFEVRRFKTGTPARIAGRSIDFAEM
                     ERQGTSETPLCFSHFSEPYVLEDDHPCWLTRTNKETHDIIKRDLHFSPLYGDLKLIDS
                     RGPRYCPSIEDKVIKFSSKDSHQLFVEPESAYSNEYYLNGLSTSLPYETQLEMIRTIP
                     GLGRAEIVRPAYAIEYDYINPTQLYSTLESKVVENLYFAGQVNGTSGYEEAAGQGLIA
                     GINASAKLLGEKQLVLSRSEAYIGVMIDDLVTKGVDEPYRLLTSRAEYRLLLRHDNAH
                     IRLTEYGYRYGLVPAWFKERVDSLTREIKTQLIRLQKINVRPSEGLNELLISKGSSKI
                     DLPVKFSSLLKRAGVGYRDISMFDPEPILDDDVIGQVEIEVKYSGYIDRLKAEVKRLE
                     EIEEDLIPSTMDYDAVSNLSTEAREKLKKTKPASVGQAMRIPGLTPSDIMNLSFHLKH
                     EGEK"
     gene            complement(12355..12528)
                     /locus_tag="PKL93_03825"
     CDS             complement(12355..12528)
                     /locus_tag="PKL93_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70424.1"
                     /translation="MLNSSSVGLRISTDPVQEMTVKYPRVLVIKAAFCLLKDGKSIEH
                     RDLEKTLQTLLSG"
     gene            12591..12668
                     /locus_tag="PKL93_03830"
     tRNA            12591..12668
                     /locus_tag="PKL93_03830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12626..12628,aa:Ile,seq:cat)
     gene            complement(12818..14257)
                     /locus_tag="PKL93_03835"
     CDS             complement(12818..14257)
                     /locus_tag="PKL93_03835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNQ70425.1"
                     /translation="MAYSLKGGRVYSVPDGRYVTRDLYISEGIISEVPAKDAIEIDVS
                     GKFVYPGFVDSHAHLIGTGRKELEVDLENCDSVEKLETALNVERDIVRARGWDQEVLG
                     FMPVRKILDSIISRPVIITRRCGHIASANSAFIKLFSLERLHGLDGTDIDLGILKERA
                     LDEVERNVLLTSGEVEAAIEAGTKAFLKYGVTSVHSDDYHGIEYELLMQRLAGIVDLR
                     VYEKFCVSREEDLEMILLGRQFENEFFQLNAAKLYLDGSLGARTAAMIEPYYDSPGER
                     GVLYKSAKEIRPIVKRADGEGIQVCVHVIGDRALEEALKAFEGSKSETLRHRLIHVQI
                     ASEEQIRRMVEEKLTASIQPVFVDSDKLIAPERFGPSRLENAYPFGKMRDMGVLLALS
                     TDSPVESTSVIRNLNSADRYFSRRESIEMYTSNGHLLANSCRGCSLEPGYPADLFVID
                     ADLLKTNEDRFSDMTFVGGRLVFSLSEIG"
     gene            complement(14257..15120)
                     /locus_tag="PKL93_03840"
     CDS             complement(14257..15120)
                     /locus_tag="PKL93_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNQ70426.1"
                     /translation="MKLNNVVALESTVIAHGLPRGVNFETALNMERAVRKNGGEPKTI
                     GILNGEIIVGLREEQIMRLGTAPNVLKVGTAEIPYATAMKRDAATTVSATMAIAKRDG
                     ISVFATGGIGGVHRGKDWDVSQDIIELSRTSMIVVSAGVKSILDVPKTLEFLETFGVT
                     LVGYKTDSFPLFHCSRSPYRVNLTINSPEEALLILKEKERLGIDGAVLVANPIPETDS
                     MDYDDLSRLIETALSTAEARGVSGKALTPFLLSTIAELSNGKTLDANISLLINNAALA
                     GQIAGSLGAEL"
     gene            15305..15379
                     /locus_tag="PKL93_03845"
     tRNA            15305..15379
                     /locus_tag="PKL93_03845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15338..15340,aa:Arg,seq:cct)
     gene            15562..16815
                     /locus_tag="PKL93_03850"
     CDS             15562..16815
                     /locus_tag="PKL93_03850"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNQ70427.1"
                     /translation="MAETSLFQNALKQFNKAADVMDLSQSMRQVLSFPKRELTVNFPV
                     RMDNGEIRVFTGHRVQHNVARGPAKGGIRYHQNVTLDEVKALAFWMTWKCAVVGIPYG
                     GGKGGVCVNPQELSINEIERLSRRYFSEIQIIIGEGEDIPAPDVNTNAQIMAWYMDTY
                     SMNIGHSVLGIVTGKPLEIGGSKGRTEATGRGVRVVAEEALRYKDMDPKKAKVAVQGF
                     GNVGSYAAKLIAEEMGSRVVGLSDVSGGLYNPDGFDIDDLIVYRDQNNGVIEGYPKGQ
                     KISNEDLLSLDVDVLVPAALENAITEKNARNVRAKIVVEGANGPMTPEAEDMILANNI
                     FVVPDFLANAGGVTVSYFEWVQGLQHYFWDVEDVRRALHKIMKEAFGSVISTMNKYSI
                     DMRTAAYVDAIEKVALATKLRGIYP"
     gene            complement(16888..17538)
                     /locus_tag="PKL93_03855"
     CDS             complement(16888..17538)
                     /locus_tag="PKL93_03855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNQ70428.1"
                     /translation="MAVADEGHFGLTYAIGDIHGCLSPLKRLISALDLKDDDSLVFIG
                     DYVDRGPNPRGVIDFLIALSANYECHFIRGNHEQMFLDYLNGVQGSTIWAYNGMETTI
                     RSYGTIRSIPEYHIEFLNNTVLYYEEKSFIFVHAGVRPGIPLKKQDPKDLLWIREPFL
                     ESSYPIKGRTVVYGHTPLISGPLIQEGKIGIDTGCVYGGFLTAYRVDDNFYLSERL"
     gene            complement(17505..18122)
                     /locus_tag="PKL93_03860"
     CDS             complement(17505..18122)
                     /locus_tag="PKL93_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70429.1"
                     /translation="MPLHLLVAYYVVDHAFVNNKKLAKMDDKKFWMHFVWVVLVFLAF
                     TFDVFLSNPLGILILILSIGLTVTLDMGRKRLSNELIEIIAFFLLLFLTLLGRSFLIE
                     SFVTVEFSWYLMGMLLTTVGVTYFLRGRIIKEEATDSIGIAERMSIFIFILANQWTWV
                     IISVAAGLAFRAVFSKEKKTEWIISPVAGVLISYLWQLLMRGILG"
     gene            complement(18126..20534)
                     /locus_tag="PKL93_03865"
     CDS             complement(18126..20534)
                     /locus_tag="PKL93_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNQ70430.1"
                     /translation="MRLSEFVIRHRVAVIVFFVILGAFGAYLSTKLNIDSDLLKILPK
                     DNPVVKQYNRFISGDTSGDVTYIVLQTDDKTEKGIENLISAGRQIYETANEMKNIESF
                     VRFDVMSDLGPLGLLMVDPAELSSMRSRDQDLSRTVQSLVNYDFSAVRSIGQMLVEFE
                     ELRGLLGNSPQSDEFWNFVRLPETIPTNDPMVLVMGIRLDGPSSDVSYVNRVIPLLRR
                     WVGEICSSYGLSFGMAGDHFGTYDSNKQVNDDFMLTTIISLVGISLLFFFAYSSLKIT
                     LYVFFSLAISMFITLGISYVVFGSLNIITTFVTAITLGLGIDYGIHMITRLSDGASKQ
                     KNAVALLRDAYGALTRPLLASMITTALVFIILAAIDSPAIRELGILSSIGIVVFFVVM
                     IVFLPALSIKTLINSTKVANIHLLDRFFQKISRVIIRFGVISGGVVFALILILSYLGL
                     NNIRSFSYTPPGLMSPDSEQVAIPNLIERTFGGSIINTVPFILPDLDSLRKAHEEIDQ
                     NPNFKSSFSILSIVEGGKGDYMSQMEQVVREIDALRDSPLVEAVFKKANYYDFIVELL
                     DRAGTVESSDDLISLVTEVIPESLRDQLLYLGGDGKTYFLLNSEPSSYIYRNNVIKII
                     YDSLSPELKETFGGYPKVFYYLMDLVRRVSLSICLVAFLAIFVVVAAERKSLIDGFKV
                     LLLMIGILMSMFGLMELLGVDTTFVTVISAPLIIGIGVDGLVYVMHSSKRKGNSELSK
                     TLKSVTMSSATTMLTFFSFVFAKGKLLSAFGISLGVGVLAALIVATFLVPVLPWNSRE
                     KIGG"
     gene            complement(20633..21307)
                     /locus_tag="PKL93_03870"
     CDS             complement(20633..21307)
                     /locus_tag="PKL93_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70431.1"
                     /translation="MKIASVSILISLIALICGLLVIVYMNFETIQNVGSRRATPDFFL
                     RNSFIPLLERFKNHLEETKDFSSVLQQLEREGVGPWIMSAEKGIRTFTCPYKEVFSRL
                     QTLFYIETGKNGIREHETVEFFGSTVTRMESIDYGAFRLTYLPGYTLVMESDYNSFLK
                     EGFRFYRVHFMHSISPGSFVSDVFAGSGPVVVLREGGVINQSFIETVERYSIPYLPVR
                     YFLIKD"
     gene            complement(21304..22542)
                     /locus_tag="PKL93_03875"
     CDS             complement(21304..22542)
                     /locus_tag="PKL93_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ70432.1"
                     /translation="MEKNRVLKILLTVSVLVAVFFLGALSSMTDDEMFEKFSPVFEIL
                     TYIDRNYYDIEKVDYDAVLNETLTGTMRGLDDPFAWYFDPVQTKENELDTTSKYGGIG
                     STVQYNIEFDCLEVVAPMAGSPSERVGLRAGDLILTIDGVPVSEVGYYGAVNMLRGDP
                     DSQVVLEVYRESVENPFFVEITRAFIEIKSVKTELLTVEDTEISYIQITGFNAPTYDE
                     FQDALNLSRESGAFIIDLRNNPGGLLQSVLNISSLMLPKGKRVITIRYRDGQEEVYNS
                     WGSRYNSYFTDKPIVVLVNDGSASASEILTGALKDHGLATVIGTTTFGKAAVQTVFNL
                     SNGGEIWLPTAHYFTPNGSDIHLQGIEPDIIVEAGEQAPSEDGRELTLSTATVDVEHD
                     LQLSKAIETILEKLGVKVVN"
     gene            complement(22529..23314)
                     /locus_tag="PKL93_03880"
     CDS             complement(22529..23314)
                     /locus_tag="PKL93_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNQ70433.1"
                     /translation="MIQYGDSILIMLEHEEAKYFSEVKRGSVLKTHYGNLIMDSLVGR
                     EFGEKIRLGLRDAFLIKPSMIDGIFSLKRSTQIVYPKDVAFILLYLDIKPGDVVYEAG
                     TGTGVMTSAFSRAVCESGKVVSYEKRQDFLEKAQKNVRHLGHIERVTFVSGDVAECKE
                     SQKADAFFLDVPEPSASLESAVSILKKSGRICILCPTANQVQETVTILGTLGIIDIQV
                     WEILARSYKANPERFRPEDRMVGHTTYLVFGIKVEGRKLNGKE"
     gene            complement(23315..23590)
                     /locus_tag="PKL93_03885"
     CDS             complement(23315..23590)
                     /locus_tag="PKL93_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNQ70434.1"
                     /translation="MQGVDNETRKAVLNRLKRIEGQIRGLQKMVENDRVCGDILTQVS
                     AVNSALSKVSEMIVKGYTRKCVIEASKSGQIEELDTLIENIIKLRGV"
     gene            complement(23600..25267)
                     /locus_tag="PKL93_03890"
     CDS             complement(23600..25267)
                     /locus_tag="PKL93_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNQ70435.1"
                     /translation="MADHEIFVKELGKKIHVTKETSFFELSKMCGELHKAPIMAAKSG
                     NSLIELCRNVNGESEVEFIDLFSLDGIRIYTRGALFILFIAIRELFGSAQLNVHHSRG
                     TGLVCNIEGIESTPENLLQIEERMRELVKEDIVFEKATLRKFEAIRLFSEDGQRDKAL
                     LFKYRKKSTVNIYRCKGFINYFYGYMPYSTGAILNFSLIPYGDYFILNLPDTKQPDRL
                     PKFVDQPKISTIFLEHERWGEILGVKTIGEINEIISKGPREIREIVNITESLHEKKIG
                     AIADQIAKHKEPSLILIAGPSSSGKTTFSKRLMLHLKVLGMNPVQISIDDYFVDREKT
                     PLDENGNYDFESIHALNIDLFNEQISQLLDGKEVLLPKFDFMLGKSGFHDKPIRISEG
                     QPVIIEGIHGLNELLSSSVPRHRKFKIYVSALTQMNLDSLNRIPTTDVRLLRRIVRDN
                     RSRGHSALDTMRMWPSVRRGEDKYIFPFQEEADVMFNSAIVYELAALKGFAEPLLVQI
                     DDSVPEYIEAKRLLRFIDYLLPMMNLEDIPRTSIIKEFIGDSVFGSL"
     gene            25324..25399
                     /locus_tag="PKL93_03895"
     tRNA            25324..25399
                     /locus_tag="PKL93_03895"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25357..25359,aa:Gln,seq:ttg)
     gene            complement(25432..27633)
                     /locus_tag="PKL93_03900"
     CDS             complement(25432..27633)
                     /locus_tag="PKL93_03900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ70436.1"
                     /translation="MPNRKSRGNSNFMKSIQRPIVWAVAILFGVGIVWWSVAQYLGGS
                     SQGNEQGTGTEISFTETVGGLTKDGTPLSNSDYWITYSEYETSVRDTLTNLRSQGYNL
                     DPYFETENYPSEMGIRYDLFKSLIDQKTLLLYATENGVLPTAEQVDAETNRIVDEYVA
                     DEETKSAIIYQYGSVDAFSELVRDYVVTQMLATNVTQKAIPDMEERFENYVNENMEDL
                     KINYEKVDANHILVSDEASAVEIKSMIDSGEISFTDAATQFSIDTGTAINGGALGEFK
                     RGQMVQEFEDASFDATPGLVIGPVESQFGYHLIRVNSKTTFDTFEEFASTSAYQTELG
                     RFENEEFNKWIADYRQQNNLSYTINDPDLQMYARYDEAISDPEKSSALLEELEKDYFD
                     ESGNLLIGDSYLPVVFYTELAENEITKLRNQIFDLEDLQDLLLTLPSTATSLTIDEID
                     SRLETIPSTDTEARSVLTDAKRAKEYMSEFNVETPEEIEEALAEKRSLLDSKNLRFEN
                     SVRYLYSVMPNSARVINYMYQIDGDNPEVVYMYNETSYNTNIRPLLESPELLDSYLEY
                     YGQYFGAQARSLLLDSPIQGIESDLNQKVINSTEAATDLKMSALYLLVDIYEKMANME
                     QNEQLTQIYLLSEKHYLEKLAELYPEDETIRSLIETIKFQISELNAQNNVDATPTADA
                     TSTETSTETLIDTEIGTGELEVPLGNDEHSSIGDVDLDVNFDSEGATETVN"
     gene            complement(27772..29376)
                     /locus_tag="PKL93_03905"
     CDS             complement(27772..29376)
                     /locus_tag="PKL93_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNQ70437.1"
                     /translation="MERGRLILMLHAHLPFINHPDFPSFMEERWLFEAITETYIPLLQ
                     VFRRLKDDSVNFRVTMSITPPLMEMLANRDLQKKYINYLDSSIELAGSEISRTVSEHP
                     AANRLALHYLKELQEIKSVYEECNRDLVGEFAQLQESGELEIVTCNATHGFLPLMTNF
                     PETINAQVEIGVQSYKRHVGKDPRGIWLAECGYVPGFDSYLREHGLSFFFVDSHSFWY
                     GDPAPRYGVYRPVVTPESVFVFARDPESSQQVWSAEVGYPGDARYREFYRDVGFDREE
                     DYIRPYIDPSGERCNTGIKYYKITGKDVALNDKSYYDIDDARKATIEHARDFANRKRE
                     QVRRLSKQFDGEEPVIVAPFDAELFGHWWYEGPLFIEELFRELNGDETVVPSTPPEVI
                     ESMEQAQVVTPNTSTWGANGYNEVWLNGRNDWIYRHLDEGSRRMIELARKYERTEDPL
                     KIRALNQMARELLLAQSSDWAFIITTNTTVEYAVFRTKTHIDRLLTLYREIESDSIDD
                     HRLKTLEWIDSIFPYLDFRVYAKQVG"
     gene            complement(29381..30118)
                     /locus_tag="PKL93_03910"
     CDS             complement(29381..30118)
                     /locus_tag="PKL93_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HNQ70438.1"
                     /translation="MSNEEIQTFLSKDPTIQDLRRYAKMMGINLKRTMKKKDITRVLK
                     KFAQKSRQTSSTTTAKSSPSEILRPSKPVDAQIPSSYSRDFVVIHPVNPYWVYVMWDM
                     SSWTARSVSDHSSKLLVRVSDITNIIYDGKNAHRFKEANISPDAGNWYFQVDFPDADY
                     IAEIGYYIGGNFNSVLKSKITRTPRNSPKFSDYEIWVDLKKGSRKEYKASHEELVFER
                     ATKSSGPTDNPSSEEFIKTISKSKSGR"
     gene            complement(30253..31515)
                     /gene="hisS"
                     /locus_tag="PKL93_03915"
     CDS             complement(30253..31515)
                     /gene="hisS"
                     /locus_tag="PKL93_03915"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNQ70439.1"
                     /translation="MITRIKGTQDITFDDIGYWHYVEEAIRSISHRYSFTEIRTPIFE
                     ATELFKRSVGESTDVVQKEMYTFEDKGGRSITLRPEGTASVARAFIENSLVNLGAPIK
                     LFYNGPMFRYEKPQAGRLRQFHQFGAEILGSESPLADFEIIEMANSFLKELKVSKTKL
                     FINSIGCPKCREDYKTALRDYYRDKLPGMCTDCRRRFDTNVLRLLDCKIDKELAKKAP
                     SILDYLDEDCRNHFEELQWYLEKADIEYEVDPLLVRGLDYYSRTAFEIRSSSLGSQDQ
                     IVGGGRYDGLVEYIGGNSCPAVGFAIGLERIVMLLKSEKVNVNVPKIPQVAILAFGRE
                     ENIRGFDYSRRLRKMGISTYVDVMERNMRNKMKHAARIGAKISLIVGKEEIERDIVTV
                     KIMSDGSQFQVDSDYMSDYVVDKLRDIS"
     gene            complement(31520..33004)
                     /locus_tag="PKL93_03920"
     CDS             complement(31520..33004)
                     /locus_tag="PKL93_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNQ70440.1"
                     /translation="MFLNLLKQRYRLVFGYLGNLFVFFPIVLLIPLLYCIWNPEDFLQ
                     SLAFVEAASISFVIGMTLKLTMKVRPGSPISVQEGAIVVLFSWVMAIILSALPFIFAG
                     YFNFTQAIFESTSGWTTTGLTLADVETIPGTFLLWRSMMQYLGGAGIAIIMMSAILGP
                     AGLGLYKAEGRMDNLVPNIKSSTRTIATIYGSYAVAGIIMYRIAGMNLFDAINHSLTA
                     LATGGFSTKAGSIAEFNSVSVEIVTIVLMLLGATGFGIHYTIWKRNFRAFWKNGEPKL
                     MFVILAIFTPLLMTGTLSIFYSSGAEQFRHSVFQAVSALTGTGFSTVDFVPWNHFGIY
                     LVILLMIFGGDLDSTSGGLKQYRLFALLKILWLEIRRYFLPKDAILKEEIWKGETKRY
                     IDNSLVKEILLVFSLYFLTYAIGTGVLMAYGHSMAFSAFEYASALSTVGLSVGITAPN
                     APAGLIWAETIGMFLGRLEFLVVIYAITKLMRDGRILLRRRRNA"
     gene            complement(33005..33664)
                     /locus_tag="PKL93_03925"
     CDS             complement(33005..33664)
                     /locus_tag="PKL93_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNQ70441.1"
                     /translation="MKIVLIGGEKIAYHLARRFLTKGHNVMLVNKNERFCDEMAKKLR
                     AIIVRGDGSKRYVLEQLELDPTDLFVALTPYDQDNLVACLTASRVYGIERPVAMVNDP
                     DNKAVFEKMGIKSVVSPTEILGVALEDSLFREHITNLLPSSEKVSILRIEMTDKSPIL
                     GEAIKDIPLPDESVIGAIVREDEVVIPRGNTVIETGDVLLVLSSPTVQSSVFETLLGE
                     V"
     gene            complement(33661..34113)
                     /locus_tag="PKL93_03930"
     CDS             complement(33661..34113)
                     /locus_tag="PKL93_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNQ70442.1"
                     /translation="MPFMKGAIKKDEYYVVIFGCGRLGSRMANWLSSLGCSVVIVDRN
                     ESSFDALSNEFTGFNILGDATELDVLKEAKLDKADVALVLTTDDNTNLMVSMSAKEYF
                     EVPRVVARAYDPNNIKMFSDFGIEVICPTLLAVESIKSALYFIGGDDK"
     gene            complement(34117..34722)
                     /locus_tag="PKL93_03935"
     CDS             complement(34117..34722)
                     /locus_tag="PKL93_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ70443.1"
                     /translation="MALVYVVDDELNVRRIVKKTMENENNEVHTFVTAEEALEEIEKR
                     KPDLLFTDISLPSMNGIQFLKELKNRGYSIPVIVVSSDTSPESMRSAFKAGIVDFLTK
                     PFTPQEIRDAAELALREEDSIYKRAREIKRLIEEGETAKAESLMGNLFSDFPGSPFPH
                     FLYALLVAKDNPRVAIKHLKASLSLDEDFKDASDELKKLEE"
     gene            complement(34800..35195)
                     /locus_tag="PKL93_03940"
     CDS             complement(34800..35195)
                     /locus_tag="PKL93_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70444.1"
                     /translation="MKERVITFWLLFGGTLLATLSVNSFFGLLLTKREPFLAMIAGIN
                     SAIYFLGLAEKRSDLRKRYSHLLVGSFFSYVLSIYQVLVLFSIWLEGLLTSTCLLAFD
                     EMNLPLIISGFALSFLFDFAKKQFETNNL"
     gene            complement(35192..37141)
                     /gene="feoB"
                     /locus_tag="PKL93_03945"
     CDS             complement(35192..37141)
                     /gene="feoB"
                     /locus_tag="PKL93_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNQ70445.1"
                     /translation="MENQADAIESKKIKIALLGNPNVGKTSIFNALTGSRQIVANWPG
                     VTVEKRTGVMKLANHTVDVVDLPGTYTLGATSLDERIARDFLINERPDVALVIGDAVN
                     LERSLYLLLQVLELRGDVVLAVNAIDEARKAGFEIDKHELSKQLGIPVVLTSAVTGEG
                     IEELKNTLIKSARSSSHVHYLFTDEIEASLRNLSDRIKGNASLSAFDQRWLSIKLLER
                     DKEVEKLTGISLDYDYSIEIAETRYKYIKRILSSSVKGKEKIGWNLSEAIDHVMTHKF
                     LGILIFLAIMYIVFQLTFTISGPLSIMIENLLDGLGSVVGGWIAIDWLRSLVVDGVIG
                     GVGAILVFVPNIFILFLALGVLEETGYLPRAAFVIDKLMYAMKLSGRSFISLLLGFGC
                     NVSSIMSTRSISEPKERLVTILVSPFISCSARLPVYVLIAGTFFGVNAGIVVFFLYLL
                     SIVITVLSALLINKLFFKGEPSTLIMELPRYRKPRLTSIMLYTWNKGRHFLEKAGTII
                     LAASIVIWILTYFPTEGTGSFAATIGKALEPLFVPLGYTWEMITSLVFGIAAKEVIVS
                     SLTTFFGNPGVSGTSLDIMRTIISPEIALSFLVFVLLYVPCMPTLAVIKNETGSYKFV
                     FFSAFYSLAVAYAVALIVRVVGGLL"
     gene            complement(37150..37596)
                     /locus_tag="PKL93_03950"
     CDS             complement(37150..37596)
                     /locus_tag="PKL93_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNQ70446.1"
                     /translation="MVVPLSSMCEGEKGKIRKLELPPVTRERLCGLGFVTGEEIQLVK
                     VAPFGDPKVFRIKGSDITLRDDISMWILVETSSFPLSYVSEGDYWVSLINGGMGFRQR
                     LRMIGIEEGIKISVIGNLGKRIEVSAKGIRSVLSRGQAMRIIVRER"
     gene            38114..38917
                     /locus_tag="PKL93_03955"
     CDS             38114..38917
                     /locus_tag="PKL93_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNQ70447.1"
                     /translation="MHYIYQVDSFTAVPFSGNPAGVCVLEGQIDENLCLKISSEMNLS
                     ETAFVWPEGTSNTFNLKWFTPKTEVKMCGHATLATAWIQFTELGIKGEIFYETLSGRL
                     KVKLVDEMIEMDFPSEDPVRIDLDRRIGEIIGIEGNAYYSPGTEKLLYEIPVKDELET
                     LSFDTKRLLKTDFGKQSKGLIVTCSGDERYDFYSRYFAPWVGIDEDPVTGSAHTVLGP
                     FWAKKLNKKKLKACQLSARKGELETELLDDRRILIRGKAVTVIRGVISI"
     gene            complement(38895..39647)
                     /locus_tag="PKL93_03960"
     CDS             complement(38895..39647)
                     /locus_tag="PKL93_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70448.1"
                     /translation="MIFLALWALSYILGVILTEGSIAEHEGYLYRAVSRSLLWPIIIV
                     IHFQFVRKYEKADFDNVWLLSLYASFTFWIIFSAVGIKSIAMIMAIPISFLSAQFLRL
                     SKPSYGRILKHIKSSYLGSISFSFSLVILICSLSLFFSSEAASVLLLFLLLVPAIILT
                     HFKADLLSGGVFAWFSFAVGFVNLELSSYITVAGFSLGAIFLLLVSFSVFIVKEDPSR
                     KVFATVNPTEPIDEAELRIELDQEETRSKSRP"
     gene            complement(39710..41440)
                     /locus_tag="PKL93_03965"
     CDS             complement(39710..41440)
                     /locus_tag="PKL93_03965"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNQ70449.1"
                     /translation="MDYRDRYRKWLESPTIDEHTRNELISIKDDETEIKDRFYKELEF
                     GTAGLRGKLGAGDNRMNKYTVARATQGLANFILSGDPAYREKGVVIAHDSRHMSKEFA
                     EISAAVLAGNGIKVYLFDDIRPTPLLSFSVLQLSTVAGIMITASHNPKEYNGYKLYWD
                     DGAQVLPEIADEVYTQMRKTELFVGSKMIPLEVARKKGLLLEVGKELDDLYISKVLSL
                     TLRDSEDELDKSIRIVYTPLNGTGNIPVRRVLDERGFKNVFVVREQEWPDPDFTTVGY
                     PNPEDIKAFALAKRLGLEKDAHILLATDPDADRLAVMVRDDDDYVALNGNQTGALLVN
                     YLLLSMSEKGLLPENGFIVKSIVTGDMAKVIAEGFGVRTYEALTGFKNICGKALDIEE
                     RGEGRFIFGYEESIGYVAGNFVRDKDAVSTSMLLCEMAAYYLKAGKSLLDVLEELFAK
                     YGVFSEKQISIVLEGVSGQKRVERIMKEYRKSYPSEIDGSKLAHYLDFLSGVDTDIMN
                     GKESETGIPFSNVIKFSFDDGSWYAVRPSGTEPKLKIYIYSRSKNKEEALSKVERIER
                     IVLGRIESVE"
     gene            41629..42951
                     /locus_tag="PKL93_03970"
     CDS             41629..42951
                     /locus_tag="PKL93_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNQ70450.1"
                     /translation="MILLYRKIMVGENLESMQCEMYFESLFDVDFDYLVGLGKEMPTL
                     LFIDASKYSEDVLNDWIFAFTNIILINNNPIRAFIIRGYSEDRQEFKGYYRVLSSYKS
                     PRQIVQEVTTSIDENLDKIIGNSNRVVQMKKMIVLSMFYDGSIMILGETGSGKNLLAE
                     IIAKLSPRGSRPFYSINCAAIPENLLESELFGYKKGAFTGAAAEKVGLIEQANDGTLF
                     LDEIGDMPLNLQAKILAITENREFFKIGATKPIKVDVRFITATNRYDDAAIRNDLRYR
                     LSAVKVDLAPLRERKTDIPQIFDAILEKKGYLVKFENIPQDLKERFLSYSYPGNVREL
                     INMIEEYLAVNDVVQASVNSSMSKNVKLLAEEAMVARMLNGTTYKDFLQEVYKSVSHE
                     LLVQRSEVLGNNMNELSKEFGLTTRRIRDLLAEANSYIKDQSTEVDDE"
     gene            43022..44002
                     /locus_tag="PKL93_03975"
     CDS             43022..44002
                     /locus_tag="PKL93_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNQ70451.1"
                     /translation="MAVRKLSLPMGVEVAELFGEYDRKAKYIQKKLDVRISIIGNEIW
                     IKGEDGNGIDIAEKIVDELVQMSRDGHLVEWREFEYIVDQHLQHDEDDDSFKGVYNEV
                     KETTLLSNRIRPKTIGQKNYMETMKERELVFSIGPAGTGKTYLAVAMAVDFLKSGKVQ
                     RIILTRPAVEAGEKLGYLPGTLLDKVDPYLRPLYDALMEMIPAEKLSYYRDSSIIEVV
                     PLAYMRGRTLNNSFIILDEAQNTTYQQMKMFLTRIGFGSRVVVTGDITQIDLPRDSGS
                     GLVVVQRVLKSVATIGFSYLTDQDVVRNPLVKDIIKAYDEYEREEKTEEK"
     gene            43965..45458
                     /locus_tag="PKL93_03980"
     CDS             43965..45458
                     /locus_tag="PKL93_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNQ70452.1"
                     /translation="MNMREKKKLKKSDLSKVQFPLERLLNIKDLTNFIVFPVFLALLN
                     RLPFLSLPSKPFLYFVGSAILWYIFAERSVRSNRYFNLHSAYRITFFSVVSFGVFVNS
                     FVPSLGDETLLPEMTPIFLSVSMVTILIGYQEGIGSGLFMSFLASANLDNSLETFILL
                     SVVVLATALTTRAIERRIEIARAGFEVSLIFVAINAIRMSLQGESINAVDLLIAFANP
                     ILSSVIAIGIIPYLEYASRIYSNTGLLELGNLSHPLLKNLSIYAPGTYYHSISLANLS
                     EAAAERIGANAILARVASYFHDIGKSKRPQYFAENQQEGMNPHDNLTPSMSNLVINEH
                     VKQGVEQARKYRLPLLVEDVIKEHHGTRVKKFFFHKAKNIGQEERSDDFRYPGPKPQF
                     KESGIIMLADSVEAAFKSIKKPTPAKTQELVEEIVNGIYNERQLDQSGLDLDDLEKII
                     EAFTRVLMNMNQPRIEYPKETTEKVVSINGDSNSKQDIEEDRREQNI"
     gene            45400..45828
                     /gene="ybeY"
                     /locus_tag="PKL93_03985"
     CDS             45400..45828
                     /gene="ybeY"
                     /locus_tag="PKL93_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNQ70453.1"
                     /translation="MEIQIQNKTSKKIEESRISNIVEKVVKAELGERKIGMLNLLFTD
                     DSEIRSINKEFRHRDEPTDVLSFQYGLEEEIVGDIVISLERISEQAGEFGNSFEEELY
                     YILIHGVLHVLGHTHEGYDDEGEEIFSLQRNYYKRFVEEG"
     gene            45832..47073
                     /locus_tag="PKL93_03990"
     CDS             45832..47073
                     /locus_tag="PKL93_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNQ70454.1"
                     /translation="MSDFRPFRAVRPAEGIEQKVNCPPYDVVSFEEAREIGRNPESFM
                     HVIRAEIDLPVDSDHYSDLVYEKARENFETMLQSGVLVQEEEPLYYIYWQKMKDHIQI
                     GVVGGASVDEYQKDLIKKHELTRQDKEEDRIKHIKTVNANTGMVFLTFRSNEKLETLL
                     QSYSRKLPLSMRVIDENDVEHRLYAVKERDGVEEIREAFKEIDCMYIADGHHRAASSS
                     RVRELLKKSNPGHTGNEEYNFFMAVAFPHTHLKIMAYNRVVQDLNGMSENEFLEKVGR
                     VFEVENAPKDPYEPAMRHEFGMCLMGRWYKLTAKKGTFDENDPVSSLDVDILQKNLLS
                     PVLGIDNPRKDSRIDFVGGIRGLKALENRITNGWAVAFSMFPTSMEELLAVADEGKIM
                     PPKSTWFEPKLRSGLVVHLLK"
     gene            47070..47756
                     /locus_tag="PKL93_03995"
     CDS             47070..47756
                     /locus_tag="PKL93_03995"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HNQ70455.1"
                     /translation="MKTGAIIVAGGTGRRMKSSIPKQFMTLGGKEMFVRSVEIFHGSD
                     FVYSTVLVVHRDWINKAKEILLKRGLNSVMVVPGGKSRQESVYKGLLSCREVGLEAIM
                     IHDAVRPFFSRSQIPELLRLVAPGRGVVVTEGVNDTVYLVGNDEVLDVPDRNCLRLAE
                     TPQTFPYDEILEAHKRAVEENILSSTDDAQLFVRSGGQVITVNSIAPNPKITTPFDFD
                     MAELLINKGS"
     gene            complement(47779..48948)
                     /locus_tag="PKL93_04000"
     CDS             complement(47779..48948)
                     /locus_tag="PKL93_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70456.1"
                     /translation="MKTKGGYSTIVAIFGILMGVIAILNYLFPLNHMPLPDGPHNVGF
                     RSYEVSNPQPMSVAGIDYSDPGKIRLEVWYPAGDVSTAPRKGWIDNDPLVFQGFEIMT
                     GYPEELFEHIYRVKTNSFIDAPPVDDSSTWPIIILLLGWSSISELHTSLAESLASAGY
                     LVVGIEHPGACAVVSFSNGDVHYFLGNDLLERLPEGTRESKVRMLSEYLARDINYAIE
                     YLNGMNEDPFSDFFERLNMNEIGLYGHSGGGAVAIQYALANKNVPMVLADPTLEGFSV
                     QELDTAFSNKILLMSTEEWEHGISEEYSSTIAKERNESLYNLRICGMRHVDFAMVRNL
                     SPLTFFFGETGRFMNDEDAIGYLDSAVKLFFDSVFFGGEIEELFALSESVPEFSM"
     gene            complement(49635..51458)
                     /locus_tag="PKL93_04005"
     CDS             complement(49635..51458)
                     /locus_tag="PKL93_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ70457.1"
                     /translation="MNQAWERKARVLVVGDVFLDKYVYYDPNLSRPSLETGLMTITGV
                     REEYSPGAAGNVAKNLATLDGKVILLAPVGLDARAVELERELDRYGICTQFLVKSERQ
                     VTPLYMKFINALTGKEDLPRLDIPPAGLVEENRRKIVEMLEFLVPQVDAIIVQDQSEI
                     PGTGCIDEEVRKKLIVLRKLFPEKLFVADSRNWLEAFSGFLLKPNLGEFSQILERAGL
                     VDDNNRCPDQILVQKHLAEVSKLVGSPVVVTASEDGSFCFNNGILNRIFNLEGEVRDV
                     CGAGDAFIAALTLELCGKKVSLLDSAKTATKAASLCVSQKGTGVLLRESISKLEEPNA
                     YRVEDPSIFRNTRRLPGKTRYVLFDFDGTISLLREGWQPIMRDLMIHFITGDREIEEE
                     TRKKIKEEVNDFIAETTGLQTILQMEGLQRLVIDYGFVSEKEILTPHQYKQVYTGHLK
                     DIVRKRINEDSRDRYLVCGSLEFLKSLHERGITLLVASGTDLEDVIEESKYLGVYDFM
                     GGGVYGALSSFEEYSKKKVIQRLLTDRKLQPDELIVIGDGPVEISVGKEAGAFTIGVA
                     SDERKGFGWNKEKFNRLKRAGADVLIPDFSPLSALIELIFP"
     gene            complement(51455..52456)
                     /locus_tag="PKL93_04010"
     CDS             complement(51455..52456)
                     /locus_tag="PKL93_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70458.1"
                     /translation="MRYEGKYTVFDTSNVKVYSLLDRPSEVSISDLIDMKEIVNESDG
                     FRSPELEELSGKVLEAKEEGLPVIVFSGAHVIKNGMGPLLSSLMHEKIITALGVNGAF
                     TIHDFELAYCGRTSESIPNALSRGLFGFAEETNKYINNALIHGNKLFLGYGESMGRLL
                     SGEAFPEKIDCMYREVSVQYNAYRDSIPLCVHSAMGTDIYHMTPYFDGESLGGCSARD
                     FLIFANEISKLEKGGVCIVIGSAVIGAEVVLKAISMAANVGRAPKSITTASFDIRDAD
                     LDEASKNNKEVASYYFRDVKSIVTRIPNAFGGKGYYIKGNHKETLPSFYRLIMKGML"
     gene            complement(52488..53069)
                     /locus_tag="PKL93_04015"
     CDS             complement(52488..53069)
                     /locus_tag="PKL93_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ70459.1"
                     /translation="MEFNRVARMAFEESMRVKSEFIDKYEELIYEVARKIARRTGNGG
                     TVYLMGNGGSAADAAHLAAELVGRLYLERRPIRAVSLPSNDSILTEIPNDFGYDQVFS
                     RQLEAFLAEEDIVIAISTSGQSTNILRGLKVAIERRSLRVGLTGKTGGEMLGLCDYLF
                     KVDSTDIPRIQETHITLGHVLCQLIEAITVGRE"
     gene            complement(53073..54002)
                     /locus_tag="PKL93_04020"
     CDS             complement(53073..54002)
                     /locus_tag="PKL93_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ70460.1"
                     /translation="MELIEERTLLGIDVGGTKTAVVLGDEALNILARAEFPTEPELGF
                     QSFIERLSSEIIGLTGDLIPSRVGISVGGPMDCKTGTLFNPPHLRWGTVNIVQPLVER
                     FGFSITIEHDGRAGALAEGILGAGRGFLNIVFLTLGTGLGAGIIINGNIYSGSTGLAG
                     EVGHMRVAEDGPSLYGKNGSWESFCGGTGISLYAHYRFPERFGRGTTTQDISHLALKK
                     DPNAITVLEESGAYFGKGLAVLLDILDPDRIILGNLAWRLPKIWLEAALKRAAEEALV
                     GDEARNRVVITELKDRIGDYAALIVASGMGRRR"
     gene            complement(54025..57141)
                     /locus_tag="PKL93_04025"
     CDS             complement(54025..57141)
                     /locus_tag="PKL93_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNQ70461.1"
                     /translation="MYHSRNEYCTRLRKELAELFPYTLIERKAIGGWRFSDFGAERPP
                     EKGWKEISVGYRWDETKFPVWFRTSIEPEKPQAGERLFLKAVPGGESLVLIDGRSFGE
                     INVYHQFLDLTEFADGKSHVIDIQTVPKGLFGTHLPDPVFEGAELLLQDRFVSSTYLD
                     FTVAIEVLEATRDEFLSEKLLKIISDCLSEIELPRSTDQYFKTAPDNPTLFGHHTYGS
                     VNEIWQSPRFEKSEGNRLTDNYRHTVIKAREKLASNIASLRDEHSAVGTVHVTGQSHI
                     DYAWLWPIDETKRKIRRTFANAVRMMEKFPEFVYLQSSAQMYMDLKENDRDLFERVKE
                     KISQGKWETIGGMWVESDCTISSAESLARQFLYGQMFFEKEFGMRSSVAWLPDVFGFS
                     WILPQIMKEAGIEYFSTTKLKWNEKNRFPLDLFLWRGLDGSEIVCHFFDNPLGGYNGM
                     LNPEAILGTWENFQDKRVYDKTITTFGYGDGGGGPTDDMVEYYERLKEYPGMPKLEMV
                     PLTKYFKELPSTLELPVWDDELYFELHRGTYTSQARTKKLHKDAEDTLYRLEVVGAIV
                     CSREKYPLERARKLWATLLHNEFHDILPGSSINEAHIQAENELGQLIREANQITGTLL
                     DSAGDGNPSFKTVINVGSYPQKSIFIDDNEMNLYDGTGKLLASQRTYDGRFVYSYDHD
                     VQPFSSEVFECIGGEVEEHAMESDPVLENSFLRVEVNSDGSVSVFDKQLQKEVTSGPG
                     NCLYVYKDIPVAWDAWDIDHDYKISGKRLTALDVRTIESGPVRKVVQTRYEYEGSSIV
                     QRYVLSASSRRLDIENDIDWHTRRTLLKAHFPVSILSREVRCDLSAGYSRRSTRVRNT
                     FEDARFEFPAHRWVELEEPGLAVAILNDGKYGHSCNGSDISLTLLRSPIYPDFFADEG
                     RHRFTYSLFSHDGEDAVRVVEEAEGLNKEMIKINGKIDLGTAVIQISARNLKVLALKH
                     SEKSGYVLRLAEVEGRRGVAKISTALAFERCWISNVLEDKVCELPVIDGKVELPYRQF
                     GLHTLRFE"
     gene            complement(57203..58357)
                     /gene="aar"
                     /locus_tag="PKL93_04030"
     CDS             complement(57203..58357)
                     /gene="aar"
                     /locus_tag="PKL93_04030"
                     /EC_number="5.1.1.1"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-alanine/L-glutamate racemase"
                     /protein_id="HNQ70462.1"
                     /translation="MEKKYITEILHHLGEDDLPCGAVNPPIFQTSIFSFKGFDEFSTA
                     LSDETNHFLYTRGNNPTVNILEEKLAALEHAERAKLFGSGVAAIVSSISAFLKSGDHI
                     VTVKDCYSWTSTYVTQYLARFGVEHTYVEGNDVNEIEAALKPNTKIIYLESPTTFTFK
                     LQDLTEVSSLARMRGVKTIIDNTWATPIYQNPIDFGIDLVVHSASKYLGGNSDVVGGV
                     VAGSDEDVRRIFEKEFLNTGAVPDPFAAWLILRGIRTLHIRMPVHFQNAMELSRRLQK
                     SEAVESVLYPMLESSEQYALAKKQMRGGSGLFSFKLKTRDIDRIKKFVNSLRFFRRAV
                     SWGGYESLISPYAVSHRDPGDNVSLIRIHAGLEEIEVLSEDLDRAMKELG"
     gene            complement(58377..59216)
                     /locus_tag="PKL93_04035"
     CDS             complement(58377..59216)
                     /locus_tag="PKL93_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70463.1"
                     /translation="MKTVMKVLGKVFKVIALGLYLIAALLPLYWVVVTSLKGPREIYT
                     FPLKYLPSKLSFESYSKLFGFANFGVYFRNSAIVALAAALAAMLISMFSGYALSRIRA
                     KRFRNGTLLAMYFTQMVPPFILMAPLFVMLANYGMTDRLSTLFILYVTMVIAFSTIMS
                     KSFFDRIPVSLEEAAVIDGCNTLQALFKVVFPLTRPGLAAIFSFAFVNIWNELFLASM
                     FIFSDEKMTVPVALNSFISKAGISWDVLSAGLVVSLLPTMIVFAFAQRYIIAGLTEGA
                     VKG"
     gene            complement(59213..60097)
                     /locus_tag="PKL93_04040"
     CDS             complement(59213..60097)
                     /locus_tag="PKL93_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70464.1"
                     /translation="MRKNFWKFFIMMIPAFILLIVFTYTPIIRGGVMAFQRYNMFDLS
                     DTGFIGFGNFKAIITDRNFDFVRIVINTLVWVFFSLIFQFGLGFGLALLMRKPFKGRG
                     IYSALVFYPWAVSGFAIGLIWAWMFNGQFGIINDILVRVGIISNPIGFLSDPKFAMMS
                     VIIANVWYGIPYFAIMLLAALQSVPNELYEASRIDGANRFKQLFKITIPYIRPTIVST
                     VLLRTMWIMNFPEIIYGMTGGGPANSTQILATHMINRIYQFYDYGQGAAIGFIIMSML
                     LLYAIVFLNISSRKEVTV"
     gene            complement(60217..61467)
                     /locus_tag="PKL93_04045"
     CDS             complement(60217..61467)
                     /locus_tag="PKL93_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ70465.1"
                     /translation="MKRKVVLSLLLVVLIGVSSFGVVTLNFIEVLTSPARTQVIKEII
                     ADFEAKNPDIKINLISPPYEQADQRATLMLNTNQPLDIIEVRDYTVKQFVNNGKLENL
                     ESYLAGWEHNDTLTFVANQAARTVDNTAFIVPQFFFIKGLFVRTDILEKLGVSYIPKT
                     MSELVELCIDITDPEKNQYGFGFRGKSWEFKFSDLIATSFLSDIDATNIYKTKSGEAY
                     FDDPRAIEGLKMYVRLFEGGVPADGINWGFNEQVNAFVSGITPFLIQDPDTVALVDEM
                     LGREYYTVVPIPVGPSGKAYLDYGFSGLGIPSYSKHKEEAWEFIKYISSPEVNAYFSK
                     SYGPLPIHSSTYENDPYFSSGVYTAWSYMMSHPEKYIFASYPLDSEKWPGWPEIHQQD
                     MQSLLLGDTTVEAVAAKWAEYWED"
     gene            complement(61498..62649)
                     /locus_tag="PKL93_04050"
     CDS             complement(61498..62649)
                     /locus_tag="PKL93_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNQ70466.1"
                     /translation="MITKPGDLREKNMGRVIQLLLANPDISRVEIARKTELTKTTISA
                     IIAALLDLNIVEETPGLKTGLVGKTPIPMRVRGDAAVAIGVNLARGSTSGVVMTACGK
                     LLESVSKEVSNLTRADATAHLFEVVQRLLNKCFRNGIDVDAIGIGAPSPLDKEKGVIY
                     APYGLSDWKDFPVGEITREKFGLPVFLVNDADGAAFGMKCFGKCRDIRSFISMYFDEG
                     VGAGIILNDEIYSGSSGYSGQISYALIKSFCNGQVENSRFSLDTVVSDLNKEIGVNCR
                     KIDDFLDRRFNGGQKMNSVLSRVGDELGRIAALVSNIVGPEAVVVNGRLLDFGKVFFA
                     ALKDSFESNLFSRGSVQLIKGFTNQFRAFSTGAASYAIFSYLMDKVLSN"
     gene            62945..63844
                     /locus_tag="PKL93_04055"
     CDS             62945..63844
                     /locus_tag="PKL93_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ70467.1"
                     /translation="MNLTMSCFMCSRRRGFSVIEMLIVLAVLAGLIAVIAPIGVNTLR
                     KSQAVSVAKDLKTLSSVFSSKIYLDGGVPVVIGELGRNVDGRSFGAAWKKNDDGEYNY
                     FVFTNRSVDFETVSGVLVDSRQGIPFGIDDFTFLDGGLGRESLDSETIYYSLLGENSI
                     APLTEFGSTFNEISEGLKTLIQEFFVNNGRYPRDWIDKESGKDYRFEDLSLDASFWAN
                     SAFGGVVYKPAGKLLRVSPDNGYKFVFNFLGSSEKGELTERLKWDLIYNIGNDSADTG
                     CWYFHSLEGRKIDISTLEIVKLS"
     assembly_gap    64764..64863
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<64864..66266)
                     /locus_tag="PKL93_04060"
     CDS             complement(<64864..66266)
                     /locus_tag="PKL93_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HNQ70468.1"
                     /translation="MYGRAIEFPESSKWINVERPLTLADLKGYVVLLDFWTYCCINCI
                     HVIPDLKWLEKKYEDSPFVVIGVHSAKFENEHDERNIRAAVQRYEIQHPVVIDNDYFL
                     WERYVIRAWPSYVLIGPDGKILSKISGEGKREYLSHEISKALEEGKRKGVLSEKRIPV
                     KLELTKRESNLSFPGKIAFGDGENLFISDTNNDRILLTELSTPFVAKTIDQIGSGQSG
                     LEDGPFENARLNKPQGIVYSNGRLYVADTENHALRIADMNQRCLSTLSGDGFQDNDWN
                     YNGDASKARLNSPWDLQTDGRFLYIAMAGMHQIWRLDLENNTISAFAGSGVENIGDGH
                     LKDANFAQPSGLFLDRNSLYVADSEVSAIRFIDLEAGTAQTVAGSGLFSFGYVDGILK
                     RSLFQHPIGIHGEGRFLYVADTYNHAIRKIDLGIRRVETVIKNLREGTCTLNGDKCSS
                     LGLFEPNDVKFHKGLLY"
     gene            complement(66557..67600)
                     /locus_tag="PKL93_04065"
     CDS             complement(66557..67600)
                     /locus_tag="PKL93_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ70469.1"
                     /translation="MKQVADYAGLSAATVSRVLNGSRPVQEDLKVKVWDAVKKLNYKP
                     NVGARFMKGQTTNVIGMLVPDVSQPFFSAMIAGAILKAGEIDHVIIISTSDGKRELEK
                     AAIDSLSRTVVDGLIYCPVSTGDPLPQIESFKELPIVVVGRRNVFKSKPHVYTDNVKG
                     GYLATKYLLRLGRKRVGFLAGFWSSPCTIDTIGEVASSEDAGKYTTLDRYMGYRRALE
                     EEKIEYDPSLIVICGYDYEAGYIGARELLERMVEVDSVIAANDLVAAGVIGFLTDQGI
                     NVPDQVSVVGYDNNLIAPITIPTLTSVDQDPRTLGAESVVALSKILSGKEVSDTIIDV
                     KLTIRNSTSVMHK"
     gene            complement(67621..>68121)
                     /locus_tag="PKL93_04070"
     CDS             complement(67621..>68121)
                     /locus_tag="PKL93_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ70470.1"
                     /translation="TASLWSTLISIGVMVGNFVGAYIARPARLRLPAITVSCVSYGAS
                     WVFFWFSMRSRLPILIPGLVGFFLGFFAGITYDHLTAGVNDLAARGRGGSLFGGMNLF
                     TFISVIIFQSGTGFLMQRFSSSGGAEIDLAAFNSTFGIVTLLVIISLVALPFLKSFSG
                     QGGEVL"
BASE COUNT        18343 a        16394 c        14816 g        18368 t
ORIGIN      
        1 agagttttcg gcctatgggt gggagcccag atgtttgttt acaacatcgt tctctttctg
       61 ctggccttcg tcactctggg tgtgggtttt gggatgaagt cgatctactc ggccgttctg
      121 atgtcattga gcgtcgatct gctgcagaaa ctgagttttc ccgtgtttga tgcttcttcg
      181 actcaagatg gattcctgct cgtagcaatc tacggcgggg taatcgccgg ggctggaatg
      241 gggctagtct tgtggcgcgg tgcttctaca ggtggtaccg acattgttgc aatgattctc
      301 tctaagtacg tggggttcag caccggtacc ggtttgctga taagcgattc acttatcaca
      361 cttctagcgg ttgtggtttt cggaccgata gctgccatgt acgggattat caccatcttt
      421 acaaccagta agactataga cgggataatt gaaggcattg gaaacactag aacggcattc
      481 gttattagca aagagagtga agcgattaaa gagaaagtga tcaacgaaat ggaacgcgga
      541 acgacaataa tcaaagctac aggaggtttc tccggagagg atagaccggt tctcatggtt
      601 tcgataaggc ggagagaaat cggacagttg cggcacattg tcaagactac cgacaggcgg
      661 gcctttgtgg tagtcgtgaa taactctgaa gtcttcggcg aaggcttcaa ggatctcagt
      721 tgaggcaagt tatgaaatac atctgtttcg atacatcgtc aagaacgctt ctcctttctg
      781 ccgtcagcaa cgcggcagag atggatatca gatgccgtga cattgataga tacggttcta
      841 tgctagccgt aataatgaga caagccattg agaatctcaa gatcgatgtg tccgaactgg
      901 aatttgtcgg agtcggaata gggcccggtt ctctcacagg tttgagggtg ggaatctcga
      961 cggcaaaggg tctcgcattt cctcacggta ttccagccgt accattcaat tccttggatg
     1021 tgctagccaa aacagttgat agtgacagat atctgatttt gagaaaagca agggaagacc
     1081 attactactg gagggagtac aaagggggat tgcctgttgg taacactggt ttcagcaata
     1141 caggcatgat caaatcgaaa atcgagctgt cgactatgga gcttttcttc gacggcgaga
     1201 tcgatccgga atttgaagac ttcagatgta gcctggtgaa gaattacgat ccctcttcaa
     1261 tgagggagat agttctggac acgtatgctt cgggtaatca agttagctgt tcggatcttg
     1321 cgccgatata tctgcagaaa tcaatagccg aaatcaactg ggagaagcga agtaacgaga
     1381 aagagtctta gggttttctc aggaggatca gcgggacgta catctcctcc tttgttaatc
     1441 ccccatgtct tcccttgaga gagtgttcct gcccagtata tttgaagcat agagaagagt
     1501 tccccttgga aaccgttatc aattcacccc tcctctttct gaagacactg ttgagaggtt
     1561 ttccaaagag ctttatctca tccagtcccg acggttcaat aatctcaagt gcatcaccaa
     1621 actgtctctc cattttcctt ttcgttttct cgccgtcata tgagtagata tatgacatcc
     1681 tttgctcacc accgggcatt gagtacatct cttcgaagag atcatcgaat ttcgaccacc
     1741 atatttcttg ttcccaggga gtctctattt ggccgtgatc agcggtaatg aagaaagccg
     1801 tattgcgttc ggctatatct tcgaaaaact cttcaattgc tgaaataacc gttgcagttt
     1861 cagatgtgta agatagggag ttcggtccgt acctgtggcc gtaagtatct attaatcccc
     1921 agtagaccag cgtgacactg gcagcctctt ccgagaaaag ggccgttctc agctcctcaa
     1981 acatatcgcc gaggccgtga acaccccggg ctatgccgcc ccgcgaatgc atcctcgtga
     2041 gacctgtgtt gacaaagctt ttcgaagtga gatggtaacc tcttactccg acttctttga
     2101 gcaattcgaa gatcgtgggg ttgggattgt actttagggg atcgaatcca agtctggtca
     2161 ggttatctcg gtcttgggtg tacggggcta gctcaatcat gttgaccagt ccaccgtatt
     2221 ccgggacatt cagaagatag cccagtactc cgtgttctcc gggtggtgtt gcagtgaata
     2281 tcgatgtaag tgctgtggca gttgtggaag gaaagacgga ggttaaaatt cgtatatctt
     2341 tctggtcgaa atatttgaag cttttcttct ccagtacctt aattagattg tagtatccca
     2401 ccgcgtcaat aataaagact actactttgt ctattccctc aaggctgagc ccattatcct
     2461 tcaaagagta tgctttgacg ccctgaggaa tgtcggcgtc aaaatgttcc agaattgcgg
     2521 ctgagaaatt gactatcgaa ctcccgtcgt atcgaggaaa gttcagctcc attgaaactc
     2581 ctttctatcg agggtagaca actccggtac ggaacgatct acactcccca ctccttacca
     2641 accatcacaa tttgtcaatg ctgttcatca atgtcggctc aatgaaatta tatacgacca
     2701 cttgttctac gaacaaaaaa agaaaacctc tctgaacaaa ctttcgaaaa aactgattct
     2761 caaaagagcc cgccccgctc gatatagcgg gtgggctccg aaagagcgat aaccttgaga
     2821 aatctatgaa ataacaatcg aaaaagcagt ctttactcct taactccgcc ctgagtaaga
     2881 ccacctacga tgtaatcctg aagaacgagg aagaaaatta ccatgggaac cgcgccaatc
     2941 agtgcagcag ccgtaaatgg accccagctt gtttcgaatc ttccagaaaa ctgccagaga
     3001 ccaactgcgt atgtccactt gttcatatcc tgcagcagta ttctggcgat caggtactcg
     3061 ttgaagatac tcatgaatgt aagtattgca actacagcaa gaattggtct tgcaaggggc
     3121 aggatcacct ttgtgaaggc ctggaacttc gttgctccat ctatcatggc agcctcttcc
     3181 agagaattcg agatggtatc gtagtaacct ttgatgagcc agatattgaa agctatccca
     3241 ccggaatacg cgaagatcag gccgccaagc gtgttgagcc ccagaaatgg aatcaagccc
     3301 ccaatgaact gaagaagagc gtacaatgcg accatgaaca ttatcgtcgg gaacatctga
     3361 attaagagca accctagaag gccctgtttc ctcccacgga acctcatcct gctgaaagga
     3421 taggccgcaa gagcggcgac ggcgacactt atcaaagaaa cggaaagggc aacaatcacc
     3481 gaattcagaa gccacctcac gaaatagaag taggtgctct ccttctgtac ccatatctga
     3541 ttagccctga aaagggcttt gtcgatctcc ctcaattctc tatcgtaaga tgccgagtaa
     3601 gaggctggtt tctctatcaa gtctgatatt agcctggaag cacgttggta cctcgcattg
     3661 acctgctgaa agagcgaatt ataggtctcc tgcatcgttc cgaagtcgtc taacgtcaca
     3721 gtcgtaccta aaaatctaag gtcgatataa tggttcacct ttgaactcag ttcttctttc
     3781 atatctctca tggttgaaat taactggttc aacaaagccg ttccatcccg cgcttctctg
     3841 ttcgccctgt cgaagtcatc gtaccaacca agtgttctat aacgattcga agcccttatc
     3901 ccaaagtacg tatcgagaat atccgtcgtc tttgagacat aagcccttcc atcttcaaga
     3961 gaggcaattt cctcttcaac cagagccagg gtagatttta ttcttatgga ttcaagcgcc
     4021 atcaagtatt cagcctctgc tctatcggcc tcttctttga gctcagcaat tctgctgccg
     4081 agtatttcac tcctctcctc aagagatttc agttctttcc tcaaagaggt cgtgttttcc
     4141 gaaacttcga cgtactcaga aatgcttgtc tcgagaacgg gaagaatcct ggaaagattt
     4201 gaaatagcga tgtttattac atcgatcccc tttgacacct cagagtttcc cgaggcattg
     4261 gcaagggcct ctttgttatc ggaaaaccag gccatttctc ccaaaagctc tgaaagacta
     4321 gaagacagct cctgaacatt ctgcatctcc gcgagctgct gttggagaag accgatggtc
     4381 tttcttgctg agtcgacctc atcgagtgaa ctgttaagcc tggcttctga aacagccgtg
     4441 tactcttcga ctccttttga aatcacaatc tgcaaagggg gaatcaaggc cgaaagcttg
     4501 ctcttttcgg aaagtattgt gggaagcgcg acctcaaacg aatcttccag ctccgccaat
     4561 gcagattcag aagtcgccag tgccttttct accgcctctt tatctgcttc tacaacgcca
     4621 agttcttcga tcgcggagac atactcatga tactctggac caaaagcctc ttcagcaagt
     4681 aaagagaggt cttgggagac tttttcggct gcagcttcga cttcttccca ggcatctcct
     4741 gccgccttga aggagtcgta gagcttcgtt cttactccgt tcagcgcatc ctgttctgcc
     4801 tggggcattg tcgattccac atctcgcaaa tatctgttaa ttaccctgag ccactctact
     4861 cttcgccatt ccgagtaatc gattgaagag agtgatatgt aattctctat cgactcaaga
     4921 gatctgacaa cttcgtcttt gttctcgaac tccagttctt ttattgcgtc gagcgtctcg
     4981 tccctataga gaaagacatc ttcgtatagc ttcctgtann nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnntt gcgtcgagcg tctcgtccct atagagaaag acatcttcgt
     5161 atagcttcct gtatgtctcc aaagatttca ctatgggatc cgctctttca cctaacaaat
     5221 cgatgtagaa gggatagact ccttcgagcg aaggggcagt tgtctcaagg agttcgccga
     5281 cggcgtaggc catcttcact tcgcttagtt cacctatctc ctgcaatctt tcttcaaaaa
     5341 gtgctttatc gcgcagtctc acctcgttca tctcacgaat gatcaaactc tctttttctt
     5401 tcagctcttt ttctatggct gaggttccgt tctcgatgga agcgaccatc tcttcgcttt
     5461 catccatgag atcctcaaac ttaacaagat atctctctac agtcttcttg aggtcctcct
     5521 gacttcttcc tctgtattca ccagtggcat atgtggcgct ttgcagatca agaatcagtc
     5581 ttcccacact cctctctgga aaaagaagat ttctgtagtg ctgaaaggta atcctgcttg
     5641 aaaaaagatt agatgaaaag gctgcctgat ctctcctaac agaagtgctc acaagccaga
     5701 ccatcgggaa aagaactaca acaacaacga caataagaat taaatgtctg atccagtatt
     5761 ttcttctctc aacggccatt atctactcac ctcttcaaag gagccggaaa gtttgaagtt
     5821 tatccagctg aaacctgcga cgatgatgaa tattagtatc gatatggcgc tcgcgaaacc
     5881 aaagtcttgg cctctcccct caaacgcgag tttgaacgta tacgaaatca gaatatccgt
     5941 agcccccgcc ggcgttgaag ctcccacgat tgcaggccca ccctgggtta ctagatagat
     6001 gccggtgaag ttgttgaagt tgaaggcgaa gcttccgacg agtaaaggag caacggtaac
     6061 catcagaagg ggaaaagtga tctttctgaa tctctgaaat ttcgtggctc catctattga
     6121 agaggcctca tagaactctt ccggtatgct ttgaagagcc cccagactga cggtcatcat
     6181 gtaaggaaat ccaagccatg tattgacaag caagaccgaa acctttgccc agaaggcatc
     6241 attcaaccac tttattggtt caaggccgaa gagtccctgg ataatgaatc ggttcaatac
     6301 accataggtc tcgttgaaga aaccatttcg ccagactaga gcagatatga atgcgggaac
     6361 cgcccatgga ataatgagca gagttcgata tatcttcttt cccttaagtc tcttgtcatt
     6421 gagcgtgatg gcaagagcca atccaatagt gaaggaaaaa agaacgctaa gaccagccca
     6481 ggtaaatgtc cagccgaata cctggaagaa aggtccgctg atctttggat ccctgaacat
     6541 tctcacgaaa ttccagaaac ccacatccga tatgtaaccc tctacggcga actcatttcc
     6601 gttctcgtcg atatcgtaga agaagccgcc ctcttcaacg agagtcctgt ttgtctgacg
     6661 gttgtaaact accgtctcct gtacggccct tcccgcatcg aaggtagttt ccgttctcaa
     6721 cgcatacttc ctctcagttg tggcaaaggt tgtgtaaagt gagttaggga acagcctcac
     6781 ttgatttcct tcgctgtcag taaactcggc gttccgaagc caaatgcctc ttatctcact
     6841 gaaatagaag ggtgcattcg gcctcgtcga gtcatcctga ggagagtaaa aatagatgaa
     6901 acgttcaccg gaggttgccc tgattgcaag aatcttatcg tctggtgata tagagagtgt
     6961 gtagctgact ccgcccgtgg aaaaactgtc gcccgatacc tgaaccatct tggcagttgc
     7021 aagagtgata tttccttcgg catctttcgt tactggctga ggacggagcg ctgcaaatag
     7081 agcaccggtt tctttggact tcagaagagt tatgaaccta tcagtcggct tgtagtcatc
     7141 aagctcaatg aagattgaaa actcgaactc ctcggggctt tcaggaatgt agaagtagtc
     7201 actgagaagc ttctgtatag attgctgcct agtgaataaa tggccggtac cgtagtttgt
     7261 gaaggcagtt ctgaacgtgt aatacattgg gtaaagcgta agaatgaaca gcaggatcat
     7321 cgcgggaatc atgtacctgt aaggataccc ctttggattc agtgcaataa tatcgatcaa
     7381 caaggttagc acaatcatcg taaccataag gccaaaattc ccgtctgcaa aaagcagata
     7441 cacgctccat aaggaaaagg cgttgaatat tgcaagaatg gctaaagaaa agagaaagat
     7501 tacaaatcgt tttctactca aggtaatccc tccgggcaat tagaactaga gcgcgggaag
     7561 tacccgcgct cagcctaact aagtttagag atgtgaataa aatatggcaa ctaatttccg
     7621 atgctggtct ttatcttttc aaccgccgaa ttgaaagcgt tttcaacggt gtcttgaccg
     7681 ttaatgatta aaccaagtgc atcggccatc gctccccaaa cgctcgccat ttcgggaaca
     7741 ttaggcattg gggtaccaac ggaagcactc ttcgaaaact ggagaacatc atagagctct
     7801 ggcccagcat ttgcttcaat gaaggaaaag acgtccgctc tagccgttcc tcttctctct
     7861 cccaagaaca taccatactg gccttcgaag ctacctatgt agtttaccac aaactcggtc
     7921 gcttcaagct tgttcttgga cttggcgtta atcatgaatc cctgaactcc aacgaacggc
     7981 ctcggagtgg ttccatcgtc aaatgtgatt tcgctaaacg gaagaatcgc gtaatcaatt
     8041 cctgcgtcac ggtagcctgg agtggcccaa ataccgttca ctataaaagc ggcgagccca
     8101 tctttgaaaa gcgagtccat cagattgtag ttcggtcctg cgggaatgag acccttgtca
     8161 aaccatgatt ttatcaactt tccaccggca attgcgcctt cgttattcag tcctatatcg
     8221 gttacgtcga ggccatctgc cgtttccttg aaaatgtagc ctcctctgcc cagtatgaaa
     8281 ggagcagagt gatagaagtc gccagcggta taaatcagac ctacgacttc atcgtctgat
     8341 gcctcgagcg ccaactcctc tagctcagat attgtcttcg gttcttcaaa taccaggtcc
     8401 ttgttgtaaa ttatcgcgat cgcttcaatg gtgtagggaa caccgtatag cttacccccg
     8461 tagctgaagg catcgagagc aacttcgtaa tattgatccg agtctggaag gaatgggatc
     8521 ggttcaataa ggccgttgag agcgagctct ccaacccagt cgtgggctcc aacgattatg
     8581 tctggtccct cgcctgcggg agcagcggtt aggaacttag actttatatc cccgaagttc
     8641 acttgttgaa tttccacctg aataccgtag tcgctttcga aatcagccgc cagtttctca
     8701 agtgccggga tctggttttc tgaggaccaa ataacaagct gtgcccctgc aaaagacaag
     8761 agcacgaata gcataagaag aacagctgca agttttttca tcttcaaaac cctcctttga
     8821 tagaatggtg tactgcctga ataaattgta tcagaaagtt ccgtttattt taagatgtgt
     8881 tactgtgtct actacagata gaagacgcct tcattctccg tgaaggcgtc cattcgttcc
     8941 tggtcttccc taatcgatga tgatgatgct tctttccagg taaaaattgt taatactacg
     9001 aattaaactg gatatactgt tattccctct ttgaaattct tagcgacatc gaaatcgact
     9061 ctcaaagagt cattgtatct ttgagtcaaa aacctcttcc cgacttcccc aagcagcaaa
     9121 tagataagca gatcttcatc gctattggcc aagagaccta gttcattcct gcccttctcg
     9181 acttccggct ctagagtgtc gcccggtcta tgagaaatcg atttctcgtc acccatcacc
     9241 cgagtcttca acgtgctttc gatctccgcg ggcggcgaac cgtagaagcc tttgatgtaa
     9301 ctttcaactt cctttgttat agtcttgtat ctttcattgt tcatgacatt aagaacggcc
     9361 tgaacaccta ctatttgaga ggttggtgta accaggggcg gataacccaa atcggcgcgc
     9421 actctgggca cttcttcgag tacctcctcg aatctgtcac tcatattttg tgccttaagc
     9481 tggttgatca gattggagta catgcccccg ggtatttgat tttcaagaac cgatgtgttt
     9541 atagtgaaac cggcatcggt ttcactatgg ttcttacgaa cgcgaacaaa ataatcgttt
     9601 atctctttta gcagtacgta atccggctta tctgcaagtc ccaagtcgtg caacgccgtc
     9661 cagacacttt ccaccccggg ctgtgaagtt ccaaagccca ttgaagaggt tgccgtatcg
     9721 agaatgtctg cgccggcttc ggcgctcttc aaataggtca taccggcaat gccagtagtg
     9781 aagtgagaat gaacttccag cggaatggag tgccttttct tgatctcgct tacaagagtc
     9841 catgcggttc cgggtttcaa gaggccagcc atgtctttta tacagaggga gtctgcgcca
     9901 agttcttccg ctttgtcgga aatctctacg aagtattcga cgttatgaac tgggcttgtt
     9961 gtataagaca tggctatctg aaggtgcatc ccgctttcct tgacggcctt tgccgacttc
    10021 tccaggtttc ggaagtcatt aagggcatcg aagattctca ctatatcgat tccgtgtttt
    10081 gccatagttc tcacaaacaa ttccacaaca tcatcggcat agtttctgta acccaggagg
    10141 ttttggcccc tcaatagcat ctggagcttc gtttccttga aactttcctt caatatatca
    10201 agtctttccc atggatcctc gtttaagtat cggacacatg agtcgaaggt agccccgccc
    10261 cacacctcaa ctgcgtggta gccaacccga tcaacaagag gagctatctc cgccaattcg
    10321 ccggtagtaa ccctcgtagc aagaagtgac tggtgtgcgt ctctcatcgt agtatcaacg
    10381 aaacagactt ttcgcaattt catgcctcct ctctgtgtac tatacttatg atagaggagt
    10441 gatgttatgc gatcaaataa aagagattac gaattcgaca taattgttat aggcgccggc
    10501 catgcaggta ttgaagcggc tcttgccgga gccagaatgg gcatgaaaac tcttattcta
    10561 aatatcaacc ttgatacggt tggatggacg ccctgcaatc ccgcaatggg tggtcctgca
    10621 aaggggatag ttgtaagaga gatagatgtt ctgggaggcg agatagcaaa gtcaaccgat
    10681 caggcatcac tcaatataag aattctgaac accagtaaag gtgcagcggt tagggcgttg
    10741 agagctcagg ttgacaagat taggtacagc cagacaatga agagaagcat ggaaatgcag
    10801 gagaatctct tcatacagag cggaatagta aaggctatag aaactgaaaa aggaagcgta
    10861 aagggtgtca tcaccaattt tggcctcaga tactcggctc gctcagtaat tgtggcttcc
    10921 ggcacttttc ttggtggaaa gatatttatc ggacctcatg aatttccagc cggcagactt
    10981 ggagagtttc cgtctacaga gttgaccggt tcactgcggt cgatcggttt tgaagtaagg
    11041 cgtttcaaaa ccggcacacc ggcaagaatt gccggaaggt ccatcgactt tgccgagatg
    11101 gagcgtcagg gaacctccga aactccgctt tgtttctcac acttctccga accttacgtg
    11161 ctggaagacg atcacccttg ctggcttacg agaacgaaca aagagaccca tgacattata
    11221 aagagagatc tgcatttttc tccgttgtat ggagacttaa aactcatcga ttcgagaggt
    11281 ccacgttact gtccatcaat cgaggacaag gtaatcaagt tttcctccaa agacagccat
    11341 caacttttcg tcgaaccgga gagcgcttac agcaacgagt actatttgaa tggtctttca
    11401 actagcttac cttacgagac ccagctcgaa atgataagaa caattccagg actaggcaga
    11461 gcagaaatag tacgaccggc ttatgcaatt gaatacgact acattaaccc cactcaactt
    11521 tactcaaccc tggagagcaa agtagtcgag aatctttatt ttgccggcca ggttaacgga
    11581 acgagtggtt acgaagaggc ggccggtcag ggactcatcg ctggaataaa tgcctcggcg
    11641 aaactgttgg gagaaaagca gctagtgctc agccgatccg aggcttatat tggagtgatg
    11701 attgatgacc ttgtgacgaa aggtgtcgat gaaccttata gattgctgac ttctagggca
    11761 gagtatagat tacttctcag gcacgacaac gctcatatta ggcttaccga gtacggttat
    11821 agatatgggc tggtgcctgc ctggtttaag gaaagagtag acagtctgac cagagaaata
    11881 aagactcagt tgataaggct ccagaagatc aatgtcagac cttcggaggg cttgaacgag
    11941 ctgctcatca gtaagggcag ttcaaaaatc gatttgcccg tgaagttttc ttcgctgttg
    12001 aagagggccg gcgttggtta cagggatatc tccatgtttg atccagaacc gatattggat
    12061 gacgatgtta ttggacaagt tgaaatagaa gtcaagtaca gcggctacat cgaccgactt
    12121 aaggcagaag ttaagcgtct cgaagagatc gaggaggatt taattccctc gactatggac
    12181 tatgatgccg tatcaaattt gtcaacggaa gcaagagaaa aactgaagaa gaccaaacct
    12241 gcatctgttg gccaagctat gagaataccc ggcctcacac cctccgacat aatgaatctt
    12301 tcatttcatc tcaaacatga gggggaaaaa tgagcaccga gcgatcgccg gtgcttatcc
    12361 cgatagcaag gtctgtaacg ttttttccag atctctatgt tcaatagact ttccgtcttt
    12421 cagaaggcaa aatgcggcct ttattacaag gactcgggga tacttcacag tcatttcctg
    12481 aaccggatct gtagatatcc gaagacccac agaactgctg tttagcatta aaccatctcc
    12541 tgaacctctt gacacaagcc atgaggaata ttataatcaa atctgtgtct gggctcgtag
    12601 ctcagtttgg ttagagcttc cggctcataa ccgggcggtc ggtggttcga ctccacccga
    12661 gcccaccaga tatggcggct ttacgccgcc tctttcttta ttaattctag cagttttgct
    12721 ggtattcttc aagagagttt gcctatgttt aacatttagg acatgaaacg taaagaaagt
    12781 ttagtcagca tttatgtaaa atataggttt ttgattgtca gccgatttca ctcagtgaaa
    12841 agaccaatct tcctcctacg aatgtcatat ccgaaaacct gtcttcattg gtcttcaata
    12901 agtcagcatc gataacaaag agatctgccg ggtaacccgg ttcaagtgag cagcctctgc
    12961 aactattggc cagaagatgc ccgttcgaag tgtacatttc tatgctttcc cttctcgaaa
    13021 agtatctgtc tgcagagttc agatttctaa tcactgacgt actctccacg ggagaatccg
    13081 ttgacaatgc aagaaggaca cccatatctc tcatctttcc aaatggatat gcgttctcca
    13141 agcgagaagg gccgaatctt tccggagcta tcaatttgtc ggaatcaacg aagactggct
    13201 gaatagatgc agtgagcttc tcttctacca tccttcggat ttgctcttca gaggcgatct
    13261 gaacgtgaat caatctgtgc ctaagcgttt cactcttcga tccttcgaag gccttcagtg
    13321 cttcttcgag ggcacgatcg ccgattacgt gaacgcaaac ctgaatccct tccccatctg
    13381 cccttttcac aatcggtcta atctctttcg cgctcttata gagcacgcct ctttccccgg
    13441 gagaatcata gtacggttca atcatagccg ccgttctcgc tccaagcgaa ccgtcgagat
    13501 agagttttgc cgcatttaac tgaaagaatt cgttttcgaa ctgtcggccg agtagtatca
    13561 tttcgaggtc ttcttctcgc gatacacaaa atttctcgta aactcttaga tcgacaatgc
    13621 ctgcgagtct ttgcatcagg agttcgtatt ctatcccgtg gtagtcatcg ctgtgcactg
    13681 aggtcacgcc gtatttcaag aaggcctttg ttcctgcttc gattgcagcc tctacttctc
    13741 cagaagttag caatacgttt ctttcgactt catcaagtgc cctctcttta aggatcccga
    13801 gatcaatgtc cgttccgtcc agtccatgaa gtcgttcgag actaaagagc tttatgaaag
    13861 ccgaattagc agaggcgata tgaccgcacc ttcttgtaat tattactggt ctagatatga
    13921 ttgaatcaag tatctttctt actggcatga agccaagaac ctcctgatcc caaccccggg
    13981 ctcgaacgat atcgcgttca acattcagag ccgtttcaag cttttctacc gagtcacagt
    14041 tttcgagatc gacttcaagc tcctttcttc cagttccgat tagatgagcg tgagagtcca
    14101 caaaaccagg gtagacgaat ttaccgctta catctatttc tatcgcatct tttgccggta
    14161 cttcagaaat tatgccttcg ctaatgtaga gatctcgggt gacatatctt ccgtcgggta
    14221 ccgagtagac tctgcctccc ttaagtgaat atgccattat agttcggctc ccaagctccc
    14281 cgctatttgt ccagcaaggg ccgcattgtt tatcaaaaga gagatgttcg cgtcgagagt
    14341 cttgccgttt gataactcag cgatagtgct taggagaaac ggtgtcagag ccttaccgga
    14401 taccccccta gcttcggcag ttgacagagc agtctctatt aacctggaaa ggtcgtcgta
    14461 atccatcgaa tcggtttctg ggatagggtt cgctacaagc accgcaccat cgatccccag
    14521 tctctctttc tcttttagaa tcagcagagc ctcttccggt gaatttatcg tgaggttgac
    14581 tctataggga cttctggagc aatggaatag tgggaatgag tccgttttgt aaccgaccag
    14641 agtgacacca aaagtctcga gaaactcaag agtcttaggg acatcgagga tgctctttac
    14701 accagccgat acaacgatca tactggttcg ggaaagctcg ataatgtctt gagagacatc
    14761 ccagtcttta ccacgatgaa ctccaccaat gcctccagta gcgaagaccg aaataccatc
    14821 tctctttgcg attgccatcg tggcacttac cgtggtcgct gcatctctct tcatcgctgt
    14881 ggcgtaggga atttcagccg ttccgacctt caaaacattc ggagccgttc ccagcctcat
    14941 tatctgctct tcccgaagac caacgattat ttctccgttg agaattccaa tagtcttcgg
    15001 ttcaccgcca ttttttctga ctgccctctc catgtttaga gcagtctcga aattgacacc
    15061 ccttggaaga ccgtgggcta tcacggttga ttcgagagca acaacattgt tcaacttcat
    15121 tcacctctca gacatttagg catcggaagg ccctttgttt tcaatctcat tacttcaaga
    15181 tccctggact gggcttgtca attgaagctg acttccaaaa aagctttcac gcgccctgtt
    15241 tgaatcaatt ttatcagaac gcgattgaaa aggatggagc gtaatgctat aattctgacg
    15301 ttgtgcccct atagttcaac ggatagaacg gtggattcct aatccgcaga tgaaggttcg
    15361 attccttctg ggggtaccag aagcgaggat atcctcgcct tttttattgg cgattagcat
    15421 acaggaaagt ggcaccgaaa aggttaccta gggtacgtta attagtcgta gatgctgtct
    15481 tttgccgata tttaggtttg agaagaattc gagatggtca tagaattcat ttgaggatca
    15541 ttaaactgag gaggtattct tgtggccgaa actagtttgt ttcaaaatgc gttaaagcaa
    15601 ttcaataagg cagccgatgt gatggatctt agccaaagta tgaggcaggt cctgtccttt
    15661 ccgaagagag agcttacggt aaactttcct gtcagaatgg ataacggtga aatcagggtt
    15721 ttcacagggc atagagttca gcataacgtc gcccggggtc cggcaaaggg aggaatcagg
    15781 tatcatcaga acgttaccct tgatgaagtt aaggctcttg ctttttggat gacatggaag
    15841 tgcgcggttg taggcattcc ttatggggga ggaaagggtg gggtctgtgt aaacccacag
    15901 gaactttcta taaacgagat tgagagactc tccaggagat acttctcaga gattcagata
    15961 atcatcggtg aaggagaaga catcccagca cccgatgtaa atacaaatgc gcagataatg
    16021 gcatggtaca tggatacgta ctccatgaac atcggccact ccgtactggg aatcgttacg
    16081 ggaaaaccac tggaaatcgg agggtcaaag ggaagaacag aggccacagg ccgcggagta
    16141 agggttgttg ctgaagaggc gcttagatac aaagacatgg atccaaagaa ggcaaaagtg
    16201 gccgttcagg gcttcgggaa cgtcggttcc tacgcggcaa aactgattgc tgaagagatg
    16261 ggttctagag tggttggctt gagcgacgtt tcaggcggcc tatacaatcc cgacggattc
    16321 gacatcgatg atctgattgt ctacagagat cagaacaacg gagtaatcga aggttatccc
    16381 aaggggcaga agatcagcaa tgaagatctt ctcagtctcg acgtggatgt ccttgtccct
    16441 gctgctctcg agaacgcgat tactgaaaaa aacgcgagga acgtcagggc gaagatagtt
    16501 gttgagggtg caaatggtcc tatgacccct gaagccgaag atatgattct tgcaaacaac
    16561 atctttgttg ttccggactt ccttgcaaac gcaggtggag taacggtatc atacttcgaa
    16621 tgggtacagg gacttcagca ttacttctgg gatgtagaag atgttaggag agcgttgcat
    16681 aagataatga aagaggcttt tggctctgta atatcgacaa tgaacaaata cagtatcgat
    16741 atgagaactg ccgcatacgt tgatgcaata gagaaagtcg ctcttgcgac gaagcttaga
    16801 ggtatctatc cctgatagtc tgaacggaga gcaaattaat tagtcgagcg gaagaacttt
    16861 cttccgctct tttgttgtga cggacattca tagcctttcg gaaagataga aattgtcatc
    16921 aactctgtaa gccgttaaga agccaccgta aacacaaccc gtgtcgatac cgatcttgcc
    16981 ctcttgaatg agtggaccac ttattaatgg cgtgtggcca tagacaaccg ttcgaccctt
    17041 aatcgggtag ctgctctcta gaaagggttc tctaatccaa aggagatctt ttggatcttg
    17101 cttcttcaga ggaattcccg gtctgactcc tgcatgaaca aagatgaagc ttttctcttc
    17161 atagtacaga actgtgttgt tgagaaactc gatgtgatat tcgggaatgg atcttatggt
    17221 tccgtaactt cttatcgttg tctccattcc gttatatgcc catattgtgg aaccctgaac
    17281 gccattcaag taatctagga acatctgctc gtggttgccg cgaatgaagt ggcactcgta
    17341 attcgctgaa agcgcaatga gaaagtcgat aactcctctg ggattaggac cgcggtccac
    17401 ataatctcct atgaaaacaa gcgagtcgtc atccttgaga tcaagggcac taatgagtct
    17461 tttcaaaggc gaaaggcaac cgtgaatatc ccctattgca taggtcaacc caaaatgccc
    17521 ctcatcagca actgccacaa gtatgatata agcactcccg caacgggact gataatccat
    17581 tcagtcttct tttctttcga aaacacagcc cggaaagcaa gccccgcggc tacggatatt
    17641 atcacccagg tccattgatt tgcgagaata aagatgaata tagacattct ctcggcaatt
    17701 ccaattgaat cagtcgcttc ttcctttata atccttcccc gaagaaaata ggttactcct
    17761 accgttgtca gaagcattcc catcagatac caggaaaact ctacggtaac aaaggattca
    17821 atcaaaaaac ttcttccaag caatgtcaag aaaagcaaaa ggaagaatgc aattatctct
    17881 atcagttcgt tggaaagtct ctttcgtccc atatcaagag tgaccgtcaa acctatggac
    17941 agtattagaa taagaattcc aagcggattc gataaaaaga catcgaaagt gaaagcaaga
    18001 aagacaagaa caacccatac aaaatgcatc cagaacttct tgtcatccat ttttgccagt
    18061 ttcttgttat tcacaaaagc atgatcaact acataatagg ccactaaaag atgtaatggc
    18121 atgtctcaac ctccgatctt ctctctgctg ttccatggta gcacgggaac aagaaaagtt
    18181 gcaacaatca gtgcggccaa aacaccaacc ccgagactga taccgaacgc gctcaaaagc
    18241 ttcccctttg caaaaacaaa tgaaaagaag gtgagcatcg ttgtggccga agacatggtt
    18301 accgatttaa gggtttttga tagctcggaa tttcctttcc tcttagaact atgcataacg
    18361 tacacaagac cgtcgacccc aatacctatt atcaaaggag cggaaattac cgtgacaaag
    18421 gttgtgtcaa cgcccagcag ttccatcaaa ccaaacatgg acatcaaaat acctatcata
    18481 agaaggagta ctttgaaccc gtcgattaag gactttctct ccgccgccac aactacaaag
    18541 atcgctagaa aagcgacaag acatattgaa agtgaaactc gccttacaag atccatcaga
    18601 taatagaaga cctttggata tcccccaaag gtctccttaa gttccggact tagactgtcg
    18661 tatattattt ttataacatt gttcctatat atatacgatg acggctcgga gttcaacaga
    18721 aagtaagtct ttccatcgcc acctaagtaa agcaactgat ctcttagact ctccgggatt
    18781 acttcagtaa caagacttat tagatcatcg gagctctcca cagtcccagc cctatcgagc
    18841 aactcgacga taaaatcata gtagtttgct ttcttgaata ccgcttcaac aagcggtgag
    18901 tctcgcagtg cgtcgatctc tctcactacc tgctccatct gactcatgta atctcccttt
    18961 cctccttcaa caatcgaaag aatggagaat gagcttttga agttaggatt ctgatcgatc
    19021 tcctcgtgcg cctttctcaa cgaatcaaga tcgggaagta tgaaaggaac cgtgtttatt
    19081 atgctgccgc cgaatgtcct ttcgatgagg ttcggaatcg ccacttgctc agagtcgggt
    19141 gacatcaaac caggaggcgt gtaggaaaag ctcctgatgt tattcagtcc cagataactc
    19201 agtatgagga tcaacgcaaa aacaactcct cccgagataa ctccgaacct tatgattact
    19261 ctggaaatct tctgaaagaa tcgatcaagg agatgtatgt ttgcaacctt tgttgaatta
    19321 atgagggtct tgattgataa ggccggaaga aagacaatca ttacaacaaa gaagactacg
    19381 atcccaatcg atgaaagaat cccgagttct cttatggcgg gcgagtcgat tgcggcaaga
    19441 attatgaata ccagcgcagt cgttatcatg ctcgccagaa gcggtctggt aagtgcacca
    19501 tatgcatctc tcagcagagc gaccgcgttc ttctgctttg acgctccatc ggaaagcctt
    19561 gtgatcatat gaattccgta gtctattccc aggccaaggg taatcgcagt gacaaaagta
    19621 gttattatgt tgagggatcc gaaaacaaca taggaaatcc ctagagtgat aaacatggaa
    19681 attgccagag aaaagaagac atagagagta atcttgagac ttgagtaggc aaaaaagaag
    19741 agaagagata ttcccacaag agagataatc gtggtgagca tgaagtcatc gttaacttgc
    19801 ttgtttgaat cgtacgtccc gaagtgatcg cctgccatgc caaaagacaa accatatgaa
    19861 ctgcatatct ctcctaccca ccgtctcaga aggggtatga ctctgtttac ataagacacg
    19921 tccgaagaag gaccatcaag tcttataccc attaccagga ccattggatc gttcgttggg
    19981 atcgtttccg gcaaccttac aaaattccag aattcgtcgg attgcgggga attccccagg
    20041 agccctctga gctcctcaaa ttcaacaagc atctgaccta tgctgcggac agctgaaaaa
    20101 tcgtaattga cgaggctttg aaccgttcta gagaggtcct ggtctcggct tctcattgaa
    20161 gagagttcag cgggatctac cataagaagc ccgagtggcc ccaaatctga cataacatcg
    20221 aatcttacga aagattcgat attcttcatt tcattggctg tttcataaat ctgcctgcct
    20281 gcagaaatca gattctctat gcccttttca gttttatcgt cagtttggag tacgatatat
    20341 gtaacgtctc cagaagtatc cccgctaatg aaacgattgt attgtttcac aacaggattg
    20401 tctttgggta gtatcttgag tagatctgaa tcgatattca gcttcgtgga aagatatgca
    20461 ccgaaggcgc caagtataac gaaaaaaact atgacggcaa cacggtgtct aatgacaaat
    20521 tcactcaatc tcaacaacta accatcactc accatcgaat attcttgtcc ttagaaaagc
    20581 atctcaagta taccaacttt gaaatgaaga aacatctttt ttgctaatcg cttcagtctt
    20641 ttatcagaaa ataccttacc ggaagatatg gtattgagta gcgctccaca gtttcaatga
    20701 aggactggtt gatcacgccg ccctctctta aaacgacaac aggaccactt ccggcaaaga
    20761 cgtctgagac aaaactcccg ggacttatag agtgcataaa atgtactctg taaaacctga
    20821 atccttcttt caggaatgaa ttgtagtctg actccatgac aagcgtgtaa ccaggcagat
    20881 aagtaagtcg gaaagctccg taatcgatgg actccattct agttaccgtg ctaccgaaaa
    20941 actctactgt ctcgtgttct cttataccgt tcttgcccgt ttcaatgtag aagagagtct
    21001 gcaacctaga gaagacttcc ttgtaggggc aggtaaacgt tctaatgcct ttttcagccg
    21061 acatgatcca gggtccgaca ccttctctct cgagttgctg gagaacagag ctgaaatcct
    21121 ttgtctcttc gagatgattt ttgaatcgct caagcaaagg tataaagctg tttctgagaa
    21181 agaagtcggg agtcgctctc cggctcccga cattctgaat tgtttcaaaa ttcatgtata
    21241 caattacgag aagcccacaa atcagtgcga tgagcgaaat cagtattgaa acacttgcta
    21301 tcttcaattt acaaccttaa ctcccagttt ctcgagaata gtctcgatag ctttcgaaag
    21361 ttgaagatcg tgttccacat ctacagtagc cgttgaaagc gtcagctctc tcccgtcctc
    21421 actgggagcc tgctcaccgg cttcaacaat tatgtctggt tctattccct gaaggtgaat
    21481 atcacttcca ttcggagtaa aataatgcgc ggttggaagc catatttctc ctccgttcga
    21541 gagattaaat actgtctgaa ctgctgcctt cccgaaagtt gtcgttccta taaccgttgc
    21601 cagtccgtga tccttaagtg cccccgtgag tatctctgaa gcagaagcac tgccatcgtt
    21661 tacaagcaca acaatcggct tgtctgtgaa gtaggaatta tatcttgatc cccaggaatt
    21721 gtaaacctct tcctgaccgt ctctatatct tatcgttatt actctcttac cctttggaag
    21781 catcagtgat gatatgttca aaacgctctg taatagcccg ccaggattgt tacgaaggtc
    21841 gataatgaac gccccactct ctctgctcaa attcagagca tcctggaatt catcataagt
    21901 tggcgcgttg aaaccggtta tttgaatgta agaaatttcg gtatcttcaa cagtaagcaa
    21961 ttccgtcttg acactcttaa tctctatgaa ggctcttgtg atctctacaa aaaaaggatt
    22021 ctctacggat tctctataga cctcaagaac tacctgggaa tcggggtctc ctctcagcat
    22081 attcactgcg ccgtaataac caacttccga tactggcact ccatcaatcg tgagaatcaa
    22141 atcgcctgct ctaaggccta ctctttcgga agggcttccg gccatcgggg ccaccacttc
    22201 tagacaatcg aactcaatgt tgtactgcac agtagagcca atcccaccgt acttggaggt
    22261 ggtatccagt tcgttttcct tagtttgaac cgggtcaaag taccatgcaa agggatcgtc
    22321 tagtccacgc atcgttcctg tcaaagtctc atttaaaacc gcgtcataat ctaccttttc
    22381 aatgtcgtaa tagttgcgat cgatgtacgt aagaatctcg aaaacggggc tgaacttctc
    22441 gaacatttca tcgtctgtca tcgatgaaag tgcgcccaga aagaaaactg caaccaggac
    22501 tgagacagtc aacaatatct ttaggactct attcttttcc atttaacttc ctcccctcta
    22561 cttttatgcc aaacaccagg tatgtagtat gaccgaccat cctatcttca ggtctgaatc
    22621 tttcggggtt tgccttatag cttctggcaa gtatctccca cacctggata tcaatgattc
    22681 ccagtgttcc tagaatcgtt acagtctcct gaacctgatt agctgtcggg cagagaatgc
    22741 agatcctgcc acttttctta agaatggata ctgcgctctc aagggaagcg gagggttcgg
    22801 gaacatccag aaagaaggcg tcggctttct gactttcttt gcattccgca acatccccag
    22861 atacgaacgt cacccgttct atgtggccaa gatggcgaac gttcttctgc gccttttcca
    22921 agaaatcctg tctcttttcg tatgatacca ccttgcccga ctcacatacc gctcgagaaa
    22981 aagcagaagt cattacaccc gtccccgttc cggcttcata aacaacatcg cccggcttta
    23041 tgtcgaggta taacaagata aacgcgacat cctttggata cactatctgt gttgatctct
    23101 tcaaagagaa gatgccgtca atcattgatg gttttatcag gaaggcatcc cgaagcccca
    23161 atctaatctt ctctccgaat tctcttccca caagagaatc cattatcagg tttccataat
    23221 gtgtctttag gacactgcct ctcttcacct cagagaagta tttcgcctct tcgtgttcaa
    23281 gcattatcag aatgctgtca ccgtattgaa tcatctaaac tcctctaagt ttaattatgt
    23341 tctcgatcag agtgtcaagc tcttcgatct gaccagattt actggcctca attacgcact
    23401 tcctcgtata acctttgact atcatttctg atactttcga gagtgcagag ttgactgccg
    23461 acacctgcgt cagaatatca ccgcagactc tgtcattttc aaccattttc tgcagccctc
    23521 taatctggcc ctcgattctt ttcagtctgt tcaggacagc tttccttgtc tcattgtcta
    23581 caccttgcat ggcttcacct cataaggaac cgaatacact gtcgccaatg aattccttga
    23641 taatggaggt tctcggaata tcctcgaggt tcatcattgg aagcaggtaa tcaatgaacc
    23701 tgagaagcct cttagcttcg atatattcag gtacggaatc atcaatctga acaagtagag
    23761 gctctgcaaa tccctttaac gctgccagtt catacactat ggcagaattg aacatgacat
    23821 ctgcctcttc ttgaaaggga aagatatact tatcctctcc tcttcggacg cttggccaca
    23881 tgcgcatcgt gtcaagcgcc gagtgacctc tcgatctgtt gtcacgcact attctgcgca
    23941 gcaatctgac gtccgtagtt ggtattctgt tcagactgtc gagattcatt tgcgtcaggg
    24001 cgctgacata tatcttgaat ttcctgtgtc ttggaacgga gctgcttagc aattcattaa
    24061 gaccatgaat tccctctatg atcaccggtt gaccctcgga gattctgatg ggtttgtcat
    24121 ggaagccgct cttgccaagc atgaaatcaa atttgggaag caagacctct ttgccatcca
    24181 agagttgcga gatctgttcg ttgaacagat caatgttaag agcatgtatc gattcgaaat
    24241 cgtaattgcc gttctcgtca agcggagttt tttctctgtc gacgaagtaa tcgtcaatcg
    24301 atatctgcac gggattcata ccgagcacct tgagatgaag catcagcctc tttgagaagg
    24361 ttgtctttcc ggaacttgaa ggacctgcta tgagaatcag actgggttcc ttatgtttgg
    24421 caatctggtc tgcgatagca ccgatcttct tttcatggag ggattccgta atattcacta
    24481 tctctcgaat ctctcttggg cccttgctga ttatttcgtt tatctctcca atagttttca
    24541 ctccgaggat ctcgccccat ctttcatgtt cgagaaagat ggtagagatc tttggctgat
    24601 cgacgaactt cggtaacctg tcaggctgtt ttgtgtcggg gagattcagt ataaaataat
    24661 ctccataggg tattagggaa aaattcagta tagctccggt cgagtaaggc atataaccgt
    24721 agaagtagtt tatgaatccc ttacatctgt agatattgac ggtcgatttc tttctgtatt
    24781 tgaagagcag agccttgtct ctctgtccat cttcactgaa aagccgtatt gcctcgaatt
    24841 tcctcagcgt ggccttttcg aaaacgatat cctctttgac taactctctc attctctctt
    24901 cgatttggag gagattctcg ggagtgcttt ctattccctc aatattgcag actagtcccg
    24961 ttcctctgga gtggtgtaca ttcaactgtg ccgaaccgaa tagctccctg attgcaatga
    25021 agagtatgaa cagagctccc ctggtataga tccttattcc atcgagagaa aagagatcaa
    25081 tgaattccac ttcgctttcg ccgtttacat ttctacaaag ttctattaaa gagtttccag
    25141 atttagcagc cattatggga gccttgtgga gttcgccgca cattttcgaa agttcaaaga
    25201 aggaagtctc tttcgtcaca tggatcttct tgcccaattc ctttacaaaa atctcgtggt
    25261 cagccataga atcactccaa atcattgaca ccacaaatcg tcgatgttag aatctatttg
    25321 ctgtggggcg tagccaagtt ggtaaggcat cagattttgg ctctgacatc cgtaggttcg
    25381 aatcctgccg ccccagccag aaaagggtgg cgtaagccac ccttcgttct gctagttaac
    25441 agtctcagta gctccttcac tatcgaaatt cacgtctaaa tcaacgtctc cgatagaaga
    25501 atgctcatca ttacccaaag gaacttccag ttcgcctgta cctatctcag tgtcgataag
    25561 cgtttcagtc gaggtttcag tagaagtagc gtcagctgtt ggagttgcat cgacgttatt
    25621 ctgagcattg agttcggaaa tctggaattt tattgtttcg atgagcgatc ttatagtttc
    25681 gtcttcggga tagagttcgg caagtttctc aagatagtgc ttttcactga gaaggtatat
    25741 ctgcgttagc tgctcgttct gctccatgtt cgccatcttt tcatatatat ccacaaggag
    25801 gtacagagcg ctcatcttga gatccgtagc tgcttcggtg gagttgataa ccttctgatt
    25861 aagatcgctt tcaattccct gaattggaga atccaggaga agtgatcttg cctgagcgcc
    25921 gaagtactgt ccataatatt caagatagct gtcgagtagc tcggggcttt cgagaagagg
    25981 ccttatgttc gtgttataag aagtctcgtt gtacatgtat accacttcgg gattatcacc
    26041 gtcgatctgg tacatgtagt taatcactct cgctgaattt ggcatcacag aataaaggta
    26101 tcgcaccgaa ttctcgaatc gcaagttctt gctgtcaagc aaactacgtt tctccgcgag
    26161 agcctcttca atttcttcag gagtctctac gttgaattca ctcatgtact ctttggccct
    26221 tttcgcgtcg gtaagaacac ttctggcctc cgtatctgta gatgggatcg tctcgagccg
    26281 gctgtcaatt tcatcgatcg tcagcgaagt ggccgtagaa ggcagagtca acagtaaatc
    26341 ctgaagatct tcaagatcaa atatctggtt tctaagcttc gttatctcgt tttcggcgag
    26401 ttcggtgtag aaaacaacgg gaagatacga gtctccaatc aggagattgc cggattcgtc
    26461 gaagtagtct ttctcgagct cctcaagaag cgcacttgac ttttctgggt cggaaattgc
    26521 ctcgtcgtat ctggcgtaca tctgcaaatc cggatcgttg attgtatatg agaggttgtt
    26581 ctgttgacga tagtcggcta tccatttatt gaactcctca ttttcaaagc ggcctagctc
    26641 agtctgatag gcagaagtgc tggcaaactc ctcgaaagtg tcgaaagttg ttttgctgtt
    26701 aacccgtatc aagtggtagc cgaactgcga ttccacaggg cctattacaa gaccgggcgt
    26761 agcatcgaac gaggcatctt caaactcctg caccatctga ccccttttga attcaccaag
    26821 ggctccaccg tttatcgcag tgccggtatc gatagagaac tgagtcgcgg catcggtgaa
    26881 cgaaatctca ccgctgtcta tcatcgactt aatctcaacc gcactggctt catccgagac
    26941 gagaatgtgg tttgcatcaa ctttctcata gttgatcttc agatcttcca tgttttcgtt
    27001 gacgtagttc tcaaatctct cttccatgtc gggaatagct ttctgcgtga cattagttgc
    27061 aagcatttgt gttaccacgt agtctcttac cagttcagag aaagcatcca ccgaaccgta
    27121 ctgataaata attgcggatt ttgtttcctc gtctgccacg tattcatcga cgattctatt
    27181 ggtttccgca tcgacctgtt ccgcagttgg aaggacaccg ttctctgtcg cgtagagaag
    27241 aagtgtcttc tgatctatca atgacttgaa caaatcatat ctgattccca tttcactggg
    27301 atagttttca gtttcaaagt atggatcgag gttgtaaccc tgactccgaa gattcgtaag
    27361 tgtgtcgcgc acagacgttt cgtattctga ataagtgatc cagtaatctg aatttgagag
    27421 aggggtaccg tcttttgtaa gccctccgac agtttcggta aaactgatct ccgtaccggt
    27481 gccctgctca ttgccctggg agctccctcc aagatactgg gcaaccgacc accagactat
    27541 tccaactcca aagagtatgg ccacggccca cacaatcggc cgctgaatgg atttcatgaa
    27601 attagaattt cctctcgatt ttctgtttgg catttagttt tcaactccct ccaagtacct
    27661 aatactctac aaccaatagg gggaggtttt gcctcccccg gcttcacgaa ttataccata
    27721 gtaaatatgc tcttgtaatc ggcacgatat caagaaagat ggccgatgta actaaccaac
    27781 ttgctttgcg taaaccctga aatccaggta tggaaatatc gagtcaatcc attcaagggt
    27841 cttcaacctg tgatcatcga tcgagtcact ctcgatctct ctgtaaagcg tcagcaacct
    27901 gtcgatgtga gtctttgttc tgaagactgc atattctaca gttgtgttgg tagttattat
    27961 gaaagcccaa tcgcttgact gagcaaggag aagctctctc gccatctgat ttagggctct
    28021 aattttgaga ggatcttcag ttctctcata cttccttgca agctctatca ttctcctaga
    28081 accctcgtcg agatgcctgt atatccagtc attcctaccg ttcagccaaa cttcattgta
    28141 accgttcgct ccccatgtcg atgtatttgg ggttacaacc tgcgcctgtt ccatggactc
    28201 aatcacttcg ggtggtgtcg atggaacaac ggtttcgtct ccatttagtt ctctgaatag
    28261 ctcttcaata aagaggggcc cttcgtacca ccagtgtccg aaaagctcgg catcgaacgg
    28321 cgcaacgatg acgggttctt ctccatcgaa ttgcttggaa agccttctca cctgttctct
    28381 ctttctattt gcaaaatctc ttgcatgctc gatcgttgcc tttcttgcat catcgatgtc
    28441 gtagtagctc ttatcgttca gagctacatc cttccccgtt atcttgtaat acttgatgcc
    28501 agtattgcac cgctcaccac tcggatctat gtagggtctt atatagtcct cttcacgatc
    28561 gaatccaacg tccctgtaaa attctctgta tctggcatct ccgggataac ctacctcagc
    28621 gctccaaact tgctggctag attcggggtc tcttgcaaat acaaagacac tctccggagt
    28681 aaccactggt ctgtacactc cgtatcttgg agcgggatcc ccgtaccaga aactgtgcga
    28741 atcgacgaag aagaacgaga gaccatgttc gcgcaggtag gagtcgaagc caggcacgta
    28801 tccacactcc gcaagccaga ttcctctcgg atcctttccc acatgtctct tgtaagactg
    28861 caccccgatt tcaacttgcg cattgattgt ctccgggaag tttgtcataa ggggaagaaa
    28921 accatgagta gcgttgcacg ttacgatttc cagctctccg ctttcctgga gctgggcaaa
    28981 ctctccaaca aggtctctgt tgcactcttc ataaacgctt tttatctcct ggagctcttt
    29041 caaatagtgt aaggctagtc tattcgcagc tggatgctcc gagaccgttc tcgaaatctc
    29101 gctcccggcc aattcgatgg atgaatctag ataattgatg tacttcttct gcagatctct
    29161 attggcaagc atctccatta aaggtggagt tatactcatt gtcactctga agttcacact
    29221 gtcatctttt aaccttctga atacttgaag caaaggaata tatgtttccg taattgcttc
    29281 aaaaagccac ctttcttcca tgaagcttgg aaaatcagga tggtttatga agggcaagtg
    29341 tgcgtggagc atgagaatca atctgcctct ttccattcaa tcatctcccg ctcttgctct
    29401 tcgagatagt tttaatgaac tcctcagaag atggattgtc tgttggccca gaagacttag
    29461 tcgcgcgttc gaagacgagc tcttcatgag atgctttgta ctctttcctc gatccctttt
    29521 tgaggtctac ccagatttcg taatcggaaa attttggcga atttctaggt gttctggtga
    29581 ttttcgattt caaaacactg ttgaagttgc caccgatgta gtacccaatc tcggcaatgt
    29641 agtctgcatc gggaaaatct acctggaagt accagtttcc ggcatcgggg gagatgttgg
    29701 cctccttgaa tctgtgagcg ttcttaccgt catagataat gtttgtaatg tcactcactc
    29761 tcactagaag ctttgaagaa tgatctgaaa cgcttcttgc agtccagctg gacatatccc
    29821 acatcacgta aacccagtag ggattgaccg ggtgaattac gacaaagtcg cgtgaatacg
    29881 atgaaggaat ctgggcatct acaggcttag atgggcgaag tatttctgag ggactgctct
    29941 ttgcagtggt agtgctcgac gtctgcctgc tcttctgggc aaatttcttt aagacgcgag
    30001 tgatatcctt ctttttcatc gttctcttca agttgatccc catcatcttt gcataacgac
    30061 gaaggtcctg aatcgttgga tctttactaa ggaaagtttg aatctcttcg ttgctcatat
    30121 gaaaaacctc ctgggcatga ttttctacta aagtagataa taacacactc aagggagaaa
    30181 attcaaggta ttataggtga ataacggcga ttattatcat ttttctttat tagacaacat
    30241 aaaatggctg gtttacgaaa tatctcttag tttatcgacc acataatctg acatatagtc
    30301 gctgtctacc tgaaactgtg acccatcgct cataattttg acagtcacaa tgtctctctc
    30361 aatctcttct tttccaacta tcaaagagat ctttgcgccg attcttgccg catgcttcat
    30421 tttgttgcgc atgtttctct ccataacatc aacgtaagtt gaaatgccca tcttacgtag
    30481 tcttctggag tagtcgaatc ccctgatgtt ttcctctctg ccaaatgcca gtattgcaac
    30541 ctgagggatc ttggggacgt tcacatttac cttttcggat ttcaagagca tcactattct
    30601 ttctagccct atcgcaaatc caactgccgg gcagctgttt cctcctatgt attcgactag
    30661 accgtcgtaa cgacctccac caacgatttg gtcctgactt cccagtgatg aagagcggat
    30721 ttcgaaggcc gttctgctgt aataatcaag tccgcggaca aggagagggt cgacttcata
    30781 ttcaatgtca gccttctcta gataccactg gagttcctca aaatggttcc tgcagtcttc
    30841 atcgaggtaa tcaagaatgc taggagcttt tttagcgagc tctttatcta tcttacaatc
    30901 gagaagtctc aatacgtttg tatcaaatct tcgtctacaa tcagtgcaca ttcctggcag
    30961 tttgtcccga taataatctc tgagcgcagt tttgtaatcc tccctgcatt tggggcaacc
    31021 gatcgaattg atgaaaagct ttgtcttact taccttcagc tccttaagaa aactattggc
    31081 catctcgatt atttcaaagt ctgccaacgg actctcggat cccagtatct ccgcaccaaa
    31141 ctgatggaac tggcgcagtc tgcctgcctg aggcttttca taacggaaca tgggaccgtt
    31201 gtaaaagagt tttattggag caccaagatt cactaaagaa ttctcgataa aggcccttgc
    31261 gacacttgcg gtgccttccg gccgcaaggt tatcgatctg ccgcccttgt cttcaaatgt
    31321 atacatctct ttctggacaa cgtccgttga ttcaccgacg cttctcttga aaagctctgt
    31381 agcttcaaag attggagtcc gtatttcagt gaaggagtat ctgtgcgaga tacttctaat
    31441 tgcctcctcc acatagtgcc agtatccgat atcgtcaaag gttatatcct gggtaccctt
    31501 aattctggta atcattatat tatgcattcc tccgtcttct taataggatt ctcccgtctc
    31561 tcatcagttt agttatagca tatattacga ctagaaattc gagccttccc agaaacatgc
    31621 caattgtttc ggcccagatc agcccggcag gggcgttagg agctgttatt ccaaccgaga
    31681 gaccgacagt gctaagggca gatgcatatt caaatgctga aaaagccatc gagtggccgt
    31741 aagccatcaa cacgccagtt ccaatggcat acgtaaggaa atacagggag aaaacgagca
    31801 ggatctcctt gaccagagag ttgtctatat acctcttcgt ttcacccttc cagatttcct
    31861 ctttaaggat cgcatctttc ggaaggaagt accttctaat ttccaaccat aaaatcttta
    31921 agagagcaaa cagcctgtac tgttttaggc ctccagatgt tgaatcgaga tctcccccga
    31981 atatcatgag caaaattacc aaatatatcc cgaaatgatt ccatggcacg aagtccactg
    32041 tagagaaacc agttccagtt aatgccgaaa cagcctgaaa aacactgtgg cgaaactgct
    32101 ctgcgcccga ggagtagaaa atggacagcg tgcctgtcat caataacggc gtgaagatcg
    32161 ccagtatgac gaacatcagc tttggctcac cgttcttcca gaacgctctg aagtttctct
    32221 tccagatagt atagtgaatc ccgaatcccg tagctcctag cagcatcaga acaatggtta
    32281 caatctcgac tgaaacgctg ttgaactctg cgatcgatcc ggcctttgtg gaaaacccac
    32341 cggtcgccag ggcggttaga gaatgattta tggcatcgaa aagattcatc cctgcgattc
    32401 tatacattat tatcccagct acagcatagg aaccatatat tgtcgctatt gtccgcgtcg
    32461 aactcttgat attcggcaca agattgtcca tgcggccttc ggccttatag agtccaagtc
    32521 ccgccggtcc taagatcgcg gacatcataa ttattgcgat gcctgctcct ccaagatact
    32581 gcatcatgct tctccataga agaaaggtgc caggaatcgt ttcgacatct gcgagagtca
    32641 gacccgtagt agtccagccg cttgtagact cgaaaatggc ctgggtaaaa ttgaagtatc
    32701 cggcaaaaat aaagggaagt gccgaaagga ttatcgccat cacccaggag aacaagacca
    32761 ctatggcccc ttcctgaaca cttatcggag atcctggccg gaccttcata gtcagcttca
    32821 acgtcatccc aatcacaaaa ctgatcgaag cagcctccac aaaagcgaga ctctgtagga
    32881 agtcttcggg attccatatg caatacagta gtggaattag cagcacaatg ggaaagaaga
    32941 caaatagatt cccgaggtac ccaaagacaa gcctgtatcg ctgtttcaaa agatttagaa
    33001 acatttacac ctctcccagg agagtttcga aaactgaaga ctgtaccgtt ggactgctaa
    33061 gaactagaag aacatctccc gtttcaatca cagtgttccc tcttgggata acaacttcat
    33121 cctctctgac tattgctcca ataacacttt catcagggag aggaatgtcc ttaatcgcct
    33181 ctccgagtat aggcgactta tcagtcatct ctattcttag aatagagact ttttctgaag
    33241 aagggaggag attcgttatg tgttctctga aaaggctgtc ttcaagcgcg acaccaagga
    33301 tttcagtcgg actaaccaca ctctttatcc ccattttttc gaaaacagct ttgttgtctg
    33361 gatcattcac catagcaacc ggtctctcga tcccatagac gcgcgaagcc gttaaacagg
    33421 cgacgagatt atcctgatcg taaggagtca gagcgacaaa gaggtcggta gggtcaagct
    33481 caagctgctc gagaacgtac ctcttgcttc catcacccct tacgataatt gcacggagct
    33541 tctttgccat ttcgtcacag aacctctcgt tcttgttgac tagcataacg ttgtggcctt
    33601 tggtcaagaa tcttcttgcg agatggtagg caatcttctc accgccaatt agaacaatct
    33661 tcatttgtcg tcaccgccga tgaaatacaa tgcgctctta atactctcaa cagccaggag
    33721 agttggacaa atcacttcta ttccaaagtc cgagaacatc ttgatgttgt taggatcata
    33781 agctcttgcg acaactcggg gtacttcgaa atactctttt gccgacatcg agaccatgag
    33841 atttgtgttg tcgtccgtag taagcacaag ggcaacatct gccttgtcaa gcttggcctc
    33901 cttaagcacg tcgagctccg ttgcatcgcc cagaatattg aagccggtga attcattcga
    33961 cagggcatcg aaagaagatt catttctgtc gacaatgacg acactgcacc ccaaggaaga
    34021 tagccagttt gccattctag aaccaagccg cccacaaccg aaaatcacca catagtattc
    34081 atcttttttt atcgcacctt tcatgaaagg cattttttac tcctctagtt tcttaagctc
    34141 gtcggaagcg tccttaaaat cctcatcgag tgagagcgag gccttcagat gttttatggc
    34201 tactctggga ttgtctttgg ccacaagaag agcgtaaaga aaatgtggaa agggtgaacc
    34261 cggaaagtcg gagaaaaggt ttcccattag ggactccgcc ttcgccgtct ccccttcctc
    34321 aatcagtctc ttgatctccc ttgctcgttt gtagatgctg tcttcttctc taagcgcaag
    34381 ttctgccgca tccctgatct cctgcggcgt aaatggttta gtcaagaaat caacaattcc
    34441 cgctttgaaa gcgctcctca tcgattccgg ggaagtatcc gaagatacta ctattacagg
    34501 tattgaataa cctctatttt taagttcttt tagaaactga attccattca tcgaaggcaa
    34561 actaatatcg gtaaatagga ggtctggctt acgtttctcg atttcttcca gggcttcctc
    34621 tgctgtcacg aaagtatgga cttcgttgtt ttcgttctcc atggtctttt tgacgattcg
    34681 ccttacattt agctcgtcat cgaccacgta aacgagtgcc atgcaatcac ctccataaca
    34741 caaatcatat taccatatta agatgaatag tgtttgatgg ttttcacagc caaaattctc
    34801 tagagattat tcgtctcaaa ctgcttcttt gcgaagtcaa agagaaagga taatgcaaac
    34861 ccggagatta ttaaaggaag attcatctca tcgaatgcca aaaggcaggt tgaagtgagt
    34921 agtccttcaa gccaaatgct gaataaaaca aggacctggt agatagaaag tacatatgag
    34981 aagaaactac caacgagaag atgcgaatac ctctttctaa ggtcgcttct tttctctgcc
    35041 agccctaaga agtagattgc tgagttaatt ccagcaatca ttgccaggaa aggctcacgt
    35101 ttggtcagaa gaaggccgaa aaaggagttt acagaaagag tcgccaaaag agttcctccg
    35161 aacaaaagcc agaaagttat caccctctcc ttcataacaa ccctccaaca actcttacga
    35221 tcagtgcaac cgcatacgcg accgcaagac tgtaaaaagc cgaaaagaag acgaacttgt
    35281 aacttcccgt ttcattctta atcaccgcaa gtgtcggcat acatggtaca taaagaagaa
    35341 cgaacaccag gaaacttaag gcgatctctg gactaatgat ggttctcata atatctaaag
    35401 aagtgccgct cacgccggga ttgccaaaaa aagtcgtaag agaagaaact ataacttcct
    35461 ttgcagctat tccaaagact agactggtta tcatctccca cgtgtatccc agcggaacga
    35521 agagaggttc aagggctttt cctatcgtgg cggcaaaact tcccgttcct tccgtcggaa
    35581 agtatgtcaa aatccagatc actattgaag cggcaagtat tatcgtgccc gccttctcga
    35641 gaaagtgtcg ccctttgttc catgtataga gcattattga ggtcagcctg ggctttctgt
    35701 atcttggaag ttccataatc aacgtcgacg gttcaccttt gaaaaacagc ttgttgatta
    35761 gaagggccga aagcactgtt atcactatgc ttagcagata aaggaagaag acgactatgc
    35821 cggcattgac tccgaagaac gtgcctgcaa tcagtacgta caccggcagc cttgcgctgc
    35881 aagatataaa cggagagacc agaatagtta cgagacgttc cttgggttct gaaatggacc
    35941 ttgtcgacat aatcgatgaa acattacaac cgaagccaag caggagcgat ataaaagagc
    36001 gaccactgag tttcattgca tacataagct tatctattac gaatgccgct cttggtagat
    36061 agccggtttc ttcgagaact ccaagggcaa gaaacagtat gaatatgttc ggcacgaaca
    36121 ctaagattgc cccgactcct ccaattacgc cgtcaacgac aagtgacctg agccagtcta
    36181 tagcgatcca gccgcccacg acacttccaa gaccatcaag tagattttcg atcattatag
    36241 aaagggggcc cgaaatagtg aaggtcagct ggaaaacaat atacatgatt gcgaggaaga
    36301 tcaaaatccc aagaaactta tgggtcatga cgtggtcgat tgcttccgaa aggttccagc
    36361 cgattttctc ctttcccttg acggaagaag agagaattct cttgatgtat ttataacgcg
    36421 tttcggcgat ctctatcgaa taatcatagt caaggcttat gcctgttaat ttctcgactt
    36481 ctttatctct ctctaaaagc tttatggaaa gccaacgctg gtcaaaagcg gaaagagagg
    36541 catttccctt tattctatcg gacaggtttc taagactggc ctctatttca tctgtgaaga
    36601 gataatggac atgactgctg ctccttgcgc tcttaatcag cgtatttttc aattcctcga
    36661 tgccttcacc tgtaacggca gaagttagta caacaggaat accgagttgc ttcgaaagct
    36721 catgcttgtc tatttcaaat cccgcttttc tcgcttcatc gatagcgttg acagccagta
    36781 ctacatcacc gcgaagctcc aacacctgaa gcaaaaggta gagacttctc tcaaggttaa
    36841 cggcatctcc aattacaagg gctacatcag gcctttcatt tatcaggaag tctcgcgcta
    36901 ttctttcatc aaggctggta gccccaagag tatatgttcc gggcaaatcg accacgtcaa
    36961 ctgtatggtt ggcgagcttc attactccgg ttctcttctc gacggtaaca cccggccagt
    37021 ttgcaactat ctgccttgaa cctgttaacg cattgaatat actggtttta ccaacgttag
    37081 gatttccgag aagcgctatt ttaattttct ttgactcaat agcatcggcc tggttttcca
    37141 cgactaagtc tatctctccc taacaataat tctcattgcc tgtccacggg aaagaactga
    37201 acgaataccc tttgcactaa cttcaattct cttccccaga ttaccgatca ccgaaatctt
    37261 tataccttct tctataccga tcatcctgag cctctgcctg aaacccatgc cgccgttaat
    37321 aagacttacc caataatcgc cctctgaaac gtaagagaga gggaaagaag aagtttcgac
    37381 aagaatccac atagaaatat cgtctctaag ggtaatatca cttcctttga ttctaaaaac
    37441 cttaggatct ccgaaaggag ctaccttcac cagctggatc tcttctccag tgacgaaacc
    37501 caaaccgcac agcctctctc ttgtaacagg cggtagctcc agttttctta tcttaccctt
    37561 ctctccttcg cacatgcttg acagaggaac gaccatctac atcaccctta ttgagaattt
    37621 gtctcaacaa cattttatca tcaaacactg gcagctgcac ggtccgatta tgacgaattt
    37681 ctgaagatcg cctttcaggg ctccacttgg ttacccattg tcgttcagtt gaattgttga
    37741 cccttggaaa gctttttatg ctcaaagcca accggatctt gtcagttgct cattgcggcg
    37801 gaagtgcccc ccttgttcct tttcgaaggt tatcaatcaa tagtcctttg agactactct
    37861 gggaagagca tggagacata tgcttagcct acgagagaga agaagaaagg tcgttatcga
    37921 ttcatccctg tcggagaaga tcggttgcga gtttcggtgt gccaagttgc aaaatacaga
    37981 aaacttgatc tcttagcatt tttagttcct acttactcct gaatataaaa gcatttgagg
    38041 agtaaaggcg aaaagtgtaa aatttgctct gaagaacatt cggtttacga cataggtaaa
    38101 gaggagtggt ctaatgcact acatctatca ggttgattca ttcactgctg ttcctttttc
    38161 aggcaatccc gccggagtat gtgttttgga agggcaaata gatgaaaatc tttgcctgaa
    38221 aatctcatcg gaaatgaatc tttcagagac tgcatttgtt tggcctgaag gaacgagtaa
    38281 tactttcaat ttgaaatggt ttacgccaaa gacggaagtc aaaatgtgcg gtcacgcaac
    38341 tcttgcaact gcgtggattc aatttacgga gctgggtatt aaaggcgaga tcttttacga
    38401 aacactcagc gggagattga aagtgaagct tgtcgatgaa atgattgaga tggatttccc
    38461 ttccgaggat cccgtaagaa tcgatctaga cagaaggatc ggggagataa ttgggattga
    38521 aggcaatgcc tattattcgc caggtacgga aaaactgctc tacgaaatcc cggtaaaaga
    38581 tgagcttgag acgctttcgt ttgacaccaa aagacttctg aaaacagatt ttggtaaaca
    38641 atcaaaaggt cttatcgtta cgtgctcggg tgacgagaga tatgactttt actccagata
    38701 ctttgctccg tgggttggta tagacgaaga cccggttact ggatcggcac acacagttct
    38761 tggtcccttt tgggctaaga agctaaacaa gaagaagctg aaggcctgcc agctttcggc
    38821 tagaaaggga gaactcgaga ctgagttgct agacgacaga agaatcctga ttcggggaaa
    38881 ggcagtcact gtaatcaggg gcgtgatttc gatctagtct cttcttgatc taattcgatc
    38941 ctgagctcag cctcatctat cggttctgtg ggattaacgg ttgcgaagac ttttctagat
    39001 gggtcctcct ttacgataaa gaccgaaaaa ctcacaagca ggaggaagat cgctcccagg
    39061 gaaaaaccgg cgaccgttat gtaacttgaa agttcgagat tgacgaatcc cacagcaaat
    39121 gagaaccagg cgaaaacacc tcccgaaagg aggtctgcct tgaaatgggt aagtataatt
    39181 gccggaacta gaagcaaaaa gagaagcagc acggaagcgg cttccgagga aaagaataac
    39241 gagaggctac aaatcagtat aacgagacta aaggaaaagg aaatacttcc aagatagctc
    39301 gatttgatgt gcttcagtat tcgaccataa ctgggtttgg atagcctcag aaactgagcc
    39361 gaaagaaagg agatcggaat cgccatgatc atggcgatac tcttaattcc aactgcagaa
    39421 aagatgatcc agaaagtgaa agaagcgtaa agcgaaagta gccaaacgtt gtcgaaatct
    39481 gccttctcat actttctaac aaactgaaaa tggattacga tgatgatcgg ccagagaaga
    39541 ctacgggaaa cggcacgata gagatatcct tcatgttctg ctatagagcc ctcggttaaa
    39601 atcacaccga gtatgtaaga aagagcccat agggcaagga agatcataat cagggtttta
    39661 gaaattttca gtttgctgcc tccaatccgg cgttttcagg caagccggtc tactctactg
    39721 actctattct gccaagaaca atcctttcga tcctctctac ctttgacaaa gcttcctctt
    39781 tattctttga tctcgagtag atatatattt tgagcttcgg ctcagttccc gacggtctaa
    39841 cggcatacca ggaaccgtcg tcgaaagaga atttgatcac attagaaaag ggaattccag
    39901 tctccgattc tttcccgttc ataatatcgg tatcaacacc gcttaaaaag tcgagatagt
    39961 gagcaagctt cgaaccatcg atttcggaag ggtagctctt ccggtactcc ttcattattc
    40021 tttcgactct tttctgtccc gaaactcctt caagaacgat cgaaatctgc ttttcggaga
    40081 agactccata ttttgcaaag agttcctcca ggacatccag aagtgacttt ccagccttaa
    40141 ggtaatacgc cgccatttca catagaagca tcgaagtaga tacggcatcc ttgtctctta
    40201 cgaagttccc tgctacgtat ccgatactct cttcataacc gaagatgaat cttccctctc
    40261 ctcgttcttc aatatcgaga gccttaccgc aaatattttt gaagccggtg agtgcctcat
    40321 aggtcctgac accaaatcct tcggcaatta ccttcgccat atctcccgtt actattgatt
    40381 tcacaatgaa tccattttcc ggaagcaagc ccttctcact catcgaaaga agaaggtagt
    40441 tgaccagcag cgctccggtt tgattaccat tcaaagcgac ataatcgtcg tcatctctga
    40501 ccattactgc caatctatct gcatcgggat ccgttgcaag caggatatga gcgtcctttt
    40561 caagaccgag tctcttggcg agcgcgaagg cctttatatc ttcagggttt gggtatccta
    40621 ccgttgtaaa atcgggatcg ggccattcct gttcgcgaac tacgaagacg ttcttgaaac
    40681 ctctctcatc cagcacacga cgcacaggaa tgttcccagt gccgttcaag ggcgtgtaaa
    40741 ctatccttat tgacttatcc agctcatctt cggaatctct cagtgttagg gacaggactt
    40801 ttgaaatgta tagatcatcg agctctttgc ccacttctaa gagcaacccc ttctttctcg
    40861 ccacttcaag cggaatcatc tttgacccca cgaataactc ggttttgcgc atttgagtgt
    40921 agacttcatc agcaatctcg ggaagtacct gagcgccatc atcccagtag agcttgtaac
    40981 cattgtattc cttggggttg tggcttgcgg taatcattat ccccgctaca gtggaaagtt
    41041 ggagtacaga aaaagagagg agcggagtgg gtcttatgtc atcaaagaga tagaccttta
    41101 ttccattacc cgcaagaacg gctgccgaaa tctctgcaaa ctcttttgac atatgcctcg
    41161 agtcgtgggc aatcaccaca cccttttctc tgtaagcggg gtctcctgaa agaatgaaat
    41221 tcgccagacc ctgtgttgcc ctcgcaacag tgtatttgtt catccgattg tcaccggcgc
    41281 ccaacttgcc tctcaagcca gcggttccaa actcgagctc cttgtagaat cggtccttta
    41341 tctccgtttc atcgtcttta atcgatatca gttcgttgcg agtatgctca tcgatagtcg
    41401 ggctctcgag ccactttctg taacggtctc tgtagtccat cttaacctcc tcgtccatat
    41461 tcaattatat agcatcagtt tgatcaatat caacgaagcg aaggttgaaa agcgtatact
    41521 atctagtgag atcctcttcg ccttttcagt tttcacagta gtttggtcct attgcaggca
    41581 ccgaccagca ttgtataatc tcagagacga ctcttcacat ttgggggtgt gattttgctc
    41641 tacagaaaga tcatggttgg agaaaatctt gaatctatgc agtgcgaaat gtattttgaa
    41701 agcctgtttg acgttgattt tgactacctt gttggtctag gaaaagagat gccgacgcta
    41761 cttttcatag atgcgagtaa gtacagcgaa gatgtcctta atgactggat ttttgcattt
    41821 accaatatca ttctcatcaa caacaatcca attagagctt tcattattag aggttattcg
    41881 gaagatcgtc aggagttcaa gggttattat cgcgttttga gcagttacaa aagtccgaga
    41941 caaatagtgc aggaagtcac gaccagcatc gatgagaatc tagataagat cattggaaac
    42001 agcaacaggg tagtgcagat gaagaaaatg atcgttcttt cgatgttcta cgacggttca
    42061 ataatgattc ttggagagac cggatctggt aagaatcttc ttgccgagat tattgcaaaa
    42121 ctctccccca ggggctccag acccttctat tcaataaact gcgcggccat cccggagaac
    42181 ctactggaga gcgagttgtt cggttacaag aaaggagcct tcacaggtgc ggctgccgag
    42241 aaggtcggtc ttatagagca ggctaatgac ggaacactat ttctggacga gatcggagat
    42301 atgcccctaa acctccaagc aaagattttg gcgataaccg agaaccgaga gttcttcaag
    42361 atcggcgcca ccaagcccat aaaagttgac gtacgcttca tcaccgcgac caacaggtat
    42421 gacgatgctg ctataagaaa tgaccttaga tacagactat cggccgtaaa ggtcgaccta
    42481 gcgcccctga gggagaggaa gaccgatatt ccccagatct ttgatgcaat tcttgaaaaa
    42541 aagggatatc tcgtgaagtt tgagaacatt cctcaagatc tcaaggagag attcctttcc
    42601 tacagctatc cgggaaatgt gagagagctg atcaatatga tcgaagaata cctggcagta
    42661 aacgatgttg ttcaggcttc agtaaactct tcgatgtcga aaaacgtcaa gcttctagcc
    42721 gaagaagcaa tggtggcaag aatgttgaat ggcacaacat ataaagactt ccttcaagag
    42781 gtgtacaaga gtgtttcgca tgaacttttg gtgcaacgct cagaggtcct tggaaacaac
    42841 atgaacgagc tttcaaagga attcggactg acaaccagaa gaatacgaga tcttcttgca
    42901 gaggccaaca gctacataaa agatcaatct actgaagtcg acgacgagtg aaagggcgat
    42961 ccttcaaaac ctccttatcc tagaatatta atgtataatc tctggagtag gaggtgttct
    43021 tttggcagta cgaaagctca gtcttcccat gggagttgaa gtagccgagc ttttcggaga
    43081 gtacgatcga aaagcgaagt atattcagaa aaaactcgac gtaagaatct ctataattgg
    43141 taacgaaatc tggataaagg gtgaagacgg taatgggatt gacatagctg aaaagattgt
    43201 tgatgaacta gttcaaatga gcagagacgg tcatctagtc gagtggaggg aattcgagta
    43261 cattgtagat caacacctgc agcacgatga agatgatgat agtttcaagg gagtctacaa
    43321 cgaagtaaag gaaacgacat tactttccaa tagaataagg cctaaaacaa tcgggcaaaa
    43381 gaactacatg gagacaatga aagaaagaga gctggtcttt tccatcggtc ctgcagggac
    43441 gggtaagacg tatcttgccg ttgccatggc cgttgacttt ttgaagtctg gcaaagtcca
    43501 gagaataatc ctaactcgac ccgcggtcga agccggagag aaactgggat atcttccggg
    43561 cacacttcta gacaaagtcg atccctactt gagacccctt tacgatgcac tcatggagat
    43621 gattccggcc gagaagcttt cctattacag ggatagctcg ataatcgagg tcgttccgct
    43681 tgcatatatg aggggaagaa ctctgaacaa ttcgttcata attctagatg aggctcagaa
    43741 tacgacctat caacagatga aaatgttcct gacaagaatc ggttttggat cgagggtagt
    43801 tgtcaccgga gacattactc agattgatct tccaagggat tccggatcgg gacttgtcgt
    43861 agtgcagaga gtcttgaaat cggtcgccac aatcggcttc tcctatttaa cagaccagga
    43921 tgtagtaaga aatcctctgg tgaaggacat tataaaggcc tacgatgaat atgagagaga
    43981 agaaaaaact gaagaaaagt gatctctcaa aggtccagtt tcccctcgag agacttctga
    44041 acataaagga tctcactaat ttcatagtct tccctgtgtt ccttgcttta ctaaacagac
    44101 tgccgttcct ttcgcttccc tcaaaacctt ttctgtattt cgttggatcg gcaattcttt
    44161 ggtacatctt tgcggaaagg tccgtgagaa gtaacaggta cttcaacctt catagcgcat
    44221 atagaataac gttcttttct gtagtgtcct tcggtgtttt cgttaattcc ttcgttcctt
    44281 ctttgggaga tgaaacgctt ctgccagaaa tgacacctat cttcttgagt gtttcgatgg
    44341 ttaccatact gataggctat caggaaggca ttggcagcgg gctgttcatg tcgtttcttg
    44401 catctgcaaa tctcgacaac tctctggaaa ccttcatatt gttgtcagtg gtcgtattgg
    44461 cgactgccct cacgacaaga gctatagaga gaaggattga aatagcaaga gcgggattcg
    44521 aagtcagttt gatattcgtg gcgataaatg cgatcaggat gtctcttcag ggtgagtcca
    44581 taaacgcggt ggatcttctg atagcttttg ctaacccaat attatcgtca gttattgcta
    44641 tcggaatcat tccctatctt gagtatgcaa gcagaatcta ctcgaatacg ggtctccttg
    44701 aactgggcaa tctctctcac ccacttctga aaaacctgtc gatttatgcg ccaggaacgt
    44761 attaccacag catctctctt gcaaaccttt cagaagcggc ggccgagcga atcggagcaa
    44821 atgcaattct ggccagagtc gcatcatatt ttcatgacat cggaaagtcc aaacggcccc
    44881 agtacttcgc cgagaatcag caggagggaa tgaatcctca cgataacctc accccctcaa
    44941 tgtcaaacct agtgataaat gaacacgtga aacagggggt cgagcaggca agaaagtaca
    45001 ggcttccgct tcttgttgaa gacgttatca aagagcacca cggaacgaga gtcaagaaat
    45061 tcttctttca caaagctaaa aacattggac aagaagagag aagcgacgat ttcagatatc
    45121 cggggcctaa gccacagttt aaagagtcgg ggataattat gctggccgat tcggtggaag
    45181 ctgcattcaa gagcatcaaa aagcccacac ctgcaaagac ccaggaactc gtcgaggaga
    45241 ttgtcaatgg aatctacaac gagaggcagc ttgaccagag cggccttgat ctggacgatc
    45301 tagagaagat cattgaagcc tttactaggg tcctcatgaa catgaaccaa ccaaggatcg
    45361 agtatccaaa agaaactacg gaaaaggtgg tatccatcaa tggagattca aattcaaaac
    45421 aagacatcga agaagataga agagagcaga atatctaata ttgtagaaaa ggtcgtcaag
    45481 gcagagcttg gagaaaggaa gataggaatg ttgaatctcc ttttcacaga tgactctgaa
    45541 attcgaagca taaataaaga attcagacac agagatgaac caacggacgt cctctctttc
    45601 cagtacgggt tggaagagga gatagtaggt gatatagtaa tctcgcttga gagaatttct
    45661 gaacaggccg gcgagtttgg caactccttt gaagaagagc tctactacat attgatacac
    45721 ggtgttctcc acgtcctcgg ccacacgcac gagggatacg acgatgaagg agaagagata
    45781 ttcagtctcc agagaaatta ctataaacgt tttgttgagg agggatagac gttgtctgat
    45841 tttaggccat tcagggcagt gagacccgct gaaggaatcg agcagaaggt caattgcccc
    45901 ccttatgatg ttgtctcatt tgaagaggcc agagagatcg ggagaaatcc cgagagcttc
    45961 atgcatgtta tcagggctga gatcgatctg ccagtggact cagaccatta cagcgatcta
    46021 gtgtacgaga aggcaagaga gaactttgaa acgatgcttc aaagtggagt cctggtacag
    46081 gaagaagagc cactgtatta tatatactgg cagaagatga aggaccatat ccagatcgga
    46141 gtagttggag gagcaagtgt tgatgagtat cagaaagact tgatcaagaa acacgagctc
    46201 accagacagg ataaggaaga agacaggata aaacacataa aaacggtaaa cgcgaatact
    46261 ggaatggtct tcctgacatt cagatcaaac gagaagctcg aaactctcct acagagttat
    46321 tccaggaaac tacctctttc catgcgtgtt atcgatgaaa acgatgttga acacagactt
    46381 tatgcggtga aagagcggga tggagttgaa gaaattcgag aggcatttaa ggagatcgac
    46441 tgcatgtaca tcgccgacgg ccatcaccgg gcggcctctt cctccagagt tagagaactt
    46501 ctgaagaaaa gcaatcccgg acataccgga aatgaagaat acaacttctt catggcagtt
    46561 gcctttccac atactcatct gaagattatg gcctacaaca gggtagttca ggatttgaac
    46621 ggaatgagcg aaaacgagtt ccttgaaaag gttgggcggg tctttgaagt cgagaatgct
    46681 ccgaaagacc cgtatgaacc tgccatgaga catgagttcg gcatgtgcct tatgggaagg
    46741 tggtacaagc tcaccgccaa aaagggtacg tttgacgaaa atgatccggt ctcgagtctg
    46801 gacgtcgaca tacttcagaa gaaccttttg agccctgttc tcggaatcga taatccgaga
    46861 aaggactcga ggattgattt cgttggaggc attcgaggtc taaaggctct cgaaaacaga
    46921 ataaccaatg gctgggcagt tgccttttca atgtttccca cctcgatgga agagcttctt
    46981 gccgtcgccg atgagggaaa gatcatgcct ccaaaatcaa cctggttcga gccaaaactg
    47041 cgaagtgggc tggtcgttca tttactgaaa tgaagaccgg agcaatcata gttgcaggtg
    47101 gaaccggacg aaggatgaaa tcgtccattc ccaagcagtt tatgactctc ggtggaaaag
    47161 agatgtttgt cagatccgtg gaaatattcc atggatctga ttttgtttat tccacggtat
    47221 tggttgttca cagagactgg ataaacaaag cgaaggaaat acttctgaag aggggactga
    47281 actcggtaat ggttgtcccc ggtggaaaga gcagacagga atcagtatac aaagggttgc
    47341 tctcatgcag agaagtcggt ttagaagcaa taatgattca cgacgcagtg aggcccttct
    47401 tttctcgtag tcagattcca gagctccttc ggcttgtcgc tccaggtaga ggggtggtag
    47461 taacggaagg agtaaacgat acagtttatc ttgtggggaa cgacgaagtt ttggatgtac
    47521 cggacaggaa ctgcttaaga ctggcagaga ctccacagac ttttccatat gatgaaatac
    47581 tggaggccca taaaagggcg gttgaagaaa acatactttc gtctacggat gacgcacagc
    47641 ttttcgtcag atctggtgga caggtgatca ctgtaaactc gatcgctcct aatccaaaga
    47701 ttacgactcc tttcgacttc gatatggcag aactactgat caacaagggg tcatagagtg
    47761 aagaagagag cgatcttctc acatagaaaa ctccggaacg gactcggaaa gagcaaataa
    47821 ctcttcgatt tctccgccga agaaaacact gtcaaagaag agctttaccg cgctgtcaag
    47881 atatccaatt gcatcttcat cgttcatgaa ccttcccgtt tcgccgaaga agaatgtcag
    47941 cggcgaaaga tttctgacca tcgcgaaatc cacatgtctc ataccgcaga tgcgaagatt
    48001 gtaaagggac tcgtttctct ctttcgcaat cgttgaagag tactcctcgc tgataccgtg
    48061 ctcccattct tcagttgaca taagcaggat tttatttgaa aacgcagtgt ccagttcttg
    48121 aaccgaaaat ccctcgagag tcggatcggc aagaaccatg ggaacgtttt tattagccag
    48181 tgcatactga atggcaaccg cacctcctcc cgagtgtcca tacagtccga tctcattcat
    48241 attaagcctt tcgaagaaat ccgaaaaggg gtcttcgttc attccattaa ggtattcgat
    48301 ggcatagttt atatcgcgag ccaggtattc agacagcatt ctaacctttg actctcttgt
    48361 gccttcaggc aatctctcaa gaagatcatt gccgaggaag tagtgtacat ccccgttcga
    48421 aaatgaaacc accgcgcagg ctcccggatg ttctataccc accaccagat agccggcgct
    48481 tgccagtgat tctgcaagag aggtatgcaa ctcgcttata gaagaccatc caagaagcaa
    48541 gattatgatc ggccaggtcg acgagtcgtc cacgggaggc gcatcgataa aggaattcgt
    48601 ttttacccga tatatatgtt cgaagagttc ctcgggatac ccggtcatta tttcgaagcc
    48661 ctgaaaaacc agaggatcgt tatctatcca tccctttctc ggagccgtgg aaacatctcc
    48721 agcgggatac cagacctcga gacggatctt accaggatcg gagtagtcga ttccggcaac
    48781 actcatcggt tgaggattag atacttcata ggaacgaaat ccaacattat gggggccgtc
    48841 gggcaaaggc atatgattca acggaaacaa atagttcaga atcgcgatta ctcccataag
    48901 aataccaaag atcgctacaa tagttgaata gccacccttc gtcttcatca cggaacctcc
    48961 ttgaagttat acgcttccga ctgattcccc ctcttctaaa ttatatcaac tctcttgcag
    49021 agaacagaag aatgcttaca actaagaaag atccatcaaa gtaccactat ctaccgggga
    49081 atgcagactt gctgagtgac ctctgacact tcttcgcaaa gatcattact gtctcatgag
    49141 taaagatcgc gttatttatt ctctaaaagc cagttagatt tatcccaaaa ttctttcacc
    49201 tttccagaga agagaagatt gggaagcaat ttgcaaaaag atccattaga gtaattctga
    49261 acagactgga acagatcaat cgaaatcaaa ttgccattta gttctgagaa tacgtccccc
    49321 gtttatagat taagacaact gtcatcgact tctcggtaag ctgatttctc cctgtatagt
    49381 tatcaaaatg actgtattga agcaaattta cgacacctaa actccttccg agcttttctg
    49441 cggaagcctg aattgatcat ctgagatacg ctaacaaaga acagttatct catctactgc
    49501 tcttcagcca tagcaaagcc atccacgacg atcctttagt tgttctgaag aaaggactgt
    49561 cggagctatc ggaaccttct aaaatgcgtg cagaggatca tccccttttc acgctgtttc
    49621 tgtgtcatgg accgttacgg aaagataagc tctatgagtg cagaaagcgg ggaaaagtcg
    49681 ggaataagaa cgtcggcgcc ggctctctta agtctattga acttctcttt gttccagccg
    49741 aaacctttcc tttcgtcgga agccacccct atggtgaagg ctccggcttc ctttcccacg
    49801 ctaatttcaa ccggaccatc tcctatgaca atcagctcgt cgggttgcaa tttcctatcc
    49861 gtcagtagtc tctgtattac cttcttcttc gagtactcct cgaagctgct caaagctcca
    49921 tagacacctc cgcccatgaa gtcataaact cccagatact tcgactcttc tataacgtct
    49981 tccagatcgg ttcctgaagc aacaagaaga gtaattcccc tttcatgaag tgattttaga
    50041 aattccagtg aaccgcaaac caggtacctg tctcttgaat cctcattgat ccgcttcctg
    50101 actatgtctt tcagatgccc agtatagacc tgcttgtact gatgaggagt aagaatctct
    50161 ttctcagaaa caaagccgta gtcgatcacc agtctttgaa ggccttccat ctggagaatc
    50221 gtctggagtc cggtagtttc ggctatgaag tcattcactt cttcttttat ctttttacga
    50281 gtctcttctt caatctctct atctccggta ataaaatgaa tcatgaggtc tctcataatc
    50341 ggctgccatc cctcccttag aagggaaatg gtaccgtcga aatcaaagag aacatacctt
    50401 gtcttcccag gcaaccttcg ggtattcctg aagatagacg gatcttcgac tctataagca
    50461 ttaggttctt caagctttga gattgattct ctcaacaaca cccccgttcc tttctgagaa
    50521 acacagagcg aagcggcctt tgtggccgtc ttggctgagt ccaataacga gactttctta
    50581 ccgcacaact ccaaagtcaa ggccgcaatg aatgcatctc cagctccgca gacatccctc
    50641 acttctcctt cgagattgaa tattcgattc agtataccgt tattaaagca gaacgagcca
    50701 tcctcgctgg cggttactac aacgggtgaa cccaccagtt ttgaaacctc agcgagatgt
    50761 ttctgaacca gtatttgatc aggacatctg ttattgtcat ctacaaggcc tgctctttca
    50821 aggatctgag aaaattcacc aaggttgggc ttcaacagaa aacctgaaaa ggcctcgagc
    50881 cagtttctcg aatccgcgac gaataatttc tccgggaata gctttcgcaa cacaatcagt
    50941 ttttttctta cctcttcgtc tatacagcct gttccaggaa tttcagactg atcttggaca
    51001 attatggcat caacctgagg aacgagaaat tctaacattt cgacaatctt ccgcctgttt
    51061 tcttcgacta ggccggccgg gggaatatca agccttggca gatcctcttt ccccgtcaaa
    51121 gcattgatga atttcatgta cagaggggta acctgtcttt ccgacttcac aaggaactgc
    51181 gtgcaaatcc cataacgatc gagctccctc tccagttcga cagccctagc gtcgagtccc
    51241 accggggcga ggagaatcac tttcccatca agcgtggcaa gattcttagc cacattaccg
    51301 gccgctccgg gagagtactc ttctctcact cccgtgatcg tcataagacc ggtttcaagg
    51361 ctcggcctcg acagattggg atcgtaatag acatacttat ccaggaagac gtccccgact
    51421 acgagcaccc tagctttcct ttcccaagct tgattcatag cattcccttc ataatcaatc
    51481 tgtagaacga tggcagagtc tctttatgat ttccctttat gtaataacct tttccaccaa
    51541 aagcattcgg gattctcgta actatcgatt tcacatctct gaagtaataa gaagcaactt
    51601 ccttgttatt tttcgaagcc tcatctagat ctgcatctct tatatcaaag ctggccgttg
    51661 tgattgactt tggagctctt ccaacatttg ccgccatcga tattgccttc agaacaactt
    51721 cagcaccaat gactgcagag cctatcacta tacacacccc gcccttttca agctttgaaa
    51781 tttcattcgc gaatatgaga aagtctcttg ccgagcatcc tccaagagac tcaccgtcga
    51841 agtatggtgt catatggtat atatcggttc ccattgctga gtgaacacaa agcggtatac
    51901 tatcccgata cgcattgtac tggactgaga cctctcggta catgcagtca atcttttctg
    51961 gaaaggcttc tccggataga agcctgccca tcgactctcc atatccaaga aagagcttat
    52021 ttccatgaat cagtgcgttg ttaatatact tatttgtctc ttctgcgaag ccgaaaagcc
    52081 ctctagaaag agcattcgga atactttccg aggtccttcc acagtaagcc agttcaaaat
    52141 catgtattgt aaaagccccg ttcaccccaa gggcagtaat gatcttttca tgcataagcg
    52201 acgaaagcag cggtcccatt ccgttcttaa ttacgtgcgc accgctgaaa acaataacgg
    52261 gtaaaccctc ctcttttgcc tcaagcacct tcccggaaag ctcttccagt tctggtgacc
    52321 tgaagccatc agactcattt actatttcct tcatgtctat cagatccgat atcgacacct
    52381 cactaggtct gtccaacaaa gaatacacct tcacattaga agtatcgaaa actgtgtact
    52441 taccctcgta tctcatcgcc gcaccttcca ttctcactaa gctatatcta ctctcttccg
    52501 acggtaatcg cctcgataag ctgacacaat acgtgaccga gagttatgtg cgtttcctga
    52561 atcctgggaa tgtcagtaga gtctactttg aacagatagt cgcataagcc aagcatctcg
    52621 ccgccagtct tccccgttaa tccaactcgc agcgaacgtc tctctatcgc cactttcagt
    52681 ccccttaaaa tgtttgtcga ttgtccgcta gtgctaattg caatcacaat atcttcttct
    52741 gccagaaagg cttcgagctg tctggagaag acctggtcat atccaaagtc gttagggatt
    52801 tccgtgagaa tcgagtcgtt actgggtagc gatactgccc tgatcggtct tctttccaga
    52861 tataaccttc ctacgagttc agccgctaga tgcgctgcat ctgccgcact accgccgttc
    52921 cccatcaggt agacggttcc gccattcccc gttcttctgg ctatcttgcg cgcaacctcg
    52981 tatatcagct cttcatactt gtcgataaac tcactcttta ctcgcatgct ctcctcgaaa
    53041 gccatacgcg ctacacggtt aaactccatc tcctatctcc ttcttcccat tccgcttgcc
    53101 acaatcaaag cggcatagtc accaatcctg tctttgagct ccgtaataac gacccgattt
    53161 ctcgcctcat cgccgacaag cgcttcctcc gctgccctct ttagagcagc ctcaagccag
    53221 attttcggaa ggcgccaggc aaggttaccg agaattatcc tgtcagggtc gagaatatcc
    53281 agcagcacgg cgagaccttt cccgaagtat gctccgcttt cctcaaggac ggtgatggcg
    53341 ttaggatcct tcttcagtgc aaggtgtgaa atatcctgag tagtcgttcc ccgcccaaac
    53401 cgttcaggaa atctgtaatg cgcatatagt gaaattcccg taccaccaca aaaagactcc
    53461 catgaaccgt tctttccgta gagcgacgga ccatcctcgg ctactctcat atgaccaacc
    53521 tctccagcca atcccgtaga tccactataa atattcccat tgataattat gcctgcgccc
    53581 aggccggttc ccagagtcag aaagacaatg tttagaaagc ccctgccggc ccccagaatg
    53641 ccttcggcaa gtgcaccggc tcttccgtcg tgttcgatcg ttatcgagaa accaaaacgc
    53701 tccaccaacg gctgaactat gttaacggtt ccccatcgaa ggtgaggagg gttgaacaaa
    53761 gtacccgttt tacaatccat aggacctccg acggatattc ccaccctgga tggaatcaaa
    53821 tctcctgtaa gaccgatgat ttctgaagag agcctctcga tgaagctctg aaaaccgagc
    53881 tctggttcgg tcgggaactc tgctcttgcg aggatgttga gagcttcatc tcccagaacg
    53941 acggccgttt tcgtacctcc cacatctatg ccaaggaggg ttctttcttc aatcaactcc
    54001 atcgctttcc ttcatgatct cgcctcattc gaacctgagc gtgtggagac cgaattgcct
    54061 gtagggaagc tcgaccttac cgtcaattac cgggagctcg cacactttat cctcgagaac
    54121 gttcgaaatc cagcatcgtt caaaggccag tgcagtactg atctttgcta ctccccgcct
    54181 cccttcgact tcagcgagcc ggagtacgta accgctcttc tcggaatgct tcaaggcaag
    54241 taccttgagg tttctcgctg agatttgaat cacggctgtt ccaagatcta tctttccgtt
    54301 aatcttgatc atctccttgt taaggccctc ggcttcctcg accaccctga cagcatcctc
    54361 tccatcatga gagaacaacg agtaggtgaa acgatgcctt ccctcatctg caaagaaatc
    54421 gggataaatg ggcgatctaa gaagtgtaag agatatatcc gaaccgttgc aggaatgacc
    54481 atactttccg tcgttgagaa tagctacggc aagacctggc tcttccagct cgacccagcg
    54541 atgggccgga aattcgaatc gggcatcttc aaaggtgttt cgaaccctcg tacttctcct
    54601 tgagtatccc gcggagagat cacatcttac ttctctagaa agaatgctga cgggaaagtg
    54661 agccttgaga agagtccttc tagtatgcca gtcgatatcg ttttcaatat ccagtctcct
    54721 tgaagaggca cttaaaacgt atctctgcac tattgagctt ccttcatact cgtatctcgt
    54781 ctgaacaacc ttcctcaccg gaccgctctc aatcgtcctc acgtcgaggg cggtcagtct
    54841 cttgccagat atcttgtaat cgtgatcgat atcccatgca tcccaagcaa ctggaatgtc
    54901 cttgtatacg tagagacagt tccctgggcc ggaggttact tctttttgaa gttgcttatc
    54961 aaaaacggaa acacttccat cggagtttac ttcaacacgc aaaaagctgt tttcgaggac
    55021 gggatcgctt tccatagcgt gttcttcgac ctctccgccg atacactcga aaacctcgct
    55081 cgaaaagggt tgaacatcat gatcatagct gtaaacaaat cttccgtcat atgttctctg
    55141 gcttgccaga agcttgcccg tcccgtcata gagattcatt tcattatcgt caataaatat
    55201 tgatttttga ggataagatc caacattgat tacggtcttg aatgaagggt ttccatcgcc
    55261 ggcagagtca agtagagtac cggttatttg atttgcctct ctgattaact ggccaagttc
    55321 attttcggcc tggatgtgag cttcatttat tgatgaaccg ggaagaatat cgtgaaactc
    55381 gttgtgaaga agtgtcgccc aaagtttcct ggctctttcg agtggatact tttctctact
    55441 gcagactatt gcaccgacga cttccaatct gtatagagtg tcttccgcat ccttatgcaa
    55501 cttctttgtt cttgcctggc tagtatatgt tcctctgtgc agctcgaagt acagctcgtc
    55561 gtcccaaacg ggcagttcca gagtggaagg caactccttg aaatacttgg tcaaggggac
    55621 catttcgagt ttcggcatgc cagggtactc tttgagcctt tcatagtatt cgaccatgtc
    55681 gtctgtcgga ccgccgccgc cgtcgccgta gccaaaggtg gttattgtct tatcgtaaac
    55741 tcttttatcc tggaagttct cccatgtgcc aagaatcgct tctggattaa gcattccgtt
    55801 gtaacccccg agagggttat cgaagaaatg gcagactatt tcactgccat ccaaccccct
    55861 ccacaagaag aggtcgagag ggaaacgatt cttctcattc cacttcagct tggtcgtcga
    55921 aaagtattct attcccgctt ccttcatgat ttgaggaagt atccaggaga agccaaagac
    55981 atcaggaagc catgccacag aagatctcat accgaactcc ttttcgaaga acatctggcc
    56041 gtatagaaac tgcctggcaa gtgattcggc gctggaaata gtgcagtcgc tctcaaccca
    56101 cattccgccg atcgtttccc atttgccctg cgaaatcttc tcctttaccc tctcgaagag
    56161 atcgcgatcg ttctccttca aatccatgta catctgagcc gaagactgaa gataaacgaa
    56221 ttccggaaac ttctccatca ttctcacagc attggcaaag gtccttctta tcttcctctt
    56281 ggtctcatct atcggccaaa gccaggcata gtcaatgtgt gactgaccgg taacatgaac
    56341 ggtgcccacc gcactatgct cgtctctcaa gctggctata ttactcgcga gtttctcacg
    56401 ggccttgatg accgtgtggc gataattgtc ggtcagtctg tttccttcgg acttctcaaa
    56461 ccttggggat tgccagatct cgtttacgct tccatatgta tggtgcccga aaagagtcgg
    56521 attgtcaggg gccgtcttaa agtactgatc agtagatcta ggaagctcga tctcggagag
    56581 gcagtcgctt ataattttca atagcttctc tgagaggaat tcatctctgg ttgcctcaag
    56641 aacctcgatc gcgaccgtaa aatcgagata tgtgcttgat acgaatctat cttgaaggag
    56701 cagttcggcg ccttcaaaga ccggatcggg caagtgagta ccgaacagcc ccttcggcac
    56761 tgtctgtata tcaattacgt ggctcttccc atcagcaaac tccgttaagt ccagaaactg
    56821 atgatataca tttatctcac caaaagaacg gccgtcaatc aagaccagac tttctcctcc
    56881 gggaaccgct ttgaggaaga gcctctctcc ggcttgaggt ttttctggct ctatggaggt
    56941 tctgaaccac accgggaatt ttgtctcatc ccaccggtag cccactgaga tctccttcca
    57001 gcctttctcg ggcggtcttt ccgcgccgaa atcactgaat ctccatccgc caatcgcttt
    57061 tctctcgatc aaagtatacg ggaaaagctc tgctagttcc tttctaagcc ttgtacagta
    57121 ctcatttcta gaatgataca cggtgccccc tccagtcttt cacaacttat ctttccgcgt
    57181 attcgattaa cgaaattcca gctcagccga gctctttcat ggctctatcg agatcctccg
    57241 aaaggacttc aatttcttct agaccggcat gaatccttat cagagataca ttgtcaccgg
    57301 ggtccctgtg agaaacggcg tacggagaga tcagactttc atatccgccc cagctgacgg
    57361 ctctcctgaa aaacctcagg gagttcacga atttctttat cctgtcgata tcccgggtct
    57421 taagcttaaa cgagaagagg cccgatcctc ccctcatctg cttcttggca agcgcatatt
    57481 gctccgagct ctcgagcatc ggatacaaaa cggattcgac ggcttcactc ttctgtagcc
    57541 ttctgctgag ctccatagcg ttttggaaat gcacgggcat ccggatatgc agagtccgga
    57601 tccccctcag aataagccag gcagcaaagg gatcgggaac ggctccagtg ttgagaaact
    57661 ctttttcgaa tattcttctg acatcctcat cggaccctgc tacaactcct cctacgacat
    57721 cgctgtttcc gcccagatac ttggatgcgg aatggactac caggtctatt ccaaaatcga
    57781 ttggattctg gtagatggga gtcgcccagg tgttgtctat tatcgtcttc acacccctca
    57841 tcctggccag tgaagatact tccgtcaagt cctgcagctt aaaagtgaaa gtagtgggac
    57901 tttcgagata gataatcttc gtgttgggtt taagagctgc ctcgatctca ttcacatcgt
    57961 taccttccac ataggtatgt tccactccaa atctggccag atactgggtt acataggtgg
    58021 atgtccagct gtagcagtcc ttgacagtta ctatgtgatc accgctcttc aagaaagcgg
    58081 aaatactcga aacgattgcc gcaactccag atccgaaaag ctttgccctc tccgcgtgct
    58141 ccagcgcagc cagtttctct tcgagaatat ttactgtagg gttgtttcct cttgtgtaca
    58201 gaaaatggtt cgtctcgtct gaaagggccg ttgagaactc gtcgaaaccc ttgaaagaga
    58261 agatcgaagt ttgaaatatg ggaggattta ccgccccaca aggtagatcg tcttcaccca
    58321 gatgatgcaa aatctcagtt atgtacttct tttccatcct caagatcctc caatcattat
    58381 cctttgacag cgccttcggt aagtccggct attatatatc tctgtgcaaa ggcgaaaact
    58441 atcatagttg gtaagagaga aacaacaagt cccgcgctca aaacgtccca gcttatcccg
    58501 gccttagaga tgaacgagtt caacgctacc gggacagtca tcttttcatc ggagaagatg
    58561 aacatcgatg ccagaaaaag ctcattccag atattcacga aggcgaaact gaaaatcgct
    58621 gcaagtcctg gcctcgttag cggaaatacc actttgaaga gcgcctgcaa agtattgcaa
    58681 ccatcgatca cggcagcttc ttcaagggaa accggtatcc tgtcgaagaa actctttgac
    58741 atgatcgtgc tgaaggcaat taccatggtt acgtatagga taaacaacgt ggaaagccta
    58801 tcggtcatac cgtagtttgc cagcatgaca aacagaggag ccatcagaat gaacggagga
    58861 accatctgag tgaaatacat ggctaacaag gtaccgtttc tgaatctctt cgctcgaatt
    58921 cgcgacagcg catatccact gaacatagat attagcatgg cggctaaagc agccgcaagc
    58981 gccactattg cgctgtttcg aaagtaaact ccgaagttag caaagccaaa cagcttcgaa
    59041 tagctttcga aggacagctt tgaagggagg tatttcaaag gaaatgtgta gatctccctg
    59101 gggcccttga gggatgtaac aacaacccag taaagaggta gtagagccgc aatcagatac
    59161 agccctagag cgatgacttt gaagacctta ccgagcacct tcatgactgt cttcatacag
    59221 ttacctcctt acgggaggag atattcagaa aaacaatcgc atacagtaac aacatggaca
    59281 tgattatgaa tcctatcgcg gctccctgac cgtagtcata gaactgatag attctgttta
    59341 tcatgtgtgt cgccagtatc tgagttgaat tggccggccc accgccggtc attccataga
    59401 ttatctccgg gaaattcatt atccacatcg tcctgagcag gaccgtgcta acgattgtcg
    59461 gcctaatgta cggtatcgtg atcttgaaca actgcttaaa cctatttgcc ccatctattc
    59521 tcgaagcctc atacagctca ttcggaacgg actggagtgc ggcaagcaac atgattgcga
    59581 agtaaggaat tccgtaccaa acatttgcga tgatcaccga catcatggca aacttaggat
    59641 ctgacagaaa acctattggg ttactaataa tcccaactct taccagaatg tcattgatta
    59701 tcccaaactg accgttgaac atccacgccc aaatgagacc tatggcaaat cccgacaccg
    59761 cccatggata gaaaacgaga gccgagtaga ttcctctccc cttgaagggt tttctcatta
    59821 gtagtgccag tccgaagcca aggccgaact ggaaaatcaa agagaaaaag acccagacaa
    59881 gtgtgttgat cactattctt acgaaatcga aatttctatc ggttatgatc gctttgaagt
    59941 taccaaaccc aatgaatcca gtatcagaga ggtcaaacat gttatagcgc tgaaacgcca
    60001 taaccccgcc acggataata ggtgtgtaag taaagactat aagaaggatg aaagccggaa
    60061 tcatcattat gaaaaacttc cagaaattct ttctcaaaat caacacctct cagaacatct
    60121 ctctgcctgc tctagaaaaa cggcacggcg ggcaaatctc gaactgtctc tgcgccgtgc
    60181 cgaatcgtca atcagtcagt atctttcagt tttttatcag tcttcccagt actcggccca
    60241 cttcgcggca acggcttcga ctgtggtatc accgaggagc agagattgca tgtcctgctg
    60301 gtgaatctcc ggccaacccg gccacttttc ggagtcaaga ggataggacg cgaagatgta
    60361 cttctccggg tgactcatca tgtaactcca tgccgtatag acgccggaac tgaagtatgg
    60421 atcattctca tatgtggaag aatggattgg aagcggaccg taactcttgc tgaagtatgc
    60481 gtttacctcg gggctcgaaa tatactttat gaactcccat gcctcttctt tgtgcttgga
    60541 atatgagggg attccgagtc cgctgaatcc gtagtccaaa taggccttgc cggagggacc
    60601 taccgggatg ggtaccacag tgtaatactc tctaccgagc atctcatcca caagggcgac
    60661 cgtatccgga tcctgaatga ggaacggagt gatacccgat acaaaggcat tcacctgttc
    60721 gttgaatccc cagttgattc cgtctgcagg gactccaccc tcgaaaagcc tcacatacat
    60781 tttaagaccc tcgatggctc tggggtcatc gaaataggct tctccggatt tcgtcttata
    60841 aatattggtt gcatcgatgt cgcttaggaa agaagtggct ataagatctg agaatttaaa
    60901 ctcccatgat tttcctctga acccgaatcc atactggttc ttttcgggat cagtgatgtc
    60961 gatacatagc tcaacaagct cgctcatagt ctttggaatg taacttacgc cgagtttctc
    61021 cagaatatcg gttcttacga ataaaccctt gatgaagaaa aactgaggaa caatgaatgc
    61081 cgtgttatcg acggttcttg cggcctgatt ggcgacaaag gtaagagtat cattgtgctc
    61141 ccaaccggca aggtagctct cgagattctc tagctttccg ttgttgacga actgtttcac
    61201 cgtataatcc ctgacttcga taatgtcgag tggctggttc gtgttgagca taagagttgc
    61261 cctttggtcc gcctgctcgt atggaggaga tatcaggttg atcttgatat cagggttctt
    61321 agcctcgaaa tctgcaatta tctccttgat tacctgtgtt cttgcaggac tggtgagaac
    61381 ctcgatgaag ttcagagtaa ctacgccaaa cgaggagact ccgataagca ctaccagtaa
    61441 cagcgaaaga actactttcc ttttcactta aattccccct tttctcagtt ttcagtgcta
    61501 gtttgaaagc actttatcca tcaagtaact gaaaatcgca taactcgcag cccccgtaga
    61561 gaatgctcga aactgattcg taaaaccttt tatgagttga acactgcccc tcgaaaaaag
    61621 gttagattca aacgaatcct ttagcgccgc gaagaaaact tttccaaaat ctagaagccg
    61681 cccattgact acaactgctt cgggacctac aatgtttgaa acaagagctg cgatacgacc
    61741 gagttcgtca cccactctac tcagtacaga attcattttt tgcccaccgt tgaatctcct
    61801 gtccaagaaa tcatcgattt tcctgcagtt gactccgatt tccttgttca agtcactgac
    61861 aaccgtatcc agactaaacc ggctattctc aacttggccg ttgcagaagc tcttgataag
    61921 agcgtaactg atctgaccgg aatatccgga gcttccgctg tagatctcgt cgttcaatat
    61981 gatccctgcc ccaacacctt catcaaaata catactgatg aacgatctga tatctctgca
    62041 ttttccaaag cacttcattc cgaaggctgc gccatcggca tcattgacga gaaacacggg
    62101 cagaccaaac ttttcgcggg taatctctcc caccgggaaa tccttccagt cgctcaaacc
    62161 gtagggggcg taaatcacac ctttctcttt gtccagagga gagggagctc caataccaat
    62221 ggcatcgaca tcgatgccat ttctgaaaca tttgttcagc aacctctgaa cgacttcaaa
    62281 gagatgggca gtagcatcag ctctagtcag gttagagact tctttgctca ccgattcaag
    62341 gagcttacca caagctgtca tgaccactcc agacgtgctt ccccttgcaa gattcactcc
    62401 aatagctact gcagcatcgc cccgaactct catagggata ggtgttttgc caactagacc
    62461 cgtcttcaac cccggggttt cttcgacaat attcagatcc agaagcgcgg caattatggc
    62521 agaaattgtt gtcttggtga gttcggtttt cctcgcgatt tcaactcttg aaatatccgg
    62581 atttgcgagc agaagctgta tcactcttcc catgttcttt tccctaagat caccgggctt
    62641 cgttatcact tttgggcacc tctgaaactc aatttgatta tttagtaata gtttcttact
    62701 aaatattata taggacacag tgctgagttc aaagaagaat caagtccaat tattcccgtt
    62761 gagtggcgat tatctcaggc caaaggtgat taaagtcaat ggttgccata gcgcgaaaag
    62821 caatgatgag aaagatgcaa agcacttcta aaaaatatct tttaagtgct ttaattgaca
    62881 atcgtagatc ccctgttggc acgagattgc agcacaggta atgacagaat tggtgaggga
    62941 gtgagtgaac ttaactatga gttgtttcat gtgcagtaga agacgagggt tttctgttat
    63001 cgagatgctt attgttctcg cggtacttgc cggccttatc gctgtcattg cacctatagg
    63061 ggttaatacg cttcgtaaat cacaggccgt ttcagttgca aaagatctta agacgctttc
    63121 tagtgttttt tcaagtaaga tctacctgga tgggggagta cccgtagtta tcggcgaact
    63181 cgggagaaat gttgatggcc gttccttcgg cgccgcatgg aagaagaacg atgatggcga
    63241 gtacaactac ttcgtattca caaatcgcag tgtagacttc gagactgtct cgggtgtcct
    63301 agtcgattca agacagggaa ttccctttgg aatagacgac ttcacttttc ttgacggtgg
    63361 gttaggaaga gaatcacttg actccgaaac tatttactac agtctgctag gtgaaaattc
    63421 catcgccccg ctcacggaat tcggatctac cttcaatgaa ataagcgaag gactgaaaac
    63481 tctcattcaa gagtttttcg tgaataacgg acgatacccc agagattgga tcgataaaga
    63541 gtccggcaaa gactataggt ttgaagatct ctcgcttgac gcttctttct gggctaatag
    63601 tgcatttggt ggcgttgttt ataaaccggc aggtaaactt ctcagagtca gtccagacaa
    63661 tggttataag tttgtcttta actttctggg atcatctgag aagggcgaac ttaccgagag
    63721 gctaaaatgg gacctgattt acaacatcgg aaacgactcc gcagacactg gctgctggta
    63781 ttttcacagt ctcgagggaa gaaagataga tatctcaacc ctggaaatag tgaagctcag
    63841 ctaaatgggt taaatggaga cactaactcg actaaaacta gaccgtttcg gttttgacaa
    63901 aggactgaga tggtgaagca agttcagatt acggtatgcg acgttttaca gctagacaaa
    63961 caaagtcatt cgaacttgtt tcgggatccg ggctttgaaa tacagtcgtc ggggattgaa
    64021 ggttcattat aggaagaaca agagagactg gttggccgtt gagtggttct ccgttctcca
    64081 aagagaaacc ccgttcttcc acagcgagga tctgttcctc gtttctggtc aaagtcaata
    64141 aacacaagat tctgaatcaa gttcagatga cgaaatgttg tgttcttaag aatcacagga
    64201 cgctgtcatg ttcagatgac gcattgttcg attctcttaa cgatcttgat ctttatacct
    64261 ctactccaca catctgacct ctctcattgt tccacccttc gaccttatta cagaaaccac
    64321 gatagccttg agtccattga tgttcttcag taaattgcgc ccaataatct attcttcctc
    64381 gcgccagcga gcgtcggttc ctgccgtaaa taacatcact tttgcctttg ttcatcgatt
    64441 tctgaggacg gtggaccgtt gacgaacaac gtgattttat cagcgttcat ggctctttgc
    64501 ccggcgaagt cggcaatggc ctctgcaaag caaagactgc cgcatgaact gcgacttgcc
    64561 aggtggagcc gactggcttt ctgatcgcat ctagctatgg cataccctag ctccgagact
    64621 ctatgtccat ttttcttggc tctctataac aagttctttc agctccgtgc cgtcgaagac
    64681 tcttatcaag tggttatttg tatcggcaat gtacagtaat cctttgtgaa acttgacgtc
    64741 attgggttca aatagaccga ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    64801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    64861 nnnatgtaca gtaatccttt gtgaaacttg acgtcattgg gttcaaatag accgaggctt
    64921 gaacatttat cgccgttcag tgtacatgta ccctccctta ggttctttat taccgtctcg
    64981 acgcgcctta ttccgagatc gatcttcctt attgcgtgat tgtaagtgtc ggccacgtag
    65041 agaaatctgc cttcaccgtg aattccaata gggtgctgga acaaagacct cttgagaatc
    65101 ccgtccacat aaccgaagga gaatagacca cttccggcaa ctgtttgtgc cgtcccggct
    65161 tccagatcta taaatctgat tgcagagact tcagagtcgg ccacatataa gctgtttcta
    65221 tcgagaaaca aaccgctggg ctgcgcaaaa ttagcgtctt tcaggtgccc gtcgccgatg
    65281 ttttctactc cacttcccgc aaatgcactt atagtgttgt tttcaagatc caatctccat
    65341 atctggtgca ttcccgccat tgcaatgtac aagaatctgc catcagtttg gagatcccag
    65401 ggcgagttaa gtctcgcttt cgaagcgtct ccattgtaat tccaatcgtt gtcctgaaat
    65461 ccgtcgccgg acagtgtact tagacatctt tggttcatat ctgcgatacg taatgcgtgg
    65521 ttctcggtgt cggcaacgta tagtctgcca ttagagtaga ctattccttg aggtttgttg
    65581 aggcgcgcat tttcaaaagg cccgtcctct aagccacttt ggccgctccc gatctgatct
    65641 attgtcttcg ccacaaaagg agtagaaagc tccgttaaga gtatccgatc gttattcgta
    65701 tcgcttatga atagattctc tccgtcgcca aaggcaatct tcccagggaa agagagattg
    65761 gattctctct tggtaagctc tagcttaacc ggaattctct tctcgctaag aactcccttc
    65821 ctctttcctt cttcgagagc ctttgagatc tcatgtgaaa ggtattctcg ctttccctct
    65881 cccgatatct tagacagtat ctttccatca ggcccaataa gaacatatga gggccacgca
    65941 cggatcacat atctttccca gaggaagtaa tcattatcga ttactaccgg atgttggatc
    66001 tcatatcgtt ggactgccgc tcttatgtta cgctcatcat gctcgttttc gaatttcgcc
    66061 gaatggacgc caattactac gaatgggcta tcctcatact ttttttcgag ccatttgagg
    66121 tccggaatga catgaataca gtttatacag cagtatgtcc agaaatcgag cagaaccaca
    66181 taacccttca gatcagcaag ggtcagaggt ctctcgacat ttatccactt gctagactct
    66241 ggaaattcta tcgctcttcc atacaaatcg atcacccctt tcgaactttg actatagaag
    66301 aaggataatg gatcatgctc ctccgttcca atggaaagga tcgcctctgt cattacaaga
    66361 agctttcaaa gcattctatt tgcttgcaca gagtcagcca atgagccctt ctcaatagcg
    66421 ccttcttaca gatagtcttg cagatgaatt ccaaatgatc aggctccttc aggaaggaga
    66481 atcaaggcgg tcgaaggaga caatgagcat aaaaaagcag cgatgttagg cccttgaaaa
    66541 ccttcaattg gagcaatcac ttatgcatta cgcttgtgga gttccttatc gtaagcttga
    66601 catcgataat cgtatcggag acttcctttc cagacagtat ctttgataga gcaaccaccg
    66661 attccgcccc gagggttctg ggatcctgat cgacggatgt aagcgtggga atcgttatcg
    66721 gagcgatgag gttgttgtca tagcccacca cagatacctg atcagggacg ttgatacctt
    66781 gatcagtcaa gaaacctatt acaccagcag ccacaaggtc gttagccgct atcactgaat
    66841 ctacttcaac cattctctca agaagctctc tcgctccgat gtaaccggcc tcgtaatcgt
    66901 atccacatat tacaatgagc gagggatcgt actctatttt ttcctcttcg agagcccttc
    66961 tataccccat gtacctgtcg agcgtcgtgt atttgccggc atcctcgctc gaagccactt
    67021 ccccaatcgt gtctatggta catgggctgg accagaagcc ggcgagaaag ccaaccctct
    67081 ttcttcccag tctgagcaag tactttgtcg caagataacc gcccttaacg ttgtcagtat
    67141 aaacatgagg cttagacttg aagacgtttc gccttccgac aactacaatt gggagctcct
    67201 tgaaactctc tatttgcgga agaggatccc cggtggaaac cggacagtag atgagaccat
    67261 ctacaaccgt tcgagagaga ctgtcgatcg cagctttttc gagttccctc tttccgtcgg
    67321 aagtggagat aattattaca tgatcgatct ctccggcctt aagaatggct cccgcaatca
    67381 tcgcgctgaa aaagggttgg gaaacatccg gaactagcat acctatgaca tttgtggtct
    67441 ggcccttcat gaatctggct ccaacatttg gtttgtagtt gagctttttg actgcatccc
    67501 agacttttac cttcaagtct tcttgcacag gccttgaacc atttaaaacc cgagaaaccg
    67561 ttgcggccga gaggccagcg taatcagcaa cctgttttat ggtaaccctg tcgctctttc
    67621 ttacagcact tcacctcctt gaccggaaaa ggacttgaga aaaggcaacg ccactaagct
    67681 tattataacc agaagagtaa ctatgccaaa agtcgaattg aaagctgcaa ggtcaatctc
    67741 cgctccccca ctcgaagaga aacgctgcat caggaatccc gtgccagact gaaaaatgat
    67801 tacagagatg aaagtgaaga ggttcatacc tccaaagaga gaacctccac ggcctctggc
    67861 tgccagatcg ttgacacctg cagtaagatg atcgtaggtt atacccgcaa agaatcccag
    67921 aaagaatccg accagtcccg gtataagaat cggcagccgg ctcctcatag aaaaccagaa
    67981 gaaaacccac gaagcaccat aggaaacaca tgaaacggtt atcgcaggga gcctcaatct
    68041 tgccggcctt gcgatgtatg cccctacaaa gtttccgacc atcactccga tgcttatcag
    68101 cgtcgaccat aaactcgccg t
//
LOCUS       DAOQYI010000008        63845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_483_length_63845_cov_81.678743, whole genome shotgun sequence.
ACCESSION   DAOQYI010000008 DAOQYI010000000
VERSION     DAOQYI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 63845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63845
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_483_length_63845_cov_81.678743"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKL93_04075"
     CDS             <1..226
                     /locus_tag="PKL93_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70471.1"
                     /translation="VLLNVALFPVFIFGCGMSIVGAAVASVISQSVSTMIILEYYIRK
                     KSSTNLSIRLYSLSLFRYTLKSSKSVFRHS"
     gene            435..890
                     /locus_tag="PKL93_04080"
     CDS             435..890
                     /locus_tag="PKL93_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70472.1"
                     /translation="MLIVITVFAVVFAIIGLTIPSSIISISCNDPEIIDAGSRAFRVV
                     NIFFSTFGFVITSTDLLQVFGKGIAARLLPMSKQDFFLIPAIIFLQKIFELNEDHFSR
                     TVASYFALFVAAFLSVAIFKKLKSQSRELSLRERQPVIIRISPVHDHLL"
     gene            complement(856..1761)
                     /locus_tag="PKL93_04085"
     CDS             complement(856..1761)
                     /locus_tag="PKL93_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HNQ70473.1"
                     /translation="MKAIIVHGGTGSFKSVTDADEHRRGIEEAVRQGFLTLEKINSAE
                     EAVVTAVSVMEEDPTFNCGRGSVLTYRGDIEMDAAIMDNNLNAGAVSGLKRILHPITV
                     ARAVMEQTDHVLLAGAELEEFVTVLGFPREDNLIVPKRHLQWKEELEKIARGEKTRFG
                     KSVKLAKKAEEYHSTCGAVAIDDHGRMAAGTSTGGMMMKSFGRVGDSPIIGAGTYSDS
                     FGAVSATGHGEKIMKLTLSRLVAFFMEQYPAQKSVDIALERARYFDCECGLIALDRYG
                     NIGIGHTSKDMSWAFIKDGHAPVIF"
     gene            complement(1924..2790)
                     /locus_tag="PKL93_04090"
     CDS             complement(1924..2790)
                     /locus_tag="PKL93_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNQ70474.1"
                     /translation="MVSEEITKIKDDLSPAYKRIATFILKEYSQVGFMSIEELSSMAG
                     ASKASVVRFSRRLGFSGFNELKKTIQHELRRRLLPYEKIATTELDRAPVENQLKLLRQ
                     NEINNLKNTLYGIREEELHKWVDTIRAASNIYFGGFGATKHVISLMRYTISVLQRKPT
                     WLLSGSVSDFSFKIKLMERSDALIVMTFPPYSKEVEYMVDFALERNVRTLLVTDSVDC
                     PVYSKAYSSILCENNSLLFGNSFVGPIAVAEMIGNFIILSEKKTGMEEMKRLFEVEER
                     GYKAIKIDGKNK"
     gene            3111..4091
                     /locus_tag="PKL93_04095"
     CDS             3111..4091
                     /locus_tag="PKL93_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70475.1"
                     /translation="MKRVLISVIFLVFFASAFANLVVYSSVDEANARKILNAFVEDTG
                     IEVDFVFLSSGPALARIEAESSNPQADVWFGAPLENHIIAKERGLTQAYKTLSVYGVS
                     PEFYDVEGYYHPIYMNPLGVGVNTSVLEQIKASLPESWEDLLASEYRRMIQYPNPQSS
                     GTAYSLITGLVKIYGEDGAFQYLKNLAPNIQTYTQSGTGPSKAVGPGQVALGIQFTPA
                     FFQFMEQGYPVSVVFPKEGVPYEVASVSILKGAKNLADAQKLIDWVVSKAGQQQIVDQ
                     KTYFYPIRPDVDFGSLQPLSTINLLSIDPAWAAENKTRLIERWINEVLPY"
     gene            4151..5845
                     /locus_tag="PKL93_04100"
     CDS             4151..5845
                     /locus_tag="PKL93_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ70476.1"
                     /translation="MAIRGEEAKNRFLNLLKDPVLLLIIVLIFAALAIFIVYPLFRVF
                     AVSMTDKEGGFDLSAYSHAFSNRYMRQGFFNSLLVAGLTAIFGMLVGYLFAYTLNRTD
                     IPLKGFFRTVAVLPIVFPPFIGALSIIMLFGFNGIITAGIFGIRNFPVYGLWGLMMAQ
                     VVCFFPVAFITLDGVIGTISPTLEDAAFNLGANRWQAFRKVILPMSVPGIASTMLVLF
                     IESLADFGNPLILAGSKFPVLSVQAYLQITGMFDTKGGAALAVWLLFPSIAAFIIQKY
                     WVGKKKYVTVTGKPTTSVLKSVSKPVKWILFSLCMLIAVFTLTIYATIFWGAFVKAWG
                     MNNTLTLENFKYVFDVGLEAIKDTLVIAFTSTPISAVLGMIIAFLLVRKVFPGRRVME
                     FTSMLSFAVPGTVIGIGYILSFNKPPLLLTGTLAILVLNFVFRYIPVGIQSGVALLNQ
                     VDPAIEEAAYTLGADNRQVFTKVTLPLIMPAFFSALVFAFVRAMTAISAAIFLVSARW
                     NLITVQILSQSDSGRLSEACAFSVLLIGMIMGFILILKIFLKNKISLSSGGTAGQG"
     gene            5850..6929
                     /locus_tag="PKL93_04105"
     CDS             5850..6929
                     /locus_tag="PKL93_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70477.1"
                     /translation="MSLILKNVRKVFTSYGTETVAVDDFDQEIRRGQLVTLLGPSGCG
                     KTTTLRIIAGFEVPTNGRVLLDGKDVTNLPPNRRNISMVFQSYALFPHLTVEENISFG
                     LKLKRLDRSTMKKKIREMTDLVGLKGLEKRRPDQLSGGQQQRVALARSLVMEPSVLLF
                     DEPLSNLDAKLRESMRLEIRRIQQEVNITSVYVTHDQVEAMSISDIIVVMNDGKVMQI
                     GSPFDIYARPQNSFVADFIGRVNFIDGEVESINGDSVTVSSSSLGKKFVGRKGGELSP
                     GDGVRIVLRPESLSDREEDKINVLSGKVLKYVFLGSNVEYEIELPDGKILNAVTFNPI
                     ERKFPVVGKETDLYFSKESAWVINN"
     gene            6949..7863
                     /locus_tag="PKL93_04110"
     CDS             6949..7863
                     /locus_tag="PKL93_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70478.1"
                     /translation="MTNKCMDMKKAFLSEEVEIKPYCYRAYDQGPYNSSKDGFEDYLG
                     RPYLSRSDEELYLPFFDNKAPRSGRAPAWQILTVYSYEPDFGMDEGLSLSPLQKFMGG
                     SGTWRHEEFHIILRFGEVTDRFLHFDRMSMLANEKGDRYWALRFAARALHYLEDCGTP
                     YHASPGTPIEIFKIPFLYRKQFKKIGFYHKFHDRYLGYRLWRGYEPFLEEIEGTEAGV
                     FDRLEKMAKTIRRRAFDLLPEVELSVKRLIGGNSFENPSPELSKEYYGKLVSSQDTRR
                     LDEVSCKILRNLSSGVKFYLNHLERRLN"
     gene            complement(7906..11064)
                     /locus_tag="PKL93_04115"
     CDS             complement(7906..11064)
                     /locus_tag="PKL93_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ70479.1"
                     /translation="MSTEEGSDNLARSVFALQERMKELDCVYRIFNELHKSSTADEAV
                     VSALEQLPRGFRFPELTAIRIEIDGNEHRSVDQWSDERMISAEIVVNGKKIGTISVSL
                     TEELDIKGSPFLKEEKKLLSGVAVALSNTLERVIIQRELKESLARLTFAVDASGEGIW
                     DWDIETGKVIFSKRWKEILGFSDEEISDHFSEWEKRIHPEDVEEVKSVLNRCLKGDIP
                     FYEKEYRLIGKNGSLVWVRDRARIVSRDSEGKPLRMVGMYTDLTQRRMDEIKLQKLNK
                     LLTLMFRANEFLVHAKDEHSLLKGLCDIVVENGGFPLVWIAFKKSGGKMLTPVAVSGV
                     AKGYPYEVAVAWDNSELSRSAIGKAIKTGRTFISRDIDSSPDYLPWKKTILKYGLKSS
                     IAVPLVIGKEIIGGFVIYSTEEDAFDNEEVLLLEELGEDISYGILSLRLEAQLEHQSG
                     VLSSIRRVNQLIVSATDVEELIKRATELMTGSRGFERCLLVLVENGDVKNWAASGFSR
                     TQLLEVDSIFESGEIPPLLRRTMEEEGVISLTGRKSSKQCVTISDSDDLAVFAKGLRI
                     GGNILGAMAVVMKQSFSFSKDERDLFSEIVDDISFAIHKIRIEKNERVLQKRFSLFMD
                     KLPGAVFISDSDLKTSYFNDFAARVFGFKEDWKGKNPEANYPGELDKLILQDDRRTQN
                     GENIVREERVLGTDGKERILYVQKFPIVSEGDKPMVGAIAMDITEWKLAEMALTRLGR
                     VIDLSVNELYIFDSETLRFLQANKGALENSGYSLEELRKMTPLDLKLEMDKRAFEDIL
                     FRLKREPGSYEVFETVHIRKDGSKYPVEVRLQLHDNEYERVFVAIVLDISVRKIAEES
                     VKKSLESLINTLSSLVEARDPYTSGHQKRVSELSTAIARKLFSEDEMHEDIVESIRVA
                     ALLHDIGKSAVPTEILTKPVRLNNLEYDLVREHSKKGYDILKEVYFPWPIADIVAQHH
                     ERLDGSGYPRGLTGDEIRIEARIIAVADVVEAMSSHRPYRASLGIEAALDEIRQNSGR
                     FYDRRVVEACLELFEEGFQFTE"
     gene            complement(11207..11842)
                     /locus_tag="PKL93_04120"
     CDS             complement(11207..11842)
                     /locus_tag="PKL93_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ70480.1"
                     /translation="MTNRKAAALRVIVEVGFLFLFFFLLRSRDLQKWFLIFGIGLVGA
                     LFLGRFYCGWICPMETLFRPINWLYSKLRVKRLKTPAIFKNNVFRWSILVLFLLLMVA
                     VRIFKIKVNLLLYITAFSVLITLVFEEEFWHRYMCPFGTLLSLFSRKPLFGMKVEKPP
                     CVSCGICQNVCPVSAIETEEDGMKIDTSECLVCLKCKESCPKISIKYSVLK"
     gene            11986..12765
                     /locus_tag="PKL93_04125"
     CDS             11986..12765
                     /locus_tag="PKL93_04125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ70481.1"
                     /translation="MRKIDGSEWRNLLPFLYKNREFSMFIIGDIENTSPDSDNMEIYL
                     DGDFDSPRGILLRYYKFLILSDGAGMNFREAAGVVRRYEQAMMLSGTISGIDRISPYL
                     QEIREEEETLRFAVLREPNLNAAGFEVRRATTEDAEKLLAFLFSIEEFHATDEESFMA
                     TLKDGSTRRYIIEDQGEILATAASTSESSDMAMIIGVATGKDFRGRGLASAVVSKLCE
                     DLLAEGRTPCLLYDNPEAGRIYNRLGFREIGKLKTLRFKKR"
     gene            complement(12769..12993)
                     /locus_tag="PKL93_04130"
     CDS             complement(12769..12993)
                     /locus_tag="PKL93_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70482.1"
                     /translation="MIISAVGRGCAGAIFSDAFDLAAIAGMLLEIKWSLNTSFKAGFQ
                     LWMSLGLYQRITKPETSFVPMGQIGVALDF"
     gene            13359..13772
                     /locus_tag="PKL93_04135"
     CDS             13359..13772
                     /locus_tag="PKL93_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNQ70483.1"
                     /translation="MEPSEMSAREVLKAFFESYRNQDFESLKSLCTDNITYINPEGLS
                     NNGIDEFLQLLKREFESFGVLFEPISWESSVERPSFCYFSWKRGVKFARKAALRRVET
                     FGSAFLLKVEKKWQLVHFQQAFSGSYASLFRTREK"
     gene            13918..15162
                     /locus_tag="PKL93_04140"
     CDS             13918..15162
                     /locus_tag="PKL93_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNQ70484.1"
                     /translation="MDNTKKAWLVVFSGLGVNLTLGVLYSWGIISAALIDDFKWTATQ
                     TQIPYMLASAVFALTMIPGGKLQDKFGPRPVLFVSSILAGLGFFFSGLNLSVAGLTFF
                     FGLVFGLAMGFGYSSPTPAAVKWFHSDHRGLISGIVVSGFGLAPVYIAPLTSGLIGLF
                     GLPTSLMILGLLFFLIVFTLSFFISNPQEGVEKVRLKKKPRKVHRLTSKDYTLKEMVR
                     TPQFYILWTMFFFGTFAGLLIIGQMSKIGLEQASISNGFLLVVVYAIFNFIGRVTWGS
                     ISDFIGRTATLFAMFAIQALVYFLFSSLTNPLALLIGKSVVGFTFGGMLAIFPVVTAD
                     FYGVKNLGVNYGVMITAWGVGGVIGPLLGGIARDITGGYEISYIVSAVLSVAGALLSL
                     VIRHPEEEKREKNGGRADAKEN"
     gene            15146..15496
                     /locus_tag="PKL93_04145"
     CDS             15146..15496
                     /locus_tag="PKL93_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70485.1"
                     /translation="MQKRTEKLKKIRKTLAVLSDDVQWKLEDVVLTIGDLIDEDKKVT
                     IDVLREIGSMDSRRKKLFMLYMKASEERDALMKQRIEDLWSVEDDELEEVLKELSKHS
                     IDDGKTPKEPPSVD"
     gene            complement(15493..15774)
                     /locus_tag="PKL93_04150"
     CDS             complement(15493..15774)
                     /locus_tag="PKL93_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HNQ70486.1"
                     /translation="MDNHSHHKHSGALKILRTARGQIDGIIKMIEEERYCIDISTQLL
                     AVISLLKKANTTVVNKHVETCIREAIDSGNVDEKIGELETLMKYIEKSL"
     gene            complement(15776..15982)
                     /locus_tag="PKL93_04155"
     CDS             complement(15776..15982)
                     /locus_tag="PKL93_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNQ70487.1"
                     /translation="MRLTIEGMSCNHCKMRVEKSLKALEGVKDAEVDLGTGVATISTS
                     KEIEDRLLKEAVEDAGYLLKGIER"
     gene            complement(15997..18438)
                     /locus_tag="PKL93_04160"
     CDS             complement(15997..18438)
                     /locus_tag="PKL93_04160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNQ70488.1"
                     /translation="MSFENNHVKEEYIVEGMTCAACVRAVEKAVSKIEGVERPVVNLA
                     TERLTFEVSQEVDKGRVFEAVERAGYSLKEIPVFRKAVMEIDGMTCAACSAAVEKAIG
                     RLHGVENVSVNLSSDTVSFKYDPNILKIGEVKKVVEKAGYRPGNLVTEAFEESRLKKE
                     AAIASYKRKFIFSAAFALPLLIIAMGHMMGLSLPAIIDPHLNPLNFAIVQLILTIPIV
                     VAGRDFYSKGIPNLFRGSPNMDTLVGLGTGAAFSYGIFATVQIALGRHSYVGDLYFES
                     AGVIIALISLGKYLESLSRGRTSDAIMKLMNLSPETALVKRQDVFEEVAIEEIEVGDV
                     LLVKPGMRIPVDGKVLNGASSVDQSMITGESIPVDISESSKVIGGTTNINGAFEMRAT
                     VVGSDTLLSRIIKLVEDAQSSKAPIARMADVVSGYFVPFVLVIAAVTFLVWMTLGYGL
                     VFSMSMMIAVLVIACPCALGLATPTAIMVGTGRGAEMGILFRNGEALETTHKIGAVVF
                     DKTGTITEGRPRLVDVHTFGKHERERVMQISASIASKSSHPLDVAISGAYSGPLLEVS
                     SFEAYSGKGILARVSGQEVKMGNHRFISLTPEEMKFVDELSSEGKTPVVVSIEGRAAA
                     VLGIADVVREDSLDAIKSLKKMGIDTYMMTGDNSRTAMAIANQVGIENVISEVMPEDK
                     AKKVSDLKREGKTVMMVGDGINDSPALAEAHIGIAVGSGTDIAIETADVVLMSDNLSN
                     IFKAIKLSKETIKNIKQNLFWAFFYNVIGIPLAAGLFYGVAGLRLNPMVAGAAMAFSS
                     VSVVTNALRLKKIKI"
     gene            complement(18440..18634)
                     /locus_tag="PKL93_04165"
     CDS             complement(18440..18634)
                     /locus_tag="PKL93_04165"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNQ70489.1"
                     /translation="MMFFGFLIVIFAVAFFLKPDILKSRMTISSSYDKEMRVLRERLA
                     NGEISIEQYEQLKKALSEEK"
     gene            complement(18867..19397)
                     /locus_tag="PKL93_04170"
     CDS             complement(18867..19397)
                     /locus_tag="PKL93_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNQ70490.1"
                     /translation="MRLEGKKIAVLVEDGFEDLEFWVPVMRLREEGAEVVIAGREKGI
                     LFLGKSGLEAFSDSDFKSIDVSKMDGIVIPGGWAPDRLRRFEEVKSLVRDANERGKVI
                     GMICHAGLVGISAGIVRGSKAVGSEGIKDDLINAGSVWVDEPALVDGNLVWGRVVKDI
                     PDFCRKLVETLENGLR"
     gene            complement(19414..20331)
                     /locus_tag="PKL93_04175"
     CDS             complement(19414..20331)
                     /locus_tag="PKL93_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ70491.1"
                     /translation="MKKQSLLSALLIMLMISLSISCFATSIDSNHIVTESEVHFFVEG
                     ERINGILTRPESSEGPVPVVVLLHGFLGHMDDLTVYGSEESLYRMTARLFAEKGLASL
                     RFDFRGSGTSDGEWKDTTFTKQISDAISSIDFLSLAEDLDSRRVGVVGLSQGGLVAAC
                     LAACDSRVKSVALWSAVAIPVHTYSALLGKDSVDRAIEADPLEEITAEISWGGTTVLR
                     KEFFDELFLIDPVAEIVSYDGPLLVVSGSKDDLVFPQPEVSNLFTMYHKGVDRLLQQD
                     SGHIFDLFERQDKVLEIIDATLEWFLITL"
     gene            20513..22690
                     /locus_tag="PKL93_04180"
     CDS             20513..22690
                     /locus_tag="PKL93_04180"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNQ70492.1"
                     /translation="MARTEIDAQILLGDLESILQYRLKRRDKERIFDAYEFARFHHQE
                     QMRDSGEPFISHPIEVAKILAQFSADNDTIIAGILHDIVEDCNVPLSEIAEKYGDTVA
                     LIVDGVTKISNLKLNEKLESKIVKSRMKVETLRKMLLALSDDPRIIIVKLADRLHNMR
                     TLDFLKDPEKKRDKARETLQIYAPIAHRIGMHKIQAELEDLSFKYEYPEEFKELKFKV
                     NRKLKERQDIMDEYKEIVLRELRKNRISALIEGRVKHLYSIWQKMIKKNRSFDEVFDL
                     IALRIVTGDEVNCYKILGAVHSLWPPMPGRFKDYIAAPKSNGYKSLHTTLITHRGEPL
                     EIQIRSERMHKEAEYGVASHWVYKEGIDVKDRTWFTQLVDWQKDFIESFKDMESISRE
                     LEVEEVFVFTPKGEVVHLPKGATPIDFAYAIHTEVGHHYAGAKVNDRLVPVNYELQLG
                     DRVEVIVNKASEGPSLDWLKYVRANSTKAKIKRFFKNEYSAKLVERGKEIFRKISKRL
                     AVSMDDLIEGEQIKALMNRLGAHNENDLFSKLGDGSVTMGEVLSLLAPKEEEIETLPE
                     ETELIKQKQAKGNEVVVGGETGIAVYFAKCCTPLPGDDIVAVMSSRGISIHTRNCRNL
                     KGISKDKLVEAHWNLDTGGKFSSWIVVEFDSADRTIVNKSLERLENKNAKVMKYSVEA
                     GRWGYDTLIANILVKDVAHLTSVMENLRGMKGVQNVKRYGGVS"
     gene            22687..23133
                     /gene="dtd"
                     /locus_tag="PKL93_04185"
     CDS             22687..23133
                     /gene="dtd"
                     /locus_tag="PKL93_04185"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNQ70493.1"
                     /translation="MRAVVQRVSSASVVVDGKVTGKIDKGLLVLVGIGREDDERDVEW
                     LVDKTVNLRIFEDENDKMNLSLIDVSGSLLAISQFTVMGDARKGRRPSFTDAAEPEVA
                     RGLFNDFLQMASKTVRVETGIFQAHMDVELVNSGPVTILLDSKRVF"
     gene            23145..23642
                     /locus_tag="PKL93_04190"
     CDS             23145..23642
                     /locus_tag="PKL93_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNQ70494.1"
                     /translation="MKRSLRFILIVIMVSAVGAIVVSEGGAGPSEPAKIAFANMQKVL
                     EATKDWVTLNIDYQKDTKFYQGQLDTLSQEYQDLANSGASQDALLQKQQEILTKKSQY
                     EQTLETTYNAKLQVIMEQVNKRIKDYATFVGIDMVLSSEVVVYGSSAYDVTDAIIEYM
                     KGFQN"
     gene            23639..24361
                     /gene="lptB"
                     /locus_tag="PKL93_04195"
     CDS             23639..24361
                     /gene="lptB"
                     /locus_tag="PKL93_04195"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNQ70495.1"
                     /translation="MSDPSYLKCEHLDKRYGKRRVLKDVSLEASSLEVVGLLGPNGAG
                     KTTAFKSILGIVIPNSGSIFLDGENITHLPIHERAKRGIAYLPQETSIFRGLKVIENL
                     TMVMKLTGQEDKCLEKASEILDEFGILSLKDQTASLISGGEKRRLEFARTMTLSPSFI
                     LLDEPFVGIDPMTVKDIQKMIRKLKSRGIGVIVTDHDVSSIAKVVDRLYVLYKGEVIS
                     SGDPAAVLGDSLVVEKYLGSDE"
     gene            24358..24888
                     /locus_tag="PKL93_04200"
     CDS             24358..24888
                     /locus_tag="PKL93_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HNQ70496.1"
                     /translation="MMLHVTVIGYSGSISASPVKELHGICEEVGRTLARHGHVVMTGG
                     RDGVMELVSRAVSKEGGRVIGVLPAGDEGNTHNEIRIRTGMDFALRSLILTKSADVVI
                     SIGGQAGTLLEIISSYSYGHPVILMGGTGGWTDRIVSVLIDGKYLDERRTSEVKRANS
                     IEDLERLLEEAEDGKV"
     gene            24875..26296
                     /gene="gltX"
                     /locus_tag="PKL93_04205"
     CDS             24875..26296
                     /gene="gltX"
                     /locus_tag="PKL93_04205"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNQ70497.1"
                     /translation="MVRCRFAPSPTGNLHVGGVRTALFNWLFAKNQNGSFVLRIEDTD
                     TERSEKRFEDQILSSMKWCGLNWSEGPDIGGEYGPYRQSERVALGFYDKYAQELVERG
                     HAYYAVYKKSNQEDVLRISMETPDISGDEVYTIKFRIPEGKTEFSDLSKGKMSFENEN
                     FSDFIIIKSNGFPTYNFAVVVDDHMMDITHVFRGEDHLTNTPRQIMIYRALDWEPPTF
                     MHIPLILGSDRAPLSKRHGHTSVDHFREEGYLSVGLMNYLALLGWTVDEEIFDYHEKV
                     KDFIPSDISNKGVVFDYEKLEWINGKHLRLLDPEELMIQFGLWLKFTGRFDYYAIIES
                     DQFYSREVLTMCREKINTLEQLFDFSAPFFSDELDYQDEYVSKYLQTNWSKDLISAAI
                     RKFEDSLDWSVEGVEKTVRELADEKITSKKNTFQTLRGGVTGRLVTPGLFETISVLGR
                     DRVLERLENLLEMIEYEEGNLSD"
     gene            26271..26687
                     /locus_tag="PKL93_04210"
     CDS             26271..26687
                     /locus_tag="PKL93_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ70498.1"
                     /translation="MRKGISLIEMVVSLAVSAILLISVFKIVNMSIAMSFNIVRETKS
                     YMSLSRTFDVIERDLNRSIGNFSWGTSFISFDAIVDGRHKRIRYYVSGDRIIRRSGNS
                     YNTVTEFVKSAGFYGEGGSVRFFVDEGEILIGIEGG"
     gene            26689..27966
                     /locus_tag="PKL93_04215"
     CDS             26689..27966
                     /locus_tag="PKL93_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ70499.1"
                     /translation="MNPKDIRKMLQAIEEEIFSRAEMNVKDMLKSPSAYVRARAIKSA
                     AERDMKIENIESFLDDPSPDVRKNAVSSMAKFGEDRDSILKALDDPSDLVRSATVKEL
                     VEFGYEDEELARKIAADPSKKVRKTFVISLAEAGESELLQEFNGDPSTDVRRILSAYN
                     GELQLDEEVLSSLSGKFQKLAIHSRLGKLDSTSSEKLLGLIKDFRISKTKQILVESLE
                     PFPEEVSVPALRKLVESEDSNVAITALKTYRKIKGYDISLLQAAERFMDSDDEEMRFV
                     GAQYVKGLLEPSAVDILREGLNDPSDRVRAICIEALANLMDNSISDVIDESLRSTSSR
                     LKKASLRAVKKLKTLDVGDHVSRILSNRKEELQTRILASSVTGLLKLDGLLPYLEEIV
                     VDASSESRLRLSSARAMARINPSRLADLFGFTV"
     gene            complement(27956..28741)
                     /locus_tag="PKL93_04220"
     CDS             complement(27956..28741)
                     /locus_tag="PKL93_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNQ70500.1"
                     /translation="MVEIPLGRFFPADSIIHKLSPVVKTISFVALAVGSLFLSGIVDS
                     LILFSFWISLLLLSKVPLGEYIKTLWTIKFLLLLIVIFQLFFTHGKVLLDMRILRITE
                     EGLLNATLLTVRMVFAVFFSITLSYTTTPMEISSSIEAIVGKLPMKTKRVSELGMALS
                     LTLTFIPLLSLQTNRIIMAQKARGVEFNGGSFFKRVRNSLSVVIPVITTSLKKAQDTA
                     SALEIMGYEPGHTLGSLDKMSWSINDSIILISTAVSITAVVIL"
     gene            complement(28735..29115)
                     /gene="queD"
                     /locus_tag="PKL93_04225"
     CDS             complement(28735..29115)
                     /gene="queD"
                     /locus_tag="PKL93_04225"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNQ70501.1"
                     /translation="MNIEMFFITKEFTFDAAHNLTMYHGKCEKLHGHTYKLQVTVAGE
                     KQEEDMVIDFLKMKEIVRDEVLSVLDHSYINDVLSQPTAENIAEWIFNKLSGKIRGNS
                     FELYEVALWETPTSFVRYRERDGW"
     gene            30152..31228
                     /locus_tag="PKL93_04230"
     CDS             30152..31228
                     /locus_tag="PKL93_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNQ70502.1"
                     /translation="MYSLNIIPGYVELVEILMACGLADRKSYFDAVSHDLGFDFEPGQ
                     ELGAFLLKIRENENWSIKVVADVFSEIKDSVIFFCDFPERPGGEILLEDSIESLANNF
                     EEKSKCVVNSILSNNLNFSQEEVDKILEGDLSIVPEAVNKAEDLSENTTWFITQLINF
                     PRQSKEILLFALNEMKKYYEESGLRKKNRDQIAEKLKLFENDRYNQIAKGFLDFYGIK
                     AREDLPLHLLFQNTLKYSGLKFASSRNIQLIVFSEHFKQIEEIMNPSVTDNTLRLFLR
                     NLADQTKMQILKSISEEPKYVDELAKLVGLSKATVSYHLSTLSELALVKSRKDHRRVY
                     FSLNKERLEKILKGLETLYDEGEN"
     gene            31228..32295
                     /locus_tag="PKL93_04235"
     CDS             31228..32295
                     /locus_tag="PKL93_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNQ70503.1"
                     /translation="MEIITGYFEIYDLTMAIQFSLNTGPVEKVLKTLGVDYEPSSQLL
                     EFRETLLKDEEWSTRMYITFMNELGVMAPILFPIRQKLQDGMQVTIEESLNVTEEGIE
                     LIRDRFIQVFADRRLKISHDELNRMISEEPQKVSKAIEAIGGVSPDTVWFVHQLLFFP
                     KTAMDFLSSKTMKILNYYEGSGLRDLNTRLVKGYIESSSSQKIKDAFSNYLDYYDIVL
                     DETKPVYITLQNSVPHTNSGLMTYPTFHLLIMGLEEVTEDFSGRIPQEVRLRSLLKVL
                     SDETRFKILKRLSKEPALQKDLVEFTGLAKSTISYHMGLLFKSSLIDVDPFSSIICVR
                     KETVRRAAADIRNLLNLRDMK"
     gene            32292..32948
                     /locus_tag="PKL93_04240"
     CDS             32292..32948
                     /locus_tag="PKL93_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNQ70504.1"
                     /translation="MIRAVIFDMDGVIIDSEKLYRRACTELVSELGGKISDELFEKQM
                     GLKMSQTQRVIVQTAGLDMEPEEFGRRYMERYLELARETVVPNIGLIELLDFLYGKVE
                     LAIASSTEKSAVEELMKKINVLEYFSVIVGGDEVRESKPSPMIYQRASRLLGIAPEDC
                     IVIEDSPNGIRSGFMAGMEVLGVRHEENIDLDLSLSKHVFDNLNGVREYLEKRFDSLI
                     "
     gene            complement(32945..33598)
                     /locus_tag="PKL93_04245"
     CDS             complement(32945..33598)
                     /locus_tag="PKL93_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNQ70505.1"
                     /translation="MLDTVDLFNDLSSDEKSEVLSASRTVRFQPDQVIYTPDDLCDSL
                     CIVLKGRLRIAKVFPSGREQTINYIEKNQIFGEALAFAGRKCASHVFAEEDSEVLSIP
                     KKALLYAFKNKRFLFSYLKSISEKTLNLSYIIELLSLSTVKKKLASYLLELSLEKGSN
                     SFELPHTKKTLANLFGSTREAVSRNFSELRKDGIIFMPDRNSVEIKSSKQLEKILFD"
     gene            complement(33599..34336)
                     /locus_tag="PKL93_04250"
     CDS             complement(33599..34336)
                     /locus_tag="PKL93_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ70506.1"
                     /translation="MSRIRRLEEISLNSWPAGYTKFLDGWVLRYAGGYTNRSNSVYPI
                     YDGFEPLEQKIEEASRFYKSKGLRTMFKLTADSKPAGLNSVLKEMGFEERDRALVMTR
                     AIGSEPHDLSDADVASRPEEEWLELFFSLNSRARENQAWARKLLSMLLPGSPFIILRK
                     RGIAVGCGFAVIESNHVGLFGIAVRESERRKGYGREITEKLLELGRQRGAETAYLQVD
                     KPNESAISLYSKIGFREEYQYWYRVGE"
     gene            complement(34565..35119)
                     /locus_tag="PKL93_04255"
     CDS             complement(34565..35119)
                     /locus_tag="PKL93_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNQ70507.1"
                     /translation="MSKTVVLYKTRYGSSKKYAEWIAEELHGDLFDIEDVDPERLKDY
                     DCIVFGGSLYATGISGLSTIKENFKSFENKKVIIFSVGASPARSEVIEDIKAKNFSPE
                     IVEKIEFFHLRGGFDFKRLRLVDKILMILLKSKLKGKKRRGEELSDDERGMLAAYSTA
                     VDFTSRKAIEPILESISKFTHGNA"
     gene            35319..37049
                     /locus_tag="PKL93_04260"
     CDS             35319..37049
                     /locus_tag="PKL93_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70508.1"
                     /translation="MIRKFMAYYRPHLGLFLLDMACAFMIAGIDLVFPRFTTLALDDY
                     IPSGNMRGIVIIAAIMAFLFVLRAVFNYVVSYWGHVVGVRMEYNMRKDIFSHLQTLSF
                     SYFDKVRTGKIMSRIVNDLREITELAHHGPEDVFISTVTLIGAFIILMQNDWRLTLVV
                     FAYIPFMVWYGVRKRQKMAKAFRLVRKKIANVNAQLENSISGIRVAQSFTNEEFEKSK
                     FDLGNQEFKESRQFAFKSMAEMTTGVDLMMNMLKVTVLAFGGYLTYSGEITVGAFVAY
                     FLYVDLFLQPIRRLMQFAQQYEDGMSGFERFVEIMETKSSITDSPDAVELNGVRGDIR
                     VEKVSFAYDGGENVLTDINLHIPAGKMVALVGPSGGGKTTLCHLIPRFYDVKSGRITV
                     DGVDIRDVTIHSLRTHIGIVQQDVFLFAGTIRDNIAYGKGDATDEEIIEAAKRANIHD
                     FIMSLENGYDSYVGERGVMLSGGQKQRISIARVFLKNPPLLILDEATSALDNETELKI
                     QESLEALSKGRTTLVIAHRLSTIKNADEIVVITNNGIIERGSHDELLEKGGHYAGLYR
                     AQFKGYIPDM"
     gene            37402..38478
                     /locus_tag="PKL93_04265"
     CDS             37402..38478
                     /locus_tag="PKL93_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNQ70509.1"
                     /translation="MDIENDFLFRWYERQLLVEGMTRSFLLRLAEETFGHLWGATEEL
                     TAELLIRSGLSGITRDFPRHRILPFYSPLIYRQKETMEFREGDDFKIEEDCGKRIVSF
                     PHTLSIMFSPLIAGKVFSWLMNGSNEIGPICDLPLDLQRASRKGRVMVVGAGGLGCPL
                     IKILLDNGIDDICIIEPGEIKLNNLHRQILYDYGDVGLSKASVIEKKIAQRYSGVRVK
                     IRKESFDPTLIKTEKPDVVVSCVDNYEARYLINDSCYRNCVPFVDSAVENFGGYAMFR
                     DKRVPCYRCFMGDNRKDTGAQKGILTFVSYFGGMLEAAMVLTFLNTGMCGDDVFWFDL
                     RKGKFESISFERREGCPVCSSAKR"
     gene            38485..38685
                     /locus_tag="PKL93_04270"
     CDS             38485..38685
                     /locus_tag="PKL93_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70510.1"
                     /translation="MEVVYGNNRWSFDRDLTFSELIEETQLGSVSPLVLVDGRFVGED
                     ELISAKSRVLIIVAANADQTGA"
     gene            38828..>40127
                     /locus_tag="PKL93_04275"
     CDS             38828..>40127
                     /locus_tag="PKL93_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNQ70511.1"
                     /translation="MERKKVLILGAAGRDFHNFNTYYRDNEEFEVVAFTATQIPGIDG
                     KKYPAELAGRLYPNGIPIVPEEEFGKLIDEHKIDQVVLAYSDLPHQYVMERAAIANAH
                     GADFILLGPSKTMVKSSKPVISICAVRTGCGKSQTTRRVLDILRAKGKKVVSIRHPMP
                     YGDLVKQKVQRFADYSDLDKHECTIEEREEYEPHIDRNAVIYAGVDYEAILREAEKED
                     VDVIIWDGGNNDFSFYKTDLYITVTDPHRAGHETTYYPGFTNLMMADVVVINKEETAS
                     PEDIDIVRRNIAKFNPDAIVVDGASPITIEGGKSSEIKGKRVLVVEDGPTLTHGGMKY
                     GAGWVAAKKFGASEIVDPRPYAVGSLVSTYEKYNHLDQILPAMGYGEKQMKELEETIN
                     KADVDLVIIGTPIDLRRVIDIKKPAVRIGYELQEIGKPDLE"
     assembly_gap    40128..40227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(40228..40437)
                     /locus_tag="PKL93_04280"
                     /pseudo
     CDS             complement(40228..40437)
                     /locus_tag="PKL93_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(40722..41057)
                     /locus_tag="PKL93_04285"
     CDS             complement(40722..41057)
                     /locus_tag="PKL93_04285"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNQ70512.1"
                     /translation="MCHLSNDRSALSPELRESLAARIGTEMEKILEYLKLSDLSRLGG
                     MKGVRVRLYCNAGLDTLDKLSNWNPEELWAMLVDFVRKTGFEGIPPLPKEVSSTIEAA
                     KKLERLIGY"
     gene            complement(41519..42448)
                     /locus_tag="PKL93_04290"
     CDS             complement(41519..42448)
                     /locus_tag="PKL93_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HNQ70513.1"
                     /translation="MVIFSLTLNAALDRFLYTDALIEDDTVRVDRVKDYPAGKGVDVS
                     RVINELGGHSVAVAFLGGHTGKVIEEMLDDEGVVYATVRSEEETRTNILVQTGSGQYR
                     MSLPGPVISVKEVYKLYKTIDVLLRKDDYLLLCGSIPEGVSSDIYAQLCSRMRKIGVN
                     VYIDSDKEPFSKGVEAKPRGIKPNIHELQRLLNRELIGEIDFRKAISEVSNKYSIEEI
                     LLTMGAEGAIASVSGEFYRIRVPKVPVKSGVGAGDSFLAAYCMYRELGETVKTALKMA
                     GAASSAAVMTPGTELCRFDDVMSLASKITVEAL"
     gene            complement(42458..43144)
                     /locus_tag="PKL93_04295"
     CDS             complement(42458..43144)
                     /locus_tag="PKL93_04295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNQ70514.1"
                     /translation="MPNAFFVLPEDNRVILDGGETKHLRVTRAKPGDELTGIDGRGTV
                     YRFLLEELGKSSASGTVVEREYVERDGKSITVAVAATKWPRLHILIEKATELGVDRIE
                     LFNSLRSVSRVDETKLTKFNAVAREAAKQSVNPYIPEITVSQTLPLEGSYNLLLEFGG
                     RPLLEVEEKLACERKLRLIVGPEGGFAPEEIKDISTRCTPVSLGRRTLRVETSVIVVL
                     GIINYRLGRM"
     gene            complement(43145..44413)
                     /gene="serS"
                     /locus_tag="PKL93_04300"
     CDS             complement(43145..44413)
                     /gene="serS"
                     /locus_tag="PKL93_04300"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNQ70515.1"
                     /translation="MIDVRLIRENPEIVRRALEYRQMTSELLDEIVYLESKRREALQV
                     VEQKKAERNSISSEIARAKASKDESLSLSLMERAKEISSEVKELDLQTSQIEEELKKK
                     LLYLPNIPSSTTPVGKSEEDNVVIRMWGEPRKVDFEIRPHWDYGPETGLIDFERAAKI
                     SGARFTILRRDLARLERAIANFMLDLHHSKGYEEVALPFMVKRDTMQATGQLPKFEEE
                     AYRIDPDDMFMIPTAEVPLVSQHMDEILEGDLPKKYTAYSACFRREAGSYGKDVRGMI
                     RVHQFDKVELVWFTHPDDSYDALENLTADAEDVLRKLELPYRVVSLCTGDIGFAAAKT
                     YDLEVWLPSYNTYREISSCSNVEDFQPRRANIRFRDVDNKLKFVHCLNGSGLAVGRTL
                     VAIVENYQRRDGKIDVPKALVPYMRQEVIG"
     gene            complement(44429..45094)
                     /locus_tag="PKL93_04305"
     CDS             complement(44429..45094)
                     /locus_tag="PKL93_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA family protein"
                     /protein_id="HNQ70516.1"
                     /translation="MKRRTTLIIGLIVIFLTATVFASTVRIKGDTVLGSLQKEVFTFI
                     GNVFINKDGDIYVETPLATATKGATDWESFVTEGETFVRFQTGEATALSLDYNLENST
                     GTLFGNVEAVIYSKEEDGKDIFIYETEILNFDQKTEYYEGFAEETVEATPELIFINYK
                     GELNVDTLYFEYFGDTGLLNLKGNVFVDDFKNSRKIWASDLIYDTTDDSFEGKNVEIE
                     LVF"
     gene            complement(45111..45569)
                     /locus_tag="PKL93_04310"
     CDS             complement(45111..45569)
                     /locus_tag="PKL93_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNQ70517.1"
                     /translation="MSVGEVKKEDAAILEKNLRTISTRIRREGRKVLRDFPITPAQFD
                     VLQVLFFNGEKRMSDISRWLGITKSTTTGLVKRLIDADLVERRRSDKDRRSYIIDISA
                     AGKTLIEKVIDRRVEYLKSVMTEIKSDQVKELEVIIKNLLEIMDSKKTKD"
     gene            45747..46451
                     /locus_tag="PKL93_04315"
     CDS             45747..46451
                     /locus_tag="PKL93_04315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNQ70518.1"
                     /translation="MKSAKRDLSELVRFDASYRADYRVIAGVDEAGRGPLAGPVVAAA
                     VIVLNPVDGVYDSKALCRKTRELLFGQILENSIVGIGLSSPEEVDILNVFAATRLAMN
                     RALRILSRKPDYVIVDGKSLKLDIKGECIVGGDRRSAAIASASIVAKVFRDRIMDSLD
                     ILYPEYGYRNHKGYCTDMHLRALRKHGPTTWHRLTFRPVRELITKERASRWSNESEVS
                     QIRLFRAGLATMEADK"
     gene            46448..47233
                     /gene="mazG"
                     /locus_tag="PKL93_04320"
     CDS             46448..47233
                     /gene="mazG"
                     /locus_tag="PKL93_04320"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNQ70519.1"
                     /translation="MKNEFDGSEWLRLIETMKILRSPGGCEWDIEQTHQSLKPYLIEE
                     AYEVLDAIDGGDDKELAEELGDVLLQVVFHAQIAMERKAFTITDVLKVLTDKLIRRHP
                     HVFSDSEGYSYRQWEEIKAKEKAKDSGKSSSIGEINKALPALSLARRVQENASVVGFD
                     WGEIEGPREKLNEEIEELDSAIVEKDKKKIEEEIGDVLFTVTNLSRFLDVDPEAALRK
                     STEKFLNRFHEMESHIEKHGLDINNMTIDELNHLWEIAKEGTN"
     gene            47230..48249
                     /locus_tag="PKL93_04325"
     CDS             47230..48249
                     /locus_tag="PKL93_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNQ70520.1"
                     /translation="MKKFIAALILLLITAVALSQTETLSETGIAAEVNGEIITMDTFL
                     SKVLPNYTEISKRIEEVDPLFSEMLLNTEAGQKLLEEYERNSLEALIEETLLIQYAKE
                     VGIEADIEALREVVSKSIMDTLTELQIEKSDADLFYILKGYIDGLSSYEAKVVRDLAY
                     KEVLSSLYEAVTVNATVTEEEIEQYYLANSSRYAVEEERADMKVLLFDSFSEAYSVWK
                     AASRDANPSSVLDTFPEVQSGSYTREEIEALNPELVKSLFKPTEGELLSSVVTLDNKY
                     AVIYLESYSPAGAKGIEDVREEIQGILISEKKDLFWRIWQEQEFKPFKEEAVIKRYYE
                     TGGGE"
     gene            48257..48553
                     /locus_tag="PKL93_04330"
     CDS             48257..48553
                     /locus_tag="PKL93_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNQ70521.1"
                     /translation="MTKEDVMKALGEVYDLEIGFDVVSLGLIYGVELENENDVKVKMT
                     LTTPMCPLAGLMLEDARRKVSEIEGIGDVKMELTFDPPWSPEMASDDVRKILGM"
     gene            48550..49383
                     /gene="prmC"
                     /locus_tag="PKL93_04335"
     CDS             48550..49383
                     /gene="prmC"
                     /locus_tag="PKL93_04335"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNQ70522.1"
                     /translation="MNLQELIHISKRKLSEAGIKNSRFVMISLFRGVLSLNEADLILN
                     GEKEIESKLSAKYMKAVERVVEGEPIDYVLGFREFFGIRLGVTRSVLIPRNETEELVE
                     IVIDEEKNSKVFADIGTGSGAIACALARNIPSSLVFATDISRDALMLAEKNARTNGIF
                     NIRFIEGDNISGLNDFLDSVEVIVSNPPYVRTGDLDSLDTNVRKYEPIVALDGGKDGL
                     DFYREFLKYLPTGKKVYLEISQYETEGLRELVSDLNGYSCEFKKDLSGNYRFMILRPE
                     V"
     gene            complement(49358..50113)
                     /locus_tag="PKL93_04340"
     CDS             complement(49358..50113)
                     /locus_tag="PKL93_04340"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HNQ70523.1"
                     /translation="MSRVSQEARNFISLIRESTCTSVLTGAGISVASGIPDFRSPGGL
                     YSKISPDIFELSSFIEDPARYYRVAKERIHTMSDVSPNATHILLTRLQTLGLIETIIT
                     QNIDGLQQKSGAEEVVELHGTVSKFECMQCKKRFTRKEVELLLERSDVPRCDCGGLIK
                     PNIVFFGEMLPQDAIRRAENAALKSDLFIAMGSSLMVYPAAQFPVIAKSSGARVAIVN
                     RDETGLDYLADYIFSVELESFSEEVLRLLDEES"
     gene            complement(50106..50699)
                     /locus_tag="PKL93_04345"
     CDS             complement(50106..50699)
                     /locus_tag="PKL93_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4897 domain-containing protein"
                     /protein_id="HNQ70524.1"
                     /translation="MKFNTLLIILVVVMVGMTAINMFMAFNNRLDIDTLSSTSNYSYD
                     YEGRATLDIETEIIFNKPDQMTQFLEQYDKPQEEQLADFQESMTQFSESFDRLMLVES
                     FQSTATVLSSNRVKVVEHAVIAGFAAVENGVVNTDMGDMEFDLTGDAYSLTISIPADA
                     KVINLDPAPTVAADGNEFIWTNTGKTIFPKIQFSRGE"
     gene            50843..51826
                     /gene="trpS"
                     /locus_tag="PKL93_04350"
     CDS             50843..51826
                     /gene="trpS"
                     /locus_tag="PKL93_04350"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNQ70525.1"
                     /translation="MRILSGMRSTGRLHLGHFITLEKWKELQDQGNECFYFVANWHGL
                     TSHIDETSSFYDWSLDIVRTYVSAGLDPEKSVLFIQSAIKEHAELFLYFSMLVSVSRL
                     ERVPTYKDQKEQITDRDLSSGGFLLYPVLQAADILMYLATGVPVGEDQIYHVELTREI
                     ARKFNNQFAEVFPEPEPILAKVPKLPGTDGRKMSKSYGNYILIDTDEKELWKMIAPMM
                     TDPARMRRTDPGDPEKCPVWDYHKAFTTSDEEKRWVVEGCKTAGIGCIECKRLLHKNL
                     VQRLSPVWEKLSHLKENSDELFEIIEDGNRKARVVAKETMKRVIEATKLGW"
     gene            51829..52521
                     /locus_tag="PKL93_04355"
     CDS             51829..52521
                     /locus_tag="PKL93_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNQ70526.1"
                     /translation="MEQLELVFSFPEFEGPLDLLVFLVRKHRVDIRSIPITVIADEFI
                     THVNKMKSLDMVVTSDFLVMASTLMEMKSKALLPRMSGGEAETFDNQRKELYRRVEEY
                     KALKDLSKEFRVKLYDAYSYERATAKPTVDQKRKEDLPEELTEAFKAILKETVLRDRV
                     YTIVSESINVEDRMLQIEQLYETIELYKFLMSLESRSEIIVSFLAVLELLKLNRYYLD
                     TVEPLTLRRKAS"
     gene            52518..53156
                     /gene="scpB"
                     /locus_tag="PKL93_04360"
     CDS             52518..53156
                     /gene="scpB"
                     /locus_tag="PKL93_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNQ70527.1"
                     /translation="MSEELTKKAIIEALIFSARSGIEPSRIASITGMKLNQVMLLLEE
                     LEREYGGHEHGVMIKSVNGKLRFYTKASIQSFVSQISVRPLVSITDTQMESLAIVAVK
                     GPLTRNDVELIRGRSSQGQLLELSKMGLISKRKSKLPGRPYLYKVTSRFYELFQVDDL
                     KEIVNGLAFSKEEDDLETSRDNLSDNEHVKEKVDSSDSGRESESERPDRDLP"
     gene            53044..53814
                     /locus_tag="PKL93_04365"
     CDS             53044..53814
                     /locus_tag="PKL93_04365"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNQ70528.1"
                     /translation="MKLHEIISQITNMSRRKSTQAILDGRVKVNGRIETYPRLDVFPE
                     SSEILLDGSRLIFSNEEKVVYLVNKPVGYLSAMSDDRGRKTLSDLVSERIKERVFHVG
                     RLDQDSCGLILMTNDGDLSNLVSHPASEIEKTYVVGIKGKLTDRDLEAVKGGLTLGDG
                     FMTSPAKIRLVRSDGDFSKYAITIHEGHKREIREIFRVLNKSVSSLVRVSIGPLSISL
                     VPNPGDIKRLSRKEIASLENGAHRKAPKKAQRKNNFGS"
     gene            complement(53824..54774)
                     /locus_tag="PKL93_04370"
     CDS             complement(53824..54774)
                     /locus_tag="PKL93_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HNQ70529.1"
                     /translation="MKRSLVIVLLLSCVIGVAVSIDFESLYTMERASDFPLLMPQGIE
                     WESKAVFNPAAIMIDSTIYLLYRSEDWSGTGRWNGTSRIGLAKSTDGFEFTREPSPLI
                     YPTEPYEIPGGCEDPRIVKIDDLYILTYTGYDGGKARLCIATSKDFAEWEKLGPVFKD
                     DTWSKSGAIVPAKIDNKYYMYFGDSSIKLAYSTDLKNWTIFPRPVMEPRQGSFDSRLI
                     EPGPTPIITDEGILLIYNSADFSTIYRPGAALFDIENPRKIIKRTDKPLAEPELSWEK
                     QGQVPNVIFIEGAVIKEDRLILYYGAADTYIGAFVVDLTD"
     gene            complement(54779..55669)
                     /locus_tag="PKL93_04375"
     CDS             complement(54779..55669)
                     /locus_tag="PKL93_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNQ70530.1"
                     /translation="MRVVIKLERHPLNPLFSPISQHHWESKYVFNCAVIKRQGLFHMI
                     YRAQGEDMVSRMGYAVSLDGVRFNRFEKPVFTPGSQLELYGVEDPRLTEIEGKVYMQY
                     TAYSPKGVRISMASTTDFLRWERHGVIIPDVDNKDAALFPAKVKGRYLMFHRIEPDMY
                     LAYSEDLRTWTEFTPIAGPRSGKWDNLKIGVGAPPIETEYGWLVLYHGVENTPRPTYR
                     LGFMLLDLENPEKVVKRSDEPILEPEEEWEIFGGVPNVVFSDAMVEHEEKYFVYYGAA
                     DNHIALATIDKETVVKWIRS"
     gene            complement(55673..56491)
                     /locus_tag="PKL93_04380"
     CDS             complement(55673..56491)
                     /locus_tag="PKL93_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70531.1"
                     /translation="MKKVPTIILYVFLFAASILWIYPYVWMLLSSFKPTNEIYSGFLP
                     SQFTLDNYRFILEAADKMERPFIQALGNSIFISVTVTLGVLVSSALVGFALSRLNFKG
                     KNAIFNFIIFQMIFPGFLFIVPLYVLIKNLGLIDTRTALILPSLVSAWGVFMFTQSFR
                     AVPNEYLEAARMDGANDLWIITKVLVPLTSSTVSIVGLFTFIGIWDNFMWPLIVVSDY
                     SKMPLSVLLASFNMQYGSYLGPVLAGSVLQTMPMVLIFLVFRKYFLQGISMSLK"
     gene            complement(56488..57366)
                     /locus_tag="PKL93_04385"
     CDS             complement(56488..57366)
                     /locus_tag="PKL93_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70532.1"
                     /translation="MRKRLKTIDAIKGWSLSGGYLVYTAIFWGYPFVWLVILALSKWN
                     YFTPRKFIWFDNFIELFQDEMFWRVFFNTFNFMLYFIPMVLGLSLLFALGLKRVKLFR
                     TFFILAFLVANVSSGVAYSIIFQKLFAINGPLNGLTRAIFGITIPWFSSPQLATLSIA
                     IMVTWKFIGYYGLILFSGLQAIPESLYEAAELDGAGKMTKFFRITLPLINPSIVMVLV
                     LSLTLTFGIFTEPFLITGGGPMRTTYTFQMLIYTTAFQKINPGYASTLAIVVALLSYG
                     CVLLTRKLVEREVEIV"
     gene            complement(57385..58635)
                     /locus_tag="PKL93_04390"
     CDS             complement(57385..58635)
                     /locus_tag="PKL93_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ70533.1"
                     /translation="MKKLILLTLFSCLFVGLFAVELVFWTAPNPLQESFWKEVVAEWN
                     ENNPDIQIKWSTIPAAGSSEEAILTSIASGRMPDICTNIFSGFAAQLIEADILIPLNS
                     FPDFWELVEQRKMTSIVENWKFGDNYYVLPIYSNPIMMWWRSDILKELGYDRPPRTYS
                     EIYEISEKYSVPYEKYGALVVMGRNWYDRWYDFITYYYAASEGAPYLDVEKARALFNN
                     ETGEKVVTFIDTMFRNNWTAVDLGSNPFYFGVVLGGIFGPWEINQARNLFPDVFDHIT
                     IVPPPVPDDYPEDKPIYTFADTKGLVMFATTKHREAAWKFIKWVYSDIEKDRKWMEFT
                     NLPPAREDLLTNPIFQEYMEDNPKFAAYASHVAYAVPPALTTKTVEVQEILTTFLIEQ
                     IMYGKSTPLDALKDSATRVRRELF"
     gene            complement(58653..59630)
                     /locus_tag="PKL93_04395"
     CDS             complement(58653..59630)
                     /locus_tag="PKL93_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ70534.1"
                     /translation="MSAKLSDVAKLAEVSTATVSRVLNKSGYVSKKSREKVMLAVEEL
                     EYKPSKVASFLASRKLAFNIGIVAGKRLFRVLSDQSDQFYTIVLKGIEEFFAGNNMRG
                     TLVPSNDFSEDYDGYLLIGGEITEEDIRRAKETKKPVVLIDQYLAGVKVDCVVSDGYD
                     GAIYGMKKLISKGLKKIVNIHGPLSHFGFKDRFDGYISAMEGAGYLPKAYEYDEDNDN
                     MSAIIDLLLSRYGLPDAVFGCNDTAAIRAMEELQARGINIPEEVSIMGFDDIVNSSAT
                     TPSLTTFKIFKHEMGVVGSRRLQSLLIGNEPHPTKISLFTEFVQRESTL"
     assembly_gap    59670..59769
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            59815..61140
                     /locus_tag="PKL93_04400"
     CDS             59815..61140
                     /locus_tag="PKL93_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70535.1"
                     /translation="MKRFLVLFFIVFSVFSVAETVRVPVSVSSFTGEPIAVTISMSEI
                     LDLVGVDFDANWDSLKVVQDGKELPYQIDDTDLNGKLSSGDVMAFLITGPAEITVTDD
                     FDILPPEFVSVGKVVEEEGQWLIEIGEITAVANSKGLVKVTGFGDVEGTIVDELGIVR
                     MSGYVGSTYYVDGEYGRHEEKTSGDFIVKEATVLPAGPVGITVVSTLEAQPFLGITQK
                     IITTLFSNGDIISHNTFEFATYAELMKLQIMATRVLTDAAEDTVHTLPVFRRLLWADQ
                     LNITPLEYWLDRNAIMFSGNKPYIVFPAVDSMRPLWWGATYIFASQESWRANYSQSLK
                     TGVAEINPEVPVVVADYEEWMGGLTWVYESREFRDGYFEWMPGEIDIFESTKDYVSSN
                     WEDYVKHFVAGDVVSFKRVYSIYSADSIEDSIEFVEMKKSEIQSLKIGE"
     gene            61198..63513
                     /locus_tag="PKL93_04405"
     CDS             61198..63513
                     /locus_tag="PKL93_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70536.1"
                     /translation="MKKALIILVLLISLMLLGIEVQINLDHLEFLRDHFAVEGESEIG
                     YWIYADRLPDGNYRHADAPGEGVTCVDDVARAAVLYLRLFEISGMEQHYDRAKEALEF
                     VLSMQDSDGDFYNFVFEDGSINRNGPTSRKGGNWWAARALYALAKGARVSQNLDPEFS
                     MRLADAAQRCFRKIIGFERDGLIQGYTDLSSVVLLGAAELYSMSNDALLGGFIKRCAA
                     ALQSKVIQNEDSIFNGLVDESSPSEPRYFWHGWGSRQLEALAVAGEILGDDSLLEIST
                     ESFKQSSLLLINAGPLYSISSYLQLFPQIAYAAESAISAGFRLYHLTGDRDIAAITAL
                     LGSWFLGVNKLNQSMVGKNGEGFDGLEFSHVNKNAGAESTISMLLSLERIKRLPEEYG
                     DLFSAAIEVLTPVVVLEAESMRAGLSESEIIQSTGVSGSAVTKISGAFSLRQPVTLVE
                     ASYSVFAAINEAKTNEVVFTLRMGESKSEKTIRIEEKTVQKLGVLKGNGKETTLTVGG
                     KVSEGSFDIDQIILVPDIVAVYLVEDDVTIVFNFKGCSEFDEGVSVIDGRVFEREKSV
                     LQVPVLPVSLIEMNGFVHLDLLKYFDNNGIATSDERKTANFDNPQGQFGASYISSELE
                     KAIDSGFLAVEDTEFMVNVDGNDNFRLTGQALTFSAEMFDKICILGAANHGDYTGEAL
                     LIYEDDYQQVIPLSFSDWCGGPSTGEKIAFEFSGRYDNTGNTERIKCMLYYCCINLLE
                     KPLKGIVLPQMPNVHVFAISLSRAEPGSQEE"
BASE COUNT        17426 a        13975 c        14683 g        17561 t
ORIGIN      
        1 agtccttctc aatgttgcgc tctttcctgt tttcatcttc ggatgtggaa tgagtatcgt
       61 gggagcggcc gttgcgtcag tcatctctca gtctgtttcg acgatgatca ttctcgaata
      121 ctacataaga aagaagagta gcacgaatct atctataaga ctgtacagtc tctctctctt
      181 cagatatact ctgaaatctt caaaatccgt ctttcgacac tcctgaggca atctctgtca
      241 agtttcgcac cgcacttctg aatcacgctg ccggaaacta cgaggatcat gtcgtttcgg
      301 ccgtcgaaac aacaatgggg gttctcatgt tggggatgat ggttctattc ggatacgatc
      361 ttgcatttca accggttgct ggctataatt aaggcgcgaa gaagtcttct agggtttttg
      421 aaactctgag atttttgctg atagtgatta cggtctttgc agtggttttt gccattatcg
      481 ggttgactat accgagttcg attataagca tctcctgtaa cgacccagaa atcatcgatg
      541 cgggctccag agcctttagg gtggtaaaca tcttcttttc cacattcggc ttcgtgataa
      601 cttctacgga cctgcttcag gtttttggaa agggaatcgc agcgagattg ctgccgatgt
      661 cgaaacaaga tttcttcctt atacctgcga ttatcttttt gcaaaagatc ttcgagctca
      721 atgaggatca tttttctcgg acagttgcca gttactttgc gctatttgtt gcagcttttc
      781 tttccgtagc aattttcaaa aaactgaagt ctcaaagcag ggagctttct ctacgagaaa
      841 ggcagccagt gattattaga atatcaccgg tgcatgacca tcttttatga acgcccatga
      901 catatccttt gaagtgtgcc ctattccgat attgccgtat ctgtcgagcg caattaaacc
      961 gcactcacag tcgaagtatc tggctctctc gagagcgatg tcgacggact tttgagcggg
     1021 gtactgttcc atgaagaagg caaccagcct gctcagagtg agcttcataa tcttttctcc
     1081 atgacccgtt gcggagactg cgccgaatga atccgagtag gttcccgcgc ctataatggg
     1141 gctgtctccc accctgccga agcttttcat catcattccg cccgtggagg ttccggctgc
     1201 cattcttccg tggtcatcta tcgcgactgc accacaggta gaatgatact cctcggcctt
     1261 tttcgccagc ttgacgctct ttccaaatct ggtcttctcg cctctggcaa ttttctcaag
     1321 ttcttctttc cactgtaggt gccttttcgg aacgatcagg ttatcctccc tcggaaaccc
     1381 tagaaccgta acgaattcct ccagttcagc cccggcaagt aaaacatgat cagtttgttc
     1441 catgacggcc cgtgcaaccg ttatgggatg gagaatcctc ttcaaaccgg aaacagctcc
     1501 cgcatttaaa ttattgtcca ttatcgcagc atccatttcg atatcgccgc gataagtaag
     1561 caccgatccc ctgccacagt tgaaggtcgg gtcctcttcc atcacggaca cggcggtaac
     1621 aacggcttct tctgcgctgt ttatcttctc gagagttaga aatccttgcc tgactgcctc
     1681 ttcgattccc cttctgtgtt catcggcgtc agttacgctc ttgaaacttc cggtaccgcc
     1741 gtgcacaatg atggccttca tacaaacacc tcgtttcaag caaaagaatc ttccttgatt
     1801 aaaactagaa cactcctatc taaaaaagaa ctcagatgcc acagcgggct aaatcaattc
     1861 ccctcttcga ttcccttaat cttcttttca gtaattagat gcattcacga gcaaacgatg
     1921 aatctacttg ttcttgccgt caatctttat tgctttgtat cctctctcct ctacctcaaa
     1981 cagccttttc atctcctcca taccggtctt cttttcgctt aggatgatga agttacctat
     2041 catttccgcg accgctatgg gaccaacgaa ggagtttccg aagagaagtg agttgttctc
     2101 acacagaatc gacgaatagg ctttcgagta gaccggacag tcaacggaat ctgtaacgag
     2161 aagagtcctc acatttctct ccagtgcaaa gtcgaccatg tattccactt cttttgaata
     2221 tggaggaaag gtcatcacta tcaaagcgtc acttctttcc atgagcttta ttttgaagga
     2281 aaagtcggag accgatccag aaagcagcca ggttggcttc cgctgaagaa cggagattgt
     2341 gtacctcatc agcgaaatca cgtgttttgt agcgccaaag cccccaaaat agatgttact
     2401 ggctgctcta atagtgtcga cccatttgtg aagttcctct tctctgatcc cgtataaagt
     2461 attcttaagg ttgttgatct cattctgtct gagcaatttc agctggtttt ccaccggggc
     2521 cctgtcaagt tctgtcgtcg cgatcttctc atagggtaga agccttctcc ttagttcatg
     2581 ctggatagtc ttcttcagtt cattgaatcc cgagaaaccg agacgtctcg agaatcggac
     2641 tacagaagcc ttgctcgcac cggccattga gctcagttct tcaatagaca tgaaccctac
     2701 ctgagagtat tctttgagaa tgaaggttgc tatccttttg tatgcaggcg ataagtcatc
     2761 tttgattttc gttatctctt cggataccat tcccatcaac ctttcggcaa gaaattagtt
     2821 aattatctga ctcatgaaac tatcttttca taatgatagc atattgaaac tatgttttca
     2881 taacagcata tacttcttct cgctagaaac aaagttgata actgaactat ggaagcttgg
     2941 atcttgatcc tccactttga gagattcagt ctgattgata tgatatgttg aagatccatt
     3001 ggagtagttt ggagccgtaa tgcatcataa ttaaatctac tcaagaggga gttattccac
     3061 gtaacagcgc agatgataga atatagttac attaattagg aggtgtttgt atgaagcgag
     3121 ttcttatctc tgtgatcttt ttggtgttct ttgcatcagc ctttgcaaat ctcgtggtct
     3181 attccagcgt cgatgaggca aatgcgagga agatattgaa tgcatttgtt gaggacacgg
     3241 ggatcgaagt tgactttgtc ttcctttctt cgggacctgc cctggcaagg attgaagccg
     3301 aatccagcaa tccgcaggcc gatgtgtggt ttggcgcacc actggaaaac catattatcg
     3361 caaaagaacg tgggttgaca caggcctaca agacgttaag tgtatacggt gtctcacccg
     3421 agttctatga cgttgagggt tattaccatc cgatttatat gaatcctctc ggtgttggag
     3481 ttaatacctc cgtcctggag cagataaagg cctctttgcc ggaatcctgg gaagatctcc
     3541 tcgcttccga atacagaaga atgattcagt atccaaatcc ccagtcttct ggaactgcct
     3601 acagcttgat cactggttta gtaaagattt acggtgaaga tggggccttc cagtacctga
     3661 agaatctagc tccaaatatc cagacctata cacagagcgg aactggaccc tccaaggccg
     3721 tcggccctgg tcaggttgcg cttggaattc agttcacccc ggctttcttc cagttcatgg
     3781 aacagggata ccctgtatcc gttgtgttcc ccaaggaagg agtgccctac gaagtggctt
     3841 cagtctcgat tctgaaggga gccaagaacc ttgcggatgc tcagaagctt atcgattggg
     3901 tcgtttccaa ggccggtcag cagcagatag ttgaccagaa gacatacttc taccccataa
     3961 gacccgatgt cgatttcggc agcctacagc cgctttcaac gatcaatctt ctttccatcg
     4021 atcctgcttg ggccgcagaa aacaagacca gacttatcga gagatggatt aacgaagtac
     4081 ttccatacta acgatcactt agatttgctt tgagggggag aaatccccct ctttaatagg
     4141 aggtcgtcct gtggctataa ggggagaaga agcaaaaaat aggtttctta atcttcttaa
     4201 ggatccagtt ctgcttttga taatagttct gattttcgcc gccttggcca tattcattgt
     4261 ctatccccta ttcagggttt ttgcggttag tatgaccgac aaggaaggcg ggtttgacct
     4321 gagcgcgtac agtcatgcct tttcgaatcg atacatgagg cagggctttt tcaacagtct
     4381 tctggtggcc ggccttacgg caatattcgg aatgcttgta gggtatctat ttgcgtacac
     4441 gcttaacaga acggatattc cgctgaaggg ctttttcaga accgttgccg ttctgccgat
     4501 agtcttcccg ccgtttattg gcgcactttc gatcataatg ctattcgggt tcaacgggat
     4561 cattaccgcc gggatattcg ggataagaaa ttttcctgtg tacggtttat ggggtctcat
     4621 gatggcccag gtggtttgct tttttccggt tgcgttcatc acactggatg gtgttatagg
     4681 aactatcagt ccgacgcttg aggatgctgc cttcaatctc ggtgcaaatc ggtggcaggc
     4741 cttcaggaaa gtaattcttc cgatgtcggt tccgggtatt gcaagcacaa tgcttgttct
     4801 cttcatagag tcgctcgccg actttggaaa tccacttatt ctggctggca gcaaatttcc
     4861 tgtgctttcc gtccaggctt atcttcaaat aacgggaatg tttgatacta aagggggagc
     4921 agcgcttgca gtatggctac tgtttccttc gatagccgca tttattatac agaaatactg
     4981 ggttggaaag aagaagtatg tcacggtgac cggtaaaccg acgacttctg tccttaagag
     5041 tgtaagtaaa ccggtaaaat ggattctctt ttcactgtgt atgcttatcg ctgtcttcac
     5101 actcacaatc tatgccacaa ttttctgggg agcctttgtg aaggcgtggg gaatgaacaa
     5161 tactcttact ctggagaact tcaagtatgt cttcgacgtc ggactcgagg caataaagga
     5221 cactctcgtt attgctttca cttctacgcc gatatctgcc gtccttggta tgataattgc
     5281 ttttctcctt gtcaggaagg tctttcctgg aagaagggtg atggagttca cctcgatgct
     5341 gagttttgca gttcctggaa cggttattgg aataggatat attctttcat tcaacaaacc
     5401 cccgttgctg ctcacgggaa ctctggcgat acttgttctc aacttcgttt tcaggtatat
     5461 accggttgga atacagtcgg gtgttgcttt gctaaaccag gtagatccag cgattgaaga
     5521 ggcggcatac acgcttggcg cagataacag gcaggttttc acgaaagtca cgcttccctt
     5581 gattatgccg gcctttttct ctgctttagt ctttgccttt gtgagagcta tgactgcaat
     5641 cagcgcggcg atatttcttg tttcggccag atggaacctg ataacagttc aaatactgag
     5701 tcagagcgat tccggacgtc tatcggaagc atgtgccttt tcagtgctat tgattggtat
     5761 gattatgggc ttcattctga ttctgaagat cttccttaag aacaaaatta gtctatccag
     5821 tggtggaacg gcaggacagg ggtgatttga tgagtctaat attaaaaaat gtgagaaagg
     5881 tcttcacgag ctacggaacg gaaacggttg ccgtagacga tttcgatcag gagatccgaa
     5941 gagggcaact ggtaacgctt ctggggcctt cgggttgtgg aaagactacc acattgagga
     6001 tcatcgcggg ttttgaagtt cctacaaacg ggcgcgtctt gctggatgga aaggacgtca
     6061 caaacctacc tcccaaccgg agaaacattt cgatggtctt ccagagttac gctctcttcc
     6121 cccatctaac agtggaagaa aatatctctt ttggtttgaa gctcaagaga ctggacagaa
     6181 gcaccatgaa gaagaagata agggaaatga cagatctcgt aggactcaag ggacttgaaa
     6241 agagaagacc ggatcagttg tcgggaggtc agcaacaaag ggtggcacta gcaagaagtc
     6301 ttgttatgga accaagtgtt ctgctcttcg atgagccgct ttcgaatctg gatgcgaaac
     6361 tgagagagtc tatgaggctt gagatccggc gaattcaaca ggaggtaaat attaccagtg
     6421 tatatgttac tcacgaccag gtcgaggcga tgagtatttc cgacataatt gttgtaatga
     6481 acgatgggaa ggttatgcag atagggagtc cgttcgacat ttatgcgaga cctcagaaca
     6541 gcttcgtagc ggatttcata ggaagagtta acttcattga cggcgaggta gaatctatca
     6601 atggagactc ggtaactgtt tcatccagtt cactgggaaa aaagtttgtg ggcagaaaag
     6661 gtggagagct ctcccctgga gacggtgtaa ggatagtctt aagaccggag tctttatctg
     6721 acagagaaga agacaagatc aatgttctaa gtggaaaggt gttgaaatac gtgtttctgg
     6781 gatcgaacgt cgagtacgaa attgagcttc ccgatggaaa gatactaaac gcagtgactt
     6841 tcaaccctat tgagaggaag tttccagtcg taggaaagga aacggatctg tatttctcga
     6901 aggagagcgc ttgggttata aacaactgag atggatggcc acgatgtctt gactaacaaa
     6961 tgcatggata tgaaaaaagc ctttctatct gaggaagtcg agattaaacc gtactgctat
     7021 cgggcttatg atcagggtcc gtacaatagc tctaaggatg gttttgaaga ttaccttgga
     7081 aggccgtacc tttctcgctc agatgaagag ctgtatttac cctttttcga caataaggcc
     7141 cccagaagcg gcagagctcc cgcatggcag attctgacag tttattcata tgaaccagac
     7201 tttggcatgg atgaaggctt aagtctttca cctttacaga aattcatggg aggaagtgga
     7261 acgtggcgtc atgaggagtt tcatattatt ctccgattcg gcgaagtgac agacagattt
     7321 ctacacttcg accgtatgag catgctggcc aatgaaaagg gagataggta ttgggctcta
     7381 agatttgcag ccagggctct ccactatctt gaggactgcg gaacaccgta tcacgcctct
     7441 ccgggcactc ccatcgaaat attcaagata ccctttctgt acaggaagca gttcaagaaa
     7501 atcggctttt atcacaaatt tcacgacaga tacctgggct accgattgtg gagaggttac
     7561 gaaccttttt tggaggagat agaggggact gaggccggcg tcttcgatag gctcgaaaaa
     7621 atggcgaaga caattagaag gagagccttc gatcttctgc ccgaggtaga actgtctgtg
     7681 aaaagactta tcggcggcaa ttcattcgag aatccatcac cagagttgtc aaaggagtat
     7741 tatggcaaac tcgtcagttc ccaggatacc cgtcgactgg atgaagtaag ctgcaagata
     7801 cttcgtaatc tctcttcggg agtgaagttt tacctgaatc acctggaaag aaggcttaac
     7861 tgaaagtcgc ttggaacttc cttatcactg gaaaagcggc ctggattact cggtgaattg
     7921 aaacccctct tcgaaaagtt caaggcaggc ctcgacgact ctgcgatcgt agaaccttcc
     7981 ggagttctgc cgtatttcat ccagtgccgc ctctattcca agagatgctc tataaggtct
     8041 gtgagaagac attgcctcaa ctacatcggc aacggcaata atacgggcct ctattctgat
     8101 ttcatcgccc gttaatcctc ttggatatcc ggatccatca agtctctcat ggtgttgagc
     8161 aacgatgtca gctatcggcc atgggaagta aacctctttc agtatgtcgt accccttctt
     8221 tgaatgctct ctgactaaat cgtattcgag attgttcagt cttaccggct tcgtcagtat
     8281 ttcggtgggc actgcactct tccctatgtc atgaagcaaa gcagctactc ttatcgattc
     8341 aacgatatct tcatgcatct catcttcaga aaacagcttt ctcgctattg ctgtcgaaag
     8401 ctcgcttaca cgtttctgat ggccggaagt gtagggatcc ctcgcttcga caagcgaaga
     8461 caaagtattt ataagtgact caagactctt tttcaccgat tcctcggcga tctttctcac
     8521 ggagatatcc aggacaatgg caacaaagac acgttcgtat tcattgtcgt gaagctgtag
     8581 tcttacttca acgggatatt tggagccatc ctttcttatg tgaacggttt cgaagacttc
     8641 gtaacttccc ggttctcttt tgagcctaaa gagaatatct tcgaaggccc ttttatccat
     8701 ctccaacttc agatccagtg gagtcatctt tcgcaactct tcaagagagt accctgaatt
     8761 ctctagagca cccttgttcg cttgaagaaa tctcagagtc tctgaatcga atatatatag
     8821 ctcattcact gaaagatcta tgactcttcc caatctggtg agggccatct ctgcgagctt
     8881 ccattcggta atatccatgg cgattgcacc gaccatcggt ttatctcctt ctgaaacaat
     8941 tggaaacttc tgaacataga ggatcctttc cttgccgtcg gtaccgagta ccctctcttc
     9001 cctcacgatg ttctccccgt tttgagtcct tctatcatct tgaagaatca gcttgtcgag
     9061 ttctccggga tagttggctt cgggattctt tcctttccag tcttctttga aaccaaagac
     9121 tctggctgcg aagtcattga agtatgaagt cttcaggtca ctgtcagaaa tgaacacggc
     9181 tccgggcagt ttatccatga aaagagaaaa cctcttctgg agaactctct cgttcttctc
     9241 aattcttatc ttatggatcg caaaagaaat atcatccacg atttcggaaa agagatctct
     9301 ttcatccttc gaaaaagaga agctctgttt cataactacg gccatagcac ccaggatatt
     9361 gccaccgatt ctgagccctt tcgcgaagac tgcaagatca tctgaatcgc tgattgttac
     9421 gcattgtttg ctggactttc ttccagttaa ggagatcact ccttcttctt ccattgtcct
     9481 tctcaaaaga ggaggaattt ctcccgactc gaaaatggag tcgacttcga gcagctgcgt
     9541 tctgctgaag ccagaagcgg cccagttctt cacatctcca ttctcaacga gaacaagaag
     9601 acatctttcg aaaccgcggg aacccgtcat aagctccgtc gcccttttga tcaactcttc
     9661 aacatcggtt gccgatacta tcagctggtt tactcttcta atagatgata gaacaccgct
     9721 ctgatgctct agctgagcct ctagtctcag tgacagtatt ccataggaga tatcttcgcc
     9781 gagctcttcg agaaggagaa cctcctcatt atcaaatgca tcttcctctg tggaataaat
     9841 aacaaagccg ccgatgatct cttttccaat aacaaggggc acggcaatcg atgacttcaa
     9901 cccgtacttc agaatcgttt tcttccaagg aagatagtct ggtgaactgt cgatatccct
     9961 tgaaatgaat gtcctgccgg tttttatggc ttttcctatt gcgcttctgc tcaattcgct
    10021 gttatcccag gcgactgcaa cttcgtaggg ataacccttt gccactccgc ttaccgcaac
    10081 aggtgtcagc atttttcccc cggacttctt gaaggctatc cagacaagag gaaaccctcc
    10141 gttttcgacc acgatgtcac aaagtccctt cagtaaagaa tgttcatcct ttgcatggac
    10201 gagaaattcg tttgctctga acattagggt tagcaatttg ttgagtttct gaagtttaat
    10261 ttcgtccatc ctgcgctggg taagatcggt gtacattccg accatcctca agggttttcc
    10321 ttcactgtct cttgagacaa ttcgtgctct atcacgaacc cagacaagag aaccgttctt
    10381 ccctattagt ctgtactcct tttcatagaa gggtatatcg cctttgagac atctattgag
    10441 aacagacttc acctcttcaa catcttcggg atgaattctc ttctcccatt cgctgaagtg
    10501 atcagagatc tcttcgtcag agaaaccaag tatctccttc catcgcttgg agaaaataac
    10561 cttgccggtc tcaatatccc agtcccatat gccttcgcca gaagcatcta ctgcgaaagt
    10621 tagtcttgcg agactttcct tcagttcccg ctgaatgatt actctctcaa gggtatttga
    10681 aagcgcaaca gctactcccg aaagaagttt cttttcttcc ttaagaaaag gacttccctt
    10741 tatgtcgagc tcttctgtta aggagacact tattgttcct attttctttc cgttcacaac
    10801 gatttccgct gaaatcattc tttcatcaga ccattgatcg actgatctat gctcatttcc
    10861 atcgatctct attcttatcg ccgtcagttc gggaaatcta aatccccgag gaagctgttc
    10921 caatgcagat acaactgctt catcggcagt cgaagactta tgaagctcat tgaatattct
    10981 gtaaacgcaa tccaattcct tcatccgctc ctgaagagcg aaaactgatc ttgccaggtt
    11041 gtcgctgcct tcttcagtcg acactataat acctctctct actttagtaa tagagtgatt
    11101 ctagcattaa aaaccttggt agcaatctaa tattcaaaca atggaaagcc gtcccagatt
    11161 aaatgggata actcttcagt ctgccctttt caaggcgagc tactgatcat ttcaatacgg
    11221 aatactttat cgaaattttt gggcagctct ctttgcattt aagacataca agacattccg
    11281 aagtgtcaat cttcattcca tcttcttcgg tttcgattgc acttacaggg caaacattct
    11341 gacagatccc gcaggaaaca cacggtggtt tttccacctt cattccaaat aacggttttc
    11401 ttgaaaagag agacagcaag gttccaaagg gacacatata cctgtgccaa aactcttctt
    11461 cgaataccag agttatcaga acagagaagg cagttatgta tagaagaagg ttgaccttga
    11521 tcttaaagat tcttaccgct accataagca aaaggaagag aacaagtata gaccatctga
    11581 atacattgtt cttgaagatt gctggtgtct ttaacctctt cactctaagc tttgaataga
    11641 gccaatttat gggtctgaaa agtgtttcca ttgggcagat ccacccgcag taaaaccttc
    11701 ccagaaatag agctccgacc agaccgatcc cgaaaattaa gaaccacttc tggagatccc
    11761 tgctcctcag aagaaaaaag aataggaaca ggaagccaac ctctacaatt actctcagag
    11821 ccgcggcctt tctatttgtc atataaatct ctcctttcaa tgaaagtcta tagcaaaagt
    11881 aaggtcaagt acgtgatata agtcacgaac gtattagagg aggcgatttg tgaataggtt
    11941 cttgatggta tagtctttac agtccaaact aatggcggtg atccgatgag aaaaatcgac
    12001 ggcagtgagt ggaggaatct tcttcccttt ctttacaaaa acagggagtt cagcatgttc
    12061 attatcggcg atatagagaa tacaagtcct gattcggaca acatggagat ttacctagat
    12121 ggagactttg acagtcctag aggcattctg cttcgatact acaaatttct cattctttcc
    12181 gacggggcgg ggatgaattt cagagaagcg gcaggcgttg tgaggaggta tgaacaggct
    12241 atgatgctta gcggaaccat ctcgggtata gataggatat ctccgtattt gcaggagata
    12301 cgtgaagagg aagaaactct ccgcttcgca gttttgaggg aacccaatct taatgcggcc
    12361 gggtttgagg taagaagggc tactactgag gatgcagaga agcttctggc ctttcttttc
    12421 tcaatagaag agttccacgc aacggatgaa gaatccttca tggccacact gaaagacggg
    12481 agcacaagaa gatacataat cgaagatcag ggcgaaatac tggccacggc ggccagtacc
    12541 tccgagagtt ccgatatggc aatgattatt ggagtcgcaa ccggcaagga tttcagaggg
    12601 cgaggtcttg cttcggcagt tgtatccaag ctgtgtgaag atctactcgc cgagggaagg
    12661 acaccatgct tgctatacga caatccagag gcgggaagaa tctataatcg gctgggtttt
    12721 agagagatcg gtaagctgaa aacactcagg ttcaaaaaac gatgaccttc agaaatcaag
    12781 ggctacgccg atctgaccca ttggaacgaa gctcgtttcc ggtttcgtaa ttcgctgata
    12841 cagtccaaga ctcatccaca gctgaaatcc tgctttgaag ctggtattta aactccattt
    12901 gatctcaaga agcattcccg ctatggcagc caaatcgaaa gcgtcggaaa atatcgctcc
    12961 ggcacaacct cttccaacag ctgatattat catcttctct ccgaataagc tcaaattacg
    13021 atctgtaact tcctgtaagc tagaaagacc agggaccgcc tgaaccaaat atcgtaattg
    13081 taggtatcgc tcccactccg cagtaaatat tggagtccag ttccagagag aaagcgatta
    13141 tcgctgggct tcttttccag ctctcagtgt tagcccgggg actgccacaa agaaaacgag
    13201 aaagaaaaag atcataacaa tcgttttcat taagaaaacc tcccatctca gcgagattac
    13261 attccagttt gaacgactaa cgatacgatt ttgtacacat ttgacggggc agttcaatga
    13321 tagaatctta atagtgactg agagtgaggt gttattcagt ggagccaagc gagatgtcgg
    13381 ccagagaggt tctaaaggcc tttttcgaga gctacaggaa tcaggatttt gaatcgttga
    13441 agtcactttg caccgataat attacctaca tcaatcccga gggactatcc aataatggca
    13501 tagatgagtt tctccagctt ctgaaaagag agttcgaatc attcggggtt cttttcgaac
    13561 cgatatcctg ggaatcctct gttgaaagac cttcattctg ttatttttca tggaaaagag
    13621 gcgtcaagtt tgctagaaaa gccgccttaa ggagagttga gacttttggt tcggcctttc
    13681 tactaaaggt tgagaaaaag tggcagcttg ttcacttcca gcaagcgttt tctggttctt
    13741 acgcgagcct attcaggaca agggaaaagt aatcaccggt ttttcatcta agaattggtt
    13801 gatcgtatcg cacagttttg gaatgatgtg gagttgttgt aaaatactac catagtacta
    13861 cgaaccgact gaacagatct gcccggcaat taacgtcatt ttcaggagga caaatctgtg
    13921 gataatacaa agaaggcctg gctcgtagtc ttcagtggtc ttggagtcaa ccttacgctc
    13981 ggcgtcctct attcatgggg aattatttct gcggccctga tagacgactt caaatggact
    14041 gcaactcaga ctcaaatccc ttatatgctg gcttctgcgg ttttcgctct cactatgatt
    14101 cccgggggaa agcttcagga caagtttggc ccgagacccg ttctctttgt ttcatcgatc
    14161 cttgccggcc tgggtttctt cttttccggc ctgaaccttt cggttgcagg tctgacgttt
    14221 ttcttcggtc ttgtcttcgg tcttgcaatg ggttttggtt attcatcgcc aacgccggcc
    14281 gcagtgaagt ggttccactc cgaccataga gggctaatat ctggaatcgt tgtcagtggc
    14341 ttcggtctgg caccggtgta tattgcaccg ttgacgtcag gcctcattgg tctgtttggt
    14401 ctgcccacgt cccttatgat actgggtctt ctattctttc taatagtctt cactctctcg
    14461 ttcttcataa gtaatcccca agagggagtc gaaaaggtaa gactgaaaaa gaagccgaga
    14521 aaggtccata gactgacctc aaaggattac acccttaagg agatggttag aacgccacag
    14581 ttctacatac tgtggaccat gttcttcttt ggaacatttg cgggattact cattattggc
    14641 cagatgtcta agatcggtct tgagcaggca tcgattagta acggctttct tctcgtggtt
    14701 gtatatgcaa tcttcaattt cattggccgg gttacctggg gaagcatctc cgactttatt
    14761 gggagaacgg ccacactttt cgctatgttt gccatacagg cacttgttta cttcctgttc
    14821 agctcgttaa caaatcctct tgctttatta ataggcaaga gcgttgttgg gttcacattt
    14881 ggtggaatgc ttgcaatctt tccggttgtg acggctgact tttacggtgt taagaatctt
    14941 ggagtgaatt acggtgtaat gataacggcg tggggtgttg gcggagttat cgggccattg
    15001 ctcggaggaa tcgcccgtga tatcaccggt gggtatgaga taagctacat agtctcggcc
    15061 gtgctaagcg tagcgggcgc tctgctgagc ctggtcatca ggcatcccga agaagaaaaa
    15121 cgagagaaaa atgggggaag agccgatgca aaagagaact gaaaagctta aaaagatcag
    15181 gaaaacgctc gccgtgctgt ccgacgatgt tcagtggaaa ctcgaagacg tagttcttac
    15241 gatcggagac ctaatagatg aggacaagaa agtaacgatc gatgtcctaa gagagatcgg
    15301 ttcgatggat tcgcgcagga agaagctctt catgctctat atgaaagcat ccgaagagag
    15361 agacgccctg atgaagcaaa gaatcgaaga tctctggagt gttgaagacg atgagctgga
    15421 agaggtgctt aaggaattat cgaagcattc aatagatgac ggtaaaactc caaaagagcc
    15481 tccttcagta gattagaggc tcttttcgat gtatttcata agcgtctcca gttcacctat
    15541 cttctcatcg acatttcctg aatcaatagc ttcccttatg catgtttcga cgtgtttgtt
    15601 gacaacggtt gtgtttgcct ttttgagaag agagataacg gcgaggagct gtgtcgaaat
    15661 atctatgcaa tatcgctctt cttctatcat ctttatgata ccgtctatct gaccccttgc
    15721 tgtcctgaga atcttcaacg cgccgctgtg tttgtgatga gaatgattat ccatcttatc
    15781 tttcgattcc ctttaacaaa tagccggcat cttctacggc ttctttcagc aacctgtctt
    15841 ctatttcctt tgaagtgcta atcgttgcca ctccggttcc aagatcgact tctgcgtcct
    15901 tgactccttc cagagccttc aatgattttt caaccctcat cttgcagtgg ttgcagctca
    15961 ttccttcaat tgtcagtctc attttcttcc tccctttcag atcttaattt tcttcaatct
    16021 taatgcgttg gttactacac ttacgctaga aaaagccatc gccgctccgg ctaccattgg
    16081 attaagcctt agtccggcaa ctccgtagaa gagaccggct gccaaaggta tgccgattac
    16141 gttgtagaag aatgcccaga agagattctg ttttatgttc tttatagttt ccttcgataa
    16201 ctttatcgct ttgaagatgt tgcttaagtt gtcgctcata agtaccacat ctgccgtctc
    16261 aatagcgatg tccgttccag aaccaacggc aattccaata tgggcctccg caagagccgg
    16321 agaatcattt attccatcgc ctaccatcat cactgtctta ccttccctct tgagatcgga
    16381 aacctttttg gctttgtctt ccggcatcac ttccgatatt acattctcga tcccaacttg
    16441 gttagcaatt gccattgcag tcctggagtt gtcaccggtc atcatgtaag tatctattcc
    16501 cattttcttc agagacttaa tcgcatccag agaatcctct ctcacaacat cagcgatacc
    16561 aagcactgct gccgctctac cttctatgga gacaacaacg ggtgtcttac cctcactgga
    16621 taactcatcg acaaacttca tttcttccgg cgtcagagaa ataaaacgat ggtttcccat
    16681 cttgacttcc tgcccagaga ctctcgccag aattcctttg ccagaataag cctcaaatga
    16741 agaaacctcc aggagaggac cactatatgc accggagata gccacatcga gcgggtggga
    16801 actctttgaa gcaatactgg ccgatatttg cataaccctt tctctttcgt gttttccaaa
    16861 tgtgtgcaca tctactaacc ttggccttcc ttccgttatc gtaccagtct tgtcaaaaac
    16921 tacagcacca attttgtgag tggtttctag ggcttcgccg ttccggaaaa gaattcccat
    16981 ttcggctccc ctaccggtac ccaccattat cgcggtaggc gtggcaagtc cgagtgcgca
    17041 gggacaggct attacaagca ccgctatcat catggacatt gaaaagacga gaccatatcc
    17101 aagcgtcatc cagacaagga aggtaactgc cgcaatcact agaacgaatg gcacgaaata
    17161 accgctgacg acatcggcca ttctggctat cggagccttt gacgactgtg cgtcctcgac
    17221 gagtttgatt attctcgaaa gtaatgtgtc actgccaacg acagttgccc tcatttcgaa
    17281 tgctccattt atattcgtag taccgccaat tactttcgaa ctctctgaga tatctacggg
    17341 aatcgattcc ccagtaatca ttgactggtc tactgagctt gcaccattca agactttccc
    17401 atccaccggg attctcatac caggtttgac aagaagaacg tctccaacct ctatctcttc
    17461 aatcgctact tcttcgaaga cgtcctgccg ttttacgaga gccgtttcgg gagagagatt
    17521 cataagtttc attatggcat cggaagttct tccccttgaa agactctcaa gatacttgcc
    17581 gagagatatt agagcgataa tcactccggc cgattcaaag tacaaatctc ctacgtagct
    17641 gtgtctaccg agggctattt gaaccgtcgc aaagattccg taagagaatg ccgctcccgt
    17701 ccctagacct actaaagtgt ccatatttgg gcttcctctg aagagatttg gtattccctt
    17761 ggaatagaaa tctctgcccg caactactat tggaatcgtt agaattagct gaactatggc
    17821 gaagttaagg ggattgagat ggggatcgat gattgccgga agcgaaaggc ccatcatatg
    17881 gcccatggca attatcagca gcggcagagc gaaggccgca gaaaatatga atttcctctt
    17941 gtaggatgct attgcagcct cttttttcag tcggctctct tcaaatgctt cagtgacgag
    18001 gttgcctggc ctatatccgg ctttttcaac tactttcttc acctctccaa ttttcaagat
    18061 gttgggatcg tacttaaaag aaacggtgtc cgaagataga ttgacactga cattctcaac
    18121 tccgtgcagc cttccgattg ctttttctac ggctgcactg caggcagcgc atgtcattcc
    18181 atcaatttcc attacagcct tcctaaaaac cggaatttct ttaagactgt atcctgcccg
    18241 ctctactgcc tcaaatactc tgcctttatc gacttcctgg gacacttcga atgtaagacg
    18301 ttccgtagcg agatttacta caggcctctc cactccttcg attttagaga cagctttttc
    18361 aacggctctt acacatgccg cgcaggtcat gccttctaca atatattctt cttttacatg
    18421 attattctca aaactcatct catttttcct cagacagcgc ctttttcagt tgctcgtact
    18481 gctcaattga gatctctcca ttagccagtc tttctctgag aactctcatt tctttgtcat
    18541 atgaggagga gatagtcatt cgactcttca ggatatcagg cttcagaaag aatgctaccg
    18601 caaagattac tattaggaat ccaaagaaca tcatcgtatc accccttcca ttggaccccc
    18661 ctggggggag ggttattcta ataatactat aaattctgtc ggatttcaat acattacttg
    18721 aagaatttca ctgagcagta ttagtgcaag gatcactagt agaaccaata atgaagagag
    18781 tgcttgcatt cggttctact ctgaggtact acaaaatcac aggtcttgta gccgaccctt
    18841 tctcatgttg tcattatttg actgagtcaa cggagtccat tttcgagagt ttcgaccagt
    18901 ttcctgcaga agtcagggat gtctttcacc actcttcccc agactagatt gccatcaact
    18961 aatgccggct cgtctaccca aaccgaacca gcatttatta ggtcatcttt tattccttca
    19021 cttcctactg ctttcgaacc cctcacaatt cctgccgata ttccaacgag accggcatgg
    19081 cagatcatcc cgataacctt tcctctttcg ttggcatctc taaccagact cttcacctct
    19141 tcgaatcgtc taagcctgtc gggagcccac ccgccgggaa tcacaatccc atccattttc
    19201 gagacgtcta tcgatttgaa gtctgaatcc gaaaacgctt caagcccgct cttaccaaga
    19261 aaaagaatgc ccttttccct gccagcaatg accacctctg ctccttcttc gcgaagccgc
    19321 atcaccggta cccagaattc gagatcttca aagccgtctt caacaagaac cgctatcttc
    19381 ttaccttcta atctcatatt tatccacccc tttctaaaga gttatcaaaa accactccag
    19441 agtagcatcg atgatctcca gcactttatc ctgtctttcg aaaaggtcga aaatgtgacc
    19501 ggagtcctgt tgaagcagtc tgtctacacc tttgtggtac attgtgaaga gatttgagac
    19561 ttccggttgg ggaaagacca gatcgtcctt cgatccagag acaaccagaa gtggcccatc
    19621 gtaggaaacg atctcggcaa cgggatctat aagaaacagc tcatcaaaga attcctttct
    19681 gagaacagtt gttccacccc aggaaatctc tgcggtgatt tcttcgagtg gatctgcttc
    19741 aatagctcta tcaacggaat ccttaccgag aagtgccgaa tacgtgtgaa cgggtattgc
    19801 gactgccgac cagagggcaa cactcttgac tctggagtcg caagctgcga gacaggctgc
    19861 gacgagacca ccctgactga gaccgactac gccgactctt cggctgtcta aatcttccgc
    19921 caacgaaaga aagtcaatcg aagatatcgc atcggatatc tgtttcgtga aagtagtgtc
    19981 cttccattcg ccatcacttg ttccggagcc cctgaagtca aacctcaacg atgccaggcc
    20041 cttttcggcg aaaagccttg cggtcatcct gtagagggat tcttcagaac cgtacacagt
    20101 gagatcatcc atatgtccca ggaatccatg aagtagcaca acaactggca cgggtccttc
    20161 tgaagattct ggacgtgtca gaataccgtt tattctctct ccctcgacaa agaagtggac
    20221 ttcgctttcc gtgacgatat gatttgagtc tatggaagta gcgaaacagc ttatcgacag
    20281 tgaaatcatc aacatgatta gcaacgcact tagtagcgac tgtttcttca catgttcacc
    20341 cccgtgttaa ggacaatgat tactctaata ttctaccgtt tcgattaggt caacgcttta
    20401 gaccagggct gaagaatgaa cttctcaact tcctcgactg gaaatctttg cgcatggctc
    20461 gctgtattca ccagttgctt catgctaaaa taaacaaata gaaggtgaga tagtggccag
    20521 aactgaaatc gatgcccaga ttctcctggg tgacctcgaa agcattcttc aatatcgcct
    20581 caagaggaga gacaaagagc ggatcttcga cgcctatgag ttcgcgcgct tccatcatca
    20641 ggaacagatg agagactctg gagagccctt cataagtcac cccattgaag tcgccaaaat
    20701 actggcccag ttttccgcgg acaacgacac aatcattgcc ggaattcttc atgacatagt
    20761 cgaagactgc aatgttccac tatctgagat cgcagaaaaa tatggagata cggtagccct
    20821 catagttgac ggtgtgacaa agataagcaa cttgaagcta aatgagaagc ttgagtccaa
    20881 gatagtcaag tcgagaatga aagtcgagac attacgaaag atgcttctgg cactttcaga
    20941 tgaccctagg ataatcatag tgaaactggc ggaccggctt cacaacatgc gaacgctgga
    21001 ctttctaaaa gatcctgaaa agaagcgtga caaggcaaga gagacacttc aaatctatgc
    21061 accgattgcc cacaggatag gtatgcacaa aatacaggcc gaactcgagg atctctcttt
    21121 caagtatgag tatcccgaag agttcaagga actaaaattc aaggtcaaca gaaagcttaa
    21181 ggaacgtcag gacataatgg acgaatacaa agaaattgtg cttcgagagt tgagaaagaa
    21241 caggatctcc gccttgatcg aaggaagagt aaagcatctg tacagcatat ggcagaagat
    21301 gattaagaag aataggtctt tcgatgaagt attcgaccta attgctctcc gtatcgtaac
    21361 cggagatgaa gtcaactgct acaagatact gggagcggtt cactctcttt ggccaccaat
    21421 gcctggtaga ttcaaagact acatagccgc tcccaaatcg aacggataca agtcactcca
    21481 cacaacttta atcacccaca gaggggagcc tttggagatc caaataagaa gcgagagaat
    21541 gcacaaggaa gctgaatacg gtgttgcctc tcactgggtt tataaagaag gcatagatgt
    21601 aaaggataga acatggttta cgcagctcgt tgactggcag aaagatttca tagaaagctt
    21661 caaagatatg gaatctattt ctcgcgagct agaggtcgaa gaggtttttg tcttcactcc
    21721 aaagggtgag gtggttcatc ttccgaaagg ggccacaccg attgacttcg cttatgccat
    21781 ccatactgag gttggccatc actacgccgg tgccaaggtg aacgatcgtc tcgttccggt
    21841 caactatgaa ctgcaacttg gtgatagggt tgaggtaatt gttaacaagg caagcgaagg
    21901 tccgagtctc gactggctga aatatgtgag agcaaactcg accaaggcta agataaagag
    21961 atttttcaag aatgaatact cggcgaaact cgtagagagg ggcaaagaga tattcagaaa
    22021 gataagcaaa cgtcttgccg tttcgatgga tgacctcatt gagggagaac aaataaaggc
    22081 cctgatgaac aggttgggtg cgcacaatga aaacgatctt ttcagcaagc ttggtgatgg
    22141 atcggtaact atgggagagg ttttaagcct tcttgcgccg aaggaagaag agatcgagac
    22201 tcttcccgaa gagacggaac tgataaaaca gaaacaggca aagggtaatg aggttgttgt
    22261 agggggtgaa acgggaattg ccgtttattt tgctaagtgc tgcactccgc ttcccgggga
    22321 cgatatcgtt gctgtaatga gcagcagggg gatctcgatt cacactcgca attgtagaaa
    22381 cctgaaaggc ataagcaaag acaagcttgt ggaggcacac tggaaccttg acactggcgg
    22441 caaattcagt tcctggatcg ttgtggagtt tgatagtgct gatagaacca ttgtaaacaa
    22501 gtctcttgag agacttgaaa acaagaacgc aaaagtaatg aagtactccg ttgaagcagg
    22561 caggtgggga tatgatacac tgattgcgaa tatccttgtc aaggatgtag cccatcttac
    22621 ttcagttatg gagaacctca ggggtatgaa gggcgttcaa aatgttaaga gatacggagg
    22681 agtttcgtga gagctgtagt tcaaagagta agtagtgcga gtgtagttgt ggacggaaag
    22741 gtcacgggca agatagataa ggggcttctt gttcttgttg gaattggcag ggaagatgat
    22801 gagagagatg tggagtggct ggtagacaag acggttaatc tccgtatctt tgaagatgaa
    22861 aatgacaaaa tgaatctttc ccttatagat gtttcgggat cgcttcttgc catatcgcag
    22921 ttcacagtaa tgggtgatgc gagaaagggc aggcgacctt cgtttaccga tgctgccgaa
    22981 cctgaagtgg ccagggggct gttcaacgat tttctccaga tggcgtctaa gactgtgaga
    23041 gtcgagacag gaatattcca ggctcacatg gacgtagaac ttgttaacag tgggcccgtt
    23101 acaattttat tggattcaaa gagagtattt taggaggtgt tcttgtgaaa agatccttga
    23161 gattcattct tattgtgata atggtatcgg ctgtcggagc gatagttgtt tctgaaggcg
    23221 gtgctggtcc atcggagcct gcaaagattg catttgccaa tatgcagaag gtgctagaag
    23281 cgacaaaaga ctgggtaact ttgaacatcg attatcagaa agacacgaag ttttatcagg
    23341 gccagttaga tactcttagc caagaatatc aagatctggc aaattctgga gcgagtcaag
    23401 acgcccttct acagaaacag caagagatcc ttacgaagaa gagccagtac gaacaaacac
    23461 ttgaaacgac ttacaatgca aagcttcagg taataatgga acaggtcaac aagagaataa
    23521 aggattatgc tacgtttgtc ggaatagata tggtgctctc cagtgaggtt gtcgtttatg
    23581 gttcttccgc gtacgatgtt accgacgcga tcattgaata tatgaaaggt tttcagaatt
    23641 gagtgatcct tcttatctga aatgcgaaca tcttgacaaa cgctacggaa aaagaagagt
    23701 attaaaggat gtctcgctgg aggcatcctc tcttgaggtt gtgggactcc ttggaccaaa
    23761 tggtgcagga aagaccaccg ccttcaagtc tatcctggga attgtgattc ctaattcggg
    23821 aagcattttc cttgatggtg agaacattac acaccttcct attcacgaaa gagccaagag
    23881 agggattgcc tatttgcctc aggaaacgtc gatcttcaga ggcctcaaag taatcgaaaa
    23941 ccttacaatg gtgatgaagc taacgggtca ggaagacaaa tgcctggaaa aggcaagtga
    24001 aattcttgac gaatttggca ttctttctct gaaagatcaa actgcctctc tcatatctgg
    24061 gggagaaaag agaaggcttg aatttgccag aactatgact ctttcaccct cgttcattct
    24121 tctggacgag ccctttgtcg gcatcgatcc aatgacagtg aaggatattc agaagatgat
    24181 cagaaagctc aagagcaggg ggattggtgt catcgtaacg gatcatgatg tttcatcaat
    24241 agctaaagtg gtcgacaggt tatacgtact gtacaaggga gaggtcattt cttcaggcga
    24301 tcctgcggcg gttcttggag atagtttggt ggtagaaaag tatcttggaa gtgatgaatg
    24361 atgcttcacg taacggtaat aggatactcc ggttcaataa gcgcgagccc cgtgaaagag
    24421 ctccacggaa tttgcgaaga agtagggagg actcttgcta ggcacggaca cgttgttatg
    24481 acgggtggaa gagacggggt aatggaactt gtttctagag ccgtttcgaa agaaggggga
    24541 cgtgttattg gtgtgctccc cgccggagat gagggcaaca cccacaatga aatcaggatt
    24601 cgcactggga tggattttgc tttgagatcg ttgatactta ctaaatctgc agatgtggtg
    24661 atttcaatcg gaggtcaagc tggaactctg cttgagataa tctcctcata ttcttatgga
    24721 caccccgtta ttctcatggg tggtaccggt ggctggacag ataggatagt gtcggtgctc
    24781 atagatggaa agtatttaga cgagaggaga acgtcagaag tcaagagagc aaatagtatc
    24841 gaagatcttg agaggttact tgaggaggca gaggatggta aggtgtagat ttgcgccaag
    24901 tcctaccggc aatcttcacg ttggcggtgt aaggacagcg ctattcaact ggctttttgc
    24961 gaaaaatcaa aatggaagtt tcgtgctgag aatagaagat acagataccg agcgttccga
    25021 gaagaggttc gaggaccaga ttctctcttc catgaaatgg tgcggtctta attggtcgga
    25081 aggtcccgac atcggcgggg agtacggtcc ttatagacag agcgagcgag tagctctcgg
    25141 gttttatgat aagtatgctc aagagctcgt cgagagagga cacgcatatt atgcggtcta
    25201 caaaaagtcg aatcaggaag acgtgctgag aatctcaatg gagacgcctg atatatcggg
    25261 tgacgaagtc tacacaatta agttcaggat tccagagggc aaaacggaat tttctgatct
    25321 ctcaaaaggg aagatgagtt ttgagaacga aaacttctcg gatttcataa ttataaagtc
    25381 caatggcttc cccacctaca attttgctgt ggtagtcgat gatcatatga tggatattac
    25441 tcatgtcttc agaggcgagg atcaccttac gaacactcca aggcaaataa tgatctatag
    25501 agctctcgac tgggaaccgc ctacgttcat gcatatacca ctgattcttg ggagtgacag
    25561 ggctccgctt tcaaagaggc acggccacac aagcgtggac catttcagag aagaaggtta
    25621 tcttagcgtg gggcttatga attacctcgc gcttctcgga tggacggtcg atgaggaaat
    25681 tttcgattat cacgaaaagg tcaaggattt cattccttcc gatatctcta acaaaggcgt
    25741 tgtcttcgat tatgagaagc tcgaatggat aaacggaaag catttgagat tgctggatcc
    25801 cgaggagctc atgatccagt ttggattgtg gttgaagttc accggaagat ttgactatta
    25861 cgcgatcatt gagtccgacc aattctactc gagagaggta ctcacgatgt gcagggagaa
    25921 gataaacacg cttgaacaac tatttgattt ctctgctccc tttttcagtg atgagctcga
    25981 ttatcaggat gagtatgttt ccaagtatct tcagactaac tggagcaagg atttaatctc
    26041 ggcagcaata aggaaatttg aggattctct tgactggtct gttgaaggag tagaaaaaac
    26101 cgtaagagag cttgccgatg aaaagataac ttcgaagaag aatacttttc agaccttgag
    26161 agggggggta acgggaaggc ttgtgactcc gggacttttc gagacgatct ccgttctcgg
    26221 cagggatagg gtcctcgaga gactggaaaa ccttctcgag atgattgagt atgaggaagg
    26281 gaatctctct gattgaaatg gttgtgtcgc ttgcggtttc tgcgatactt ctgatatcgg
    26341 ttttcaaaat agtgaacatg tcgattgcga tgtcattcaa cattgtgaga gagacaaaga
    26401 gttacatgtc gctttccaga accttcgatg ttattgaaag agatctcaac cgatcaatcg
    26461 gaaacttttc gtggggaacc tccttcatta gcttcgacgc tattgttgac ggtcggcaca
    26521 agagaatcag atattacgtt tccggcgaca ggattattag aagatctgga aactcttaca
    26581 acacggtgac cgaatttgta aagtctgccg gtttttatgg agaaggagga agcgtaagat
    26641 tctttgttga tgaaggagaa atcctaattg gaattgaagg tggataaaat gaatccaaaa
    26701 gatatcagaa agatgcttca ggctattgag gaggagattt tctcaagagc cgaaatgaat
    26761 gtgaaggata tgctgaaatc tccatcggca tacgtaaggg ctagggcaat aaaatcagcg
    26821 gcggaacgcg atatgaaaat cgagaatatc gagtcttttc ttgatgatcc ttcgccggat
    26881 gtcagaaaaa acgcggtttc ttcgatggcg aaattcggtg aagatcgaga ttctatactg
    26941 aaggctcttg atgacccgtc agacctcgtt agaagcgcaa cggtgaagga actcgtcgag
    27001 ttcggttatg aggacgagga gttggcgagg aaaattgctg ccgatccttc aaaaaaggtt
    27061 aggaagacct ttgttatctc tcttgctgaa gccggagaga gcgaactctt acaggagttc
    27121 aacggagatc caagcaccga cgttcgaagg atattgtctg cctataatgg cgaactgcag
    27181 ctagatgaag aggtgctctc ttcgctttcg ggaaaattcc agaaacttgc tattcattca
    27241 aggctcggta aactggattc gacttcttca gaaaagcttt tgggtctcat aaaggatttt
    27301 cgcatttcaa agacaaagca gattctagtt gaatctttag aaccattccc ggaggaggtc
    27361 tcggttccgg cgctgagaaa gctagtcgag tccgaggatt caaacgtagc aataacagca
    27421 ctgaagactt acagaaaaat taagggatac gatatttcgt tgttgcaggc cgcagagagg
    27481 ttcatggatt cagatgatga agagatgaga ttcgtcgggg cgcagtacgt caaaggtcta
    27541 cttgaaccat ccgcggttga tattcttcga gaaggactta atgatccctc agacagggtt
    27601 agggcgatat gtattgaagc tttggccaat ctaatggaca attctatctc cgatgtcatt
    27661 gatgaatcat tgagatcaac ttcgtcgaga ctgaagaagg cttcactcag agcagtaaag
    27721 aaactcaaaa cattggatgt gggggatcac gtttcaagaa ttctttccaa caggaaggaa
    27781 gagcttcaga cgaggattct tgcttcttcc gtgacgggtc ttctcaaact ggacggtttg
    27841 ctgccttatc tcgaagagat agtggtagat gcttccagtg agagccgcct aagactatcg
    27901 tctgccagag caatggcaag aataaacccc tcacgtttgg ccgatctctt tggctttaca
    27961 gtataacaac cgctgtaata gagaccgccg tcgaaatcaa gataatcgag tcattaatcg
    28021 accagctcat tttgtcgagg cttcccaatg tatggccggg ctcgtatccc attatttcca
    28081 gagcggaggc ggtgtcctgg gccttcttta gcgaggtcgt aatcacgggt atcaccactg
    28141 agagagagtt tcttactctc ttgaaaaaac ttccgccatt gaattccaca cctctagcct
    28201 tctgggccat aattattcga ttcgtctgaa gcgataaaag aggaatgaaa gtgagggtta
    28261 gtgaaagtgc cattccgagt tcagaaaccc tcttagtttt catcggtagc tttccgacga
    28321 ttgcttcgat ggaggaggag atctccatgg gagttgtcgt atatgataat gtaatgctga
    28381 agaagactgc aaaaaccatc ctaacagtca ataaagtggc gttcaaaagg ccctcttctg
    28441 ttatcctcag tatcctcata tccagcagga cttttccgtg tgtaaagaag agctggaaga
    28501 tgacaattaa gagtagaaga aacttgattg tccagagggt tttgatgtac tcccccagcg
    28561 gaacttttga aagcaagaga agcgaaatcc aaaaggaaaa aaggatcaga gaatcaacaa
    28621 tgccgctcaa gaacaaagac cccaccgcaa gtgccacaaa agaaatcgtc ttgacaacgg
    28681 gagacagttt atgaatgatt gagtcggcag ggaagaaccg acctaaggga atctctacca
    28741 accatctctc tccctgtaac gaacaaagct ggtgggtgtt tcccataagg ctacttcata
    28801 gagctcgaac gaattcccgc gaatcttgcc tgagagttta ttgaaaatcc actctgctat
    28861 gttctctgca gttggttgcg atagcacatc atttatataa gagtgatcca gcacactcag
    28921 gacttcgtct ctgacaattt ctttcatttt cagaaagtcg attaccatgt cttcctcttg
    28981 cttctccccg gcaacggtaa cttgaagttt ataagtgtga ccgtgcagct tctcgcactt
    29041 accgtgatac atggtgaggt tgtgtgctgc gtcaaaagtg aactcctttg ttatgaagaa
    29101 catttctatg ttcaccctcc ttcctcgcaa aacatctacc cttgagaacc aaatatcgtt
    29161 ttctgtgtga cgatacttcg aatttcctgt cgaaatcatt cagtgatcac aagtataact
    29221 catcccaaat gggtattcca gctactccag tccggcccgt cagatgctcc acgatatagt
    29281 atagtatgcc tgctttctta tcggatcctg aaagaggaaa cctgctcttc tgaccaggaa
    29341 aaaatgtgga gaccatctca gcctggaatt agagacaaaa cgcagaacaa gaataaagtt
    29401 agactgcaat agagttctag aactaggttt gacatatatc taaccatatg ttatgatctc
    29461 agtgtaagat acatatcgaa ccgacggagg aattgataat gtcaaattgc actacaaaat
    29521 cgatgagaga agttacagaa cagatagaaa aggcacgagt agaaatcttg aagtccagac
    29581 ccttccttac ttttcagtag gactagagaa accccaagaa aaagagagag cctgaaggtt
    29641 ctctcttttt gaatgaaaga aacaaaaaaa gggtctctac ctagagaccc gaagtcatct
    29701 ttctttccgt gggggaaaag gggggttgct tgtatgtcgc gattctactg gtttatttct
    29761 tcagagtgca aaaaagtttc cgtatcttat agaagccgat ctggctttat cgatttcctt
    29821 ctggacatct tttccgattt tcaaagatcc cttcttcata tcgactcctc cttttcgtct
    29881 gttcgattca tctcttacat tttcatcata tcactacgtt agatagttgt caaactatta
    29941 ttgttaacgc acaataaact tctaacaata tatcgattaa gcagatttac gatcttgcac
    30001 tttcaaatgg atttgattcg acactgaaga atacttgtac atgttccgca tttttagtac
    30061 gttatagagt tgcgttgttg atgaattttc ctggttcaag gtttctcgaa ccttatccat
    30121 gctataattc tatacaagtc gaacttgaga ggtgtattcc ttgaatataa tcccgggata
    30181 cgttgaactc gtagagattt tgatggcatg tggtcttgct gacaggaaga gttatttcga
    30241 cgcagtttca catgatcttg gattcgattt cgaaccgggt caagagctag gggcatttct
    30301 tcttaagatc agagagaatg agaactggtc gataaaagtt gtggctgacg tattttcgga
    30361 aataaaggac agcgtcatct tcttttgcga ctttcccgaa aggccggggg gtgagattct
    30421 cctcgaggac tcaatcgaaa gccttgcgaa taacttcgaa gagaaatcta aatgtgtagt
    30481 aaacagcatt ctttcgaaca atcttaattt ctctcaagag gaagtagata agatacttga
    30541 aggggatcta agcattgtgc ccgaagctgt aaacaaggcc gaagatctct ctgaaaacac
    30601 gacatggttc attactcagc tgataaactt tcccaggcag tcgaaagaga tcctgctctt
    30661 tgctcttaac gaaatgaaga agtactacga agaatccggt ctgagaaaaa agaatcgcga
    30721 tcagatagcc gaaaaactaa agctcttcga aaatgatcgg tataatcaaa ttgccaaggg
    30781 atttcttgat ttttatggca ttaaggcaag agaagacttg ccacttcatc tcttgtttca
    30841 gaatacactg aagtattcag ggcttaagtt tgcaagctcc cgcaatatcc agttgatagt
    30901 tttcagcgag cacttcaagc agattgaaga gattatgaat ccatcggtta cggacaacac
    30961 tctcagactg tttctcagaa atctagccga tcaaaccaag atgcagattt tgaagtcgat
    31021 atccgaggaa ccaaaatacg tggatgagct tgcaaagctc gtagggctat ccaaagcgac
    31081 agtatcctat cacctttcga ctctgtccga acttgccttg gttaagagcc gaaaagatca
    31141 cagaagggtg tacttctctt tgaataaaga gcgtctcgaa aaaattctga aaggacttga
    31201 gacgctatac gatgaaggtg agaactgatg gaaataatca ccggctattt cgaaatatac
    31261 gatctgacaa tggccattca gttttcgctg aacactggtc ctgttgagaa ggtactcaag
    31321 acgctcgggg ttgattatga accctcttct cagctcttgg aattcaggga aacacttctt
    31381 aaggatgaag agtggtcaac gaggatgtat ataaccttta tgaacgaact gggtgttatg
    31441 gctcccatcc tcttcccaat aaggcagaag cttcaagacg gtatgcaggt gacgatcgag
    31501 gaaagcctta atgtaacgga agagggaatc gagttgataa gagaccgttt catccaggta
    31561 tttgcagata gaaggctcaa gatttcccat gatgagctga atcgtatgat aagcgaggaa
    31621 ccgcagaagg tctcaaaggc catagaggca ataggcggtg taagcccgga tactgtctgg
    31681 tttgtgcacc agctactctt ctttccgaag acggcgatgg atttcctttc ctccaagacg
    31741 atgaaaatct taaactatta tgaaggaagc ggtttaagag acctcaacac tcggctcgta
    31801 aaaggataca tagagagtag ttcgtcacag aagatcaaag acgccttctc gaactatctc
    31861 gactattacg atatcgtact cgacgagacg aaacctgtat acataactct tcaaaactcg
    31921 gtacctcata caaactctgg cctaatgaca tatccgacct ttcatcttct gataatgggt
    31981 cttgaagagg ttaccgaaga tttctcgggc agaatcccgc aggaagtcag gctacggagt
    32041 cttcttaagg tgctttccga cgagaccaga ttcaagatcc tgaagagact cagcaaggaa
    32101 ccggctttac agaaggacct cgttgaattc acaggcctcg ccaagtctac catttcctat
    32161 catatgggac ttctgttcaa gtcatcgttg atcgacgttg atccattcag cagcataatc
    32221 tgtgtgagaa aagagactgt acggagggca gcagccgata tcagaaatct tttgaatttg
    32281 agggatatga aatgattaga gcagtaatat tcgatatgga tggcgtgata atcgattccg
    32341 aaaagctcta tagaagggcc tgtaccgaac tggtttcgga gcttggtgga aagataagtg
    32401 atgagctctt cgagaagcag atgggtctta aaatgtctca aactcagaga gtaattgttc
    32461 agactgccgg tctggatatg gagcctgagg aatttggcag aaggtacatg gaaaggtatc
    32521 tcgaacttgc cagagagacc gtggttccaa atataggatt gattgagctt cttgattttc
    32581 tttatggaaa ggtagaactg gcgattgcat cgtcgacgga gaagtccgct gttgaagagt
    32641 tgatgaagaa aataaacgtg ctcgaatact tctcggtaat cgtcggggga gatgaagtta
    32701 gggaatctaa gccatcgccg atgatctatc aaagagcttc gcggcttttg ggaattgccc
    32761 cggaggattg cattgttatt gaagactcgc caaacggcat tagaagcggt ttcatggccg
    32821 gaatggaggt tcttggggta aggcacgagg agaacataga ccttgatttg tcgctttcaa
    32881 aacatgtgtt cgataatctg aacggggtta gagagtattt ggaaaagagg ttcgatagtt
    32941 taatttaatc gaagagaatt ttctcgagtt gttttgagct ttttatttcc acggaatttc
    33001 tatctggcat gaagatgatt ccatccttcc gcagttcaga aaagtttcga gaaacggctt
    33061 ctcttgttga tccaaaaagg tttgcgagtg tcttcttggt atgaggaagt tcaaatgaat
    33121 tgcttccctt ctcaagagat agttccagaa ggtagcttgc cagtttcttc ttgacggttg
    33181 ataatgaaag caattcaata atatatgaga gattcagtgt cttttcggaa atgctcttta
    33241 ggtaagagaa cagaaaacgc ttgtttttga aagcatatag gagagctttc ttcggaatgc
    33301 ttagtacttc cgaatcctct tccgcgaaga catgtgaggc gcactttctt ccagcgaacg
    33361 caagcgcttc tccaaaaatc tggttctttt caatataatt gatagtctgt tcacgaccag
    33421 agggaaatac ctttgcaatt cttaacctgc ccttgagaac tatacaaagt gaatcgcaaa
    33481 gatcatctgg agtgtagatc acctgatcgg gctggaacct gacagttcgc gaagccgaaa
    33541 gaacttcgct tttttcatca ctgctcaaat cgttgaagag atctactgtg tctaacatct
    33601 attcgccaac cctgtaccag tactgatatt cttctcgaaa gccgatctta gaatagagac
    33661 ttatcgcgct ctcgttgggt ttgtcaacct gcagataggc tgtctctgct ccccgttgcc
    33721 taccaagctc caagagtttt tcggttatct ctctcccgta tccttttcgc cgttccgatt
    33781 ctcgcaccgc tattccgaag agtcccacgt gattgctttc tattactgca aacccgcaac
    33841 caactgcgat tcctcttttt ctaaggatga tgaaggggct gccaggaaga agcatagaaa
    33901 gtagttttct tgcccaggct tgattttctc ttgccctgct attcagagag aagaagagtt
    33961 cgagccattc ctcctcgggc cttgaagcga catcggcgtc tgaaagatcg tggggttcac
    34021 tgcctatcgc tctcgtcatt accagtgctc tatccctttc ttcaaacccc atctctttga
    34081 ggacactgtt cagtcctgca ggctttgagt ccgcggtgag cttgaacatc gtccttaacc
    34141 cctttgactt atagaacctg ctagcctctt cgatcttttg ctctagaggt tcgaacccat
    34201 cgtagatagg atagacggag tttgacctgt ttgtatatcc gcctgcgtat ctcaataccc
    34261 agccatcaag gaactttgtg taaccggctg gccaggagtt taaggagatc tcttcaagcc
    34321 tacgaattct ggacatatta cctccgatca taattcttac ccaccgattc cggggcagaa
    34381 agcaaactct cttgctgtac aaaaacgaac atcctgattt tagtggcgca tatgaaaaag
    34441 caaaatcctc ctacacagaa ccgtacgaaa gagcgagagc aaattcaatc ggggaagtgc
    34501 ttgagaggtg tgactctttc aaaatacttc tcattgcaag caacgcttcg atccagaatc
    34561 tgtcttaagc attcccgtga gtaaacttac tgatgctttc cagtatcggt tcaatagctt
    34621 tcctgcttgt gaaatctact gccgtactgt aagccgccag cattcctctt tcatcgtcgg
    34681 aaagctcttc tccgcgtctc ttctttcctt tgagtttact cttcaataga atcatcaaaa
    34741 tcttatctac gagtctcaat ctcttgaagt caaatccgcc cctcaaatgg aagaactcaa
    34801 tcttctcgac aatttcaggc gagaagttct ttgcctttat gtcttcgata acctctgatc
    34861 tcgctggaga agctcccacg gagaagataa ttaccttctt attctcgaaa gatttgaaat
    34921 tctcttttat tgtagacaat ccactgatgc cagtggcgta gagtgatccg ccgaatacga
    34981 tgcagtcgta atccttaagt ctttcaggat cgacatcttc aatatcaaag agatcgccgt
    35041 ggagctcttc tgcaatccat tctgcatatt tcttcgaact tccatatcta gttttgtaaa
    35101 ggactactgt tttcgacaag ctcttctttt ccttctcctg aactgaagac tccacagcta
    35161 ttttaacagt attttgggat tactgcgtaa agatcataaa gctcgttcga caggacaagg
    35221 agtcaatttc tctatttgga tttaaatgca tgaacttgtt atccagagta ttctaatgta
    35281 aaattgagac tcttacctaa ctatgcggga ggcttgctat gattcgaaaa ttcatggcct
    35341 attatcggcc acatctaggg ctatttttac tggatatggc atgtgctttc atgattgctg
    35401 gaatcgatct ggtttttcct agattcacta cattggccct ggatgattat atcccttcgg
    35461 gtaacatgag aggtattgtg attatcgccg caataatggc gtttctcttt gtcttgagag
    35521 ccgtcttcaa ttacgttgtc agttactggg gtcacgttgt cggggtaagg atggagtaca
    35581 acatgagaaa ggatattttc tcccacctgc agacgctatc ttttagttat ttcgacaagg
    35641 tgagaaccgg gaagatcatg tcgagaatag tgaacgatct tcgagaaatc acggagcttg
    35701 cacatcacgg ccccgaagat gtcttcattt ctactgtaac gttgatcgga gccttcataa
    35761 tcttaatgca gaacgactgg agactcactc tcgttgtctt tgcctatata ccttttatgg
    35821 tctggtatgg agtgagaaag agacagaaga tggcaaaggc atttagattg gtgagaaaga
    35881 agatagcgaa cgtcaatgca cagcttgaga acagtatttc tggtattaga gtcgctcagt
    35941 cattcaccaa tgaagaattc gaaaaaagta aattcgatct tggcaaccag gaattcaagg
    36001 aatccaggca gttcgccttc aagtcgatgg ccgagatgac gaccggtgtc gacctgatga
    36061 tgaacatgtt gaaagttacc gttctggctt tcgggggata tcttacttac tcaggcgaga
    36121 taaccgtcgg agcctttgta gcttactttc tttacgttga tctatttctc cagccgataa
    36181 gaaggcttat gcagtttgct cagcaatatg aagacggaat gtcaggtttt gaaaggttcg
    36241 ttgaaataat ggaaacgaag tcaagcatca cagattcgcc ggatgcagtt gaactaaatg
    36301 gtgtcagggg agatataaga gttgagaagg tctctttcgc ctacgacgga ggtgaaaacg
    36361 tactcaccga tataaatctc catattcctg ccggtaagat ggtggctcta gtcggcccct
    36421 ctggtggtgg aaaaacaact ctttgccatc ttattcctag attctatgac gttaaaagtg
    36481 gacggatcac cgttgacgga gtggatattc gtgacgttac cattcactcc ttaagaaccc
    36541 acatcggtat tgttcaacag gatgtctttc tctttgcagg aacaattagg gataatattg
    36601 cttacggtaa gggcgacgct acagacgaag agataattga ggctgcaaag agagcgaata
    36661 ttcatgattt catcatgagt ctcgagaacg gatacgattc ttatgttggt gaaagaggag
    36721 taatgctttc cggaggtcag aagcagagaa tatcaatcgc gagggtcttt ctaaagaatc
    36781 ctccccttct cattttagat gaagccactt ctgcgctgga taatgagact gagctgaaga
    36841 tccaggagtc tctagaagcc ctttcgaaag gacgcacaac acttgtaatt gcccacagac
    36901 tttccacaat taagaacgcc gatgagatag ttgtgataac gaacaatgga atcatagaaa
    36961 ggggctctca tgacgaactc ctcgagaagg gcggacatta cgcggggctt tatagggctc
    37021 agttcaaggg gtatataccg gatatgtgat ctctccagta gatactcccg taaatatcgg
    37081 gagggttgag aggggtcaaa aaaaaggcat gttcatcaat ggctttggaa tactctgaat
    37141 tagcagatgg aagactctct tgaaagtctc tcagttctat gtcagcctgc gccttccgag
    37201 cgcaaaattt caattcgtga agtgaattat gaaaattcat tctcccgttt gtttgccacc
    37261 acttgagatc aaaggtttcg cgatccggct ccaagaggtg tattccattt tcaaacacga
    37321 ataagtcttt agctgtaggg ttatccactt gaggattatt tgttatactt aggctggtct
    37381 tggatctgtg gagggacgca tatggacata gagaatgatt ttctattcag atggtacgag
    37441 cggcaattac ttgttgaggg aatgaccagg tcgttcctgc tgagattggc cgaggagaca
    37501 tttggtcatc tatggggcgc gactgaggaa cttaccgccg aactcctaat aagaagtggt
    37561 ctttccggaa tcactagaga ttttccaagg cacagaattc tcccctttta ttcgcctctg
    37621 atttacaggc agaaagagac catggaattc agagaaggag acgatttcaa aatcgaggaa
    37681 gattgtggaa agagaattgt aagctttcca cacactctgt cgattatgtt ctcacctttg
    37741 atagccggaa aggttttttc ctggctcatg aatggaagca acgagatcgg tcccatttgt
    37801 gatctcccgt tagatttgca gagggcgtcc agaaagggaa gagtgatggt tgttggagcc
    37861 ggcggattgg gctgcccatt gataaaaatc ttgctggata acggaattga tgacatatgt
    37921 atcatcgaac cgggtgaaat taaacttaac aatttacacc gacaaatatt gtacgattat
    37981 ggagatgttg gtctttcaaa agcgagtgta attgaaaaaa agattgcaca gcggtacagt
    38041 ggcgttcggg tcaagatccg taaagaaagt ttcgatccaa cccttatcaa gactgaaaaa
    38101 cccgacgtag ttgtcagctg cgttgacaat tatgaggcta ggtatctgat aaatgattcc
    38161 tgctaccgca actgtgttcc ctttgtcgat tcggccgtag agaatttcgg cggatatgcg
    38221 atgtttagag ataaaagagt gccttgctac aggtgtttca tgggcgacaa cagaaaggat
    38281 acgggggcac aaaaggggat actgactttt gtaagctatt ttggaggcat gttagaggcg
    38341 gccatggtgt tgacttttct gaataccgga atgtgtggag acgatgtttt ctggtttgac
    38401 ctgagaaagg gtaaattcga gagcatttcc tttgaacgac gtgaaggttg tcccgtctgt
    38461 tcttcagcca agaggtgacg aagcttggaa gtagtctatg gaaacaatcg atggagtttc
    38521 gacagagatc ttaccttcag tgaactgata gaggaaacac agcttgggtc tgtctctcct
    38581 cttgttctgg tagacggaag atttgtcggt gaagatgagc tgatctcggc aaagtcgagg
    38641 gttctcataa tcgttgcagc caatgcagac caaacgggcg catagtaacg catactcacg
    38701 tataagctca caaaatccct tcttttggtc ttcatcgttt cgtggcggaa ttaggttttt
    38761 ttgtagggat cctcttttaa atgtagaatt ttaaaggata gttggaatat atatggaggt
    38821 ggagaaattg gaaagaaaaa aggttttgat tctcggcgct gccggaagag actttcataa
    38881 cttcaacact tactacagag acaacgaaga gttcgaagta gtagctttca cggcgacgca
    38941 gatacctgga atcgatggaa aaaagtatcc tgcggaactc gcaggcagac tctatccaaa
    39001 tggcattccg atagttccgg aagaagaatt cggcaaattg atcgacgagc acaagatcga
    39061 tcaggtcgtt ctcgcataca gcgatcttcc acatcaatat gtcatggaga gggcggcaat
    39121 agcaaacgca cacggtgcgg atttcattct tcttgggccc agcaagacaa tggttaagtc
    39181 ctcgaaaccg gtaatctcta tctgcgcagt taggacgggc tgtggaaaga gccagacaac
    39241 cagaagagtg ctcgacatcc tgagagccaa ggggaagaag gttgtctcta tcagacatcc
    39301 gatgccttat ggagatctcg tgaagcagaa ggtccagaga ttcgccgatt attcggatct
    39361 agataaacac gaatgcacta ttgaagagag agaggaatac gaacctcata tagatcgaaa
    39421 tgccgttata tatgccgggg tcgactacga agcgattctc agagaagctg aaaaagagga
    39481 tgtagacgta ataatatggg acggcggaaa caatgatttc tcattttata agacagatct
    39541 gtatataact gtaaccgatc ctcatagagc cggtcacgag actacatact acccgggatt
    39601 cacgaatctt atgatggccg atgtagttgt tataaacaag gaggagacgg cttctccaga
    39661 agatatcgac atcgtccgaa ggaatattgc gaaattcaat cccgatgcca ttgtagttga
    39721 cggtgcttca ccgataacca ttgagggagg caagtcgtcc gagataaagg gcaagagagt
    39781 tctcgtcgta gaggatggcc cgaccctgac ccacggtgga atgaagtacg gtgcgggatg
    39841 ggtagcggct aagaagtttg gtgcatccga gatagttgat ccaaggcctt atgcagtagg
    39901 atcactcgtt tcaacatatg aaaagtacaa ccacctggat cagatccttc cagcgatggg
    39961 atacggtgag aaacagatga aagagctaga ggagaccatt aacaaggccg acgtagatct
    40021 tgttataatt ggaactccga ttgacctgag aagggtaatc gacataaaga aacctgccgt
    40081 aagaatcggt tatgagcttc aggagatagg gaagcccgat ctagaagnnn nnnnnnnnnn
    40141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40201 nnnnnnnnnn nnnnnnnnnn nnnnnnnaag cctgcgatac ttgccaggct tcacgtagta
    40261 tctgcttttg aatgctctgg taagtgattc ctgcgaatca tagcctaact gcaaggcgat
    40321 ctccaaaata cttatcgagg ttgtagcaag tttttcggca gctacgctca accttctacc
    40381 cttgcgatac tggcctatcg tcttgtccac agtgtgaaag aagatccgct gaagatgaac
    40441 tgatgagtag aagctttctt agcagagatc gtcgagagtt atcggggatg aaaggtgctc
    40501 ctcaatgtaa tctatgacgg agttcacgca atcgatgtaa tcctatttca aaggagtgtc
    40561 tctggacatc ttccacctcc atctccgcaa taatcgtctt aaagattata caagtatgga
    40621 agggcttgtt tttgatcgaa agaatcatga tggagtttct tctctaagct gagtcaatcc
    40681 aaaaacaatt cggctgaacg aaaccgttaa tcacgccaac atcagtaccc aataagcctt
    40741 tcaagcttct tagccgcttc tattgtgcta cttacttctt tcggcagagg aggtattccc
    40801 tcgaaacccg tttttctgac gaagtcgacc agcatcgccc aaagttcctc agggttccag
    40861 ttagaaagtt tgtccaaggt atctaatcct gcattgcagt atagcctcac cctaactcct
    40921 ttcattccac caagtcttga aagatccgag agtttaaggt actcaagtat tttctccatt
    40981 tcagtcccga tgcgtgcagc caggctttct cttagctcag gtgaaagggc acttcgatca
    41041 tttgataggt ggcaaatatg ctcgctttcg cgagtttttc cattactatc gtgtcgtacc
    41101 agggaatttg tgtattctca ttctttccct ctttgccgcg attcctttct cacgaagctc
    41161 tggggcgaat tttgcgagtg aaggttcaga aagaaaactg aaatactggc ctatcctacg
    41221 gagttttgct tcagacgggc cttgtcattg cggttcgatt ttgcgaaact ttcaatatcc
    41281 tctttttctg cctcttcaac tgatttctca ttcgaatcaa gaaggaattt ctcgagggat
    41341 gcaaccccag acaacgttgt gttgataaca ttctagctct tacctttact cttcaagaag
    41401 gctatgaaat cttcgttttt tatcatccat tcatctctct tgcgaagaga aaacaccgaa
    41461 tcgatctgga gaaaaccctc tttagctact gaatgtatat ggtcatacaa cgtctcgatt
    41521 acagcgcttc tacagttatc ttcgaagcaa gactcattac atcatcgaat ctacaaagct
    41581 ctgtgccggg tgtcatgacg gcagctgaac tggcggcacc agccattttc aaggcagttt
    41641 tcaccgtctc gccaagttct ctgtacatgc agtatgccgc caggaaagaa tcccctgcac
    41701 caaccccgct cttgacaggt actttaggta cccgaatacg atagaactcc ccactcactg
    41761 atgcaatcgc tccctcggct cccatagtca gtagaatttc ttcgattgaa tacttgttcg
    41821 acacttcgga aatggccttt ctaaaatcta tctctccgat tagctcgcga ttgagcagtc
    41881 tctgaagttc gtgaatattt ggctttattc ctcttggctt ggcctcgacc cccttggaga
    41941 aaggctcctt gtcggaatcg atataaacgt tgactccaat tttcctcatc cttgaacaaa
    42001 gctgagcgta gatatcgctg cttactcctt ccggtatgct tccgcacaaa agaaggtagt
    42061 cgtcctttct caaaagtacg tcgattgtct tgtacagttt atagacttcc ttgacggata
    42121 ttaccggacc gggaagactc attctgtatt gaccgcttcc agtctgtacg agaatgttgg
    42181 tccttgtttc ttcttcactt cttaccgttg cataaacgac tccctcatcg tcgagcatct
    42241 cttctattac ctttccggta tgacctccaa ggaaagctac agcgaccgaa tggcctccca
    42301 gttcatttat cacacgtgat acgtctacac cctttcctgc ggggtaatct ttgactctat
    42361 ctaccctcac cgtatcgtcc tcaattagag cgtctgtata taagaatcta tctagagctg
    42421 cgttcaaagt cagtgagaaa atcaccattt acacctccta catcctgccg agacgatagt
    42481 ttatgattcc gagtactaca atcacgcttg tctcgactct cagagtcctt cttcccaacg
    42541 aaacgggcgt acatcgggtc gagatgtctt ttatctcctc cggcgcgaag cccccttcgg
    42601 gtcccactat cagtcgcagt tttctttcac aagcgagttt ttcttccact tctaataaag
    42661 gtctgccgcc gaattccaga agcaggttgt atgatccttc tagaggaagt gtctgggata
    42721 cagtaatctc gggaatgtaa ggattaacac tctgcttggc cgcttccctt gcgaccgcat
    42781 tgaacttagt cagcttagtt tcatcaactc ttgaaaccga tctaaggcta ttgaacaact
    42841 cgattctgtc gactcccagt tcggttgctt tctctatcaa aatatgaagc cgtggccact
    42901 ttgtcgctgc cacggctaca gtaatactct tcccgtctct ctcaacgtac tctcgctcga
    42961 ctacagttcc cgaagcagat gatttcccga gttcttcgag aagaaatctg taaacggtac
    43021 ctctcccatc aatgcctgtc agttcatcac cgggttttgc tctcgtcact ctcagatgtt
    43081 tggtttcacc gccgtctaga atgactctgt tgtcttcagg caggacgaag aaggcattag
    43141 gcaattaacc aattacctcc tgcctcatgt atggtacgag agctttcggc acgtcgatct
    43201 ttccgtctct tctctggtag ttctcgacta ttgctaccag ggtccttcca acggccagtc
    43261 cagaaccatt taaacagtgt acaaacttca gtttgttgtc aacatctctg aaccttatgt
    43321 tggctcgtct tggctggaag tcctcaacgt ttgagcagga ggaaatctcc ctataagtgt
    43381 tgtaagaagg caaccaaact tcaagatcat aagtctttgc cgcggcaaac ccaatatccc
    43441 cggtacaaag agagactacc cggtaaggaa gctccaattt cctgagaaca tcttcagcat
    43501 cggccgtaag attttcaaga gcatcgtaag agtcatcggg atgggtgaac cagacaagct
    43561 caaccttgtc aaattggtga actcttatca tgccccgaac atcctttccg tacgatccgg
    43621 cctctctcct gaagcatgca ctgtacgccg tgtacttttt gggcaggtca ccttcgagga
    43681 tctcgtccat gtgctgcgaa acgagtggga cctctgcagt tggtatcatg aacatatcgt
    43741 ccgggtcgat tctgtaggcc tcctcctcaa atttaggtag ttggcccgtt gcctgcatag
    43801 tatctctctt aaccatgaaa ggcagagcta cttcctcgta tccctttgaa tggtggagat
    43861 ccagcatgaa gttagctatg gccctctcaa gtcttgccaa gtctcttcta agaattgtga
    43921 atcttgctcc agatattttt gcggctcttt caaagtctat aagtccggtt tccgggccgt
    43981 agtcccagtg aggtcggatt tcaaagtcga cctttctcgg ttctccccac atccttatga
    44041 cgacgttatc ttcctcactc ttgccaacag gtgtggtcga cgaaggaata ttgggtagat
    44101 agagaagctt tttcttcagt tcttcttcta tctgagaagt ttggagatca agttccttaa
    44161 cttcagatga gatttctttt gccctttcca taagggaaag cgaaaggctt tcgtctttgc
    44221 tagctttggc tcgggcaatc tccgaagata tactgtttct ctcggccttt ttctgttcaa
    44281 cgacctgcag cgcctctctc ctctttgatt ccagatagac tatctcatcc agcagctccg
    44341 aggtcatctg cctgtactca agggccctcc tcacaatttc ggggttttct cgaatcaacc
    44401 ttacgtcaat cattatttct tcctcctgct aaaaaacaag ctctatctct acgtttttcc
    44461 cttcaaatga atcatccgtc gtatcgtata tcaggtcaga ggcccagatt ttcctactat
    44521 tcttgaagtc atcgacaaag acgtttccct ttaggttcag aaggccggta tctccgaagt
    44581 attcaaagta aagagtgtca acattcaatt ctcccttgta atttatgaaa ataagctcgg
    44641 gcgttgcttc cacagtttct tcagcaaaac cttcatagta ttccgtcttt tgatcaaagt
    44701 taagaatctc tgtctcgtat atgaatatat cctttccgtc ctcttccttt gaataaatga
    44761 ctgcctccac atttccgaaa agagttcccg tggagttttc cagattgtag tcgagggata
    44821 gagcagttgc ttcgcctgtc tggaatctaa caaaggtctc accttcggtt acgaagctct
    44881 cccaatcagt agcgcccttt gtggccgttg caaggggagt ttcgacgtaa atatctccgt
    44941 ccttattgat aaaaacgttc ccgatgaagg taaaaacctc tttttgaagc gatccaagca
    45001 ctgtgtctcc cttgattcgc acggtacttg caaagaccgt tgcggtaagg aaaatgacaa
    45061 tcaatccgat aatcagggta gttcttctct tcataattac ctccagacga ttagtctttc
    45121 gttttcttgc tgtccattat ttcgagaagg ttcttaataa taacctcaag ttctttcacc
    45181 tgatcggact tgatctcagt cattacagat ttcaagtact ccacccttct gtcgatgacc
    45241 ttctcgatta gtgtctttcc ggcggcggag atgtcgataa tgtacgatct cctatcttta
    45301 tcggaacgtc ttcgctctac cagatcagca tcgatgagcc tcttaacaag acccgttgtc
    45361 gtgctcttgg ttattccaag ccagcgactg atgtcactca tccgtttctc tccgttaaag
    45421 aaaagcacct gaaggacatc gaattgggca ggagtgatcg ggaagtctct caaaaccttt
    45481 cttccttccc ttctgattct cgtggaaatc gttcttaggt tcttctcaag aattgcagcg
    45541 tcttcctttt tgacttcacc aacggacatc acagcacctc caaacccctt gtattagttt
    45601 actgaaaacg atactattca aaacattata tcatcatcca tgtaccggtt aagactgatt
    45661 ctgcacctaa agctgttcat ttaacggttt tgatgccttt atagacatgt gctgaagcct
    45721 cctgaatggt gatgtataat ttcagtatga aatcggccaa acgagatcta tccgaacttg
    45781 tgagattcga tgcttcatac agagctgatt acagagttat tgcgggggta gatgaagcag
    45841 gaagaggccc tctcgccggt cctgttgtgg ccgctgccgt tatagtcttg aatcccgtcg
    45901 atggcgtgta cgattcgaag gctctatgta gaaagacgag agagttgttg tttggacaaa
    45961 tacttgagaa ctctatcgta ggaatcggtc tttcctctcc tgaggaggtt gatattctca
    46021 atgtctttgc ggctaccagg ttggcaatga acagagcatt gaggattttg tcgaggaagc
    46081 cagactatgt aatagttgat ggaaaatcgc ttaaactcga tataaagggt gaatgcatag
    46141 ttggcggcga tcgaagaagt gcagcgattg cgagtgcctc gattgtggca aaggtattca
    46201 gagatcgaat aatggattca ctggacatct tgtatcccga gtacggttat agaaatcaca
    46261 aaggttactg caccgatatg catctaagag ctcttagaaa gcatggaccg accacctggc
    46321 acagactgac atttaggcct gtcagggaat tgatcacaaa ggaaagggca agccgctggt
    46381 cgaatgagag tgaagtgagc caaatcaggc ttttcagagc ggggctggcg actatggagg
    46441 ccgacaaatg aagaatgaat ttgacggaag tgaatggtta aggctaattg agacaatgaa
    46501 gatattgaga tcacctggag gatgtgaatg ggatatagag caaactcatc agtccctaaa
    46561 accttacttg atcgaagaag cttacgaggt actcgatgca atcgatggtg gagatgataa
    46621 ggagctcgcc gaagagcttg gcgatgtcct tctgcaggtt gtctttcatg cgcagattgc
    46681 aatggaaaga aaggccttca cgataacgga tgttctaaag gtcttgacgg acaagttaat
    46741 tagaaggcat ccccacgtct tttcagattc ggagggttac tcatatcgtc agtgggagga
    46801 aataaaggca aaggaaaaag ccaaagattc gggaaagagt tcttcgatag gtgagataaa
    46861 taaggctctt cccgcgctga gcttggcgcg aagagttcag gagaacgctt ccgtggtcgg
    46921 ttttgattgg ggagaaatcg aaggaccacg cgaaaaattg aatgaagaga tagaagagct
    46981 agacagcgcg atagtcgaaa aggacaaaaa aaagatcgaa gaggaaatag gagatgttct
    47041 tttcacggtc acaaatctat ctagattcct tgatgttgat cccgaagcgg ctttgagaaa
    47101 atcaacagag aaattcttga accgtttcca cgaaatggag agtcatattg agaagcatgg
    47161 ccttgatatc aacaatatga ccatagatga gttgaatcac ctgtgggaaa tagcgaagga
    47221 gggtacaaat tgaagaagtt tatcgctgcc ctgatattat tattgatcac tgcagtagcg
    47281 ctttcgcaga cagaaaccct ttctgaaaca ggtatagccg ccgaggttaa cggtgagatc
    47341 atcaccatgg atactttcct ttcaaaggtt ttgcctaact ataccgagat atcgaagaga
    47401 atagaagagg tagatcctct tttttcggaa atgctcctca atactgaagc gggtcagaaa
    47461 ctgctagagg aatacgaaag aaattctctc gaagctctta ttgaagagac tcttctaatt
    47521 cagtatgcaa aagaggttgg gatcgaagcc gatatcgagg ctttaagaga agtagtaagt
    47581 aagtcaatca tggacacact caccgaactt cagattgaga agtcggatgc ggaccttttc
    47641 tacattctca aaggttacat cgacggatta agctcctatg aggcgaaggt ggtcagagat
    47701 ctcgcttaca aagaggttct aagttccttg tatgaagccg ttacagtgaa tgcgacagtc
    47761 accgaagagg agatagagca gtactatctg gccaattctt cgagatatgc tgtagaagaa
    47821 gagagggccg acatgaaggt gcttctcttt gattccttct cggaagccta ttcggtttgg
    47881 aaagctgcat ccagggatgc taacccttct tcggttttgg atactttccc ggaagtgcag
    47941 tccgggagtt atacgagaga agaaatcgag gcgctgaatc ccgaacttgt gaaatcgctt
    48001 ttcaaaccga cagaaggtga gttgctttct tccgtcgtga ctcttgacaa taagtacgcg
    48061 gtaatctatc tcgaatcata ttctcctgca ggagcaaagg gcattgaaga tgttagggaa
    48121 gaaatacagg ggatattgat ttcggaaaaa aaggatcttt tctggagaat ctggcaggaa
    48181 caggagttca agcctttcaa ggaagaggct gtcataaaaa gatactacga aacgggtgga
    48241 ggagaataag ttgagtgtga caaaagaaga cgttatgaag gcccttggag aagtatacga
    48301 tctcgaaata gggttcgatg tagtctctct ggggttgata tatggagtgg agctagaaaa
    48361 tgagaacgat gtcaaggtca agatgacttt gacgacgcct atgtgtcctc ttgccggact
    48421 gatgcttgaa gatgcaaggc gaaaggtcag cgagattgag ggaatcggcg atgtgaagat
    48481 ggaactcact tttgatccac catggtcacc cgagatggct agcgacgatg taagaaagat
    48541 cttgggaatg tgaatctaca agagctaatc catatatcga aaagaaagct ttcggaggcg
    48601 ggaataaaga attcccgctt cgttatgata tccctgttta ggggagttct ttccttgaat
    48661 gaagccgatc tgatcctcaa tggagagaag gaaatcgaat cgaagttaag cgcaaaatat
    48721 atgaaggccg tggaaagagt tgttgaggga gagccgatcg actacgtatt aggattcaga
    48781 gaattctttg gaatcaggct tggggttaca aggagtgttt tgataccccg aaatgaaacg
    48841 gaagagctag ttgagatagt catcgacgaa gagaagaatt cgaaagtgtt cgccgatatc
    48901 ggaactggaa gcggggcgat agcctgtgct ttagcccgca atattccttc ttcactggtc
    48961 tttgcaacag acatctctag agatgctctc atgcttgccg aaaagaatgc cagaacgaac
    49021 ggaatattca atattagatt cattgaaggt gacaacatat caggactgaa tgactttcta
    49081 gattctgtcg aggtcatcgt ttccaaccca ccgtatgtaa ggacggggga tctagattct
    49141 ctggacacca acgtgaggaa gtacgaacct atagttgctc tggatggagg aaaagacggt
    49201 cttgacttct acagggagtt tttaaaatat ctaccaacgg ggaaaaaagt ttatctggaa
    49261 atatctcagt atgagaccga gggtttaaga gagttagttt ctgaccttaa tggatattcc
    49321 tgtgaattca aaaaggattt gtctggaaat taccgtttca tgattcttcg tccagaagtc
    49381 tgagaacttc ctctgaaaag ctttcaagtt caactgagaa tatgtagtcg gcaaggtaat
    49441 cgaggccagt ttcatctctg ttgacaatcg cgactctggc acctgaggat tttgcaatta
    49501 ctggaaactg agcggcgggg taaaccatca aggaggatcc catagcaata aagagatcgg
    49561 acttcaatgc agcattctcg gctcttctta ttgcatcctg gggtagcatt tctccgaaga
    49621 aaacgatgtt tggcttgatc agtccaccgc aatcacatct aggaacatcg gatctttcaa
    49681 gcagtaactc gacttctttc cttgtaaacc gtttcttaca ctgcatgcat tcgaatttgg
    49741 aaacggtccc gtgtagctcc acaacttctt ctgcccccga cttctgttga agtccatcga
    49801 tattttgcgt gattatcgtc tcaattagac ccagggtttg tagtctggtg aggagaatgt
    49861 gagtagcgtt tgggcttacg tccgacatag tgtgtattct ctcctttgct actctatagt
    49921 accttgccgg atcttcaatg aaagagctta gctcaaagat gtccggagaa atcttgctat
    49981 atagacctcc cggacttctg aagtcgggaa tgccgctagc gacagatata cctgcccccg
    50041 ttagtacaga agtgcaggtc gattctctta tcagtgaaat gaagttcctt gcttcttgac
    50101 ttaccctact cacctctcga gaattgaatc ttcgggaaga tagtcttgcc cgtattggtc
    50161 catatgaatt catttccatc tgcggctacg gttggagctg gatctagatt aattaccttt
    50221 gcgtcggccg gtattgaaat tgttaaggaa taggcatcac ctgtaaggtc gaactccatg
    50281 tctcccatgt cggtattaac aacaccattt tcaaccgctg caaagcctgc gataacggca
    50341 tgttcgacta ctttaactct gttagaactc agaacagttg ccgttgactg gaagctctct
    50401 actaacatca gtctgtcgaa gctttccgaa aactgagtca tcgattcctg aaagtcggcc
    50461 agttgttctt cctgaggctt atcatattgc tcaaggaact gagtcatctg gtctggttta
    50521 ttgaagatta tctctgtctc gatgtcaagt gtcgcccgtc cctcgtagtc gtatgaatag
    50581 ttgctggttg atgacagggt gtctatgtcc agcctgttgt tgaatgccat aaacatattt
    50641 attgcggtca ttccaaccat gacaacaaca agtatgataa gaagggtatt gaatttcatt
    50701 taaaacctcc tgacaatttt agattgattc tgagaatatg ataacatcat tctatgctaa
    50761 aatacattgt acaaagcttt tcatttgcct ttgacgatcg gtaaaggttt ttatttcgag
    50821 tttccggttt ggagggattt cagtgcgaat tcttagcggt atgcggtcta ctggcagact
    50881 tcacctcggt cattttatca ctctggagaa gtggaaagaa ctgcaagacc aagggaacga
    50941 atgcttttac tttgtggcta actggcacgg acttacttcc catatagacg aaacctcttc
    51001 tttttacgat tggagtcttg acatagtgag aacatacgtt tcggcaggtc tcgacccaga
    51061 gaaatctgtt ctcttcattc agtctgcaat aaaggagcat gcagagctct ttctttattt
    51121 ctcgatgctc gtatcggttt caaggcttga acgagtaccc acttacaagg atcaaaagga
    51181 acagataaca gacagagatc tttcaagcgg cggattctta ctgtaccctg ttcttcaggc
    51241 cgcagatatt ctaatgtatc ttgccacagg agttccagtc ggtgaggatc agatatatca
    51301 cgtggagttg acgagggaga tcgccagaaa attcaacaat cagtttgccg aagttttccc
    51361 tgagccggaa ccgattctcg caaaggttcc aaagcttccc ggaacggatg gcaggaagat
    51421 gtctaagagc tacggaaact atatcctgat agacacagat gagaaagagc tatggaaaat
    51481 gatagctcca atgatgaccg atccggcgag aatgagaagg actgatccgg gagaccctga
    51541 aaaatgcccg gtctgggatt accacaaagc attcactacc tctgatgaag aaaagagatg
    51601 ggttgttgag ggttgcaaga cggcgggaat cggttgtatc gagtgcaaga ggcttcttca
    51661 caagaatcta gttcaaaggc tttctcccgt ctgggaaaaa ctctctcatc taaaagagaa
    51721 ttcagatgaa cttttcgaaa taattgaaga tggcaatagg aaagctagag tggttgcaaa
    51781 agagacaatg aagagggtta ttgaagcaac gaagttgggg tggtgaagat ggaacaactc
    51841 gaactggtct tcagtttccc ggaatttgaa ggaccgcttg atctactggt ttttcttgtg
    51901 agaaaacatc gggtggatat acgttcaatt ccaatcactg tcatagcaga tgagttcata
    51961 actcacgtca acaagatgaa atctcttgac atggtcgtca catctgattt cctcgtcatg
    52021 gcttcaaccc ttatggaaat gaagtctaaa gcgcttctcc caagaatgag tggcggcgaa
    52081 gccgaaacat ttgacaatca gagaaaggaa ctttacagga gagtagaaga gtataaagct
    52141 ctcaaggacc tttccaaaga atttcgggtc aagctctacg atgcttacag ctacgaaaga
    52201 gctacggcga aaccgacggt cgatcagaaa aggaaggaag atctccccga ggaactgacg
    52261 gaggccttca aggctatcct caaagagacg gttttgagag atagagttta tacgattgtc
    52321 tctgaaagta tcaacgtcga agatcgaatg cttcagatag agcagctata cgagacgata
    52381 gagctataca aatttctaat gagtctcgaa agcaggtcgg aaatcatcgt ctcctttctt
    52441 gcagtacttg agctgttgaa attgaacaga tactatcttg acacggtaga acctctaact
    52501 ctcaggagga aagcttcgtg agcgaggaac tgacaaaaaa ggcgataatt gaagcgctga
    52561 tattctctgc aagaagtgga atcgaaccat cgagaatcgc ttcgatcacg ggcatgaagc
    52621 tgaaccaggt aatgcttctc ctggaggagc tggaaagaga atacggaggt catgaacacg
    52681 gagttatgat caaatccgtc aacggcaaac tcaggttcta tactaaagcc tcaattcagt
    52741 catttgtaag tcagatatct gtaagacctt tagtaagcat cacagatacc cagatggagt
    52801 cgcttgcgat agtcgcagtt aagggcccgc ttacaagaaa cgatgtcgaa cttataagag
    52861 gtcgttcctc tcagggacag ctcctggagt tatcgaaaat ggggctcata agcaagagaa
    52921 aatctaagct acctggaagg ccttatctct acaaagttac ctcccgtttc tacgagttgt
    52981 tccaggtcga tgatcttaag gagatagtaa acggtttggc cttcagcaag gaggaagatg
    53041 atcttgaaac ttcacgagat aatctctcag ataacgaaca tgtcaaggag aaagtcgact
    53101 caagcgattc tggacgggag agtgaaagtg aacggccgga tcgagactta ccctaggctt
    53161 gacgtttttc ccgagagttc ggagattctt cttgacggaa gtagactgat tttctctaat
    53221 gaagagaaag tggtttatct cgtcaataag ccagtaggat acctgagtgc catgagtgat
    53281 gatcgcggaa gaaagacgct ctccgatcta gtctcagaac gaataaaaga aagggtattt
    53341 catgtcggaa gactcgatca ggacagctgc ggtctgatac tcatgacgaa tgacggtgat
    53401 ctttccaatc ttgtttccca tccggcatca gagatcgaga aaacctatgt agtaggaata
    53461 aagggaaaat tgaccgatcg cgatcttgaa gctgtgaagg gtgggttgac gctcggggat
    53521 ggattcatga cgtcgcctgc aaagataagg cttgtgagaa gcgacggcga cttttcaaaa
    53581 tatgcgatta ccattcatga gggtcataag agagagattc gggagatctt tagggtatta
    53641 aacaagtctg tatcgagcct tgtcagagta tccattggtc ctttgagcat atctctcgtt
    53701 cccaatcccg gtgatatcaa acgtttgagc cgaaaagaga tcgcatcact ggaaaacggg
    53761 gcccatcgca aggccccgaa gaaagctcag agaaagaata attttgggag ttagagttaa
    53821 agactagtcg gttagatcga cgacgaaagc ccctatatat gtatcggccg cgccgtagta
    53881 tagtatgagt ctatcttctt taatcacagc cccttctatg aatatcacgt ttggaacctg
    53941 cccttgtttc tcccatgaaa gttccggttc tgcaagaggt ttgtcggttc tcttgataat
    54001 ctttctggga ttctcgatat caaacaacgc agctccaggt ctataaatcg tggagaaatc
    54061 agcactgtta tatatcaaca gaatcccttc gtcagtaatg attggcgttg gccccggttc
    54121 aatcaacctg ctatcgaaac tcccctgtct tggttccatg acgggtctgg ggaaaattgt
    54181 ccagttcttc aggtctgtgg agtaagcgag ttttattgag gaatcaccaa aatacatgta
    54241 gtacttgttg tcgatcttcg caggtactat agcaccggac tttgaccaag tgtcatcttt
    54301 gaataccggt cccagctttt cccattctgc aaagtccttt gaagttgcga tgcaaagcct
    54361 agcctttcct ccatcgtatc ccgtgtaagt aaggatgtac aggtcgtcaa tcttaacgat
    54421 acggggatct tcgcatccac ccgggatttc atatggttct gttgggtata taagggggga
    54481 aggctctctt gtaaattcaa acccatcggt ggacttcgca aggccaattc tggatgtgcc
    54541 gttccatcta cccgtgccac tccagtcttc cgatctgtag agaaggtaaa ttgtagagtc
    54601 tatcattatg gcggccggat taaaaaccgc tttgctttcc cattcgatcc cctgtggcat
    54661 aagaagcgga aaatcagaag ctctctccat ggtgtataga gattcgaaat ctatcgaaac
    54721 ggcaacgcca attacacatg agagaagcag cacaattacc aaagatcttt tcatcaaatc
    54781 aactccttat ccatttcacc acggtctctt tgtcgattgt ggcaagagcg atatggttat
    54841 cggctgcgcc atagtagaca aagtactttt cttcatgttc taccatcgca tcggagaata
    54901 caacgttggg gacacctccg aaaatctccc actcttcttc cggttcaagg atcggttcat
    54961 cactcctctt tacaactttc tcgggatttt ccaggtcaag gagcataaat ccaagtctat
    55021 aagttggcct tggagtattc tctactccgt gataaaggac tagccaacca tattcagtct
    55081 ctattggcgg ggcccccacg ccgattttaa ggttgtccca cttaccagat cttggaccgg
    55141 ctatcggggt gaattccgtc caggtcctca gatcttccga ataagccaga tacatgtcgg
    55201 gttcgatcct atggaacatg agatatcgac cttttacttt cgccgggaat agcgcagcat
    55261 ctttgttatc gacatcaggg attatcactc cgtgacgttc ccacctgaga aagtcggtag
    55321 tcgaagccat agatattcgt acgccttttg gagagtaggc cgtgtactgc atgtagactt
    55381 ttccttcaat ttctgtaagc cttggatctt ccaccccgta aagctccagc tggctccctg
    55441 gagtgaagac cggtttttcg aagcggttga atctaacacc atctaatgaa acggcgtatc
    55501 ccattctcga aaccatatct tcgccctgag ccctataaat catgtgaaac aatccttggc
    55561 gttttataac ggcgcagttg aaaacatact tggattccca atgatgttgc gagatcggag
    55621 agaatagggg atttaacgga tgtctttcta gtttgatcac aactctcact cctcatttca
    55681 atgacataga gattccctga agaaagtact ttctgaacac cagaaatatt aagaccatcg
    55741 gcattgtttg caatacagaa ccggccagga ctggacccag ataactcccg tactgcatat
    55801 taaaacttgc aagtagtaca gagagaggca tcttcgaata gtcgcttacc acaatcagtg
    55861 gccacatgaa attatcccat attcctatga aagtgaagag acctacgatc gatacggttg
    55921 agcttgtcag tggaaccaat accttggtga ttatccatag atcattggct ccatccattc
    55981 tggccgcttc caggtattcg ttgggaactg ctctgaacga ctgtgtaaac atgaagactc
    56041 cccaagcaga gacgagtgaa ggtagaatca atgccgttct agtgtctata agacctagat
    56101 ttttaataag aacgtacagt ggaactatga atagaaatcc cggaaagatc atctgaaaga
    56161 ttatgaagtt gaatatggcg ttttttccct tgaagttaag ccttgaaagg gcgaagccaa
    56221 caagcgctga agataccaag acaccgaggg taactgtaac cgaaatgaag attgaattgc
    56281 caagagcctg aataaatggt ctctccatct tgtctgcggc ctcaagaatg aatctgtaat
    56341 tgtccagagt aaactgactt ggaagaaaac ctgaataaat ctcattggtt ggtttgaacg
    56401 atgagagaag catccataca taaggataaa tccaaagtat tgaggcggca aaaagaaata
    56461 cataaaggat tattgtcggg accttcttca tacaatctcc acctctctct cgaccagctt
    56521 ccttgtaagc aatacacagc catagctaag taatgcaaca actatagcca gtgttgaagc
    56581 gtaccccgga ttgatcttct ggaaggctgt cgtataaatc agcatttgaa atgtgtatgt
    56641 agttctcatt ggtccccctc cagtaatcaa gaatggttcc gtgaatattc cgaaagtcag
    56701 agtaagggag agaaccagta ccatgacaat cgacgggttt atcaaaggta aagttatcct
    56761 gaagaacttt gtcatctttc cggccccatc aagttctgcg gcctcgtaaa ggctctccgg
    56821 gatcgcctga agaccagaaa agagtattag accgtagtat cctatgaatt tccatgtgac
    56881 catgatcgct attgaaagag tggccagctg aggactgctg aaccatggaa tcgtaattcc
    56941 aaaaatcgct cttgttaatc cattcaaggg gccgtttatc gcgaagagct tttgaaaaat
    57001 tatggaataa gccactcccg atgagacatt tgctacgaga aatgcgagta tgaaaaacgt
    57061 tcggaatagc ttgactctct tcaatccaag ggcaaagagt aaggaaagac cgagaaccat
    57121 ggggataaag tacagcatga agttaaatgt attgaagaaa actctccaga acatttcgtc
    57181 ctgaaatagt tctatgaagt tgtcaaacca gatgaacttc cttggggtga agtaattcca
    57241 ttttgacaaa gcaagtataa caagccacac aaatgggtaa ccccagaata ttgccgtgta
    57301 taccagatat cccccagaca gagaccagcc ctttattgca tcgatggttt tcagtctctt
    57361 cctcactctc aatcacccct tctcctagaa gagttctctc cttactctcg tagcagagtc
    57421 tttaagtgca tctagcggag ttgattttcc atacattatt tgctctatca aaaatgttgt
    57481 gagaatttcc tgaacttcta cagtcttcgt agtcaaagcc ggtggaacgg cgtaggcaac
    57541 gtggctcgca taagcagcga acttcggatt gtcctccata tactcctgga agatgggatt
    57601 cgttaacagg tcttcccttg ccggaggaag attagtgaac tccatccatt ttctgtcttt
    57661 ttcaatatcc gaatagaccc acttaatgaa tttccatgca gcctccctat gctttgtggt
    57721 ggcaaacatt acaagcccct tggtatctgc gaaagtgtag atcggtttgt cctccggata
    57781 atcatctgga actggagggg gaacgattgt tatatgatcg aagacatcgg ggaagagatt
    57841 tctcgcctgg tttatctccc acggaccgaa gatccctcca aggacaacac cgaaatagaa
    57901 agggttgctt ccgagatcaa ctgccgtcca gttgtttctg aacatggtat ctataaatgt
    57961 aacgactttc tcgccggtct cattgttgaa aagtgctctg gctttttcaa catctagata
    58021 tggagctcct tcactcgcgg catagtagta ggttatgaag tcgtaccatc ggtcgtacca
    58081 gtttcttccc attaccacaa gtgctccata cttctcgtac ggcacagaat acttctcgct
    58141 tatctcgtag atctcagagt aggttctcgg cggtctgtca tacccaagtt ccttcaagat
    58201 gtctgatctc caccacatca ttatagggtt cgaatagatc gggagtacgt aatagttatc
    58261 tccgaacttc cagttttcaa cgatggaggt catctttctt tgctccacga gttcccagaa
    58321 atccgggaaa gagttgaggg gaataagtat atctgcttcg atgagttgtg cggcgaatcc
    58381 cgaaaatatg ttcgtacaga tatctggcat tcttccggaa gcgattgaag taaggatagc
    58441 ctcttctgag cttcccgcag cgggaatagt cgaccacttg atctggatat cagggttgtt
    58501 ttcattccac tcagccacta cttccttcca gaagctttct tggagaggat tgggcgctgt
    58561 ccagaagaca agttcaacgg cgaacaatcc cacaaagaga cacgaaaaca aagttaacag
    58621 aatcaatttt ttcatgtgat ttacctcctt ttttataaag tgctttctct ctgaacaaat
    58681 tcagtgaata gggagatctt tgttggatgc ggttcattac caattagcag actttgtagc
    58741 ctcctagatc caactactcc catctcatgc ttgaatatct tgaaagtagt caatgaggga
    58801 gtagtcgccg aggaattgac gatgtcatcg aaacccatga tcgacacctc ttccggtata
    58861 tttattcctc tggcctggag ctcttccatc gctcttatcg cggcggtgtc gttacatcca
    58921 aatacagcat cgggaagtcc atatctagaa agcagcagat cgattatcgc gctcatatta
    58981 tcattatctt catcgtattc ataagctttg ggaagatatc cggcgccttc cattgcagaa
    59041 atgtagccat caaagcgatc cttgaaccca aagtgcgaaa gtggtccgtg gatatttaca
    59101 atcttcttga gtccttttga aattagtttc ttcattccat atattgctcc atcatatcca
    59161 tcagatacaa cgcagtcgac ctttacgccg gcgagatact gatcgataag cacaaccggt
    59221 ttcttagtct cctttgctct tcttatatcc tcttcagtaa tctctccacc gatcagcagg
    59281 tatccatcgt agtcttccga aaaatcattt gaagggacaa gagttcccct catgttgttc
    59341 cccgcgaaaa actcttctat tcctttgagg acaattgtgt aaaactgatc ggattgatcc
    59401 gacaggacgc gaaataatct ctttcctgca acaattccaa tattgaacgc gagctttcga
    59461 cttgccagaa aactcgcgac ttttgaaggc ttgtattcaa gttcctcgac tgccagcata
    59521 accttttctc tagacttctt ggagacatac ccacttttat tgagcactcg ggagacggtt
    59581 gctgtagata cttcggccaa tttagcaaca tcagaaagct tagcggacaa aattatcacc
    59641 tcagtatatg taaacgctta catctctagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    59701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    59761 nnnnnnnnna acaatatgta agcgatttca tatcgacctt agggaggtgt taatatgaaa
    59821 aggttcttgg ttcttttctt catagtgttc tcagtttttt ctgttgctga aacagtgaga
    59881 gttccagtgt ctgtcagctc cttcactgga gaacccattg cagtaacaat atcgatgagc
    59941 gagatccttg atcttgttgg agttgacttt gacgctaact gggattcgct taaagtagtt
    60001 caggatggga aggagcttcc ttatcaaatt gacgataccg atcttaatgg caaactgtcg
    60061 tccggggatg ttatggcctt tctgattaca ggaccggcag aaatcacggt taccgacgat
    60121 ttcgatattc ttccaccgga gttcgtttca gtgggaaagg tcgtggagga agaaggacag
    60181 tggcttatcg agattggaga aataactgca gtagcgaata gcaagggtct agtaaaggtt
    60241 acaggctttg gagatgtaga gggcacgata gtagacgaac tgggaattgt gagaatgtcc
    60301 ggctacgttg gatctacata ctacgtagat ggtgaatacg ggcgacatga ggaaaagaca
    60361 agtggagact tcatagtaaa ggaagctaca gttcttccgg caggccccgt aggaataaca
    60421 gtagtcagca cgcttgaagc ccaaccgttc ctcggaataa cccaaaagat aatcacaaca
    60481 ttattcagca atggagatat catttctcat aacaccttcg aatttgcgac atacgccgaa
    60541 ctaatgaaac tgcaaataat ggctacaaga gtattgactg acgcagccga agacacggtc
    60601 catacactcc cggtattcag aagacttctt tgggccgacc agctgaatat tacgccgcta
    60661 gaatactggc ttgatagaaa tgcgatcatg ttttccggaa acaaacctta catagttttt
    60721 cctgctgtcg attcgatgag acctctctgg tggggtgcaa catacatttt tgcctcccag
    60781 gagagctgga gagccaacta ctcgcagtcg ttgaagactg gtgtagcaga gataaatcca
    60841 gaggttccag tcgtggtcgc cgactatgaa gagtggatgg gtggccttac ctgggtatat
    60901 gagagcagag aattcagaga cggatacttc gaatggatgc caggtgagat cgatatcttc
    60961 gagagtacga aagactacgt atcatcgaat tgggaagact atgtaaagca ctttgtggca
    61021 ggggatgttg tcagtttcaa gagggtttac tcaatataca gtgctgactc gatagaggac
    61081 tcaatagagt tcgttgaaat gaagaagtca gagattcaat cactcaagat cggagaataa
    61141 ctgatcctta atgaagggtg agaggcttcc tctcaccctt ttcttttgga ggtgcttatg
    61201 aagaaagcgt tgattattct agtacttctt atttcgttga tgcttttagg aattgaagtt
    61261 cagattaatc tggatcatct tgagtttctc agagatcatt ttgccgttga gggagaaagt
    61321 gaaatcggtt actggatcta tgcagacagg cttcctgatg gcaattacag gcatgccgac
    61381 gctccgggcg aaggagtgac atgcgtcgac gatgttgcaa gggctgcagt tctatatttg
    61441 aggctttttg aaatctctgg aatggaacaa cactacgatc gcgctaaaga agctctcgaa
    61501 tttgttcttt ccatgcagga cagtgatgga gatttctata attttgtctt cgaggatggt
    61561 agcatcaaca gaaacggtcc gacatcaaga aaaggaggca actggtgggc ggcgagagct
    61621 ctgtatgctc ttgcgaaagg ggctcgggtt tctcagaatc tcgatccaga gttctcgatg
    61681 cgcttggcag atgcggccca acgttgtttt cgaaaaatca tcggttttga gcgtgacggt
    61741 cttatccagg gatacactga tctttcctca gtagttcttc ttggagcggc ggagttgtat
    61801 tccatgagca acgatgcgct attgggaggt ttcataaaga gatgcgcagc tgctttgcag
    61861 agcaaagtca ttcagaatga ggactctatt ttcaatggtc ttgtcgacga atcatcgcct
    61921 tcagaaccga gatacttctg gcacgggtgg ggttcaagac agctggaggc gttggcagtg
    61981 gcgggagaga ttctgggaga cgacagcctt cttgaaatct cgacagaatc attcaagcaa
    62041 agctctttgc tccttataaa cgcggggccc ctttactcaa tttcgagcta tttacagctt
    62101 ttcccacaga tcgcctatgc agctgaatcg gcaatttcag cgggcttcag actatatcac
    62161 ttaaccggcg atagggatat tgcagccata acggctcttc ttgggagttg gttccttggg
    62221 gtcaacaaat tgaatcagtc aatggtagga aagaacgggg agggatttga cggacttgag
    62281 ttctcacatg tgaacaaaaa tgcgggagcg gaatcaacaa tttccatgct tctttctctg
    62341 gaaagaatca agagacttcc cgaagagtac ggagatctgt tctcagcagc gatagaggtt
    62401 ctcaccccgg tagtggtttt ggaagctgaa tctatgaggg cgggcctttc agaatcggag
    62461 attattcaga gtactggagt ctcaggaagt gcggtgacca aaatttccgg agcatttagc
    62521 ttacgtcaac cagtcaccct cgtagaggct tcctattcag tctttgcagc tataaatgaa
    62581 gcaaaaacga atgaagtggt tttcaccttg agaatgggag aatcaaaatc ggaaaagacg
    62641 ataagaatcg aagagaaaac agtgcagaag ctaggagtcc tcaagggaaa tggaaaagag
    62701 acaacgctta cggtcggagg aaaagtgagc gaaggaagct ttgacatcga tcagatcatt
    62761 ctagtgccag acatcgttgc agtttatctc gttgaagatg acgtaacgat tgtcttcaat
    62821 ttcaaagggt gcagtgaatt tgacgaggga gtatccgtaa tcgatggcag ggtgtttgag
    62881 agagaaaaga gcgttttaca agtaccggtg cttccagtaa gccttattga gatgaatggt
    62941 tttgtccacc tagatctgct caagtatttt gataacaacg gaattgctac ctctgacgaa
    63001 aggaaaacag caaacttcga taatcctcag ggacaattcg gagcttccta catatcctcg
    63061 gagcttgaga aagctatcga ttccggtttt cttgctgtag aagacaccga attcatggtt
    63121 aatgtagacg gaaacgacaa cttcaggctt acaggacagg cgttgacatt ttcggcagaa
    63181 atgttcgata agatctgcat tcttggtgcg gcgaatcacg gtgattacac aggtgaagct
    63241 cttctaattt atgaagacga ctatcagcaa gtgattccat tgtctttttc cgactggtgt
    63301 ggaggtccct ctacaggaga gaagatcgca ttcgagtttt caggtagata tgataataca
    63361 ggcaacacgg agagaataaa atgcatgttg tattactgtt gtatcaatct tcttgagaag
    63421 cctttgaagg gaatagtatt acctcaaatg cctaatgtac atgttttcgc aatttctctt
    63481 tctagagctg aacccggttc tcaggaagag taactgcgga ttttagaaca taaattttga
    63541 tgtggaagac ccattgcatg ataagaacat atgcccttta gaaaactaga taccaaaatg
    63601 aattcgggat gacactgaac gagatcccaa aagagaagtc tcaagcagga gagctttggg
    63661 gcagactcca cgggatgaca gcgaataatt attcgaaaat tcgctgaggg ttgaatatac
    63721 agagatcttt caaaggccac tgtaggggtt acatcctcag tagcactgaa agccgtgaat
    63781 gggtcaactg ctttcgttat cgagacccca gaccctatac tctataccct gctcctaatt
    63841 ccatg
//
LOCUS       DAOQYI010000009        58409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_565_length_58409_cov_73.224852, whole genome shotgun sequence.
ACCESSION   DAOQYI010000009 DAOQYI010000000
VERSION     DAOQYI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 58409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58409
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_565_length_58409_cov_73.224852"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKL93_04410"
     CDS             complement(<1..461)
                     /locus_tag="PKL93_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730689.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ70537.1"
                     /translation="MSRAMSKITLGIMVAGLLWFSYSSVSAVYPFNWSSLTKYYKLFL
                     EGILTTFQLSVIALALAVIVGIIVALARDSKINLLRDAGTLYVWFFRNMPLLVVILLV
                     YYGFGSAVNVDRFWAGVISLSLFEGAYVGEIFRAGIDAVPSGEKEAGEAMGL"
     gene            complement(472..1239)
                     /locus_tag="PKL93_04415"
     CDS             complement(472..1239)
                     /locus_tag="PKL93_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNQ70538.1"
                     /translation="MKKLLLVLVLLCMTLAFGSLIDDVKQRGVLRVGQDAGYMPLYGT
                     NPDGDRIGLEVDILKKMAEILDVKLEFVVVNWDGIIPALVSNKFDIIWSAMTITPERA
                     LRVNFTDPYLTVGQIILYNTNKYELPPTEEDLNDPDVKIAVQLGSTGAEAANRLLSEA
                     QIFMFETIDEAAFQVASGRADAMIFDSIYARFMAKKYDQLEVTEDLLTREDFGVAIPK
                     GDFETLQWLNTFIQWIKTTGTLAELENYWFVEYEPEF"
     gene            complement(1500..2111)
                     /locus_tag="PKL93_04420"
     CDS             complement(1500..2111)
                     /locus_tag="PKL93_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNQ70539.1"
                     /translation="MKVGRIIVLAGNVGAGKSTIASAIAEGLGFNIHYESVSDNPFLE
                     DFYYDQKRWSYHLQTYFLYHRYCSLKSAEVNENTVFDRSIYEDKEIFARNLFETGKMS
                     DREFKAYVTMFDSMIKYLKKPDLLVYIDADVDTILARIRRRGRQMETAVPIAYWEQLD
                     RLYSSWIRDYDLSPIYKIDARSVDIVINPEKAQQITKEIMRIL"
     gene            2217..3788
                     /locus_tag="PKL93_04425"
     CDS             2217..3788
                     /locus_tag="PKL93_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ70540.1"
                     /translation="MKSRHRVPRIATQLLFLLLVLYISVGHYLSEKGIAELAGTTSLH
                     AVCPFGGVVTLYNLVSDGTYVQKIHPSNLFVLGGLLISLVIAGAFFCGWICPLGSVQE
                     WIGKAGRKLLRSHYNKVPAGLNKILSLGKYAMLIFVIVQTARIGTLSFSSFDPYYNLF
                     NIWTDEIALSGYIAVFLTLGASLFIERPFCRYACPLGAINGLFNRFSIFRIRRQSSTC
                     IDCGLCDKVCPAGINVSKVSSVNSPACNRCMKCVDICPANSNSTLTIEVGLSKKSSKA
                     SKTVSVKTYIILALSAFLVPVGLSVLTGKFETEKTRVYIVPDDIKGSTSFEEIVENYP
                     ISKEQLFNGLGIPEMVSVELKVKDLTGILGFIGEEEVISREHLSALIGKLGEPISSLA
                     EHSKVDFENIIEFVQSSGLDESTVVSEVMASGPPGLFTYLFSGRWPFETSGDGAVIEE
                     KETGSENHGQSASVDIKGSTSLLEIERMVPDFEAFLEYFGLPIDEPKSSLLKDLKTKY
                     QIEISDIKDYVSNNQ"
     gene            3881..4339
                     /locus_tag="PKL93_04430"
     CDS             3881..4339
                     /locus_tag="PKL93_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70541.1"
                     /translation="MKKLLLILVVFGIAVAVFSEPLLVWPDKTHGKPLVAGLYSPVYG
                     EAKLDVFGNITGWTGPNLGLGWTWKTYFSPLELQKVNFYYEFGTNVVIFPYIGVGFDY
                     ALVLQNNQTLLVGAGASASPFTVLGFFFEGPAAILSSVLSSVILSVAVVF"
     gene            4381..4815
                     /locus_tag="PKL93_04435"
     CDS             4381..4815
                     /locus_tag="PKL93_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70542.1"
                     /translation="MSELENSGRERDQAEKKVDSEVKSVLTKYTFVAVLVLAVVLMLL
                     SSRGRVEEFDPESVFPTIEETSLLFINENLLPEGEEIELPEIVSIDYSEIEWGLYSYE
                     AIVKYYLSGAVRSLHTVWEYRVRDDTLDLLGYSSDGENWLEP"
     gene            4945..5313
                     /locus_tag="PKL93_04440"
     CDS             4945..5313
                     /locus_tag="PKL93_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70543.1"
                     /translation="MKKVLILVAVLLVASVFVAAPKNFILGQGLYGDVVYDLAGNITG
                     YEGMDFYIFQASTYYEKALTPGELNAYTFDGKFLFFIPVFGRGWEFVLRPVDNIILTA
                     GLSLLYNFLPGPYLGLTILL"
     gene            5593..6834
                     /locus_tag="PKL93_04445"
     CDS             5593..6834
                     /locus_tag="PKL93_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNQ70544.1"
                     /translation="MRELKKEAKYIVERSIETVQPESSVKNALEKLRLKGPIFLLAVG
                     KAAWRMTKAAMDKLGEEVSAGIVITKYGYSQGDIERVEIFEAGHPILDRKTVEATDYA
                     LKKIEELKPEKILFLISGGGSALFEKPLEGVMLEDLVDVNNRLFRSGASIVEINTVRK
                     HLSSVKGGRFAKLVSPSQVYTLVLSDVLGDRLDSIASGPAYPDSSTSDQALAVLEKYA
                     IDVDSRIIEALMVETPDRLFNVQTEIIGSVARVCESAATFAREIGYNATILTTSLDCE
                     ARDAGVFLASIAREEAHNRPVEKPAALIIGGETVVHVRGRGLGGRCQELALAFSLGIG
                     EMGGVTLACVGTDGTDGPTDAAGAIVDGRTVRKMLDAGIDPQLFLLDNDSYHALEKSG
                     DLFITGPTGTNVNDLIVLLCE"
     gene            complement(6909..7742)
                     /locus_tag="PKL93_04450"
     CDS             complement(6909..7742)
                     /locus_tag="PKL93_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70545.1"
                     /translation="MRKSRREIVLKTVIAWIIALIWILPLMGVVMTAIRPFSEVLDGW
                     WSLKPFTATLSNFTGALNHRTAPLYRGMINSLLIALPSTIIPLMLATLAGYGISRYGF
                     PFRKTFILTVVLTLALPQQMIAIPIFKIMSTLGLVDTYFGLILLHTAWGIPWITFFMR
                     NYFKTLPTDMEEAARIDGAGDLGIFFRIVLPNAFPAIASASALQFTWVWSDFFLALIL
                     IYSPDKLLATQRIPLMRGVYHVDWGLLSAASLMVMIVPILIYVLLQRYYVKGMVGWTI
                     K"
     gene            complement(7739..8623)
                     /locus_tag="PKL93_04455"
     CDS             complement(7739..8623)
                     /locus_tag="PKL93_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70546.1"
                     /translation="MALRKAKFRHFSYFILPALLLLVIFVLYPTFNTIFLSFVDSEGN
                     LSLSNYREVFSSRDILDPRGLERGFPFGSLIHNLMWIAIHLPLTTLVGLFLAILLRDV
                     KGGGIVKSIIFLGMVMPMIVGGIMIRFMFEENVGVVNLVLQTLGLEGKTWTAYPETAL
                     FALIFGSVWLWTGFSMILYSAGLETIPNTYYEAAQIDGASKFKMLLKITIPLLKPITV
                     VVLTMTLLWELKVFDIVYIATMGGPGGSSNVLALQMYLYGFREWDFGKSAVVAVLITA
                     STLLAAIPMMKSAGDEAE"
     gene            complement(8682..9845)
                     /locus_tag="PKL93_04460"
     CDS             complement(8682..9845)
                     /locus_tag="PKL93_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70547.1"
                     /translation="MKKLLVTVLVVLSTALISSTVSVIGPWSGVEMDAFLPVLEEFKA
                     KTGIDYTYQTYRAEDLANVLPAQFSAKKAPADVIFMWSNFITSNTQHVVDLSDVIDTD
                     AYIPGALDNVKTSDGRVFGISYTAKVKPGFWYRKSFFESHGLKAPTTWDEFVALLEKI
                     ETIPGVKDAIASGNGVGWPLSDVTEHFLITFGGPELQKDLIEGNTTWRSYAVRNAMEK
                     LVYLLEKGYFSEPTEWTTILEQWWNGDYGLYFMGQWITGMVDDPNDLAVFSLPGNRGM
                     VFSIDYAFVPEYATNKAEALELVKFLSSVEGQSIQVAQGGHIATVGVDMSNYPAVDKE
                     IAKLTEGVETLNDLDDSIGGLWQTAFWDQLKLIWVRPERLDDALMDLDEKMPK"
     gene            complement(10314..10517)
                     /locus_tag="PKL93_04465"
     CDS             complement(10314..10517)
                     /locus_tag="PKL93_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70548.1"
                     /translation="MEDGYCINYHECPVVRAYENGKINREWIEMYCEKDWTECARFRL
                     DDLLSVSTDFVLPDGSIEERLSK"
     gene            10665..12236
                     /locus_tag="PKL93_04470"
     CDS             10665..12236
                     /locus_tag="PKL93_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70549.1"
                     /translation="MHRRLSSLLLILCLVLVIALSGCVFRRYSVVVSCTPLIEKIEVG
                     NRERELPYSFSARKGQNIVVKAPLVPGYDFEGWLINNELRQTAEIAVEIEENTTIKAI
                     YSPRLVHEITYGGSGYENGTGLVVENSNEILIGITSSSTDSSLILENKGKSDFWIPFL
                     QYESNNYVVEPQLCYGGSDEESLMDTVRIAGGYVSAGWSDSSDIAYSGSGYPYHGGTD
                     GYVIMMHNDGSLRWQTYIGGSGDDEIRSVNTTIGEKLIITGITGSSDSNFANSGYDGS
                     KDGWVALMSSSGTLITDLIKVFGGSAEDGLLYAMQTKEGGYIVCGYSSSSDGDLNSLS
                     RSTGEDLWVLKLDSQLEIEWQDLFGGQNDERANVIKEVSDGYIAVGFTESAGGDINEN
                     KGGKDLWVLKFSSDGDLEWSKTYGGSEDDEGFDVVQTADGGFAICGYTDSSDGDILHE
                     NRGMSDVWVIKLGYDGYLEWEATFGGSENDFARRINETEKRELIVIGTTSSSDRDIKR
                     PIQGPSDAWLLRLGR"
     gene            12252..12926
                     /locus_tag="PKL93_04475"
     CDS             12252..12926
                     /locus_tag="PKL93_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HNQ70550.1"
                     /translation="MLLKADNVLESEIISLLEEGELCPVCRSMEKSVDGWIVGAFSNL
                     LHNEGARMELRRNGLCTVHLKRIVEVAKERSEVGSLAVSIMFKEILAAQVSWLDEKDV
                     GFLSRKKPKSGSCVLCSVASNSERIYLSAFVKFLQRDEVQRSYENSRSVFCVNHSRYI
                     IKNMRNSCGQWLAGTQRNKYEDLTAEMENYISKHDYRNTTPVGQERHAWLNASKLIGE
                     RDAKNS"
     gene            13637..14827
                     /locus_tag="PKL93_04480"
     CDS             13637..14827
                     /locus_tag="PKL93_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNQ70551.1"
                     /translation="MSELFNKEEFLKGLIRRLHEEPDKLENIREDFMRVVRELTPVEI
                     AQVEQKLVEEGMPPESIQLMCNIHLDVFKEALDEDSIDVGSWHPLHILIEEHRDILNR
                     TKELRDLTGKIISGNYDGEDIRRLQSSIQYLDSLEVYFSKEENVLFPYLERHGLVQPP
                     AIMWKEHDEVRNLRKKMKIEYENLKNGDPNKVNELAITISELFTNHIYKEHKILFPSA
                     LKLLSNNEWESIRVEFDEIGYFSFEPMPFVSSEEVSKNNGVEGLVNLGSGFLKVDQLL
                     LMLNSLPFDITFVDENDTVRYFSEGKERIFVRTRAIIGRKVQNCHPQKSAHVVTRILE
                     DFKNGVRDVAEFWLNLGPKIVHIRYFALRDRSGKYAGTLEVTQEISSIKALEGEKRIY
                     DPLD"
     gene            14919..16046
                     /locus_tag="PKL93_04485"
     CDS             14919..16046
                     /locus_tag="PKL93_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ70552.1"
                     /translation="MIRREMGKTGEKVSALGFGCMRLPTCDDQSIDVDEATRMLRFAI
                     DNGVDYVDTAWGYHNGQSEPFVGSVLQDGYRDKVNLATKLPSWLIKCREDMDYYLNEQ
                     LRRLRTDVIDFYLIHALNRRYWKNLKEHDIFEFMDSALASGRIRHIGFSFHDTLDVFK
                     EIIDSYGWQFCQIQYNFVDTEHQAGLEGLKYAAGKGIGVAVMEPLRGGKLATNVRDDI
                     LSIWTSSGIDRTPAAWALRWVWNDPDVGVVLSGMSTMDQVRENIKTAREASPNFMSSS
                     ELAIVDRVREEYKKRIKVSCTGCSYCMPCPSGVAIPTCFDFFNDAYMFDSIEDHKKAY
                     LRSVKKENRASLCVECGRCEELCPQNIPIIEQLKEVSSLFE"
     gene            16159..17508
                     /gene="glmU"
                     /locus_tag="PKL93_04490"
     CDS             16159..17508
                     /gene="glmU"
                     /locus_tag="PKL93_04490"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNQ70553.1"
                     /translation="MTGIVLAAGQGKRMKSRFPKVVHKILDRPMVNWVISALRQAGSD
                     RIVVVTGFEAAMVEAVLDKDIISVRQTPQLGTGHAVMTALDYLDDEDIVVIAGDEPLV
                     LPSTLKKLVSRRIEGDFDVVFLTMKPADPTGYGRVVKDGKKIKIVEHRDADSETRMID
                     EVNSAIYAFKGSFLSSAIKSLSNNNDQNEYYLTDTISLADKADTIVVENAQEVLGVND
                     RIQLAEINKIAQKRINVEHMRNGVTIVDPDTCYIGPEVEIGADTVIEPMVFLSGKTRI
                     GNCCSIGPLTRIDSSVIGDDVEILRSEVSNAEVHSGARVGPFSRLRPGAVVMNEAHVG
                     NFVELKKTILGRGSKAQHLTYLGDTDVGEGVNIGAGTITCNYDGKRKHRTEIGDGAFI
                     GSNTSLVAPVRIGKNSVTAAGSAITEDVPPDSLAFGRARQVVKEGKYSKNRGQEENS"
     gene            17508..18455
                     /locus_tag="PKL93_04495"
     CDS             17508..18455
                     /locus_tag="PKL93_04495"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNQ70554.1"
                     /translation="MPYQTNEMKVFAGSASLPLAEKISNYLGIRLGDCRTKRFADGEI
                     NLKIDETVRGHDVYIIQSTCSPANENLMELLIMIDAFRRASAHSISAVIPYYGYARQD
                     RKARGRDPITAKLVANLLTTSGASRLITIDLHAEQIQGFFDIPVDNLWSFPAFLKFFS
                     QSAFDRNEMAVISPDIGGVKRASKFAAKLGVPLAILDKRRPEDNVAEMVHIIGDVEGK
                     TAIIFDDIIDTGRSLVEAAKMLKSNGADKIFACATHGVFSGDAKKIIAESTIEKVFIT
                     DTIYHDDLPDNIMMLSIAPLLAEALTRVRKNLSVSILFR"
     gene            18469..19113
                     /locus_tag="PKL93_04500"
     CDS             18469..19113
                     /locus_tag="PKL93_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNQ70555.1"
                     /translation="MHEINLVAELRNGEMKAKSLLNQGKIPAVVYGPELETVPVSLDK
                     VEILRIMNRIAETTSVVISIDGKEYHTFLKGVQRDKVTDSVIHLDFYVPSSGHKMELR
                     IPIRLKGTPIGVEKGGIVDHIINELPVSVLPKDIVEEIIVDVSSFDVGHVLRVGDLEI
                     PEGIKPLLDDEDAIMLIALPRAVKEVEEEEAEEIEEGVEPEVINKGKKEEEEEE"
     gene            19116..19685
                     /gene="pth"
                     /locus_tag="PKL93_04505"
     CDS             19116..19685
                     /gene="pth"
                     /locus_tag="PKL93_04505"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNQ70556.1"
                     /translation="MFLFVGLGNPGPRYALTRHNVGFLFIDQMAKLGLRKTVQKRLYE
                     AHLLEGEEKVYIAKPLTFMNLSGVAVRLLMKDLNFKREDTLVLVYDDVWIPLGRIRIR
                     ESGSDGGHNGLKSVISEIGTQSFPRIRIGVGPLPENGDMVDYVLGRFSNDEYQKVSKV
                     IDLAVSAAKELISGDIRKVMTAYNGTEVD"
     gene            complement(20264..22270)
                     /gene="uvrB"
                     /locus_tag="PKL93_04510"
     CDS             complement(20264..22270)
                     /gene="uvrB"
                     /locus_tag="PKL93_04510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNQ70557.1"
                     /translation="MLFEMDSSYSPQGDQPQAIEKLIDGLKSGDRFQTLLGVTGSGKT
                     FTMANVISTLQRPAIVISPNKTLVAQLYREFKTFFPKNKVELFISYYDYYMPESYIPS
                     KDLFIEKEAEINETLERMRISALKSVLTRNDTVVLASVSAIYASGDPKDFATMNIFLE
                     RGKSINRKELALKLANIQYNRSEDVSAGGVFHVKGEILEIFPPYEDFGIRLYFFDDEI
                     ERIVTFDPINRKTIEELDMVTVYPAKEFVTTQDKILGAMRNIENELEERVKQLEREGK
                     YLEAQRLRQRTTYDMEMLSSVGYCSGIENYSRFFDGRMPGEKPYTLLDYFDREKFITF
                     IDESHIGVPQLGAMYRGDYSRKKNLVDYGFRLPAAFDNRPLKFEEFLSTVGQIVFVSA
                     TPGNFEAEHSTRIIDQVIRPTGLVDPEVIVHPTESQVDDFIERMREVKKRNERALVTV
                     LTKKAAEMLSDYLNEVGIRSEYLHSELDAIERVEVLRKLRDGTVEVVVGVNLLREGLD
                     LPEVSLVAIMDADKEGFLRSETTLIQTIGRAARNVNGQVILYADTMTGSMKRAIDETN
                     RRRLKQLIYNKEHNITPESIVKPLFRNIFEEFAGENLDKEREEKARATYLEGVMALKE
                     SLDYEDYIALLNEEMIRAAGELRFEDAAILRDEMYSLKKSKQNI"
     gene            complement(22557..23150)
                     /gene="ruvA"
                     /locus_tag="PKL93_04515"
     CDS             complement(22557..23150)
                     /gene="ruvA"
                     /locus_tag="PKL93_04515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNQ70558.1"
                     /translation="MLEGIEGRVREIRETEIVIQVGSLFLKVNCTPNTISKLPVEKDV
                     YLLTFMAYSQDRPPELYGFYDQEEKELFNILLRASKIGPKSAIKILSSATPSRIKHII
                     STKNAAELSSLPGIGKKTAERMIVELASLVEASGITDEETIPSVERSHGEEAVAALTS
                     LGFDENKSRKVVIEILKTEREIDTHDLIKKALKEIRK"
     gene            complement(23134..24453)
                     /locus_tag="PKL93_04520"
     CDS             complement(23134..24453)
                     /locus_tag="PKL93_04520"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNQ70559.1"
                     /translation="MKTYSEAIQYLYNSRPYGKIKYGLFRIRELLERLGNPQKSYPII
                     HITGTNGKGSVAAITRSIMSSHGLKTGLNISPHITTFRERIQVDGEYISEDDVCRILK
                     NIEPVLEKMDRKGEEYAPSFFEVVTAIAFQYFKEESCDVVVLEVGLGGRFDASNVIDS
                     SLVSVVTSVGLDHTGILGDTEEKIAIEKSGIIKLECPVVSGITRPAIRRVVSEKARSM
                     NSPYFFYKRDFDATGREYALNRNMFDYTGSNNFENLQINLNGEHQTVNAALAIKTSEI
                     AMGEIRSGLDEEKLRMALKSVSWPGRFEVFSQDSKTVILDGAHNPDAARVLRRTVEKY
                     FPGERLTLLFGSLDDKDYETNIKVLSEITDSVVVCRVPNHRSIHPEKVADVWRKYCSS
                     VQFVEEHEIALNKALEISDSLLVCGSLYLVSEIRNLLTGVSEHAGRY"
     gene            complement(24450..27053)
                     /locus_tag="PKL93_04525"
     CDS             complement(24450..27053)
                     /locus_tag="PKL93_04525"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNQ70560.1"
                     /translation="MKELSTRYNPSDLEEKWYSCWKEGGFFEPKGSGEPFTIMIPPPN
                     ITAPLHMGHALNLVIQDIIIRFKRMKGYRALWVPGEDHAGIATQNAVEKDLAKKGTNR
                     KELGRERFLETTWNWAKEYRQRIREQIEAMGCSVDWSRERFTMDEGLSEAVRKAFVML
                     YKEGLIYRGKYIVNWCPRCSTVLSDEEVEHEDEKGAFYYIKYPIKDSDEYVIIATTRP
                     ETMLADTAVAVYPSDDRYEGLAGKTVILPLMNREIPIIQDRYVDPSFGTGALKVTPAH
                     DPNDFQIGLRHGLEVIEVLDKDARINENGGKYKGLDRYEARKQIVKDLEEQGFLIKVE
                     PMVHAVGRCYRCETVVEPSLSDQWYVKVGPLAEKAIEAVEEGSVTFFPETWKKVYLNW
                     MNEIRDWCISRQLWWGHRVPVWYCDDCNEVIVEEQDPSVCPKCGSKNIRQDEDVLDTW
                     FSSQLWPFTTLGWPEKTEDLESFYPTSVLVTAFDIIFFWVARMIMAGYHFMGEKPFNH
                     VYITRLIRDKNGRKMSKSLGNGIDPLDVIREHGTDAMRFTLAILAAQNHDIKLDVRFF
                     DIYKKFANKIWNAARFALLNMEGFEKVNLDEEELAIEDRWILSRLAKSVKAVEESIDG
                     YDFPSASKMIYSFFWNEFCDWYIESIKPRLNREGRGRIVAQNVLVRTLDASLRMLHPF
                     MPYITEELWQNLPGSDGLLITARWPEFDESSFDNPSEMEYSRIMDMVRGVRNIKAEMN
                     IPPSSKTDVYYIGEVLNQEVVELVSHLSNSNEIDRRAEKTSGSVSAWGDAENTLYVVL
                     GEIDTDAEIERLTGKLEKERGNLTRTLSKLENGDFIRNAPEEVIAENRERLATCKDNI
                     RRIEKIIEDLK"
     gene            27164..27520
                     /locus_tag="PKL93_04530"
     CDS             27164..27520
                     /locus_tag="PKL93_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNQ70561.1"
                     /translation="MIRVSYMVEEDLSTYKKIYKMVRMIPVGKVATYGQIASLVGKCS
                     ARMVGYAMAGISDETIPWQRVINSRGRISIRDPNGYTLQKAILEREGVHFDESDTIDL
                     SVFGWEGPEAESAKDW"
     gene            complement(27612..28751)
                     /locus_tag="PKL93_04535"
     CDS             complement(27612..28751)
                     /locus_tag="PKL93_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70562.1"
                     /translation="MLVLFREGTASAPGPIFHLSHEALGIEADQVLIVQPILNQTSYV
                     PTEERYEAQFIKDSLSTVVRFTKLPYCLLISRADALYTAQHLPGKFLLEGDLLYHIGP
                     NWIPSHSAVYLGVDYETAMTDEKLGFNNEVTFGESYPFLSHGYNLVEEGVFIFPNDIE
                     NHSYVNLFNYDTYESRWLRYWATSFSGARRFPGEISPQDRRKISRFMYETAEKGALWS
                     VGFAWSGFWDLPFTDRDLYSCVGVVEKAYESAGRNIVPFWEDMFYLNSFEQFSRTIPV
                     PEITSMVGDKVEFRINSLLAEWTYVGFDKWSFGDHAWIWKSESNVELLSPQGSLEVKN
                     DHCIFTWTPLETGDYPIIFRFFGEFEGKEVEKYHTLTVHVTGKDS"
     gene            29053..30549
                     /locus_tag="PKL93_04540"
     CDS             29053..30549
                     /locus_tag="PKL93_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNQ70563.1"
                     /translation="MKRLPRSTSVAAGTIAILTFSILSKGMGFFREMLVAGLFGTSAN
                     LDAVFVAMTPATTLSGIIAGALAAIFVPVYHSIRNEDTERSKRYAGAVLISGSLVFLS
                     MGIVFLLIPDLVIRLFAPGFSSEIVAYASRKLRYLSIYPLIGGLESILGAVLKSNRRF
                     VQYGMSQLFFNVIAIPVILLTSPFLSEASYILAWMLGNAITVLLYLIQSRDLFTLRVG
                     KGTSIVETLYLSLPLIFSGSLGVINNMVDKAFVSLLPPGRVASLQYAHTLLGLITFMI
                     SAFQMTAYTELSELIVSGDRERVEERLRKTVTTSLNISLPLAAWIIMMAEPLVRVIYQ
                     RGEFDAGSTNLVSMALIGYGALIVLSPIAHTCSSYFTARKKLKSITLVAVFSIFLNAL
                     FDWLMLEPFGHAGIAASTSLVVLNATLIYVLLIAREGLRFMPLKRIMRLLGSSVAMYL
                     IVLLLRNNVSTWIWLLVGNGLFLCLFLVSAKSEMKAMMSRISFLFKRT"
     gene            30623..31858
                     /locus_tag="PKL93_04545"
     CDS             30623..31858
                     /locus_tag="PKL93_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ70564.1"
                     /translation="MREVVILGAGPAGIFAAINCTTAGRNVTIIEKTSSAGKKLLISG
                     GGQCNLTNAESPSLMLKRYFGAGRFLKPSLMAFDSQKLMDFFERRGLRLHTREDGKVF
                     PVTYSAGDVLEVLLKECDRQGIKIVYNCRAKDVIQFPSGGFLVKTESDSINADYLLIA
                     TGGKSYPETGSTGDGFALAKKLGHSLSEQRPALTSVTIRNFGFGDLSGISLRNVEVSL
                     WRNLKKVTTRVEDLLFTHDGFSGPVILHLSREASPGDIIRLNLSGQLREKLEANLISL
                     LSKEGKKLVKNVLDEYGLPERLLSKIIDLAGVGERICSEVRKDERKALVTGLCELDFE
                     IERVGDFTNAMVTRGGVSLSEVNPKTMESRIVNNLFFAGEVLDFDGETGGYNLQAAFS
                     TGYVAGRTINARTSRVENR"
     gene            32045..32233
                     /locus_tag="PKL93_04550"
     CDS             32045..32233
                     /locus_tag="PKL93_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70565.1"
                     /translation="MEVKKPKWFDSWIEIDESYLKVAGTSWRLKANAPKKVKREYQRL
                     LKQITGNNDYPYSWKHER"
     gene            32300..32830
                     /locus_tag="PKL93_04555"
     CDS             32300..32830
                     /locus_tag="PKL93_04555"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNQ70566.1"
                     /translation="MSITTGTGDKGETSLWSGERVNKDSLRVEAYGTVDELSSFLGEA
                     KHFVSEEIGRHIVSIQRFLFKLAGQLASRDMQYVEPIVENDIVAITELVHEFESVVKL
                     DGFVIPGSTIGSSKIDICRTVARRAERRIVALSRVETIDTTLLKYINRLSDLLFIFAR
                     YEEYKAGKIVYKKGIK"
     gene            complement(32833..33111)
                     /locus_tag="PKL93_04560"
     CDS             complement(32833..33111)
                     /locus_tag="PKL93_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70567.1"
                     /translation="MELKSNLEDAYSVVTVRDFGKAWRRRTARIQLKKSVVSEAELQK
                     ITRKLWETSGQDVDEMITVFYLPGMDTSSVAYGFGSCMKDGIARVSYR"
     gene            complement(33661..35037)
                     /locus_tag="PKL93_04565"
     CDS             complement(33661..35037)
                     /locus_tag="PKL93_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HNQ70568.1"
                     /translation="MAFQIRGVVEGFYGKPWTMDIRREVIQYLGDHGFNTYIYAPKDD
                     ELHRKRWREQYDENFFKEFSILVQEAESRGISIVFAVSPGLNIAYSSNSDVNTMVEKL
                     MRLADAGVRSFGLFYDDIPETLIYEKDRNTFSSLSSAQAYFTNSVFKSINEKLKTLSR
                     FMICPTQYCGREATEYMKTLGRELDRSISILWTGPDVCSNVLSADNAELAENAFQRKI
                     LLWDNYPVNDASMVPELHVGPYEGRDPNIVNHAEGIVLNPMIQPLASKIAIFSASEFL
                     LDPYHYDSDSSWRRAISEIAPGCEEEMIHFCEYNLRSPIHTTHSERLTGLTSNLSKLL
                     SKNRWPEVYEILLGEATKISESEKALNERLPADLIREIEPWIFEFSLWGDALTKTTGI
                     LAMRNLMFSSSVSYEELSETTKLCREIEISLCKLVKTETLTGGILFREIVQEILVRTK
                     GYLKLIIS"
     gene            35223..36059
                     /locus_tag="PKL93_04570"
     CDS             35223..36059
                     /locus_tag="PKL93_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNQ70569.1"
                     /translation="MKIGLVTDNTCNLTAEEIKEYDVRIVSLYINRNGNYTKATELNL
                     DEYYEELAAAAELPRTSQPSPQDFIAVFKEALEIYDALIVPVLSGKLSGTSVSANIAA
                     KAFEKPIHVIDSQLIADGTGMLVKNLGDMINSGANLDELVEYGTNFHEKTRTFCSTDD
                     LSYLVKGGRIGKAKALMGNLLKMKPVIVVDEGELKPVENVRGNKKLLDVLVSHVFSEI
                     PPERLRRARVLTIWRKEDGKTLERMLREKAPEAEVESRVIEAIIGTHLGPQGIGVISE
                     MK"
     gene            36126..36353
                     /locus_tag="PKL93_04575"
     CDS             36126..36353
                     /locus_tag="PKL93_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70570.1"
                     /translation="MTGGKRVLDALERIEIRYGKVFDFLQSMYRLTNEERLTKRFSEL
                     SPGYAPVGKIDEWVKSSRANQRTGIILSIDF"
     gene            36621..37724
                     /locus_tag="PKL93_04580"
     CDS             36621..37724
                     /locus_tag="PKL93_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ70571.1"
                     /translation="MEYRRLGKTDLEVSLLGLGGFHLLEIELETVKEIVERYLEAGGN
                     YFETARSYGDGVSERKLSLVLPKDGIIVASKTEGRDRKSSERDLLATLKALGREHIDI
                     LFLHAVTKNSDWEEISSSDGALRTIEWAKREGLISYVGITSHGYGGTLLRALKEYPFD
                     LFMTQMNYYDRFNFPEIETKVIPFAVSNNIGIVAMKPLADGYLWRSTENAFRYTLTIP
                     VSCIVSGINSIEQLEEDLRLIEKEPFSSEELDELLLTAPELGNYVCRQCMKCLPCPQG
                     INIPGFFLAEGRYDRQMLDGKVRDAADYALRDRLAHWFGSEEQGLSEYSTISPGLDSC
                     DDCGVCNERCPYEIDIPRKLRIVKSKFTEGYIW"
     gene            37740..38270
                     /locus_tag="PKL93_04585"
     CDS             37740..38270
                     /locus_tag="PKL93_04585"
                     /inference="COORDINATES: protein motif:HMM:NF039476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HNQ70572.1"
                     /translation="MRKTSFPSCEVVGFSFVFIVGTLLHMAYDWSGDDAIVGIFAPVN
                     ESIRELLKMVFTPGLILLFLELLFCSEIPTASFLSGKAVGIYVMCVTILGGLYSYSLV
                     IHYGLILDVLLMVIAIALGQLVSFKISPRIAADSTAGIISLVMLVLLGASFVVFTFLP
                     PHWEFFRDSITGAFGI"
     gene            38339..40375
                     /locus_tag="PKL93_04590"
     CDS             38339..40375
                     /locus_tag="PKL93_04590"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNQ70573.1"
                     /translation="MLSSEEIFKSMEIKLEDTLPPDPEFKPGIRRAPKRELTLSKKEI
                     ELALVNALRYIPVRLHEKLAPEFLNELLTMGRIYGYRYRPRGEIKGRPIEMYKGKCIE
                     GKAFQVMIDNNLDFDVALYPYELVTYGETGQVCQNWMQYRLIKRYLEELTEDQTLVVM
                     SGHPLGLFSSNKSSPRVIITNALMVGMFDNQEQWIRAQALGVANYGQMTAGGWMYIGP
                     QGIVHGTFNTILNAGRLKLGIGDEGDLGGHLFVSSGLGGMSGAQGKAVKIAGGVGIIA
                     EVDRSRIETRLEQGWVDEVIEDPSEAFALAIEKMKSRESQAIAFHGNIVDLLEYAVNN
                     DVHIELLSDQTSCHAPYDGGYCPQGLTFDERTELLRSDRKRFIELVDKSLVRHFSLIR
                     MLVHKGTYFFDYGNSFMKAVFDAGAKEIAKNGTDTSEGFIFPSYVEDIMGPMLFDFGY
                     GPFRWVCLSGKKEDLLKTDAAAMSLIDPDRRGQDRDNYIWIRDADKNRLVVGTQARIL
                     YQDAAGRRDIALKFNEMVRCGEIGPIMIGRDHHDTGGTDSPFRETSNIKDGSNIMADM
                     ATQCFAGNAARGMSMVSLHNGGGVGIGKAINGGFGLVLDGSERVDKIIRTSISWDVMS
                     GVARRAWARNENALSVASEFNRQKRGSEQITLPYLVDEGIISKAIKEAETEKEK"
     gene            40380..40940
                     /locus_tag="PKL93_04595"
     CDS             40380..40940
                     /locus_tag="PKL93_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HNQ70574.1"
                     /translation="MTMYFSKSDEETTKTLLARIFERAEEKNIDKVILPSTSGVVARM
                     AFDLVPDSVRLIIVTHSVGFREPDEDEFDPQVRKLYSGSRHSLLTVTHLFRGIEVAFA
                     KANGGVYPLQIFATALRLFGQGTKVAVEIAIMAADAGIVRTDQWVISAGGTNHGIDTA
                     FVLKPCHSTDLSIFKFGELIGIPSSL"
     gene            40994..41069
                     /locus_tag="PKL93_04600"
     tRNA            40994..41069
                     /locus_tag="PKL93_04600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41027..41029,aa:Ala,seq:cgc)
     gene            41492..43528
                     /locus_tag="PKL93_04605"
     CDS             41492..43528
                     /locus_tag="PKL93_04605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ70575.1"
                     /translation="MPSKTPERVRDGVVINDDFSASQHLISQLNEPKKLEKLSLHQSN
                     LVRILSAIRMGEVLSYEALETKLREWENDILLSTSLFRGVQRSGRKLAFRDLLTVIGK
                     SEEIDSLLSRVIRYDSSDLVAAKKHASVLLESAVLDGRDGFDPEALLDLWKENERVRS
                     LLLLLSGEKRPLIREMIGKSLGDDLSLHLGIILKAVRYGINLPDNHPALTAGPCLKKI
                     YSLLIDLRDQIRVNSVLVQFSFYGDPLKTGKGSSGGMGTFLRTLGNELTQNLPGVVTI
                     VPLEMREISRMDRLWRTEREHHFLIYVPFLGYEKMIMNNFLEERTTLISNVTDILSIA
                     GISPEQFHMRFSDFASQAMLDLSKKTGIRSFFTVTPDPQWRFVDQKGSLIKLDAVDAV
                     RETSKVEVSWKMLRDCDGLFGIGSEESRNQLLAYYPALLDESISEKLTMMPEGISLRN
                     ECPMFADGPLYSILFEEDKEFYLERSFRNNPLLLTVGRLDPAKGQVKLLKAWGNSGLW
                     NSFNLVIVGGDLKHPSRIETDEFEEIRKYVRENKELHGKFCHLPAMNNDEIRCFENSI
                     SRVKTTPWPPTYVAPSFKEEFGIAILEAMAAGFVAIAPKRGGVKSYIRDGYNGFLIDT
                     TDEVSIEKELRRLLIDARPSARRMKMIAENGSKTVYRRFSISIVAKQFSSLYTS"
     gene            43941..45455
                     /locus_tag="PKL93_04610"
     CDS             43941..45455
                     /locus_tag="PKL93_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ70576.1"
                     /translation="MRVLMVYPEYPETFWSFKHALKFVSKKAAYPPLGLMTVAAMLPK
                     EWEKELIDMNTDILRDEDIEKSDYVMISSMDIQFDSAKKVIQRCRDLGVKTIAGGPLF
                     TTRPEEFSEVDHLVLGEAEMTLAPFLLDLEKGRAKHIYKSDGFPDIGSSPIPDWSLLN
                     MRKYSSMNIQYSRGCPYNCEFCDIVLLNGHIPRTKSAEKLISEMEALYKAGWRGGVFI
                     VDDNFIGNKAKLKKEILPAIAEWMKNRNYPFVLNTEASIDLSDDDELMKLMVDASFGT
                     VFVGIETTEEESLVECGKFQNRNRDLLSSVKKMQEFGLQVQGGFIVGFDHDKPSVFRN
                     MIDFIQKSGIVTAMVGVLTAPTGTRLFLRLKKEDRIVSDFSGNNTSIMTNIVPKMGMK
                     NLVDGYYKILNSIYAPKPYRRRVITFLKNYKPNSIKHVRPFADSVKAFLKSVWVLGIR
                     DKGRMNYWSLLLWTVFFRPSLFPLSVEFAIYGYHFRKSFSFGMLVESDTQSPSA"
     gene            45618..46877
                     /locus_tag="PKL93_04615"
     CDS             45618..46877
                     /locus_tag="PKL93_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNQ70577.1"
                     /translation="MSRKSVKFGSFLRDHIDDRDVFRKWLQLGMAFENFRRRHFPDRD
                     RPRFVNTVNHLALEEVYFAVKGEKTVWVNLMNPSELLLSAGLNPVSAEGISGALSSMH
                     LEDTAISVSSQAGISDSLCTFHRASLGVSLRKLLPPPKLVMTTSILCDGNLPTFKRIA
                     REYDVPFLLIDVPRGRKQEAVGYVLSQLKDVIVTIEEITGKPFKMDELSLRLSYEREL
                     INSLVKAKLEARNSYLPQHLYEHMNSLYVLHTLAGDPRIADGSRDIVESLEVIPENAK
                     RILWLHIPPYYDNELFELFAPNGENLVVGNELMWDWIYPVDPDRPLESLAKKLVFNPL
                     CGSVVERGEFCLGLARDFNVDGVIHFSHWGCRQSSGGVPFLKRLFEGKKIPFLELTGD
                     CVDHSSQGAGQLRTRVEAFLEILENRR"
     gene            46874..48589
                     /locus_tag="PKL93_04620"
     CDS             46874..48589
                     /locus_tag="PKL93_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNQ70578.1"
                     /translation="MIYYTCSYIPLEVLMGSDIEFQRLMRSSPTSSHELGCNLCGYAK
                     TVYQKGMELDSNDCLLIVDSCDAMRRVGDLLDEHSLANVFILRLPWKRGLEAVTFLSG
                     EIQRLVSFLDSSAISIDLSEGVRRFNKLVDHVLANEKELAAGDLTDLYLHALNGKQGS
                     FTSKSGATACTSRLAITGGITDIVALDEAVKNAGGVISVNDTCLGGRPFSERTQEKPD
                     PFQAVAERLLKWRSPCGRFSEGESHSNRAVDATVFVAPKFCDFYDFVRPKDGKSVYRI
                     ELDYPINSQGQLSTRIGALMEKNSVRSVSPSKEEKKMLFAGVDSGSTTTNGVLIDREG
                     RIIYSKTLKTGVKASNTAEALIQEMTEVAFKEGNRIGKCVSTGYGRLLVSSANDRITE
                     ISCHARGVFELFPEARGIIDIGGQDSKVIRLSPDGNVDDFAMNDKCAAGTGRFLEVTA
                     SALELGIDEMALLARKRNKDISISSVCTVFAESEVVSLIGMGERIEDISAGLFRAIAK
                     RVGAMYSRLGSPVPLVFTGGVAKNSGVVDALKELFGTDIIIPEVPEIVGAFGAALIAR
                     DSVVE"
     gene            complement(49359..49718)
                     /locus_tag="PKL93_04625"
     CDS             complement(49359..49718)
                     /locus_tag="PKL93_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ70579.1"
                     /translation="MADRAIKYSANSLDFAQKVAGFSAKAVELKAPFSGRPEAHRNQH
                     DLYVVISGKAKVKTGELTGKVDEISEGEYRSDEMIVNEEHTLQIGESLLIPAGLAHTV
                     IVESGVFAQWVFKIDKR"
     gene            complement(49771..50886)
                     /locus_tag="PKL93_04630"
     CDS             complement(49771..50886)
                     /locus_tag="PKL93_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HNQ70580.1"
                     /translation="MTNETGGAKQIVVSLILLAAVFLVGIIGYSIIEGYSPIDSIFMI
                     MITISTVGYGTITELSQAGKIFTSIVILASITIVVYAVSNITAFLVEGRVNKMIRRRR
                     ILKTIEKLENHFIVIGGGDLGSTIAHEMSKRNNDVVIVEKSEERLKDLTSREGGKLIY
                     VSGDATEEETLKSAGVEKAQGLMSCLPDDVDNIFVVLTARGMNKSMKIISKVAHRENI
                     NKLNYAGADKVIPIQEIGAHRMVTMMLNPTVIGFLDTISQSGSLQLRFEEVKVPGNFR
                     PEGMTLEALKIPQRIGLIVIATVCDGVNKFNPSASTIVKPGDSLMVLGEHEQVEKLQS
                     LLGGNLNVEPQENNSFIPLILEGNYLSTPVAGEFGDL"
     gene            50970..52928
                     /locus_tag="PKL93_04635"
     CDS             50970..52928
                     /locus_tag="PKL93_04635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNQ70581.1"
                     /translation="MKKYTLKSSEIPGFLTEGLDEEQLKAVIESSGRSLIVAGPGSGK
                     TRVITYKIAHLVSNSINPQNILLVTFTRAASREMIDRARRVSGSNLKGMLSGTFHHVC
                     NYFLRKYAKAAGLAENFTILDREDAKDLIKHCRTELLEERKGINSSTLPSAGVLQSIY
                     SYSVNVLSSLRESTARKNRKFLGSYDEIEEIWKRYVQEKTVQNCVDYDDLLLKALKLF
                     NDNPSILSKEAERFRWVLVDEFQDTNVLQFRLVEMLSSVHGNLIVVGDDAQSIYSFRG
                     ARFENVYDFLSAKDARIYKIQTNYRSTPQIVELINSIVPQDSIEKQLRAVRMNGPIPV
                     VAETWDNLEEASFVAQRIQEHIDDGVDPENIAVLYRSHYHSLELQMEMDKRKMNFTLY
                     SGPRFTETAHVKDILALLKVIENPLDQVSWGRILRLFPGVGNAISLRIIQGISAAAND
                     GHRAEEVVNSHVSKRIKLDKLVSILGATNEEEPPTEVVRRFYADFYGDYLDEKFPDAR
                     ERRMDVERLIEIAGRYSSISDLLEDLAVSEKIDIERESAEKQPSVVLSTVHQAKGLEW
                     EVVFILAANPGDFPNSMAILEGSLSEEERIFYVAVTRAKDYLYILRQKGGRSRPMIGN
                     RYVFRSGHDFVEKLPKNCFERWDVSWDS"
     gene            52975..55122
                     /locus_tag="PKL93_04640"
     CDS             52975..55122
                     /locus_tag="PKL93_04640"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNQ70582.1"
                     /translation="MNSLYLPLLAGIISTIFAFVMLRRTLGFSQGNDRMKELSKYVQE
                     GASAFLEEEARKIFLVAVILAAVLGIIFQSFKYPIVLLFGALVSEAAGVIGMYAATRA
                     NARVAAGAESGLSSAFKVAFSSGSVMGLAVAGFSLTGLALVMLVFKSSFVFEGITDIS
                     KAFGRISYIDGVMIVSSYSLGASLIALFDRVGGGMYTKAADMSADLVGKIEEHIEEDD
                     PRNPATIADNVGDNVGDVAGLGADILESYVASIVSAIVLAIFMKFADHGTMTETQYYG
                     LILLPVLIAGAGVLSSLIGVLVVANLKSKDARKSLSFGNLFTGGLVLASVAIVIGIAA
                     PDYPFKDAFLINPEFVSRWRLFFAIASGLIAGVIISKLSEYYTSDDYKPTRKLAKDTQ
                     SGVAINITGGLALGMGSTLWPVITLAIAILAAYLSAGVYGIAIAALGMLSFVGYIVSV
                     DSYGPVADNAGGIAQMANLDPKVRKLTDRLDSVGNTTAAIGKGFAIGSAAFAALSLIV
                     SYIWGSAGTAEEIINNPVINLVEPYTLIGILIGGMLPFFFTSLLIKGVADTANLMIVE
                     IRRQFKETPGIRTGEAVPDYRRCIEITTKGAVSRMLTPGVVAIASPFIVGFLFGRSAV
                     AGLLVGGLSSAIMIAIFSANSGGAWDNAKKFIETGEYGGKGTPTHEAAVVGDTVGDPL
                     KDTVGPSMDILIKLMSVVSLVFGSLFPVTPFFM"
     gene            55214..56380
                     /locus_tag="PKL93_04645"
     CDS             55214..56380
                     /locus_tag="PKL93_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ70583.1"
                     /translation="MDTKKAFSLISSYGFFLFGFTSIILGSALPAIEKSFGLDHQMAG
                     VLLSLPTLAFMASAIVVSSLSNRFGPFKLLAAGIVCIATGLTVLSVGRSFWLLLVGSM
                     TVSFGTGSMETSIGIAVSGLNYKKPGGALNLMHSFFAIGSIISPLIVAAFLVDYTSWW
                     KPFLIALFVSLLLLSFIPFLYGIPFVGSDEKATHFRKEIFSRKIFWLIMVGVLLYVGY
                     EIGFTSWLSSFMFESKGIEMRYASIFPALLWAGIFVGRLLAGFFVDKLGYELSLLMMV
                     IIAFVSFGAALLFQSVFAIALAVVFAGFGFSGTFPTLQAILISSMKNGIGFAIGMFTV
                     AASIGGAMSNFLVGLLGHHFGMMAGVIFILFLIFLEMVVVLLIMRVRKERLDAQ"
     gene            56370..57476
                     /locus_tag="PKL93_04650"
     CDS             56370..57476
                     /locus_tag="PKL93_04650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ70584.1"
                     /translation="MHSKVIVVGAGPAGLAASRIFARDGIDFLLLDSKSFPREKGCGG
                     GLTPRSLKRVHEIFPEVDIEGFDCHELSINRIIDGQMKHLTTLMSKKTIFKTVDRKEF
                     DNALLKSIVERGGRFLSSRVKEIVKEEQGFVVKTEERELTSDYVIVSAGVFGAKLLGH
                     EPPSFGVAYYGYSSPTGSASVTFIPNGYLWSFPGESFDSVGGGVYPDSEKTPSYEEMS
                     TLIKRSFDEEIDLRGAPIPTFNTDVVLSLNNIYTNLLVAGDSAGLVDNWTGAGIDFAF
                     DSGRQAALSIIEDGYKGKTVNERYLERLFPAARHLQIADAFRKRFNRNFEEHLDLLKG
                     RMTGKLLSNYLSYYVKGPYKLAFKSLFTRGISSR"
     gene            complement(57477..58196)
                     /locus_tag="PKL93_04655"
     CDS             complement(57477..58196)
                     /locus_tag="PKL93_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ70585.1"
                     /translation="MVEFKDLTVTYNPGMENERVALKNVSLTILEGQFVTVVGPNGAG
                     KSTLLKVILGEVQPTSGLYMIDGKTMNYMKPHKLAEFIGRVYQDPNTGIFPALTIAEN
                     LIIASKKGNRPFKFSSVKEEAIALLKNLGLGLEDRLKTKAGELSGGQKQSLAMVMAIL
                     SKPRLLLLDEHTAALDPDSASRVMELTERINREYDITIIMITHNMDIANSYGERLMRL
                     MEGEVVNDGMRNIVSRVTSAV"
     gene            complement(58175..>58409)
                     /locus_tag="PKL93_04660"
     CDS             complement(58175..>58409)
                     /locus_tag="PKL93_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Inner-membrane translocator"
                     /protein_id="HNQ70586.1"
                     /translation="VKLFYGLLSPIVGGVIYQALLSLAMRYGYRIGFLASDMKLLTAV
                     FIITVIGLRQLSSSGGKRKVKQEVKRLWSNSKI"
BASE COUNT        16070 a        12254 c        13798 g        16287 t
ORIGIN      
        1 aggcccattg cttcacccgc ctccttttca ccggagggga ctgcatctat ccctgctctg
       61 aagatctcgc ccacgtaggc tccttcgaaa agtgaaagtg agattactcc cgcccagaat
      121 ctgtctacgt ttaccgcgga accaaatccg tagtagacaa gaaggatcac aaccagaagt
      181 ggcatattcc taaagaacca aacatagaga gtccctgcgt ctcttagtag gttgatcttt
      241 gagtctctag caagtgctac aatgattcca acaattacgg caagagcaag agcgatgacg
      301 ctaagctgaa aggtagtgag tatgccctcc agaaataact tgtagtattt ggttagacta
      361 ctccagttga agggatagac tgctgacact gatgaatagg aaaaccagag aagaccggca
      421 accattattc ctagagtaat tttcgacatc gctcttgaca tgtcgttttc cttagaattc
      481 gggttcgtat tctacgaacc agtaattttc aagctctgca agtgttccgg tagtctttat
      541 ccattgaatg aaagtgttta gccactgaag tgtctcgaag tcacccttcg gtatagccac
      601 gccgaaatcc tctctagtaa ggagatcctc ggttacctcc agctggtcat acttcttggc
      661 catgaatctg gcgtatatag agtcgaatat cattgcatca gcccttccgg aagctacctg
      721 gaaggccgcc tcgtcgattg tctcaaacat gaagatctga gcttcggaga gaagtctgtt
      781 cgcagcctcc gcacctgtcg atccaagttg tacggcgatc ttgacatctg gatcgttcaa
      841 atcttcttca gtaggtggga gttcatactt gttcgtgttg tagagaatta tctgacccac
      901 agttagatac ggatcagtaa agttgactct cagtgccctt tccggagtaa tagtcatcgc
      961 agaccagatg atgtcgaact tgttcgaaac gagagcggga attattccgt cccagtttac
     1021 aacaacaaat tccagcttca catctagtat ctcggccatc ttcttcagaa tatcgacttc
     1081 cagccctatg cggtcgccgt cgggattcgt tccgtaaagg ggcatatagc ctgcgtcctg
     1141 tccgactcgc agtacacctc tctgcttgac gtcatcgatg agacttccaa atgccagggt
     1201 catacagagt aaaaccaaaa ccagcagtaa cttcttcata gtacacctcc attaaaataa
     1261 aaagaccggg ctataagccc ggtctgttga attctgagag agcacgacta aagagtgccg
     1321 cctcccggaa ccgggcgccg cagtctgtga tgatgaagct tgtttgttct gatcgatgaa
     1381 ctgaaactaa ttctcacaga gatcaccttc gtcgtgtttt gataaacgat agtatcagca
     1441 ctggatatca aatgtcaagt aacgtttcgc gcaaaggtgt taagagaagg tttggggtcc
     1501 tatagaattc tcataatctc cttcgtgatc tgctgggcct tctccggatt gatcacaata
     1561 tcgacggatc tcgcatctat cttgtaaatc ggcgaaagat cgtaatctct tatccagctg
     1621 gagtaaagtc tatcgagttg ttcccagtag gcaataggga ccgcagtttc catttgtctt
     1681 cccctgcgtc ttattctcgc cagaatggtg tcaacatcgg catctatata gaccaggaga
     1741 tcaggcttct tgagatactt gatcatcgag tcgaacattg tcacatacgc cttgaattcc
     1801 ctgtcggaca tctttccggt ctcgaaaagg tttcgagcga aaatctcttt gtcttcgtat
     1861 attgaacgat caaataccgt attttcattc acctcggcac tctttaggga gcagtatctg
     1921 tggtatagaa agtaagtctg gagatgatac gaccatctct tctgatcgta atagaaatcc
     1981 tcaaggaagg gattgtcgga gaccgattcg tagtgaatat tgaaaccaag gccttcagca
     2041 atggcgcttg ctatagtaga cttgcctgca ccaacatttc cggcaagcac gattattctt
     2101 cccactttca tcccccctac cagatctaat aaacaattct agcatttcag cttctaagct
     2161 aactctaagg ttaatcaagt caaatagttt atcgcctggt tgaggatgtg aagactgtga
     2221 aaagtcgcca tagagtgcca cgaattgcta cgcagttact gtttctcctt cttgttctct
     2281 acataagtgt tggtcactat ctttctgaga agggcatagc ggagctggct ggcacgacaa
     2341 gtctccatgc tgtctgtccc ttcggcggtg tcgttacgct atacaatctt gtctctgatg
     2401 gcacctacgt ccagaagata catccttcca acctttttgt actgggtggc ttgctcattt
     2461 cactagtcat tgccggagct ttcttctgtg gatggatttg tccgctagga agtgttcagg
     2521 agtggattgg gaaagctggt agaaaactcc tcagatcaca ttacaataag gtcccagcag
     2581 gtctcaacaa aatcctaagt cttggaaagt atgccatgtt gatcttcgta attgttcaaa
     2641 cagctagaat cggcacgctt tcattcagta gtttcgaccc ctattataat cttttcaata
     2701 tttggacgga tgaaattgca ttgtccggtt atattgcagt atttcttaca cttggggctt
     2761 ccctgttcat cgaaagacct ttctgtcgat atgcctgtcc actgggtgcg atcaacggtc
     2821 tattcaaccg cttcagtatt tttcgtatta gaaggcaatc ttcaacatgt atcgattgtg
     2881 ggctatgcga taaggtgtgt ccggcaggga ttaatgtctc gaaggtttcc agtgtgaaca
     2941 gcccggcctg caacagatgc atgaagtgtg tcgatatttg tccagctaac tcaaattcaa
     3001 cacttacgat agaggtcggt ctttctaaga agtcatcaaa agccagcaag acagtatctg
     3061 tgaagactta cataatactg gctctctcag catttttggt gccggtaggc ctatctgtct
     3121 tgacaggtaa atttgagacg gaaaagacga gagtatacat agtccccgat gatattaagg
     3181 gttcaacatc ttttgaggag attgtcgaga actatccgat ctcgaaggaa cagcttttta
     3241 atggccttgg aatacctgag atggtttctg ttgagctcaa agtgaaggac cttacaggaa
     3301 ttcttggttt tatcggtgaa gaagaagtga tatcgaggga gcatctttca gcactgatcg
     3361 gaaaattagg tgaaccaatt tcatctttgg ccgagcatag caaagtggat tttgaaaata
     3421 ttatcgaatt tgtccagtca agtggtttgg acgagtcgac ggtcgtttca gaagttatgg
     3481 ctagtggccc tccagggctg ttcacatatc tcttcagcgg aagatggcct tttgaaacat
     3541 ctggagatgg agcagtcatt gaagagaaag agactggcag tgaaaatcat gggcaatccg
     3601 cttcagttga tatcaagggt tctacaagct tgttggagat cgaaaggatg gtcccggatt
     3661 ttgaagcatt ccttgaatat tttggcctgc cgatcgatga acccaagagc tcgttgttga
     3721 aggatctgaa gacaaagtac cagatcgaga tttcggatat aaaggattat gtttccaaca
     3781 atcagtagat ctgatatgaa tagcaatcgg ttctacctgc tcctgttgta taatcggaga
     3841 ggactccggt tgagttcata cacttagagg agggatagac atgaagaaat tgctgttgat
     3901 tcttgtcgtt tttggtattg cagttgccgt attttcagag ccccttttag tttggccaga
     3961 taagacacat gggaagccac ttgtggcggg attatattcc cctgtttatg gagaagccaa
     4021 actggacgtt tttggaaaca tcaccggctg gacgggtcca aatctcggtc tgggatggac
     4081 ctggaagaca tatttcagcc ctctcgagct tcagaaggtc aatttctatt acgagtttgg
     4141 tactaatgtt gtgattttcc cctatattgg ggtcggattt gattatgctc tcgttcttca
     4201 gaacaatcag acactgctag ttggagcagg agcttcggca tctcctttca ctgttctagg
     4261 tttcttcttt gaggggccgg cagctattct atcatctgtt ttgagttccg tgatactgag
     4321 cgtcgctgta gttttttgat tagaggctat gtataattga ccccgaaagg ggtcattgtt
     4381 ttgagtgaac tggagaacag tgggagagaa agagatcagg cggagaagaa agtggacagt
     4441 gaagtcaaat ctgtccttac caaatacaca tttgttgcag tcttagtact tgcagtggtt
     4501 ctgatgcttc tctcttcaag aggcagggta gaggagttcg atccggagag tgtgtttcca
     4561 actattgagg agacgtctct tctcttcata aatgagaacc ttcttccgga aggcgaggag
     4621 attgaacttc cggagatagt ctccatcgac tacagtgaaa tcgagtgggg attatactcg
     4681 tatgaggcta ttgtgaaata ttacttatct ggagctgtca gaagcctcca cactgtctgg
     4741 gagtacagag ttcgagatga taccctggat ctcttaggat actcttctga tggtgaaaac
     4801 tggttagaac cttgatggtt atcacgcaca ctgtaggcca gaaattgcat tctgagattg
     4861 caaatacgtt gacttgatgc gggtattaag tgtaaaatcg ttaagtaaga acaagctcaa
     4921 tatcactcaa aagggggtaa tcgaatgaaa aaagtcctga ttcttgtggc agtccttctt
     4981 gttgcatcgg ttttcgttgc agcaccgaag aacttcattc tcggccaggg tctctacgga
     5041 gatgtagtgt atgatctagc tggaaacata acgggttatg aaggaatgga cttctatatc
     5101 ttccaggcta gcacttacta cgaaaaggca ctgacaccag gtgaactaaa tgcctataca
     5161 tttgatggca aattcctgtt ctttattccg gtttttggta gaggatggga attcgtgctg
     5221 agaccagtgg acaatattat tttgaccgct gggctaagcc ttctttataa cttcctcccc
     5281 gggccgtatc ttggtctgac aatattactc tgagcaatct gtttatggga gaagacggtg
     5341 tttctgcacc gtcttcttct tttgataagg cgcaaaaaag aagcagtttt tttgaaccgg
     5401 taagcaatgt ggggtaacag tgacagacaa atttctacta aggacttctt cttctcggat
     5461 ttctggcctt ctttaacttg acaattaatt catctagaat tgtacagttt gcattgttat
     5521 tcagaaactt cttgtaggtt accctcagag aagagaattc tactcgtaat ggttggtcaa
     5581 ggaggttcga ttttgagaga acttaaaaag gaagcaaagt atattgttga gagatcaatt
     5641 gaaacggttc agcctgaaag ctccgttaag aatgctcttg aaaagcttag gctgaaaggt
     5701 cctatattcc ttttggcggt cggcaaggca gcctggagaa tgaccaaagc tgcgatggac
     5761 aagcttggtg aagaagtaag cgcgggaatc gtaataacga aatatggcta cagtcaggga
     5821 gatatcgaac gcgtagagat tttcgaagcc gggcatccga tacttgacag aaagaccgtt
     5881 gaagctacag attatgctct caagaaaatt gaggaattga agccggaaaa gattctcttt
     5941 cttatttcgg gaggcggatc ggctctcttc gaaaaaccat tggagggggt gatgcttgag
     6001 gatcttgttg atgtcaacaa tcgtctattc cgttcaggtg caagcatcgt cgaaatcaac
     6061 acggtgagaa aacacctttc ttcagtaaaa ggagggcgtt ttgcaaagct ggtctctccc
     6121 tctcaggtct atacgcttgt tctttccgat gttttaggag accgtcttga ttcgatcgct
     6181 tcaggaccgg cttaccccga tagcagtaca agtgaccagg cgcttgcagt ccttgagaag
     6241 tacgctattg atgttgattc cagaatcatt gaggctctga tggtagagac tcctgaccgc
     6301 ctgtttaacg ttcaaactga gattatcgga agtgttgcga gagtctgtga atctgcagca
     6361 acatttgcca gagagattgg atataacgcc actatcctca caacttcgtt agattgcgaa
     6421 gcaagagatg ccggtgtttt tttggcatct atcgctagag aagaagcaca caacagacca
     6481 gtggaaaagc cggccgctct gatcattggc ggtgaaacag ttgtgcatgt tagaggcaga
     6541 ggtctgggag gaagatgtca ggagcttgct cttgcattct ctttagggat tggggaaatg
     6601 ggtggtgtta cgctggcctg tgtcggcacc gatggtaccg atgggcctac cgatgcagct
     6661 ggcgccattg tcgatggaag aacagttcga aaaatgttgg atgctggcat agatccacag
     6721 ttatttctgc tcgacaacga ttcctaccac gctctggaaa agtcaggcga cttattcatt
     6781 acggggccga ctggtacaaa tgtaaacgat ctgatagtgc tgctttgcga gtgaatatag
     6841 aaatacgatc gacagtcatt aaggaaattc gattccttca aaaccagctt tacgacggaa
     6901 gaaggccgct attttattgt ccatccgacc attcctttga cgtaatatct ttgaagcaat
     6961 acgtagataa gaattggaac gatcatcacc atcaaagatg cagcggagag tagtccccag
     7021 tcgacatggt aaacccctcg cattaatgga atcctttgag ttgccaggag tttgtccgga
     7081 gaatagatga gaataagagc aaggaaaaag tcgctccata cccatgtaaa ctgcaaagca
     7141 gaggccgaag caattgccgg gaaagcattg ggaagtacaa tcctgaagaa gatacccaag
     7201 tctccagctc catcgattct ggctgcctcc tccatatcgg tcggaagagt cttgaagtaa
     7261 ttacgcatga agaaagttat ccatggtatt ccccacgcag tgtggagaag aataagaccg
     7321 aagtaagtgt ctaccaaccc tagagtgctc attattttaa agataggaat agcaatcatc
     7381 tgctgaggga gcgcaagagt gagcacaacg gttagaataa acgtcttcct gaacgggaat
     7441 ccatatctgg aaattccata gcctgccaat gttgctagca ttaaagggat tatcgtagag
     7501 ggaagagcaa ttaacagaga gtttatcatc cccctataaa gtggtgctgt acgatgatta
     7561 agtgccccgg tgaagtttga tagagttgca gtgaacggct tgagactcca ccatccgtcc
     7621 agtacttcac taaagggcct aattgctgtc ataactacgc ccatcaacgg aaggatccat
     7681 atgagggcga taatccaggc aatgactgtt tttagaacaa tctctctcct tgactttctc
     7741 attctgcctc atctcccgcc gatttcatca tgggaatcgc agccagtaga gttgaggcgg
     7801 taattaaaac cgcaactact gcagattttc caaagtccca ctctcgaaag ccataaagat
     7861 acatttgaag cgcaagaacg tttgatgaac cacctggccc tcccatagtt gcaatataaa
     7921 caatatcgaa gaccttcagt tcccacagca gagtcattgt aagtacgaca acggtgattg
     7981 gtttcaacaa tggaatcgtt atcttaagta gcattttgaa ctttgatgcg ccgtctatct
     8041 gggctgcttc atagtatgtg ttgggaatag tctcaagccc ggcagaatag agaatcattg
     8101 agaagcctgt ccacaaccag acagagccga agatcagtgc aaagagagcc gtctctggat
     8161 atgctgtcca agtcttacct tccagtccaa gtgtctgcag aactagattc acaacgccaa
     8221 cattttcctc gaacatgaat cgaatcatta ttccgcccac tatcattggc ataaccatgc
     8281 caaggaatat tatcgacttt actattcccc ctccctttac gtctcgcaga aggatagcca
     8341 gaaataaacc cacaagggta gttaatggca gatggatcgc gatccacatt aagttatgaa
     8401 taagtgaacc aaaaggaaaa cctctctcta gtccccgggg atctaggata tccctgctgg
     8461 aaaacacttc tctgtagttt gagagtgaaa ggttcccttc gctgtcaaca aagctcagga
     8521 aaatcgtgtt gaacgtagga tataaaacga aaatcactag aagcaacaac gccggcaaaa
     8581 tgaagtagct gaagtgcctg aattttgcct ttctcaaagc caactagatc acccccgaag
     8641 aaagaagtgg gacccagatg ggccccactt agagaaatcg attacttcgg cattttttca
     8701 tcaaggtcca tcagcgcatc gtcaagcctt tccggtctta cccagattag tttaagctgg
     8761 tcccagaatg ctgtctgcca gagaccgccg atggaatcat cgagatcatt taatgtctca
     8821 acaccctctg tcaacttcgc aatctccttg tcgactgcag gataattaga catgtcgact
     8881 ccgacggttg caatatggcc accctgcgca acctgaatgc tctgaccttc cacactcgaa
     8941 aggaatttca caagttccag agcttctgcc ttgttggttg cgtattcggg cacaaatgca
     9001 tagtcaatac tgaaaaccat cccgcgattt cccggaagac tgaagacagc cagatcattc
     9061 gggtcgtcta ccattcccgt tatccactgt cccatgaagt aaagaccgta atctccgttc
     9121 caccactgct cgagtatagt tgtccactct gtgggttcac tgaagtatcc cttttcgaga
     9181 agatagacta gcttttccat ggcgttcctg accgcgtaac tcctccaggt cgtgtttcct
     9241 tcgattaggt ccttctggag ttcggggcca ccaaaggtaa ttaggaagtg ttcggtgaca
     9301 tctgaaagag gccaccctac tccattgccg cttgcaatag cgtccttcac tcctggtata
     9361 gtctcgattt tttcaaggag agcaacaaat tcatcccatg tagtaggtgc cttcaaacca
     9421 tggctttcaa agaaggattt cctgtaccag aaccccggtt tgacttttgc ggtgtaagaa
     9481 atcccgaaga ctctcccgtc tgaagtcttg acattgtcga gtgcacccgg aatatacgca
     9541 tcggtatcaa tcacatcaga taaatcaacc acgtgttgtg tgttgcttgt aatgaagttc
     9601 gaccacatga agatcacgtc tgctggtgcc ttctttgcag aaaactgtgc cggcagaaca
     9661 tttgccaggt cttctgccct gtatgtctgg taggtgtaat caatgcctgt ctttgccttg
     9721 aattcttcga ggactggaag gaaagcgtcc atctcaacgc cagaccaggg accaataacc
     9781 gaaacagtgc tagagatcaa tgcggtggag agaacaacta ggactgttac caaaagcttc
     9841 ttcatcaaaa ataccccctt ttcaatgtag attcgctttt cagtaaacat ctcccttaga
     9901 ccgcttaagt ggtctgaaaa tccacacatt catccgaaaa tacacacatt atatggtttg
     9961 actcttgtaa cctgaagtta gttcaacaga aatcaatgac ttagtaagga atcagactta
    10021 aaagaagaaa catcacaatt tttcaataaa acaattctgc tttttgactc atacgtagaa
    10081 gctgattgcc atcttattca aaatacaatt ccttttgaaa atggaaatga tatatggtgg
    10141 ctgtgatgaa cttttattat tattattttt ttatgaaaac tatctccatc ttattgagaa
    10201 caagtcttgc tggcgcttct tgtcgagtgt cgctcagcga agagagacag tgacagttgt
    10261 cattagttct tagttttatc tcaaggacac cttatgtaag ctatcattca tttttacttg
    10321 cttagtctct cttcaatact gccgtctgga aggacaaaat ccgtcgagac cgatagtaaa
    10381 tcatctaggc gaaatctagc acattctgtc caatcctttt cacagtacat ctcgatccat
    10441 tccctgttga tctttccatt ttcgtaagcc cttacaactg gacactcatg ataattgatg
    10501 caatacccat cctccaccag atcaccacca ttctgtagtc gtcaattatg ttatcacaac
    10561 gcgagaaaat tgcaataccc accatctgag tttggtagtt tgtaatattc tttgtatccg
    10621 ttatacaatg tttattatga ggatgaaaaa acgggggtga tctaatgcac agaaggctgt
    10681 cgtctttatt actaatctta tgtttggttt tggtcattgc actttcggga tgtgtttttc
    10741 gacggtattc agttgtagtg agctgtacac cgttgattga aaagatcgaa gtagggaaca
    10801 gagagaggga gctgccgtac agcttttctg ccagaaaggg acagaacata gttgtaaagg
    10861 caccgctcgt tcccggatat gactttgaag gttggttgat aaacaatgaa ctgagacaaa
    10921 cggcggagat cgcagttgaa atagaagaaa acacaacaat taaagcgata tacagcccaa
    10981 gacttgttca cgagattacg tatggaggtt caggctatga aaatgggacc ggcctggttg
    11041 tcgaaaactc aaatgagatt ttaataggaa tcacttcaag ttccaccgac agctctctta
    11101 ttctcgaaaa caaaggcaag agtgactttt ggattccttt tcttcaatac gaatcaaata
    11161 attatgttgt ggaaccacag ttgtgttatg gaggaagcga tgaggagtca cttatggata
    11221 cagtcagaat tgctggcggt tacgtatcgg ccggctggtc ggactcaagt gatatagcat
    11281 attccggaag cggttatcca tatcatgggg gtactgacgg atacgttatt atgatgcaca
    11341 acgatggctc actcagatgg cagacttata taggtggaag cggagacgat gagattcgtt
    11401 cggtgaatac cactataggc gagaagctta taatcacagg gattacaggt tcttcagact
    11461 ctaatttcgc aaacagcggc tacgacgggt caaaggatgg ttgggtagcg ttgatgagct
    11521 ccagcggtac tctcatcaca gacctcatta aggtctttgg tggcagcgcg gaagacggtc
    11581 ttctctacgc tatgcagaca aaggaaggtg gatacatcgt ctgcggatac tcgagttctt
    11641 ccgatggaga tctaaattca ctcagcagat cgacgggcga ggacttgtgg gttctaaaac
    11701 tcgattctca gctggaaatt gaatggcaag atctcttcgg cgggcagaac gatgaacgtg
    11761 ccaatgtgat aaaagaggtc agcgatggat atatagcagt tggtttcact gaatctgccg
    11821 gaggcgacat caatgagaac aaaggtggga aagatctctg ggtactgaag ttctcttctg
    11881 acggtgatct agagtggagc aagacatatg gcggaagtga ggacgatgaa gggtttgacg
    11941 tcgttcaaac tgcagacggc ggatttgcaa tatgtggtta cactgactct tcagatggcg
    12001 atatccttca cgaaaataga ggtatgagcg acgtttgggt tataaagctc gggtatgacg
    12061 gttacttaga atgggaagct acttttggcg ggtccgaaaa tgattttgca agaagaataa
    12121 atgaaaccga aaaaagagaa ttgatagtaa ttggcacaac ttcctcatca gatcgcgata
    12181 taaaacgccc gatacaaggt ccttccgatg cgtggttact gaggcttgga agatgagttc
    12241 cgtttgccgg tgtgctgttg aaagcagata atgttcttga aagcgagata atttcccttc
    12301 ttgaagaagg ggaactatgt cctgtctgca gatctatgga gaaatcagtt gatgggtgga
    12361 tagtcggagc cttttccaac cttcttcaca acgaaggtgc tcgaatggaa ttgagaagaa
    12421 atgggctttg cacagttcac ttaaagagaa ttgtggaagt agcaaaagag agatccgaag
    12481 taggatccct tgcagtctcg ataatgttca aagagattct tgctgcccaa gtttcgtggc
    12541 tcgatgaaaa agatgtcgga ttcttgtcta gaaaaaagcc gaaatctggg agctgcgttc
    12601 tgtgttccgt ggccagtaat agtgagagaa tctatctttc tgccttcgtg aagttccttc
    12661 aaagagatga agtacagagg tcatatgaga actccagatc cgttttttgt gtgaatcatt
    12721 caagatacat catcaagaat atgagaaatt catgcgggca gtggctagct ggaacccaaa
    12781 gaaataaata tgaggacctc acagcagaga tggaaaacta catttctaaa catgattaca
    12841 gaaacacaac tcccgttggg caggaaagac atgcatggtt aaacgcatct aagttgattg
    12901 gggagaggga tgcaaaaaac agttagtgaa aagaacaaaa gtcaaacctc actggttctc
    12961 taccgattaa atgatgcagt gaatttattc tggcctgtct tgatcgttta gaattgagag
    13021 cttccggtgt gcacctttat tttcatgttc tgtatcagtg attattcctc agtaaactga
    13081 aaaggcccct cacggggcca gttgcaggaa agcgcttccg ttggaagcct ccggggtgac
    13141 tttatgcaac tcgatgcata gtttttgctt ttgcctttca aaggttctta tcgaatcctt
    13201 ctagtacagt tctttactct ggaagtctct cttcctgttt aatcttgtgg cgacaggaat
    13261 tactgagagc cccaaggcga actcttaatc acaagccgag cgacgcttta aaaagtcaag
    13321 agtagaactc tttgctgacg tttctatatt tactatcata gcaaaaatat tctttgtttt
    13381 cagggataat tatatctacc ttttcaaata tgatctatat attcatacta taaccaaaaa
    13441 aattgatcta gtcaaggcac aaattcaata gttatatgta agtattaaat atttcatgat
    13501 gcatattctc aagaattgga tatatatctt atccgtgaag gtcggcgaat cacaactttt
    13561 ccctagtaac atttgttact gctgtaaata aagattcggg ataggctctt tatgaagaca
    13621 atgaggaggt gaaatgatga gtgagctttt caacaaagaa gagtttctta aaggattgat
    13681 cagaaggctt catgaagagc ccgataagct tgagaacatt agagaagatt tcatgagagt
    13741 agtgagagag ctgacacctg tagaaatagc acaggtcgag cagaaacttg tggaggaagg
    13801 tatgcctccc gagagcatcc agctcatgtg caacatccat cttgatgtat tcaaggaggc
    13861 tctggacgaa gacagtattg atgttggctc ctggcatcct cttcacatat tgattgaaga
    13921 acaccgcgac attcttaaca ggacgaagga attgagagat ttgaccggga agattatttc
    13981 tggaaattac gatggcgaag acataagaag gcttcaatct tcaatacaat atctcgatag
    14041 tttagaggta tatttttcca aagaggagaa tgtgcttttt ccctatcttg aaaggcacgg
    14101 tctggttcaa cctcctgcga tcatgtggaa ggaacatgac gaagttagaa acctacgaaa
    14161 gaagatgaaa atcgaatatg agaacctaaa gaatggagac ccaaataagg taaacgagct
    14221 cgcaataaca attagcgaac tcttcacaaa tcacatctac aaggaacaca agattctctt
    14281 tccttcggcg cttaagctgc tttcaaataa tgagtgggaa tctattagag tggagttcga
    14341 tgaaataggg tacttttcat ttgagccgat gcccttcgtt tcctctgaag aagtatcaaa
    14401 aaacaacggt gttgaagggt tagtaaatct tggaagtggt tttctgaagg tagatcagtt
    14461 actcttgatg ttgaacagcc ttccttttga tattactttc gttgatgaaa acgacaccgt
    14521 ccggtatttc agtgaaggga aggagagaat cttcgttaga actagagcaa taatcggtag
    14581 aaaggttcag aactgtcatc cgcagaaaag cgctcacgtt gtgaccagga tccttgaaga
    14641 tttcaagaat ggggtgcgtg atgttgcaga attctggctt aatcttggac caaagattgt
    14701 tcacataaga tatttcgctc tgcgggacag atcaggaaaa tatgcgggaa cactggaagt
    14761 gactcaggag atctcttcta ttaaggcact tgaaggagag aagaggattt atgatcctct
    14821 tgattagcta aaaatcgact aaatcgaaaa ccccatgcag atattgtaga atagaatgta
    14881 cttaccatta tcttcatctt gctttcggag gtactgttat gattcggaga gaaatgggaa
    14941 agactggaga aaaggtatct gccttgggtt ttggttgtat gagacttccc acgtgtgacg
    15001 atcaatcaat agacgtggat gaagccacaa gaatgttgag atttgctata gataacggtg
    15061 ttgactatgt cgatactgcg tggggatatc acaacggaca aagtgaaccc tttgtaggca
    15121 gtgttcttca agatggctac agagataagg taaatcttgc tacaaagttg cccagctggc
    15181 ttatcaagtg tagagaagat atggactact atttgaatga gcaactcagg agattgagaa
    15241 cagatgtaat tgatttctat ctaatccacg cgttgaacag aagatactgg aagaatctca
    15301 aggaacacga catattcgag ttcatggatt ctgctcttgc cagtggaagg atcagacaca
    15361 tagggttttc ctttcatgat actcttgatg tctttaagga aatcatcgat tcttacggct
    15421 ggcagttctg ccagattcag tacaatttcg tagatacgga gcaccaggcc ggtctagaag
    15481 gtttgaaata tgctgcaggt aagggaatcg gagtagctgt tatggaacct cttcggggtg
    15541 gaaagcttgc cacgaatgta agagatgaca ttctttcgat ttggacgtcc tcgggaatcg
    15601 atcggacgcc ggccgcctgg gcgttgagat gggtctggaa cgacccggat gtaggtgttg
    15661 ttctgagcgg gatgtcgact atggaccagg taagggaaaa catcaagaca gcaagagaag
    15721 caagtcctaa tttcatgagc tcttctgaac tggctattgt tgatagagtc agggaggagt
    15781 acaaaaagag gataaaagtg agctgtacgg gttgcagcta ctgcatgcct tgtcccagtg
    15841 gagtagcgat cccaacttgt tttgatttct tcaatgatgc gtacatgttt gacagcatag
    15901 aggatcataa aaaagcttac ttgagatctg tcaagaaaga gaacagagct tcactttgtg
    15961 tcgagtgtgg acggtgtgaa gagctttgcc ctcagaacat tcctataatt gaacaactta
    16021 aggaagtctc ttcactgttt gaataactgc aaaaggatca gtgttgtgtt tgtgtctgta
    16081 ctctgccttt ttcagctcga gtgctatata ataatcccgt gaacgaaatg aaaaaacctg
    16141 tcggggaggt tgtactaaat gactggaatt gtgctggccg ctggccaggg aaagagaatg
    16201 aaatcaagat tcccgaaagt ggttcacaag attcttgatc ggccaatggt aaactgggtc
    16261 atttcagcgc tcagacaggc cggttcagac agaatcgttg tggtcaccgg tttcgaggct
    16321 gctatggttg aagccgttct tgacaaggat atcatttctg ttaggcagac ccctcaactc
    16381 ggcacggggc acgcagtaat gacggcgctc gattatctcg acgatgaaga catcgttgtc
    16441 attgctggag atgagccgct tgtcttacca tcgacattga aaaagcttgt ttcaaggagg
    16501 attgaagggg attttgatgt tgtcttcctt actatgaagc ctgccgatcc gacgggatat
    16561 ggaagagtcg ttaaagacgg caaaaagata aaaatagtgg aacacaggga tgccgattcg
    16621 gaaacaagaa tgattgatga agtaaattcc gctatatatg cttttaaggg gagttttctg
    16681 agttcagcaa tcaagagttt gtccaacaat aacgatcaga atgagtacta tctcacggat
    16741 acgatttcgc ttgccgataa agcggatact atagttgttg aaaatgctca ggaagttctt
    16801 ggtgttaatg acagaattca gctggccgag ataaataaga ttgctcagaa aagaatcaat
    16861 gtagaacata tgagaaacgg ggtcacgata gtcgatcctg acacctgtta catcggtcca
    16921 gaagttgaga tcggtgctga cactgttatt gaacccatgg tttttctttc aggaaagacg
    16981 cgaatcggaa actgctgttc tattgggccg ttaacaagaa ttgatagctc agttatcgga
    17041 gacgatgttg agatattgcg ttctgaagta tcgaatgcag aggttcattc tggcgcaaga
    17101 gtcgggcctt tttccaggtt aagacccggg gctgtagtaa tgaatgaagc acatgtcggc
    17161 aactttgtgg agctgaagaa gacgattctt ggcaggggat caaaagcaca gcatctgaca
    17221 tatcttggtg atactgatgt gggtgagggt gtaaatatcg gtgccggaac gataacgtgt
    17281 aattatgatg ggaagaggaa acacaggaca gagatcggag atggggcatt cattggcagc
    17341 aatacttcgc ttgtcgcccc ggtaagaatt ggaaaaaact ctgtgactgc tgccggttca
    17401 gcgataaccg aggatgttcc gcccgactca ctcgcattcg gtagagcgag gcaagtcgtc
    17461 aaagagggga agtactcaaa aaatagaggg caggaggaga acagctgatg ccgtatcaga
    17521 ctaacgagat gaaagtattc gctggctctg cgagtctacc gctggcagag aaaatctcaa
    17581 attacttggg aatcagactt ggagactgca ggacaaagag atttgcggac ggagaaatca
    17641 atctgaaaat cgatgagact gtaaggggtc atgatgtata tataattcaa tccacatgca
    17701 gtcctgcgaa cgagaatctg atggagctcc taataatgat agatgcattt aggagagctt
    17761 cggctcattc tatttcggcc gtaatcccct actatggata tgcgaggcaa gatagaaagg
    17821 ctagaggtcg ggatcccata actgcaaaac ttgtggcaaa tcttctcacg acttcaggag
    17881 caagccgttt aataactatt gatttgcatg cagaacagat acaggggttc ttcgatattc
    17941 ccgtagacaa cctctggtct tttcctgcat ttctgaagtt cttcagtcag agtgcctttg
    18001 atcgaaatga gatggcggtg atttcaccgg acattggtgg agtaaaacgc gcaagcaaat
    18061 ttgctgcaaa gctgggtgta ccgctggcaa ttttggacaa gaggagacca gaagacaacg
    18121 ttgcggaaat ggttcacata atcggcgatg ttgagggaaa gacagcaata atctttgatg
    18181 atataattga caccggtcgt tcactagtgg aagcggcgaa gatgctaaaa tccaacggcg
    18241 ctgacaagat atttgcgtgt gcgactcatg gagttttttc cggtgatgct aaaaagatca
    18301 ttgccgagtc aacgattgaa aaggtcttca taacagacac gatttaccat gatgaccttc
    18361 ccgataacat aatgatgtta tccatcgcgc cgcttctggc ggaggcattg actcgtgtaa
    18421 gaaaaaatct ttcggtgagt attttgttca gatagaggag ggaaacaaat gcacgaaata
    18481 aatctcgttg ctgaacttcg caacggtgaa atgaaagcaa agagcctgct gaatcaaggg
    18541 aagattcccg cggtagttta tggtcctgag ctggagacag ttcctgttag tctggataag
    18601 gtagagatct tgagaatcat gaacaggatt gccgagacta cctcggtggt catttctatc
    18661 gatggaaagg aatatcatac ctttctcaag ggtgttcaga gagataaagt aaccgactcg
    18721 gtcattcatc tagacttcta cgttccttcc agtggtcaca agatggaact ccgaattccg
    18781 ataagattga agggtacccc gataggcgtt gaaaaaggtg gaatagttga tcatatcatc
    18841 aatgaactgc ccgtttcggt tctcccaaag gatattgtgg aagagatcat tgttgatgta
    18901 agttcctttg atgttggcca tgttctgcga gttggagatc tcgagattcc cgagggaata
    18961 aaacccttgc ttgacgatga agacgctatc atgctcatag cccttccgag agcagtaaag
    19021 gaagtcgaag aggaagaagc cgaagagatt gaagagggtg ttgagcccga ggtaataaac
    19081 aagggtaaga aggaagagga agaagaggag taatagtgtt tctcttcgtt ggactgggaa
    19141 atcctggccc acgttatgcg ctaacaagac ataacgtggg ctttctcttc atagatcaga
    19201 tggctaaact tggcctaagg aagacagtgc aaaagagact gtatgaagca catcttcttg
    19261 agggagaaga gaaagtctat attgcaaagc cacttacttt catgaacttg agcggagtgg
    19321 ctgtaagact tctcatgaaa gacctcaatt tcaaaaggga agatacgctg gttctagtgt
    19381 atgacgatgt atggatacct cttggtagga ttaggataag agaaagcggt tcagatggcg
    19441 ggcacaatgg tctgaaatca gtgatttcgg agataggaac tcagagcttc ccccgaatca
    19501 gaatcggtgt tggacccctt ccagagaacg gagatatggt agattatgtt ctcggtaggt
    19561 tcagcaatga tgaataccag aaagtcagca aggtaatcga cttagctgtc tccgctgcaa
    19621 aggagcttat ctccggtgat attcgaaagg tcatgacagc ttacaatgga actgaagtag
    19681 attgattctc atctgaagcc tcccaaaact ctttaagacg tttttctaat ctaggtttaa
    19741 gcgataccct tccaaagtct ttaattccaa aggtatcatg acactgattt catttctaga
    19801 tctgggagaa gtacgttgaa gtttatcctt ccagatttgt gagatgtaag agctttgcag
    19861 atgaataacc gctttataaa agcagcccgc aagcttccag ttgaatccat acggttttga
    19921 aggaagtaaa ttggaagcga ctaagtagag tgacaactaa gtgttggctg attttccagg
    19981 cattgcttgt gttaaagaga ttcctgattc agaaacatga gtcaaatgag acgcccgaaa
    20041 ttcccagaat cgtcaagatg agtctacacc acttcctttg tgcgatatga gattatcgag
    20101 aacccaccgc tatatttgca ggagatgcga caactattaa atgattacct gttcagctga
    20161 gacttatggg ttgtcaccat tgaactgtgg cagtcgggat gcttgtacca gagttaagag
    20221 cagaacgaag aaagagagat acgcacagct aaaggcagaa agattagata ttctgtttgc
    20281 tcttcttgag gctgtacatt tcatctcgca ggattgcagc gtcttcaaac ctcaactctc
    20341 cggcagccct tatcatctct tcgttgagta aggcgatgta gtcttcatag tctagagact
    20401 ccttcaacgc catcactcct tcaagataag tggctctcgc cttctcttcc ctctccttgt
    20461 caagattctc gccagcgaac tcctcgaaga tgtttctaaa cagaggtttt acaatgctct
    20521 cgggtgttat gttgtgttct ttgttatata tcagttgttt cagccttctc ctgtttgtct
    20581 catcaattgc tcttttcatt gagccggtca tggtgtcggc ataaaggata acctgaccgt
    20641 tgacatttct cgcggctcta ccgattgtct ggatcagtgt tgtctcagaa cgaaggaacc
    20701 cctccttgtc ggcatccatt atcgctacaa gtgaaacttc tggtaggtca agaccttccc
    20761 tgagaaggtt tacgccgacg acaacttcca cggttccatc cctgagtttt ctcagtactt
    20821 ccactctctc gattgcgtcc agttcagagt gtagatactc agatctgatc cccacttcgt
    20881 tcaagtagtc ggaaagcatt tctgctgcct tctttgtaag aacagttacc aaagctcttt
    20941 catttctctt ctttacttct cgcatccttt caatgaagtc gtctacctgg ctttcggttg
    21001 gatgaacaat aacctcggga tctacaagac cggtaggcct aataacctga tctataattc
    21061 tagtcgaatg ctcagcttcg aaatttcccg gggttgcaga gacgaagaca atttggccga
    21121 ccgtgcttag aaattcttcg aatttcagcg ggcgattgtc aaatgccgca ggcagtctga
    21181 atccgtaatc cacaaggttt ttcttcctgg aataatctcc tctgtacatc gctcccagtt
    21241 gtggaacccc aatgtgagac tcatcgataa acgtaataaa cttttctctg tcaaagtaat
    21301 caagcaatgt atatggtttc tctccaggca ttcgcccatc gaagaatcta gagtaattct
    21361 ctattcctga gcagtaaccg acactagaaa gcatttccat gtcgtaagta gttcgctgtc
    21421 taagtctttg cgcctccagg tactttcctt cacgttctag ctgctttacc ctttcttcaa
    21481 gctcattctc aatgttccgc atagcaccaa gaatcttatc ttgagttgtg acaaattcct
    21541 ttgccgggta aacagttacc atgtccagtt cttcaatcgt ctttctgtta ataggatcga
    21601 aggtcacaat ccgttctatt tcgtcatcaa agaagtacag cctaatccca aagtcttcgt
    21661 atgggggaaa gatttctaaa atctctccct tcacatgaaa aactccacct gccgatacat
    21721 cttcggaacg attatactga atgttagcca gcttgagcgc aagctccttg cgattgatac
    21781 tcttcccgcg ttcgaggaaa atattcatcg ttgcaaaatc ctttggatca ccgctggcat
    21841 atatcgcgga gacgctcgca agtacaaccg tatcgtttct tgtcaaaacg gacttcaaag
    21901 ccgaaattct cattctctcc agtgtctcat tgatctctgc ctctttttct atgaataggt
    21961 ccttcgaagg tatataagac tcaggcatgt aataatcgta ataacttatg aaaagctcga
    22021 ctttgttctt ggggaagaag gtcttgaatt ctctgtacag ctgagccacg agggtcttat
    22081 tgggtgaaat gactattgca ggtctctgga gagtgcttat aacgttcgcc attgtaaaag
    22141 tcttcccaga accagtaaca cctagaagag tctgaaacct atccccactc ttgagtccgt
    22201 caataagctt ttctattgct tgcggctgat cgccttgcgg actgtacgag gaatccattt
    22261 cgaagagcaa aagatcaact cctttcaatc ttaccggcaa acaattttac accatcaaaa
    22321 cccattgctt caatgacgtg atgacttcct gcctgtcaac aaaaggtacc ccgttcaaaa
    22381 aactgctggc gaatcatccg gtagaaatgg ttttggatgt gtggacaaaa agaaaaatat
    22441 gagtaaatct ccggagggat atcttcgatc attcttttct cttttgctgt tacagttctt
    22501 tcagtttgat tagcaagggg caaatttgtg ttttcaagaa gcatgtaaat gctctactac
    22561 ttacggatct ccttcagtgc tttctttata agatcgtgag tatcgatttc cctttcagtc
    22621 ttcagaatct ctataactac ttttctcgac ttgttttcgt cgaaaccaag ggatgtaagt
    22681 gccgcaacag cttcttcacc gtgcgacctc tcaacactcg gaatggtttc ttcgtctgta
    22741 attccagaag cctctaccaa cgacgcgagc tcaacgatca ttctttccgc agttttcttt
    22801 ccaatgccgg gaagagaaga cagctctgcc gcattcttgg tggaaataat atgcttaatc
    22861 cttgaaggag tggccgaaga gagaatcttt atggcggact taggtcctat cttgcttgcc
    22921 ctcaacagta tattgaacaa ctctttctct tcttgatcgt agaagccgta aagttcaggc
    22981 ggcctatcct gtgagtaggc catgaacgta agaagataaa cgtccttttc gaccggaagc
    23041 ttgctgattg tgttcggagt gcagttaact tttaaaaaca gacttccgac ctgtatgaca
    23101 atttcggtct ctcttatttc tcttacgcga ccctcaatac cttccagcat gctcactcac
    23161 tccagttaag agatttctta tctcgctgac tagatataga gatccacaaa caagaagcga
    23221 atcggagatt tcaagcgcct tgttcaaggc aatctcatgt tcttccacga attgaacaga
    23281 tgaacaatac ttgcgccaga catcggcgac cttctcggga tgtatacttc ggtggtttgg
    23341 aaccctgcac acaacaacac tgtcggtaat ttccgaaagc actttgatat tggtttcgta
    23401 gtctttgtcg tctagacttc cgaagagaag cgtaagcctt tcacctggaa agtacttctc
    23461 gacagtcctc ctgagaactc tggccgcatc ggggttgtgc gcgccgtcaa gtataactgt
    23521 cttggaatcc tgagaaaaga cctcgaatct tcccggccat gagacagatt tcagcgccat
    23581 ccgcagtttc tcttcatcca aaccgcttcg aatctctccc attgcaatct ctgacgtctt
    23641 gattgccaaa gctgcgttta cagtctgatg ttcaccattt agattgatct gtaggttttc
    23701 aaagttatta cttccagtgt agtcaaacat attgcgattt agcgcatact ctcttccagt
    23761 cgcgtcgaaa tcccttttgt agaagaagta tggtgaattc atgcttcttg ccttttcaga
    23821 aacaactctt cttattgcag gtcttgttat acctgaaacg actggacatt cgagcttgat
    23881 aatgcctgat ttctcgatag cgattttttc ttcagtatcc ccaagaattc ccgtgtggtc
    23941 caaacctacg cttgtaacaa cagatacgag agaagaatca attacgttgc tagcatcaaa
    24001 tcttccacca aggccgactt cgagaacgac tacatcgcaa ctttcttcct tgaaatactg
    24061 aaacgcaatt gccgttacca cttcgaaaaa gctcggcgca tattcttcac cttttctgtc
    24121 catcttttct agaaccggtt ctatgttctt caggattcta cagacatcat cttccgatat
    24181 gtactcgcca tcgacttgaa tcctctccct gaaagttgta atgtgcggag aaatgttcag
    24241 ccccgtcttt agtccatgag aagacattat tgacctcgtg attgccgcaa cacttccctt
    24301 gccatttgta cctgtaatat gaatgatcgg atacgacttc tggggattgc caagccgttc
    24361 aagcagttct ctgatcctga acaggccata cttgatcttt ccatagggtc ttgagttgta
    24421 aagatactga attgcttccg agtatgtttt catttgagat cttctatgat tttctcaatt
    24481 ctgcgaatgt tatccttgca tgtcgcgagc ctttctctgt tttcagcaat tacctcttcg
    24541 ggtgcattgc gaatgaagtc accgttttcg agcttcgata aagttcgggt aagattgccc
    24601 ctttcctttt ctagctttcc cgttaacctc tcgatctcag catcggtgtc gatctctccc
    24661 aagactacat acaacgtgtt ctctgcatct ccccaggccg agacgcttcc ggaggtcttt
    24721 tctgcccttc tatctatttc gtttgaattg gaaaggtgtg aaacaagttc aacgacttcc
    24781 tgattcagga cttcaccgat gtagtagaca tcggttttcg aagagggggg tatgttcatc
    24841 tctgccttga tgtttcttac tcccctaacc atgtccatga ttctcgagta ctccatttcg
    24901 gaaggattgt caaagctcga ttcatcaaac tctggccacc gagcagttat taacaatcca
    24961 tcggaaccgg gaaggttctg ccagagctct tcagtaatgt aaggcatgaa ggggtggagc
    25021 attcttaaag aagcgtcaag agttctaacc agaacattct gagcaacgat tctaccccta
    25081 ccttctctgt tcaatcttgg cttaatactc tctatatacc agtcacagaa ttcattccag
    25141 aaaaatgagt atatcatctt tgaagcactg ggaaaatcat agccatcaat cgattcttca
    25201 actgctttta cagactttgc cagtcttgac agaatccagc ggtcctcaat ggcgagctct
    25261 tcctcatcga gattaacttt ttcaaagcct tccatattaa gcaatgcaaa ccttgcagcg
    25321 ttccaaatct tgtttgcgaa tttcttgtaa atgtcgaaaa acctaacgtc aagttttatg
    25381 tcgtgatttt gagcggccag aatcgcaagt gtaaatcgca ttgcgtctgt cccgtgctct
    25441 ctgataacgt caagaggatc tataccgtta cccagcgact tcgacatttt tctgccgttc
    25501 ttgtctctta ttaagcgtgt tatgtagaca tgattgaagg gtttttctcc catgaagtga
    25561 tatccggcca ttatcatccg ggcaacccag aaaaatatta tgtcaaacgc ggttacaaga
    25621 acacttgtgg gataaaagct ctccaaatct tcagtttttt ccggccaacc cagggtagtg
    25681 aatggccaga gctgggaaga gaaccaggta tcgagaacgt cttcgtcctg tctgatattc
    25741 tttgaaccac attttggaca gaccgacgga tcttgctctt caacgattac ttcattacag
    25801 tcatcacaat accagacggg aactctgtgg ccccaccaca attgacgaga aatgcaccaa
    25861 tcacgaatct cgttcatcca gttcaagtac acttttttcc aggtttccgg aaagaaggtt
    25921 acgctacctt cttccactgc ttctatggct ttctctgcaa gcggaccaac ttttacgtac
    25981 cactggtcgg aaagagaagg ctctactacg gtctcgcatc tataacatct gccgacggcg
    26041 tggaccatgg gttccacctt gatcaagaat ccctgctctt caaggtcttt aactatctgt
    26101 tttcttgctt catatctgtc aaggcccttg tacttaccgc cattttcatt gattcttgca
    26161 tctttgtcga ggacttcgat tacttcaagt ccatgcctca atccaatctg aaaatcattt
    26221 ggatcgtgtg ctggggtaac ttttagtgca ccggtcccaa acgacggatc gacatatcta
    26281 tcctgaataa tgggaatctc cctgttcatt agaggtagta ttactgtttt tccggctaag
    26341 ccttcgtacc tatcatctga agggtaaacg gccacagcgg tatctgcgag catagtctca
    26401 ggtcttgtag ttgctataat tacgtactca tcgctgtctt ttattgggta ttttatgtag
    26461 tagaaggccc ctttttcgtc ttcatgttca acttcctcat cggagagaac cgttgaacac
    26521 cttggacacc agtttacaat gtacttcccc cgataaatca gcccttcctt gtatagcatc
    26581 acaaaagcct tccttactgc ttcactcaga ccttcgtcca tagtgaacct ctctctagac
    26641 caatcaaccg agcagcccat ggcttctatc tgttcgcgga ttctttgtct gtattccttt
    26701 gcccaattcc aggtagtctc aagaaatctc tcgcgtccga gttctttgcg gtttgttcct
    26761 ttttttgcca gatccttttc gacggcattt tgagttgcaa tgcctgcatg atcttcgccg
    26821 ggaacccaca gcgccctgta tcccttcatt ctcttgaatc gaattattat gtcttgaata
    26881 accagattga gtgcgtgtcc catatgaaga ggggcagtga tgtttggcgg aggaatcatt
    26941 attgtgaacg gctcacccga tcccttcggt tcgaaaaaac cgccttcttt ccagcaggaa
    27001 taccactttt cttcaagatc agaaggattg tacctggtgc tcaattcctt catctaaact
    27061 acctccttac gggtttcagg catttaaatc gaattatatc acgaagtaaa accgcttaat
    27121 gggagatggc gttaagatgt ggattaggag ttaatgtagt agaatgatac gggtgagtta
    27181 tatggttgaa gaggatctgt cgacctataa gaaaatatac aaaatggtca ggatgattcc
    27241 tgttggaaaa gtggcgactt atgggcaaat tgcttccctc gtgggcaagt gctctgcaag
    27301 aatggttggc tacgccatgg ccggtatttc agacgagaca atcccgtggc agagagttat
    27361 caactcaaga gggagaataa gcattaggga tcctaacggg tacacactgc agaaggctat
    27421 tcttgagaga gagggtgttc atttcgacga aagcgatacg atcgaccttt ctgtatttgg
    27481 ttgggaaggc cccgaagccg aatctgcgaa agactggtga ttctttgcga gtctcatcta
    27541 cgccaactgt ctgcacctaa tagcacaatg taagaagtct atggtggccc ctggtgcttg
    27601 cactgcgtta tctaggaatc cttcccagta acgtgaactg tcagggtgtg atacttctct
    27661 acctcctttc cttcaaactc accgaaaaac ctgaagatga tcggataatc tccagtctcg
    27721 agcggtgtcc acgtaaagat gcagtggtcg ttttttactt cgagcgatcc ctgtggagat
    27781 aggagctcaa cattactttc tgacttccag atccacgcat ggtcaccgaa agaccacttg
    27841 tcaaagccta catatgtcca ctctgcaagt aaagagttga tcctgaattc taccttgtca
    27901 ccaaccatgg aggtgatttc tggtacggga atggttctcg agaattgctc aaaggagttt
    27961 agataaaaca tatcttccca gaagggaacg atgtttcttc cggcgctttc atatgccttt
    28021 tcaactacac cgacgcacga ataaagatct ctgtcggtga agggaagatc ccaaaatccc
    28081 gaccaggcaa aaccaaccga ccacagagct cctttctctg cagtctcgta catgaacctc
    28141 gaaatcttcc ttcggtcttg cggtgaaatt tctcccggga accttctggc tccagaaaaa
    28201 cttgttgccc agtatcttag ccaacgagac tcataagtat cgtagttgaa aaggttgaca
    28261 taagagtgat tttcaatatc atttggaaag ataaaaaccc cctcctccac caaattgtac
    28321 ccatgactaa gaaagggata agattctccg aacgtcacct cattgttgaa gccaagtttc
    28381 tcgtcggtca tagccgtttc ataatcaact ccaagatata cggcagagtg agatgggatc
    28441 cagttaggcc ctatatggta taggagatct ccttcaagta ggaacttgcc gggaagatgt
    28501 tgggccgtgt agagtgcatc tgccctagat ataagaaggc aataaggtag tttggtgaac
    28561 cttactacag tcgaaagaga atccttaatg aattgcgctt cgtatctctc ttcggtcggt
    28621 acataagagg tttgattcag gattggctgt actatcaaga cctgatctgc ttcaattccg
    28681 agagcttcgt gagagagatg aaagatcggt cctggagcgc ttgcggtacc ttcacgaaag
    28741 agaactagca aggttctctc cgatatgcgc tctatttcat agggaattga gacctctttc
    28801 tggacgttgc tgaaagaaat cttgacacct cttgttagct caaaacaatt ttcacctggg
    28861 ataagctcga ggtgtgtgtg ttcggtttca aaccggatca gtccagtaca tcctgaaata
    28921 ctcaggatga ttgaaataat caatacggta aacgaaatgc gggtaagcat ctttattctt
    28981 tagtttacca ttaagagagc gaatgggaga tcaaaggtgt ggtaaattat actgtgaagg
    29041 acaggaggcg cgatgaagag actaccaaga tcaacatctg ttgcggcggg cactatagcg
    29101 attcttacct tttcgatctt atcgaagggg atgggtttct tcagggagat gctggtcgca
    29161 ggactattcg gtacttcagc gaatctggac gccgttttcg ttgccatgac tccggcgaca
    29221 acgctttcag gtataatcgc gggtgcactt gccgcaatct ttgtccccgt ctaccattct
    29281 ataaggaatg aagacactga gagatcaaag agatatgcag gcgcggtcct tatctccggt
    29341 tcgctggttt ttttatcaat ggggatagtc tttctattga ttcccgatct tgtgattcgg
    29401 ctctttgctc ctggtttttc gagcgagata gtagcatatg cttcaagaaa gctaagatac
    29461 ttgtcgatat accctcttat aggcggactt gaaagcatat tgggggcagt tcttaagtca
    29521 aatagaaggt ttgtgcaata cggtatgtca cagctcttct ttaacgtcat tgcaattccc
    29581 gtaattctgt tgacttctcc ctttctttct gaagcatcgt atatactggc ctggatgctg
    29641 ggtaatgcga tcaccgttct gctatatctc atacaatcga gagatctttt taccctaagg
    29701 gttggcaagg ggacgtcaat tgtcgagacg ctttatctca gtcttccttt gatcttttcg
    29761 ggaagccttg gagtgataaa caacatggtt gacaaagcct tcgtatctct tcttccaccc
    29821 ggtagggttg cctctttgca gtacgcgcat acacttctcg gactgattac ctttatgatt
    29881 tctgcctttc agatgacggc atatacagag ctatcggaac ttatagtatc aggtgataga
    29941 gaaagagttg aagagcgcct gagaaagacc gttacaactt ctctgaatat ttccttgccg
    30001 cttgcagcat ggattattat gatggccgaa cctcttgtga gagtgattta tcaacgtggg
    30061 gaattcgatg ccggttcaac taatctcgtt agcatggcac ttatcggtta cggcgcacta
    30121 atagttctat cacctatagc acatacatgc tccagttatt tcacggcaag aaagaaactg
    30181 aagtccatca cattagtggc ggtcttctcg attttcttga acgcactctt cgactggctt
    30241 atgctagaac cgtttggtca tgctggaatc gcggctagta cttcattagt ggttttgaat
    30301 gctacgctga tatatgttct gctgattgca agggaaggcc tgagattcat gcctttgaaa
    30361 cggataatga gacttctcgg gtcgtcagtt gcaatgtact tgattgttct gttgctgagg
    30421 aacaacgtaa gtacttggat ttggcttctt gtaggcaacg gacttttctt atgcctgttc
    30481 ttggttagtg cgaagagtga gatgaaagcg atgatgtcaa gaatctcttt tctcttcaag
    30541 agaacatagc caaaaagaac tatgctctgt tgtgtttgtt tgtatgtttt ccaaggtttt
    30601 ctaagtgtgc ggaggaatta tcatgagaga agtggtgata cttggagccg gaccagccgg
    30661 catctttgca gcgataaatt gcacaacagc gggaaggaat gtgacgatca ttgaaaagac
    30721 gagttccgcc ggaaaaaaac tgttgatttc tggaggcgga cagtgtaact tgacaaatgc
    30781 agaatcgcca tctcttatgc tcaaaagata ctttggtgct ggacgatttc ttaagccttc
    30841 attgatggct tttgattctc aaaagctgat ggatttcttt gagcgtcgtg gactcaggct
    30901 tcacacaaga gaagacggga aagtcttccc agttacctat tctgcgggag atgtacttga
    30961 agtgctgctc aaggagtgtg acaggcaggg aataaaaata gtctataact gcagagccaa
    31021 ggacgttatt caattcccct ctggcgggtt cttggtaaag acggaatcag attcgattaa
    31081 tgcagattat cttcttattg ctacgggagg aaagagttat ccggaaactg ggtcaactgg
    31141 tgacggattt gctcttgcta agaaacttgg acacagtcta tcagagcagc ggccggcgtt
    31201 gacttcagtc acaattagga attttggttt cggtgatctg tcgggaatct ccctcagaaa
    31261 tgttgaagta tctttatgga gaaatttgaa gaaggttacg acccgggttg aagatctgct
    31321 gtttactcat gacgggtttt ctggacctgt tatcttacat ctctcaagag aagcctcgcc
    31381 tggtgacata atcagattga acttgagcgg tcagttacgc gagaaacttg aggccaatct
    31441 catctccctc ctttcaaaag aaggaaagaa actcgtgaag aatgtcttgg acgagtatgg
    31501 tctacctgaa agattgctgt cgaagatcat tgatcttgcc ggagttggtg aaagaatatg
    31561 ctccgaagtc agaaaggatg aaaggaaagc gttggttact ggattatgcg agcttgactt
    31621 cgaaatagaa cgggtcggag acttcactaa tgctatggta acaagaggag gagtttcgtt
    31681 gtctgaggtc aatccaaaga caatggaatc tagaattgtg aacaacctct tttttgcagg
    31741 tgaagttctc gattttgatg gagagacggg agggtacaac cttcaagcag ccttttcaac
    31801 cggatatgtt gccgggagga caataaatgc aagaacatct cgggtagaaa acagatgaaa
    31861 tagagcatgc aattttctac tcgaagaaga aaacgatatg ttactatgat gtcaggttgt
    31921 tggttatgct gttagggctt ttgtctggag ttgtctagaa gacttgaatt ctcgaaacgt
    31981 agttctcgta attcgaatta gagatttgtt tacgttctca atgtctcgct ttcggaggtg
    32041 cattttggaa gtaaaaaagc caaaatggtt tgacagctgg atagagatcg atgagagtta
    32101 tttgaaagtc gcgggaacaa gctggagact gaaagccaat gctccaaaaa aagtgaagag
    32161 agaataccag agacttttga agcaaattac tggaaataat gattatcctt attcatggaa
    32221 acatgagcgc tgaatcttta gaatccgtaa gctttatcaa ccggatttat ggctcattag
    32281 acagatggga gggtgtgatt tgagcattac tactggcaca ggagataaag gtgaaacgag
    32341 tctttggagc ggggagagag tgaacaaaga cagcttacgt gttgaagcat acgggacagt
    32401 ggatgaattg agctcttttc ttggtgaagc caagcatttc gtgtcggagg aaatcggccg
    32461 ccacatcgtg tctatccagc gatttctctt taaactagcc ggtcaacttg cgtcgagaga
    32521 catgcaatac gtcgaaccga tagtcgaaaa cgatattgtt gcgatcactg aacttgtgca
    32581 cgagttcgag tcggtggtca aacttgacgg atttgttatt cctggaagca ctataggttc
    32641 ttcaaagata gacatttgca gaacagtcgc cagaagagct gaaagacgca tcgttgcgct
    32701 ttctagagta gaaacgatcg ataccacgct gcttaagtac attaacaggc tctctgatct
    32761 tctcttcatc tttgcgaggt atgaagaata caaggcgggg aagattgttt ataagaaagg
    32821 cataaagtga gtttatcgat agcttaccct ggcaatacca tccttcatgc atgaaccaaa
    32881 accgtaggcc actgagctgg tgtccattcc aggaagataa aaaaccgtta tcatttcgtc
    32941 aacgtcctga ccggaggtct cccacagttt gcgggtaatt ttctggagct cggcttcgct
    33001 tacaactgat ttcttcagtt gaattcttgc ggtgcgcctt ctccaggcct ttccaaaatc
    33061 cctcacggtt acgactgaat aagcgtcttc cagattagac ttcaactcca aagtctccct
    33121 ccaaatatga tgggaatatt atacaacatg ttacaaaaca attccaactg gtacaaataa
    33181 tcgggtcggt aattacttct atacaattcg catactaaac tattaggcat cgcttcactg
    33241 aacgatggaa gagaggagta gcattccgaa cgggattcat tgcgtaatcg taagtaatgc
    33301 tcttaggaac tccaccaaaa cagcagcctt tgtaacaaaa aagagattga tactatagtg
    33361 gcgctgaagg ttcaagcttt ctttgacatt agcaagagct tcggaagctc cctagccgat
    33421 attgcttctt ccgatgccga gaactttctt acaaacgggg aagaaaagta ccacattttt
    33481 gctgaacaaa aagcctggac cctttgtttt gtagtaaagt tctgcaagtt ctgcctcgca
    33541 atgtgggcac ttcggcgtca ggtcgtcagc ttagcagatt catttccttc agctcctttc
    33601 aattcgatct aactatcgca tggcaactgt aattcccaaa cagttacctg gtctgtagca
    33661 ctaactgatt atgagtttga gatagccctt tgttctgacg agaatctctt gaacgatttc
    33721 tctgaagaga atacctcctg taagcgtttc ggtctttacc agtttgcaaa gggatatttc
    33781 gatttctctg catagtttag tagtttccga aagttcctca tagctcacgg agcttgaaaa
    33841 cattagattt ctcatcgcca aaatcccagt tgttttcgtc agagcgtctc cccaaagaga
    33901 gaactcaaat atccagggtt cgatctcacg gatcagatca gcgggtagcc tttcgtttag
    33961 cgctttttcc gattctgaga tctttgttgc ttcgcccaaa agaatttcat agacctcagg
    34021 ccatctgttt ttcgataaga gtttgctcaa gttacttgtg agtcctgtaa gtctctcgga
    34081 gtgggtagtg tgtatgggac ttcgaagatt gtattcacag aaatggatca tctcttcttc
    34141 acaacccggt gcaatctcgg agattgctct cctccaggat gagtcagaat catagtgata
    34201 agggtctaga aggaattccg atgccgagaa aattgcgatt ttcgaagcta aaggttgaat
    34261 catagggttt agaactatac cctccgcgtg attgacaatg ttcgggtctc tgccttcata
    34321 aggacctaca tgaagctctg gtaccataga agcatcgttg acaggataat tatcccacaa
    34381 caggatcttc ctttggaagg cattctcggc caactctgcg ttatctgcac tgagaacatt
    34441 agagcaaacg tcgggcccgg tccagaggat tgagatactc ctgtcgagtt ctcttccaag
    34501 tgtcttcata tactcggttg cttcgcgacc gcagtactga gtgggacaga tcataaaccg
    34561 ggaaagagtc ttgagcttct cgttaatgct tttgaaaact gagtttgtga aatatgcctg
    34621 tgccgatgag agggaagaga aagtgttccg atctttttca taaatcaagg tttccggaat
    34681 atcatcataa aaaagtccaa aggaacggac acccgcatct gccagcctca ttagcttttc
    34741 taccatggta tttacatccg aattgctgga atatgctata ttcaacccgg ggctcaccgc
    34801 aaatactatc gatattccac gggattccgc ttcctgaacc aggatagaga actctttgaa
    34861 gaagttttcg tcatattgct cacgccagcg ctttctgtgt agctcatcgt cctttggagc
    34921 atagatatac gtgttgaaac cgtgatctcc aagatactga ataacttctc ttctgatatc
    34981 cattgtccac ggtttgccat aaaagccttc aactactccc ctgatctgaa aagccataaa
    35041 tgtttttgct cctctcgttc aaactgcttt gacaatttcc tctacactat cattattccc
    35101 gatcatcgaa gatagcatca tcattgtaac gcaagaatat tctttgcgac atctcgattt
    35161 ccactggatt ttgtatctaa tggcaataat tgtagaatag tttaatgaag ggggtgggta
    35221 gaatgaaaat aggtttggta actgacaata catgtaatct gactgccgaa gagataaagg
    35281 aatacgatgt gcgcatcgtt tctctgtaca taaaccgaaa cggtaactat actaaagcta
    35341 cagagcttaa tctcgatgaa tactacgaag aactcgccgc agctgcggaa ttgcccagga
    35401 cgtctcagcc ttcacctcaa gatttcattg ctgtcttcaa ggaagccttg gagatatacg
    35461 atgcgctgat cgtgccggtg ctgtctggaa agttgagcgg aaccagtgtt tctgcaaaca
    35521 tcgccgccaa ggcctttgag aagcccattc atgtcataga ctcacagctt atcgccgatg
    35581 ggacgggaat gctggtgaag aaccttggcg acatgataaa ctctggtgcc aaccttgatg
    35641 aactagtgga atacggaacg aattttcacg aaaagaccag aacgttttgt tcgacagatg
    35701 acttgagcta ccttgtgaag ggcggaagaa ttggaaaggc gaaggcacta atgggaaacc
    35761 ttctcaagat gaaacctgtg attgttgttg acgaaggtga gctcaaaccc gtggaaaatg
    35821 ttagaggaaa caagaagctt ctggatgttc ttgtgagcca tgtgttcagt gagattccac
    35881 ctgagagatt gcgtagagct agggttctga ctatatggag gaaggaagac ggcaaaactt
    35941 tagaaaggat gttgcgcgag aaagctcccg aagccgaagt agaatcgaga gttatcgaag
    36001 caattatcgg tactcatctc ggtcctcaag gcataggagt aatcagtgaa atgaagtgat
    36061 aacggcagct gaagttcacg atgacaaaat aaggattctg cagagggatt tctaaagaga
    36121 tcggagtgac tggaggaaaa agagttttgg atgcgcttga aagaatagag ataaggtatg
    36181 gaaaagtctt tgactttcta caaagcatgt acaggcttac aaatgaagag aggcttacaa
    36241 aacgcttctc tgagctttcc ccgggttacg ctcctgtagg gaaaatcgat gaatgggtaa
    36301 agagtagccg cgcaaatcaa agaacaggga taatactttc aatagatttc tgagtgtcat
    36361 ttggaacttg cactttattc aggaatgtcg agaaattaat aagcccggaa gacctccggg
    36421 cagggtggta gcagaagaag cttttcacga tttatatgac ttaaggctac tgaactttgt
    36481 tcgtctttta aattcaaatg tggaaaacgc tttacaggaa ttttggagtt attgactgta
    36541 aatgagtttc tttcgaagtt aaaagagaga gtttacatcc cacatacaac ctagcaagat
    36601 tctcatcagg gggtgaaaaa atggaatatc gcagacttgg gaagacagat cttgaggtat
    36661 ctcttctggg gttgggaggt tttcatcttc tcgagataga gcttgagact gtgaaggaga
    36721 tcgtcgaaag atacctcgaa gcgggcggga attattttga gaccgcaagg tcttacggtg
    36781 atggagtgtc cgagagaaaa ctctctctag ttctacccaa agacgggata atagttgctt
    36841 cgaagaccga aggaagagac aggaagtctt ccgaaaggga tcttttggcc accctcaagg
    36901 ctcttgggag agagcacata gacatactct ttctccacgc cgttacaaag aatagcgact
    36961 gggaagagat atcctcctca gatggtgcac tgaggactat agaatgggcc aagagggaag
    37021 gactcataag ttacgtggga ataaccagtc atggttatgg cggaacacta ttgagagcgc
    37081 tcaaggaata tcctttcgat ctcttcatga cacagatgaa ctactatgac agattcaatt
    37141 tccctgagat agaaacaaag gtgataccct ttgcagtctc taacaacata gggatagttg
    37201 cgatgaaacc acttgccgac ggctatttat ggaggagcac cgaaaacgct ttcaggtata
    37261 ctttgactat tcccgtctcg tgtatcgtaa gcgggataaa ttctattgag cagcttgaag
    37321 aagatcttcg cctcattgag aaggaaccat tttcttctga agaacttgac gaattgcttc
    37381 taaccgctcc tgagctcggt aactatgttt gcaggcagtg catgaagtgt ctcccatgtc
    37441 cacaagggat aaatattccc ggtttctttc tcgcagaagg tcgctatgac aggcagatgc
    37501 tcgacggaaa agtgagggat gctgcagatt atgccttgag ggacagactg gctcactggt
    37561 ttggaagtga agagcagggt ctttcagagt acagtaccat atctcccggc ttagatagtt
    37621 gcgatgattg cggtgtatgt aatgaacggt gcccatacga aatcgatatt cctaggaaat
    37681 tgagaatcgt caaatcgaag ttcacggaag gatatatttg gtagtgtgtg gagatgtcgg
    37741 tgagaaagac cagttttcct agttgtgaag tagtaggctt ctctttcgta tttattgttg
    37801 gtaccttgct tcacatggcc tatgactgga gcggggacga tgctatcgta ggtatttttg
    37861 cgccggtaaa tgaaagtatc cgggagctcc ttaaaatggt tttcacgcct ggactaatac
    37921 ttctttttct agaattgctg ttttgcagtg agataccgac cgcttctttt ctctctggaa
    37981 aggcagttgg aatctacgtg atgtgcgtaa cgattcttgg aggtttgtat tcctattctc
    38041 ttgtcattca ttacggtctc attcttgatg tacttctcat ggtaatcgcg attgcactgg
    38101 ggcagctagt gtcgttcaag atctcaccca ggatcgctgc agattcaact gcaggaatca
    38161 tttcacttgt aatgttggtc ttgctaggtg cttccttcgt agtattcacc tttttgccgc
    38221 cgcattggga gttctttaga gattcaataa cgggagcttt cgggatctag cgaagataat
    38281 agttaccggt ttttgaatac agaaacaatg actcatcaga ataaaaggag gtaatgtctt
    38341 gctttcttca gaagagattt tcaaatctat ggaaatcaaa ctggaagata ctttgccacc
    38401 ggatccagaa ttcaagccgg gaatccggag agcaccgaag agagaactaa ctctctctaa
    38461 aaaggagata gagcttgctc tagtgaatgc gctgcgttac atacctgtaa ggcttcatga
    38521 gaaacttgcc ccagaattcc taaatgaact tttaacgatg ggcaggatct acggatatag
    38581 atataggcct cggggtgaaa taaagggcag gcctatcgaa atgtataagg ggaagtgcat
    38641 tgaaggtaaa gcatttcagg taatgataga taacaatctt gacttcgatg ttgctctcta
    38701 cccttatgaa ctcgtcactt acggtgagac gggacaggtc tgtcagaact ggatgcagta
    38761 caggcttatc aaaaggtacc ttgaggaatt gactgaagat cagactctgg ttgtaatgtc
    38821 tggacacccg cttggattat tcagttcaaa caaatctagt ccaagagtaa tcataacaaa
    38881 cgcccttatg gtaggaatgt ttgataatca ggaacagtgg ataagggcac aggcgcttgg
    38941 agttgctaat tatggccaga tgaccgcggg tggctggatg tacatagggc ctcaaggaat
    39001 tgtccatgga accttcaaca cgattctcaa tgcaggaaga ctgaaactcg gcattggtga
    39061 cgaaggcgat cttggtggtc atctctttgt ctcatcggga ctgggaggaa tgagcggagc
    39121 tcagggaaag gccgtaaaga tagcgggcgg tgtcggcata attgccgaag ttgaccgatc
    39181 aaggatcgag acgcgactcg aacagggatg ggttgacgag gtgatcgaag atccttctga
    39241 agcattcgct ctcgcaatag aaaagatgaa gagcagagaa agccaagcta ttgcctttca
    39301 cgggaacata gtggatcttc tcgaatacgc tgtaaataat gatgttcaca ttgaattgct
    39361 ttcggatcag acgtcctgtc atgctcccta tgatggaggt tactgtcctc agggtttgac
    39421 tttcgatgag agaaccgagc tactgagaag tgacaggaaa agatttatag aactggtaga
    39481 taaatcactc gtgaggcatt tcagtctgat aagaatgctt gttcacaagg gaacctattt
    39541 cttcgactac ggcaattcct tcatgaaagc ggtcttcgat gccggtgcaa aagaaattgc
    39601 gaagaatgga acggatactt cggaaggatt catttttccc tcttatgtag aagatataat
    39661 gggtccaatg cttttcgatt tcgggtacgg accgttcaga tgggtatgcc tcagcggcaa
    39721 aaaggaggac ttgttgaaaa ccgatgccgc agcaatgagt cttattgatc cagatcgacg
    39781 aggccaggac agggacaatt acatctggat tagggacgcc gataagaacc ggcttgtagt
    39841 tggcacgcag gcgagaatac tttatcagga tgctgccgga agacgcgata tcgctctgaa
    39901 attcaacgag atggtgagat gcggagagat aggtccgata atgattggga gagatcacca
    39961 cgacaccggc ggtaccgatt cgcctttcag ggagacatcg aacataaagg atggaagcaa
    40021 tattatggca gacatggcaa cccagtgctt tgctggcaat gctgcaagag gcatgagcat
    40081 ggtctcgctc cacaacggtg gaggcgttgg aataggcaag gcaataaacg gtggctttgg
    40141 tcttgtattg gatggaagcg agagagtgga taaaattatc agaacatcga tatcttggga
    40201 cgtcatgagt ggtgtggcca gaagagcctg ggcgagaaac gaaaacgccc tgtcagttgc
    40261 atctgaattc aacaggcaaa agagggggag cgagcagata acgcttcctt atctggtgga
    40321 tgaaggaatc atcagcaaag cgattaaaga agctgaaact gaaaaggaga aatgaagcaa
    40381 tgacgatgta cttttcaaag agtgacgaag aaacaacaaa aacactcctt gcgagaatat
    40441 ttgaaagggc cgaggagaag aatatcgata aagtgatctt gccttctacg agtggtgtag
    40501 ttgcaagaat ggcttttgat cttgtccctg attcagtccg gttgataatc gtcacccata
    40561 gtgttggctt tcgagaacca gatgaagatg aattcgaccc tcaagttagg aagctctatt
    40621 ctggaagtcg acattcacta cttacagtga cccatctctt cagaggaata gaagtagctt
    40681 ttgccaaagc gaatggcggt gtgtatcctc tacagatttt tgcgacagcc ctgcgcctct
    40741 ttggccaggg aacgaaagta gccgttgaga tagccataat ggcagccgat gcaggaattg
    40801 taagaaccga tcagtgggtg atttcggcag gcggaacaaa ccacgggata gatactgctt
    40861 ttgtactaaa gccatgtcat tcaactgatt tgagtatctt caaattcggg gagctaatag
    40921 gtattccgtc tagtctctaa tctgcatatc tgattgaatt ccttgttgat tcaggctata
    40981 atcaatttta tgtggggccg tagcacagct gggagcgcgc cgccttcgca aggcggaggc
    41041 cgggggttcg aatcccctcg gctccaccag ggggagttat ctcccctttt ttgtttctgt
    41101 ttggacagtt actcttgttc ttgcttaaaa aacctcatcg ctttcaaaag atgaactatc
    41161 gcatgaaaga ctttattttt cggaaaagag tgattgatca aatattcttt gtctcccctc
    41221 gatcttctaa agatccttta ccagttgttg cacagcatga aagttattct gaaaagattg
    41281 ctactggccg atttctatac caaacgaact ctataatcac aatgcactgc ggactagact
    41341 gtttttagag actggattag agtgtatctt cacttcagtg gaagagactc taactgatat
    41401 catcgttgtt gaggtgagaa ggtgcaagat aaagaatcga gactgttcga aagtgtccgg
    41461 aacatcgcag atagggtgga gaaatctgta cttgccttcg aagacgcctg agagagttcg
    41521 tgacggtgtt gttattaacg atgatttctc ggcttctcag cacttgatct ctcagcttaa
    41581 cgaacccaag aagcttgaaa aattatctct tcatcaaagc aatctagtaa gaattctttc
    41641 tgcaatcagg atgggtgagg ttcttagcta tgaggcatta gaaacaaagc ttagggagtg
    41701 ggagaatgac attcttttgt ccacatcgtt gttcagagga gtacaacgta gtggaagaaa
    41761 acttgcattt agagatttgc tgactgttat tggaaagtca gaagagatcg attcacttct
    41821 ctcaagagta attcgctatg attcttccga tctagttgct gcaaaaaagc atgcttcagt
    41881 ccttctcgag tctgcggtac ttgacggaag agatggcttc gatccggaag ccctgctaga
    41941 tctatggaaa gagaatgaaa gggtccgctc tcttctgctc ctgctatcag gtgagaaaag
    42001 accattaatt agggaaatga taggtaagtc tctcggagat gatctttcac ttcacctcgg
    42061 tattatcttg aaagcggtga ggtatggaat caatcttccc gataatcacc cggccttaac
    42121 tgctggtcct tgcttaaaaa agatttactc tttgcttata gatctgagag atcagattcg
    42181 tgtgaactca gttcttgttc agttcagttt ctatggtgat cctctgaaga caggaaaggg
    42241 ctccagtggt ggaatgggaa cgtttctcag aacactcgga aatgaactga ctcagaactt
    42301 accgggtgtt gttacaatcg ttccactcga aatgagggaa atatcgagaa tggaccgatt
    42361 gtggagaacg gaaagagaac atcacttctt aatatatgtt ccttttctag gctacgaaaa
    42421 gatgattatg aataacttcc ttgaagagag aactactcta atttcgaacg ttacagacat
    42481 actttcgatt gcgggaataa gcccggaaca atttcatatg aggttttcgg atttcgcttc
    42541 tcaagcgatg ctcgatctat cgaaaaagac tgggatccgg tcctttttta cggtaacacc
    42601 cgatccccag tggcgcttcg tagatcagaa agggtctctt atcaaactgg atgctgttga
    42661 tgcagtaaga gagacttcaa aagtcgaagt atcgtggaag atgcttaggg attgcgacgg
    42721 gcttttcgga ataggaagtg aggagagcag gaatcagcta cttgcatact atccggcact
    42781 tcttgacgaa agcatctcag agaaactcac gatgatgccg gaaggtatat cactacggaa
    42841 cgagtgccca atgtttgcgg atgggccact ctattcaatt ctattcgaag aagataagga
    42901 gttctacctc gaaagatctt tcagaaacaa tcctctgctt cttacggttg gaagactcga
    42961 tcctgcgaag ggccaggtga aactactcaa ggcctgggga aactctggcc tctggaattc
    43021 gtttaatctg gtgatagtcg ggggagatct gaagcatcca agtagaatcg aaacagacga
    43081 atttgaggag atcaggaaat acgtgagaga aaacaaagaa ctgcacggaa aattctgcca
    43141 cctgccggcg atgaacaacg acgagattag atgttttgaa aatagtattt cacgagtaaa
    43201 aacaactccg tggcctccta cgtacgttgc cccttccttt aaagaggagt ttggaatagc
    43261 catacttgaa gccatggctg caggctttgt ggctatcgca ccaaaacgtg ggggagtgaa
    43321 gagctacata cgggacggat acaacggttt tctcattgat acgacagacg aggtatcgat
    43381 cgaaaaagag ctaaggagat tgctgataga tgcaagaccc tctgccagaa gaatgaagat
    43441 gattgccgag aatgggagca aaaccgttta cagaaggttc tccatttcca ttgttgcaaa
    43501 gcagttttca tctttatata cttcttgagt ggtttttcca gcgagtttgt aatagaaggt
    43561 aattgatgca aagaataact tcattttcaa gtgagcaagc atagtcttca gctttagaag
    43621 ctctatcaga gatcttgatt caagtataga agatcttggg ttggcaagac aagatggaca
    43681 gatagacttt gaagatgagg actgtgtgat aagattattt atggaccact gcgggataag
    43741 ttgggtttcg gtcatcaaca gattactaaa ctttccaacg ctaacagatg tttgtctttt
    43801 gaatgcttct agcgaacggt gctcttgaat tcaacagaag aaatcaacga ctggcccatc
    43861 gataactttt cccggacaaa tcgatttgca aaaatggtta tcggggtatc aagtatccca
    43921 gtaaaaaagg aagtggagat atgagagttt taatggtcta tccagaatat ccagaaacgt
    43981 tctggagttt caaacatgcc ctgaaatttg tctccaagaa ggcggcgtat ccacctttag
    44041 gactaatgac tgttgccgct atgctgccta aagagtggga gaaggaactc atcgacatga
    44101 acacggacat tctacgtgat gaagatatag agaaatcaga ttacgtaatg atcagctcaa
    44161 tggatattca gttcgactct gcaaagaagg ttattcagag gtgcagagat ttaggcgtca
    44221 agactatagc gggcggacca cttttcacaa caaggcccga agaattcagt gaggttgatc
    44281 acctagttct cggcgaagca gaaatgactt tggcaccatt tctccttgat ttggagaaag
    44341 gtcgagccaa gcatatttac aaatccgatg gcttcccaga tattggaagc agtcccattc
    44401 cagactggag tcttttaaac atgagaaagt attcttctat gaacatccag tactctagag
    44461 gctgtcctta caactgtgag ttctgtgaca tcgttctgtt gaacgggcat attcccagga
    44521 caaagagcgc ggaaaagctt atctccgaaa tggaagcttt gtataaggcc ggctggcgtg
    44581 gaggagtatt tattgttgac gataacttca tcggtaataa ggcgaaactg aagaaagaga
    44641 ttcttccggc cattgctgaa tggatgaaaa acaggaatta tccgtttgtg ttgaacacag
    44701 aagcctcaat agatctttcg gacgacgatg aacttatgaa actcatggta gatgcaagtt
    44761 ttggaactgt attcgttggg atagagacta ccgaagagga aagtctagtc gagtgcggta
    44821 agttccagaa ccggaacagg gacttgctat catctgtgaa gaagatgcag gagttcgggc
    44881 ttcaggttca aggaggattc attgtaggtt tcgatcacga caaaccctcc gtttttagaa
    44941 acatgataga tttcatccag aagagcggta ttgtgacagc aatggttggt gttctcactg
    45001 cgccaacggg aaccagactc ttcttgagat tgaaaaaaga ggacagaatc gtatcggatt
    45061 tctccggcaa caataccagc attatgacta acatagttcc caagatggga atgaagaatc
    45121 tagtagacgg atactacaag attctcaata gcatctacgc gccgaagcca taccgccgga
    45181 gagtaataac ctttcttaag aactacaaac caaattcaat taagcacgtg aggcctttcg
    45241 ccgatagtgt gaaggccttt ttgaagtccg tatgggtact tggaattagg gataagggcc
    45301 gaatgaacta ctggtcgctg ctcctgtgga cggttttctt caggccgtca cttttcccat
    45361 tgtcagttga atttgcgata tacggctacc acttcagaaa gtctttttca tttgggatgc
    45421 tcgtggaaag cgatactcaa tcgcctagcg cctagaaaga attcatgtag ggctaaattg
    45481 gaaacgcaaa tcagattgaa gacgagttta gacctctgtt ctcaagaggt tataattgta
    45541 ttcatgccaa tatcctttac aaagaggata tcgaatattt atggcaattc tctatggcgg
    45601 gagtgagaac atagttaatg tcaagaaaga gtgtaaaatt cggtagcttt ctcagagatc
    45661 atatagatga cagagatgtg ttcagaaaat ggcttcagct cggcatggca tttgagaact
    45721 tcagaagaag gcattttcca gacagagatc gaccgcgatt tgttaatacg gttaatcatt
    45781 tggccttgga agaggtctat ttcgcagtga agggtgagaa gaccgtttgg gtaaatctga
    45841 tgaacccttc ggagcttctt ctttcggcgg ggctaaaccc cgtttcggcc gaaggcattt
    45901 caggcgcact ttcttcgatg catcttgagg acacggcaat ctcagtttct tcacaggctg
    45961 gaataagcga ttctctttgt acttttcata gggcttctct cggggtctct ttgagaaagc
    46021 tacttccccc gccgaaacta gtgatgacca caagcattct atgtgacgga aatctcccga
    46081 cctttaagag aatagctaga gaatacgatg tgccttttct tctgatcgat gtacccagag
    46141 gtcggaagca ggaggctgta ggatacgtct tgtcgcaact caaagatgtg attgtcacaa
    46201 tcgaagagat aaccgggaag ccattcaaga tggatgagtt gtcattgaga ttatcttatg
    46261 aaagagaact tataaatagt ctagtaaagg caaaactcga ggcgcgcaac tcgtatcttc
    46321 ctcagcatct ctacgaacat atgaattcgc tgtatgtcct gcatactctt gccggtgatc
    46381 ctcgaattgc agatggctcc agagatatcg tcgagagcct tgaagtgatc cccgaaaacg
    46441 caaagaggat actatggcta cacataccac cttattacga caatgagcta ttcgagctat
    46501 ttgctcctaa cggtgaaaat ctcgttgtag gaaatgaact tatgtgggac tggatatatc
    46561 cagtggatcc cgaccgaccg ctcgaaagcc ttgcaaaaaa gcttgttttc aatccgcttt
    46621 gcggtagcgt cgttgagcgc ggtgagttct gtttgggtct cgcaagagat ttcaatgtcg
    46681 atggggttat tcacttttca cattggggtt gtagacagtc ttccggtggc gtacctttcc
    46741 tcaagaggtt gtttgaaggt aagaagattc catttctaga gttaacagga gactgtgtcg
    46801 atcattcatc acaaggtgcg ggacagcttc gaacaagagt ggaagccttc ctcgaaatac
    46861 tggagaatag aagatgatct actacacatg tagctatata cctttggaag tacttatggg
    46921 aagcgacatt gagttccaga ggctgatgag atcttctccg acctcaagtc atgaactggg
    46981 ttgcaacctc tgtggatatg caaagacagt ttatcagaag ggcatggagc ttgactcgaa
    47041 tgattgtctc ttgatagtgg attcttgcga tgcaatgcgt agagttggag atcttctcga
    47101 tgaacactcc ttggcaaacg tattcatcct cagattaccc tggaaaaggg gtttagaagc
    47161 agtgacattc ttatcaggcg agattcaaag attggtttcc tttctcgaca gttctgctat
    47221 ttccatcgat ctctccgaag gtgttcgcag atttaacaaa cttgttgatc atgtgctcgc
    47281 aaatgaaaag gaattggcag caggagacct tacggatcta taccttcatg cacttaatgg
    47341 caagcaggga agttttactt cgaaaagcgg agcaactgca tgcacgagcc gacttgcaat
    47401 aaccggcgga ataactgaca ttgtggcctt agatgaagct gttaagaatg cgggaggtgt
    47461 aatctctgtc aacgacacct gtttgggagg tagaccattt tcagagagaa ctcaagagaa
    47521 acccgatccg tttcaagcag ttgcagagag actccttaaa tggagatcac cgtgtggtcg
    47581 attctcggaa ggtgagtctc attcaaatag ggcagttgat gcgacggtct ttgtggctcc
    47641 gaagttctgt gatttctatg actttgtacg tcctaaggat ggaaaaagtg tctacagaat
    47701 agagcttgat tatccaatca actcccaagg acagttgtca actagaatag gagcgttaat
    47761 ggagaagaac agcgtaagat cagtatcacc ttccaaggag gaaaagaaga tgcttttcgc
    47821 cggggttgat tccggatcta ccacgactaa cggagttctg atagacagag aaggaagaat
    47881 aatttactcg aagacattga agaccggtgt aaaagcctca aatactgcgg aagctcttat
    47941 acaggagatg actgaggtgg ccttcaaaga aggcaaccgc ataggaaaat gtgtttccac
    48001 tggttacggg agactgctgg tatcatctgc aaatgacagg attacggaaa tctcatgcca
    48061 tgccagggga gtcttcgaac tcttccccga ggcaaggggg ataatcgaca ttggcggtca
    48121 ggacagcaag gtaatccggc tgtccccgga tggtaatgtc gatgacttcg ccatgaatga
    48181 caaatgcgct gccggaacgg ggaggtttct tgaagtaact gcgtctgcac ttgaacttgg
    48241 aatagatgag atggccctct tggcaagaaa acggaacaaa gacatctcta taagttctgt
    48301 ctgcacagtg ttcgcagagt cagaagtcgt ttccctgatt ggaatgggag agagaataga
    48361 ggacatatcg gccggtctgt ttagggccat tgccaagaga gttggagcga tgtattcgag
    48421 acttggatct cccgttcccc ttgttttcac cgggggcgtt gcgaaaaact ctggagtagt
    48481 tgacgctttg aaggaacttt tcggaacaga catcataatt cccgaagtac cagaaatagt
    48541 gggagccttt ggagctgctt tgatcgcaag agattcggta gttgaatagt agctttgatg
    48601 aacttgtgag cttcactcaa tattgcttct tcacaaaact tgggaagtga gccaatggat
    48661 tttgatgatt aactgaagaa agttccagag atcagagaag gtttattgag gatatttctc
    48721 atgtcaaacc ccacgaggac aaatgggtag tcgaccatga aagtgaagag aagatgattc
    48781 atgtgttcga agagaaggtt gatgcagtta aggcggcctc agagatggcc aggaacagcc
    48841 cgataaaaga ctgtgattca tgattcaaat ggaaacgtgg aaaaacaaaa cggtgtgaat
    48901 tggtaagtct cttcaagaat gctttggagc tacgagagtg tttattcctg tggcacttca
    48961 taatagattg tccagacact gtaagatttc gcaaaaactc tgttgttttg gaagaaagaa
    49021 ataaactgcg caacgaggcc actctatctt gttcaaagca aagttcaatc catttgaaaa
    49081 acaagtatgg aaatccagga gtcatcagaa tgaagcagcc tcgagatagc agacgttaat
    49141 cttagaacac caaaacatta cctgaacaac aatgaaacaa gatcatatct tccctggttg
    49201 gaattacaaa tgcgtctcag agcggaaaat acacccttgg gcggaagcga tgagtaaaaa
    49261 cgagataaga gtagtcgttt tatactccgt gagatctctt aaacaaagcg gttttcactg
    49321 ctaccctaat agttttcaat ggaacatttg cgactgagtc accttttatc tattttgaat
    49381 acccactgcg caaagactcc tgattcaacg attacagtat gtgctagacc ggctggaatg
    49441 agaagagatt cgccaatttg aagcgtatgt tcttcattaa ctatcatttc gtcgcttctg
    49501 tactctcctt cagaaatctc atccaccttc cctgtgagtt caccggtctt gacctttgcc
    49561 tttcccgaga ttactacata tagatcgtgc tgattcctgt gtgcttcagg ccttccagaa
    49621 aacggggctt tgagctctac tgctttagcc gagaagccag ccactttttg cgcgaaatca
    49681 agactgtttg ccgagtattt gattgccctg tctgccatcc tagtcctcct tttaatatca
    49741 aacatagtta tggtccttga aaactactgg ttataaatct ccaaactctc ctgctacagg
    49801 cgtcgaaaga tagtttccct ctaagataag tgggatgaac gagttgttct cttgaggctc
    49861 gacgttcaaa ttaccgccga gcaagctctg aagtttttca acctgctcat gctcaccaag
    49921 caccatgaga ctgtctcccg gctttacaat agttgatgcg ctcgggttga atttgttcac
    49981 accgtcgcag accgttgcaa tcacaatcaa cccaatcctt tgaggaattt tcaaagcttc
    50041 aagcgtcatg ccctctggtc tgaaattgcc tggcactttc acttcttcga agcgaagctg
    50101 aagcgaaccc gattgagata tcgtgtcaag aaagccgatt acagttggat taagcatcat
    50161 cgtaaccatt cgatgcgcac cgatttcttg aatgggaatc actttgtcgg ccccggcata
    50221 gttcagcttg ttgatgttct cgcgatgggc aacttttgag attatcttca tgctcttgtt
    50281 cattcccctg gcggtgagaa caacaaaaat gttgtccacg tcgtccggta aacatgacat
    50341 caaaccttga gccttctcta ctcccgcgct ttttagcgtt tcttcttcag tggcgtctcc
    50401 agaaacatag atcagctttc ctccctctcg tgaagtcaaa tctttgagcc tctcttcgct
    50461 cttctcaaca attaccacat cgttatttct cttcgacatc tcgtgagcga tagtcgaccc
    50521 cagatcacca ccgccgatta ctataaaatg attctcaagc ttctctatgg ttttcaaaat
    50581 tcttctcctc ctgatcattt tgtttaccct accttcgacg agaaaggcag ttatgttgga
    50641 tacggcgtaa acaactatgg tgatactggc aagaatcact atgcttgtaa agatttttcc
    50701 tgcctgagaa agttcggtaa tcgtcccata tccgacagta gagatcgtta tcattatcat
    50761 aaatatcgaa tcgatcggac tgtacccttc gattatcgag tatccgataa ttccaaccag
    50821 aaacactgct gccagtagaa tgagagagac tacgatctgt tttgcaccgc cagtttcgtt
    50881 cgtcatgatt tcctccaggt agaaagtgtc aaagtgaagc cacttcgccc ttatggtaaa
    50941 atcttatcag aagggggccg ctgtcgtcga tgaagaaata tactctcaaa agttcagaaa
    51001 ttcccggatt tttaaccgaa ggactagacg aagagcagtt gaaggctgtc attgaatcaa
    51061 gcggccgttc tttaatagtt gcaggaccgg gttctggaaa gacaagagtc ataacttaca
    51121 aaatcgctca tcttgtttca aattcaataa acccccaaaa cattcttcta gtgactttta
    51181 ccagggcggc ttccagagaa atgatcgatc gcgcaagacg tgttagcgga tcgaacctta
    51241 agggaatgct ttcgggtact tttcaccatg tctgcaatta tttcttgaga aagtatgcta
    51301 aggccgctgg cttagcggaa aatttcacga ttcttgacag agaagatgcc aaagacctca
    51361 taaaacactg tagaacagaa ttactagaag agagaaaggg tatcaatagt tcgactcttc
    51421 cttcagcggg cgtgctacag agtatctact cgtattcggt taatgttctt tcatctctta
    51481 gagagtctac agcgagaaag aacaggaagt ttctcggctc ttatgatgag attgaagaga
    51541 tctggaaacg gtacgttcaa gagaagactg ttcagaactg tgtcgactat gacgatcttc
    51601 tccttaaagc gctaaagctc ttcaacgaca atccatcgat cttgagcaaa gaggcggaga
    51661 ggttcagatg ggttctcgtc gatgagtttc aagatacgaa cgtccttcag ttcagactcg
    51721 ttgaaatgct ttcctcggtt catgggaacc ttattgtcgt gggtgatgac gctcagtcca
    51781 tctactcatt ccgtggtgcg aggtttgaaa atgtctatga ttttctttcg gctaaagatg
    51841 ccaggatcta caagatacag acgaactacc gttcaactcc tcaaattgtc gaactgataa
    51901 attcgatagt tcctcaggat tcaattgaaa aacagctgag agccgttcgt atgaacgggc
    51961 cgatcccggt tgttgccgag acctgggaca atcttgagga ggcatctttc gttgcccaga
    52021 gaattcaaga acatatagat gacggggtag atcccgaaaa catcgccgtc ctgtatcgtt
    52081 cacactatca ctcccttgag ctacagatgg agatggacaa gcgaaaaatg aatttcactc
    52141 tctactcggg tcccagattt acagagacgg cccacgtgaa ggatattctc gcgttgctca
    52201 aagtaataga gaatcctctt gatcaagttt cctggggtag gattttgagg ctatttcccg
    52261 gcgtgggaaa tgcgatctcc ctcaggataa ttcagggtat ctcagcagct gccaacgacg
    52321 gtcacagagc tgaggaggta gtaaactccc atgtgagcaa acggataaaa ctcgataaac
    52381 tcgtgagtat tttgggtgca acgaatgaag aggaacctcc aacggaagtc gttcgaaggt
    52441 tttatgccga tttttacggt gactatctcg acgagaagtt tccagatgct agggagagaa
    52501 gaatggatgt tgaaagactt atagagatcg cgggccgcta cagttccata tccgatctgc
    52561 ttgaggatct ggctgtcagc gaaaagatag acattgagcg cgagtcggct gaaaaacaac
    52621 cgtcggttgt actttccact gtgcatcagg cgaagggtct tgaatgggag gtcgtgttta
    52681 ttctagcggc caatccaggc gatttcccaa attcaatggc aatattagaa ggttctctca
    52741 gcgaagaaga acgaatattc tatgttgccg ttactagggc gaaagattat ctttacatac
    52801 tcaggcagaa gggcgggagg tcgagaccta tgatagggaa cagatacgtc tttagaagcg
    52861 gtcatgattt cgtagagaag ctccctaaaa actgcttcga aagatgggat gtaagctggg
    52921 attcgtaaag gagacaattg ttccaatata gtagcaaagt aggagggaga agaaatgaac
    52981 tcactgtact taccactttt agcagggatc atatctacca tctttgcttt cgtcatgttg
    53041 cggagaactc ttgggttttc gcagggtaac gacaggatga aagagctatc taagtacgtt
    53101 caggaaggtg cctctgcttt tttggaggaa gaagcaagaa agatattcct tgttgcggta
    53161 attcttgcgg ccgttctcgg gataattttt cagtcgttca agtacccaat agtccttctc
    53221 tttggagccc ttgtctcgga agctgcggga gtaatcggaa tgtatgcggc cacgagagcg
    53281 aatgcaaggg tagcagccgg ggcagagagc ggattgtcaa gtgctttcaa agttgctttt
    53341 tcaagcggat ccgttatggg tctcgccgtc gctggtttct ccttgacggg tttggcactt
    53401 gttatgctgg ttttcaaaag ttcctttgtt tttgaaggta taaccgacat ttctaaagcc
    53461 tttggaagga tctcttacat agacggtgta atgatagtca gctcttattc actcggagcc
    53521 agtctcattg cgttgttcga tagggtaggc ggaggcatgt acactaaggc cgcagacatg
    53581 agtgccgacc tagtgggaaa gatcgaagag catatcgaag aggacgaccc gagaaatccc
    53641 gcaacaatag ccgataacgt aggtgacaac gttggcgatg ttgcaggttt gggggcggac
    53701 attctcgaat cttatgtggc ttcgattgtc tctgcaatag ttcttgctat tttcatgaag
    53761 tttgccgacc acggaactat gacagagacc cagtattatg ggctgatatt gcttccggtc
    53821 ctgatagccg gcgcgggtgt cctttcttca ctgattggtg ttcttgtagt tgcgaacttg
    53881 aagtctaagg atgcccgcaa gagtctcagt tttgggaatc tatttacagg cggtcttgtc
    53941 ctggcatcgg tagcgatagt tatagggatc gcagcgcccg attatccatt caaagatgca
    54001 ttcttaatca atcccgaatt cgtttcacgg tggagactat tctttgcaat tgcttccggc
    54061 ttgattgcag gagtaatcat ctcaaagctt agcgagtatt acacatccga tgactacaaa
    54121 cctacaagaa aactcgcgaa agacactcaa agtggagtcg cgataaacat caccggcggt
    54181 cttgctcttg gtatgggcag cactctatgg ccagtgataa ccttagctat tgccatactt
    54241 gcggcatatt tgtctgcagg ggtctacgga atagccatag cagccctagg aatgctttcc
    54301 tttgtcggat atatcgtttc tgttgactct tacggcccag ttgccgacaa cgcaggcggt
    54361 attgctcaga tggcaaatct ggatccaaag gtgaggaagc tcacagaccg actagattct
    54421 gtaggcaaca ctactgcggc catagggaag ggctttgcaa tagggtctgc agcttttgct
    54481 gctctttcac taatagtatc ttacatatgg ggttcggctg ggacagcgga agaaatcata
    54541 aacaatccag taattaatct ggtagaaccc tatacgctta tcggaattct tataggagga
    54601 atgctcccct tcttcttcac gtctcttttg atcaagggag tggccgatac agcaaatctg
    54661 atgatagttg agattagaag gcagttcaag gaaactcctg ggattaggac tggagaagcg
    54721 gtccctgact acaggagatg cattgaaatt acgacaaagg gtgcggttag cagaatgctt
    54781 actcccggag ttgtggcgat cgcctcaccg ttcattgttg gattcctttt cggtaggtcg
    54841 gcggttgccg gtttgctagt tggcggactc tccagtgcaa tcatgatagc tatattcagc
    54901 gcaaactccg gaggagcctg ggacaatgcg aagaaattca ttgaaaccgg tgagtatgga
    54961 ggaaagggta cgccgactca cgaagctgca gtcgtaggag atactgtggg agacccgctg
    55021 aaggacaccg ttggtccttc tatggatatt cttatcaagt tgatgtctgt agtttctctg
    55081 gtctttggct ctctcttccc ggtaacacca ttctttatgt aaatagttat gaagttggcg
    55141 cggtcttcaa gaccgcgctt ttttcttgaa gtacctctgt caaagttgca ataccacgtg
    55201 ttagaattct ctcatggaca caaagaaagc ttttagcctg atttcgtctt atggtttctt
    55261 tctctttggg tttacatcaa taatactcgg aagcgcattg ccagcaatcg agaagagctt
    55321 tggcttagac catcagatgg caggcgtttt gctttcgctt cctacgcttg cctttatggc
    55381 ttcggctatt gtcgtcagtt ctctctcgaa tcgatttgga ccctttaaat tgcttgccgc
    55441 agggattgtg tgtattgcta caggattgac cgtcctctct gttggtcggt cattctggct
    55501 tttgttagta ggaagcatga cagtgagctt cgggactgga agtatggaga cttcaattgg
    55561 aatagcagtc tcaggactga actacaaaaa gcctggggga gctttgaacc tcatgcattc
    55621 attctttgcg ataggttcaa ttatctcgcc tcttatcgtg gcggcctttc ttgttgacta
    55681 cacgtcatgg tggaagccct ttctcattgc tctcttcgta tcgcttctgc tgttatcatt
    55741 cattccgttt ctttacggga taccctttgt aggaagtgat gaaaaggcga ctcacttcag
    55801 aaaggagatt ttttccagga agatcttctg gcttattatg gtgggagttc ttctttatgt
    55861 gggctacgag attggtttca cttcatggct ttcgagcttc atgtttgaat cgaagggtat
    55921 tgagatgaga tacgctagca tctttccggc gcttctttgg gcagggatat tcgtcgggcg
    55981 attactggcc gggttctttg tcgataaact tggatacgag ctgtcgcttt tgatgatggt
    56041 gataatagct tttgtctcgt ttggagcagc gttgcttttc caatcggtct ttgcgattgc
    56101 gttggcggta gtatttgcgg gttttggctt ttcgggaact ttcccgacgc ttcaagctat
    56161 cctcatatcc tccatgaaaa atggaatcgg gttcgcaatt ggaatgttca cagttgcggc
    56221 atcaattggc ggcgcaatgt ctaattttct cgtggggctt ctaggccatc acttcggtat
    56281 gatggccggt gttatcttca tcttgtttct catctttctt gagatggtcg ttgtgctctt
    56341 aataatgaga gtcaggaagg agagactcga tgcacagtaa ggtaatagtt gttggagccg
    56401 gtcccgctgg actcgccgcc tcaagaatct ttgcccgtga cggaatagat ttcctccttc
    56461 ttgattctaa aagctttcca cgagaaaagg gctgcggagg gggtttgact ccaagatctt
    56521 tgaagagggt ccacgagata tttcccgaag tggacatcga gggcttcgac tgtcacgagt
    56581 taagcataaa caggataatc gacggacaga tgaagcatct cactactctg atgtcaaaga
    56641 agacaatatt caaaactgtt gacaggaagg aatttgataa tgctcttctc aaatccatag
    56701 ttgagagagg cggtagattc ctttcgtcaa gagtgaaaga gatcgtgaaa gaggaacaag
    56761 gttttgtcgt taagacagaa gaaagagagt tgacctctga ctatgtaatt gtttcggccg
    56821 gcgtttttgg ggcaaaactc cttggacatg aaccaccctc ttttggagtt gcatattatg
    56881 gttacagctc tcccacggga agtgcttccg tcacctttat ccccaatggt tacctttgga
    56941 gtttccctgg tgagtctttt gactctgttg gaggtggggt ttaccccgat tcggagaaga
    57001 ctccctctta tgaagagatg agtaccttga taaagagatc tttcgatgaa gagattgatt
    57061 tgcggggggc accaatccct actttcaata cagatgtggt tcttagtctg aacaacatat
    57121 acacgaattt gctggttgcg ggagacagcg cgggacttgt agataactgg acgggcgccg
    57181 gtatcgattt cgctttcgat tctggaagac aggctgcact ctcgatcatt gaggatggtt
    57241 acaaaggcaa gactgttaac gaaagatatc ttgaaaggct cttcccggcc gccaggcatc
    57301 tgcaaatagc agacgccttc agaaagaggt tcaacaggaa tttcgaagaa catttggatc
    57361 ttctcaaggg aagaatgact ggaaaactcc tttctaacta tctgagctac tacgttaagg
    57421 gtccttacaa actcgcgttc aaaagcctat tcactcgtgg aatctcgagt cgctaactac
    57481 actgctgaag tcacccgaga aacgatattt ctcatcccgt cattaaccac ttcgccttcc
    57541 atcaatctca tcagcctttc tccataagaa ttagcgatat ccatattgtg tgtgatcatg
    57601 attatcgtga tgtcatattc tctattaatt ctttcagtca actccatgac tctggaagcg
    57661 ctgtcagggt ccagagcagc agtgtgttcg tcgagaagga gaagtcgcgg tttcgacaag
    57721 atagccatca ccatggccag tgactgcttc tgcccgcctg agagctcacc tgccttggtt
    57781 ttcaatctgt cttcgagccc cagtcccaga ttctttagca acgctattgc ctcttctttg
    57841 actgaactga acttgaaagg cctatttccc ttctttgaag ctattatcag attttcggca
    57901 atggtgaggg ccggaaatat gcctgtgttc ggatcctggt aaaccctacc gatgaactct
    57961 gcaagtttgt gtggtttcat gtaattcatg gtcttaccgt cgatcatgta gagaccggat
    58021 gtcggctgaa cctcaccgag tatcacctta agaagtgtgg attttccagc gccgttaggc
    58081 cccacgaccg tcacgaactg cccctctaga atcgtgagac tcacattctt cagtgccacc
    58141 ctttcgtttt ccatccctgg attgtatgta acagtcagat ctttgaattc gaccataacc
    58201 tcttcacctc ctgcttcacc tttcgtttac ctccacttga agaaagctga cgcaatccaa
    58261 taaccgtaat aataaaaact gctgtgagta gtttcatgtc gcttgcaaga aagccgatcc
    58321 tatatccgta tctcatcgct agggagagaa gtgcctgata gatcactcca cccactattg
    58381 gcgaaagcag gccgtagaaa agctttact
//
LOCUS       DAOQYI010000010        56959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_586_length_56959_cov_70.411623, whole genome shotgun sequence.
ACCESSION   DAOQYI010000010 DAOQYI010000000
VERSION     DAOQYI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 56959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56959
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_586_length_56959_cov_70.411623"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKL93_04665"
     CDS             complement(<1..874)
                     /locus_tag="PKL93_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70587.1"
                     /translation="MRKTNRKRGLALAGLLLGIGVLLIGAAVIIRPEILGFSFGSGWS
                     FNGVKFNEDFSETVTEKINSLEIDSQNGKVEVVGWNRDYVEIKVERSIRVADENLKEE
                     YLEKTRPVINAMDGKLTVIVPKLSQTNEFRGQATTIYLSVPSETVEDIAVNTSNGAMV
                     FRSLNASIRGRSSNGGLELISVSGSITLETSNSPIFVEECAGVMELKSTNGAFEANGI
                     SGTFIIETSNAPITIKKGTISVSANSTNGAITVTESTLLGDRNIFQTSNAKIVCDAIL
                     PDFGVLELLSTNGSI"
     gene            1251..3044
                     /locus_tag="PKL93_04670"
     CDS             1251..3044
                     /locus_tag="PKL93_04670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNQ70588.1"
                     /translation="MTEKKYSIEELMENETIASFAASPDYKRILYSSDKTGNYNIFEL
                     DKEKGEHRQITSLSENALVSHVYEDGSFIFAMDKGGDELHHLYLGTEEEIKDLTPFEK
                     TKSGFHVSVGNRVYYYSNKVDRSRFDLYRFDKADLASELVFENKDLYELGPISHDERF
                     LALQKPDTANNSNVYLHDLLSGETTLLSPHEGEANFKPLFFSRDSMKLYYLSDIEGEF
                     MTLWVTDVGSKEHEEILAFSWDIIAGRISENGESAVLIVNRDGFSELHIIDTKDSSPK
                     MSPIETGGVVYDQCFSKDSRFLYYLCDSATSSPNIFAIDLETKETEQLTDSMSDEVNS
                     NDLSEAKVLRFISYDGLQVPGIFYPPRNVPPNEKAPAIVWVHGGPGGQSLPRYSPEIQ
                     FIANHGYAIYAVNNRGSSGYGKSFFKAADHKHGEADLDDCVAAAKYLATLDFIDEERI
                     GINGGSYGGFMVLAALAFRPKEFKVGTDFFGVSNWVRTLKEVPAWWKAIKDLLYTKIG
                     NPFEEEEYLKSISPLFHAEKIERPLLVLQGVNDPRVLKVESDEIVESLKRNDVPVEYV
                     VFEDEGHGFKKKANRIRGANTMLTFLDKYLR"
     gene            complement(3848..4672)
                     /locus_tag="PKL93_04675"
     CDS             complement(3848..4672)
                     /locus_tag="PKL93_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNQ70589.1"
                     /translation="MVRIVTDSSCDLPVETLKKYGIPFASLNISVDDMTFKEDIDITP
                     EEFWRLMGKSRELPKTSQPSPADFAGIFEDIQKMGEIPLCITISSKLSGTYQSAVLGA
                     EMSGGKAIVFDSLAGSISHGIQVLMAARMAESGAKLGEIVEALEEYRKNVKIIIPLAT
                     VENIVKGGRLSKFQGSLVNILNMKIILHGINGEVKLLKKVRGSRRFREAIIELIADAS
                     KSGKKIFGITHVNNLEEAEFFASEIRKRVLDSEVIIGKMGPAIATYAGEGGLILAL"
     gene            complement(4862..5425)
                     /locus_tag="PKL93_04680"
     CDS             complement(4862..5425)
                     /locus_tag="PKL93_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNQ70590.1"
                     /translation="MKRVFSAVLMVLIIGSMSLASGSMTEEDGIAFMREEEKLAHDVY
                     TVLHEIWGLNVFGNIARSEQTHTEAVLSLIGDFGMVDPVGENEIGVFTNSTLQELYDE
                     LIESGSKSLLDAVKVGLLIEEIDIKDLEELLEGDIDSRTARVYENLLRGSENHLRSFL
                     RQYERLAGSYAPEVLDVERFNEIASGR"
     gene            complement(5693..6451)
                     /locus_tag="PKL93_04685"
     CDS             complement(5693..6451)
                     /locus_tag="PKL93_04685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ70591.1"
                     /translation="MIILKRALVTGASSGIGEVFSYELAKRGYETIIVARRLERLQTI
                     SDQIERDLGVRSIPLKGDLTNENDLVEVSKQLKDVDLLINNAGFGLIGPFEDIEADRE
                     MQMIKLNIGALYFLTKKYALIRNSIGGGIINVASTAAYLPVPGMAVYAATKAFVLSLS
                     EAVSEELQPKGFKVMVLSPGPTRTEFFDVASGGRRQLPGSMSPEEVVKSALDAFEKGK
                     RSVVSGLINKIIASGSRLVPRAVALKAARRAFED"
     gene            6557..7120
                     /locus_tag="PKL93_04690"
     CDS             6557..7120
                     /locus_tag="PKL93_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70592.1"
                     /translation="MKKVAVILVLVLAASVFASWVPLQDYSTVEYVYNKISYFENGEQ
                     KEMIYGVTIDRDENHFILNYDTTYFLPLDTELTSDMMFGQQFSMMMYIFLNPMLSFIY
                     DAIDLEEQMNTKLYGFGTLKYEGLHSVEGKNGTYTGTKVALYNEDGELSMYWIIDPNI
                     PFALETYMGDDYADDSTTITLWDYAIN"
     gene            7298..8359
                     /locus_tag="PKL93_04695"
     CDS             7298..8359
                     /locus_tag="PKL93_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNQ70593.1"
                     /translation="MDLARIRHDLHEIPEIGFNEFKTQAYIMSFLDRLSLSYEKVAGT
                     GILAKWKKVEGPFVLFRADMDALPVFEETDAPFKSAHEGFMHACGHDVHMTILIGLIE
                     RIVTSDLNRNFLFLFQPAEEGGGGASKCLSKLDQYEITEAWALHVNDEFPEGSVYTRA
                     GVLFASAFEVDCTFEGRSAHVAFYKQGRDAIEGAMDFLQRVYSVDRGDSVLRFGLIQG
                     GRVRNVVADSCTLSGTVRTKAFELSEEAIMELEELGIEVATKRGLKFSQEIGSKYPQV
                     LVDRNLYDKLCALVDVNPVEMKYVGEDFGVIAMKYPSVLFWLGTGRGEQHGLHNSRFL
                     PADSVIEKGVEVFWKVANS"
     gene            complement(8361..9065)
                     /locus_tag="PKL93_04700"
     CDS             complement(8361..9065)
                     /locus_tag="PKL93_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ70594.1"
                     /translation="MRELLSLLKRTGCLTDPRLESALMELDRRDFVSPEVADEAYSDR
                     ALLSLASSGEICSTSTQPSLLISMIEELKLHEDNKVLDLGTGTGFCACLLGKMLTMGS
                     VVSVETSRTVASIASENLRRYRVNNVQIVIGDGRCGFKEEAPYDAMISTVAFPGITVE
                     LFSQMKIGARSIVPIHRSYMNTPVFLFEKTGEVTLRAEIRTGAVFMVVEDSKPDPLYS
                     DNKFSLELREGKFRRL"
     gene            complement(9087..9818)
                     /locus_tag="PKL93_04705"
     CDS             complement(9087..9818)
                     /locus_tag="PKL93_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNQ70595.1"
                     /translation="MTVLQFVIIYVAGAVAGFMNVMAGGGSMVTLPVLMWIGLPPAVA
                     NATNRLGVIFESGIGVKTFRSEGMRFEKSTLPAIVAATIGSVIGSMFVSTIPKDALEK
                     VIAVALLVVVMIMLLKPTPLTFSRPSRVLSTLIFLGVGFYGGFLQAGVGFFLIGALTF
                     SYGFDLVRTNFTKILIVFIYTVFALVIFILNGMIYWIPGLVLAAGNVVGAYFAARLSI
                     RKGSGFVKWILLIIVVANAIKYLFF"
     gene            complement(9833..10318)
                     /locus_tag="PKL93_04710"
     CDS             complement(9833..10318)
                     /locus_tag="PKL93_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNQ70596.1"
                     /translation="MSKKEEAAIPRGEELLKLDNQLCFALYSSSRGVTRLYRPLLSEF
                     GITYPQYLAMLVLWEKEPLTVKELGERLFLDSGTLTPLLKRMEKQGLLKRERSQGDER
                     QVLINLTEEGRKLKDKALAIPLKVADQVNISQSEFISLLTSLKKLMKKIDMGGSNNSF
                     V"
     gene            complement(10315..10794)
                     /locus_tag="PKL93_04715"
     CDS             complement(10315..10794)
                     /locus_tag="PKL93_04715"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNQ70597.1"
                     /translation="MSIYDFQLTTIDGWNKSMEDFKGKVLLLVNTASKCGFTPQYDGL
                     QQIFEEFKDRDFVVLGFPSNDFLFQEPGSDEDIKAFCSTNFNITFPLFSKIHVKGKNI
                     HPLYEFLTSGAGKKEYSGKVKWNFTKFLINREGEIIGRFEPKEEPESFRSAIEEAVM"
     gene            11124..11945
                     /locus_tag="PKL93_04720"
     CDS             11124..11945
                     /locus_tag="PKL93_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNQ70598.1"
                     /translation="MIKASSIAGKVETALVEANTIMDDKIKSSLYLYDGPFSSVLNEN
                     SILAEKLKLPLCQDTGFIEFFVFIGQEISLEEPISDTLNMAVRRAYSTNPYRYSVVKD
                     PLMHRENTGDNTPSVVHTFMVSGRELEIRFLVKGGGSENLSRLFMLNPTTSQEEFIEI
                     IVNSISEGGARGCPPLRVGIGIGGSSEKSMLLAKFALTRELDSRNPDVGYAFLEEKLL
                     NEINSLHIGYQGLGEGITAYSVSIETAPCHIAILPVSLAIDCYLCRKGVVTFEDR"
     gene            11932..12432
                     /locus_tag="PKL93_04725"
     CDS             11932..12432
                     /locus_tag="PKL93_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNQ70599.1"
                     /translation="MKIDDLSPGQELSFTGRLIVMRDAAQMRLKKLYEGGKTLPVELN
                     EAIVFYAGPAKLVKESCGAIGPTTSLRMDGFLEMLFEKGVLATIGKGERSVPAANLCK
                     KYRRVYLIAPSGAAASLAQRIESLRTIAYEDLGTEAIFEIDVRDFPLIVATDVNGSDL
                     FTLNRR"
     gene            12437..13660
                     /locus_tag="PKL93_04730"
     CDS             12437..13660
                     /locus_tag="PKL93_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNQ70600.1"
                     /translation="MNEKALKLHKDLRGKLEVRSKVRVDSMEALSLAYTPGVADVCRV
                     IESDKELAYDYTNKWNYVAVVSDGTAVLGLGDIGPEAGLPVMEGKAVLFKEFANVDAF
                     PLCIDIHSPEEIISFVRAIAPTFGGINLEDISSPKCFFIESELQKMLNIPVFHDDQHG
                     AAIVAVAALRNALKIVGKKIENLKIATVGVGAAGGATIKMLLAAGARNIVAVDRNGIL
                     NRNQPSTLLNEFHSEIVKEINPDNLSGNLEEAVKGADLFIGTSVAGLLSPEMVRKMAP
                     EPIIFALANPVPEIMPDLARSAGASIVATGRSDFPNQINNVLAFPGIFRGALDTRSRE
                     INESMKLAATDALASAVPEEKLSRDYILPRPFEPGIAKKVALAVGRASIDSGCSRLSG
                     EVNLEQLIERGFKKI"
     gene            13897..15126
                     /locus_tag="PKL93_04735"
     CDS             13897..15126
                     /locus_tag="PKL93_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNQ70601.1"
                     /translation="MTEKFSKIALRMKSNIIRELLKVTSKPGMISFGGGVPDPDTFPR
                     FEMAEISKEVLEKEYRFTLQYGSTEGDPILREEYISLLKRESGIEGLDVDNLLVTVGS
                     QSALDLVGKIFLDDDSLYLVSKPVYLGAASAFALRSNGYVYMDLREDGIDLDEVENRL
                     EEIALRGEINKVKFIYVISNFHNPGGVTISLEKRKRLVEIAEKYDVLIVEDDPYGDLR
                     YEGDPIDPIFKIGGQNRVLLLRTFSKILSPGLRLGIVIGNKTIIRKMVMAKQASDLCT
                     PSLTQRIAARYLQRHDLLAQLKPTIALYRDKRDLMLAELEKNFGDMKGFHWTKPDGGL
                     FIWFTLPESFNTGEMLEMGKAENVLFVPGEAFSLDNTCKNSMRLSFCLPPREDIIEGV
                     RRLKKVILEYGKEKNLL"
     gene            15139..16194
                     /gene="buk"
                     /locus_tag="PKL93_04740"
     CDS             15139..16194
                     /gene="buk"
                     /locus_tag="PKL93_04740"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNQ70602.1"
                     /translation="MKILAINPGSTSTKIAVFNDEEEIVRGSIQHFSSRNDRSQDIQV
                     RAEEILSFLKESSISFDFDAIACRGGILPPMESGTYLVDDKMVDFLLHRAAIDHPSNL
                     AAPIGLRLSEGKIPVFITDPISVDELCEEARLSGLPQLPRISRLHALNMKAAARQIAF
                     DLGRDVRNLNLVIAHLGGGISVGLQLRGKMVDVNNATDEGPFSPNRTGELPVGDVVEK
                     CFSGEYSERELLNRYLKYGGLLAYLGTDDLRKALEFAKEDDDAALVVDAMAYQIGKEI
                     GGMVAVAGGSIDAIVLTGGMAYNTEFVQKIKEYIGKFALVVIEPGENELLSLALGAKR
                     VLEGTEKARTFDPEVAM"
     gene            16191..17090
                     /locus_tag="PKL93_04745"
     CDS             16191..17090
                     /locus_tag="PKL93_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNQ70603.1"
                     /translation="MIPHLSQLIDRAKNNPKTVAIAAAEDSIVLAAAKEAAEHGIASF
                     ILFGQKKKIEQINNELGFSEGFTIVDCSTKLESIEKAVKAVSLKDAEILMKGNVKTGE
                     LLGIYLKDEFGLKTGRTMNLVTVFEIKRYHKLLLVTDAGMVIAPDLNQKADSIINSVS
                     VARALENKRPKVAVLAAIETVNPKMPATIEAAILSQMARRGQLGSVDVDGPFALDNAI
                     SREAAEHKGISSSVAGDADILIMPDIEAGNIFYKAMAFLSECQLASTIVGGKAPIILT
                     SRADSAETKLYSIALNVLLAGVI"
     gene            17090..18175
                     /gene="buk"
                     /locus_tag="PKL93_04750"
     CDS             17090..18175
                     /gene="buk"
                     /locus_tag="PKL93_04750"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNQ70604.1"
                     /translation="MSVVLVINPGSTSTKLALFRDSEMIGEHTIRHSPDELSKFPSLY
                     EQREFRKALIVRFLESVKHPLSEIDAIIGRGGMLRPLEGGTYVVNEEMIEDLRLAKYG
                     EHASNLGAILANELSLESGKGIPAYIADPVVVDEMEPVAKFSGHPDYTRRSIFHALNQ
                     KAVAREVASRYGKKYEEMNFIVVHMGGGISIGAHRRGRVVDVNNALNGDGPFSPERAG
                     TLPLTGLIKLCFSNEYTKDEIRKLIKGKGGLMAYTGTSDCQKVQQAILDGDRESELAY
                     KAMAYQIVKWVGRMAAALSGEVDAIIITGGIAYDSRFMVPWLTEKLSFIAPINVVPGG
                     NEELSLAVACLRVLEGSEKAKQYKGEP"
     gene            18172..18843
                     /locus_tag="PKL93_04755"
     CDS             18172..18843
                     /locus_tag="PKL93_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobQ"
                     /protein_id="HNQ70605.1"
                     /translation="MNFTKKILIFMGMFGSGKTEIALNVSRLLTKKGERVALADIDTV
                     SPYYRSRDMRDSFEQLGIKIIAPKGKLANADLPIIPPEIFGYVENPEFRVVLDVGGND
                     DGAVVLSSLSSRLPSDNCETFYVLNHFRPFNDTVENAASHFFRLQEKSRMKINYLVNN
                     TNIGLETTSETIDKGEEFVKQLSERVSVPVAFTVIMNGLNREPRLFEQLEIKKYMLNP
                     WEVEQ"
     gene            18840..19064
                     /locus_tag="PKL93_04760"
     CDS             18840..19064
                     /locus_tag="PKL93_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNQ70606.1"
                     /translation="MSKNFVKIDEERCKGCGLCINFCPKKVLSFSDKFNSKGYRPAQQ
                     HDLENCIGCGFCYMMCPDAAITVFKETVEV"
     gene            19075..20133
                     /locus_tag="PKL93_04765"
     CDS             19075..20133
                     /locus_tag="PKL93_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNQ70607.1"
                     /translation="MAERIMVKGNEAMAEAAVRAGCRLYFGYPITPQSEFTEYMARRM
                     PEVDGVFLQSESEVAAVNMVYGAACTGHRVMTSTSSPGFSLMQEGVSYIAGAKLPAVF
                     VNVVRGGPGLGDIQPAQSDYFQSTKGGGHGDYHLIVLAPGSIQEAVDLMETAFRLADN
                     YRIPVMMLADGMLGQMMEPVVFPEFVKLEGINDHSSWALRGADGREPHKVTSFDIDPV
                     KLEEMNTETHKVYEEIRSKEIRFETHDLEDADYILVGYGTMGRILGSVVKTARKQGIK
                     VGLFRPITLWPFPYDELKRAARGKKFCTVVEMSTGQMVEDVRLSVEGSLPVHFYGRTG
                     GMVPTPDEVFKELVRLIG"
     gene            20143..20895
                     /locus_tag="PKL93_04770"
     CDS             20143..20895
                     /locus_tag="PKL93_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ70608.1"
                     /translation="MEKATYKKPKSLAEKEFSYCPGCHHGIIHRLVAEVIDELDIQGK
                     TLMVAPVGCSVFAYEFFDIDGTVAPHGRAPAVATGMKRAMPEKMVFTYQGDGDLAAIG
                     TAEIIHAANRGEKITTIFVNNAIYGMTGGQMAPTTLLGMKTTTSPYGRKAETEGFPIH
                     VSELLKETAGIVYLERVSVSSPQEVIKAKAAIKKAFIAQMKGLGFSLIEVLSTCPTNW
                     GLDPIESIKWLNENMKKEYPKGVFIDKVGDIR"
     gene            20892..21431
                     /locus_tag="PKL93_04775"
     CDS             20892..21431
                     /locus_tag="PKL93_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ70609.1"
                     /translation="MSDHFLIAAGFGGQGVMLIGQILATAGMFDNKHTTWLPSYGPEM
                     RGGTANCTVVVSDEPIASPITNTPNELIIMNIPSLIKFEKEIQSDGLMVINTSVVDRD
                     TIRDDISIIKVDANAIAEKLGNLKVANMVVLGAYLKKTGAVTLEGVKKALEEKLTGSK
                     AALVNLNIRAIEEGMKSVS"
     gene            21567..22361
                     /gene="zupT"
                     /locus_tag="PKL93_04780"
     CDS             21567..22361
                     /gene="zupT"
                     /locus_tag="PKL93_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNQ70610.1"
                     /translation="MESENFWTALFLTSFAGLSTGIGSILAIFTKKTNTGFLSVSLGF
                     SAGIMIYVSFVEIFVKSGESLQKAMGDGISNWVNVIAFFGGIGVIALIDFLVPKAENP
                     HEMRDVDEMEASSKTLLRMGVFVALAIAIHNFPEGLATFLAALEEPSLGIPIAFAIAI
                     HNIPEGIAVAVPIYHATGSRKKAFLYSFLSGLAEPAGALIGFSLINILFNEIAFGVVF
                     AGVAGIMVYISLDELLPSAEKYGKHHLSISGLIAGMGLMAISLLLL"
     gene            22489..23589
                     /locus_tag="PKL93_04785"
     CDS             22489..23589
                     /locus_tag="PKL93_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ70611.1"
                     /translation="MLRLNWLNKVEVRSSTACDLLHGMFRLNNNDRFLELFASPQFKG
                     KFEPYRNIQTWVTETRELLTSDIVEKLKEFFDWETFLGMCLLPDITHSNLESASHLIE
                     FVQEMPERVLLIHFIYSGYGPRMGTVDMDTFDKIMKNDRDMLLFVSNDMSFSTERKAI
                     LFEMLSHPERTKEDLLYLLEWFYENVFSTIEPRIAKIHSKAVNEIEREISATGEKFLR
                     TIIKNIDFTRSEVLERTVICPSYFSEFLISNASIPFVKEDMYTIGFRFKEVMAVPKEA
                     LEITAETFDALAHEKRLAIIRYLSTGRSSGNELARALNLSNYEVGEHIGILREAGMVL
                     AKKANQVTYFTLDKAIVRQEISRALEDLLESS"
     gene            complement(23592..24071)
                     /locus_tag="PKL93_04790"
     CDS             complement(23592..24071)
                     /locus_tag="PKL93_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70612.1"
                     /translation="MSDEKRIQLKNRGIQLHSSFQNERREAAEEKADIRDALELYDFS
                     LKTRIVSDYSVESLVSLSSIKKMCDNLEKKLDRQKKSFRPHSVVMKGESVTPVEIILK
                     ETPLKQWYTTGKTLGSLASIGAKVALQLLKNQKDEKSFRKLRLLEIEIMHRKLKEDH"
     gene            complement(24068..25027)
                     /locus_tag="PKL93_04795"
     CDS             complement(24068..25027)
                     /locus_tag="PKL93_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70613.1"
                     /translation="MLFLLIVVAILLGYVAYRLILREGGIFLGPYAIKFRKEPGPEDY
                     LRRLKELQQRNQDFESRLVLGAATSKFPENLEFFKLAMDKVFSDLRDAKSEKEVEEVF
                     LRGERLLKEFGAASSTNSIGVVTEYSKRLVQAQQEFYSLRKERDMELERKRYERNEEI
                     LKELESVLEGIRASNDEMAIRDEMNNAARLETGLDLSILDEGQNERYREVKNGFYRMA
                     EEKVESLRSARYARYNRKAIERLKKLIDEFSENEKELSKSGSSLPVILKERIGSLNTS
                     YFDGPTMQYFNYVYGYIFSLIDEDLKFEVTRIMTETEKDALEV"
     gene            complement(25169..26218)
                     /locus_tag="PKL93_04800"
     CDS             complement(25169..26218)
                     /locus_tag="PKL93_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNQ70614.1"
                     /translation="MSRRRIEGLTSSDERVVWIDVARGFLMALVVMYHTLPPQIAANL
                     INPAACTFFFLSGLISRDIPLKDSISKRFKQIMIPYYTMALANTAIWLIARAIVPRQE
                     MNFTLGSVIANILTVRTAVGIIPLNIIPLWFLPTVFVMEIYYSLLRRLRILIVGITIG
                     FVSMYFFYGSLPFKLDVALAVLPYFALGKFVRSRDLFSKRLSFLFTALACSLYIITSA
                     FCNEVYLMEDYFGTLPFLYVIAALIGIIAVSGLSQILQRSKAVRNFLSLFGRRTLFVL
                     GYHIAAGFLVYPLFDLIGDPIEIMERFWFIYWIINMGVLYLMIRYIPEPVITALSGSF
                     LAKRQRNPEKLTHGK"
     gene            26564..28678
                     /locus_tag="PKL93_04805"
     CDS             26564..28678
                     /locus_tag="PKL93_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70615.1"
                     /translation="MKKIILMTVLVISCITFAQQFDELFNNLLLKSEDFVSFSGAGFD
                     ARFVPTYAATDSEIRFSDGQNLLGILVVRSTRVMDIEINNVVIINSEDDSRMAFKIYK
                     VEDLTSPVELPFSLPSGGVLVLTSETPLQDYFMDIRIQQILLPWRVKTSEPDRVVEMA
                     PVTLVETASEVDVEGNDNYDSLTVSISDLNDRLDFLQDNVEGLNLQFRRLNTLFLDTQ
                     VALAEYKAAVDEGAFDTEEFAMTIENRLTALEESVVDNPITRIEFEELSESLDRLYSS
                     LAEWSDEISKIETLEASQTGLQLSIENLENTVRSLREITPQQSDSEDIVSINDKIGEI
                     TTSLSAAENELQEMKSFIGLQSEEISTVRNELGDALDSLTARLNFVELSAKTSSESIG
                     TNNELLAEITAEVDALSDYVSEQALSQMEKFSSLSEAFSDIEKRVIAAETSLSDFEEI
                     KARLSASESATRILSDRISSLEAFYSSAEDIIPILEEKVGNLETSVFGTGKINMDELE
                     TEILQLREIADSLSRGFVKFNSELITLRESIPPEGVSIETFENSINELSDAIININAD
                     LSEIRSSVAELELSLGVLEDSLVGVSVGLDSMKGNFDGSVAAIDSNLKAIQKIENDLL
                     LTQEDLAKTRDQLSTLTKELEKTLVTREEIITITEEAVKQSREATAQEISSLRRTNNI
                     WLTVAVISSLAAIVLGVVNILP"
     gene            28698..29723
                     /locus_tag="PKL93_04810"
     CDS             28698..29723
                     /locus_tag="PKL93_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ70616.1"
                     /translation="MKLKREILIIATSCVLLCPTNFSAYSLSEIGELAELLKQLFLAS
                     RTARDFSLESLKVDTEEFPAVDFVVKEDVVYSEVDGEILTMDVYYPARTTAPRPAILY
                     VHGGAWITGDKRSGPGVVVTGELIRAGFIVFSIDYRLAPKWKFPSQVIDVKTAIRFVR
                     ENSTELLIDPEAIGGFGTSAGAHLVTLAALTANTGMFSEDKYSGQDENLFCVADLYGP
                     TDLEALFEGIEREVAELIFGNEEGILRKASPINYVNKDSPPFLIVQGDSDLVVPVDQS
                     KRFFEELTAAGCYSKLVVVENAGHGLVPDGGEIKPSLIDVAKIVVGFFQRQMIIQQSR
                     INLLLRF"
     gene            complement(29683..31341)
                     /gene="hcp"
                     /locus_tag="PKL93_04815"
                     /gene_synonym="priS"
     CDS             complement(29683..31341)
                     /gene="hcp"
                     /locus_tag="PKL93_04815"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNQ70617.1"
                     /translation="MFCFQCSESMKGTGCTVQGICGKEPEVADLQDLLIWILKGISLW
                     GVKAQEMGVKDSETGLYVAEGLFTTITNVDFDPESLGKKIDRALNVRRRIERIFKEAY
                     RKEKGKEFSEPMPEACTWESSRGVESYKAKGAQVGVLDTQDEDIRSLRELLTYGLKGI
                     AAYTDHAYILKHSDNSILDFLQEALASCLNDSLTAEDYVALVLKAGEYAVKAMALLDE
                     ANTGSYGNPEITSVYTGTYEGPGILVSGHDLLDLEELLKQTEGKGINIYTHGEMLPAN
                     GYPGLKKFSHLRGNFGTSWYNQQKEFEEFKGPILMTTNCIQRPRESYKDKIFTTGLVG
                     WPEVSHIPNRVDGKEKDFTPIIEKALEIGDIGARPGKKIVVGLAHDQLSKVSDKIIDA
                     VKTGAIKKFVVMGGCDGHAKKRQYYTDLAERLPKDMVILTAGCAKYRYNMLDLGDIGG
                     IPRVVDAGQCNDSYSLVVTALKLKEAFGLEDINDLPIEYDIAWYEQKAVAVLLALLYM
                     GVKGIRLGPVLPAFISPNVLKVLVENFDIKPIRTVEEDLSEIVG"
     gene            complement(31424..32203)
                     /locus_tag="PKL93_04820"
     CDS             complement(31424..32203)
                     /locus_tag="PKL93_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70618.1"
                     /translation="MRKVFVIALSFFVLLSSLFAVFMEEDLSALQFGFSGKVHTVQIE
                     IAKLGAELLISDPETLTFNEKGFLISRETEEVKIEYFYDDSGRLRRKDFINRDGTVFQ
                     RVEVLYEDDHYDAVAFDDSGIELERTRYWINSEERTLIFSVETETGVSTNTLFFDENG
                     RKSERYSLIRENVSELDMEVNIVVESTFTYDSNGHLVGEKTVVRVRREGREQASEFRS
                     RIDILLTDEQGNPVRELRRIEFLDSSSAPEEFIYSRVYTYY"
     gene            32367..32876
                     /locus_tag="PKL93_04825"
     CDS             32367..32876
                     /locus_tag="PKL93_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNQ70619.1"
                     /translation="MTFKELSERSGIRYETVRNYVKVLIEEGLIDEVSEEVVEIVKKM
                     PDYTSQGLTVVEAAHRAVSGEQKDSRTSTETLKLKERIACLEEENKLLKAELDKERAA
                     TKQLEEKIQSIEGGQTSSSGIAVYREEAKTAAEALKTAIKSAGSGFVQFLQWLFGAEA
                     EKVEEKSEK"
     gene            complement(32958..34208)
                     /locus_tag="PKL93_04830"
     CDS             complement(32958..34208)
                     /locus_tag="PKL93_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70620.1"
                     /translation="MKKLLLIALTVIFAVGLFGQLGLRTLTPEPTETADASETGEIVP
                     DSEELNTLSLRTIDPLLVSFREGWTSPDDPSWKVEMIGSTEIMIPQDFSIFQEKTGLN
                     YDVQIFDKEGLLFGRLFLYQLESYARSELISAVVGSLYGEGATTESSYEETVELHDGR
                     IAYLVSLKIRPEVDYPFVVVYSPSEEADTTEPGAVTMCVFEPLHYSGSELDKAKEIIA
                     GIVGSLISAEEEPEEEEEVIALPSEKEEHTDIFVRMVESLLSEEDLDIYIDDWIEVEG
                     DYFAFMMPPEFSVAFYLADYFEVADLGFQGTVVGKIFVGESEESASTSDVLNQYVYQY
                     LGSFGDYELVDSYTGYVDRESVVNVYALDFSGQLCWMALYSESHDAEFFGPGEYFVLL
                     GLADYEEAVMWAEWYTGILATLDF"
     gene            complement(34316..34942)
                     /locus_tag="PKL93_04835"
     CDS             complement(34316..34942)
                     /locus_tag="PKL93_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ70621.1"
                     /translation="MSGLSFDDLYQKASEVLEIYNDACRTIRDIQIETGLRCLPSCRE
                     CCKTSGDSIQVTITEFLPLSLRLWNEGRADDLLRRLEDVPDSDRCILFEGSGEIRNEG
                     GCTEYAFRPLICRLFGFSGVIDRLGRVAPVICKYVKTGYPLSVERFTEKFSSGLEIPV
                     FSDYLKRVQGIDPHMGSRTYSINSALRKALEYVGMRFYYSSKGFDRTA"
     gene            complement(35432..36754)
                     /gene="xylA"
                     /locus_tag="PKL93_04840"
     CDS             complement(35432..36754)
                     /gene="xylA"
                     /locus_tag="PKL93_04840"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNQ70622.1"
                     /translation="MSVFDKIEKIQYKGPDNKDPMAFSFYNPEEEFMGKTMKEYLRFA
                     VAFWHTFQGDGLDMFGKPTMRRSWDSIKDPIDLAKLKVDAVFELCEKLSIDFFCFHDR
                     DIAPEGETLRETNKNLDEIVRHISDHLRTSNVGILWGTANLFSSPRFMHGAATSCDAD
                     VFAYAAAQVKKAIEVTKELDGKNYVFWGGREGYETLLNTDMGLELENLARFFHMAADY
                     AKEIGFSGQFLIEPKPMEPTKHQYDFDVANALAFLRKFGLEENFKFNVEANHATLAGH
                     TFQHELRFARINDLLGSVDANQGDPLLGWDTDQFPTDLYSTTLGMYEIIKNGGLNPGG
                     LNFDAKVRRGSYDERDLFIGHIAGMDTFALGLKAAAKLIDEGTLESLLKDRYRSYQSG
                     VGKEITEGIADFRSLEEYIIDKKNPLPEPSNQEYLERLVNWAIISAGK"
     gene            complement(36769..38244)
                     /gene="xylB"
                     /locus_tag="PKL93_04845"
     CDS             complement(36769..38244)
                     /gene="xylB"
                     /locus_tag="PKL93_04845"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNQ70623.1"
                     /translation="MSAFFGIDVGTNSVKGISVDESGRILTSSSNPIPMETPKPGWSQ
                     QHPEIWWDAVCIALRELVAKGGGPPSAISVSGQMHSLVAVDAEGKVVYPAILWCDQRT
                     ESQCAKVTDLLGGEEKVIGSFGNPVLTGFTLPKILWLAESEPERFKKIKKWMLPKDYI
                     VMKLTGRVVTDFSDASGTSMLDIEGEFDERIPEIIGIDPRSYPELVGSGTIVGTVSSP
                     SLSELEGIPVVNGGADNASSAYGCGVEKIGDAMISIGTSGTVVALTKRGIPDRSGGIH
                     LFRHVTGRDFYHMAVILSATNSLNWFKDRFGNDLSFESLEEMVKQTPAGSDGVVFLPY
                     LNGERTPHRDPSARGTLFGFSSFHSRGDVFRSIYEGVGFALREGAELIESLGSEMNNV
                     RIVGGGSRSETWCQIVADNLGKTIWLPEVDEGAAYGSARLAAEALGIGTSSWIRMKKE
                     FRPNGDVRAVYDNVFSIYKELYPKIRDSYKKISKLQESITK"
     gene            complement(38257..>39199)
                     /locus_tag="PKL93_04850"
     CDS             complement(38257..>39199)
                     /locus_tag="PKL93_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ70624.1"
                     /translation="NRKAFMSVVIDIGVEETIIGKGFFDGSVSVVSRFRTPRDFDQFM
                     DLLTKKTSLISRNIPKSRFLGYSLSVPGIVDVENSRIVYVPHLGWKDLVLRERLSRRY
                     PVFLDNEANLSLIAEKWNNPDVVSVRDIVFVYVSEGIGCGVMFDGQIYRGRDYSAGEF
                     GHMTVQTDGKKCYCGNFGCWETIASTEAIVNLATELGLELKGTSNNEKYLNCLRSTDA
                     SYEPLLREIERSLGVGIVNIVNSLDPEVVVLGGVASILPEVSLRNLLDFVNSRVLYAT
                     GQNVKVIRSMLHEKGMASSKMIGAALHIIDRKTVDLV"
     assembly_gap    39200..39299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39300..39587)
                     /locus_tag="PKL93_04855"
     CDS             complement(<39300..39587)
                     /locus_tag="PKL93_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNQ70625.1"
                     /translation="MVGKKLDSENIGRSNRKLILQLLRKNPATTRRDLAVASGLNPST
                     VTKIIKDFLSMGLCEEVRAEETGRIGRKAIVLRLNRKAFMSVVIDIGVEETI"
     gene            40086..41186
                     /locus_tag="PKL93_04860"
     CDS             40086..41186
                     /locus_tag="PKL93_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ70626.1"
                     /translation="MLLERIKYIDWPAYDFLLCLMRVNCNEMIIEAYKEQDFKDQFVP
                     DPELLEWAKETKKILPDDIKELLERFFDCETYFGAGTFSLVQERNLREPEEFIKALKS
                     TSEKSILGRFLRTGFGTDVKSRDSSQFEELVERLTVDEKEMLLFITESTIFSSFQKAN
                     LLGFLENPAETKQDLIYLFEWFLENVFNRMRDEVRRSNEKHLKNLEEYIRKGGTSYIE
                     KLNLLSFFDILETVQTIELSVSPFLGLDQAGLMVMKDRYLFILGYDRIEVPFREKDEK
                     LQCIDVFSALAHKERLAILRELSREPHRPVSLAKKLRISAGDLSNHLEKLKKGRLLES
                     VYNGEYVEYTVNAKELKRLVSESLDKLLEEQN"
     gene            complement(41264..42052)
                     /locus_tag="PKL93_04865"
     CDS             complement(41264..42052)
                     /locus_tag="PKL93_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ70627.1"
                     /translation="MERSIAEQYVRIVDKKIFYLETVGDDEIPVVLVHGNFASSKWFE
                     PALKILPSGLKGYAIDLPNFGRSDRLEEVSIDNYANYVINFILEMELDGVVLVGHSLG
                     GAVAQSAVIKRPDLIDRVILVDPAPPDGLKTPEEVYPYLEMYRGNRELLKKALIGVMP
                     TREVDEFLEQLVDEALMMDGRCFVENARALEKYDFTEALSEIEVPHLVIVGKLDNIVT
                     EEMARRFEKILQDVEIRVLPDYGHSVILEDTELFVKILEDFIER"
     gene            complement(42259..42963)
                     /locus_tag="PKL93_04870"
     CDS             complement(42259..42963)
                     /locus_tag="PKL93_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70628.1"
                     /translation="MLEVKNLFVSYGRIQVITDFSFNVGAEAVGLFGPNGAGKTTLIN
                     SIIGLVKPRSGEILFEGESLLPYETHSIVRKGISVVPQDGELFPIMTVLENLKIGASY
                     IKEARDKVNERLEFVFEIFPVLKERAYQLAGTMSGGQQRMLAVGRSLMAVPRLLILDE
                     PSVGLQPTLVTELFEKLSMIKSEGVSMILSEQNVRQGLKVIDRGYVIENGTLAIESTA
                     DDLMHNEHVKRSYLGL"
     gene            complement(42963..43679)
                     /locus_tag="PKL93_04875"
     CDS             complement(42963..43679)
                     /locus_tag="PKL93_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70629.1"
                     /translation="MSILKLDKVTKRFGGLVAVDNVSMEIQQGEILGLIGPNGAGKTT
                     LTNLISGVHMVSEGKIIFDGIDITFLPAHKRCNLGIARTFQIARPLKNLTVVENIMVG
                     GLFGNGEKLKESKKSALMLCETIGLEEPDQTLDKITVLDIKKIEIARAMATKPKILFL
                     DEVMSGLNMDETREMIDTVRKIRGTGVTICIIEHVMSVIREITERVIVLDRGTILAEG
                     PYSEVSQQHNVITAYLGEDA"
     gene            complement(43681..44619)
                     /locus_tag="PKL93_04880"
     CDS             complement(43681..44619)
                     /locus_tag="PKL93_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ70630.1"
                     /translation="MKITKSLVIIMLLIAAALLPVVTEAYFISVAITILTFMTLSISW
                     DMMLRTGQLSFGTAGFFGLGGYTGIIAVADFGVDPLVGIILGGLLAGGVSLLLGMAIL
                     RLRGLYFAITTMALASVFMVIIRNLNKLTGGPSGKIVYTTLFNYDSVKVYWLMLGITV
                     GTIVVSEVFQRTRVHFAIDSIRADETVAKSHGVNIYKYLIFVFVVTSIIQGVIGSTYA
                     LQYGFVYPESSFSAHYLLLPIAMALVGGIYSTIGPVLGAIILGLSSEYLKLIMPYGHL
                     IIYGVIIVVVVLFLPNGVYGAIKDVTGFRKRRLEER"
     gene            complement(44616..45545)
                     /locus_tag="PKL93_04885"
     CDS             complement(44616..45545)
                     /locus_tag="PKL93_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ70631.1"
                     /translation="MESISFMRKHRNLFLLIAVIVVVAIWKPHAAIYGLQRGSLYSLV
                     GLPLSLTLGVVGIMNLAHGDFLSLGIYFVYLFATNLGMDPLVSIIPVFFLLFGLGVVI
                     YKLTITRVMKSGHLNQLLLTFGISMILLEAMNVIWTSRPRSVYVEYSSSSTTIAGVRF
                     GTYELLYVVFAFAVLIALQLFLKKTRLGQATFAVGQNPQGAKIVGINTGFVYLFVFGI
                     SIAILGIAAGVMVPRFAIFPSVGSAFTMKSFCLVAMAGLGNLTGILVSGIVLGVGEAI
                     VQSIPGYAGWSDIVFFGVLIAVIMIRSLRRQSL"
     gene            complement(45615..46850)
                     /locus_tag="PKL93_04890"
     CDS             complement(45615..46850)
                     /locus_tag="PKL93_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70632.1"
                     /translation="MKRVLLVLLVVAVTAFVAFAATPIKIGAVVPLGDITGDQSAKAM
                     KLAIDEINAAGGLLGRPLELIVVDSELKPEKGAAAIEKLATVDKVDFFVGGMASSVHL
                     AQIPVMKRYQKITIWSGAASYLCENAVGPDADWYFHLHPWDYQQGASYGLGWTELAEA
                     YPDIEISKIFLAYEEGGFGSASHESYLVLQSQANQGEGSWAAVMGDYMGVSFKSAALG
                     GGDYRAMLQQAKAYDPDLFVWAGYDADALPMLQQAKEIGFAPKFFVGAPPGWPAHFGD
                     SPLAEGVTLYGMWAPTINKVSPTAEHFWNAYIEKYNEEPATYFAPLGYTNIYFLAEGI
                     KAAGTLDKAELIKALEEVEYASPIGEVLKMSPSNVIKHQGFTGQKILQWQGGEQQVIW
                     PLNLKTAEPIYPFPSWEGR"
     gene            complement(46948..48147)
                     /locus_tag="PKL93_04895"
     CDS             complement(46948..48147)
                     /locus_tag="PKL93_04895"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNQ70633.1"
                     /translation="MKKVFIVGAKRTAIGTFGGTLKDVPAVDLGVTASKAAIEQSKVD
                     PKNIQETIMGCILTANQGMGPGRQVSIGAGVPIESVGYSVNMLCGSGMKSTMIGAQDI
                     LTGEAELVLTGGMENMSIAPYMLDKARFGYRMGSGTVVDHMIRDGLTDVFNDYHMGVT
                     AENLAEKYGISRLEQDEFALESQKRAKEAIATGRFKDEIVPFVIKGRKGDVVFDTDEH
                     PRDTSMEILGKLRPAFKKDGSVTAGNSSGINDGASATVLASEEAVEKYGLKPIAEIVS
                     FAQAGVDPAYMGFGPVPAVEKALKKVSMNIKDMELIELNEAFASQSLSVLKGLMEIYG
                     VSKEWLLERTNVNGGAIALGHPIGASGNRIIVTLLYEMEKRNLEFGLASLCIGGGMGT
                     AVVVKRV"
     gene            complement(48159..49457)
                     /locus_tag="PKL93_04900"
     CDS             complement(48159..49457)
                     /locus_tag="PKL93_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ70634.1"
                     /translation="MNWNDEYRKKKISIDEGLRNIKSGTKIVTSMAAMEAQGLLKNLH
                     RVEDVENVAVATCLNIGEYPFFVNKEYEGRFLNESWFHSVGARKAVSSGLRTVTYIPN
                     NLHTAGIERIMANKPDIFWGVASPMDRNGYMTVSLSTVYERDMVENAKMVILEVNEKA
                     PKTHGDTHVHISEVDYVIENSFDIPELPDTEPSEIEMRIAGHISSLVVDGATLQIGIG
                     GIPNAVARLMEDKHDLGIHTEMFTESMIHLFEKGAITNRKKSLWPGKFIATFALGTKE
                     MYSFIEDNPGVLLLRGSYVNDPYVVAQNENMVSINTAISVDLTGQVCSEAIGTRHYSG
                     TGGQLDTHRGASMAKNGKGIIALRSTAKKGTVSTIVPLLPLGSPVTVPRQDTDYVVTE
                     FGIARLKGLNVFQRVEALLNVSHPDFRSELRKQARDIGLI"
     gene            complement(49454..50632)
                     /locus_tag="PKL93_04905"
     CDS             complement(49454..50632)
                     /locus_tag="PKL93_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ70635.1"
                     /translation="MKVSRSERTRLQIKEAAEELFSRLGFEMTSVANICRTCGLSNGA
                     FYRHYTGKEQVFKEIVQDIKTRFESASASVMGNTIEEKLYNLYRKVFDILWESRRKFI
                     AFHEAEYRFPDVENSVDKTYKDALLSTVINDRSPLSMPELWFLIGSSRFAAIYWIIYN
                     DTRVPDSTVRSLVDFALNGFVKSDELDRRSLDFTLDEVRADGEGKAKEVILMAAEKLM
                     GEKGYFGTSIYDITSKAGYGQGTFYLHFESKGKLLQELVYQANRNLRRTMRDAVSGVE
                     GRLNKEIRSFKAFLLFMDVHKELYNIVRESEFVQPETGRFYYERLLTSYIRALEESRL
                     KDEIRSIDSRDLGVFLMGIGHFMGLDLLFKLKKDHEKWNEYLLELASLMAKGYGGAFS
                     "
     gene            complement(50629..51375)
                     /gene="fabG"
                     /locus_tag="PKL93_04910"
     CDS             complement(50629..51375)
                     /gene="fabG"
                     /locus_tag="PKL93_04910"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNQ70636.1"
                     /translation="MRLEGKVVIITGGASGLGKAAVEKFAREGAIVYACDMDVEGLDN
                     LKKEFSELPGKVIPKRLNVTDRPAITELVGEIKSEFGRIDGLINNAGVTRDALIQRMS
                     EEDWDLVINVNLKGVFNMTQAVAPVMIDQGYGSIVNTSSIVGVYGNIGQSNYSASKGG
                     VIAMTKTWAKELTRKGAKIRVNAVAPGFIKTPMTEKMPEKILVALEEKIPLKRMGLPE
                     EIANVYFFLISDESSYLTGQVIGVDGGLVI"
     gene            complement(51387..52409)
                     /locus_tag="PKL93_04915"
     CDS             complement(51387..52409)
                     /locus_tag="PKL93_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNQ70637.1"
                     /translation="MSSVPIIGIAGIGTYIPETFMTPEEIADSTGIPQEVIELKFGVK
                     RRLVPGPGETTSNLGIKAARKAIENAGIDPKDIDLLIWNGAQHKDYPCWLAGLKVADE
                     IGAVNAWSFDMEAMCGSMMAGIDVAKSLMIARDDVNTVLLVSGYRNVDLMDLSEPSTS
                     FMLDVGASGAAVVLKKGHNENVVLSSAFKGDGSFSELCVVPVGGTKKWPMTPEDTQNY
                     HFSVQGDTAEFKKRLGETTMPNFYWVIRESLRKSGFSESDIDYLAILHFKRSAHFAVL
                     EELGLKENQSTYLDYYGHLGQNDQILSIELGLREGKIKDGDVVVMVGAGLGFVWAATT
                     VKWGKN"
     gene            complement(52582..53391)
                     /locus_tag="PKL93_04920"
     CDS             complement(52582..53391)
                     /locus_tag="PKL93_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ70638.1"
                     /translation="MALLRTSDGTQIYYEVHGDQSNPPVIILNGIMMSVPSWAGFVPH
                     FSRHFRLILMDFRDQGRSERKDSQYHISIHVDDLTDLIDALEISSANVVGLSYGGQVA
                     EMLAIKRPDRVNSLVLANTPARISPYLAELGEAWKEAAELKDGERFFKLAIPFIYSDF
                     FYNRNLQWLKERQKMFKSTLNREWFEGFIRLISSNPDFNVLQELDKIQCPTLLLGADR
                     DIITPIEEMKAIHERVRNSEFLVIKDAGHGAFLEKPGEFMTAIIGFILKNC"
     gene            53517..54530
                     /locus_tag="PKL93_04925"
     CDS             53517..54530
                     /locus_tag="PKL93_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ70639.1"
                     /translation="MIDLSKFLEDMLEQSEAVRSIISASDVVFEKAKSLNFERVLFTG
                     MGASLHASEVAASFLRYSGIDAESFEMSEIITYSSQELLKRYSTIFLISQSGESAELI
                     RFIEENKALLPSMTLVTNNEKSSAAKAFPDERVFPLFAGNERSMGATKTFVNSIVLTL
                     MISAFKTARRLSFSDLPTRMDEALSHDLSWLAALLSEKRERILVARGFGIGVGKMARL
                     MFAEVAKLSLIFYSGASFRHGPVDLLADKPVVITMNPKGETYDLMQELHNDIYDKCEL
                     ITLTNSREGNVKSIYVSDGLEEVLAFIPMMNLLQKTAEEIARNRGFDPGSGVFGTKVT
                     TKE"
     gene            complement(54732..55403)
                     /locus_tag="PKL93_04930"
     CDS             complement(54732..55403)
                     /locus_tag="PKL93_04930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ70640.1"
                     /translation="MSHLKQHYDRGKLEQEFDNLPEASPIADIDPFKGDIIAMVGALA
                     LDYLDLDPGDTLLDIGTGRGRWAVAASPYCRKVIGIDISRRMLEDARSNIASAGVENV
                     EFFRGSFENPCEEVNLSRMNINKVVAIYSLHHLTDPMKMEAISKLTQILKRPGRIVVG
                     DIMWFDDPGKHRDAWDEVYFDDNELDHPASISFMKEVFQHSGANEIEVVQIHPLVGLI
                     STTFL"
     gene            complement(55474..56448)
                     /locus_tag="PKL93_04935"
     CDS             complement(55474..56448)
                     /locus_tag="PKL93_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ70641.1"
                     /translation="MTKIAILGIAHMHGYSYVRALKRMNDIEIVGLYDEDRSRMNKAC
                     EDLGIKPCEKPEELVARSDGVIVTSENSTHREFVEIAAGAGKHILCEKPIATTVEDAE
                     AMIKICRENNVKLQIAFPVRYSASVRKAREIIAGGLLGEVVSLVGTNHGRMPGGWFID
                     PVLGGGGAVMDHTVHVVDIVRWLLDCEFTQVYATCGTLIYDIPVEDCGLEMFRLSTGQ
                     YMTLDCSWSRPKAHPYWGDVTLHIVGTEGTLFLDVFNSKIEVYSNEKGSRWEAYGDNF
                     DCLLIEDFVEVLRSGKDPFTRGEDGLEALKVVDAAYKSFREGQEIQIC"
     gene            complement(56450..>56959)
                     /locus_tag="PKL93_04940"
     CDS             complement(56450..>56959)
                     /locus_tag="PKL93_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ70642.1"
                     /translation="SGGVFVDLSIHDFDFLRKLLGPVKTIEARSVTLSSERKKNSFDH
                     GMAILRFESGALAHIEGSWAEPAGMPVNFGTFYEFVGTKGMITNSYERETTLRLQTSI
                     DGKPRYLQENPAYFDPYTEELKSFILSVEKDREVPVNGQEAIESLRVALAANMSAKLH
                     RPVELSEVF"
BASE COUNT        15799 a        13344 c        12178 g        15538 t
ORIGIN      
        1 tgatacttcc atttgtggat aacagttcaa gaactccgaa atctggaagg atcgcatcac
       61 agacaatctt cgcgtttgag gtttggaaaa tattcctatc accgagaagt gtgctctcag
      121 tgactgtgat tgctccgttc gtggaatttg ccgacacact gatagttccc tttttgattg
      181 ttataggagc attgcttgtc tctatgatga aggttcctga tattccattt gcttcaaaag
      241 caccgttggt ggatttgagt tccatgaccc cggcacattc ttcgacaaaa atgggcgaat
      301 tagaggtttc aagcgttatg cttcccgaga cggagataag ctccaaacct ccattgctgc
      361 ttctgcctct tattgaggca ttgagtgatc tgaaaaccat tgcgccgttg ctggtattca
      421 ctgcgatatc ttccaccgtt tccgacggaa cactcagata aattgttgtt gcctgccccc
      481 tgaattcgtt tgtttgagat agtttgggaa caattacagt gagttttcca tccattgcat
      541 tgatgaccgg tctagtcttt tcaagatact cttctttgag attttcgtct gcaactctaa
      601 ttgaccgctc taccttgatt tcaacataat ctctattcca gcccactact tcgacctttc
      661 cgttctggct gtcgatttcc aatgagttta ttttctcggt gactgtctct gagaagtctt
      721 cgttgaactt cactccattg aatgaccagc cagaaccaaa gctgaatccc agaatttcag
      781 gacggattat cactgctgca ccaatcaaaa ggacccctat tcctagcagt agtcccgcaa
      841 gcgccagacc tctctttctg ttagttttcc tcataacatc actcccattc gtacatcgcg
      901 gccagttagc tctcgacaat atatcactaa attaaagagt tttcaagtta tgcgttgtta
      961 ccgagaatcg cgtttgaaaa gcaaccctct ctttggtgac agactccatt tttaacattc
     1021 cagtaatatc gcgcttcgtt ttagagtaag aacccttcct ttcttacgga agggtcaaat
     1081 gcgagttgca agattttagt ggaatatggt catccctcag ggatatgccc aactgacggc
     1141 tacttgttat atgattgcaa taaggaaacc caacacaccg ttggaccata cagctatgcc
     1201 ctatcacaat tcgctgtttt ttcgctgtag aacattcaag gaggagttaa atgactgaga
     1261 agaagtactc gattgaagaa cttatggaga atgagaccat tgcatcattc gcggcatctc
     1321 ccgattacaa aagaattctt tactcgtcag acaaaacggg caactacaat atctttgaac
     1381 tagataagga gaagggagaa cacaggcaga taacatcact cagtgaaaac gcattggtat
     1441 cccacgtcta cgaagatgga agcttcattt ttgcaatgga caagggaggc gatgaactac
     1501 atcatctcta tctcggaact gaagaggaaa tcaaggatct gactcctttc gaaaagacca
     1561 agtcaggatt tcacgtctcc gttggcaata gggtttacta ctactcgaac aaggtcgata
     1621 gaagtagatt cgatctttat cggtttgata aggccgatct agcaagtgag ctggtcttcg
     1681 agaataaaga cttgtatgaa ctgggtccaa tttcgcatga tgaaaggttt ctcgcccttc
     1741 aaaagcccga tactgcaaac aattctaatg tctatctaca tgacttgctt agcggggaaa
     1801 ctacacttct gtccccccat gaaggagaag caaatttcaa acctctcttc ttctcaagag
     1861 actcgatgaa gctttactat ctatcggaca ttgaaggaga attcatgacg ctctgggtaa
     1921 cggatgttgg aagcaaggaa catgaagaga tccttgcctt cagttgggac ataatcgcgg
     1981 ggaggatatc ggaaaacggg gaaagcgccg tgcttatcgt caatagagac ggtttttccg
     2041 aactccatat aatcgacaca aaagacagtt cccccaagat gtctcccatt gagacgggcg
     2101 gcgttgttta cgatcaatgc ttcagcaagg acagccgctt cctgtactat ctttgtgact
     2161 cggcgacatc atctcccaat atctttgcga tcgatctcga gacgaaggag acagagcagc
     2221 tcactgattc catgagcgat gaagtgaaca gcaatgacct ctctgaagca aaggtactcc
     2281 gctttatttc atacgacggt ctccaagtgc ctggtatttt ttatccgcct agaaatgttc
     2341 cgccaaacga gaaagcccct gctattgtat gggttcacgg aggaccggga ggtcagtctc
     2401 tgccgagata cagtcctgag atccagttca ttgcaaatca tggatacgct atctatgcgg
     2461 tcaataacag aggaagttcc ggctacggaa agagcttctt caaagccgca gatcacaagc
     2521 acggggaagc agatctcgat gattgtgtag ccgccgccaa gtatttggcc accctcgact
     2581 tcatagacga agaaagaatc ggaataaacg gtggaagtta cggtggattc atggttctgg
     2641 cggcacttgc tttcaggccg aaggagttca aggttggtac ggattttttc ggtgtctcga
     2701 actgggtgag aactctaaaa gaagtgcctg cctggtggaa agctatcaaa gacctcttat
     2761 acacgaagat tggaaatccc tttgaagagg aggaatacct caagagcatt tcccctctct
     2821 ttcacgccga aaagattgaa agaccactcc tggttcttca gggtgtaaat gatccaagag
     2881 tattgaaggt ggaatcagac gagatcgttg agagtcttaa gagaaatgat gttcctgttg
     2941 aatatgtagt gttcgaagat gaaggacatg gcttcaagaa gaaagcaaat aggataagag
     3001 gtgcaaatac gatgctcacc tttcttgaca agtatttgcg ctaggaatat tcagttaggt
     3061 attagtgagg gagaaagctg aatttacctt tcgaagacga gaaccaagta tttcgtgacg
     3121 attaggagga gcgttgtaga gagtttcgta tgccttttga caggaagaga tgtaacaaga
     3181 gctgttcatt aatgatcagt tgtcctcccg atatggccag aggaaacaga caaagatgtt
     3241 cctaatcaac tatagacgaa tatggcgctc ttttttttag agcctggcat agttttggtt
     3301 tgatctgttt atcttccaag aagttccctg gtagtgctat ggagaaatgc tatttgctag
     3361 tactgaactt attggaacga gagatattcg atcctccttc gaagactttc aacagaacaa
     3421 ataagggtgc caagagcatc ctgggtatcg tgctgcaatt gtgagaatcg ttgattgacc
     3481 ctcgagctgc ttgttagctt ctgtcttcag agttgcgggg gctgattcgt aatcctacac
     3541 atgagttagt tcgtctgatc ctagatggaa acatgacagt tcactgaaag aatggaagtc
     3601 taattcttcc tcaagataaa atagttttag gaaccagatt ctacatcatt cacgaccaaa
     3661 agtatggtcg cagtccttca acagagaatg cttcgctgcg aatcggcatt gcatccaagc
     3721 acggtcttcc ccagctccga attcttcgta ttccagcttg attcaatatc ttgttcttct
     3781 gtcgcttcaa ccggcaacct gtaatctcaa ttcttctggg ccttattggt tactctgaat
     3841 tgtgagttta gagcgccaat ataagtccac cctctccagc gtatgtggca attgcaggac
     3901 ccatctttcc aattatgact tccgaatcta gcacgcgttt tcttatttca gatgcaaaga
     3961 attcagcctc ttcgagatta ttgacgtgag taatgccaaa aatcttcttt ccggattttg
     4021 aggcatcagc tatcaactca attatcgctt ctctaaatct cctgcttcct cttaccttct
     4081 taagtagctt aacttcgcca tttataccgt gcaggataat cttcatattc aaaatgttaa
     4141 ccaggctgcc ttgaaatttg ctgagacggc cgcctttgac aatattctcc actgtcgcaa
     4201 gtgggatgat tatcttcacg ttttttctgt actcttcaag cgcttcgact atttcgccca
     4261 acttagcacc cgattcagcc attctggcgg ccatcagcac ctggatgcca tgcgaaatcg
     4321 aacctgcaag agaatcgaac acgatcgcct tgcctccgct catttcggca ccaaggaccg
     4381 ctgattgata ggtaccgctg agcttcgatg atatcgtaat acatagtgga atttcgccca
     4441 tcttctgaat atcctcaaag attcctgcaa aatcggccgg cgaaggctga gatgtcttcg
     4501 gaagttcacg ggatttgccc attaatcgcc agaactcctc tggagtaatg tcgatatctt
     4561 ccttgaaagt catatcatct acagaaatgt tgagagaggc aaagggaata ccatacttct
     4621 ttaacgtttc aaccggtaga tcacatgagc tgtccgttac tatcctgacc accgtatccc
     4681 tcctctactt tcatataata agattatagg tcattacgag tcctgagcaa tggtcgggta
     4741 ggatgtcatc taattacttg aaacgatctt ggtggaaaga aagtttctaa aggccacaga
     4801 ataactaatt ccaaaaagaa agggcggaat gtccgcccaa tgatctgaat tactgtaaag
     4861 tctatctgcc agatgctatc tcgttgaacc tctcaacatc gagtacttca ggcgcatagc
     4921 ttccagcgag tctctcatac tgtctgagga atgacctcag gtggttctca gaacctctaa
     4981 gcagattttc gtataccctc gcagttctcg agtcgatgtc tccctcaagc aattcttcca
     5041 ggtccttaat atctatctct tctattagaa gaccaacttt caccgcatcc agaagagact
     5101 tgcttccact ctcgatgagt tcatcataaa gctcctgaag cgttgaattg gtgaagactc
     5161 caatttcatt ttcacctact ggatctacca tgccaaaatc accgatcaga gaaagaactg
     5221 cttctgtatg agtctgttca cttctagcaa tgttaccgaa gacattcaat ccccatattt
     5281 catggagtac tgtatagaca tcgtgagcta gcttttcttc ttctctcatg aatgcaattc
     5341 catcttcctc agtcattgag ccgctggcca gtgacattga accaataatg agaaccatta
     5401 atactgctga aaatactctc ttcatattcg atacctccat tatttggatc tcgatgaaat
     5461 attaacctct cattcttaga gtttcctgag aatccttgct tatagttcgc ttagagtcct
     5521 ggtgatatgc agaattaatc ttaagatgaa gcaactatcg aagcctggca acttagatat
     5581 atttctcagt gtttcctcga atatgaatga acggccaaga tggtctcaat ccttataaac
     5641 ggccaatcgt gaaggttaca tgtctattct tcataagagt gaatgttttt acctaatcct
     5701 caaacgccct cctggccgct ttcaaagcaa ctgccctggg caccagcctt gacccggaag
     5761 cgattatttt gttgatcagg ccgcttacca cgcttctctt tcctttttca aatgcatcca
     5821 gtgcactttt cacaacttct tcaggagaca tcgatcccgg caactgcctc cttcctcccg
     5881 aagcgacatc gaaaaactct gttcttgtag gtccaggaga cagaaccatc actttgaagc
     5941 ccttgggttg tagttcttcg ctgacggctt cgcttaagct aagtacaaag gcctttgtag
     6001 cagcataaac cgccattccg ggcacaggca agtaagcggc agtagaagca acgtttatta
     6061 ttcctccgcc aatgctgttg cgtatgagag catatttctt cgtcaagaag taaagagcac
     6121 caatattcaa cttgatcatc tgcatttctc tatctgcttc aatatcctca aagggaccta
     6181 tcaatccgaa ccctgcgttg tttatgagaa gatctacgtc tttcagctgt ttcgaaacct
     6241 cgaccaggtc gttttcattc gtcaaatcac ctttcaacgg aatagatctg actcccagat
     6301 ctctctcaat ttgatcggaa atcgtctgca atctttctaa tcttctggct acaatgatcg
     6361 tttcataccc acgctttgca agctcataag aaaaaacctc gccgattccc gatgaagctc
     6421 cggtaactag tgctctcttc aaaatgatca cctcggccaa ttataaccaa tccagactac
     6481 attgttctct cgttcatttg actttattcg ccaagaaagt tgtataatgt tggtaccaag
     6541 ttttggaggt gataatgtga agaaagttgc tgttattcta gtattggttc tggcggcgag
     6601 cgtttttgca tcttgggtgc ctctgcagga ttactctacc gtggagtacg tctataacaa
     6661 aatttcgtac ttcgagaatg gagagcagaa ggaaatgata tacggggtaa cgatcgatcg
     6721 cgatgaaaac cattttatcc tgaattacga tactacctac tttctacccc ttgacactga
     6781 gctcacctca gatatgatgt tcggtcagca gttctcgatg atgatgtaca tcttcctgaa
     6841 tccgatgcta tctttcattt atgatgcaat tgacctggaa gagcagatga acacaaagct
     6901 atacggattc ggtactctca agtacgaggg actgcattca gtagaaggaa agaatggaac
     6961 gtatactgga acaaaagtcg ctttgtacaa tgaggacgga gagttgtcga tgtactggat
     7021 aattgatcca aacattcctt tcgctcttga aacctacatg ggcgacgatt atgcagatga
     7081 ctccactaca ataactcttt gggattatgc catcaattga ttaagaattc gatcaagagg
     7141 cgcctttagg cgcctttttg tttataataa atcaactaga gagctgctgg atggggttcg
     7201 ccaagagata attcatagcg gcagctggca ggctgtgaca gttgaagaac ctgagctgtt
     7261 cttcgttcct atgaaggagc cgttagagga ggaagacatg gatttagcga gaattaggca
     7321 tgacttacat gagattccgg agattgggtt caatgagttc aagactcaag cctacattat
     7381 gagtttcctg gatcgattat ctctttccta cgaaaaggtt gcgggcaccg gaatcctggc
     7441 gaaatggaaa aaggttgaag gccctttcgt tctatttcga gccgatatgg atgctcttcc
     7501 tgttttcgaa gagacagatg ccccgttcaa atctgcacat gaaggtttta tgcatgcttg
     7561 cggccatgat gttcatatga caattctcat tggacttata gaaagaatag taacaagcga
     7621 tcttaacaga aattttctct ttcttttcca gccggccgaa gaaggcggag gcggtgcttc
     7681 gaaatgctta tcaaaattgg atcaatatga aataaccgaa gcctgggcac ttcatgtgaa
     7741 tgatgaattt cctgaaggat ctgtttacac aagagccggc gtcctctttg cgtcggcctt
     7801 cgaagtggat tgcacttttg agggaaggtc ggcacatgtt gctttctata agcagggaag
     7861 agacgcaatt gagggagcta tggattttct tcaaagggtc tattcggttg atagaggaga
     7921 ttccgtactg agatttggac tcatacaggg gggcagggtt agaaatgtgg tggcagattc
     7981 ctgcactctt tcagggaccg taagaactaa agcttttgag ctttcagaag aggcaatcat
     8041 ggaactcgaa gaacttggga tagaagtcgc tactaagaga ggtctgaagt tctctcagga
     8101 aattggttct aagtatccac aggttcttgt agacagaaat ctctacgata aactgtgcgc
     8161 cctggtcgat gtgaatccgg tagagatgaa atacgtgggg gaggattttg gggtgatcgc
     8221 tatgaaatac ccttcggtac ttttctggtt gggtacagga agaggagaac agcatggact
     8281 acataattca aggtttttgc ctgctgactc cgttattgag aagggcgttg aggttttctg
     8341 gaaggttgcc aacagctgag ctagagcctt cgaaatttcc cctctcgaag ctcaagtgag
     8401 aacttgttat cactgtaaag ggggtccggt ttggagtctt ccactaccat gaagaccgct
     8461 cctgttctta tctcagctct caaagttact tcaccggttt tttcgaataa gaaaacgggc
     8521 gtgttcatgt agcttctgtg tatcgggacg atggatctcg ctccgatctt catttgactg
     8581 aacagctcta cggttattcc cggaaaggct actgtagaga tcatagcatc gtacggagct
     8641 tcttctttga aaccacatct gccgtcaccg atcactatct gcacgttgtt cacgcggtag
     8701 cgtcgcagat tttccgaggc aatagatgca accgttctgg aggtctccac tgagactacg
     8761 cttcccattg tcagcatttt tccaagcaga cacgcgcaga agccggttcc cgtcccaaga
     8821 tcaaggacct tgttgtcttc gtgcagtttc aattcttcta tcatgcttat caagagggat
     8881 ggctgagtag aagtggagca aatttcccct gagcttgcaa gacttagtag agctctgtcg
     8941 gagtaagcct catccgctac ttcgggtgag acgaaatccc tcctgtcaag ttccattagg
     9001 gccgactcca gtctgggatc ggtcaggcaa cctgttctct tcagaagact cagaagctct
     9061 ctcatcggaa aatctgtcac actcctctag aagaagagat acttaatggc gttagcgacc
     9121 acaattatca gcaaaatcca tttcacaaaa ccgcttccct ttcttatcga taacctggca
     9181 gcaaaataag ccccgacaac atttcctgct gcgagaacca ggccgggaat ccagtatatc
     9241 atcccattaa ggatgaagat aaccagggca aaaaccgtgt agatgaagac aatgagaatc
     9301 ttcgtgaaat tcgtcctcac aagatcaaaa ccgtacgaaa aggtaagtgc tccaatcagg
     9361 aagaagccaa ctcccgcctg caaaaatccg ccgtagaagc ccactccaag aaagatcaga
     9421 gttgagagga ctctagaggg tctggaaaaa gtgagcggcg tcggtttcag taacataatc
     9481 ataacaacta caagaagcgc tactgcgatc actttctcca gtgcatcctt gggtattgtc
     9541 gagacaaaca tagaacctat cactgaacct atcgtagcag ccactatcgc cggaagtgtg
     9601 gacttttcaa acctcattcc ctcggatcga aaggtcttta ctcctatacc gctttcaaat
     9661 atcactccca gtctattagt tgcattggca acagcaggtg gcaatcctat ccacataagt
     9721 accgggagag tgaccattga gccccctccc gccataacat tcataaaacc agccacggca
     9781 cccgcgacat atattattac gaactgtaat actgtcattt gtcctgcgtc aactatacga
     9841 aggagttgtt cgagccgccc atatcaatct tcttcatcag ctttttcaga gatgtcaaga
     9901 gagaaatgaa ctcgctttga gagatattta cctgatcggc aactttcagc ggaatagcca
     9961 gtgccttgtc cttaagcttt cttccttctt cggttagatt tatcagcacc tgcctttcat
    10021 caccctgaga cctttctctc tttaggaggc cctgcttctc cattctcttc agcaaaggag
    10081 tcagggttcc ggaatcaaga aacagtcttt caccgagttc ttttacagta aggggctcct
    10141 tctcccagag aacaagcatt gccaagtatt gggggtaggt tatgccgaat tccgaaagca
    10201 acggtctgta aagccttgtg acaccccttg agctggagta cagagcaaaa caaagctgat
    10261 tgtccagttt aaggagttct tcccctcttg ggattgcagc ctcttctttc ttgctcacat
    10321 aaccgcctcc tcaattgccg aacgaaatga ctcgggttcc tcctttggct caaatcttcc
    10381 aattatctcg ccctctctgt taatcaagaa cttagtaaag ttccacttta cttttcccga
    10441 atactccttc ttcccggcac ctgaagtaag aaactcataa agtggatgga tgttctttcc
    10501 cttcacgtga atcttcgaaa acaacggaaa tgtaatgttg aagttcgtac tgcaaaaagc
    10561 ctttatatct tcatcactgc cgggttcttg aaagagaaaa tcattagaag ggaaccctag
    10621 cacaacgaaa tctctatctt tgaactcttc aaatatttgc tgcagtccgt catactgagg
    10681 agtgaatccg catttacttg cagtgttcac caacaacaat acctttcctt tgaagtcttc
    10741 cattgattta ttccatccgt caatcgtagt cagttgaaaa tcataaatac tcattatttc
    10801 acctccaata ttattgtaca ctatttagtt gtactatgga agcttttcgg cttgtttatt
    10861 tcttaagagc tgaggaatct gatttgtgaa tccttaagag cctctgcatc taactggaaa
    10921 aacccaagtc agcgttccat ctaagattct tccacattat ctcttttttc ggttctgagt
    10981 tttcgtctcc ggctgagaag ctaatgtttt tcacgcttcc gataatggag aaatggatta
    11041 ttggggcaat cgggtgtgca aagtgttata aatgaatatg acacggctag tcattagctt
    11101 ttttcagata atgaggtgaa ttcttgataa aagcatcttc aatagcggga aaggtcgaga
    11161 ccgcattagt agaggcaaac acaataatgg atgataagat caaatcttct ctatacctct
    11221 acgatggtcc cttctcatct gttctaaatg aaaactcaat acttgcagaa aagctgaagc
    11281 ttcctctgtg ccaggacaca gggttcattg aattttttgt cttcatcggt caggagatat
    11341 ctcttgagga gcccatatca gataccctca atatggctgt caggagagcg tattcaacaa
    11401 acccctatcg atattcagtt gtgaaggacc ccctaatgca cagggaaaac actggagaca
    11461 atacaccttc ggtagtgcat actttcatgg tttccggaag agaattagag atacgtttcc
    11521 tcgtgaaagg cggtggcagt gagaatctct caagactgtt catgctcaac ccgacaacat
    11581 cgcaagaaga gttcattgaa ataattgtca attcaatttc tgaaggggga gccaggggtt
    11641 gtcctccgct cagagtagga ataggcatcg ggggaagctc agaaaagtct atgctccttg
    11701 ccaagtttgc tctgactaga gagttagatt cgaggaatcc tgacgttggc tacgcctttc
    11761 ttgaggagaa gcttttgaat gagatcaatt ctctacatat cggttatcaa ggactgggag
    11821 agggaataac cgcttactcg gtgagcattg agactgcgcc ctgtcatatt gcgattcttc
    11881 cagtttcact ggctattgat tgctatctct gtagaaaggg ggtagtcacc tttgaagata
    11941 gatgatttgt ccccaggtca agaactcagc ttcacaggca gactcatagt aatgagagac
    12001 gcagctcaaa tgcgcctcaa gaagttgtac gaaggaggta aaactcttcc tgtagagctt
    12061 aatgaagcaa tagtgttcta tgccggtccg gccaagctag ttaaagaaag ctgtggagcg
    12121 atcggtccaa ccacttcact aaggatggat ggattcttgg agatgctttt cgaaaagggc
    12181 gttctagcaa ctataggcaa gggcgagaga tcggttccgg cagcgaatct gtgtaaaaag
    12241 tatagaagag tctacttgat cgctccaagc ggagcggccg cttcgcttgc gcaaagaata
    12301 gagtctctga ggaccatcgc ttacgaagat ctgggcacag aagcaatttt cgagatcgat
    12361 gtcagagact ttccgttgat tgttgcaacg gatgttaacg gttcggatct ttttactttg
    12421 aatagaaggt gattgtgtga acgaaaaggc attgaaactt cacaaggatc tcagggggaa
    12481 gctagaagta agaagtaagg ttagagtgga tagcatggag gccctttcgc ttgcatacac
    12541 tccgggagtg gccgatgtgt gcagagtaat tgagagcgat aaagaacttg cttatgatta
    12601 cacaaacaaa tggaattacg tcgcggtagt ttcagatgga acggcggttc ttggtcttgg
    12661 cgatattggc ccagaagcag gacttcctgt tatggaggga aaagccgttc tgttcaagga
    12721 attcgcgaat gttgatgcct tccctctctg tattgacatc catagtcctg aggaaatcat
    12781 ctcattcgtg agagcaatcg ctccaacctt tggaggtatt aaccttgaag atatctcctc
    12841 accaaaatgc ttcttcattg aaagcgaact tcagaagatg ctcaatattc ccgtttttca
    12901 tgacgatcag cacggggcgg cgatagttgc cgttgccgca ttgagaaacg ccctgaagat
    12961 tgttggaaag aagattgaga atctaaagat agcgactgtc ggcgttggcg cagccggagg
    13021 cgcaacaatc aaaatgctac tggcggcagg tgcaagaaat attgttgcag tcgatagaaa
    13081 tggaatacta aacaggaatc agccatctac tctcctaaat gagtttcatt ctgagatcgt
    13141 aaaggaaatt aatccagata atctcagtgg aaatctggag gaagccgtga agggagccga
    13201 tctcttcatt ggaacttctg ttgccgggct tctttcacct gaaatggtgc gcaaaatggc
    13261 tcccgaaccg atcatattcg ctctggccaa tcccgtaccg gagattatgc cagatcttgc
    13321 ccgaagcgca ggtgcatcaa ttgtcgcaac cggcagatca gacttcccaa accagataaa
    13381 caatgttcta gcatttccgg gaatattcag aggtgctctt gacacaagat caagagagat
    13441 aaacgaatct atgaagctgg cagcaactga cgcccttgct tcggctgtgc ctgaagaaaa
    13501 gctttccagg gactacattt tgccgaggcc gttcgagccg ggaatcgcaa agaaggttgc
    13561 actagccgtt ggaagagctt ctattgattc ggggtgttcg aggctctcag gtgaagtaaa
    13621 ccttgagcaa ttgatagaaa gaggattcaa gaaaatctga aagtcttgtt tccgaaataa
    13681 catacctgcg gatggaaaga tcgacttcat cgtgcacact attgcatgaa tgcagttttt
    13741 tgcatgcaaa agcgttcacc gacggggatc ccaaagcagc aaagcttctg tattcggctt
    13801 tcaatgagtg atttatacaa ttatgatctc ctggcatgat attgcttatt aagttgatgt
    13861 gatgaatact atgataaaaa ccagggggtg taaatcgtga cagaaaagtt ttcgaagatc
    13921 gcactcagaa tgaagtcgaa catcatcagg gaactgctta aggtgacttc gaagccggga
    13981 atgatttctt tcggtggagg tgttcccgat ccagatacat ttccacgttt tgaaatggcc
    14041 gaaatatcga aagaagtcct ggagaaggag tacagattta ctcttcagta tggctcaacc
    14101 gaaggtgatc cgatactgag ggaagagtat atatctcttc tgaaacggga aagtgggata
    14161 gaaggtctcg atgtcgacaa tctgcttgtc acagtcggtt cccagagcgc actggatctt
    14221 gtaggcaaga tcttcctcga cgacgatagt ctatacttag tatcaaaacc tgtttatctt
    14281 ggagccgcaa gcgccttcgc attgcgatca aacggctatg tctacatgga ccttcgagaa
    14341 gacgggattg accttgatga ggttgaaaac agattggaag agattgcttt aagaggggag
    14401 atcaataagg taaaattcat atacgttatc tctaactttc acaacccggg cggcgttaca
    14461 atatcgctgg aaaagagaaa gaggctagtc gaaatcgctg agaagtacga tgttctgatc
    14521 gttgaggatg atccctatgg tgaccttagg tacgaaggcg atccaataga tccaatattc
    14581 aaaatcggag gtcagaatcg ggtcttactt ctgaggacat tcagcaagat cctctcccca
    14641 gggctgaggt taggaatcgt tattgggaac aagaccataa taaggaaaat ggtaatggct
    14701 aaacaagcct cggatctctg cactcccagt ctcacacaga gaatcgcggc tagatatctt
    14761 cagaggcatg atttgcttgc acagcttaaa ccaacgatag ctctctacag ggacaagaga
    14821 gaccttatgc ttgccgagct cgagaagaat ttcggagaca tgaagggatt ccattggacc
    14881 aaacctgacg gaggcctttt catttggttc actcttcccg agagcttcaa tactggagaa
    14941 atgctggaaa tgggcaaggc cgagaatgtg ctttttgttc caggagaggc cttctcgctg
    15001 gacaacacat gcaagaactc gatgcgccta tccttctgtc ttcctcccag agaggatatc
    15061 atagaaggag taagaaggct gaagaaggtc attctggagt acggtaaaga gaagaatctt
    15121 ctctagagga gtggaaagat gaagattctc gctataaacc ccggctcaac ttcaacaaag
    15181 atagctgttt tcaacgacga agaggagatt gttagaggtt cgattcagca cttctcgtct
    15241 cgaaatgatc gaagtcaaga cattcaagtt agggcagagg aaatcctatc cttcctaaaa
    15301 gaaagcagca tttctttcga ttttgacgcc attgcctgca gaggaggcat attgccacct
    15361 atggaaagcg gtacatatct ggtagacgat aagatggtag attttctcct tcatcgcgcg
    15421 gcaattgatc acccttcgaa tcttgccgct cccataggtt taaggctatc tgaagggaag
    15481 attcccgtgt tcataacaga tccaatctcc gtcgacgagt tgtgcgaaga ggctcgtttg
    15541 tcaggccttc ctcaacttcc aagaatctct agacttcatg cgcttaacat gaaagctgcc
    15601 gcgagacaaa tagcctttga tcttgggaga gatgtccgaa atctcaatct ggtaatcgcc
    15661 catctgggcg gaggtatctc tgttggcctg cagttgaggg gaaagatggt ggatgtcaac
    15721 aacgcaaccg atgagggacc tttcagcccg aacagaactg gggagcttcc agtaggtgat
    15781 gtagtggaaa aatgcttctc gggagaatac tcggaaagag agcttctcaa cagatactta
    15841 aaatacggcg gacttctcgc ctatcttgga accgacgatc taagaaaggc gctggaattt
    15901 gcaaaagaag atgacgacgc agctctagtt gtagacgcaa tggcttatca gattggaaaa
    15961 gagatcggag gaatggttgc tgtagctggt ggttcgatcg acgcaattgt tttgaccgga
    16021 gggatggcat acaacactga attcgtccag aagatcaaag aatacattgg gaagttcgca
    16081 ctggtagtca tagaacctgg cgaaaacgag cttctttctc ttgctctagg agcaaagaga
    16141 gtactggaag gaactgagaa ggcaagaact tttgatccgg aggtggcaat gtgattccac
    16201 atctctcaca actcatagat cgagctaaaa ataacccaaa gacagttgcc attgcagcag
    16261 ctgaggactc aatagttctg gccgcagcta aagaggctgc agagcatggc attgcatcgt
    16321 tcattctatt tggccaaaaa aagaaaatcg agcaaataaa caatgagctt ggattttctg
    16381 aaggatttac aatagttgac tgttctacaa aactcgaatc aattgaaaag gcagtaaagg
    16441 ccgtttcctt aaaagatgca gagattctta tgaagggaaa tgtcaagacc ggggagcttc
    16501 taggcatcta tttgaaagat gagtttggtc tcaaaaccgg aagaacaatg aatctagtaa
    16561 ctgttttcga gataaagaga taccacaagc tgttacttgt aacagatgca ggtatggtta
    16621 tcgctcctga tctcaatcaa aaagccgatt caataatcaa ctcggtttct gttgcgcggg
    16681 cgctggaaaa caaaagaccg aaggttgcag ttctcgcggc aatagagact gtcaacccaa
    16741 aaatgcccgc aactattgag gccgcgattc tttcacagat ggcaaggagg gggcagcttg
    16801 gatctgtcga tgttgatggc ccttttgctc tagacaacgc tatctcacgt gaagctgcag
    16861 agcacaaggg gatttccagt tcagtggcgg gcgatgcaga tatcttgata atgcccgaca
    16921 tcgaagcagg taatatcttc tacaaagcca tggcatttct ttctgaatgt caattagcaa
    16981 gcactatagt tggggggaaa gctccgataa tactcacttc gcgtgcagat tcagcggaaa
    17041 ctaagttgta ctcgatagct ctaaacgtcc ttcttgcggg agtgatatga tgtcggttgt
    17101 cttggttatc aaccccggct cgacttcaac aaaattggct ctattcagag attcagagat
    17161 gatcggtgag catacgatca gacactcgcc tgacgaactg agtaaatttc cttcattata
    17221 tgagcagcgt gaattcagaa aggccttaat tgtaaggttc ttggaatcag tgaagcaccc
    17281 gctctcggaa atcgatgcaa tcatcgggag aggtgggatg ctccgccctc tcgaaggtgg
    17341 tacatacgtg gtaaacgaag aaatgataga agaccttaga ttggcaaaat acggtgaaca
    17401 tgcgtctaat cttggagcaa tactggcaaa cgagctttca ctcgaaagcg gaaagggaat
    17461 cccggcttac atagccgatc cggttgtagt agatgaaatg gaaccagtcg caaaattttc
    17521 gggacatccg gactatacga ggagatcgat atttcacgct ttgaatcaga aagcagttgc
    17581 aagggaagta gcatcaaggt atggaaagaa gtatgaagag atgaatttca ttgtagttca
    17641 tatgggcggg gggatttcca tcggtgctca cagaagggga agggtggtcg atgtaaacaa
    17701 cgctctaaat ggagacggac ccttcagccc ggaaagagcg gggactctcc ctctcactgg
    17761 tttgataaag ctatgtttct ctaatgaata taccaaggat gaaatcagaa aattaataaa
    17821 gggaaaaggc ggcctcatgg cttatacggg gaccagtgac tgtcagaaag ttcagcaggc
    17881 aatactagac ggagatcggg aatctgaact agcttataag gcaatggcat accagattgt
    17941 taagtgggta ggaagaatgg ctgcagcatt atccggcgaa gtggatgcga taataataac
    18001 cggcggaata gcttatgata gtagatttat ggttccgtgg ttgacagaga aactgagctt
    18061 tatcgcaccc ataaacgttg ttccaggtgg aaacgaagaa ttatctcttg ccgtcgcctg
    18121 tttgagagta cttgaaggaa gcgagaaagc aaaacaatac aagggggaac cgtgaacttc
    18181 actaaaaaga tccttatctt catggggatg tttggaagcg gaaagacgga aattgcgctt
    18241 aacgtatcca ggttactcac aaaaaaggga gaacgggtcg cgcttgcaga cattgatact
    18301 gtaagcccat actatagatc tcgcgatatg agagactcgt ttgaacagct cggaataaag
    18361 atcatcgctc caaaaggaaa attggcaaac gcggatttgc cgataattcc tccggagatt
    18421 ttcggctatg tggagaaccc agagttcagg gttgttcttg acgttggagg aaatgatgac
    18481 ggtgccgtcg tcctatcctc actgagctca aggcttccgt ctgacaactg tgaaacattt
    18541 tacgtgttaa atcattttag gccattcaat gacaccgtag agaacgccgc ttctcacttt
    18601 ttcagacttc aagaaaagtc gaggatgaaa atcaactacc ttgtgaacaa cacaaacatt
    18661 ggtcttgaaa cgacctcaga gacgatcgac aagggtgagg agttcgtgaa acaactgtcc
    18721 gaaagagttt ctgttcctgt agctttcacg gttatcatga acggcttgaa tagggaaccg
    18781 cgcttgtttg agcagcttga aattaagaaa tatatgttga atccatggga ggtagaacaa
    18841 tgagcaaaaa cttcgtgaag atcgacgaag agcgttgcaa aggctgcggc ctatgcatca
    18901 acttctgccc gaagaaagta ctgagctttt cggacaaatt caacagcaaa ggatatcgtc
    18961 ctgctcagca gcatgatctc gaaaactgta tcggatgcgg attctgttat atgatgtgtc
    19021 cagatgcagc aataaccgtc ttcaaagaaa cggtcgaggt atagggggga gattatggct
    19081 gaaaggatta tggtaaaagg gaatgaggca atggctgaag ctgcagtaag agcaggttgt
    19141 agattgtatt ttggctatcc cattactcca cagtcggaat ttacagaata catggcgaga
    19201 aggatgccag aagtcgatgg cgtcttcctt caatccgaga gcgaagttgc tgcagtaaat
    19261 atggtctacg gcgccgcatg tacaggtcat agggtcatga catccacatc cagtccgggt
    19321 tttagtttga tgcaggaagg tgtttcttat attgccgggg cgaagttgcc ggctgtgttt
    19381 gtgaatgttg tcagaggtgg ccctggactt ggtgatatcc agcctgccca aagcgattac
    19441 ttccagtcca ccaaaggcgg aggtcacggc gattaccact tgattgttct agcaccgggg
    19501 agtattcagg aagctgttga tcttatggaa actgctttca gactagcgga caattacaga
    19561 atcccggtta tgatgcttgc cgacggaatg cttggtcaga tgatggagcc agttgtcttc
    19621 ccagagtttg tcaagcttga gggaatcaac gatcattcaa gctgggcgtt aagaggcgct
    19681 gatggaagag aacctcataa agttacttct ttcgatatcg atccagttaa acttgaagag
    19741 atgaatactg aaacacacaa ggtctacgaa gagattagat caaaagaaat ccgtttcgag
    19801 actcatgacc ttgaagatgc agattacatt cttgttggat atggaactat gggaaggatt
    19861 cttggaagcg ttgttaagac ggcaagaaaa caaggcataa aagttgggct cttcagaccg
    19921 atcactcttt ggccctttcc atatgatgag ctgaaaaggg ccgcaagggg aaagaagttc
    19981 tgcacagttg tggagatgtc aacgggtcaa atggtagagg atgttcgcct ttcagttgag
    20041 ggaagcttac cagtgcattt ctacggaaga accggtggaa tggtcccgac acctgacgaa
    20101 gtcttcaagg aactcgtcag acttatagga tgaggtgatt atatggaaaa agcgacatac
    20161 aagaaaccta aatcgcttgc tgagaaggaa ttttcctatt gccctggatg tcaccacggg
    20221 attattcaca gactcgtagc agaagtcatc gatgaactgg atattcaggg aaagactctt
    20281 atggttgctc cagttggatg ttcagttttc gcgtacgagt tcttcgatat tgacggtacg
    20341 gttgcccccc acggaagagc tccggcagtt gcaactggaa tgaaacgggc aatgccggag
    20401 aaaatggtct tcacttacca gggcgacgga gatctcgccg caattggaac ggctgagatc
    20461 attcacgccg caaacagagg tgaaaagatc acaacgatct tcgttaacaa tgcgatatat
    20521 ggaatgacgg gaggtcagat ggctccaact actcttctag ggatgaagac gacaacttca
    20581 ccatacggaa gaaaagctga gactgaaggt ttcccgattc atgtctcgga gttgctaaaa
    20641 gagaccgcag gaatagtcta tctcgaaaga gtttccgtga gctcgcctca agaagtaatt
    20701 aaggcgaagg ccgcaataaa gaaggcgttc attgcacaga tgaagggcct gggattctcg
    20761 ttaatcgaag tcctatcaac ttgtcccaca aattggggac ttgacccgat agagtccatc
    20821 aaatggctga acgaaaacat gaaaaaagaa tacccaaagg gtgtatttat cgacaaggtc
    20881 ggtgatatca gatgagtgat cattttctga tagcagcggg atttggcgga cagggagtta
    20941 tgttgatcgg acagattctg gcaacggctg ggatgttcga caacaagcac acaacctggc
    21001 taccctcgta cggccccgaa atgcgtggag ggacggccaa ctgtaccgta gttgtaagtg
    21061 atgaacctat cgcctctccg ataacaaata cgccaaatga attgattata atgaatattc
    21121 cctctctcat aaaatttgag aaagagatcc agtctgatgg actgatggtg ataaacacat
    21181 cggttgttga cagagatacg attagagatg acatctccat aataaaagta gatgctaatg
    21241 ctatcgctga gaagttgggc aatctcaaag tagccaatat ggttgttctt ggagcatatc
    21301 tcaaaaagac gggcgcagtt actcttgaag gtgtaaaaaa agcgcttgaa gaaaaactta
    21361 ccggaagcaa agctgctctt gtaaacctga acattagggc aattgaagaa gggatgaaat
    21421 cagtaagctg atctcattta atgggacgta ccttagggta cgtccttatg ctgttcatat
    21481 gattgcttac gcgattcaaa catgttagtg cgctggttcg caaaatggat gtataatctt
    21541 gctaaggtat tctctggagg actaaaatgg aatctgaaaa cttctggaca gcacttttct
    21601 taacttcttt tgcgggcctc tccactggaa tcggtagcat tcttgcaatt ttcacaaaga
    21661 aaaccaatac gggatttctt tcagtttcac tgggcttctc ggccgggata atgatctacg
    21721 tatccttcgt ggagatcttt gtcaagagtg gtgaatctct ccagaaggct atgggagatg
    21781 gcataagcaa ctgggtcaat gtgattgctt ttttcggagg cataggtgtt attgctctga
    21841 tcgactttct cgtgcctaag gcagagaatc ctcacgaaat gagagacgtc gacgaaatgg
    21901 aagcaagcag taagactcta ttgagaatgg gcgtcttcgt tgcccttgca attgccattc
    21961 ataattttcc ggaggggctc gcgacatttc tagctgcctt agaagagcct tctcttggaa
    22021 taccaatagc gttcgccata gctattcaca acattccaga gggaatagct gtagctgttc
    22081 cgatatacca tgcaacgggt agcagaaaga aagccttctt gtattcgttt ctctccggtc
    22141 ttgcagaacc tgcaggcgct ttgatcggct tctctttgat taacattctg ttcaacgaga
    22201 tcgcgtttgg tgtcgtattt gccggtgtcg ctggaatcat ggtttacatt tcccttgatg
    22261 agcttttgcc ttcggcagag aagtatggta aacaccatct gtcgatttcg ggattgatag
    22321 cggggatggg tcttatggca ataagcttgc tactactgta aattgccaat catataacta
    22381 catctggcta ttctatgaga aactgcttga cgcgggtttg tgatcgctct gtgatttgct
    22441 cggttcaata agtgttacaa taatatgcga aattcccaag ctttggaggt gcttcgtttg
    22501 aactggttaa acaaagtgga agttcggtca agtacggcct gcgaccttct tcacggtatg
    22561 ttcagattga acaacaacga cagattcctc gagctcttcg cttctccgca gttcaaggga
    22621 aagtttgaac cttacaggaa catacagaca tgggtaacag aaacaaggga actattgacc
    22681 tccgatattg tagaaaaact gaaggagttt ttcgactggg aaacatttct tggaatgtgt
    22741 ctgcttccag atattaccca ttcaaatctc gagagtgcca gccatttgat cgaatttgtt
    22801 caagagatgc ctgaaagagt tcttttaatt cacttcatct attcgggtta tggacctcga
    22861 atgggcactg tcgatatgga tactttcgac aaaatcatga agaatgatag agatatgctt
    22921 ctcttcgtat ctaacgatat gtcattttcg accgaaagga aggctatcct atttgagatg
    22981 ttgtctcacc ctgaaagaac aaaggaggat ctactttact tactggagtg gttctacgaa
    23041 aacgtgttct cgacaataga acctagaata gcaaagattc attcaaaggc ggtcaatgaa
    23101 atcgaaaggg aaattagtgc tactggtgag aaatttctta ggacaatcat aaaaaacatc
    23161 gacttcactc gtagtgaagt acttgagagg accgttatct gtcccagtta tttctcggaa
    23221 ttccttattt caaacgccag tatccctttt gtaaaagaag atatgtacac catcggcttt
    23281 cgcttcaaag aagtaatggc agttcctaaa gaggccctgg aaataacagc ggaaaccttc
    23341 gatgcccttg cacacgaaaa gagattggcg ataataagat acctctcgac gggacgatct
    23401 tcagggaacg agttggcgag agcgcttaac ctttccaact atgaggtggg ggaacatata
    23461 ggtatccttc gcgaagccgg catggtgtta gccaaaaaag cgaaccaggt aacgtatttt
    23521 acactcgaca aagcaatcgt gaggcaagaa atctctagag cacttgaaga tcttcttgaa
    23581 tcgagctaag actagtgatc ttctttcagc ttacgatgca ttatctctat ctcgagcaag
    23641 cggagcttcc tgaatgactt ttcatctttt tggttcttga gaagctgtag agcaaccttg
    23701 gcaccgatcg atgcaagact tcccagagtc ttccccgttg tataccattg tttcaacgga
    23761 gtctctttga gaattatttc aaccggtgtc accgattccc ccttcattac aacactatgt
    23821 ggcctaaagc tcttcttctg cctatcgagc tttttctcga ggttatcgca catcttcttt
    23881 atgctgctca gtgaaaccag actctcaacc gaatagtcag acacaatcct cgtttttaga
    23941 gaaaaatcat ataattcgag agcatctcta atatctgctt tttcttcagc tgcttctctt
    24001 ctttcatttt gaaagctgga atgaagttga atgccccgat tcttaagttg aatacgtttc
    24061 tcatcgctca tacttctaac gcatcctttt ccgtttctgt catgattctt gtaacttcga
    24121 acttcagatc ttcgtctatg agagaaaaga tataaccata aacataatta aaatactgca
    24181 tggtagggcc atcaaaatac gaagtgttga gagatccaat tctctcttta agaatcacgg
    24241 gaagagaact cccacttttg gacagctcct tttcgttttc ggagaattca tcaataagct
    24301 ttttcaatct ctctatagcc ttcctattat acctggcgta tcttgcgctt ctaagagatt
    24361 cgactttttc ttcagccatc ctgtagaacc cgttcttcac ttctcgatac ctctcgttct
    24421 gaccttcatc tagaatagat aagtcgagcc cggtctccag tcttgccgca ttgttcattt
    24481 catcccttat agccatttca tcatttgaag ccctaattcc ttccaaaaca ctttcaagct
    24541 ctttcagaat ctcctcatta cgctcgtacc gcttacgctc gagctccata tccctttcct
    24601 ttctcagaga gtagaactcc tgctgagctt gaacaagcct ctttgaatac tcagtgacaa
    24661 ctcctatgga atttgtgcta gaagcagccc caaactcctt caatagtctt tcccctcgaa
    24721 gaaagacttc ctccacttcc ttctcgctct tagcatctct tagatcggaa aacactttgt
    24781 ccatcgcaag cttgaagaac tccagattct ccggaaactt gcttgtcgca gcaccgagga
    24841 caagcctgct ttcgaagtcc tgattgcgtt gctgtagttc cttcaagcga cgcagataat
    24901 cctcaggacc cggctccttt cgaaacttga ttgcgtaggg acctagaaag atacctccct
    24961 ctctcaatat caacctgtat gcaacatagc caagcaaaat cgccacgact atcagcagaa
    25021 aaagcatagg aatccctcct gtaacatacc ttcaaatagg ataataccat ttttcgattg
    25081 taagcgggtc aaacaagtac tgacaaccac cgacgctttt tatgaatacg agctgcaaca
    25141 gtagggacac tcgcgaaaag ccctccgatc atttaccgtg tgtaagcttt tcgggatttc
    25201 tttgccgttt tgcgaggaaa ctgcctgaaa gggccgttat cactggctct ggaatatacc
    25261 ttatcatgag ataaagaaca cccatgttga taatccagta aatgaaccag aatctttcca
    25321 tgatctctat tgggtcgcca atcagatcga agagaggata aacgagaaac cccgctgcga
    25381 tgtggtagcc aagaacaaaa agcgttctcc tgccaaatag cgacagaaaa tttctcacag
    25441 ccttgcttct ctgcagaatc tgagagagtc cggaaacggc gattattcca ataagggcag
    25501 caataacgta aaggaatggc aaagtgccga aatagtcctc catcaggtaa acttcattac
    25561 aaaaggcaga agtaattata taaaggctac aggctaatgc ggtgaaaaga aatgaaagcc
    25621 tttttgaaaa aagatcgcgg gatctcacaa acttacccaa cgcaaaataa ggaaggacgg
    25681 caagcgctac atcgagtttg aatggaagcg atccataaaa aaagtacatg gaaacaaaac
    25741 ctatggtgat cccaacaatc agtattctaa gccttctcag aagcgagtag tatatttcca
    25801 tgacaaagac ggtggggaga aaccatagag gaattatgtt caaaggaata atgccaaccg
    25861 ccgtcctaac agttaagatg tttgctatca cagaccccaa agtgaaattc atctcttgcc
    25921 tcggcactat tgctcttgct ataagccaaa tagcggtatt tgctaaagcc atggtgtagt
    25981 acggaatcat gatctgtttg aatctcttag atatactatc tttcagcggt atatccctag
    26041 atataagccc ggaaagaaaa aagaaggtgc atgctgcagg gttaatgaga ttagcagcga
    26101 tctgcggagg gagcgtatgg tacataacga ccagcgccat caaaaagcct ctggcaacat
    26161 ctatccagac aactctttca tcactagaag tgaggccctc aatccgacgt ctcgacaaga
    26221 ataaacaccc cgcacaaaat ctcatagtca gtataacaca ggcgatatgg attcattcaa
    26281 ggtacttctc tttagatgcg tgacacggtt atcggaagcc aatcagtggc tatcattttc
    26341 cgaaatatcc cctgtcttcc ttaatattgg agagaaacaa aagaagggtt aactgacccg
    26401 gttacaacaa ttccaaagtc acacaatgct gaattataac gccgccattg atatacttat
    26461 ctatataagg aattcgcgga gttgaaactt agtaaagcaa tacgaatagt tctcgaaata
    26521 agaatccacg agtttagcgg ccatctggca ggaggtgtga catatgaaaa agatcattct
    26581 gatgacggta ctggttattt catgtataac attcgctcaa caattcgacg agttgttcaa
    26641 caaccttttg ttgaagagcg aagacttcgt atctttttca ggagcgggtt tcgatgctcg
    26701 tttcgttcca acttatgctg ctaccgattc cgaaattaga ttttcggatg gtcaaaatct
    26761 gttgggcata ctcgttgtca ggtcgaccag agtgatggat atcgaaatca acaacgttgt
    26821 aataatcaac agtgaagacg actctagaat ggctttcaaa atctataaag tggaagatct
    26881 cacctctccc gtggaactac cgttttcttt gccttctgga ggagttcttg tactcacatc
    26941 agagacacct ctgcaagact acttcatgga cataagaatc cagcagattc tgcttccctg
    27001 gagagttaag acatctgagc ccgacagagt agtggagatg gctccagtaa ctttggtgga
    27061 aacagcctcg gaagttgacg tggaaggaaa cgacaactac gattctctaa ccgttagcat
    27121 ttccgatttg aacgatagac tcgacttctt gcaggacaat gtggagggac ttaatcttca
    27181 attcaggaga ctgaacactc tctttcttga cacacaggtt gcacttgccg aatacaaagc
    27241 tgcggttgat gaaggcgctt ttgacaccga agagttcgcg atgactatag aaaacagatt
    27301 gaccgctctt gaagaaagcg ttgttgataa tcccattacc aggatcgagt ttgaagaact
    27361 aagcgaatct cttgatcgct tgtactcatc attagcagaa tggagcgacg agatttcgaa
    27421 aatcgagaca ctggaagctt ctcagaccgg ccttcagtta tctatagaga atctggagaa
    27481 cacggttagg agtctgcgcg agatcacacc tcaacaatcc gattccgaag atattgtttc
    27541 gatcaacgat aagatcggcg agattactac gtcgttatca gctgctgaaa acgaactgca
    27601 agaaatgaaa agttttatcg ggttgcagtc ggaagaaatc agcacagtca ggaatgagct
    27661 tggagatgca cttgacagtc tgacagctcg cttgaacttt gtagagctct ctgcgaagac
    27721 tagcagcgaa agcatcggca caaacaatga gctgctcgcc gaaataactg ctgaagtcga
    27781 tgcactgtcc gactacgtgt cagaacaggc cctctctcag atggagaagt ttagctcact
    27841 atccgaggct ttctcagaca ttgagaagag ggtaatcgcc gcagaaactt ctctgtctga
    27901 ttttgaagag atcaaggcca ggctttccgc ttcggaatct gcaactcgca ttctctctga
    27961 cagaataagt tcactagaag ccttttattc ttcagcagaa gatattatcc caatactcga
    28021 ggaaaaggtc ggaaatctcg aaaccagtgt ctttggaaca ggcaagataa atatggatga
    28081 acttgaaact gagatactcc agctcaggga aattgccgac agtttgtcac ggggctttgt
    28141 aaaattcaac tccgagctga taaccttgag agaatctatt cctcccgaag gagtatcgat
    28201 cgaaaccttc gaaaattcca taaatgagtt atccgacgcc atcataaata tcaacgcaga
    28261 cctctccgaa ataaggagtt cagtagctga gctggaactt tcactagggg ttcttgaaga
    28321 ctctcttgtc ggagtatctg tgggtcttga cagcatgaag ggaaatttcg acggctccgt
    28381 tgcagcaata gatagcaacc tgaaggcgat tcaaaagatc gaaaatgact tgctgcttac
    28441 ccaagaggat cttgcaaaga ccagagatca gctgtcaact ctgacgaaag aactagaaaa
    28501 aacactggtc acccgggagg aaattatcac aataactgaa gaagcagtaa aacaatctag
    28561 agaagccacg gcgcaagaga tctcttcact gagaagaaca aataacatct ggttgactgt
    28621 ggcagtaatt tcctctctgg cagctatcgt gctgggagtg gtaaatatcc ttccttaacg
    28681 ggaagggttt cgcgagaatg aaactgaaaa gagaaatcct tattattgcc acttcttgtg
    28741 tcttgctatg cccgacaaat ttctctgctt attcattgtc ggagattgga gaacttgcag
    28801 agcttcttaa gcaattattt ctggcctcaa gaacggccag agatttctct cttgagagtc
    28861 tcaaagtcga taccgaagaa ttccctgcag ttgactttgt ggttaaagag gatgtcgttt
    28921 attcggaagt tgatggagag atactcacaa tggatgttta ctatcctgcc agaactactg
    28981 ctccaagacc tgcaatactg tatgttcacg gaggcgcctg gattactgga gacaaaagga
    29041 gtggacccgg cgtagtagtt accggtgagc ttataagagc cggattcatc gtattctcca
    29101 tcgactaccg gctcgccccc aaatggaagt tcccatcgca ggtcattgac gtaaaaacgg
    29161 caattcgatt tgtaagagaa aattccaccg aattactgat tgatcctgaa gcaatcggag
    29221 gcttcggaac aagcgccggt gcacatcttg taacgcttgc tgcattgacg gcaaataccg
    29281 gcatgttttc tgaagacaaa tactcaggtc aggatgaaaa tcttttctgt gtcgctgatc
    29341 tctacggacc caccgatctt gaagccctat tcgaaggtat tgagagagag gtagcggagt
    29401 tgatctttgg gaatgaagag ggaatactta gaaaggcgag tccaatcaac tatgtaaata
    29461 aagattcccc accatttctg atagttcagg gtgatagcga tcttgtcgta ccggtcgatc
    29521 aatcgaaacg cttcttcgaa gagctcactg ctgccggatg ctatagcaaa ctggtggtgg
    29581 tagaaaacgc cgggcacggg ttggtgcccg acggaggaga aatcaaacct tcacttatag
    29641 atgtcgcaaa aatagttgtc ggcttctttc aaaggcaaat gattatccaa caatctcgga
    29701 taaatcttct tctacggttc taataggctt tatatcgaag ttttccacaa gaactttcag
    29761 aacattcggt gaaatgaaag ctggcagtac cggacctaaa cgaatcccct ttactcccat
    29821 atatagaagc gcaaggagta cagctaccgc cttttgctcg taccaggcaa tatcatactc
    29881 gattgggaga tcatttatat cctccagacc gaaagcctct ttaagcttca aggcagtcac
    29941 aaccaacgag tatgaatcgt tgcactgccc tgcgtctact actctgggaa tgcctcctat
    30001 gtcgcccaga tctagcatgt tatacctgta ttttgcacaa cccgcggtta atattaccat
    30061 gtctttaggc agcctctcgg ccaggtccgt gtagtactgt ctctttttag catgtccatc
    30121 gcaaccgccc attaccacaa acttcttaat tgcacctgtt ttcactgcat caattatctt
    30181 atcggagacc tttgacagct gatcgtgggc cagcccaaca acgattttct tgcctggtct
    30241 agctccgata tcgcctattt caagagcctt ttcgatgatc ggcgtgaaat ccttctcttt
    30301 cccgtcaact ctgtttggga tgtgcgatac ctcgggccat ccgacaagac cagtagtaaa
    30361 tatcttgtcc ttataagatt ccctcggcct ctgaatacaa ttcgtggtca ttagaatggg
    30421 acccttgaat tcttcgaatt ccttctgctg attgtaccag gacgttccga aattgcctct
    30481 gaggtgagag aactttttca gtccgggata accattcgca ggaagcattt caccatgagt
    30541 atatatattt attccctttc cctcggtctg tttcaggagc tcttctagat caagaagatc
    30601 gtgcccacta accaatatcc ccggtccttc atacgtgccg gtatagactg acgtgatttc
    30661 cgggtttcca tagctgccgg tgtttgcttc gtccagtaaa gccattgcct taactgcata
    30721 ctcgcccgct ttcaaaacta gcgcaacgta gtcttcagct gtcaatgagt cgttaagaca
    30781 ggaagcgagc gcctcctgaa gaaagtctag tatagaattg tcagagtgct ttaggatata
    30841 ggcgtgatct gtgtatgctg caattccttt taggccgtag gtgagaagct ccctcaacga
    30901 acgaatatct tcatcctgcg tatcaagaac acccacttga gcacctttgg ccttataaga
    30961 ctccactcca cgtgatgact cccaagtgca tgcttcaggc atcggttcgc tgaattcctt
    31021 tcctttctct tttctgtaag cctctttgaa gattctttcg attcgtcttc ttacattgag
    31081 ggccctgtca atcttcttac ccagactctc gggatcaaaa tccacatttg taatcgttgt
    31141 gaagaggcct tcagcaacat aaagaccggt ttcagaatct ttcactccca tttcttgcgc
    31201 tttgacgccc cacaaggaaa tccctttcaa aatccatatc agaagatcct ggagatcggc
    31261 tacttccggt tcctttccgc agataccttg gacagtgcag ccagtaccct tcatcgattc
    31321 agaacactga aaacaaaaca atctaatccc tcctttactg acagtaatta tcttacagac
    31381 gcccagaggg agaatcggta acatatgtta cacaggagat cgatcaataa tatgtgtaca
    31441 cccttgagta aataaattct tcgggagcgc ttgagctatc caggaactca attctcctaa
    31501 gttcccgaac agggttgcct tgttcgtccg tgaggagtat atcgattcga cttctgaact
    31561 ccgaagcctg ttccctgcct tcccttctaa ccctaactac tgtcttctcg cccaccaaat
    31621 gtccattgga atcataagtg aatgtgcttt ccactactat gttaacctcc atatctagtt
    31681 cagaaacgtt ttctcgaatg agggaatacc tctctgattt tcttccgttc tcatcgaaga
    31741 agagagtatt cgttgaaacg ccagtttcgg tttcaacaga aaagatcaga gttctttctt
    31801 cgctgttaat ccagtagcga gttctttcga gttcaatacc tgaatcgtca aaggccacgg
    31861 catcgtagtg atcatcttca tagagcactt caaccctctg gaaaactgta ccgtctcggt
    31921 tgatgaagtc ctttcttctc agtctgccgg agtcgtcata gaagtattct atcttcactt
    31981 cttcagtctc cctcgagatt agaaaaccct tttcattaaa ggtcagcgtc tccggatcgg
    32041 aaatcaatag ttctgctcct aactttgcta tctctatctg tacagtatgt acctttccag
    32101 aaaaaccaaa ctgtaaagca gacagatctt cctccataaa aacggcaaaa agcgagctta
    32161 gaagcacaaa gaaagacagt gcaatcacga atacttttct catactctcg cctcactcac
    32221 tcgaaaatag aatctaagac aatcataaca aatactgatc tagccggaat gaaaaaaccg
    32281 gcagtgcctg caaagtttct attaaatgtc aattcttctg atatactgag agtgatgatg
    32341 aacaagcttc tcacggggtg atcactgtga ctttcaaaga actgtctgag cgaagtggta
    32401 ttaggtacga aacggtacga aactatgtga aagtcttaat agaagaaggt cttatcgacg
    32461 aagtaagcga agaagttgtc gagatcgtca agaagatgcc tgactacact tctcaagggc
    32521 tcacagtagt cgaagccgca cacagagcgg ttagtggcga gcaaaaagat agccgcacat
    32581 ctaccgagac tctgaagctt aaagagagaa ttgcatgtct tgaagaagaa aacaaattgc
    32641 tgaaggctga acttgacaaa gaaagagctg cgacaaaaca gcttgaagag aaaattcaat
    32701 ctattgaagg cggtcagact agttcatctg gaatagcagt ttacagggaa gaggcaaaga
    32761 ctgccgcaga agcactcaaa actgcaataa aatccgctgg aagcgggttt gttcagttcc
    32821 ttcagtggct atttggcgca gaagccgaaa aagttgaaga gaaatcagaa aagtagcgca
    32881 ttatattatc gttgtgaaac aaattcagga gacgcttaga atgtctcctg aatattgtgg
    32941 gtaatactct ctataaacta gaaatcaagc gtcgctagaa tcccggtgta ccactctgcc
    33001 cacataaccg cctcttcgta gtctgccaat cccagaagga caaagtattc tccgggacca
    33061 aagaactcag catcatgtga ttcagaatag agggccatcc agcaaagctg tcccgaaaag
    33121 tcgagagcat agacgttcac tacggactcg cgatccacat aaccagtata actatccacc
    33181 agctcatagt caccgaaact acctaggtac tgatagacgt attgatttag cacgtcgctt
    33241 gtggaagctg actcttccga ctcaccaacg aagatcttgc ctacgactgt gccctgaaat
    33301 cccagatcgg ccacttcaaa gtagtctgcc agatagaagg ccacggaaaa ctccggtggc
    33361 atcatgaacg cgaagtaatc accttcaact tctatccagt cgtcgatata tatatcgagg
    33421 tcttcttcgc tcagcaacga ttcaaccatc ctgacgaaaa tatctgtgtg ctcctctttc
    33481 tcagaaggca atgcaatgac ttcttcttcc tcttcaggtt cttcttcagc cgagataagg
    33541 ctccccacga ttccggcaat tatctcctta gctttgtcaa gctcgcttcc tgaataatga
    33601 aggggctcaa aaacacacat tgtaactgca ccgggctcag ttgtatctgc ctcttccgat
    33661 ggagagtaaa caactacgaa tggataatcg acttccggtc tgattttgag agaaacaagg
    33721 taagcaatcc gtccgtcatg cagttcaaca gtctcctcgt agctactctc agttgttgcg
    33781 ccttctccat agagagaacc tacaacggca ctaatcagct ctgaccgggc gtaagactcg
    33841 agctgataca aaaatagcct tccaaaaaga agcccttcct tgtcaaaaat ctgcacatcg
    33901 tagtttagac cggtcttctc ctgaaagatt gagaaatcct ggggaatcat tatctccgta
    33961 gaccctatca tctccacttt ccaggaggga tcgtcgggag acgtccatcc ttctctgaag
    34021 gaaacaagca gaggatcaat ggttctcaga gaaagtgtat tcaactcctc actatcgggt
    34081 acaatttctc ccgtttccga cgcatcggca gtctcagttg gttctggagt cagggttctc
    34141 agtccgagct gcccaaaaag tccgacagca aatattacag tcagtgcgat gagcaagagt
    34201 ttcttcatta ttacacctcc tgattcttct agactcggat atcgaaactg gttcagaaga
    34261 attgtactac caagagaagc tttatgatac ttttgtcgag tgactctcca gaaattcagg
    34321 ccgtcctgtc aaaccctttt gaagaatagt agaatctcat tcctacatac tcaagcgcct
    34381 ttctcaaggc cgagtttatt gaataagttc tcgagcccat gtgaggatca attccctgta
    34441 cccgtttcaa ataatctgag aagactggaa tctcaagccc tgaagaaaat ttctcggtga
    34501 acctttcaac agaaagcgga tagcccgttt ttacgtattt gcagatcacc ggggccaccc
    34561 gacccaacct atcgattact cctgagaagc caaagagcct gcaaattagt ggcctaaaag
    34621 catattcggt gcaaccacct tcatttctaa tctctccaga accttcgaag agaatgcagc
    34681 gatcactgtc aggaacatct tctagccttc gaagtaagtc gtctgctcta ccctcgttcc
    34741 agagtctcag cgaaagagga agaaactctg taattgtcac ctgaatggag tcaccggatg
    34801 tcttacaaca ctctctacat gaaggcaggc atcttaaacc agtctctatc tgaatatctc
    34861 taattgttct acaggcatcg ttataaatct ctagaacctc agaagccttt tgatacagat
    34921 catcaaacga taaaccgctc atcaaaggta ttacctcccg caaacatact gagaagatca
    34981 tcccaccttg cagcgaacga atcaattcgt cgaaagtaaa ttctccatgg atataccgcg
    35041 atcgagaggt gaatttattc cgtcagaaag aagcaaactt gttgacattt tgattgaaat
    35101 ttcctttgtt gttcacggat tttgaaatcc acaattcagt gaactatgga ccttaactga
    35161 caaccggaga cttcgtcttt ttcggcagtt agtttcgaag gcatttcaaa gacttttgac
    35221 agaactccaa gaaaaagtac gtataatcat gatctctaaa gaggagagct gaaagccgct
    35281 cagtatacaa ctgttttgac tcgataacaa aggaacaatc gatcggaaag tatgaaaata
    35341 aagttcaagc agtgcaaata gatttctccg tgcttcaaaa agaagcgagc aaccaattat
    35401 gattgctcgc cttgctctca gagggttaag attactttcc cgccgaaatt atcgcccagt
    35461 tcaccagcct ttcaagatac tcctgatttg aaggttctgg aagagggttc ttcttgtcga
    35521 ttatgtattc ttcaagcgac ctgaagtctg ctatgccttc ggtgatctcc ttgcctacac
    35581 ccgattgata actcctgtat ctgtctttca agagactttc cagggtacct tcgtcgatta
    35641 gtttggctgc cgccttcaat cccagagcaa atgtgtccat accggcaatg tgtccgataa
    35701 atagatcccg ctcatcataa gaacctcttc tcacctttgc atcgaaattc aagcctcctg
    35761 gatttaatcc tccgttcttg attatctcgt acatcccaag ggtcgtcgaa tagagatcgg
    35821 ttggaaattg atctgtatcc caccccagaa gagggtcacc ctgattggca tcaacgcttc
    35881 caagcaaatc gttgattctt gcaaacctta gttcatgctg gaaggtatga cctgcgaggg
    35941 tcgcatggtt tgcttcaaca ttgaacttga aattctcttc tagaccgaat tttcttaaga
    36001 aggccagtgc attcgcaaca tcaaaatcat actgatgctt ggtcggttcc ataggcttgg
    36061 gctctatgag gaactgccca gaaaaaccta tctctttggc gtaatctgct gccatgtgga
    36121 agaatcttgc aagattttcc agttctagac ccatatcagt gttgagtaga gtctcatatc
    36181 cttcccttcc accccagaag acgtagttct tcccatcaag ttccttcgta acctcgattg
    36241 cttttttcac ctgggcagcg gcgtacgcaa agacgtctgc atcacatgat gtcgccgctc
    36301 catgcataaa cctgggactc gagaagagat ttgccgttcc ccacagtata ccgacattac
    36361 tcgtccttaa atgatcggaa atatgtctca caatttcgtc tagattcttg ttcgtctctc
    36421 tgagagtctc tccttcagga gctatgtctc gatcgtggaa acagaagaag tcaatactca
    36481 gcttctcaca aagttcgaag acggcatcaa ctttcaattt tgctagatca atgggatcct
    36541 tgatggaatc ccagcttctt ctcattgtgg gcttcccaaa catatcaagc ccatcaccct
    36601 gaaaggtgtg ccagaaggca accgcgaatc gaagatactc tttcatggtc ttccccatga
    36661 actcctcttc tggattgtag aagctgaagg ccattggatc tttgttatcc gggcccttgt
    36721 actgaatctt ttctattttg tcgaagacgc tcattcccct tcctcctttc atttcgtgat
    36781 actttcctgc aatttactta tcttcttgta gctgtctctt atttttggat acaactcttt
    36841 gtatattgag aaaacattgt cataaaccgc cctgacatcc ccgttcggtc tgaattcttt
    36901 cttcatcctt atccatgatg aagtgcctat gccaagagct tctgcagcca ggcgagccga
    36961 accgtaagct gccccttcat ccacttccgg gagccaaatc gtcttgccaa gattgtcggc
    37021 aacgatttga caccacgttt cgctccttga accgccgcca acaattctga cgttattcat
    37081 ctctgatccg agactctcga tcagctctgc cccttctctg agagcaaagc ccactccttc
    37141 atatatgctc cgaaatacat cgcctctgct gtgaaaagag gaaaagccga aaagcgttcc
    37201 tcttgcgctg ggatcacgat gaggcgttcg ctccccattc aagtacggca aaaaaaccac
    37261 tccatctgat cctgcgggtg tctgtttaac catctcttca agtgattcaa aagaaaggtc
    37321 gtttccaaat ctatctttaa accagtttag cgaattcgtc gctgaaagaa ttaccgccat
    37381 atggtagaaa tccctacctg tgacatgtct aaaaagatga attccgccag atctatctgg
    37441 tatcccccgt ttagtcaagg ctaccactgt accggaggtc ccgatgctta tcattgcgtc
    37501 tccaatcttc tcgacgccgc aaccatacgc cgacgaggca ttatctgctc ctccgttaac
    37561 taccgggatg ccttctagct ccgaaaggga gggcgaactt actgtcccca cgatcgtacc
    37621 tgacccgacc aattcgggat atgaacgagg atcaataccg attatttctg gaattctctc
    37681 atcgaattcc ccttcaatat ccagcatgga agttcctgaa gcatcggaga agtcagttac
    37741 aacacgtccc gtcaatttca ttacgatgta gtcttttgga agcatccatt tcttgatctt
    37801 cttgaacctc tcaggctcac tctctgccaa ccagaggatt ttgggaagcg tgaatcctgt
    37861 cagtactgga tttccgaaac ttcctattac tttttcttct cctcccaaca agtccgtaac
    37921 ttttgcacac tggctttcag ttctctgatc gcaccaaagg attgccggat agacaacctt
    37981 cccttctgca tcaacggcaa caagggaatg catttgaccg ctgactgaaa ttgccgatgg
    38041 tggtccacct cctttagcaa ccagttctct caaagcaatg caaactgcgt cccaccaaat
    38101 ctctggatgc tgctgtgacc agccgggctt cggggtttcc ataggaatag ggttcgaaga
    38161 agaagtcaag attcttccgc tctcatcaac cgagattcct ttaacagaat ttgttcctac
    38221 gtctataccg aaaaacgcac tcatggcaaa tctcctttat actaaatcga ccgtcttacg
    38281 atcaattatg tgtagagctg ctcctatcat ttttgagctt gccatgcctt tttcgtggag
    38341 catgctcctt attactttca cgttctgccc agtagcgtat agaacccttg agttgacaaa
    38401 atcgagcaga ttgcgcaatg atacttccgg aagtatcgaa gcaactccgc caagaacaac
    38461 aacttccgga tccaaggaat ttacgatgtt aacaattcct acgccaaggc ttctctcgat
    38521 ctcacgaaga agtggttcat aacttgcatc tgttgagcgc agacagttga gatacttctc
    38581 gttgttactg gttcctttga gttcaagccc caactcagtt gctagattga cgatggcttc
    38641 agtagaggcg atagtttccc agcaaccaaa gtttccgcag tagcatttct tgccatcagt
    38701 ctgaaccgtc atgtggccaa attcgccggc agaataatcg cgccctctat aaatctggcc
    38761 gtcaaacata acaccgcacc cgattccctc agatacatag acgaagacaa tatctcttac
    38821 tgacactaca tctggattgt tccacttctc agcaatcaac gagagatttg cttcattatc
    38881 aagaaaaacc ggataccgtc gagagagccg ttctctgagt acgagatcct tccatccgag
    38941 atgaggcacg tatacgattc tggaattctc cacatcaacg attcccggaa ctgacaacga
    39001 gtatccgagg aatctggatt ttggaatatt tctcgaaatc aaggaagtct ttttagttaa
    39061 gagatccatg aactgatcaa agtcacgtgg agttctaaat ctactcacaa ctgaaactga
    39121 gccatcaaag aatcccttac ctataattgt ctcttcgaca ccaatatcga tgactacaga
    39181 catgaacgcc tttctgtttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    39301 attgtctctt cgacaccaat atcgatgact acagacatga acgcctttct gtttagtcta
    39361 aggacgattg cctttctccc aattcgaccg gtctcttctg ctctcacttc ctcgcaaaga
    39421 cccattgata gaaaatcctt gattattttc gtcaccgtgc ttgggttcaa ccccgatgct
    39481 actgcaagat ctcttcgagt agtagcggga ttctttctaa gcaactgaag aatcagcttt
    39541 ctatttgatc ttccaatatt ctctgaatcc agctttttac caaccaagct gaaatccccc
    39601 tttctactct agcttactat taataagaga tttgattaaa tctaaacaga accgtttaga
    39661 gacagctggt ggggaatatc gcccataaga tgattcttcc taatttattt attgccgcaa
    39721 acaattgaaa gaatcttctt cgcccagact ctaaaaaact ttccgaccaa gcagcgaaac
    39781 tcaagaataa cggacaagtg attcttactt gtagcctgga cggatttgaa tcactagtat
    39841 gattctatca gctaaatgaa tgatgctaac aatgaaagtt actgccagtt ctgagaattt
    39901 ccagaatctg tcggtttttt tgccgatcat tcagattatc gaacccgaga ctcttcaaaa
    39961 gcggtttgtt ttatggtcat tcatttctgg cataaatggt cttacttgtt atctttgaat
    40021 tgaatgatca acaagcagtt ctttagtata attgtctcaa ggattattac aaggaggtcc
    40081 tgcctgtgct tctcgaacga atcaagtata tagattggcc tgcttatgat tttcttcttt
    40141 gtcttatgag ggtcaactgt aatgagatga taatcgaagc ctacaaagaa caagatttca
    40201 aagatcagtt tgtgcctgac ccagagctcc ttgaatgggc aaaggaaaca aagaagattc
    40261 ttcctgacga tataaaagag cttctcgagc gattttttga ctgtgaaacg tacttcggcg
    40321 caggaacctt ttctcttgtc caggagagaa atctacgaga acccgaggag ttcatcaaag
    40381 ctctcaagag cacatccgag aaaagcatac tagggcgttt cctaagaact ggatttggaa
    40441 ccgatgtcaa gtctagagac tcttctcaat tcgaggaatt agttgaaagg ctgacggtag
    40501 atgaaaagga gatgctcctt ttcataactg agagtactat tttctcgtct tttcaaaagg
    40561 caaatcttct tggatttctt gaaaacccgg ctgaaacaaa acaggatctg atatatctct
    40621 ttgaatggtt ccttgaaaat gttttcaaca gaatgcggga cgaagttagg aggtcgaacg
    40681 agaagcatct gaagaacctt gaggaataca ttcgaaaggg gggcacatcg tatattgaaa
    40741 aattgaacct cttgagtttc tttgacatcc tcgagacggt tcaaactata gaattgagtg
    40801 tatcgccatt cctgggccta gatcaagccg gccttatggt tatgaaagac aggtacctct
    40861 ttattctcgg atatgacaga atagaagtcc ccttcaggga gaaagatgag aaactacagt
    40921 gtatcgacgt ctttagcgct ctggcccaca aagaaaggct tgcaatactt agagaactta
    40981 gcagagagcc tcatcgacca gtttcccttg cgaaaaaatt gcgaatctcc gccggcgacc
    41041 tgagcaatca tctcgaaaag ctcaaaaagg ggaggctcct tgaaagcgtt tataatggtg
    41101 agtatgttga atacacagtc aatgcaaaag aactcaagag gcttgttagc gaatcgcttg
    41161 ataagctgct tgaagagcag aactaaatcg aacaacgatc agatcaaccc tatttaaaca
    41221 gaaacggggc tcttcagccc cgtttatatt tgaactgttt cgcctatcgc tcgatgaaat
    41281 cttccagaat cttcacaaac aactccgtgt cctcaagaat aacactgtgt ccgtagtcag
    41341 gaagcacacg gatttcaaca tcctggagta ttttctcgaa tctccttgcc atctcttcag
    41401 taacaatatt gtcaagcttt ccaactataa caaggtgtgg aacctcaatc tcactgagcg
    41461 cttcagtgaa atcgtacttt tcaagcgctc tagcgttctc tacaaaacat cttccatcca
    41521 tcatcagagc ctcgtcgaca agctgctcaa gaaactcgtc tacttcacgg gtcggcatta
    41581 caccaatcag agccttttta agaagctctc tatttcctct gtacatctcc agatatgggt
    41641 aaacctcttc tggagtcttt agaccatcgg gaggggcagg atccaccaaa atgactcgat
    41701 cgatcagatc aggcctcttt atgactgcac tctgagcgac agctccccca agagaatggc
    41761 caacaagaac cacaccatct aattccatct caagtatgaa attaatcacg tagttggcgt
    41821 aattatcgat actcacctct tcaagtctgt cagatctccc gaaattcggc agatcgattg
    41881 cataaccttt taatccactg gggagaatct tgagagcagg ttcaaaccat ttgctcgacg
    41941 cgaaattacc atggactaat accacgggaa tctcatcgtc acccactgtt tcaagataga
    42001 agatcttttt gtcaactatc cttacgtact gttcggctat tgacctttcc atgaccagat
    42061 ccctcctgtc agacttgtgt gttctgtgtt cctcacttct catattaaca ttcaaagcac
    42121 agttcgagga aacctctttg gtttcacagg cgaaaagtct ctcagtccga aagaaatttg
    42181 ccccaagtgc gcatattaca aagaagacta tgagcaactt ttcaagtata cacactcacc
    42241 tctcaaataa tctccttttt acaaacccag gtatgagcgc ttcacgtgtt cgttgtgcat
    42301 caggtcatca gcagtgcttt caattgcaag ggtcccattt tctatgacat agcccctatc
    42361 aatgactttt aggccctgtc gaacgttttg ctcggaaagg atcatggaaa ctccctcgga
    42421 tttgatcatt gagagtttct caaagagctc tgtaaccaaa gtgggctgaa gtcctactga
    42481 tggctcatct agaattagaa gccgtgggac tgccataaga gatcgtccaa ctgcaagcat
    42541 tctctgttgc ccaccgctca ttgttccagc cagttgatag gcccgctctt tgagtactgg
    42601 gaaaatttcg aacacaaatt ccagcctctc atttactttg tctctagctt cctttatata
    42661 ggatgcaccg atcttgagat tctctaaaac agtcataatt gggaacagct caccatcctg
    42721 aggcacaact gaaatccctt ttcttacgat ggagtgtgtt tcataaggaa gcagcgattc
    42781 gccttcgaag agaatctcac ccgaccttgg ctttacgaga ccaatgatcg agttgatcag
    42841 agttgtcttt cctgcaccgt taggaccgaa aagcccaaca gcttccgctc cgacattgaa
    42901 ggagaagtcc gtaattacct gaatcctccc gtatgagaca aatagattct tgacttcgag
    42961 catcaggcat cctcccccaa ataagcagtt atgacgttgt gctgttgcga tacttcactg
    43021 taaggtccct ctgcaaggat agtaccccta tcaagtacta taactcgctc cgttatttct
    43081 ctgattacgc tcatgacgtg ctctattatg catatcgtca cgcctgttcc tctaatcttt
    43141 cttacggtat ctatcatttc tctggtttcg tccatgttta atcccgacat aacctcgtca
    43201 agaaagagga tcttcggttt tgtggccatt gctcttgcga tctctatctt cttgatatca
    43261 agaacagtta tcttatcaag agtttggtcg ggctcttcaa gtcctattgt ttcacaaagc
    43321 ataagagcgc tctttttgct ctccttaagc ttttcaccgt ttccaaaaag tcctccgacc
    43381 attatgtttt caactactgt gaggttcttt aacggtcgag caatctgaaa agtccttgcg
    43441 atcccaaggt tgcatctttt gtgtgctgga agaaaagtga tatcgatgcc gtcgaatata
    43501 attttcccct cgctcaccat atggactccc gatataagat ttgttagagt cgtcttccct
    43561 gcaccgtttg gtccaatcaa accgagaatt tcaccctgct ggatctccat agagacgtta
    43621 tctacggcga ctagtccacc gaaacgtttt gtgactttat caagcttaag tatactcatt
    43681 ttatcgttcc tccagacggc gttttctgaa tccagtaaca tctttgattg cgccatacac
    43741 accgttcggc aaaaagagaa ctaccacaac tatgattaca ccgtagataa tcaaatgtcc
    43801 atagggcatt attaacttga gatactcaga tgaaagaccc agaataatcg cgccaaggac
    43861 tgggccaata gttgagtata ttcctcccac caacgccatg gctatcggta gaagaagata
    43921 atgagctgaa aaactgctct ctggataaac aaatccatac tgaagtgcat atgttgaacc
    43981 tattactcct tgtattatcg aagtcacaac aaaaacaaat atcagatatt tgtagatatt
    44041 tactccatga gacttagcta ctgtttcatc ggctctaata gaatcgatcg caaaatgaac
    44101 tctagttctc tgaaacactt ctgaaaccac aattgtaccc actgtaatac ccaacatgag
    44161 ccaataaact ttgacagagt cgtagttaaa aagagttgta taaactatct ttccacttgg
    44221 gcctccggta agcttgttca aattccttat gattaccatg aatacgcttg cgagagccat
    44281 tgttgttatt gcgaaataca aaccacgcaa tcgcagaatt gccataccca gcaagagaga
    44341 gacacctcca gccaaaagac ctcccaggat aatccctaca agtgggtcca caccgaagtc
    44401 agcgactgca atgatacccg tataaccacc gagtccgaaa aaaccagccg taccaaaaga
    44461 cagctgacct gttctgagca tcatatccca gctgatcgac aacgtcatga acgtcaagat
    44521 agttatggca acacttatga agtacgcctc agtcacaacg ggaagaagag cagccgcgat
    44581 gagcaacatg ataataacaa gtgactttgt gatcttcata atgattgcct ccttagggat
    44641 ctgatcatta tgacagctat cagaactcca aagaatacta tgtctgacca gcccgcataa
    44701 ccaggtatag attgcacaat agcttcccca actccaagaa caataccact aacaagaatt
    44761 ccggtaagat ttccaagtcc agccattgca actagacaga aagacttcat tgtaaatgcc
    44821 gaacccacag aaggaaagat tgcaaatctt ggtaccatga ctcctgcggc aattcccaga
    44881 atagcaatcg atattccaaa cacaaagaga tacacgaaac ccgtgttgat cccaacaatt
    44941 ttcgcgccct gtggattctg accgacggca aaagttgcct gccccagtct cgtcttcttc
    45001 agaaagagct gaagtgcaat tagcactgcg aaagcaaaaa caacatacaa gagctcatag
    45061 gtgccgaatc ttacacctgc aattgttgta gacgaagaag agtactcaac gtagacactt
    45121 ctaggtcttg atgtccagat gacattcatt gcttccaata aaatcatcga tatcccaaat
    45181 gtgagaagca actggttcaa atgtcctgac ttcattactc tcgtaatagt caacttatag
    45241 atcaccactc cgagcccaaa caatagaaaa aagactggga taatggacac gagaggatcc
    45301 attcctaggt ttgtggcaaa aagatagaca aaataaatac ccagcgagag gaaatcgccg
    45361 tgggcaaggt tcataattcc tacaactcca agggttaacg aaagaggcaa accgaccaaa
    45421 gaatataggc ttcctctttg gagtccatat atggcggcgt gaggcttcca aatagccacc
    45481 acgacaatta cagcaatcag gagaaagaga ttcctgtgct ttctcatgaa cgagatgctc
    45541 tccaagtcag tcaccaccca agctacagct tattagatag taaggtcggg cacgagtgcc
    45601 cgacctcaca ctacttatct tccttcccaa gaaggaaatg gataaatcgg ttcagcggtc
    45661 ttgagattaa gcggccaaat aacctgctgt tctccgccct gccactgaag tattttctgg
    45721 ccagtgaatc cctgatgctt tattacattg cttggagaca tctttaggac ttctcctatc
    45781 ggtgaagcgt attctacttc ttctagcgcc tttatcagtt ctgccttgtc aagagttccc
    45841 gcggctttga ttccttccgc aaggaagtag atgtttgtat atcccagtgg tgcgaaataa
    45901 gttgccggtt cttcattgta cttttcgatg taagcattcc agaagtgctc tgcagttgga
    45961 cttaccttgt ttattgttgg tgcccacatt ccgtagagtg tcacgccttc tgctagagga
    46021 gaatctccga agtgtgccgg ccaacctgga ggtgctccaa caaagaactt aggagcaaaa
    46081 ccaatttcct tggcctgctg aagcattgga agagcatcgg catcgtaacc tgcccagaca
    46141 aagaggtcgg ggtcataggc cttagcctgc tgaagcattg ctctgtagtc gcctccacca
    46201 agcgccgcac tcttgaagga gactcccata tagtctccca tgacagctgc ccacgaacct
    46261 tctccctgat tggcctgaga ttgaagtaca agataggatt cgtgagaggc agatccaaaa
    46321 ccgccttctt catatgcaag gaagatcttc gaaatttcga tgtcgggata agcctcagct
    46381 aattctgtcc aacccaggcc gtagctggca ccctgctggt agtcccatgg atgaagatga
    46441 aaataccagt cggcgtccgg tccaactgca ttctcgcata gatatgaagc cgcgccgctc
    46501 cagatagtga tcttctggta ccttttcatt actgggatct gcgcaagatg gactgaagat
    46561 gccatacctc caacgaagaa atcaacctta tctactgtag caagtttctc aattgctgca
    46621 gctccctttt cgggcttcag ctcgctatcc actacaatta gctccagagg tctaccaagt
    46681 aatccccctg cagcgttgat ttcgtcgatg gcaagcttca ttgcttttgc agactggtca
    46741 cccgtgatat ctcccagggg tacgacggca ccaatcttga ttggagtggc ggcaaatgca
    46801 acaaaagcgg ttacagccac caccaataat actaacagta ctcttttcac tttcccacct
    46861 cctacgtagg ttgttcatga cgatttttgg tgcactagta catctagact tcattacaga
    46921 aacgcagtat ttacgatttg caagagatta gactctcttt actactactg cggttcccat
    46981 tccgccgcca atacacagag aagcaagacc aaactcgaga tttctcttct ccatttcata
    47041 caggagagta actattatcc tgtttcccga tgcgcctatt ggatgaccaa gagcaatcgc
    47101 acctccattg acattagttc tctcaagaag ccattccttt gaaacaccgt agatttccat
    47161 gagacccttc agtactgaca aagactgaga agcgaatgcc tcgttaagct ctatcaactc
    47221 catatccttt atgttcattg agaccttttt gagtgccttt tcgaccgctg gaacgggacc
    47281 gaatcccata taggcggggt caacaccggc ctgcgcaaaa gagactatct ccgcgatcgg
    47341 tttcagtccg tacttttcta ctgcttcttc actcgcaagg actgtcgcac ttgccccatc
    47401 gtttattccc gaagaattcc cggctgttac cgaaccatct ttcttgaagg caggcctgag
    47461 tttgccaagg atttccatgc tggtatctct tgggtgctca tcggtatcga aaacgacgtc
    47521 tccctttctt ccctttatca caaatgggac gatttcatct ttgaatcttc ctgtcgcgat
    47581 cgcttccttg gctctcttct ggctttcaag tgcaaattcg tcctgttcaa gcctactgat
    47641 accgtatttc tcggccagat tttcagcagt tactcccatg tggtagtcat tgaaaacgtc
    47701 cgtgaggcca tcccgtatca tatgatcaac tacagtaccg ctgcccattc tatatccgaa
    47761 ccttgcttta tctagcatgt atggggcaat agacatattc tccataccac cggttagcac
    47821 tagctcggct tcgcctgtga gaatatcttg cgcccctatc atcgtggact tcattcctga
    47881 accacaaagc atattgactg agtatccaac gctctcaatt ggaacacctg caccaatcga
    47941 tacctgtctt cccggtccca taccctggtt tgcagttaga atacaaccca ttattgtctc
    48001 ttgaatgttc ttaggatcga ccttcgactg ctctattgca gcttttgagg cagtcactcc
    48061 aagatccacg gccggcacat cctttagagt tcctccaaaa gtaccgattg cagttctctt
    48121 agcaccaaca atgaagacct ttttcatcga gaaacctcct atattaatcc aatgtcgcga
    48181 gcttgttttc ttagctctga tctgaagtcc ggatgtgaaa cattcagcaa ggcctctact
    48241 ctctggaata cgttgagacc tttaagcctg gcaatcccaa actcggtcac aacataatca
    48301 gtatcctgtc ttggaacagt aacgggtgag ccaagcggaa gaagaggaac gattgtagaa
    48361 acggtgcctt tctttgctgt cgatctaagt gcgatgattc cctttccatt cttagccatg
    48421 gaggcacctc tatgagtgtc tagttgtcct ccggtaccgc tatagtgccg agttccaatt
    48481 gcttccgaac atacctgccc cgtgagatcg acagagattg ctgtatttat ggagaccatg
    48541 ttttcgttct gcgcgacaac gtacggatcg ttgacatagc tgcctctgag cagaagcaca
    48601 ccagggttgt cctcaatgaa agaatacatt tcctttgtgc cgagtgcaaa agtcgcaata
    48661 aacttcccgg gccaaagact cttcttccta tttgttatcg ctcccttttc gaaaaggtga
    48721 atcatcgact cagtaaacat ttcggtgtga attcctaagt cgtgcttatc ctccataagt
    48781 cttgcgacgg cgttcggaat ccctccgatt ccaatctgca aagttgctcc atctacaaca
    48841 agcgaagaaa tatgtcctgc gattctcatt tcaatctccg agggttcagt gtccggcagt
    48901 tcaggaatgt caaaagagtt ttctatcaca taatcgacct cacttatgtg aacgtgagtg
    48961 tctccatgag tcttcggcgc tttctcattt acttccagaa ttaccatctt tgcgttctct
    49021 accatatctc tttcatatac cgtggacaat gaaacggtca tgtatccatt tctgtccatc
    49081 ggtgaggcaa caccccagaa tatatcgggt ttgtttgcca taatacgctc aatgccagcc
    49141 gtatgaagat tgtttgggat gtaagtcact gtcctaaggc cgctgctgac tgccttccta
    49201 gcacccaccg aatgaaacca agactcgttc agaaatctgc cttcatattc tttattaacg
    49261 aagaacggat actctccaat attcaaacaa gtagcaacgg ccacattctc tacatcctca
    49321 actctgtgga ggttctttag aagaccctga gcttccattg ccgccatgct tgtcacaatc
    49381 tttgttccgg acttaatgtt tctcaaacct tcatcaatcg atatcttctt ctttctgtat
    49441 tcatcattcc agttcaacta aaagcacctc cataaccttt cgccataaga gaagccagtt
    49501 ccagcaaata ctcgttccat ttttcgtggt ctttcttcaa tttgaaaaga agatcaagcc
    49561 ccatgaagtg tccaatgccc atcagaaaga cgccgagatc acgtgagtct atcgatctaa
    49621 tctcgtcttt caatctagat tcttcaagag ctcttatgta tgaagttaag agcctttcat
    49681 agtagaaacg tccagtctca ggttgaacaa attccgactc acggactatg ttatatagct
    49741 ccttgtgaac atccatgaag agtaaaaacg ccttaaagga cctaatctct ttgtttaacc
    49801 tgccttcaac accggagacc gcatctctca tcgttcttct cagattcctg ttcgcctgat
    49861 aaacaagctc ttgaagtaac ttccccttgc tttcaaaatg aagatagaac gtaccctgac
    49921 cgtagcccgc cttactcgtg atatcatata tcgatgtacc gaaatatccc ttctccccca
    49981 ttagtttctc cgccgccata aggatgacct ctttagcctt tccttcaccg tctgctctta
    50041 cttcatctag agtgaaatca agagaccttc gatccaactc atcagactta acaaaaccat
    50101 tgagggcgaa gtcgacgaga gatcgcacag tcgaatctgg cacccttgtg tcgttgtata
    50161 tgatccagta aatggcagca aacctggaac ttccaatgag aaaccacagt tcaggcattg
    50221 aaagtggaga cctgtcgttt atcacagttg agagaagagc gtccttgtag gttttgtcaa
    50281 cggagttttc cacatctgga aacctgtatt cagcctcatg aaaggcaata aactttctcc
    50341 gcgattccca aagaatatca aagacctttc tgtaaaggtt atacagcttc tcctctatag
    50401 tgtttcccat gaccgaagca gaggcggact cgaacctggt cttgatatcc tggacgattt
    50461 ccttgaatac ctgttcctta ccagtgtaat gtctatagaa tgctccattc gacagtccgc
    50521 aggtcctgca aatgttcgca acagaggtca tctcgaaacc aaggcggctg aacagctctt
    50581 cagccgcctc tttaatctgt agtcttgttc tctcgcttcg agagaccttc aaattacgag
    50641 acccccgtca acgccaatta cctgaccggt caaataactc gactcatcgg aaatcaagaa
    50701 gaagtagaca ttcgcaatct cttcggggag tcccattctc ttaagaggga tcttctcttc
    50761 aagtgctacc aatatcttct caggcatttt ctcagtcata ggcgtcttta tgaaacccgg
    50821 tgccacggcg tttaccctga tctttgcgcc ctttcttgtc aactctttag cccatgtctt
    50881 agtcattgca ataactcctc cctttgaagc ggagtagtta gactgaccga tatttccgta
    50941 cacgccgaca atcgatgaag tgttgacgat cgaaccataa ccctgatcta tcatcactgg
    51001 agcaaccgcc tgtgtcatgt tgaaaacacc tttcaaattg acgttgataa ccaggtccca
    51061 atcttcttca gacattctct gaatcagagc gtctctagtc actccagcat tattgataag
    51121 cccatcgatg cggccgaatt cgctcttgat ttcacctaca agttcagtta ttgcaggcct
    51181 atccgtcacg ttgagtctct tcggaattac cttgcccgga agctcggaaa attccttctt
    51241 cagattatcc aaaccttcga cgtccatatc gcaggcatag acaatcgctc cttcacgggc
    51301 aaacttttcg actgccgctt tacccagtcc actcgcgcca ccagttatga tgacgacttt
    51361 accttccaat ctcacctttt cgcctcctaa ttcttgcccc attttactgt cgtcgctgcc
    51421 cagacgaagc ccaatccggc accaaccatt acaaccacgt caccatcttt aatctttccc
    51481 tccctcaaac ctaattctat cgagagaatc tgatcattct gcccaagatg gccgtagtaa
    51541 tcaaggtaag tactctgatt ttccttcagc ccgagctctt caagaactgc aaaatgagcc
    51601 gatcttttga aatgcaaaat agctaaataa tcgatatcac tttcggagaa gccgctcttt
    51661 ctgagtgact ctctaatcac ccagtagaaa ttaggcattg tagtctctcc gaggcgtttc
    51721 ttgaactctg cagtatctcc ctggacggag aaatgatagt tctgcgtatc ttcgggggtc
    51781 ataggccact tctttgtccc cccgaccggg acaacacata actctgaaaa tgatccgtca
    51841 cccttaaagg ccgaagatag gacgacgttt tcattatggc ctttcttaag aactacagcg
    51901 gccccgctgg ctcctacatc aagcatgaac gatgttgatg gttcagaaag atccataaga
    51961 tcaacatttc tatagccgct tactagcaga accgtattga catcgtctct agcaatcatg
    52021 agactctttg ccacgtcaat gccggccatc atagatccac acatggcttc catatcgaaa
    52081 gaccaggcgt ttacagcacc gatttcgtca gcaaccttca gtccggccag ccagcaaggg
    52141 taatctttat gctgagcacc gttccagatt aacagatcaa tgtctttggg atctatcccg
    52201 gcattctcaa tagctttccg tgccgctttt attcccaaat tacttgtggt ttcaccggga
    52261 cctggaacga gtcttctctt cactccaaat ttcagttcga ttacctcttg cggaataccc
    52321 gttgaatcgg caatttcctc tggagtcatg aaggtctcag gtatgtatgt cccgattccc
    52381 gctattccaa ttattggaac tgaactcacc aagatcaccc cttatggctt gcgtgtcatg
    52441 attcatctct cacatatgaa agatgaatca tctctcactt tcacaataca ctaaaccgat
    52501 ccggttgcta aactcaatta aagtagttta ttgatgatta tcgacgaaag aagatgaata
    52561 actaccaaat tgtccttttt tctaacaatt cttaagtatg aacccgatga ttgctgtcat
    52621 gaattctcct ggtttttcga ggaaagctcc gtgaccggca tcttttatga caagaaattc
    52681 cgaatttctg actctttcat gaattgcttt catctcttct atcggtgtaa tgatgtctcg
    52741 atcggcacca agtagcagag tcggacactg aatcttgtca agttcttgca aaacattgaa
    52801 atcgggattt gaagaaataa gccttatgaa cccttcaaac cattctctgt tgagtgtcga
    52861 tttgaacatc ttctgtcttt cttttagcca ctgcagatta cgattgtaga agaaatctga
    52921 ataaatgaag ggtattgcca gcttgaagaa ccgctctcca tctttaagtt ctgcagcttc
    52981 tttccacgct tcgccaagtt ctgcaaggta tggagaaatt cgggctggag tattggcgag
    53041 tactagagaa tttactctgt ccggtctctt tattgctagc atttctgcca cctggcctcc
    53101 ataggagaga ccaactacgt ttgcgctaga aatctcaaga gcatcaatca ggtcggttag
    53161 gtcgtcaacg tggatggaga tgtgatactg tgaatccttt ctctcagatc ggccctgatc
    53221 tctgaaatcc atcagaatca atctgaagtg ccttgaaaag tggggaacaa atcctgccca
    53281 cgaggggacc gacatcataa ttccgttgag aatgataaca ggtgggttag attgatctcc
    53341 gtgtacttcg taatagattt gagttccgtc gctagttctg agcagtgcca taaattcacc
    53401 tccatcctta ttattgcatc gatttcactg attagtcaaa accaatcttt gatgatagac
    53461 tttaaagtga atggcacccc tggaccgata gatcatcgaa tttgtctcac gaaaggatga
    53521 ttgatttgtc aaaatttctg gaagatatgc ttgaacaatc agaagcagta agaagcatta
    53581 tttccgcatc tgatgtcgtt tttgagaaag cgaagtctct aaactttgaa agagtcctgt
    53641 tcacaggcat gggagcttct cttcatgctt cagaggtagc ggcttcattt ctaagatact
    53701 ctggtatcga cgcagaaagc ttcgagatgt cggagatcat aacttactca tcccaagaac
    53761 tgctaaaaag atactctaca atctttctca tcagtcagtc aggagaaagc gcagaactaa
    53821 tccgattcat cgaagaaaac aaagcgcttc tgccttctat gacacttgta accaacaacg
    53881 aaaaaagcag tgcggcaaag gctttccctg atgaaagagt atttccgctt ttcgcgggca
    53941 atgagagatc tatgggtgca acaaagacct tcgtgaacag cattgtctta actctgatga
    54001 tttcagcctt caaaaccgca agaagattga gcttttctga cctgcccacg agaatggatg
    54061 aagctttgtc gcatgattta tcgtggcttg cagctttgtt atctgagaaa agggagagga
    54121 ttctcgttgc caggggtttt ggcataggtg ttggaaagat ggccaggcta atgtttgctg
    54181 aagttgccaa gcttagtctc atattctatt cgggagcctc tttcagacac ggtcccgtcg
    54241 acttgcttgc agacaagccc gttgtaatca ccatgaatcc caagggagag acttacgacc
    54301 taatgcagga actccacaat gatatttacg acaaatgcga gttgattacc ctgacgaatt
    54361 cgagagaggg gaatgtgaaa tcaatatacg tttccgatgg cctggaagaa gtccttgctt
    54421 tcattcccat gatgaatctt cttcagaaaa ctgcggagga aatcgccagg aataggggat
    54481 tcgatcccgg ctcaggtgta ttcggaacaa aggtgaccac caaagaataa tcaaaatgct
    54541 ttgaaagcaa aaacaacttc tttggaagtg gccttgaaga tttacgacct tactgaagct
    54601 tgggattgct taacagaaaa aacttatacc ggttcttccg tttggttgat acctgtaagg
    54661 ttctttctac gaactgcagc tgcccttata tctgacgcct ataataacct gcatggcttc
    54721 aaccaaaatc ctcacaaaaa ggttgtgctt atcaagccca ccaacggatg aatctgaaca
    54781 acttcaattt cgttcgcacc tgaatgctgg aaaacttctt tcataaatga aatgctcgcc
    54841 gggtgatcta attcgttgtc gtcgaaataa acttcgtccc acgcatccct gtgctttcca
    54901 ggatcgtcga accacataat atcaccaaca acgatcctac cgggcctctt taaaatctgc
    54961 gtaagcttcg aaatcgcttc catcttcatc ggatctgtaa gatggtgaag agagtagatg
    55021 gctacaacct tgtttatgtt cattcttgat aggttcactt cttcgcacgg gttctcgaag
    55081 gaacccctaa agaattcgac gttctcaaca cccgcgctgg caatatttga tcttgcatct
    55141 tctaacatcc ttctgctaat gtcaatgcct atcacctttc tgcagtaagg actggcggcc
    55201 acggcccacc ttcctctgcc ggtcccgata tcaagtaatg tatctccggg gtcaagatca
    55261 agataatcaa gtgcgagcgc accaaccatt gcgatgatat cacctttgaa cggatcaata
    55321 tctgcaatcg gagaggcctc gggaagattg tcaaattctt gctcaagttt tccacgatcg
    55381 taatgctgct tcaaatgact cacctcgtaa ctctacctta tcatctagat tatactccag
    55441 gcaacagtgc gacatgactg aaatccgaat cagtcagcag atctgaatct cttggccttc
    55501 tctgaagctc ttgtaagcag catcaacgac tttgagagcc tcaagtccat cttctcctct
    55561 tgtgaaagga tccttccctg acctaagaac ctcaacaaaa tcctcaatca aaaggcaatc
    55621 aaagttgtca ccataagctt cccatcgact acccttttcg ttggaataga cctcgatctt
    55681 tgagttgaag acatccagga agagcgttcc ctcagttccg actatgtgaa gagttacgtc
    55741 tccccagtaa ggatgggcct tcggtctcga ccaactgcag tcaagtgtca tgtactggcc
    55801 agtcgagagt ctgaacatct ccagtccgca gtcttcgacc ggtatgtcat atataagggt
    55861 accgcaagtt gcgtacacct gtgtgaattc gcagtcaaga agccatctca ctatatccac
    55921 cacgtgcacg gtgtggtcca taacggcacc gccaccgccc agcaccgggt ctatgaacca
    55981 gccgcccggc attctcccgt ggtttgttcc caccagactc accacttcgc ccagcagacc
    56041 gcccgcgatg atctctctgg cctttcttac ggaggcactg tatctgactg ggaaagctat
    56101 ttgaagcttc acgttgttct cgcggcaaat cttaatcatc gcctccgcgt cttctacggt
    56161 cgtcgcgata ggcttttcgc atagaatgtg ttttccggct ccggcagcga tctcgacgaa
    56221 ctccctgtgg gtagagttct cgctcgttac tatcacaccg tcgctccgag cgacgagttc
    56281 ttctggcttt tcacagggct ttatgcccag gtcttcgcag gctttgttca ttctagatct
    56341 gtcctcatcg taaagcccca ctatctcgat atcattcatt ctcttcaagg ctctcacata
    56401 gctgtaaccg tgcatatgtg caatacctag gattgcgatt ttcgtcatct caaaacacct
    56461 ccgaaagctc gaccggtctg tgcagttttg cagacatgtt ggccgccagc gcgactctca
    56521 acgattctat ggcctcttgc ccgttaacgg ggacttccct gtctttctct actgagagta
    56581 tgaaagactt caattcttcg gtgtacggat caaaataagc cggattctcc tgcaagtatc
    56641 tcggctttcc atcgatcgaa gtttgcagac gaagcgtcgt ctctctctcg tagctgttgg
    56701 ttatcattcc cttagtgccg acaaattcgt agaacgttcc aaaattgacc ggcatcccgg
    56761 ccggttcggc ccagctgcct tcgatatgtg ccagggcgcc ggattcgaac ctcagaatcg
    56821 ccataccatg atcgaaagag tttttcttcc tctccgagct cagcgttacg gacctcgctt
    56881 cgatggtctt cacggggccc agcagttttc tgagaaagtc gaaatcgtgt atggagagat
    56941 ctacgaagac gccgccgct
//
LOCUS       DAOQYI010000011        51232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_735_length_51232_cov_80.080505, whole genome shotgun sequence.
ACCESSION   DAOQYI010000011 DAOQYI010000000
VERSION     DAOQYI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 51232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51232
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_735_length_51232_cov_80.080505"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..977
                     /locus_tag="PKL93_04945"
     CDS             264..977
                     /locus_tag="PKL93_04945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ70643.1"
                     /translation="MDIKCIIKERQGALAEWLNVLNECIICGSETREIYNRTFKVKYR
                     HCGNCEFISKDPADWISEEEELKIYNRHNNSIEDPRYVAFFKDFIDSAVVPFVSNGRR
                     GFDFGSGPSPVLATILERDYGFKMDIYDLYFSPNEVFKGKKYNLITSTEVFEHLAEPV
                     KYFKMLSAYLEDDGLLSVMTLFHPQDHQQFLDWYYMRDMSHISFYTVRTMQVISDLVG
                     LELLFTDKKRYTSFKLRKK"
     gene            complement(1232..2404)
                     /locus_tag="PKL93_04950"
     CDS             complement(1232..2404)
                     /locus_tag="PKL93_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNQ70644.1"
                     /translation="MKYDFDRVIERRGTNCIKWDQADLFFGRNDLLPMWVADMDFESP
                     PEVVEAIVSRAKHGIYGYTARSKAYYESIVNWLYRRHGWKIKEEWIAHAPGVVAAVHI
                     AIMALSHPGDRVVVQTPVYYPFFKAIKETGRQLILNPLKESDGRYSMDFEDLERKVDS
                     RTKIIILCSPHNPVGRVWRRDELSRLGEICLNRNIKIISDEIHSDLVFGNNKHIPTAM
                     ISDEVSSITLTCVAPSKTFNLAGLSSAAVISSSSELLGEYSNMMSTVGAGMSNVFGTV
                     ALETAYNNGERWLEQLLEYINGNFVYMRDFLKTNFPELRVAELEGTYLAWIDFRNTGM
                     SVEELRSFLYEKAKVGFEDGSIFGEEGEGFMRVNLACPRAIVEEAMKRLITARKSD"
     gene            complement(2484..3932)
                     /locus_tag="PKL93_04955"
     CDS             complement(2484..3932)
                     /locus_tag="PKL93_04955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ70645.1"
                     /translation="MRVAFINPQGNFDREDSYWTTHPDFGGQLVYVKEVASAMSEMGI
                     RCDIITRRIIDERWPEFADEFDYYPGKENLRIVRIPFGPDEFLRKEGLWPYLGEFSRR
                     IIEFYRAERTMPNFLTTHYGDGGLTGAMLFKETGIPYSFTAHSLGAQKLDKLLQTGEN
                     RMKIEEEFNFSYRIAAERISMKYSAVNFVSTSIERFQQYSHPLYREFSDVGNDSKYSI
                     VPPGVNTDIFTANPSELDEAIEDRYKEAVERFSNASRFRLPMIVVSSRFEGKKNHIGI
                     VRAFANDRELHESSNLVIVTRGIKNPYEEFDSLEEPDRSVLKEIIDHIRRNGIEYGVI
                     FLNIENQHELSALYRISTKRKSIFALTSLYEPFGLAPIEAMACGLPVVATSNGGPAES
                     LREDNIEYGVLVDPLETNDIVRGLKKALFSSPSFWEELSSRGVDRVTEKYTWRSSAEN
                     YLNVIKEKIKGEYPEPEIPEYLYSGDGLPFIE"
     gene            complement(3929..4864)
                     /locus_tag="PKL93_04960"
     CDS             complement(3929..4864)
                     /locus_tag="PKL93_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNQ70646.1"
                     /translation="MSRIAIVGELLIDLISTNTVKDLGKATSFRRFFAGSPGNLASNL
                     NDLGIESILLSRVGNDPFGRAYLEHLRLKGIDTSFVQQDQEAHTSVVFVSKSQSSPQF
                     SAFRGADCLLEEPYDIDSFMKDTDFIHFTAWPLSCERSREVCCKLVDYALRTGVRISF
                     DPNYRQILWTHNQDGSEFIKGFLENVFLVKPSEDDANEIFGPGKPLDYIRKFHESGAK
                     NVILTLGQKGSMISDGNRVVTLPSCARRVVDTTGAGDAFWSGVLFGLLGGKNIFESAV
                     YGNYCAGFRIEHEGKEVILPSIDALKAIFEAGDNL"
     gene            5031..6737
                     /locus_tag="PKL93_04965"
     CDS             5031..6737
                     /locus_tag="PKL93_04965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNQ70647.1"
                     /translation="MEEISLKRLQRRYVGSDIDLSLWENLESELKRLEEYEIVDRDSL
                     ENFLLHWSELFMILQEKLAWKYIDMTRFADNEKKRLDYSKFFEDVYSKAEPYRIRLMK
                     KYYDSIHRRELDPFRYENFDAIVSNAVELFNEDNLSLEVQEKKMASEYAATIGSITAE
                     FRGKEYTLSQLSRFQLEPDRLTREEAWRLSMSKLDEVHESLENLFDRLLSVRVPQAAN
                     SGFTSYRDYMHQKKNRFVYSVEDIHRFHESVEKVVVPFVSELNEKRRKELGLDRLRPW
                     DISVEPSGRVLKPFETMEEFIQKAIRVLQKVDPEFGANLLKMKKNGLLDLENRKGKAP
                     GGYNYPLDETGAPFIFMNATGTPANVKTILHESGHAMHSFATTDEKLIAYRHATHEAA
                     ELASMSMELLTMDYWDEYYSEEEVRIARREELTGTLSFLPWCMIVDSFQQWIYTNPDH
                     SSSERDEKFSLVFDRFNTGVDWTGLEEEKKIRWLLQPHIFTNPFYYIEYGIAQLGALA
                     VYRNYRKNGSKAIEDYKKFLSMGYSRPLDELFESAGIKFDFSEHYLLELVRFVKSELA
                     LL"
     gene            6825..8615
                     /locus_tag="PKL93_04970"
     CDS             6825..8615
                     /locus_tag="PKL93_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70648.1"
                     /translation="MRKIVFVIVLLMIPFVLLGQVEELKAKIAENPLDVESLKALLEI
                     YYENYELESYGTTLKEVIMNVDEIPEAMYPLIEEGIEKLLENAYSDYAEEISEILFDL
                     RPDKISLLLYLKSLNSGWAMSEEAIRQSVNRLSEDQIEIYEFLHEESVDLYPVDLHLL
                     VSRILVEDFGETDYLIPYIVGLIDDYDFSTARSLLSENSEFLSSSPMYYYLNGILDLE
                     TGYYDEAVEWFLQGKSRDDGTEIYEALSLIEYIYIPEYVTDLFTAIVLANDEASLTSN
                     IQEYVLNYEELSWIFSWISRVPEPKKLAEILSDEETAVSYNTVNFDFEKINIGFYDAY
                     ASLLGTVSSAIYGQFLDARTYVYISEKMDGVIVEGEREARFDGYDVYSLSPKRDRLLI
                     MDTWGESISMVDSSMDILWTLSEEYSMAPVAWSPDGSKVVVSTGGDYFSVIDVESGQV
                     IESIESFYDLVFLSLDETYAVDYDGKIVNLTTESVLDTDAPVYWAAYGNNDKIIYLEY
                     PDEYSDFSYDSLHVYDLSTGDDSVIAYNAMFMDAPVPSVKIEGDFVYFTEKDVQGMHR
                     VIVMDYTTNEVLFESLPSEDIIIFPDVRPE"
     gene            complement(8700..9602)
                     /locus_tag="PKL93_04975"
     CDS             complement(8700..9602)
                     /locus_tag="PKL93_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNQ70649.1"
                     /translation="MGFEVAVIGAIGIDTNVYLSGSEIDFSVEATFTEVVDCIGQAGG
                     YVSQSLKRLGHEVCFVGFVGSDYFGDHIRETLSAQGIDIAFLKTDPLGTKRSVNIMYK
                     NGERKNFYDGRGAMALKPDVEAIKKYLFGTKLAHVNIVNWSRFLLKPLREAGLVISVD
                     IQDVTDPDDLYREDYVREADVLFFSSTNFRDPTPIISKYLRVRNDRIVICGMGKRGCA
                     LGTEHGINFFGPVELDEPVVDTNGAGDSLAAGFLSSYFLEGYTLEESILRGQIAARYC
                     CSKKGVSSDLITKEQLDKTFAGKK"
     gene            complement(10028..10189)
                     /locus_tag="PKL93_04980"
     CDS             complement(10028..10189)
                     /locus_tag="PKL93_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70650.1"
                     /translation="MRRFDVCIIKVGGQCIGLLSEILLRTVDHSGQWAIGTDTYGLAQ
                     RGGVWFYLS"
     gene            complement(10218..10448)
                     /locus_tag="PKL93_04985"
     CDS             complement(10218..10448)
                     /locus_tag="PKL93_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70651.1"
                     /translation="MIIVKHSCMLEFTRELKRSHKPSPNPVKTGMKCNRNYVSLTLPV
                     RVIKLPKTVQFGFNTISVSRIDPEFKLSLRVK"
     gene            complement(10847..10984)
                     /locus_tag="PKL93_04990"
     CDS             complement(10847..10984)
                     /locus_tag="PKL93_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70652.1"
                     /translation="MGITVHAKVDLKDGFSGSGITDSGYKVRIGKEGSAPYDLLLMSL
                     A"
     gene            11222..12199
                     /locus_tag="PKL93_04995"
     CDS             11222..12199
                     /locus_tag="PKL93_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HNQ70653.1"
                     /translation="MNLYALFNSSLYHGTSSRKGFFEGWYFKFVDRKKEKSFAVIPGV
                     SLNSSREDAHAFVQFLSGGKNESGNFNYPLKEFHSDKKHFSVEISKNYFSAGAVILEM
                     CNENHRIAGNIRILEPYLWPRKFYSPGIMGPFSFVPFMECYHGVVSMDHKLEGEVRID
                     DELIDLSGGRGYIEKDWGRSFPEAWVWMQSNSFDLERTSFMLSIATIPWLGRSFTGCL
                     CAFLFKEKLYKFTTYRGAKIDRVEVLDNEVFLQLRQAGFSLNVSAKKTSGARLISPVE
                     GSMSGKIDESLTSEITVRLLRNDAVLFEGTGTNSGLEVVGEFELKEKSK"
     gene            complement(12363..13193)
                     /locus_tag="PKL93_05000"
     CDS             complement(12363..13193)
                     /locus_tag="PKL93_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70654.1"
                     /translation="MSSQALARELRASWAFIERNFNFTKRYWKWELVFFVYTMANSVT
                     MGFIGKGIEAFSGTKLDTSYLILYMLLGSILWGYLSILFEIVAETVAWERWEETIEYT
                     FMAPVRRATHLLSTCGYAILYGILRSGLILVVVSVFFDLDLGRANFLSAAGILAVSSF
                     SFVGLGIVAAVLPLISPEKGVQVVHIFQALLLMFSGVYYEIDVLPMWMQQVGKLSPAT
                     YALRGMRASILEGRTMPEMWNDVWPLILLGALLLPFGLVFFKRMEVWAKKRGVLKRSG
                     "
     gene            complement(13190..13984)
                     /locus_tag="PKL93_05005"
     CDS             complement(13190..13984)
                     /locus_tag="PKL93_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70655.1"
                     /translation="MADALLANNIVKAFKKSRKEIKALKGISLRIKEGEIFGLLGPNG
                     SGKSTFIRIASTLLIPDRGSISIFGYDSVSNSASVQRMINRVSVEASFFRKLSAVENL
                     CFSAGLYGIPRREALKKINGLAERFGLDLKRLKDPLEDFSRGMQQKVAIVRAFMTEPK
                     MLLLDEPTTGLDPRAKRDVQSLISDVKNTMNTTMLLTTHDMDEAEKLCDYVAIIHLGR
                     IVASGRPSELKKQLLGKVDNPSFEDVFLEFTGMSIEEAEYQEVESA"
     gene            complement(14827..16674)
                     /locus_tag="PKL93_05010"
     CDS             complement(14827..16674)
                     /locus_tag="PKL93_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNQ70656.1"
                     /translation="MKRFLLVALLLLSTVASVGGNLNLYFGNPHSHTSYSDGSGVPEE
                     AYSYAKEVPDLDFLAITDHAYYFAQDLPDGRNKLAATIEAAIEATSEDFLAFVGFEWT
                     ATGTGHINVYGTSDWTDRVKSDLWQLYDWIIEHDAVGQFNHPVRDFGDNFKEFQYSPE
                     ADLYMNLIEVGNGSWWENDTIVEEMFSAYREALRKGWHLGTTLGQDNHKPNWGGANDS
                     RTAVFATDLSEKSILEAFKKRRTYATEDRNIEIHFSTEEAFMGDIVRDADRVELRILI
                     RDTADDPLEIVEIYSNDGVIANFLVDSHEFYIEMTTNIEVGFQYFFVYARGHDGEEAV
                     SSPIWVERSCPIHLYNSAAFPESVKPGERVTLSFQISNLSSEEASSLLQIENLGGIIH
                     EEIVTLEGFESKIVHLEKTAEEHDARILFLLDSIPYSSTWLEIRSSASLNVLVDRSHI
                     NYASDEREKLKMLLEEMGNRVSTADRIFKVGELDTIDVLLLPLPGAGGSFDRLKILMP
                     QQSNIIKEFVMNGGRLIITGTGDEISNEIISSYNRLLEEMGIYATYEKAITSDAVELD
                     GIVFDGLSVLRGESGRHVYGKGEILLFPGDPFTDDVIENNKELLDQSFR"
     gene            complement(16768..17001)
                     /locus_tag="PKL93_05015"
     CDS             complement(16768..17001)
                     /locus_tag="PKL93_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70657.1"
                     /translation="MHIVLSHHGELQFGSPVTPKTREAILIWLCDNIDSRLDNFESHA
                     FSASKESRWTDFSKMLQSRLYLGKIENEEEIGD"
     gene            complement(17058..17804)
                     /locus_tag="PKL93_05020"
     CDS             complement(17058..17804)
                     /locus_tag="PKL93_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNQ70658.1"
                     /translation="MIDGKSGSYPVLSEILREYNSALDRVENYVNSKQGFFFIFSAKR
                     IEGSRGPYYDCLVGDSKCRTEAKAWISEQGCLEPVEGWIGLADYLIDDRFGLSIKIRK
                     LFSISEMESYSSGSITQLLPIVEDLKKIKSEVLELVESIKDKYLNTLVKRMVSDDGAC
                     PDFFEAPAAKVYHHARIGGLAEHSLSVVRYALALREASNSRPNINRDLIIAGGFLHDI
                     GKTRTYITESFKFDYSDDGYLEEHKLLAPG"
     gene            complement(17877..18008)
                     /locus_tag="PKL93_05025"
     CDS             complement(17877..18008)
                     /locus_tag="PKL93_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70659.1"
                     /translation="MNEAVSKSIMIFVEGYPTMFLATVAFFIMIKLLMKSASRKDSG"
     gene            complement(18022..19119)
                     /locus_tag="PKL93_05030"
     CDS             complement(18022..19119)
                     /locus_tag="PKL93_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNQ70660.1"
                     /translation="MNLLSNFSIPVGNLVMFAIGAILIYLAIKKEYEPMLLLPIGFGA
                     ILTNIPGSSAIGEHGVLTILYEAGIANELFPALIFIGIGAMIDFGPLLQKPVMFFYGA
                     AAQLGIFLTIIVAALLGFDIREAISIGIIGTADGPTSIYVASRLAIHMLGPISVAAYS
                     YMALVPVVQPPIIKLLTTKEERRIRMDNRTEKVPKYVRILFPILVTLLAGIIAPVSVA
                     LIGMLMFGNLIRESGVLNKLSNAAQNELSNIITLFLGIAIGSTMTAEAFLNSRTLLIL
                     LMGLIAFVLDTMGGIVFAKILNVFRKQKINPMIGAAGISAFPMSARVVHRMGLEEDPN
                     NFLIMYATGANVAGQIGSVLAGSIVLALFLG"
     gene            19344..20675
                     /locus_tag="PKL93_05035"
     CDS             19344..20675
                     /locus_tag="PKL93_05035"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNQ70661.1"
                     /translation="MRAVFPEGFLWGVATASYQIEGADFEDGKGPSIWTDFSHRPGRV
                     DSGDNGDVACDHYHRFSEDIELMKQLGINSYRFSISWSRVLPEGRGKINRKGSDFYNK
                     LIDRLLEVGIQPMVTLYHWDLPLELHKKIGGWESRDMRHYFGDYSSLVFSEFGDRVKH
                     WITLNEPYCSSHVSYLWGEHAPGKRDLKTSLIVAHNLLLSHGEAVRRFREVVKDGTIG
                     LANVSTFVEPATDSKEDRWAARIRDQFINGWFFETPITGEYPSELFKRFNDAGVQPLI
                     EDGDMDLISTPFDFWGVNYYTRNVVRKKESSILGSEVIQGELAKTEMGWEVYPEGLEA
                     FLYKTFKEYGKKPIYITENGMACKDKLTDGFVEDFERVDYMKRHFSSALSALKAGVDL
                     RGFYVWSLLDNFEWSYGFSKRFGLVYVDYEKGLKRIPKRSYYYYRDFLERD"
     gene            20809..21669
                     /locus_tag="PKL93_05040"
     CDS             20809..21669
                     /locus_tag="PKL93_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNQ70662.1"
                     /translation="MIGVVVDSGCDLPDEVKANDNVREVPLKIVLDGKEYRDNIDIST
                     SDLLEYMENKFPKTSLPRPIDIGEAFESLYEKGYNEILFIGISSGLSGTLDQVKSYVR
                     DFSEKHGDSKIEVVDSKNISIGSGLLAMKAIELIKSGESFEGVVKDILGSVRKAKVFF
                     CIPVLKYLRAGGRIGKVTATIGDILDLKPVITVGEDGVYHSVSKERGMKRAVKSSVEK
                     LIHFIDGNRVLNIAAYTSDKSEKTMEFFRMAVSEIKAAGFSHIITGQISQSLICHTGP
                     GLIGVAAILE"
     gene            22048..23394
                     /locus_tag="PKL93_05045"
     CDS             22048..23394
                     /locus_tag="PKL93_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ70663.1"
                     /translation="MYLLDRNSQTRILSVFEGAIYNGFFLITQGFLGTGLALEFGASE
                     PVIALIGVLPSVSQLIQLIAPFILRLVGNRKKVMMICASAGRLSTAFIPVTLALGINR
                     QSLLLIILAFFSFAASLAGNFWVSIIRDVVPPDKSARFFSLRNVIFTITNMLITLLYS
                     FILDHSSGKTGFVIITTMGAVSALVSLVLLGLHNDPPHEISYGSGLFKAVTRDKKFMT
                     YLRFYSFWSFSIAIASPFFAYHELVNMELDYSFLSLLNIFSSTLTMMFYLLWGRIADR
                     IGGQAVLEFGIFGAFLKTFLWLYMDRGTVYLLYIDTFIGTASWSAINLCLFTTMLELL
                     NGVDAETYYALLSFSNGAFALAGSLVGGVLASYLNKFTWTLGGHTFFGIQILFLVTLV
                     LRGVSFLLLKRVKTKKVVSVPGMVFNSAVVIANRLAARPREFIEVMIEKNKSKKKE"
     gene            23400..24641
                     /gene="pepT"
                     /locus_tag="PKL93_05050"
     CDS             23400..24641
                     /gene="pepT"
                     /locus_tag="PKL93_05050"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNQ70664.1"
                     /translation="MIEKLIERFLRYISIETTSSFESKTFPSTTGQLVLARTLKEDME
                     SMGLSEVTIDDFGYVMGTLPSNLERKLPVIGFISHMDTSPEMSGKDVRPSIVKYQGGK
                     LLLNKKDNLYIDPQLYPEIERYVGEDLIVTDGTTLLGADDKAGIAEILTAMEFIISNP
                     RIKHGKIRVAFTPDEEIGRGTKHFSIEEFGADYAYTVDGGGIGELQYETFNAANTEVV
                     IKGLNIHPGSAKDRMKNSMLIACEFNAMLPSNEIPATTCGREGFFHLISVQGSVEETI
                     LKYIIRDHSREKFSRKKMMISEVAALLNFKYGEDTVTFCTEDSYYNMKEKIEEVYFVV
                     ETAKRAMESLSIEPKIEPVRGGTDGARLSFMGLPTPNLFTGGHNFHGRFEYIPIRSME
                     KSVQVITKIVELFSEEATFES"
     gene            24893..26545
                     /locus_tag="PKL93_05055"
     CDS             24893..26545
                     /locus_tag="PKL93_05055"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNQ70665.1"
                     /translation="MSTSSRFVRERIGRDNPLFSMSRVTIETAFYGNNVVPVVSPSEA
                     YKLAKNSAGTIVTDLPVYKPEKAGLESDSKVLLFNDGAVTGRCASARRIVGEPGVNET
                     EYGAKLREAVYNTRNRKMYHAQAYIGLDRDFMVKAHLLVPETHENLIYNWLLNFQPIN
                     EFYNEMYESSERCENEGDIFVFSDPEWHHPDHPLGLSFFDPEHNCAAILGMRYFGEFK
                     KGTLTLAWGCANRHGFAACHGGQKRYNLQDRKFVVGFFGLSGSGKSTLTHAKHDGKYD
                     VTVLHDDAFVISSSDGSSVALEPSYFDKTSDYPLSSEDNKFLLSVQNVGATQDDEGRI
                     VIVTEDIRNGNGRAIKSKIWSPNRVDKFDEPVDAIIWLMKDPSIPPVVKVNDPVLASV
                     MGATLATKRTSAERLAPGVDPNALVIEPYANPFRTYPLSDDFNRFIELFTHRGIKCYI
                     FNTGHFGEKKVPKELTLQILESIVENRAEFLPWDPFKELEIMFIEGFLPDLSDESYKK
                     LLKERLVDRYNFLRSREVERGGFDRLPQEAVESISSLIKMVE"
     gene            27000..28205
                     /locus_tag="PKL93_05060"
     CDS             27000..28205
                     /locus_tag="PKL93_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNQ70666.1"
                     /translation="MRVLVTGARMWYAINTIRFLARSGHEVFAADSSRLSGGLYSRYL
                     KGKFIYPSVGENSSAFVESVIEKIEELKIDVLCPTFEEGFVFSKYIGRLEGRVNVLVP
                     SYDHITLLHDKYSMTTYARSLGISVPKTVLLKDFSLEGCEFPVIIKPRNQRSAEGIFK
                     VNSKSEFEKRCRNLEEDKYLVQEWKEPYQICTTGLAHNGRLIGNVIYHNIREYPESGG
                     IGTCRVSIDCEKALGEVKKIVSDLNYSGFISMDFLHDRINGEYYLVDVNPRMSPGLLI
                     AYNSGVDMVKAYLELANGSEVSVARSPKTGYGTFTTAMEIGWFFSTLFKGKFKNLKGF
                     FKSRKNLKDDSWDVRDPIPFFVMLLSMLNTAVFGPLRGGQTKSFSLGATCDTESLGSE
                     EELIKKKAV"
     gene            complement(28511..28876)
                     /locus_tag="PKL93_05065"
     CDS             complement(28511..28876)
                     /locus_tag="PKL93_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70667.1"
                     /translation="MVFTITTPDDKLANKLEPEVPWHSRGIEAFKALSRAGIDVEIAL
                     MPILPFTEDSTEDILPILTRGKEAGTKFIYPSMRMTLRDRQREYYYLKLDEFFPGMSN
                     QYKRTYGNSYFCSSTNDNI"
     gene            29516..29950
                     /locus_tag="PKL93_05070"
     CDS             29516..29950
                     /locus_tag="PKL93_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70668.1"
                     /translation="MGFFEGFSKIVFVISYVLWAGGGLTVSLLMIPMKDPRTEPQVQG
                     YLHAFWQTTRIPAAALSFVVLGAAITMAIIVSASLLWSSIFSLSWFLTFLFWLMADRL
                     MKSGGSDPRSMVEVHKRSFHAYLVMADISIFLSMLSVAFMLL"
     gene            complement(29945..31762)
                     /locus_tag="PKL93_05075"
     CDS             complement(29945..31762)
                     /locus_tag="PKL93_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNQ70669.1"
                     /translation="MLITLSKVGHDYGQDFLFDEVSTSIDRKDKTILLGKNGSGKSTL
                     MRIISGDLLPSEGEVFLSTNVRIGYQIQNRIPNSELTLMEYYMQDKSSVLPDTEEYYS
                     FDRRVRSILVGLEFGEEDWERRLETFSGGELTRISLGKLFLVDYDLLLLDEPTNHLDL
                     ESTEWLVSFLKNYKGALLVVTHDRYLIRNVGNRFWELNGGSLWDFSGPYDRYQADREI
                     MLKSGLRTRDNLSKEIERLDSVAKRYRLWGQEKFIKQAINKEKQRDRLIEQLESIDLP
                     DEEIRGTKFRLPQPDRTGYSVLKIEKLSFGFGSKNLFSSATTEIHRRDKIGLLGPNGS
                     GKTTLLKIITGNLKGYSGEVTWGHNIRWGYLSQLTEDLNPSNDVLTEIWQLMRGQPDY
                     EVRKYMGRFGFPGDDVFKPVHTLSGGEKTKLALAKLILSRPNVLVMDEPTNNLDIWSI
                     ESLEQVLKEYEGCIILVSHDREFVQNICDRFLMIDRHKLKSVSTVEEYLSRNQSYTGS
                     VGNDDARVSFQERRRLSNKKKSIIEKLQQLDAEEEALSNSLEEIHARMGLYASDYERL
                     QEFQNTLNQAEERILEILEEREILEHDLEELSTMMGDQS"
     gene            31902..32486
                     /locus_tag="PKL93_05080"
     CDS             31902..32486
                     /locus_tag="PKL93_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70670.1"
                     /translation="MRLRLPFLIVLTGFLAVSYIWGFNFFRSPYQVFLQYERAMIDYA
                     NQIRLQQDSGLEAGLSEDEIVSLRKVAIVELEGDMRNLIFDFHKYWSIIEKYYLQYAQ
                     SSGIWFTDGLSGSIYTQDDPEYDTFLALYGLKDDPESYCLIFVPEVSEESAFREFRGA
                     RIYFLQKTIWGYKIEWSRSLIDLILDVDSSSVSV"
     gene            32619..33908
                     /locus_tag="PKL93_05085"
     CDS             32619..33908
                     /locus_tag="PKL93_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70671.1"
                     /translation="MFFKKINDRRRENRITKIERRIPKSREEEKGRFLYELWNLKREK
                     GDNEGAMKAALERLAFLNNPESFEDLISTFEKIDGSSKNSFAREIEKFACEFDRGLWE
                     EFISVFFEDQPEAAIKFALSCYRISSKALFVEIALGRLEKAFYNEEPGRLVRLREMYI
                     STISEVGEISRVDQLRLLERALESSKSLIQDEQLLSNLAVKYIDLMLSGEDLDGIAKK
                     LSGCFDRFVVYFKGNEVTLLDKLDLILPKIQEEEIKRALIEKLRILGQSISDKRYDSD
                     FSGESDYLEFLKKSIANYEFKARKRDSGEELLRKLACDYERIIDILESPEEKLLYIRK
                     LGALLRDRLEEPYKALEVFEEGLRLKADDISLWLVKGMTLEKIARNESDPVKASICWK
                     RTYDHYHAIAETFEEQEVKLTAIEKCATIQAERLTGV"
     gene            33973..34710
                     /locus_tag="PKL93_05090"
                     /pseudo
     CDS             33973..34710
                     /locus_tag="PKL93_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            34723..36420
                     /locus_tag="PKL93_05095"
     CDS             34723..36420
                     /locus_tag="PKL93_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNQ70672.1"
                     /translation="MSQRLDGKKVKSLIEMLSGYHRMRGSRDYKESMNSITDYLIRSG
                     LPENRFRVLKYPADGVTKTGNIVSTLAWEPVYGELWLENPERVFVTSTKVTKVSLVSG
                     SGSSKGWESLPLVVYDGKGDYSGKAVLASEDPVKVFQHAVVEGGARCLILCHMRKVFE
                     EIGRSLNDLPQMTNFLTIPHDRDSADRNAVAFSVTKEKYDLLFNYSKKGSAIVGFRAE
                     TNMSKGSFDVLQIDATGFNDGPQVLITAHLCHPSPGADDNGSGAALAVEIARVLNDEN
                     FPYSVKIALVPEYLGSVPYALQLKSENNLPIYTINLDMVGADQNKTGSTFILSKVPPY
                     LPQRWGNILEFYIKTLMPSNAGYPLKRFGEIPFMAGSDHCVFTTLGIPSPFMGHLPDR
                     YYHSDLDTPQMMDEKELEWVGLSALNTLDQLVKPDQDLLISVRSKMIGELFGILNRIS
                     GREGSDDVFDLLISNYEGEVLRKVFNNSGNLPSLSPLEPTFDSSLGLEWIRTFPQELK
                     DELEIDFASIADFVVGGAAVVGGREAVELLASIHYGVEIGKVRVLTGWMIEKGLLRS"
     gene            36510..37109
                     /locus_tag="PKL93_05100"
     CDS             36510..37109
                     /locus_tag="PKL93_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70673.1"
                     /translation="MPWSGFLEVSDSERTQLLMKNDFRAYFLPTVGYEISGEHLIELS
                     FSHFSVKSTFEATDTDPLSSYESMFKQDVLSLTYMYILPELDRFSLQLGVQGRLWFKQ
                     LTLTGSKTLDRKVSDLSFGPAADLRLQMNDKVFIGLEVSTGLGKGNGFIDMFAGLHFD
                     GPFYYVRAGTRYWSVTNSFENDRFFLSFVNLGVQFCAVF"
     gene            complement(37106..37711)
                     /locus_tag="PKL93_05105"
     CDS             complement(37106..37711)
                     /locus_tag="PKL93_05105"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNQ70674.1"
                     /translation="MDEVVLSRQEIFSSFEEAVRNKGSVLGNGILRVDSFLNHQIDPQ
                     LMKNAGELIADYFRTYGVTKVLTAESSGIAPALMAASSLGAQLIFARKSRPITMQRYL
                     SESAPSHTKGGVVELNVSLDYIDEKDRVIIVDDFLASGKTIEALARLVLKTGASLHGF
                     AAIIEKTFEEGRTLLAKFGVPVLGIVRISSLDPLSFVKNGV"
     gene            37985..38374
                     /locus_tag="PKL93_05110"
     CDS             37985..38374
                     /locus_tag="PKL93_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70675.1"
                     /translation="MNGYYRLGFEVFGASKSGSLSFTPTMLYVGPSVSYSGALGKVSG
                     LEAGIDARLLTGIENLSFNLFGRRFMFALGLRGQAMYSFFETTDNFKTRLSPEISFVD
                     VTKMTDRFKFSLFFWPLPALLGFDMYF"
     gene            complement(38403..38528)
                     /locus_tag="PKL93_05115"
     CDS             complement(38403..38528)
                     /locus_tag="PKL93_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HNQ70676.1"
                     /translation="MKVKNVGEVYRCEICGNIVEVKEAGGGELVCCGEPMKLVQK"
     gene            38600..39034
                     /locus_tag="PKL93_05120"
     CDS             38600..39034
                     /locus_tag="PKL93_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70677.1"
                     /translation="MERVFFAVLFTIFIVATAGTLYQEPQAIFASHLQDGTLVYGGSV
                     MLSWDVVVRNNSIILSNLDIWVSNRDSGARVVLESSPFIEIASYNLYLGTSPTPKLYA
                     STGSNSYFVDRLSNDTVYYWQIVAIDKNGNRYYGPIWYFETR"
     gene            complement(39035..39988)
                     /locus_tag="PKL93_05125"
     CDS             complement(39035..39988)
                     /locus_tag="PKL93_05125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNQ70678.1"
                     /translation="MDKIFFTYKIPDEGLLLLKGYDIMINTEDRFLSKEEIIQKAKDA
                     VALVTLLSDKIDAELIKSLPKLKVIANYAVGYNNIDVEEARKRGVRVTNTPDVLTDAT
                     ADLTLALILATSRRIVEGDRLVREHRFAGWKPDLLTGPSLKEKNLGIIGLGRIGRAVA
                     KRAQAFGMKVIYHNRKPLLTEEEEKLGVKHRSLEELLKESDFVSIHVPLTRETHHLLN
                     EKRLSMMKPGAILVNTARGSIVDEAALIKALKNGRLAAAGLDVYEEEPIVPQSLIDMD
                     NVVLLPHVGSATKEARTEMAIMVGRNVAAVLEGKEPPNAVV"
     gene            complement(40053..42437)
                     /locus_tag="PKL93_05130"
     CDS             complement(40053..42437)
                     /locus_tag="PKL93_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNQ70679.1"
                     /translation="MINYEEYTEKAKKILMDVQDILMRYKQNQLASEHILLSMLEDGD
                     NIAIDILNEKRVSIGSLKKDVEEVIGRYGSGSQIGNQIYITPDARHILENARSEARRM
                     HDSKTGTEHILLAMVRETSAVASRLLSKYGVNLDNIYTLILKGRTKGDAEEDENLSSL
                     KKFTIDLTQLAREGKLMPVIGREDEVRRVIQIIGRKTKNNPALVGEPGVGKTAIVEGL
                     AQRIVSGDVPEYLNNKSVLALDMGRLVAGTKFRGEFEERMKGIIDSVRKLSGEVILFI
                     DEIHSVVGAGSAEGSMDAANLLKPALARGELQCIGATTLDEYRKYIEKDRALERRFQP
                     VMVEQPTVEEAYRILEGLKPTYEKHHDIKIEDDALKTAVDLSVKYITDRYLPDKAIDL
                     VDEAASFVRLQTGYMPEKLRSLDRELRELDGEISAHAEKGEYRTAAELKTVAEKKRRE
                     FLLEKEKWESEESFGKTVTSAVVASIVEQWTGIPAGKMLQSEREKLRDLESIIHEKFV
                     DQEEAVKIVSQTIRRARAGLNAAHKPWGSFLFMGPTGVGKTELAKRLAFILFGSEEAM
                     VRIDMSEYMEKHTVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLLDEIEKAHREVH
                     SVLLQIMDDGRLTDGKGKTVSFSNTIIIMTSNIASEELASTSELSRAMDLAEESLRKA
                     FRPEFINRLDSIVLFRPLDSEAMEEIVKMRIAEMEKRLSDQHISVEISQKAVEYLAKE
                     GYDREYGARPVRRLIEKLVEGPIADLIIDGALGEGMKVIIDSDASGILVKAQEG"
     gene            complement(42494..42943)
                     /locus_tag="PKL93_05135"
     CDS             complement(42494..42943)
                     /locus_tag="PKL93_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNQ70680.1"
                     /translation="MLAIRRGSDLFRPFEEIQREMDRLFNDAFKGLSDQSRETTMFSP
                     EVDIYEKDNSVFIEMDIPGIKKDELEIKVEDDVLSIKGEKKLEREQKERDYHRYERYS
                     GAFQRIFRLPDYVKSDEVKAKYEDGVLKLELPKKEEVKKEAIQVKID"
     gene            complement(42981..44825)
                     /gene="dnaK"
                     /locus_tag="PKL93_05140"
     CDS             complement(42981..44825)
                     /gene="dnaK"
                     /locus_tag="PKL93_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNQ70681.1"
                     /translation="MANKEYTVGIDLGTTNSVISWVKPDGNVEVIPNAEGNRTTPSIV
                     SFSKTGEIIVGEPAKRQSILNAERTVRSIKRKMGSDFKVKIDDKEYTPQEISAYILKK
                     LKADGEAYLGGKITKAVITCPAYFNDAQRQATKEAGIIAGLEVQRIINEPTAAAVAYG
                     IDKKKGDKKIIVYDLGGGTFDVSVLDIGDGVVEVLSTSGNNHLGGDDFDQRLIDYIAE
                     DFRKKNNVDLRKDKQAFQRLKDAAERAKIELSSKYETEISLPFITATADGPLHLEMKI
                     TRSTLESLIKDLVEGTREQIERALNDAKLSPKEVDEVLLVGGSTRIPMVQSFIKSIFG
                     KDPNKNINPDEAVAIGAALQSGILSGSVESDLVLVDVTPLTLGVEVMGGLMEPIIERN
                     STVPVKKSKVFTTAADGQTEVEVAVFQGERTMARDNMSLGSFKLTGIAPAPRGVPQIE
                     VTFDIDSDGIVHVSAKDLGTNREQSMVVSGRQKMSEEDIKRIVEEAKKYEEQDKQKKQ
                     EVELKNQADQLAYSMEKLLNESGDKISSEDRGKLEGLVKDLRDALSEDNIQRIKLLFD
                     QLQKESMRVGQSVYQKAQAQAPADGGPQEGENHDSDTGTEYIPPEDSK"
     gene            complement(45135..46217)
                     /locus_tag="PKL93_05145"
     CDS             complement(45135..46217)
                     /locus_tag="PKL93_05145"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNQ70682.1"
                     /translation="MQKKKAVNISWKKSGILESFSDLLPELKDFNSLSLGEGLTPLTK
                     IEHAMTDGLELFIKNEAINPTWSFKDRGSYLAVLDAINKGYKHIGTVSTGNMAASVAA
                     YGMRAGLKTTILVSDSIPEEKISPIAIYGPRILKVKGDYDRLYFRSLELGRKKNIYFA
                     NSDIPMRVEGSKTIAFEIFLQLEGRVPDFVIVPTSSGGNIRGIEKGFRELKACGLSRS
                     IPRLIVAQALGCCPIHQAFSSNDNQIEKFTHAHTIAHAIENPFPPSGNAVLRMLKENQ
                     GITVAVNEDEILTAQRQLAKRGLFGQPASAVAFASISKLRKAIDVKRAIVVAVLTGSG
                     LKYPAVLKEQPSVAEVTDIDSLQDLL"
     gene            complement(46323..47105)
                     /locus_tag="PKL93_05150"
                     /pseudo
     CDS             complement(46323..47105)
                     /locus_tag="PKL93_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            47253..47480
                     /locus_tag="PKL93_05155"
     CDS             47253..47480
                     /locus_tag="PKL93_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70683.1"
                     /translation="MEIKGYVEIDGVTYKLISRQKSAHKKLRIRSKGQTGVHLLEEES
                     FLKENSDYLKSLLNDSEVAIPVGKNSGGQDE"
     gene            47473..48723
                     /locus_tag="PKL93_05160"
     CDS             47473..48723
                     /locus_tag="PKL93_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ70684.1"
                     /translation="MNEFADQYREIASTMPSEDERIACYNEFLLDRLENIMPGLLAEE
                     EFDAWMVIGRENNEDPIIRTLLPGSFYGASRITAFLFTKNNRFVLSPHGSQLSGYYES
                     AWKVEDGDIFDSIGKLLSDLKVRTLGVDYSDNFALADGITVSLFQSLKRKLSDEVTLV
                     SAENIAVNWLQTRSEKEIEVYRNLDRIAHVIIRNAFSRDNITPGVTKTRDIELLLKKL
                     AYDIGLKTWFGPDVDFQRKGVEDARCTGLIEEGDLLHCDFGFMYNGLATDTQQVFYLK
                     KEGYDDLKLGLAEVIRRTNEVQNILARNFREGITGNELLRVSLEDAKVKGLEAAIYSH
                     PVGYHGHGAGPVIGLWNNQDRISGAGDLKIRNMTCFAMELNCRTRLSLWDEQNVYGFL
                     EETVAFTDNEIDYLDGRQKELFLI"
     gene            48736..49410
                     /locus_tag="PKL93_05165"
     CDS             48736..49410
                     /locus_tag="PKL93_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNQ70685.1"
                     /translation="MKKTLTAFFYIALIITLGLAIELKESANSMSVNDLAILEIQENP
                     STGYLWHIFAEPTGVLRNFLEEHNAPSMMPGAPSVKGWIMTAAKEGKALITLKLFRQW
                     EGEKHSVDFRALTVDVAGQGKGQVDLKILMNEVTLGTTFTVTLEENASTGYTWQYVVL
                     GDGITEKSKEISVDKSDKVGAPSKVTWTFQAVDEGYSTIIFRYFRSWEGKESAVDYKA
                     YNISVE"
     gene            49459..51231
                     /locus_tag="PKL93_05170"
     CDS             49459..51231
                     /locus_tag="PKL93_05170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ70686.1"
                     /translation="MCIPGSRSKLQVYLTSILIVVVSAVVFSLHSIQPVKFFAILSEM
                     VVIGVSLVAAYLLGNRELHKGIPLGLIFLSISTYFDLLEALTETRVYEFVSVVLLVIG
                     LPMLLISAFYHIKERKKRISEFRAVVNNSLDGILLLGLSGEILHPNRAACSLLGYSSD
                     ELMKKQVSDLLSEDSEKKFEKAKSSLFAGKNAFQIELELITARQFKLVAEVNLVIGRD
                     TKEDPDSFLMTFKDVTHLRKIESTLIEQNKFQRLLNELITEVLESDFDEKTYRYFLMR
                     CVEVFPKADAAAFLLKKEDNRYHFVATHNYDFEKLKTVSFSSDELIQKGSGDVVIIKD
                     YSVDYQMDGERLEKIIKGGRLEEIKSTLSIPIKIDDDIRMYFNLDSFTSSTAFDDKEV
                     VNTAIGFGKAISVLLWRIQTVNELRRQRKLMEKLSLEDHLTGLPNRRAFFDCAERQVE
                     FSKRKSEAMALLYLDLNDFKGVNDTLGHDVGDELLKSVGKRLSTVCRRSDLIARMGGD
                     EFVYVLPSTGKKGSREAETRIHQAFEDPFSVKGRSLKLTASVGIAVFPDDGKTIRELL
                     IVADEKMYENKEKLSRESKRVTIT"
BASE COUNT        13936 a        10913 c        11253 g        15130 t
ORIGIN      
        1 caaaagtcaa gaggtaaaat aaaaaaatct tagataaata taattattat tggaaaatag
       61 ataagtagtt cttccaaaaa agaaaaaagt ttaatgtagt agatcatata gaattttgga
      121 tgggcatttg tcaactgatt tgatgattaa gaatccaaag attcgaggat tgttggaata
      181 gttatgatcc agactttata atttctaatt ggaacatgga gaattgagat tgatcaaggg
      241 cagtaggtag agtctgcaaa aacatggaca tcaaatgtat aatcaaggaa agacaggggg
      301 cacttgcgga atggttaaac gtgctaaatg aatgcataat ttgtggttct gaaacgcgag
      361 agatctataa tcgaacattt aaggtgaaat atcgccactg cggaaactgt gagttcatct
      421 cgaaggatcc ggccgattgg atttcagaag aagaagagct gaagatctat aataggcaca
      481 ataatagcat cgaggacccg agatacgttg cgtttttcaa agatttcatc gattctgcag
      541 ttgtgccttt cgtctcaaat ggaagaagag gatttgattt tggaagtggt ccatcgcctg
      601 tgcttgcaac tatacttgaa agggattacg gcttcaaaat ggacatctat gatctttact
      661 tctcgccaaa tgaggtcttt aaggggaaaa aatacaatct aattacctca actgaggttt
      721 tcgaacacct tgcggaaccc gtaaagtatt ttaaaatgct ttcggcctat cttgaggatg
      781 atggattgct ttcagtcatg actctttttc atccacaaga tcatcagcag tttcttgatt
      841 ggtactacat gagagatatg agtcatatat ccttttatac tgtcagaaca atgcaagtaa
      901 tttccgatct tgtcggactt gagttgttat tcactgataa aaagagatac acttctttca
      961 aactgagaaa gaagtgagga agacacctta atggtcagaa acaaaacagg ccctctcgtg
     1021 aagagcctgt tttctatttg ggaatttccg aacaggggtg gggtctagca tgtctctatt
     1081 tgatcgagtc gagaaggagt tgattcttcg atttatttta gtcaaaaatg tagaattgtc
     1141 aattaaccca gcgtgactat tgtttaagtc tatatgtact ttttttcttc cgactcagag
     1201 taagttacaa cattcttagc aaacttgaaa atcaatcgga tttcctggca gtaattaatc
     1261 tcttcattgc ctcttcaact atggcccttg ggcaggcaag attcaccctc atgaaccctt
     1321 cgccttcttc accgaatatc gagccgtctt caaagccaac ctttgccttt tcgtacagaa
     1381 agctcctgag ctcttcaaca ctcatacctg tatttctaaa atcgatccat gcaagatagg
     1441 ttccttcgag ttcagcaact cttagctccg ggaaattggt tttcaagaaa tctctcatat
     1501 acacgaagtt cccattaatg tattcaagta gttgctcgag ccaacgttcc ccattgttat
     1561 aagccgtttc aagagcgact gtgccaaaaa cgttagacat accggcccca actgtgctca
     1621 tcatattcga gtattctcct agaagttcac tggatgacga gattaccgct gctgaagaga
     1681 ggcccgcgag attgaaagtc ttacttggcg caacgcatgt cagtgttatg gaagaaacct
     1741 cgtcagaaat cattgctgta ggtatatgct tgttattgcc aaaaaccaga tctgaatgga
     1801 tctcatcaga gattatcttg atgtttctgt tcaaacagat ctctcccagc cttgagagtt
     1861 catctcttct ccaaaccctt cctactggat tgtggggact gcagagaatg ataatcttcg
     1921 ttcttgaatc aacttttctt tcaaggtctt cgaaatccat tgagtacctt ccgtctgatt
     1981 ccttcaaagg gttcagaatc aattgtcgac ccgtttcttt gattgctttg aagaaaggat
     2041 aatacaccgg tgtctgcaca accaccctgt ctccaggatg agaaagcgcc ataatggcaa
     2101 tgtgaactgc cgcgacaact ccaggagcgt gtgcaatcca ctcttccttt atcttccagc
     2161 catgtcttct ataaagccaa ttcacgatag actcgtagta agcttttgat cttgcggtat
     2221 atccgtagat tccatgcttt gctcttgaaa ctattgcctc gacaacttca gggggcgact
     2281 caaaatccat atcggctacc cacatcggaa gcagatcatt ccttccaaaa aagagatctg
     2341 cctgatccca cttaatgcaa tttgttcctc ttctctcgat aactctgtca aagtcgtatt
     2401 tcacagcttt cctccatctc ttagaaatgt catccatgtt cacattcaag attatctctg
     2461 gttgagctga aagaatttct ggactactca ataaacggaa gaccatcacc cgagtaaaga
     2521 tattctggaa tctccggctc gggatattcc cccttgattt tctctttgat cacgttgaga
     2581 tagttttcag cacttgatcg ccatgtatat ttttcggtta cccgatcaac tcctcttgaa
     2641 cttaattctt cccaaaaaga gggactgctg aagagagcct ttttcaatcc gcgaacaata
     2701 tcatttgttt caagaggatc tactagcact ccatattcaa tgttgtcttc tcttaaactc
     2761 tctgccggtc caccatttga ggttgctact actggcagcc cgcaggccat agcttcaatc
     2821 ggtgcaaggc caaacggttc atacagagat gtcaaagcga aaatcgattt cctcttcgta
     2881 gagattctat atagggcgga tagttcgtgc tgattctcaa tgttaaggaa aatcactccg
     2941 tattcaattc catttctgcg gatatggtcg atgatttcct tcagaactga acgatcaggt
     3001 tcctcaaggg agtcgaattc ttcgtatgga tttttaatcc ctctcgtgac gatcacaaga
     3061 ttgcttgact catggagttc acgatcattt gcaaaggctc tgactattcc aatgtggttc
     3121 ttctttcctt cgaatctgct tgagacaacg atcatcggga gtcgaaacct cgatgcattt
     3181 gaaaatcttt caacagcctc tttgtatcta tcttctattg cttcgtccaa ttccgatgga
     3241 tttgcagtga atatatccgt attgacaccg ggaggtacga ttgagtattt tgaatcgttc
     3301 ccgacgtcag aaaactcacg gtaaagggga tggctatact gctgaaatct ttcgatcgaa
     3361 gttgaaacaa aattcactgc agagtacttc atggagatac gctctgccgc aattcgatag
     3421 gagaagttga actcctcttc gattttcatg cggttttctc ccgtctgaag tagcttatct
     3481 agtttctgag cgcctaagga gtgagcggtg aacgagtaag gaatccccgt ttctttgaat
     3541 aacatagcgc ctgtaagacc tccatctccg taatgtgttg taagaaaatt cggcatcgtg
     3601 cgctctgctc gatagaactc tattattctt cttgagaact cacctaggta gggccataag
     3661 ccctcttttc ttagaaattc gtcggggccg aagggaatcc gaacgattct gagattctct
     3721 tttcctggat agtagtcgaa ttcatcggcg aattcgggcc atctctcatc gattattctt
     3781 ctggtgatta tgtcacacct gattcccatt tccgacatag ccgaagcgac ctctttgaca
     3841 tagaccaact ggcccccaaa atccggatga gtcgtccagt aactgtcttc tctatcgaag
     3901 tttccctgcg ggtttataaa cgcaactctc acaaattatc acctgcctcg aaaatcgcct
     3961 tcagagcatc tatagaaggt aagatcactt cctttccttc gtgttcaatg cggaagcccg
     4021 cgcagtagtt accgtacacc gccgattcaa atatgttttt tccaccgagc aatccgaata
     4081 aaactcctga ccaaaatgca tctccagctc ctgttgtatc tactactctt ctcgcacaag
     4141 atggcaaggt cactaccctg tttccgtcgg atatcattga tcctttttgc cccagagtta
     4201 gaatgacgtt tttcgctcca gattcatgga atttgcgaat gtaatcaaga ggttttccag
     4261 ggccgaaaat ctcgttagca tcatcttcag aaggtttgac aagaaagaca ttctcgagga
     4321 acccttttat gaattccgaa ccatcctgat tgtgagtcca cagaatttgt ctgtagttgg
     4381 gatcgaatga gatccttact cccgttcgaa gtgcataatc tacaagtttg cagcaaacct
     4441 ctcgagacct ctcgcacgat agcggccatg cagtgaagtg aataaagtct gtgtccttca
     4501 tgaagctatc aatatcatac ggttcctcaa gtaagcaatc tgcacctctg aaggccgaga
     4561 actgtggtga tgactgagat ttcgaaacga agaccacaga ggtatgggct tcctggtctt
     4621 gctggacaaa ggaagtatct atccctttta aacgaagatg ctcaaggtaa gcccttccga
     4681 aaggatcatt tccaactctt gatagaagaa tactttcgat acccaggtcg ttaagattcg
     4741 atgcaaggtt tcccggcgaa cccgcaaaga accttcgaaa agaagtcgct ttgcctagat
     4801 ctttcacagt attggtagat atcagatcaa taaggagctc accaacgatg gcaattcgag
     4861 acaattcaac gcctccttct cttattagtc tatcatttga gagtctattt ccgttcacgg
     4921 atccgggatc tgctcaaaca tagctcttca gaaaccagat tctcaaagtg ggaggcgtgt
     4981 ataatcaagt taacaatggc tacacatatt tgagacagga ggtttagtaa atggaggaaa
     5041 tatctttaaa aaggctccaa agaagatatg ttggaagtga tattgattta tcgctctggg
     5101 agaatctgga aagcgagctc aagaggcttg aggaatacga aattgttgat agagattcgc
     5161 tagagaactt cctgcttcac tggagtgagc tattcatgat tcttcaagag aagcttgcgt
     5221 ggaagtacat cgacatgaca agatttgctg ataacgagaa gaagagactt gattattcca
     5281 aattcttcga agatgtttat tcgaaggcag agccttatag aataaggctt atgaaaaaat
     5341 actacgatag cattcacaga agggagttag atcctttccg atatgagaac ttcgatgcga
     5401 tagtttctaa cgcggttgag cttttcaatg aggataatct ctctctggag gttcaggaga
     5461 agaaaatggc gtctgaatat gcggcgacca tcggttcaat aactgcagag tttcgaggaa
     5521 aagaatacac tctttcgcag ctatcgaggt tccagctgga accggaccgc ttaactcgcg
     5581 aagaagcctg gaggctttca atgagcaaac ttgatgaagt acatgaaagc cttgagaacc
     5641 tatttgatag acttctttct gtaagggttc ctcaggcggc aaattctgga ttcactagtt
     5701 atagagacta tatgcatcag aagaagaaca gatttgtcta ttcagtcgag gatatacaca
     5761 gatttcatga atctgtagaa aaagttgtag ttccttttgt ttcggagctt aatgagaaga
     5821 gacggaaaga actcgggttg gatagactaa gaccctggga tattagtgtt gagccttcgg
     5881 gaagagttct aaagccattt gaaacaatgg aggaattcat acagaaggct atcagagtac
     5941 ttcagaaggt tgacccggag ttcggcgcta atctcttaaa gatgaagaaa aacggtctgc
     6001 ttgatctcga aaacagaaag ggaaaggctc caggtggcta caactacccc ttagatgaaa
     6061 ccggcgctcc tttcatcttt atgaacgcca cgggaactcc cgcaaatgtc aaaaccatac
     6121 ttcatgaatc aggacatgcg atgcactctt ttgcaacgac agatgagaaa ctgatagctt
     6181 atagacacgc aacccacgaa gctgccgaac tggcttccat gtctatggaa cttttgacga
     6241 tggattactg ggatgaatac tactcagaag aggaggtaag gattgcaaga agagaggaac
     6301 tgaccggaac tttgagtttt ctgccatggt gtatgattgt ggattctttc cagcaatgga
     6361 tctatacaaa ccccgatcat tcttcctcgg aaagagatga gaaattctct ttggttttcg
     6421 atcgatttaa cactggagtc gactggacag gactcgaaga agagaagaag attcgctggc
     6481 ttcttcagcc acatattttt acgaatccct tctactatat agaatacgga attgctcaac
     6541 ttggggctct cgctgtttac agaaactata ggaaaaatgg ctctaaggcc attgaggatt
     6601 acaagaagtt cctttcaatg ggttattcca ggccgctaga cgaattgttt gaaagcgcag
     6661 gtataaaatt cgatttttcg gagcattacc tgcttgaact cgtccgattt gttaaaagcg
     6721 agctagcttt gctttgaaga gagtgcttct ttgaaagaag ggacagttcg caagggtagc
     6781 ttcggcgaag ctcacagagt tagaattggt gaggaggtgt ttggatgaga aaaatcgtct
     6841 ttgttatagt tctgttgatg ataccgttcg ttttgctggg tcaggtagaa gaactcaagg
     6901 ccaagatagc cgaaaatcct ctggatgttg aaagtctcaa agcattgctg gaaatctact
     6961 atgagaatta cgaacttgag agttatggaa caacactgaa agaagtaata atgaatgttg
     7021 atgagattcc cgaagcaatg tatccgttga ttgaagaagg aattgagaag ttgcttgaaa
     7081 atgcttattc agattacgcc gaagaaatat ccgagatcct ttttgatctt cggccagaca
     7141 agatctctct gctactctat cttaagtccc tgaatagcgg ctgggccatg tcggaggagg
     7201 ctattcgaca gtctgtaaac agactcagcg aggatcagat cgagatttac gagtttctcc
     7261 atgaggagtc tgttgacctt tatccagttg atcttcatct gcttgtatcg agaatccttg
     7321 tagaggattt tggagagact gactatttga ttccttatat agtggggttg atagacgatt
     7381 acgacttttc aaccgctaga agtctgctaa gtgagaattc agaatttctt agctcttcac
     7441 cgatgtacta ctatctgaat ggaattcttg atctggagac aggctactat gacgaagctg
     7501 ttgaatggtt cctgcagggc aagtccagag acgatggtac tgagatttac gaagcgcttt
     7561 ctttgatcga gtatatatat atacctgaat atgtaaccga cttgttcact gcgatcgtac
     7621 tggcaaacga cgaggcatcg ctaacttcga atattcagga gtatgttctc aactacgagg
     7681 agttgtcctg gatcttttca tggatttcaa gagttccaga acctaagaaa ctggcagaga
     7741 ttctctcaga tgaggagact gccgtatcgt acaacacggt gaacttcgat ttcgagaaga
     7801 tcaacattgg attctacgat gcctacgcat cacttctggg aacagtttca agtgctattt
     7861 acggacagtt tctagatgcg agaacttatg tctacatatc tgaaaagatg gatggagtaa
     7921 tagttgaggg agagagagaa gccagatttg acggatatga tgtttattct ctttccccca
     7981 agagagaccg attattgatc atggatactt ggggagagtc aatctcaatg gtagatagca
     8041 gcatggatat tctatggaca ttgtctgaag agtacagcat ggcgcctgtc gcgtggtcgc
     8101 cagatggatc taaagttgtt gtgtcaacgg gcggagacta cttctcagta atcgatgtcg
     8161 aatcggggca agtcatcgag tcaatcgaga gtttctatga tctggtcttc ttgtctttgg
     8221 atgaaactta cgcggtagac tacgatggga aaattgtcaa tcttacaacg gaatccgttc
     8281 tcgatacaga tgctccagtc tactgggctg catacggcaa taatgacaag ataatctatc
     8341 tcgaataccc cgacgaatat tcggatttct cttacgattc tcttcatgta tatgacttga
     8401 gcaccgggga tgacagcgtg atagcttata acgctatgtt tatggatgcg ccggtgcctt
     8461 ctgtgaagat agagggagat ttcgtttatt tcacagagaa ggatgttcag ggaatgcaca
     8521 gggtaattgt tatggattac acgacaaacg aggtgctgtt tgagagtctg cccagtgaag
     8581 atataataat cttccccgat gttagacctg aatgacgaaa cgaaggccgg cttagccggc
     8641 cttccttttt ttgtagttca gagtaaatga tgaatttttg atagagatct cttagagttc
     8701 tacttctttc ctgcaaaagt tttatccagc tgttccttgg ttattagatc ggaagagacc
     8761 cctttctttg aacaacagta cctggcagct atctggcctc tcagtattga ttcctctaaa
     8821 gtatatcctt caaggaagta actcgaaaga aaccccgcag caagactatc acctgctccg
     8881 tttgtgtcga ctacaggttc atcaagctca accggaccga agaagttgat tccgtgctcc
     8941 gtgcctaatg cacatcctct ctttcccatt ccacagatca caattctatc gtttcgaacc
     9001 ctaaggtatt ttgatattat gggggtggga tctcgaaagt ttgttgatga gaagaagaga
     9061 acgtcagcct ctctaacata gtcttctctg tacaggtcat cgggatccgt tacatcctgg
     9121 atatctacag aaattacaag ccctgcttct cttaaaggtt ttagaagaaa tctggaccag
     9181 ttcacaatat tgacgtgggc aagtttggtt ccaaataggt atttctttat ggcttcaaca
     9241 tccggcttca aggccattgc tcctctaccg tcatagaagt tctttcgctc accgttcttg
     9301 tacataatgt ttacgcttct cttagttccc aatgggtcgg tcttaaggaa agcgatatcg
     9361 ataccctgtg cagaaagagt ttcccggatg tggtctccaa aatagtctga tccaacgaat
     9421 ccaacaaagc aaacctcgtg gccaagcctt ttcagcgact ggcttacata tcctcccgcc
     9481 tggccgatgc aatcgactac ttcagtaaat gttgcctcta ccgaaaagtc tatctcgcta
     9541 cccgataaat agacgtttgt atcaatcccg atggcgccaa ttacagctac ttcgaagccc
     9601 ataaaggatc actcctgttt aaggacgctt ctgaaaagct caagtttctc cttgagcaat
     9661 tctcctccca gaagaccgga gagagccttt tcgtaatcct caactcaaat tcctggaatc
     9721 acatttttgt gagcaatagt tccacaagaa ctacattttg catccgggga tcctcaaaac
     9781 ttctctcaat actgaaatca cctttgaatt gtgattcctt gtctcccgtt cgatgtcatc
     9841 cttatttact tcttttatcc ttctcaaaag gacatccggt ggttgccaaa ccgtgtcata
     9901 gtataaccag tattcccccg tctctaagtt aattcaccat tcctcaatgc ttcatgacgc
     9961 tcaaaggtta tcaccatttg agcagggtgc ttcttgatca ataccgaatg aacattattg
    10021 ccaattctta gctgagataa aaccataccc ctcctctctg agccagtcca taggtatcgg
    10081 ttccgatagc ccactgaccg gagtggtcga cggtcctaag aagaatttcg ctgagcagtc
    10141 ctatgcactg tccaccaact ttaattatac aaacgtcaaa cctcctcaag ctttcacccc
    10201 ttcactgatc atcggattca tttgactctg agggacagtt tgaactcagg atctatcctc
    10261 gatacagaga tcgtgttgaa accaaattga accgtcttag gcagcttgat aactcggaca
    10321 ggcaaagtca gcgatacata gtttctgtta catttcattc cagttttaac aggatttggc
    10381 gatggtttat ggctcctttt cagttctcta gtgaactcca gcatacatga atgcttcacg
    10441 ataatcaccg tgaagccttc ttcttcgaca gcccgttcaa caagaagcta gttccccatc
    10501 ataatctccg aacttctttc ttgggagagg agtatttcgc caatcctctt cataggatcc
    10561 cacctttcaa aagatattct ttcatgtcac gcgaaatcaa tttcccagaa aatgtcagct
    10621 acgcgggttt ttgttttgta tactgaacat tactcatttt ttgttcgaaa gacttctcca
    10681 acgaacttct gtaagatgta tccacttcac agactctgac ctttatttga caaagcttca
    10741 aagtagctgg attctcgtcc ctcttttctc cggataattc gatttcagtc gagacaatct
    10801 caattctctt ctttttcaga atatcgagaa atgtggcgta aaggcattat gccagactca
    10861 ttaggagtag atcataggga gcagatcctt cttttccaat tcttaccttg taaccggaat
    10921 cggtaattcc agaaccggag aagccatctt ttaaatctac ctttgcgtgt actgtaattc
    10981 ccatttcttg cctcctctgc attttgattg gaaaggagtt attagaacta gatgtcacga
    11041 agatcaaacg ggcagcgttg ctagaaagga acgaaactca tggttcgcga caactacagg
    11101 ttgcacagtt tagatcataa caataatcaa tattaacatt caaatgtcaa gaaaaaccag
    11161 aatacgagat tgcctcgaag tctctcgtga gtgaaaagta gaatatatta ggaggtgcga
    11221 tgtgaatcta tacgcgttgt tcaattcatc tctttatcat ggaacttctt cgagaaaagg
    11281 tttttttgaa ggctggtact tcaagtttgt tgacaggaag aaggaaaagt catttgcagt
    11341 cataccggga gtgtccttga attcgtcgag agaagatgct cacgcttttg ttcaattcct
    11401 ctcgggtggg aagaatgaat ccggcaactt caactatccc ttgaaagagt tccattctga
    11461 taagaaacac ttctctgttg agatctcgaa aaattatttt tcggccggag cggtgattct
    11521 ggaaatgtgc aatgagaatc acagaattgc tgggaacata aggatcttgg agccgtacct
    11581 ttggcctaga aagttctatt cccctgggat catggggcct ttttcctttg taccattcat
    11641 ggaatgttac cacggggtcg tcagcatgga ccacaaactt gagggagaag taagaatcga
    11701 tgatgaactg atagatcttt ccggaggcag aggatatata gaaaaggatt ggggaagatc
    11761 atttccagag gcgtgggtct ggatgcagag caacagcttt gatctcgaga gaacctcatt
    11821 tatgctttcg attgcaacga ttccatggtt gggaaggagt ttcaccggtt gcctctgcgc
    11881 atttctgttt aaagaaaagc tctataaatt cactacctac aggggagcaa agatcgatag
    11941 agtagaggtt ttggacaacg aagtattcct ccaactcaga caggcagggt tttccttgaa
    12001 tgtcagtgca aaaaagactt ctggagcgcg tttaatctcg cctgtagaag gtagtatgag
    12061 tgggaaaatt gatgaatcgc ttacgtctga aataacggtg agactcctcc gaaacgatgc
    12121 ggtcttgttt gaaggtaccg ggacaaattc cggcctagaa gttgttgggg agtttgagct
    12181 gaaggaaaaa agtaaatgag aacctgtgct gttcaatcat tagaggactg gatattgttg
    12241 ctaaggggat tcttccgttc ctctttagca gtttatctct tacatgtctg tgaaaaaagg
    12301 gcatccctag tttcctggat gccctctaac ataagattcc gaaaaaattg tttccaaatc
    12361 ctctagccac ttcttttcag cacccctctt tttttcgccc aaacctccat tctcttgaaa
    12421 aacacgaggc cgaatggaag taggagtgcc ccaagaagaa tcagaggcca aacatcattc
    12481 cacatctccg gcatggttct gccttcaagt atagatgctc gcatacctct taaagcataa
    12541 gtagcgggag atagcttgcc tacttgttgc atccacatcg gaagcacatc gatttcgtag
    12601 tacacacctg agaacatcag aagcaaggcc tgaaagatat gaactacttg aacgcctttt
    12661 tcaggtgaga tcaggggcaa cacggcagcg acaattccca gtccaacgaa tgaaaaactt
    12721 gaaaccgcca gtattccggc cgccgacagg aaattggctc ttccaagatc tagatcgaag
    12781 aagaccgaga cgactactag aataagacca cttctaagta ttccatatag aatggcatat
    12841 ccacacgtac tcaatagatg agtagctctt ctaacaggcg ccatgaaggt gtattcgata
    12901 gtctcttccc atctctccca ggcaacggtc tcggctacta tctcgaagag aattgaaagg
    12961 tagccccaca gtatcgatcc aagcaacatg taaaggatca ggtagcttgt atcaagcttc
    13021 gtccctgaaa aggcttcaat tccctttccg atgaaaccca ttgtcacaga gtttgccatg
    13081 gtgtaaacaa agaagaccag ttcccacttc cagtatctct tcgtgaaatt gaaattccgt
    13141 tcaataaagg cccaggaggc tcgaagttcc cgggctaacg cctgcgaact catgctgact
    13201 caacctcctg atattcggcc tcttcgatac tcattccggt aaactcgaga aagacatctt
    13261 cgaagcttgg attgtcaact ttaccgagca actgcttctt cagttctgag ggtcgtccag
    13321 aagcaacgat tcttcctaaa tgaattatcg caacgtaatc acagagtttc tccgcttcat
    13381 ccatgtcatg agttgtcaag agcatggttg tattcatggt gttcttcaca tcgctaatta
    13441 gactctgaac atccctcttt gcccttggat caagtcccgt ggtcggctcg tccaaaagaa
    13501 gcattttggg ttcagtcata aaggccctaa caatggctac cttctgttgc atacctctgg
    13561 aaaagtcttc tagcggatct ttcaatctct tgagatccag tccgaatctc tcggcgagac
    13621 cgtttatctt tttgagagcc tctcgccgtg gaattccata tagtcctgct gaaaaacaga
    13681 gattctccac tgcggaaagc ttcctgaaga aactcgcctc gaccgaaact ctgtttatca
    13741 ttctttgaac cgatgcagaa ttactaacgg aatcgtaccc gaaaatggat atgcttcctc
    13801 tatctggaat caagagtgtt gaggcaattc tgataaaggt tgattttccc gaaccgtttg
    13861 gacctagaag accgaaaatc tctccttcct ttattctcaa acttatccct tttagtgctt
    13921 tgatttcctt tctactcttc ttaaatgctt tcactatatt gttggcaagc aaagcgtctg
    13981 ccatgttctc acctccctaa aggtaatgag gtctgctgaa cctgctctcg atttctatct
    14041 tctgcttatc aaccaagcca tctataaaaa cgctctttga gcctctgatc ttatttttca
    14101 ttgatatctc ctcctgtttt ctaccttgtc ttggccagtg ttactaagct tttcggtttt
    14161 tcgataaaag gcgggagggc cgtcgtttcg atttccctct tctttcttgg cgatccaata
    14221 acgatgaaca tatttacccc ctcgatattc ggagagaatg gcccgtctaa gtatccaaag
    14281 aacactatcg atatttgccg gattctccgt tttggttctt tctgtttctg ctgctaatct
    14341 gctttcgttt ctcggtttcc ttcaaaaggc ttttctcaaa catttctgca cctccttagt
    14401 gtcaaaaaaa aggaccgcgg ctgttgcaag ccacggtcct tcataatcca attgtcgtta
    14461 tacgatctta tacctcacac gacggaagaa ggcgtggcac agtacggcat cgccggatac
    14521 ccgattaagg gaaacggttg ccggaacgta agctctcatg gtcatcaaac tgtgcctcct
    14581 tcgtagtaaa tgtacctgtt gctaaggaat tatagcataa tgggggagaa attcaaatat
    14641 caaactctaa gatttcctaa agtggttctt aaatttgata accttattca cacgatgact
    14701 aaataattct tctcttgtcg atgaatttat gtctaaagta atccaaagat caaggcagtt
    14761 atcaaacgtg tccgattgac tgtatagaca attctgttca ggctggtcta atcaaaaagg
    14821 tctcgatcat ctaaatgact ggtcaagaag ctctttgttg ttttcgatca catcgtctgt
    14881 gaatggatca ccgggaaaga gaagaatctc gcccttgccg taaacatgcc tacctgactc
    14941 tccccgaaga acggaaagtc catcgaacac gattccgtcg agttcaactg catcactagt
    15001 aatggctttt tcataggttg cataaattcc catctcttcc aagagcctat tgtaggaaga
    15061 gataatttca ttgctgattt catctcctgt gcctgttata atcagtctcc caccgttcat
    15121 aacaaattcc tttatgatat tcgattgctg aggcattagt attttcagac gatcaaaact
    15181 cccacctgct ccaggaagtg gaagtagaag gacatctata gtatcgagtt caccaacttt
    15241 gaagatccta tctgcggtgg aaaccctgtt gcccatttcc tcgagtagca tcttcagttt
    15301 ttctcgctcg tcacttgcgt aattgatgtg actcctatcc accagaacat tgagagaggc
    15361 agaacttctt atctctaacc aggtgctcga atacggaatt gaatcaagaa ggaaaagtat
    15421 ccttgcatca tgttcttctg cggttttttc gagatgaact atcttcgatt cgaatccctc
    15481 tagcgtcaca atctcttcgt ggataatccc tcccagattc tctatctgaa gcaaagaaga
    15541 agcttcttca gaggaaaggt ttgagatctg gaatgaaaga gtcactcttt ctccgggctt
    15601 gacactttcc ggaaatgcgg ctgaattata gaggtgaatc gggcaagatc tttctaccca
    15661 aatcggagaa gaaactgctt cctcgccgtc atggcctctt gcgtatacga aaaagtactg
    15721 aaatccaact tcaatatttg ttgtcatctc aatatagaac tcgtgactgt ctacaagaaa
    15781 atttgcaatg accccgtcat ttgagtagat ttcgacaatt tccaaaggat catccgcggt
    15841 atcacgtata agtattctaa gttcaactct atcggcatca cgtactatgt ctcccatgaa
    15901 tgcttcctcg gttgaaaaat gaatctcaat gttcctgtct tccgtcgcgt atgttcttct
    15961 cttcttaaat gcttccagta tcgatttttc gctcagatcg gttgcgaaga cggctgttct
    16021 ggagtcattt gcacctcccc aattaggttt gtggttatcc tgaccaaggg tcgtaccaag
    16081 atgccatcct ttacgaagtg cttcccgata tgcactaaac atctcttcga caatcgtatc
    16141 gttttcccac cagcttccat tgcctacttc tataagattc atatagagat cggcttccgg
    16201 ggaatactga aattccttga agttgtcacc gaaatctctc actggatgat tgaattgacc
    16261 gacggcatca tgctcgatta tccagtcata gagctgccag agatcggatt tgactctatc
    16321 ggtccaatca cttgtgccgt aaacgttgat gtgtcctgtt cctgttgccg tccattcgaa
    16381 ccccacaaat gctaggaagt cttcagatgt tgcttcaatg gccgcctcta ttgttgcagc
    16441 tagtttgttc ctgccatcag gaagatcttg agcgaaataa taggcatgat cggttattgc
    16501 tagaaagtcg aggtccggga cttccttggc gtaggaataa gcctcttccg gaacacctga
    16561 gccatccgag taagaagtat gggagtgggg gttcccgaag taaaggttga gattgccgcc
    16621 aactgaggcg acagttgata aaagaagaag cgccaccaat aaaaaccttt tcatcaaatc
    16681 accctttcac aatcaacgct tttttatgta agatcactaa agctcaagag aatcaacaca
    16741 tcttgctttt tttatctgga taggatatca gtctccaatc tcttcttcgt tttcgatttt
    16801 acctaagtat aatctgcttt gaagcatctt tgagaagtct gtccatctgc tttcttttga
    16861 agcagaaaaa gcatggcttt caaaattgtc aagccttgaa tcaatattgt cacaaagcca
    16921 tatcagtatt gcttctcggg ttttcggtgt taccggtgaa ccgaactgca gttccccgtg
    16981 atggctcaac actatgtgca ttagctttct tctggtttcc tccggaaagc cgctaatttt
    17041 ggagacttct aattcgatca acctggcgcc aatagcttat gttcttctag ataaccatca
    17101 tcggagtaat caaacttgaa agattccgtt atgtaggtcc ttgtctttcc aatgtcgtgc
    17161 agaaaacccc ctgcaatgat tagatctctg ttaatgtttg gtctggagtt cgacgcttct
    17221 cttaaagcta gtgcatatct aacaacagag agactgtgtt cagccaaacc accgattctg
    17281 gcatgatgat agacttttgc tgcaggcgct tcaaagaagt cagggcaagc cccatcatca
    17341 gaaaccatcc gctttacaag agtgttcaga tatttatctt tgatcgactc tacaagttcc
    17401 agaacttcag attttatctt ttttaagtct tcaacaattg gaagcagctg ggttatcgag
    17461 cctgagctgt agctttccat ttcacttatt gagaagagct tcctgatttt tatgctcaat
    17521 ccaaatctgt catctataag gtagtctgca agacctatcc agccctcaac cggttccaaa
    17581 cagccctgtt cactgatcca tgcctttgct tcagttctac acttagaatc ccctacgaga
    17641 cagtcatagt atgggcctct gcttccctct atcctctttg cactgaatat gaagaagaaa
    17701 ccttgctttg aattaacata gttttcaacc ctgtcgagcg cagagttgta ttctcttagg
    17761 atttcggaaa gcacgggata acttccactt tttccatcaa tcatcgtgtc ttgctccttt
    17821 tgcaacattt aaaactctct gaaacgagtt tcatagtacc ggtagtcaga aacagactag
    17881 cctgaatctt ttcttgatgc agacttcatc agcagtttta tcattatgaa gaaggcgaca
    17941 gtagcaagaa acattgtcgg atagccctca acaaagatca ttatgctttt ggaaacggct
    18001 tcattcatca cttaactcct ttcatccaag aaagagtgcc agaacaatgc taccggccag
    18061 tacagaacct atctgacctg caacgtttgc tccggttgca tacattatga gaaagttgtt
    18121 cggatcttct tccagtccca tacggtgaac aactctggca gacatcggga aagcagaaat
    18181 tcctgcagca cctatcatgg ggtttatctt ctgctttctg aaaacgttca agatcttggc
    18241 gaaaacaatt ccacccatcg tatccagtac aaaagcgata agacccatca agagaatcaa
    18301 caaggttctt gaattaagaa atgcttctgc agtcatagtt gagccaatgg caatcccaag
    18361 aaagagagtg ataatgtttg acagttcgtt ttgagcggca ttactcaact tgtttagtac
    18421 gccggactct cttataagat taccaaacat cagcatccct attagcgcta cacttacagg
    18481 tgcaataatt cccgctaaca gagtcactag gatcgggaaa agtatcctca cgtatttggg
    18541 aaccttctcg gtcctgttgt ccattcggat ccttctctct tccttagtgg tcagcagttt
    18601 tattattggt ggttgcacaa ctggaacgag agccatatat gaataggcgg cgactgaaat
    18661 cggtcccaac atgtggattg ccagtctact tgcgacataa atagacgttg gaccatcggc
    18721 tgtacctatt atccctatag aaatcgcttc ccttatgtca aatcccagta acgctgccac
    18781 aataatggtc aagaagatcc caagctgagc agccgctccg taaaagaaca tgacgggttt
    18841 ctgtaataga ggtccaaagt ctatcatggc tccgattccg atgaatatta gggctgggaa
    18901 aagttcattc gcaatcccgg cctcgtaaag aatagtgagc acaccatgct ctccaatagc
    18961 cgatgaccca ggaatatttg taagaatagc tccaaacccg ataggaagaa gcaacattgg
    19021 ctcatattcc ttctttattg ctaagtagat caggattgct cctattgcaa acattactag
    19081 attccctaca ggaatagaaa agttcgatag tagattcact ggtgatgcct cccgaattgt
    19141 atttctaagt ttcacattcg acttttgtgt gaaaggaagc ggattgcccc tttttcaaat
    19201 acaatattaa tacatacgga aggcttttga tcgggtcgaa aagtctttgt tactaatttt
    19261 ctgaaaagca aacaaccact gctcaattct ttgagttgtg gtaggatttg tcagttgagg
    19321 ttgatctcaa ggaggcggtc gctttgaggg cagtatttcc tgagggtttt ctctggggtg
    19381 tagcaacggc ttcctatcaa attgaaggag ctgatttcga ggatggaaag gggccatcga
    19441 tttggactga tttctctcac agacctggca gagtagattc cggagacaat ggtgacgttg
    19501 cttgcgatca ttatcaccgg ttctccgaag atattgagct aatgaagcaa cttggtatta
    19561 attcatatcg attctcaata tcctggtcga gggtgctccc cgaaggtcga ggaaagataa
    19621 accgcaaggg ttcggatttt tacaacaaac ttatcgatag acttctggaa gttgggatac
    19681 aacccatggt cacactgtat cactgggatc ttcctcttga gttgcataag aaaataggtg
    19741 gctgggagag ccgagatatg agacattatt tcggcgatta ctccagcttg gtgttcagcg
    19801 aatttgggga cagggtaaaa cactggatca cgctgaacga accttattgt tcctctcatg
    19861 taagctatct ctggggtgaa catgccccgg gcaagagaga tctgaaaact tcacttatcg
    19921 tggctcacaa cctgttactc tctcatggag aggctgtgag acgttttagg gaagtcgtca
    19981 aagatgggac tattggtctt gctaatgtct ccactttcgt cgaaccagcc accgatagca
    20041 aagaagacag atgggctgca agaattcgag atcagttcat taacggatgg ttctttgaga
    20101 cacccataac aggtgagtat ccttctgaac tcttcaagag attcaacgat gcaggagtac
    20161 aaccgctcat cgaagacggt gacatggatt taatatcgac gccatttgat ttttggggtg
    20221 taaactacta cacaagaaac gttgtaagaa agaaagaatc aagtattcta ggtagtgagg
    20281 ttattcaagg tgaacttgct aagacagaaa tgggatggga agtctacccc gaggggcttg
    20341 aagcttttct ttacaagaca ttcaaggaat acgggaaaaa accaatatac attaccgaga
    20401 atggcatggc gtgcaaggac aagttgactg atggctttgt tgaagatttt gaaagggttg
    20461 actatatgaa gaggcatttc tcttccgctc tcagtgcttt gaaggcagga gtagatctga
    20521 ggggttttta tgtctggtcg ctgctggata atttcgaatg gtcttatggc ttttcgaaaa
    20581 ggttcggatt agtgtatgtt gactacgaaa aaggtcttaa acgtatcccc aagcgcagct
    20641 attactatta cagagacttc ctcgaacggg attgagaaag ctctcttcta agaaaaatac
    20701 tgccgggttc catttgtggt tttcatagtg agccctgctg aacggagaaa agataccaaa
    20761 ggtcttataa agagctgtga aagagtttgt ttgataggag gttttgaaat gatcggagtt
    20821 gtcgtggatt caggatgtga tcttcctgat gaggttaaag caaatgacaa tgtaagagaa
    20881 gtgccactga agatagtcct ggatggtaag gaatatcgag acaatattga tatttccact
    20941 tctgatcttc ttgagtacat ggaaaacaag tttccgaaga catcacttcc aagaccgatt
    21001 gacatcgggg aagctttcga aagcctttat gaaaagggat acaacgagat cctcttcata
    21061 gggatttcaa gtggcctgag tggcacactt gatcaggtta agtcttatgt tcgcgatttt
    21121 tcagaaaaac atggcgattc gaaaattgaa gtagtggatt caaagaatat ttcaattggg
    21181 tctggtcttc tggcaatgaa agcaattgag ctgatcaaaa gtggggagtc ttttgaagga
    21241 gtcgtcaaag acattctggg ttctgtcagg aaagcgaagg ttttcttctg catacccgta
    21301 ctgaagtatc taagagcagg agggcgaata ggaaaggtta ccgccactat cggtgatatt
    21361 cttgatctga agcccgtaat tactgtcgga gaagacggtg tttatcacag cgtttctaaa
    21421 gaaagaggta tgaagagagc tgtgaaaagc tccgtcgaga aactgattca tttcatcgac
    21481 ggcaataggg tgctgaacat tgcggcttat acttctgaca agagtgagaa aaccatggag
    21541 tttttcagga tggcggtttc agagattaaa gccgctggat ttagccatat aataaccggc
    21601 cagatatctc aatctcttat ttgtcataca ggtcccggtc tgatcggagt tgctgcgatc
    21661 ttggagtaaa aaaaagccgc cgaagtggcg gcttaaaaat cgggatattc cagggggttt
    21721 ctcatgcctt gtccgtcatc cataacatgg gggtaatggt gaacaagcat attgttgctt
    21781 aggtgttttg tcttttttaa tgaccgattg agctttgggg tgcaagggtt tcttatctat
    21841 ctgatataat tctacccaaa aatatcgggt ttgtcaatat actggataat aattatcggt
    21901 ttttctttgt aaacattcgg taaagtcaaa atctatattg tgtaggacga actgtgatcc
    21961 gatgatttag ttatatattt tgcttagctt tccttgaaga ttgcaattat tggtggtaga
    22021 gacatttagt gctctactca gccggagatg tatctattgg acaggaacag tcaaactaga
    22081 attctatcgg tttttgaggg tgccatttac aatgggttct tcttaattac tcagggattc
    22141 cttgggacgg ggcttgctct tgaatttggg gcgtccgaac cagttattgc tttgattggc
    22201 gttctccctt cagtttcaca attaattcag ctcattgctc cttttatatt aagactggtt
    22261 ggaaacagaa agaaagtaat gatgatatgt gcgtctgcgg ggagactatc gacggcgttt
    22321 attccagtaa cacttgcatt aggaataaac agacagtcac ttctactgat aattcttgca
    22381 ttcttttcat ttgcagcaag tttggcaggg aatttctggg tatcgattat cagggatgtt
    22441 gttccgccag ataaatcggc aaggttcttc agtctgagaa acgttatatt cacaataaca
    22501 aatatgttga taacgcttct ttattctttc attcttgacc attcatctgg aaaaacaggt
    22561 tttgtgataa taaccactat gggggcagtc agtgcgttgg tcagccttgt tctactgggt
    22621 cttcacaacg atcctccaca cgagataagc tatgggagtg gcctttttaa ggctgttaca
    22681 agagataaga aattcatgac ctatctccgt ttctattcct tctggagctt ttcaattgcg
    22741 attgcttcac ccttctttgc ctatcatgag ctagtgaata tggaactgga ttattcgttt
    22801 ctcagtttac ttaacatctt tagctcaact cttacgatga tgttttatct attgtggggg
    22861 aggatagctg atagaattgg aggacaggcg gtattggaat tcggcatctt cggtgcattt
    22921 ttaaagacat ttctctggtt gtacatggac cggggaacgg tctatttgct ttacatagat
    22981 acttttattg gtaccgcttc gtggagcgca ataaatcttt gtcttttcac tacaatgctc
    23041 gaattactaa atggggtaga tgcggaaact tactatgcgt tgctttcttt ttcgaatggg
    23101 gcttttgctc ttgctggttc actagttgga ggggttcttg cttcttatct taacaagttt
    23161 acctggacac tcggtggaca tacgtttttc ggcattcaga ttttgttcct agttactctg
    23221 gtcttgagag gtgtttcttt tctgctattg aagagagtaa agacgaagaa agtcgtatca
    23281 gtacctggaa tggttttcaa ttcggcagtt gtcatagcta atcgacttgc agcgaggccg
    23341 agagagttta ttgaagtgat gatagagaag aacaaatcaa agaagaagga gtgataatag
    23401 tgattgagaa attgattgag cgatttctga gatatatatc gattgaaacc acttcaagct
    23461 ttgaatctaa gactttcccg tctacaactg gtcagctagt tcttgccaga actctcaagg
    23521 aggacatgga atctatggga ttgtctgaag ttactattga cgattttggt tatgtgatgg
    23581 ggaccctgcc ctcaaacctc gaaaggaaac taccagtgat tggatttatc tctcatatgg
    23641 atacgagccc ggaaatgagc ggaaaagatg taagaccttc aattgttaaa tatcagggag
    23701 ggaagttgct ccttaacaag aaagataacc tctatatcga tcctcaactg tatccagaaa
    23761 ttgaaagata tgttggagaa gatctgattg ttactgatgg aactactttg cttggagcgg
    23821 atgataaggc agggattgca gagattttaa ccgcgatgga atttataatt tcgaatcccc
    23881 ggattaaaca cggcaaaata agagtagcct ttacaccgga cgaggagatc gggagaggaa
    23941 ctaagcattt cagcatagaa gaattcggag ccgattacgc gtacacagta gatgggggag
    24001 gcattggaga actgcagtac gagactttca acgctgccaa cactgaggtt gtgattaagg
    24061 gactgaatat ccatcctgga agcgcgaaag ataggatgaa aaactcaatg ctcattgcat
    24121 gtgaattcaa cgcaatgctg ccctcaaatg aaataccggc caccacttgc gggagagagg
    24181 ggttcttcca cttaatcagt gttcaaggtt ctgtagaaga aacaatacta aaatacatca
    24241 ttagagatca ttccagggag aagttttcga gaaaaaagat gatgatttcc gaagtagcgg
    24301 ctttattgaa cttcaaatat ggagaagata cggtcacgtt ttgcactgaa gactcttatt
    24361 acaatatgaa agagaagata gaggaagtgt attttgttgt tgagaccgcg aaaagagcaa
    24421 tggagagtct ttcaattgaa ccgaaaatag aacctgtgcg agggggaact gatggtgcgc
    24481 gtctttcttt tatgggactt cctacaccca atctcttcac aggaggacac aattttcacg
    24541 gaagattcga gtacatacca ataagatcta tggaaaagtc ggttcaggta attacgaaga
    24601 tagtagagtt gttctctgag gaggcgacct ttgaaagtta ggactctttt atggaagttc
    24661 aaagtctgga gacgaagatt tgaagttaaa gaacgatcac cttaacccag tataactcta
    24721 cagctttttt cggtaagagt cacaccccaa tatagagatt tatgttcaaa gacgttagat
    24781 ttgctttgat tcgcacgatc tttgtagatt atgcgaacat tcgtggttct tagctcaagg
    24841 agtcttgtaa gtaagactct acagattttt tgggatctct tggaggtgaa agatgtctac
    24901 tagttccaga tttgtgagag aaagaattgg aagagataac cctctctttt caatgagccg
    24961 tgtaacaata gaaacagctt tctacggaaa caacgtagta ccggttgttt cgccgagtga
    25021 ggcctacaag ctcgcgaaga actcggcagg tacgatagtc actgaccttc cagtttataa
    25081 acccgaaaaa gccggtcttg agtcagattc aaaagttctt ctcttcaatg acggtgccgt
    25141 gaccggaaga tgtgcctcgg cgaggcgaat agtgggagag ccaggagtaa acgaaacaga
    25201 atacggagcg aagttgcgag aagcagtcta caacacaaga aataggaaga tgtatcatgc
    25261 ccaggcatat atagggctcg atagagattt catggtaaaa gcacatcttc tggttcctga
    25321 aacccacgag aacctgattt ataattggtt gttaaatttc cagccgataa acgaattcta
    25381 caacgaaatg tatgagtcct cagaaaggtg tgaaaacgag ggcgatattt ttgtgttttc
    25441 cgacccggaa tggcatcatc cagatcatcc attggggctt tcattcttcg atccggaaca
    25501 caattgcgcc gcaattctcg gaatgagata cttcggtgaa ttcaaaaaag gaacgcttac
    25561 ccttgcttgg ggatgtgcca acaggcatgg ttttgctgct tgtcatggtg gtcagaaaag
    25621 gtacaatctc caggacagaa aatttgttgt gggtttcttt ggattgtccg gttccggaaa
    25681 gtcaacgctt actcatgcta agcacgacgg aaagtatgat gttacagtgt tgcacgatga
    25741 tgccttcgtg atttcatctt ccgatggatc ttccgtagca ctggaaccct cgtatttcga
    25801 caagacgtcc gactatcccc taagtagtga agacaacaag tttcttcttt ctgttcagaa
    25861 cgttggtgcg acccaagacg atgaaggacg aatcgttatt gtgacggaag acattagaaa
    25921 tggaaatgga agggcaatca agtcaaaaat ctggtctcca aatagagtgg acaaattcga
    25981 tgaaccggta gacgctatca tctggttgat gaaagaccca tcaattcctc cggtagtgaa
    26041 agtcaacgat cccgttctcg catctgttat gggcgcgact cttgccacaa aaaggacctc
    26101 tgcagaacgt ctggcaccag gagttgatcc caatgcgctg gtaatagagc cctacgcgaa
    26161 tccattcaga acctatccgc tttctgacga ttttaatcgt tttattgaat tgtttactca
    26221 cagagggatt aaatgttata ttttcaatac cggccatttt ggcgagaaga aggttccgaa
    26281 ggaactcacc cttcaaatcc tcgagtctat agtagagaac cgagcagagt tcttgccgtg
    26341 ggacccattc aaggaactcg agataatgtt tattgaaggt tttttgcctg atctttctga
    26401 tgagagctac aagaagcttc tgaaggagag actcgttgac aggtacaact tccttcggtc
    26461 tagagaagtt gaaagaggtg gcttcgacag acttccccag gaggcagtcg aatcgatttc
    26521 gtcgttgatt aaaatggtag agtagttttg gttttatcat ggggccgcct ttggcggcct
    26581 tttcacttgt ttatttaatc ttagtttaca ataaaaaagg attcctgact ggcgtcagga
    26641 atcgaagggg gaattgctac gaaacgagga gttgtccgga ttgcggctaa ctttaccgtt
    26701 taaacgagtg cattgtgaat acactgtaac cgaagttaac cgttaacctt tcaaacttat
    26761 tatacaattt attctcaaag aaatcaaatc gtctctatta tgtaaatgga gacgacattt
    26821 gcatggttca tcagtcggtg cctcagttag ccttagttta aggtattgtg cagttgacaa
    26881 acaagcccgg aaaataccgg gctcgtagaa cggtagtaaa caatcaattt gttacttcga
    26941 tggtataaga ttttatttga taatcttctt tattcattct ctttcgggag gttttgagtt
    27001 tgagggtatt ggttacgggc gctagaatgt ggtatgcaat taatactata aggtttttgg
    27061 ctagaagcgg ccatgaggtt ttcgcagcgg acagcagtag acttagcggg ggtctttact
    27121 ccagatatct caaagggaaa ttcatttatc cttcagtcgg tgagaatagc agtgcatttg
    27181 tcgaaagcgt cattgaaaag atagaagaac tgaagatcga tgttctgtgt ccaacctttg
    27241 aagagggttt tgtgttcagc aaatatattg gtaggttgga aggtcgtgta aatgtactgg
    27301 tcccttctta cgatcatatt actctacttc acgacaagta ctcaatgacc acttacgcaa
    27361 gatcacttgg cataagtgtt ccaaagacag tactgttgaa ggatttcagc ttagaagggt
    27421 gtgaattccc ggtaataatt aaacccagaa atcagcgatc tgctgaaggt attttcaagg
    27481 ttaattccaa aagtgagttc gagaaaagat gcaggaatct tgaagaagac aaatatctgg
    27541 tccaagagtg gaaagagcct taccagattt gtacaacagg gttggctcat aatggtcgat
    27601 taattggaaa cgtcatttat cacaacataa gagaataccc tgaatctgga ggaattggta
    27661 cctgcagagt ctctatagat tgcgaaaaag cgcttggaga agttaagaaa atcgtcagcg
    27721 atctcaacta ctctggattc atttcgatgg atttcctaca tgacagaatc aatggtgaat
    27781 actatcttgt cgatgtaaat cccaggatga gccccgggct gctcattgct tataactctg
    27841 gtgttgacat ggtaaaggcg tatcttgaat tagcgaatgg aagcgaagtc agcgtggcta
    27901 gatcgccgaa aaccggttat ggtaccttta caacggctat ggagatcggt tggttcttca
    27961 gtacgttgtt taaaggtaag tttaagaacc tgaagggctt tttcaagagc aggaagaatc
    28021 taaaggatga ttcatgggat gttcgcgatc cgattccttt ttttgtgatg cttctgtcca
    28081 tgctgaacac tgctgtcttt ggacctctca gaggtggtca gacgaagagt ttttctctcg
    28141 gagcaacatg tgatacagaa agtctaggtt ccgaggagga gttgattaag aagaaagctg
    28201 tgtagaaatc aagccatggt cgtaaaaaac actgctatgg atagtaaacc ggtcttagag
    28261 ttggtaatag aagttctacc tcacaatgga aattttcaaa tggtccaaga caatgttaga
    28321 cagtcgattc atttgacgca cgaaaagtct attgaaacct gattcaagag actggaatca
    28381 atagtaccga acaattgttt cgaaagctag aagtagatca tttgagtatt aagattgggc
    28441 tcgccgaagg gtcttccaaa ttaaagcaaa tccagattca gcagctttga ctcatgacaa
    28501 atgtggtttg ttatatattg tcgtttgtag agctacagaa gtacgaattt ccgtaggttc
    28561 ttttgtattg attactcatt cccggaaaga actcgtctag ttttagatag taatattctc
    28621 tctgcctgtc cctcaaagtc attctcattg aaggatagat aaacttggtt ccagcttctt
    28681 ttcctctggt aagtattggc aagatgtctt ccgtactatc ttctgtgaat ggcaggatcg
    28741 gcatcaacgc gatttcgaca tcaatgcctg ctctagataa agccttgaat gcttcgattc
    28801 ctctcgaatg ccagggtact tctggttcga gtttgtttgc caacttatcg tctggagttg
    28861 ttatcgtgaa caccaccgag caacgcttcg agacattaag aatatcagcg tctttaattg
    28921 aaggtcgccc tttgtggtta tctggactcc atacgcgaga tctcgactaa ccaaaagagg
    28981 tctcttcggt attttcacct ttttggcgga gtacatgaat cgctctttta tccggtcgat
    29041 tctatgcgct tatctctttt acgtttgagc tctaatttcg gaaggtccac tgcatttgac
    29101 ctgtaacaga cattttcgaa accgtctact tagcattgac tcccagagtc acagtgtatg
    29161 cagcagtgct ggctccaacg atgccgatag cttgtagatc ttatacacct cgaaaactgc
    29221 ccgcctagag tggaaacaac ggtcttcgcg attatttctg tagtggaaca atgatactgc
    29281 tcaaggaact acgtaaatgc aagatcttcg aatgacaaga agcgtgactt tgcgagaaat
    29341 agaggcaatt tctccggttt atctagtttt cttcagaagt gaggtcaaag ggtacgaaga
    29401 caattatctg atataattta ttctgaagga atcgttgaaa gagagatttg cccagacgaa
    29461 gtgctcttct ggcgcttagc aggacagtga cgaagagtta ttttggagtg atgttatggg
    29521 ttttttcgaa gggtttagca agatagtatt tgtgatctcc tacgttttat gggctggagg
    29581 ggggctcacg gtttcgctct tgatgatacc tatgaaggat cccagaactg aacctcaggt
    29641 tcagggttac cttcacgctt tctggcaaac cacaagaata ccggcagcgg ctctttcttt
    29701 cgttgttttg ggagcagcaa ttacaatggc aataatcgtt tcagcttcct tgctttggag
    29761 ctcaattttc tctttgtcat ggtttctcac tttcttgttc tggcttatgg cggacaggct
    29821 tatgaaatca ggaggaagcg atccaagatc aatggtcgag gtccataaga gaagcttcca
    29881 cgcttatctg gttatggcag atatttcaat ctttctttca atgctttcag ttgcgtttat
    29941 gctgttatga ttgatctccc atcattgtag aaagttcctc taggtcgtgt tccaggatct
    30001 ctctttcttc aagaatctca agaatcctct cttcggcctg attcaaagtg ttctgaaatt
    30061 cttgaagcct ctcgtaatca ctagcgtaaa gccccattct tgcatgaatc tcttccagac
    30121 tattggacag agcttcttct tcagcatcca attgttgcaa cttttcaatg atcgacttct
    30181 tcttgttgga taatctcctt ctttcttgaa agctgactcg cgcgtcatca ttacccaccg
    30241 aaccggtata gctttgattt ctactcaaat actcttcaac agtggataca ctcttcaact
    30301 tatgcctatc gatcatcaag aaacgatcac aaatattctg tacgaattct ctgtcgtgag
    30361 aaacaagaat tatgcagcct tcgtattctt tcagcacctg ttcaaggctt tcaatggacc
    30421 agatgtcgag attgttcgtc ggttcatcca tgaccaaaac gtttggacgc gataatatca
    30481 gtttcgcaag cgcaagtttc gttttttctc caccactcaa ggtgtgtact ggtttaaaaa
    30541 catcgtcgcc gggaaatcca aaccttccca tgtactttct aacttcgtaa tctggttgcc
    30601 ccctcatcaa ttgccagatt tccgtgagca catcgtttga cggatttaga tcctcggtca
    30661 gctgagaaag atagccccaa cggatgttgt ggccccaggt aacttctccc gagtacccct
    30721 ttaaattccc agtaattatc ttcaagagtg ttgtcttccc gctgccattt gggccgagaa
    30781 ggccgatctt atctctcctg tgtatttctg tggtggcgct gctgaaaaga ttcttgctgc
    30841 caaagccgaa tgacaatttt tcgatcttca aaactgagta accggttcta tctggttgtg
    30901 gtagccggaa ttttgttcct ctgatttctt catctgggag atctatcgat tcgagctgtt
    30961 cgattagtct gtccctttgc ttttctttgt tgatcgcctg cttgatgaat ttttcctgcc
    31021 cccacaatct gtagcgtttt gcgacagagt caagtctttc gatttctttt gaaagattat
    31081 cccgtgttct caggccgctc tttaacataa tctctctatc tgcctgatat ctgtcataag
    31141 gtccggagaa atcccaaagc gagccgccgt tcagctccca gaagcgatta ccaacgtttc
    31201 taatcaagta tctgtcgtga gtaacgacaa gtagagctcc tttgtaattc tttaagaagc
    31261 ttactaacca ttcagtcgat tcgagatcga gatgatttgt aggttcatcc aagagcagta
    31321 gatcgtaatc aaccaggaaa agcttcccaa gagagatcct ggttagttcg ccaccactga
    31381 aggtttcgag acgtctttcc caatcttcct cgccgaattc aagccctacg aggatactcc
    31441 tcactcttct gtcgaaggaa tagtattctt cggtgtcggg aaggacacta gatttgtcct
    31501 gcatgtagta ctccataaga gtcaactcag agttgggtat tctattctgt atttgataac
    31561 caattctcac gtttgtggag aggaaaacct ctccctcaga cggcaaaaga tcaccggaaa
    31621 ttattctcat gagggttgat tttccgcttc cgttttttcc aagaagtatt gtcttatctt
    31681 tcctatctat cgaggttgag acttcgtcaa agaggaagtc ttgaccataa tcgtgtccaa
    31741 ctttgctcag agtaatcagc atacatttcc cttccaatct gccggtcagt gataataata
    31801 caacataaca gtttcttttg tgggaagggg tcttcatgca ttcatggttc gatgacatgt
    31861 gatatctttg attatgtggt gaaaccgggt ggtgaatttc tttgagactc aggctgcctt
    31921 ttcttatagt tcttacagga tttctagcgg tctcatacat ctggggattc aacttcttca
    31981 ggagtccata tcaagtcttc ctgcaatatg agagggcaat gatcgactat gcaaatcaaa
    32041 ttcgtcttca gcaagattcc ggccttgaag ccggattaag tgaagatgaa attgtgtcgc
    32101 tgagaaaggt agccattgtc gagcttgaag gagacatgag gaatctgata tttgacttcc
    32161 ataagtattg gtcaattatt gagaagtatt atctacagta tgcccaaagt tcaggcatct
    32221 ggttcacaga tggattatcg ggatcgatat atactcaaga cgatccagag tatgatactt
    32281 ttctcgctct ctacggactg aaggatgatc ctgaaagtta ctgcctgata tttgtccctg
    32341 aagtttccga agagagtgcc ttcagagagt tcagaggagc gcggatttac ttccttcaga
    32401 agacaatttg gggttacaag attgaatgga gcagatccct gatcgatttg attctggatg
    32461 tagattcctc gtctgtctcg gtgtaatctt ttgttctttc acagaaggcc gactcgcttt
    32521 tgagtaaccg ctccttagga taagccaacg ttctcctgac gaggttttct ggtatccttt
    32581 tataggagct attcattccc ttgttgctgg aggatatgat gttctttaag aagattaatg
    32641 atagaagaag agagaatagg attactaaga tagaaagaag gattccgaaa tctcgagaag
    32701 aggaaaaagg taggttcctg tatgaactct ggaatttgaa gagagaaaaa ggagataatg
    32761 aaggcgctat gaaggctgca ctagaaaggc ttgcatttct caataatcct gagtcatttg
    32821 aagaccttat ctcaaccttt gagaagattg atgggtcctc gaaaaactct tttgcccgag
    32881 aaatcgagaa gtttgcctgc gaatttgata gaggtctctg ggaagaattt atatcggtct
    32941 tctttgagga ccaacctgag gccgccatta agttcgcctt aagctgttac aggatctctt
    33001 cgaaagcatt atttgtcgaa attgctcttg ggaggcttga gaaagctttt tataacgaag
    33061 agcccggtag gcttgtgcgg ctacgagaga tgtacatttc aacaatttcg gaagtcggag
    33121 agatctctag agtcgatcag cttcgccttc tcgagagagc cttagagtct tcaaaatcgc
    33181 tgattcaaga cgaacaatta ctttccaacc ttgcggtgaa gtatattgat cttatgcttt
    33241 ccggcgaaga tttagatgga atcgcaaaga aattatcggg ttgttttgac cgctttgtag
    33301 tttatttcaa aggaaatgaa gtgactcttc ttgacaaact agatctcatt cttccgaaga
    33361 tacaagaaga agaaatcaaa agagctttga tagaaaagct tcgaatattg ggacagtcga
    33421 tatcggataa aagatacgac tccgattttt ctggtgaatc agattacctt gaatttctta
    33481 agaaaagcat tgctaactac gagtttaaag cgaggaaaag agattcgggc gaggagcttc
    33541 taagaaaact cgcctgtgat tacgaaagaa ttattgacat tcttgagtct cctgaggaga
    33601 agctgttata cattaggaag ctgggagcct tgcttcggga tcgccttgaa gaaccctaca
    33661 aagcacttga agtcttcgaa gaggggctga gattgaaggc tgatgacata tctctgtggc
    33721 ttgtcaaagg aatgaccctg gagaaaatag cgcgtaatga aagtgatcca gttaaggctt
    33781 cgatatgttg gaaaaggact tacgatcatt atcatgcgat tgcggagact tttgaggagc
    33841 aggaagtaaa gcttacggcg attgagaagt gcgcaacgat tcaggcagag agattgaccg
    33901 gagtttagct ttgttgagat ggttctttag ggatcctttg ggggtgcgtg ctttgagatt
    33961 cttgaacaaa ccgtgcttgt tactggtgaa tccaaaggaa tcgggaaaac aatatccgtt
    34021 ggtttcgcga gatgtggtgc atttgtgtcg attatggata tcgatgtgga agctgggaat
    34081 cgcttggtaa gccagatgat ttccgacgaa ggccgagcga cgtttattta tggagatgtt
    34141 tcttctgaag atgatgtaat gagagttgtt actggtacgg tgggactatc tggatcgata
    34201 ggcgttctca taaacaatgc ggggatagga tggaccggga cacttctaga gaggtcagca
    34261 gatgattgga ggaaagtaat cgaggtcaat ctcaccggcc cctatttgat ggcgaaatac
    34321 tgctctactt atttagccac ttcgaaaggc tcaagcataa taaacatagt ttcgactctg
    34381 gctttgatgt tggtaagcag ttcggaacca tactctactt cgaaaggtag tcagctggca
    34441 cacacacatt tacctgccat tattcttggg ccaaaagtca gagtcagtgc gacatgctcg
    34501 ggatagatcg acataactga aagccagccc agtaagccat ccgaacgaag cgatgttgca
    34561 cataatcaac acccggccgg gcgagttggt agaccggaag acgttgcaga tgcctgtttt
    34621 tttcttagcg gtgatgaagc ctcttttatc acaggtgcaa atctagttgt tgacggtgaa
    34681 atgacacgca agatgattta tctagaatag gagtgatcag agttgagtca aaggctggat
    34741 ggaaaaaagg ttaaatctct tatagaaatg ctttcgggat atcacagaat gcggggaagt
    34801 cgtgactata aggaatctat gaactcgata acggactatc ttatccgttc cggtcttccg
    34861 gaaaatagat tccgggtatt gaagtatcca gccgatgggg tcacaaagac gggaaacatc
    34921 gtctctacac ttgcatggga accagtgtat ggggagctgt ggcttgagaa tccagagagg
    34981 gttttcgtca cttcaacaaa agtcacaaaa gtctccctgg tttccggtag tggaagcagc
    35041 aaaggctggg aatcgttacc tctagttgta tatgacggta aaggagatta ctccggaaaa
    35101 gcagttcttg ctagtgaaga tcctgtcaag gtttttcagc atgcggtagt tgagggcggg
    35161 gcgaggtgtc taattctgtg ccatatgaga aaggtctttg aggaaattgg tcgcagtctc
    35221 aatgatttac ctcaaatgac aaacttcttg acgatccccc acgacagaga ttcggccgat
    35281 agaaacgctg ttgctttttc ggttacaaaa gagaagtacg atcttctctt caattactcg
    35341 aaaaaaggaa gtgcgatcgt tgggttcagg gctgaaacaa atatgtcgaa aggtagtttt
    35401 gatgttctgc aaatagacgc gactggtttc aatgatggcc ctcaggttct cataactgcg
    35461 catttgtgtc atccttcgcc tggggccgat gataacggga gtggagccgc tctcgccgtt
    35521 gagatagcaa gggtgttgaa tgatgagaac ttcccatatt ccgtgaaaat tgcacttgtt
    35581 cccgaatatt tgggaagcgt accttatgct ctccaactca agagcgagaa taaccttccg
    35641 atatatacta taaaccttga tatggttggt gcagatcaga acaaaacggg ttctacattt
    35701 attctttcaa aggtcccacc ttatttgcca cagaggtggg gaaatatcct ggaattctat
    35761 atcaagactc ttatgccttc aaatgccggt tatcctctga agagatttgg agagattccc
    35821 ttcatggccg gttcagatca ctgtgtcttc acaactcttg gaattccctc tccgttcatg
    35881 ggccatcttc cagatagata ctaccactcg gatttagaca caccccagat gatggatgaa
    35941 aaagaactgg agtgggtagg tctgtcggct ctcaatactt tggatcagct agtcaagccc
    36001 gatcaggact tgctcataag tgtcagaagc aagatgatag gagagctatt cggtatacta
    36061 aacagaattt cagggagaga aggaagcgat gatgttttcg atcttctgat ttctaattac
    36121 gagggagaag tactcagaaa ggttttcaat aattctggga atcttccttc actaagccca
    36181 ctggagccaa ccttcgatag ctctttaggt ctggaatgga tcaggacatt ccctcaggaa
    36241 cttaaagacg agctcgaaat tgatttcgca agtatcgccg atttcgtggt gggaggtgcg
    36301 gctgttgttg gaggtaggga agccgtagaa ctattggcaa gtattcacta cggagtcgag
    36361 atcgggaagg ttagggtact taccggctgg atgatcgaga aaggtttgct gagatcatga
    36421 acatcagggg attttgttta attatctttc ttatcttaac agcgtcctgt tttgcaaatt
    36481 tggagatgaa ggtagatagt ggtgtatcaa tgccgtggag tgggttcctt gaagtcagtg
    36541 attctgagag aacccagctc ctgatgaaaa acgacttccg cgcttatttc ttaccaactg
    36601 tggggtatga gatcagcggt gaacatctga tagagttgag tttttctcac ttctccgtta
    36661 aaagtacatt cgaggcgacg gacactgacc ctttgtcttc atacgaatca atgtttaagc
    36721 aggatgtact ttcgctcact tacatgtata tacttcctga gcttgatcgc ttctctcttc
    36781 agctgggtgt tcaaggtagg ttgtggttta aacaactgac gctcactggc tcgaaaacac
    36841 ttgaccgcaa ggtttctgat ttgtcctttg gccctgctgc ggatttgagg ctgcagatga
    36901 acgataaagt cttcattgga ttagaggtct ctactggact gggaaaaggg aacggattca
    36961 ttgacatgtt cgctggactt cactttgacg ggccattcta ctatgttaga gcgggcacaa
    37021 gatattggtc tgtaacgaac tcctttgaaa acgatcgctt cttcctgtct tttgtgaatc
    37081 tgggggtgca gttctgcgca gttttctaga ctccattttt cacaaacgaa agcgggtcga
    37141 gtgaagagat tcttacaatt cccaggacag gaacgccaaa cttcgccagc aaagttcgac
    37201 cctcttcgaa ggtcttttcg atgattgccg caaagccgtg aagcgatgct cctgttttca
    37261 atacaagccg ggccagggcc tctatggtct taccgcttgc cagaaagtca tccacgataa
    37321 tgactctgtc tttctcatca atatagtcaa gggaaacgtt gagttccact acccctcctt
    37381 ttgtgtgcga aggagcggat tcactcagat acctttgcat tgttattggt ctactcttac
    37441 gagcaaaaat gagctgtgca ccaaggctgc tggcggccat caatgcggga gcaatcccag
    37501 aactctctgc agtcaagact tttgtcactc cataagttct gaagtagtca gcgattagtt
    37561 caccagcatt tttcattagt tgtggatcaa tttgatggtt aagaaaggaa tctactcgaa
    37621 gaatgccatt gcccagaacg gagcccttgt ttcttacagc ttcttcaaag gaagaaaata
    37681 tctcttgtct agaaagaaca acttcgtcca tcgattaacc tccaaaaggt atatggtaca
    37741 atgatcaact atttctgtat ccttttcttg aaggagaaat tccttttgtg taaggtatgt
    37801 tctccgaaca gatcggaact gcactgtaca ggaattaaaa atcatggtgc tcggaatcgc
    37861 atagtgtatt cttttcaaga ggtctggttt tctctccagt gataccaatc attcaaaagg
    37921 ccccgattca tcgtgattaa atctaccaaa aagtcttttt ccatttcttc ttgaaatatt
    37981 ctcagtgaat ggatactaca gactgggctt tgaagttttt ggcgcttcca aatccggtag
    38041 tttgagtttc actcctacca tgctttatgt tgggccttca gtatcatatt cgggtgcact
    38101 tggaaaagtt tcgggactag aagcagggat tgatgcacga ttgctgacgg gaatcgagaa
    38161 tctttctttt aacttgtttg gaagaagatt catgttcgcg ctcggactga gaggacaagc
    38221 tatgtactct ttcttcgaaa caaccgataa tttcaaaaca agactctccc ctgaaatcag
    38281 cttcgtagat gtgaccaaaa tgacggacag gttcaagttc tctctctttt tctggccgct
    38341 tcctgccctt ttgggtttcg acatgtattt ctaaaaaagg agccccaaaa ttggggcttc
    38401 ttttatttct gcacaagctt catcggctct ccacagcaga ctagttctcc tccaccagct
    38461 tctttcactt caactatgtt tccacatatc tcacatctgt agacttctcc aacgttcttt
    38521 accttcatga tctcactctc ctttttggta gtaaacttag ttactgtttc cacagtcaaa
    38581 tgatttggag gtgctgacaa tggaaagagt ctttttcgcc gttcttttca ctatattcat
    38641 tgttgccacc gcaggtaccc tctaccagga accacaggcg atcttcgcca gtcatcttca
    38701 agatggtacc cttgtttatg gcggtagtgt gatgcttagc tgggacgtcg ttgtcagaaa
    38761 caactctatc atattgagca acttggacat atgggtttca aacagagatt caggagctag
    38821 agttgtctta gaatcttctc ccttcatcga aattgccagc tacaatcttt atctgggaac
    38881 atctccgacg ccaaagcttt atgcatctac tggatcgaat agttacttcg tcgatagact
    38941 ttcaaatgat actgtttatt attggcaaat agttgccatt gacaaaaatg gaaatagata
    39001 ttatggaccg atatggtatt ttgaaacgag ataatcagac cacagcattg ggaggttcct
    39061 ttccttctag aactgcggcc acgttgcgcc ccaccatgat tgccatttct gtccttgctt
    39121 cttttgttgc agaccctacg tgtggaagaa ggaccacgtt atccatatct atcaaagact
    39181 gtggtacaat tggttcttct tcatagacat caagaccggc ggcagctaat ctaccattct
    39241 taagagcctt tatcagtgct gcttcatcga cgatggatcc cctggcagtg ttcaccagaa
    39301 tcgcaccggg tttcatcata gaaagtcgct tctcattaag caaatgatgt gtctctcttg
    39361 taagggggac gtgaatagac acaaaatcag attctttaag cagttcctcg aggctcctgt
    39421 gcttcacgcc aagtttctct tcttcttcag ttagaagagg tttcctgttg tgataaatta
    39481 ctttcatccc aaacgcttga gctctttttg caacggccct ccctattctt ccaaggccga
    39541 taatgcccaa attcttctct ttgagagagg gacctgttaa caggtctggc ttccatccgg
    39601 caaacctatg ctctctaact aatctgtcgc cctcaacgat tcttctgctt gtagcgagaa
    39661 tcaaagctaa cgttaagtcc gccgtggcgt ctgttagaac gtcgggagta ttggtaactc
    39721 ttacacctct ttttctggct tcttcaacgt cgatgttgtt atagccgacc gcgtaatttg
    39781 caatcacctt taatttcgga agtgatttga taagctctgc atcaatctta tccgataaca
    39841 gtgttaccag tgctaccgca tccttggctt tctgaataat ctcttctttt gagaggaacc
    39901 tatcctcagt gttgatcatg atgtcgtatc ctttaagaag aagcaaacct tcgtcgggaa
    39961 tcttataagt gaagaagatc ttgtccatta agcacctcct agaaaaatga tatcattact
    40021 tctgtaccat taggccaaga aaaggtactc tactaaccct cttgtgcctt caccaagatt
    40081 ccggacgcgt ctgagtcaat aataactttc attccttctc ccagggctcc atcgatgatc
    40141 aaatctgcta taggcccttc caccaacttc tcaattaatc tcctaaccgg tctagcacca
    40201 tattctctgt cgtaaccctc tttagcaaga tactcgaccg ctttctggct gatctcaacg
    40261 gaaatgtgct gatcagatag tcttttttcc atttccgcta ttctcatctt aacaatttcc
    40321 tccattgctt cgctatctaa aggtctgaaa agtacaatgg aatcaagcct gttaatgaac
    40381 tctggcctga aggctttccg caggctttct tcagcaagat ccattgctcg gctcagttcc
    40441 gaggtactcg ccagctcttc agatgcaata ttactggtca ttataattat tgtgtttgag
    40501 aaagaaactg tctttccttt tccatcagta agacgtccgt catccatgat ctgaagcaaa
    40561 acactgtgca cctctctgtg agccttctct atctcatcaa gaagaacaac tgaataaggt
    40621 cttctcctga ctgcttctgt taactgaccg ccttcttcgt aacccacata tccgggaggc
    40681 gctccaataa gtctggagac ggtgtgcttc tccatatatt cagacatatc aattcttacc
    40741 attgcctctt cactgccaaa gaggatgaat gcgagtctct tggcaagttc ggtcttaccc
    40801 acccctgtgg gtcccatgaa cagaaaagag ccccagggtt tatgagctgc atttaaaccg
    40861 gctcttgctc ttctgatcgt ctgcgaaacg attttgactg cctcttcctg atcgacaaac
    40921 ttttcgtgga taattgactc gagatcacgg agcttctcac gttcagactg aagcattttt
    40981 ccggcaggaa ttccagtcca ttgttcaaca attgaggcca caactgctga tgttacggtc
    41041 tttccaaaag actcttccga ttcccatttc tctttttcaa ggaggaattc ccttctcttt
    41101 ttctctgcca cggtctttaa ctcggcagcg gtccgatact cgcctttttc tgcatgagca
    41161 cttatctcgc catcaagctc cctgagctcg cgatcgagcg atctcagttt ttcgggcata
    41221 tagcctgttt gtagtctcac aaatgaagca gcctcgtcaa cgagatcgat cgctttgtca
    41281 ggaagatagc ggtctgttat gtatttgaca ctcagatcaa cggccgtctt cagtgcatcg
    41341 tcttcaattt ttatatcatg gtgtttctca taagtgggtt tcaacccttc gaggattctg
    41401 taggcctctt caactgtcgg ttgttctacc ataacgggtt gaaaccgtct ctctagcgcc
    41461 ctgtcctttt caatatactt tctgtattca tcgagagttg tagctccaat acactgcaac
    41521 tctccccttg caagggcagg tttcagcaga ttagcggcgt ccatggaacc ttctgccgaa
    41581 cccgctccaa cgacactatg aatttcatct atgaagagaa ttacttcgcc ggagagcttt
    41641 ctaacagagt ctattatacc tttcattctc tcttcaaatt cccctcggaa cttggtccca
    41701 gctacaagtc tacccatgtc aagggccaga acacttttgt tattcaaata ttccggcaca
    41761 tcaccgctca caattctttg cgcgagaccc tctacaattg cggttttgcc aacgcctggt
    41821 tctcccacca gggcagggtt attcttggtc tttcttccaa taatctgaat tactcttctt
    41881 acctcgtctt ctcttccaat aacgggcatt agcttgcctt ctcttgcgag ctgagtgaga
    41941 tctatcgtga atttcttcaa agaagagaga ttctcgtctt cctctgcatc gcctttcgta
    42001 cgacccttaa ggatcaaagt gtatatattg tcgagattga ctccgtactt cgagagcaat
    42061 ctgctggcca ctgcagaggt ttcccgtacc atggcaagaa gaatatgctc ggtccctgtc
    42121 tttgaatcgt gcattcttct agcctcgctt ctggcattct ccagaatgtg ccttgcatcc
    42181 ggagtgatgt atatctggtt accgatctga cttccgctcc cataacgacc gattacctct
    42241 tcaacatcct ttttgagaga accgatgctt accctctttt cattcaaaat gtcaattgca
    42301 atattgtcgc catcctccag catagagagg agaatatgct ctgaagcaag ctgattctgt
    42361 ttgtaccgca ttagaatatc ctgtacatcc ataaggatct tcttagcttt ctcggtgtat
    42421 tcttcatagt taatcatcat tacacctcca aaaaaagggc gcccgtaagc gccctctcag
    42481 tttaaacttt ttctcagtcg atcttgactt gaattgcttc ctttttgact tcttctttct
    42541 tggggagctc gagcttaagg acaccatctt cgtatttggc tttgacttca tcactcttta
    42601 cgtagtccgg aagtctgaag atcctctgga atgctccgct atatctctca tagcggtggt
    42661 aatctctttc cttctgctct ctctcaagtt tcttctctcc tttgatagag agaacatcat
    42721 cctccacctt gatttccagt tcatccttct tgattcccgg gatgtccatt tcaatgaaga
    42781 cagagttatc cttctcgtag atgtcaactt cggggctaaa catggttgtt tctcttgact
    42841 ggtcgctcag ccctttgaag gcgtcgttaa agagtctgtc catttctctc tgaatctctt
    42901 caaagggtct gaaaagatcg cttcctcttc tgattgccaa catattaaca cctccatttt
    42961 ctgggtttct atagattctg ttacttgctg tcttccggcg gtatgtattc ggtaccagtg
    43021 tcactgtcat gattttcacc ttcctgtggg ccaccatcag ctggagcctg tgcctgtgcc
    43081 ttctgataaa cgctttgtcc gactctcata ctctctttct ggagctgatc aaacaggagc
    43141 ttaattctct gaatattgtc ttcgcttaaa gcatccctca gatctttcac cagtccttca
    43201 agtttgcctc tgtcttcgga cgaaatctta tctccacttt cgttgagaag cttctccatg
    43261 ctgtatgcca actgatcggc ctggttcttt agctctactt cctgtttctt ctgcttatcc
    43321 tgctcttcgt atttctttgc ctcttctacg attctcttaa tatcctcttc agacattttc
    43381 tgtcttccac taacaaccat cgactgctct ctgttcgttc cgagatcttt tgcggagaca
    43441 tgtacaattc catcactatc gatgtcgaaa gtcacttcta tttgaggcac tcccctaggt
    43501 gccggtgcaa ttcctgtaag cttaaagctt ccaagagaca tgttgtctct ggccatggtc
    43561 ctttctccct ggaacactgc tacttccact tcggtctgac cgtctgccgc ggttgtgaaa
    43621 accttagact ttttcacggg aaccgtggag tttctttcga ttatcggctc cattaaaccg
    43681 cccatgacct cgactcctaa agtgagaggt gttacgtcta cgagcaccag atcgctttcc
    43741 acgctgccag agagaatacc gctctgaagc gctgcaccaa ttgctaccgc ttcgtctggg
    43801 ttgatgttct tgttaggatc tttgccgaag atcgacttga tgaagctttg aaccattgga
    43861 attctagtag aacctccgac aagtaacact tcatcaacct cttttggcga aagctttgca
    43921 tcgttcagtg ctctttctat ctgttccctg gtgccttcca caagatcttt tattagggat
    43981 tccaatgtgg accttgttat cttcatttcg aggtgcaatg ggccatcagc cgttgcagtt
    44041 atgaaaggca gggaaatctc agtttcatac tttgaagaaa gttcaatctt ggctctttcc
    44101 gcagcgtcct tcaatctctg aaaggcttgc ttgtcttttc tgaggtcgac gttgttcttc
    44161 ttcctgaagt cctcagctat gtagtctatt agcctctggt caaaatcatc tcctccgagg
    44221 tggttgttac ctgaagttga aagaacttca actactccat caccaatatc caggacggaa
    44281 acatcaaaag ttcctcctcc gagatcatat acgataattt ttttgtcacc ttttttcttg
    44341 tcgattccgt atgctacggc tgccgctgta ggctcgttaa ttattctctg gacctcaaga
    44401 ccagcaatta ttccggcttc tttagtggcc tgtctctgag catcattgaa atatgcagga
    44461 caagtaataa ctgccttagt aatttttcca ccaaggtaag cttctccatc tgccttcaac
    44521 ttcttcaaaa tataagcgct gatttcttga ggagtgtact ctttgtcatc tatctttact
    44581 ttgaaatcgg aacccatctt tcgctttatc gatcttacag tcctctccgc attcagaatc
    44641 gactgtctct tagcgggctc acccacaatg atttcaccgg tcttgctgaa cgatactata
    44701 gaaggcgttg tacgatttcc ttctgcatta ggaattacct cgacatttcc gtctggtttg
    44761 acccatgaaa taacggaatt agtcgttcca aggtcgatac ctactgtgta ttccttattt
    44821 gccatctgat ttccctcctt gcatgcaaac tcaacatcta caacactatt ataggcaaga
    44881 gaaatcaagc tgtcaacacc aaaatgtcat aatgtatatc tctgataaaa ctgattgaat
    44941 gagcactata aaagactaaa taataatgca atactattgt atagcgttcg tatcgtaaga
    45001 ctgataacaa gaattaggtt cttttagaat taccccatgc aagggttcga gaaatcggat
    45061 gaaatcagtt tatttggaca agaataatga agctgacatt caaggaaacc cgcatagaag
    45121 tattaccggt tactctaaag caagtcctga agagaatcga tatcggttac ttccgcaaca
    45181 gaaggttgct ctttaagaac tgcagggtat ttcaagccgg agcccgttag aactgcaaca
    45241 acaatagccc tcttgacatc aatcgctttt ctcagctttg aaatcgaagc aaatgctacc
    45301 gcgctggcag gttgtccaaa gagacctctc ttggctagtt gtctctgagc tgtgaggatc
    45361 tcgtcttcat taacggcaac agtgattccc tgattttctt ttagcattct cagaacagca
    45421 tttccgcttg gggggaaggg attttcgatt gcgtgtgcaa ttgtgtgtgc gtgggtaaac
    45481 ttttctatct gattgtcgtt tgaagaaaag gcttggtgta tagggcagca tcccaaagcc
    45541 tgtgccacga tcaatcgggg gatgctcctg gagagcccac atgccttgag ttcgcgaaat
    45601 cccttttcta ttcctctaat atttccacca gaacttgttg gaacgatcac aaaatcggga
    45661 actctacctt ccagctgaag gaatatctcg aatgcgatag ttttcgatcc ttctaccctc
    45721 attgggatgt cggaatttgc aaagtagatg ttcttctttc ttcctagctc cagtgatctg
    45781 aaataaagac ggtcataatc tcctttgacc ttcagtattc taggaccata gatggcgatt
    45841 ggcgagattt tctcctcagg gattgaatca gatactagaa ttgtggtctt cagtcccgcc
    45901 ctcataccgt aggctgcaac tgaggcggcc atgttacccg tggagactgt gccgatatgc
    45961 ttgtagccct tgttgatggc gtcgagaacg gccaaataac ttccgcggtc cttgaaagac
    46021 catgtcggat ttattgcctc gttctttatg aacagttcta aaccgtctgt catagcatgt
    46081 tctatcttcg tcaggggggt cagcccctca cccaatgaaa gagagttaaa atccttcagt
    46141 tcaggtaaga gatccgaaaa gctttcgaga attcctgatt ttttccagga gatgtttaca
    46201 gcctttttct tttgcatgat taactcaagt ggttccccac acgaacagcg gaaatgttct
    46261 cctaaatatt ctttgccgca catggtgcaa atcattttcg tttctctcat cttcatcctc
    46321 ttctaaatgc ttatcttaca cacttcataa tccatctttt ccttcaatcc aaggttggtg
    46381 gcaatcgaca aagaagggaa attctcagaa tcacagtgcc agtcaactac caacccgctt
    46441 gaaaggaatt catcgacgca tattttcgac acagccagac ccataccttg atttctaaac
    46501 tccttgatgg tttctaggcc cacttcgatt ctatctccat atctgtatac acccaagcat
    46561 cagctaatta ttatcttacc cttgattaat gcgtaacccc caccaagttt ctcgaatgaa
    46621 ttcagatctt tccaaaagga acatacccag cctctaagaa cgttactgtt atccaggtgg
    46681 cgattcagca acgcttcatt aattctgaat gcggtaaatt caattgagtt gtgtctttca
    46741 gtattactta acatggagtc tgcttgaaag aagcgtcttt tcacgagtct tggattcatt
    46801 gttttgagga tagctagaag tttatccaag acagtgggag aagaacagaa attcatgaaa
    46861 ggaatgtctc tttcttttgc tagtgggaga aaagtctcga aaagaaatct actaagttgg
    46921 ttttctgaaa tctttccact ttcactcccc actagagctg tatcacaaca attccaaacc
    46981 accatgaggt tcggacttct ttgattgtca acaaagatca ttcctttagt gttcctttcg
    47041 atcactgaaa gacccgttaa gctctgtgga agagtttcta ttaaggtctt atccttacta
    47101 atcatctcca gttcgctcct gagatgcaac ttgaactcct tctttctggg atcagtaaat
    47161 tctactccgt ttctgctaag caaagcaagg cagcgtgtgt agttctttgt tttgctattt
    47221 ataaatgtta gattatctca ggaggtggaa aattggagat caaaggatat gttgaaatcg
    47281 atggggttac gtacaaactg atcagtcgac agaagagtgc tcacaagaaa cttaggataa
    47341 gaagcaaggg ccagaccggt gttcaccttt tagaagagga gagctttctg aaagagaaca
    47401 gcgattattt gaagagtctc ttaaacgatt cggaagttgc cattccagtt ggaaaaaaca
    47461 gtgggggcca agatgaatga atttgcggat cagtatagag aaatagcttc cacaatgccc
    47521 tccgaggacg aaagaattgc ctgctacaac gaattccttc tcgacaggct tgagaatatt
    47581 atgccggggc ttcttgcgga agaggagttt gatgcctgga tggtaatcgg aagagagaac
    47641 aacgaagatc ctattatcag aacgttgctt cccggttcgt tttacggagc gagcagaata
    47701 acggccttct tgttcacgaa gaacaacaga tttgtattgt ctccacatgg tagtcagttg
    47761 agcggctatt atgaatccgc atggaaagtt gaagacggag atattttcga ttctatagga
    47821 aagctgttaa gtgatttgaa agtaaggaca ctaggagtag actattcgga caactttgca
    47881 ctcgccgatg ggattacggt ttcacttttc cagtctctca agagaaagct ttctgatgaa
    47941 gttactctgg tgagtgccga aaacattgca gtcaactggt tgcagaccag aagtgagaag
    48001 gagattgagg tttacaggaa tctcgacaga atagctcacg taataatcag aaatgccttt
    48061 tcgagagaca acattacacc gggagtcacg aaaaccagag atatcgagct cctgctaaaa
    48121 aagctggcat acgatattgg tctgaaaaca tggtttggcc cagatgtaga ttttcagaga
    48181 aagggcgtgg aggatgcgag gtgtacgggt ctaattgagg aaggtgacct attgcattgc
    48241 gattttggct ttatgtacaa cggcctcgca actgatacac agcaggtatt ctatctgaag
    48301 aaggaagggt atgacgattt gaagctaggt ctcgctgaag tcatcagaag aacaaacgag
    48361 gttcagaata ttctggcacg aaatttcagg gagggaatta ctggaaatga attgcttcga
    48421 gtttctcttg aagacgcgaa ggtaaagggt ctggaagcag caatttactc acatccagtt
    48481 ggttatcatg gacacggtgc ggggccagtt attggattat ggaacaacca agacagaata
    48541 agcggagccg gagatcttaa gatcagaaac atgacctgct ttgcaatgga gctaaattgc
    48601 agaacgcgac tgtcactgtg ggacgaacag aatgtctacg gtttcttgga agaaacggtt
    48661 gctttcaccg ataatgagat agactatcta gatggaaggc agaaagagct ttttctaata
    48721 taggaggtgt taaccatgaa gaaaacacta acggcgttct tttatattgc actgataatt
    48781 acattagggc ttgcgattga attgaaagag tcggctaact caatgagtgt aaatgatctt
    48841 gcgatacttg agattcaaga gaatccttcc acgggatatc tttggcatat ttttgcggaa
    48901 ccgacaggtg tgttgagaaa cttccttgaa gagcacaatg cgcccagtat gatgccgggc
    48961 gccccttctg tcaaagggtg gataatgaca gcggcaaagg aaggaaaggc tttgatcact
    49021 ttgaagctat tcaggcagtg ggaaggagag aaacactctg tggacttcag ggcactgacg
    49081 gtggatgttg ccggccaagg gaagggtcag gtggatctga agattctcat gaatgaagtg
    49141 acgcttggta cgaccttcac ggtcaccctt gaggagaatg ctagtactgg gtacacatgg
    49201 cagtatgtag ttcttggaga tgggattact gaaaaaagca aagagatctc agtcgataaa
    49261 agtgacaagg taggagctcc ttctaaggtt acctggacat ttcaagcggt ggacgaaggg
    49321 tattcgacga taatcttcag atacttcaga tcgtgggaag ggaaagagtc ggcggttgat
    49381 tataaagctt acaatatttc cgttgaatag ggtctgacat cattaaatga cgtttagttc
    49441 tgccgaggtg aaatactgtt gtgcattcca ggaagcagaa gcaagttgca ggtatacctg
    49501 acatcgattc tgatagtcgt tgtttcagca gtagtctttt ccctgcactc cattcagcca
    49561 gttaagtttt tcgccatact tagcgaaatg gttgttattg gtgtttcgct agtcgcagct
    49621 tacctactgg gaaatcgcga gctccacaaa gggatccctc tgggccttat ctttctatcg
    49681 atttcaacgt atttcgattt gcttgaagcg cttaccgaaa cgagagtgta tgaattcgtt
    49741 tcggtagtgc ttctggttat tggtttaccc atgctcctga taagtgcttt ctatcacatc
    49801 aaggagcgaa agaagagaat ctcagagttc agggccgttg taaacaattc actggacggc
    49861 attctgcttc tgggtctgtc aggagaaata ctccatccga acagggccgc ttgcagtttg
    49921 cttggatata gtagcgatga actcatgaaa aagcaagtat ctgatttgct ttccgaagat
    49981 agcgagaaga aattcgagaa ggcgaaatcc tccctttttg ctggaaagaa tgcctttcag
    50041 attgaactgg aactcattac cgcgagacaa ttcaagttag ttgctgaggt caatctagtg
    50101 attggaaggg atacgaaaga agatcccgat tcctttctca tgaccttcaa agatgtcact
    50161 cacctgcgaa agatcgagtc gactctcatt gaacagaaca agttccagag acttctcaac
    50221 gaattaatca cggaagtatt ggaaagcgat ttcgatgaaa agacctacag gtacttcttg
    50281 atgagatgtg ttgaggtctt cccaaaagca gatgcagcag cttttctttt gaaaaaggag
    50341 gataatcgtt atcatttcgt ggccactcac aactatgact ttgagaaact gaagacggtc
    50401 tcgttttctt ctgatgaact tattcaaaaa ggtagtggtg atgtggttat aataaaggat
    50461 tacagtgttg attatcaaat ggatggtgag cgactggaga agattataaa gggaggaagg
    50521 cttgaggaaa taaagagcac actttcgatc ccaataaaga ttgatgatga catcagaatg
    50581 tacttcaatc tggacagctt cacttcttca actgctttcg atgacaaaga ggtagtaaac
    50641 acggcaattg gttttggaaa agcgatttca gttcttctat ggagaattca gacagtaaat
    50701 gaactgagaa gacagagaaa gctgatggaa aagctatctt tggaggatca cctgaccggt
    50761 cttccgaatc gcagagcctt ctttgattgc gcggaaaggc aggttgagtt ttcgaaacga
    50821 aagtctgagg caatggctct tctttatctc gatctaaatg atttcaaagg agtcaacgat
    50881 actcttggac atgatgttgg tgatgagctt ttaaaaagtg ttggcaagcg ccttagtacc
    50941 gtctgccgta gaagtgatct cattgcaagg atgggcgggg acgaatttgt gtacgtacta
    51001 ccttcaacgg gaaagaaagg gtctagagaa gcggaaacga gaatccatca agcctttgaa
    51061 gacccctttt ctgtcaaggg aagaagcttg aaactgactg ccagtgtcgg tatagcagta
    51121 tttcctgatg acgggaagac gattagagaa ttgctgatcg tagccgacga aaagatgtat
    51181 gaaaacaagg agaagctctc aagagagtca aagagggtga ctattacctg at
//
LOCUS       DAOQYI010000012        51171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_739_length_51171_cov_72.548850, whole genome shotgun sequence.
ACCESSION   DAOQYI010000012 DAOQYI010000000
VERSION     DAOQYI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 51171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51171
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_739_length_51171_cov_72.548850"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PKL93_05175"
     CDS             <1..267
                     /locus_tag="PKL93_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70687.1"
                     /translation="AILPDFGVLELLSTNGSISIFLDDSIRAEISASTTNGTINLKEL
                     TVGVISSSSTSLRGSMNGGNGLSINMKTTNSNITIDKHNSEDSI"
     gene            complement(792..2582)
                     /locus_tag="PKL93_05180"
     CDS             complement(792..2582)
                     /locus_tag="PKL93_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HNQ70688.1"
                     /translation="MNWKKGIPAVVIVLILAALIPAMVFGKTSIHELVVMGTTDLHGY
                     VMPYDYLTVEEVSDYGAAKTFTLIKMAREMYRNTVLIDTGDAIQGSVLAEREARIDPI
                     KMGDTPSIIKAMNIMDYDVVGIGNHEFNFGLEYLDLAIASADFPMISANLYNEETQEL
                     RYDPYVILEREVDGIPIKIGVIAFLPPEIMMWDRVLLEGKVYAEPIVDAAAKYVPKLR
                     EEGVDLVIVSLHQGSSDAEAILENVEGIDAIIMGHSHGKIADKIDGVPVTMAGSWGSS
                     LGLIHFHLLNRDGEWEIFSSCPQLWHVDEEVEAAPEIVEAVKEQHEATIDYVMAPVGA
                     TTVPIKGYFSRVIDNEVTQLVNEAQLWYVKEYFKGTEYESMPLLSAAAPFRTNTSVDA
                     GDVRIMNAADIYIYSNTLHVVKVNGLELKGWLEKSAENFNQIDPNSTEEQNLLARFSS
                     YNFDVIEGIDYQIDVRNPVGQRIVNLTYEGEEIEDDMEFLVVTNNYRAGGGGYHLVDA
                     DIVLSSTVENRSVIIDYIIEKGTINPTPSYNWSIASFESAGRITFTSNQEAASLIDEL
                     GIKGIKYIGDRLFEVDLIELAENVPLTVSN"
     gene            complement(4210..5724)
                     /locus_tag="PKL93_05185"
     CDS             complement(4210..5724)
                     /locus_tag="PKL93_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ70689.1"
                     /translation="MRIAISVAELAVTWIIIPFLLFSGAPFSAGLGVTIVASIIIILS
                     LLLAIYSALVVYYWSGRLPTVIYGPEKTVQSGPYRFVRHPFNLGYILFLFGLGLLSGN
                     YWTLLYVAVIGAVIVVYSLLQEKLALKRIEGYEDYKEKIPFMIPNPGRKIPFDKSTSI
                     PWQFIVASFVVKLVILIVLPSKVKNARVLREKRPFVLALAHQTHFDGPLIFYSTWRYL
                     RFVGTAIYVDRLGLLNWLAVIPVRRYAVDTYAIRQMLSTIKQGVPLGIAPEAARSWDG
                     RPLHTKREIWKLFRMLKIPVIPVKFYGVQRLWPRWAKIFALGTSTVEFGDPVEADDPQ
                     LEEKVMGFLAKEDPTFKLPYRNYKRIEKLIWRCPSCGAVASIKGFKTGFSCSSCGKSW
                     TKPTVNEVIQIHDRIMPGSMGLSFPIKDEVIFNGERVTANIYEDHAMIGDYKLDYSVI
                     NNSSIEKSIEPVFGIANEMVSFVSTTSALMWQELVDFQIKFRLKKENYHTDLWG"
     gene            complement(5721..6998)
                     /locus_tag="PKL93_05190"
     CDS             complement(5721..6998)
                     /locus_tag="PKL93_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ70690.1"
                     /translation="MSDRRKLSTATQVAIYSVVLIVSILFASAIFVYGVSESVFIRNY
                     SAEMRKIFMDSPMGKGFRYGVGRMAIGRGTTEFYVSVDGNVVQNPTGLSSVPDVEGYV
                     KTRIDGSSYLLYGFLNGEEKVILGARMDDLDFFLESLATTLLLSVLVGVVLSIIGGFF
                     LGSRVSKPIKETSRLLKEITLNDLSRRLESDPKTSELAELKKALNIALDRIQDGYTRQ
                     EQFSSDIAHEIRSPLTSILGFSRLILRWGSDDPEVVKEAAESISDTATKMMTITESLL
                     FLSRPEIELNETSFNLRELVLEITNSVPADQSAEMKIEIPDIEVRTDRVLLKIIVKIL
                     VENALKFGKNGPIEINWSDGTLSVRDHGMGINEDEKERIFNRFYRGDSSRGGEGHGLG
                     LSIASKICKVLKLSIDADNCEDGGAVFKVEGLR"
     gene            complement(6988..7650)
                     /locus_tag="PKL93_05195"
     CDS             complement(6988..7650)
                     /locus_tag="PKL93_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ70691.1"
                     /translation="MKGRIMVVEDDMHIGKLLRLELGHEGYEVEVLTDGKEALKHLEK
                     ELPDLLILDVMLPGLDGFSVLEEIREYISTDLPVIMLTAKGEVKDRIRGLKSGADDYL
                     PKPFVIEELLARIEAVLRRKGKVERISYFEITLDMQSREAFLNGEPLQLSKTEFDLLA
                     VLLSNAGIVMSKERLLEKVWGSEDWGNPNVVEVYINYLRKKLGKTGERIKTVRGSGYV
                     VR"
     gene            complement(7647..8528)
                     /locus_tag="PKL93_05200"
     CDS             complement(7647..8528)
                     /locus_tag="PKL93_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ70692.1"
                     /translation="MKLKSKAYVNLLLVVIFWGLTFPVQKNILEGLSPVFYNALRFSI
                     ALLVLIPLRKKLGLKITNKSLTQGLILGLFLSGGYVFQTWGLVYTTASKSAFITSLYV
                     GLVAIIGPVVDRRIPSRYQILALGVSLIGLYFLTTPETGFNFGDLLTALCAVSFALHV
                     VLISYFTQKEDVDEMELLVPQFVVVLLVNMALIPFVKGSVFINGSILFVALFSAVFAT
                     IFAVAVQLKYQRFLGSVGASLIYVGEPAFALFFAMIILREIPGKMEVVGLLLMTAGMV
                     LGGISSFVMRDRSVKKG"
     gene            complement(8560..9633)
                     /locus_tag="PKL93_05205"
     CDS             complement(8560..9633)
                     /locus_tag="PKL93_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNQ70693.1"
                     /translation="MKKALLLAIVFLSVSLFATSVSYDPQGLMFLSRPGASINVNVSL
                     NKGMGSTYYGGESLGLSFSVDRSAYVAVLDIDPSGQIQVLFPNVYDQDNYLQGGRTYT
                     LPTDKATTRYNLQVQSQRGRETIVVVASTSPLNFLGNVFSLFDRYPFPYLSQSINNLS
                     VVIDPVFNTSWGLGYTYFYNSYVPFTVRTTINADRKDANVYVDGIFMGKSPVTTTLEA
                     GPHTVYVYTERNLVYGPATINVQPGSSDFYFSLLPNYVYGYLEVTSIPDGQVYVDGQY
                     AGDTPYKDFEKVGSHTVTVSKWGYHDSKQNVYINKDMVTSVSFTLTEKTEEEKKTDNI
                     ILFSIIGVLIAGVVIAVILGASD"
     gene            complement(9630..10577)
                     /locus_tag="PKL93_05210"
     CDS             complement(9630..10577)
                     /locus_tag="PKL93_05210"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNQ70694.1"
                     /translation="MKVTIVGAGMVGSSIAYASMIKGVAREIALVDINRELAKGQAMD
                     LSHGNAYVRPMRIHGGDYDVSSDSDVVVITAGRPQKGGESRLQLLKDNARIVKEAVAK
                     SLSYSKEPVFIIVTNPVDVLTWVAWKASGLPRNRIVGSGTTLDTARLRQSIADHCQLD
                     PRNVHAYVLGEHGDSEIVNWSHADIAGIPLTDFCRNCRRDCEADLFQKLFEETKNAAY
                     KIIEKKGSTFYGIGLAVSKVLSTIFNNQHSVLTVSSVHPEFEGLKDVPFSVPTILGRE
                     GVERILKVGLSSAELKGLSNSAGIIANAIKNLRDEEGLA"
     gene            complement(10577..11617)
                     /gene="ispG"
                     /locus_tag="PKL93_05215"
                     /gene_synonym="gcpE"
     CDS             complement(10577..11617)
                     /gene="ispG"
                     /locus_tag="PKL93_05215"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNQ70695.1"
                     /translation="MSGVVRVGDVLIGGGNPITVQSMTNTKTSDVNKTVEQIKRLEDA
                     GCDLVRVSVPDFESASVLKEITSLSRIPVIADIHFDYKLAVESIKNGAAKVRINPGNI
                     GQDWKVKETVKIAKEYGVPIRVGANSGSLDKRFSSYEKPIALAESALAQVKILESAGF
                     EDIVISLKSSSVIETVEANKYLYNKTDHPLHLGVTEAGFGLDSTVKSSIGIGTLLLNK
                     IGDTIRVSMSGDPVQEIEVGLSILRSLKLRRGVDVISCPTCARTEIDVEALARKVKSW
                     VGNTDADLSVAVMGCVVNGIGEGTEADIGIAGTASGGVIFVKGEIVEKVERADLEARF
                     RHWFKTVLSGGV"
     gene            complement(11614..13125)
                     /locus_tag="PKL93_05220"
     CDS             complement(11614..13125)
                     /locus_tag="PKL93_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNQ70696.1"
                     /translation="MLLAIIYFLLILTGIVVVHELGHYIFSRLFGVRVLEFAIGMGPK
                     LWSKKGKNTTFRINAFPIGGYVRPAGEDLDTIDSSIPESEQIQNKPAWQRFIIYFAGP
                     AFSLLLGFLILSLVAVIWGFQEVKIDKVEPGSPAAVSGMMPGDRIVSVNGKTLIDNTR
                     LSEAVAKGERIDLVVNREGKEIEIAIIPELLPQEAFFVIGGVSGEPGDILSSINGGTF
                     NGDYVGYGSVLSPESSIKLGFEDGSTLSGTLLTYSPAEERYAMGIYYANFKPQLAIDH
                     GQFREGDILLSVNGMKTETSYDLTTMSQLLALKPDELLIQFTGKEISFAEFGFPDEFV
                     VSVEREGQLINLTVSKNEMIALVEEAGAFAQGSSYWYPDTALEAVGLGVQWANNLLIA
                     LVKVVGSLFTGGANINEFTGPIGLVTIVDQAVSLGLRIVLYLAGFISLNLGVINLIPF
                     PALDGGRMLLALIEMITRRRLDPKIEGLINVIGFMVLMGFMIYITFIDIGRWL"
     gene            complement(13116..14246)
                     /gene="dxr"
                     /locus_tag="PKL93_05225"
     CDS             complement(13116..14246)
                     /gene="dxr"
                     /locus_tag="PKL93_05225"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNQ70697.1"
                     /translation="MKKRLAILGSTGSIGSQTLQIANRIENIEIVAISCGHNLSLFSK
                     QLSEFHPPFAASLKKSDSLTDSFPATLFFHGEEGIERMLEESKPDYVLLAVSGAAGLR
                     FSLKAIEVCHRLCLANKESIVCGGDLLLKECARKGVELIPVDSEHSGLFQLLDGSRRP
                     KRIIITASGGALRDWPLERIQDATIRDVLRHPVWSMGNRITIDSASMVNKGLEVIEAK
                     YLFEFETNEIDTYICRNSFIHAGILYSDGVLKLHTGIPDMRIPIAYSLTYPERIQTIP
                     EQSIVDVPMILEELPKERFPALTLAREICGVVSKQIAYNAADEVAVEAFMNGRMPFGG
                     IYNIIEKTVERTADQNPVDYSQIIETDSVARKLAQEEVDRCY"
     gene            complement(14243..14935)
                     /locus_tag="PKL93_05230"
     CDS             complement(14243..14935)
                     /locus_tag="PKL93_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNQ70698.1"
                     /translation="MTIVYSVLFMSVLGVGAGIFLAFASAKFAVKKDPRIALIEASLP
                     GANCGACGFPGCAAFAKAVAEGKAPIEGCIPGKRSGVPEKLKLILDTDIDKLIALFDE
                     NEEDAEKTLEKLLASSGKAVKAAPPKIQRPTQEEIDSYKEKLKENPRAAVIFAVLPNI
                     NCALCGSPGCAAFAIKVANKDEDIAKCVPGKPQNVSQKVAKIMALSEAELQKIIEETS
                     GEPAEIKKKFAS"
     gene            complement(14946..15527)
                     /gene="rsxA"
                     /locus_tag="PKL93_05235"
     CDS             complement(14946..15527)
                     /gene="rsxA"
                     /locus_tag="PKL93_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNQ70699.1"
                     /translation="MATRLVFIFLSAILINNFVLSRFLGICPFLGVSRKTDTAVGMSI
                     AVTFVMILASIITWILNIALDALGLPFLRTIVFILVIATLVQFVEIFLKKASPGLYEA
                     LGIFLPLITTNCAILGLALLNVQQNYTLVETIVNSAGAGLGFALALILFSTIRERMEL
                     SEIPEPFKGTAIALITAGLLSMAFMGFQGLVKL"
     gene            complement(15541..16155)
                     /locus_tag="PKL93_05240"
     CDS             complement(15541..16155)
                     /locus_tag="PKL93_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNQ70700.1"
                     /translation="MMAESKFKILANGILKQNPTFIQVLGMCPTLATTTSAENGLGMG
                     LATTAVLALSNITISLVRKFIPDKIRIPSYIVIIASFVTVIDMLMHGFVYDLWKTLGL
                     FIPLIVVNCIILGRAEAFASKNDVFSSFLDGLGMGLGFTASLVLLGSVREFLGNGSIF
                     GYHLSDVKMFAMILPPGAFIALGMLAAFFNYLGIRRSKVKKAEK"
     gene            complement(16152..16874)
                     /locus_tag="PKL93_05245"
     CDS             complement(16152..16874)
                     /locus_tag="PKL93_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNQ70701.1"
                     /translation="MRDYLKTGITLMVITIIAALVLSVVYTIVEEPIANAELGAKLKA
                     IREVLTDSDTGELLIDEESIPQTATELAKFEWLPQGFSPSEGIIYRTEDNQGKVESPA
                     YKFVKEDGTEIFIAIGIAVGYGGDVKSMAAFVKEPDGVKLNGIKVLEYSQETPGLGAN
                     IANADVQKRFYPVSKEGLDKGIRVDKDAGVTPVGDQIDARKREDGIVTVSDVMTGATI
                     TPRAVASSLNAISEFLDKAGVR"
     gene            complement(16867..17817)
                     /locus_tag="PKL93_05250"
     CDS             complement(16867..17817)
                     /locus_tag="PKL93_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNQ70702.1"
                     /translation="MKLSMMAAPHLRSEDSVRKIMLDVLIALAPAAVWAVVSFGLKAL
                     VLIVVCTAGAEVLDFFVMRFIRRKKNFKPDLSGSVTGLLLALNLSVAVEWWQALIGVF
                     VAIVIAKAVFGGLGKNFFNPALVGRVFLLISFPVQMTTWLSPFDMQTTATPMAILKQG
                     GPRVFSLQEMFLGTIPGSLGEVSALLLLLGFGWLLFRKRVSPFIPVAYIGTVVIMAAI
                     FNGVNPGFGSPLYHVFGGGLMLGALFMATDMVTSPMSIKGHVIFGVGCGAVTMIIRLF
                     GGYPEGVSFAILVMNAFVPLIDMGTKPRIFGKPKKVKSNA"
     gene            complement(17814..19133)
                     /gene="rsxC"
                     /locus_tag="PKL93_05255"
     CDS             complement(17814..19133)
                     /gene="rsxC"
                     /locus_tag="PKL93_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNQ70703.1"
                     /translation="MRLPTFRGGVHPPEKKELSKDSRLSVLPLPERVYVFLANHAGVP
                     AKPLVNVGDSVKTGQLIAEASGFISANLHSPLTGEVKEIAKYYHPTLSKPDDAIVIEK
                     SGEEEWELLEPAKPYEQFEPEEIIQRIKDAGIVGLGGAMFPTNVKLSPPKEKKIDLLI
                     LNGAECEPYLTIDYRYMLERSEGIVRGILAIMRALKVDNAVIGIEDNKQKAIEQMEVA
                     ARSTPIQVKRLKTKYPQGAEKQLIYALTKRKVPSGGLPLDVGVVVDNIGTAFAVYEAV
                     ELGHPLVERGVTLTGEGIKKPLNVISRIGVMASELIEYAGGAQEIVERIVFGGPMMGI
                     TVPKIDVPTVKGTSGITLLMKSDPLEEFPCIRCGKCATVCPMNLQPFLLNLYGTNRLY
                     DKQVENGLLDCIECGSCSYVCPANIELVKNFKLNKKVYRSLKGGAKK"
     gene            complement(19299..20756)
                     /locus_tag="PKL93_05260"
     CDS             complement(19299..20756)
                     /locus_tag="PKL93_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ70704.1"
                     /translation="MKKVLLITLLLIMAVFVMGQKLTILHINDTHGHIWPEGDYGGLA
                     AITTLVNQVRAENPNTLFLHAGDINTGVPESDLQDAAPDIVALNLMKLDAMAIGNHEF
                     DNDASVLAKQMEIANFPFLSANIYKDGEPAFQEYMITEVGGIKVAIVGFTTQETEILE
                     YLYAKDYEWKDVIEVAKEIIPELEAQADIVIALTHMGSNPVLAGPNSWELAKAVDGID
                     VIVDGHSHTFYERPEIINETIIVSAGEWAKNLGKLELEIVDDTVIFVSFRNLPVIAEE
                     IEPDFAVATVLDYFKKAGGEALNIVVGETAIRLEGDRGVVRSQDTNLGYLICDAMVWK
                     TGADLALTNSGGIRASIEAGPITYRDILTVLPFGNTLYVLEVPGTALREILQYTATRE
                     AGQGAMAQVSGVTYVIENKEAKDILVNGEPIDDNKLYKLATNNYLASGGDGYEILAGL
                     SGYDTGFVLADVVVEFVGEISPITSYPDSGRITRK"
     gene            complement(20770..21120)
                     /locus_tag="PKL93_05265"
     CDS             complement(20770..21120)
                     /locus_tag="PKL93_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70705.1"
                     /translation="MLKAVKPFPPKRKGVASFTLLIAILIVCTAMVTVLAPVDTIMVS
                     RRLSSWYFRLALTIKSIYLAEPEETEINGLQMEKSSRTVYSNCAKIEIINYHIIEGEK
                     SFDFEIVGNITDIS"
     gene            complement(21083..22351)
                     /locus_tag="PKL93_05270"
     CDS             complement(21083..22351)
                     /locus_tag="PKL93_05270"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNQ70706.1"
                     /translation="MAKKVVALILAGGQGTRLGLLTDEVAKPAVPFGGKYRIIDFSLS
                     NCVNSGITAVGVLTQYRPHILSRHIGIGRPWDLDRKDGGVVILPPFIAQGDSNWYKGT
                     ANAVFQNIDFVDDFDPELLVILSGDHIYSMDYNEMIDYHLSKGATGTVACMRVPLSEA
                     HRFGMMITDFENKITDFQEKPKEPKSDLASLGIYVFDWKFLRERLIQDEKDPESDNDF
                     GKNILPKIVQDNAGKLFAFVFEGYWRDVGTIESLWEANIELTRPIPPFNLHDPSWRFY
                     TQTEEYLPAYVGTSSYVRNSLINEGAEIYGRVENSVIFQGVQIGEGSLVKNSVVMTNS
                     KIGRNVVIERGIVGEGTFVKDGVVLGYGDEVPHETQPHIYNAGIVVVGSDVVIPQNTK
                     IGKNSAILNHVREEDFEGDVEGGKTISPKA"
     gene            complement(22365..23492)
                     /gene="glgD"
                     /locus_tag="PKL93_05275"
     CDS             complement(22365..23492)
                     /gene="glgD"
                     /locus_tag="PKL93_05275"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HNQ70707.1"
                     /translation="MKVLGIILAGGRGEYLTPLTQVRASAALPVYGKYRSIDFTLSNM
                     VNAGISKVGIITQYSPRSLMDHIGSGKEWDLDRKQGGLFILQPYYSPDNPSMGYKGTA
                     DALFQNINILRRGNEDSVLIGSGDHIFKTDLAKIFRYHLDTAADITVLTGNKEGECGM
                     EGMDRVVCRNGRVVKWIEKEKIAENPQEGDCVALDIYFINKFLLRELLYSAVPNGENE
                     LVKGIISPNLSSLNVREYRYNGYWRDIKLSKKCYFRTNLDILNPEIRRELFYENGRVF
                     TKLKDLPPPKITGTAYMNNSVIADGCVIGGRIEDSVLFRDTRVMAGATVKNSVLLEGC
                     LVEEGAYIENVIMDKYCTVRLGRSFVGEAEDPTVIEKHGVI"
     gene            complement(23552..24367)
                     /locus_tag="PKL93_05280"
     CDS             complement(23552..24367)
                     /locus_tag="PKL93_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNQ70708.1"
                     /translation="MPSFWTHIMYGKDVLEEIGLDLEADKREEFNLGCLFTDPGLYCS
                     KDDPEFSLWRFSHTIRCDEFAIALCERAGGISRFYTLGVICHYFLDATVNHYIVARAG
                     NGYKYRQLETMIDKVILKDRIEADILDLDPKAEFSGSLPAALEDLYRDISLEFYGLKG
                     YSFQRAVESMNNYFEGLYGRSRLALILKRFSIREGSPEEAFFEDTHPDPLNLAMKPWF
                     HSFAGWESKKTFHELYKEAFDNAVHFLQNYLDGVGDFSLPRVSLMTNLPLMEY"
     gene            complement(24374..25552)
                     /locus_tag="PKL93_05285"
     CDS             complement(24374..25552)
                     /locus_tag="PKL93_05285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNQ70709.1"
                     /translation="MLIARIKKGKEGKVLNSYPWIFKDEIMSLEGPTDKVCEVNVFSA
                     GYEFLGKGFFSPFSSRAIMVLTNKDEELGEGFFGRLLSNALRKRERLFSRPFYRLVHG
                     EGDRLPGLVIDRYGEIIAIQFRNLIIQQKREMILRLLRDIVKPNGIYERSDFEMGVDE
                     KIERHTGLIFGEVPDSIEIEENGLKYLVDVKNSQKTGFFYDQRDSRTFCRKITEELSL
                     KKGLDLFSFTGGFGLNMACSGADAICVDKSGDDLEQGRMNSVVNKLDNKLQFVQSDVL
                     DFLAGFEKKDYFDIVVVDPPSFVKHRKELPHGISLFKRLVEGTLPLVKDGGVLGLCTC
                     AYNIGIDHLVESVRRSTERTGITFSHLTITLQSPDHPWLVEVPESLYLKCLWGLVEKE
                     "
     gene            complement(25552..25995)
                     /gene="ndk"
                     /locus_tag="PKL93_05290"
     CDS             complement(25552..25995)
                     /gene="ndk"
                     /locus_tag="PKL93_05290"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNQ70710.1"
                     /translation="MERTFAYLKPNSIQRGLVGEIIRRIEEKGFKIVALKMLRISESR
                     ARELYREHAGKEFYEPLLAFVQSGPVVAMILEGEDAVKRLRVLVGKTDPTEAYPGSIR
                     GSFGVSVRKNLIHASDSVESAEREARIFFDESEINDYSLVIEGQL"
     gene            26121..27071
                     /locus_tag="PKL93_05295"
     CDS             26121..27071
                     /locus_tag="PKL93_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70711.1"
                     /translation="MGRDSEYLKVLATSIVEGDPKWSKRRYVEDFLLIEPESGNIGID
                     RIRDIEEIMLHRPSGDGKKYVLIHQCERMTDESANAFLKILEEPPSFATLIMTTTSWQ
                     SLLPTIRSRTVSMRGEVPRELMDELRAKYGKRVPEIFAASRENFTILRYFLEQNPDSV
                     ESASFEVPSDLDGLVELIKEDVSESFESIVRKRRAFVALSKNFISTDSFFSTFRRVSS
                     VFTGSGSFERARELVKVVRSILSDAVIASVNPSSKSVTNLDLIEWLVDFDLGKEIMTD
                     FIWCDRFLRQRTSSLSSTLVALRALNSLSRNLLRRENGGN"
     gene            27073..27957
                     /gene="ricT"
                     /locus_tag="PKL93_05300"
     CDS             27073..27957
                     /gene="ricT"
                     /locus_tag="PKL93_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNQ70712.1"
                     /translation="MPEIYGTVYGVEFHPVGKLYQYTSSEGLLKQGDYVLAISEFGLD
                     VGKVMYGPVEIRIDDSKEELKPIVRVMTDEDWETDSKNKEEAKEAMKTCQELIEKHSL
                     PMRLLEARYMFDRSRIVFYFGADSRVDFRELVKDLARAFRTRIELRQVGIRDEVKMKG
                     SLGLCGMTACCVRFLRQFESITLKHAKKQQLLINPAKISGRCGRLLCCLSYEQELYEN
                     ELKDIPDEGSLVDYEGKTCKVLTVNIFMKIITLVSDDGQMLKVQFEDFRKSQRAIIQD
                     TNPDELIKNRDEDISIDD"
     gene            27978..29876
                     /locus_tag="PKL93_05305"
     CDS             27978..29876
                     /locus_tag="PKL93_05305"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNQ70713.1"
                     /translation="MAGLRTREELKRLEDLGRICRGDIIKMTTVANSGHPGGSMSSID
                     IFLSIYDFAKNDPNKPFDPERDRVIVSHGHTSPGVYSTLGRLGFVDIDEVIAGFRHPG
                     SIFEGHITRGIPGVEWTTGNLGQGLSVGVGMALASKLSGKNNRVFVAMSDAEQAKGQV
                     GEARRTAVKCGLDNLVVVIDYNDAQISGRAHNIMYVDIKANYLADGWKVIEVDGHDYL
                     ELNEALVEAEDSKGPVVVIGKTIMGKGVSFMEGDVSYHGKPLDFEKAALALAELGLEN
                     DIDRFVQMRKVLPSYHEPVQAIDEDVKPFTGDPIVYPVEKKTDNRSAFGKAVGDIGRL
                     NKGKLPVMVVDCDLKPSTKVSEFEKVWPENFVQIGVQEHNAATLAGAASVCGVLTFFA
                     DFGVFGVDETYNQQRLNDINKANVKVGVTHVGIDVGEDGKTHHCLDYIGALRNFYGFK
                     LIVPADPNQTDKAVRFMSETKGNFVIAMGRSTMNPVADENGNPFFGEEYKFEYGKVDV
                     IRKGKDITLVTTGQVTHHAVKAADELRSHGIEVAVLNVSCPLGANFESVKEFFSSIVI
                     SLEDHNVASGLGSILADYLVGSGIIPKSFEKIGVERYMFSGDNELLYDLAGLSSKAIV
                     ERIRGIIK"
     gene            29876..30601
                     /gene="rnc"
                     /locus_tag="PKL93_05310"
     CDS             29876..30601
                     /gene="rnc"
                     /locus_tag="PKL93_05310"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNQ70714.1"
                     /translation="MLSAEEEKLIEKFCRINGIEADRELVFRALCHSSFTNELAQNGE
                     RTLESNERMEFLGDAVLELSLAKTLYNEYYLTEGEMSKIRAMVGSEKVLSSVAKTMRI
                     GDFIFLGKGERQTGGAERDSILADAFEAVLAAIFLTGGLGVSVEFVQSKLSDYIERAV
                     EGELILDYKTSLQELTQARFGSRPLYETLLDEGPPQEKWFKVGVFLDGSLMGEGEGRT
                     KKAAEQLAAKHALEILKKSGAKK"
     gene            30598..31410
                     /locus_tag="PKL93_05315"
     CDS             30598..31410
                     /locus_tag="PKL93_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNQ70715.1"
                     /translation="MRYRHLIAFVTPEESDSIEGMSWNEGFTNLYFEEIEGSGISLHV
                     LLEEGEEVPAFLREIEFSDLGFSEERDWFQKWKETLVPFNLCDGFEVIPLEEEKMVGS
                     GKIGIIPGMAFGTGLHESTRLAAAILNETVREGLKVLDVGCGTGILSVIAARKGAAAV
                     VALDIDEHSIEKTIETARINEVSIEARLSDFLSAVKPGERFDLIVSNMIAELLMKFVL
                     DLPKFLTEDGMVILSGIYKDKYREIESLAEEEFSIENVREDGDWKAITLKRK"
     gene            31380..31913
                     /locus_tag="PKL93_05320"
     CDS             31380..31913
                     /locus_tag="PKL93_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HNQ70716.1"
                     /translation="MESNNIEKKIVSLQLGRAVKNDFIVARLCDWGFPQVIESSLISD
                     GRPFPTLFWLTCPFLKGEVSRLESKGMISHLEKRIEQDKSFATAFLGAHRETTLLKEK
                     ILCEASVSGESRRLLLDRGIGGIRNLKKVKCLHLHLANYLGGIDNPVGREVWNLLEKK
                     QCSSTKIICRELLRENE"
     gene            31906..33633
                     /gene="uvrC"
                     /locus_tag="PKL93_05325"
     CDS             31906..33633
                     /gene="uvrC"
                     /locus_tag="PKL93_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNQ70717.1"
                     /translation="MNEAILNKASQLPEEPGVYIFKNEKDDVVYVGKAKKLKRRVLSY
                     FRESTWSKNEKARCIAEEAEDLDFIMVTSEREALLLEANLIFNKKPKFNVLLRDSRTY
                     PYIYISADQYPYLAITRTKELEGSYFGPYTSAGLVRKLLEFLQKVFKIRTCTYELSRV
                     KKPCFLYHLKMCSAPCVEKVSPEEYREQLSALIEFLEGDTLKVREALLKRMAVLSEAL
                     QFERAAEIRDILSTMDDLYAFQGVEAPLDLKADVLAVSAGLAALLQVRGGMLLGKLIF
                     DFPEGTPIDFITQFYYAKGNRMPKALIVTGLKKKDIKQFKRDFPYIGEPRDEQEERLL
                     SIAFKNIDEEMKIRLNATHSLKQAQQILGLKRFPSRIEGIDISHTQGLYTVASVVVFD
                     NGKPNKSEYRRYRISELEEPNDFEAMATVVKRRYTKHPLPDLLLIDGGEPQLRAVEKA
                     FAEIGIQEYEIVGLAKEFNELVFLDNRDRVKLKEEHPVLRMIISIDDEAHRFAINYHR
                     VLRERRFQSSKIDDIPGIGPKRKKALMRAFGSVKGIAEASEADLYSVLRNSKAVEAVT
                     KWANEKSGD"
     gene            complement(33666..33938)
                     /locus_tag="PKL93_05330"
     CDS             complement(33666..33938)
                     /locus_tag="PKL93_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ70718.1"
                     /translation="MNKKELIAVLAEKTGATKKLVGDVIDGFIDVVGEELSKNEEVKL
                     VGFGTFEVTKRKARKGVNPRTKEAIEIPGGKVPKFRPGKELKEKVQ"
     gene            complement(34050..35393)
                     /locus_tag="PKL93_05335"
     CDS             complement(34050..35393)
                     /locus_tag="PKL93_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNQ70719.1"
                     /translation="MKKRTVVNLKSEELPRSWYNIKADLPFKMDPPLNPATNEVINPE
                     DMMAIFPKSIVEQEMSEERFIPIPEPVLDEYAVFRPSPLIRATFLEEYLNTPARIYYK
                     YEGNSPTGSHKTNTAIAQAYYNKTDGITELTTETGAGQWGSALSYAGRKFGLTVRVFM
                     VRNSFQSKPMRQHMMRMFDGTVEPSPGENTESGRNFLKDRKNFSGSLGMAISEAIEIV
                     LNSKTKKYSLGSVLDHVLLHQTVIGLEVEKQLEMLGEKPSSIIGCHGGGSNFGGTILP
                     FVKRTFNGDDIEFIACEPESCPSLSKGEYRYDSGDSAGFTPLLKMYTLGREFVPPAIH
                     AGGLRYHGAAPLISRLVKEGIVTPIAFPQEETFRAGLLFATTEGIIPAPESCHAIAGA
                     VRKAIEARETGEEKVIVFTLSGNGALDLSSFTSTLEAHRDVLNFDKLRESSIVEQ"
     gene            35936..36826
                     /locus_tag="PKL93_05340"
     CDS             35936..36826
                     /locus_tag="PKL93_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNQ70720.1"
                     /translation="MKNFVSAAIQFEAEPMNVKVNLQKAYHLIGNCKRETGASLVVLP
                     ESFTTGFTPIGNAESLWEVVDSIPGSLTDEAVRWAHEFDTYICFPTYERGKERAVIYN
                     SAAIVGPEGMIGVYRKTHPFPTERLSAGGWTTPGSDPLVVSASIGKIGVVICYDGDFP
                     ELARVTALKGAEVICRPSAFLRTFDQWELTNRARAYDNHVYWIASNLVGKDSSGSNFF
                     GSSMIVHPSGMKLAQASGCEEFVSAMLEADPIRKITPGASRDQIFDHIEDRNLDSYKG
                     ILSEGKSAFQPSKRIPYRRR"
     gene            36829..38013
                     /locus_tag="PKL93_05345"
     CDS             36829..38013
                     /locus_tag="PKL93_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNQ70721.1"
                     /translation="MSKPLEGISVLDLTRVLAGPFCTMLLCDMGANVVKVERPEGGDD
                     SRSFSPFVGDDSAYFMSINRGKKSITLNLKTVEGKKLLERLIERFDVLVENFRPGVFE
                     RLGFSAQRLREINPRLIYTCSSGFGHSGPMSHRTAYDLIIQGLSGMMSITGPDENSPT
                     KVGSSIADIFCGTFACIGILAALHSRNLTGKGQKVDVAMLDSMVSVLENAIARYAATG
                     EPPVPIGNRHPSIAPFASFKTLDGIINIAVGNDVLWEKFCKAIGRPEMCDDPKFVNNP
                     ARVRNLEELMEILGGELEKRTSDEWLGVFGKLDIPSGKINNIADIFEDEQIRAREMIV
                     EVPHRCGNVKMPGVPIKFSDTPASIAGPAPLLGEHNREVYAGMLGLSDLEMEELKRKG
                     II"
     gene            38010..38717
                     /locus_tag="PKL93_05350"
     CDS             38010..38717
                     /locus_tag="PKL93_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HNQ70722.1"
                     /translation="MIYLYPYERSSDWQESTYRLTVKTAFSKSVNFRDEQGRRYSLLV
                     DDDDFLPRSALIEGLPPMASGHEVGIDIKGAVVKFDPSAMSGLPDRKMRGLWLEWLSL
                     MDNAELKCIQENLDEPFRLVGLGPGLTPAGDDFLVGWITACKLEGRNTKKLSSEIEEA
                     LSRKTTWFSSEMIRDALDGKFWKRGIELALALNEGDSFQVMKRAGKIINWGHSSGKAW
                     LAGFAYGIERGSDPQLI"
     gene            38839..40371
                     /locus_tag="PKL93_05355"
     CDS             38839..40371
                     /locus_tag="PKL93_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid metabolism regulator protein"
                     /protein_id="HNQ70723.1"
                     /translation="MKKIEVVRGEYYDSVTLMLVAKEIKRINGVVDAVLNMATEANID
                     IMKAAGFEIKAGFLSPDDLLIGIDYRKDEIDKIFETARSYLASPPWKKKEDDTEYTPG
                     TLEGALAVLRDANLALISLPGRFAAAEAMKALKNGLNVMLYSDNVATEDEIKLKHYAD
                     RNDLIVMGPDCGTAVINGKGLGFSNVCPEGPVGIVAASGTGLQEVMVQLSRRGIGVKH
                     GIGTGGRDVKECVGGISFLKGIRELSDDPEISLIIAVGKPPDPEVKERILNSLKKTGK
                     DCVICFMGDDCGKDEDRVHYSAELEECAVVAEAILNGKEVSAARDKLRAEYASLSGWR
                     PIEEVQGKYVRGLFTGGTLCYEAQYIAQSYINPIYSNAPLRKEFKLENSLKPIGHSFI
                     DYGEDEFTQGRLHPMIDPSFRAEQLKEQSEDNSVAVVLFDVVLGYGSAENPSQEVAEA
                     IGAVKREIKPVYVAYVCGTAGDPQDLAKQISLLEDAGVIVCESNARAALLASSLISRR
                     ER"
     gene            40374..40553
                     /locus_tag="PKL93_05360"
     CDS             40374..40553
                     /locus_tag="PKL93_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70724.1"
                     /translation="MSFKDLLNSGPKVINLGMERFYLDLLEQEVPAVKVNWRPPLAKS
                     SLMDRLRKLRGEEVE"
     gene            40537..41802
                     /locus_tag="PKL93_05365"
     CDS             40537..41802
                     /locus_tag="PKL93_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HNQ70725.1"
                     /translation="MKRLNDIDKANSIAFERLLKAEPQIVGLGMAKEFIPNFGEKMIL
                     HAGPPITWERMSGPMIGAITGAILYEGWAETPEKARALASKGEITFEPCHHHNSVGPM
                     AGIISPNLPVWVVEEKNSGRRGYATLNEGLGKVLRMGAFSEEVIERLNWMREVLYPVL
                     RNGVRKYVEDNGGLNLKSLISQSLHMGDELHNRNRAATSLLIRTLVPSLIDAERGNHN
                     LSRVVKFMAGNDHFFLNPGMAAAKASLDSVGYVEGSSMITIMARNGTDFGIQIAGIEN
                     EWFTCQAALPDVLLFPGFTKDDVNRDIGDSAIMETYGVGGFALAAAPAIVQFVGGTPQ
                     DAVNYTKEMYEICVGENKHFTIPVLNFRGTPTGIDLIKVVETGITPILDTGAAHKEAG
                     KGQVGAGIVRMPSEAFVKAAEAFVEKYSK"
     gene            41815..42606
                     /locus_tag="PKL93_05370"
     CDS             41815..42606
                     /locus_tag="PKL93_05370"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNQ70726.1"
                     /translation="MVPKNWDKIRIYELSQPISHLTPPWPTYEPLQIKFFKRLAPNGA
                     NGQLLTHSNHVGTHLDGSLHFCTHGRDIASIPLNELVAPGVIVDLSDIAEDYGIYTSK
                     DIEERVEVREGDILIINTGYHKYAYDQPEADEVRYMIKHPGPTREFAEWCKMKKIKWI
                     GVDCGSADHPMNTKIREWMPVQAKECDRYMHEKYGKSLEEIFPDEDYQLMHVLLFPYD
                     IIHAENVGGEIDKILNKRMVIGCFPWRFVGGESCISRIVAFDEEE"
     gene            42634..43509
                     /locus_tag="PKL93_05375"
     CDS             42634..43509
                     /locus_tag="PKL93_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNQ70727.1"
                     /translation="MRDFVLERPRSISEVLDTLQRHGNKAMVKSGGTDVIVWLHKSIK
                     EPDYIVDLTAVEQLKGIVVNEDNVEVKALVTLNEVIGDDSLNRYFPALVQACKAHSDP
                     LIRNRATVVGNVCAAVPSGDMIAPLVCYDAKVEITGPNGSRKVPVSEFITGPKRTSLG
                     TDEIVTGIRIALPAVETSGCYLKAIRREALDIAQAAVCCTLFKDDNREFRIAFCAVSA
                     RPLRATEAEKLLNSCSNIDDEVIDQAAELAADAVDPITDVRASREYRVEMIAELTRRA
                     ISRCLERPAGEGVHK"
     gene            43506..44009
                     /locus_tag="PKL93_05380"
     CDS             43506..44009
                     /locus_tag="PKL93_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNQ70728.1"
                     /translation="MKDKRVTITLNGRKTTLVVEDRTTLLNALREQGVTSLKRGCEEG
                     ECGACTLIMNGLPQKSCLILAREAEGADILTVEGLVKKGRLHPIQQAFIDEGAIQCGY
                     CTPGMVMTTYALLKKNPSPSDEEIREALSGNICRCTGYLAIIRAVKKSAAILSGNNGG
                     GGTREDY"
     gene            43996..46410
                     /locus_tag="PKL93_05385"
     CDS             43996..46410
                     /locus_tag="PKL93_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNQ70729.1"
                     /translation="MKTIKTKTGIGKWLPRKYDLEKAEGTLQFADDLRFGSELLHAAV
                     VRSSVAHGEILNVDDSEALKIPGVLKVLLGEDFPHHFGLYLKDRTPMAIGRARYVGEP
                     VALVVAETEEAAAEAARKVKVTYSELEAIFDPVKAATNNSVLVHPDLHKYEHVDFITP
                     QPNTNIANWFRIRKGDTEAAFKNSAHVFEETITCPQIAHGFIETFCTICKQDPGSGEI
                     TIWTSAQSPFTVRDIIAKGINYPLHKIRVIAPPIGGGFGGKAGMTVEALCLAAAMDED
                     IKGRPVKLFIPREEVMLSSWVRQGFVARIEMAVSDDGLMQGIRNTFWFDTGISAEYGA
                     NPVRSAGYTSMGAYNIPNVWADCYAVYTNKPFGGAYRGFGLPELMSALEVVVDIVAHK
                     LKIDPVEFRKKNLIKKGQTTCTGMPMHDHALEKIVDKVVEKMDLHKEEPATRKGWKRG
                     KGIALAIKAPAIPADASSSAIVKLNGDGTVEVLAATMDMGQGTYSAFAQIVSEELGIP
                     YDWIKVYYPDTQSHPYDWQTVASRSCWSMGMAVKRAASELREKLLALFAEYWQTPVET
                     ISIEYGVVKCHKLGKSERIDERVQNGFKMPDGILKGGPIIGTGSFTPPDLIYPDPDTG
                     QSSKVVAHFTLGAVGIDIEVDPGTGRILVNKVCAGYDVGKAITPINIKGQIEGGTVQG
                     LSACLMEGLSYDERGRLLNPDFTDYKIATIMDAPTEIESFWEEVPEEISPYGNRGVGE
                     HSMIAPAPALNNALFKATGMRIHSYPLSRERVHKALKAKEKGETDFWEWPYELDLAYM
                     RAKKEW"
     gene            46755..49898
                     /gene="ileS"
                     /locus_tag="PKL93_05390"
     CDS             46755..49898
                     /gene="ileS"
                     /locus_tag="PKL93_05390"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNQ70730.1"
                     /translation="MPRFEDVDLKRPAAEREEEILSYWRKDDLATRSVTEREGSPEFV
                     FFEGPPTANGMPGIHHVISRTLKDAVCRFKTMQGYQVKRKAGWDTHGLPVEIEVEKQL
                     GFSSKQEIEDFGIEKFNQKCKESVWKYESQWKKLTERIAYWIDMEHPYITMENDYIES
                     VWWILKQYFDKGYIYEGHKIMPYCPRCGTPLASHEVAQGYKDETIDSIYVKFRLKGKD
                     NEYFIVWTTTPWTLPSNVALAVNPEFNYVKVEVKVDGDEVEYYYLVKERLSALLGEES
                     NYRIVQELKGMDLVNLEYEQLIPFAKVDKKAFYVVYGDFVSLEDGSGIVHMAPAFGED
                     DNILGRKFGLPLLQLVDKEGKITEEVTPWAGMFVRDADPLITRYLKDRNLLFKKERLT
                     HSYPFCWRCDTPLLYYARKSWYIKTTEYRDKMIEVNNSVNWYPKFVGEGRFGNWLENM
                     VDWALSRDRYWGTPLNVWKCDECGRLTAVGSRKELVERAIEDISEDIELHRPYVDDVH
                     LRCECGGRMTRTPEVIDCWFDSGAMPVAQHHYPFENKDNFMGDLFPADFICEGIDQTR
                     GWFYSLMSISTFLFGQSPYKNVLVNNLVLDKNGQKMSKSKGNTVDPWKIIEKYGADTL
                     RWYLLAVSPPWVPTKFDEEGVKDTFSKFFGTLHSVFSFFTMYANVDNVDPGEFDLPVS
                     EREEVDRWIISRLNTLVREVTDLLNHYDMTKSVRAIQDFVVEEVSNWYVRRTRDRYWT
                     SELDNSKKAAYLTLYEVLLTVAKLMAPIAPFTAEEIFRNLTHKEKESVHLEFYPVADE
                     SLIEPDLEKRMAAVMDIVTLGRACRNKVQIKVRQPLPKILVDGRIKKIVESMRELILE
                     EINVKNIEYIEELGDYVNYEVKPNFRVMGPKFGKDLKAIGNALRSMKPADVVARVKHE
                     GKISIEAEGKTFELTEEDLDIRIQELEGFTFEMSNENFVILDTELTPELLQEGLAREM
                     VSKIQTMRKEADFEITDRINVSFSGPREVVDAIDTYREYIKEETLSNAVVFEESLDSG
                     TEWNLNGYDTLIRVQRV"
     gene            complement(49956..50747)
                     /locus_tag="PKL93_05395"
     CDS             complement(49956..50747)
                     /locus_tag="PKL93_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNQ70731.1"
                     /translation="MDLFKRQLAPLTSEAWEEINERAKQVLKSYLSARKVVSVVGPKG
                     WEYSFVPEGRVKILEKEGEVGVGLRKAKPLLEVRVPFKLVRWEMDDIERGAKDSDLGP
                     LEEAAKQIALFEENVIYNGYEKAAVKGIVESVENKPIPLGSKPNEIIESLSKAAQVLK
                     DNFVDGPFALIVNPETMAILNSHVSGYPLVKRIESFLGTDIVVSRVVKDALLLPKDHE
                     DLELVIGGDFEIGYQSHTDKEVELFISESFTFRVLDPAIIVKLTV"
     gene            complement(50763..51095)
                     /locus_tag="PKL93_05400"
     CDS             complement(50763..51095)
                     /locus_tag="PKL93_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNQ70732.1"
                     /translation="MSNYHEPFEQLSEKAKDISRALNSLKEEIEAVDWYNQRVDVTED
                     AELKAVMAHNRDEEIEHACMTLEWLRRNMDGWDDELKTYLFTKAPITEVEEAGAGSEN
                     SGLNIGKM"
BASE COUNT        13812 a        11782 c        12070 g        13507 t
ORIGIN      
        1 gcgatccttc cagatttcgg agttcttgaa ctgttatcca caaatggaag tatcagcatc
       61 tttctagatg actcgatccg tgcagagata agcgcatcaa caactaatgg aaccatcaat
      121 ttgaaggagc ttaccgtagg tgtaatcagt tcgagttcaa ctagcttgag gggttctatg
      181 aacggtggta atgggctctc aataaatatg aaaaccacta actcaaacat tactatcgac
      241 aaacacaatt ccgaagattc tatttagtac agcaaaggtg gatttgaatc atggtccatt
      301 taccgtacta atgcgttgtg tggattttca atagtcttag agtggtaagc tagaccatct
      361 tcttctttcg aattcaggat ttagatctca ataaggttct gaatcacagg ttgtgacgtt
      421 acaaaggatc tatttgcttg tcgcctccct gacacggtct gaatcgagta caaagagtgg
      481 cattatcaac agggttaaga taatgaatag ttggatgaag aatcatctgt atttgatccg
      541 taaaggaagc gggatctgat agacaacaat gattacccaa atcttattct gcagagcaaa
      601 gatcccgaaa agagcataat ttcatctcac tgatttccga tctggtcaga accgagtgcc
      661 tgaactgaag aggcatgctc cagtaagaag ggcttgtgat catgttcgat atgctgtatc
      721 gagcagtata tggcgatgaa taacaaacaa aaccggaggc aattacctcc ggttttgtta
      781 cgaaagtgat tctagttaga gactgtcaac gggacgttct ctgcaagctc tatgaggtct
      841 acttcaaaga gtcggtctcc gatatatttt attcccttga ttccgagctc gtctatgaga
      901 gaggccgctt cctgattgga tgtgaaggta attctcccgg ccgattcgaa agaagcaata
      961 gaccagttat acgatggtgt tggattgata gtaccctttt cgataatgta gtcgatgatc
     1021 acgcttcgat tctcgactgt cgatgaaagg acaatatccg cgtcgacgag atgataaccg
     1081 ccgccacctg cgcggtaatt gtttgtcaca acaaggaact ccatatcatc ttcgatttct
     1141 tcgccttcat aagtcaaatt gacaatcctc tgacctacgg ggtttcgaac atctatctga
     1201 tagtctattc cttcgattac gtcgaagttg tatgaagaga accttgcgag caaattctgt
     1261 tcttccgtgc tgttaggatc gatctgattg aaattctccg cgctcttttc aagccagcct
     1321 ttcaattcga gaccattcac cttcacaaca tggagtgtgt tcgagtatat atagatatct
     1381 gcagcgttca taattcttac gtcccctgcg tctacactgg tatttgttct aaacggagct
     1441 gcagctgaaa ggagaggcat agactcatat tcagtgccct tgaaatactc tttaacgtac
     1501 caaagctggg cttcgttgac gagctgagtg acttcgttat cgattactct gctgaagtat
     1561 cccttgatcg gtacggttgt cgctccaacc ggggccatta cataatcaat ggttgcttcg
     1621 tgctgttctt tgactgcttc cactatctcc ggagcagctt cgacttcttc atcgacatgc
     1681 cagagctgcg ggcatgaaga gaatatctcc cactctccat cacggttcag aagatggaaa
     1741 tgtatcagac ctaaggagct tccccagctt cctgccattg tcaccgggac tccatctatt
     1801 ttgtcggcaa ttttcccgtg gctgtgaccc atgatgatgg cgtctattcc ttcaacgttt
     1861 tcgagtattg cttcagcgtc tgagcttccc tggtgaaggg agactatcac cagatcgacg
     1921 ccctcttcgc gtagtttggg cacatacttg gcagccgcgt ctactatagg ttcagcatag
     1981 acttttcctt cgaggaggac tctgtcccac atcattattt ccggaggtaa aaaggctata
     2041 acgccaatct tgattggaat tccgtcgact tctctttcca atatcacata ggggtcatat
     2101 ctgagctctt gcgtctcttc gttgtaaaga tttgccgaga tcatcgggaa gtcagcggaa
     2161 gcaatggcca ggtccagata ttcgagaccg aaattgaatt cgtgatttcc aattccaact
     2221 acgtcataat ccatgatatt catggctttg attatcgagg gagtatctcc catcttgatt
     2281 ggatctattc tcgcttcacg ctctgcgaga acgcttcctt gaatggcgtc tcccgtgtcg
     2341 atcaagactg tgtttctgta catctccctt gccatcttga tgagagtgaa ggttttggca
     2401 gcgccgtaat cactcacttc ttctacagtc aggtaatcgt aaggcataac atagccgtga
     2461 aggtcagttg ttcccataac tacgagttca tgaatcgagg tctttccgaa aaccattgcg
     2521 gggatcagcg cagcgagaat caggacgatg acaactgcag gaattccttt cttccagttc
     2581 aaaagctcca cctccatttg agttgaccag cccaattttc gccggataga attattgacc
     2641 catgaccgta agaatccaat gctgaagata attgaggatt ccaaagaatg tttcgaaaca
     2701 gtactatctt actacaatca tgtggactca ttaagaaaga aaaagcacat ctaagtaaat
     2761 gaaagtaggc agtctaatta acgaagggag aagggagaac cgggaccagg gttgtggggt
     2821 ctagggtctg gcaagagcag aagtgatgcc cttttagaac atttgaccca atgccggcga
     2881 agatcgggcc gggacgcaat gcactgagga gcatcagtgg actcaaggct gccttcggca
     2941 ggaggcctta tcaggtccac cgtcaacggt tctccgtcct ccgagacaaa gaacagaagt
     3001 gattctacta taaaacataa gacgaaatgc gccatgaaac gtcggcggtc ttagggctgc
     3061 aaaaaaacat gccagtctgc tgcgcaggcc agtcaggctc cgcctggcca gtctctgcct
     3121 ccggcagagg ccagtcacct tcggtggcca gttccgcttc gcgggctagg agccgctgaa
     3181 cgcttaagaa cgaaaccccg ctggtcgctg taaaaaccga gaaagagcac gtcggagacg
     3241 ttcgcttcgc tcacgagaag atgagagcga aaatagggac tgacaattct cctcatgtct
     3301 gtcccatttt tcgcgtaaaa ggcagaaatt ccaaccagga cctttttcgg gcgaacggcc
     3361 gttcgcccct acaagagaac tgcacagtca tcttaaattg tcatcgcgta gcgcctgccc
     3421 tgaaaagatt ctgttcaggg aaactgcacg ggatctcgct ctttaagaac ccggttctga
     3481 caacccgctt ttataaaccc gatctttcta acccgcactt cgtaacccgc ttctaaacaa
     3541 ggctcttctg cccgactaag tcggaactgg ccgcacgaag tgcgactggc gtgcgttagc
     3601 agactggctc ccaaacgctc ctattgtctg tcccgttttc cgcgaaagac agggatcctg
     3661 aacaggagca ttgtcaggat gacaagaagg gagagtctcc tgcccttaaa tcatgttgta
     3721 tcatccggtt atttccaagc actcttatcc cgtagagtct gcgccaatat ggattatgtt
     3781 cgttatccac ttccgtcatc ccgtgatgct tttgcacgcg atctcgcatc taagaaccgc
     3841 tgaacgacag aaagcaagga tccgctgatc ggtgttaagc agagacatca tctctattct
     3901 cctctttggt catcccgata tgaaactgat cgggatctcg tatctagaag ctgctttaag
     3961 ctgtggagat cagtttcaag ttgcaagcta gagaaccaga agagagtttc cgatgagtgc
     4021 tgttttttca tgactggttg gagtccaaac gctcttgaat catcaccttg cccaagaacg
     4081 aagaacagat cttcgctctg gaacgaatag caggttttcg tcggcgaact gaggacgttt
     4141 caacggtcaa ccagtttctt ttgctcttac gaacccgcaa ccccggtatt cagcgttaca
     4201 tcccgggagc tatccccaga gatcggtgtg atagttttct ttcttaagtc tgaatttgat
     4261 ctggaagtct accagttcct gccacattaa agcgctggtc gtagagacga aactaaccat
     4321 ttcatttgca atcccaaaga ccggttctat acttttttct atactggagt tatttatgac
     4381 gctataatcc agcttgtagt ctccgatcat cgcgtggtcc tcgtatatgt ttgcagttac
     4441 cctctctcca ttgaatatta cctcatcctt tatggggaag gaaaggccca tgcttccagg
     4501 cattatcctg tcgtggattt gaattacttc gtttacagtt ggcttggtcc agctttttcc
     4561 gcacgaagaa catgagaagc cagtcttgaa gcctttgata gaggcaacgg cgccacagct
     4621 tggacagcgc cagatcagct tttctatacg tttgtagttt cggtaaggaa gcttgaaggt
     4681 tggatcctcc ttggcgagga agcccatgac tttttcttct agctggggat catctgcttc
     4741 gaccggatca ccgaattcta cggtcgaagt ccccaatgca aaaatctttg cccatctagg
     4801 ccaaagtctc tgtactccat aaaacttcac cgggattaca gggattttca acatcctgaa
     4861 aagcttccat atctctcttt ttgtatgtag tggtcttccg tcccatgatc tcgctgcttc
     4921 aggtgcaatt cccagtggaa ctccctgttt tattgtcgaa agcatctgtc ttatggcgta
     4981 tgtatcgaca gcatatcttc tgacaggaat tacggcgagc cagttaagca acccgagtct
     5041 atcaacgtag attgcggtgc ctacgaacct caggtaacgc cacgtcgagt aaaagataag
     5101 aggaccgtcg aaatgagttt ggtgagcaag agcgagaacg aagggcctct tttctctgag
     5161 cactcttgca ttctttacct tagagggtag aacaatcaga attaccagct tcacaacgaa
     5221 tgatgccacg atgaattgcc agggaatgga tgtcgattta tcaaagggta tctttcttcc
     5281 gggatttggg atcatgaaag gtatcttctc tttatagtcc tcgtagcctt caattctttt
     5341 cagagcgagt ttttcttgga gcaaagaata cacaactatc actgcgccta tgactgcaac
     5401 gtaaagaagc gtccaatagt ttccactcaa caatccaaga ccgaaaagga aaagtatgta
     5461 acccaggttg aatggatggc gaacaaatct gtatgggccg ctttgaacgg ttttttcggg
     5521 cccataaata acggtcggca gtcttccgga ccagtaatat accacaagag cagaatagat
     5581 cgctagaaga agagacaaaa tgatgataat cgaagcgact atcgtcactc ctaatcccgc
     5641 tgaaaacggt gctccggaaa agaggagaaa gggaatgatt atccaggtta ctgccagctc
     5701 cgcaaccgat atagcaattc tcatcttaat ccctccactt tgaatactgc tcctccatcc
     5761 tcacaattat ctgcatcgat tgagagtttc aacaccttgc agatcttgct ggcaatggaa
     5821 agacccaatc catgaccttc accacctctt gaagagtcgc ccctgtagaa tctgttgaat
     5881 attcgttctt tttcatcctc gttgattccc atgccatgat ccctaacact taatgtacca
     5941 tctgaccaat tgatctctat cgggccattc tttccaaact tcaaggcatt ttcaacaaga
     6001 atttttacaa ttatcttaag cagaactcta tcggttcgga cttcgatatc tggaatctcg
     6061 atcttcatct ctgccgactg atcggctgga acgctattgg tgatctcgag taccagttct
     6121 ctgaggttga aacttgtttc gtttagttcg atctcgggtc tcgagagaaa aagcagagat
     6181 tcagtaattg tcatcatctt ggtggctgtg tcggagatac tttcagcggc ttccttgaca
     6241 acctctgggt cgtcagaacc ccagcgaaga atcagtctcg agaagccaag gattgaagtc
     6301 agtggagagc gaatctcatg cgcaatgtct gatgaaaact gttcctgcct ggtgtatcca
     6361 tcttgaattc tgtctagagc tatattcaga gcttttttga gttcggcaag ttcggaagtc
     6421 ttcggatcgc tttccagtct tcttgaaaga tcattcagcg ttatctcctt taacagacgt
     6481 gaagtttctt tgataggttt agagaccctg cttccaagga agaagccgcc tatgattgaa
     6541 aggacaacac caaccaagac tgaaagaagg agagtggtgg cgagactctc taggaagaaa
     6601 tcgagatcgt ccattcttgc accgagaatc actttttcct ctccattcaa gaatccatag
     6661 agaagatagg agctgccatc tatcctcgtc tttacatatc cctcaacatc cggaacgctg
     6721 ctcagtcctg ttggattctg aaccacgttt ccatcaacgc tcacgtaaaa ctctgtagtt
     6781 cctctgccta tggccattct tcccacgcca tacctaaacc ctttgcccat gggcgaatcc
     6841 atgaagatct ttctcatctc tgcggagtaa ttgcgaatga agacactttc ggagactcca
     6901 taaacgaata tggccgaggc aaagagaatg gatacgatca gaaccaccga gtagatggct
     6961 acctgagtcg ctgtagagag cttcctccta tctgacaacg tagccagagc ccctcaccgt
     7021 ttttattctt tcgcccgtct ttcccagttt cttacgcaag tagttaatat atacttccac
     7081 aacattgggg ttgccccagt cctcgcttcc ccaaaccttt tcgagtaatc tttcctttga
     7141 catcacaatt ccagcgttcg atagcagaac tgccagcaga tcaaactcgg tcttgctcaa
     7201 ttgcagaggt tctccattga gaaaggcttc acgagattgc atatcgaggg tgatttcgaa
     7261 gtagctgatc ctttccactt tccctttgcg tctaagtact gcttcgattc tggccagaag
     7321 ttcctcaatt acaaatggtt tcggcaagta atcatctgca ccgctcttca aacctcgtat
     7381 tctgtcctta acttcacctt tagcggtaag cattataacg ggaagatcgg tggaaatata
     7441 ctctctgatt tcttccagca ccgagaaacc atccaaaccg ggaagcatca cgtcaaggat
     7501 aagaagatct ggaagctctt tttctagatg ctttagcgct tcctttccat cggtcaaaac
     7561 ttctacttcg tatccttcat ggcctagctc caagcgaagc aattttccaa tatgcatatc
     7621 gtcttctacg accattatcc ttcctttcaa cccttcttca cactcctatc tcgcattacg
     7681 aaggacgaga tccctccaag taccattcca gcagtcatga gaagcaaacc caccacttcc
     7741 attttacccg gaatctctct cagtatgatc attgcaaaga acagtgcgaa ggccggttca
     7801 cctacgtaga tcagagaggc tcctacggaa ccgagaaacc tctgatattt caactgcact
     7861 gcaaccgcga agatagttgc gaaaactgca gaaaacagcg caacaaagag tattgaaccg
     7921 tttataaaca cacttccttt gacgaaagga atcagcgcca tattgacaag aagcactacg
     7981 acaaattgag gaacgagaag ctccatttcg tcgacatctt ccttctgggt gaaataagag
     8041 atcaaaacga catgcagggc gaaagaaact gcgcaaagag cggttagcag gtcaccaaag
     8101 ttgaatcctg tctcgggtgt tgtaagaaaa tacaagccga ttaatgatac ccccagagca
     8161 agaatctgat agcggctggg gattcgcctg tctacaacgg gaccaatgat tgcgaccaaa
     8221 ccaacgtaaa gagacgtaat aaaagcgctc ttggatgcag ttgtatagac aagtccccac
     8281 gtctgaaaaa catatcctcc cgaaaggaaa agtcccaaaa tcaaaccttg agtaaggctc
     8341 ttatttgtga tcttgaggcc gagcttcttt ctcaggggaa tcagaaccaa gagagcgatc
     8401 gaaaaacgaa gcgcattata gaataccggg gatagaccct ccagtatgtt cttctgcacc
     8461 ggaaaggtca atccccagaa tattactacg agtaataggt ttacgtatgc cttcgacttt
     8521 agcttcatct aacctggaag tgtcttcttc ggggggctct taatcagatg cgccgagtat
     8581 cactgcaatg acaactcccg ctatcagcac tccaattatg gagaagagaa taatgttgtc
     8641 ggttttcttc tcttcttcgg ttttctcggt tagagtgaaa ctgactgagg tcaccatatc
     8701 tttgtttatg tacacgttct gcttcgagtc atgataaccc catttggaaa cggttactgt
     8761 atgactccca actttctcga agtccttgta tggagtgtcg ccggcgtatt gtccatctac
     8821 gtaaacctgt ccgtcgggaa tcgatgtgac ttccagataa ccgtaaacgt aatttggcag
     8881 tagtgagaaa taaaaatcgc ttgacccggg ctggacattt atcgtcgcag gaccataaac
     8941 aaggttcctc tccgtgtaca catacacagt atgaggaccg gcttcaagtg ttgtcgtaac
     9001 cggggatttg cccatgaata ttccatcgac gtagacgttg gcatcctttc tgtctgcgtt
     9061 tatcgttgtt cttactgtga aaggaacata gctgttgtag aagtaggtgt agcccaatcc
     9121 ccagcttgtg ttgaaaaccg gatcgataac taccgaaagg ttgttgatgc tctgactcaa
     9181 gtagggaaac ggatatctgt cgaagagaga gaagacgttg cccaggaagt tcaacggaga
     9241 tgtggaagca acgactacga tagtctctct tcctctctgt gactggacct gtagattgta
     9301 cctcgttgta gctttgtcgg tcggcaaggt gtaggttctt ccaccctgca gatagttgtc
     9361 ttggtcatat acgtttggga agagaacctg aatctgacca gacggatcga tgtcaagaac
     9421 tgccacgtaa gcacttctat ctactgaaaa agatagtccg agcgattcac cgccataata
     9481 agttgatccc atacctttgt tcagacttac gttaacattg atactcgccc ccggtctgct
     9541 caagaacatc agcccctgtg gatcgtacga aaccgaggtt gcaaaaagag aaacacttaa
     9601 gaatacaatt gctaagagca aagccttttt cacgctaatc cctcctcatc ccttaggttt
     9661 tttatggcat tggcgataat tcctgctgag tttgatagtc cctttaattc cgctgacgac
     9721 aggcctacct tcagaatcct ttcaactccc tctctgccaa gtattgtggg aacgctgaaa
     9781 ggaacatcct tcagaccttc aaactccggg tgaacgctgg aaacagttag aactgaatgc
     9841 tggttgttga aaattgttga aagcactttt gatacagcca ggcctattcc gtaaaatgtt
     9901 gaacccttct tttctataat tttgtaagca gcatttttag tttcttcgaa aagcttctgg
     9961 aagaggtcag cctcgcagtc tctcctgcaa tttctgcaga agtcggttag aggaattccc
    10021 gcaatgtctg cgtgagacca gttgacgatc tcgctgtcac catgttcacc aagaacataa
    10081 gcatgtacgt ttcttgggtc aagctggcag tggtctgcaa tgctctgtct gagccttgcc
    10141 gtatctagag tcgttccaga gcctactatc ctgtttcttg gtaaaccgga cgctttccat
    10201 gccacccatg taagaacgtc gacgggattc gtcacgatta tgaaaactgg ctctttgctg
    10261 taggaaagag atttagccac cgcttccttc actatccttg cgttgtcctt tagaagctgt
    10321 aggcgactct ctcctccttt ttgaggtcta ccggccgtta ttacgactac atccgagtct
    10381 gaagagacat cgtaatctcc gccatgaatt ctcattggtc taacataggc attgccatga
    10441 ctcaagtcca ttgcctgacc tttggcgagt tccctgttta tgtcaacaag agctatctct
    10501 cttgcaacac cttttatcat cgatgcataa gctattgacg agcccaccat accggctccg
    10561 actatcgtta ctttcatcat actcctcctg acaagaccgt cttgaaccag tgacggaatc
    10621 ttgcttcaag gtctgccctt tctacttttt caacgatttc gcctttcacg aaaatcactc
    10681 cgcctgaagc cgttccagcg atcccaatgt ctgcttcagt gccctctccg attccattga
    10741 ccacacagcc catcactgca accgagaggt ctgcatctgt gttgccgacc cagctcttga
    10801 cttttctggc gagagcttcg acatctattt ccgttctcgc gcaggttggg caggaaatca
    10861 catcgacccc ccttctcagt ttcagcgacc tgagaatgct caacccgacc tcgatttcct
    10921 gaacagggtc gcccgacatt gaaaccctaa ttgtatctcc aattttgttg agcaaaagcg
    10981 tgccaatacc gattgacgac ttgaccgtcg agtccaaacc aaagcccgct tcggtaacac
    11041 ctagatgaag tgggtggtca gtcttgttgt agaggtattt gtttgcttca accgtctcaa
    11101 taactgaaga ggatttcagc gatatcacaa tgtcttcgaa ccccgcgctc tcaagtattt
    11161 taacttgagc cagggcgctt tctgcaaggg ctataggttt ttcgtacgag ctgaatctct
    11221 tgtcaagaga accggagtta gctccgactc gtattggaac gccatattcc tttgcgattt
    11281 tcacagtttc tttgactttc caatcttgac cgatgtttcc tgggttaatt ctcacctttg
    11341 cagcgccgtt tttgatcgat tctacggcaa gtttatagtc gaagtgaata tctgctatga
    11401 caggtatcct gcttaaagaa gtgatctcct tcagtacgga agccgactca aagtctggaa
    11461 ctgataccct taccagatca caacctgcat cttcgagcct tttgatctgc tcaacagttt
    11521 tgtttacgtc agaagtcttg gtgtttgtca ttgattggac cgtgattgga tttcctccac
    11581 ctatcagaac atctccaacg cgaacgaccc cgctcatagc cacctaccta tatcaatgaa
    11641 agtaatgtaa atcatgaacc ccatcaagac catgaatccg atcacattga tcagaccctc
    11701 tatctttgga tcgagccttc ttcttgtaat catttcaatg agggcaagca acattcttcc
    11761 accgtctagt gccggaaagg gaatcagatt tattactccc aggttgagag atatgaaccc
    11821 agccaggtaa aggactattc ttagtccaag actgaccgcc tgatcaacta tggttaccag
    11881 tccaatgggg ccggtgaatt cgttgatgtt ggctcccccg gtgaagaggc tccctactac
    11941 cttaaccaga gctatcaata gattgtttgc ccactgaacg ccgagaccta ccgcctccaa
    12001 tgcagtatcg gggtaccaat aactgcttcc ttgagcaaag gctcccgcct cttcgaccag
    12061 ggctatcatc tcgtttttac tcactgtcaa atttattaat tgaccttccc gttcaacaga
    12121 aacaacgaat tcgtcgggaa agccgaattc ggcaaaggag atttctttcc cggtgaactg
    12181 aatcaagagc tcatcgggct tcagagccaa caactgactc atcgtggtta gatcataact
    12241 tgtttcagtc ttcataccgt tgactgaaag caggatgtct ccttctctaa actgtccgtg
    12301 gtcaatggca agttgaggtt tgaagtttgc ataatagatg cccattgcgt agcgttcctc
    12361 ggcaggtgaa taagtcagga gcgttcccga taatgtagag ccatcttcga agccgagttt
    12421 tatagaactt tcaggcgaaa gaacacttcc atatccaaca taatcgccgt tgaaggttcc
    12481 gccgtttatt gaagagagaa tatcgcctgg ttctcctgaa acacctccga taacaaagaa
    12541 ggcttcctgg ggaagaagtt caggtattat agcgatctcg atttccttac cttccctgtt
    12601 cacaacaaga tctattctct ctccttttgc tactgcttca ctcaaacgtg tattgtcgat
    12661 gagagtcttt ccattaactg aaacgattcg gtctcccggc atcattccgg aaactgcggc
    12721 aggagaacca ggttcgactt tatcgatctt gacctcttga aagccccata ttactgcgac
    12781 aagagagaga attaaaaagc ccagaagtaa agagaaagct ggaccggcga agtaaataat
    12841 gaatctctgc caggcgggtt tgttctgaat ctgctcactt tccggaatcg aggagtcgat
    12901 agtatccaag tcttcacctg caggtcttac gtaaccgccg attggaaatg cattgatcct
    12961 gaaggtcgta ttctttcctt ttttcgacca gagcttaggg cccattccta ttgcgaattc
    13021 aagaactctt acaccaaaaa gcctcgagaa aatgtagtga ccaagctcat ggacaactac
    13081 tattcccgtc agaattagaa gaaagtatat tattgctaat aacatctatc cacctcttcc
    13141 tgagctaact ttcttgcaac gctgtcagtc tcgattattt gactgtagtc gaccggattt
    13201 tgatcagcgg ttctttctac tgtcttttca attatattgt aaatacctcc aaaaggcatc
    13261 cggccattca taaaagcttc gacggcaact tcgtctgcag cattataggc aatctgttta
    13321 cttactactc cacagatctc tctcgcaagc gttagagcag ggaacctctc ttttggtaac
    13381 tcttcaagaa tcatggggac atcgacaata ctttgctcgg gaatggtttg aattctttca
    13441 ggatatgtaa gcgagtaggc gatcgggatc ctcatgtctg gtatgccagt gtgtaacttc
    13501 aagacaccat cgctataaag gataccagca tgaataaaac tgtttctgca tatgtatgtg
    13561 tcaatttcat tagtttcaaa ttcaaagagg tatttggctt caatcacttc aagtcccttg
    13621 ttgaccatgg aggcactgtc gatcgttatc ctgtttccca ttgaccagac gggatgcctc
    13681 aaaacatctc ttatcgttgc atcttgtatc ctttctaagg gccaatctct taatgccccg
    13741 ccagaagcag ttattataat tcttttcggt cggcgtgaac catcgaggag ctgaaaaagg
    13801 ccgctgtgct ccgagtcgac aggaatcagt tcgacgccct ttctggcaca ctctttcagg
    13861 agaagatctc ctccacaaac aatagattct ttattggcga ggcaaagcct gtgacagact
    13921 tcgatggcct ttaggctgaa tcttagacct gcggcgccgc tcacagccag caaaacataa
    13981 tcgggtttcg attcctcaag cattctttcg attccctctt ccccgtggaa gaataatgtt
    14041 gcgggaaagg aatctgtaag gctatcgctt ttctttagtg aggcggcaaa gggcggatga
    14101 aattcagaaa gctgcttcga aaaaagagaa agattgtggc cacaagaaat ggccacaatc
    14161 tcaatattct caatccgatt tgctatctgg agtgtttgtg atcctatcga tccggttgag
    14221 ccaagaatgg ccagcctttt tttcatgatg caaacttctt ctttatctcg gccggttcac
    14281 ctgatgtctc ttcgatgatc ttctgaagtt cggcttcaga taaagccatg atcttggcaa
    14341 ccttttgcga gacattctgt ggctttccag gaacacattt cgctatgtct tcgtctttat
    14401 tggcaacctt aatggcaaag gcagcacatc ctggcgagcc gcacaaggcg cagttgatgt
    14461 tgggaaggac tgcgaagata acagctgccc ttggattttc tttgagtttt tccttgtaac
    14521 tatcgatttc ttcttgcgtt ggcctttgta tctttggtgg agcggctttg acggcttttc
    14581 ctgaagaagc tagtagcttc tcgagagttt tttctgcatc ctcttcattt tcgtcgaaca
    14641 aggcgatgag tttgtcgata tctgtgtcca gaatgagttt taacttctca gggacaccgg
    14701 agcgtttacc tggaatgcat ccttcgatgg gggctttacc ttcagcaaca gctttggcaa
    14761 aggctgcgca tcccgggaag ccgcaggcac cgcaattagc accaggcaat gaagcttcga
    14821 taagcgctat tcgtggatct ttcttgacgg cgaattttgc cgaagcgaac gctagaaaaa
    14881 tacctgcacc tacccctagt actgacataa agagaactga atagactatt gtcacagtaa
    14941 acacctcaca gttttaccag gccctggaat cccatgaaag ccatcgacag cagtccggcg
    15001 gtgatcaagg cgatagctgt ccctttaaag ggttcaggaa tttcggagag ctccatcctt
    15061 tctctaatgg ttgaaaagag aattagagct agggcgaacc ccagtccggc tccggctgaa
    15121 ttcactatcg tttccactag tgtgtagttt tgctggacat tcagaagagc aaggccaaga
    15181 atcgcacagt ttgttgtaat aagcggtagg aagatgccca gagcttcgta gagtccaggg
    15241 ctagcctttt taaggaatat ctcaacaaat tgcacgagtg tcgcgattac gagtatgaag
    15301 acaattgtac gcaagaaggg taaccctagg gcatctagcg caatattgag tatccaggtg
    15361 attatcgagg ccagtatcat aacgaatgta acggcgatgc tcattccgac tgctgtatcc
    15421 gtcttccttg agacacccag gaaaggacag attcccagga atctggaaag cacgaagttg
    15481 tttatcaaaa ttgccgatag aaatataaac accaatctgg tcgccatcag tcattcctcc
    15541 tcatttttct gcctttttta ccttgcttct cctaatcccc aaatagttga agaaggctgc
    15601 gagcatacca agggctatga aggcacccgg ggggaggatc atggcaaaca ttttcacatc
    15661 agaaagatgg tagccgaaaa tcgatccgtt gccaagaaac tctctcaccg atcccagaag
    15721 aaccagagag gccgtgaaac caaggcccat tcctagacca tcgaggaagg aactgaagac
    15781 atcattcttc gaagcaaagg cttcggccct tccaagaatt atgcagttaa cgactatgag
    15841 gggaataaac agtcccaatg tcttccatag atcatagaca aacccatgca tcaacatgtc
    15901 gatcacggta acaaaggaag cgattattac tatgtaagag ggaatcctta tcttatcggg
    15961 aatgaatttc ctcacaagag atattgtgat gttcgacaac gcaaggacag cagtagttgc
    16021 gagtcccatc cccaaaccat tttcggcgct tgttgtagtt gcgagagtgg gacacatacc
    16081 aagaacctga atgaaggtcg ggttttgttt caagataccg tttgccagaa tcttgaactt
    16141 cgattctgcc atcatctaac acctgcctta tcaaggaatt cggagatggc gttgagagag
    16201 gatgcgacgg ctcttggagt gatcgttgca ccggtcatca catcgctgac tgtcactatc
    16261 ccgtcttccc tctttcttgc atctatctgg tcacccacag gggttactcc agcatctttg
    16321 tcaaccctta tgcctttgtc aagtccctct ttacttacag gatagaacct cttctggaca
    16381 tcggcatttg caatatttgc tccaagtcct ggagtctcct gagagtattc tagaaccttg
    16441 attccattta acttgacccc atcaggctcc tttacaaagg ctgccattga tttcacgtcg
    16501 cctccgtaac caaccgcgat accaatcgca atgaagatct ccgtgccatc ttctttaacg
    16561 aatttgtaag cagggctttc aacctttccc tgattgtcct cggtcctgta gattatccct
    16621 tcagaggggg aaaatccttg gggaagccac tcgaatttag cgagctcggt agctgtttgc
    16681 gggatgcttt cttcgtcaat taacaactca cctgtatctg aatccgtaag aacttctcga
    16741 atcgccttca gctttgcgcc aagctctgcg ttggctatag gttcttcaac tatcgtatag
    16801 actaccgata gaaccagggc ggcgataatc gtaataacca tcagagtaat cccggtcttc
    16861 aagtaatcac gcattgctct tcaccttctt tggctttcca aatatcctgg gttttgtacc
    16921 catatcgatt agaggtacaa aagcattcat gaccaggatc gcaaaagaaa ctccttcggg
    16981 ataaccgcca aacaacctga ttatcattgt cactgcgcca caaccaactc caaagatcac
    17041 gtggcctttg atcgacatgg gacttgtcac catgtcggtt gccataaaaa gagcccccag
    17101 cattaatccg ccgccaaaga cgtgataaag aggtgaaccg aagccgggat taactccatt
    17161 gaaaattgcg gccataataa cgacagttcc aatgtatgca actggtatga aaggagagac
    17221 tctctttctg aacagcagcc atccaaaacc aaggagtagg agcagagcgc tgacctcacc
    17281 aagtgatcct ggaattgttc ctagaaacat ctcctgcaga gaaaagactc ttggtccgcc
    17341 ttgcttcaat atcgccattg gagttgctgt agtctgcata tcgaaaggac tcaaccacgt
    17401 agtcatctga acgggaaagg atatcaggag aaagaccctg ccaactaggg cgggattaaa
    17461 gaaattcttg cctagcccac caaagacggc cttcgcaatt acgatggcca caaagacacc
    17521 gataagagct tgccaccact ccacggcgac agagagattc agagcgagaa gcagtccggt
    17581 gacggaaccg ctgagatccg gtttgaagtt cttcttcctt ctaatgaacc gcatgacaaa
    17641 gaagtcaagt acctcggcgc cggctgtaca gacaactatc aggacaagtg ccttcaaacc
    17701 gaatgaaact acggcccata cagccgctgg agcgagggcg atgaggacat cgagcataat
    17761 ctttcttacg ctgtcttctg atctcaggtg aggtgctgcc atcattgaga gcttcacttc
    17821 ttcgcacctc ccttgagact cctgtagact ttcttattga gcttgaagtt ctttacgagt
    17881 tctatgtttg ctgggcaaac atatgagcag cttccgcatt ctatacagtc caggagtccg
    17941 ttctctacct gtttgtcata caatctattc gtaccataga gattcagcaa aaatggctgg
    18001 aggttcatcg ggcatacggt tgcacacttc ccgcatctta tgcaggggaa ctcttcaagt
    18061 ggatcgctct tcataagcaa agtgatcccc gaagtgcctt ttacagtcgg gacgtctatc
    18121 ttggggacag tgattcccat cataggacca ccaaagacaa ttctctcaac gatttcctgt
    18181 gcgccgccgg cgtattcgat caattcgctg gccataacac caatacgcga aataacgttt
    18241 aatggcttct ttattccttc gccggtcagt gtgactcctc tttctacaag cggatggcca
    18301 agttcaacag cttcgtaaac tgcaaatgct gttcctatgt tgtcaacaac gacacccacg
    18361 tcgagcggaa gtcctcctga aggcaccttc ctcttcgtaa gagcataaat aagctgcttc
    18421 tcggcacctt gcggatattt cgttttcaat ctcttcacct gaattggagt agacctcgcc
    18481 gccacctcca tttgttctat ggccttttgc ttattgtctt cgatgccaat tacagcgtta
    18541 tcaaccttaa gcgctctcat aatcgctaga atgcctcgaa ctattccctc ggagcgttct
    18601 agcatgtacc tgtaatctat ggtcagatag ggttcgcact ctgctccgtt cagaatgaga
    18661 aggtcgatct tcttttcttt tgggggagaa agcttgacgt tcgtagggaa catggcgcca
    18721 cccagaccaa caattcctgc gtcctttatc ctctggataa tttcctcggg ctcgaactgc
    18781 tcgtaaggct ttgcaggttc tagaagttcc cactcttctt cgcccgattt ttctataaca
    18841 attgcatcgt caggttttga aagagtcgga tggtagtact ttgcgatctc cttgacttca
    18901 cctgtcagag gagaatgaag atttgcggaa atgaaaccac tggcctccgc aatcaattga
    18961 cctgtcttta cgctgtctcc aacgttcaca agtggtttag caggaacacc ggcgtggttt
    19021 gcaagaaata catagaccct ttcgggaagc ggcagaacgc tcagacggga atctttagac
    19081 agttcctttt tctctggcgg gtgcacccct cctctgaagg taggcaacct catttagtta
    19141 tcacctctca accttaatca atagatgttt gcctttctgg gatcccgaat atcatgaaag
    19201 aaacttcgac tcaaaaaatg actgggatta acaaacaaaa ggagcggtcg ctccgctcct
    19261 attatagtac aatgtttgag tcttaactta atctaaaact actttctggt tattcttccg
    19321 ctatctgggt aagaggttat cgggctgatc tctccaacaa actcgactac aacgtcggcg
    19381 agaacgaatc cagtgtcgta acctgagagg ccagcaagaa tttcataacc gtcaccgcca
    19441 gaggcaaggt agttatttgt agccagtttg tacaacttgt tatcatcaat aggttctccg
    19501 tttacgagaa tatcctttgc ttctttgttc tcaattacat aagttactcc acttacctga
    19561 gccatcgctc cctgccccgc ttctctcgtt gccgtgtact ggagaatctc tctgagagcg
    19621 gtgccgggta cttccaaaac gtacagcgtg tttccaaacg gaagaactgt aagaatgtcg
    19681 cgatacgtta ttggaccagc ctctatggaa gctctgattc caccagaatt tgtcaaggct
    19741 agatcagcac cggttttcca taccattgcg tcacatatca agtaaccaag gttcgtgtcc
    19801 tgtgacctta ctactcctct atccccctcg agtcttatcg cggtctctcc gacaacgata
    19861 ttaagagctt ctccgccggc cttcttgaag taatcaagca cagttgccac ggcgaagtcc
    19921 ggttcgatct cttcggcgat aaccgggaga tttctgaagg aaacgaaaat gaccgtatca
    19981 tcaacgattt caagttcgag cttgccaaga ttcttcgccc attctcctgc cgatacgatt
    20041 attgtctcgt tgattatctc aggcctttcg tagaaagtgt ggctatgacc gtcgacaatg
    20101 acatcgatac cgtcaaccgc tttcgcaagc tcccaggaat tggggccggc aagcacggga
    20161 ttgcttccca tgtgagtgag cgcaattacg atatccgcct gagcctcgag ttcaggaatg
    20221 atctctttgg caacttcaat tacgtctttc cactcgtagt ctttcgcata gaggtactca
    20281 aggatttccg tttcttgagt cgtaaaccca acgatggcta cctttattcc tccgacttcc
    20341 gtgatcatat attcctggaa ggccggttca ccatccttgt agatatttgc gcttaagaac
    20401 gggaaatttg cgatttccat ctgtttcgca agtacggaag catcgttatc gaattcgtga
    20461 ttaccgatgg ccattgcgtc tagcttcatc aggttgaggg ctactatatc gggtgcagcg
    20521 tcctgcaaat ccgattcggg gacaccggtg ttgatatctc cggcatgcag gaatagagtg
    20581 ttgggattct cagccctgac ctgatttacc agggtggtta tagccgccag tccaccgtaa
    20641 tcaccttccg gccagatatg tccgtgagtg tcgttgatgt gcagaatagt aagcttctgg
    20701 cccatcacaa atactgccat gatcagcaac agggtaataa gcagaacttt cttcataact
    20761 catacctcct tatgaaatat cggtgatatt gccaacgatt tcaaaatcga aggatttctc
    20821 tccttcaatt atatggtaat ttattatctc tatttttgcg cagtttgaat aaactgtcct
    20881 tgaacttttt tccatttgaa ggccattgat ttccgtttct tcgggctctg caaggtatat
    20941 ggatttgata gtcagtgcga gtctgaagta ccatgaactt agccttcttg agaccattat
    21001 agtatccact ggtgccagta ccgtaaccat cgcagtacag actatcagaa ttgcgatgag
    21061 cagtgtaaaa gaagctactc ccttacgctt tgggggaaat ggttttaccg ccttcaacat
    21121 ctccctcaaa atcctcctct ctaacgtgat tgagaatggc actgttcttt cctatctttg
    21181 tgttttgtgg aataaccacg tcagatccga cgactacgat tcccgcgtta tagatgtgag
    21241 gctgcgtctc gtgtggaact tcatctccat agccgagaac tactccgtcc ttcacaaacg
    21301 ttccttcacc gactattcct cgttctataa caacgtttct gccgatcttt gaatttgtca
    21361 taaccacaga gtttttcacg agcgagccct cgccaatctg aactccttga aagattacag
    21421 agttttcgac ccgtccgtag atctccgcgc cttcgttaat caaagagttt cttacgtatg
    21481 agctcgtgcc aacgtaggca ggcagatact cctcggtttg agtatagaac ctccaggagg
    21541 gatcgtgaag attgaatgga gggatgggcc tggtcagttc gatatttgcc tcccacaatg
    21601 attcaattgt tccgacgtcc ctccagtaac cctcgaaaac gaaggcaaat agtttcccgg
    21661 cgttgtcttg gacgattttt ggcaagatat tctttccaaa gtcgttgtcg ctttcggggt
    21721 ccttctcgtc ttgtataagt ctttcacgca ggaacttcca gtcaaagaca tagatgccaa
    21781 gagaggcgag atccgacttt ggttccttag gcttctcctg gaaatccgtt atcttgttct
    21841 cgaaatccgt aatcatcatg ccgaatctgt gtgcctcaga aagcggcacg cgcatacatg
    21901 ctacggttcc agttgcgccc ttggagagat gataatctat catctcgttg taatccattg
    21961 aatagatatg atcgccagag agtattacaa gaagttccgg gtcaaaatca tcaacgaaat
    22021 caatgttttg gaagactgcg ttagcagttc ccttgtacca gtttgagtct ccttgagcta
    22081 taaatggagg gaggattaca actccaccgt cctttctgtc gagatcccag ggacgaccaa
    22141 tcccaatgtg acgggaaaga atgtggggcc tgtactgagt aagaacaccg actgccgtga
    22201 taccggagtt gacgcagttg ctcaaagaaa agtcaatgat tctgtacttc ccgccgaaag
    22261 gcactgccgg ttttgcaacc tcatcagtga gcaaaccgag tctcgtcccc tgtccgccgg
    22321 ctaatatcaa tgcaacaacc ttcttggcca tacaatcacc ccgcctaaat tacgccgtgt
    22381 ttttcgatca cagtgggatc ttcggcttca ccgacaaaac ttcttcccag cctcacggtg
    22441 cagtacttgt ccataatgac gttctctata taagctccct cttcaaccaa gcaaccctca
    22501 agaagaactg aattcttcac agttgcgccc gccatcactc tcgtatctct gaataagact
    22561 gaatcctcta ttcttccacc tattacgcat ccatcggcaa tcacagagtt attcatatat
    22621 gcagttccgg tgatctttgg aggtggaaga tccttcaatt tggtgaagac tctgccgttt
    22681 tcgtagaaga gctccctcct tatttcagga ttaagtatgt cgaggttcgt tctgaaatag
    22741 cactttttac tcagcttaat atcccgccaa tacccattgt acctgtattc tctaacattc
    22801 aatgaagaaa gattcggaga aattatccct ttcacaagct cattttcacc attggggacc
    22861 gcagaataga gcagttctct caataaaaac ttgttaatga aatatatgtc aagcgcaaca
    22921 cagtcgcctt cttgcgggtt ttccgcaatc ttttcctttt cgatccactt gacaactctt
    22981 ccgtttctac agaccactcg gtccattcct tccataccgc attcgccttc tttgttgcct
    23041 gtcaaaacgg ttatatcggc agccgtgtca agatggtatc tgaagatctt tgctagatca
    23101 gtcttgaaga tgtgatctcc tgagccgatc agcaccgagt cctcattgcc tcttctaaga
    23161 atgtttatgt tctggaagag agcgtctgca gttcccttgt atcccatgga aggattgtcc
    23221 ggagagtaat aaggctgcag tatgaaaagt cctccctgtt ttctatctag gtcccattcc
    23281 tttcccgaac ctatatggtc catcaggctt ctcggactgt actgcgtgat tatcccaacc
    23341 tttgatatcc cagcgttgac catattgctg agcgtgaaat caatggatct gtactttccg
    23401 taaactggca gtgcagcgct ggccctaacc tgagtaagag gagtcaagta ctcaccccgc
    23461 ccgcctgcta gaattatgcc gagaactttc atatgatcac cgtattccag atctggatac
    23521 ggcctccctc ccttctgtac gaatttgtca ttcagtattc cattagtggc aggtttgtca
    23581 taagagagac ccgtggcaaa gagaagtcgc ccactccatc aagataattt tgcaaaaaat
    23641 gaacggcgtt gtcaaaggcc tctttgtaca attcatggaa cgtcttcttg ctttcccaac
    23701 cggcaaaact gtggaaccag ggcttcattg caagattcaa aggatcggga tgtgtatctt
    23761 caaagaaggc ctcttcagga gaaccctctc ttatgctaaa tcgtttcaga atcaaggcca
    23821 atcttgacct tccgtaaaga ccttcaaaat agttattcat cgactctact gccctttgaa
    23881 aagaatagcc ctttagtcca tagaactcga gcgatatatc acggtacagg tcttcaagcg
    23941 ctgcaggaag ggatccagag aattccgcct ttggatcgag atcgagaatg tcagcctcaa
    24001 ttctatcttt aagaatgacc ttatcaatca ttgtctcaag ctgacgatat ttgtagccgt
    24061 ttcccgctcg tgctacgata tagtggttca cggtggcatc tagaaagtaa tggcaaatta
    24121 ctccaagcgt gtaaaagcgc gagatacccc cagctcgctc gcaaagtgcg attgcaaatt
    24181 cgtcgcacct gatagtatga gaaaacctcc agagcgaaaa ctccggatcg tctttggagc
    24241 aatatagtcc gggatccgta aagaggcatc caagattgaa ctcctctctt ttgtcggctt
    24301 caagatcgag tcctatctct tccagaacat cttttccgta catgatgtga gtccagaagc
    24361 tgggcaagaa aattcactcc ttctccacca aaccccacag acacttcaaa taaagactct
    24421 caggaacctc tacaagccaa ggatgatcag gcgattgcag cgtgatcgtc agatgagaaa
    24481 acgtaatacc agtgcgttcc gtcgatcttc ttacagactc taccaaatga tcgatgccaa
    24541 tattataggc gcatgtgcac agccccaaca cccccccatc cttcaccagt ggcagagtgc
    24601 cctctacaag tcttttgaaa agtgaaattc catgcggaag ctcttttctg tgcttaacaa
    24661 aggatggcgg gtccactact actatatcaa aataatcctt cttttcaaat ccagcaagga
    24721 agtcaagaac atcgctctga acgaactgca gcttattatc cagcttgttc actaccgagt
    24781 tcattcttcc ctgctctagg tcgtctcccg atttgtccac acagattgca tcggctccag
    24841 aacacgccat atttagacca aaacctcctg tgaaggaaaa caggtcgagg cctttcttta
    24901 gagacagttc ttccgttatt ttcctgcaga acgttctcga gtctctctga tcatagaaaa
    24961 aaccagtctt ctggctattc ttgacatcta caagatactt caggccgttt tcctcgattt
    25021 cgattgaatc cggtacctca ccaaagatca ggcccgtgtg tctctcaatc ttctcgtcga
    25081 ctcccatttc gaagtcactg cgttcgtaga ttccattagg tttaacaatg tccctgagga
    25141 gtctcaaaat catttccctt ttttgctgaa tgataagatt tctgaactgt attgcaataa
    25201 tctctccata tcgatcgatt accagtccag gaagcctgtc gccctcaccg tgaacaagtc
    25261 tgtaaaaagg tctgctgaaa agcctttctc tcttccgtaa ggcattgctc aacagacgtc
    25321 caaagaagcc ttctccaagc tcctcatcct tattagtgag aaccatgatt gcccgacttg
    25381 aaaagggact gaagaatccc tttcccagga attcataacc ggccgagaac acattgactt
    25441 cgcatacttt gtctgttggg ccttcgagtg acatgatttc gtccttgaag atccaaggat
    25501 aagaattaag gacctttccc tcctttcctt tttttattcg agcaattagc attaaagctg
    25561 gccttcaatc acgagtgagt aatcgttgat ctcactttca tcaaagaata tccttgcctc
    25621 tcgctcggca ctctcgacag aatccgaagc atgaatcagg ttctttcgaa ctgaaacacc
    25681 aaacgaacct ctaatgcttc cggggtatgc ctctgtcgga tcggtctttc cgaccaaaac
    25741 tctaagtctt tttacggcat cttcaccttc tagaatcatc gccaccacag ggccggactg
    25801 cacgaacgcc agcaggggtt cataaaactc ctttccggcg tgttctcgat acaattctct
    25861 tgctctcgat tctgagatcc tgagcatttt cagggcgacg attttaaagc ccttttcttc
    25921 gattctcctt attatttcgc cgacaagacc tcgctgtatc gagtttggct ttagatatgc
    25981 aaatgttctc tccacaatac ttccctccgc tcaaaaacta tattcgttgc tataattaaa
    26041 acatgcagtg gttcgatgga gtagttaacg atagactttc ccgactcttg acgcgcagtt
    26101 cgggcataag tattactttg gtgggcaggg acagcgaata cctgaaagtc ctggcaacgt
    26161 cgatagttga aggcgatccg aaatggagta aaaggcgata cgtagaagat tttcttctga
    26221 tcgagcctga aagcggaaac ataggcatcg atagaattcg cgacatagaa gaaatcatgc
    26281 ttcatagacc gtcgggagac gggaagaagt acgtgctcat tcatcaatgt gaaaggatga
    26341 cagatgagag tgctaacgct tttctgaaaa tccttgaaga gcctcccagt tttgcaaccc
    26401 tcatcatgac gacaacctcc tggcagtccc ttttgccgac aattaggagc agaaccgttt
    26461 cgatgagggg cgaggttccg agagaattga tggatgaact cagagcaaag tacggaaaac
    26521 gtgttccgga aatctttgca gcttcgaggg agaacttcac catcttaaga tactttctag
    26581 agcagaatcc cgattcagtc gagtccgctt cttttgaggt tccttcagat cttgacggtc
    26641 tggttgaact gattaaggag gatgtctcgg agagtttcga atcgattgtg agaaaaagaa
    26701 gagcatttgt agctctcagt aaaaatttca tatcgactga cagttttttc tcgactttca
    26761 gacgggtttc ctctgtcttc accggctccg gatcattcga gagggctcgg gaattagtga
    26821 aggttgtcag aagcattttg agcgacgcgg ttattgcttc agtaaatccc agcagtaagt
    26881 cggttactaa ccttgatctg atagaatggc tagtagactt cgatttggga aaggaaatta
    26941 tgacggattt catttggtgc gacaggtttc tgagacagag aacatcttca ctgagttcta
    27001 cgttagttgc tttaagagct ctaaattcgc tttccagaaa ccttttgaga agagaaaacg
    27061 gaggtaattg agatgcctga gatatatgga acggtgtacg gagtggaatt ccaccctgtt
    27121 gggaaactct atcagtacac atcgagtgag ggactgttga agcagggcga ctatgtattg
    27181 gcaattagcg aattcggact cgatgtaggc aaggtgatgt acggccctgt tgaaatcagg
    27241 attgatgatt cgaaggaaga gctgaagccg attgtcagag ttatgactga cgaggactgg
    27301 gaaactgact ctaagaataa agaagaggca aaagaggcaa tgaagacttg tcaggaactg
    27361 atagagaaac actcgcttcc aatgagactg ttagaggcga gatacatgtt tgaccgttcg
    27421 agaatcgtct tttattttgg cgccgacagc agggttgact tcagagaact cgtgaaggac
    27481 cttgcgcggg cgttcagaac gagaatagaa ctgagacagg taggtatacg ggacgaggtt
    27541 aagatgaagg gtagccttgg gctttgcggt atgacggctt gctgcgttcg tttcctcagg
    27601 cagtttgaaa gcataactct caagcatgcg aaaaagcagc agttgttgat aaatcctgca
    27661 aagatctctg gtagatgtgg aaggctgctt tgctgccttt cgtacgaaca ggaactttac
    27721 gagaatgagc tcaaagatat tccggatgag ggttctcttg ttgattatga aggaaagacc
    27781 tgcaaggtct tgacagtcaa tattttcatg aagatcatta ctcttgtgtc cgatgacgga
    27841 cagatgttga aggttcagtt tgaggatttc agaaagagtc agagagcaat cattcaggac
    27901 acaaaccctg atgagcttat aaagaacagg gatgaagata tttctattga cgactgaagg
    27961 atgaatggag gttggatatg gcgggattac gaacgagaga agaattgaaa agacttgaag
    28021 atcttggaag gatatgcaga ggtgacatca taaaaatgac cacggtagca aactcaggtc
    28081 atcccggtgg ttcgatgtca tcgatagaca tcttcctttc gatctatgat tttgcaaaga
    28141 atgatccgaa taaaccattt gatcctgaac gcgacagggt tattgtaagt cacggtcaca
    28201 catcgccggg tgtttactcg accctcggaa ggctgggctt tgttgatatc gatgaagtaa
    28261 ttgctggctt cagacatcca ggctcgatat ttgagggtca cataacaagg ggaattcctg
    28321 gagtagaatg gacgactgga aatctcgggc agggactgtc ggtcggtgtc ggtatggcct
    28381 tagcatcaaa gctgagtggt aagaacaata gagtctttgt tgcaatgagc gatgccgagc
    28441 aggcgaaggg tcaggttgga gaggccagaa gaactgccgt gaagtgcggg cttgacaatc
    28501 tggttgttgt catagattac aacgatgctc agatttcggg gagagcccac aacattatgt
    28561 atgtcgacat taaggccaat tatcttgccg atggatggaa ggtaattgag gtggacggcc
    28621 acgactatct ggaactgaac gaagctcttg tcgaggctga agattcaaag gggcccgtgg
    28681 tagttattgg aaagacaatc atgggaaagg gagtttcctt catggaaggc gatgtttctt
    28741 accatgggaa acctctggac tttgaaaaag ccgctttagc tcttgccgaa ctgggtctag
    28801 agaacgatat cgatagattc gttcaaatga gaaaagttct tccttcttat cacgagccgg
    28861 ttcaggcaat agacgaagat gtcaaacctt tcacggggga tcctatagtc tatccagttg
    28921 aaaagaaaac cgacaataga tctgctttcg gaaaggcggt tggagatatt ggaagactga
    28981 acaaaggcaa acttccagtg atggttgtag actgtgacct gaaaccttca acgaaagtga
    29041 gcgagtttga aaaagtctgg cctgaaaact tcgttcagat tggtgttcag gaacataacg
    29101 cagcgacatt ggccggtgcc gcttcggtgt gtggtgtcct aaccttcttc gcagactttg
    29161 gagtgttcgg tgtcgatgag acttataatc agcagcgcct aaacgatata aacaaggcaa
    29221 acgtaaaagt tggagtgact cacgtgggaa tcgacgtggg tgaagacgga aagactcacc
    29281 actgtcttga ttacatagga gctctaagaa atttctacgg cttcaaactc atagtgcctg
    29341 ccgatccaaa tcaaactgac aaagcggtaa gattcatgag cgagacgaaa gggaactttg
    29401 ttatcgccat gggaagatct acaatgaacc cagtggccga tgaaaatgga aatcctttct
    29461 tcggagagga atataaattc gaatatggaa aggtcgatgt aataagaaag ggaaaggata
    29521 ttaccctcgt cacgacgggt caggtgactc accacgcagt taaagctgcc gacgagctta
    29581 gatctcacgg aatagaagtc gctgttctaa acgtgagctg tcctcttgga gcaaattttg
    29641 agagcgtaaa ggagttcttc tcctctattg ttatttcttt agaagaccac aatgtggcaa
    29701 gcggtctcgg ttcaatcctc gctgactacc tagtaggctc tggaattata ccgaaaagtt
    29761 tcgagaagat cggtgtcgag agatacatgt tctccggaga caacgaactg ctttatgatc
    29821 tcgcaggttt gtccagcaaa gcaatagtcg aaaggatcag gggtatcatt aaataatgct
    29881 aagcgctgaa gaagaaaagc tgatagaaaa gttttgcaga atcaatggaa ttgaagcaga
    29941 cagagagctc gtttttaggg ctctctgtca ttcctcattc acaaatgagt tagcccagaa
    30001 tggggagaga acccttgaat cgaatgaaag aatggagttt ctcggagacg ccgttctcga
    30061 actctccctt gccaagactc tctacaatga gtattatctg actgaaggag agatgtcgaa
    30121 aataagggca atggtaggaa gtgagaaggt tctctccagc gttgcgaaga ccatgagaat
    30181 cggcgacttc atatttctgg gaaaggggga aaggcaaacc ggaggggcag aacgagactc
    30241 tattctggca gacgcctttg aagcggtcct tgctgcaatc ttcctgactg gaggtctcgg
    30301 ggtttcggtg gagttcgttc aatcgaaact ttcggattac atcgagagag ccgtagaagg
    30361 agagctgatt cttgattaca aaacctctct tcaagagttg acccaggctc gctttggttc
    30421 aaggcctttg tatgagactc tcctcgacga aggtccgccg caggagaaat ggttcaaagt
    30481 cggtgtcttt cttgatggga gtttaatggg cgaaggagag gggcgcacga aaaaggctgc
    30541 cgaacaactg gcagcaaagc acgcccttga gattctgaaa aagtcggggg cgaagaaatg
    30601 agatacagac acttgattgc ttttgtaacg cccgaggaaa gtgattcgat cgaggggatg
    30661 agctggaacg aaggcttcac aaatctatac ttcgaggaaa tcgaaggttc tggaatctca
    30721 ttgcacgttc ttcttgaaga aggagaggaa gtcccggctt ttcttcgcga gatagagttt
    30781 tcagatctcg gcttcagcga ggagagagat tggtttcaaa agtggaagga gaccttagtc
    30841 ccgttcaact tgtgcgacgg gttcgaggtc attcccctgg aagaagaaaa gatggtcggt
    30901 tcaggaaaaa tcggaatcat tccgggaatg gcctttggta ccgggttaca tgaatctacg
    30961 agacttgccg ccgctattct caatgaaacc gtgagagagg gtttaaaagt gctcgatgtt
    31021 ggctgtggaa ccggtattct ctcagtgatt gctgccagga aaggtgctgc agctgtagtt
    31081 gccctcgata tagacgagca ctctatcgaa aagacaatcg aaaccgcgcg aatcaacgag
    31141 gtctcgatag aagcaagact gtcggatttt ctttctgcag tgaagccggg agagcgtttt
    31201 gatctcatag tctcaaatat gatcgctgaa ttgttgatga aatttgtttt ggatcttcca
    31261 aagtttctta ctgaggatgg gatggtaatc ctttcgggga tctataaaga caagtatcgg
    31321 gaaatagaga gcctcgcaga agaggagttt tcgattgaga acgtcaggga agatggagat
    31381 tggaaagcaa taacattgaa aagaaaatag tttcgcttca actcggaaga gctgtgaaga
    31441 atgattttat agttgccagg ctttgcgatt ggggctttcc tcaagtaatc gagtcctcgc
    31501 ttatctctga tggcaggcct tttccaaccc tgttctggct tacctgccct ttcttgaaag
    31561 gagaggtttc caggcttgag agtaaaggaa tgatctctca tctcgagaag aggattgagc
    31621 aggacaaatc cttcgcgacc gcttttctcg gtgcacatcg tgagacaaca ctgcttaaag
    31681 agaagatact ctgtgaagcc tctgtgtcgg gggaaagccg aagactcctt cttgatagag
    31741 gtatcggggg aatcagaaat ctgaagaaag tgaagtgtct tcaccttcac cttgcgaact
    31801 accttggtgg gatcgataac cccgtgggaa gagaagtctg gaatcttcta gagaagaaac
    31861 agtgttcttc aactaaaata atctgcagag agttgctgag agagaatgaa tgaagcgata
    31921 ctcaacaaag ccagtcagct tccggaggaa ccgggagtct acatattcaa gaacgagaaa
    31981 gatgacgtcg tctacgttgg caaggccaaa aaactgaaaa ggagagtcct ctcatacttc
    32041 agagaatcaa cctggtcaaa gaatgaaaag gcaaggtgta ttgcagaaga ggcggaagat
    32101 ctcgacttca taatggttac gtcggagcga gaagctctcc ttctcgaagc aaatctgatc
    32161 ttcaacaaga agccgaaatt caacgtcttg ctcagagatt cgcgtaccta tccctatatt
    32221 tacatttcgg ctgatcaata cccatatcta gcgattacga ggacaaaaga gcttgaaggt
    32281 tcttatttcg gtccgtatac cagcgcaggc cttgtaagaa agctgcttga atttctccag
    32341 aaagtcttca aaatccgtac gtgtacttat gaacttagca gagtcaagaa accatgcttt
    32401 ctctatcact tgaagatgtg ttcggcgccc tgtgtagaga aagtctctcc cgaggagtat
    32461 cgagaacagc tttctgcgtt gatcgagttt ctagaaggag acacattgaa ggtaagggaa
    32521 gcactcttga agagaatggc ggtgctctct gaggcgctgc agtttgagag agctgcggaa
    32581 ataagggata ttctctcgac aatggacgat ctttatgcct tccagggtgt tgaagcccca
    32641 ctggatctta aggcagatgt tctggccgtt tcggccggac tggcagcgtt gcttcaagtg
    32701 aggggcggca tgctactagg aaaactgatt ttcgattttc cagagggaac tccgatagat
    32761 ttcatcacgc agttctacta cgccaaggga aacaggatgc caaaggcctt gatcgtaact
    32821 ggattgaaga agaaggacat aaagcaattc aaaagggatt tcccgtatat tggggagccc
    32881 cgtgatgaac aggaagaaag gctgctgagc atcgcattca agaatatcga cgaagaaatg
    32941 aagataagac taaatgcgac acattcgctt aagcaggccc agcaaatact gggcttgaag
    33001 cggtttcctt caagaataga gggaatagac atctctcaca cacagggact ctacacagtc
    33061 gcttctgtag tcgtttttga taacggtaaa cccaacaagt cggaatacag aagatacagg
    33121 ataagcgaac tggaagaacc aaacgatttc gaagcgatgg cgacggttgt gaagagaaga
    33181 tatacaaagc acccgcttcc cgaccttctc ctgatcgacg gaggcgagcc acagcttaga
    33241 gcagtggaaa aagcgtttgc agagataggc attcaagaat atgaaattgt tggtctcgca
    33301 aaggagttca atgagcttgt ttttttagat aacagggaca gagtgaagct gaaagaagag
    33361 catcctgttt tgagaatgat aatttccata gacgatgaag cccatcgttt tgctatcaac
    33421 taccataggg tactcagaga aagaagattt cagagctcaa agattgatga cattccggga
    33481 atcggtccaa aaaggaagaa ggcgctaatg agggcgttcg gaagcgtcaa gggcattgca
    33541 gaagcttctg aggccgatct ttactcggtt ctcagaaaca gcaaagccgt tgaagccgtt
    33601 actaaatggg caaatgaaaa aagtggagac tgagctccac ttttctctat tggttaggat
    33661 aattgctatt gtactttctc tttaagttct tttccgggtc taaatttcgg aaccttgccg
    33721 ccaggaattt cgattgcttc ctttgttctc gggttaactc cctttcttgc ctttctcttg
    33781 gtaacctcga atgtcccgaa accgacaagt ttcacttctt catttttcga tagctcttct
    33841 ccaacgacat cgatgaaacc gtctattacg tctccaacga gtttcttggt tgctccagtc
    33901 ttttccgcga gcacagcaat aagttccttc ttattcacag gtacaccccc tagctagaat
    33961 tcacatcaca aaacaattct aacattttct acttgattat tgcaacagcc gatccaccga
    34021 aaccgtttaa caaaaggact ttcaaatagt tactgttcta cgatgctcga ttcgcgcagc
    34081 ttatcgaagt tgagaacgtc tctgtgcgcc tcgagtgtgc ttgtaaatga ggacaaatca
    34141 agcgcgccat ttccgctaag tgtaaataca attactttct cctctccagt ttctcgggct
    34201 tcgattgctt ttcgcacggc accggcaatt gcgtgacatg attccggagc gggaatgatt
    34261 ccttcggtcg ttgcgaagag aagtcctgcc ctgaaggtct cttcctgcgg gaaggcaatg
    34321 ggagtaacga ttccttcttt caccagtctt gagatgagag gcgctgcgcc gtggtatctc
    34381 agtccacctg catgaattgc gggtggaacg aattctcttc ctagcgtgta cattttaagt
    34441 aggggtgtga aaccggcaga gtctccactg tcgtacctgt actcaccttt tgagagtgag
    34501 ggacaggatt ccggttcgca tgcaatgaac tcgatatcat caccgttaaa tgttctcttt
    34561 acgaatggga ggatcgttcc gccaaagtta gatccaccgc cgtggcaacc aataatcgaa
    34621 gagggcttct ccccaagcat ttcaagctgc ttctcgactt ccagtccgat tactgtctga
    34681 tgaaggagaa cgtgatcgag aacgcttccc aaggagtact tcttcgtctt tgaattgagt
    34741 acgatttcaa ttgcctcact tattgccatt cccaatgagc cggagaagtt ctttctgtct
    34801 ttcaggaagt ttcttccgct ttcagtgttc tctcccggac ttggttccac ggtgccgtcg
    34861 aacattctca tcatgtgttg tctcattggt ttagactgaa agctgtttct gaccatgaag
    34921 actcttacgg tgagaccgaa cttccttccg gcgtaagaga gggcgctacc ccactgaccg
    34981 gctccggtct cggtagtgag ctcggtgatg ccatcggtct tgttgtaata ggcctgcgcg
    35041 atagccgtat tggttttgtg gctgccagtc ggggagtttc cttcatactt gtaatatatt
    35101 ctcgccgggg tgtttaagta ctcctcaaga aaggtagctc tgatgagtgg gctcgggcgg
    35161 aagaccgcat attcgtcgag aaccggttcg ggaattggaa tgaaccgctc ttcactcatc
    35221 tcttgttcta cgattgactt tgggaagatc gccatcatat cttcggggtt tataacttca
    35281 tttgttgccg gattcaaagg cggatccatc ttgaatggca aatcagcctt tatgttatac
    35341 cagcttctcg gcaactcctc tgacttcaaa ttcactacag ttctcttctt catgccacta
    35401 cctccttaaa ataaaagggg ctctccatca tgagagcccc ttcagtatgg gtttaccctg
    35461 gtagcaaaag gctaaccccc attgaagggg ctgctccacc accagaacat attcagttta
    35521 tttagatata cgctatcgat catacataat ccctcacaga atttcattaa tcaactatac
    35581 aacctaagta tgttacaagt caagtgatac ttcgaatttc gaacaatggg attttaatga
    35641 cgataagtta taaatcatag ggaagcgatt ggaagtatta ttgagcatat gaaaagagcg
    35701 ccgaatactt gtttttgttt ctacaaccac caaatttctc tcggttcaga gaaaaacgtt
    35761 ttggtccaga gatattgccc tttcctgctt ttttcttgtt tcagatttcc aatatggtga
    35821 aagcaatttc gcggcagaca gtagggtgtg tagataacgc atctttaatt ggcatctaat
    35881 gtgctagatt gtgacatata gatgctcttt ttgttaaatg aaaggatatc ccatcatgaa
    35941 aaactttgtc tcggcagcca ttcaatttga agcagaacct atgaacgtca aggtaaatct
    36001 tcaaaaagct tatcacttga taggcaattg caaaagggag accggtgctt ccctcgtggt
    36061 tttgcccgaa agctttacta cggggttcac tccgattgga aacgcagagt ctctctggga
    36121 ggtagtagat tccatccccg gtagtcttac agatgaagct gtcagatggg ctcatgagtt
    36181 cgatacttac atatgctttc ccacttatga acgggggaaa gagcgcgctg tcatatacaa
    36241 tagcgcagcc atcgtcggtc ctgaggggat gattggtgta tataggaaga cccatccctt
    36301 ccctactgaa agactgtcgg cgggaggttg gacaactccg gggtcggatc ctcttgtggt
    36361 ttcggcgtcg ataggaaaaa taggtgtagt gatttgctac gatggggatt ttcctgaact
    36421 tgcaagggtt acggcactca agggggcaga agtcatctgt cgtccttctg cttttttgag
    36481 aaccttcgac caatgggaac tcactaatag ggccagagca tatgataatc atgtctactg
    36541 gattgcatct aatctagtcg gtaaggattc aagcgggtca aacttctttg gctcatcgat
    36601 gattgttcac ccgtcgggga tgaagctggc acaggcatct ggctgtgagg agtttgtttc
    36661 ggcgatgctt gaggccgatc ctataagaaa gataactccg ggcgcttcaa gagatcagat
    36721 atttgaccac atagaagata gaaaccttga ttcctacaag ggaatactca gcgaaggtaa
    36781 aagcgctttt cagccttcaa aaagaattcc atacaggagg agatgaactt gtcaaagccg
    36841 cttgaaggaa tttccgttct tgatcttacg cgggttcttg caggtccatt ctgcacaatg
    36901 cttctatgcg acatgggcgc gaacgttgtg aaggtagaac ggccggaagg aggcgacgac
    36961 tcgagatcgt tcagcccttt tgtgggtgat gacagcgcat acttcatgag cataaacaga
    37021 ggaaagaaaa gcattacatt gaatctcaag accgttgaag gaaagaagct ccttgaaagg
    37081 cttattgaga ggttcgacgt acttgtggag aacttcagac cgggtgtatt cgagagactt
    37141 ggtttttcgg cgcaaagact gcgtgaaatc aaccccaggc taatctacac atgttcatct
    37201 gggtttgggc actccggtcc catgagtcat agaactgcct acgatcttat cattcagggc
    37261 ctgagtggaa tgatgagcat aacaggtccc gacgaaaaca gtccgacaaa ggtcggaagt
    37321 tccattgcag acatcttctg cggaaccttc gcctgtatcg gaattctcgc cgctctccac
    37381 agccgtaatc tcaccggcaa aggacagaag gtagacgtgg caatgcttga cagcatggtc
    37441 tccgttttgg aaaatgccat cgcaaggtac gccgcgaccg gcgagccgcc cgttccgata
    37501 ggaaataggc acccttcaat cgcgcctttt gcatctttca agacgcttga tgggattatc
    37561 aatatcgcag ttgggaacga cgtcttatgg gagaaatttt gtaaggcaat tggaagaccg
    37621 gaaatgtgtg acgacccgaa attcgtcaat aatcctgcaa gagtaagaaa cctcgaagaa
    37681 cttatggaaa tccttggagg agaactagaa aagagaacct cagacgaatg gctgggtgtc
    37741 ttcgggaagc ttgatattcc aagtggaaag attaacaata tcgccgacat atttgaagac
    37801 gagcaaatca gagcgaggga aatgatcgtt gaagttcccc acagatgtgg aaatgtcaag
    37861 atgccgggtg taccgataaa gttctcggat acgccggcct cgattgccgg gccagcccct
    37921 ctcttagggg aacacaaccg ggaggtttat gctgggatgc tggggcttag cgatctggaa
    37981 atggaagagc tgaagagaaa aggcattata tgatttacct ctatccttac gaaagaagtt
    38041 cagactggca agagagtaca tatagactca cagtgaagac ggcattcagc aaatctgtga
    38101 acttcagaga cgaacaaggg agaagatact cacttctagt cgatgatgac gatttcctgc
    38161 cacgatcggc attgatcgag ggacttcctc caatggcaag cggtcacgaa gttggaatcg
    38221 atataaaagg cgccgtagtg aaattcgatc cttcagccat gagcggactg ccggatagaa
    38281 agatgagggg attgtggctt gaatggctaa gcttaatgga caatgctgaa ctcaaatgca
    38341 ttcaggaaaa ccttgatgaa ccattcagac tggtgggtct tgggccgggc ctcacgccgg
    38401 cgggagatga ttttcttgtc ggatggatta cggcctgtaa gttggaaggg agaaacacaa
    38461 agaagttgtc aagtgagata gaggaagcac tcagtcgaaa aaccacctgg ttttcgtccg
    38521 aaatgatcag ggatgcactt gacggaaaat tctggaagcg aggaatagaa cttgcactag
    38581 cgctcaatga aggcgactcc tttcaagtca tgaaaagggc aggaaaaatc attaactggg
    38641 gacactcctc tggaaaagca tggcttgcgg gtttcgctta cggaattgag agaggaagtg
    38701 atccacagct tatctgagta gatctcaatg tcttaaatga gagttattat gtagggtcta
    38761 tcgaattgtc agagtgaata ttaaacattt gataagtaat tattactaga actgccgata
    38821 tcgtttctgg aggttcatat gaagaaaatc gaggtcgtta gaggcgaata ctacgatagc
    38881 gtaacgttga tgctcgtggc caaggaaatt aagagaatta atggcgtcgt agatgcggta
    38941 cttaacatgg cgacggaggc gaacattgac atcatgaaag ctgccggctt tgaaatcaaa
    39001 gcggggtttc tttctccgga tgacctgtta atcggaatcg actacagaaa agacgaaata
    39061 gataagatct tcgaaacagc tcgttcgtat ctcgcatctc ctccttggaa gaagaaagaa
    39121 gatgacaccg agtatactcc gggaacgctt gaaggtgctc tcgctgtgtt gagagacgcc
    39181 aatcttgcct tgatatcgct tcccggaaga ttcgcagcag ccgaggcgat gaaggctctc
    39241 aagaacggct tgaacgtaat gctttactca gacaatgtcg caactgaaga tgagataaag
    39301 ctgaagcatt acgctgacag aaacgacctg atcgtcatgg gaccggactg tggcacagcg
    39361 gtcatcaatg gaaagggcct tggcttttca aatgtatgtc ctgagggtcc tgttggaatc
    39421 gttgcggctt ctggtaccgg ccttcaggaa gtaatggtgc agcttagtag gcgcggcatc
    39481 ggtgttaagc atgggatcgg aacgggaggc agagacgtta aagaatgcgt cggagggata
    39541 tctttcctca aaggaatacg agaactctct gatgatccag agatctctct gattattgcg
    39601 gttggaaaac cgcctgatcc tgaggttaaa gagaggattt taaactcttt gaagaagact
    39661 ggaaaggatt gtgttatttg ctttatgggg gatgattgcg ggaaagacga agatagagtg
    39721 cattattcgg cagagctcga ggaatgtgcc gtcgttgcag aggcgattct taatggcaaa
    39781 gaggtgtcag ctgcgaggga caagttgaga gccgagtacg ctagcctttc aggctggagg
    39841 cccattgaag aggttcaggg gaaatatgtc aggggtcttt tcaccggtgg cactctctgc
    39901 tacgaagccc agtacatcgc acagtcttac atcaacccta tttacagcaa tgcgcctcta
    39961 aggaaggaat tcaagctaga gaacagtctg aaacccatag gtcattcgtt catagattac
    40021 ggagaagacg agttcactca ggggagactg catcccatga tagatccttc attcagggcc
    40081 gagcagctta aggagcagtc ggaagataat tcggtggctg ttgttctctt tgatgtagtc
    40141 ctgggatatg ggagtgcgga aaacccatct caagaagttg cagaggctat cggagcggta
    40201 aaaagagaaa ttaagccggt ttatgttgct tatgtttgcg gtacagcggg agaccctcaa
    40261 gacctggcga aacagatttc tcttttggaa gatgcaggtg tcatagtctg tgagagtaat
    40321 gccagggccg cgcttctggc ctcgagtctc atttcaagaa gggagcgata atcttgtcgt
    40381 tcaaagattt gctaaacagt ggtccaaaag taataaacct tgggatggag agattctatc
    40441 tcgatcttct ggagcaagag gttccggcgg tcaaggtcaa ttggagacct cctcttgcca
    40501 aaagttcgtt gatggataga ttgaggaaat tgagaggtga agaggttgaa tgacatcgat
    40561 aaagcaaatt ctatagcgtt tgaaaggctt ttgaaggccg aaccccagat tgttggtctc
    40621 ggaatggcca aagagtttat tccaaatttc ggagaaaaaa tgattcttca tgccggtccg
    40681 cccataactt gggagaggat gtcgggccct atgataggcg ccataacagg cgcaattctc
    40741 tacgaaggtt gggcggaaac tccggaaaag gcgagagcgt tggcttcgaa aggagaaatc
    40801 acttttgaac cctgccatca ccacaactcg gtcggcccta tggcaggaat aatatctcct
    40861 aatctgccgg tatgggtagt tgaagaaaag aattctggaa ggagaggcta tgcgactctt
    40921 aacgagggcc tcggaaaggt cttgcgaatg ggtgcattca gcgaagaggt catagaaaga
    40981 ctgaactgga tgcgtgaagt tctttatcca gtgttgagaa atggggtgcg aaaatacgtc
    41041 gaagataatg gtggcctcaa tcttaagagc ttgatctctc agtctctcca catgggagat
    41101 gaactccata atcgcaacag ggcagccact tcacttctga ttagaacact tgttccttct
    41161 ttgatagacg cggaacgggg taatcacaac ctctccaggg ttgtgaagtt tatggcaggc
    41221 aatgatcact tcttcttgaa tcctggaatg gccgcggcaa aggcctcgct tgacagcgtc
    41281 ggttatgtag agggttccag catgatcaca atcatggctc gaaacggcac cgacttcgga
    41341 atacagattg ccggaataga gaacgagtgg tttacctgtc aggcggctct gcccgatgtc
    41401 ctgctatttc ctggtttcac gaaagacgat gtaaatcgag acataggaga cagcgcaatt
    41461 atggagactt acggagttgg cggatttgca ttggccgcag cgcctgcgat cgttcagttc
    41521 gtcggaggca cccctcagga cgcggttaac tacacgaagg aaatgtatga gatctgtgtg
    41581 ggagaaaaca agcatttcac gattcccgta ctgaactttc gcggaactcc tacgggaatt
    41641 gatttgatca aagtcgtgga aacgggcatc actccaatcc tcgacaccgg tgcggctcac
    41701 aaagaggccg gcaaaggaca ggtcggagcg ggaatagtaa ggatgccgtc tgaagccttt
    41761 gtcaaggcag ctgaagcatt cgttgagaaa tattccaaat agaggaggta gtttatggta
    41821 ccgaagaact gggacaagat aaggatttac gagttatcgc aaccgatcag ccacctaaca
    41881 ccaccatggc caacctatga gcccctacag ataaagtttt tcaagaggct tgccccaaac
    41941 ggtgcaaacg gacagcttct aacgcattcc aaccatgttg ggactcatct ggacgggtcg
    42001 ttgcatttct gcactcacgg gagagacatc gcgagcatac ctctcaatga acttgttgcg
    42061 cccggagtaa tagttgatct ttcggatatc gcagaagact atggaatcta cacttccaag
    42121 gacatcgaag agagagtaga ggtgagagaa ggcgatattc ttattatcaa cactggatac
    42181 cacaaatatg cttacgatca gcccgaggcc gacgaagtaa gatacatgat aaaacacccc
    42241 ggaccaacca gggaattcgc agagtggtgc aaaatgaaga agatcaagtg gataggtgtc
    42301 gactgtggtt cggccgacca tccaatgaac acgaagattc gtgagtggat gccggttcag
    42361 gcaaaggagt gcgaccgcta tatgcatgag aaatacggga agtcactcga agagatcttt
    42421 cctgacgaag actaccagtt gatgcatgtt cttctcttcc catatgacat tattcacgca
    42481 gaaaacgtcg gcggagagat agacaagata cttaacaaaa gaatggtaat aggttgcttc
    42541 ccctggcggt tcgtcggcgg agagtcgtgt ataagcagaa ttgtagcttt cgacgaagag
    42601 gaataaatct tattgaaagt gtggcgatag cgaatgcgtg acttcgttct agaaagaccc
    42661 cgctcgattt cggaggtact ggacacgctt cagcgccatg ggaataaggc catggttaag
    42721 agcggtggca ctgacgttat tgtatggctg cataagtcta ttaaggaacc cgactatatc
    42781 gttgacttga cggctgtgga gcaactcaaa ggaatagttg tgaacgagga caacgttgaa
    42841 gtgaaagcgc tggtaactct caacgaagtt atcggagacg attcattgaa tcgttacttc
    42901 ccagctttgg ttcaggcatg caaggcacac tcggatccac ttataaggaa tcgtgccaca
    42961 gttgtcggaa atgtctgtgc cgcagtccct tcaggagata tgatcgcacc tctggtttgc
    43021 tatgacgcga aagtcgagat cacgggaccg aacggcagtc gaaaggttcc ggtctcggag
    43081 tttattactg gaccaaagag aacctctctt ggaaccgatg agatcgtcac aggaataagg
    43141 atcgctcttc cagcagtgga gaccagcggc tgttacctca aagcaataag aagagaggct
    43201 ctagatatcg ctcaggctgc cgtctgttgc acgctcttca aggatgataa cagagagttc
    43261 agaattgctt tctgcgccgt ttctgctaga ccccttagag cgactgaggc tgaaaagctg
    43321 cttaactcct gcagcaacat agatgacgag gtaatagacc aagctgcaga attagcggcc
    43381 gatgcagtcg atcccatcac ggatgtgaga gcctccagag aatacagggt cgagatgatt
    43441 gccgaactga cgagaagagc aatctctcgt tgtcttgaaa gacctgcggg agaaggtgtt
    43501 cacaaatgaa ggacaagcgc gtaaccatta cgctcaacgg ccgtaagaca actctggttg
    43561 ttgaagatag aacgacactt ctaaatgccc taagagagca aggcgttacc agtctcaaga
    43621 ggggttgtga agagggagaa tgtggagcct gtactttgat aatgaatggc cttccccaaa
    43681 agtcatgcct tattcttgcc agagaagccg agggtgcaga cattctcacc gtcgaaggac
    43741 ttgtgaagaa aggaagacta catccaattc agcaggcctt catcgatgaa ggcgccatcc
    43801 agtgtggata ctgcactcct ggaatggtaa tgactactta cgcactgctg aagaaaaatc
    43861 cttctccttc agatgaagag attagggaag ccctctcggg aaacatttgc aggtgcacgg
    43921 gttaccttgc aatcatcaga gcggtcaaaa agagtgccgc cattctttct ggaaataacg
    43981 gaggaggtgg gacgcgtgaa gactattaag acgaagactg ggatagggaa atggctgccg
    44041 aggaagtatg acttggaaaa ggccgaaggt acacttcagt ttgccgacga cctgaggttc
    44101 ggctccgaac tgcttcacgc agcggttgtc cgttcctctg tggctcatgg agagatcctg
    44161 aacgtcgatg actcggaagc tctgaaaatt cctggagtgt tgaaggtcct tttaggagag
    44221 gatttccccc atcactttgg actatatttg aaggacagaa cccccatggc cattggaaga
    44281 gcgagatatg ttggagaacc cgtcgctctt gtagttgccg agaccgaaga ggcagctgca
    44341 gaagcagcga gaaaagtcaa agtaacttac agcgaactcg aagcgatctt tgaccccgtg
    44401 aaggcagcga cgaataattc agtattggtt catccggatc ttcataagta cgagcatgtt
    44461 gatttcataa cacctcagcc gaacaccaat atcgcaaact ggttcagaat tagaaagggg
    44521 gacacggagg cagcattcaa gaattctgct catgtcttcg aggaaacaat aacctgccct
    44581 cagatcgctc acggtttcat tgaaactttc tgtacgatat gcaaacagga tcctgggtct
    44641 ggagagatca cgatctggac aagcgcgcag tctcctttca cagtgagaga cattattgcc
    44701 aaaggtatta attatcctct tcataagatc cgagtgattg ctcctccgat aggcggaggc
    44761 tttggcggaa aggcaggaat gacagtcgag gctctctgtc ttgccgccgc gatggacgaa
    44821 gacatcaaag gaagacctgt gaaactcttc attccaagag aagaggttat gttgagctca
    44881 tgggttcgac agggtttcgt tgcaagaata gaaatggcgg taagcgacga tggattaatg
    44941 cagggaatcc gtaacacgtt ctggttcgat actggaatct cggccgaata tggtgcaaat
    45001 ccagtcagga gcgccgggta cacttcaatg ggagcataca acattcccaa cgtctgggca
    45061 gactgttatg cggtttacac aaacaaaccc tttggggggg cgtatagggg ctttggtttg
    45121 ccggagctta tgagtgctct ggaagtggtt gtggatatcg ttgcccacaa gctcaaaatc
    45181 gatccggtag agttcagaaa gaagaacctt attaagaagg gccagacaac ttgcacgggg
    45241 atgccaatgc acgatcacgc tcttgagaag atcgtcgaca aagtggtaga gaagatggat
    45301 ttgcacaaag aagaaccagc gacacggaag ggatggaaga gaggaaaggg gatcgcgctg
    45361 gcaataaaag cgcctgcgat tcctgcagac gcctcttcgt ccgctattgt caagctaaac
    45421 ggagacggga ccgtagaagt gctggctgcg acgatggata tggggcaggg tacttacagc
    45481 gccttcgcac agatcgtctc tgaagaactc gggattcctt acgactggat caaagtatat
    45541 tatccagata ctcagagcca cccttatgac tggcagacgg ttgctagcag atcctgttgg
    45601 tcaatgggaa tggctgtgaa aagagctgct agcgaattga gagagaagct cctcgctttg
    45661 ttcgcagagt attggcagac accggtcgaa acaataagca tagagtacgg agtcgtcaag
    45721 tgtcacaaac ttggcaagag tgaaaggata gacgaacgag ttcagaatgg tttcaaaatg
    45781 cctgacggaa ttttgaaagg tggcccgatt atcggcaccg gttcgtttac accgccggat
    45841 ctgatctatc ccgaccccga tacggggcag tcctcaaagg tagttgccca tttcaccctt
    45901 ggcgcagtcg ggatagacat tgaggtcgat cccggcacag gaaggatact tgtaaacaaa
    45961 gtctgcgcgg gttacgatgt cggcaaggca ataaccccga taaacattaa gggtcaaata
    46021 gaaggcggta ccgttcaggg tctttctgca tgccttatgg aaggcttgtc gtatgacgag
    46081 cgtggcagac tgctcaatcc cgatttcacg gactacaaaa tcgccacgat aatggatgct
    46141 cctacagaaa tcgagtcttt ctgggaagag gtacctgaag agatttctcc ttacgggaac
    46201 cggggtgttg gagagcattc gatgatagct ccggctccgg cacttaataa cgcgctgttc
    46261 aaagcgacgg gcatgaggat ccacagttat cccctcagca gagagagagt ccacaaagct
    46321 ttgaaagcaa aggaaaaagg tgagaccgat ttctgggaat ggccctatga actggatctt
    46381 gcatatatgc gtgcgaagaa ggagtggtga agaagccagc gatcataatt ttgctgtcaa
    46441 aaagagaaag aaattctctc aatttcacta ggtgtaactc ccgtcagcgg gtttctttgc
    46501 ttgacaagtc tctattgtga tgtatcatat tatctataag tgacggaaac attcgggaag
    46561 gagaagagct aatgttgaag acaacttcgg aaccggggat tacagattag aacgctcttc
    46621 agggacagac agactgtcct ttttcttcag tcgtacgctt catgttattt tttccttaaa
    46681 gcaaatattg tcgaaaacat tcataattgg actatatgat ctgacgaata cttcaagaca
    46741 ggaggaaaca aagtatgcca aggtttgaag atgtagacct caaacgtcct gcagcagaga
    46801 gagaagaaga gatactctcc tattggcgga aagatgatct tgcaactaga agtgtaacgg
    46861 aaagggaagg aagccccgag tttgttttct tcgagggtcc cccgactgcc aatggtatgc
    46921 ctggaattca tcatgttata tcaagaacat tgaaagatgc ggtctgccgt ttcaagacta
    46981 tgcaggggta ccaggtgaag cgaaaggcag gctgggatac tcatggtctt cccgtggaaa
    47041 tcgaagttga aaagcagctg ggcttctctt ctaagcagga gatagaggac tttggcatag
    47101 agaaattcaa tcagaagtgt aaggaatctg tatggaagta cgaaagccag tggaagaaat
    47161 tgactgaacg catagcttac tggatcgata tggaacatcc atacatcacc atggagaacg
    47221 attacatcga atcggtttgg tggattctca agcaatattt tgataagggc tatatctacg
    47281 aaggccacaa aattatgcct tactgcccga ggtgtggaac tccgctcgcc tctcacgagg
    47341 tagctcaggg ctacaaggat gagaccatag attcaatata tgtcaaattc cgccttaaag
    47401 gaaaagacaa cgaatacttc atcgtttgga ctacgactcc atggacactt ccatcaaacg
    47461 tcgctcttgc ggtaaatcct gagtttaact atgtcaaggt cgaagtaaaa gtagacggag
    47521 acgaggtcga atactactac cttgttaagg aaaggctgag cgcgcttctt ggcgaagaga
    47581 gcaattacag aatagtccaa gaactcaagg gtatggacct tgtaaatctt gaatacgagc
    47641 aattaatacc ctttgcaaaa gtagataaga aagccttcta cgtagtatat ggcgacttcg
    47701 tttcgctcga ggatggttcc ggcatcgtcc atatggcccc cgcttttgga gaggatgaca
    47761 acatcctggg aaggaaattc ggtcttccct tacttcagct tgtcgacaaa gaggggaaga
    47821 ttaccgaaga ggttactcca tgggctggaa tgtttgtaag agatgctgat cctttgatca
    47881 cccgatactt gaaagacagg aatctcttgt tcaaaaagga gcgtctcact cactcttatc
    47941 cattctgttg gagatgcgac actccgcttc tctattacgc aagaaaatcg tggtacataa
    48001 agaccaccga atatagagac aaaatgatag aagtgaacaa ctccgtcaac tggtatccaa
    48061 aatttgttgg ggaggggcgc tttggaaact ggctcgagaa catggtcgac tgggccctct
    48121 cgagagacag atattgggga acgcctctaa atgtatggaa gtgcgacgaa tgtgggagac
    48181 tgacagcggt aggctccaga aaggaactag tcgagagagc aatagaagac atttcggaag
    48241 atatagagct tcatcggccg tacgtagatg acgttcatct gagatgtgaa tgcggtggca
    48301 ggatgacgag aaccccggaa gtcattgact gctggtttga ttcaggcgcg atgccagtcg
    48361 cacagcatca ctacccgttt gaaaacaaag acaattttat gggcgatctt ttcccggccg
    48421 acttcatctg tgaaggaatt gatcagacca gaggttggtt ctattctctc atgtcgattt
    48481 cgaccttcct ttttgggcag tcaccgtaca agaacgttct ggtcaacaat ctcgttctcg
    48541 ataagaacgg gcagaagatg tcaaagtcca aaggcaacac ggtcgatccc tggaagatta
    48601 tcgaaaaata tggagccgat acgttgagat ggtatctact tgccgtctcg ccaccgtggg
    48661 tgccaaccaa gttcgacgaa gagggcgtaa aggatacgtt cagcaagttc tttggaacgc
    48721 ttcacagcgt cttctcattc tttacaatgt atgcaaatgt ggacaacgtc gacccgggag
    48781 aattcgatct tcccgtttcc gaaagagaag aagtggacag atggataata tccagactca
    48841 acactctcgt gagagaggta acagatcttc ttaaccatta tgatatgacg aaatctgtga
    48901 gggctattca agatttcgtg gttgaagagg tttcaaactg gtatgtcaga agaacacggg
    48961 acagatattg gacgagcgaa ctggacaaca gtaagaaggc agcttatcta accctgtacg
    49021 aagttcttct tacagtagcc aaacttatgg ctccaatcgc tcctttcacg gccgaggaga
    49081 ttttcaggaa ccttacgcat aaagagaagg aatcggtcca tcttgagttc tatcctgttg
    49141 ccgacgagag cttgatagag ccggatctcg agaagagaat ggctgccgtc atggatatcg
    49201 ttaccctggg tagggcatgc agaaacaagg tgcagatcaa ggtcagacag cctcttccga
    49261 agattctcgt tgacgggcgg ataaagaaga tcgttgaatc gatgcgcgag ttgattctgg
    49321 aagagatcaa cgtcaagaac atcgagtata ttgaagagct cggagactac gtcaattacg
    49381 aggtaaagcc aaacttcagg gttatgggcc ccaagttcgg taaagatctg aaagcgatcg
    49441 gaaatgccct aaggtctatg aagccggccg atgtagttgc cagggtcaag catgaaggaa
    49501 agatctcaat cgaggcagag gggaagactt tcgaactcac agaagaagat ctcgacataa
    49561 gaatccagga acttgaaggt ttcaccttcg aaatgagcaa tgaaaacttt gtcatacttg
    49621 acaccgagct cactccagag cttcttcaag aaggactcgc aagagagatg gtatcaaaga
    49681 tacagacaat gcggaaggaa gcggattttg agataacaga cagaatcaat gtctccttct
    49741 ctggcccgag ggaagtagtc gatgcgatcg atacttacag agaatacata aaagaagaaa
    49801 cactctccaa tgccgttgtc tttgaagaaa gtctcgactc tgggacagag tggaacttga
    49861 atggttatga cactttgatt agagttcaaa gagtttgatg cgatatgaga aaccgggagc
    49921 atctgctccc ggttttcctt ttatgcagaa cttatttaga ctgtcaactt aacaatgatc
    49981 gccggatcga gaaccctgaa ggtgaatgat tccgatatga agagttcgac ttctttgtca
    50041 gtgtgactct gataacctat ttcgaagtct ccgccgataa ccagttcaag gtcttcatga
    50101 tccttcggaa gaagcagtgc gtccttcaca actctgctta caacaatatc cgtgccgagg
    50161 aaggactcga ttcgcttcac aagcgggtat ccggaaacat gagaattgag aatcgccatt
    50221 gtttcgggat tgacgatgag agcgaacggt ccatccacga aattgtcctt caaaacctga
    50281 gcagcctttg aaagtgattc gatgatttcg ttcggctttg aacccagagg aatcggtttg
    50341 ttttcgaccg actctacgat tcctttgacg gccgcctttt catagccatt gtagattaca
    50401 ttttcctcga agagcgctat ctgttttgcc gcttcttcaa gcggtcccag atcggaatcc
    50461 tttgcgcctc gctctatatc gtccatctcc cagcggacca gcttgaaggg aactctcacc
    50521 tctaaaaggg gcttcgcctt tctaagacct acaccgacct ctccttcttt ttcgagaatt
    50581 ttgacccttc cttccggaac gaaagaatac tcccatccct ttggaccaac cacactaacc
    50641 acttttcttg cggagagata gctcttaaga acctgcttag ctctttcatt tatttcttcc
    50701 cacgcctcgc tcgtcaatgg ggctagttgt cttttgaaaa gatccattct aatccctccc
    50761 tattacatct ttccgatgtt cagaccgctg ttttcggaac ccgctccggc ttcctcaact
    50821 tcggtgattg gtgccttcgt gaacagatat gttttaagct cgtcatccca accgtccata
    50881 tttctacgga gccattcgag agtcatgcag gcatgctcta tctcctcgtc cctgttatgc
    50941 gccattacag ccttcagttc ggcatcttca gttacatcca ctctctggtt gtaccagtct
    51001 acggcctcga tctcttcctt aaggctgttg agagctctgg aaatgtcctt tgccttttcg
    51061 cttagctgtt caaacggttc atgataattg ctcatattcg tgcctccttg tcaagttaat
    51121 atgtttccca taccatcatt aactatttta tcatacttta aattgtaaca t
//
LOCUS       DAOQYI010000013        49904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_783_length_49904_cov_78.113603, whole genome shotgun sequence.
ACCESSION   DAOQYI010000013 DAOQYI010000000
VERSION     DAOQYI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 49904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49904
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_49904_cov_78.113603"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PKL93_05405"
     CDS             <1..865
                     /locus_tag="PKL93_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70733.1"
                     /translation="IENGERIDALVFYNSTSRGSAYGAQMVQNALASYSSFLLSEKLL
                     EHGLTIEDLNPVNVEKIDVAPEESQGTEILATLIPYLLLIYIFAGAMNIGLDTTAGEK
                     ERGSMPVLLVNQVSRSSIATGKILYVMTIAILNSIFTFIGLIIAFKLGGPAFGAENLN
                     LSSLSVSTLVGLFITLLTMSGLAAALIVLLGSIARNVKEGSGYVMPIYIMAIVLGIAT
                     MQMESPDNPLLYLIPLVNSIFVMKDIITVNFVTARFLLMLISNLAYVSIFIYFLTRVF
                     NSERIMDSSGS"
     gene            992..2272
                     /locus_tag="PKL93_05410"
     CDS             992..2272
                     /locus_tag="PKL93_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HNQ70734.1"
                     /translation="MKRIFSIGAFIFLIIVTLFGSEILADRYLQLLLRGNYHLAYEMQ
                     SVRARTLYTVDNMVQEFQQLEEEYGNYLFIFSTETSELRGYTVYIFHAQFERGFIDFN
                     VVVDDQGKIDTFVVQTTSQPGAIAEYIDTSKFDEFEITIGDGRYRLPAVITVPKEIDR
                     YPLVILIHDSGALDRDSTVGPNKPFRQIAWGLATQGVAVLRYDKRTFVFGERLSQTSP
                     SIETEVIEDVIKAITAASRIPSVSSIFLAGHGLGGRVAPTIAMRDSRVDGIILMATPS
                     RRELQVIIDKQKYISSLNKGEIGQQTQLLTDYLQIALEGKLPPGAPVLAATAGYYYEL
                     DRMNPIETIRELEIPVLIVQGDADFESTVQDYIMFMNALWTKINVYFQLLPGLDHYFM
                     PVEGEISTPDNYYEFRHVDGRLIDTLFSWIYIFD"
     gene            complement(2334..>2983)
                     /locus_tag="PKL93_05415"
     CDS             complement(2334..>2983)
                     /locus_tag="PKL93_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70735.1"
                     /translation="TVFDIIAEGLRVHSVGRSKEERKTMIAEILEKVGLRPEYMFRYP
                     HEFSGGQRQRIGIARSIVLNPSLVICDEPVSALDVSVQAQVINLLEDLKHDFGLTYLF
                     IAHDLAVVKHICDSISVMYLGKIVETAETDSLFNDPLHPYTKGLLSSIPIPNPHLRSV
                     RNREIVKGDIPSPVDPPETCRFVKRCPRAFDRCWKESPSLKEVSEGHFVSCFLYD"
     assembly_gap    2984..3083
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3133..4119)
                     /locus_tag="PKL93_05420"
     CDS             complement(3133..4119)
                     /locus_tag="PKL93_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70736.1"
                     /translation="MERILEVDNLKISFRTQLGKITPVDGVSFGLSKGETLGIVGESG
                     CGKTITAFSIIKLLPKNAFLGDKTKISFRGRDISTLSKEELQKIRGKSISMVFQEPMT
                     SLNPLYTIGWQISEVYRLHEGLDEDDAMKRSIEMLRLVGIPEPQKRVKEFPHQLSGGM
                     RQRVMIAMALACSPELLIADEPTTALDVTIQAQVLELMNELKSKFDTATIIITHDLGV
                     IAEMCDRVVVMYAGQIVESGGVFDIFDKPVHPYTKGLISSIPKIDVAKKDQKKLNVIN
                     GYVPHPSNFPEGCRFRPRCPVAFEKCLQQPPVVQIERQHSVRCWLFEGGDVL"
     gene            complement(4125..5057)
                     /locus_tag="PKL93_05425"
     CDS             complement(4125..5057)
                     /locus_tag="PKL93_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70737.1"
                     /translation="MSKFFYKRKLDEIEEKARPVHINSYKQLVWTKFKSHKLALFGAV
                     VLIGVAFFTLLGPLFVEVGYEDMDFSNLFSPPLTEGHPFGTDELGHDVLVRVMYGGRI
                     SLFVGFASAVITTLIGTLVGLFSGFYGGIVDRLLMRFVDVMLSIPMFPILITLTLVFG
                     SGIMNIILVLTVFGWMGVSRLVRGLVLSIRETEYVMSARAMGVRNGRIILRHVLPNVI
                     PIIIVSATLNMSYAILAESSLSYLGLGIQPPMPSWGNMLQRAMNYILGTTAGTNPWWL
                     TVFPGFFIFITVLNVNFLGDGLRDALDPKFVSES"
     gene            complement(5060..6046)
                     /locus_tag="PKL93_05430"
     CDS             complement(5060..6046)
                     /locus_tag="PKL93_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70738.1"
                     /translation="MKVYFLKRFLELVPIFFAISIIIFVIMNSMPGDPLLQMRMQNPR
                     AMTNDPERMKELREYYHLDDPLPVKYFTWLKSVLTGDLGYSSMYKSPVIDLIASRLPN
                     TLVLTITAWLIGLVFALPIGIISAVKKYSTFDYATTIFAFIGISLPGFWFALIAIIIF
                     SVNLGWFPVSGMATYGITGFWNIFSDRVRHLVLPAFVLGLVQVASWVRYIRTSLLEVL
                     DQDYVRTAYAKGVPDRKVIIKHALKNAMIPIITIIALDIPYFFGGALIIETVFSWPGM
                     GRLMYNAVISSDYNLAISCLMFLAIITLISNLVADFLYVMVDPRIRMGRKGA"
     gene            complement(6052..7419)
                     /locus_tag="PKL93_05435"
     CDS             complement(6052..7419)
                     /locus_tag="PKL93_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNQ70739.1"
                     /translation="MKFIELIDSVPDYKEFFTVEEMDRRSLELAKRYPGKVKVYEAGK
                     SRGGNPIYVLEIGSGKKNALLFGCPHPNEPIGAMMLDFLSEKLASDDTFRDQFDYTWH
                     LIKVIDVDGTKLNEGWFKDSSSIRKYAANFYRPPGHEQVEWTFPVDYETLHFHSPLPE
                     TQVLMKLIEEKKPEFMYSLHNSGFGGVYYYVSDAVPELYETFSKLPGLFDLPLSLGEP
                     EAPFLEQLEPAVFKLFGMAQEYDYLKKNLGPHKDPADVIKAGTSSDDFASSVADTFAL
                     VCEMPYYYDSRVEDMSEADITRKEAQRFNYQRSVENFEFVSEVMKAVEPYVIDRNSPF
                     YRVFKNVLETYPDQLQAQKNWIENDPSLERKASMAEVFDNKIVSQFYQSLMLGMLRRL
                     LRENDNGSKELAAISKIADEAFERKMEYLEDNLNYTAVPIKSLVSVQLIAGLNVADYI
                     QRRES"
     gene            complement(7485..9572)
                     /locus_tag="PKL93_05440"
     CDS             complement(7485..9572)
                     /locus_tag="PKL93_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ70740.1"
                     /translation="MKKLFVCMLVLFFSVLSLAQISAEEAIPVIESRGILTSVDESPL
                     TFTEFKAAIEKAFPGKEEIIKGKGEVSRADFAVAMVEVLGLQSEAEAFDEICTTALDE
                     WEAPEEAWGALTVAYRSNRQLLDFRYGHVIEASSPITRKEAAISIYMAMNPPAKGGTA
                     TTAVTADAPGFNTLFTSAGLTWTICNIIGDGVTGTDKDGFYFPRMIKRMPSLENGLMV
                     INEDGSLTITYELRKGMKWHDGEPVTAHDAKFQWEVMNSGAPVTTNYFESSVSEVEVI
                     DDYTYSITLPEPLSNAELGSSVYAYYFGWFQLPEHVFRDSYEEAKHSGSWDSFVENAT
                     KNPIMTGPYKLKEYVEGQYVIMEAFDEYYMGRPNIDQLVMRIIPDMDVVFASTLNGEI
                     DFGRYTLTLKQSVQLENERADMFNVFYTPNIAYDNLNLNLRDPEDTSKPHPIFGDKRV
                     RQAVLYGLNREQISNVVYAGLAEVVDTWITDLHQMRDALKSPEVKHYEYNPAKAKALL
                     EEAGWKLNSKGIYEKDGTPLKFSLSLASGSGDYQMMAQIIQGMLKQIGMEVEIEVMPA
                     LVIWTEIFPYGDFDAHISGWGYGVSDEAANYWTTDQIPSEENYWGGMNFTGWANAEND
                     ELVNAAARELDPEKKLALYEKHFALWTEELPVLPLVVAPTPHFAKKYIKSFNSGYDNG
                     LGWIIQNWYIDEQ"
     gene            complement(9708..10559)
                     /locus_tag="PKL93_05445"
     CDS             complement(9708..10559)
                     /locus_tag="PKL93_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNQ70741.1"
                     /translation="MWAFLDGEFVQEKERLVQLENRGLTFADGLFEVIRTLEGRILFF
                     EDHYSRMKKSAEFFNIEMRFPSEEARRIAVELVKRNGIEDGELYIELTRGTDPHRDHR
                     YPPKETRSTFFMLALPLRKIDSTSWRDGVKLLTYPDLRHGLCEHKTINLLPNVLAKNF
                     AYIRGGYEALMFRESNGKKYATEGGSSNYFFVKDGIFYTPEVDNILPGITRGKVISLV
                     EDLGYEVVERRIALEEFFNAEEVFLASTVSRVMPVRAIDDVSFEVCGKHTAKVMRAYE
                     DLFFSAR"
     gene            10696..11157
                     /locus_tag="PKL93_05450"
     CDS             10696..11157
                     /locus_tag="PKL93_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70742.1"
                     /translation="MDNVLALFPGGSTGLFGSKVYDVVLIPEGIMLVQLTRELTNEAV
                     KKAKEASEERKEGFFKKMASVMSSGYSLLDRYNEKTKEEILNETPGNLLIPKESLRKI
                     KVSSNTNSSQGTSSDNTVIKIVWNEGKIKLTFRTDLNARKVKRVFEETFGL"
     gene            11211..13661
                     /locus_tag="PKL93_05455"
     CDS             11211..13661
                     /locus_tag="PKL93_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70743.1"
                     /translation="MRKSLPFFAIIALITATLMANCVLEFPNADIYYPEGYEEVAVNV
                     GNTFESIRSKAIELIGNDPGRINIVLADFGANTNGLAQPHNHKTIQIFVWPADTLLST
                     RMNESNHYRLLLMHEFTHIAHLTYTTGFPAFVSRVIFGTEMLSPQQLSPFVEGVTLFA
                     ESSNFFSEGRLNNPMWGREMTYQNLKSNSFPGLDYALSVSGEDYRGGALYYNYLATFY
                     DYLVKRFGIESVKQFHKEVSGRLPIIGPINASRIAFGETLDNLYLDWKEESELRTTKY
                     STLTEVRTVENGQIYDIAKTANGVIFSYSIFGEATSWNGSVERGIEEYVDGGFSRRLS
                     DFGALTLKWHEGNIYLMTSVTERNTTSREIWSHKLPLSVKLLDKGMITAFEIYNGRLI
                     KAVYDAKTEFSTIFFDEMQLIRIPWVVKDMLILEDGSLIMMLSRSGINGAIATFKEGE
                     FRVILDDPYLKGRGIDYYEGKVIFTAAYEEGFMDAFAVDLESLQISRLTHGANLEKAV
                     VLDSKVYGFGHSRREKGMSVFAFDFESTPYVVTAVESDDFEPLQVEYTVGDYLKKTIL
                     HFLSPVLRAPLVSYDGENISLGFLTVHLSHDSKHSMELKPLFTFGTSKLSFQGEYIGQ
                     ILEGVNVSLKVNLGTPNVPVATAGLSSELFQMPLNPSTRFRSYAHIGFDTSGGMTTSV
                     PLSLKGNLFSISLEPGIKLSDGEITPLLALDAGFSPTLRTSVNLGVGFFDDFYWYANG
                     AQLLYRIDWGWSPMFFLKEIGIGVEVSGTNTSLDHSALYMFVNVGSTLGLGDVFPKIG
                     IQHSDSGFGFYFELDAKP"
     gene            14094..15176
                     /locus_tag="PKL93_05460"
     CDS             14094..15176
                     /locus_tag="PKL93_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70744.1"
                     /translation="MKTMGYSAFLISDAGSVIIEESKHDGITLTFPSDPVFAKYRGRL
                     MAVIEVKMESSKFSLTKSKLDLSGVKLHGVKIVENKLFEDSVALWAFESRTLGKTLRI
                     VSDGEASKIPAKRDTNFVVPVIVNTIEFSSKEVHTGRLIFPLPDEWFVLSSVYFGQDG
                     AKIRRENAKIDLDSLSTGIVVEELRKHRVFTDNLWDLYQLYKSLENFETKNESLPEPM
                     GEEITVSDLDLEDSIPQAADLYLSKDRKTTDSLHREVVIQSSIEKLGTFSKKKAVVLR
                     IMNNSDLGTVKGIHISFRLIDPMGKELYVAEKELEGLSVGPGEAKKQVFTDFPEFADQ
                     QLERVVVNLMSISWSNGLKTLSASEE"
     gene            complement(15157..15996)
                     /gene="nadE"
                     /locus_tag="PKL93_05465"
     CDS             complement(15157..15996)
                     /gene="nadE"
                     /locus_tag="PKL93_05465"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNQ70745.1"
                     /translation="MDEIIGFIRDSIMKYGYKGAVIGISGGIDSAVVGKLAVEALGKE
                     KIFGLLMPERDSSRDTIRDSKLVAKFLGIDFKVKNISSALRAIGTYKLQPPAFLIPQT
                     IKEKYVKHKWEELSSDPFLDDLCNEGNEEFRRGLAYYRSKHRVRMISLYLEAEKRGYA
                     VLGTTNKTELLCGLYVKWGDDSSDIEPIRHLFKTEVIELAGQLQIPNKIIEKPPSPDL
                     IPGVTDENVFGVTIDDLDESLRRIEGGEVLDPHDTKSMRVLRILESARYRELRNLAIP
                     RKH"
     gene            16160..17842
                     /gene="ggt"
                     /locus_tag="PKL93_05470"
     CDS             16160..17842
                     /gene="ggt"
                     /locus_tag="PKL93_05470"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNQ70746.1"
                     /translation="MKKILFVLTIIVTAGTLMASNPINLYGRDAEGRNGVVAAAKPEA
                     SQVGVAILEMGGNAVDAAIATAFALGVLEPNASGIGGGGFMIIRLADMDDAVVIDFRE
                     TAPGATSPSFFNLDENNRPINYESTVGGKAIGVPGEVAGLLYALEHYGTLSREQVIKP
                     AIDWAKKGIPVTTNLFSIINDNYEKIMMMENGADIYLKYGGIPYEIGETIVLNDLANT
                     LELIAEKGKDAFYTGEIAEKIVAEVQKRGGVMTLEDLSNYEVKIRKPVCGSYRDYTIL
                     SVPPASSGGTHVIELLNIMENFDLESLGDNTAETLHIWSEAMKLVFADRAKYMADTDF
                     AEVPLEGLTSKDYAKLLADKIDMNRPIESVEAGDPWQFESGSTTHLSVMDKEGNMVAI
                     TKSINYFFGSGVVVPGTGILLNNHMDDFVKTPGSVNSVEPGKRPLSSMSPTLVLDPEG
                     RPYMTIGAPGATRIITTVAQTISNIIDHGMTVQQAILAPRVFRSQSGPFYIEGRISIN
                     AYNKLIQMGHEISIRNDYDPYFGGVHAVLFNRDIGILFGGADPRRDGQAVAF"
     gene            18018..18470
                     /gene="mscL"
                     /locus_tag="PKL93_05475"
     CDS             18018..18470
                     /gene="mscL"
                     /locus_tag="PKL93_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNQ70747.1"
                     /translation="MWKEFKKFISRGNVIDLAVGIIIGGAFQVIVKSLVEDIIMPIVS
                     LFLGGIDFSNIFISLSGGSYATLAEAKEAGAATLNIGLFINAVINFLILAFVIFMIVR
                     LINKIRERQKKEEAAAAPTTKICPFCHTSIPIDAIRCPNCTSELEKKA"
     gene            18640..>19475
                     /locus_tag="PKL93_05480"
     CDS             18640..>19475
                     /locus_tag="PKL93_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNQ70748.1"
                     /translation="MRLIKVLTLITALFVVLCFSLTGCIKPEVYYSLSGQVVPYFPET
                     SAHDSFQRTAAYTPIFSSKDIEQGFLSNQFIVLFDRGFDSTIISGVSGVEILDEFFSR
                     DGSVGYAVVKTNDPSSLSGIEGVKSVEPEKIFTLQSEESVPNDPLYSGQWNYPMIQMP
                     QAWTIAKGSSTVVVAVIDSGTRPDHPDLSGKLLSGYDVYNDDNDPIDEPPAGKVSHGT
                     HVTGTIASSTDNFVGVSGMIWSSYPVLLPVKVFEYDEYGRSVTTESILAKGIHYAVDH
                     GA"
     assembly_gap    19476..19575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19576..20042
                     /locus_tag="PKL93_05485"
     CDS             <19576..20042
                     /locus_tag="PKL93_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HNQ70749.1"
                     /translation="GYAGKDGSGEEIIRKILRETALDLGESGWDPYYGFGLVQAYDSL
                     SYDEERRPLLVQILSTSGEVIRETEVNSDGSFHFDGLTENYIKIRVWRDFNGNEEIDK
                     GDLIGYYGYQESDHYLVWNPSLDNAETLSYLSSGSYELTNPLHFFPIIEFSE"
     gene            complement(20093..20533)
                     /locus_tag="PKL93_05490"
     CDS             complement(20093..20533)
                     /locus_tag="PKL93_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNQ70750.1"
                     /translation="MPDANFRVRATSESAARVSVKARNFTMIVDEPPNLGGEDKGANP
                     VEYVLAALAGCLNVVGHLVADEMGFNIRRLEIDVYGPLNPARLYGKSYEDRAGYKEII
                     AEMKVDTDAEEETLEKWVKSVEDRCPVSDNLSNPTPVKVIAKEL"
     gene            complement(20910..22298)
                     /gene="cysS"
                     /locus_tag="PKL93_05495"
     CDS             complement(20910..22298)
                     /gene="cysS"
                     /locus_tag="PKL93_05495"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNQ70751.1"
                     /translation="MEIRLTNTMSRRKEIFKPLKIGEVGIYTCGLTVYNFAHIGNLRA
                     YVFADTLKRMFLFNGYKVRHVMNITDVGHLTGDEDEGEDKMEAGARREGKTVWEIVDF
                     YTKAFFKDLERLRIILPTVTCRATEHVQDMIDMIQKIEANGYTYIAGGNVYFDTSKVP
                     DYGKLARLQLDEEKIRSRVENDPFKKNPFDFVLWFTRYKYENHAMQWDSPWGRGFPGW
                     HIECSAMASKYLGERFDIHTGGIDHIPVHHTNEIAQSEAAFGHEWVNFWLHSEFLVIG
                     EGEKMSKSLGNFITLQTLIDRGYEPADYRYYLLGAHYKKQLTFTMEALDGAKSAMKKL
                     ITRIEELKELNESETRPNDDLMNEFHEAINDDLNTPKALAVLWKVVDSEDLRPGEKLS
                     LIDRFDEVLGLGLSEVESKTIPEEVIELANQRDAARKERNWEKADELRQLISEKGYEV
                     LDERDGYKIRRR"
     gene            complement(22329..23582)
                     /locus_tag="PKL93_05500"
     CDS             complement(22329..23582)
                     /locus_tag="PKL93_05500"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNQ70752.1"
                     /translation="MRKLAVVGAQWGDEGKGKVVNYFSENFEWIVRFSGGANAGHTIF
                     VEDKKYVNHLLPSINPRSCSKGFLGAGMVIDVEQLIDELETLEADFPGISSRFYLDPE
                     AFIVLPWHKEEDLFIEEMRKVPIGTTGKGIGPSYTDKVSREGLKLYVLFDESLLKERL
                     EALYHMKRMKYNRDFTLSFGEMTTYLNGLKTKLERLSVNFTSAVDMFRVFRSTSILFE
                     GAQGVLLDLDFGTYPFVTSGACMAHGVSSVGFSTFELDNVYGVLKAYTTRVGSGPFPT
                     EEFSEVGNLVRERGKEYGATTGRARRVGWLDLPALRYARIRSGLTGFVITKGDVLNGL
                     DEVKVCVAYDVEGAVKEMPSTSYDFFKARPIYETVSGWPSTDHINFLKYMTFIERETG
                     VEIDYISYGPRTEEMKTRNDLIMNI"
     gene            complement(23583..24884)
                     /gene="purB"
                     /locus_tag="PKL93_05505"
     CDS             complement(23583..24884)
                     /gene="purB"
                     /locus_tag="PKL93_05505"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNQ70753.1"
                     /translation="MIERYALSPLKDLWELRAQYERWLEVELAVVAAYEKTGLVPQGT
                     HDKIASSARVDLDSILKIESEVDHDVIAFIKSVTQNMGDEARYFHLGLTSSDVVDTAL
                     SLAITESGKLIIHALEKLSEALKERAVAYKDVVCMGRTHGVHAEPTSFGLKLLSFYVE
                     IKRAIDRLKKATENCAVGKLSGAVGNYANISLEIEKIALAELGLKPTVVSTQVIPRDV
                     HAEFFTVLAIAASSIERMATEFRHLQKTEVLEVQEPFREGQRGSSAMPHKKNPIICER
                     LTGMARIVRSYVLASLENISLWHERDISHSSVERIIFPDATMLLYYMAERSAYLVSNL
                     VVYPDRMRENFKASRNLVFSQRVMLSLVDRGMSREDAYQLVQRLSMNCWNNGLDFKEV
                     VLSNEEIAQYLDSEEAIRLFEPEYYLRNVGQIFERAFDGGN"
     gene            complement(25225..25614)
                     /locus_tag="PKL93_05510"
     CDS             complement(25225..25614)
                     /locus_tag="PKL93_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70754.1"
                     /translation="MFQRVPLPDTRLFILIPVPLVLLRRVIAIENIAVGVLFAGGISF
                     GLFSFGGLAFSILLPLGGVSSSLIFTLGGLAFSLYFAAGGLVASYDLALGGMAIARNY
                     ATEAFQWQMLPLGKTPEELHLLADRDW"
     gene            complement(25934..26377)
                     /locus_tag="PKL93_05515"
     CDS             complement(25934..26377)
                     /locus_tag="PKL93_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HNQ70755.1"
                     /translation="MKKIGIIICGRYQNCGGGKCLRAMREHVGGFALYPKDEELQLVG
                     YSYCGGCPGGNVEYVPQEMIKNGAEVIHLATGMVVGYPPCPRLSHFKEFIESYYEVPV
                     VIGTHPIPMKYLIAHQKLSFWEKTDMFSIAEHLMKEDPAIMKAYD"
     gene            26518..27849
                     /locus_tag="PKL93_05520"
     CDS             26518..27849
                     /locus_tag="PKL93_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ70756.1"
                     /translation="MKDFTKRMLSIAVPITLQNLISTGLNLVDNLMIGQLGTTAIASV
                     GLVNQVVFILNILTFGVSSGAGIFVAQFWGKRDEKSIEKTIGHMLYITIGASALFFVL
                     LFFLPERILMIFTTDVEVVKTGAEYARPVAFSTFLTSFSFIIAMALRTVEKARIPMYV
                     SLVALSINTVANYILIFGLGPIEPLGVFGASFATLLSRLVEFVIFVSIVYRKKTPIRL
                     SRSALKFEIPFFRRLMKYATPVIINEFLWSLGITMYSLVMARMGTEFIAARNISSTIE
                     NFGFVIFGGLSSATVVMVGAELGRNNFSQAKYNARKLLQLTVITAVATGFIIILLSRF
                     IVNLYNIDQSLKNTVLTVLIIIGLSQPIKMFNAVNIVGVLRSGGDSRAAMIIEIVSLW
                     GVGIPLVAITGLVLKWPLTLVYIAMMVEELFKSILGIRRTLTWKWLKNVVD"
     gene            complement(27924..29309)
                     /gene="pepV"
                     /locus_tag="PKL93_05525"
     CDS             complement(27924..29309)
                     /gene="pepV"
                     /locus_tag="PKL93_05525"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HNQ70757.1"
                     /translation="MDRKLDEMVIALKDELVSAVSDSVRVKSVQDTPVDGGPFGKGVK
                     ESLEKTVKLAKDLGFEARNADGYVGIVDYGSGETFGVLGHLDVVPEGEGWEVPPYSGL
                     VKDGEIWGRGTQDDKGPMIAALYALKAVKEYVKKPSKRIRLIFGTNEESGWECMKYYV
                     KHEEIPDMSVTPDADFPVIFAEKGIVNYGISAPAHNGGEGISVEVLSAGEAPNMVASS
                     ARAVLRGVDAESYEKIEAFSPKNDTKLKLTRSEDKVEILITGSSAHGSTPYKGQSAIA
                     AIVDLLADLRLGDSEIKNFLTAIKSKIGYEFYGESLGISGKDSMSGELTLNAGTLRLQ
                     AGVLKLVINIRYPVFFNESMIRSQIEEAFKGFRVETFHHQKPLYISPDSELVKMCREV
                     YKEVTGHDEEPIAIGGGTYARAVPNAVAFGALFPGNVEMAHQPNERVSIEDLLLVSRI
                     YAQLFLRFLQS"
     gene            complement(29322..30332)
                     /locus_tag="PKL93_05530"
     CDS             complement(29322..30332)
                     /locus_tag="PKL93_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNQ70758.1"
                     /translation="MYVVRTLIMAGGIGERLWPVSVKKRPKQFHKFGSSKTMIEETIE
                     RMEKVTDEIIIITTREQFPLMQYYLPLFPGENVIFEPMRRNTAPAIALGSTRFSPDDI
                     MVVVPADHVIRDEESFLKTLNKAIKYAESNDSLVTIGITPTHPYTGYGYIERGNAVDD
                     NEEVYSVKKFHEKPDYETAQHYVQSGRFLWNSGIFVWKKSVFDLELSKNFPDLFSAIS
                     EISKRPEDIEKIYERLPKISIDYGLMERSSKVATVPASFYWNDIGSWDAVYDLLIKDK
                     NGNAVEGEFSLKDVKNCLLINHTQKKLAVSGIEGFILVATSEGTLVCRRGESQDIKEI
                     IK"
     gene            30549..30941
                     /locus_tag="PKL93_05535"
     CDS             30549..30941
                     /locus_tag="PKL93_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNQ70759.1"
                     /translation="MTKVMIRLRMSLNDAHYGGNLVDGARILQMFGDVATELLIRNDG
                     DEGLFKAYDNIEFIAPVYAGDYIEATGEIISSGNTSRKMLFQAFKVVQTRPEINDSAA
                     ELLSEPVLVCRASGTCVVPKSRQRKRYE"
     gene            30934..31758
                     /locus_tag="PKL93_05540"
     CDS             30934..31758
                     /locus_tag="PKL93_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNQ70760.1"
                     /translation="MNKLIITAALTGAEVMKDQQPALPISPEEIADAAYDCFLSGASI
                     VHIHARDSSGKPTQNLEVYREIKERIAEKCDLIFQPSTGGAVWHKVRERAQPLELNPE
                     MATLSAGTCNFGEDVFFNSQDTMETFAQEMKARGIKPEIEVFERGMIENALKLLKKGL
                     IDSPIHFDFVLGVPGACPGNIEDLIHMVRAIPQGSTWTVAGIGRNELVLATAAILLGG
                     HVRVGFEDNIYYKKGELAISNAQLVERVVRISNELGRDVASPEEAREILGIRRKNQ"
     gene            31839..32786
                     /locus_tag="PKL93_05545"
     CDS             31839..32786
                     /locus_tag="PKL93_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HNQ70761.1"
                     /translation="MDLYDNEILVDVTTLNVDSASFTQIREACEDDVTKISSMIMDIV
                     RERGKLQNPVTGSGGMFIGKVSKIGKDLLRRDLEIGDTIASLVSLSLTPLNIERIVQV
                     HLEKDQIDIEGKAVLFESGIYARLPSDIPPKMALAVLDVAGAPAQVDKLVKKGMNVAI
                     LGGGGKSGLLCCYAAMKKCRDSGKVVVVEYSVENAERIRKLGLAHEVIIADATKPLEV
                     YEKITEFTQGDLCDVVINNVNVPGTEMGSILCAKDNGIVYFFSMATSFTRAALGAEGV
                     GKDITMMIGNGYTKGHAEFALGILRESSEIRRLFQKLYI"
     gene            32797..34065
                     /gene="ablA"
                     /locus_tag="PKL93_05550"
     CDS             32797..34065
                     /gene="ablA"
                     /locus_tag="PKL93_05550"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNQ70762.1"
                     /translation="MRDYREIPMWRDVTPAQWSDWRWQVANRVKTVDALRQIIDITEE
                     EAHGVAECLRTLRMSITPYYATLMDPNNQRCPIRRQAVPTDKELKIDKWDMIDPLHED
                     EDSPVPGLTHRYPDRVLFLITDQCSMYCRHCTRRRFAGQLDRARTRKEIDDAIEYIRE
                     TPEVRDVLLSGGDALLVGDDYLEYILSELREIPHVEIIRIGTRTPVVLPQRVTPELVK
                     MIRKYHPVWINTHFNHPLEITPDSTRACEMLADGGIPLGNQSVLLRGVNDSPYIMMEL
                     VHQLVKIRVRPYYIYQCDLSQGIGHFRTSIRKGIGIMESLIGNTSGLCVPTFVVDAPG
                     GGGKIRVMPQYNISESDRVVVLRNYEGVITTYHEPDDTSSDVDDRVYREKYEFTGVSD
                     LLYGNSMSFEPTTLQRRDRIKKWKEKKVRK"
     gene            34062..34472
                     /locus_tag="PKL93_05555"
     CDS             34062..34472
                     /locus_tag="PKL93_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNQ70763.1"
                     /translation="MKFEDFFVGQNFSTRRRITTEMVDSFANLTGDRNPVHTDEEFAR
                     KSRFGRRIAHGMLSVGIISAILGNEFPGPGSIYMKQEVRFLKPIFLEEEVLITIEILE
                     IKVEKKRLSVRTTVEKNNGELAVDGEALLLFEGE"
     gene            34469..35473
                     /locus_tag="PKL93_05560"
     CDS             34469..35473
                     /locus_tag="PKL93_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70764.1"
                     /translation="MKRRIRISDLAEGIEVISVIGLEKNTGKTVVLNQMLKEFREKGT
                     TAGVTSTGLEGEKRDQVYLNEKPEITLSRGTYFVTSERDFRHRSFSATVIDLRDYRSP
                     LGRTVFARAEADGKVIISGPSILEYLSEAVNTMRSYGCEKILIDGSTSRMSPGTPLVS
                     DGVILATGAALSMNLEELCRKTSFVVRLLSLPGTELELHKRIAPLGTGLWVEKDGVFE
                     RLSPSPLTAKSLKHELAGRTIYINGSLTSSVLLALAEKNTRIILKDFSRVFFDELTLA
                     KYERAGGHLEVLHPTHLIAVTVNPLAPNGFKIQSEKLIKSLESKIDVPVLDVLEESSN
                     "
     gene            35470..36846
                     /locus_tag="PKL93_05565"
     CDS             35470..36846
                     /locus_tag="PKL93_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNQ70765.1"
                     /translation="MKYLDCGFEYILSLIDPATPMGREELRAMTFLKRDEDIEAFHKR
                     QLEVNERKSQIEPLKSILARVRDIRGTISNLSPGKILDDIELFEIKSFAYWSERLRSE
                     IGECSSWMQLPDLSVVFSFLDPDDVGTESFYISDKLDNALAVVRKKLYRLQRNREEYD
                     ETSLNELIRLNFEIEEKVRADLSGKLITYREDLQSSATTIGKIDLIVALVEFNRKLGL
                     RKPEISSGEYEFEELVNPELAASLNKSGKNFQPFSASFDRRSVIIGGNMTGKSVLLRT
                     VALLQTMFQHGLFVPAKRAFIPVYDAIVYYSGDKQSFLDGLSSFAGEVDHLKKALEIV
                     HMDIRGLFLFDEIGRNTNPVEGPAFLMASAEYLSRNERCRTILTSHYEEALEIEGAKL
                     FIIKGLDHERMRNEIGREISYYVDHSLVEIESNAAFPREAVTIARLMGLDREFVELII
                     RKLKESCE"
     gene            36843..38405
                     /locus_tag="PKL93_05570"
     CDS             36843..38405
                     /locus_tag="PKL93_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HNQ70766.1"
                     /translation="MNGKLNLNMNKVKEARDYAQRIASEVHDFVEEHTTVSIERAICR
                     FFGIDGVDDYGIPIPNVVVDHLKSSNLINDGAAYVIGNAILETGLSPQKIAEEISFGR
                     LDLRSLSYRSQDEVFDRIDRLVNDAVGKVKLNREKRESLIGELGEGKKPYLYVIVATG
                     NIYEDVIQARAAARQGADIVAVIRSTGQSLLDYVPYGPTTEGFGGTYATQENFRIMRS
                     ALDDTGKEVGRYIRLCNYCSGLCMPEIAAMGAIERLDVMLNDALYGILFRDINMKRTL
                     IDQYFSRVINGFAGVIINTGEDNYLTTADAFEEAHTVLASQLINESLALMAGIPEEQM
                     GLGHAFEMDPSIENGFLYELAQAQMSREIFPKAPLKYMPPTKFMTGNIFMGYAQDTLF
                     NVISIMTNQGIQLLGMLTEAIHTPFMSDRYLAIENARYVFNNLRDLGDEITFKDNGII
                     QTRAATVLEEALQLLREIESIGLFETLERGTFANIKRSPHGGKGLDGVVRKGPKYRNP
                     FIEKMLGGRNHG"
     gene            38398..39174
                     /locus_tag="PKL93_05575"
     CDS             38398..39174
                     /locus_tag="PKL93_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HNQ70767.1"
                     /translation="MDNCSEKKNQFPSEIDLTRVIPYGDTLNDGKVQLSFTLPVPGGP
                     EADEAAKQMIHKMGLKNPQIVFSKEITIGFTFFVAYGEFSHAIDYTAIRVPKITLEKM
                     SMEEIDRFIEERIKRRIVVVGASTGTDAHTVGIDAIMNMKGFAGHYGLERYRNIDAYN
                     LGSQVQNEELVSKAIELDADAILVSQTVTAKNVHLRNMTSLVELLEAENIRKKIILIA
                     GGARITHELAKELGYDAGFGPGTFAEDVAAFIVHKLESMI"
     gene            39223..40125
                     /locus_tag="PKL93_05580"
     CDS             39223..40125
                     /locus_tag="PKL93_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNQ70768.1"
                     /translation="MKTLVVVNPNASHGEAGKDFEKSISSLLREEIADYDIHITTGPE
                     DALNFVTKNPNYDRIISVGGDGTLNEIVNGMIKGKSNASVGIVALGSGNDLARTINLK
                     HNYREMVRIAAGENVRKIDLLKVSYVDMNGVKASRYAVNVVGAGFDAAITVRMNKSRF
                     KTSGKMAYLLSFLMEFFTSKTYPLTFIVDGEETSDSYFFLTMANGNYFGGGMRIAPKA
                     LVDDGYFDVVGVSKMSKLRLLYHFPKIYRGEHLVVDTVSYRTAKNISLKSDRDVIIQM
                     DGEVVGSLPMEISICEKALKIASA"
     gene            complement(40117..40989)
                     /locus_tag="PKL93_05585"
     CDS             complement(40117..40989)
                     /locus_tag="PKL93_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70769.1"
                     /translation="MKIALGLIFVLVGVLLIFGLFTGDTLLNALVNLANFWPIILVLV
                     GMSILASIRGLRWIRYLNTILIVAFVLFVFFWPSPFLAGKKAVSDTVVLESSDSATSI
                     EIQLDLAMSNISLEPLRDEVASSTVAVVDYAVRGSGLKIEQDITQLSQRFIMKPESNV
                     SWLGTSTVVMKLNPAYDYVLKFNGAVMNCNLSLGELRVDELRINGAIVKAIVSMSAAG
                     FTNMKVNAAIINSEIHIPENIRAILKTSAAIKNVKTDLPYTGDGEYVFESVAFDFSSY
                     ITLESAIMNLKVLR"
     gene            complement(40994..41437)
                     /locus_tag="PKL93_05590"
     CDS             complement(40994..41437)
                     /locus_tag="PKL93_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNQ70770.1"
                     /translation="MSDTKRLYKSRKDKVIDGLAGGIAEYLGVDPVIVRLVFVALIFA
                     GGAGLIIYIIGMFIVPRAPINSENNVVIMDEEGKPIEEGKKEEFSDSKSKLIIAALLI
                     VFGIALLLGSFTPWNIFSGIFWKTVIGVVLIAGGGFVIYKSINRG"
     gene            41804..42817
                     /locus_tag="PKL93_05595"
     CDS             41804..42817
                     /locus_tag="PKL93_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ70771.1"
                     /translation="MANMKDVAKRAGVSVATVSNVMSGKKYVSPELKRAVLESIDALQ
                     YRPSKIARSLKIRKSFLVGLLVPDITNPYFAEIARGVESIALEHDYQMFLCNSDGRSY
                     REEETLISFLGHGVDGVINVAPRMDEELLADLSNGMPMVILDRHLKIENPLIDVVYTN
                     NFKGSAQLARHLLDKGHKRFACISGPKDVPSAMRRLQGFCSELEKDGIKGENLKIYFG
                     GFKYEDGFKIMKQFILKLKIRPTAVFAGNDMMAWGAIEAAKQNGLKIPEDIAIAGFDN
                     VLYSSLVIPSMTTVDQPKFEAGRTAMKLLLEKITKKQHGEYVDTRKIELDSHLIVRES
                     TQV"
     gene            42839..43282
                     /gene="rbsD"
                     /locus_tag="PKL93_05600"
     CDS             42839..43282
                     /gene="rbsD"
                     /locus_tag="PKL93_05600"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HNQ70772.1"
                     /translation="MKTKGILHNDLSREVARLGHGDMLVITDRGFPFPRHGNTVCIDV
                     SVGRNLPRFVDVVKVVLEELEIEKVIIADETRSVSPEIYSQLKEIVSKVKNKGNDIIE
                     ENIPHPEFKDLVLNGSLQGKEVKVFVKTGEFTPYANIILVSGVDF"
     gene            43350..44198
                     /locus_tag="PKL93_05605"
     CDS             43350..44198
                     /locus_tag="PKL93_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HNQ70773.1"
                     /translation="MKKNREVLSSVFKKEKPIIGMVHLRPLPGSPRYDPEKLGMKEIL
                     RIAVEEARIMEGCGVDGLQIENIWDFPYLKGERIGHETSAALAVATQSVREAVNIPVG
                     VNCHLNGGKAALAAAVAGGAKWIRIFEWVNAYVSHAGLTEGIGAELSRYRKLIGAEEI
                     KFMCDVNVKHGSHFIISDRSVEEQAHDAESEGAEILIVTGFETGQAPNAEKVKSFSEA
                     VSIPVMIGSGLTKENAGELLKFADGAIVGSYFKRDNNWKNPVDPNNVRSFMKTVESLR
                     EAGYDD"
     gene            44191..45135
                     /gene="rbsK"
                     /locus_tag="PKL93_05610"
     CDS             44191..45135
                     /gene="rbsK"
                     /locus_tag="PKL93_05610"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNQ70774.1"
                     /translation="MIDYAFNGEILCMGSMNMDLVMVMDRLPEPGETVLTDNFSTYPG
                     GKGGNQAVAAAISGARVQMFTKLGGDGFSEELIQMMKEKGVDTSRILRDPESTAGIAM
                     IRVDKNGQNSISFTPGSNARLSPEDVEENSDLFKRGRILLLTMEIAQETIFRAIKLAH
                     ENGMFTIVDPAPAPRNGFPDSIAPLINLIKPNESEARILTGIDVHDRESARIALQRLS
                     SMGFNLPIITLGEKGVVGVDGNDFMDLKPFDVHCVDTTAAGDVFSGALAAALSKGMHL
                     KESLKWANAAGALSTTISGAQTSIPAPKSVEKLLRRQV"
     gene            45139..46632
                     /locus_tag="PKL93_05615"
     CDS             45139..46632
                     /locus_tag="PKL93_05615"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNQ70775.1"
                     /translation="MPDIALKLEGIYKSFGGIHALKDVSFELSEGEVHALLGENGAGK
                     STLIKILTGVHKMDSGTIQVQGKTVTIEDPVDARHKGIGAIYQELSLVDSLTVAENIF
                     LGHEPITSFFGTLRKKELIRRAAEYLSSFGIEIDPSTFVGDLGLGQKRIVEIIKALSI
                     NANILLLDEPTTGMSRVEIDTLFEIMDDLKLKKVNMIYISHHLDEVFRVCDRATVLRD
                     GQNAGTFEIDKVDLKTLVKSMLGRTLKEEFPVREGRPSEETILEIEEFKTDKMLAPVS
                     FSLKKGEILGITGIIGSGKSELALGLFGADRAVKGKLSIAGKEMRLNSPDDAKRHGIS
                     FIPEDRKEQGLFLRLNVEHNICVANIDLAIKNGIISSKKKSEIAKETSEKLKIRPLDI
                     KMAVQNLSGGNQQKVVIGKWLCGEPDILILDEPTRGIDIGAKTEIYSLINYLADSGTG
                     ILILSSEFREMSAICDRILVIRKGTVVAELEGKDSSTEQLLTLALGG"
     gene            46638..47603
                     /locus_tag="PKL93_05620"
     CDS             46638..47603
                     /locus_tag="PKL93_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70776.1"
                     /translation="MENETEKNDRSAFVSKFLRLLKGELGLILILVALMVVFSLMSPY
                     FFTSRNLLNITRQVSITLIVAIGMTFVILTGEIDLSVGSSAALVGVATAAVLNATGSI
                     FLGLIAGLILGVGIGLANGVLTVYGRVQSFIVTLAMMGIARGAALVWTGGKPISRLPV
                     DFAIMGAGYLGPVPVSTIIAAGVFILAFFVLNRTKHGVYVKSIGANREAARLSAIPVR
                     KYRVLAFVISGLMSALGGILITSKLLSAQPTAAEGLEMNVIAAVILGGASLSGGVGTV
                     LGTLLGAMVIGVIDNGMNLVGVSSFFQQIVKGVIILVAVLAKRSD"
     gene            47633..48523
                     /locus_tag="PKL93_05625"
     CDS             47633..48523
                     /locus_tag="PKL93_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ70777.1"
                     /translation="MKRMFLVVLIIGLLALTGFAAKTIGFSVSTLANPFFVTMKEGGE
                     AKASELGLEMIVLDAQDKPETQFAQIQDLITRRVDVLIINPVDSDAIVSAVLEANAAG
                     IPVITVTRPSNGGVVEQHLDIDNKEAGMLAAVALAEALGGEGKVAILEGIPGAPSAVD
                     RQKGFMDELKKYPGIEVVASLTANYSREEGARVTEDILQGNPVLDGIYAHNDEMALGA
                     VRAVIAAGRLDEIKIVGIDATDDAIAAIKAGEMVATVQQQPYLQMQMAVIAAQRIING
                     ATVEPKVIIPLKLITIDVLE"
     gene            48835..49470
                     /locus_tag="PKL93_05630"
     CDS             48835..49470
                     /locus_tag="PKL93_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ70778.1"
                     /translation="MPKGTFFNLSETKRKRILEAAIDEFAENGYENSRVNEIVRRSSI
                     PKGSFYQYFENKSDLFRYVLDIIYKKKMEIMTGIEISHRELSVFETLRVMVEAGIKMA
                     EEDPRLLKISDSLVANKPLMDEVLIEYAPSSDDFIISLIRRGKNNGELADWVDPELSA
                     KLITAFMLSLSEMIRSSSNGKITDETREKYLSLINLLEYGMKRREDYDSGM"
     gene            49454..>49904
                     /locus_tag="PKL93_05635"
     CDS             49454..>49904
                     /locus_tag="PKL93_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70779.1"
                     /translation="MIQVCDLYYSYGKDEEFAVNGISFEVKEGEIFGFLGPNGAGKST
                     TQRILTGLLPVQKGKVRVMDVDVSATTPDFYNNIGVSFENPNLYLKLTGIENLEYFAN
                     LYDVPTEDPAHMLDMIGLKDVAKKRVGEYSKGMQQRLVFARSLINNPK"
BASE COUNT        14281 a        10924 c        11042 g        13457 t
ORIGIN      
        1 aattgaaaac ggagaaagaa ttgatgccct cgtcttttac aactctactt cgagaggatc
       61 ggcgtatgga gcacaaatgg ttcagaacgc tctggcttct tattcttcgt ttctcctgtc
      121 ggagaagctc ttggaacacg gtttaaccat cgaagacctc aaccctgtta atgtagagaa
      181 aatcgatgtt gctcccgagg agtctcaggg aacggaaatt ctcgcgacat tgatcccata
      241 tttgttactt atctatattt ttgccggagc aatgaacatt gggctagata caaccgctgg
      301 cgaaaaggaa agaggaagca tgcctgtgct cttggtgaat caggtttcta gatcatcgat
      361 cgcaacgggt aagattctct acgtaatgac catagcgatt ctgaacagca ttttcacttt
      421 cataggattg ataattgcct tcaaattagg aggcccggca tttggggcag agaacctaaa
      481 tttatcatct ctttcggtct cgactcttgt cggccttttc attacactgc ttaccatgtc
      541 gggacttgcc gccgctctga tcgttcttct gggttccatt gcaagaaacg taaaggaagg
      601 gagcggatac gtgatgccga tatatatcat ggctattgtc ctcggaatag caacgatgca
      661 gatggaatcg ccagataatc cgctgcttta tcttatccct ctggtaaatt caatcttcgt
      721 aatgaaggat attattacgg tcaatttcgt aactgctaga tttctgctta tgctaatcag
      781 taatctggca tacgtttcga ttttcattta ctttcttaca agggttttca acagtgaaag
      841 gataatggat agttctggat catgaaaggt tcaagataaa cccgattgta atgccattgg
      901 cgagccgagg ctctccaact ctttctccat tcaaggcaaa gtgagttatt attgagatag
      961 aacgttaata attgtaatgg gagaaatccg gttgaagaga atcttttcga ttggcgcttt
     1021 tatatttctg attattgtta cgctttttgg atctgagata cttgctgaca ggtaccttca
     1081 gcttttattg cgaggcaatt accatctcgc ttacgagatg caaagcgtta gagccagaac
     1141 tctctatacc gtcgataaca tggtgcagga gtttcagcag ttagaagagg agtatggcaa
     1201 ttatctgttc atcttctcca ccgaaacaag tgaattgaga ggctacacgg tatatatctt
     1261 ccacgctcag tttgagagag gattcattga cttcaacgtg gtggtagacg atcagggcaa
     1321 aatcgataca tttgtggtcc aaacgacgtc acagccgggt gcaatagctg aatatataga
     1381 tactagcaag ttcgacgaat ttgagatcac tataggtgat ggaagatacc gtctcccagc
     1441 agtaataacg gtccccaagg agatagacag gtacccgctt gtcattctga ttcacgattc
     1501 cggagctctt gacagagatt cgacagtagg tcccaacaaa cccttcagac agattgcctg
     1561 gggccttgcg acccagggcg tggccgttct tcgttatgac aagagaacat tcgtgttcgg
     1621 agagcgctta tcccagactt ctccaagtat cgagacggaa gtaatagaag atgtaatcaa
     1681 ggctataact gccgcttcaa gaattccttc ggtttcttcg atctttcttg ccggtcacgg
     1741 acttggagga agagttgctc ccaccatagc aatgagagat tcaagagtcg atggaataat
     1801 tctgatggct actccctcta gaagagagct ccaggtaata attgacaaac agaagtacat
     1861 ttcctcgctc aacaaggggg agattggaca acagactcag ctcctaacag attatctgca
     1921 gatcgctttg gaagggaagc tacctccagg agctcccgta cttgcagcga ccgccggtta
     1981 ctactacgaa ctcgacagga tgaacccaat agaaacaata agggagctgg aaatacccgt
     2041 gttgatagtg cagggtgacg ccgactttga gtcgaccgtc caggattaca taatgttcat
     2101 gaacgcactc tggacgaaaa tcaatgtcta cttccagttg ttgcccggcc tcgatcatta
     2161 cttcatgcct gtagagggtg agatatcaac gcctgacaac tactatgagt tccgacatgt
     2221 cgatggtcgt ctcatagata ccctattttc atggatctat attttcgact gagagacttg
     2281 ctcacagcgg ttcgcaattc aagacgcatc gagaacttct tcagaacaat ctattaatca
     2341 taaagaaagc acgaaacaaa gtgaccctca ctcacttcct tcaatgaagg gctttctttc
     2401 cagcatctat cgaatgccct cgggcatctc ttaacaaatc tgcacgtttc tggtggatcg
     2461 acaggtgagg gaatatcccc tttcactatc tctctatttc ggacacttct taagtgggga
     2521 ttcgggatag gaatcgagga taacaatccc ttagtgtaag ggtgcagagg atcgttaaaa
     2581 agagaatcgg tttcggccgt ttcaacaatc tttccaagat acatcacaga aatactgtca
     2641 cagatgtgtt ttacgactgc cagatcgtga gcaatgaaga gatatgtgag gccaaaatca
     2701 tgtttcagat cctcgagaag atttatcacc tgagcctgaa ccgagacatc gagagccgaa
     2761 accggctcat cacaaatgac cagactcggg ttaagaacta ttgaccttgc tattccaatt
     2821 ctctgcctct gaccaccaga gaactcgtga ggatagcgga acatgtactc cggtctaaga
     2881 ccgacctttt caagtatctc cgctatcatt gtcttgcgct cttctttgct cctaccaact
     2941 gaatggactc tcaaaccttc tgcaattata tcgaatacag tcannnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnacgggaa accattttct gagatcccta atctctacca
     3121 tcggagaggc tttcataata catcaccccc ttcgaaaagc caacatcgaa cagagtgctg
     3181 tctctcaatc tgaactactg gcggctgctg caggcacttt tcgaacgcta caggacatcg
     3241 gggcctgaat ctgcatcctt ccgggaaatt cgatggatgt ggcacgtagc cgtttattac
     3301 attgagcttc ttttgatcct tttttgcaac atctatctta ggtatagaac ttatgagacc
     3361 ttttgtgtag ggatgaactg gtttgtcaaa gatatcgaat actcctccgc tttcgacaat
     3421 ctggccggcg tacatcacaa caaccctgtc gcacatttca gcgattacgc ccaagtcgtg
     3481 agtaattatt atagttgcgg tgtcaaactt gctcttcaat tcgttcataa gttcaagcac
     3541 ctgggcctgt atcgtgacat ccagtgcagt agtcggctcg tcagctatca gtagctcagg
     3601 actgcaagca agagccatgg caatcattac cctttgcctc attcctcctg acagttgatg
     3661 aggaaactcc ttcactctct tctggggttc tggaattcct acaagtcgca gcatttcaat
     3721 gcttctcttc atcgcatcat cttcatcaag accttcgtga agtctgtaca cttctgagat
     3781 ttgccatccg attgtataaa gaggattcaa agaagtcatg ggctcttgaa ataccatgga
     3841 tattgacttt cccctgattt tctgtagctc ttctttactc agcgtagaga tgtcccttcc
     3901 cctgaaagag atcttagttt tgtcgcccag gaatgcgttc ttgggaagta gtttaataat
     3961 cgagaaagcc gtgatcgtct tcccgcaccc ggactctccc acgattccta gagtttcgcc
     4021 ttttgacaat ccaaaagaaa cgccatcgac cggcgttatc tttccgagct gtgtcctgaa
     4081 agagatttta aggttatcga cttctagtat tctctccatg aacttcaact ctcactcaca
     4141 aatttgggat ctaaagcgtc tctaagccca tcccccagga agtttacatt caacacggtt
     4201 atgaaaataa agaaccctgg aaagacggta agccaccagg gattagtacc agcagtggtt
     4261 cccaatatgt aattcattgc cctctgaagc atatttcccc atgaaggcat tggaggttga
     4321 atgccaagtc ccaggtaact cagcgatgat tcggcaagaa ttgcgtaaga catgttcaag
     4381 gtggccgaga cgataattat cggtataacg ttgggcagaa catgcctcaa tatgattctt
     4441 ccatttctca cacccatagc ccttgctgac attacgtatt cagtctctct tattgaaaga
     4501 accagtcctc taaccaatct tgaaactccc atccaaccaa acacggtaag tacaagaatg
     4561 atattcataa ttcccgaacc aaaaacgaga gtaagggtta tcaaaattgg gaacatcggt
     4621 atagaaagca ttacgtctac gaatctcatc aacaacctat caacgattcc accgtaaaaa
     4681 cccgaaaaca atcccacgag cgttcctatt agagtcgtaa tgactgctga agcaaaaccc
     4741 acaaataacg atatccttcc gccgtacata actcttacaa gtacatcatg tccaagttca
     4801 tcggtaccaa agggatgacc ctcagtcaac ggtggagaaa agagatttga aaaatccata
     4861 tcttcgtacc ctacctcaac gaagaggggg ccaagcagag tgaaaaacgc cacaccaatc
     4921 aacacgacgg caccaaaaag ggcaagtttg tggctcttga atttcgtcca cacgagttgt
     4981 ttgtagctgt ttatgtgaac aggtcgcgct ttctcctcta tctcatccag ttttctcttg
     5041 taaaaaaact ttgacataac taagctccct ttcttcccat tctgattctg ggatcgacca
     5101 ttacgtacag gaagtcggca acaagattcg aaatcaacgt tattattgcc agaaacatta
     5161 gacagcttat cgcaagatta tagtcgctgg aaataactgc attgtacata agccttccca
     5221 ttccgggcca tgaaaagaca gtttcaatga tcaaggctcc accgaagaaa tacggaatat
     5281 caagtgcaat tatagtgatt atcggtatca tcgcattttt cagcgcatgt ttaatgatta
     5341 cttttctgtc gggcactccc ttagcataag cagttcttac gtagtcttga tcgaggactt
     5401 cgagaagcga cgtcctgatg tatcttaccc atgatgccac ctgaaccaat cccagcacaa
     5461 aggcaggcag cacaaggtgt ctcactctgt ccgaaaagat attccaaaaa ccggttattc
     5521 cataggtcgc catacccgaa acggggaacc agcctaaatt cacactgaag atgataatgg
     5581 cgatcaaagc gaaccagaag cccggcaatg aaatgccgat aaaagcaaat atggtggttg
     5641 cgtagtcgaa ggtcgaatac tttttaacgg ctgaaataat gcctattgga agtgcaaata
     5701 caagcccgat tagccaggcg gtgatcgtaa gcactaacgt gttgggcaaa cgtgaagcaa
     5761 tcagatctat tacgggagac ttatacatgc ttgaataacc caggtcaccg gtcaaaacac
     5821 tcttcaacca ggtaaagtac ttaaccggaa gagggtcatc aagatgatag tactctctga
     5881 gttccttcat tctttcgggg tcattggtca ttgcacgagg gttttgcatc ctcatttgca
     5941 gcagtgggtc acctggcatt gagttcatta tgacgaatat tattattgag attgcgaaga
     6001 agattggaac aagttcaaga aacctcttta gaaaatacac cttcacgact tctagctttc
     6061 ccttctttga atatagtcgg cgacgttcaa acctgcaatc aactgaacac tgacaaggct
     6121 ctttatgggg actgcagtgt aattcagatt gtcttcaaga tactccattt ttctctcaaa
     6181 tgcctcatct gctatcttgg agatcgccgc tagctcctta gatccattgt cgttttctct
     6241 taataacctt cttagcattc cgagcatcaa tgattgatag aactggctga ctatcttatt
     6301 atcaaagacc tctgccatac ttgcctttct ctccagagat ggatcgtttt caatccagtt
     6361 cttctgcgct tgcagttgat cgggataggt ttcaagaaca ttcttgaaga ccctgtagaa
     6421 cggggaattt ctgtcaataa cgtatggttc aactgctttc atcacttcac tgacgaactc
     6481 aaaattctca acggaccttt gataattaaa tctctgcgct tccttcctcg taatatcggc
     6541 ttcagacatg tcttctaccc ttgaatcata gtaatatggc atctcacaaa ctaaggcaaa
     6601 cgtatcggct acggatgaag caaaatcatc agaactagtc ccggccttga tgacgtctgc
     6661 cgggtccttg tggggaccaa gattcttctt cagataatcg tactcttgag ccattccgaa
     6721 aagcttgaaa acggctggtt caagctgctc caaaaaaggt gcttcgggct ctccaagaga
     6781 aagcggtaaa tcaaacaaac cgggaagttt gctgaaagtc tcgtagagtt ccggaactgc
     6841 gtcactcaca taataataga cgcctccaaa accagaattg tgaagagaat acatgaactc
     6901 gggtttcttc tcctcgataa gtttcataag cacttgcgtc tctggtaaag gcgagtgaaa
     6961 gtgaagcgtt tcatagtcga ccgggaaggt ccattcaact tgctcatgac cgggcggtct
     7021 gtagaagttg gctgcgtatt tccttatgga tgaagaatcc ttgaaccaac cttcattcag
     7081 tttagtgcca tcaacatcta ttaccttaat caaatgccag gtgtagtcaa actgatcccg
     7141 aaaggtatcg tcgctggcaa gtttttcact taggaagtcc agcatcattg cgccaatcgg
     7201 ttcgttaggg tgcggacagc caaagagaag agcattcttc tttccgcttc caatttccag
     7261 tacgtagatt ggatttcctc cgcgtgattt tcccgcttcg taaactttga cttttccagg
     7321 atatctcttt gccagctcta gagatcgccg atccatctcc tcaacagtga aaaactcctt
     7381 ataatcggga acagaatcga taagctcgat gaacttcata tctaaacctc cgaaattatg
     7441 aaatttaaga tgggggagaa gattctattc tcccccaaag attcttattg ctcgtcaatg
     7501 taccagttct gaattatcca gcccaaaccg ttgtcgtagc cggaattgaa gctcttaatg
     7561 tatttctttg caaaatgggg tgttggcgca acaaccaggg gcagaacagg gagttcctcg
     7621 gtccaaagcg cgaagtgctt ttcatagagt gccagcttct tttccggatc gagctccctg
     7681 gcggctgcgt tgactagttc atcgttttcc gcatttgccc agccggtgaa gttcattcct
     7741 ccccaataat tctcttcaga gggtatctga tcagtggtcc aataattcgc cgcttcgtcg
     7801 ctgactccat acccccagcc tgagatatgt gcatcaaaat ccccataggg gaatatctcg
     7861 gtccagatta ccagcgctgg catcacctca atttctactt ccattccaat ttgcttcaac
     7921 attccctgaa ttatctgagc catcatctgg taatcgccgc tgccagaggc gagagaaagg
     7981 ctaaacttca gaggcgttcc gtccttttcg tatatgcctt tgctgttaag cttccagcct
     8041 gcctcttcga gaagagcttt ggctttcgcg gggttgtact cgtagtgctt aacctccggg
     8101 ctcttgagag catctctcat ctggtgcaga tcggttatcc aggtatctac gacttcggca
     8161 aggcctgcgt atactacatt gcttatctgt tctctgttta gaccatagag aacggcctgc
     8221 cttactctct tgtctccaaa gatcggatgt ggcttagatg tatcctctgg atctctcaaa
     8281 ttcagattca agttatcata agcaatgttt ggtgtgtaga aaacgttgaa catatctgct
     8341 cgttcattct caagctgcac tgattgtttt agagttagag tgtatcttcc gaaatcgatc
     8401 tctccattaa gtgtggatgc gaatacgaca tccatgtctg gaataatcct cataaccaac
     8461 tggtcgatgt tcggtctacc catgtaatac tcatcaaaag cttccattat cacgtactgg
     8521 ccttccacgt attcttttag cttgtacggt cctgtcataa tcggattctt tgtcgcattt
     8581 tccacgaaac tgtcccagct tccagaatgc ttcgcttctt cgtaactatc tctgaagacg
     8641 tgttcgggga gttggaacca tccaaagtag taagcgtaaa cagaagaacc cagctctgca
     8701 ttactcaaag gttctggaag agtgatagag tatgtgtaat cgtcgataac ttctacttcc
     8761 gaaactgaac tctcgaagta gttcgtcgta acaggagcac cactgttcat tacttcccac
     8821 tggaatttcg catcgtgagc agtaacgggt tctccatcat gccacttcat accctttctc
     8881 agttcataag ttatggtgag agaaccatct tcatttatta ccattagacc gttctcaaga
     8941 gacggcattc tctttatcat cctggggaaa tagaacccat ccttatctgt gccggtaact
     9001 ccatcgccga tgatattaca gattgtccat gtcaaacccg cagaagtaaa caacgtgttg
     9061 aaaccgggag catctgcagt aacagctgta gtggccgtac cacccttcgc gggtggattc
     9121 attgccatat aaatggatat tgccgcctct ttcctagtga ttggcgaact ggcctcgatc
     9181 acatggccgt acctgaagtc aagaagctgg cggttacttc tgtaagcaac ggtaagcgct
     9241 ccccaagctt cctccggagc ttcccactca tcgagagcag tcgtacaaat ctcgtcaaat
     9301 gcctctgctt cggactgcag tcccagaact tcaaccatag caactgcaaa atccgctctt
     9361 gaaacttcac ccttcccctt aatgatttct tccttaccag ggaaggcctt ttcaatagcc
     9421 gccttaaact ctgtgaaagt tagtggtgac tcgtcaactg aagtcaaaat ccctctagat
     9481 tcaattacag gtattgcttc ttctgcggaa atctgcgcca aagacagaac cgagaaaaaa
     9541 agaacgagca tacagacaaa gagtttcttc atcgcgtgac acctccgaga aagatgatgt
     9601 ggaacaagac agatttatgc aattatatac tggcttagct tacgaaaggc cagtaagcga
     9661 aatttacacc tattgtttac tttaatcgaa aattcgaacc gaatcgttca tctggcagag
     9721 aaaaagagat cctcataagc tctcatgacc ttagcagtat gcttgccgca tacttcgaag
     9781 gagacatcat ctatagccct aacgggcata acacgagaaa ctgtgctggc aaggaagact
     9841 tcctctgcat tgaagaactc ttccaaagcg atgcgccttt caacgacctc ataaccaaga
     9901 tcctcgacaa gagagatcac cttccctctt gtgatgccgg gaagaatatt gtcaacctcg
     9961 ggagtgtaga agatgccgtc tttcacaaag aagtaatttg agcttccacc ttcggtagcg
    10021 tacttttttc cgtttgactc tctgaacatc agagcttcat aacctcctct aatgtacgca
    10081 aaattcttgg caagaacatt tggtaataaa tttatcgttt tgtgttcaca gagtccgtgg
    10141 cgcaagtcgg gatatgtaag aagcttgact ccatctctcc agctcgtaga atcgatcttt
    10201 cttagaggaa gtgcaagcat gaaaaacgtg ctcctggttt ctttcggcgg gtatctgtga
    10261 tctctgtgag ggtcggtacc cctagtgagt tcgatatata attccccgtc ttctatgcca
    10321 tttcttttga ctaactcaac tgcaatcctt cttgcctctt cagaagggaa tcgcatttca
    10381 atattgaaga attcggcact ttttttcatc cttgaataat gatcctcaaa gaacagtatt
    10441 ctcccttcca gtgttctaat aacctcaaag agaccgtctg caaaagtcag tccgcggttc
    10501 tccagctgaa caagcctttc cttttcctga acaaattcac catctagaaa tgcccacata
    10561 gaaacctcct tgggtttttt gagtcactgc aacaatatta tgcatcaaac tggctatcaa
    10621 tttacggtaa gacagctcat cagtgttaga atataacgta actgtaagca gacttttcat
    10681 ttggagggtt tctttatgga caacgttttg gcgctttttc ccgggggctc tacaggactc
    10741 tttggaagta aggtgtacga tgttgtttta attccagagg gaataatgtt ggtgcagttg
    10801 accagagaac taacgaatga agccgttaaa aaagcaaagg aagcctctga agagcggaag
    10861 gaagggttct ttaagaagat ggcatctgtg atgtcgtcag gctattccct gcttgacaga
    10921 tacaacgaga agacaaaaga ggagatcttg aatgaaacac ctggtaatct cttaatccca
    10981 aaagaatcac tcagaaaaat caaggtctcc agtaatacga attcttctca gggaacttct
    11041 tccgataaca cagtaatcaa aatcgtatgg aatgaaggca agattaagct gactttcaga
    11101 acggatttga atgcaagaaa ggttaaacgc gtttttgagg agacgtttgg tttgtagctt
    11161 catcttgggg aatagtccct tcatcgatgg tctgaggcgg gaggtataaa atgaggaaat
    11221 ctttaccatt ctttgcaatt atcgctctta tcacggccac cttaatggct aattgtgttc
    11281 ttgaattccc aaatgctgac atttactatc ccgagggata tgaggaggtt gccgtcaatg
    11341 taggtaatac attcgaatcg ataagatcaa aagccattga actaatcggc aacgatcctg
    11401 gcaggataaa cattgttcta gccgacttcg gagcgaacac aaacggtctg gctcaacctc
    11461 acaatcacaa gactattcag atcttcgttt ggcctgccga tacattgctt tcgactagaa
    11521 tgaatgaatc gaaccattac aggctgcttc ttatgcatga gttcacacat attgctcacc
    11581 tgacctacac aacgggtttt ccagcctttg tctccagggt gatttttgga acggaaatgc
    11641 tttcacctca gcagctttca ccattcgtag aaggagtgac gctttttgca gaatcgtcta
    11701 acttcttctc cgaaggcaga ttgaacaatc caatgtgggg aagggaaatg acttatcaga
    11761 atttgaaatc aaactctttt cccggtcttg attatgcgct atccgtatct ggcgaagatt
    11821 accgaggagg ggcgctttat tacaattatt tggccacttt ctatgattat cttgtgaaaa
    11881 gatttggaat agaatccgtt aagcaatttc acaaagaagt ttccggaaga cttcccatta
    11941 tcggtcccat aaacgcctca aggattgcat ttggtgaaac tctcgacaat ctctatttag
    12001 actggaaaga agagtccgag ttgagaacaa caaagtattc cactcttaca gaagtcagga
    12061 cagttgagaa cgggcagatc tacgatattg caaagacggc aaatggtgta atcttctctt
    12121 attcgatttt tggggaagct acttcctgga acggttctgt ggagcgcggg atcgaagagt
    12181 acgtcgatgg gggcttttcc cgaagacttt ccgatttcgg agcactgact ttgaaatggc
    12241 acgaaggaaa catctacttg atgacgtctg ttactgaaag aaacacaacg tcaagagaga
    12301 tatggtctca caaactgcct ctatctgtta aacttctcga caaaggtatg attaccgcgt
    12361 ttgaaatcta caatggaaga ctgatcaaag cagtttatga tgcaaagaca gagttttcaa
    12421 cgatcttttt tgatgaaatg caacttatta gaatcccatg ggttgtcaaa gatatgctca
    12481 tccttgaaga tggaagtctg ataatgatgc tatccaggag cggtattaat ggagccatag
    12541 caactttcaa agaaggcgag ttcagagtaa ttctcgatga tccataccta aaaggaagag
    12601 ggattgacta ttatgaaggc aaagtgatct tcactgctgc ctatgaggaa gggttcatgg
    12661 atgccttcgc agtagattta gagtctttgc agatctctag gcttactcac ggtgcaaatc
    12721 ttgaaaaagc agtcgttcta gactccaaag tctacgggtt cggacactct cgtagagaaa
    12781 agggtatgtc tgtttttgct ttcgattttg agagtacccc atatgtcgtg acagcagtcg
    12841 agtctgatga ctttgagcct ttacaagtag agtacacggt gggggactac ctgaagaaga
    12901 ccatccttca ttttttgagt cccgtcctaa gagcgccgct tgtatcatat gatggcgaaa
    12961 acatttccct aggatttctt accgttcacc tttcacatga ttcgaaacac tctatggagc
    13021 tgaagcctct attcactttt ggtaccagca agctttcctt ccagggggag tatattgggc
    13081 agatcttgga aggagtaaat gtctctctga aagtaaactt aggcactccc aacgttcctg
    13141 tggcaactgc agggctatct tctgagctct tccagatgcc tttgaacccc tccacgagat
    13201 ttagatcata cgcgcacatt ggatttgaca catcaggggg aatgacaact tcagttcctt
    13261 tgagtttgaa aggaaatctc ttctcgatct ctctagaacc cggcataaaa ctctctgatg
    13321 gggagatcac tccactgctc gctcttgatg ccggtttttc cccaactttg cgaacttctg
    13381 tcaatcttgg tgtaggtttc ttcgacgact tctactggta tgcaaacggc gctcagcttc
    13441 tgtatcgaat cgactgggga tggagtccca tgttcttcct gaaagagatt gggatcgggg
    13501 tcgaagttag tggaaccaat accagccttg atcactctgc gctgtacatg tttgtgaacg
    13561 taggttctac cctgggcttg ggagatgttt tcccgaagat aggaattcag cacagcgact
    13621 ctggttttgg gttctatttc gagttggatg ctaagccttg atataagacc tggcttttta
    13681 taacgatcca gatgaaaaac tacgggagaa agggggaacc cctttctttt tttacctcga
    13741 cgattcaaaa gctaaatctg gaggttaaga ggaaggctct aaattcatca aaacacttac
    13801 agataaaaga acttcgggag ccgaaaagag cttattcttt tcaggtccca ataagagaaa
    13861 tccgtcgaga tcatttcatg tgattttctc attatcaatt ggtttcgatg aaatctgatt
    13921 taggttcgtt tccagtgata tttccgagtt ttcttctact tttgaccacg gtggaagggc
    13981 tttcactcga gaaaactcag tagctgccct ttagttaact ttgcttaaga catgacgaat
    14041 ggataaggta taatgctttt atcggagata gcaaaaagaa agggggaatc aacatgaaga
    14101 caatggggta ctcggctttt cttataagcg atgccggttc agtgattatt gaggaatcaa
    14161 aacatgacgg aataacgctc acatttcctt ccgatccagt atttgcgaag tataggggcc
    14221 gtctaatggc tgttattgaa gtcaaaatgg aatcatcgaa gttctctctc acaaagagca
    14281 agcttgacct tagcggggtt aagcttcatg gcgtcaagat agttgaaaac aagttatttg
    14341 aagattcggt tgctctttgg gctttcgagt cgagaacgct cggcaagact ctgagaattg
    14401 tatcagatgg agaagcgtca aagatacctg caaaaaggga tacgaatttt gttgtcccag
    14461 ttattgtgaa tacaatagag ttctcctcga aggaagtgca tactgggaga ttgatctttc
    14521 cgctgccaga tgaatggttt gttctttcca gtgtatattt tggccaggat ggcgcgaaaa
    14581 tccgcaggga aaacgcaaag atcgacttag attctcttag tacaggaata gttgtggaag
    14641 aacttagaaa acacagggtc ttcaccgaca atctttggga cctgtatcaa ctgtacaaat
    14701 cattggaaaa ctttgaaacg aaaaacgagt ctcttcccga accaatggga gaagaaatca
    14761 ctgtttcgga tctagacctt gaagactcta tacctcaggc tgccgacctt tacctttcaa
    14821 aggacagaaa gacaaccgac tctcttcatc gcgaagtagt tattcaatcg agcatagaaa
    14881 agctgggaac tttcagcaaa aagaaagcgg ttgtcctacg gattatgaac aattccgacc
    14941 tcggaacggt caagggaatt catatatcat tcaggctcat agatcctatg ggtaaagagc
    15001 tttacgttgc agaaaaagaa cttgagggat tgagtgtcgg accgggagag gcaaaaaaac
    15061 aagtgttcac agactttccg gaatttgccg accagcaact ggaacgagta gttgtgaatc
    15121 tcatgagtat ttcgtggagc aacggtctaa agacgcttag tgcttccgag gaatagctag
    15181 atttcttagt tctcgatacc tggctgattc aagtattctc aggaccctca tggatttcgt
    15241 gtcatggggg tctaaaactt ctcctccttc aatacgccga agagattcgt ccagatcgtc
    15301 aatagtcact ccaaagacat tttcgtcagt aacaccaggt atgagatctg gacttggagg
    15361 tttttctatt attttgtttg gaatttgcaa ttgccctgct agttcgatta cttcggtttt
    15421 aaacaaatgc cttattggct caatatcgct tgaatcgtct ccccatttca cataaagccc
    15481 acataacagc tcggtcttgt tggtggttcc cagcaccgca tatcctcgtt tttcagcttc
    15541 aagatacaga gagatcatcc ttactctgtg cttcgatcta taataggcaa gtccccttct
    15601 gaattcttcg tttccttcat tacaaagatc gtcaagaaat ggatcagaag acagctcctc
    15661 ccacttatgt ttaacatact tctccttaat tgtctgaggg attaagaatg cgggaggctg
    15721 aagcttatag gtaccaattg ctctaagagc agagcttatg tttttgactt tgaagtctat
    15781 cccaagaaac ttcgcgacga gcttcgaatc cctaatcgta tctcttgaag agtcgcgctc
    15841 tggcatgagt agtccgaata tcttctcctt tccaagagct tcgacggcga gttttccaac
    15901 aacggcagaa tcgattcctc cgctaatacc tatcacagct cccttataac catatttcat
    15961 aatagaatct cttatgaaac caataatctc atccataatg ttcacctagg aaattatact
    16021 agatgaatga tcttttccct tgaaaagtca tagccatttc gcagtaagta atcagactct
    16081 ttgacaggga atgttacgtt ggatgaaaat gctaaacttg taattatgaa gctcttaagc
    16141 tcaaattagg aggacgataa tgaagaaaat tctgtttgta cttaccatca tagtaactgc
    16201 cggaactctc atggcttcga atccaatcaa cctttatgga agagatgcag agggcagaaa
    16261 cggagttgtg gcggcagcaa agcctgaggc ttcacaggtg ggcgttgcaa ttctagaaat
    16321 gggtgggaat gcggttgatg cagcgatcgc cactgcattt gcgctgggtg tacttgaacc
    16381 taacgcatca ggaatagggg gcggcggatt catgatcatc aggttggctg acatggatga
    16441 tgctgtcgtc atagacttca gagaaactgc accaggagcg acgagtcctt cattcttcaa
    16501 tctcgacgag aacaatcgtc ccatcaacta tgaaagtact gttggtggaa aagccatagg
    16561 agtacccggt gaagtagccg ggcttctata tgcgcttgag cactatggaa cactctcccg
    16621 cgagcaagtt ataaaaccgg ctatagactg ggcgaagaag ggaattccgg tgacaaccaa
    16681 cttgttctcg attatcaatg acaactacga aaagataatg atgatggaaa acggtgccga
    16741 tatctacctt aagtatggcg gcatacccta tgaaataggt gagactattg ttctgaatga
    16801 tttggccaac actctcgagc taatcgcaga gaaaggaaaa gatgcattct acaccggcga
    16861 aattgccgaa aagatcgtcg ccgaggttca aaaacgtggc ggagttatga ctctagaaga
    16921 cctttcaaat tacgaagtga agatccggaa accagtttgc ggaagttatc gcgactatac
    16981 aattctttcg gtgcctcccg caagttcggg tggaactcac gttattgaac tgctaaacat
    17041 tatggagaac ttcgatcttg aatcacttgg agacaatacc gctgagactc tgcacatttg
    17101 gtctgaagca atgaagctgg tatttgccga cagggccaaa tacatggccg ataccgactt
    17161 tgcagaagta ccccttgagg gcctaacttc aaaagattat gcgaaactgc tggccgacaa
    17221 gattgatatg aacagaccaa tcgaatcggt ggaggccgga gacccctggc agtttgagag
    17281 cggcagcacc acgcatcttt ccgtaatgga caaagaagga aacatggttg caataaccaa
    17341 atcgattaac tacttcttcg ggtcaggtgt cgttgttcct ggaaccggca ttctgctaaa
    17401 caatcacatg gacgatttcg ttaaaacccc aggttccgtc aattcagttg aaccaggcaa
    17461 acgacctctc agcagcatga gccccactct ggttttggac cctgagggaa gaccatatat
    17521 gacgattggc gctccgggtg ccacaagaat tattaccact gtggcccaga caattagcaa
    17581 tatcattgat cacgggatga ctgttcaaca agcgattctg gcccctagag tcttcagatc
    17641 tcagtctggg ccattttaca tagaaggccg catctcaata aatgcttaca acaaactgat
    17701 ccaaatgggt catgagatat caataaggaa tgattatgat ccttattttg gaggggttca
    17761 tgcagtgctt ttcaacagag atataggcat tctttttggc ggagccgatc ccagaagaga
    17821 tggacaggct gttgcgttct gatgtagcac agaagatcac ctcttcgaaa acaaagcaac
    17881 gatcttttct tttttaagga tgaaactcag taatcaaatc ggtaatcgct ctttgattgt
    17941 gtttcaaatc cttgattata gaacgataca tgatcataat ctcttcataa cgtgcgtatt
    18001 gtagaggggt gagcggtgtg tggaaggaat tcaagaaatt cattagtcgt ggtaatgtga
    18061 ttgatcttgc ggtcggaatc attatcggcg gtgcgtttca ggtcattgtc aaatcgctgg
    18121 tcgaagacat tatcatgcct attgtcagcc tatttcttgg tgggattgac ttttcaaaca
    18181 tattcatcag tctctccgga ggaagctacg caactctcgc agaagcaaaa gaagcaggtg
    18241 ccgcaactct gaacatcggc cttttcatca acgcagtcat aaatttcctg atccttgcgt
    18301 ttgtcatctt tatgattgtg aggctcatta acaagatacg ggaaaggcag aagaaggaag
    18361 aggcagcggc agcgccaacg acgaagatct gtcctttctg tcacacttcg attccgatcg
    18421 atgcaataag gtgtcccaac tgtacgtctg aacttgaaaa gaaggcttga gacccttttc
    18481 gaagagagcg acctacgggt cgctcttgtt tttggatatt tccattcgtc gctcttgtcc
    18541 gtaccgattt acagaccgga cccatttcat caaaattgaa taacacgaaa actcttgcga
    18601 tgatggtaca atcattccat ctagatccgg aggttcaaga tgcggcttat taaagtctta
    18661 acgcttataa cagctctttt tgttgttctt tgtttctctc tcactggatg cattaaaccc
    18721 gaggtttact actctctttc aggccaggtc gttccttact ttccagaaac ctcagctcac
    18781 gatagttttc aaagaactgc agcatacact cccatatttt ccagtaaaga catagagcag
    18841 ggtttcttga gtaatcaatt tattgtcctc ttcgatagag ggtttgattc aacaataata
    18901 tcgggcgttt ctggagtaga gatcctcgat gagttctttt caagagacgg ttctgttggt
    18961 tatgccgttg tgaagacaaa cgatccatct tcgctgtctg gaattgaagg agtcaagtca
    19021 gtcgagcctg agaagatctt cactcttcaa tccgaggaga gtgtccccaa cgacccgctg
    19081 tattccggtc aatggaacta tccgatgatt cagatgcctc aggcctggac tattgctaag
    19141 ggttcttcca ctgtcgttgt cgcagtaata gactccggaa caagacctga ccacccagat
    19201 ctatcaggca aactgctttc tggatacgac gtctacaatg atgacaatga cccaatagac
    19261 gaaccccccg caggcaaggt ttcacacgga actcatgtga caggtactat agcttcatca
    19321 accgacaatt tcgtgggtgt ttctggaatg atctggagca gttatccagt tctccttcca
    19381 gtaaaagtct tcgagtacga cgaatacgga agaagcgtca caacggagag cattcttgcg
    19441 aagggaattc attatgcagt cgatcatgga gccaannnnn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnaagga tacgccggca aggacggctc aggtgaggag ataataagga
    19621 agatcctgag agaaacggct cttgatctgg gagaaagtgg ctgggatccc tactatggct
    19681 tcggactagt tcaggcatac gattctctta gctacgacga agaacgtagg ccactattgg
    19741 ttcaaatctt atctacctcg ggcgaagtca ttagggagac tgaagtcaat tcagacggtt
    19801 cctttcactt cgatgggctc acggaaaact acataaagat aagggtatgg agagatttca
    19861 acggaaacga ggagatagac aagggagacc ttatcggcta ttacgggtat caggaatccg
    19921 accattatct cgtctggaac ccctctcttg ataacgcgga aactttatct tatctcagca
    19981 gcggtagtta cgaactcact aatcccttac actttttccc aataatagaa ttttcggaat
    20041 aagactaatt aaaagggctc ccttcaggga gccctttttt aaactaagtc gcttagagtt
    20101 ccttagcaat gaccttaact ggtgttggat tactaagatt gtcggaaacc ggacatcgat
    20161 cctctacaga ctttacccac ttttccaggg tttcttcttc ggcatccgta tcgactttca
    20221 tttcggcaat aatttcctta taaccggccc tgtcttcgta ggacttccca taaagccttg
    20281 ctggattcaa cggaccatag acgtctatct ccaatcttct tatattgaaa cccatctcat
    20341 ctgcaacgag atgccctact acatttagac aaccggccaa tgcggcgaga acgtattcca
    20401 ctggattggc acctttatct tctccaccaa gattgggtgg ctcgtcaacg atcatagtga
    20461 agtttcttgc ctttacggat actctggctg cactttctga agttgctctt actctgaaat
    20521 ttgcatctgg catactattt cctccttttt tgttacttct ttcacaaata gtataatagt
    20581 catcgggcat gagagagtta agccgagata aattatccgt aaataagaca actatcactc
    20641 gttgtttggt tagacagcct tgcactctgc ttcttttcta agatgccgtt ccttactctt
    20701 taggaagcgg ttcacgcctt agcttgcagc tagttttgca gcacaaaaaa ccaatgaagg
    20761 aaagaccttc gattatttgg aaaagacctc ttctgctcac atcaaagcag tgaggttatt
    20821 cttactatat agggagctaa tcgcgaaatc ccatttacag gaaatatcca gataattcaa
    20881 cgctcgtagc gggagaaagt agtctttgat taccttcttc ttatcttata cccatctctc
    20941 tcatcgagta cttcatatcc cttttctgag atcaactgtc taagctcatc ggccttctcc
    21001 caattccgtt cctttcgagc agcatctctc tgattggcca attcgataac ttcttctgga
    21061 atggtcttgc tttcaacctc agagaggcca agaccaagaa cttcgtcaaa tctgtcgatt
    21121 agggaaagct tttcaccagg tcgaagatct tcgctgtcta ctactttcca aagaacggcg
    21181 agggcttttg gtgtattcag atcatcattt atagcctcat gaaattcatt cattagatca
    21241 tcattgggcc tggtttctga ttcatttaat tcctttagct cctcaattct agtgatgagt
    21301 ttcttcatgg cgctcttcgc gccatcgaga gcttccattg taaaggtcag ctgcttcttg
    21361 taatgggcgc cgagaagata gtagcgataa tctgcaggct cgtaccccct gtcaataaga
    21421 gtctgaagag taatgaagtt tccaagcgat ttagacattt tttccccttc cccaatgacg
    21481 agaaactccg aatgcaacca gaagttcacc cattcatgtc cgaaagccgc ctcgctctga
    21541 gcgatttcat tcgtatgatg aaccggaatg tgatctatac cgcctgtgtg tatgtcaaat
    21601 ctctctccaa gatactttga agccatcgcg gaacattcaa tgtgccatcc gggaaagccc
    21661 ctaccccatg gtgaatccca ctgcattgcg tggttttcat atttatatcg agtgaaccac
    21721 aaaacgaaat caaaggggtt tttcttgaag gggtcgttct caacccttga acgaattttc
    21781 tcttcatcaa gttggagcct tgccagtttt ccatagtcgg gaacctttga agtatcgaag
    21841 tagacattac cgccggctat gtaggtgtag ccgttggctt caattttttg gatcatgtct
    21901 atcatatctt gcacgtgctc ggtcgccctg cacgtaacag ttggaagaat gatcctcaat
    21961 ctttctagat ctttgaagaa agccttcgta tagaaatcaa cgatttccca cactgtcttt
    22021 ccctcacgtc tcgcaccggc ttccatcttg tcttcgcctt cgtcttcgtc tcccgtaaga
    22081 tggccaacat cagttatgtt cattacgtgt cgtactttat agccattgaa aagaaacatc
    22141 cttttgaggg tatcggcaaa gacgtaagct ctaagattgc caatatgggc aaaattatat
    22201 actgtcagtc cacacgtata tattcccacc tcgcctatct tcaaaggctt gaagatctct
    22261 ttccttcttg acatggtgtt cgtcagtctt atttccatac tctgcctcac ctcattccca
    22321 gttaccttct aaatattcat tataaggtcg tttctagtct tcatttcctc tgttctagga
    22381 ccgtacgata tgtagtctat ctccacaccg gtttctcttt caatgaacgt catgtacttc
    22441 agaaagttaa tatgatccgt ggaaggccag ccgctaacag tttcataaat cggtcttgcc
    22501 ttgaagaaat catatgaagt tgaaggcatt tccttaaccg caccctcaac atcataagcc
    22561 acacaaacct tgacttcatc caacccattt aacacatcgc ccttagtgat gacgaaacct
    22621 gtcaggcctg atcttatcct tgcgtacctg agtgccggaa ggtcaagcca gcccactctt
    22681 cgagccctcc ccgttgttgc accgtattcc ttgcctcttt cgcgaacgag gttccccact
    22741 tcagagaact cttcggtcgg gaaggggcca gaacctactc gagtcgtgta ggctttcaga
    22801 accccataca cgttgtcaag ttcgaaggtc gaaaaaccca ccgatgatac accgtgagcc
    22861 atacatgctc cagaagtgac aaacgggtat gtcccgaaat caagatcgag aaggacacct
    22921 tgagctccct caaagagtat ggaagtactc ctgaagaccc tgaacatgtc taccgcgctg
    22981 gtgaaattga cgctgagcct ttcgagctta gtcttgagcc cattcaaata ggtcgtcatt
    23041 tcgccaaatg aaagtgtgaa atccctgttg tatttcattc ttttcatgtg atagagagct
    23101 tcaagtcttt ctttcaacag tgattcatcg aagagaacgt agagcttcaa cccttcccgg
    23161 gagaccttgt cggtgtagga aggacctatt ccttttcctg tagtccctat tggcaccttt
    23221 cgcatctctt ctatgaacaa gtcctcttct ttgtgccatg gaagaactat aaaggcttca
    23281 ggatcaagat agaatctact tgaaatacct ggaaagtccg cttccagtgt ttccagttca
    23341 tcgatcagtt gttctacatc tatcaccatc ccggcaccaa gaaaaccctt tgaacaacta
    23401 cgaggattga tcgaaggcaa aagatggttc acgtatttct tgtcctcaac gaaaattgta
    23461 tgaccggcgt ttgcaccacc agaaaatctg actatccact cgaaattttc ggaaaagtaa
    23521 ttgaccactt tccctttccc ttcatcgccc cattgggcac cgacaacggc aagtttcctc
    23581 acttagttac ctccatcaaa agctctttca aatatttggc cgacgtttct caaataatac
    23641 tcaggttcaa aaagccgtat tgcttcttct gaatcaagat actgggctat ttcttcgttt
    23701 gataagacga cctccttgaa atccagccca ttattccaac aattcataga gagtctttga
    23761 accaattgat aggcgtcttc cctggacata cccctgtcaa caagtgacaa cataaccctc
    23821 tgtgagaaga caagatttct agaggccttg aagttctccc tcatcctgtc aggatataca
    23881 acaagattgc taaccaaata ggctgacctt tctgccatgt aatagagaag catagtggcg
    23941 tccggaaaga ttatcctctc tacactggaa tgcgagatgt ctctttcatg ccagagagag
    24001 atattctcta atgacgctag cacataactc ctgactattc ttgccatacc tgtaagtctt
    24061 tcacaaataa tgggattctt cttgtgaggc attgcggaag aacctctttg accctctctg
    24121 aaaggctcct gaacctccag gacctcggtc ttctgaagat gcctgaattc ggttgccatt
    24181 ctctctatcg atgaggcggc tattgccaac accgtaaaaa attctgcgtg aacatctctc
    24241 ggtattacct gagtcgaaac tacagtgggt ttcaagccaa gttccgcaag agcaattttc
    24301 tcaatctcga gtgaaatgtt ggcgtagttc ccaacggcac cacttagttt cccaaccgcg
    24361 caattctccg tggccttctt cagacgatct attgccctct ttatctccac gtaaaaagat
    24421 agaagcttta aaccaaacga agtcggctct gcatgaacgc cgtgtgtcct tcccatgcaa
    24481 acgacatctt tataagcgac cgctctttct ttcaacgcct ctgagagctt ttccaaagca
    24541 tggattatga gctttcccga ctccgtaatt gcaagtgaaa gcgctgtgtc tacaacatcg
    24601 gaagaagtaa gtccgagatg gaagtatctc gcctcgtccc ccatattttg tgtcactgat
    24661 ttgataaagg ctattacatc atgatcaact tcgctttcaa tcttcagtat tgagtctaga
    24721 tccactcttg ctgacgatgc aatcttatca tgcgtaccct gaggaactaa ccctgtcttc
    24781 tcgtacgccg caactacagc cagctccact tcaagccatc tttcgtattg ggctctaagc
    24841 tcccagaggt ctttcagggg tgaaagcgca tacctttcta tcatgaccca cctccatgac
    24901 caacaaataa tagtttacat caaagctaac atcaatgtct ttcggaaacg tcatccattt
    24961 caaaagatcc ttcggcccga ccgttgaacc aagaaagcga aaactcgcaa cacatagcga
    25021 cgcttttgca catcacaaga agtatgcgta attagctgaa gagactattc acacgatcaa
    25081 atcgaagact gaaaattctc caggagaatg aaatgtcaat agattgttct gattatccat
    25141 tcaaagagag tgcttatctc tacctttcta gatgtcacaa agtctattgt ctgatcggct
    25201 atcttcgtta atctaagcag ataatcacca gtccctgtct gccaatagat ggagctcctc
    25261 tggcgtcttc cccagtggca acatttgcca ttgaaaagcc tcagtcgcgt agtttctggc
    25321 gatagccatt ccaccgaggg caagatcata ggaagcaaca agacctccag ccgcaaaata
    25381 caaagagaag gcaagaccac caagtgtaaa tatcaggctt gatgatactc ctcctagggg
    25441 aagcaaaatg gaaaacgcta atccaccaaa gctgaataga ccgaatgaga taccgccagc
    25501 gaagagtaca cctactgcaa tgttttcaat tgcgatgacc ctccttagca ataccaaagg
    25561 aactggtatt aatatgaaca atcgagtgtc cggcagtggc actctttgaa acacactcat
    25621 atccatagag acttcgctta cgaccagtaa ccgtagctct tgagtatgcc tttggataaa
    25681 gcggcttcct ttcttccaaa aacctccttg cacactcatt gggggaggtt tacaccttga
    25741 aagaattatc catgccactg tcgaaaactc tgttttggat atcgtaacac atctctcttc
    25801 tcaggtcttt tttggagatt ctaagaatag aattcgagct caaagagatc cgcaagtcaa
    25861 gtactgagaa gccctcttct cgttgatcat tccactcacc ggtgtcccat actgcagaac
    25921 ccagctctca cactcaatca tatgctttca ttatcgccgg gtcttctttc atcaagtgtt
    25981 ctgcgataga aaacatatcg gttttttccc agaatgaaag cttctgatga gcaatgagat
    26041 atttcatggg aattggatga gtgccgatta caaccggtac ttcataatag ctttcgatga
    26101 actccttgaa atgactcagt ctcggacatg gtggatatcc cactaccatt cccgtcgcca
    26161 ggtgtatgac ttctgcaccg ttttttatca tttcctgcgg tacgtattct acatttccac
    26221 cgggacatcc tccacagtag gaataaccaa caagctgcag ttcttcatct ttcggatata
    26281 atgcaaaacc cccgacatgt tctctcatgg ccctgagaca cttaccacca ccgcaattct
    26341 gataacgccc acagataatt atccctatct tcttcacatg aacacccctt ccactctcga
    26401 gtatctatca ttctcattat agcaagtatt agcaacgata atccttcaac atgcatcatt
    26461 gcctatcttc agaaatagct gtataatttt acggcgttaa tttcatcgga ggtagttatg
    26521 aaggatttca cgaagcgtat gctctccatt gccgtaccaa ttactcttca gaaccttata
    26581 tctaccggcc ttaatctcgt tgacaatttg atgatcgggc agctgggtac aactgccata
    26641 gcttcagtgg gtttggtcaa tcaagtagta ttcattctca acatacttac atttggtgtg
    26701 tccagcggag ccgggatatt tgtcgctcag ttttggggca agagagacga gaagagcatt
    26761 gagaagacca ttggacacat gctatatata accataggag cctctgcctt attctttgtc
    26821 ctgctcttct tcttgcctga gagaattctt atgatcttca cgacagatgt cgaagtggtc
    26881 aagacagggg cagaatatgc aaggcctgtc gctttctcca cattcctaac atccttttcc
    26941 ttcattattg caatggcctt gaggactgtg gaaaaggcta gaattccaat gtatgttagt
    27001 cttgtcgctc tctcgatcaa tacggtggca aattacattc taattttcgg tcttggcccg
    27061 attgaacctt taggcgtttt tggagcgtct tttgcaacgc ttctctctag acttgtggaa
    27121 ttcgtaatat tcgtcagtat tgtttaccgc aagaaaactc caataaggct ttctagatca
    27181 gccttaaaat tcgaaattcc atttttcaga aggcttatga aatatgctac tccagtaatc
    27241 atcaatgaat tcttgtggag ccttggtatt acaatgtatt ctctggttat ggcaagaatg
    27301 ggcacagaat tcatagctgc caggaacatt tccagtacaa ttgagaactt tggttttgtt
    27361 atctttggtg gactttcctc ggcgacagtt gtaatggtgg gagcagaact tggaaggaac
    27421 aatttttctc aggcaaaata caatgcgaga aaattgcttc agcttaccgt catcaccgca
    27481 gtggccacgg gctttattat catccttcta tcgagattta tagtgaatct ctacaatata
    27541 gatcaatcac ttaagaacac tgttctcact gtactaatta ttattggtct ttcacagccg
    27601 atcaagatgt tcaatgcggt aaatatagta ggggttctta gaagcggagg tgattccagg
    27661 gcagcaatga taattgaaat tgtctcacta tggggagttg gaattcccct tgtcgcgata
    27721 acaggtctcg ttcttaaatg gccgcttact ctagtttata ttgcaatgat ggtcgaagag
    27781 cttttcaaat caatcctggg gattaggaga acattaacct ggaagtggtt gaaaaacgta
    27841 gttgattgaa cgcatgcaaa taacaaaggg accgaaaaga attcttcgat cccttgattg
    27901 tcatatctaa aaaattaaaa cgattatgac tgaaggaatc tcaggaacag ctgagcgtat
    27961 attctcgaaa caagaaggag atcttcgatc gatactcttt catttggctg gtgtgccatc
    28021 tcaacatttc caggaaaaag cgctccgaaa gccactgcgt ttggtacggc acgcgcataa
    28081 gtgccaccgc cgatagctat tggttcttca tcatgtcccg ttacttcctt gtacacctct
    28141 ctgcacatct taacgagttc agagtctgga gagatgtaaa gcggcttctg gtgatgaaaa
    28201 gtctcgacgc gaaatccttt gaaggcctct tctatttgtg atctaatcat cgattcgttg
    28261 aagaaaaccg gataccttat attgataact agcttcaata cacctgcctg aagtcttagt
    28321 gtacctgcat tcaaagtcag ttcaccagac atagaatcct ttccagaaat tcctagagat
    28381 tcaccataga actcataacc gatcttggac tttatagcag taagaaagtt tttgatttca
    28441 gagtctccca gtctaagatc ggccagaaga tctacaatag cagctatagc actttgccct
    28501 ttgtaaggag tcgacccatg agcggaagaa cctgtaatca agatttcgac tttatcctcc
    28561 gatcgggtta acttcaattt tgtatcattt ttaggcgaaa aggcttcaat cttctcataa
    28621 ctctcggcat ccacaccccg aagtactgcc ctggccgaag atgcaaccat gttcggagcc
    28681 tcaccggcag aaagaacctc tactgatatt ccttcccctc cgttgtgcgc gggtgctgat
    28741 atcccgtaat taacgatacc tttctctgca aatataacag gaaaatcagc atctggagtc
    28801 actgacatgt ccgggatctc ctcgtgtttg acgtagtatt tcatgcactc ccacccagat
    28861 tcttcgttcg tgccaaaaat cagtcttatc ctcttcgaag gcttcttcac gtactctttt
    28921 acagccttca gagcgtaaag ggctgctatc atagggcctt tgtcatcctg ggtccctctt
    28981 ccccagattt ccccgtcctt tacaagtccg ctgtatggtg gaacttccca gccttctcct
    29041 tctggaacta catcgagatg tcctaaaacg ccgaacgtct ctccagatcc gtaatcaacg
    29101 atcccaacgt atccatcagc gtttctggct tcaaaaccga gatcctttgc caacttcact
    29161 gtcttttcaa gagattcctt aacaccttta ccaaagggac cgccatcaac aggtgtgtcc
    29221 tgaactgact tgaccctaac cgaatcagac acggcactta ccagctcatc tttaagggcg
    29281 atcaccattt cgtcaagttt cctatccact ttttctcctc cttactttat tatctccttt
    29341 atatcttgtg actctcctcg tctgcaaacc aacgttccct cactggttgc taccaatatg
    29401 aagccttcaa ttcccgatac ggctagtttc ttctgggtat gatttatcaa aaggcagttc
    29461 ttcacgtcct ttagagaaaa ctccccctcg actgcattcc cgttcttgtc tttgatcagc
    29521 aaatcataaa ctgcatccca cgagccaata tcgttccagt agaaacttgc cggtacagtt
    29581 gctacttttg aggacctctc catcaaacca taatcaattg agatctttgg aagcctttcg
    29641 tagatctttt ctatgtcttc tggtctctta ctaatctccg aaattgcaga aaacagatcc
    29701 gggaagttct tcgagagctc caaatcgaaa acgctcttct tccaaacaaa gatgccggaa
    29761 ttccatagaa atctgccgga ttggacataa tgctgagcag tttcatagtc tggtttttca
    29821 tgaaacttct ttactgaata tacttcttca ttatcatcaa cagcatttcc ccgctcgata
    29881 taaccataac cggtgtaagg atgagtgggg gtaatcccta tcgtaaccag cgaatcattt
    29941 gattcggcgt acttgattgc cttattcaga gtcttgagga aactctcttc atctcttata
    30001 acatggtcag cgggaacgac aaccatgatg tcatcagggc taaatctggt actgcccaat
    30061 gctattgcgg gagctgtatt tcttctcatg ggctcaaaga ttacgttctc tcccgggaag
    30121 agcggtagat agtactgcat caaaggaaac tgctcccttg ttgttataat gattatctca
    30181 tctgttacct tttccattct ttcaatagtc tcttcaatca ttgtcttcga tgaaccaaac
    30241 ttgtgaaact gctttggcct tttcttaaca cttacaggcc aaagcctctc acctatgccg
    30301 cctgccatta tcaaggtcct taccacatac atctctcctt cccgccgcat cccgttatct
    30361 atagcatacc agaaagctag acctttaccc cgtgaagttc gggaggctat tttcggaaaa
    30421 atcgtgtata acagaatagg aagaaagact tagagacgaa tgttcgactc tacaagcctt
    30481 taatcaccgg cctcaagctt tgcgaccgtc acaatttaga ggcacaatta aatgaggggg
    30541 cgaatatgat gacaaaagtc atgatcaggt tgcgaatgag tcttaacgat gctcactatg
    30601 gaggcaacct agttgacggg gcaagaatcc ttcagatgtt tggcgatgtc gccacagaac
    30661 ttcttataag aaacgatgga gacgagggct tattcaaggc ctatgataat atcgaattta
    30721 tcgcgccggt ttatgcggga gattacattg aagccacagg agagattatt tcgtccggaa
    30781 atacatccag aaaaatgttg tttcaggcat tcaaggttgt acagaccagg cctgaaataa
    30841 acgattctgc cgcagagtta ctgtcagaac cagtactggt atgcagggca agtgggacat
    30901 gcgttgtccc gaaaagcaga cagaggaagc gctatgaata aactcataat tactgctgca
    30961 ttaacgggcg cagaagtgat gaaggatcag caacccgctc ttccgatttc tcctgaagag
    31021 atcgctgatg cagcgtatga ctgctttctg tcaggagcaa gtattgttca tattcacgct
    31081 agggattctt cggggaaacc tacgcagaat ttagaagttt accgagaaat caaggagaga
    31141 atcgcagaga aatgcgattt gatctttcag ccttcaactg gcggagcagt gtggcacaaa
    31201 gtaagagaga gggctcagcc tctagagctc aatcctgaga tggcgactct ttcggcaggt
    31261 acttgtaact ttggtgaaga tgtttttttc aattcacagg atactatgga aactttcgca
    31321 caggagatga aagcaagagg aattaaacct gagattgaag ttttcgagag gggaatgatt
    31381 gaaaacgctt tgaaattgct taagaagggt ttgatagata gccccataca ttttgatttt
    31441 gtcctcggtg ttcctggagc ttgccccggg aatattgaag atctgattca catggtgaga
    31501 gcaattcctc aaggaagcac gtggacggtg gccggaattg gaaggaacga gcttgttctg
    31561 gcaactgccg ctattctcct tggagggcat gttagagttg gtttcgaaga caatatctac
    31621 tacaagaaag gtgagctggc aatttcgaac gctcaactag tcgagagggt tgtaagaatt
    31681 tcgaatgaac tgggtaggga cgtagccagc cccgaagaag caagagaaat ccttggaatc
    31741 aggaggaaga atcagtgaaa ggatgccctt atggaagtca cagagtgatc gagcccgcgg
    31801 gattccttcc gcaggcagcg ttaaggatcg acaactcttt ggacctatac gataatgaaa
    31861 tcctggtgga cgtcacaact ttaaacgtgg actcggcaag ttttactcag ataagagaag
    31921 cctgtgaaga tgatgtgaca aaaatctcgt ccatgattat ggatatagta agggagagag
    31981 gtaagctgca gaatccagtt acgggttcag gggggatgtt tatcggaaaa gtgtcaaaga
    32041 tcgggaaaga ccttttgaga cgagatttag agataggcga tactattgcg tctcttgttt
    32101 cgctatcgct gacaccttta aatatcgaaa ggattgttca agtacatctc gaaaaggacc
    32161 agatagatat tgaaggaaag gcagtgttat ttgaaagcgg aatttacgca cggctaccgt
    32221 cagatatccc ccctaaaatg gccctggcgg tacttgatgt tgctggtgca ccggcacagg
    32281 ttgacaaact agtaaaaaag ggtatgaatg tagcaattct tggaggcggc ggaaaatccg
    32341 gcttactctg ttgttatgca gctatgaaga agtgtcgcga ttcggggaag gttgttgtag
    32401 tagaatattc agtggagaat gcagagagaa ttcgaaagct tggccttgcc cacgaagtta
    32461 tcattgccga tgcaacaaaa cctctcgaag tctatgaaaa aatcactgag tttacacaag
    32521 gcgatctctg tgatgtggtg attaataatg tcaatgttcc cggaacagag atggggtcta
    32581 ttctatgtgc aaaagacaac ggaattgtat atttcttcag catggctaca tcttttacca
    32641 gagccgcgct gggcgctgaa ggtgttggaa aggatattac aatgatgata gggaatggat
    32701 atactaaggg ccatgctgag tttgcgctag gaatcttaag ggaatctagt gaaattagaa
    32761 ggctttttca aaagctatac atttgaggtg gcgtaaatgc gtgactacag agaaatacca
    32821 atgtggagag atgtaacacc tgctcagtgg agtgactggc ggtggcaggt agccaacaga
    32881 gtcaagaccg ttgatgcgtt aaggcagatt atcgacatca ccgaagaaga agctcatgga
    32941 gtcgccgaat gtctcagaac gctacgaatg tcaataacac cttactacgc aacgttgatg
    33001 gaccccaata atcaaagatg ccctatacga aggcaggccg ttccaaccga caaagagcta
    33061 aaaatcgaca aatgggatat gatagatccg cttcatgaag atgaggactc tcctgtacca
    33121 ggactcactc acagataccc cgatcgtgtc ctcttcctta taaccgacca gtgttcgatg
    33181 tactgcaggc attgtactag aagaagattc gctggacagc tcgacagggc gagaacccgg
    33241 aaagaaattg atgatgctat tgaatatatc cgggaaaccc ctgaagtaag agatgtcttg
    33301 ctttccgggg gagacgcatt actagttggg gacgattatc tggagtacat tctcagcgaa
    33361 ctcagggaga tcccccatgt tgagattatt agaattggaa ctaggacgcc cgtcgttctc
    33421 ccacagagag tcactccaga actcgtgaag atgatcagaa aatatcatcc cgtatggata
    33481 aatactcact tcaatcatcc tctcgaaatc actcccgatt caaccagggc atgcgaaatg
    33541 ctcgcagatg gaggtatacc actgggaaac cagtcggtac tcctcagagg agttaacgat
    33601 tctccatata taatgatgga actagtgcat cagttagtaa agatcagagt gagaccgtat
    33661 tacatatatc agtgtgatct ctctcaggga atcggtcatt tcagaacctc aattagaaaa
    33721 ggtattggaa tcatggaatc attgatcggc aacacgtcgg gattgtgcgt tccaacattc
    33781 gtcgttgatg ctccaggtgg gggcggcaag attcgagtaa tgcctcagta caatatttcc
    33841 gaatcagaca gagtcgtcgt tctgagaaac tacgaaggag taatcaccac atatcatgag
    33901 ccagatgaca cttctagcga tgttgatgac agagtgtaca gagagaaata cgagttcaca
    33961 ggagtatcag atcttcttta cggaaactca atgagttttg agcctacgac acttcaaaga
    34021 agagacagga taaagaagtg gaaggagaag aaggtgagaa aatgaaattc gaagacttct
    34081 tcgtgggcca gaatttctca accagaagaa gaattacaac cgaaatggtt gactcctttg
    34141 cgaacttgac cggagacagg aatcctgttc acacagatga ggaattcgca agaaagtcgc
    34201 gttttggcag aagaatcgct catggaatgc tgtctgtggg aataatttcc gcgattctag
    34261 gaaatgaatt ccctggaccc ggctccatat acatgaaaca ggaggtaaga ttcctcaaac
    34321 caatatttct tgaagaagaa gtgttgatta ctatagaaat cctcgaaatc aaagttgaga
    34381 aaaagagatt atctgtaaga acaaccgtag aaaaaaacaa cggagagctg gcagttgacg
    34441 gagaggcgct tctactattc gagggagaat gaagcgtcgg atcagaatat cagatttggc
    34501 cgagggaatt gaagtaatat ccgtgatcgg tcttgagaag aatacaggaa agactgttgt
    34561 tttgaaccaa atgctaaaag aatttcgaga aaagggcaca actgcaggag taacatctac
    34621 cggcctagag ggtgaaaaga gagaccaagt ttatcttaat gagaagccag agataactct
    34681 aagcagaggc acttattttg tcacatctga gagggatttc cgtcatcgaa gtttttctgc
    34741 caccgtaata gatttgagag actacagatc tcctctagga agaacggttt ttgcgcgggc
    34801 ggaagccgat gggaaagtca taatatcggg accatctatt cttgaatatc tgagtgaagc
    34861 tgtaaacaca atgagatcct acggttgtga aaagatattg atcgacggtt caacttcaag
    34921 aatgagtccg ggtacgcctc tagtttcgga cggagttatt ctcgccaccg gtgcggcatt
    34981 atcgatgaat ttggaggaac tgtgtaggaa gaccagtttt gtagtgagat tgctatctct
    35041 tccaggcaca gaactcgagc tccacaaaag aattgctccg ctaggaaccg gtttgtgggt
    35101 agagaaagac ggagttttcg aaagattaag cccttctcca ttaacagcaa aatccttgaa
    35161 gcatgagctt gccgggagaa cgatttacat taatggatcg cttacatctt cagttcttct
    35221 tgctttagca gaaaagaata ctagaatcat cttgaaggat ttttcacgag ttttctttga
    35281 cgagttaact ctcgcaaagt atgaaagagc gggaggccac ctagaagtct tgcatccgac
    35341 gcacctgatc gcagtcacag tgaatccgtt agcaccgaac gggttcaaga ttcaatcaga
    35401 aaaacttata aagtcacttg aaagcaagat tgatgtgcca gtactagacg ttttggagga
    35461 gagttcaaat tgaagtattt ggattgtgga tttgagtaca tcctctctct cattgatccc
    35521 gctactccta tgggcagaga ggaattgcgc gctatgacct tcttgaaaag ggatgaggat
    35581 atagaggctt ttcacaagag acaacttgag gtgaacgaga gaaaatcgca aatagagccc
    35641 ctcaagtcta ttctagcgag ggtacgtgac ataaggggaa ctatttcaaa tctctctcct
    35701 gggaagattc tcgacgatat tgaactgttt gagataaaga gcttcgccta ttggtcagaa
    35761 agactgagaa gtgagattgg agagtgcagt tcgtggatgc agcttcccga cctgtcagtt
    35821 gtgttttctt ttctagatcc cgacgatgtt ggaacagaga gtttctacat ttctgacaag
    35881 ttagataatg ctctcgcggt tgtaagaaaa aagctttata gacttcagag aaatagagaa
    35941 gagtatgacg aaacgagtct caatgaactt atccgtctga attttgaaat cgaagagaaa
    36001 gtgagagctg atctttccgg caagctcatc acttatcgag aagaccttca aagctctgca
    36061 acaacaatcg ggaagattga cctgatcgta gcgctagtag agttcaatag gaaactcggt
    36121 ctcagaaaac cggaaatcag ttcaggagaa tatgaatttg aagaactggt caatcctgaa
    36181 ttagctgcat cccttaacaa gagcggcaag aattttcaac catttagtgc cagttttgac
    36241 agaagatccg ttattatcgg aggcaacatg acaggcaaat cggttcttct cagaactgtt
    36301 gctctccttc agacaatgtt tcagcatgga cttttcgtgc cagcaaagag agcctttatt
    36361 cctgtatatg atgcgattgt ttactacagt ggcgataaac agtctttttt ggatgggctg
    36421 tcatcgttcg ccggagaagt agaccatctg aagaaggctc tagagatagt ccatatggat
    36481 atcagaggcc tgtttttgtt tgatgaaata ggaaggaata caaaccccgt agaaggcccg
    36541 gcatttctta tggcttcggc agagtatctt agtcgaaatg agaggtgtcg aaccatcctt
    36601 acatcccact atgaagaagc cctggagatt gaaggagcga agctgtttat tattaaaggg
    36661 cttgaccatg aaagaatgag aaatgaaatc ggaagggaaa tctcttatta tgtggatcat
    36721 tcacttgtag aaatagaaag caatgcggcc tttcctaggg aggcagtaac aatagcgcgt
    36781 cttatgggat tagacagaga gttcgttgaa ctgataataa gaaagctgaa ggagagttgc
    36841 gaatgaatgg aaagctgaat ctgaacatga acaaagtcaa agaagcaaga gattatgccc
    36901 aaaggattgc cagcgaagtt catgactttg ttgaagagca cacaacagta agtatcgaga
    36961 gggcgatctg caggtttttt ggtatagatg gtgttgacga ttatggaatt ccaatcccaa
    37021 atgtcgtagt agatcacctt aaatcatcaa acctaattaa tgatggtgct gcctatgtga
    37081 tcggaaatgc gattctcgaa acgggacttt cgccgcaaaa gattgcagaa gaaatctcat
    37141 tcggaagact cgatctcaga agtctgagtt acagatctca agatgaagtt ttcgatcgca
    37201 tcgaccgact cgtgaatgat gctgtaggaa aagtcaagtt gaatcgagaa aaacgtgagt
    37261 ctttgatcgg tgaactgggt gagggaaaaa agccctacct gtatgtgata gtcgcgacgg
    37321 gaaacattta cgaagatgtt attcaggcaa gagcagccgc aagacaaggc gcagacatag
    37381 ttgctgtaat caggtctacg ggccagagtc tccttgatta tgtgccttac gggccaacaa
    37441 ctgaaggatt tggaggcaca tacgccacac aggagaactt cagaataatg aggtcggctc
    37501 tcgatgacac cggaaaagag gtcggtaggt acataaggct ttgcaattac tgctcgggtc
    37561 tctgcatgcc ggaaatagct gcaatgggag ccatcgaaag actagatgta atgttgaacg
    37621 acgctctcta cggaattcta ttcagagaca taaacatgaa gagaactttg atagatcagt
    37681 atttttcgag agtaataaat ggatttgcgg gcgtcataat aaatacagga gaagacaatt
    37741 acctcacaac ggcagatgct tttgaagaag cccacacagt actcgcttct cagttaataa
    37801 atgaaagcct ggctctcatg gcaggtatac ccgaagagca gatggggctc ggtcatgcct
    37861 tcgaaatgga tccatcaatt gaaaacggtt ttctgtatga acttgctcaa gcacaaatga
    37921 gtcgagagat cttcccgaag gcacctttga agtacatgcc ccctacaaag ttcatgactg
    37981 gaaatatctt tatgggctac gcccaggaca ccttattcaa tgtgatatct ataatgacaa
    38041 atcaagggat tcagcttctg ggaatgctca ccgaggcaat tcacactcct tttatgagcg
    38101 ataggtacct tgcaatcgaa aacgcaagat atgttttcaa caatctacgc gatctaggcg
    38161 atgagataac cttcaaagac aacggaataa ttcagaccag agcagcgacg gttcttgagg
    38221 aagctcttca actgctaagg gaaattgaat cgataggtct ttttgaaacg ctcgaaagag
    38281 gcacttttgc aaacatcaag agatccccac atggaggaaa aggtttggat ggggtagtaa
    38341 gaaaggggcc aaaatacaga aatccattta tcgagaaaat gcttgggggg cgcaatcatg
    38401 gataactgct ctgaaaagaa gaatcaattt ccctctgaaa tagacttaac aagagtaatt
    38461 ccatacggag acacgctgaa tgacggcaaa gtccagctca gctttacttt gccagtgccc
    38521 ggagggcccg aggccgatga agcagctaag caaatgatac acaagatggg gttgaaaaac
    38581 ccgcaaatcg tcttttcaaa ggaaataaca ataggattca cattctttgt cgcctacgga
    38641 gagttttctc acgctattga ctacacagca attcgagtgc cgaaaattac gcttgaaaag
    38701 atgtctatgg aggagatcga cagatttatt gaagagagaa taaagagaag aatcgttgtt
    38761 gttggtgcta gcacaggtac cgacgctcat acagttggta ttgacgcaat tatgaatatg
    38821 aagggttttg cggggcacta tggcctcgaa cgttacagaa atatcgatgc ctataacctc
    38881 ggcagtcagg ttcaaaatga agagttagtc tcaaaggcga tagaacttga tgcggacgca
    38941 attcttgtgt ctcaaactgt tacggccaaa aacgtccatc ttagaaacat gacttcgctt
    39001 gtcgagcttc tcgaagctga aaacataaga aaaaagatca ttctgatagc cggtggagcg
    39061 agaattactc atgaattggc caaagaactt ggatatgacg ccgggttcgg ccccggaact
    39121 ttcgctgaag atgttgcggc cttcatagtt cacaaactgg aatccatgat ctgaaaagtt
    39181 gtgatataat caaccatcgc ggcataggcg gaggtaacac gattgaagac cctcgtagta
    39241 gttaacccaa atgctagtca cggtgaagcc ggcaaggact ttgaaaaaag catttcctca
    39301 ttgcttcgag aggaaatagc tgactacgac atccacatta ctactggtcc tgaagatgct
    39361 ctaaatttcg tgacaaaaaa cccgaactac gatcgaataa tcagcgtggg aggagatggc
    39421 acactaaacg agatcgtaaa cgggatgata aagggaaaat cgaatgcttc ggtaggcatc
    39481 gtcgctctcg gctctggcaa tgatctcgct agaactatca atctaaagca caattatcga
    39541 gaaatggtca ggatagctgc gggcgaaaac gttcggaaaa tcgatctgct caaggtgtcc
    39601 tatgtagaca tgaacggcgt gaaagcttca cggtacgctg ttaatgttgt tggagccgga
    39661 tttgacgcag caattacagt tagaatgaat aaatcaagat tcaagacaag cgggaagatg
    39721 gcgtacctgc tttctttttt gatggaattc ttcacttcaa aaacttatcc tttaactttc
    39781 attgtcgacg gagaagaaac gtcagacagc tacttcttct tgaccatggc caacggaaat
    39841 tactttggcg gtggaatgag gatagcaccc aaagccctgg tggatgacgg atattttgac
    39901 gttgtgggtg tttcaaagat gtcgaaattg cgtctgcttt atcactttcc gaagatctat
    39961 cgaggggagc atctggtagt tgatacagta agctatcgaa ccgccaaaaa catctctttg
    40021 aaaagcgaca gagatgtaat tattcagatg gacggagaag tcgtaggaag cctacctatg
    40081 gaaatctcga tttgtgaaaa ggctttgaag atagcttcag cgtaagacct tcaaattcat
    40141 tattgcgctt tccaaagtaa tgtaagaact gaaatcaaat gcaacacttt cgaatacata
    40201 ttctccatcg cctgtataag gtaaatccgt tttgacgttc tttattgcag cgctagtttt
    40261 aagaatcgct cgaatattct ccggtatgtg gatctctgaa ttgattatcg cggcattgac
    40321 cttcatattc gtaaagcccg ctgctgacat agaaacaatt gccttgacga tcgctccgtt
    40381 aattctaagt tcatcaactc taagctctcc tagacttaaa ttgcagttca tgacagcccc
    40441 gttaaacttt agaacatagt cataagcggg attaagtttc ataacgactg tactcgttcc
    40501 caaccatgaa acgttcgatt ccggtttcat tatgaacctc tgcgacagct gagtaatatc
    40561 ctgttcaatt ttaagacctg aaccacgtac ggcataatcg accacggcaa cagtagaaga
    40621 tgctacttca tctctcaagg gttccaaact tatgttcgac attgcgaggt cgagctgaat
    40681 ttcaatgctc gttgcagaat cggaactctc caatactacc gtatcgctaa cggccttctt
    40741 accggcaaga aatggagagg gccagaagaa aacgaacagc acaaaagcta caataagtat
    40801 tgtattcaag tatctgatcc acctgagtcc cctaatgcta gcaagtatgc tcattcctac
    40861 cagaacaaga atgatcggcc agaaattcgc caaattgacc aaagcattta ggagagtgtc
    40921 tcccgtaaat agaccgaaga tcagcaaaac accaaccagg acaaagatca atcccaaagc
    40981 aatcttcata ggatcatccc ctgtttattg atttatagat cacaaatcct ccacctgcaa
    41041 tcaatacaac acctataacg gttttccaga agataccgct gaaaatgttc cagggagtaa
    41101 atgatcctaa aagaagggct atcccaaata ctatgagaag cgccgcgatg atcagtttac
    41161 tcttgctatc agagaattct tccttttttc cctcttctat cggcttcccc tcttcatcca
    41221 ttatcacgac gttgttctcg ctattgatag gtgccctggg tactataaac ataccaatta
    41281 tgtatataat caatccggca ccacctgcga agatcagagc aacaaacacc agccttacaa
    41341 tcaccggatc tacccccaga tactctgcta tcccacctgc gagaccatct attactttgt
    41401 cctttctaga cttgtataac ctcttcgtat cactcacata accacctcca ttaatgctca
    41461 agtaagtaac cgtataactt tgatctcatc cattatagta cgatcgatat ttttctagca
    41521 taaccggcta gatcattcac taatctttaa cagaaaaggg aattagcact tacttgttcc
    41581 aaaacgtctc gccgttatcc ttacggattt gcccgttaag tgaaccgcgc gactgcagaa
    41641 atggatgcga ttggatgaga taagagcact taattacctt tcagcataaa tgatcagctt
    41701 cattcgaaga aaaccgagtt ttggcaaata aaagaatgat gatacactgt agttaaacga
    41761 ttaactaaac gtttaactgg caagccagaa ggggtagatt taaatggcga acatgaaaga
    41821 tgttgctaag cgtgcagggg tatcggttgc gacagtgtca aatgttatgt ctggcaaaaa
    41881 atacgtcagc ccggagctaa agagagctgt tctggaatcg atagacgctt tacagtacag
    41941 accgagcaaa atagcgagaa gcctcaaaat cagaaagtcc tttctcgttg gtctcttggt
    42001 tccagatata acaaacccct acttcgccga gattgccagg ggtgtagaga gcatagctct
    42061 cgagcacgac taccagatgt ttctttgtaa tagtgatgga agaagttaca gagaagaaga
    42121 aaccttgatt tcttttcttg gacatggagt tgatggtgtc ataaatgtgg caccgagaat
    42181 ggatgaggag cttctagccg atctctccaa tggaatgcca atggtaatcc tcgataggca
    42241 cctcaaaata gaaaacccgt taattgatgt agtatacacg aataacttca agggttccgc
    42301 tcaactggca aggcacttgc tggacaaagg ccacaagaga tttgcttgta tatctggtcc
    42361 taaagatgtt ccaagcgcaa tgaggagact tcaaggcttt tgtagcgaat tagaaaagga
    42421 cggtattaaa ggcgaaaacc tgaagattta tttcggtggt ttcaagtacg aagatggttt
    42481 caagataatg aagcaattca ttttgaagct taaaataagg cctacagccg tttttgctgg
    42541 aaacgacatg atggcgtggg gagcgatcga ggcggcaaaa caaaatggtt tgaaaattcc
    42601 cgaagatatc gccatagccg gcttcgacaa tgttctgtat tcatctctgg taattccgtc
    42661 aatgaccact gtcgatcaac ctaagttcga agctggacgg acagcgatga aactgcttct
    42721 agaaaagatc actaagaagc aacatggaga atacgtcgac acaagaaaaa ttgaactgga
    42781 ttcacatctt attgtaagag aatcaacgca agtttgacaa atctttggag gtgaaatgat
    42841 gaaaacaaaa ggcattcttc acaacgatct ttcgagagaa gtcgcgaggc ttgggcatgg
    42901 tgacatgctg gtaataacgg atagaggttt tccctttcca aggcatggga acactgtatg
    42961 cattgacgtg tcagtcggaa gaaatctacc aagattcgtg gatgtcgtta aagtcgttct
    43021 tgaagagctc gagattgaaa aggtaatcat tgctgacgag accaggtcag taagccccga
    43081 gatctattct cagctaaagg agatagtctc taaagtgaaa aacaagggca acgacattat
    43141 cgaagagaac attcctcatc cggagttcaa ggatctcgtt ttgaatggaa gtctccaggg
    43201 caaagaggta aaagtcttcg ttaagactgg ggaattcacc ccatatgcaa acataatcct
    43261 ggtttccgga gtcgatttct agatttcggc aaaaggctca gttcaagtat cacgatttca
    43321 tctgcgccaa aagaaaaaga ggtgattatg tgaaaaaaaa cagggaagtc ctgagcagcg
    43381 tatttaaaaa agaaaaaccg attattggaa tggtacatct gcgtcccctt ccaggatcac
    43441 cgagatacga tcccgaaaaa ctgggcatga aggaaattct cagaattgcc gttgaagaag
    43501 cgagaattat ggagggatgt ggcgttgatg gacttcaaat tgaaaacatc tgggactttc
    43561 catatctcaa aggggaaagg atcggtcacg agacttccgc tgctctggca gtcgccactc
    43621 aaagtgtgag agaagccgtc aatatacctg taggtgtgaa ctgccatctc aatggaggaa
    43681 aggcagcact cgccgccgca gttgctggtg gcgcaaaatg gataagaatc ttcgaatggg
    43741 tcaatgcgta tgtttctcac gcgggtttga cggaaggcat cggggcagag ctttccagat
    43801 acagaaaact tataggcgca gaagagatta agttcatgtg tgatgtgaat gtgaaacatg
    43861 ggagtcattt cattatttca gacagaagtg ttgaggaaca ggctcatgac gccgaatcgg
    43921 aaggggcaga gattctaatc gttacgggat ttgagaccgg gcaggcacct aacgcagaaa
    43981 aagtaaagag tttcagtgaa gctgttagca ttccggtgat gatcggaagt ggtttgacaa
    44041 aagaaaacgc aggtgaactt cttaagtttg ccgatggagc aatcgtcgga agctacttca
    44101 agagggacaa caactggaag aatcccgttg atccaaacaa cgtcagaagc tttatgaaaa
    44161 ctgttgaatc ccttagagag gcgggttacg atgattgatt acgcatttaa cggagagatt
    44221 ctctgcatgg gaagcatgaa catggatcta gtgatggtaa tggatagact tcctgagcct
    44281 ggagagactg ttcttacaga caacttcagt acctacccgg gaggaaaggg aggaaatcaa
    44341 gcagttgcgg cggcaatcag tggcgcgaga gttcagatgt ttacgaaact tggtggtgac
    44401 ggattcagtg aagaactgat ccagatgatg aaggaaaaag gggtagacac ttccagaata
    44461 cttagggatc ccgaaagcac agcgggtatt gcaatgataa gagttgacaa gaatgggcaa
    44521 aactcgattt cttttacccc gggtagtaat gcgcggcttt ctcctgaaga cgtggaggaa
    44581 aactcagatc tcttcaaacg agggcgtatt cttttgttaa caatggaaat agcacaggag
    44641 acaatcttca gggcaatcaa actggcccat gaaaatggaa tgtttacgat cgtcgatccc
    44701 gcgcctgctc ctagaaatgg gttccccgat tccatcgcgc ctttgatcaa tttaataaag
    44761 cctaatgaat ccgaagcgag gattctgaca ggcatagatg tccacgacag agaatctgcg
    44821 cggattgccc tccaaagact aagttcaatg ggcttcaatc taccaataat tactcttggc
    44881 gaaaaaggcg ttgttggagt tgatggcaat gacttcatgg acttgaagcc ctttgacgtt
    44941 cactgtgtgg acacaactgc ggctggagat gtcttttcag gggctttggc agctgcttta
    45001 tcgaagggta tgcatctgaa ggaatctcta aagtgggcaa atgcggcagg agcactgagc
    45061 accacaatct ccggtgctca aacttctatc cctgctccga aaagcgtcga gaagttacta
    45121 agaaggcagg tgtaacaggt gcccgatatt gcgctgaaac tggaaggaat atataaatct
    45181 tttggtggaa tccatgctct gaaggatgta agcttcgagc tgagcgaagg tgaagtacat
    45241 gcgctccttg gcgaaaacgg agctggaaaa tctacactga taaagatcct gaccggagtt
    45301 cataaaatgg attcggggac tattcaggtc caaggaaaaa cagtaacgat tgaagacccc
    45361 gtagacgcac gacacaaagg aataggcgca atatatcaag aactaagtct cgtggactca
    45421 ctaacagtcg ccgagaatat attcctgggt cacgaaccta tcacatcctt ctttggaact
    45481 ttaagaaaga aagagctcat tcgaagggca gcggagtatc tatcttcctt cggaatcgaa
    45541 atagatccca gcacttttgt cggagacctc ggtcttggac agaaaaggat agtggaaata
    45601 ataaaagcgc tatcgataaa cgccaacatc cttcttctcg atgaacctac gaccggtatg
    45661 agtagagtgg aaatcgatac tctgtttgag attatggatg atctgaaatt aaaaaaagtg
    45721 aacatgatct acatctccca tcatcttgat gaagtcttta gagtgtgtga cagagccacc
    45781 gtactgagag acggtcaaaa cgccggaacc ttcgaaattg ataaagtgga tctaaagacg
    45841 ttggtaaaat caatgcttgg aagaaccttg aaagaagagt tcccggtacg agaggggaga
    45901 ccatccgaag aaacaatatt ggaaattgaa gagttcaaga ccgacaagat gcttgctcca
    45961 gtcagtttta gcctgaaaaa aggcgagatc ctcggtataa cgggaataat cggttctgga
    46021 aaaagcgaac ttgccctcgg tttgttcgga gcagacaggg ctgtaaaggg aaagctctca
    46081 attgctggca aggaaatgag attaaactcg cccgatgatg caaagagaca tggtatctct
    46141 ttcattccag aggatagaaa ggaacagggc ttatttctta ggctcaatgt ggaacacaac
    46201 atctgtgttg caaacatcga tctcgccatt aagaacggaa taatctcgag caaaaagaaa
    46261 tcggaaattg caaaggagac atcagaaaaa ctaaaaataa gacctttaga cataaaaatg
    46321 gcagtccaga atctctctgg tggaaaccag cagaaggttg ttattggaaa atggctttgt
    46381 ggtgaaccag atatcttgat acttgatgag ccgactcgcg gaattgacat cggtgcaaag
    46441 acagagatct attctctgat caactacctt gccgatagtg gtacaggaat attgatattg
    46501 tcatctgaat tccgggagat gagtgcgata tgcgatcgaa tcctagtgat aagaaaagga
    46561 acagttgtcg ccgaacttga aggaaaagat tcttctacag agcagttgtt gacactggcg
    46621 cttggaggtt aatgcacatg gaaaatgaga ctgagaaaaa tgacagatcg gcgtttgtta
    46681 gcaaattcct gagattgctc aagggtgaat tagggctgat cttgatcctt gtagcattaa
    46741 tggtggtttt ctcattgatg tcaccatatt tcttcacttc tagaaacctg ctgaacatta
    46801 ccaggcaggt gtcgataact ctaattgtcg ctattggtat gacctttgta atactcacag
    46861 gtgagataga tctttctgtt ggttcttctg cagcattagt tggagttgca actgcggcgg
    46921 ttcttaatgc gacgggctcc atcttcctag ggctgattgc ggggttgata cttggggtcg
    46981 gaatcgggtt agcaaatggc gttctgactg tttacggtcg agttcagtct ttcatagtga
    47041 cacttgctat gatggggatt gcgagaggag ccgcgctggt ttggaccgga ggaaagccga
    47101 tatccagatt gcctgtagat ttcgcgataa tgggtgctgg ctatctcgga ccagtaccgg
    47161 tttcaacgat aatagctgcc ggagtcttca ttctcgcctt ctttgttctg aacagaacaa
    47221 aacacggagt gtacgtaaag tcaattggag caaacaggga agcagctagg ctatcagcaa
    47281 taccagtgag gaaataccgg gttcttgcct tcgtcatttc cggtctcatg agtgcacttg
    47341 gtggtattct cataacttcc aagcttcttt ccgctcagcc aactgccgcc gaaggacttg
    47401 agatgaatgt aatagcggct gtcatacttg ggggagcatc tctttccgga ggtgttggta
    47461 cagttctggg cactctcctg ggtgccatgg taataggagt tatcgataat ggaatgaatc
    47521 tcgtaggagt atcatcgttc tttcaacaaa tagtgaaagg agtaataata ctcgttgccg
    47581 tactggcaaa acgtagtgat taggattctc tatcaaaaaa aggaggggta acatgaaacg
    47641 aatgtttctg gtagttctta ttatcggatt gttagcgcta acgggctttg ctgcaaaaac
    47701 catcggtttt tcagtctcga cacttgcaaa tccctttttc gtaactatga aggaaggggg
    47761 agaggcaaag gcaagcgaac tgggtttgga gatgatcgtt ttagatgctc aggataagcc
    47821 ggaaactcag tttgctcaga ttcaggatct cataacgaga agagtagatg tgttgattat
    47881 caaccccgtt gactctgacg caatagtctc ggcagttctt gaagcaaacg cagccggaat
    47941 tccggttatc actgtgacaa gaccttcaaa cggtggagta gtggaacagc atctagacat
    48001 cgataacaag gaagcaggta tgctcgccgc agtagcattg gcagaagcac ttggcggaga
    48061 aggaaaagta gccatccttg agggaatacc cggagcaccg tcggcagttg acagacagaa
    48121 aggtttcatg gacgaactta agaagtaccc aggtatcgaa gtagtagcat cactgacagc
    48181 caattactca agagaagaag gtgcaagagt tacggaagac attcttcaag gtaacccggt
    48241 gctggacgga atctacgcac acaatgacga gatggcactc ggcgcggtta gggcagtgat
    48301 tgccgcggga agactggacg aaataaagat tgtcggaatt gatgccacag atgatgcaat
    48361 tgcagccata aaagccggtg aaatggttgc aacagttcaa cagcagcctt acctacagat
    48421 gcagatggca gttatcgcgg cacagagaat catcaacggc gcaactgtgg aacctaaggt
    48481 aataattcca ctgaaactta taactatcga cgttctcgag taattagctg atctacaagg
    48541 agagcagcgt acgctgctct cttttttttg tgagagcatc acgaagaaaa tgaagagccc
    48601 actcatctga tttctcaaaa tactcgcttg cgatgatcca aaactcttcc attttgtttc
    48661 caagctacta aagaaacaat agagatctag gagacgactt ttgaacaatg gcccccttaa
    48721 tttcaagtta acaatgctgt tcagctatat cgaatctttg ctttcagaat accacaagga
    48781 gtataatgtg actgattagt catatgactt gtcggtcaca aaggagatgt tagagtgcca
    48841 aagggaactt tcttcaactt gtcggaaacg aagcgaaaac gaattttgga agccgccata
    48901 gatgagtttg cagagaatgg atatgaaaat tcaagagtca acgagatagt ccgaagatct
    48961 tcaatcccga agggtagctt ctaccagtac ttcgaaaaca agtctgattt gttcagatat
    49021 gttctggaca ttatctacaa gaaaaagatg gagattatga cgggcatcga aatctcacac
    49081 agagaactga gtgtatttga aacattaagg gtaatggttg aagcaggaat taagatggcc
    49141 gaggaagatc ctcggctttt gaagatcagt gacagtctcg ttgcaaacaa acctttgatg
    49201 gatgaggttc tgatagaata tgctccatca agcgatgatt ttattatttc cttgattaga
    49261 cgaggaaaga ataacggtga attagctgat tgggtggatc cggagctatc tgcgaagttg
    49321 attactgcct tcatgctctc actgagtgaa atgataagat ccagcagcaa tgggaagatt
    49381 actgacgaaa cgagggagaa gtacctctct ctaattaatc ttcttgaata cggtatgaaa
    49441 aggagagaag actatgattc aggtatgtga tctttactat tcttacggga aagacgaaga
    49501 gttcgccgta aatggcatct ctttcgaagt caaggaggga gagattttcg gttttctcgg
    49561 tccaaacggg gcaggaaagt cgacgactca gagaatatta acgggcctct taccggtcca
    49621 aaagggaaaa gtaagggtca tggatgtcga tgtttctgca actacccctg acttctataa
    49681 caacataggc gtctcctttg aaaatcctaa tctttatctc aagctgactg gtatcgagaa
    49741 ccttgagtat tttgccaatt tgtacgatgt accaacagaa gatccagccc atatgcttga
    49801 catgatcggc ctgaaagacg ttgctaaaaa gagggtagga gaatactcca aagggatgca
    49861 acaaagactg gtctttgctc gttctctcat taacaatccg aaag
//
LOCUS       DAOQYI010000014        44789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_942_length_44789_cov_71.728618, whole genome shotgun sequence.
ACCESSION   DAOQYI010000014 DAOQYI010000000
VERSION     DAOQYI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 44789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44789
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_942_length_44789_cov_71.728618"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKL93_05640"
     CDS             <1..195
                     /locus_tag="PKL93_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70780.1"
                     /translation="YPICSSIYLQYPCGVNMFFSVNGEPLNGQPVSLDFPAPDAGLTT
                     HKLHLGTNLGFHILFSAIET"
     gene            complement(165..1217)
                     /locus_tag="PKL93_05645"
     CDS             complement(165..1217)
                     /locus_tag="PKL93_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNQ70781.1"
                     /translation="MKRKILIAFVGFLVILFLVSSCIPFPKVKNYRAWVVGQTDTNGI
                     AMLYFSDDSGETWTRQAMDILPEGKSLEDVLSVDENTVWACGSDGLLLKTTNGGSDWE
                     IVGVSEVATDSFFSFLSIFEDRIWVSGDEGLVIFSDDDGGSWTVCDLPEMASEYIIQG
                     IHAINEDVIYAVGNKSTPRAGIVLKSEDGGQTWEEIELPNNYNDNGWIGVKATDENHI
                     VIHGGQGHYVVTANGGKQWVTGGPLFAKDLNSLVMLDSSTYWAACDFDTIILTENSGI
                     SWEEQPPAGTSNSFLLGIDALDRSNALVVGSSAGYPQFGKILRTKDGGKNWEVVLSAE
                     ECPVTLTHVSIAEKRM"
     gene            1595..1738
                     /locus_tag="PKL93_05650"
     CDS             1595..1738
                     /locus_tag="PKL93_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70782.1"
                     /translation="MKSVSIPVIYIDERLAITDGHTRAVAAIIEGQDSMLVEITDEKL
                     DGI"
     gene            complement(1938..3119)
                     /locus_tag="PKL93_05655"
     CDS             complement(1938..3119)
                     /locus_tag="PKL93_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ70783.1"
                     /translation="MIRLLSGSASVGIIYGIFNFVLIPYLNGKFHSTALAGNLVSAGM
                     IITIFTGMLIGYLGDRNRKYLFFIKGLFIIAIASMFMLSTGNEILLGIAAVVFTMSIF
                     SLLTPYSALVSNVSKPGRKDRNYGFMMGVMNIATFLCSLFIGVALSRSEETAFVIFSI
                     SAAVLVIPIFFFRSGSQVDDAAVGIREEDLRFRIDRKIVFVMISQFGLWFSMGGILPY
                     LTSFISSDIGVSLGTASSIMGISTLVSGVTSLMTGTLSKMLGQRRLHITALSILAGVF
                     LLVSVFYTGFLDLGPFLMLIVLILFSFALGIVLALNVTIVSDTVSLANQGKIFGLNNI
                     VMVLSQSLALSLIGSLIALGGYNLAFVFVAGGFAAAILFFVISTLSYQGPLNEQKAGK
                     T"
     gene            complement(3112..4092)
                     /locus_tag="PKL93_05660"
     CDS             complement(3112..4092)
                     /locus_tag="PKL93_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNQ70784.1"
                     /translation="MNRIFRALEAFAVGDAMGMVTEFMTRRQISARFDFVSDLLEPEH
                     SPIHKNLTRGQITDDTEQVLYLIKLYNEKRNFLQETVKEALCRWADERDPIAKGYIGP
                     STKRAIEAFKNKEEFESLGTTSGAPMRVLAPVLCSLNKSEKHLIEVIIECTVPTHNTN
                     LALEASLAVGFAYYYAAKGQGVNGIIEAAIRGSRLAEELSCAEFVGPSAGERLSLIRD
                     LIGGELPDHFLDRIYYLFGTTMEAVDVAVAAIAIGLFCRDDVWQAIRMGASIGGDTDT
                     IAAIAGSLSSLQGETNNIPHFITQRVLKTNENLDLEKYVRYVEETSDIDD"
     gene            complement(4089..5060)
                     /locus_tag="PKL93_05665"
     CDS             complement(4089..5060)
                     /locus_tag="PKL93_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNQ70785.1"
                     /translation="MRIGLVYNPNSGKREKDLDLMKALAKKFEKLHLFCTKSTHLHLK
                     EYFNLEVTGSGTIHNDYRDTINAGNALSKMNLVISIGGDGTASDVVAGMRLTGRLAPV
                     AGVALGTANCGPLIVFRSAADILDFDFLNIEPKWVTGLDVFEEDYISSAFNDVVISET
                     LISTVEGKNLTIDASNFFYRNERTPREPVSIASETSRLEINGEEVSPHFRTSQIIISP
                     FSKDTKGLFAYKAVSGLLCWLPYSSCNSAMIVSSRPIITMVEPGETVSAEIKQFILTN
                     SDTVSLSGFEAFCVIDGNPRVDLSASKGIKVKINGRAGLGVKRRDEE"
     gene            complement(5062..5997)
                     /locus_tag="PKL93_05670"
     CDS             complement(5062..5997)
                     /locus_tag="PKL93_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HNQ70786.1"
                     /translation="MADRDTMMKVCKLYYIEDKTQSEIARLVGISRPQVSRLLTKAKN
                     EGVVRIEIDSGKVENLEEISIEMKERFGLKNVIVADEASDGGTINSIAMSAAKFLPDY
                     VKNGQLVGISWGRTLYETVERVVFNGELPNTTFIPLIGGVGQLRHEYQMNSIVEKIAN
                     SFHSNRYYLFAPAFIENAKTLNMMLEDSSIRFMSEMWKRLDLAIVGIGEPISLSNAFK
                     NIYDKEFLANLMKHAAVGDIAARFFNADGTPCVSGNENILGISLDQLKEVPEVIGIAG
                     GKEKAQAIYASIKAGYINSIVTDRSTALQILRMQS"
     gene            complement(6006..6839)
                     /locus_tag="PKL93_05675"
     CDS             complement(6006..6839)
                     /locus_tag="PKL93_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNQ70787.1"
                     /translation="MRIAVIGAASIDRYVILDSFPERDSMVFARSTHDFIGGSGANIA
                     LNLSRRVETDFYFGTGNDLSSKWILEKLSLSSMNLSYVVENGSGAETLVMIDSHGERR
                     IISLGGHALFRGVIDEKRYNAICVADSFKEVALDAFRKTAESLKVYVPGGCGLYFGAD
                     TVVEVACLSDYTILSEGEAENLQTRISEISSNIIITRGSAATIWMDRNKNSRSFEVEG
                     IAEEILDTTGAGDAFAAGFMEKFTKCGDPIQSIYYGHKKAAEAVSVIGPNLVEGRKDL
                     R"
     gene            complement(6836..7663)
                     /locus_tag="PKL93_05680"
     CDS             complement(6836..7663)
                     /locus_tag="PKL93_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70788.1"
                     /translation="MRPKRSVVITMNIVAFLVVLLILSPLLMLILNSFRETTDIYQNP
                     LKLPEKFYLGNYSKIFQDTSFGRNFTNSMTVTLVTVLLCIIITTLAGYAMSRFKFKGK
                     NPLILWLLASQAFPGILMIIGLFSLLNRYSLQNNPLGLIILYTTFTIPFCSWLLKGYF
                     DEIPQALEEAAMIDGCNRFQAMRKIIVPMAVPGIVAVGTFAFLLSWNEFFFALVIMRE
                     NANCTLPVFLSRFVGTGGAVEWGALSAGALITALPPILLFLLSQKYLIGGLTRGAIK"
     gene            complement(7653..8492)
                     /locus_tag="PKL93_05685"
     CDS             complement(7653..8492)
                     /locus_tag="PKL93_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70789.1"
                     /translation="MPYILPVLVVVALISVYPFVNGIYNSFFSDAVFGGGKYFSGLDN
                     FKDLVSDNLFWTGLKNNLIWSAACVGFELVLGMLIALLLNLPYIRLRGLYRALILLPW
                     ATTPVVAALIWRYIFGVMGPLNSFLNAVGFANPPNWLVTPGYSLFACIVANIWIGLPF
                     VIVTLLAGLQSLPSDVYEAAEIDGAGVFKRFFHLTLPLMKSLILILVTLNTIWTFNMF
                     DIVYSMTNGGPGNSSMLLSLYSYQNAFAYFQQGYASAIGVICLLILLLPVAFYIREVR
                     NEA"
     gene            complement(8709..9962)
                     /locus_tag="PKL93_05690"
     CDS             complement(8709..9962)
                     /locus_tag="PKL93_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ70790.1"
                     /translation="MKKVFVISLLLIITAAVFAITLDFYCLAWQPAAVDKIYDLVDIW
                     NMDNPDVQVNIIWGTWESSDQYLLTSFQGGNAPDVFHTDAEKFREYGMMGYAAPLNSY
                     VSDEIIGDIPAHIFEDCYDFAGNLYGIPWCQETQVIFYNKDIFEESGIRLPLDRMISW
                     EELLDIAQRVTKKDENENVITWGILAPLMERFHWTLIAQKDGTILTKDSNHKWKVEIN
                     EGAIEAIEFYLSLITEHQVMPRDVISIDYTSLMQGFINGKYAMVTFGCWNRRFLLQNK
                     GFNWGMLLVKNEDNRINASDPQAFGISKLSKHKDEAFAFIEFMTNTENSAEISYSDYL
                     FPVRESSLEDPRFSSPENEWDVAKAWLQYSDNVKPGMPGFYSFEWKLFVPNLEKVILG
                     NMSLDAALKDTVTEGNKMLKKLGLQ"
     gene            complement(9974..10984)
                     /locus_tag="PKL93_05695"
     CDS             complement(9974..10984)
                     /locus_tag="PKL93_05695"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNQ70791.1"
                     /translation="MKAAIYKGIGKIEIDDISKPVIQADEGLVKIGMVGVCGTDIKTF
                     NKGHHMFKPPCILGHEFTGKIVEKGASVDYDFGDASYVIAPYIECGKCEMCQKGVPEL
                     CSKKHWVEGAFTEYVKVPLEILKRATLKIPNHVPESTATLTEPLACAIHGIDKARITA
                     EDRVLVVGSGPMGLLLGNALKNMKCETYISDIDKNRLSMAENCGLKTIDFSESGFESF
                     KNSNPRFDSIILANDRKELVNSLLPFVHPGGTFELFGGMSRDTVLEIDPYFIHYKEVD
                     LVGTFGFSEKDFKKAFGFITSDPDSFSKLISKVRDLENIVEAFNDATDKTKAKIVVKV
                     SS"
     gene            complement(11038..11535)
                     /locus_tag="PKL93_05700"
     CDS             complement(11038..11535)
                     /locus_tag="PKL93_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ70792.1"
                     /translation="MKKALLVVLAAMLLISAAVIADPVIKISGVIALRNEEGAWQLTL
                     DEFKELPQTEFFVTDPWMGDKTYVGVTLADLLKFVGVPSGAKNVVMVCSDEKEFAVAY
                     EDALKYPIMLTHSTRSGENLRALPASQGGPIKLAYPINDFPEITDKYPSDNWAWYVIE
                     VRVEM"
     gene            complement(11551..13152)
                     /locus_tag="PKL93_05705"
     CDS             complement(11551..13152)
                     /locus_tag="PKL93_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ70793.1"
                     /translation="MKKALLTVFLFLFLSAVFGIEGHVFVDLNSNGILDPEDQTVQGC
                     LISNGIEIVSSDSEGFYSISDSDGFIFLIKPDGYVCSEWYVKGLENHDFLLMPAVENG
                     IFAVVNDIHYADDPDLFYESLGDREMIDSPDEYMSKLVSILEIVKPDFIVCNGDMGAS
                     IKDIDDEIATRWATKVKDYLTISNIPVYYSVGNHETNKKKADPYDIFHNVYGPEYYSF
                     NSFGTHYIVLNTHNIIEGSFVYEIDSPQLEWLKKDIELVANNNRIVIFSHEPVFSLAK
                     TDNYYELMQIFADDCSYHITGHKHTMIEYFDAPFVELTCGAVSGAWWEGPSPTGDEYG
                     FTLFEMKRSDLNYCFVTLTYDRSGWFDMSRESPYSGVEYIRFCTFPSTEDIVVLLNDR
                     VVECDVLKKEMRFWSEYYFNVNLSSCDDGLNEIVVHSGDREFRKKLFVRSASVDIKSM
                     KTNPEYFEGSLVLVSNCANTGQWGKTYTFNDGSDTFMVQITNLDVPFTISKEYKYSLY
                     GIFRNSERVVDPIKLLVAEGIVQEE"
     gene            complement(13173..13910)
                     /locus_tag="PKL93_05710"
     CDS             complement(13173..13910)
                     /locus_tag="PKL93_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HNQ70794.1"
                     /translation="MTGKLIRMNRILREDGKTVIVAMDHGQFQGPIEGIRDVRRTLSN
                     IVAGRPDAIILNPGVIEKNGDLLGGKVSILCRITGASTNYSTKFDYHRITTSVEHALS
                     IGADGVVVMGFIGGDGENASLEIIGKVAEKCSKLGMPLIAEMLPQATDHFTDPEYIAL
                     GTRVAYELGADLVKVYYTGFESFTKVLESVPVPVVIAGGPKGKDAFEMAREALKLGAK
                     GVAYGRNVFQADDQTEYVEKLLKTVHG"
     gene            complement(14190..15008)
                     /locus_tag="PKL93_05715"
     CDS             complement(14190..15008)
                     /locus_tag="PKL93_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNQ70795.1"
                     /translation="MNRLLSLTIMVLFTSFLCASMPEQVLVNSGSFLMGTTWREEGVD
                     NEPVRRINLNYDYFIGKREVTFDEFDTFCEETGRNVPDDSGWGRDSRPAINVSWWDAI
                     AYCNWLSFKEGLPLAYDSEGHILDAMGRRTTDPSEVKGYRLPTDAEWEFAARGGNASE
                     GYTRLVGNSVDLVAWYKSNSENLTHPVGTKAPNELGIFDLLGNVWEWVSDYHTDYSCS
                     EEANPYVDSFSAYRVKRGGSWIDCPVGVSIDVRSKGLPDHAITNLGFRICRTAK"
     gene            complement(15365..15787)
                     /locus_tag="PKL93_05720"
     CDS             complement(15365..15787)
                     /locus_tag="PKL93_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNQ70796.1"
                     /translation="MRIGIIAYSKTGNTLYVAEKLRDKLTSAGKAASMVRLRSETPDL
                     ERYDGLIFCSPVNGGAPADAMKSYLAKMKSLEGKKVAVMATGLFPAALGRKQTLTYMK
                     KICREKGAEICGEGSVGWWSFGRERKIERLVEDLASCF"
     gene            complement(15793..16503)
                     /locus_tag="PKL93_05725"
     CDS             complement(15793..16503)
                     /locus_tag="PKL93_05725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNQ70797.1"
                     /translation="MTTIYLMRHGQSQANVERVFANGDDGFPLTDDGRIQAKMAARHL
                     KLRNIRKIYTSPILRARETSQIVSSDLGIKSQLLDEIREFHVGELEGQLIQGEAERAF
                     LELVKSWIDGRIDEKIPNGESHREVIDRFWKAMNTVVRECPEGEVLAVSHGGFLSMTL
                     PFVCNGIDPKSFFSRSGISITNCAITTVKAQRDNGSLSLELVDWANASHMELDFEKEP
                     IDNVWNVQSRGNTDQKGR"
     gene            complement(16723..17097)
                     /locus_tag="PKL93_05730"
     CDS             complement(16723..17097)
                     /locus_tag="PKL93_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70798.1"
                     /translation="MKKSFLTALSILAIIFLASGCLFFGLPNLNGSWDVVMTYSGGAS
                     KTSTFHIEQHIIYNYRGKFCLDSTCKELFGMISNDHRVNINTWTSESKIDFAGVAEKG
                     TMSGTFTRFEPSAEGTWEATRR"
     gene            17656..18465
                     /locus_tag="PKL93_05735"
     CDS             17656..18465
                     /locus_tag="PKL93_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HNQ70799.1"
                     /translation="MSGKKGKFVFNADFIYKMPVHFGGAPFYPVRVVYGDNTVICVDY
                     ETDENALLSYIPEDFELKEPVVSVQYTNCRDVDWMIGGEYRLIQVTTPVKYVGNSEGL
                     EGVYALVVWENKTCPIIGGREEDGVPKVFADISSERHLENHWFVAASYEQFTFLKIDL
                     QRGDEVSEEDLSELNKDSKINLFGWRYLPNLGKGGATLSHATLYPQEMVLRKMWIGTG
                     AVKWTGLSYEQHPLQTRIIRSLEALPIRRVKSASMAKGIVKLNVGDSRILP"
     gene            18485..19291
                     /locus_tag="PKL93_05740"
     CDS             18485..19291
                     /locus_tag="PKL93_05740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ70800.1"
                     /translation="MNEYFENKVAVVTGAASGIGLGLTEEMLSRGAKAVFMADVSQEN
                     LDRESERLSSKYEGEAVPFLTDVTKEEQVEKLIKSARKYEGHLDFVFNNAGIGMTIPT
                     EMVTLEIWKKVIDINLWGVIYGTYHAIPIMREQKSGHIINTASITGLVPVPYQALYAC
                     SKSAVKTMTESLYYELFNEGLKFSVVCPGNVRTAIFGGLTPPPDSVSVEEAVNYIVEK
                     MEKGDLLIVFPQKMRDFDELYRTDRGKFDEFAFNLAAERRENYRTKGTYY"
     gene            19307..20506
                     /locus_tag="PKL93_05745"
     CDS             19307..20506
                     /locus_tag="PKL93_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70801.1"
                     /translation="MNLTKVFDESELSLEVVILMIAGLILLITGILLFPVATGGLSYY
                     ENGLYGLLLVMFSLQIISMGKTPFGDLKRSKLVLLAGLVVGGIGTITCFVPDAFSDIP
                     RFMLFLFFGPGGALLLLQMILSKDKLKAWFEYGGIFKHLIAGCTVAYVSSILISILLW
                     NQSLLSVQMTAIFVLIYGAAIAYLSFVLRKIYSSYPQEQKRKDGEVELPIDRAMILFT
                     SVFMIILGVLLIPVNLGLLPFSGSAQLGLLMMIFAIQMIASGSTPIGVFPRSLPVILI
                     GFVFAALGTVSCIIPEVLVYPLTVLVGVLNILGGAISIGKFLGQRASSTDQQKGKIPK
                     ILVKLNVSQLILNVLAIAFGLSMLISHLLPGLVIGVVLAANGVVLLYLLHILLVIDRI
                     QKEMETR"
     gene            complement(20688..24896)
                     /locus_tag="PKL93_05750"
     CDS             complement(20688..24896)
                     /locus_tag="PKL93_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNQ70802.1"
                     /translation="MNRIGLCIGSSTISWYDGQTPRRLLHEGNPLKVLKSFLPEILET
                     GLVVVTGKKGRKLLNLPQIPEVEATEFAYKELKSKYGDHEAIVSVGGENFTLYKLNDK
                     GNITAVFTGSKCASGTGEFFLQQLKRMDIGLDDANAVETDEIYELSSRCTVFCKSDCT
                     HALNKGTPKESVLNGLGKVMSDKIGELMHRAGVKKILLVGGVTKNRLMLNHLEEIAEI
                     TIPEEALFFEAFGAYLHANTLEEIEIEKESVFKEGVMTFPKNEPLSKFVNMVDFKNMP
                     FKTAESGDSCILGVDVGSTTTKAVFMRARDNAILASSYLRTMGDPINATRQCLDEICN
                     SLDVPIEIIGLGVTGSGRKIVGLYCETSAVYNEIMAHARAASFFDREVDTIFEIGGQD
                     AKYTFLSNGVALDYAMNEACSAGTGSFLEESAKESLNIDYREIGEYALKGQSPPNFSD
                     QCAAFISSDIKTAINEGISREDICAGLVYSICLNYTNRVKAMRPVGKKVFMQGGVCLN
                     QAVPVAMAAVSGKEIIVPPHPGLMGAFGVSLMVKEKLRAGQLEEERFELEALISKEVR
                     YLNTFICDGGAEKCDRKCPIRIIEVGGKKFPFGGACNKYENVRLNLPINRNEYIYTTE
                     REKIVFKLPEEKGLETIGISKSLSMNTLFPLFSNFFSSLGFRIVVPDKSDPRGWDRKK
                     SEFCFPVELSHGYTYDLLDKNPDYFFIPAVKGLEVENSVENSVFCPFVQSEPDWLMGS
                     FEELRSKRILTDFFDFSQGFEKEKEKFVSLAKSMGRNREEALAAFSNGVKSYEDSVRR
                     IKQIGERFLENLKNSDFGVVIFGRAYNAFSSDANMGIPEKLASRGIPVVTFDALPYEG
                     ERSYEKMYWAWGEMNLKAARYVRNNPKLFALYITNFSCGPDSFLISFFRDIMGKKPSL
                     ILELDSHTSDAGVETRIEAFADVVRSSLERKTDLGEKKRAKRPKLLPNNGTFSIELQD
                     GRHIPITDPSVKVIVPTMGEFAAQLIAAAFEKVGINTHVLPRPTELEFQLGKSNSLSK
                     ECLPFHLTLGSLIRYLEENKDDESILMYFMPDTRGPCRFGQYSTYIDLWLDKNNANNV
                     LLFSLNSENGYAGIGLKAKLRIWASMIIADEFCNVENALLTLASDRDEAAKATKEARK
                     IMLESVKRDSFTEMTKKLEEICESISHIPLKGDFESAPKVLLSGEIYVRNDEFSRKSL
                     ELLFADNGIIMHASPIEEWIYYLDYILLKKYVPTELGLLKRGTKHVETLVKQSFEKRI
                     KKILEKSGLYKSHFVDIESMVNTSKDFLNPKLTGEAILTLGAVLFESIDTYDGVISIG
                     PFGCMPTRIAEAIAERGLEHLRENSSKLKREIFKNAGNIPILFFESDGNPFTPTVESR
                     LESFVVQVKRVKSLSMKLKSREFK"
     gene            complement(25180..26583)
                     /locus_tag="PKL93_05755"
     CDS             complement(25180..26583)
                     /locus_tag="PKL93_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HNQ70803.1"
                     /translation="MGMKNSLALISAILVFAVIIGAESTSVTSNYEEAREVARQAYIF
                     AFPMLENYRTMVLQAVIPNSFNEFEHLQGLLGPEFREIARPNNDTLFSAAWLDLRNEP
                     VIIEIPSICERYFSVQFIDMYTHNFAYAGTRTTGCRKLTVMVSGPSSFVEIPNEIDEV
                     FTSEGNFVFCIVRISVNPEMPGDIDAVMKIQNNFKIEPFGAFYGSQTSSSNKSIAFPH
                     FSQEKAESAGFISYLNFLLGQLEIHPSEKALIERFGLIGIGPNLPFNENDLQPEMITA
                     IEKGIEDAMSTIFRPLEKLGVTKNGWNLTKRIFGSRQQMQGKYEIRAAAAYVGLYGND
                     LEEAYYPISYVDADNESYDGSKYDYKIHFESHEIPPVEPGGFWSITMYGDDQFMVPNQ
                     IDRYSIGDRSRLSYNDDGSLDIYIQHDSPGPDLERNWLPAPNGPFSLSLRMYLPSPRA
                     LDPLYCPPGVEKERPRD"
     gene            27004..27753
                     /locus_tag="PKL93_05760"
     CDS             27004..27753
                     /locus_tag="PKL93_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNQ70804.1"
                     /translation="MNAKQVAELSDLSVRALHHYDEIGLLRPRRNAENGYREYSEEDL
                     DKLQQILFFEQCGFSLERIKKLPSNPRFDRVKTFELQKKHLILEKERIEAMLSTLERT
                     LKTLKGEDSMSQREKFKGFDFSYNPYEEEAKQLWGDETVEKSNDFITSKSDRERKDME
                     KILDDLFKGLAEIRIKDPDSDLAQEAIDRMYRVFNDSFGHYYIFESFAALDSICVMDD
                     RFKANTDKYGEGLAEFLSKAMREYADRESEG"
     gene            28137..28985
                     /gene="pheA"
                     /locus_tag="PKL93_05765"
     CDS             28137..28985
                     /gene="pheA"
                     /locus_tag="PKL93_05765"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNQ70805.1"
                     /translation="MKRSIAFQGEHGAYSEQAIRKLFGESPTTFPCRSFREMLKLVSE
                     EKVDYAMLPVENSLAGTVIPAYDALIESELFVHAEVMLRIEHCLMAPEGVKIEDIRYV
                     ISHHQALSQCMRHIEEEGLEAKEYYDTAGAARDLGTLKMPFTAAIASELAAKTYGLEI
                     LRRGFEDLDTNTTRFFLMGREARKCEGRCKTSMIFTTEHKPGALFKVLGELSDRNLNL
                     TKIESRPFTKEMWHYLFFVDFEGSVSEKRVEEAVNAISSRCSYFKFLGSYRSANDVEL
                     NSNQLT"
     gene            complement(29200..31143)
                     /locus_tag="PKL93_05770"
     CDS             complement(29200..31143)
                     /locus_tag="PKL93_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNQ70806.1"
                     /translation="MIDVELMELPLGEIRPAGWLERQLKIQAKGLTGRLEEVWKDVGP
                     DSGWLGGSGENWERGPYYCDGLIPLAYLLEDEKLKGKAAKWISWTLESQNEEGFFGPR
                     DNDDWWPRMVMLKVLKNYFEYTKDQRVVHFMTRYFMYQLQNIDSREFAIWENTRSVEN
                     IYVILWLYSITGEGFLIDLAKRFFEKSMNWSNYFENFKYTKPTGEYLDWEKFMKATGG
                     KGLEGLYEYEERTNSTTYSKLFQETHVVNVVMGVKYPALRYLLYGEKNQLKIVKEGIK
                     KLIDYHGTSNGMVTGDEHLNGNDPSRGTELCAVVEYMFSLEILFRVFNDTEFADILEK
                     IAYNALPATIDKELLTHQYDQQVNQIMCSRAKRNWYNNLDDSNLFGLEPNFGCCTANM
                     HQGWPKFVKNLCYKSDDGGLVFGVYGPCKIETSVAGQTILIREVTDYPFDDKIEILID
                     NNADFEIPLHFRIPGWATKSEVTLNGSAFDPENNEGYLTIKRLWNRGDRVELVFRSRV
                     RTPKWFKNSLSVEKGPLLFALRLDEKWNTTNKDGEFPEYEVYTTSPWNYALKVDLKNP
                     EKSFSFIKRTQQLSEQPFDQNRVPVLIEADAAKVKEWSWERNSAGTLPESPVRPEGPL
                     EKVCLIPYGAARLRIAQFPYFEE"
     gene            complement(31176..31997)
                     /locus_tag="PKL93_05775"
     CDS             complement(31176..31997)
                     /locus_tag="PKL93_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ70807.1"
                     /translation="MSKKLFITLIIISIIFVIPIYWAAITSFKPSSEVLSYPPSLVPK
                     QPTLAQFTKLFTAGDSIFTRYVLNTFILCGLSIGMVLILSIMGGYSLSKLAFKGSNII
                     FVIILSIMMVPYQSLLIPLYDLMNAMGLLDSLFGVSLIYTTYSMPFCIFMMKNYFSSL
                     PNTLRESALIDGASEMRILFKVHLPLALPAIATVIVYVFLMTWNDFILALNFTSSNDV
                     RNIQVGITLFATTRFTRDWGLINAGATFSIIPSILVFLLLQKYYVEGMTAGTNKE"
     gene            complement(31990..32865)
                     /locus_tag="PKL93_05780"
     CDS             complement(31990..32865)
                     /locus_tag="PKL93_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70808.1"
                     /translation="MKQKTRKGLFGFIFLLPVIVMISIFFIYPLIKVVLMSFQEWKIM
                     GGSSFVGLSNYKKALVDDEFWKTLWNTVIYAIIVTPMIFVPAVLLANALKKTSRSTKI
                     FRTIFFIPYAISFVAASYIWQWIYNDSYGILNYILISLNLIGQPINWLGQTWLSRVMV
                     SIMVAWKTLGFTMIIVIAGLQGISPQIYEAASIDGAEKSQVFRYITLPLLRPTLVLAL
                     ILSLAGSFKAFDHFYIMTKGGPLKTTETIVMYINKIGFEFYDIGFGSAVSVIFLGILL
                     LISYLQLKAGGFQNE"
     gene            complement(32927..34174)
                     /locus_tag="PKL93_05785"
     CDS             complement(32927..34174)
                     /locus_tag="PKL93_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70809.1"
                     /translation="MSRRLLVLILVVTLAFSGILMAKTTLVMWDQLAATEDIVKMFNE
                     KMKAEGKDIEVKLELIPYDQMVPKFMAALSAGIAPDLYGMDLVQFPFFISIGAFTDIT
                     EWAKELPFFDELTKGMLEIGMKDDRIYGLPYQIDLSTMLWNKGLFEEAGLDPEQPPQT
                     WYELVEMGQKLTVDKDGDGIIDQWGFNLAGGGAGAYMFWFMPFVWGNGGRMFDDEGNV
                     ALDSYETVQALQFWADLVHKHKIAPVSSVQYGSGDRYNAFVSGKLAMFLGGNFNITSL
                     LQDAPDMEFGVAMIPKNRGEFASFGGGSLLGITSQSKNVEAAKEFMEFAFSEEAQVGA
                     FAPSLQLVARPSLYDNEYYKDIPQMMRFADILGIAQTPYTVKYNEIYDPVGYYFESVF
                     LGQMDAETAVRECTKEIEKILAE"
     gene            34426..35418
                     /locus_tag="PKL93_05790"
     CDS             34426..35418
                     /locus_tag="PKL93_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ70810.1"
                     /translation="MKKNYATIKMIAEDLNLSTSTVSRVINKHPDVSQETRRRVLKKI
                     EELGYVRNATATLMRNNVSTTIGVIFEAEYDPFFSEILQGIEKGISKYNYRMILINTK
                     LRSAEARSAVNMLLEHRVGGIIIVPSNPEISYAKDLVKRQFPVVVIGRDYKELDVDEV
                     FTDDYHGGELAGEYLVSRGATRLLMINSRDSGSASSEREKGFVKIAEDKKVYYEVIND
                     RVTDYDNTFKIVRSIFSESIPFDGIFCFNDTRAFAAINALRSIDESLLRKISVIGYDD
                     IIFSSIFSPKLTSIRIDKDMEGFEAVKLLDQRIRGVRKSVKREVLNVSLVIRET"
     gene            complement(35641..36363)
                     /locus_tag="PKL93_05795"
     CDS             complement(35641..36363)
                     /locus_tag="PKL93_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70811.1"
                     /translation="MKKIAVLSILMLILGVVATASPGFRNDMDGEILELRLQIQIANA
                     INAANLSPEQLGQVYDLAKSTRDELEALKERVTNTLEETLEKAISGEEATFPEIDSTR
                     TRMQSIVENYLSGLKSVITVEQAENLAEYFRERPLANRSEVHEKNAPIIGRIEERLPE
                     IEEQMRQQFRNLPPEAQERLKNLDVDERTRVMKEKMTDRLNASRPGIVERPVQNKFTT
                     QRLAMVLLSDEALEVMEKMLGQ"
     gene            36671..37204
                     /locus_tag="PKL93_05800"
     CDS             36671..37204
                     /locus_tag="PKL93_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70812.1"
                     /translation="MNDYLSRLYNDLVNNTREEYRMKDYDKYFTVSSKSRKITPNEEA
                     MREAARNYGYFALLSNEVNDPFEALSLYRSKDIVEKGFGNLKDRLNFRRMQVSSELSL
                     NGKLFVEFVALIYLSYIKKKMQDTGLFENWTLQDLLDELDTIERFESPEHGRLIGEAT
                     KKQKDIYVKLGVKSPSL"
     gene            complement(37556..37828)
                     /locus_tag="PKL93_05805"
     CDS             complement(37556..37828)
                     /locus_tag="PKL93_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70813.1"
                     /translation="MRVKVKEKYASRVVSSGRRSFFEFDEYWGSQLLSNDFGIRHEDC
                     ERNLYGRDRAASLSSSQTNLPSSGEIFETGSSESLSRKTFLTISAF"
     gene            38217..39134
                     /gene="dapA"
                     /locus_tag="PKL93_05810"
     CDS             38217..39134
                     /gene="dapA"
                     /locus_tag="PKL93_05810"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNQ70814.1"
                     /translation="MDVVGKFGRILIPMSTAFAADYSIDYPMTRRIASYLISKNYCDS
                     LVIAGTNGEFYSMSFEEKVRLFAEVKQEVGERVPLIAGTGTANTHETIELTKEAARLG
                     YDAAMVVVPYYCHPTQEGIYYHFSQICKNTDLPIMVYNIPLFTASNIDPSTLAKLTEF
                     ENIVAVKDEAGINPLQTSAFLKATEGKIVVYSGDDLMVLSVMSQGGIGVISGGSHVIG
                     DMMRNLIEEFLSGDATKATGSFKELYSLFSAFFGRNRRLVNPLPAVKKAFALHSGLDV
                     ARVRPPLMEIEEDEVAVLKDTLRKFGKIS"
     gene            39170..40171
                     /locus_tag="PKL93_05815"
     CDS             39170..40171
                     /locus_tag="PKL93_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNQ70815.1"
                     /translation="MKKAFILVFLLLTVFVCFAVEETYTIRFNTVAAPTQPQVLAMNK
                     FAEVVENLSGGKIVVKVFHSGQLGDQQTSLLAVMRGDLEMAGDGAPSWFADLGNMPKM
                     GVLNAAYIFKSLDHMYSVMNSPMVKGWFDELASNTGMRVLDTWYLGMRQLNTTEKAGP
                     VYKPEDLKGMKIRMPNNEAFMDMGRAIGSNPTPMGFGEVYLALKLGTIDGQDNPLPTD
                     LSAMFVEVTKYIILTDHSIGMINPVINEKFWQSLPFEYKTYIIKALEVARYYMNYLVL
                     EQESKLLKQFVDEYNMEIIVPDKEAFMNLARDFYSDEKFDKAWGEGMYETIQSYPLN"
     gene            40237..40767
                     /locus_tag="PKL93_05820"
     CDS             40237..40767
                     /locus_tag="PKL93_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNQ70816.1"
                     /translation="MKKIFLSLLKTLQILLNLIESWGGTIFLGLLFLSIFFQVILRYV
                     FSSPSPELFEIAQYSFIWTIFLGAPYARKFDAHIKFDILFLRFSRKVQLILQIVFDLF
                     FCIVLLVSLGPVLGDVIFYKIIKSEVLRIPWTYLFMCFPIFMVLTFIHNVIWIVKSFL
                     ELISGKTFAKEVEPWD"
     gene            40758..42053
                     /locus_tag="PKL93_05825"
     CDS             40758..42053
                     /locus_tag="PKL93_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNQ70817.1"
                     /translation="MGLILFFIIFGATFALGYPIAFGMIMGGFAYFITSGMDLGIFLD
                     IMAIELRAQDVLLAVPMFIFAANVMNDTEITDRLFGFLKQLLGGVRGGLAYANIGASI
                     IFAGMSGSEIADVSGLGTIEIKAMMDDGYDGPFSCAVTCASATISPLIPPSIPMVIYA
                     MLTGTSLGYLFLAGIVPGLFLGFIEAGMALYLSKKRNYPKGQRYPLKVIAKSFIRSFP
                     ALLAPVILLAGIYGGIFTPTESAAVLAAYSILVSIFIYRTLGLKKLRKIMLKTVYNVG
                     AISFMIAGAFVVKYVLAREEIPLLLTNAFVDMGLLSSAWVLLLSVNVLFFILGMFFDV
                     SVIQLVIIPIVFPLVRAAGIDPVHFGIVTTFNLMLALDTPPYGQTGFITSAISKTPVG
                     KVFKEMLKYWIPIEVMGLVIITYWSDFVLWLPRLLGYAH"
     gene            42080..42835
                     /locus_tag="PKL93_05830"
     CDS             42080..42835
                     /locus_tag="PKL93_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNQ70818.1"
                     /translation="MQSVERIVKILSSFSLEESRLSLDDLTKKSGLPKATVYRIAEAL
                     CKEHVLEKDSQTPTYRIGIKLFELGSLYLSSMKLKDVAFAEMEKLHLQTGETIHMGIL
                     DGTEVVSIEGFESFKSLHTKLYIGKRAPLYCTAVGKAILAFLPEADREGLIDTLDLIP
                     QTSKTITDKAKLREELERIRSRGTAFDKGENEDGIACVGAPIFDSSGVVVASISISGP
                     IFRMEEEVMKKYSDLLKKVVSRISSALGHGLQQ"
     gene            complement(42857..43524)
                     /locus_tag="PKL93_05835"
                     /pseudo
     CDS             complement(42857..43524)
                     /locus_tag="PKL93_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744876.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            44125..>44789
                     /locus_tag="PKL93_05840"
     CDS             44125..>44789
                     /locus_tag="PKL93_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70819.1"
                     /translation="MRTYRNRKLRFRKLMIFVAAISFIFLSTECIFKTVTLTMLEPDE
                     GGEVQPLPGNHKYLINTSVDIQAMPDDGWDFDRWEVDEEFYSNEKETKLEMDSEKTIK
                     AFFSRQPVTLTMEDEEGEGSISPVPGEYQFDYSTLVGLSATPTEGWEFDRWQVDGVFY
                     SSEEETELSMDADKIVEAFFNEEPVASVTLSMHEPIGQGAVAPTVGDHKYDAGKSLTL
                     QATP"
BASE COUNT        12114 a        10085 c         9786 g        12804 t
ORIGIN      
        1 tatcctatct gttcttctat ttatctccaa tatccttgcg gggtaaatat gtttttttcg
       61 gtgaacggag aaccgctcaa cggtcaacca gtttctcttg atttcccagc ccccgacgcc
      121 ggtcttacaa ctcacaagtt gcatcttgga actaatcttg gttttcacat tctcttttcg
      181 gctatcgaaa catgagtcaa ggtaactgga cattcctctg cactaagaac tacttcccag
      241 ttctttcctc cgtcttttgt gcgcagtatc tttccgaatt gtgggtatcc ggccgatgag
      301 ccgacaacca acgcgttact cctgtcgaga gcatctattc ctaacagaaa ggaattgctc
      361 gttcctgccg gaggctgctc ctcccaactt attccactgt tttcagtaag gattatcgta
      421 tcgaaatcgc aggctgccca gtaagttgaa ctatccagca ttaccagaga gttcaggtct
      481 ttagcgaaaa gaggacctcc cgtaacccat tgtttcccac cattcgcggt taccacataa
      541 tggccctggc caccgtggat aactatgtgg ttttcatctg tggcctttac acctatccat
      601 ccattgtcat tgtaattgtt tggcagttca atctcttccc aggtctggcc accgtcttcg
      661 ctcttcagaa ctatgccggc tctgggtgtt gatttgtttc caacagcata aatcacgtcc
      721 tcgtttattg catggattcc ttgaataata tactctgaag ccatttctgg aaggtcacat
      781 actgtccagc ttccaccgtc gtcatcagag aagattacaa gtccttcgtc accgcttacc
      841 caaattctat cttcaaaaat cgaaaggaaa gagaaaaatg aatcagtagc aacttccgat
      901 actccaacta tctcccagtc ggaaccgccg ttagtcgttt tcagtaataa cccgtctgaa
      961 ccgcaagccc agactgtatt ctcatctacg gaaagaacgt cctccaaact cttaccttca
     1021 ggcaggatgt ccatggcctg gcgtgtccag gtttcaccgg aatcgtccga gaagtaaagc
     1081 attgcgattc cgtttgtgtc tgtctgcccc actacccatg cgcggtaatt ctttactttg
     1141 ggaaaaggga tgcagctgct cacaagaaat agaatcacca gaaaacctac aaatgctatc
     1201 agtatcttcc tcttcatttt ctcctccttt gaattcactc tacaagagta atggtcgcca
     1261 ctaaatgtta tcgtgggaat attagaaagc tgtagtccgc atttgacgga acggatttag
     1321 agcatctcgt tcatatcgtc tctagtaact aagagctata tgaatgacga cagtagaata
     1381 atagcacttt caccttcttg aagatgagtg ttatagagtt agtatccttg gaggagggtt
     1441 cattctcgca gccccatgct tcatgaaagt cgtgaagtct gtcggctgtg aattaatttt
     1501 tggggtagtc atgtcaagta gattcaggat tcgagcaggg gtttgcagcc cagtcagctt
     1561 tatgtatcgt ctaccaaact cagagctatt cagagtgaag agtgtttcga ttccggtcat
     1621 ttatattgat gaaagacttg ctataaccga cgggcataca agagccgttg cggcaatcat
     1681 cgaaggtcag gattcaatgc tcgtcgagat aacagatgag aagctggacg gcatatgagg
     1741 aaggtttgaa gtggtgctta gaggaaggga tttttacgcc atatgacctt gttggcagaa
     1801 caattatcca cgaggattac agaatgttct ggtacgaaag atgcgaacgt cttcacgaat
     1861 agttgaagtc taagcgaagt agtacgtagt gaatatccta tcggccgatc agtttagttt
     1921 tatcgctagg tctgtttcta ggtcttgcct gccttttgct cgtttagtgg tccttgataa
     1981 ctaagcgtgc ttattacgaa gaatagtatt gcggccgcga agcctcctgc aacaaaaaca
     2041 aatgccagat tatagccacc tagggctatc aaagaaccta taagactcaa ggcaagcgat
     2101 tgagagagca ccattactat gttatttagg ccgaagatct ttccctgatt tgcaagggaa
     2161 accgtatctg agactattgt cacgttgagg gcgagaacga ttcctaaagc aaagctgaag
     2221 agaatcaaca cgatcagcat cagaaatggg ccaagatcca ggaatccggt gtagaagaca
     2281 ctcaccaaca agaaaacgcc ggctagaata gaaagcgccg taatgtgcag ccttctctgt
     2341 cctagcattt tgcttagagt gccagtcata agagaagtca cgccactcac aagagttgat
     2401 attcccatta ttgacgatgc agtgccaaga gacacaccaa tatcgctgct gatgaaagag
     2461 gtcaagtaag gaagtatccc acccatggaa aaccataagc caaactgaga gatcatcaca
     2521 aagactatct ttctgtcaat tctgaatctc aagtcttctt ccctaatacc tacagcggca
     2581 tcatctactt gagaaccaga acgaaagaag aaaatcggaa taactaggac agcagcagaa
     2641 atcgagaaaa tcacaaatgc ggtctcttcg cttcttgaaa gggcgactcc aatgaacagg
     2701 gaacagagaa aggtcgctat attcataaca cccatcatga atccgtagtt tctgtccttt
     2761 ctgccgggct tactcacgtt gctgacaagg gcagagtatg gagtaagaag actgaatatt
     2821 gacatggtga aaacgacagc agcaataccc aacaagattt cattgcctgt gctaagcatg
     2881 aacatgctgg caattgcgat gatgaagaga cctttaatga agaaaaggta tttcctgttt
     2941 cggtcgccta gatagccaat tagcattccg gtgaagatcg ttataatcat tcctgcagaa
     3001 accaggtttc ctgccagagc ggttgagtga aacttgccat tgagatacgg gattagaacg
     3061 aaattgaaga ttccatagat gatccccaca gaggcacttc cggacaatag tctaatcatc
     3121 tatatcactg gtctcctcta cgtatcgcac atacttctcc agatcaagat tttcgtttgt
     3181 tttcaatact ctttgagtta tgaagtgagg gatattgtta gtttcgcctt ggagagaaga
     3241 cagtgaacct gctattgctg caatggtatc ggtgtctccg ccgatcgagg cacccattct
     3301 tattgcttgc cacacatcat ctctgcagaa aaggccgatg gcaatcgctg caactgctac
     3361 gtctaccgcc tccatagtcg tgccaaacag atagtagatt ctgtcgagaa aatggtcagg
     3421 caattcgccg cctatgagat ctctgatcaa agaaagtctt tcaccggcag aaggtcctac
     3481 gaattcggcg caagagagtt cttcagctaa tcttgagcct ctaatggctg cttcgataat
     3541 cccattgaca ccctgcccct ttgctgcata gtagtaggcg aatccgactg cgagggaagc
     3601 ttcgagagca agattcgtgt tatgagtagg gaccgtgcat tctattatta cttcaattag
     3661 atgcttttca cttttgttga ggctgcataa gactggagca agcactctca ttggggcccc
     3721 actggtagtg cccagactct cgaactcttc tttgttcttg aaggcttcga tagctctttt
     3781 agtgcttggg cctatgtatc ccttcgcaat agggtcacgc tcgtcggccc atctgcaaag
     3841 agcttccttg accgtctcct gtaagaagtt tcttttctca ttgtagagct ttatcaagta
     3901 tagcacctgc tctgtgtcgt cagttatctg acctcttgtt aggtttttgt ggataggtga
     3961 gtgttccggt tctagcaaat cacttacgaa gtcgaacctg gcagagattt gccggcgggt
     4021 catgaattcc gttaccattc ccatcgcatc gccaacggcg aatgcttcaa gtgccctgaa
     4081 aattctgttc attcttcatc acgtcttttc acgccaagtc ctgcccttcc atttattttc
     4141 actttgattc ccttggatgc tgaaagatcc acccttggat ttccatcgat tacgcagaaa
     4201 gcttcgaagc cggacaaact tactgtatca ctgttagtta gaatgaactg cttgatctcc
     4261 gcagagactg tctcgcccgg ttcaaccata gttattatcg gtctggaaga gacaatcatt
     4321 gccgagttac aactcgagta aggcagccag caaaggaggc cgcttactgc cttataggcg
     4381 aacaatccct tagtgtcttt ggaaaatgga gagataatga tttgagatgt acgaaaatga
     4441 ggtgaaacct cctctccgtt aatctccaat ctgcttgttt ctgaagcgat tgatacaggt
     4501 tctctcggtg ttctctcatt cctgtagaag aagttcgaag cgtcgattgt taagttcttt
     4561 ccttctactg tcgaaatcaa agtttcggaa atgactacat cgttgaatgc cgaacttatg
     4621 tagtcctctt cgaagacatc cagtcccgtt acccatttag gctctatatt caggaagtca
     4681 aagtcaagta tgtcagcggc cgatctgaag acgatcagtg gtccgcaatt ggccgttcct
     4741 aaagccacac ccgcaactgg cgcaagcctt ccagtcagcc gcataccggc gacaacatcg
     4801 gatgcagttc catcccctcc gatcgatatc accagattca tttttgaaag ggcgttaccg
     4861 gcgtttatgg tgtctctgta atcgttgtgg atcgtccccg atccggttac ttctagattg
     4921 aaatactctt tcaggtgaag atgggtagat ttcgtgcaga ataggtgcaa tttctcaaac
     4981 ttcttggcaa gagccttcat gagatcaagg tctttctctc gcttaccaga gtttggattg
     5041 tagactaatc cgattctcat attaactctg cattcggagt atttgaagag cagtactcct
     5101 atccgttacg attgaattga tatacccggc ctttattgac gcataaattg cctgagcttt
     5161 ctccttgcca cctgcaatcc caatgacctc tggtacttcc ttcagctgat caagagatat
     5221 cccgaggata ttctcatttc cggaaacgca aggcgttccg tccgcgttga agaatctcgc
     5281 tgcaatgtcg ccaactgccg catgtttcat aaggttggca aggaactcct tgtcatagat
     5341 gttcttgaag gcgttggaca gggatatagg ctcccctatc ccgacaatgg ccagatctaa
     5401 cctcttccac atctccgaca tgaatcttat agaactgtct tcaagcatca tgtttagagt
     5461 cttcgcgttt tctatgaagg ctggagcgaa aaggtagtaa cggttactgt gaaaggaatt
     5521 cgcaatcttt tcgacaattg aattcatttg atactcgtga cgcaactggc cgacaccgcc
     5581 gattaggggt atgaatgttg tgtttggaag ctccccattg aatacgactc tttcaactgt
     5641 ctcgtacaga gttcgacccc atgagatccc cacaagctgt ccattcttca cgtaatcggg
     5701 gaggaatttc gctgcagaca tcgcaattga attgatagtt cctccatccg atgcttcgtc
     5761 cgcaacgatg acgttcttca gtccgaatct ctctttcatt tctattgaaa tctcttcgag
     5821 attttccact tttcccgaat caatctctat cctaacgact ccctcattct tggcttttgt
     5881 gagcagcctg ctgacttggg gcctggatat tccaaccaat ctggcgatct cgctctgtgt
     5941 cttgtcctca atatagtaga gtttgcaaac tttcatcatt gtgtctctat cagccataag
     6001 ccccttcatc ttaaatcttt cctcccttct acgaggttgg gtccaattac agaaaccgct
     6061 tctgccgcct tcttgtggcc gtaatagata ctctggattg gatcaccgca cttcgtgaac
     6121 ttctccataa atcctgcggc gaaagcatca ccggctcccg tagtatccaa gatttcttca
     6181 gcgattcctt caacctcgaa ggagcggcta ttcttgtttc tgtccatcca gatcgttgca
     6241 gccgatcctc tggttattat aatattcgaa gagatctcac tgatccttgt ctgaaggttc
     6301 tctgcttctc cttccgacaa aatagtgtaa tccgataaac aagctacctc aacaacggta
     6361 tcggctccaa aatacagacc acatccacct ggtacataga ctttcagaga ttcagctgtc
     6421 tttcggaatg catcgagcgc cacttctttg aaactgtccg ccacgcaaat cgcattgtat
     6481 ctcttttcgt cgattacacc acgaaagaga gcatggcctc caagggatat gattcttctc
     6541 tctccatgtg aatctatcat tactaaagtc tcagcgccag atccattttc cacgacgtaa
     6601 ctaagattca tgcttgacaa agagagcttc tctagaatcc acttcgaaga taaatcattt
     6661 cccgttccga agtagaaatc agtttcaacg cgacgcgaga gattcaaagc tatgtttgct
     6721 cccgagcctc cgatgaaatc atgtgtgctt cttgcaaata ccatagaatc tctttcagga
     6781 aatgaatcga gaatcacata cctgtcgata gaggcggctc cgatgacagc gattctcatt
     6841 ttatcgcacc tctcgtaagc cctccgataa gatacttctg tgatagtaga aacagaagga
     6901 ttggaggaag agcagtgatg agagcgccgg ccgacagagc gccccactct acggcacctc
     6961 cagttccaac aaaccttgaa aggaaaacgg gaagggtaca atttgcattc tctctcatta
     7021 ttacgagcgc aaaaaagaac tcattccaag agagaaggaa cgcgaacgtt ccaacggcga
     7081 ctatccccgg aactgccatt ggcacgatta tcttcctcat tgcctgaaat cgattacaac
     7141 catctatcat tgcagcttct tcaagcgcct ggggaatctc atcgaagtat cccttaagca
     7201 accagctgca gaacggaatg gtaaaggtag tgtatagaat aatcaagccc agggggttgt
     7261 tttgaaggga ataccggttc aggagagaaa aaagaccgat aatcatgaga attcccggga
     7321 aagcctggga agccagaagc catagtatca aaggattctt gcccttgaac ttgaatctgg
     7381 acattgcata ccctgccagt gtagtgataa ttatgcagag tagaacggtg acgagggtaa
     7441 ccgtcatact gtttgtgaag ttcctaccga aactggtgtc ctggaagatc ttcgagtagt
     7501 ttcccagata gaatttctcc ggaagcttca aaggattctg ataaatgtca gtagtttctc
     7561 tgaaagaatt tagtattagc attaagagag gagagagaat tagcagaacc actaaaaacg
     7621 ccacgatgtt cattgttatt actacgcttc tcttaggcct catttctaac ctcccttata
     7681 tagaaggcaa caggtagaag gagtataagc aaacatatga cgccgattgc agaagcgtat
     7741 ccctgctgga agtatgcgaa agcattttga taagaataca gcgatagtag cattgaagag
     7801 ttccccggtc cgccgttggt cattgagtag actatgtcga acatgttgaa agtccagatc
     7861 gtgttcagtg taacaaggat aagaatcaga cttttcatta aaggcagagt gagatggaag
     7921 aacctcttga atactccggc accatctatc tcggcagcct cgtatacatc agaaggtagg
     7981 gactgcaatc cagccagaag agtcacaata acgaacggga ggcctatcca gatgtttgcg
     8041 actatgcagg cgaagagaga ataacccggg gtcaccaacc aattgggagg atttgcaaag
     8101 cccactgcat tcagaaagga attaagcggt cccataactc cgaagatgta tctccagata
     8161 agtgccgcta caacgggggt tgtcgcccag ggcagaagga tcagcgctct gtatagacct
     8221 cttagcctta tgtatggtag attgagcagc agagctatca acatacccag aacaagctca
     8281 aatccgacac acgcagccga ccatataagg ttattcttga gcccggtcca aaacagattg
     8341 tcagacacga gatctttgaa attatcaagg ccggagaagt actttcctcc tccgaagaca
     8401 gcgtcggaaa agaagctgtt gtatatcccg ttgacaaatg ggtatacgct tattagcgct
     8461 actactacca gtactggcag tatatatggc attgccttcg ccgtttcgct ctttctgatt
     8521 tgtcatcact cccggcagtt accgagtgaa tcggtagaat gtatctagta gtatgccatt
     8581 ttcgcatttt actagcgaac acacgctgct aatacgaaaa ccacgaagaa acgtctcaga
     8641 aaaactcagt ctgaagtgct gcgagcttat gtagtgtatt cctttcattc tctacaagtt
     8701 gcagcaaatc attgaagccc gagtttcttc agcattttgt ttccctcagt aactgtatcc
     8761 ttcagggcgg cgtccagact catgttaccc agtataactt tttcgagatt aggtacaaat
     8821 agcttccact cgaaggagta gaatcctggc attcctggtt tcacgttgtc cgagtattgg
     8881 agccaggcct tagcaacatc ccattcattt tctggagagc tgaatcttgg atcttcaagc
     8941 gaagactctc tgacagggaa gagataatca ctgtagctta tctctgctga gttttcagta
     9001 tttgtcatga attctatgaa tgcaaaggct tcatccttgt gtttggacag cttcgatata
     9061 ccaaaagcct gggggtcaga tgcattgatc ctgttgtctt cattcttgac aagaagcata
     9121 ccccagttga aacctttgtt ctgaagcaag aagcgtctgt tccagcagcc aaaggtgacc
     9181 attgcgtact tgccgtttat gaacccctgc ataagagagg tgtagtctat gcttatgacg
     9241 tctctaggca tcacctggtg ttcagttatt agtgaaaggt aaaactcaat tgcctctata
     9301 gctccctcat tgatctcaac cttccattta tgattgctgt cctttgtaag tatcgttcca
     9361 tctttctgag cgatcaaggt ccagtgaaac ctttccataa gaggagcaag gattccccag
     9421 gttattacat tttcgttctc atctttcttt gtaactcttt gagcaatatc aagcagctct
     9481 tcccacgaaa tcattctgtc gagaggcaac ctgattccgc tttcttcgaa tatgtctttg
     9541 ttgtaaaaga tcacctgtgt ctcctggcac caagggatcc cgtataaatt tccggcaaaa
     9601 tcgtagcagt cttcgaatat atgagcagga atgtcgccaa ttatttcgtc actcacgtaa
     9661 ctgttaaggg gggccgcata tcccatcatt ccatattctc ggaatttctc ggcgtcagtg
     9721 tgaaagacat ccggcgcgtt tccgccttga aaacttgtca gcagatactg gtctgaagac
     9781 tcccaggtgc cccagataat gttcacctga acatctggat tatccatgtt ccatatatcg
     9841 accagatcgt atatcttgtc aacggctgca ggctgccagg caagacagta aaagtccagg
     9901 gtaatggcga agaccgccgc agtaattatt agaagtaatg aaatgacaaa taccttcttc
     9961 actcaaatcc cacctagctt gagaccttaa cgacaatctt ggctttggtc ttgtctgtag
    10021 catcattgaa agcttctacg atattctcga ggtcccgtac tttggaaatc agtttagaga
    10081 aagaatctgg atcactggtg atgaagccga aggccttctt gaaatccttc tcggagaaac
    10141 caaacgtgcc gacgaggtct acctctttat agtgaataaa gtaaggatct atctccagga
    10201 ctgtgtctct cgacattcca ccaaacaact caaatgttcc acctggatgg acgaaaggca
    10261 gaagcgagtt gacaagttcc tttctatcat ttgcaaggat gattgaatcg aaacgaggat
    10321 tactgttctt gaagctttcg aaaccagact cactgaagtc tatcgttttg aggccacagt
    10381 tctcagccat agatagtctg ttcttatcga tatcggaaat gtatgtctcg catttcatgt
    10441 ttttcagagc gtttcctagg agcaatccca taggaccgct tccaacaacc aggactctgt
    10501 cttcggcggt tatcctggct ttgtctatgc catgaattgc acaggcgagc ggttctgtaa
    10561 gggttgcggt gctctcgggg acatgattag ggatcttcag agttgccctt ttcaggatct
    10621 ctaatggaac tttgacatac tcggtgaatg ctccttcgac ccaatgtttc tttgagcaga
    10681 gctcgggaac tcctttctga cacatttcac atttgccgca ctctatgtac ggagcaatca
    10741 cgtagctagc atcaccgaaa tcgtagtcta ccgacgctcc cttttcaact atctttccgg
    10801 tgaactcatg gcctagaatg caaggcggtt tgaacatgtg atgccctttg ttgaatgtct
    10861 ttatgtctgt gccgcagacg ccaaccatac ctatcttcac gagaccttcg tctgcctgga
    10921 taacgggctt gcttatatca tcgatttcga tcttcccgat gcccttgtag attgctgctt
    10981 tcaaatgaac cacctcttat cgcagataat gggggagttg cctcccccat cacgaaatca
    11041 catctcaacc ctaacttcga ttacgtacca tgcccagttg tcagacggat acttgtcggt
    11101 gatctccggg aagtcgttga tcggatatgc aagctttata ggaccgccct ggctcgcagg
    11161 aagagccctg aggttttctc ccgatctggt tgagtgagtc agcattatag gatacttcag
    11221 tgcgtcttcg taagctaccg cgaattcttt ctcgtcggag cagaccatca ctacgttctt
    11281 cgcgcctgaa ggaactccca cgaacttcaa aagatcggcg agagtcactc caacatatgt
    11341 cttgtctccc atccacggat cggttacaaa gaactcggtt tggggcagtt ctttgaactc
    11401 atccagcgtg agttgccagg cgccttcctc atttctcaat gcgatcacgc cactaatttt
    11461 gattacagga tctgcgatta ccgcagcaga aatgagaagc attgccgcaa gaacaaccag
    11521 taaggctttc ttcatacgta tttcccctcc ttattcttcc tgaacaattc cctcggccac
    11581 caacagcttg atcggatcaa ccactctctc gctgtttcta aatattccgt acagagagta
    11641 tttatattct tttgaaatgg tgaagggaac gtccagattg gttatttgaa ccatgaaagt
    11701 atcactacca tcgttgaatg tgtaggtttt accccactga ccggtattgg cacaattcga
    11761 gacaagcaca agtgatccct cgaagtattc tggattggtt ttcatagatt tgatatctac
    11821 cgaagcgctt ctaacgaaga gcttctttct gaactctcta tctccagagt gaacaactat
    11881 ctcattcagg ccatcgtcac aggaagaaag attgacgttg aagtagtact cgctccagaa
    11941 tctcatctct ttcttcaaaa catcacattc gacgacacgg tcatttagaa gtactacgat
    12001 atcttctgtt gaaggaaacg tgcagaatct gatgtactcg actcccgaat agggggactc
    12061 tctgctcatg tcgaaccagc cggaacgatc gtatgtaaga gttacgaaac agtaatttaa
    12121 atcgcttctc ttcatctcaa aaagagtgaa cccatactcg tcaccagttg gagagggacc
    12181 ttcccaccat gctccagaaa cggcgccaca ggttagctcc acaaatggag catcgaagta
    12241 ctcaatcatt gtgtgcttgt gaccggtaat gtgatatgaa cagtcatcag caaaaatctg
    12301 catcagttcg tagtagttat cggtcttggc tagactgaat accggttcat gtgaaaagat
    12361 cacgattcta ttattattgg caactagctc aatgtccttc ttcaaccatt caagttgcgg
    12421 agaatcgatt tcataaacga aactgccttc gatgatgttg tgagtattaa gaactatata
    12481 gtgtgttcca aagctattga aagagtaata ttcgggtccg tagacgttgt gaaaaatgtc
    12541 gtacggatcg gccttcttct tgtttgtctc gtgatttcca acggagtaat atacagggat
    12601 attcgagatg gttagatagt ctttaacctt tgtagcccat ctggttgcaa tttcgtcatc
    12661 gatgtctttg atacttgcac ccatgtctcc gttgcagacg ataaaatcgg gcttgactat
    12721 ctcgagaatc gaaacaagct tggacatgta ttcatctggg ctgtcaatca tttctctgtc
    12781 ccctagagat tcatagaaga ggtcggggtc atccgcgtag tgaatatcgt ttacaacggc
    12841 aaagatacca ttctccacgg ctggcatcag taagaaatcg tgattctcaa gtcccttaac
    12901 ataccattcg ctgcacacat aaccgtccgg tttgatcagg aatatgaagc catccgaatc
    12961 agagatactg tagaacccct cgctgtctga agagactatc tcaattccat ttgaaataag
    13021 gcaaccctga actgtctgat cctccgggtc gagtatccca tttgagttca gatcaacaaa
    13081 cacatggccc tcaattccga aaacagcgct gagaaaaaga aataggaaaa ccgtcaagag
    13141 tgctttcttc ataaacgcct cctcatttcg gactagccgt gaacagtttt cagaagtttt
    13201 tcgacatatt cggtctgatc atcggcttga aatacattcc tgccgtaagc aacccccttt
    13261 gcgccgagct tcagagcctc cctcgccatt tcgaaggcat cctttccctt cggtccgccg
    13321 gcaatcacga cgggcacagg gactgattcc agaaccttcg taaatgactc aaagcccgta
    13381 taatagactt ttacaagatc ggcacccagc tcatacgcta ctctcgttcc tagtgcaata
    13441 tactccggat cagtaaagtg atccgtggcc tgcggaagca tttctgcaat caagggcatt
    13501 cctagcttgc tgcatttttc tgccactttt cctataattt caagcgacgc gttttcaccg
    13561 tctccaccta tgaatcccat aacaaccaca ccgtcggctc ctattgacaa cgcatgttcg
    13621 acggaagtag tgattctgtg gtagtcaaac tttgtggagt agttagtaga ggctcccgtt
    13681 attctacata gaatcgaaac ctttcctcca agcaaatctc cgttcttttc gatgacgcca
    13741 ggatttagaa ttattgcatc gggccggcca gcgactatgt tgctcaacgt cctcctgaca
    13801 tccctgatgc cttctatcgg accctggaac tgcccgtgat ccatagcgac gattaccgtc
    13861 tttccgtctt ctctaagtat ccggttcatt ctaatcagtt ttcccgtcat gacatctctc
    13921 ctatcatttg ttcagaacat atgtacctag attatagtac attccttaaa gaaaacaaaa
    13981 ctgaagcaaa ggatttggta gtttcatttt gcgtgaacaa ttaggaatag gagtgaaaat
    14041 tggtttcggc gtttggacac atgaaggttt actactgttc aatttccgtt cttttctact
    14101 gcagcagata atcaagttct gggttagctt tgacaccaga tgattccgga cacggttcta
    14161 tgatggaatg aagattgtta cagatgcctt tactttgctg ttctacagat tctgaagccg
    14221 agatttgtga tggcgtgatc tggcaaaccc ttagatctca catcgatact gacaccgacg
    14281 gggcagtcta tccagctgcc acctctctta actctgtaag ctgagaagct gtcaacatat
    14341 ggattcgctt cttctgaaca ggagtagtct gtgtgataat cgctaaccca ttcccaaaca
    14401 tttcccaaca gatcgaagat tcccagttcg tttggagctt tggttcctac cggatgggtc
    14461 aagttctctg agttagattt gtaccacgct accaggtcaa ctgagtttcc taccaatctt
    14521 gtgtagcctt cactcgcgtt accgcctctc gccgcaaatt cccactctgc gtctgtcggc
    14581 agcctgtaac ccttaacttc ggagggatca gtcgttcttc tgcccattgc atcaagaata
    14641 tggccctcgg agtcataggc taggggtaac ccttccttaa agctaagcca gttacagtaa
    14701 gctatcgcgt cccaccagct tacgttgatt gctggtctgg agtctcttcc ccatccggaa
    14761 tcgtcaggaa cgtttcttcc tgtttcttca cagaaggtat cgaactcgtc gaaggtaact
    14821 tcgcgcttcc caatgaagta atcataattc agattgatcc gtcgaaccgg ctcattatcc
    14881 acaccttcct ctctccatgt cgttcccatg agaaagcttc cgctatttac gagaacctgt
    14941 tccggcattg aagcacagag gaaagaagtg aaaaggacca taatagtaag cgagagcaat
    15001 ctgttcatgg aagcagcctc cggcacagac agtctcctag aatacacaaa tcctagagac
    15061 cgcgatgatt ttgtcttcgc tactagtata tcagtcgaga ggatcgtgaa gcctttactc
    15121 aggatttgag ctttcggcat taccaaatcg ttcgtggaaa ttgatgtgtt ttcaagaatc
    15181 ttcgtattga gcgtgacata ttactttagc tgctgagtga atcgcaacct gctgttagaa
    15241 agaggtctcc tggaaacgaa gtgtctcttt caaatctgtc ttaacctatt agctctggtt
    15301 ttgaagtaga actaggccga aagtgtgata ttgatctgca tataaataac tctgttattg
    15361 tttgttagaa acaagaagca agatcttcta ccaacctctc gatttttctc tctcgaccaa
    15421 agctccacca tcccacgcta ccctctccgc agatctccgc ccctttctct ctgcagattt
    15481 tcttcatgta ggtcagtgtc tgcttccttc ccaaagcggc tggaaagaga cccgtagcca
    15541 taacggcaac cttcttgcct tcaagggact tcattttggc aaggtaactc ttcatggcat
    15601 ctgcgggtgc tccgccatta actggagagc aaaagatcag cccatcatat ctttcgagat
    15661 caggagtctc cgatctcaac cttaccatag atgctgcttt ccctgcagaa gtcaacttat
    15721 ctcttagctt ttcagcaaca tacaaggtat ttcctgtttt tgaataagcg attattccga
    15781 ttctcacgac tatcacctgc ctttctggtc agtattccct cgactttgca cattccacac
    15841 attgtcgata ggttccttct caaaatccag ttccatgtgc gaggcattgg cccaatctac
    15901 caattcgagt gaaagtgatc cgttatctct ttgagctttc acagttgtta ttgcgcagtt
    15961 ggttatagaa ataccagatc ttgagaagaa actcttaggg tcgatcccgt tgcagacgaa
    16021 gggaagtgtc attgacagaa aacctccgtg actaacggcc aggacttctc cttcgggaca
    16081 ttcccttacc actgtgttca tagccttcca gaatcgatcg ataacttctc tgtgactctc
    16141 tccattgggg attttctcat ctatcctgcc atcaatccaa cttttaacga gttcaaggaa
    16201 tgccctttct gcttccccct gaatgagctg accctcaagt tcgccaacgt gaaattcacg
    16261 gatctcatca agtagttgag atttgattcc aagatcagat gagactattt gtgaagtctc
    16321 ccttgctctc agtatgggcg aggtgtaaat cttgcgaata ttcctgagct tcaagtggcg
    16381 cgcggccatt ttcgcctgga ttctcccatc atcagtcagg ggaaaaccat cgtcaccatt
    16441 tgcgaatacc ctttcaacat ttgcctgact ctgtccgtgt cgcatgagat atattgttgt
    16501 cattttttgc ctccgtgaag ttactcaaga agatgataac agatttggtc tgaaggtttt
    16561 ttggtcaaaa ctcacagagt attatgcgat aactcagagt gctcgacccg ttttgatgag
    16621 acttgggttc cagaggtcaa tatgtgagat gaatatatcg attctaaggc agttttagaa
    16681 agataactct tgcccacttg cggattttac gaatatgtcg gcctaacgcc tagttgcctc
    16741 ccatgttcct tcggctgaag gttcgaatct agtgaaggtt ccggacattg ttcctttctc
    16801 agcgactcca gcgaaatcta ttttgctttc agaggtccag gtgttgatat ttaccctgtg
    16861 atcgttggag atcatcccaa aaagctcttt acaggttgaa tcaagacaga attttcccct
    16921 gtagttatag attatgtgct gctcgatatg aaaagtagat gtcttcgagg ctccaccaga
    16981 ataagtcatt acaacatccc agcttccatt caaattagga agtccaaaga acagacaccc
    17041 agatgcaagg aagattatcg ccaatatcga gagtgcggtc aaaaagcttt tcttcattct
    17101 caatcccctc cgtgtaaaaa gcacattctt ttttgggcta actacggaat ctacaaagag
    17161 cgcgaatttg ctccaattat acagtgaatt aagctttgaa aggggaatct tagtcgctgc
    17221 tgaacagcga ttttctatga gaaaagacga tagtactact gatcaaatat agcaattccg
    17281 tgtagttaaa aagaaactat gaagcagagg cctcggagac aatatcatgt ggaagcgaca
    17341 taatttatgc tgatattatt tgaacattac gcctattgct catgttgtga tggattcatt
    17401 tttgcttgga agttcattag atattgtgat ctaattaaca tgaattagag gccgggcttc
    17461 ccatacaatc cctattcatc ctgacttcat ctaccagttt ttcgacgatt gccagacggt
    17521 tttagattca ttaaaggtca atgatagagc aaattctccg agatgatttc cagaatcttg
    17581 tgggtctaga agaatatgcg tgcggcagga tattgagttc catcatccat aataatgatg
    17641 agaggaggaa gtgaagtgtc cgggaaaaaa ggaaaattcg tgtttaacgc ggactttatc
    17701 tacaagatgc cggtgcattt cggtggggcc ccattctacc cggttagagt tgtctacggt
    17761 gataatacag ttatctgtgt tgactatgaa acagacgaaa atgctctatt aagctacatt
    17821 cccgaggact tcgaacttaa ggagccggtt gtcagtgtcc agtataccaa ttgtcgggat
    17881 gtggactgga tgattggggg agagtacagg ctcattcagg tgacgacacc tgtaaagtat
    17941 gttggaaact cagagggtct tgaaggagtc tacgcgcttg tagtgtggga aaacaagacc
    18001 tgcccgataa tcgggggacg tgaagaggat ggagtaccaa aggtgtttgc agacatctcg
    18061 tccgagcgtc atctggagaa tcactggttt gtggcggcca gctacgagca gttcacattc
    18121 ctaaaaatcg atcttcagag aggtgatgag gtaagtgaag aggatctgtc ggagctaaac
    18181 aaggattcga agatcaatct gtttgggtgg agatatctgc ctaatttggg aaagggtggt
    18241 gctactctga gccatgctac tctgtatcct caagagatgg tgttgagaaa aatgtggatt
    18301 ggtacaggcg ctgtgaaatg gactggcctg tcttatgagc aacatccttt gcagacaagg
    18361 atcataagat cactggaggc tttgccgatc agaagggtta aatcggcaag tatggcaaaa
    18421 ggaattgtga agttgaatgt cggcgattcc aggatacttc catgaattac cggaggagga
    18481 agagatgaac gaatactttg aaaataaggt tgcggttgtc actggagcgg cttcgggaat
    18541 cggactcggt ttgaccgagg agatgctctc gaggggcgcg aaggctgtat ttatggcaga
    18601 tgtaagccag gaaaatctcg atagagaatc ggaaagactg agttcaaagt atgaagggga
    18661 agcagtacca ttccttaccg atgtgacaaa agaagagcag gtagagaagc tcatcaaatc
    18721 agcaaggaag tacgaaggtc atcttgactt tgtcttcaac aatgccggaa taggtatgac
    18781 tattccaact gagatggtga ctctcgagat ctggaagaaa gtgatagata tcaatctctg
    18841 gggagtgatc tacggaacgt accacgccat accgattatg agggagcaga agtcgggcca
    18901 cataattaat acagcttcca tcactggatt ggttcctgtc ccatatcagg cgctgtatgc
    18961 atgctcaaag agcgcggtta aaactatgac tgaaagcctc tattatgagc ttttcaatga
    19021 aggattgaaa ttcagcgttg tatgccccgg aaatgttaga acggctatct tcggagggct
    19081 tactcctcca ccggactcag tatccgttga agaagcggtg aattacatag tcgaaaaaat
    19141 ggagaaagga gatctgctga tagtcttccc ccagaaaatg cgcgattttg acgagcttta
    19201 tcgaacagac agaggaaaat ttgatgaatt tgctttcaat ttggcagctg aaagacggga
    19261 gaactacaga accaaaggaa cttattattg ataaggtctg atgcccatga atctgacaaa
    19321 ggtttttgat gaatcggagc tgtctctgga agtagtaatc ttgatgattg ccggactcat
    19381 cctgcttatt acagggatac tgctctttcc tgtggctacc ggaggacttt cttattatga
    19441 gaatggtctt tacggacttc tgctggtcat gttctccctg cagatcatct caatggggaa
    19501 aacccccttt ggcgacttga aacgatcgaa gctagtcttg ctggcgggac ttgtcgttgg
    19561 ggggattgga acgataactt gttttgttcc ggatgccttc agcgatattc ccagatttat
    19621 gctattcctt ttcttcggcc cgggaggagc tctattgctt cttcaaatga tcctgtcgaa
    19681 ggataagctc aaagcctggt ttgaatacgg tggaatcttc aaacacttga ttgccggatg
    19741 cacggtggca tatgtttcat cgattctcat atcaatactt ctttggaatc agagcctatt
    19801 gagtgtacag atgacggcga tctttgttct gatctatgga gcggctatcg catatctttc
    19861 gtttgtcctg agaaagatat acagtagtta cccgcaagaa cagaaaagaa aggatggaga
    19921 agtcgaactt cccatcgaca gggctatgat tctatttaca agtgttttta tgattattct
    19981 gggcgtcctg ttgataccgg taaatctagg tctcttaccg ttctcaggaa gtgcccagct
    20041 tggtcttctg atgatgattt ttgcgatcca gatgattgct tcgggaagca ccccgatagg
    20101 ggtctttcca aggtcgctac ctgtaatcct tataggtttt gtattcgctg cccttggaac
    20161 tgtgtcgtgc atcatcccag aggttctggt gtatcctctg accgttcttg ttggggttct
    20221 aaacattcta ggcggagcga tttcaattgg caagttcctt ggacagcggg cgagcagtac
    20281 cgatcaacag aaaggtaaga ttccaaagat actggtgaag ttgaacgtct ctcagctgat
    20341 tctgaacgtt cttgcaattg cattcggttt atctatgttg atctctcatc tcttgccggg
    20401 actggttatt ggcgtggttt tggcggctaa tggggtggtg cttctgtatt tgctacacat
    20461 tttgttagtc atagatagaa tacaaaagga gatggaaact agataatttt agagtattat
    20521 caaagctaat caactgaatt cagagaaaaa acaccggttg gattggttca taccgaaaaa
    20581 cttcattctt aacaacttga tagtactttt ggaagctgtc gtccggaaag tcggcggtca
    20641 aattatggat cattcatgaa gcaatgcaga tcctgatctc ttgagcgtta tttgaattcc
    20701 cgacttttca gtttcatcga aagtgactta actctcttta cctgtaccac gaacgattca
    20761 agtcggcttt ctacagttgg agtgaacggg tttccatcgg actcaaagaa gagaatcgga
    20821 atattgccag cgttcttgaa gatctccctc ttgagtttgc tggagttttc tctcaggtgt
    20881 tccagacctc tttccgcaat agcttcggcg atccttgtag gcatacatcc aaatgggcct
    20941 atagaaataa ctccatcgta agtatctatc gattcgaaca aaacggctcc aagagtcagt
    21001 attgcctctc cggtaagctt cggattcaaa aagtctttag aagtgtttac catggactcg
    21061 atgtcgacga aatgtgattt gtacagtcca ctcttttcaa gaatcttctt gattcttttt
    21121 tcaaatgact gttttacaag ggtttcaacg tgtttcgttc ccctcttgag cagaccgagt
    21181 tcggtaggta cgtatttctt cagcagaatg taatcgagat aatatatcca ttcttcaatt
    21241 ggagaagcat gcatgattat tccgttgtca gcaaaaagta gctccagaga cttcctggaa
    21301 aactcatcat tcctgacgta aatctctccc gagagaagga ctttcggtgc actttcgaaa
    21361 tcgcccttta gaggaatgtg agagatactc tcgcaaatct cctccagttt cttggtcatt
    21421 tcggtaaagg agtctctttt gacactctca agcataatct tgcgggcctc tttggtcgct
    21481 ttggctgctt catctctgtc tgatgccagt gtaagcaagg cattttcaac attgcaaaat
    21541 tcgtcggcaa taatcatgct cgcccagatt cgtaatttcg ccttgagtcc tatgccggca
    21601 taaccgtttt cagaattgag agagaacagg agaacgttgt tcgcgttgtt cttgtctagc
    21661 caaagatcta tgtaagtgct gtactgtcca aatcggcaag ggccgcgagt gtcgggcatg
    21721 aagtacatta atatgctttc gtcgtccttg ttttcctcga ggtaacgaat taggcttccc
    21781 agagtcaagt gaaaaggcaa acactccttg gataatgagt tagattttcc cagttgaaac
    21841 tctaattctg tcggtctcgg gagaacgtga gtgttaatac cgaccttttc gaatgccgcc
    21901 gctatcagct gggccgcaaa ctcgcccatg gtagggacaa ttactttcac agaaggatct
    21961 gtaatgggta tgtgtcttcc atcctgcagc tctatgctga atgtcccgtt gttcgggaga
    22021 agttttggtc tcttcgccct tttcttctcg cccaaatcag tctttctttc gagggagctt
    22081 ctgacgacat cggcgaaggc ttcgatcctt gtctctactc cggcatctga cgtatgacta
    22141 tccagttcca gaatcaggga cggctttttc cccataatgt ctctgaagaa ggaaatcaag
    22201 aacgagtcag gtccacagct gaagtttgtt atgtagagtg cgaatagctt tggattgttc
    22261 cttacatatc tggccgcttt caagttcatt tcaccccagg cccagtacat cttctcatac
    22321 gatctttcgc cttcataagg aagagcgtca aaagttacca ccgggattcc gcgtgaagct
    22381 agtttttctg gtatccccat gttggcgtca ctggaaaaag cattgtaggc tcggccgaag
    22441 ataacgacac caaaatccga attctttagg ttctcaagaa acctctctcc aatttgcttt
    22501 atccttctta cactatcttc gtaggatttc actccgttgg aaaaggctgc caaagcttct
    22561 tcgcggttcc ttcccattga cttcgcaagt gaaacgaact tctccttctc tttctcaaag
    22621 ccctgcgaaa agtcgaagaa atctgtcagg atccttttcg atctgagttc ctcgaaactc
    22681 cccattagcc agtccggctc gctctggaca aatggacaga agaccgagtt ttcaacggag
    22741 ttctcaacct ctaagccctt gactgcagga atgaagaagt agtctggatt cttatccaga
    22801 agatcataag tataaccatg cgatagttcg actggaaaac agaattcaga tttctttcta
    22861 tcccagcccc tggggtcgga cttatccggt acgacaattc tgaacccgag actgctgaag
    22921 aaattggaga acagggggaa gagggtattc atcgagagag acttgctgat tccaatagtc
    22981 tccaggccct tttcttctgg cagcttaaag actatcttct ctctttcggt agtatatatg
    23041 tattcgtttc tgtttatagg aaggttaaga cgaacatttt catatttgtt gcaagcgccg
    23101 ccgaatggaa acttctttcc tccaacttca attattcgga tcgggcactt cctgtcacac
    23161 ttctctgctc caccgtcaca aatgaaggtg ttgagatagc gtacttcttt tgagatcaag
    23221 gcttcaagct caaagcgttc ttcttccagt tgacccgctc gaagcttttc cttaaccatt
    23281 agtgatacac caaaggcacc catgagtcca ggatgaggtg ggacgattat ctcctttcca
    23341 gaaactgccg ccattgcaac tgggacagct tgattgagac atacacctcc ctgcatgaag
    23401 actttcttcc caaccggtct catggctttt accctgtttg tgtagttcag acagatagag
    23461 taaaccaagc cggcgcagat atcttctctg gagataccct cgttgattgc agtctttatg
    23521 tcgctgctga taaaagccgc acactgatca ctgaagtttg ggggagattg acccttgagc
    23581 gcatactctc caatctctct gtaatctatg ttcagagatt cctttgcgga ttcttcgaga
    23641 aacgagcctg taccagcaga acaggcttcg ttcattgcat agtcaagggc aacgccattg
    23701 gataagaaag tgtatttggc atcctggccg ccaatctcaa atatcgtgtc gacttctcta
    23761 tcaaagaatg aggcagccct tgcgtgagcc atgatctcat tgtagacggc actcgtttcg
    23821 caataaagac ccactatctt ccttccggaa ccggtgaccc cgagaccaat tatctcgatg
    23881 ggaacgtcca gtgaattaca gatctcatcc aggcactgtc gggtagcgtt tatcggatct
    23941 cccatagtcc ttagatatga actcgctaga atagcattgt cccttgctct cataaaaact
    24001 gccttggtcg tagtcgatcc aacgtccacg ccgagaatgc aagaatcgcc gctttcagct
    24061 gttttgaaag gcatgttctt gaaatcaacc atgttaacga acttcgaaag gggctcgttc
    24121 ttcgggaaag tcattactcc ttccttgaag acactctctt tctctatctc gatttcttct
    24181 agagtattgg catgtagata ggcaccgaag gcctcgaaga agagagcctc ttctggtatg
    24241 gtgatttctg cgatttcttc tagatggttg agcatgagtc gattttttgt tactccgcca
    24301 actagaagta tcttctttac tccagctctg tgcatgagtt cgccgatctt atcagacatg
    24361 acctttccaa ggccattgag aaccgattct ttaggtgttc ccttattgag agcatgtgtg
    24421 cagtcgctct tgcagaatac ggtacacctt gatgaaagct cgtaaatctc gtctgtttcc
    24481 acggcgtttg cgtcgtcgag tccgatatcc atacgcttca gttgctggag aaagaactcg
    24541 ccagttcctg aagcgcattt actgcctgtg aagacggcag taatgtttcc cttatcgttt
    24601 agcttataca gggtgaaatt ctctccgccc acgctcacaa ttgcctcatg atctccgtac
    24661 ttgcttttca gttctttgta ggcaaattca gtggcctcaa cttcggggat ctgggggaga
    24721 ttcaacagtt ttcttccctt ttttcctgtt accacaacca aacccgtttc aagaatctct
    24781 ggaagaaagc tcttgagtac tttcagtgga tttccttcgt gcaagagtct tctgggtgtt
    24841 tgcccatcat accaggaaat agtgcttgag cctatgcaga gtccgattct attcaagaag
    24901 ttcacctcaa accattcaac agtttaataa tagcatagta agacatcatt gtatttcagg
    24961 aacgcaaaaa ccttttccta tgtgacttgc agctctttaa gtagacttaa ttttcggaag
    25021 gttcgccttc aaagaaatgg atttcaataa ggaactattt cacctatttt ctgggtaata
    25081 gccgggtaat ctgtttcatt caaatcttca cctttcttct ccacttcaaa gagacgaatt
    25141 ctctatatca gtagggtgtt cgaattaaga cataccagac tagtctcgcg gtctttcttt
    25201 ctccactcct ggagggcagt aaagaggatc aagggctctt ggtgaaggca ggtacattct
    25261 caaggacaaa gaaaaaggtc cattgggtgc ggggagccaa tttctttcga gatcagggcc
    25321 tggcgaatca tgctggatat agatgtcgag agatccatca tcattgtatg acaatctcga
    25381 ccgatcacca atggaatatc tgtctatttg gttgggaacc atgaactggt catcaccgta
    25441 cattgtgatg gaccagaacc cgccgggttc tacgggaggt atttcatggc tttcaaaatg
    25501 aatcttgtaa tcgtactttg agccgtcata tgattcattg tctgcatcga cgtaacttat
    25561 tgggtaatag gcctcttcga gatcatttcc atacagacct acatatgctg cggcagccct
    25621 aatctcgtat tttccctgca tttgttgccg actgccaaat atccgtttcg ttaaattcca
    25681 tccgttcttt gtaactccca gcttctctaa cggccggaat attgtactca ttgcatcttc
    25741 aatccccttt tcaatcgcgg tgatcatctc tggttgcaag tcattctcat taaatggaag
    25801 gtttgggcct atgccgatta gaccaaaacg ctcaatgaga gccttttcag aaggatgtat
    25861 ctcaagctgt ccaagaagga agttcaaata ggaaatgaag cctgccgatt cagccttttc
    25921 ctgactgaaa tgcgggaagg ctatcgattt gttcgatgaa gaagtttgac taccgtaaaa
    25981 tgccccgaag ggttcaatct tgaagttgtt ttgaatcttc ataacagcat caatatctcc
    26041 tggcatttcg ggatttacgg aaatccttac tatgcagaag acgaaattcc cttcgctagt
    26101 gaacacctca tcgatttcgt tgggaatctc aacgaaagag gacgggccac tgaccattac
    26161 tgtcaatttc ctgcaaccgg tggttctcgt tcctgcgtat gcgaagttat gtgtgtacat
    26221 gtcgatgaac tggactgaga aatatctttc acagatactt ggaatctcta ttattaccgg
    26281 ttcgtttctc agatcaagcc atgcagccga gaacaacgta tcgttgttcg gtcttgctat
    26341 ttctctgaac tccggtccca gtaatccttg aagatgttca aactcgttga atgaatttgg
    26401 aatcactgcc tggagaacca tagttctgta gttctcaagc ataggaaaag cgaaaatata
    26461 cgcttgcctt gcaacttccc ttgcttcttc gtagttgctt gttaccgaag tcgattctgc
    26521 accaataatg acagcgaaaa ccagaatcgc agatattaat gccagagagt ttttcatgcc
    26581 catttctcaa tctcctcacg caaccgctct ttgccgacta ttctcggtcc ccagttttga
    26641 acctaggatg ttcagcagtt taaagggttc aaacctcagc aaaagatact tgtagatatc
    26701 aactagtatt tgtctgactg tccattgaaa gaaagagttt gcttttcttt tggatcagaa
    26761 ttactaatcc cacgacaaac ccatctcttt gatttctaaa caggtcttca ctcacaatta
    26821 cagcacgtgg aaagcttcag tgacaatata gtgaagttac ttacatcagt acagcacaag
    26881 agtaataata aaccccttga gtcactctgg acgttcaaag aatcctgaat cggcgtaacc
    26941 atcaacttgc ttgactatta ctcaacgtaa tagtatatcc ttgtgattat gggaggaaat
    27001 ttcttgaacg caaaacaggt tgccgagctc tcagaccttt ctgtcagggc gcttcatcac
    27061 tatgacgaaa tcgggctctt gagacccagg cgtaatgctg aaaatggata cagagagtat
    27121 tcggaagaag atctcgacaa acttcagcag attctcttct tcgaacagtg tggcttctct
    27181 ttggagagga tcaagaagct tccaagcaac ccgaggttcg atagagtcaa gaccttcgag
    27241 cttcagaaga aacacttgat actggagaaa gagagaattg aggctatgct cagcacactg
    27301 gaaaggacgc tcaagacttt gaaaggagaa gattcgatga gtcagagaga aaagttcaaa
    27361 ggattcgatt tctcctacaa cccctatgaa gaagaagcga aacagctttg gggtgatgag
    27421 acagtcgaaa agagtaacga tttcatcact tcgaagagcg atcgggagag gaaggacatg
    27481 gagaagatac tggatgacct tttcaaagga ctggcagaga ttcggatcaa agatcccgat
    27541 tcagatcttg cccaggaagc aattgacaga atgtatagag tctttaacga tagctttgga
    27601 cattactata tttttgagtc ttttgccgct ctagatagca tatgtgtaat ggatgatcgg
    27661 ttcaaagcaa acacagataa gtatggtgaa gggttagcgg agtttctttc aaaagcgatg
    27721 cgggagtatg cagacaggga atctgaagga tgaaccgaaa gtgtcggtga tagaactggt
    27781 tatctatgat catgggaact atcacctgaa ttcgaccagt ttcaaacaag agcaaagcta
    27841 gctgctgaga taggttcgaa gacttccaat ggtctcttcg atttgccttt tgtctatgca
    27901 aggtgagcaa caattcagga ggtactccta actcctgacc tgaaggcgtt gcgaccagtt
    27961 tgtactggct aattcatctt aacgtcgcaa ttgaagagct ataattgtgt tacttgtttc
    28021 ggattgtgtg accttccgca gaagctacta taagcatgat ccacatttat aagataggat
    28081 catgaataac agaatataaa caggcagtgg acttgcctca tgagcggggg agttctttga
    28141 agaggagtat cgcttttcag ggtgaacacg gagcttattc agaacaggct ataagaaaat
    28201 tgttcgggga atcacctact acttttccat gcagatcttt tcgggagatg ctgaaacttg
    28261 taagcgaaga aaaggtagac tacgcaatgc tgccggtcga aaactccctt gctggtactg
    28321 tgattccagc atatgatgct ctcatagaaa gcgagctatt tgttcatgcg gaagtcatgc
    28381 ttagaattga gcactgtctg atggcaccag aaggggtaaa aattgaggac ataagatacg
    28441 tgatctccca tcatcaggct ttgtcacagt gcatgagaca tattgaagag gagggtcttg
    28501 aggcgaaaga gtattatgac actgctggcg cggcaaggga cttgggcact ttaaagatgc
    28561 catttacggc tgcaatagcc agcgaattgg cggccaaaac atatgggctg gaaatcctta
    28621 gaaggggttt cgaggacctt gatacgaaca cgacgagatt cttcttgatg ggaagagaag
    28681 ccagaaagtg cgagggcagg tgcaagactt caatgatatt taccactgag cacaagccgg
    28741 gagctttgtt caaagttcta ggagaactgt cggatcgcaa tttgaacttg acaaagatag
    28801 aatcaaggcc atttacgaag gaaatgtggc actatctctt cttcgttgat ttcgaaggaa
    28861 gtgtatcgga aaagagagtt gaagaagctg tcaacgcaat aagcagtcgc tgcagctact
    28921 tcaagttcct gggttcctat cgatctgcaa atgatgttga gctaaactca aatcagttaa
    28981 catagataac gttaacacgt gaagtggtaa cggaaagtgc aggtaaactg attatgcggt
    29041 gaaaattatc ataagtatgt ggtaatcgat aattataaga gctatctatt agcagtgttt
    29101 cagttgacgc cttttcagaa ctctcaaaaa acagaggcct cctctctaca gattgccttg
    29161 aaaagaggcc caacttaatc tcttctaaaa tatagttatt cattcttcaa aatagggaaa
    29221 ctgagctatt ctcaaccgtg ccgcaccgta aggaatcaaa catactttct ctaacggccc
    29281 ctctggtctt acagggcttt ctgggagagt cccagccgaa ttcctttccc acgaccattc
    29341 ctttaccttg gctgcatcgg cttcaatcaa aactggaact cgattctgat caaagggttg
    29401 ttcagacaat tgttgtgttc gcttgataaa tgaaaagctc ttttctgggt tcttaagatc
    29461 cactttcaag gcgtaattcc atggactggt tgtataaacc tcatattcag gaaactcccc
    29521 gtctttgttc gtagtgttcc acttttcatc tagtctcaaa gcgaaaagaa gaggaccttt
    29581 ctcgacacta agcgagtttt tgaaccattt aggggttcta accctagacc ggaaaaccaa
    29641 ctcgacccta tcacctctgt tccataaacg ttttatggtt aggtaaccct cattgttttc
    29701 tggatcaaac gctgaaccat tgagagtaac ttcacttttt gttgcccatc ctggaatcct
    29761 gaagtgaagt ggtatttcaa aatcagcatt attatcaata agaatttcga ttttgtcgtc
    29821 aaaaggataa tcagttactt ctcttatcaa aatagtctgc cccgctacgg atgtttctat
    29881 cttgcaagga ccataaaccc cgaaaaccag tccgccgtca tcactcttgt aacatagatt
    29941 cttaacgaat tttggccacc cttgatgcat gttcgctgta cagcaaccaa agttcggttc
    30001 aagtccgaag agatttgagt catctagatt gttataccaa ttgcgtttgg cgcgactgca
    30061 cataatctga ttcacttgtt gatcgtattg gtgtgtgagt agctccttat caattgttgc
    30121 aggcaatgcg ttgtatgcaa ttttctcgag aatatccgcg aactctgtat cgttgaaaac
    30181 tctaaagagt atctcgagtg agaacatata ctcaaccacc gcacataact cagttcctcg
    30241 cgatggatcg tttccattta agtgttcgtc acctgttacc attccatttg atgttccgtg
    30301 gtaatcaatg agctttttaa ttccttcttt gactattttt agctgattct tctctccgta
    30361 gagtaggtac cttaaagccg gatacttaac acccataact acatttacta catgagtttc
    30421 ctgaaacaac ttagaatatg tagtgctgtt cgttctttcc tcgtattcgt aaagcccttc
    30481 gagtcccttt ccgcccgtag ctttcataaa tttctcccag tccagatact ctccggtcgg
    30541 ttttgtgtac ttgaagtttt cgaaataatt ggaccagttc attgactttt caaaaaacct
    30601 tttggctaga tctatcagga acccttctcc agtgatgcta tagagccaga gtatcacgta
    30661 aatattctcc acacttcttg tgttttccca aattgcgaac tctctggaat ctatattttg
    30721 cagttgatac atgaaatacc tggtcatgaa atgaactaca cgctgatcct tagtgtattc
    30781 aaagtagttc ttcaaaacct tgagcatgac cattcttggc caccaatcat cattatccct
    30841 gggcccgaag aacccctcct cgttctggct ttccaaagtc caggatatcc attttgctgc
    30901 ctttcccttc agtttttcat cctctagtag ataggccaga ggaatgagac catcacagta
    30961 atatggtcct ctttcccagt tttcaccgct tcccccgagc cagccgctat ccggtcccac
    31021 atctttccaa acctcttcca gtcttcctgt caaacctttt gcctgaatct tgagttgtct
    31081 ttccagccac ccggcaggtc gaatctcacc aagaggcagt tccataagct ccacatctat
    31141 cattgtttca cttcctttgt attcataaag cttttctatt ctttgttagt tcctgctgtc
    31201 atgccttcta catagtattt ctgcaggaga aggaacacaa gaatactggg tatgatgctg
    31261 aatgttgcgc cagcgtttat aagtccccaa tcgcgcgtaa atctagttgt agcgaataag
    31321 gtaattccaa cctgtatgtt tctaacgtca tttgagctgg tgaagtttag agcgagtata
    31381 aaatcgttcc aggtcatcaa gaatacatag actattacag ttgcaattgc agggagcgca
    31441 agcggtagat gtaccttgaa gagtattcgc atttctgaag ccccgtctat aagggcgctt
    31501 tccctaagag tatttggcaa tgaagagaag taattcttca tcataaaaat gcagaaaggc
    31561 atggaataag ttgtgtaaat aagcgaaaca ccaaacaacg aatctagaag tcccatcgca
    31621 ttcatcagat catacaaggg aatcagaaga gactgatatg ggaccatcat tatagacagg
    31681 attatcacaa agatgatatt gctacctttg aaagcgagct tgcttaagga ataacctccc
    31741 attatactaa gtatcagaac catccctata cttaatccac aaaggatgaa agtatttaat
    31801 acatatcttg tgaatatgga atccccggcc gtgaagagct tcgtaaactg ggctaaggtt
    31861 ggttgtttcg gtactaaact gggcggatat gacaaaactt cactcgaggg tttaaaggaa
    31921 gttatcgcag cccagtagat cggtattacg aaaattatcg agattatgat taacgtaatg
    31981 aagagtttct tactcattct ggaatcctcc cgccttcaac tgcaaataac taatgagaag
    32041 caggattccc aagaatatta ccgacaccgc cgagccaaat ccaatgtcgt aaaattcgaa
    32101 tccgattttg tttatgtaca ttacaatagt ctctgtagtt tttagtgggc ctcccttagt
    32161 cattatgtag aagtgatcga aggctttgaa ggaaccagcc agtgaaagaa tcaaagccag
    32221 taccagtgta ggacgaagaa gaggaagcgt tatgtaccta aagacttgtg atttctccgc
    32281 cccatcgatc gaggctgctt cataaatctg gggggaaata ccctgcaacc ctgcaatgac
    32341 aattatcata gtgaatccaa gagtcttcca tgcaaccatt atggaaacca taactcttga
    32401 caaccatgtc tgcccaagcc agtttattgg ttggcctatc aagttaagag atattaaaat
    32461 gtaattcagt attccatagg aatcgttgta aatccattgc caaatataac tagcagcaac
    32521 aaaggaaatc gcatatggta tgaaaaaaat cgttctgaaa atttttgtgg aacgtgaagt
    32581 ctttttcaaa gcgttggcca gcaaaactgc aggaacaaaa atcattgggg taactattat
    32641 tgcgtagatt acggtgttcc aaagggtttt ccagaactca tcatctacga gtgccttctt
    32701 gtagtttgac aaacctacaa aactcgatcc ccccattatc ttccattctt ggaatgacat
    32761 caacactacc ttaatcaagg gatagatgaa aaatatgcta atcatcacaa taactggaag
    32821 aaggaatatg aacccaaata atcccttcct tgtcttctgt ttcattggcg gttcctccta
    32881 aaaaaatcgg gatggtaaaa tgccatcccg atgagggaag aaatatttat tctgcaagta
    32941 tcttctctat ttcctttgtg cattctctta ctgctgtttc tgcatccatc tgacctagaa
    33001 agacgctctc aaagtaataa ccaactggat catagatttc attgtactta acagtgtatg
    33061 gagtttgcgc tatgccaagt atatctgcga aacgcatcat ctgggggatg tccttgtagt
    33121 attcgttatc ataaaggcta ggtctcgcca ctagctgtag acttggagcg aaggcaccga
    33181 cctgggcttc ttcagagaac gcaaattcca tgaattcttt cgccgcttca acatttttgc
    33241 tttgtgaggt tattccgaga aggcttccac caccgaaaga agcgaattcc cctctgttct
    33301 tagggatcat agctactcca aattccatat caggtgcatc ttgtagcaga gacgttatgt
    33361 tgaagttgcc gccgaggaac atagcgagtt tccctgaaac gaacgcattg tacctgtcgc
    33421 cagaaccata ctggacagat gaaacaggag caatcttgtg cttatgaaca agatccgccc
    33481 agaactgtag agcttgaact gtctcataag aatctagcgc tacgtttcct tcatcatcaa
    33541 acattcttcc gccgttaccc cagacaaagg gcatgaacca gaacatataa gcgccagctc
    33601 cgccacctgc aaggttgaat ccccactgat caatgattcc gtctccatct ttgtcaacgg
    33661 ttagtttctg ccccatttcg acaagttcgt accacgtttg aggtggctgt tcaggatcca
    33721 aacctgcctc ttcaaaaagg cccttattcc aaagcattgt tgataggtca atctggtaag
    33781 gcaatccgta tatcctatca tccttcatgc caatttctag cattcctttc gttaattcat
    33841 caaagaaagg tagttctttg gcccattccg taatatcagt gaacgcgccg attgaaataa
    33901 agaaaggaaa ctgaacaaga tccattccgt agaggtcagg agcaattcca gcggagaggg
    33961 ccgccatgaa cttcggaacc atttggtcgt aaggtatcag ctcaagcttc acttcgatat
    34021 cctttccttc tgccttcatt ttttcgttga acatcttgac tatgtcttcc gttgctgcga
    34081 gctggtccca catgacgagt gtcgtttttg ccattaggat gccagaaaaa gcaagagtca
    34141 ccaccaggat caataccaac agtcttcttg acataatgaa acctccttct tggattatca
    34201 gttgtagtcc tgtccgaaat tccatcactt gagaacgttc ccgggtgcgt taccaccctg
    34261 attctatcat agttttcgtt tcaatcaaaa ccagccataa ctaatctcat cgagtgagac
    34321 gccgtctatc tcgataacta cactgtttct tgctttagcg cgatctttcc acaccgaaaa
    34381 actctgatag aataatatta cttggtccag taatgaggtg atgctttgaa gaagaactac
    34441 gctaccatta aaatgattgc tgaagacctt aatctttcaa cttccacagt ttcgagggta
    34501 atcaacaagc atccagatgt ttcgcaagag actagaagga gagtgcttaa gaagattgag
    34561 gagttaggct atgtaagaaa cgctacagct acgcttatgc gaaataatgt aagcacgaca
    34621 attggagtta tcttcgaggc tgagtacgat ccgttctttt ctgagatact acagggaatt
    34681 gagaaaggta tatcgaagta caactataga atgattttga tcaacactaa attgagatct
    34741 gcagaggcta ggagtgcagt aaacatgctt cttgagcatc gagttggagg gataataatt
    34801 gtcccaagca atcccgagat ctcttatgct aaagatctag tcaagcgtca attcccggtt
    34861 gtagtaatcg gtagagatta caaagaactt gatgtagatg aagtttttac agacgactac
    34921 catggcggtg agcttgctgg agagtatttg gtatctcgtg gagcaactcg tcttctgatg
    34981 atcaattcca gagattccgg ttctgcttcc agtgagaggg aaaagggttt tgtaaagatt
    35041 gccgaggata agaaagtgta ctatgaagta ataaatgatc gtgttacgga ttacgataat
    35101 actttcaaga tagtgagatc tatcttttca gagtcaattc ctttcgatgg tatcttctgt
    35161 ttcaatgaca ctagagcctt tgcagctatc aatgctctaa ggagcataga tgagtcgttg
    35221 cttagaaaga tatctgtaat tggatacgat gacattatct tttcctctat tttctctcca
    35281 aaactaacat ccatcagaat agacaaagat atggaaggat tcgaggctgt gaagttgttg
    35341 gatcaaagga tcagaggagt aagaaagtca gtgaaaagag aggtactgaa cgtatctttg
    35401 gtaataagag aaacttagtt gcttcatatg ccgtttgtct tgctggttct atgaagaaat
    35461 gaagattctc ttgaatacgc gatttttcta aatacagatg cattaattct ttccgagaaa
    35521 tcggaatccc taagtagtca agatataaaa ctaatgctat cttcaacacc agagtcatca
    35581 aaatgaacag ccggagacat tcgcagtccc cggccggaaa agctttcttc gttaaagtta
    35641 ttactggccg agcatcttct ccattacttc tagcgcttcg tcagacagaa gtaccattgc
    35701 gagtctttga gtagtgaatt tgttttgcac gggtctctca actatacctg gtctgctggc
    35761 attgagcctg tcggtcatct tctctttcat gactctcgtt ctttcgtcaa catcgagatt
    35821 cttgaggcgt tcctgagctt cgggagggag atttctgaac tgctgtctca tctgttcctc
    35881 aatctccgga agtctttctt cgattctccc tataatgggt gcatttttct catgcacttc
    35941 agaacgattg gctaatggcc tttctctgaa gtattcagca agattctcgg cctgttcgac
    36001 cgtgataact gatttcagac cgctcagata attctcgact atcgactgca ttcttgtcct
    36061 ggttgaatca atttctggaa aagtcgcttc ttcgcctgag attgcttttt ccagagtttc
    36121 ttcaagagta tttgtgactc tttcctttag agcttcgagt tcgtcgcggg tggattttgc
    36181 aagatcgtac acttggccca gctgttcggg agagaggttg gctgcattta ttgcatttgc
    36241 aatctggatt tgcagacgta gttccaaaat ctccccatcc atatcatttc taaaaccggg
    36301 agaagctgtg gccactactc ctaaaatcag cattaggatc gagagaactg ctatcttctt
    36361 catttctgtc acctccattt gagactaaga ctattataag aacctgtaag tggaaggaat
    36421 gtgaaatggt ttaacgttct tattcctcat tcgtgataag gctcctcaaa gcaattgaaa
    36481 acaatattca atatggggga cgaggttgaa tccttttttg gggctttttg tcaaggttat
    36541 ttctaattga tcattttgag ttgttttagc acggattgaa gaatctggtg atctcctttt
    36601 cagctcgaat agacgggcat atcttcatct tttctacaat ggcgagaagg ccgccaaaga
    36661 tcaggcagag atgaacgatt atctgagcag actatacaat gatctggtaa acaacacacg
    36721 tgaagaatac cgcatgaagg actacgacaa atactttact gtttccagta aatccaggaa
    36781 aatcacaccg aatgaagagg cgatgcgtga agcggcaaga aactatggct atttcgcact
    36841 gctctccaat gaagtcaacg atcccttcga ggccttatct ctctatcgca gtaaagacat
    36901 agtggaaaag gggtttggga acctcaagga tcgtttgaat ttccgtagaa tgcaggtttc
    36961 ctcagaacta tcactgaatg gaaagctgtt tgtcgagttt gtcgccttga tttatctttc
    37021 ctacataaag aagaagatgc aggacaccgg tctcttcgag aactggacat tgcaagatct
    37081 ccttgacgaa cttgacacca ttgaaaggtt tgagtctccc gaacatggcc gcttgatcgg
    37141 agaagccact aaaaagcaga aggatatcta tgtcaagctg ggggtgaaat caccctcgtt
    37201 ataattttcg ggaatgtcgg cagcaaatga actcttgctt agaaagatct gtattcccaa
    37261 cgaaaacatc cacaggatca gaggtgaact ctctcaataa gaggctgtct ccgaatatag
    37321 aaacgagccg ataaattact tcagagggtg tgacgtaagt ttcgatcttg tcatactagg
    37381 gtttggagag aatgggcaca ctgggttttt ttcccgggtc gaaggaaatt agcgatgtcc
    37441 gttcccttgt tcttcccact gaaggaactt atgacgagag gccttcaaaa aggattactc
    37501 tcggtcttcg agcactgaat cagtccagga atgtcatcgt tttgggcacg ggagtctaga
    37561 aggccgatat cgtcaagaag gtctttcttg acaaagattc cgaactgccg gtctcaaaga
    37621 tttctcccga ggaaggtaga ttagtctggc tgctggataa agacgcagct cgatctctgc
    37681 catataaatt tctctcgcag tcttcatgac ggattccaaa atcatttgaa agaagttgag
    37741 atccccaata ttcatcgaac tcgaagaaag atcttcttcc tgaagagaca acccttgaag
    37801 cgtatttctc cttcactttc actctcatta agaaaacttc ctgttcactc tcgtaagatc
    37861 cgtaaccatc acttcgatca atttccaata aacgcaggtc cttcatttag agcaaagaga
    37921 tttcgctcaa tcaacttctg acactgagaa aaataacagt gtttcgattc ccgtttcttt
    37981 aaataaagtt cctgtgatta gcgtgataag ctgaaaaagc tctattcgga cgaaaataga
    38041 atcacaaaag actgcctttg cggaggaatt cgataaatgt tgggattaat cgatcgcctg
    38101 cgattctagt cagtccagaa tagacctcaa cgagtttaga aaaatgaccc acaagggaat
    38161 aaaatttcat tgaaagaaat cacgtttcat taaatgaaat attaggaggc gtatttatgg
    38221 acgttgttgg caagtttgga aggatcctca taccaatgag cacagcattt gcagctgact
    38281 attccattga ctatccaatg acaagaagaa ttgccagtta cttgatcagt aagaactatt
    38341 gcgattctct ggtgatagcc ggaacaaatg gggaattcta ttcgatgagt tttgaagaga
    38401 aagttagact cttcgcagag gtcaaacaag aggtcggaga aagggtgccg cttatagccg
    38461 gtacgggcac tgctaacact cacgaaacaa ttgagttgac aaaagaagcg gcgagactcg
    38521 gttatgatgc agctatggtt gttgtgcctt attactgtca ccctacacag gaaggaattt
    38581 attatcactt ctctcagata tgcaagaaca cggatcttcc cataatggtc tataatatcc
    38641 cgcttttcac tgcttctaac attgatcctt ctacccttgc aaaactcacc gaatttgaga
    38701 atatcgtggc ggtcaaggat gaagcgggaa taaatcctct gcaaacctct gccttcttga
    38761 aggcaacgga gggaaagata gttgtctatt ccggcgatga tctgatggtt ctctctgtca
    38821 tgagccaggg aggaattggt gtgataagcg gaggatccca cgttatcggc gatatgatga
    38881 gaaatctgat cgaggaattc ctttcaggcg acgccacgaa agcaactggc agtttcaaag
    38941 agttgtattc gctgttcagc gcattctttg gaaggaacag gcgtttagta aatccattgc
    39001 cggcggtgaa gaaggccttt gcattacact ccggacttga tgtcgcacgt gtaaggccac
    39061 cattaatgga aatcgaagaa gacgaagtgg cagtattgaa agatacactc agaaagtttg
    39121 gaaaaatctc ctgactttca ggaacaaaaa accgaaaggg ggtttggcga tgaaaaaggc
    39181 atttatctta gttttccttc tgctcacagt gttcgtttgc tttgccgttg aggagaccta
    39241 tacaattcgc ttcaatacgg tggctgcacc gactcaaccg caggtccttg caatgaataa
    39301 gtttgccgaa gttgtcgaga atctaagtgg aggcaaaata gttgtcaagg tcttccattc
    39361 aggtcagctc ggagatcagc agacatcttt gctggcagta atgaggggag acctcgagat
    39421 ggccggtgat ggagcgccat cgtggttcgc cgatcttggg aatatgccta agatgggagt
    39481 gctcaatgca gcctacattt tcaagagcct tgaccacatg tattccgtca tgaacagccc
    39541 gatggtaaag ggatggttcg atgaactggc ctcgaacacc ggaatgaggg ttctggatac
    39601 atggtatctc ggtatgaggc agctcaacac aaccgaaaag gctggtcccg tttacaagcc
    39661 ggaagatctc aaaggcatga agatcagaat gccaaataac gaggccttca tggacatggg
    39721 acgcgcaatc ggaagcaatc ctactccaat gggcttcgga gaagtatatc tagcactcaa
    39781 gttaggtact atcgacggtc aagacaatcc gcttcccacc gatctctcgg caatgtttgt
    39841 tgaggtaacg aagtacataa tcctaacaga tcattctata ggaatgatca accctgtgat
    39901 taacgagaag ttctggcagt ctctgccttt cgagtacaag acttacatta ttaaagcgct
    39961 tgaagttgcc cgctactaca tgaactatct tgttctggag caggaatcaa agcttctgaa
    40021 gcagtttgta gatgaataca acatggagat aatcgtgccc gacaaagaag cctttatgaa
    40081 tctggcgagg gatttctact cagatgagaa gtttgacaaa gcctggggcg aaggaatgta
    40141 tgagacgatc cagtcgtatc ctctgaacta gaaagatgag ctagttaccc tggaccggga
    40201 agacaaccgg tccggggtat cgttttgggg gttgtcatga aaaagatatt cttgtcgctt
    40261 ttgaagacat tacaaattct gctgaatcta attgaatctt ggggcgggac tatttttctc
    40321 gggcttctgt tcctcagcat tttcttccag gtgattttga ggtatgtatt cagcagtccc
    40381 tccccggagc tattcgagat agcgcaatat agtttcatct ggacgatatt cctgggcgcc
    40441 ccttacgcaa gaaagttcga tgctcatata aagttcgaca tactcttcct taggttttcc
    40501 aggaaggtgc agctgatact ccagatagtg ttcgaccttt tcttctgcat agtccttctg
    40561 gtctctctgg ggccagtgct tggcgatgtg atcttttaca aaataataaa atcagaggtt
    40621 ctcagaatac cctggacata tctcttcatg tgctttccga ttttcatggt attgacgttc
    40681 attcacaacg tgatctggat agtgaagagc ttcctggaac tcatttccgg gaagacattt
    40741 gctaaggagg ttgaaccatg ggattgatac tcttttttat tatatttggt gcaaccttcg
    40801 ctctggggta tccgatagcc tttgggatga tcatgggggg ctttgcctac ttcattactt
    40861 ccggaatgga tctgggaatc ttcctagaca taatggcaat cgaactgagg gcccaggacg
    40921 ttctccttgc ggttcccatg ttcatatttg ccgcgaacgt tatgaatgat actgagatta
    40981 ctgacaggct ttttggcttt ttgaagcaac tcttgggagg tgtaaggggt ggacttgcgt
    41041 acgcaaacat tggcgccagc attatctttg cgggaatgag cggatcggaa atagctgatg
    41101 tttcaggtct cggaacaata gagataaaag cgatgatgga cgatggttat gacggtccat
    41161 tctcctgcgc ggtaacgtgc gcttcggcaa cgatttcgcc gctgatccct cccagtatcc
    41221 ccatggttat ctatgctatg ctgacaggga cttcgctcgg atacctattt ctggcgggaa
    41281 ttgttccggg gctctttctg ggttttatcg aggccggtat ggctttgtac ctctcaaaga
    41341 aaaggaacta tcccaaaggg cagagatacc cgctcaaggt aattgccaag agtttcatta
    41401 ggtcgttccc ggctttgctc gctccagtca ttttgcttgc cggaatctat ggcggcatat
    41461 tcacaccgac tgaatcggcg gcggtactgg ctgcatattc aattctcgtc agcatcttca
    41521 tttatcgcac gcttggactt aagaagctac gaaagataat gctaaagaca gtgtacaacg
    41581 ttggcgcaat aagcttcatg attgccggtg cctttgttgt gaaatatgtc ctagcaagag
    41641 aagagatacc gctgttgctc actaatgcat tcgtggatat gggtcttctg tcaagtgcct
    41701 gggtgcttct tttgagtgtt aatgttctct tcttcatcct tggaatgttc ttcgacgtat
    41761 cggtcataca gcttgtaata ataccgatcg tctttccact tgtgagggct gcagggatag
    41821 accccgttca tttcggaatc gtaaccactt tcaaccttat gcttgcgctg gatacgcctc
    41881 cttacggaca aaccggtttc ataactagtg ctatcagcaa aactcccgtc ggaaaggtct
    41941 tcaaagagat gctaaaatat tggataccga tagaagttat gggacttgta ataattactt
    42001 actggtccga tttcgttttg tggcttccaa gattgcttgg gtatgcgcat taaggtcaga
    42061 tcatagtcga ggggtttata tgcagagtgt tgaaaggatc gtcaaaattc tcagttcatt
    42121 cagtcttgaa gaatcgagac tgagtcttga tgatctgact aaaaagagcg gtttgcccaa
    42181 ggcaaccgtg tatcgaatcg cggaggccct ctgcaaagaa catgttcttg aaaaggatag
    42241 ccagactccc acatatagaa tagggatcaa gctttttgag ctaggaagtc tttatctctc
    42301 ttcaatgaaa ctgaaggatg tggcctttgc cgaaatggag aaactccacc ttcagactgg
    42361 agaaacgata cacatgggta tactcgacgg aacggaggtt gtctcgattg aaggcttcga
    42421 aagctttaag tcgctccaca ccaagttata cataggaaaa cgtgcgcctc tctactgtac
    42481 cgccgtgggg aaggcgatac ttgcctttct tcccgaggct gatcgcgaag gacttattga
    42541 cactcttgac cttattcccc agacttcgaa gacaataaca gacaaagcca agctcaggga
    42601 agaactggaa aggataagat caagagggac ggccttcgat aagggggaga atgaagacgg
    42661 tattgcatgt gtcggagctc caatatttga ttcatcgggc gtagttgtag cgtcgataag
    42721 catatccggt cctatcttcc ggatggaaga ggaagttatg aagaaatact ccgatctcct
    42781 caagaaggtt gtgtcaagga tctcttcagc acttgggcac ggattacagc aatgaggtaa
    42841 tgcatcaaaa taaaatctac tcgaaatcga gttgaaggag attaagaaca cctccctttg
    42901 agttagattt ggacttcatt atctgtatgt caaccagtga ttcttgagaa tgtgctatat
    42961 tactcttgag tatatataaa tctagggctt cgtgaacctt gcgaagctca tctttttttg
    43021 tacagtcaat ccagggagag ttaagcactc tttcataagg agtagcgaac ttgtcgtatt
    43081 tcttcactat ccttgtacca tctctggtct tttcgatcat cttttgagta ggctggaaat
    43141 ggttgttgta gagattcaag ctttcatata gctcattcaa gatactcagt tcctccaaag
    43201 tgtcgtatct gtaatagcca acggcccttc tgaccaaaga ccagttcttt tgttccacat
    43261 gaggattgtc gttcttgttg taactccggc ttctggtaaa gaccaatctt tcatccaaac
    43321 aatacttata caaatgagcg ttaatgaact ccgagccatt gtcggaatga attcccaaaa
    43381 gagggaaagg aagtaactct ctcaatgcaa tatagcttcg agagtccatc tttgagcttt
    43441 gttccttatc ggtgcaacga tactccaacc actggcaaca tccaccatat tcaggctata
    43501 acagaattct cccgagctat tacccctatc agaatcaatc attaacctga aataatagct
    43561 ggatagactg acgatccttt ctcttggtgg atttgataat cgctccggcg caacaaattg
    43621 atcgtcccga gtcttttgga ggtctcagtt ttccaatccg ttctgagttc gagcaaacgc
    43681 acctactaca atcttgattg ccaataattc ttcgttcgag cctccatagt atcagatatc
    43741 tgcagtgatc tattagagag ttttgtaaga ggcttgcttc atcaaaaaaa atggatgaaa
    43801 gtctaactaa acgcacatcg gttctctctt ccgaggtaga ttccacaaag aactgagcat
    43861 caactcctat ctttcttctc aacttaaaat atcttgtgat tgccttcatt ataagacttc
    43921 tacaaaagca tgcgtggacg ctgacgttct tctctttccc gtgagcattt atgaacagat
    43981 tggattgcaa ccactaatgg ataagggcaa ccaatgactg aatgactgtt gataatgatt
    44041 ttttcacaaa tgctcgggaa tataatacgt ttgagagaag accatgaacc gagcaacata
    44101 aatatgtatt tcggaggttt ttctttgaga acctacagga atagaaaact caggtttcgg
    44161 aaacttatga tcttcgttgc agcaatttct tttatcttcc tatctactga gtgtatcttc
    44221 aagaccgtaa ctctaactat gctcgagcca gatgagggag gcgaggttca gccccttccc
    44281 gggaatcata aatacttgat caacacgtct gtagatattc aggccatgcc cgatgatggg
    44341 tgggacttcg accgatggga agtagatgag gaattctatt caaatgagaa agagacgaaa
    44401 ctggaaatgg attccgaaaa gacaatcaaa gcttttttct cgagacaacc ggttacctta
    44461 actatggaag atgaagaagg agagggaagt atttcaccgg taccgggaga gtatcagttt
    44521 gattattcta ccctggtcgg tctttcagct actccgacag aaggctggga attcgaccga
    44581 tggcaggttg acggcgtttt ctattcgagt gaagaagaga cggaattgtc tatggacgca
    44641 gataagatag tagaggcgtt ctttaacgag gagcctgtgg cttcggtcac tctgtctatg
    44701 cacgaaccaa ttggtcaggg agcggttgct ccgactgtcg gagatcacaa gtatgatgca
    44761 ggaaagtctt taacacttca agcgacacc
//
LOCUS       DAOQYI010000015        41245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1068_length_41245_cov_75.250716, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000015 DAOQYI010000000
VERSION     DAOQYI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 41245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41245
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1068_length_41245_cov_75.250716"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKL93_05845"
     CDS             complement(<1..502)
                     /locus_tag="PKL93_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ70820.1"
                     /translation="MATTLDDIAKETGLSRATVARAIGKYGYVSKKAREKVIAAAKKL
                     NYKPNYIAKSMATGETKNIGLIVGDIQNPFFSTIARAISDVIVPEGYSLIVTSTDEKV
                     EVEKTSIDRFFQKQVDGLILAPVSRSNSDHLKDLVKSEIPIVLIDRIIEGLDVDMIVS
                     DDLGGGF"
     gene            753..1451
                     /gene="deoC"
                     /locus_tag="PKL93_05850"
     CDS             753..1451
                     /gene="deoC"
                     /locus_tag="PKL93_05850"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNQ70821.1"
                     /translation="MERIDKFSKRFDSTLLKATATIKESEVFIDDSVSCDFRAIVVPW
                     YLMDRVVKKVRGTDISAACGSGFPFGFETTETKAYMIRESLRLGPEVRDIDITANISA
                     MKSGDWDYFEREVSYLSGLLKDVTCKVIIEVSYLDTVEIEKACSILMKLPHVDFVKTG
                     TGYGSRPTTLEDVRIIKRVVGDEKGIKVSGGVKSLSQVEDFIAAGADIFGSSNAVAIY
                     REFTEKYPGRTDLA"
     gene            1474..2142
                     /locus_tag="PKL93_05855"
     CDS             1474..2142
                     /locus_tag="PKL93_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNQ70822.1"
                     /translation="MRLFIDDGNVQAISELFDLGFFSGISTNPSILKRTGRKPLEVIK
                     DMLNRFTGSLFVQCASRGHDEVLKEGQELFGLDPERIILKIPFSRTGIRVLREFKKMN
                     INTLITGVFSVPQVLMSAEAGADYVAPYANRIENLGIDLSVVGSMQKVLSNGDYETKL
                     IAASFKTLTQISKMAELPIYGMSLPVGMYNEFFSHSGTLKAIDNFEADWDSIEDREWV
                     PLKS"
     gene            2175..3695
                     /locus_tag="PKL93_05860"
     CDS             2175..3695
                     /locus_tag="PKL93_05860"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNQ70823.1"
                     /translation="MKYIMAHDLGTTGDKATLFSQTGSLVASSFAGYETFYPGPGMVE
                     QDPEAWWTAFCKTTSEIVQMAEIDTKNISALSISGQMMAVVPVDRSGELLRRSIIWAD
                     SRSTEQVEELLAKVPASDLYKITGSRLSPTYQGLKIAWLKKYEPDLYERTFKFLQAKD
                     YINLRLTGRFATDFSDAVMTALFDISSLTWSEELISALGIEGSKLPDVYASITDLGEI
                     RKEVATDIGLPNSCRVILGAGDGCSAAVGAGAVEVGDTYLYLGSSAWISTITEEPLLD
                     KEMRVFSGAHAVRGLYFPSGTMQAAGTSYSWIKDTMYCDGSMKESDRDIYSYLDTQLC
                     QSHRHSESILYLPYLLGERSPMWNSNARGTFIGLSASHKKVDIVRAVIEGVSMNLKWI
                     LESLEESLPIGKIRVIGGVIKSRIWKRIISNILGKTITIPENVDEATSIGAAMIAGVG
                     AGLFNFSAVRNFVVDVEEIEPIGEQQEDYLRLYELFKESYLALEKTFEGLNGVRRG"
     gene            complement(4349..5365)
                     /locus_tag="PKL93_05865"
     CDS             complement(4349..5365)
                     /locus_tag="PKL93_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HNQ70824.1"
                     /translation="MDVRSFFFRLKKNGIVISGRSMELLYPLNSKVVFFNRGDSFSSH
                     MPDGSEAASWENKYGFVGLNAFDIPTAISDGLNEMGLSLSALWLPGTEYEQVSTESDP
                     TNVIELFDLPSWILGNFDTLESVREALESMAIWGEENELLGEVPPLHLSLHDKFGGSM
                     VVEFIGGEMKIYDNPNAVLTNAPELSSHLENLRNYVNLSPYMKEGEINGITYKEMGFG
                     SGYVGIPGDGTPPSRFVRIALLREFSNPVETEEEGVMLALHLLNTVDIPSGVSKREGD
                     GPASFENTQWVTIKDNKNLKLYFRSYDCSSLFLVDLNKVDFSNGSEHESIIVDKEFSV
                     IDAF"
     gene            complement(5712..6248)
                     /locus_tag="PKL93_05870"
     CDS             complement(5712..6248)
                     /locus_tag="PKL93_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNQ70825.1"
                     /translation="MAEAKKFDWISLLVGFVLIIGGIFSLSNPLATFLTLAIMLGIVV
                     LVRGIMLIVAFFKLENRTGAKVFFALILGILLAITGVVFLFRPLFAANVFAFIIAIWF
                     IVDAINNLINADRLKPAGKGIYFLSIVLNVLVLVGGIILVLHPLIAGLSVAVMIGITL
                     LVFGVNHIVLAFMGKESA"
     gene            complement(6296..8668)
                     /locus_tag="PKL93_05875"
     CDS             complement(6296..8668)
                     /locus_tag="PKL93_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNQ70826.1"
                     /translation="MRKVPLVILIISILFCSHILATKIMVVQSYDNDYSWSRELLDGI
                     QTVLSDPSYEWKVFYMDTRLQTSEEWKIKSGVLASEEASAFNPDILIACDDNAMEYFA
                     KDYHDAPVVFCGVNLDPSIHYSANANQTGVIETIFANGTISMILELFPEIQAVRFIGD
                     DSETFEGMKEQIQRLDFGDTSVEIDGFKVFEDWKNYIEGVSPREAVVLLSVRTIEDGS
                     GNTIGAHDITQWTVENSSGPVFGVSDFIVRDGAVGGVVNSGFEEGRQVAILASRILSG
                     SSASEIEIETSKNPKTLLNTEALSMWNIELDVGWTDIVDEVITDWSIPAQSILNLIMT
                     SFEERLLGIISSLRILASCAEVKSGDWSLIRPLLEKFNINYEGLALYILPDGGYYTVQ
                     RDWTGLNLSNRGYFPDLLEGKEVLGYQVLSRSTGKRSVVFAVPVMDNGKISAFVGLSA
                     FFENWNFSLIERLVLLPDIHFYVFDVQNSLAMTDDTALLLGSLDYPVVGLSERLGGLS
                     ADDGSLIYSDGESLNTAYFKKSTQCGWTFVISKPISDVAEEKDMDLILNSVQKSIQEQ
                     FNLMDSSLAKGAEEIRSVLQDPSGIRKILKDLYSNNPQVVNVSFIDIEGVLKYIYPDE
                     FDYVEGDWIGDQEQVVELHRRGLPVLSQSFVAVEGFPALDLEWPVFTKEGELSGSLSL
                     LIKPETFLAPLIIPKNYEPYEFWVMQPDGLIFYDQDEMEMGRNLFTDQLYEPFEELRV
                     LGIEISQKESGWGEYRFFSRGMDTVVVKEVKWSSVGLHGTQWRLILTKSN"
     gene            complement(8821..11046)
                     /locus_tag="PKL93_05880"
     CDS             complement(8821..11046)
                     /locus_tag="PKL93_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNQ70827.1"
                     /translation="MKRIGVVLLFALLCAIIYAREFRSIEEIKKSGVLRILQTEDYWY
                     PFYYLDSDGNLSGIDIELGRHIASQLGVEAEFIRTASGHETLAENLSTGEGDIILSYY
                     SYSPRRASQIFLSRAYLTERLSVLVNTQLFEEIKKNDPEMSVEEAFETLNRPVVSIMT
                     VADTVYESLAKQYFPNARVTAVDSTEEASRRLVQRTEEAFFLNNLWTESFLVRNTSLL
                     LHYSLYLLPNEDQIVIGVSQNNPELLEWINRFLSSEYNRTEEFVSKYIDFDLVSKLYD
                     PVDKKEEILLVGDPYRWIILVLLIVFSIVVVHNSQNKLRRNKSPEDIPHWLLNIWTIL
                     FSMVLGFYVGGAFPEIIDFLSPIGTLFFNYLLFCGLPILFCVVTLNFLKLIERSDGAR
                     FIKRFAMVMIFFFVIGAAIGLLTALVLKPGVGIPIEAQEALVSSMRETDPYEEVELSA
                     GSIIWALPSNMIGNSVVKAFSENRTLTIVFFSIIFAFAVRYIQSSKRTGIVNSVETIN
                     DAFLFLFRLSYYVLPIGIFSLMLGQASIFIRDTEVLIALVMLIVCEIVCFIIWFLIGT
                     LLGARKASLSPKKYLGVIKKPMMLLFALLSSVAVIPEAVKTCEKSEQFNDENQKGVIP
                     LLLVMFQPQTVSLFVVVAIFNMQLLGKSLNAGDYLYIMFSSIIAVFASIGVPSPLDLF
                     TISMVVLPFNIPPAQVIFFLLPWMYTGRRLDTLAMATNDFAVVQFFRKKQTKNSDSEA
                     L"
     gene            complement(11141..12673)
                     /locus_tag="PKL93_05885"
     CDS             complement(11141..12673)
                     /locus_tag="PKL93_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ70828.1"
                     /translation="MKFDCIVIGAGIAGLSCAAFLSKAGKKVALVERAEKPGGCFSSF
                     QYRGYHFDSGIKAVENAGLLFPMLENFGIMDKLDFVKNRVYAGFEDKVFSMESDESLL
                     AYFDFLGERFPESRSGIEDLLKEMEELNSFMGSLSGMSGPLYGKTTFLTKLKSIPWFF
                     KNGKKLLGFLKKLKYNNVPFRDFLRARILDDELVSIIDEPFFDGTPAFFGLAYPTVFR
                     DYFYPKGGIERIPLEIAEFVRNTGGTVLTNTEVTKIILERNLPVGVVTDTGDELKASS
                     IVNAADAKRLYNRLLPENTLDKDFLQRLNRAQLSDSVFSVFMGVDTPPENLDKPVEHI
                     IYVPVAAKYCDYESPDYFKTAGMEISIPCLRDPSLAPEGKTGIILNLNTTQKAMNGWG
                     KGISVEEYERTKEEVADDVMANFFRLYPSLKEKVEFRIVATPFTMEEKTLNSGGSITG
                     WNYHPSETFPLRDFMKMKKSINTPVKNLFQVGQWSFDPAGLPVCLITAKIAADRVEKA
                     LG"
     gene            complement(12670..14562)
                     /locus_tag="PKL93_05890"
     CDS             complement(12670..14562)
                     /locus_tag="PKL93_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ70829.1"
                     /translation="MNGKFFPESKYDVAVIGSGLGSLSAASLLANKGLKVLVAEQHYL
                     PGGCCSIFRRNDFTFDSAVGMVFGFGNRGFNSHRFVFNEIGEDIDMVRHEALYTVIFG
                     EKRIVFWPDLDRFIEELGNNFPGYEEQLRKFYNYLADLYHNVIVADPVIVPPTEIKRS
                     DSLRSLLKHPVRQAKMIRLLFKSTEELMSSFIDPEAKELYQFFDVLTSTYCYTTVKET
                     PGVLAVTMFIDNHEGGGFYPVGSSQMIRNKLEKAIEKNGGYMLYENRVNEILFDDGKA
                     TGIRLEDGSEIRSDFVIYGGTVWNLYGGIVPKEYVSQESVKWAGSLEPTFPAMVVYAA
                     VDSSAIPAGTNPVEMFVEEFDEIAESDVTVYIPSIDEPSICPEGTHVMSMIAPSKKRW
                     PPRGTKEYREMKEDETERVIKLIEKRFPKLRESLKHLETATPLTIERYTLKNEGRVGG
                     PKQSIGQELLKRLHAKSEWENLFICGDSTVMGMGTPAVTVSGIGAANLVLRKLKMKEY
                     SYREPEVERVHYTAGKKKVPVDLENTVLDRETVPLISSSCQLCDIPNCTLKCPDGKDI
                     RGILRRLEAGNFIGAKKRLLETTIGTLCSDCAGFCEKSCKRLSFDKKPVPIKRLLSWL
                     EKEVIV"
     gene            complement(14748..15608)
                     /locus_tag="PKL93_05895"
     CDS             complement(14748..15608)
                     /locus_tag="PKL93_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNQ70830.1"
                     /translation="MKKVFLVLVLIGLVFTGFSKNIVEVAVSAGNFTTLVAAVEKAGL
                     VETLIGDGPFTVFAPTDEAFAKLPEGTVESLLNDIPALTRILTYHVVPGKYMSSDVAS
                     LESLKSVEGSSIPIKVEEGKVYVSNAMITAVDIEASNGVIHVIDTVIFPPERETRRIP
                     EIAINAGSFTTLVKALQEAGLVEALMGDGPFTVFAPNDEAFAKLPEGTIESLLKDIPT
                     LKNILLYHVVSGEYMAEDVLGMRSLTALNGGQLMINPTEVKIQGSGIIVTDIIAANGV
                     IHVIDTVMIP"
     gene            complement(15916..17130)
                     /locus_tag="PKL93_05900"
     CDS             complement(15916..17130)
                     /locus_tag="PKL93_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNQ70831.1"
                     /translation="MLRRVHSKHYGHEWNVAAAVLRGCNTKELLKEYFSVMGRRFGIF
                     FEVFPYGKRMHEATDLDSFLDSAIEDMKKDEWLIQNSDTFELTERGEFEARKMLAELE
                     NSGRLLEKATRAETGSRVTIVVHFILAALKLPTAILSGSVGLLNDSFDTLLDGISSVF
                     VYWGVKKNHEHLVSLILLLFMGVTGVFSLIEALFRIISGEIPSPDLLTFTAVAISGIV
                     CALLWFYQKYSGLKNRSFPLITQSADSRNHVLVAVSVAIGLIVSLMRIPYADAIVGLI
                     VSILILKGAAELLIDLIRSARGEAIDFERYGFSLFNKFRAKQLKHWFLFMIDQGNIQT
                     RDQLECEAKASMAYQDIEPLRALGISGSQSDESIVKTALEALDKEMLITEYDGHLKLT
                     EKGSYELRSTRM"
     gene            17532..17762
                     /locus_tag="PKL93_05905"
     CDS             17532..17762
                     /locus_tag="PKL93_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70832.1"
                     /translation="MALITSIIIPPRIESIDWRVIAELLSLMIVVQALKEYKVLEKIA
                     TISLSNLRSARSIGSVIVIGCLEKKGLQKIQW"
     gene            17805..18182
                     /locus_tag="PKL93_05910"
     CDS             17805..18182
                     /locus_tag="PKL93_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70833.1"
                     /translation="MLSQGMEKKEIIKVLDNLDKFIVKGTVKKGRGRNKEYDEITILK
                     LLVLLTLAKISYRGAKGFLEGNGEYIEYLGLKTIPSFQTLSRRARQLPFHEIVQKATT
                     LFTDAEGLERKEVAVDSFREQVM"
     gene            18166..18768
                     /locus_tag="PKL93_05915"
     CDS             18166..18768
                     /locus_tag="PKL93_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ70834.1"
                     /translation="MSKSCKGSTAQRRRKRGKYKDQGASWTKTNKGWQYGRKVHISLD
                     LVNKLIIDWIITGGSTHDSKVAPKMADTCKGFKYILADSAYDSNQFYGYIFEKTGLIP
                     VIDINRRRGLDTEKQKQFRWLAIGLKKLHSLRYQLREEIERTNSILEGILDSEFIWYT
                     RNRDFDTAIGLKILTYNLVVIYNHLHHRSLRKIMDIIHAI"
     gene            18795..19535
                     /locus_tag="PKL93_05920"
     CDS             18795..19535
                     /locus_tag="PKL93_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNQ70835.1"
                     /translation="MLTAFMGALITNDVALIALVPLTMTIAEKAKFEPVWIVIIQSQA
                     ANIGSALTPIGNPQNLFLFEKYEIGIWEFGSLMPPFVVFGVCWTLVMNLLNTKRKIVF
                     DVPSSEIREPQKIDYFLLGTFLLFFVFIDNISRMEIIATMMKSATNGEIRTMTSSALL
                     SQFISNVPAAILFSSFTGSYKELLLGVNIGGSGKIIASLANLIAYRIYVKEKGRTLKY
                     IAVFTASSSITLILSIVFGYLRLKLTGL"
     gene            complement(19532..20626)
                     /locus_tag="PKL93_05925"
     CDS             complement(19532..20626)
                     /locus_tag="PKL93_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70836.1"
                     /translation="MTKRIISMLSVAFFALSFVIASSVWLAIFPEYSDSPLEWMAPGL
                     RLSFRASTSVMKPGGGLSDTGGSGVGLYQMDMVAVEQGSLALLQTYFMEMKGGYYPSV
                     SFGTIEKPGVSDMWLNPKVFDDLSRFNSDNTAAVEMPIQYGGQTVQAVRIESRGVNTK
                     HVYTYDRASGILLYLLTQAPSGTDIHQNILEFLSARYVELPWFNSQRPAWKLTTTSYS
                     GTYTINIPGSYTTPTQMQVKITPTTSSLSWDYFKMTISQYGSIPRDNYNVTGVAQLNG
                     PIWLPEKALDSLNKLQSIDQDPITNVVTSVSYIGELQDGTEAIMLQQTNGTYANQHVY
                     DRKTGRLLYTKQMSPNSLDYNITELSIVGY"
     gene            complement(21120..22097)
                     /locus_tag="PKL93_05930"
     CDS             complement(21120..22097)
                     /locus_tag="PKL93_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70837.1"
                     /translation="MISKENMASVVRRYGVLFALIAVFAVFSFMEPTFYSVRNILSII
                     RQASITGILAIGLTTVVIAGEFDMSFASLAGFSGVLSLILMGKVFMGAIPAWILSVLA
                     ALGIGALNGLIILFIGVPSIITTIGMMTLLAGFTKWLTGGSTYYAAKFPDLFPFLGRS
                     FLFEIIPMPVVIFGIVITVFVVLLEHTKIGRFFHAVGGNPKASEHVGIKVRRVKFIAL
                     LISGFMAGLAGIMIGSLLGSGAPGMGDGYLMPGISAMFIGAVFLRDGVPNVWGTVIGS
                     LLLSVLTNGFVMINLPFYLKEIVLGSVLIGAVSMVAIFKKGSIPGVNLL"
     gene            complement(22163..23662)
                     /locus_tag="PKL93_05935"
     CDS             complement(22163..23662)
                     /locus_tag="PKL93_05935"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNQ70838.1"
                     /translation="MSDKSIVLKTVSISKSFPGVKALNNVDFDLFEGEIHAICGENGA
                     GKSTFCNVLTGIVRPDDGKTFLNGEEIRLTHPSQALKMGIRMVYQERNLIPYLTGAQN
                     ILLREEPVRGGFLIDERSILKKAEKIAALYDLKVPLDVPVSLLSAAQRQAIEILRAFL
                     YKPRILILDEPTSSLTESEVQSLFKAIHQIKDEGVSVILITHKLEEVFENADRISIFR
                     NGERIITRDKEDLSEEEAIRLMVNRNISSLFPEVTNRAGDTILQVENLSGSGFLRDIS
                     IYLRKGEVLGFYGLIGSGRTEFIEHLYGLRETKTGRVIVKGKEVHPNVHKMIDEGVFL
                     VPDDRREKGLLASLNLKKNLTISYINEFSKLLGLVSARKVNSLVKTILDKSSLSLKYS
                     NINQDVQVLSGGNQQKVVIGRWISKDHMSVLILDDPTNGIDVGTKFEIYKMIRNLAGE
                     KGVGVIFISSELPELIGVCDRLYIFKEGKIAGELHREEFDDEKILSKAI"
     gene            complement(23746..24807)
                     /locus_tag="PKL93_05940"
     CDS             complement(23746..24807)
                     /locus_tag="PKL93_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ70839.1"
                     /translation="MKKRFIISILVIMVLTLSGMTFAGTEYAKDVTVAICGHGLEAES
                     CQRDFQQVKIECAHRDWEFIDAMTVRDAAQQRETMETLIQKNVDAIVIIYWDMEAIAD
                     LILKAAEQGIGVYCVDTELKPGVIVSCTQNNAVAGALMTYWGIQRLNERGKVAIMSGK
                     WHIMRRRAYVAATLFERDWPAVELVAEEFLPLVGYEESAFNVAVNWVTKYGNDLDWIF
                     AGWDTPGQYAARAIEQAGFDKDDIFVTGIDGGSSVYSMIRSGSPFVATVSQPFEYYVH
                     TVFEVINQVETGIGPDHPDSLVPPGRVIYATPYLTDETNVPEPGTSIHAVFGYYGMDP
                     DDPDAWFNWQDAGGPYKIQ"
     gene            complement(24822..26483)
                     /gene="dhaL"
                     /locus_tag="PKL93_05945"
     CDS             complement(24822..26483)
                     /gene="dhaL"
                     /locus_tag="PKL93_05945"
                     /inference="COORDINATES: protein motif:HMM:NF011049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HNQ70840.1"
                     /translation="MKKIINSTDNLITEMLDGIVMAFPKDFKRVEGFDKALITPRAPV
                     KNKVGIATGGGSGHLPLFLGYIGENLLDGAAVGDVFSSPSAETMLAVTKAIDSGKGVL
                     YLYGNYGGDIMNFDMAAEMAEEEGIRVETSIGCDDVASAPADDTGSRRGIAGLIFAYK
                     IAGAKAATGASLEEVKETTDRALSVIRTMGVALSPCTIPAVGLPTFEIGDDEMEIGMG
                     IHGERGIKREKLKSAHEIASFMTERVIDDLPFESGDEVAVLINGLGATAPQELYVTFR
                     KVKEILDLKKINIYKSFVGEYATSMEMKGLSITLFKLDKEFKELLYAGSFADASAAIL
                     RDGGQMKEAISAAKISEILYGACNSLEKNYIDEFRALDAAIGDGDLGITIRKGCTAII
                     ETLKTFQTEDTGALIKKCGIQFNQANPSTFGVLTASAFIEGGKKVLGKESLTLDEIAG
                     MFRAALEGIMKRGKAKTGEKTMLDALEPAVEALEKAASEGKSIKEAFEKAAEAAKDGL
                     ERTKNMEAKTGRAKAFGSRTVGEQDPGATVVYRFLREVSEELHKS"
     gene            complement(26486..27235)
                     /locus_tag="PKL93_05950"
     CDS             complement(26486..27235)
                     /locus_tag="PKL93_05950"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HNQ70841.1"
                     /translation="MLKVFYCGDQVMLITTYVKGMNSWTDGVVHDESFHLRKALESDS
                     EIDLTYLPTSEVQRNFPKPDELGQYDVILFSDVGTDTVIMYEDRFNNCPMGVDRLGAL
                     KKWTRDGGGFCGVGGWLSFGGMFGVAKWHKTPVEEILPVTCYPYDDRVEISEGMTPKV
                     LKKDHPIFKGVDFDSAPPLFSGYNEVKAKPEGEVLAEHNGDPIIVTGQYGKGRTLAFA
                     TDPAPHWGRGMVEWKDYGQFWVNVVKWLGGK"
     gene            complement(27526..28641)
                     /locus_tag="PKL93_05955"
     CDS             complement(27526..28641)
                     /locus_tag="PKL93_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70842.1"
                     /translation="MFSSILRLCRFLLLIFMFIGSFCGAFWVDSYLDNISTTGVKRIE
                     IKNYNTQDGELWIKFATLTAEYDESGNTIRESKVAGDGVLEFEYSYQYDIEGNMITMQ
                     GKRIVEDSVLEHSYEYKYDENGRQIEGIQYSTDGSITSKYSAKYDEGGNFVEGNDVQY
                     DSTTSTRYLASYNELGYMTEETKYIVYEYKEEEGLQTEFRNEYQYDDLGNLLLEIGYG
                     KYGDFSYKYGYEYDEEGNLIKAYNYNASDTLISFYEASYDDEGNLIRFILSDANGKIL
                     SKHEAQYENGNMVLEVDCLSESSNPVYSAEYNSEGLKLKETNYNRNIGGSPLTYSYEY
                     LYDERGNCIQERYLVYFEDEDLWKPITKTVYLITYYE"
     gene            complement(28671..29312)
                     /gene="dhaL"
                     /locus_tag="PKL93_05960"
     CDS             complement(28671..29312)
                     /gene="dhaL"
                     /locus_tag="PKL93_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HNQ70843.1"
                     /translation="MTVLSKDEFIEIVGFAVDSILENEQYFCDLDSVAGDGDFGASLA
                     KGFSEVKSKWQDLEKSNVGTFLRDCSMIIMEKCGGASGPIWGNAFLSASRASKGKESL
                     EIADIAELFQAMVDGVQKVGKAELGDKTLLDALIPFKESLKESAKLNEPIEEAFSKAV
                     TRAEEGAESTKTIVATKGRARYLGDRSIGAYDAGARAVAVIFSDIQNRFFKKT"
     gene            complement(29316..30308)
                     /gene="dhaK"
                     /locus_tag="PKL93_05965"
     CDS             complement(29316..30308)
                     /gene="dhaK"
                     /locus_tag="PKL93_05965"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HNQ70844.1"
                     /translation="MKKLINDPKSVVIEMCQGMVKAFPENLALDEEFMVISRKNPDKN
                     KVNLISGGGSGHEPAHAGFVGKGMLDAAVCGDVFASPSVMQVFNAIKHNHSEQGVLLV
                     IKNYSGDVMNFESAAAMAEDYDIRVDSVCVNDDISIEKKEDRRGVAGTIFVHKIAGAL
                     AERGASLRQVKEIAQRVIDNVRTMGIALTSCTVPARGKPTFDLEEDKIEVGVGIHGEA
                     GIFRERLLSAKETAELIIERILGELSVSKEEEVAVLINGFGGTPLQELFILNNEVASL
                     LTNKGITVAKTLVGNYMTSIDMEGGSISVLKLDEQMKRLLSAPCETAAWKETEA"
     gene            complement(30333..31877)
                     /locus_tag="PKL93_05970"
     CDS             complement(30333..31877)
                     /locus_tag="PKL93_05970"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNQ70845.1"
                     /translation="MDENIKGNNKILFEIKGIERSYPGVQALKDVTFNINRNDVIGFA
                     GENGAGKSTLLKLIAGVESPDKGEMFLEGKKYAPQSFREANTLGVSMVFQEQNLVQSL
                     TVYENLFLSHEKRFQVAGTLKKRQMISEAKRYLESFGLDIDPRKEVSRYSFHERQMLE
                     IIRAFVIADMYKIKTPLILLDEPTAALPEKEREILLDRIKSFSNKATFIFVSHRLSEL
                     MNACNGILILKDGQLVGEVDPEVSEEREIHPLMVGRELSADTYRVEDQKEFNGDEDPV
                     LRVRNLSKKGLYDSVSLEVRRGEILGIGGLIGSGKKFLGETLFGVGEPTEGEIIIDGH
                     SVPKTTIRNMTKLRVGYVPSERKENGIIGLSTVANNLTITRFSEFSSRLGFINNRQES
                     KLIKEGIEKFRIKATSEDLCFSLSGGNQQKIVLTKWIMKNLDILILDNPTRGIDVGAK
                     EEIYTFIREAANKNLAIILITDDLLELIGLSNRIVIMKDGNISLIVDADKRRKPTEQE
                     LVRDMV"
     gene            complement(31923..32882)
                     /locus_tag="PKL93_05975"
     CDS             complement(31923..32882)
                     /locus_tag="PKL93_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70846.1"
                     /translation="MMDNRNGKRKFPLSKLWTPLLLVVLFLIFGLQYPTFLSVKNFNN
                     LLVQMAILLIASMGMSMVVLMGSIDVSVGATITITAVASAMVSSALGGASIIVGMIVG
                     ALVGLINGLLATFLKIPTFLSTIAMMGILNGTAVLILKGSPYNVHSESFLAVARGSFL
                     GGISNLITIPIIVVLITIIITKKTVLGRMIYAIGNNEYVCQYSGISVVKIQTFVFMIH
                     GSLVGLAGALDASMLEAACPYCGDTYTLPIIAIVVMGGIPLSGGRGGPIGTVLGAMLV
                     TVLISGLNFMGVTPEFRNVAMGALIIAGALMSNVGRPKIITVK"
     gene            complement(32898..33881)
                     /locus_tag="PKL93_05980"
     CDS             complement(32898..33881)
                     /locus_tag="PKL93_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70847.1"
                     /translation="MRNLALKNLLKKGLKTENLIEVLPYLLLVGIFVFFAISYPPFAR
                     IRNITGILTVSSVFLLVAAGETFPILMGSIDLSVGAMLSSSAIVAAYFFPQGGLGMIV
                     LAIVVGFLSGLVNGLLVVLLKLPSFIVTLAMMYVYVGIATGLTNGYNISIRNRDFAWI
                     STGQMIEGIPNVIIWSLIVFAVFVFISRKTVVGRYILAIGSNEESVKKMGINTNKYRI
                     IAFMFSGLLVGIASVLLSSYLGMAPSRLGDKYLFNTLIAVVVGGTSIMGGSGGLMKTL
                     IGVLLISMFDNGMSLIGSTSNVQNIAKGILLIAAISLVMLPHRRGRLLLTK"
     gene            complement(33968..35320)
                     /locus_tag="PKL93_05985"
     CDS             complement(33968..35320)
                     /locus_tag="PKL93_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ70848.1"
                     /translation="MKKLSVLLIVVIALFGVTALSTETMVCTYSQMANDFFLTFDKGA
                     REAVEALGNMYIAASDDRSPEKFLFQIESFAAAGVKMIFGYSPTIESVIQASNFVNRE
                     KVYYANILEIADWWTPLDAGPYYGQFIGPDAYVDGYLGGLEMGRLLNGEGETCILWGF
                     PGSKAGNDRARGYKSALMENFPNITVIGEEYADFIRDKGYSVTKDWIAKFGDRIDGIF
                     GTATSMNVGAAIACEEAGLDHVFQVTTDANKSNLVDIQDGSLDVVAGIYGFWMAGRQA
                     VTVYDMKNGWKPTIPETMMSTMQTFITPENVDWYIETFGQEGPYDYDWKMMSRTLHPN
                     DWDPQGLLVPIYPERFTNWIYLDKPRGYEVPKEYKDAMESGLYQEIYELYLDKFTAAW
                     VVPPFESSPYNYVFVANNLEEGLRADIVKRMENDVLVHEVIDVPYRFDIPGFGEGCNN
                     "
     gene            complement(35418..36056)
                     /locus_tag="PKL93_05990"
     CDS             complement(35418..36056)
                     /locus_tag="PKL93_05990"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HNQ70849.1"
                     /translation="MAVVDVKLEKLSEVSMRDFSTFGRIIGRDEDTEEGKYELHPVDA
                     KAAVENSMIKAYWDLVPFKDNKEDRFSMGMLFVYPKPIGEILNWTEVHYETLEFFFPL
                     GGKQFIFVLAPKSKIPEAEKTRAFLIGPNEGVLLNKGTWHYPPFAIDGPTPVLMPRFG
                     KLGEITGDVTEAYGKKWETPVGNRYFKGQLHALKTDYYGEGYGGEYKIRIIL"
     gene            complement(36390..36983)
                     /locus_tag="PKL93_05995"
     CDS             complement(36390..36983)
                     /locus_tag="PKL93_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ70850.1"
                     /translation="MDNSLNILIAEDEYIVLRGLKETLIGLGHKVIGEAMDGETFVKL
                     ALETDPDLLIVDVNLPALSGIEAIERIGKVKLVPSIIVTGYSSEETVKRANSAGVFNY
                     LVKPVDEKELKPAIEIAMGRFKEFMLLKKELHEQEEALEARKLIERAKGILMDRNGIR
                     ESEAMKLLQKISRDRNLKLVAVAKEIIEADNALHYGI"
     gene            complement(37037..38899)
                     /locus_tag="PKL93_06000"
     CDS             complement(37037..38899)
                     /locus_tag="PKL93_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNQ70851.1"
                     /translation="MEQCILDIKELSSNDTGNVDLKEINLEVTSSEVHALVGEYGAGK
                     ELVVNVLTGAEKKISGRVFFDGIDVTNKIKIGGSTDVLFLLEEPMVAEGLTVAENMYV
                     LRSHSNVIKLINHSKINRAVKELIARFELGISEKTHVYKLSTEEKKLIELLRLCIYEP
                     RLVVLYEPTANLGSKSQDLLLRILNELKGKGTGILYVTNKWDEALKYSDRISVLSEGV
                     LCGTLSSEEAKDKPEKLLKLILGWKKSDSTKREAEEIETEDIIDAVLRAAEYLTSNYE
                     INDVLKMLENYTCKIMESDSCYVYLVDEKTRTIMDTIKHKETESIEAVLNDEVVMRIM
                     DNKKLFVSNFDDVLFEKLFKSIRNTETIVCDPVSVRSHHTALFQVGYRKYYEPNEKQK
                     KIFHTLSRQVALAIDNTRLMGSSVLLQESHHRIKNNLQTIVSLINIQKRSFEKKTDNE
                     VEDLLDNVVSRVKSIAAVHDLLSHDELGRSIINLKSLVDTIVRFNIVDGTLQVRKDLD
                     DIFIPYDKASGISLTISELINNCVKHAFKDIDSGLISISCKSFDDNVEIVVEDNGAGF
                     PNRSFSAKDEKLGLSLARSIVENQFDGTLEIASERGAKVKILIPKESLLLTRGS"
     gene            39353..39565
                     /locus_tag="PKL93_06005"
     CDS             39353..39565
                     /locus_tag="PKL93_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70852.1"
                     /translation="MSYKHYYVGDSVLSTGDYEVHTGDCVELARVESKTYLGYYDNCQ
                     DAIRKASDIYPNVSGCKYCSPGCHRR"
     gene            39730..40974
                     /locus_tag="PKL93_06010"
     CDS             39730..40974
                     /locus_tag="PKL93_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70853.1"
                     /translation="MNDGRTKVGIGFATGRRHFQRVLRSYVYNWKESELVDSRELTLN
                     LFVAYDLNYKNTKKANYTKISSGVDELLFSKHFIGQKEVEEEKRELIKRGIVDSEEAS
                     LVFGKGYASQRNIVLYSALKNHIDCLLYLDDDEYPVAVTNDMGSVSWMGQHLLKTHLN
                     YIDQADITYGYHCGYISPIPFIEFSPALPEDTFRLFIEAISNDIVKWETLKRLIVAGG
                     VSYADPKILNSGEVFEVEEVNHAKFISGSNLCLNLKDPGRLFPFYNPPGARGEDTFLS
                     TCLSDRRVLKVPSHAFHNAFSTYNHILMGVLPTSLRPIRADSRDIVRRFYRSCIGWIR
                     YKPLLLYITDRNNYSKKIEEMKINLRETLPSISSYFGTDSFYNISKELNKYHKSVEKH
                     YEQFERVKEIWSKISDYLREAH"
     gene            complement(41031..>41245)
                     /locus_tag="PKL93_06015"
     CDS             complement(41031..>41245)
                     /locus_tag="PKL93_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70854.1"
                     /translation="ILDDDWRYSMIRTDKCPNCGSLDIGKGYWSGYAALMPLGKPLSM
                     GSKVIVRVCRECGHIFDLQAEKPDKF"
BASE COUNT        11344 a         9679 c         8412 g        11810 t
ORIGIN      
        1 caaaacctcc accgaggtca tcgcttacaa tcatgtcaac atcaaggcct tcaatgattc
       61 tgtcaatcag cactatgggt atctcggact tcacaagatc cttcaaatga tcggaattgc
      121 tacgcgaaac aggagcgaga atcaagccat ctacctgctt ctgaaaaaat cgatctatag
      181 atgtcttctc gacctccact ttttcgtcgg tacttgtgac aatcaaactg tacccttcgg
      241 gaacaatgac atcgcttatc gctctcgcta tcgtggagaa aaagggattc tgaatatcgc
      301 ccacgatcaa tccgatattc ttcgtctcac cggtagccat gctcttggca atgtagtttg
      361 gcttgtagtt aagtttcttt gcagccgcta tgaccttttc tctcgccttc ttgctgacgt
      421 atccatattt cccgatcgcc ctcgctaccg ttgccctcga gaggcccgtc tctttcgcaa
      481 tgtcgtccag cgttgttgcc ataactcatc acctgctcaa aatccagata catgattcat
      541 tatgataccg ctatcatata ttaattcatt ctatcccaaa atcagatatc atgtcaagtt
      601 tttttctcgt tttaagttag ttcatggatg ggtatttaat ttctctcagt tttttgttat
      661 ataattgact caagataact atgataccgc tatcatattt tttggtggct ttgttcgatt
      721 ctctcgttaa ttttgcttac ggaggtgcct ctttggaaag gattgacaaa ttctctaaaa
      781 ggttcgacag tacgcttctg aaagcgaccg ctacaataaa agagtcagaa gtctttatag
      841 acgatagcgt atcttgtgat tttagagcga ttgttgttcc gtggtatttg atggacagag
      901 tggttaagaa ggtcagaggt acggatattt cggcggcctg cggttcgggt tttcccttcg
      961 ggttcgagac aacggaaact aaggcttata tgatcagaga gtcgcttcgg ctcggccccg
     1021 aagtgagaga catagacata acagccaaca tctccgctat gaagtccgga gactgggatt
     1081 actttgaaag ggaggtctct tatctttccg gtctcctgaa ggatgtcacc tgcaaggtaa
     1141 ttatcgaagt atcttatctc gatacagtcg aaattgaaaa ggcttgctct attctgatga
     1201 agctacctca cgtcgatttc gtaaagaccg gtacgggata cggttcgaga cccactacat
     1261 tagaggacgt caggattatc aagagagtcg tgggagatga gaaaggaata aaggtatcag
     1321 ggggtgtgaa atctctctct caagttgagg atttcatcgc tgcgggagcg gatatcttcg
     1381 gttccagcaa tgcggtagca atatacaggg aatttaccga aaaataccct gggagaaccg
     1441 atcttgcatg aaaaaataat aatggaggca taaatgagac tgtttataga tgatggaaac
     1501 gtccaggcaa taagtgagtt gttcgattta ggtttctttt ccgggataag tactaatcca
     1561 agtattttga agagaacggg aagaaaaccg ctcgaagtca taaaggatat gttgaatagg
     1621 ttcactggct ctctttttgt ccagtgtgcc tctagaggtc acgatgaagt gctaaaggag
     1681 ggacaggaac tcttcgggct agaccccgaa aggataattc tcaagatccc cttctcgcga
     1741 acgggcataa gggtcttgag agaattcaag aagatgaata taaatacttt gattaccggt
     1801 gttttctccg ttcctcaagt gctgatgtct gcagaagcag gcgccgacta tgtggcgcca
     1861 tatgcgaaca ggatcgaaaa cctgggaata gatctctctg ttgtcggtag tatgcagaaa
     1921 gtcttgtcca acggcgacta cgagacgaag ttgattgccg caagcttcaa gaccctgaca
     1981 cagatctcca agatggccga gctacctatt tacggaatgt cgcttcctgt aggtatgtac
     2041 aacgagttct tctcacacag cggcacattg aaggcgatcg acaatttcga ggccgattgg
     2101 gattctattg aagaccgtga atgggtccct ctgaaatcat gagataccat tctatctgaa
     2161 agcgaggttt tgccttgaag tatataatgg cacatgatct gggaacaact ggagataaag
     2221 cgactttgtt cagccaaaca ggttctctcg ttgcttcatc attcgccggt tatgaaacct
     2281 tctaccccgg gcccggaatg gttgagcaag atcccgaggc atggtggact gccttctgca
     2341 agactacttc cgaaatcgtt cagatggcgg aaatcgacac aaaaaacatt tctgcactca
     2401 gtattagcgg ccagatgatg gcagtggtgc ctgtagatag atcgggcgaa ctgctcagaa
     2461 ggtcaataat ttgggcagat tcgagaagca ccgaacaggt agaagaactt ttagcgaaag
     2521 ttccggcaag cgatctgtac aaaataaccg gttccaggct ctcaccaacc tatcaggggt
     2581 tgaagatagc ctggctgaaa aagtacgaac ccgatctcta tgaaagaact ttcaagtttc
     2641 ttcaagcaaa ggattacatc aatcttcgcc tgacaggaag gtttgcgaca gatttttccg
     2701 atgccgtcat gacagctcta tttgatatct cgagtcttac gtggtctgaa gaactgattt
     2761 ccgctctcgg aattgaaggc tcaaagcttc cagatgtgta cgcttccatt acagatcttg
     2821 gagaaataag gaaagaggtg gcgacagaca tcggtcttcc aaactcatgc agagtaattc
     2881 tcggggcggg agacggttgt agtgcggccg tgggagcagg cgcagttgaa gtgggcgata
     2941 cttaccttta tctagggtcc tcggcctgga tttccacaat tacagaagag cctctacttg
     3001 ataaggaaat gcgagtgttc tctggggcac atgcagtaag aggcctctac tttccgtccg
     3061 gcactatgca ggccgccgga acctcataca gctggattaa ggatactatg tactgtgatg
     3121 gatccatgaa agaaagcgat cgtgatatct attcttatct cgacacccaa ctctgccaat
     3181 ctcaccgtca tagcgaatca atactatatc ttccgtatct gcttggagag agaagtccga
     3241 tgtggaattc caatgcaaga ggtactttca taggattgtc ggcctcccac aagaaggtcg
     3301 atatcgtgcg agcggtaatc gaaggcgttt ctatgaatct gaaatggatt ctggaaagtc
     3361 tggaagaatc cttgcctata ggtaagatca gggttattgg aggagtaata aaaagcagaa
     3421 tttggaagcg aattatctca aacattctcg gaaagactat caccattccg gaaaacgtcg
     3481 atgaagctac ttctatcgga gcggccatga ttgccggagt aggtgccggt ctttttaatt
     3541 tctccgcggt tcggaatttc gtcgttgatg tcgaagaaat cgagccgatc ggggaacagc
     3601 aagaagatta cctgagactc tacgagctgt tcaaagaatc atatcttgct ctagagaaga
     3661 catttgaagg gcttaacgga gtcagaaggg gttaggctaa cgttacggga gttgtactga
     3721 aacagggtgt cgcggatctt ttagcataat ggctgcgaca ccctgttctt tttttccttc
     3781 tggaaacctt cttcaaaaaa gccctcaatg tagggaaatt cttttcctta acaaacctct
     3841 cttttgaaca tactaagttc ataacccttg tttttcgaca ataatcacct tgaaatggct
     3901 tcctacgcat gttcaaaagt gttgtctcct aggtcaatta cagaatcatt ttgctccatt
     3961 tttcccttct gctaacctcg ttttgaacat cactgctcca ctaattctca acaattaatg
     4021 aagaccattt gtgtatccag ctataatggc taattacaac ttatacgctt ttgaacaacc
     4081 ccaaaactct cttctaaaca gccatctcaa ggttgtctac attcaatgtt caaagcgaaa
     4141 aagttttgaa catgactcca aacacttagc agaaaagaga tctgtgagtt ttcgacgagt
     4201 tttgaacata cctaattatt attatctata aagatatata aaataagaaa aagacctata
     4261 ctacactcca tcaaaaccaa ctatattgaa aattacttac cataatcgat tacacaaatc
     4321 tgaaaaatat agtagacttt caggcaaatc aaaaagcatc tatgactgaa aattctttat
     4381 ccacaattat cgactcatgc tcacttccat tagaaaaatc aaccttattt agatctacaa
     4441 gaaacagact ggagcaatca taagacctga aatacaactt gaggttctta ttgtccttta
     4501 tggtaaccca ctgagtgttc tcgaaagatg caggtccatc tccttctctc tttgaaaccc
     4561 cggaaggaat gtccactgta tttagaagat gaagggcaag cattactcct tcctcttccg
     4621 tttcaacggg atttgaaaac tctcttaaca gagctatcct aacaaatcta gagggaggag
     4681 ttccatcacc gggaattcca acatatccac tgccaaagcc catttcctta tatgttattc
     4741 cgtttatttc accttctttc atatatggac taagattaac gtagtttctc agattttcga
     4801 gatgcgatga aagctctgga gcattcgtaa gaactgcatt aggattatcg tagattttca
     4861 tctcaccacc aatgaactcg acaaccatgc ttccaccaaa cttgtcatgc aaagagagat
     4921 gaagaggggg aacttcaccg agcagttcat tttcttcacc ccaaattgcc atactttcaa
     4981 gagcttctct tacagattca agtgtgtcaa agtttccaag aatccaggaa ggaagatcga
     5041 acagctctat aacatttgta ggatctgatt cagtagagac ctgttcgtac tcagttcccg
     5101 gcaaccacaa tgcgctcaaa gacaatccca tttcattcaa tccatctgaa attgctgtgg
     5161 gaatatcaaa agcattcaat cccacaaatc catatttatt ttcccacgag gccgcttcag
     5221 acccatcggg catatgactg ctaaaactgt cacccctgtt gaagaataca actttcgagt
     5281 tcaaaggata tagtaactcc atacttctac ccgagataac tataccgttt tttttcaggc
     5341 gaaagaagaa actccgtaca tccaaccgaa gcggaaatga aagctaacaa aagaaagacc
     5401 actgtaaatt ttcttaccta attacatctt tctttttttg cggcaaatct aaaaatacaa
     5461 tgacgattga accatgaagt ccacgagagg tttccaaaaa atttaacaca gaaaagagga
     5521 gtgtgacaac aaagtaaaaa cctaatcagg aacaaagcat gactattata tctaatctac
     5581 acttataaga tttgcctttt taagcagtaa ttatgaagaa aaaaagaagg tgggatctag
     5641 attcatatat cgcgatccca tctcaatttt gaagatttat ttgaattccg cttaactgaa
     5701 aatcaacgct tctatgcaga ctctttgccc ataaacgcta gaacaatgtg gtttacacca
     5761 aaaacaagga gagttatccc aatcattacg gcaaccgaca gaccggcaat gagcgggtgt
     5821 agaacgagta ttattcctcc aacgagaaca agtacattta ggactatgct caaaaagtag
     5881 attccctttc ccgccggttt caatctatcg gcgtttatca ggttgttaat ggcatccaca
     5941 ataaaccata tcgctattat gaaagcaaat acatttgcgg caaaaagcgg tctgaagaga
     6001 aatactaccc ccgtaatagc caacaatatt cctagaatta gcgcgaagaa aaccttagct
     6061 ccggttcggt tttcaagttt gaagaaggca actatcaaca taattccacg aacaagaaca
     6121 acgataccta acattattgc aagggtaagg aacgttgcaa gaggattgct cagagaaaaa
     6181 atacctccaa ttatgagcac aaaacctacg agaagagaga tccagtcgaa cttctttgct
     6241 tctgccatgc tatccaccac cttcacccgt tagcatataa cgggtctatt gtaaatcaat
     6301 tgctcttcgt aagtatcaga cgccactgtg ttccatgaag cccaaccgaa ctccacttga
     6361 cttccttcac aacaaccgta tccattccgc gtgaaaagaa tctgtactct ccccacccgg
     6421 attccttttg agatatctca atacccaata ctcttagctc ctcaaagggc tcgtaaagct
     6481 ggtcagtaaa gaggttcctt cccatttcca tttcatcctg atcatagaat atcaacccat
     6541 ctggctgcat tacccaaaat tcataaggtt catagttctt cgggattatt aggggtgcaa
     6601 ggaaggtttc cggctttatc aagagactta gagaaccgga tagttcaccc tccttcgtaa
     6661 atactggcca ttcaagatca agagcaggga aaccctcaac agcaacaaaa ctctgactca
     6721 atacaggtaa tcccctcctg tggagttcaa ctacctgttc ttgatctcca atccagtctc
     6781 cttcaacata atcgaattca tcagggtata tatacttcaa aacaccttct atgtctatga
     6841 aagatacgtt tacaacctga ggattgtttg agtacagatc tttcagaatt ttccttatgc
     6901 cggaagggtc ctgaagaacg ctccttatct cttccgctcc ctttgcaagg gaactgtcca
     6961 tgagattgaa ctgctcctga atgcttttct gtacactatt tagaatgaga tccatgtcct
     7021 tctcttctgc aacatcactt atcggtttgg atatcacaaa tgtccaaccg cattgtgttg
     7081 acttcttgaa gtaagcagta ttcaagctct caccgtccga atagatcaaa ctcccatcat
     7141 ccgctgagag tcctcccaat ctttcgctta aaccgaccac cgggtagtca agagaaccga
     7201 ggagaagtgc ggtatcgtct gtcatagcaa gtgaattctg tacatcgaag acgtagaagt
     7261 gaatatctgg aagcagaact aaacgttcta tcaatgagaa attccagttt tcaaaaaagg
     7321 cagatagacc tacgaaagcc gaaatcttac cgttatccat aacaggaact gcgaagacta
     7381 ccgatctctt tcccgtagat cgactaagta cctgatagcc cagaacctct tttccttcca
     7441 aaagatccgg aaagtaaccg cgattcgaca gattcaaccc ggtccagtct ctctgaacgg
     7501 tatagtatcc accatcgggg aggatatata gagcaagccc ttcatagtta atgttgaatt
     7561 tttcaagcag gggtcggatt agtgaccagt ctccagactt aacttcagcg caggatgcta
     7621 aaatcctcag tgaactaata attcctaaca gccgctcttc aaaagacgtc atgataaggt
     7681 tcagaatact ctgagctggg atcgaccagt ctgtgataac ttcatcaact atgtcggtcc
     7741 agccaacatc tagctctata ttccacatac tgagagcttc ggtattcaga agtgttttcg
     7801 gattcttcga agtctctatc tcgatttctg atgcggaact tcccgagagg atcctggatg
     7861 ccagaatcgc tacctgtctt ccctcttcga aaccagaatt tacgactccc ccgacagctc
     7921 cgtctcgaac aatgaagtca gatacaccga agactggacc agaggaattt tccaccgtcc
     7981 actgtgtgat atcgtgagcg ccgattgtat tgccagatcc gtcttctata gtcctgactg
     8041 atagaagcac taccgcttcg cgaggggaaa ctccttctat gtagtttttc caatcctcga
     8101 aaactttgaa cccgtctatc tctaccgatg tatctccaaa atcgagcctt tgtatctgtt
     8161 ccttcattcc ttcaaatgtc tcgctgtcat ctccgataaa tcgaacggct tgtatctctg
     8221 gaaagagttc aaggatcata gagattgttc cattggcgaa gattgtctct attacaccag
     8281 tttgatttgc gttggctgaa taatggatcg aaggatcgag atttacaccg caaaagacaa
     8341 ccggcgcatc atgataatct tttgcgaagt attccatcgc attgtcatca caggcaatca
     8401 atatgtccgg attgaatgca gacgcctctt ctgaggctaa gactccagac ttgatcttcc
     8461 attcttccga agtctgaagg cgcgtatcca tgtaaaagac tttccactcg tacgaaggat
     8521 cggacaatac ggtttgaata ccatcaagaa gctctctaga ccagctataa tcgttatcgt
     8581 agctctggac aaccattatc ttcgtagcta aaatatgaga gcaaaagaga atcgaaataa
     8641 tcaaaatgac caatggtact tttctcacta agaatccccc cgtagaaatt attggagccc
     8701 aaacaagtgg attatatgat atacgagtgc caactcaagg caatctagag tgtgtggtac
     8761 ctcagcagat gaaaagattc agagtgcgaa atctgccgtg aagcaaagga aaatcggtca
     8821 ttataacgct tcggaatctg aattcttagt ttgcttcttc ctgaaaaact gtactacggc
     8881 aaagtcattt gtcgccattg caagcgtatc taaccttctc cctgtgtaca tccagggcag
     8941 aagaaagaag atcacctggg ccggagggat gttgaacgga agcacaacca tcgagatagt
     9001 gaagaggtcc agtgggctgg gaacgccaat cgatgcaaat acggcaatga tggatgaaaa
     9061 cattatgtac aggtaatctc cagcgttcaa agactttccg agaagctgca tgttgaagat
     9121 agctacgacc acaaaaaggg aaaccgtttg aggctgaaac ataaccagaa gcaaaggaat
     9181 gacgcctttc tgattttcat cgttgaactg ttcgcttttc tcacatgtct tcaccgcttc
     9241 gggaataacg gcaaccgaag aaagaagggc gaacaaaagc atcatcggct tctttattac
     9301 tccgaggtac tttttcgggg agagagaagc cttcctggct ccaagtaatg tgccgatgag
     9361 gaaccagata atgaaacata cgatttcaca tactatgagc attactagag ctatcagcac
     9421 ttcagtatct ctgatgaaga tcgaggcctg cccaagcatg agactgaaaa ttcctattgg
     9481 aagaacgtag taggaaagcc tgaagagaaa gaggaaggca tcatttattg tctctactga
     9541 atttacgatg ccggttctct tagaagattg aatatacctc actgcgaagg caaagattat
     9601 tgaaaagaag acaatagtca gagttctgtt ctctgaaaat gccttaacca cgctattacc
     9661 aatcatattt gaaggtaagg cccaaattat ggagcctgcc gaaagctcca cttcttcata
     9721 tggatctgtc tctctcatcg aagaaactag agcctcctga gcttcgatag ggattccaac
     9781 gccgggtttc aggactaatg ccgtaagaag tccgattgcc gccccaatga caaagaagaa
     9841 gatcattacc atagcgaatc gtttaatgaa acgcgcccca tctgatcttt caatgagctt
     9901 tagaaagttt agcgtaacga cgcagaacaa aatcggaaga ccgcagaaca gaagataatt
     9961 gaaaaagaga gttccgatgg gacttagaaa atcaattatc tcgggaaatg cgccacccac
    10021 atagaatccc aataccattg aaaagagaat tgtccatata ttcagtagcc agtgtggaat
    10081 atcctcaggt gatttatttc ttcgtagctt attttggcta ttgtgaacga ctacaatgga
    10141 aaaaacgatc agcagaacca gaattatcca tctgtatgga tcgccgacca agagaatctc
    10201 ttcttttttg tcaactggat cgtatagctt cgaaacgaga tcgaagtcaa tgtacttaga
    10261 gacaaactct tctgtacggt tgtattcgct gctcaaaaac ctgttgatcc actcgagaag
    10321 ctccggatta ttctggctta caccgattac aatctgatcc tcgttaggca acagatagag
    10381 agagtaatgg aggagaagag aggtgtttct gacgagaaag gactcagtcc agagattgtt
    10441 caggaagaaa gcttcttccg ttctctgaac taaccttctt gaagcttctt cggtactatc
    10501 tacggccgtt actcttgcat ttgggaagta ctgtttggca agggattcgt atacagtgtc
    10561 tgctacagtc attattgaaa ccactggtct gttaagagtc tcgaatgctt cttcaacgga
    10621 catttccgga tcgttcttct tgatctcttc gaaaagctga gtgttgacca gcacggaaag
    10681 tctctctgta agataggcac gtgacaggaa tatctgtgat gctctgcgag gcgaatatga
    10741 gtaataactg agaataatat cgccctcccc ggtactaagg ttttctgcaa gagtctcatg
    10801 acctgaagca gttcgaatga actcagcttc tacgcccaat tgagaagcga tatgtcttcc
    10861 aagttctatg tcgatacctg agagattgcc gtcgctgtca agataataga agggatacca
    10921 gtagtcttct gtctgcaaaa tccgtaatac gccggatttc ttaatctctt cgatgcttct
    10981 gaactctcgt gcgtagataa ttgcacaaag caaagcaaaa agaagtacca ccccgattct
    11041 cttcatggaa ttcccccgca aagcatttga gagattgtat cattctcacg gaaaaaggca
    11101 ttccaacatc aagacattat caaaggaatt gcaaaactaa ctagccaaga gctttctcca
    11161 ccctgtcagc agcgattttt gctgtaataa ggcatacagg aagaccggcc ggatcgaagc
    11221 tccactgacc tacttgaaag agattcttca ccggtgtatt gatcgatttc ttcatcttca
    11281 tgaagtccct caaaggaaat gtctcggaag gatgataatt ccatccagta atcgaaccac
    11341 cgctgttcaa ggtcttctct tccatcgtga aaggagtagc gacgatcctg aactcaactt
    11401 tctccttaag tgaagggtat agtctaaaga agttggccat tacatcatcc gccacctctt
    11461 ctttcgtacg ttcatattcc tccacagaaa tcccctttcc ccagccgttc attgcctttt
    11521 gagtcgtatt caagttcaga attattcctg tcttgccttc tggagcaaga gacgggtctc
    11581 taaggcaggg aatgctgatc tccattcccg ctgtcttgaa gtaatctgga gattcatagt
    11641 cgcagtattt tgcagcgacg ggaacataga ttatgtgttc tacaggttta tcgagatttt
    11701 ccggaggtgt atcaactccc ataaatacgg agaagacaga gtcggaaagc tgcgctctgt
    11761 tcagtctctg taagaagtct ttgtcgaggg tattctcagg aagcagcctg ttgtacaaac
    11821 gcttagcgtc tgcagcgtta acgatggagg atgccttcaa ctcgtcaccg gtatctgtaa
    11881 ccaccccgac cggcaagttg cgttcgagaa taatcttcgt aacttcggtg tttgtaagga
    11941 cggtccctcc ggtatttctc acgaactctg caatttcgag gggtatcctt tctatcccac
    12001 ctttggggta gaagtaatcc ctgaagacag ttgggtaggc gagaccgaag aatgcaggag
    12061 tcccgtcgaa aaagggttca tcgatgatac ttaccagctc gtcgtcgagt attctggctc
    12121 tcaagaagtc cctgaacggc acattgttgt atttgagttt cttcaagaag cctagaagct
    12181 tcttgccgtt cttgaaaaac cagggaattg atttcaactt agtcagaaac gtagtctttc
    12241 cataaagcgg tcccgacatt cccgataacg aacccatgaa gctgttcagt tcttccattt
    12301 ccttaagtag atcctctatg ccggatctgc tttcggggaa ccgttcgccg aggaagtcga
    12361 agtacgccag aaggctttcg tcagactcca tagaaaatac tttgtcctcg aaaccagcgt
    12421 acactctgtt ctttacgaaa tccagcttat ccatgatacc gaagttctct agcattggaa
    12481 atagaagccc ggcgttttcc accgccttga ttccgctgtc gaaatggtaa cctctgtact
    12541 gaaatgacga gaaacagccg ccgggttttt ccgctctctc aacaagtgca actttctttc
    12601 ccgccttgga caagaaggcg gcacaactga ggccagctat tcccgcaccg ataacaatac
    12661 agtcgaattt catacaatca cttccttttc aagccagctc aagagccttt taattggtac
    12721 gggcttttta tcgaaggaaa gtctcttaca gcttttctca cagaaaccgg cgcagtctga
    12781 gcaaagtgtg ccgattgtag tctcaagtaa ccgcttctta gcacctatga aattgcccgc
    12841 ctcgagacgc ctgagaattc ctcgaatatc ctttccatca gggcacttta atgtgcaatt
    12901 cggaatgtcg cagagctgac aggaactgct aatcaatgga acggtctctc tgtctagaac
    12961 tgtattctca agatctactg gaaccttttt ctttcccgca gtgtagtgaa ctctctcgac
    13021 ttcaggctcc ctgtagctgt attccttcat ctttagcttt ctaagaacaa gatttgcagc
    13081 tccgatcccg gagaccgtaa ctgcaggtgt ccccattccc ataactgttg aatctccgca
    13141 gataaagagg ttttcccatt cgcttttggc atgtagtctc tttagcaatt cctgacctat
    13201 ggattgcttc ggaccgccaa cgcgaccctc gttcttgagg gtatatcttt cgattgtaag
    13261 tggagttgcc gtctccagat gtttcaagga ctctctcagt ttggggaatc tcttttctat
    13321 cagtttaata acccgctcgg tctcgtcctc tttcatctca cggtattcct ttgtgccgcg
    13381 aggcggccac ctcttctttg agggagctat catggacatc acgtgagttc cttctggaca
    13441 tatagagggt tcgtcaatcg aaggtatgta tactgtgaca tcgctttcgg ctatttcgtc
    13501 aaattcctcg acaaacattt ctaccggatt cgttccggcc gggatagcag aagagtctac
    13561 ggcggcgtac acgaccatgg cgggaaaagt cggctcaagg gaccccgccc atttaacact
    13621 ttcctgacta acatactctt tgggaacgat acctccatag agattccaga ccgttccccc
    13681 gtaaatcacg aagtcagatc ttatttcgct cccatcttcg agccttatcc ccgttgcctt
    13741 cccatcatca aagagtattt cattaacgcg attttcgtaa agcatgtaac cgccgttctt
    13801 ctcgatcgcc ttttcaagtt tgttccgtat catctgagaa gaacctacgg gatagaagcc
    13861 acctccttcg tgattgtcaa taaacatagt tacggccagg acgccggggg tttcctttac
    13921 agtcgtatag cagtaggtgg aagttagaac atcgaaaaac tgatagagct ccttcgcttc
    13981 cgggtctata aaactgctca taagttcttc agtgcttttg aatagaagcc tgatcatttt
    14041 ggcctgtcgc acaggatgct tcagcagaga tctgagactg tcgcttctct ttatttcggt
    14101 gggaggtaca attacaggat ctgcaactat cacgttatga tataagtcgg ctagatagtt
    14161 gtagaacttt cttagctgtt cttcataacc tggaaagtta tttccaagct cttctataaa
    14221 tcggtcgaga tccggccaaa agacgattct cttctctcca aaaatcacag tgtatagtgc
    14281 ctcgtgtctt accatatcaa tgtcttcacc gatctcgttg aaaacaaacc tgtgagaatt
    14341 gaagccgcgg tttccgaatc cgaataccat gccgactgca gaatcaaacg tgaagtcatt
    14401 tcttctgaag atcgagcaac atcctccggg aagataatgc tgctccgcaa caagcacttt
    14461 cagtccctta ttagccagca gagaggcagc cgataaactt ccgagtccag atccaattac
    14521 cgcaacgtcg tatttgcttt cgggaaagaa cttaccgttc atatacaccc cccgatcaat
    14581 aaatgcatct tgtctataag aattctaaat tattggaatg cccatctcct agagttttat
    14641 gcagcttcgg aaaagtagat tggcagagga taaggaatag taaaggcgga gcaaaatgct
    14701 ccgcctgaaa gatctgatta tcgctcaata gtggtacgag gctaacttta aggaatcatc
    14761 actgtatcta ttacgtggat tacgccattt gccgcaatta tatccgttac tattatccct
    14821 gatccttgaa tcttgacttc ggttgggttt atcatcagct gaccgccatt aagagcggtc
    14881 aaagatctca tacccagtac atcttcagcc atgtattctc ccgatacgac atggtatagg
    14941 aggatgttct ttagagtcgg tatatccttg agcaacgatt cgatagtccc ttcgggtaat
    15001 tttgcaaagg cttcatcatt tggagcgaag actgtgaaag gcccatcacc cataagcgct
    15061 tcgactagac cggcttcctg aagtgctttt accagtgttg taaaggagcc cgcattgata
    15121 gcaatttcag ggattctcct tgtctctctt tccgggggaa aaatgaccgt gtcaataaca
    15181 tgaattactc cattgcttgc ctcaatatca actgccgtta tcattgcatt actgacgtaa
    15241 acctttcctt cttctacttt tattggtata gagctgcctt ctactgactt gagcgattca
    15301 agagaggcca cgtctgaaga catgtacttt ccaggaacca catgatatgt aagaattctt
    15361 gtaagtgccg gtatatcgtt taggagactt tcaacggttc cctccggaag ctttgcaaac
    15421 gcttcgtcag ttggagcgaa cacagtaaaa ggtccatctc ctattagagt ctccactaga
    15481 cccgcctttt caactgctgc tacgagtgta gtgaaattgc ctgctgaaac cgctacttct
    15541 actatgttct tggagaaacc agtgaaaact aatcctatta aaactaatac cagaaatacc
    15601 tttttcatca ttatcacctc aatcagaata ttttgtacta tatttttcaa aaccaaatcg
    15661 ttgacaattt gattttactc tgccaatttc atcgttgtca atagagttgt gcgcaatgaa
    15721 aaatcggtga ctgtgatttc tctttggctc tttgaaaaag caacatagaa ataatcatac
    15781 ggacgcttaa ctaattctta atctatgctt tacttcgatc attatggtta tcttgaaaga
    15841 ttagtagttg acaagagata agcgactttg gaagtgagat ttgagaagac attgattggt
    15901 tcctcaaatt ggcaatcaca tacgggtaga tctcaactcg taggagccct tttcggtaag
    15961 cttgaggtgt ccgtcgtact cggttatcaa catttcctta tctagcgcct cgagggcggt
    16021 cttaactatc gattcgtcag actgcgaacc ggaaattcct aaggctctga gtggttctat
    16081 atcttgataa gccatagatg cctttgcttc gcactcgagc tgatcgcgag tctgtatgtt
    16141 cccttgatct atcatgaaaa ggaaccagtg ctttagctgt ttcgctctga acttgttgaa
    16201 gagagaaaag ccatatcttt cgaagtcgat ggcctctcct ctggcagatc ttataagatc
    16261 tatcagtaac tctgccgccc ctttgagaat cagaatggaa acgatcagcc ctacaatagc
    16321 gtcggcgtag ggaatcctca tgagagatac gataaggcca attgctacgc taacggctac
    16381 tagaacatga tttcttgaat ccgccgactg ggtaatcagc gggaaggatc ggttcttgag
    16441 gcctgagtac ttctgataga accacaagag agcgcagact attcccgaaa ttgccactgc
    16501 agtaaaagta aggagatcag gagaagggat ctcaccggaa atgattctga acagagcctc
    16561 tatcaatgaa aaaacaccag ttactcccat aaataataga agaattaggc tgaccagatg
    16621 ttcatggttt ttcttcactc cccagtacac gaagacactt gagattccgt ccagaagagt
    16681 atcgaacgaa tcatttagaa ggcctacact gccggaaaga atagcggtgg gaagcttcaa
    16741 ggcagcaagg atgaagtgaa ctactatcgt tactctcgag ccagtttcgg ctcttgtcgc
    16801 tttttccaga agtctgccac tattctcgag ttcggcgagc atcttccttg cctcaaactc
    16861 tcccctctca gtcagttcaa atgtgtcgct attctgaatt aaccactcat cttttttcat
    16921 gtcttctata gcggaatcca gaaatgaatc tagatcagtg gcttcgtgca tcctcttgcc
    16981 atagggaaag acctcgaaga aaatgccgaa ccttctcccc atcactgaga agtactcttt
    17041 cagtaattcc ttcgtattgc agcctctgag aaccgcggcg gccacattcc attcatgtcc
    17101 ataatgcttc gaatgaactc ttcgtaacaa acggcccacc ctctgacaaa aaatgattcc
    17161 ctgctactca tttttgttta aataaattcg atcgacccct ttgagtatat tacattgaga
    17221 agccttatcg tacttcctca cttaggatgc tgttcaaaga gatttcgaca ttaatagatc
    17281 ctttatgcag agtaccgaaa cgaaagaaac ctgacgtatt ttgattccta taactgcgct
    17341 tacctccatt tagcagaaca aacatgctta atttgatctc tttttcggtg atattatctc
    17401 ccttggtgat gatcggaggt cattctcagt gcccaatatt tcaggagcta tttataagac
    17461 tatattgaac aagcggttta tttattaaga gacatctcgt aaaggagatg atgttctaga
    17521 tatctctgct attggcttta atcacatcga taattattcc ccctcgtatt gaaagtattg
    17581 actggcgagt aattgccgag ctcttaagcc tcatgatagt tgttcaggct cttaaggaat
    17641 acaaggttct agagaagata gctactatct cgctctcaaa tctaagaagc gctagaagca
    17701 tcggatcagt aattgtaata gggtgtctcg aaaagaaggg cctccaaaag attcaatggt
    17761 gataccattg aattgactaa gaactagaaa ggaggcccga atagatgtta tcacaaggga
    17821 tggagaaaaa ggaaatcatt aaggtactgg ataacctgga caaattcata gtaaagggaa
    17881 ctgtaaagaa agggagagga aggaacaaag agtacgacga gataacgata ttgaagttgc
    17941 tggttttgct gacacttgcg aagatatcgt atcgaggagc gaaggggttt cttgaaggga
    18001 atggtgagta tatcgagtat cttgggttga agacgatacc atcattccag accttgtctc
    18061 gaagagccag acagctacct tttcatgaaa tagtccagaa ggcaactaca ctcttcacag
    18121 atgcggaagg gctcgaaagg aaagaagttg cggtagactc gtttcgtgag caagtcatgt
    18181 aaaggttcta cggcgcagag aaggaggaag agagggaagt ataaagacca gggagccagc
    18241 tggacgaaga cgaacaaagg ctggcagtat ggaagaaagg tgcacatatc tcttgatctg
    18301 gtcaacaagc taataatcga ctggatcatt accggaggat cgacacacga ttcgaaggta
    18361 gcaccgaaga tggcagacac ctgtaaagga ttcaagtaca ttcttgcaga cagcgcatat
    18421 gattccaacc agttctacgg ttatatattc gagaagaccg gtttaatacc ggttattgac
    18481 ataaacagaa ggagaggtct agatacagaa aagcagaaac aattccgttg gctggcaatt
    18541 ggactgaaaa agcttcactc attgaggtac cagctaaggg aagagataga gagaaccaat
    18601 tcaatacttg aaggtatcct tgattccgaa ttcatatggt acacaagaaa cagagacttt
    18661 gacacagcca tcggccttaa gattctcact tacaatctcg tggtcattta caatcatcta
    18721 caccatcgct ctttgagaaa gattatggat atcatccatg ctatttgatt tgtgagacac
    18781 cctaaataat tgtaattcta acggctttca tgggcgcttt gattacaaat gatgttgccc
    18841 ttatcgcact tgttccactc acaatgacaa tagctgaaaa ggcaaaattc gagccggtgt
    18901 ggattgttat tattcagagt caggccgcta atattggcag cgcactgacg ccgataggaa
    18961 atccgcagaa tctttttctc ttcgaaaagt acgaaattgg tatctgggaa tttggcagcc
    19021 tcatgccgcc atttgttgtc tttggtgttt gctggactct cgtgatgaat cttctgaaca
    19081 ccaaaaggaa gattgttttc gatgttccat cttcggagat ccgtgaacct caaaagattg
    19141 attacttctt gttgggtacc tttttgcttt tctttgtctt tattgacaac atctcaagaa
    19201 tggagataat agctactatg atgaagtctg caacaaacgg tgagattagg acaatgacca
    19261 gctccgctct tctatcgcag tttatcagca acgtgcctgc agcgattctc ttttcatcat
    19321 ttaccgggag ctacaaagaa cttcttctgg gcgtaaatat tggaggcagt ggcaaaatca
    19381 ttgcctctct ggctaatctg atagcttata gaatctacgt caaagagaag ggtcgaaccc
    19441 tcaaatacat cgcagtcttc acggcatcta gttcgataac acttatctta agcatcgttt
    19501 tcggctactt aaggttgaaa cttacagggc tttagtagcc aacaattgag agctcagtta
    19561 tattataatc aagactgttt gggctcatct gcttagtgta caatagacga cccgttttcc
    19621 tatcataaac gtgttgattg gcgtacgtcc cgtttgtctg ctgaagcata attgcttcag
    19681 ttccatcttg aagctcaccg atataactca cacttgttac tacatttgtt atgggatctt
    19741 gatcaatgct ctgcaacttg tttagcgaat caagagcttt ctccggaagc caaatcggtc
    19801 cattcagctg agctacgccg gttacgttgt aattgtcccg cggaatgctc ccatactgcg
    19861 agatagtcat tttgaagtaa tcccaggaaa gcgatgatgt tgttggagtt attttgacct
    19921 gcatctgcgt gggagtcgta tatgatccag gtatatttat tgtatatgta cctgagtatg
    19981 atgtggttgt tagtttccaa gcgggtctct gtgagttgaa ccacggaagt tcgacatatc
    20041 tcgccgaaag aaactccaat atgttttgat ggatatcagt tcccgaagga gcttgagtca
    20101 agagataaag aagaattccc gatgctcgat cgtacgtgta tacatgctta gtgtttactc
    20161 ctctagattc aattcttact gcctgcacgg tctgaccacc gtactgaatt ggcatctcca
    20221 cagcggctgt attgtcgctg ttgaatctgg aaaggtcgtc gaaaactttg ggatttagcc
    20281 acatatctga cactcccggt ttctcgattg ttccaaacga aacgctggga tagtaaccac
    20341 ccttcatctc catgaaatac gtctgtaaga gagctagtga tccctgctcc actgccacca
    20401 tatccatttg atagagaccc acacctgaac caccggtatc cgataaacct cccccgggtt
    20461 tcatcacgga agtgcttgca cggaaactaa gtcgcaatcc tggagccatc cattcaagcg
    20521 gactgtccga gtattcaggg aagatagcta gccagacgct gctggcaata acgaaagaaa
    20581 gtgcaaaaaa cgcaacggac aacatgctta tgattcgttt agtcattcga cccacctcct
    20641 gttaaagaga ttgtactgca aagtgattcg ataagatgat gcttgaaaga ggggatttga
    20701 ggtagagtaa ggtaatacaa aagacaagat tcctccatta gtcaaagagc ttatcagtaa
    20761 gcttaaaatg tagattcact agaataccga ataaggagat tcattattct gaatggggaa
    20821 aacaccggaa ttgcccgtgg attaggttgt gggaacgctc ttgggataag atagaccaca
    20881 taagccaaat aaccaaaata gcgactttcg aaagtcaata accatttaga aaagcgcttg
    20941 agctttgctt ttcgggaggt ccataggtaa gaagtattga tcccaaaaca aattgcccta
    21001 gaacagttta gtatgcagac tcattttttt ctcctcagag cccttcatct tcttgctcaa
    21061 taattgacaa atcactcaaa caatgactct attttacaag gttcttgaga ggaaaagttc
    21121 taaaggagat tcactcctgg tatagagcct ttcttgaaaa tcgcgaccat gctgacagct
    21181 ccaatcagaa ctgatcccag cacaatttcc ttcaaataga aaggcagatt tatcatgaca
    21241 aatccatttg taagaaccga gaggagtagc gagcctatga cagttcccca gacgttcggg
    21301 accccgtccc gtagaaatac cgctccgata aacattgcac tgattcccgg cataagatat
    21361 ccgtcgccca ttccaggagc accggaaccg agaagcgaac ctatcattat tccggcaagc
    21421 cctgccatga atcccgaaat cagtaacgcg atgaacttta ccctgcgaac ctttattccc
    21481 acatgctccg atgcttttgg atttccgcca acagcgtgaa aaaatcttcc aatcttagta
    21541 tgctcgagaa gaactacaaa gacagtgatt actatgccga aaatcacgac aggcatgggg
    21601 atgatttcga acaggaaaga tctccccaag aaaggaaaga gatcggggaa tttggcggca
    21661 tagtaagtcg acccccctgt aagccacttt gtgaatcccg cgagcagagt catcattcca
    21721 atggtagtta ttatcgatgg aaccccaatg aagagtatga taagaccatt cagtgcaccg
    21781 attcccaggg ctgccagtac agataatatc catgctggaa ttgctcccat gaagactttc
    21841 cccatcaata tcaaacttag tacaccggag aagcctgcca atgatgcgaa tgacatatcg
    21901 aattcaccgg ctattacaac agtagttagc ccgatagcga ggattcccgt tatgcttgcc
    21961 tgtctaataa tcgatagaat atttcttaca ctataaaagg taggttccat gaatgagaat
    22021 acggcaaata cggcgatcaa tgcaaataac acaccgtatc ttctcactac tgaagccata
    22081 ttttcttttg agatcatgac caatcctcct catctaacat ttccaggtat tttccctcga
    22141 atttgaacac aaaatttgga atctaaatag ccttcgataa tatcttctca tcgtcgaatt
    22201 cttcacgatg tagctcaccg gctattttgc cttctttaaa aatgtaaagt ctatcgcaaa
    22261 ctcctataag ttcggggagc tcagaagaga taaagatcac accgacacct ttctctcctg
    22321 cgagatttct gatcatcttg tatatttcga acttcgttcc gacatctatt ccatttgtcg
    22381 gatcgtctag aatcaacacg ctcatgtgat cctttgagat ccatcggcct attacaactt
    22441 tttgctgatt accaccgctc agaacctgga cgtcctgatt gatgtttgaa tatttgagac
    22501 ttagtgagct cttgtcaagg atcgttttaa ccagagaatt tactttcctg gctgagacaa
    22561 gtcccaggag ttttgagaac tcgttaatat atgatattgt caaattcttc ttcaaattca
    22621 aacttgcaag taaacccttt tctcttctat catccggtac gagaaagaca ccttcatcaa
    22681 tcattttgtg gacgttggga tgaacttctt tccccttgac aattaccctg ccggtctttg
    22741 tttctcttaa accgtacaag tgctctatga actcagttct tccagatcca ataagtccgt
    22801 aaaatcctag aacttctccc tttcttaggt aaatgcttat gtccctaaga aatcctgaac
    22861 cgctgaggtt ttcaacctgc agtatagtat ccccggcacg attcgtcact tcgggaaaga
    22921 ggctggaaat attcctgtta accataagtc ttatcgcctc ttcttcagag agatcttctt
    22981 tatcccttgt gataatccgc tcaccatttc tgaatattga tatcctatcg gcgttttcga
    23041 agacttcctc aagtttgtgg gttataagga ttacagaaac tccttcatcc tttatctgat
    23101 gaatcgcttt gaacaatgat tggacttctg actcggttag acttgaagtg ggctcgtcca
    23161 agataagtat cctcggctta taaaggaagg cgcgcagaat ctctatagcc tgcctttggg
    23221 cagcgctcaa aagcgacacg ggtacgtcaa gaggcacttt aaggtcgtac aaagcagcta
    23281 tcttttctgc tttttttaga atggatctct cgtctattaa gaaccctccc ctaaccggtt
    23341 cttctctaag aagaatgttt tgagcaccgg tgagatatgg gattaagttc cgctcctgat
    23401 aaaccattct tatacccatt ttaagtgcct gggaaggatg cgtgagtcta atttcttctc
    23461 catttagaaa ggtctttcca tcatcaggtc tgactattcc ggtcaaaaca ttgcagaaag
    23521 tgctctttcc tgcaccgttt tcaccacaaa tagcgtgaat ctcgccctcg aacaaatcaa
    23581 agtctacatt gttcagggct ttaacccctg gaaaggactt agatattgat acagtcttca
    23641 aaacaatact cttatccgac atgtcagacc ttctttcact aggtaattta gaatcaatat
    23701 cgggagagag aagcctctcc cgatattgtc tttcaaagcc ggcgattact gaatcttgta
    23761 tggaccgcca gcatcttgcc agttgaacca agcatctgga tcgtcaggat ccattccgta
    23821 ataaccaaaa acagcatgaa tagaagttcc tggttcggga acgtttgtct catctgttaa
    23881 atatggagtt gcatagatga ctcttccagg aggtacgaga gagtcaggat gatcaggtcc
    23941 tattcccgtt tctacctgat taatgacttc aaatacggtg tgaacgtagt actcaaatgg
    24001 ctgacttact gttgccacaa aaggagaacc gctgcggatc atagagtaca cagacgaacc
    24061 tccgtctata ccggtaacga agatatcatc cttgtcaaat cccgcttgtt caattgcacg
    24121 tgcggcgtac tgtccgggcg tgtcccatcc tgcaaatatc cagtcaagat catttccgta
    24181 tttcgtcacc cagtttacag caacgttgaa tgcgctttct tcgtatccaa ctagaggcag
    24241 aaactcttcc gcaacaagct caactgcagg ccagtctctc tcaaacaggg ttgcagctac
    24301 gtaagctctt cttcgcataa tgtgccattt tccgctcatt atggcgacct ttcctctttc
    24361 attcagtctt tggattcccc aataggtcat taaagctccc gcaacggcgt tgttctgtgt
    24421 gcagctcact atgacaccag gcttcagttc tgtatccaca cagtatacgc ctattccttg
    24481 ttcggcagcc ttgaggatca agtctgcaat agcctccatg tcccagtaga tgataactat
    24541 tgcatctaca tttttctgga tgagagtttc cattgtttct ctttgctgtg ctgcatctct
    24601 aacagtcata gcatcaatga actcccagtc cctgtgagcg cattcgattt tcacctgctg
    24661 gaaatctctc tggcaggatt cggcttcgag cccatgtcca caaattgcaa cagtaacgtc
    24721 ttttgcatat tcagttcctg cgaacgtcat gcctgaaagc gttagaacca tgattacaag
    24781 aattgaaata ataaaacgct tcttcattcc ttcacccctt ttcaagattt gtgtagttct
    24841 tctgaaactt ccctcagaaa gcggtaaaca actgtagctc cgggatcctg ctcgccgacc
    24901 gtgcggctgc cgaatgcttt tgctctaccc gtctttgctt ccatgttctt tgttctttcc
    24961 agtccatctt ttgcggcctc tgcagctttc tcaaaggcct ccttaataga cttgccttca
    25021 gaagcggcct tttcaagagc ttccacagca ggctcaagag catcgagcat cgttttttct
    25081 cccgtttttg cctttcctct cttcataatt ccctctagtg cagccctgaa cataccggcg
    25141 atttcatcca gagtgagaga ctcctttccc agaaccttct tgcctccctc aatgaatgcg
    25201 cttgccgtca gaactccgaa ggtggaggga tttgcttgat tgaactgaat tccacacttc
    25261 tttatcagag ccccagtatc ctcagtctga aaagtcttta gtgtttcgat tatcgccgta
    25321 cagccctttc ttatagtgat tcctagatct ccgtcaccta tagccgcgtc aagggctcta
    25381 aactcatcaa tatagttctt ttcaagtgaa ttgcaggcac catagagaat ctctgagatc
    25441 tttgctgcac taatggcttc tttcatttga ccaccgtccc tcagaattgc tgcagaagca
    25501 tcggcgaatg aaccggcata taggagctcc ttgaattcct tgtcaagttt gaagagagtt
    25561 atcgaaagac ctttcatctc cattgaagtt gcgtattctc ccacgaatga cttgtatata
    25621 ttgattttct tcagatcgag tatctccttg acctttctga aggttacata gagttcctga
    25681 ggtgctgtcg cacctagccc gttgataagc acggcaacct cgtctcccga ttcgaatgga
    25741 agatcgtcga ttactctctc cgtcatgaaa ctggcaatct cgtgggcact cttgagtttt
    25801 tcgcgcttta tacctcgctc accatgaatg cccatgccga tttccatttc atcatcacca
    25861 atctcaaaag taggcaatcc gactgcagga atcgtgcatg gtgaaagggc aactcccatt
    25921 gttctgataa cactcagagc tctgtcggtt gtttctttca cttcttcaag agaggcacca
    25981 gtggcagcct ttgcaccggc aatcttatat gcgaaaatca gccctgcgat tcctcttctt
    26041 gaaccggtat catctgctgg agccgatgct acatcgtcac aaccgatact tgtttcaacc
    26101 ctgattccct cttcctctgc catttcggcg gccatgtcga agttcattat gtctccgccg
    26161 taatttccgt acaggtacag tacgcctttc ccgctgtcta tggccttcgt aactgcaagc
    26221 atggtttcgg ccgaaggcga tgagaagacg tcgccaacgg cggcaccatc gagaaggttt
    26281 tcgcctatat atcccaggaa gaggggcagg tgaccggaac caccaccggt tgcgatgcct
    26341 accttgttct tcacgggtgc tcttggtgtg atcaaggctt tgtcaaagcc ttcgactctc
    26401 ttgaaatcct ttgggaaggc catgacaatt ccatcgagca tttccgtgat caaattgtca
    26461 gtggagttaa tgatcttttt catggttatt tgcctccaag ccacttcacc acatttaccc
    26521 agaattgacc gtaatctttc cattcgacca tcccgcggcc ccagtgagga gctggatcgg
    26581 ttgcgaatgc aagcgttcta ccctttccat actgaccggt cacgattatt ggatctccgt
    26641 tgtgttctgc caggacttct ccttcgggct ttgcctttac ttcgttgtat ccggagaaga
    26701 gagggggagc tgaatcgaag tctactccct tgaagatcgg atggtctttc ttgaggactt
    26761 taggagtcat gccttcggaa atttcgactc tgtcgtcgta tggataacag gtgacgggaa
    26821 gtatctcctc aacgggagtc ttatgccact tggctacccc aaacatgcct ccaaatgata
    26881 gccagccgcc tacaccgcag aagccgccgc catcacgcgt ccatttcttc agtgcgccga
    26941 gcctgtcgac tcccatgggg caattgttga acctgtcctc gtacattatc actgtgtctg
    27001 ttccaacatc actgaaaagt attacatcgt actggccaag ctcatccggt tttgggaagt
    27061 ttcgctggac ttcgcttgtc ggaagatacg tcaagtcgat ttccgaatcc gactcaagag
    27121 cctttctcag atgaaaactt tcatcatgta caactccatc tgtccaggaa ttcatacctt
    27181 ttacgtacgt tgtgatcagc atgacctgat ctccacaata gaatacttta agcactaaaa
    27241 tacctccttc tgattttgaa ttaggtcacc aatgggcctt tatttgaaac attcttcatt
    27301 aatgcaaaat tgtgatcaaa ttctcaaaga aaaaagcata aaggtgcccc ataactcaag
    27361 gcacccataa cctctaagct ctatgctttt ccatcgcacc aaccatgagc gcgaaggatt
    27421 cttattaaat tctatttcta gtattcccgg cggttctagc gcagctaatt agaacagatt
    27481 ttggtttcgg aaacagtgta atggttttct tgtcaaagtt tccttttact catagtaggt
    27541 aatcaggtaa actgtcttcg ttatcggttt ccacagatct tcatcttcga agtaaacaag
    27601 gtatctttcc tgaatacagt tccctctttc gtcgtagagg tattcatagc tgtaagtgag
    27661 cggacttccg ccgatatttc tgttatagtt agtctccttc agcttcagtc cttcactatt
    27721 gtattcggcc gagtatacag gattgctaga ttcagaaaga cagtcgacct cgagaaccat
    27781 attgccattc tcgtattgag cttcatgctt agagagtatc tttccatttg cgtcggatag
    27841 gatgaacctt atcaggttcc cttcatcatc gtaagaagct tcgtagaaag aaatgagcgt
    27901 atcgctcgca ttgtaattgt atgcctttat caaattacct tcttcgtcgt attcataacc
    27961 gtatttgtaa gagaagtctc cgtatttgcc gtaaccgatc tccagaagta agtttccaag
    28021 gtcatcatac tgatactcgt ttctgaattc agtttgaagt ccttcctctt ctttgtactc
    28081 ataaacaata tacttggttt cctcggtcat gtaaccgagt tcgttatagc tggctaagta
    28141 tcttgtgctg gttgttgaat catactgaac atcatttcct tcgacgaagt ttcctccttc
    28201 gtcgtacttt gcagagtatt tcgaggttat agaaccatct gtggagtact gaattccttc
    28261 gatctgtctc ccgttctcgt cgtatttgta ttcataactg tgttctaata cagagtcctc
    28321 aactatcctc ttcccctgca tcgtgatcat gttaccttct atatcgtact gataggaata
    28381 ttcaaactcc agaacaccat ctccggctac tttgctctca cggatagtat ttcctgattc
    28441 atcgtactca gcggtcagag tggcaaattt tatccacaac tccccgtcct gagtattata
    28501 gttttttatt tcaatccttt ttactccggt tgtgctaata ttatccagat aagaatcgac
    28561 ccagaaggcc ccacaaaagc ttccgatgaa catgaagata agtaataaga aacgacagag
    28621 tcttaaaatg cttgaaaaca aactaaccac cttccatgga caaagtctca ctacgttttt
    28681 ttgaagaagc gattttgaat gtctgagaaa atgactgcaa ccgctctagc acccgcgtcg
    28741 tatgcgccaa tgcttctgtc acccagatat ctggcccttc ctttcgtcgc aacaatcgtt
    28801 tttgtagatt cggctccttc ttctgctctg gttacggcct ttgaaaaggc ctcttctatt
    28861 ggttcgttca gctttgcact ttccttcaaa gattctttga aggggataag cgcatctaaa
    28921 agagttttgt cacctaattc agctttaccg accttttgca ctccatctac cattgcctgg
    28981 aagagttcgg cgatgtctgc aatttcgaga ctctcctttc ctttcgaagc tctagaagcg
    29041 ctcaaaaaag cattgcccca gattggaccg gaggctccac cgcatttttc cattattatc
    29101 atgctgcagt ctctcaaaaa ggttccaaca ttgctctttt cgagatcctg ccattttgac
    29161 ttgacttcag agaagccttt cgccagagaa gctccgaaat caccgtctcc ggcaacagaa
    29221 tcgagatcac agaagtactg ttcgttttcc agtatagagt cgacagcgaa tccaacgatt
    29281 tctatgaatt catccttcga gagtacagtc atgttctaag cctccgtttc cttccaggca
    29341 gcggtttcac agggtgcaga gagaagtctc ttcatctgtt cgtcgagctt aagaacggaa
    29401 atcgacccac cttccatatc tatagacgtc atgtaatttc caacaagagt ctttgcaacc
    29461 gttattccct tattagtaag cagcgaggct acctcgttgt ttaatatgaa aagctcttgc
    29521 aatggagttc caccgaatcc atttatcaac acggcaacct cttcttcctt tgacacggat
    29581 aattcaccaa gtattctttc gataatcagt tcagcggttt ctttcgccga gagtaatctt
    29641 tctctaaata ttccggcttc tccgtgaatt ccgacaccga cctcaatctt gtcctcttct
    29701 aggtcgaaag tcggttttcc tcttgcaggg acggtacagg aagtcagtgc aatccccatt
    29761 gttctaacat tgtcgataac tctttgggcg atctccttta cctgcctgag agaagctccc
    29821 ctttcggcca gagccccagc aattttgtga acgaagatcg ttccggccac gccccgtctg
    29881 tcttcctttt tttcgattga gatatcatca ttcacacaaa cgctatcaac tctaatatcg
    29941 taatcttcag ccattgcagc agcgctttcg aaattcatga cgtctccaga ataattcttt
    30001 ataaccagca aaaccccctg ctccgaatgg ttgtgtttta tggcgttgaa gacctgcatt
    30061 acagacggtg aagcaaagac atcaccacat acggccgcat caagcattcc cttgcctacg
    30121 aaccctgcat gagcaggttc atgaccactt ccaccgccac taattaggtt aaccttgttc
    30181 ttgtcgggat tctttcttga aatcaccatg aattcctcgt cgagtgcaag attctccggg
    30241 aaagccttca ccattccctg acacatttcg attaccacac ttttgggatc gttgatcagc
    30301 ttcttcatct ctctacacca ccttagctat tcttatacca tatctcttac tagttcttgc
    30361 tcggttggtt tccgcctctt atcggcatct acgataagcg agatatttcc atctttcata
    30421 atgactatcc tgttgctcaa tcctattagt tcgagcaagt cgtctgttat taagatgatg
    30481 gcaaggttct tatttgccgc ttctcttatg aaggtgtata tctcctcttt ggctccgaca
    30541 tctatgcctc tggtgggatt gtcgagaatc agaatatcga gattcttcat aatccatttg
    30601 gtcaatacga ttttttgttg gtttcctcca ctcaacgaaa agcacagatc ttcgctcgtt
    30661 gccttaattc ggaacttctc tattccctct ttgatcagtt tggattcttg cctgttgttt
    30721 ataaagccca gtcgactgct gaactcactg aatcttgtaa tcgtaagatt gttggcaacc
    30781 gttgacaagc cgattattcc gttttccttc ctttcggaag gaacataccc gactcttagc
    30841 ttggtcatat ttcttatcgt ggttttaggc acggaatgac catctataat gatttctcct
    30901 tctgttggct ctcccacccc aaaaagtgtc tcgcccagaa acttttttcc agagccaatc
    30961 agtccaccta ttccaaggat ctccccacga cggacttcca gagaaacaga gtcataaagg
    31021 cccttcttgg aaagatttct caccctcaag acgggatcct catccccatt gaattccttt
    31081 tgatcttcaa ctctgtaggt gtcggcactt aactctctcc ctaccataag cggatggatc
    31141 tctctctctt ccgaaacctc gggatcaact tctccaacca gctgcccatc tttgagaatt
    31201 agaatcccgt tacaggcatt catcaattct gaaagacggt gtgagacaaa tatgaatgtt
    31261 gccttgtttg aaaaactctt aattctgtcg agaagaatct ctctttcctt ttctgggagt
    31321 gcagcagttg gttcatcaag aagtattaga ggcgtcttga tcttgtacat gtctgctatt
    31381 acaaatgccc tgatgatctc aagcatctga cgttcatgga atgaatatct agagacttct
    31441 tttctggggt cgatatcgag cccaaaactc tccagatagc gcttcgcttc ggagatcatc
    31501 tgcctcttct tcagagtacc ggccacctgg aatctcttct cgtgtgaaag aaagagattt
    31561 tcgtaaacag tcaggctttg gacaaggttc tgctcctgga agaccatcga aactccgagt
    31621 gtattcgctt cccgaaagct ttgaggagca tacttctttc cttcgagaaa catctctcct
    31681 ttatcgggtg attcaactcc tgctatcagt ttaagcagag ttgactttcc cgctccattt
    31741 tccccggcaa acccaataac atcgttcctg ttgatattga aagtaacgtc ctttagtgcc
    31801 tgcacacctg gatagcttct ttcaattccc ttgatctcga agaggatttt gttgtttcct
    31861 tttatgtttt catccataca gataaacctc gcattctttg tctcacctat tggaatcaaa
    31921 gattacttaa cggtgattat ctttggtctt ccgacattgg acatgagcgc accggcaatt
    31981 attagagcgc ccatggcgac atttctgaac tcgggtgtaa cgcccatgaa attcaagccg
    32041 cttatcagaa cggtaaccag catcgctcca aggactgtgc cgattggtcc tccccggcct
    32101 ccgctaagag gaatgccacc catgacaact atcgcaatta ttggcaaggt ataggtatcc
    32161 ccgcagtatg ggcaagcggc ttcaagcata gatgcgtcaa gtgccccggc tagccctacg
    32221 agcgaaccat gaatcatgaa tacaaaagtc tgaatcttta caacactgat ccctgaatac
    32281 tggcatacat actcgttgtt gccaatagcg taaatcattc tgccaagcac tgtctttttg
    32341 gttattatta tggttataag cactacaatg attggtatag tgatcaagtt cgatatgcca
    32401 cctagaaagc tgcctcttgc gactgccaaa aaggattctg aatggacgtt gtaaggagaa
    32461 cctttaagta ttaagactgc agttccattt aagattccca tcatagcgat agtagataga
    32521 aacgtcggaa ttttgaggaa agtagccaga aggccgttta ttaaacctac tagagcccca
    32581 actatcattc ccactatgat cgatgcgccc ccaagtgccg agctgaccat tgcagaagcc
    32641 acggcggtaa tcgttatcgt cgctccgaca gagacatcta tgcttcccat cagaacgacc
    32701 atggacatgc ccattgatgc aatcagcaga atagccatct gtacgagcaa gttgttgaag
    32761 ttctttacgg acaagaacgt aggatactgc aaaccgaata tcaagaacaa aaccacaagc
    32821 agtaagggag tccacagctt tgaaagtggg aactttcgct tgccgtttcg attatccatc
    32881 atcattcacc tccctgccta cttagtaaga agtaatcttc ctcttctgtg aggaagcatt
    32941 acgagtgaaa ttgccgcgat taacagtata ccttttgcaa tgttctgtac attcgatgta
    33001 cttccaatta gactcattcc attatcgaac atactgatga gaagaacccc tatgagtgtc
    33061 ttcattaaac caccggaacc tcccatgata gaagttccac ctacaacgac ggctatcaga
    33121 gtattgaaga gatatttgtc tccaagtctg gacggggcca ttcccagata agaagagagc
    33181 agaaccgagg caatgccaac caacaagcct gaaaacataa acgcgattat cctgtattta
    33241 ttggtgttga ttcccatttt cttcacggat tcttcgttgc ttcctattgc caaaatgtat
    33301 cttcccacta ctgtcttcct gcttatgaat acgaaaactg caaatactat cagagaccaa
    33361 ataatgacgt ttggaatgcc ttcaatcatc tgtcccgtcg aaatccaggc aaagtccctg
    33421 tttctaatgg atatgttata accatttgtt agaccagtag ctattcccac atagacatac
    33481 atcatcgcca gtgtaacgat gaaagacgga agtttcagaa gtactaccag tagaccgtta
    33541 accagtcccg aaaggaatcc tacgactatc gcaagaacta tcatccctaa tccaccctga
    33601 ggaaagaagt aagcggcaac aatcgctgac gatgaaagca tggcgcccac agagagatct
    33661 atgctcccca tgagtatggg aaaagtctct ccggcggcca ccagaaggaa gacgctcgag
    33721 actgtcagaa tgcctgtgat gttccttatt cttgcaaacg gcggatatga gattgcgaaa
    33781 aacacaaaga ttccaacaag caataagtaa ggaagtactt caataaggtt ctctgtttta
    33841 agtccttttt tcaagagatt tttgagagcc aaattcctca tccttccaga aagagtagac
    33901 tgatcccctg tgagctggaa cagtctactc cagcattcaa aagtgttttt ttagaatcat
    33961 aaggtgctta gttattacat ccttctccaa aacccggaat atcaaagcgg taaggaacgt
    34021 caatcacttc atgaacaagc acatcgtttt ccattctctt aacgatatct gctctgagac
    34081 cctcttccag gttgttcgct acgaatacgt agttgtatgg ggagctctcg aatggaggga
    34141 caacccatgc agcggtgaac ttgtcaaggt aaagctcgta gatctcctgg taaagaccgg
    34201 attccatagc gtccttgtat tccttgggaa cttcgtaacc cctgggcttg tctaggtaaa
    34261 tccagttggt gaatctctct gggtatatcg gaacgagaag accctgtgga tcccagtcgt
    34321 ttggatgaag cgttcttgac atcatcttcc agtcgtagtc gtatggtcct tcctgtccga
    34381 aagtctcgat gtaccagtca acgttttcag gagtaatgaa ggtctgcata gttgacatca
    34441 tcgtttcagg aatggttggt ttccagccgt tcttcatgtc gtatacagta acggcctgtc
    34501 ttccggccat ccagaatccg tagattccag cgacaacgtc gagacttcca tcttggatat
    34561 cgacgaggtt ggacttgtta gcgtcggttg tgacctggaa aacatggtcc aatccggcct
    34621 cttcacaggc aattgccgcg ccaacattca tgcttgtcgc agtgccaaaa ataccgtcta
    34681 tcctgtcacc gaactttgcg atccagtctt tggtaacaga gtatcccttg tctcttatga
    34741 agtctgcata ttcctcaccg ataaccgtga tgttcgggaa gttctccata agagcggatt
    34801 tgtaacctct tgctctgtcg ttacctgctt tggaaccggg gaatccccag agaatgcagg
    34861 tctcgccttc tccgttgaga agtctgccca tttctagtcc gccaagataa ccatcgacat
    34921 aagcgtcagg tccaatgaat tgaccgtagt atgggcctgc atccagcgga gtccaccagt
    34981 cggctatctc gagaatgttt gcgtaataga ctttctctct gttaacgaag ttcgatgcct
    35041 gaataacaga ttcgattgtt ggggagtaac caaatatcat cttgacaccg gcggcagcga
    35101 aagactcgat ttggaacagg aacttttcgg ggctcctgtc gtcgctggct gctatgtaca
    35161 tgtttccaag agcctcgaca gcctcccttg cgcctttgtc aaaggtaagg aagaagtcgt
    35221 ttgccatctg tgaatacgtg cataccattg tctccgttga aagtgcagta acaccaaaca
    35281 aagcaataac aacaattaac agaacactga gctttttcac ctgtaccatc ctccttcaaa
    35341 agaagtactc tcatagatcg aaacattcac acgaatagct ttttttcttt gtgccaacct
    35401 cccttcaaac aaaggaatca aagaatgatt cttatcttgt attcgcctcc gtaaccttcg
    35461 ccgtagtaat cggtcttcaa tgcgtgcagt tgtcctttga aataacggtt tccaacggga
    35521 gtctcccatt tctttccata ggcctcggtt acgtcgccgg tgatttctcc aagttttcca
    35581 aagcgaggca ttaaaacggg ggtagggcca tcaattgcga aaggcgggta atgccatgtc
    35641 cctttgttca aaagaacgcc ttcgttcggt ccaataagga aggctctggt cttttcggct
    35701 tcggggatct tcgatttcgg cgcaagaacg aatatgaact gttttccacc cagagggaaa
    35761 aagaactcaa gagtttcata atgaacttct gtccagttaa ggatttcccc tattggcttt
    35821 ggataaacga aaagcattcc cattgagaat cggtcttctt tgttgtcctt gaaaggaact
    35881 agatcccagt aagccttgat catgctattt tcgactgcgg cttttgcgtc cacgggatgc
    35941 aactcatact tgccttcttc cgtgtcttca tctctaccga tgattctgcc aaaggtactg
    36001 aaatctctca tacttacttc actcagtttt tccagtttta catcaactac tgccataatc
    36061 tctctcctcc attcttagat tcttttgctc acacagtgaa cagaaatcta tttttcagaa
    36121 actgaatgca tggaactcac aaaacaaaaa aaggtgcccg aatctaatca ggcacccata
    36181 acctttatct tcctttgcta agcaatacca accataagta tcgccgcaac tatattcgct
    36241 tttcagatat ctacgagaca attatgcaca gaagaacacc caatacaaaa tccgatctcg
    36301 cgagctctat tagatctaag agtctttaac agcaattatg atagaaaagc tcaatcattc
    36361 gcgtattccg aatggcagga actgcgaacc tagattccgt aatgcaaagc attgtcggct
    36421 tctatgattt cctttgcaac tgcaacgagc ttcaaattcc tgtctctact gatcttctgt
    36481 agcaacttca ttgcctctga ctctctgatt ccgttacggt ccattaaaat ccctttagcc
    36541 ctttctataa gttttctagc ctctagagct tcttcctgct catggagctc ctttttgaga
    36601 agcatgaact ctttgaatcg acccatagca atctcaattg cgggcttgag ctccttctca
    36661 tcaacgggtt ttacgagata gttgaagacc cctgccgagt tcgcgcgctt aacggtttct
    36721 tcgctgctgt aacccgtaac aattatcgag ggtacgagtt tcacttttcc aattctttca
    36781 attgcttcga ttccgcttag tgcgggtagg tttacatcta ctatcagtaa gtctggatcc
    36841 gtttcaagtg ccagtttcac aaatgtctct ccatccatag cctctcctat aactttatga
    36901 ccgaggccaa ttagtgtttc cttaagacct ctcaggacta tgtactcgtc ttctgcaata
    36961 agtatgttta aagaattgtc cataggaatt gactcccctt tcggctaggt ctagcacata
    37021 atcgccaata attcttttat gagcctctcg tgagcaacag gctctctttt ggaatcaaga
    37081 tcttaacctt agctcctctt tctgaggcga tctcaagcgt accgtcgaac tgattctcaa
    37141 caatagatct cgcaagcgaa agccccagtt tctcgtcttt tgctgaaaag gatctgttgg
    37201 gaaatcccgc gccgttgtcc tccacgacta tttccacatt atcgtcaaag ctcttgcagg
    37261 aaatcgatat caacccgcta tcaatatctt taaaagcgtg tttcacacaa ttgtttatca
    37321 gttcgcttat agtcagtgag attccgcttg ctttgtcgta tggaataaag atgtcgtcaa
    37381 gatctttcct tacctggaga gtgccgtcaa ctatgttgaa acgtactata gtatctacaa
    37441 gactcttcag gtttattata cttctgccca gttcatcgtg agaaaggaga tcatgaacgg
    37501 cggctatgct tttcaccctg gaaacgacat tgtccagcag atcctcgact tcgttgtctg
    37561 tctttttttc gaaactcctt ttctgaatat ttatgagact tacaatagtc tgaaggttgt
    37621 tctttatcct gtggtgactt tcctgaagca gcaccgagct tcccataagt ctcgtattat
    37681 ctattgcaag ggccacctgc ctcgagagcg tatgaaatat cttcttctgc ttttcatttg
    37741 gttcatagta tttacgatag cccacctgaa acaaagcggt gtggtgagat ctaacggaga
    37801 ctgggtcgca aacgattgtc tcggtatttc gaatgctctt gaagagcttt tcgaagagaa
    37861 catcgtcaaa attggaaaca aagagcttct tgttgtccat tattcgcata acaacttcat
    37921 cgttgagaac ggcttctatt gactcggttt ctttatgttt tattgtatcc atgattgtcc
    37981 tggtcttttc gtcaaccagg tacacataac acgaatcaga ttccattatc ttgcaggtat
    38041 aattttcaag cattttcaag acatcgttga tttcatagtt cgaagtaagg tactcggccg
    38101 ccctcaaaac tgcatcaatt atatcttcgg tttctatttc ctcggcctcg cgtttcgttg
    38161 aatctgattt cttccagcca agtataagtt tcaaaagctt ctccggcttg tcttttgcct
    38221 cttctgagct tagagttcca caaagcaccc cttcagatag aacacttatt ctgtctgagt
    38281 acttcagtgc ctcgtcccac ttatttgtaa cgtaaagtat tccagtcccc ttccccttaa
    38341 gttcattcag tattctaaga agaaggtcct gagacttgga gccgagatta gcagtgggtt
    38401 catacagaac caccagccgt ggctcataaa tacacagtct cagaagctct ataagcttct
    38461 tctcctcggt agaaagctta tacacgtgag tcttctctga aatcccaagc tcaaatcttg
    38521 caatgagctc tttcacagca cgatttatct ttgaatggtt aatcagcttg ataacattgg
    38581 aatgactcct caacacgtac atgttctccg ccacggtcaa accctctgcg accatcggtt
    38641 cttcaaggag gaacagaaca tctgtcgaac ctccgatctt gattttgttc gtgacatcta
    38701 ttccatcaaa gaagactctt ccggaaatct tcttctcagc cccggtgaga acattaacaa
    38761 caagttcctt tccagcacca tactctccga caagggcgtg aacttcagaa gaggtgactt
    38821 ccagattgat ctccttgaga tcgacattac ccgtgtcatt gctactaagt tctttgatat
    38881 caagaataca ttgttccata gcggatccct cgaattctgc agactttttc ctttcatgct
    38941 gctgcagcaa caataaatag atcacggatc aaaaaagaaa gtcaaacaaa catttgcttg
    39001 ttgagaatga gtctcataat cattctacaa cacaatgtat cgaaactata tgtatgtgta
    39061 ggtttctaca ttttcagagt ctaatctaac tcgaagaagc cttgatcagc tataagagta
    39121 gtaggattcg agactcgcga aaactagtgt tatgtgaagt acaagttttg aagtaaaaac
    39181 atcacagtag agaatgataa taaaatatta attagtgtta aaatttatat gtaaagcaga
    39241 ttgacgagtc tgttcatttt gaacgagatt ctgttcagtc tagctacatt agaattaacg
    39301 tcctgatgct taactggttc ggcttatctg tcaggtcaag gaggaatctg aaatgtctta
    39361 caagcactac tatgttggtg actccgtttt gtcgacgggt gactacgagg ttcacactgg
    39421 tgattgtgtt gagctggcaa gagtagagtc aaagacttat cttggctatt atgataactg
    39481 tcaggatgcg attaggaagg caagtgatat ctatccgaac gtaagcggct gcaaatactg
    39541 ctctcctggt tgtcacagga gataattttc cctaattgcg ggggctatcg attctttact
    39601 gcaacactgt aggttggtac gtgtgcgatc tgaaaaacta ttcgaaaacg ctgcttgtga
    39661 taaaatcagt cgaacctgga tggttatcga taccagatag ttatgaactt tttagaggga
    39721 gagacctatt tgaatgacgg cagaacaaag gttggaatag gttttgcgac ggggcgaagg
    39781 cattttcaga gagttttgcg tagctacgtc tacaactgga aagagtctga actggttgac
    39841 agcagagaac taacgctgaa tctatttgtt gcgtatgatc tgaattacaa gaatacaaag
    39901 aaggcaaatt acaccaagat tagttccggt gtggacgaat tactgttctc caaacacttc
    39961 ataggtcaga aggaagttga agaagaaaag agggagctca ttaagcgcgg gattgtcgat
    40021 tcggaagaag cttctttggt ttttggaaaa ggatatgctt cccaaagaaa tattgttctg
    40081 tacagcgctc taaagaatca catcgattgc ctgctctacc ttgacgatga cgagtatcca
    40141 gttgccgtta cgaacgacat gggctctgtt tcgtggatgg gccagcatct tctgaaaact
    40201 cacttgaact acattgatca ggccgacatc acctacggtt atcactgtgg atacatttct
    40261 ccgatcccct tcatagagtt ttctccagca ctacccgagg atactttcag actcttcatt
    40321 gaggcgatta gcaacgacat agtcaagtgg gagactctca aaagacttat agttgctgga
    40381 ggagtatcgt acgccgatcc gaaaatactg aacagtggtg aagtctttga agttgaagag
    40441 gtaaatcatg ccaaattcat ctctggatcg aatctctgtc taaatctaaa agatccgggc
    40501 agacttttcc ccttctataa tccacctgga gcaagaggtg aagatacttt cttgagtacc
    40561 tgtctgagcg acaggagggt gctcaaagtg ccaagtcacg catttcataa cgcattctca
    40621 acgtacaacc acattttgat gggtgttctc ccaaccagtc tcagacctat aagagccgat
    40681 tcgagagaca tagttagacg tttctacaga tcctgcattg gatggatcag atacaagcct
    40741 ttgcttcttt acataaccga tcgaaacaac tattcgaaga agattgaaga gatgaagatc
    40801 aaccttaggg aaacattacc ttctatttca tcgtactttg gtaccgattc gttctacaac
    40861 atttccaaag agctgaacaa gtatcataag tcggtcgaga aacactatga gcagttcgaa
    40921 agagtaaaag agatttggtc gaagatatcg gattatctcc gcgaagctca ttaaggttct
    40981 tgaatagaag tttaatctga agcttgtatg gaacccctaa agagcaaaga tcagaacttg
    41041 tcgggctttt ctgcctgaag atcgaaaata tgtccgcatt ctctgcaaac gcgcacaata
    41101 accttggatc ccatcgaaag aggctttcct aaaggcataa gggccgcata acctgaccag
    41161 taaccctttc caatgtccag tgagccgcaa tttgggcatt tgtccgttcg aatcatcgaa
    41221 tacctccagt catcatctag aatcg
//
LOCUS       DAOQYI010000016        40255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1132_length_40255_cov_74.212040, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000016 DAOQYI010000000
VERSION     DAOQYI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 40255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40255
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_40255_cov_74.212040"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKL93_06020"
     CDS             complement(<1..352)
                     /locus_tag="PKL93_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ70855.1"
                     /translation="MILSISLIVILSIVVLSLFERICLPGLIGLVLLGIALGPGGMNL
                     IDPSLLSISDEVRLLALIVILMRAGLGLNRRIIKKIGPLALRLSFLPGLFEGFSIMVV
                     AHFLLNLSLIQGGML"
     regulatory      complement(388..463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            514..714
                     /locus_tag="PKL93_06025"
     CDS             514..714
                     /locus_tag="PKL93_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70856.1"
                     /translation="MNTFLAIVGALFLLYLAIRFFPALIMIVAGVGVVLIVIFAMAGL
                     VVFFLPILLVVGLILVIVKLAS"
     gene            789..1610
                     /locus_tag="PKL93_06030"
     CDS             789..1610
                     /locus_tag="PKL93_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNQ70857.1"
                     /translation="MRDYVAEASLLWKGTKHIVVFPEFFATFLYPSFRKLSFEETTAK
                     TLALYAIKNMGLSFNPLKKAFLRDALVIEAYYHDVFQSVAKEFGAYLLAPSILLPIVD
                     TESEKGRFIRGDKLYNLAYFFNPSGKVVARAFKHNLTKAESSIIFSRGEQELNVVHTE
                     IGVIGIAICYDMFFQSEIEKLDASGCEAVLVPSCNFALWKGRLSDSTQERIWFRDGPI
                     KASSGREKIRYLVNPMATGIVGRDRAQGRSSIWYGGRALAVAREFDREEILNVTT"
     gene            1763..2710
                     /locus_tag="PKL93_06035"
     CDS             1763..2710
                     /locus_tag="PKL93_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNQ70858.1"
                     /translation="MRITVVHDTPLDLHHEKMVNSVVNSLEKSFEVDSKPFEIDAVGG
                     LRGTDLIFNLATGMGDTERQVHLPAVLDLLGIPFTGSGTTANALCINKTLTKLIMKAY
                     GISTPEFFSVRPGETADSLPFSPAIVKPVKEGGAKGIWEDSVVESVDDMQKAVSRIHE
                     RFEQAALVERFIEGREFTVGIVGNEVLPIMEIDFSSLPDHLETFYSYRVKQFHGDETN
                     YICPAKISRREETVIREFSMRVFQVLDLRDYCRIDLKIDDEGKIYFLEVNSLPLLVPD
                     YSDIVKMAETRGWSYEDLINRIVASAIRRYGLGERDGRI"
     gene            2697..3080
                     /locus_tag="PKL93_06040"
     CDS             2697..3080
                     /locus_tag="PKL93_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70859.1"
                     /translation="MEEYEEILDNIRQIPVIDTHEHLVQSEDLLQGRDDVLQKFLIHY
                     MSSDLISSGLDSEVLAITRDSERDLVHRWKLIEDYWEFCRHTGYGRALDDSVREIYGI
                     DGIRGSTIEELGEKFRGVNRPGHMR"
     gene            3198..3983
                     /locus_tag="PKL93_06045"
     CDS             3198..3983
                     /locus_tag="PKL93_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ70860.1"
                     /translation="MPPEFDIVGWLEETYAVEIGSLRDWLEVFELELDENISNGIVGL
                     KSVLAYHRPIRFEEVSLDDAATAFRKALEERGRRSPDRNRGLLIPEIVQDYVMHHILS
                     AANERGLFIRFHTGLLEGNRGILSNSNPELLENPFSKYPRVKFDLFHIGYPYWGVTAV
                     LAKTYPNVFIGMCWSHIISPVFARNALSNFLDAVPFNKISGFGGDCAVVDAIYGHLMI
                     ARENIAWIFAEKIGERTLTVSQAVRIAERLLYDIQKEILLNSL"
     gene            4099..4704
                     /locus_tag="PKL93_06050"
     CDS             4099..4704
                     /locus_tag="PKL93_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70861.1"
                     /translation="MKNNLICFCLYGSTSRGTDTHWSDLEMLMITKEEVPKKAFLEGL
                     VLVTTNSITEKRLCGILEEPGVEWPFYAGLLKNLVVLEGDASKPEKYYDLARRVPEEK
                     FRRALKENLSELVFESSGRIFSCIARKRYEDIYCAVIETLLEMKTALCLLNCTHVNHD
                     YFEGLQESFKFKNCQNDISPWQPDYGGAEVHSALQRIPENS"
     gene            complement(4801..6618)
                     /locus_tag="PKL93_06055"
     CDS             complement(4801..6618)
                     /locus_tag="PKL93_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ70862.1"
                     /translation="MLLKKNRFVLLPAVFLILLLSACPFNPPNKVPVWFLPIGDQIVE
                     ETKTLEILLTDHISDEDGDILSFSVTGKGSVVGALYSYTPGIGESAIEFEEEAVYPVT
                     ITADDGKGGTASESFNVTVMSTNRPPNWITIPNKTVQEGSTLTVLLTTYASDPDGDVL
                     TFDILEGPGEINGNNYEYSPSYEEAAVRFEEAAVYSVVLRAVDSKGAEATTSFSVTVT
                     NSNRVPVLSIPSPQYVTAGTTLSLDISTFASDPDGDSLTFSKVAGVGAVAGSTFTYSP
                     LLSDSGTRNATIRANDGNGGQTDQTFDIIINPSGINNPPNTPSNPNPASGALTQPANL
                     TLSWIGGDPDGDAVTYDVYFGTSPTSLVFKTNRSTTTYPTGALSSSTYYYWRIVAIDS
                     KGATKTGPVWSFRTAPTKAFEETFETYSTGTITGSFGKWHYIESGSVPNPFITTISSH
                     GKVIGFYGGTGGYSVVYTSSVKPIKLGYLEYDIRLSSSTAPKWGWIAPNNTNAFTGIG
                     YLGGSQAIICWTNSGGSYHSEKVMNISPGVWYKVKVVFNHTTGNAAVYVDGTYKRSFV
                     YGTVSAGTNGVRMTTSNSFLSYIMFDNISLWATDAGYVP"
     gene            7197..8408
                     /locus_tag="PKL93_06060"
     CDS             7197..8408
                     /locus_tag="PKL93_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ70863.1"
                     /translation="MTSRDRVLKTLKHCEPDRVPIDLGGMRSTGIHVKAYRKLADYLG
                     YCDLPVRVFDVHQMLAFVDEEIRREVHSDAIELKRLNGGFGTRIDSWKGRDIFDDGSR
                     YLFPDGFDPKAKEDGSLVIERDGVEVATMPRGGHYFDRSYFPLAHAGRKEEISALVLP
                     RLTGEEIAFLKAQLTGIRESTDCAVIGAFGGNFLEAGHSMFGYQEFMERLITDRSLME
                     FFLDRLLETYLVDLEKYLSVLGDDIDIIQIGDDYGTQENTAISPRIFRSIFKPRLKIL
                     CDFIHRKKSDLYIFLHSCGSVYTFIPDFIEVGVQILNPVQTNAKNMEPERLKNEFGRD
                     IVFWGGGCDTQHVLPFGTLEDLEEDIRRRIDIFSPGGGFVFSPIHNIQAEVPPEKIFR
                     LFECAYEYGSQ"
     gene            complement(8454..9410)
                     /locus_tag="PKL93_06065"
     CDS             complement(8454..9410)
                     /locus_tag="PKL93_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ70864.1"
                     /translation="MAFDRSIIPLLGAPGARMTGTTLKENLTNAEIQFSSLTKLMEEF
                     QPDGIFFMMDLTVEAEALGLEVGFPEDDNPYVKEHPIKGPDELSSVVNSWSGISGRMG
                     VFAEVADKMARELPGKRGSYVIGPLSLAGELVGVTDLCMKLIEEPDFAESVIDFCSRV
                     VSEYSRALIDHGADMIAVLEPTAVLLSKRQFKRFALPYFERLRSELSKPLIYHICGDT
                     EHIVEPMGASGAYGLSLDSMVDLKAVAEKIPEDVFLIGNIDPVKVFLQSNEEEVERET
                     AKLLEKMKAVPNFILSSGCDIPLETPPENISAFFRAGRNYRS"
     gene            complement(9414..11192)
                     /locus_tag="PKL93_06070"
     CDS             complement(9414..11192)
                     /locus_tag="PKL93_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNQ70865.1"
                     /translation="MKEAVHKVIFLPSQRTVTAKRGEILLDVLRRESQPVSAICGGRG
                     NCGKCKALVAEGSEAFPLTLTEARLLTRDEIAAGYRLSCQFKVMTDVRVKTEDPVLDN
                     ELSKPPLPKALMREVSPLVRLEEFSLSKPNIHDQASDYSRLISALVTEKPRIDRLNLL
                     RNLPRIIREIDYRGRAVMYGSEVIDILPFSDESGIFGVAIDIGTTTLAVYLADLKSGE
                     ILAVRSAPNPQSVYGQDVISRIDYTIKRAEGIDELRAAVLTTLNKLIDDLCKESAVSK
                     ERIYDTSIVGNTTMIHILLGISPERIASSPFIPAFTGGKVFESRELDLEESIPNSKIY
                     IMPGISAYVGSDITAGILSSGFVEDSGNVLLVDIGTNGEMALKKGDRIFTCSTAAGPA
                     FEGGNISQGTIARSGAVDHVWLNGKDIGFSTVNGSKASGICGSGLIDAIAVMIGAGIL
                     TESGRIKGEYFELEGPAGIVRINKRDIREVQLAKAAIRAGVSVLMDRAGIETRDIDRI
                     LLAGAFGNYIDPENALRIGMLPNVPVERVSPVGNAAGLGAVLAVLDSGIRERAENLSN
                     NIYYVELSGRKDFNEIFVDNLALREG"
     gene            complement(11299..11997)
                     /locus_tag="PKL93_06075"
     CDS             complement(11299..11997)
                     /locus_tag="PKL93_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNQ70866.1"
                     /translation="MVEREKNISILKGIPFEIDEASLGESLRIRAGSEEESTLKELVE
                     KVRETANPKAIYRTCFVDCVNGDEVTIDGVRFESRLLSKKLHSVGRVFPFVITSGREL
                     YEYPIDRADFLKIFLWDSLLEHVLHEAAGFMRREISRNNLIENLVWMSPGSGDAEIWP
                     LQQQKELFSLIGNVREEIGVELKESLLMIPTKTISGIAFRSEKDYRSCMVCRRINCHY
                     RSAPYDEKLRNSFE"
     gene            complement(12031..13089)
                     /locus_tag="PKL93_06080"
     CDS             complement(12031..13089)
                     /locus_tag="PKL93_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ70867.1"
                     /translation="MARKPDFSIILNTLKRKDEQGIVPFFELFADDEIMEEVMGYKLA
                     KVEEDPDRYFDQLVGFYKEMGYDYVPFYQAPRFPTPDYIHGEDTATYRRESRKWMNEK
                     GGPIKTLKDLQEADWPKPEEAVDFDLFRKLGDHLPEGMKVIGGASGGPFEHSSFLMGV
                     ENLSMAVYEDPELVNTLIEKIGNVLVGAAKIISSIDCVGAYCFGDDLGYKTSTIFSPR
                     HLRRLVFPWYKEIASVVHANGKPFILHSCGNLTTVMDDIIDAGVDAKHSFEDQIMPVT
                     EVKRRWGKRISILGGIDVDFLCHASIEQVRSRTLATLEQCAPGGGYALGTGNTVANYI
                     PVKNYLAMLEAGNEFNSR"
     gene            complement(13298..14446)
                     /locus_tag="PKL93_06085"
     CDS             complement(13298..14446)
                     /locus_tag="PKL93_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70868.1"
                     /translation="MRKKRLGVIWVCLLASLVLASSSLLAQGDSGYYLGTSANGYQIP
                     RDGGLKLEPVTGKDGWYRITIDFTEENRDPMYDGHYYKVTDGTWNADGCWGVDNYAFQ
                     PAPVKILPDGSVAGLGSIYIKDNCTLTILFDANTKTIYDDSVQAFPTPRIYGDFNKAM
                     GRGADWSMADGEALTLVDQNGDGIYTGFYEIPKYEGSGNGYMMATVLSTKYDTTYYIF
                     GAYEQYLFDGNPAGMGKVSYLKPEKDTIYEFRYDSNSHSTSIVECITDQIVQLPSPVI
                     YGDFNGWNIEGPFAVQFERTEEGTYTVAHKFSEYKGDGDGYMILVCISKKFYNDQWGM
                     RWGAHEQYKLDGQVAGMGEFSYLKPDKDTVYRFTFYPESKITEVEPIQ"
     gene            complement(14483..16252)
                     /locus_tag="PKL93_06090"
     CDS             complement(14483..16252)
                     /locus_tag="PKL93_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNQ70869.1"
                     /translation="MDRSSIYHISEHNYAYATANDTLAVRVRTRKDDVKRILVHYKNL
                     YDHSERISTTEMKKILTDGICDMYEASLVVEEKRFKYYFELFDVVDHIFLTSDGLLDN
                     FESGSFFYYPYINDDDIFGLPAWAEGEIIYQIFVDRFFDGDPSNNPEGTRSWNVLPDK
                     TIESHGGFKGELRRNTYYGGDIEGVIKKLDYIRGLGAKIIYLTPIFKSDSYHKYDIND
                     YFVIDRAFGDKAKAKEMVDEAHRLGLKVLLDGVFNHCGYANRLFMDVIENQSQSKYKD
                     WFCIRSFPVNENRKDYDSFGNVAPSMPRLNTSNPEVIRYVIDVAKYWTEELGVDGWRM
                     DVADEVSHRLWVEFRSKMKRKWPEMLLIGEIWNHASRWLQGSEMDTVTNYKFMRTLHL
                     FSQRTIGSHEFWNRTGANMMLYKTLVNNYLVNLVGSHDTKRCRTTLGSERRHVLAVLV
                     TLAFQGMPLIYYGDELCMEGADDPDNRRPMEWGALETNCALTIREFSRFRSRSEVLKK
                     GKMVPLNTNQERVLAFIREFDGKKLLFAANFGDSPATFSLEGYICRLVLGNAIVDGAK
                     MKIGREDYALLEIDGEFTRSQDI"
     gene            complement(16283..17116)
                     /locus_tag="PKL93_06095"
     CDS             complement(16283..17116)
                     /locus_tag="PKL93_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ70870.1"
                     /translation="MYNVKKRVGRILVHFILLILAVVTIIPFFFVVLISLGKNVIDTS
                     VFFVKELTLDNYSRLFTETRFSNWLINSLLLATVTMILSVIVVSLSACAFSRLRFQGR
                     TKLFNFVLLIQVFPLTLSMVSIFRIFLALGLLNKIEGLIIVDSAFASAGLVLIAKGYF
                     DTIPYSLDEAAMIDGANRFRILTQITLPLAKPMIAVVAIQSFVIAYNEYAIASTVMTQ
                     KLEAMPLAVGLQSMIEGQFGINWSVYCAGAVLGSIPMLVLFYSLQKYFIGGLTEGSVK
                     A"
     gene            complement(17177..19492)
                     /locus_tag="PKL93_06100"
     CDS             complement(17177..19492)
                     /locus_tag="PKL93_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ70871.1"
                     /translation="MKKTWVVVFTFILTGISLLSSESDFAVRIWFSHIDSENQVMADI
                     ASEFTLETGIPVEVVSRRSIFDAPRDLANYAEAYDRPDIVLMQAPDIGNMVKSGFILP
                     VEIDSDLRERFVSVAFEAFKYDGEYYGVGYSVDTSGLVYNKQLIREEDIPKTWEDFFL
                     VAEELTVRDSSGKIVQYGTLINPKDMWFNYPIIKEYGGYYFGVLPDGSCNPYDVGLDN
                     EGMLAYVEKIKELQSKGLTLTNPNATESHISAEFANGRVAMILYGLWNASIYKSMGIE
                     YGISPLPVGKNGKVSKPLATVQGFVINRFTNDLDATKSFLEFILKDENQQRLIEAGNR
                     GDRKTGERNPANISVINSKYIEEDTVLSSLSNIGFNCEPFPNVPEGPIWYNYTSTALR
                     AIFYGDTAGNEVDAQEKLLELAERIRADVALINEIPEKIDIQASHVLIFAAFLAGLIA
                     AVVLLRKATKRNTSQISPLDRTEKLGTKNTMIAWAMLLPLLLLLLVFYVFPIFHNIYL
                     SVTNYSGVNLRDYGIVGLTNYREIFTSGIGGLASMFVWTITFAFMVVTISFIVGTALA
                     VTLDKVGIRVAKVYKLIFILPWVVPSVITLLMWRGMLEDQGLVNQILSLFGINSVPWL
                     SHCLAARASSIIVMSWFSFPYFMVISSSILKSIPKDYFEVARIAGAGRLYIFFKITIP
                     LIIRAIFPMLVMSFIMQFNQFGVYLLTQGGPPGSRLGSPGATDLLITYVFNTAFNTKR
                     YSLAAAYSVIAFCFVAVFAVVSLRIANRKSY"
     gene            complement(19527..20600)
                     /locus_tag="PKL93_06105"
     CDS             complement(19527..20600)
                     /locus_tag="PKL93_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ70872.1"
                     /translation="MFRELSYYKPDLDKTVGMMDKVYRDALNHKVGNVTFRALPKSDV
                     ALYSRFDLTKYNYENDIEQYAKDLCELYRESFEERRNVDDNMVPSVSPVLGIADYSAF
                     VCGDIKFHRDTSWFKPVLREIEDFKSLPKLGTAYWYKNFLNICEEILKISSPAGIPFL
                     RGFFSPLDLAASLRGEAIYTDFYEFPAELHSLLDYCADALIAFAEDVYSLAHRYLAGK
                     KYGMWHIKDCIYMSEDTACMISPKLYRKFCAPYTQRVIDHFGIGHMHCHSRALYLVKE
                     ICSLDKVASIWIATDPNQPRPIENITYLIDSSNGVCLSIDCDRFDEIEGNIEELRKGN
                     FSITLPVETAEEAEQSARDFWEL"
     gene            complement(20655..21485)
                     /locus_tag="PKL93_06110"
     CDS             complement(20655..21485)
                     /locus_tag="PKL93_06110"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ70873.1"
                     /translation="MSDYTTLCKLGFSTNEAKCYIALHKKSPLTGYQVANEAGITRTM
                     VYDILRRLARKGYIEVIEGNPRLYSAVAYKRLVSNIRNDYERRLEELEETLEKISREV
                     VDNEYVLNISGRTEVISLIRKAIIEAKREIYLSIWDSEARLFEDDLKKADDSGVKIYI
                     FSFCKLPFDFGIQYTYGMKDAKENFPRRRIVAVFDRELAIMGEGNEEINEIGITTRNQ
                     MIMQLAIDQILLDIILLHTLRSGGYINKEIQIDDYHKSIDDFFKSISLPKDLPLRIDE
                     "
     gene            22062..23543
                     /locus_tag="PKL93_06115"
     CDS             22062..23543
                     /locus_tag="PKL93_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNQ70874.1"
                     /translation="MKLITKKMFLEYDYCPVRGWIERNEPREFRPTIAEDFRMKEGLD
                     VGRRARSLFPEGILIEEMDPFEASFITERIIGDRESVTLFEPAFISGDFVSRADVVIK
                     EGESLDVFEVKSSLAGSSNTKDYIRDLSYTVSVIESLGHRVNRACLIMVSRMYRKGDE
                     DRKLFEIIDVTDEVSAQKSEIKAKLEPASVILTSTLIPSGSLGYKCRDCEHLRRCFGL
                     ERKLSIFELPRLGADKTDALIERGYFYLEELPREALGDKPLLQRVFRGAKEGRIVFDE
                     PEELREKLNSFKYPIGYLDFETAASVIPLYDGLAPYEGIPYQYSLHIRREVSDVLEHR
                     EFLFDNPSRDESLLLAERLVEDLKDCRTLMAHHASVERNILRWLSNRYKDFPELSEQL
                     ISFSEMFVDSELLVKNHIYHPDFGGSFSIKRLLPALVKGVGYEGLFIHNGDDARYVFV
                     NMALGNYRESEIEKIRRDMLEYCRMDTLAMVEIHGFLCDLSSE"
     gene            23691..24734
                     /locus_tag="PKL93_06120"
     CDS             23691..24734
                     /locus_tag="PKL93_06120"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNQ70875.1"
                     /translation="MKNHETSLPLIQGGMAVGISLDNLAASVANEGGIGVIGTAGIGM
                     TVEGYRSNFRQASIDGLRNTIRRAREKTKGVLGVNIMVALTNYAEMVATAVKEKIDVI
                     VSGAGLPLDLPSYLEEGSETAIIPVVSSLKSATVIFKRWFGRYRYVPDGFVVEGPKAG
                     GHLGYRVEEIFSEESSLEKTLPEIRKFVDRVKLDTGKNIPVIAAGGIFDRDDVERVFK
                     LGASAVQVGTAFVATEECDADYRFKQAFVDARSEDVTIIKSPVGMPGRAIRNKFIEDV
                     ENGKRKPFKCAYQCIKTCNYKEAPYCIAEALLNACKGDLENGFAFCGSEVHRISGVTT
                     VKKVIDRLFGERR"
     gene            24805..25437
                     /locus_tag="PKL93_06125"
     CDS             24805..25437
                     /locus_tag="PKL93_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNQ70876.1"
                     /translation="MESLEKIRKAIIETMPAEAKVATEEALAEGLSAKEILDGALIPG
                     MDEVGKLFREGEYFVPEVLVAAKAMNQCMDLIEPLLVSGGVKKIGKVVAGTIEGDLHD
                     IGKNLVCMMLKGAGFEIIDLGVDVKPEEFVEAAKGAKPDILVISALLTTTMLNMPRVI
                     EELKKAGIRDDVKVLIGGAPVSQTYAEEIGADGYSPDSSAAVELARRLVS"
     gene            25659..26903
                     /gene="rmuC"
                     /locus_tag="PKL93_06130"
     CDS             25659..26903
                     /gene="rmuC"
                     /locus_tag="PKL93_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNQ70877.1"
                     /translation="MLIALIILSIVTLISFLFAIASVRRLSRSIKKNQELQLSLKDLE
                     RDVSDRDEKLKWLTSAKEELESTFKAISSDVLNSSTENFIRQANDKFSGLLKLQKEDW
                     GSQKKDFESLVSPVRDNLEKLEKNVKELESKREGAYKALEEQLKMLSEANTDLRQGVT
                     DLKSALRNRSTRGRWGEIELRRIVELSGMTEHVDFVEQESIDNGRPDMIVKLPNNAFI
                     PVDAKVPLDSYLTALEEEDESLRKQLMGNYAKNVRDTVNRLAGKKYSEAFSRTADFVV
                     MFVPLESAINAAFLADPELFEYAVSRKILIASPVNLVALLKSVAYGWQQYSLTENAER
                     LVQASKELYERFRVFFSHYSRLQGHLKNAVRAYNDGVGSFESRLLPKVREIEALAEVN
                     EKLEEIEEILDEPKSSDLVDRN"
     gene            27176..27871
                     /locus_tag="PKL93_06135"
     CDS             27176..27871
                     /locus_tag="PKL93_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNQ70878.1"
                     /translation="MNEILWFLMLVVNFALITIAYRWWGKTGLYVWVAIAAIIANVQV
                     IKTVSLFWMAATLGNIVYATSFLATDILSENHGKKEARKAVYVGFFSLISVTVIMQIA
                     LAFEPHPSDFSQEHLSVVFGLLPRIALASLAAYWCSQLHDVWAYDFWKKRFPEKRHIW
                     IRNNASTMISQLIDTAVFTVIAFAGVFPAGVWWQIFWSTYILKWVVAVLDTPFIYLAR
                     KMKDDGKIRDLVP"
     gene            28030..28632
                     /locus_tag="PKL93_06140"
     CDS             28030..28632
                     /locus_tag="PKL93_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ70879.1"
                     /translation="MKEREEKLDKYVEKRDFSKTSEPDGREATVEWSKERPVFVIQKH
                     AASRLHYDFRIEVDGVLKSWAVPKGPSTDPSEKRLAVPTEDHPLEYGDFEGNIPEGEY
                     GGGTVLLWDRGSYRNLKEDPESNPLSKQIEDGHITIWLEGHKLRGGYALIRTGKGSKA
                     RWLLVKMNDEEADARRNPTATEPDSVKTGRTLEEIRDSGE"
     gene            complement(28767..28982)
                     /locus_tag="PKL93_06145"
     CDS             complement(28767..28982)
                     /locus_tag="PKL93_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70880.1"
                     /translation="MNLAFLLLWSTLALTMIMISNTADALVLIGVASTVSIHLLKLKI
                     LTAEILVKVREGLKNPEIIDEYPCSND"
     gene            29542..30375
                     /locus_tag="PKL93_06150"
     CDS             29542..30375
                     /locus_tag="PKL93_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNQ70881.1"
                     /translation="MHAVLSKKRIAPDTYDIWLSHPDIYRYAKPGQFLIIRIDEFGER
                     IPLTIASAEKDRVRVIVKAVGKSTYKLCAMHEGESVADIVGPLGNPSEIEKLGTVCVV
                     GGGVGIAPLFPVARALKSAGNEVIGILGAANKDHLIMVEEFKPFLDRLYLSTDDGSAG
                     VKGTVTNPLKEVIADYTPATIWAIGPMVMMKFVSLLASQNDIPCWVSLNPIMVDGTGM
                     CGACRVEVGGEMKFACVHGPEFDGRLVNWDELIKRQYQYREEERLALEKYVHELGEKD
                     G"
     gene            30329..31747
                     /gene="gltA"
                     /locus_tag="PKL93_06155"
     CDS             30329..31747
                     /gene="gltA"
                     /locus_tag="PKL93_06155"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNQ70882.1"
                     /translation="MLLRNTYMNWVRRMVEKFKVRMRELDPSERVNNFEEVALGYTKE
                     EAIAEANRCLQCKHKPCVAGCPVGIDIPGFIKALREGDIEKSTRILKDANNLPAICGR
                     VCPQEKQCEMACVVGKIPGSEPVAIGRLERFVADQNIPLETTVKPSVNKRVAVIGAGP
                     AGLTAAADLAKEGFSVTVFEAFHTAGGVLVYGIPEFRLPKEIVAKEVEFVKSLGVEFR
                     FNQIVGRTIPFEEIKSEYDAVFIGIGAGAPRFMGIPGSNLNNIFSSSEYLTRTNLMKA
                     YLFPMYDTPVKIKDRVAVIGAGNVTMDAARTAKRLGAKEVHVVYRRSEEEMPARIEEY
                     HHAVEEGIVFHWLTLPVEYIGDINNNVTGMKCVKMTLGEPDDSGRRRPIQVEGSEFIM
                     EVDMVIEAIGQTPNAILKAGFSTVRLNKWGNIDADEASGRVDEGIFAGGDIVTGSATV
                     IEAMGAGKRSARAIKSYLLRKR"
     gene            31892..32119
                     /locus_tag="PKL93_06160"
     CDS             31892..32119
                     /locus_tag="PKL93_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70883.1"
                     /translation="MTYFFLDRSDGNLSKKIAVSLVEKAFRTLKGVDLKIRPIHHWLE
                     KRVRAHIFLCMLAYYVEWHLRESWKGMIFTD"
     gene            complement(32386..33822)
                     /locus_tag="PKL93_06165"
     CDS             complement(32386..33822)
                     /locus_tag="PKL93_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ70884.1"
                     /translation="MRSWSLSDKLWLLFAAVLSILFLLLGIVFRWSIRDFFTTEAYRT
                     IEYAQEVRVIELQGGFIPFEDIDLNSLRNVRDVGHIIVFDREFDLIDYYLNRFGTQDS
                     SDKISPSPDSTSRDILISGTIIPEILPAIQSIRENAEKQTSSSERYETLLSGRSLFYV
                     IRNEEISGRDVTMISYMWDTYMNTLSSTLFSRLLLVMAIMTVVSLVFIIVFSRYLTKP
                     LKKLSEDVRMISKRNWDRPISLNRSDEIGELADSIENMRKSLSEQDREQQSMLQFISH
                     ELKTPVMVIRSYAQAIKDGIYPAGDLDSAIDVIDKEIQRMDLRVKDLLFITRLEYLSR
                     HDLEKEEVNLSELLEDTVGRFSFQRDDIDWNLNLKDFKTEVNEEQLAIAIENILSNQI
                     RYAKSRIEVTMAVNEREESLLIRFANDGEKVAETKLEELFKPFNKGVGGENGLGLSIT
                     RRIIELHGGAISIVNEGEFVATIVTLPI"
     gene            complement(33819..34493)
                     /locus_tag="PKL93_06170"
     CDS             complement(33819..34493)
                     /locus_tag="PKL93_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ70885.1"
                     /translation="MGTRINLVEDEKNLNSVLTSYLEKEGWEVKSFKNGSDAFDEIDD
                     SPDIWILDIMLPGIDGFELLREIRKRDSMIPVIFISARDKDIDRVIGLEMGSDDYLPK
                     PFLPRELVIRVKKILERVHHSGGIISVPPYKIDEARRTVTEEVDENQSKLIELTSMEF
                     DLLLLFISGKGKPFSRDQILDRVWGYDHYGSDRAVDDLVRRLRKKMPHFKIETVYGFG
                     YKSVNI"
     gene            complement(34810..35193)
                     /locus_tag="PKL93_06175"
     CDS             complement(34810..35193)
                     /locus_tag="PKL93_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70886.1"
                     /translation="MLVTSHEKRYSDKTEMTLDDLIQKLKDLGLHSLKRVVVSEIEGT
                     DSHLVTDNRVIDPDDLEETLRSWEDESITEVMLLDMTPVHKYHNTSPSATEVIYEPPE
                     FSIQVTRVSNGVETIVVAVKSVEPG"
     gene            35380..36429
                     /locus_tag="PKL93_06180"
     CDS             35380..36429
                     /locus_tag="PKL93_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ70887.1"
                     /translation="MKGIITVLFILCAVILASEPLSIGFIYVGSADDGVWTEKHDEGR
                     RYLEKTFGSQIETSFIENVTEGEQDLEVLRSYAERNVKLLFSTSFGFMDDVMEIAKNY
                     PDTIFMHCSGYKTAENVGTYFGRIYEPAYLTGLIAGEMTKSNIIGYVATFKIPEVIRG
                     INAFAIGIAKVNPDARVHVIWTETWFDPSSEEEAADALLDLGADVIAQSQDSPAAVQA
                     AGQRDVYAIGYNTDMSAFSPDTFLTSPVWNWGVFYERVVKEVMEGKWTSYQYWGGIED
                     SVVDIVMSNLVPADLQKLVNEERSRIIEDDYHPFEGPLQDQDGKLRYSEGEKPTDEEL
                     LTMDWFVSNVIGSPK"
     gene            complement(37504..38790)
                     /locus_tag="PKL93_06185"
     CDS             complement(37504..38790)
                     /locus_tag="PKL93_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ70888.1"
                     /translation="MKSVTGEGLSFRKAKRRWTLFFTLIVVSLVSVLSLFIFVLSLRL
                     ERGAEITALNRVADRIESRIGRAPLIALERMLRNYSGNLDLFTAENEILEFVTPDGES
                     VLQLGGRIKSSMPLVEGTSKAKVVDYNDSELEYNILTRAIRNMAGQPVFFLRVGRSTE
                     GLSEKSTSLLFSLILLIVLVAAISWIVGMALAGYVLTPLRESYDRLQRFTADASHELK
                     TPLTVMRLSVDLLKSRPLEGEARDKIDAIDGATRNMQNVVNQLLLQAKAHNDSKAVMS
                     SQDVKLLEFLEEMKEYHRAFAESKGVRIIVETDENLIVKTYLDKLKVAVAAVLENAIK
                     FTDEKSDVIVRAFEREGSLFIQIGDSGPGIADPEKERIFDRFYKIDESHNSSGSGMGL
                     SIAKEMVGSLEGEITVENRPGGGSVFEIRIPLRKHK"
     gene            complement(38771..39448)
                     /locus_tag="PKL93_06190"
     CDS             complement(38771..39448)
                     /locus_tag="PKL93_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ70889.1"
                     /translation="MKILIVEDERSLGRLLKESLEREGFVAELAVDGEEGLYMALNGP
                     FDVIVLDILLPKLTGWQVLEKIRDSGSRIPVLMLTALDSIEDKVRGFDLGADDYLPKP
                     FDIRELVVRIQSLLRRAQFGGAPSRVLRVGDLELDMSLKTASRGNEKIELRKKEYQIL
                     EYLMLNSGRVVPKSELEEHLWNEDDELWSDVIRSHIKNIRKKVDGGRRKKLIKTVRGM
                     GYEISDR"
     gene            complement(39862..40164)
                     /locus_tag="PKL93_06195"
     CDS             complement(39862..40164)
                     /locus_tag="PKL93_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70890.1"
                     /translation="MTRSAGYRLQGLGSREENWQSIQGFNATEEVVQAAVSGSPSLCH
                     PGLDPGSALLKTVAGGSALVVGKSRRTRCPLNSSPFSEKNGCKFQVASCEKKKTFF"
BASE COUNT        10567 a         9345 c         9498 g        10845 t
ORIGIN      
        1 ctagcattcc gccttgaatg aggctgagat tcagcaggaa gtgagccact accataattg
       61 agaaaccttc gaagaggcca ggaagaaaac taagacgaag agccagggga cctatcttct
      121 taatgattct cctgttcaat ccgagaccgg cacgcataag tatgactatc agcgcgagca
      181 gccggacttc atcggaaatt gagagcaatg atgggtctat gagattcatt ccacccggtc
      241 ccaaagctat cccaagcaag accaggccta tcagaccggg aagacaaatt ctttcaaaga
      301 gggaaagtac cacgatcgag agaatcacta taagggagat gctaagaatc atatgttcct
      361 ccagagtcaa gcaaaaccag gctacgaatt ttggtagaag ctatcagctg gtggcggttc
      421 tggaggattg ttctccaagg caagcttcat tgcctagaga attatatagt ttatgcgtta
      481 tactgtcaat gaccagtgaa cggaggtgat ttcttgaaca cttttctagc cattgttggc
      541 gcattgttcc tgctttatct tgcaattagg ttcttccctg cgctgataat gattgtagcc
      601 ggcgtaggag tggtattgat agtgattttt gccatggcag gtcttgttgt tttcttcttg
      661 ccgatcttat tggttgttgg tctaatactg gttattgtta aacttgcgtc atgaaagaca
      721 ctgattttac tgcaatacag gtctggtttg atcaggattc atttggagaa gaggcatttc
      781 gcagaaagat gagagactat gtggccgagg cttctctgtt atggaaggga acgaaacaca
      841 ttgtggtctt ccccgagttt tttgcgacct ttctttaccc atccttcagg aagctgagct
      901 tcgaggagac gacggcaaag actcttgcct tatatgcgat taagaacatg ggactttcct
      961 tcaaccccct caagaaggct tttctgaggg acgctcttgt gatcgaagct tactatcatg
     1021 atgtctttca aagcgtggca aaggaatttg gagcctatct tcttgcgcct tcgatactgc
     1081 ttccgattgt cgacacggaa tcggagaaag ggagattcat ccggggcgat aagctataca
     1141 acctcgccta cttcttcaac ccttcgggaa aggttgtagc aagggctttc aagcacaatt
     1201 taaccaaggc cgagagttcc attatctttt ccagaggaga gcaagagctt aacgtcgtgc
     1261 acacggagat aggagtaatt ggcattgcaa tctgctacga catgttcttc cagagtgaaa
     1321 tagagaaatt ggatgcctca ggttgtgagg cagttcttgt tccttcgtgc aactttgccc
     1381 tgtggaaggg aagactgagt gactcgacgc aggagaggat atggtttcgt gacgggccga
     1441 tcaaggcatc atcaggtaga gagaagattc gctatcttgt aaatccaatg gcgactggaa
     1501 ttgtcggcag agatagggcg caggggagat cttccatatg gtacggagga agggcacttg
     1561 ccgttgccag agaattcgat agggaagaaa tactaaacgt tacgacttaa ggcgatgcgc
     1621 tttatcagta acacatatag aagtccacca tatcttcgtg acgggatttc cctaatactg
     1681 atactcattt agaagtcgaa aatctggagt atgtataatt cttttgtagt gataattctg
     1741 agatatacca aggaggaatg ttatgagaat tactgtcgtt catgacacgc cgctcgatct
     1801 tcatcatgaa aagatggtca atagtgttgt gaactcactc gagaagagtt tcgaagttga
     1861 ttcgaaacca tttgagatcg atgctgtcgg cggacttaga ggtaccgatc tcatattcaa
     1921 tctcgctacc ggtatgggcg atacagagag acaggtccac cttccggccg tgctggatct
     1981 tctgggcata cccttcaccg gttcgggcac tacggccaac gctctttgta taaacaagac
     2041 gctcacaaag ttaatcatga aggcctatgg aatatccact cccgaattct tttcggtacg
     2101 tcccggagaa actgccgaca gcctgccgtt cagtccggcg attgtcaaac ccgttaagga
     2161 aggcggcgca aagggtatct gggaggactc ggttgtggaa agcgttgatg acatgcagaa
     2221 agcggtttca cgtattcacg agagatttga acaggccgct ctcgtcgaga gattcattga
     2281 aggaagagag ttcacagttg ggattgttgg caatgaagtt cttccaatca tggagataga
     2341 tttctcgtca ctgccagatc accttgaaac cttctattcc tacagggtga aacagttcca
     2401 cggtgacgag acgaattaca tctgtcccgc gaagatctca cggagagagg aaacagttat
     2461 aagagagttt tcgatgagag tcttccaggt gctcgatcta agggactatt gtcgtatcga
     2521 tttgaagatc gacgacgaag gtaagatcta cttcctggag gtcaattccc ttcctctcct
     2581 tgttccggac tactccgata tcgttaagat ggccgagacc agagggtgga gctacgaaga
     2641 tctcattaac aggatcgtcg cttcagcaat ccgtagatac ggactcgggg aaagagatgg
     2701 aagaatatga agagatccta gataacataa ggcagatccc ggtgatcgac acacatgaac
     2761 acctggtcca atcggaagat ttgctccagg ggcgcgacga tgttcttcaa aagtttctca
     2821 tacactacat gagctccgac ctgatctctt cggggctcga ttcggaggtt ctggccatca
     2881 ctagagatag tgagagggat ctcgtccacc gatggaagct aattgaagat tactgggagt
     2941 tctgcaggca caccggttac ggcagggcgc tagatgattc ggtaagagag atatacggga
     3001 tcgacggaat aaggggttca acaatagagg agctgggaga gaaattcagg ggagtaaaca
     3061 gaccggggca catgagatag atcctgaagg atttatgtaa catagaactc gcgataattg
     3121 atccatggac cggccgattc gagtgcgaca ggagcctttt cagaagggtc tggcaaccgc
     3181 aaaactacat aattcccatg cctccggaat tcgacatagt aggatggctg gaggagacct
     3241 acgccgtaga aatcggatcg ctcagagact ggctcgaagt cttcgaattg gagttagacg
     3301 agaatatttc gaacggcatc gtcggtctga agagcgttct ggcctatcac aggccgatta
     3361 gattcgaaga agtaagtcta gacgatgccg caactgcgtt tcgtaaggcc cttgaagaaa
     3421 ggggaagaag aagtcccgat cgtaacagag gcctcttgat tcccgaaata gtgcaggatt
     3481 atgtgatgca tcacatactt tccgcggcaa atgaacgggg tcttttcatt cggtttcaca
     3541 ccgggcttct cgaaggtaat cgtggaatac tgagcaattc taatcccgaa ctactagaaa
     3601 atcccttttc aaaatatccg cgcgtgaaat tcgatctctt tcacattgga tatccttact
     3661 ggggagtcac tgcggtcctt gccaagacct atccaaacgt cttcatcggc atgtgctggt
     3721 ctcacatcat atcgccagtt tttgcgcgga acgctttgag caattttctt gacgcggtgc
     3781 cgttcaacaa gatatccggt tttggcgggg actgtgcagt cgttgacgcg atctacggcc
     3841 atctcatgat cgcgagagaa aacatcgcct ggatttttgc ggagaagata ggagaaagaa
     3901 cccttactgt ttcacaggcg gtaagaatcg cggaacggct tctctatgac attcagaaag
     3961 aaatcctcct taacagtcta tgatctgtga agaaatctca gagaggacgg ttttggagaa
     4021 taagaaggtt gagataactt gagacatgag gagaggtaca acctggctct ggagatttcc
     4081 gggaggcttt cgagagagat gaaaaacaat ctgatttgct tctgccttta tggttctacc
     4141 tcaagaggga ccgatactca ctggtctgat ctagaaatgc ttatgataac aaaagaagag
     4201 gtccccaaga aagcctttct ggaaggcttg gtcctggtca ctacaaattc gataactgaa
     4261 aagagactct gcggaatact cgaggaaccg ggagttgaat ggccattcta tgctggactc
     4321 ctaaagaacc ttgtcgtact ggagggcgat gcatcgaagc ccgagaagta ttatgatctc
     4381 gctcgccgtg ttcctgaaga gaagttcagg agagccttga aggagaatct ctcggagctc
     4441 gtattcgagt cgagcgggag aatcttctcc tgcatcgcaa ggaagagata cgaggatatt
     4501 tactgcgcag tgatagagac cctgctagaa atgaaaaccg ctctttgcct tttgaactgt
     4561 actcacgtta accatgacta cttcgaaggg ctgcaggaga gtttcaagtt caaaaattgc
     4621 cagaacgata tttcgccctg gcaaccagat tatggaggag cagaagtcca ttcggcatta
     4681 caaagaattc cagagaactc atgaggaact acctctatct tctcaagagc gaggagatca
     4741 cttagagtca atcttgggaa ttcgctattt cacctcgcat cagtagaaga gggtttctca
     4801 tcatggcaca taccctgcat ccgttgccca caggctaata ttgtcgaaca ttatatatga
     4861 caggaaacta ttgctcgttg tcatcctgac tccatttgtt ccggcagata ctgtgccata
     4921 gacgaaactc cttttatatg tgccatctac atatactgcc gcgtttcccg ttgtgtgatt
     4981 gaagacaacc ttcactttgt accaaactcc aggtgagata ttcattactt tctcggaatg
     5041 gtaagaacca cctgaatttg tccagcagat tattgcctgg cttcctccta agtatcctat
     5101 tcctgtgaat gcattagtgt tgtttggcgc aatccagccc catttgggtg ctgtggagga
     5161 actcaacctg atgtcgtatt ctagatagcc aagctttatg ggtttcaccg atgaagtgta
     5221 gaccacggag taacccccgg ttccaccgta gaagccgatg actttgccgt gactgctaat
     5281 cgttgtaatg aaaggattgg gcacactacc agactcaatg tagtgccatt tcccgaatga
     5341 acctgtaata gttcctgtag aatatgtctc aaaagtctct tcaaaggctt ttgttggcgc
     5401 agtacggaaa ctccagacag ggccggtctt tgtggctcct ttactgtcta tcgcaacaat
     5461 gcgccagtag taataagttg aactgctcag tgcaccggta gggtaagtag tcgtactcct
     5521 gtttgttttg aacacaagag acgttggaga ggtaccgaaa tacacgtcat atgtaaccgc
     5581 atcgccgtca ggatctccgc cgatccaaga gagtgtaaga ttggctggct gtgtaagtgc
     5641 cccactggca gggttgggat tcgaaggggt attcggtggg ttgtttattc cacttggatt
     5701 gatgattata tcgaatgttt ggtcggtttg gcctccattc ccatcatttg ctctgatcgt
     5761 cgcgtttctt gttcctgagt cacttaacaa cggagaataa gtgaaggtgc taccggccac
     5821 agctccaaca ccagcgactt tggagaaggt aagcgaatct ccatcgggat cacttgcgaa
     5881 tgtggagatg tccagagata atgtcgttcc ggctgtaaca tattgtgggg aagggatcga
     5941 taatacggga actctgttac tattggtaac ggttacagaa aaacttgtcg tcgcctctgc
     6001 tccttttgag tcaacagctc ttagcacaac actatagact gctgcttctt caaaccttac
     6061 cgcggcttcc tcataggagg gagagtactc atagttgttt ccgttgatct caccgggacc
     6121 ttcaagaata tcgaatgtaa gaacatctcc atctgggtcg ctggcgtagg ttgtaagcag
     6181 aactgtaaga gtggaaccct cctgcacagt tttatttgga atcgttatcc agtttggagg
     6241 tctgttcgtg gacataacgg tgacgttgaa gctttcactt gccgttccgc cctttccgtc
     6301 gtctgcagtg atcgtaactg ggtataccgc ttcttcctcg aattcaattg cagattcgcc
     6361 gatgcccgga gtataggaat atagagcgcc aactacgctt ccctttccag ttaccgagaa
     6421 gcttaaaatg tcgccatctt catctgaaat gtggtctgta agcagaattt ccagagtctt
     6481 ggtttcttcc acaatctgat ctcctatcgg aagaaaccaa acaggaactt tgtttggcgg
     6541 gttgaatgga cacgcagaaa gcaaaaggat aaggaaaact gctggcagca agacaaaacg
     6601 gttctttttc aaaagcattt tgaacaccgc catgctttgc cattccagtg gcatatgaag
     6661 tcagcatctc tattgtttga gtctacttca cgactgaacg acattatctt tcgattagaa
     6721 gattaagaag aataagaatg tgagctgaat ttgcttcgga agagtcacat gtatttcgtt
     6781 aataaaccca ctaggttaac taaataagtt tgaaattaaa agcaaatcga catgtacatc
     6841 accgaatagc gaatgatatt ttgagacgac tccagctatt gccaaaaaga gcgagaagag
     6901 actgatagag ccttcgctat acttgatcgg ctgagcttgg ccaaattgat caaaatcact
     6961 atattgcttt tctatttaga tttgagctgc tagggtcgat ttgcaataat cagggagttg
     7021 ctgtacgttc ttcagaagat gtctcagaaa ctgaccgctc attcgttcct gctactatgc
     7081 gcttagagat gaaccctcct tgtatatcct ggagtagtga cgcaatatag tctatctcag
     7141 gcataattga ttcgtggaat ctcaggggga caaagattta aaaggagagg aaccagatga
     7201 cttcacgaga tagagtgttg aaaacgttga aacactgcga acctgatcgt gttccaatcg
     7261 atctgggagg aatgaggtcg accggcattc atgtcaaggc ttacagaaaa ctcgccgact
     7321 atcttggtta ctgtgatctt ccagtaaggg ttttcgatgt tcatcagatg cttgcctttg
     7381 tggatgagga gatcaggaga gaagtccatt ccgatgcgat tgaactcaag agactgaatg
     7441 gaggctttgg aactcgaatc gattcctgga aagggcgaga catcttcgat gatgggtcga
     7501 gatacctctt tccagatgga ttcgacccga aagcgaagga ggacggttcg ctcgtaatcg
     7561 agagggacgg tgtcgaagtc gcgacaatgc ccagaggcgg ccactacttc gacaggagtt
     7621 actttcccct ggcccatgcc gggagaaaag aggagatctc ggcccttgtt ttgccaagat
     7681 tgaccggcga ggaaatcgcg tttttgaagg ctcagctcac gggaatacgc gaatcgacag
     7741 actgcgccgt gatcggggct ttcgggggga acttccttga ggccggccat tcaatgttcg
     7801 ggtaccagga gttcatggag aggctcatta ccgatcggtc tctcatggag ttctttcttg
     7861 accggcttct cgagacctat cttgtagatc tggagaagta tctctctgtt ctgggagacg
     7921 atatagatat tattcagatc ggcgacgatt acggcaccca ggagaacacc gcgatttcgc
     7981 cacgaatttt cagatctatc ttcaaaccta gactcaaaat cctgtgcgac ttcattcaca
     8041 ggaagaaatc cgatctttac atctttcttc actcctgcgg gtctgtctat accttcatac
     8101 cagatttcat agaagtcggt gttcagatac tgaatccggt tcagaccaac gcgaagaata
     8161 tggagccgga aaggcttaag aatgaatttg gaagagatat cgtcttctgg ggagggggat
     8221 gcgatacaca gcacgtcctg cctttcggaa cactagagga ccttgaggag gatattcgcc
     8281 gccgcatcga cattttttct cctgggggag gctttgtctt ttctcccatc cacaacattc
     8341 aggccgaagt accacccgaa aagatcttca gacttttcga gtgcgcatac gaatacggtt
     8401 ctcaataata cccgctgaag aaactgctct tgttctagac ttgagacgtt ctgctacgaa
     8461 cgataattcc tgccggctct gaagaaggcg gagatgttct ctggaggtgt ctccagtggg
     8521 atgtcacagc ctgaactcaa gatgaagttg ggaacggcct tcattttctc gagaagtttt
     8581 gcagtctctc tttcgacttc ctcttcattt gactgaagaa agaccttaac cggatctatg
     8641 ttgcctataa gaaaaacatc ttcgggaatt ttctccgcta cagccttcag gtccaccatg
     8701 ctgtcaaggc tcaatccgta ggcgccgctg gcgcccatag gttctactat gtgttccgtg
     8761 tctccacaga tgtgataaat caagggtttt gaaagctcgg atcttagcct ttcaaaatag
     8821 ggaagcgcaa aacgtttaaa ctgtctctta gacaaaagca ccgccgtcgg ttcgagaacg
     8881 gctatcatat ctgcgccgtg gtcgatcagc gctctcgagt attcacttac aacccttgag
     8941 cagaagtcaa ttacgctctc tgcaaaatcc ggctcttcta tcagcttcat acagaggtct
     9001 gtaaccccaa cgagttctcc tgctaggctg agcggaccga taacgtagct gcccctcttt
     9061 cccggcaatt ctcttgccat tttatccgca acctctgcga agacccccat tctcccggat
     9121 attccgctcc aggagttcac cactgaggaa agctcgtccg gtccctttat tggatgttcc
     9181 ttcacgtagg gattgtcatc ctcggggaag ccgacttcca gtcccaacgc ctcggcttcg
     9241 accgtcaggt ccatcatgaa aaagatcccg tctggctgaa actcctccat aagcttggtc
     9301 agagagctaa actggatttc cgcatttgtg agattctctt tgagcgtcgt tcccgtcatt
     9361 cttgcgcccg gcgcgccgag caaaggtatg attgatctat cgaaagccat ttctcatccc
     9421 tccctcaagg caagattgtc aacgaagatc tcgttgaaat cctttctgcc agatagttca
     9481 acgtagtata tgttgttgct aagattttcc gctctctctc gaatcccgct gtccagaact
     9541 gctagtactg ccccgagacc ggccgcattt ccgacgggtg aaactctttc gactgggaca
     9601 ttgggaagca tgcctattcg cagagcattc tcgggatcga tgtagttccc gaaggctccg
     9661 gcaagcaaga tcctgtcgat gtctcgtgtc tcgatgcctg cccggtccat caaaacggat
     9721 actcccgctc gaatggctgc ctttgcaagc tgcacttcac gaatatctct cttgtttata
     9781 cgcactatac ccgcaggacc ttcaagctcg aagtactctc ctttgattcg tcccgattca
     9841 gtaagaatac ccgcacctat cattacggca atcgcgtcta tcaatcccga accacagatt
     9901 ccagaagcct tcgaaccatt caccgtactg aacccgatgt cttttccgtt gagccacaca
     9961 tgatcgaccg ctcccgacct ggcaatagtc ccctgactta tgttgcctcc ttcgaatgca
    10021 ggacctgccg cggtggagca ggtgaagatt ctgtcgccct ttttgagggc catctctccg
    10081 ttggttccga tgtctacgag gagaacgttg ccggaatctt cgacgaatcc cgacgagaga
    10141 ataccggccg ttatatccga gcctacgtag gcagagattc caggcattat gtagattttc
    10201 gaatttggaa tggactcttc taggtcgagt tcccgtgatt cgaatacctt tcctccggtg
    10261 aatgccggta tgaaaggtga ggaggcgatt ctctcggggc ttattccgag aagaatgtgg
    10321 atcatcgtcg tgtttcccac tatcgaagtg tcgtagatcc tttccttcga tactgcgctt
    10381 tccttgcaaa gatcgtcgat cagcttgttc agtgtggtaa gaactgccgc tctcagttca
    10441 tcgattcctt cggctctctt aattgtgtag tctatcctgg aaatgacatc ctggccatat
    10501 acagactggg gatttggggc agatcgaaca gcaagaatct ctcccgactt gagatccgcc
    10561 agataaactg cgagagttgt cgtgccgatg tcgatagcga caccgaagat cccactttcg
    10621 tcagagaaag gtagtatgtc tattacttcg cttccgtaca tgacggcccg gcctctgtaa
    10681 tctatctccc ttatgatgcg ggggaggttt ctaagaagat tcagtctgtc aatccggggt
    10741 ttttctgtaa cgagagcact gataagcctt gagtagtctg aagcctggtc atggatattc
    10801 ggctttgaaa gagaaaactc ttcgagtcta accaatgggg atacctctcg catcaaggcc
    10861 ttcggaagag gtggtttgga aagttcatta tccaacaccg gatcctccgt tttgactctg
    10921 acatcggtca tgactttgaa ctggcagctc agcctgtatc cggccgcaat ctcgtcgcgt
    10981 gtcagaagtc ttgcctcggt aagggtgagc gggaaggcct cgcttccttc agctacaagg
    11041 gccttgcact ttccgcagtt cccccttccc ccgcatatgg cacttactgg ctgcgactcc
    11101 cttctcaaaa catcgagcag aatctcgcct ctctttgccg tcaccgttct ttgcgaaggc
    11161 aggaatatga ccttatgcac agcttctttc attatttatc ttctctcctc atcggtatct
    11221 tcgattccat ctagcctatc ccttgactcg agagctacac tgcagttggc tctttcctgg
    11281 cggcgagaat gggccattct attcaaaaga gtttctcagc ttctcatcat atggggcgct
    11341 tctgtaatga cagttaatcc ttctgcaaac catgcaactc ctgtaatcct tctcggatcg
    11401 aaaggcaatc cccgaaatcg tcttcgtcgg aatcatgaga agagattctt tcagctcgac
    11461 cccaatttcc tccctcacat ttccaatcaa ggaaaacaac tccttctgct gctgtaaagg
    11521 ccagatctcc gcatcacccg aacccggaga catccagact agattctcga tcaggttgtt
    11581 ccttgaaatc tcccttctca tgaatccggc tgcctcatgg agtacgtgct ccagcaagct
    11641 atcccagaga aagatcttga gaaagtctgc gcgatcgatc gggtactcgt aaagttctcg
    11701 tccggaagtt atgacgaacg ggaagactct gccaaccgaa tggagttttt tcgacaggag
    11761 acggctctcg aaacgaaccc cgtctatcgt aacttcgtcg ccgtttacgc agtcgacgaa
    11821 gcaggtcctg tagattgcct ttgggtttgc agtctcacgg actttctcga cgagctcttt
    11881 gagagtagac tcttcctcag aaccggctct gattcgcagg ctttcaccaa gcgaagcctc
    11941 atctatttcg aaaggtatgc ctttcaagat tgagatgttt ttctcacgtt ctaccatgaa
    12001 ccgaccgaat cggaaaccgg ttcaaagtat ctagcgagag ttgaattcat ttccggcctc
    12061 cagcattgcc aggtagtttt tcaccgggat atagttggcg acagtgttgc cggtgcctaa
    12121 agcatacccg cctcccggag cgcattgctc aagagtagcg agggttcgac tcctcacttg
    12181 ctcgatggag gcgtggcaca gaaaatcaac atctattcct cctagaatcg aaattctctt
    12241 gccccatctt ctcttcactt cggtaacagg cattatctgg tcttcaaagg agtgttttgc
    12301 atcgacaccg gcgtctatta tatcgtccat cacagtggtt aagtttccgc aactgtgcag
    12361 gataaagggt ttaccgtttg catgtaccac gctggcgatt tctttgtacc atgggaagac
    12421 cagtcttctg agatgtctcg gagagaagat ggtcgatgtc ttgtatccga gatcgtctcc
    12481 aaaacagtat gctcccacgc agtctatcga agagattatc ttcgcggctc cgacaagaac
    12541 attgcctatc ttctcaatga gagtgttgac cagctcggga tcctcgtaaa cggccatgga
    12601 gagattctcg acacccatca ggaagctgga gtgctcaaat ggcccaccgg aagcacctcc
    12661 aattactttc attccttcgg gaaggtggtc tcccagctta cggaaaaggt cgaaatcaac
    12721 agcttcctct ggcttcggcc agtctgcttc ctggagatct ttcagggtct tgatagggcc
    12781 acccttttca ttcatccact tcctgctttc tctcctgtat gtggccgtgt cttcaccatg
    12841 gatgtaatcg ggagttggaa agcggggagc ctgatagaag ggaacgtagt catatcccat
    12901 ttccttatag aaaccgacca gctggtcgaa gtatctgtcg ggatcctctt ctacttttgc
    12961 aagtttgtag cccataactt cttccattat ttcatcgtcg gcaaagagct caaagaaagg
    13021 cacaatacct tgttcatctt ttcttttgag tgtattcagt ataatggaaa aatctggttt
    13081 cctggccatc tacctatctc ccctttcaat gtgcggctac tacgaatttt acatcaacct
    13141 aagagattct cgtcttaaat gcgcaaagtc caccggtatt gacaaaaaga ttgaatcaat
    13201 ggggcgcttc aactatgaaa taggcgattt ttcagcgccc cgatctggat aaagtatcag
    13261 cgttagtaag aaaaaggcgt accttttcag cagcacctta ttgtatcggc tcaacctctg
    13321 taattttcga ctcggggtaa aaggtgaacc gatagacagt gtctttatcg ggtttcaaat
    13381 aactgaattc tcccataccg gcgacctggc catccagttt gtattgttca tgggcgcccc
    13441 atctcattcc ccactgatcg ttatagaact tcttcgaaat acacacaagt atcatatagc
    13501 catctccatc gcccttgtac tcggagaact tgtgagcaac cgtgtaagtg ccttcttcgg
    13561 tcctctcaaa ctgaactgca aacggtccct ctatgttcca gccgttgaaa tcaccgtata
    13621 tcactggaga cggaagttga actatctgat cggtgataca ctcgacaatg cttgtagaat
    13681 gactatttga gtcatacctg aattcgtaga tagtatcctt ttcgggtttc agatagctaa
    13741 cctttcccat tccggcagga tttccgtcga acaggtattg ttcataggcg ccgaatatgt
    13801 agtaggttgt gtcgtatttc gtagataata ccgttgccat catatatcca tttcccgatc
    13861 cttcatactt cgggatctcg tagaagcctg tgtaaatacc gtctccgttt tgatccacga
    13921 gtgtaagagc ctcaccgtct gccatactcc aatcggcgcc ccgtcccatg gccttgttga
    13981 aatccccgta gattcttgga gtcgggaaag cctggactga gtcatcatag atcgtctttg
    14041 tgtttgcgtc gaagaggatc gttagtgtgc agttgtcctt tatgtagatt gaacccaaac
    14101 ccgcaacact gccatctgga aggatcttaa ccggtgcagg ctgaaaggca tagttgtcaa
    14161 cgccccagca gccatctgcg ttccatgtgc cgtccgtgac tttgtaataa tgaccgtcat
    14221 acattggatc cctgttttct tcggtgaagt cgatagttat ccggtaccaa ccgtcctttc
    14281 cggtaacagg ctcgagcttg agcccgccgt ctcttggtat ctggtaacca ttggcgcttg
    14341 ttccgaggta ataaccgctg tcaccctgcg caagaagtga agatgacgca agcacgaggc
    14401 ttgccagcag gcaaacccaa atcactccta atctcttctt tctcattgct acacctccct
    14461 ggttgatcat ctcagttgca acctagatgt cttgactccg agtgaactcg ccatctatct
    14521 caagtaatgc ataatcctct ctccctatct tcattttcgc tccgtcgaca atagcgtttc
    14581 ctaaaacaag acggcagatg tagccttcta gcgaaaaagt agctggagaa tctccgaagt
    14641 ttgctgcaaa gagcagtttc tttccgtcaa actctcttat gaatgctaga actctttcct
    14701 gatttgtgtt caagggtacc atcttccctt ttttcaggac ttcgcttctt gatctgaacc
    14761 gggagaattc tcgaatagtt agagcacagt ttgtttccag agctccccat tccataggtc
    14821 ttctgttatc tggatcatct gcgccctcca tgcagagttc atctccgtaa tagatcaggg
    14881 gcataccttg aaaagctaga gttactagca ccgccagtac atgtcgtctt tcgctgccga
    14941 gtgtggttcg gcaccttttc gtgtcgtgac ttcctaccaa attgaccagg taattgttca
    15001 cgagagtttt gtacagcatc atatttgccc cggttctgtt ccagaattca tgagaaccga
    15061 tcgtcctttg ggaaaagaga tgaagcgttc tcatgaactt gtagttagta actgtatcca
    15121 tctcgcttcc ctgaagccac ctcgaagcat gattccatat ttctccaatg agtaacattt
    15181 caggccattt ccttttcatc ttacttctga attcaaccca tagcctgtgt gagacttcgt
    15241 cggcaacgtc catcctccat ccatctactc cgagttcttc agtccaatat ttcgctacgt
    15301 caatcacgta ccgtatcact tccgggttac tcgtgttaag cctcggcatt gaaggggcta
    15361 cgtttccgaa gctgtcgtag tcctttctgt tttcatttac agggaaactt cttatgcaga
    15421 accaatcctt gtatttggat tgcgattgat tctctattac atccatgaaa agccggtttg
    15481 cataaccaca atggttgaat acaccgtcga gaaggacttt cagaccgagt ctatgagctt
    15541 cgtccaccat ttcttttgct ttggccttgt caccaaacgc tctgtcgatc acaaaatagt
    15601 catttatgtc gtatttgtga tatgagtcgg atttgaagat aggggtcaga taaatgattt
    15661 tggcaccaag accccttatg taatcaagct tttttataac cccctcgata tctcctccgt
    15721 aatacgtatt ccttcttagc tctcctttga agccaccgtg tgactcaatt gtcttatcgg
    15781 gcagaacgtt ccaagacctg gttccttcag gattgttcga agggtctccg tcaaagaacc
    15841 tatcgacgaa gatctgatag attatctctc cttcagccca tgctggaaga ccaaatatgt
    15901 cgtcgtcgtt tatgtatggg tagtagaaga agctgcctga ttcgaaatta tcaagcagac
    15961 cgtccgaggt aagaaaaata tgatccacaa cgtcgaacag ctcaaaatag tacttaaaac
    16021 gtttctcttc taccaccaat gaagcttcgt acatatcgca aatcccatcg gttagaattt
    16081 tcttcatctc ggttgtactg attctttcag aatgatcata aagatttttg tagtgaacaa
    16141 ggattctctt cacatcatct tttcttgttc taaccctcac agcaagagta tcattggctg
    16201 ttgcgtaagc gtagttgtgt tcggatatat ggtatatcga tgatctatcc attgctcaca
    16261 ctccttgtcg acaattgaac tctcatgcct ttacgctccc ttcagtcaat ccacctatga
    16321 agtatttttg aagagaatag aatagcacta gcataggaat actacccaaa acagccccag
    16381 cgcaatagac gctccagttg atcccaaatt gaccttctat catgctttga agtccgactg
    16441 caagaggcat cgcctcgagc ttctgagtca tcactgtgct cgcgattgcg tactcgttgt
    16501 acgcgattac gaaactctgg atagcaacaa ctgcaatcat gggtttagca agcggcaggg
    16561 ttatctgagt aaggattctg aatctattcg ccccgtctat cattgctgcc tcatccagcg
    16621 aataaggtat cgtatcgaag taaccttttg cgataagaac aagaccggcg gaagcaaatg
    16681 ccgaatccac aatgatcagg ccttctattt tgttcaacag tcctaaagca aggaatatcc
    16741 tgaatatcga taccatcgag agagtaagag gaaagacctg tatcaaaagc acaaaattga
    16801 agagctttgt tctcccctga aacctgagcc tcgaaaaagc gcaagcggaa agcgaaacca
    16861 cgataaccga gagaatcata gtgaccgtcg ccagcaacag agagtttatt agccagtttg
    16921 aaaatctggt ttccgtgaag agcctactgt agttgtcgag agtaagttct ttcacaaaga
    16981 atacacttgt atcaatcacg ttcttgccca aggagatcaa cacaacgaag aagaaaggaa
    17041 ttatagtcac aactgccaaa atcagaagga taaaatgaac caggattcta ccgactctct
    17101 tcttcacatt gtacatgtat ctcaccactc ctcaaattga gttttctttg tttgtttcca
    17161 taagacacgg tcacaattaa taggacttcc tatttgcgat tctcaaagat acaactgcaa
    17221 atacggcgac aaaacagaaa gctatcaccg agtaagctgc cgccagggaa tatcttttcg
    17281 tattgaaagc agtattgaat acatatgtga ttaacagatc ggtggcacca ggagagccaa
    17341 gccggcttcc aggaggtccc ccctgggtta gcaggtagac tccgaattga ttgaactgca
    17401 tgataaatga cataacgagc atgggaaata tcgctcttat aataagggga attgtaatct
    17461 tgaagaaaat gtacagtctt cctgcgcctg caattctcgc tacttcaaag tagtccttgg
    17521 ggattgattt gagtatgctt gaagagataa ccatgaaata cgggaatgaa aaccaactca
    17581 tcacaattat actgcttgct ctggcagcca aacagtgcga caaccaggga actgagttga
    17641 ttccgaacaa actcagtatc tgattaacga gtccctgatc ttcaagcatc cctctccaca
    17701 tgagaagggt gattacgctg ggaaccaccc aaggtagaat gaagatcaat ttgtacactt
    17761 tagctactct aataccaacc ttgtccagtg taacggcaag tgcagtgccc acaataaatg
    17821 aaatagtcac gaccatgaaa gcaaaagtta ttgtccagac aaacatcgat gcaagaccac
    17881 cgatgccgct cgtgaatatc tcgcggtagt tcgttaatcc tacaattccg taatccctta
    17941 gatttacgcc actataatta gtcaccgaaa gatagatgtt gtgaaatatc ggaaaaacat
    18001 agaacactag aaggagaaga agcaggggta agagcattgc ccaagcgatc atagtgttct
    18061 tggtgcctag tttctccgtt ctgtcaagag ggcttatctg cgatgtattc ctcttcgtcg
    18121 cttttctaag aagaactaca gctgcgatca atccggctag aaatgctgca aagatcaaaa
    18181 catgagaagc ctgaatatca atcttctcgg gtatttcgtt gatgagtgca acgtcggctc
    18241 ttatcctctc tgccaattct agcaacttct cctgagcgtc cacttcgttg cctgcagtat
    18301 caccgtagaa tatcgccctt agggccgtcg aagtgtagtt gtaccaaatt ggtccctcag
    18361 gaacattggg gaatggttcg cagttgaacc ctatgttgct cagagaggac agaaccgtat
    18421 cctcctcgat atacttgctg tttattacag atatattcgc ggggtttctc tctccagttt
    18481 ttctgtctcc tctattaccg gcctcaataa gcctctgctg attctcgtct ttcaagataa
    18541 actccaagaa gctctttgtc gcatctagat cattcgtgaa tctattgatt acgaacccct
    18601 gaacggttgc aagaggttta gagaccttcc cattcttccc caccggaagg ggagaaatac
    18661 catactctat tcccatagat ttgtatatcg aagcattcca caaaccgtac agtatcatgg
    18721 ctactctacc gtttgcaaat tcggcggaaa tatgactttc ggtggcattt ggattagtta
    18781 gagtgagtcc cttggactgg agttccttta ttttctcaac ataagcaagc atcccctcgt
    18841 tgtccagtcc gacatcataa ggattacagg atccgtctgg aagaactccg aagtagtatc
    18901 ctccgtactc cttaataatc ggatagttga accacatatc cttaggattt ataagagttc
    18961 cgtactgaac aatctttccg ctgctgtctc taaccgtaag ctcttctgct actagaaaga
    19021 aatcttccca ggtcttagga atatcttctt cgcgtattaa ctgtttattg tagacaagtc
    19081 ctgatgtatc gactgagtaa cccacgccgt agtattcgcc gtcatattta aaggcttcaa
    19141 aagcaacact aacaaatctc tctcttagat cggagtcgat ctcaaccggg aggataaacc
    19201 cggatttcac catgtttcca atatcgggtg cctgcatgag aacgatgtcc ggtctgtcat
    19261 aagcctcagc atagtttgct aggtctcttg gtgcgtcgaa tatcgatcgc ctggatacta
    19321 cttcgactgg tattcccgtc tctagtgtga attcacttgc aatgtctgcc atcacctggt
    19381 tttccgagtc aatatggctg aaccaaatcc gtacagcaaa atcagattcg cttgacaaaa
    19441 gcgatattcc ggtcaaaatg aatgtgaaga ctactaccca agtcttcttc aacagaaaac
    19501 cgcctcctat gttgcgcaaa gagtgcttac agttcccaaa aatctcttgc cgattgctct
    19561 gcctcttcag cagtctcaac cggcaatgtg attgagaaat ttccttttct caattcttca
    19621 atgtttccct ctatctcatc aaatctgtca cagtcaatgg aaagacagac tccgttagac
    19681 gaatcgatca ggtatgtgat gttttctatt ggtctcggtt gatttggatc agttgcaatc
    19741 cagatgcttg cgactttatc aagcgaacag atttccttaa ccaggtaaag cgctcttgag
    19801 tgacagtgca tatgacctat tccgaaatgg tcaattacac gctgagtata aggcgcacag
    19861 aatttcctgt acagtttcgg cgaaatcatg caggctgtgt cttcagacat gtagatacag
    19921 tccttaatgt gccacattcc gtatttcttg ccagcaagat acctgtgagc aagagaataa
    19981 acatcttctg cgaatgctat tagtgcgtct gcgcaatagt cgagaagact atgaagctct
    20041 gcggggaatt catagaaatc agtgtaaatt gcctctcctc gaagagacgc agcaagatcc
    20101 agcggggaga agaacccgcg aagaaagggg attccggcag gggaagatat tttcaagatt
    20161 tcctcacaga tgttcaggaa gttcttatac cagtaagccg ttcctagctt cggaagactt
    20221 ttgaagtctt ctatttctcg tagaacaggt ttgaaccagc ttgtatccct gtgaaactta
    20281 atatcaccac atacaaaagc tgaataatct gcaattccga gtacgggtga tacgcttggc
    20341 accatgttat cgtctacgtt tctcctttcc tcaaaagact ctctgtagag ttcacagagg
    20401 tccttagcat actgttcgat atcattttcg tagttgtatt tggtcagatc aaaccgggag
    20461 taaagtgcga catctgattt tggaagcgct ctaaaagtta cattccctac cttgtgattg
    20521 agtgcgtctc tgtagacttt gtccatcatc ccaacggttt tgtctagatc cggcttgtag
    20581 tatgaaagct ctctgaacat cctagaaccc ccctatatag tgatgttttg ctgcggacta
    20641 ttaaacaacg tcgactactc atcgatcctt aacggcaaat ccttcggaag acttatggat
    20701 ttgaagaaat cgtcgatact tttgtgataa tcgtctatct gaatttcttt attgatgtac
    20761 cccccgcttc tcaaagtgtg aaggagtatt atatcgagaa gtatttgatc tatcgccaat
    20821 tgcattatca tctggttcct ggtcgttatt cctatttcgt tgatttcttc gttgccttct
    20881 cccataattg cgagttctct gtcaaaaacg gctactattc tcctgcgagg gaagttctct
    20941 ttggcatctt tcattccgta tgtgtactga ataccaaaat caaatggaag cttacagaag
    21001 gagaatatgt agattttcac accgctatca tcagcttttt tcaagtcatc ttcaaagagc
    21061 cttgcttcag agtcccagat tgaaagataa atttctctct ttgcttcgat tattgctttc
    21121 ctgatgaggc taatcacttc tgttcgaccc gagatgttga gaacgtattc gttgtcaaca
    21181 acctctcggg agattttctc tagagtctct tccagctctt ctagcctcct ttcataatca
    21241 tttctgatgt ttgaaaccag tctcttgtaa gcaactgccg agtacagtct gggattgcct
    21301 tcaattacct ctatataccc tttccttgca agccgcctaa gtatgtcgta gaccattgtt
    21361 ctggttattc cggcttcgtt tgcaacctgg tagcctgtca aaggactttt tttgtgcagc
    21421 gcgatataac acttagcttc atttgtcgag aatcccaatt tgcacagagt cgtataatca
    21481 ctcatactcg atcaaccctt tcttgatatt tccgagagaa tattgtatag tagtaatggt
    21541 tattactact cttattatat attatagcat tgtgtatgtg tatgtcaaaa tatctcttga
    21601 gtagatttga ttatgatgta ttacgatatc tatctgttct aaatggcgat taagaacaga
    21661 tagatgtgga aagggatggt tcgactcagt tatctcggta cgaacatcct gtgcgaggac
    21721 tgtcaatggt tatgggattc atttctacag cagctgcgtt atgaggagcg tttcgacaat
    21781 aggttgagat gcccttttcc cattacccta tgaagcaaag gaatcacgaa cttttcacgt
    21841 ccgtgaaaga cgagaatcaa tttgtgttat cagtataatg aatacatgac acttgaagaa
    21901 aagtatgaaa gtgcaagagt acgggttttt gaacagcagt aagaagatat ctcagattgg
    21961 aaacggctcg cttaatcttc acgccggatg ttatgagact tgcgagtctg tgatctttgt
    22021 gaaattgata attctgatcg gtgttttgca ggagagatac tgtgaaactt attactaaga
    22081 agatgttttt agagtacgat tactgccctg tcaggggatg gatcgagagg aatgagcccc
    22141 gggaattcag gccgaccatt gcagaagatt tcaggatgaa agaaggcctc gatgtcggca
    22201 gaagggctag aagtctcttt cccgaaggta ttctgataga ggaaatggat ccatttgagg
    22261 cctccttcat aaccgaacgg attatcggcg atagggaatc cgtaactctt tttgagcctg
    22321 cctttatcag cggcgatttc gtctcaaggg ccgatgttgt gataaaagag ggcgagtcac
    22381 tggatgtctt cgaggtcaag tccagccttg ccggttcatc gaacacaaag gactacataa
    22441 gggatctttc ttacacggta tctgtcatag aaagcttggg tcacagggtc aatagagcct
    22501 gcttgataat ggtctcaagg atgtatcgca agggcgacga ggaccggaag ctctttgaga
    22561 tcatagatgt tacggacgag gtctctgccc agaaaagtga gataaaggca aaactcgaac
    22621 ctgcctcagt tatcctgact tcaacattga tcccttccgg ttcgctggga tacaaatgca
    22681 gggactgcga gcatcttaga agatgcttcg gcctggaaag gaagcttagc atctttgagc
    22741 ttcccagact gggtgccgac aagacagatg ctttgatcga aaggggatac ttctatctag
    22801 aagagcttcc gcgagaggca ctcggcgaca aacccctttt gcagagggtc tttcgcggag
    22861 ctaaagaagg aaggattgtg tttgacgaac cagaagaact gcgagaaaaa ctgaacagct
    22921 tcaagtatcc aataggttat ctcgatttcg agaccgccgc ctcagtcatc cccctgtacg
    22981 atggattggc tccctacgag ggtattcctt atcagtattc actgcacatt cgaagggaag
    23041 tctcggacgt cctggaacac agggagtttc tcttcgacaa tccttcgagg gatgagagtc
    23101 ttctgctagc cgaaaggctg gttgaagacc tcaaggactg cagaacactt atggcacacc
    23161 atgcttccgt cgagaggaat attctgagat ggctgtctaa cagatacaaa gattttccgg
    23221 agctttcaga acagctcata tccttctcgg agatgtttgt agattcagaa ctccttgtga
    23281 aaaaccacat atatcatccc gacttcggcg gaagcttctc gatcaaaagg cttcttcctg
    23341 cgctagtgaa aggtgttggt tatgagggac tgtttataca caacggtgac gatgccagat
    23401 atgttttcgt gaacatggct cttggaaatt acagagagtc agagatagaa aaaataagaa
    23461 gagatatgct tgagtactgc aggatggata cactggcgat ggtggagatt cacgggtttc
    23521 tctgcgatct ttcatcggag taaggatagt acaaatggat aaaagagtaa tcggcagacg
    23581 acttaatcga attcttttca aagtagaata gtttggtcat caaattaggt tattatgaat
    23641 catgcacact ttaggcatcg agaggatgtg acgacataga agtcttcaag ataaagaatc
    23701 atgaaacatc gcttccgcta attcagggag gaatggccgt aggaatatct ctggacaacc
    23761 tggccgcttc cgttgctaat gaaggtggaa taggggtcat tggaacggca ggtataggta
    23821 tgacggttga aggttacaga agcaacttca ggcaggcaag catcgatggc ctcaggaata
    23881 ccataagaag ggcaagggag aagacaaaag gggttttggg agtcaacata atggtggccc
    23941 tcacaaacta cgcagagatg gtggcaacgg ccgtgaagga gaagatagac gtgatcgtct
    24001 caggcgcagg actgcctcta gatcttccct cttatctcga agaaggatcg gagacggcca
    24061 tcattcctgt ggtctcatct ctgaaatccg cgaccgtaat attcaagaga tggtttggca
    24121 ggtacagata cgtccccgac ggcttcgttg tggagggacc taaggcgggc ggccacctcg
    24181 gatacagagt cgaggagatt ttttccgaag agagttcgct ggagaagacg ttacctgaaa
    24241 ttagaaagtt tgttgataga gtaaaacttg atactgggaa gaatatacct gttatagctg
    24301 ccgggggcat atttgacaga gatgatgtcg agagagtctt caagctggga gcttctgctg
    24361 ttcaagtggg aacggccttt gttgccacgg aagagtgcga tgcagactac aggttcaagc
    24421 aggcatttgt cgatgcgcgc agcgaagatg tcacgataat caagagtccc gtgggaatgc
    24481 caggaagagc aatcaggaac aagttcatag aggatgtcga aaatggtaaa agaaagccct
    24541 tcaagtgcgc ctatcaatgc ataaagacat gtaattataa agaagcgcct tactgcattg
    24601 cggaggcact attgaatgcc tgcaagggag atctggaaaa cgggttcgcc ttctgcggaa
    24661 gcgaggtcca cagaataagc ggcgtcacta ccgtgaaaaa ggtaatagat agactctttg
    24721 gagaaaggcg ttgaagaaat ctttcggatt tacgtttttt atgccttgct ctttgaatat
    24781 tgaaaactgt acgggaggtt gaagatggaa agtctggaga agataaggaa ggcgataatc
    24841 gagacaatgc cggcagaggc aaaggtagcc actgaagagg ctctcgcgga aggactcagc
    24901 gcgaaggaaa tccttgatgg tgcgcttatc ccgggtatgg acgaagtcgg gaagctcttc
    24961 agggagggcg agtatttcgt tcctgaggtc cttgttgccg caaaagcgat gaaccagtgt
    25021 atggacctta tagagccgct tcttgtaagc ggaggggtca agaagatcgg gaaggtagtc
    25081 gccggtacca tagaaggaga tctccatgat attggaaaga acctcgtctg catgatgctc
    25141 aagggagcgg gcttcgagat aatcgatcta ggtgtcgatg tgaagcccga agagttcgtt
    25201 gaggcagcca agggtgcgaa gcccgatatt cttgtgattt cagccctgtt gacaaccacc
    25261 atgctgaata tgcccagagt gattgaagaa cttaagaagg caggaattcg tgacgatgtg
    25321 aaggtcttga taggcggggc ccctgtcagc cagacctatg ccgaagagat cggggcagac
    25381 ggctattctc cagacagctc ggcagcagtc gaattggcaa gaagactcgt atcttgattg
    25441 atccactggt tatttgtgtc gatggggatc ggcattaacc tctggagaag acgaaaaaca
    25501 tgaatgattt cccgctccat ctctttcaag cttcatagcc tgttttgacg gcagcgagac
    25561 gaattgttat aatatttagg tattcattcg tttatttgtt ttgtatggtt tttatttgat
    25621 ggcggataga ccgtctcgtt acggatggag ggttaagtat gcttatcgcc ctgataattc
    25681 tgtcgatagt tacactgatc tcctttctct tcgcgattgc atcagttaga agattatccc
    25741 gtagtatcaa gaagaaccag gagctacaac tcagccttaa ggatctcgaa agggatgtct
    25801 cggatagaga tgagaagctc aagtggctga cttctgccaa ggaggaactt gaatcaactt
    25861 tcaaagcgat ttcctcggac gttttgaatt ccagtactga aaacttcatt cgacaggcaa
    25921 acgacaagtt ctctggccta ctgaaactcc agaaagaaga ctggggaagc cagaaaaagg
    25981 atttcgagag ccttgtcagc ccggtaagag acaatcttga aaagctcgag aaaaatgtta
    26041 aagagcttga atcgaaaagg gaaggagctt ataaagctct tgaagagcag ctgaagatgc
    26101 tttcagaagc caacaccgac ctcagacagg gcgtaacgga tctcaagagc gccttgagaa
    26161 acagatcgac acgcggccgt tggggcgaga tagagcttcg acgcattgtt gagctcagcg
    26221 ggatgaccga gcatgttgac ttcgtggaac aggagtctat cgataacggt agaccggaca
    26281 tgatagtgaa gctgcccaat aacgccttca ttcccgtcga tgccaaagtg ccgctggaca
    26341 gttatttgac agcgctggaa gaagaagacg aatctcttag aaagcagctc atgggtaact
    26401 acgcgaagaa tgtccgcgat accgttaaca ggcttgccgg caagaaatac tctgaggcct
    26461 tcagtagaac ggccgatttt gttgtcatgt tcgttcctct ggagtcggca atcaacgccg
    26521 cttttctcgc agatcctgaa cttttcgaat atgccgtttc aagaaagatc ctaatcgcct
    26581 ctcctgtgaa tctcgttgcg ttgctaaagt ccgtcgcgta tggatggcag cagtatagtc
    26641 ttacagaaaa tgccgagcgt ctcgttcaag cctctaagga gctctatgaa agatttcggg
    26701 tctttttttc ccattacagc agattacagg gccatcttaa gaacgccgta agggcctaca
    26761 atgatggggt gggttctttt gaaagcaggc tgctcccaaa agtcagggag atagaagctc
    26821 ttgccgaggt aaacgaaaaa ctggaagaga tcgaggagat cttggatgag cctaaatcgt
    26881 cggatcttgt cgaccgcaac tgaattgctc atccgtcagc gcttcattga ggaatatgct
    26941 tgaacacgcg aagttctcgt ggtaacggtg ttttcttgac gacccggtcc ggccgcttct
    27001 tggcatagtc ttagtgagct cctcccgcat ctaattgtgc tttttactta ataatcaaat
    27061 gcaataccat atgagatcaa acgaaacatc cttcggtggt ttcaagatct tttatgcgat
    27121 aaaatgcgaa agggagcttt gaggtttcca agatcatatg aagaaggaga aatagatgaa
    27181 tgagattctc tggttcttaa tgctggtagt gaactttgct ttgattacca tagcatacag
    27241 atggtgggga aagacgggcc tttacgtatg ggtagccatt gccgcgataa tagctaacgt
    27301 gcaggttatc aagacagtct ctctcttctg gatggccgcc acgctcggaa atatcgtata
    27361 tgcgacctct ttcctcgcta cagacatcct atcggaaaac cacggaaaga aagaagccag
    27421 gaaggccgtc tacgtgggat tcttctctct aatatccgtt actgtgataa tgcaaattgc
    27481 gctcgccttc gagccccacc cttcagactt ttcgcaagaa cacctttctg tggtatttgg
    27541 tctccttccc cgcatcgctc ttgcgagcct tgccgcatac tggtgttctc agctgcacga
    27601 cgtgtgggca tacgacttct ggaagaagag gttccccgag aaacggcaca tctggataag
    27661 gaacaatgcg agtacaatga tctcgcaatt gatagacacg gccgtcttca ctgtcattgc
    27721 ctttgcggga gtctttccgg caggtgtctg gtggcagata ttctggtcca cttatattct
    27781 gaaatgggtc gttgccgttc tagacacacc gttcatctat ctcgccagaa agatgaaaga
    27841 cgacgggaag atcagagatc ttgttcctta acccttatca taggctgaaa atactaaagg
    27901 cgtgaattgt tgaagcttct gtttttggaa aaccgccaat ctccggctgg tataattggg
    27961 ccaaatatcc tctgttcagt ggtttggtac ttcagctttc acgtatgttg ccatgtcagg
    28021 aggtgaacag tgaaagaaag agaagagaaa ctagataaat acgtggaaaa gagagatttc
    28081 agcaagacat cggaaccgga cgggagagag gccactgttg aatggtcaaa agagaggccg
    28141 gtattcgtca ttcagaagca cgctgcttcg aggcttcact acgatttcag aatagaagtg
    28201 gacggagttc tcaagtcatg ggccgttccc aaagggccct caacagatcc atcggaaaag
    28261 aggctggccg ttccaaccga agaccatccc cttgaatacg gcgacttcga gggaaatatc
    28321 cccgaaggcg agtatggagg cggtacggtc cttctatggg accggggaag ctacagaaat
    28381 ctgaaggaag accctgaatc gaatcctctg tcgaagcaga tagaagacgg gcatatcacg
    28441 atttggctcg aaggccataa actaagaggc gggtacgctc tcataagaac cggtaaaggc
    28501 agcaaagcga gatggcttct cgtgaagatg aatgatgagg aggccgatgc gcggagaaat
    28561 ccgacagcca ccgaaccgga ttcggtaaag accggtagaa ctctcgaaga gatccgtgat
    28621 tccggagagt aatgctgatt tgaaagaccc ttgttatctg gagattgatc acacagtata
    28681 tgcctgccct atcttgaaga cccgaaaagt cttgaaatct ctcgaaggcc tttcaaatcg
    28741 aaaaaaaccg gctttctgga gttgcctcag tcatttgagc aggggtattc gtcgataatc
    28801 tctggattct taaggccctc cctaactttt acaaggatct cggctgtcag tatcttcagt
    28861 ttcaacagat gaatggaaac ggtagaggca accccgatga ggacaagtgc atcggcagta
    28921 ttggatatca taatcatcgt caaggctaga gtagaccaaa gcaacagaaa ggcaaggttc
    28981 atcgctctcc tccttacggc gtgaaaatag agatagctga agatagatgc gacgaagagc
    29041 ctgtgacaga tcggccatct gtaaagcttt tcagagctcc tcgattaaca aaatgccgtc
    29101 aacaacaaaa agtgagttgt tggcagcaaa ggaagagtta ttttcgttat ccaaagaaat
    29161 agaaacagcg cagtgagttt gactttcttc ttcggatctt tttccatgtt tttctcctgc
    29221 cttgattgta ccgaagagcg tacaacgttg ggcaggcttt aaattaggtt ctcggcaatc
    29281 tggcggttaa gtttatggaa gtacaatcgg ctttgaatca gagaaagcgg tgtaaagaga
    29341 aaaaaagagt tcgatcggag aatcaagacc gttagtcctt cctacttttg atgattctga
    29401 catgacatta tgctgacttc ttctaaggga agaagagatg gtagttcaac ttctggatct
    29461 ccccgcttca gagttttagt taggtttaat aacttctata ttgtgatgag gattctgata
    29521 tcattaagat gaggtgattg aatgcacgca gttctctcaa agaaaagaat cgctcccgat
    29581 acgtatgata tctggctttc tcatccagat atctacagat acgcaaaacc aggtcagttc
    29641 ctgataataa ggatagacga gtttggtgag aggattcctc tcacgatagc gtcggcagaa
    29701 aaagacaggg taagggtaat agtgaaggcc gttggaaaga gcacctataa actctgtgca
    29761 atgcacgaag gagaatccgt agccgatata gtcggccccc ttggaaatcc cagcgagatc
    29821 gagaaacttg gaacggtctg tgttgttggt ggaggcgtgg gaatagctcc tttgtttccc
    29881 gttgcaaggg ctctcaaatc tgccggaaac gaggtaatcg gtatactggg tgcggcgaac
    29941 aaggatcatc tgattatggt cgaagagttt aaaccctttc ttgacaggct ttacctctct
    30001 acagatgacg gctctgccgg agttaaggga accgttacga acccgctcaa agaggtaatc
    30061 gcagattata cgccagcaac gatatgggca atcggtccga tggttatgat gaagttcgtc
    30121 tctcttctcg cttcccagaa tgacatacct tgctgggtgt ctctcaatcc gataatggtt
    30181 gatggcacgg gaatgtgtgg cgcctgcagg gttgaggtcg gaggcgagat gaagttcgcc
    30241 tgcgttcatg gaccggagtt tgacggcaga ttggttaact gggacgagct gatcaaaagg
    30301 cagtatcagt acagagaaga ggaacggctt gctcttgaga aatacgtaca tgaattgggt
    30361 gagaaggatg gttgagaagt tcaaagtaag aatgagggaa ctcgaccctt cggagagagt
    30421 taacaacttc gaggaggtcg cacttggcta cactaaggaa gaggcgatcg ctgaggcaaa
    30481 tagatgtctc cagtgcaaac acaaaccctg tgtggccggt tgccccgttg gaatagatat
    30541 ccccggtttt atcaaggccc ttagagaggg tgacatcgag aagagcacga gaatcttgaa
    30601 agatgcaaac aaccttccgg ccatctgtgg acgagtttgc cctcaggaga agcagtgcga
    30661 aatggcctgc gtcgtgggaa agattcccgg cagcgagccc gttgcaatag ggagactgga
    30721 gagattcgtc gccgatcaga atattccact cgagaccacc gtgaagccgt ccgtaaacaa
    30781 gagagttgca gtaatcggtg caggtccggc cggattgacg gccgccgccg atctcgcgaa
    30841 agagggtttt tcggttacag tcttcgaagc cttccatacg gcgggaggtg tgcttgttta
    30901 tggtattccc gaattcagac tccctaaaga gatcgtcgca aaggaagtcg aatttgtgaa
    30961 gagcctggga gttgaattcc gtttcaacca gattgtcggc cgaacgattc cattcgaaga
    31021 gatcaagagc gaatatgatg ccgtcttcat aggtatcggc gcaggtgccc cgagattcat
    31081 ggggattccg ggatcgaatc tcaacaatat attttcctct tccgaatatc tcacgaggac
    31141 aaacctgatg aaggcctatc tctttccgat gtacgacaca ccagtaaaga taaaagacag
    31201 agtggccgtg attggagccg gaaatgtgac gatggacgct gcaagaaccg caaagaggct
    31261 cggagccaaa gaggtccatg tggtctacag gagaagcgaa gaagagatgc ccgcgagaat
    31321 agaggagtat caccatgccg tagaagaggg aatcgtgttc cactggctaa cccttcccgt
    31381 tgagtacatt ggagatataa acaacaacgt caccgggatg aagtgcgtaa agatgactct
    31441 gggagaaccg gacgattcgg ggagaagaag accgatacag gttgaaggat cggaattcat
    31501 catggaagtt gacatggtca tcgaagcaat cggtcagact ccaaatgcca ttttgaaggc
    31561 cggtttctca actgtaagac tgaacaaatg gggcaacatc gatgccgatg aagccagcgg
    31621 cagggttgac gaaggcatct ttgccggcgg agacatcgtt actggttctg caacggtaat
    31681 agaagcgatg ggagccggca aacgctcggc gagggcgata aagtcatact tattgcgtaa
    31741 gagataggct tcagcactgc aataatgtat aaatgtatat ttgtggagag ttaatctaga
    31801 gcatttccta ataagaaaag tcgacggccc ttctgggttt gtcccaaaca attgctcctc
    31861 attcttctga gaaagtctcg cctgcttaga ggtgacttac ttttttctgg acagatcaga
    31921 tggcaacctc tcgaagaaaa tcgccgtttc attagttgag aaggcattca ggacgctaaa
    31981 gggagtggat ctcaagatac ggccaataca ccactggctt gagaagagag ttagggccca
    32041 catatttctc tgcatgctag cgtattatgt tgaatggcat ttgagggagt catggaaggg
    32101 catgatcttc accgactaat atgccgatag agaacattta ctacaggcta ctagatccaa
    32161 aagtgcactt gagaagacga gaatgaagaa gctgaatgaa gttgtctgtc taactggagt
    32221 tcgatccctt tcccggtttt gcacaagaga ttccggaaaa tgggaggctg gcctcagctg
    32281 gccagaaata caagagggca cgatttgcgc cctctttttt tgtctcgatc attttgaatt
    32341 cagaaagtgg ttaactgtcg gtttttccgg aaacaacaaa gaattctaaa tcggtagcgt
    32401 gacgatcgtt gctacgaact caccttcatt aactattgaa atagctccac cgtgcaattc
    32461 gataatcctt ctcgttatgc tcaggccaag tccattttct cctccaaccc ccttgttgaa
    32521 gggtttgaag agctcttcca gtttggtttc ggcaactttc tctccatcgt ttgcgaatcg
    32581 tatcaaaaga ctctcttctc tctcgtttac tgccatagtt acctctattc tgctcttcgc
    32641 ataccggatc tggtttgaaa gtatgttttc aatggctatc gccagctgtt cttcattgac
    32701 ctcggtcttg aagtctttaa gattcagatt ccagtctatg tcgtctctct ggaaagaaaa
    32761 tcgcccgact gtgtcctcaa ggagttctga gagattgact tcctcttttt cgaggtcgtg
    32821 acgcgacagg tattcgagtc tggttatgaa gagaagatcc tttaccctca gatccattct
    32881 ctgaatctct ttatctatga cgtctatagc gctatccagg tcacccgcgg gatatatgcc
    32941 gtccttgatt gcctgtgcgt aacttcggat aaccatgaca ggtgtcttta gttcatggga
    33001 gataaactga agcatcgact gctgttctct gtcctgctcg ctgaggctct ttctcatgtt
    33061 ctcgattgaa tcggctagct ctccgatctc gtcgcttcta ttcaaagaaa tcggcctgtc
    33121 ccagtttctc tttgagatca ttctcacgtc ctctgaaagc tttttgagag gcttagtcag
    33181 gtatctagaa aagactatta tgaaaactag actgaccact gtcattatag ccattacgag
    33241 cagcagcctg gagaacaacg tcgatgaaag agtgttcatg tacgtatccc acatatagga
    33301 aatcatcgtg acgtctcttc cagagatctc ttcattgcga atcacataga aaagactccg
    33361 gccggaaagt agtgtttcgt acctctcaga agatgacgtt tgcttttctg cgttttctct
    33421 aatggattga atggcaggaa gtatttcggg gataatcgtt ccgctaatta agatgtccct
    33481 ggaagtggaa tccggagacg gggaaatctt gtctgagcta tcctgggttc caaatctgtt
    33541 caggtaataa tctattaggt caaactccct gtcgaagacg attatgtgtc caacgtctct
    33601 gacatttcta agactgttga ggtcgatatc ttcgaaaggg atgaaccctc cctgcaattc
    33661 gattaccctt acttcctgag cgtactctat cgttctgtac gcttcggttg tgaagaagtc
    33721 tcttatagac catctgaaga ctataccgag aagaaggaag aggattgaaa gaacggctgc
    33781 gaatagtaac cagagcttgt cggacaggct ccacgatctc atatgttcac actcttatag
    33841 ccgaaaccgt agacggtctc gatcttgaaa tgtggcatct tctttctaag tctccgtaca
    33901 agatcgtcaa ctgccctgtc gcttccgtag tggtcgtaac cccagactct atcgagtatc
    33961 tggtctcttg aaaaaggttt gcctttgcca ctgataaata gcaggagaag gtcgaactcc
    34021 atagaagtga gctcaatcag ttttgactga ttctcatcga cttcttcggt aaccgttctt
    34081 cgcgcttcgt cgatcttgta tggtggaacg gatatgattc caccggaatg gtggacgcgt
    34141 tcaagaatct tcttgacccg aatgacgagt tcccttggaa gaaagggttt tggcaagtaa
    34201 tcgtcgcttc ccatctccag tccgatcact ctatcgatgt ccttgtccct tgctgaaatg
    34261 aagatcaccg gtatcatcga atccctcttt cttatttctc taaggagctc gaagccatcg
    34321 attcctggca gcattatgtc tagaatccag atgtcgggag agtcatcgat ttcatcaaat
    34381 gcatcgctgc cgttcttgaa cgactttact tcccatccct ccttttcgag ataggaagtc
    34441 agcacgctgt tcagattttt ctcgtcttca acaagattta ttcttgttcc catgaatcac
    34501 acctctgtga cttccgcctc attcaataat aatcgattac tctgtgaatt actaaccaat
    34561 aaaccggact gcaaggatat gtctcatttt caatattgca gtccggcctt tccaaagaac
    34621 taccttttct gagtaaccaa caaaacactg tcacatgctg cacagtcttc gccactatct
    34681 attgccgttc tcagattatc acgctccttt acacgtaccg aatggggcgg ttccagtaat
    34741 ctatgtcaga ctgtcatcgt agctctgcta ttgttcacgc tgttctttgt taaagagctt
    34801 tatagaaact taaccgggct caacgctctt aactgccaca actattgtct caactccatt
    34861 gctcaccctt gtaacctgaa tcgagaactc ggggggttcg tagataactt ccgtggcaga
    34921 cggggatgtg ttgtgatact tgtgaaccgg tgtcatatct agcagcatta cttccgtaat
    34981 cgattcatct tcccaagaac gcagtgtttc ctccagatcg tcagggtcga tcacgcggtt
    35041 atcggttacg agatgcgaat cagttccttc gatttcgctc accacaactc tcttcagaga
    35101 atgcaggccc aggtccttca gcttctggat aaggtcgtcc aaagtcattt ccgttttatc
    35161 tgaatatcgc ttctcatggc ttgtgactaa catttcttca actccttaga agtctctgag
    35221 atctagtttc agtaatatta tagaaagtaa tatgcgagag aattcggcaa aagtgtggga
    35281 tataatggga ttcataggac cgggattcca taggtcttgc aattggaagg ccattaagat
    35341 gctcttattc agctatcgag ctacctacgg aggtgtctca tgaaagggat tattaccgtt
    35401 ctattcatac tgtgcgcagt tattctggct tcagagcctc taagcatagg ctttatctat
    35461 gtcggttcgg ccgatgatgg cgtctggaca gagaaacacg atgaaggcag gcggtatctc
    35521 gaaaagacgt ttggaagtca aatcgagact tcatttatag aaaacgtgac tgaaggagaa
    35581 caggacctgg aggttcttag aagttatgcc gaacgtaacg tcaaactcct tttcagtaca
    35641 agcttcggct ttatggacga tgtgatggag atagcgaaga actatcccga tacgatcttc
    35701 atgcattgtt cgggatataa gaccgctgaa aatgtcggaa cctacttcgg aaggatatat
    35761 gagccggcct acttgaccgg tctcatcgct ggggagatga caaaaagcaa cataatcggc
    35821 tatgtagcga cattcaagat tccggaagtt atcaggggga taaatgcttt tgcgattgga
    35881 atcgcaaagg tcaatcccga tgcgcgcgtg cacgttatct ggaccgagac ctggttcgat
    35941 ccctcttccg aagaggaggc ggccgacgcc ctcctggatc ttggagccga tgtaatcgct
    36001 cagagccagg attcgccagc tgcagttcag gcggccggac aacgggacgt ttacgctatt
    36061 ggttacaaca ccgatatgag tgccttcagt ccagacactt ttctgacttc tcccgtatgg
    36121 aactggggag tcttttacga gagagtcgta aaagaagtaa tggagggaaa gtggaccagc
    36181 tatcaatact ggggcggaat agaggacagt gtagtcgaca tagtgatgag taatctagtt
    36241 cccgccgatc ttcagaaact tgtcaatgaa gaaagaagcc ggataattga ggacgattat
    36301 catccctttg aagggccttt gcaagatcaa gacggaaaac taagatattc agagggtgaa
    36361 aaaccaaccg acgaagagct tttgaccatg gactggtttg tgagtaacgt catcggcagc
    36421 ccgaagtgac tcttatcttc ttagtttgat agagcaggcc aggcgaaacc agacttgcca
    36481 gcgaaagcag actggcttct gaattcctcc cgcggaagcg gcatcacttc ttctcttggt
    36541 cttctcggag gagaaccgtt gacggacaac gttgttttca gcagcgctca gcggttgtta
    36601 ggaggagatg ctgaaacaag ttcatgacgg gatgtgacgt ttttgttcag cctctggctt
    36661 gcaacttgga actgagaact tgcaactgct cttctaagga cgggtccatg atccgggact
    36721 ctaaccaaga acgaagaact gatcttcgct ctggaacgaa gaaaaggctt tttattcggt
    36781 gaacggagaa ccattcaacg gtgatcccgt ttcacttgct cttggcactt accaacccgc
    36841 aaccaccaac cccgaacctc gtcatttatt cttgcaactt ggaactcaca acttgcaact
    36901 gctctcgaag gacggctctt aatgctcttg atcttaaatc cccgcttgag cgggggggac
    36961 cgcttgcggt ggggagtgtg gtctttgaac aacttttgga ataggttggc cttttcgcaa
    37021 ttgaactaca aattcactct tcaagctgtt tggtctgtga ttcataggta tgctcataga
    37081 agcaaccagg aatgaacatc gaacaaccgc tctaaaagaa ctatttgaga gcgtaggctt
    37141 ttttcgcata caaagaccaa caccctccgc cactacgtag ccctcctccc tcaagggagg
    37201 attaaaatcc ttattcagaa gcacaaacag tttctaaagg atcgtccctt gatccgtggc
    37261 atggactgtt ttttcactgc gtgaagcatt ttctttcaaa caaacagcag tcgtttcgac
    37321 ctacatagtg agagctggcc tgcgaaagca gactggcttc tgaattcctc ccgcggaagc
    37381 ggcatcactt cccgacgaag gcggcctcgc ttcctgccgg aggcagcatc acttctactc
    37441 ttgctcttcc tacccgcaac cacctacccc gaacgcgtca ttaacgcacc gcgaagaaag
    37501 agcctatttg tgctttctta aaggtatccg tatttcaaat acgcttcctc cgccagggcg
    37561 gttctcgacc gttatttcac cctccagcga gccaaccatc tctttcgcta tggatagacc
    37621 catgccgctt cccgaactat tgtgagattc gtctatcttg tagaatctgt caaatatcct
    37681 ttccttctcc gggtcagcta ttccggggcc cgaatcacct atctgaatga agagtgaacc
    37741 ttctctctca aaagccctaa caatgacatc gctcttctca tcggtgaact tgatcgcatt
    37801 ttcgagcacg gccgcaaccg ccactttaag cttgtctaga taagtcttga cgatgagatt
    37861 ttcatcggtc tcaacgatta ttcggacacc tttcgactcg gcgaaggctc tgtgatactc
    37921 tttcatctct tcgagaaact ctagcagttt cacatcctgc gaagacatta cagctttgct
    37981 atcgttgtgt gcctttgcct gtagcaggag ttgattgaca acattctgca tatttcgggt
    38041 tgcaccgtca atcgcatcga tcttatctct cgcttcccct tccagtggcc tgctcttgag
    38101 tagatctacg ctcagcctca taactgtaag gggcgtcttt aactcgtgag aggcgtcggc
    38161 ggtgaagcgt tgaagcctat cgtagctttc tctcagaggt gtgagaacgt atccggcaag
    38221 agccataccg actatccagg agatggcggc aacgagcact ataagtagaa taagcgaaaa
    38281 gagaagacta gtagattttt cgctcagtcc ttcggttgat cttccaacgc gaagaaagaa
    38341 gaccggctgt ccagccatgt ttctaatcgc tctggtaaga atgttgtatt cgagttccga
    38401 gtcgttgtaa tcaacgacct ttgccttcga agtcccctca actaacggca tactgctctt
    38461 tattcttcct ccgagctgaa gaacgctctc tccgtcagga gtgacgaact cgaggatctc
    38521 attctccgcg gtgaagaggt ctaaatttcc ggaatagttt ctgagcattc tctccagggc
    38581 tattaagggt gcccttccga ttctggattc gatcctgtct gctactctgt tgagggcggt
    38641 tatctcggcc cctctttcaa gcctgaggga gaggacgaaa atgaagaggc tcaaaaccga
    38701 gacgagagag accactatga gggtgaagaa tagcgtccat ctcctctttg cctttctgaa
    38761 tgacagccct tcacctgtca ctgatttcat agcccattcc cctgaccgtt tttattaact
    38821 tctttcttcg ccctccgtct actttcttgc ggatgttctt tatatggctt ctgattacgt
    38881 ccgaccacag ttcgtcgtct tcattccaga gatgttcctc gagctcgctc ttcggaacga
    38941 ccctgccact gtttagcatt agatattcaa ggatctggta ctccttcttt ctaagttcta
    39001 tcttctcgtt tcccctgctg gcagtcttga gcgacatatc aagctccaga tctcctactc
    39061 taagaaccct tgaaggagct ccgccgaact gggcccttct taaaagagac tgaatcctga
    39121 cgacaagctc ccggatatcg aatggcttcg gaagatagtc gtcggctcct aagtcgaagc
    39181 cccggacttt gtcttcgatg ctgtcgagag ctgtaagcat cagtacgggt attctgcttc
    39241 ccgaatctct gatcttttca agaacctgcc agccggtgag tttggggagc agaatgtcca
    39301 ggacaataac gtcaaatgga ccgttgagag ccatataaag tccctcttcc ccgtcgactg
    39361 caagttcggc tacaaagccc tctctttcga gagactcctt aagcaatctg cccagactac
    39421 gctcgtcttc aacaatcaga atcttcatct cttctcctcc aatatgaaat cataccagta
    39481 gaagtgtgaa atcttcgaga aagctgcgga agatcatctt ccaaaaaaac ttctgcaaca
    39541 gaggccccaa acagaagccc tgaacagaga ccccaaacag aagccctgaa caagagcatt
    39601 tcagggcagg ctcattgggg caggcttcag ggcaggctcg gtggggaagg ctcagtggaa
    39661 cgacaacgaa tgcttattcc gaaaacgtct gaatcggtca tgcttaactt gtttcagcat
    39721 ccaggcaggc ttaccgaaaa caacaaatac cgtgaaagcg tcctgtaatt cttaagaaca
    39781 caacaaaccg tcatctgaac tccattcaga atctcgatgc tctcttgttg aatgtcagta
    39841 acgagctcac ttcattgccc tttagaaaaa ggtcttcttc ttctcacaac ttgcaacttg
    39901 gaacttgcaa ccgttctttt ctgagaacgg agaactattc aacggacaac gagttctcct
    39961 gctctttcca accaccaacg cgctgccccc ggccacggtc ttcaaaagtg cagatcccgg
    40021 atcaagtccg ggatgacaga gtgagggact tccggaaacc gctgcctgga caacttcctc
    40081 agttgcattg aaaccctgaa ttgattgcca gttctcttca cgagacccca gaccctgtaa
    40141 tcgataccct gcgctccgcg tcatctgaac tccattcaga atctcgtgtt ttttgattta
    40201 accaagaaca aagacagatc ctcgctcttg aacgagcaac gggttctcct cggag
//
LOCUS       DAOQYI010000017        38761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1220_length_38761_cov_77.687516, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000017 DAOQYI010000000
VERSION     DAOQYI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 38761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38761
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_38761_cov_77.687516"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(923..2545)
                     /gene="groL"
                     /locus_tag="PKL93_06200"
     CDS             complement(923..2545)
                     /gene="groL"
                     /locus_tag="PKL93_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868962.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNQ70891.1"
                     /translation="MAKILKYSEEARRSLEKGVDAVADAVRITLGPKGRNVVLEKSWG
                     SPTITNDGVSIAKEIDLEDKFENLGAQLVKEVASKTNDIAGDGTTTATVLAQAMIKEG
                     LKNVTAGANPILMKKGIQKATEKAVSHIRSLSKKISSREDIASVAAISANDSEIGELI
                     AEAMDKVGQDGVITVEDSKTLDTYVEFVEGMQFDRGYISPYFVSDPEKMEAIIKEPLI
                     LITDKKVSAVKPLVPILEKVAQAGKPLVIIAEDIEGEALSTLVLNKLRGTLDSVAVKA
                     PGFGDRRKAMLQDIAILTGGTVISEEVGLTLENATIDDLGSAGVVKVKKDDTIIVDGK
                     GDPEKIKQRIGQIKVQIDQTTSEYEKETLQERLAKLSGGVAVIKVGAATETELKEKKH
                     RIEDALSATRAAVEEGIVAGGGVVLARAKKPVQELFDSTENPDIKIGIKVVMKALDAP
                     IRQIVENAGLDGAVVVDKILHSDDAAFGYDALNDVYTDMFKVGIIDPAKVTRSALQNA
                     ASIAGILLTTEAALTEKPEEKPQAPMPQMPEY"
     gene            complement(2559..2828)
                     /gene="groES"
                     /locus_tag="PKL93_06205"
     CDS             complement(2559..2828)
                     /gene="groES"
                     /locus_tag="PKL93_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNQ70892.1"
                     /translation="MKVVPLGTRLLIKPYEEEKKTSGGIVLPDAAKEKQMTAKVIAVG
                     EKVEDIDLKENDKVLYSKYSGTEIKIDDDDYIIIDQDDILAKIED"
     gene            complement(3050..4468)
                     /gene="pyk"
                     /locus_tag="PKL93_06210"
     CDS             complement(3050..4468)
                     /gene="pyk"
                     /locus_tag="PKL93_06210"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNQ70893.1"
                     /translation="MRKTKIVCTIGPATESLEVLKTLLKRGMDVARLNTTHGDISEHR
                     ERIRNLKEIRKSANSPLTILLDLSGPKIRTGVFKKDAVDIKRGSEIILTTEDIEGDEK
                     IVGVNYKKLPEEVQPGNLILMDDGKIKLRVESITDSEVKTKVLNGGIVTHRRGINLPG
                     IDISIPAITEKDKKFIKLGIEERVDYFALSFVRRSEDVLHAKKVIKDLGGSIPIVSKI
                     ETEQALKNIEQIAEVSDGLMVARGDLGVEIPVEEVPVAQKKIIRYGNQKRIPVITATQ
                     MLESMIENPVPTRAEATDITNAILDGSDAIMLSGETSIGKYPLEAVSVMDLTARAAEK
                     YLKQNPELLKWTREKISTDDHTDAICRAAWDISEELKVKVIVSSTFSGHTARNVSGFR
                     PRAHILAVTPNEETYYRLNLVWGARPVLMGLGSSTDDLVRVAGPLARRLKLVKSGDTI
                     ILTAGIPFGTSNSTNILKLETA"
     gene            complement(4482..5444)
                     /gene="pfkA"
                     /locus_tag="PKL93_06215"
     CDS             complement(4482..5444)
                     /gene="pfkA"
                     /locus_tag="PKL93_06215"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNQ70894.1"
                     /translation="MIKKIGVLTSGGDSPGMNAAIRAAVRTAYTDEIAVVGIRRGYSG
                     LLDEEFIDMDYSSVGGIMEKGGTVLRSSRCEEFRTEEGRAKAADLLRGNGIDALVVIG
                     GEGSLNGAKLLMDENGIPVIGLPGTIDNDIAMTDMCIGVDTCLNTCVETIQKLKDTAS
                     SHERAFVVEVMGRNSGYVALASGMAVGAEAIIVPELPVDYEAIAEKIWNERKRGKINC
                     IIVVAEGASSAYTVARHVEHRIGYETRITILGHIQRGGSPTAFDRVLASRMGYASVKA
                     LEEGKAGMMMSLNGGKIKGIPLEEVLSHKKELDMELMEMAKILS"
     gene            5593..5826
                     /locus_tag="PKL93_06220"
     CDS             5593..5826
                     /locus_tag="PKL93_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNQ70895.1"
                     /translation="MKILKSGVPEFEAKMFQEILLEEGIFSELVDSHFAYTDSIYFGS
                     GGLVDIIVPDEVFDEAVQIFEDLKKTGKEEMDE"
     gene            5819..6925
                     /gene="gcvT"
                     /locus_tag="PKL93_06225"
     CDS             5819..6925
                     /gene="gcvT"
                     /locus_tag="PKL93_06225"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNQ70896.1"
                     /translation="MSELKRTPLFDEHVRLKGKLVDFAGWEMPLQFDSIINEHNLVRK
                     VAGLFDVSHMGEIEIEGPDAIHFSDYLVTNSVSSLKNGAIVYSPMCNEKGGIVDDVLV
                     YRLSNAKTMFVVNASNKDKDFKWITSNKGSFDVRIKDASADFAQIAFQGPRAEEILSE
                     VSQVRLEKIPFYHFEYGRVNGIKALVSRTGYTGEDGFELYVDPEAVVALWRKILELGS
                     SIGVKPIGLGARDTLRFEAAYMLYGNELNDYNSPLEAGLKWTVKMEKDFIGKEVLEEQ
                     LANGTKYKLKGLELSGKSIARHGFEVFDGEKKIGWITSGIFSPTLQKSLALAYLEKEY
                     WKIGSEVQVEIRGKRSPATVVKTPFYRGSVKSKG"
     gene            6938..7309
                     /gene="gcvH"
                     /locus_tag="PKL93_06230"
     CDS             6938..7309
                     /gene="gcvH"
                     /locus_tag="PKL93_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNQ70897.1"
                     /translation="MKKYAATHEWVSVEGKTATVGISDHAQDHLGDIVYVDLPEVGKS
                     YKKGDVFCTIESVKAASDIYAPVSGKVVEVNEELDSSPEKINDDAEGEGWIAKMEISN
                     ESELDSLMDLEAYKKHCEEEG"
     gene            7311..8657
                     /gene="gcvPA"
                     /locus_tag="PKL93_06235"
     CDS             7311..8657
                     /gene="gcvPA"
                     /locus_tag="PKL93_06235"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNQ70898.1"
                     /translation="MPEFPYLPQTKKDIDEMMEVVGVKSISELFSDIPERFEVDISLP
                     ESKDEFTVLRDLSELSKRNLTLNDMSVFRGAGVYKHFVPSAVQAIASRGEFLTAYTPY
                     QAEVSQGTLQMLFEFQTMICELTGMEVANSSMYDGASAVAEAALMAVRVKGGKKVLVS
                     EALHPEYVETIKTYCFGSDIDVETVAFDSESGQIDVTDLQNKLTEDISGFVLGYPNFF
                     GIIENLSEIRSTIGEHTMMIVSTNPIALGILEAPGMLGADIVVGDGQPLGNAPSFGGP
                     GLGFFASKESYIRKMPGRIIGETKDNDGRTGYVMVLQTREQHIRRSKATSNICSNHAF
                     NALIASIYMSMIGSEGLREIAKRSFDKAHYLSERISRTDHVSLVFTGPFFNEFVARFD
                     CDLKEFNKELLKDKILGPLELERFNEKLKDCGLICTTEANLNEEVEFFAGRLEAIG"
     gene            8654..10102
                     /gene="gcvPB"
                     /locus_tag="PKL93_06240"
     CDS             8654..10102
                     /gene="gcvPB"
                     /locus_tag="PKL93_06240"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNQ70899.1"
                     /translation="MTIFELSKEGRKSFFLPKEESYGYEPSELLPEESVRKNEPLLPQ
                     LSELQAIRHYTGLSKKNHSVDSGFYPLGSCTMKYNPRVNEKIANLDGFSQIHPYQSED
                     TVQGALEVMYNLQNSLCEITGMDSFTLQPAAGAHGELVGMLLMKKYFELKGEDNRRKV
                     IVPDSAHGTNPASAVMAGFEVIEVPSGRNGRVDLKSLEEILDESIAGIMLTNPNTLGL
                     FENEICEIQELAHSKGALLYYDGANLNAIMGHARPGDMGFDIVHLNLHKTFSTPHGMG
                     GPGSGPVGVKSFLADLLPVPVVRFNGRRFSLDYDLPNSIGKVRSFYGNFGVFLKAYAY
                     ILTLGGDGLKRASEMAVLNANYLRARLTKLIPTAYAGICMHEFVLKGSKLVSDYGVKT
                     LDVAKRLLDYGIHPPTIYFPLIVEEALMIEPTETESKESLDIFADTFEKILNEAKKDP
                     EILRAAPKLTAVGRLDEATASRKPKTRWSKDS"
     gene            10146..10222
                     /locus_tag="PKL93_06245"
     tRNA            10146..10222
                     /locus_tag="PKL93_06245"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10180..10182,aa:Thr,seq:ggt)
     gene            10512..11912
                     /locus_tag="PKL93_06250"
     CDS             10512..11912
                     /locus_tag="PKL93_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNQ70900.1"
                     /translation="MFDFYSKPRIILVGRSQKDARRLIEAFDERFDVRYVEEVSDEYS
                     RFSDAMVFDYVGEDIIMNLRSGSIPYLCLIGSEKAIAKYSLEPGEYLLKGPGYLHYLP
                     EIVYSMLEKHRLQKTLDFEREKYMGLVNSMGCGMLILRKRDGNVIFCNQVAQSLLGYT
                     EEEIEKMRLQDLVYDEPFALETILGIEHFDTDREIVMLTKTGGKTYVIFNTSEMLYGA
                     ESVVQLTFMDVSKQKRDREELIFQWRFLDNAEEIAIAIDEKGRIVYLNKFAAEIHGYD
                     LEEMLGDNLASYLVHGEGEAESMQFSMMKSGKWKGRQLHRRRDGTEFIVEAKRSMLRV
                     DDNVYELVIGIDITESIELQRRNNLHSLLLNGTGDLAIATDMENRLIYLNEAAEKYFE
                     TRLEDEENKALEDISSEPLRNFLKIAASKPSEPLKNKIRFPVRQKSNAVIEFTGKIVR
                     DHDEEVGTIFIGRKVT"
     gene            complement(12275..>12862)
                     /gene="nadE"
                     /locus_tag="PKL93_06255"
     CDS             complement(12275..>12862)
                     /gene="nadE"
                     /locus_tag="PKL93_06255"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNQ70901.1"
                     /translation="IDDIMCTTLSTLEPVFTGTSEDITEENLQARIRGMILMALSNKF
                     GWLVLITGNKSEMATGYATLYGDMAGGFAVLKDLYKTQVYRIAERINELAKRDMIPRN
                     VFSKAPSAELREGQKDQDSLPPYEILDEILRLHIEEGQSAEEIVLEGFERDTVEYSLK
                     LLRRSEYKRKQCPPGIKVSKRAFGKDWRMPITNHY"
     assembly_gap    12863..12962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12963..13773)
                     /locus_tag="PKL93_06260"
     CDS             complement(<12963..13773)
                     /locus_tag="PKL93_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNQ70902.1"
                     /translation="MIVRTALAQINTTVGDINGNKNKIIEAIDKATASDSEILLFPEL
                     TITGYPPEDLLLNTGFLRENLAALKEIANYTEGSKTMIVLGFVDFSNEIYNAAAVIHS
                     GEIRAVYRKMSLPNYSVFDERRYFSPGSHPLLVRYGEANIGINICEDLWVPSGPINEQ
                     AIGGANLILNLSASPFSGMKSKTRSALLLTRAMEYSSIIVYCNLVGGQDDLVFDGRSC
                     VAMPDGRLLLGKAFEEDMILLDIDTDVSTRYNLFEGKRKDYSMQVSLEEVSI"
     gene            complement(13770..14678)
                     /locus_tag="PKL93_06265"
     CDS             complement(13770..14678)
                     /locus_tag="PKL93_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNQ70903.1"
                     /translation="MKRLLVFVLVLIEAALVAVPSVFFTDDGRAVQRILNVLEDAQEE
                     VILVSYSLDETEIMKCLNHLERRGVRIEVMIDDSTVDRAFAKSPEFEIRSDTAAALVH
                     SKFLVVDRQVVVFGTGNFTEGSLREDSNSFLVFESPSVAELFLRYFEAIETGVSLDEV
                     NIGNIDFYLCPSQEARKRVIKELMGARSEICFAMFAFTDPEILAALKFCASIGVRVTG
                     IVDSWNEDSPLREYLTSGMEVDEGGAVTIHDKTFVVDGETVITGSANASLSGWGKNRE
                     VVAIIDSRELAKEFVNHFEYIRGVSR"
     gene            complement(14724..14799)
                     /locus_tag="PKL93_06270"
     tRNA            complement(14724..14799)
                     /locus_tag="PKL93_06270"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14764..14766),aa:Ala,seq:ggc)
     gene            complement(14854..15774)
                     /gene="secF"
                     /locus_tag="PKL93_06275"
     CDS             complement(14854..15774)
                     /gene="secF"
                     /locus_tag="PKL93_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNQ70904.1"
                     /translation="MTFKIDFVGKRKYYIALSLVLIVVSVIFIFTKGFNFGVDFTGGI
                     EISVSVPDVDMTVAEMRELLSAEDPNFAAARIIKQRPLTEEGNFEQRSRFSIIVNTSE
                     SEESVTTKILAGLKGKDVTEGNLLSVSTISGFAAQEIRGYAWIAVTVSMALLLLYITI
                     RFKFSFGVGAILSLVHDVIIVMGFYSIFGIEFNAPVVASLLTLVGYSLNDTIVVYDRI
                     RENTKKMRGKTIETVVNTSINDVIVRSINTSLTTFLAVLTLFIFSGEVLRPFAFGMLV
                     GVVVGTYSSLYIASPVVIEWLKRSESRSHA"
     gene            complement(15771..17228)
                     /gene="secD"
                     /locus_tag="PKL93_06280"
     CDS             complement(15771..17228)
                     /gene="secD"
                     /locus_tag="PKL93_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNQ70905.1"
                     /translation="MALLPLLIPHGSAPAGSSFIDKLAANIKLGLDIKGGALLEYQML
                     TDVSDQEMDALADRVIEVLRARLDAAGFTEATVEKVTTGISFGEDIPPVRVRVQIPGI
                     TDVSRAESLVGKTGRLYFADVLAIETSVSTPSIPAGMSLEISRRKLQGAEPYWVKELH
                     YGEYVGGVQNNTWYFISPKVSIGSSYAELDGSVVTDAKPLVNNQPRPGQGRFMVSLKF
                     SSEGAKTFRDITSVKSTYADTDMKKRLAIVLDDRVIIAPLVQSQISDGNAVIEGLNSI
                     EEAKEVSILVGSGNLPVDLASFNKRVLSPTLGRDIINSSLWAGIAGIVIIMIYMVVFY
                     KKMGLVADLALLYNAVLLFGMISLTGSILTLPGIAGIVLTIGMTVDGNVLIFERIKEE
                     LRLGKTPENAIDSAFSKVVWTIFDANLTTILAGLVLYYFGTGTVKGFAITLIIGVIGA
                     MFTNIVVSRTVLAGMAGSLKPHRYVKVETEGRDAR"
     gene            complement(17343..17735)
                     /gene="yajC"
                     /locus_tag="PKL93_06285"
     CDS             complement(17343..17735)
                     /gene="yajC"
                     /locus_tag="PKL93_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNQ70906.1"
                     /translation="MFSILSFIAYAPAGDVAAPSADAGAATSGGFSGIIIWLVLMVAF
                     FYFLIIMPQRKRDKQFKQMMEKLKVGDRIVTAGGIIGKITLIKPDSLRIRTGTGTELD
                     VTRKAIAVVVNKGENDKEEVEPDVNVKQ"
     gene            17894..18448
                     /gene="frr"
                     /locus_tag="PKL93_06290"
     CDS             17894..18448
                     /gene="frr"
                     /locus_tag="PKL93_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNQ70907.1"
                     /translation="MNSLLKDAENRMKKSVEKIVEEYAQMRTGRPSPALLESVKVDYY
                     GVPTPINQIATITTTEDRSLVIKPWDKSVLSQVEKAIFASKLDLTPINDGINIKLNFP
                     VPTTEQREKWVKIARDNAEKGKIAIRNIRRDAIKEARELEKASEMTEDDLKKFEEEIQ
                     ELTNRYIEEMDKAFEKKSKEIMDF"
     gene            18448..19131
                     /gene="uppS"
                     /locus_tag="PKL93_06295"
     CDS             18448..19131
                     /gene="uppS"
                     /locus_tag="PKL93_06295"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNQ70908.1"
                     /translation="MAIPVHVAFIMDGNGRWAKKKGLERIEGHRKGAEVADAASHWCA
                     DLGIRYVTLYAFSTENWRRPKEEVEFLFELMLLYIGSKVRNMLEEGVRMRFMGRLNEL
                     PPKLGDFVFQIENKTSMGKKLDVIVALNYGGRAEIVDAVNEILKIGKEQISEEDIAMN
                     LYLPDVPEPDLIIRTSGEERLSNFLTWQSVYSELFFTGTLWPDFTKEEFHRAVDDYSK
                     RKRRFGALD"
     gene            19131..19985
                     /locus_tag="PKL93_06300"
     CDS             19131..19985
                     /locus_tag="PKL93_06300"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNQ70909.1"
                     /translation="MKKISETGIRIVTAAVVAPFVILCFVNYYSFIGLVSAVVLFSNY
                     EYLKFSLKERNHQSVRIALSGVIPAIIVAYGILLDRFARVESAAPRPELIFAVGVISI
                     TSIVITTVADVRSAKEIIANSVFSLIYIGFDLAFFYHIYLGFGSSMALMSLTSVWLFD
                     TGAYFLGKKFGHIRISPSYSPKKSLEGVIGGYVTSLLFMLLFVSLSKLTGLYNGPDLG
                     ILQFMVLAVVVAVFGTIGDIAESSFKRYHGVKDSGSLLPGHGGMLDRIDGVLFVTPMF
                     YIFLTLLT"
     gene            20007..22607
                     /gene="alaS"
                     /locus_tag="PKL93_06305"
     CDS             20007..22607
                     /gene="alaS"
                     /locus_tag="PKL93_06305"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNQ70910.1"
                     /translation="MTGAEIRESYLKFFESKDHKRLQSASLIPNDPQLMFTVAGMVPF
                     KPIFWGKIEPTYRRVTTCQKCVRTNDIENVGRTPRHQTFFEMLGNFSFGDYFKREAIK
                     WGWEFVTEYLNLPENRLWVSVYLDDDEAFSIWRDEVGIPEKKIIRLGKKDNWWGPAGP
                     SGPCGPDSEIFIDRGHGDNCPDPENCSPACDCGRFLEFWNLVFTEFNQDEEGNLTYLE
                     RKNIDTGFGLERAASILQDVDSNFDTDLFIPIIKKIEEILGVKYKENPASDISIKVIA
                     DHARSVAFMVSDGILPSNEGRGYVLRRILRRAVRHSSLLGYSSPFLYRIVETVADHMG
                     NVYPELRQRLSFTQEITLKEEERFLSTLANGEKRLKSVLVDKKELSGPELFMLHDTYG
                     FPLELVEEIVAGTGVTLDKEGYEREMALQRERAREAMGDREYLSNVEAYSTIFPVTGN
                     SKFTGYSRFEDKSKIVAILKEGDAVETLSKGETGDLIFEKTPFYPERGGQVTDTGLIR
                     TDNASAKVERVFIPYQEMIVHRAFIEEGALHVGDEAWLSVDEDRRKAITRNHTATHLL
                     HAALRKVLGEHVHQSGSLVQPDRLRFDFSHFQPLSTDELNEVEEIVNEEILKAKVVDK
                     AEKSFDEIKGQDVMALFEEKYGDKVRVISVEGFSRELCGGTHVDNTGEIGLFKILSDT
                     SVAAGTRRIEAITGKTSLNYFRNLFNRQKTIREVLEVPEDLIIQRLQGVLNELKSGGQ
                     EIKRLKEKLLSGDSLMKDKKMIVNGTKLLIRRVENAPSSALRNGADVLLSKEGGGIVI
                     VFNELEDSVSFVVKVEKKLTDKFNAGEIARKIARDLGGGGGGRPDFAQAGGKDRSKLQ
                     TLIERIDEYL"
     gene            22594..23004
                     /locus_tag="PKL93_06310"
     CDS             22594..23004
                     /locus_tag="PKL93_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HNQ70911.1"
                     /translation="MNTYNNGNDKRDNLFSFSVSPDQLSLDSLAFLGDAVYTLYFRLN
                     TLPQARRRAGHQHNIVKDYVSASGQRKALEEVADLLDEEEKEIVRRGYNSRGSKKHGD
                     DEDYKMATAFEALIGYLFLEGKTERIRELLERVI"
     gene            23028..23747
                     /gene="rlmB"
                     /locus_tag="PKL93_06315"
     CDS             23028..23747
                     /gene="rlmB"
                     /locus_tag="PKL93_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNQ70912.1"
                     /translation="MRELLKVDSSALKIKRIVFTNQKNVDSQLLELAEESKARGFRIE
                     EMAPEKLANLCREKKHQGVVIDLKQFPYSDSEELLGAIEEQRTATIVLLDQIQDPHNL
                     GAIIRTSVAAGVDAIAVTSSNSAEITPSVVKVSAGLAFKIQIIKIINMAQFEEILKEK
                     GFWIYASDMKGTPYYENEYSEKSAIVFGNEGKGVRRLVKERSDRLISIPMEDTVDSLN
                     VAASAAIILFDRRRQLSLGGE"
     gene            23755..25551
                     /gene="mutL"
                     /locus_tag="PKL93_06320"
     CDS             23755..25551
                     /gene="mutL"
                     /locus_tag="PKL93_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNQ70913.1"
                     /translation="MRIHELPTDVVRKIAAGEVVTGCFSVVKELVENSIDAGASLVEI
                     EIKAGGKEYIMVRDNGSGMTNQEAKLAIKPHTTSKISSIEDLDSLITFGFRGEALSTI
                     ASVSRMRLSTAADSEELGTILEIAGGSVTGQKPFSGSNGTLVEVFDLLYNTPARRKFL
                     KSASIEGRMVTEMIQRFILAFENVDFVYSRDSQTVFDTRGAGSLQDRIALIYPGISTR
                     DLIPVSGNSGEINVSGYVTLPSRTRKNRMGENIFVNGRYVRQFELNYALERGYGETLQ
                     KGNFPFAVLFIDIDPQEVDVNIHPQKLEVKFASTAAVLDEVKRAVRSAIKGAGHFTIN
                     IVHSEENITGSSEERKDASEIYQDREVSNHGFKHPWRNQKVFDNNASDTYTQSRFDRH
                     LFRSLVPAGEREGSPEKEKSGEGKVSFIGVFGERYLIAESEDGLMLIDQHAAHERIIF
                     EALKESKRIAPQNLLMPLELSLERSKMDLIIAREETLKKLGFTFDFVNDKIIMVSIPQ
                     VMGVEGVVDMLEEILDEMRLEGLEDPKTVFDNLLASIACKAAIRTGNRLDIGQAEELF
                     EELRRKNLLVCPHGRPISMVIRLEDLDRYFSR"
     regulatory      25534..25715
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25759..26652
                     /locus_tag="PKL93_06325"
     CDS             25759..26652
                     /locus_tag="PKL93_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70914.1"
                     /translation="MSVLKRALTVVLIVLFAALSAAIDIVDDLGRLVTINEPPERVVV
                     ASPAITKYLEVLDLSSRIVGVTDWDPFALSNEVEKIGNLVPLNLEKIVSLSPDLVMIS
                     GGFQEAEVEKLENIGVNTYVMNPNSFEDIYRNLVVVGNIFGVPERGKASADDFREEVL
                     AIAKDSHKLSQDEKPRVIYIMIAGEVSDIWTCGTGSFVNQAIAFAGGANVAAPYSGNN
                     GWFPVSPEFVLNSQPEIILVPYYYEGGQEEAVNMIVSYKPFEDLPAVKNDRVYPLYDG
                     LTAYANPNFVNLVKTLKEFFF"
     gene            26649..27641
                     /locus_tag="PKL93_06330"
     CDS             26649..27641
                     /locus_tag="PKL93_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ70915.1"
                     /translation="MKRTVLFVLLSTASILGMLWFSSFGTISYSLGEIFEILIGKSSG
                     TASSIYLNLRLPRLLVAFLIGSGLAVGGNGLQLTLKNPLADPYIIGISAGASFGAVLS
                     LFLREVSRVDLNMNSLAFAFALFSSFLTYTISRRGGRIPITTLVLSGVIVSFLFNAAV
                     TFLVVFAWRNVITLHFWSLGSLSGATWNDVFKLTPVIFAQYGIFAVVHRKMVIMAAGE
                     EHAVTVGVRPELLKFLVFFTVSIVSAVAVSTAGLIGFVGLVIPHMIRLAFGADSKLNL
                     PATALLGGLFLVICDTFARTLFQPTELPIGAVTALVGAPVFIFLLRSKEVAKDG"
     gene            27634..28413
                     /locus_tag="PKL93_06335"
     CDS             27634..28413
                     /locus_tag="PKL93_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70916.1"
                     /translation="MVEPIQIEGLHFSYGKEEVLRGIDLHLKNGEVLGVLGPNGSGKS
                     TLMSVICGIRKNWSGKISVMGDNLRDLSTKKLARIVSYIPQSFEPSFDLKVETIVSFG
                     RNPHLKGLGNMTNEDYRIIEESMRLTEVYEFRDRPFSTLSGGEKQRVIIAKALAQEGK
                     ILLMDEFTTHLDPGHARRVGKIATDLVRAKGLSAIAIFHDINQAVEISDRLLFINNGR
                     ALASGKVWDIVTPEIISLVYGLKSRIMENPISGLPLVVFYD"
     gene            complement(28404..28874)
                     /locus_tag="PKL93_06340"
     CDS             complement(28404..28874)
                     /locus_tag="PKL93_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNQ70917.1"
                     /translation="MIPEKVMAILKANDLEFHEFPEGTTPTAITAAQMLKVKVGQIAK
                     SILFICKSGRAYLVVCPGDRKVSLSKLKKIAGEKPRLADETTTLQLTGFSPGSVCPFG
                     LLDINIIVDLNLREYDVVFPAAGTSGSAVETNFEELVRITGAKIADVSNPMSQS"
     gene            29684..30073
                     /locus_tag="PKL93_06345"
     CDS             29684..30073
                     /locus_tag="PKL93_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70918.1"
                     /translation="MRLTVVLLLLCLLSVSFANGLEQTKQTRVELPGCYIIRCYGSCF
                     RGDQGYDCVLTGRAANFDGNGNPLAVTLHLYLDCYVVYFIVQSCSSSGEFSGIGEVEG
                     SVGCFINTGPYEGSCFTYESSCACSDS"
     gene            30113..31357
                     /locus_tag="PKL93_06350"
     CDS             30113..31357
                     /locus_tag="PKL93_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70919.1"
                     /translation="MKRTMLFLILSLCFVTTFAAGLTGYLTQQIPWMAGNEMTLVPLS
                     ESKPDTLEAPSIKGLKYGLLELGAKPIVFALIPGEIPLLWIDANNNSNVLDDPTIAPD
                     FKETHQDTTTYEWITRVKTFYELEGYWESRSVKLLARKTGLTGELEFRYCLYEHMEGL
                     VWAEDGPRKLKLFTLDPKGFYSTDQVYFGVDTDGDGEIALIHDSYEIFLHGEVFSLNG
                     KAYRLGEVSKDGKKVSFEETGYAPTEKPRFLKGDALPIPGVLQTDPIVNAAFFEGSPS
                     LIVLSKVTPATVVEPVYSDCDCISLSAFERFRLDGIIDLAQRYTDLKVLWVLTGKEQV
                     EPEAALLENIYLRDEKALVDFYGFPGEERVFIVDSKGVIAELDSYWVDEASLDTDRPQ
                     NGKLMLNYSDIRKTVEALYKTD"
     gene            complement(31474..32562)
                     /locus_tag="PKL93_06355"
     CDS             complement(31474..32562)
                     /locus_tag="PKL93_06355"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNQ70920.1"
                     /translation="MIRKIATLAVIVVISSVSLVSNPINDVINRIIDFEGFWGAILVS
                     RGDVILHAGGYGMADIERNIPNTPEKKFRIASITKQFTAAAILRLCEDGLMSLSDPVG
                     KFIPEFQNGDIISIEQMLNHSSGIPTMINTQELREEIRKFEVGRTLQEAEIAYISSLP
                     LRFEPGSSFLYSNSAYFLLSVIIERASGKRYDDYLRSNFFEPLGMNNTGYDYNEYDSG
                     WGLPYYCDSYVIDLSSVRPADWVDRRLPGGAGGLYSTVYDLYLWDRALSAGEVLSESF
                     LRLMQSPSNELFEAGYGVFIGNELIDGEYRRVVYHDGDTKGTSTRINRYVDDDIFVVV
                     LSNIEGKDFTALAHELAKAVIVNDMGRD"
     gene            33033..34502
                     /locus_tag="PKL93_06360"
     CDS             33033..34502
                     /locus_tag="PKL93_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ70921.1"
                     /translation="MRRIGLMLMLFIVAVTTLLSQRIVVAVMQDPDFLDPHRAAASGT
                     YEMMFNVFEGLLKPDPNGTVIPAIAESYSISEDGMVYTFNLRSGVKFHDGSEVTVDDV
                     LYSLNRLKGSNGERGLSPDFEKFVSKIEAADDKTVRVKLSTLNTDFLEKFIVAIIPES
                     NENHEKNPVGTGPFRFVEYRPGQRLVIEKFEEYWNPELPKVEEVEFRIIPDNQAAIMS
                     FLAGEVQILPRLDAIQAMALGDRYNLLTAEQNMVQLMAMNHAVSPFDDIRVRRAINHA
                     VDKEEIIEIVAGGYGTKLGSNMSPIMSAYYREGLEDYYEPSVEKAKALLSEAGYPDGF
                     DTKITVPSNYQFHIDTAQVIAEQLKRVGIRAEIELVEWSVWLDRVYTAREYEMTIIGL
                     TGKLSAYEILRRYVSDYPRNFYNYSDSEYDRIISLAIEKTAVEEKASMFKRAQEILTE
                     DAAAVYIMDPNFIVALAPNLHGYTVYPLYVQDMSTLYYR"
     gene            34518..35468
                     /locus_tag="PKL93_06365"
     CDS             34518..35468
                     /locus_tag="PKL93_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70922.1"
                     /translation="MFVYLKKLFAMILTVLLVLLITFVTFEIIPGNPALSRLGVDAQE
                     SQYEALSAELGLDDPLGIRLSRWLKGLFTGDLGISMRYSVPVSELILDRLPVTISLAV
                     MALALIILIGIPLGIVSAKYGERLPGVMITVISQIGMAIPSFWSGIILIYIFGILLRW
                     VSPGGYVPWSEDAYGAFKSLILPAIAIALPSIASVIRYTRNSVMDQMKNDYVRLAMSK
                     GMNLNSVLLRHVLKNALIPIITVLGMITANILGGSIVIEQVFTLPGVGRLLINAITTR
                     DLPLVQGMVLYIAFIVVIINFFVDIIYTVIDPRIRLGQRD"
     gene            35470..36273
                     /locus_tag="PKL93_06370"
     CDS             35470..36273
                     /locus_tag="PKL93_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70923.1"
                     /translation="MNKRRINLWIGTVLISILMLMMVVSLFYTPFPVTEINRLERFEP
                     PSARHLLGTDNFGRDVLSRIMKGSQTAFFVGLVAVSIGSVFGVLIGAISGYFGGIVDE
                     IIMRIVDAMMAFPSILLALMFVSVFGVGLRNTIIAIGIMSIPSFARITRSGFVQHKEL
                     DYVKNAKIKGISNLRIMFRHILPNVLSPIVVAASMGFANAVLAEAALSYLGLGIQPPN
                     PSWGRMLSESQVFITVSPWYAIAPGIFITLLVLGFNFLGDGIRDIRENR"
     gene            36280..37260
                     /locus_tag="PKL93_06375"
     CDS             36280..37260
                     /locus_tag="PKL93_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70924.1"
                     /translation="MNCENEPLLEIKDLKIGFKTPEGPLHAVKGISLEVKKREIVGIV
                     GESGCGKTVTALSIMRLLPDNSFATGSINFSGIDLLSLTEDEARGTRGRDISMIFQDS
                     LSSLNPLMKVGRQIDENLMMHTSLDKAERFSRVLEIMKGIGLPDPERTYGKYPHELSG
                     GMRQRIAIAIAIVCNPDLIIADEPTTALDVTVQAQILELLRKINRESNRSIIFISHNL
                     GVIREICSKVYVMYAGHIVERASAEALFTNPGHPYTLGLIKALPKLESKGKELFDIRG
                     RVPSIADLTQGCPFEPRCDYSFERCRKEFPDLKKIDSDHEVRCFLAGGKL"
     gene            37257..38216
                     /locus_tag="PKL93_06380"
     CDS             37257..38216
                     /locus_tag="PKL93_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70925.1"
                     /translation="MKERILEIQDLTKHFRLRRTSLLRRPGSIKALNGISFSISRGES
                     FGIVGESGCGKSTAGYSIVGLLKPTAGKVLFKGEEVNSMDANSLREFRKNVQLVFQDT
                     SGSLNPRGTVEWILSEPLRAMGMAKKQIRNLVVDSLELVGLGKDLLGRFPHELSGGQK
                     QRIGILNALILDPEVIIADEPVSSLDVSIQAQILNLMNDLQDRLSLTYLFISHDLNVV
                     HYFCDRIAVMYLGEIVELSESEELYRSPLHPYTQSLLSAIPGGSYRKTERIILEGDAP
                     SPMNPPSGCFFHPRCFKRMEICSRVKPKIIEVSKDHFVSCHLY"
     gene            complement(38294..>38761)
                     /locus_tag="PKL93_06385"
     CDS             complement(38294..>38761)
                     /locus_tag="PKL93_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ70926.1"
                     /translation="YIQNYWKGLGRTEFQIGIYLAAGSFLAGLVAMKVQRLSHLLKEK
                     KVLIILPLISVASMWGVALCSNKLPFFMVVTMIEAMLFVAGNDYINKLIPSKSRATII
                     SFASMMYSLVMIVFFPIFGRIADTVSFGVAFISLAVMASCLYVLSMYLLKKIG"
BASE COUNT        10749 a         8550 c         8859 g        10503 t
ORIGIN      
        1 aatgcaggat ggatgtctct cactttgtca tcccgaactt gtctcgggat ccagttttga
       61 tcttatcagc gcatagcggt tcatgggtac gagatcccgt acaggagaaa tatgggatga
      121 cagtatgtga gaattaggga atcaccctga acaggagcac ttcagggcag gctctccctt
      181 cacgtcatcc tgacaatgct cctggtcagg atccctgtcc ctcgcgtaaa aaagacgtga
      241 ggagatcgtc agtccctatt ttcgctctcg tcttctcttg agcgcagagg actctcgaat
      301 attctctttg tcggcttttc caagtgttca gcgggtcttc gttctttagc gcacagcgga
      361 tcttagatcg agatgctgaa acgagttcat gacgcattgt tagattctct gaacgatctt
      421 catcttcata catctattct caaacctctg acctctttat cgattttttt gatcctattc
      481 aaggacggct cttcacagcg atcagcggat ccttgatttt aagagtacac cggtcatcaa
      541 gaaattcatt actctctact ttcgaatttt cgttttctcg acttttacac cattcagtga
      601 ccaactatca gtgcctctaa caagtggatc tttcagctgc aaacggcccc tcgatcaagc
      661 gagaattaat gtcaagaccg catagatgat tgagtcgtgt tcgtttgatg atgatgaccg
      721 attctggaac taagaaactt ttgagctcca gagaagatgt catgggtagt gatcggctat
      781 tcaaaggaga attataggaa gaaactgtga gagaagataa ttcatagagg ctctctcact
      841 tcgttttgtt tatcgtacaa agaagctttt ctacttggca aaaaaaaggg agagcaagct
      901 ctcccttcaa aagatcactt catcaatact caggcatctg tggcattgga gcctggggtt
      961 tttcttctgg cttttctgta agagcggctt ctgttgtaag aagtataccc gcaatggatg
     1021 ccgcattctg aagagcactt cttgtaacct ttgcagggtc tatgataccc actttgaaca
     1081 tgtctgtata gacatcgttc agagcatcgt atccaaaagc tgcatcgtca ctgtgaagaa
     1141 tcttgtcaac gaccactgca ccgtctaaac cggcgttctc gactatctgc cgaatcggag
     1201 catctagggc cttcatgacc accttgatac caatcttgat atcgggattc tccgtgctat
     1261 cgaagagttc ctgaacgggc ttcttcgctc tcgcaagaac aactccgcct ccggctacga
     1321 ttccttcctc tactgcagct cttgtcgctg agagagcatc ctcaattcta tgtttcttct
     1381 ctttgagctc ggtctctgtt gcagcaccga ctttgataac cgccactcct cctgaaagct
     1441 ttgcaagtct ttcctggaga gtttcctttt cgtattcgga agtagtctga tcgatctgaa
     1501 ccttgatctg accaattctc tgcttgatct tttcgggatc tcccttgcca tcaacgatta
     1561 tcgtgtcgtc cttcttgacc tttacaacac ccgcactacc aaggtcatcg atagttgcat
     1621 tttcaagggt gagtccaacc tcttcgctga tcacagtacc acctgtaaga attgcgatgt
     1681 cctgcagcat tgcctttctt ctgtcaccga aaccgggagc cttaacagct actgagtcga
     1741 gagtacctct gagcttattg agaacgagag tggaaagtgc ttcaccttca atgtcttcag
     1801 cgattattac gagtggcttg cctgcctggg caaccttttc cagaatcgga acgagcggtt
     1861 ttactgctga gactttcttg tcggttataa gaataagagg ctctttgatt atagcttcca
     1921 tcttctctgg atcggaaaca aagtaaggag aaatgtagcc tctgtcaaac tgcatacctt
     1981 caacgaactc tacgtatgtg tcgagggtct tggaatcttc taccgttatt actccatcct
     2041 gaccgacctt gtccatcgct tctgcaatga gttcaccaat ctccgagtca ttggcagaaa
     2101 tagctgcaac tgaagcaatg tcctctcttg aagagatctt cttcgataag gacctgatgt
     2161 gagagacggc cttctctgtt gccttctgga tacccttctt catgagtata ggattggctc
     2221 cagcggtaac atttttcaaa ccttccttga tcattgcctg tgcgagaacg gtcgcagtcg
     2281 tggttccgtc accggcaata tcgttcgtct tggaagctac ttcctttacg agctgagctc
     2341 caagattctc gaatttatct tccagatcga tttcctttgc gatcgataca ccatcattgg
     2401 ttatcgtcgg agagccccag ctcttctcaa gaactacgtt tctccctttg ggaccgagcg
     2461 ttatcctaac tgcatctgct accgcgtcta cacctttttc aagcgatctt cgtgcttctt
     2521 cgctgtattt cagaatctta gccataccga ttcctccttc aatcctcgat ctttgccagg
     2581 atatcatcct gatcgatgat tatgtagtcg tcatcatcga tcttgatctc cgtgccggaa
     2641 tacttggaat agaggacctt gtcgttttcc ttaaggtcaa tatcctcgac tttctctcca
     2701 actgcaatta ccttcgcagt catctgtttc tccttcgcag cgtcgggaag aacgatacca
     2761 cctgatgtct tcttctcttc ctcataaggc ttaatcaata atcttgtacc aaggggaact
     2821 actttcataa aaagcctcct ttcgaatttt atgttttagc actcacagtg atactctgct
     2881 aataaatgat aaaacatcgt tccgatttct tcaagagctt tctgtgttaa atgatcgtaa
     2941 acataaaaaa atgagcacgc aagaagacgg ctgttcacat ttttctctgg attctttttg
     3001 ttatcataca gcttctgcct tttgcacagt ttcgagtcag gcaactccat cacgcagttt
     3061 ccagtttgag aatgttcgtg gagttgctag ttccaaacgg aataccggcc gtcagtatta
     3121 tcgtgtcacc ggatttcaca agctttaggc gtctagcaag cggcccggcc actctgacga
     3181 gatcgtctgt cgaggagcca aggcccatta aaactgggcg ggcaccccaa acgaggttca
     3241 acctgtaata cgtctcctca tttggagtaa ctgcaagtat gtgagctctc gggcgaaacc
     3301 cggagacgtt tcttgccgtg tgccccgaga atgtggagct cactattact ttcactttca
     3361 gctcctcact tatatcccat gctgccctgc atatggcatc tgtatggtcg tccgtagata
     3421 tcttttccct tgtccactta agtaattccg gattctgctt aagatacttc tctgcagctc
     3481 tagcagtgag atccataacc gacactgctt ctagaggata cttgccgatc gaagtctcgc
     3541 cagaaagcat tatcgcatca ctaccgtcca gaatggcatt tgtgatatcg gttgcctctg
     3601 ccctcgtcgg taccggattc tcaatcatcg attccagcat ttgagtagca gttattacgg
     3661 gaatgcgttt ttgattaccg tatctaatta tcttcttctg agctaccggg acttcttcga
     3721 ccggtatttc aactcctaaa tcgcctctag caaccattaa tccgtcggag acttccgcaa
     3781 tttgttcaat gttttttaag gcctgttctg tttctatctt cgagactatt ggaatagaac
     3841 ccccaagatc cttaatcact tttttggcat gtaaaacatc ttctgatctt ctcacaaatg
     3901 aaagggcaaa ataatcaacc cgttcttcaa ttccaagttt gatgaacttc ttatccttct
     3961 cggtaattgc aggaatgctt atgtcgattc caggaaggtt tatacctctt ctgtgtgtca
     4021 ctattccacc atttaagacc ttagttttga cttcagaatc ggttatcgat tctactctca
     4081 gttttatctt cccatcgtcc atgagaatca gattccccgg ctggacttct tcgggaagtt
     4141 tcttatagtt cacaccgaca atcttctcgt ccccctcgat atcctcggtt gttaggatta
     4201 tttcactgcc tcttttaatg tccaccgcat cctttttgaa gacgcccgtc ctgatctttg
     4261 gacccgagag atctaataat atggtaaggg gagaattcgc cgacttccga atctccttaa
     4321 gattccttat tcgctccctg tgttcggaaa tgtcgccgtg agtcgtattc agtcttgcaa
     4381 catccattcc ccttttaaga agggttttca aaacctccag agattcggta gccggaccga
     4441 tggtacatac aatcttcgtc ttcctcaatc aaaccacctc attaagagag tattttcgcc
     4501 atttccatca gttccatatc cagttctttt ttgtgtgaaa gcacttcctc aagaggtatt
     4561 cctttgattt tcccaccatt caacgacatc atcattcccg ctttaccttc ttcaagcgcc
     4621 ttaaccgacg catatcccat tctcgaagca agtactctat caaatgcagt gggagagcct
     4681 cccctttgaa tgtggccgag aatggttatt cttgtctcgt atccaatcct gtgttcaaca
     4741 tgtctagcaa ctgtatatgc gcttgaagcc ccttcagcga caactataat acagttgatc
     4801 tttccccttt tcctttcatt ccatatcttc tcggcaatcg cttcgtagtc cactggaagc
     4861 tctggaacta taatggcttc agctccaacg gccataccgg aagcaagtgc tacataccca
     4921 gagttccttc ccataacttc tacaacaaag gccctttcat gtgaagaagc cgtgtccttg
     4981 agcttctgaa tcgtttctac gcaggtattc aaacaggtat cgacaccgat acacatatcc
     5041 gtcatggcaa tatcattgtc aatcgttcca ggaagaccta ttacgggaat tccgttttca
     5101 tccatgagaa gcttggctcc gtttaagctt ccttctccac caataactac aagggcatca
     5161 atcccattgc ctctcaaaag gtcggccgct tttgctcttc cctcttcggt tctgaactcc
     5221 tcacagcgtg agctgcgaag tactgtgccg cccttctcca ttattcctcc cacagaggag
     5281 taatccatat cgatgaattc ctcatcgagc agaccggagt atcctctgcg aattccgacg
     5341 acagctatct catccgtgta agccgttctt accgccgctc ttatcgccgc gttcattccc
     5401 ggtgaatctc ccccgctcgt aaggacacca atcttcttta tcatcattat ccctcccaac
     5461 aacttctaca ctcagcaaga cgaaaacatt ctagcacatt gagcttctaa agtcatcaat
     5521 cagaaaagct tccagaagtt aactaatcta attacttggt gtataatttc tggtgaaccg
     5581 ggaggtgata aaatgaagat cctcaaatcg ggagttcctg aatttgaagc gaagatgttc
     5641 caggagattc tgctcgaaga gggaatcttt tcagaacttg tggattctca ttttgcatat
     5701 accgatagta tttactttgg ttcgggcggt ttagtagaca ttattgtacc agacgaagtc
     5761 tttgatgaag ccgtccagat ctttgaagac ttaaagaaaa cggggaagga ggaaatggat
     5821 gagtgagcta aagagaactc cgctttttga tgagcacgta cgtctaaagg ggaaattggt
     5881 ggattttgca ggatgggaaa tgccgctcca gttcgattct attatcaatg aacacaacct
     5941 tgtgagaaag gtggccggtc ttttcgacgt ttcacacatg ggagagatag aaatcgaggg
     6001 tccagacgcc attcactttt cggactacct ggtgacgaac tccgtttctt cactcaagaa
     6061 cggagctatt gtgtattctc caatgtgcaa tgaaaagggc ggaatagtcg acgatgttct
     6121 agtctacagg ttgagcaatg ctaaaactat gttcgttgta aatgcctcaa acaaagacaa
     6181 ggatttcaag tggattacga gcaataaggg tagtttcgat gtgagaatca aagacgcatc
     6241 ggctgacttt gcgcagattg catttcaagg gccacgcgcg gaggagatcc tgtcggaggt
     6301 atcacaggta aggcttgaga aaatcccatt ctatcacttt gaatacggca gggtgaatgg
     6361 cataaaagct ctcgtatctc gcacgggata cacgggtgag gatggttttg agctatacgt
     6421 tgaccccgaa gctgtagtcg ctttgtggag aaagatactt gaattggggt caagtattgg
     6481 tgtcaaacca atcggcctcg gagcaagaga tacccttaga tttgaggcgg cttacatgct
     6541 ttacggtaac gagctaaacg attacaactc tcctcttgaa gcaggtttga agtggaccgt
     6601 caagatggag aaggatttca ttggcaagga agtacttgag gagcaactgg caaatggaac
     6661 aaagtataaa ttgaagggac tggaactatc aggcaagagc attgccagac acgggttcga
     6721 agtctttgat ggtgaaaaga aaatcggctg gatcacgagt ggtatcttct caccgaccct
     6781 tcagaaatcg ctcgcactcg cttacttgga aaaagaatac tggaaaatcg gttccgaggt
     6841 acaggttgag atccgaggta agaggtctcc tgcaactgta gtaaaaacac ctttctatag
     6901 aggttctgtg aaatcaaaag gttaaggagg agtgtaaatg aaaaagtatg ctgcaacaca
     6961 tgaatgggta tcggtagagg gaaagactgc aactgtagga atttcggatc acgctcaaga
     7021 tcaccttggg gacatcgtgt acgttgacct tcctgaagtc ggtaagtcat ataagaaggg
     7081 agacgtcttc tgcacgatag agtctgtaaa agctgccagt gatatctacg caccagtgag
     7141 cggcaaggtt gttgaagtga atgaagaact cgattcatca ccagaaaaga tcaacgatga
     7201 tgccgaaggt gaaggctgga ttgcaaagat ggagataagt aacgaatctg aacttgacag
     7261 cctgatggat ttggaggcat ataagaaaca ctgcgaagag gagggctaat atgcctgagt
     7321 tcccctatct accccaaaca aagaaagaca tagatgaaat gatggaggta gttggtgtca
     7381 agagcatatc agagctgttc agcgacatac ccgaaaggtt cgaggtcgac atctctttac
     7441 ctgagagtaa ggatgaattc actgtactta gagacctcag tgaactcagc aaaagaaatc
     7501 tcacgcttaa cgatatgtct gtcttccgag gagccggagt gtataagcac ttcgtcccta
     7561 gcgcggttca agcgatagca tcaagaggag aattcctgac tgcttacaca ccttaccagg
     7621 cagaagtttc tcagggcact cttcagatgt tattcgagtt ccagacaatg atatgcgaac
     7681 ttacggggat ggaagttgcc aattcatcga tgtacgatgg tgcaagtgcc gttgcggaag
     7741 ctgctcttat ggctgtcaga gtaaagggtg gtaagaaggt tttggtctcg gaggcacttc
     7801 atcccgaata cgttgagacg atcaaaacat attgctttgg tagcgacatc gatgtagaga
     7861 ctgttgcctt cgattccgaa tctggacaga tagatgttac tgaccttcag aacaaactaa
     7921 cagaagatat ttctggcttt gttctaggtt atccaaactt ctttggaata attgaaaatc
     7981 tctcagaaat tcgctccacg attggggagc acactatgat gatagtgagt acaaacccta
     8041 tcgctttggg catacttgaa gctccgggga tgttgggagc cgatatagtc gtgggcgacg
     8101 gtcaaccgtt gggaaatgcg ccttcttttg gtggaccggg acttggtttc ttcgcatcta
     8161 aggagagtta catcagaaag atgcccggaa gaatcatcgg ggagaccaag gacaacgatg
     8221 gtcgcacagg ctacgtaatg gttctccaaa cgagagaaca acatatcagg agaagcaaag
     8281 caacctcgaa catatgttcg aatcatgctt tcaatgcact aatcgcttca atatacatga
     8341 gcatgatcgg ttctgaaggt ttaagggaga tagcgaagag atccttcgat aaggctcatt
     8401 atctttccga aagaataagc agaacagatc atgtaagcct tgtcttcaca gggccctttt
     8461 tcaacgagtt tgtggctcgc tttgattgtg atttgaaaga gtttaacaaa gagttattga
     8521 aggataagat attgggccca cttgaacttg agaggttcaa cgaaaaactg aaggattgtg
     8581 gtttgatttg tacaactgaa gcaaacctta acgaagaagt ggagttcttt gcaggtaggc
     8641 tggaggcgat aggatgacaa tattcgagct gtcaaaagaa ggaagaaaga gtttctttct
     8701 tccaaaggaa gaatcttatg gttacgagcc ttcagaatta ctgcctgaag aatcggttcg
     8761 gaaaaatgag ccgttgcttc cacaattgag tgaacttcaa gcaatcagac actacacggg
     8821 actctcgaag aagaatcact ctgttgacag cggcttttat ccacttggtt catgcacgat
     8881 gaaatacaac ccaagagtga acgagaagat cgcaaatctt gacgggttca gtcagataca
     8941 tccatatcag tctgaggata cggttcaggg tgctttggaa gtcatgtaca acttgcagaa
     9001 ctccctttgc gagataaccg gtatggacag ctttaccctg caaccggctg ccggggctca
     9061 tggtgagctg gtcggaatgc tgctgatgaa aaaatacttc gaacttaagg gtgaggataa
     9121 tagaagaaag gtaattgttc cagactctgc tcatggaacc aatcccgcat ccgcagttat
     9181 ggcggggttt gaagtcatag aggttccttc aggaagaaat ggcagagtcg atttgaaaag
     9241 ccttgaggaa atcctggacg aaagtatcgc aggaataatg ctgacaaatc ccaatacgtt
     9301 agggctcttt gaaaacgaga tctgcgagat tcaggaactg gcacatagta agggggctct
     9361 gctgtattac gacggcgcga atctcaatgc tattatgggc cacgcaagac ctggtgacat
     9421 gggatttgac atagtgcatc tgaacctcca caagactttc tccacacctc atggtatggg
     9481 tggccccgga agcggcccgg ttggtgttaa gagttttctg gcagatcttc ttccggttcc
     9541 cgtagttcgt tttaatggca gaagattctc actggattat gatttgccta actccatagg
     9601 taaggtgagg agtttctatg gaaactttgg agtcttcttg aaggcatatg catatattct
     9661 cactttaggc ggagacggac taaagagagc aagtgagatg gcggtgctaa atgcaaatta
     9721 ccttcgggca agattgacga agttgatacc tactgcttat gccgggatct gtatgcatga
     9781 gttcgttttg aaagggagta aactcgtctc ggattatgga gtaaaaaccc tagacgtcgc
     9841 taagaggctc ttagactacg ggattcaccc gcctaccatt tatttcccac taatagtcga
     9901 agaagcactt atgatcgagc caactgaaac ggagagcaaa gaatcactag acatcttcgc
     9961 tgacacattc gagaagattt tgaacgaggc aaagaaggat cctgagatac tgagagctgc
    10021 tcctaagctt actgccgtgg gcagacttga tgaagcaacg gcttcaagaa agccaaagac
    10081 aaggtggagc aaagacagtt gatattgaaa cttgaaaaat gctgtgatat catagtttca
    10141 gcagagcagg cgtagcttca atcggtcgag cgccgccttg gtaaggcgga ggttgtgagt
    10201 tcaaatctca tcgcctgctc cattttataa ccggatgatc atccggttat ttcttttgtt
    10261 ggccatgaaa gtactaattt tgaatcttct cccaatctat gctagaataa aaatgtaact
    10321 ttcttttcgt agcataacca tccagttccc tttgcaaggc tttgaacggc attgttagca
    10381 aaacgactgt aacctccggt atgaacatca aagtttagct tatttagaat ggattttgcg
    10441 aattgtttcc tttatgggtg gatgtatagg taactggttt catcggtgcg ttctgttcag
    10501 gggtgaagtc tgtgtttgat ttctattcaa agccacgaat tatcctggtt ggaagaagcc
    10561 aaaaggatgc aagaaggtta attgaagctt tcgatgaacg cttcgacgtg agatacgttg
    10621 aagaagtatc tgacgaatac agtcgctttt ctgatgcaat ggtcttcgat tatgtcggag
    10681 aagacataat catgaatctt cgttcaggtt ccattcctta tctgtgtctc attggtagcg
    10741 agaaggctat tgcgaagtac tctctcgaac ctggagagta tcttctcaag gggcccggtt
    10801 atcttcacta tctccccgag atagtctatt cgatgttgga aaagcatcgt cttcaaaaaa
    10861 ctcttgattt cgaacgagag aagtacatgg gtctggtgaa tagcatgggc tgcggaatgc
    10921 taatccttag aaaaagagac ggaaacgtga tcttttgcaa tcaggtcgcg cagagccttc
    10981 tgggatacac ggaggaagaa atcgaaaaga tgcgattgca ggatcttgtt tacgacgaac
    11041 cctttgcttt ggagactatt ctggggattg agcatttcga cactgatcga gaaatcgtta
    11101 tgctaacaaa aaccggtgga aagacttatg taattttcaa cacttctgaa atgctttacg
    11161 gcgcagagag tgttgttcag ctgaccttca tggatgtctc aaaacagaag agagacagag
    11221 aagaactgat attccagtgg cgttttcttg acaatgcaga ggaaattgca atagccatcg
    11281 atgagaaggg aaggattgta tatctgaaca aattcgctgc agaaatccat ggatatgatc
    11341 ttgaagaaat gctgggagat aatcttgcct catatttggt gcatggtgag ggagaggcag
    11401 aatccatgca gttctcgatg atgaagtccg gcaagtggaa gggaagacaa cttcacaggc
    11461 gaagagacgg aaccgaattc atagttgaag cgaagcgaag catgttgagg gttgacgata
    11521 atgtctatga actcgttatt ggaatagata ttacagaaag cattgagctt cagaggcgca
    11581 ataatcttca ctccctgctt ctaaacggga ctggtgatct ggcgatcgca accgatatgg
    11641 agaatcgact gatttacttg aatgaagctg ccgagaaata ctttgaaacc agactggaag
    11701 atgaagaaaa taaagctctg gaggacatta gttcagaacc tctaaggaac ttccttaaga
    11761 ttgccgcttc aaagccatca gagcccctta aaaacaagat cagatttcca gtcaggcaga
    11821 aatcaaatgc cgtcattgaa ttcacgggga agattgtacg cgatcatgat gaagaagtag
    11881 gcactatctt tattggcaga aaggttactt agtctctttc attcctccat aactgactgc
    11941 ctagcgcaag tgacaatcag tttctcggcg cttaagaagg cgactgtctc tcgaggaatc
    12001 gagatcttga agccttctta cctgagatct cacatcgaaa tctgtattca cctcacaggg
    12061 aaattgcgca tgacagttgt gaattgtagc aaagaagggg taaatataga tgaagcatcc
    12121 gaaaggcaag tgttgtgatc ctgagttcga attaagatta atcgagaatt tcctggacac
    12181 tcccatcata ttggtaaata aggttgatta ttaagataac taaggactac ttagaatcct
    12241 tcgatgattt ttgctgtgtt caaaactgga aggttcagta gtgattggta attggcattc
    12301 tccagtcttt tccgaaagct cttttggaca cttttattcc gggaggacac tgtttgcgtt
    12361 tgtactcgct ccttctcaaa agtttgagcg agtactccac agtatccctc tcaaaaccct
    12421 ccagcacaat ctcttccgcc gactgaccct cttcaatgtg aagcctgagg atttcgtcaa
    12481 gaatctcata tgggggaaga gagtcctgat ctttttgacc ttctctcaac tctgctgacg
    12541 gagctttgga aaacacattt cgcggtatca tatctctctt cgcgagttca tttattcgct
    12601 ctgcgattct atatacctgg gtcttgtaca gatccttgag gacggcaaat ccccccgcca
    12661 tatccccgta aagagtagca taacctgttg ccatctcact cttgttgccg gtaataagaa
    12721 ccagccatcc aaacttgtta gagagagcca tcagtatcat ccctcgaatt cttgcctgca
    12781 gattctcttc agtaatatct tcagatgtcc ccgtaaacac tggttctaga gtgctcagtg
    12841 tcgtacacat gatatcgtct atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnttattgag acctcttcga ggcttacctg catagaatag tctttcctct ttccttcgaa
    13021 aaggttgtat ctagtcgaaa catcggtgtc tatatcgagc agaatcatat cttcttcaaa
    13081 agcctttcct agaagcaatc ttccatccgg catcgctacg cagcttctgc cgtcaaagac
    13141 tagatcatct tgaccaccca ccaggttaca gtatactatg atgctggagt actccatcgc
    13201 ccttgtaagc aacagagcgg accttgtctt gctcttcatc ccggaaaagg gagaagctga
    13261 aagattgagt atgagatttg cgcctccaat agcctgttcg tttatcgggc cggaaggaac
    13321 ccagagatcc tcacagatat ttatcccgat attcgcttcc ccgtatctta ctagtaaagg
    13381 gtggcttccc ggagagaagt atctcctttc atcgaatacc gaatagtttg gcaggctcat
    13441 ctttctatag acggctctaa tctcgcccga atgaatcacg gcagcagcat tgtagatttc
    13501 gttggagaaa tctacaaacc caagaacgat cattgttttg cttccttctg tgtagttggc
    13561 gatttcctta agcgcagcca aattctctct gagaaagcct gtgtttaata ataggtcttc
    13621 aggtgggtag cccgtaattg tcagttcggg gaacaaaagt atttccgaat cacttgcggt
    13681 cgccttatca attgcctcta tgattttatt cttgttgccg tttatatctc caacagtcgt
    13741 atttatctga gccagcgccg ttctgacaat catcttgaga ccccccttat atactcgaaa
    13801 tgattcacaa attcctttgc aagttctctc gagtcgatta tagcaactac ctctcgattt
    13861 ttcccccagc cgctcagaga cgcattcgcc gaaccagtaa tcacagtttc tccgtcaacg
    13921 acaaatgtct tgtcgtgaat tgtgacagca ccgccttcat ctacttccat tccagaagtt
    13981 agatattccc gcaaaggaga atcctcgttc cagctgtcta ctattccagt aaccctcact
    14041 ccaatggaag cgcaaaattt caaagctgct aagatttccg ggtcggtaaa ggcaaacata
    14101 gcgaaacata tctcacttct agctcccata agctccttta ttactctttt ccttgcctcc
    14161 tgtgaagggc acaagtagaa atcaatgttt ccaatattaa cttcgtccaa agagacccca
    14221 gtttcaatcg cttcaaagta tcgtagaaac aactcagcta ccgatggaga ttcgaaaacc
    14281 aaaaagctat tcgaatcttc tcttaggctt ccttcagtga agttgcccgt tccgaagaca
    14341 accacttgtc tgtcaacgac aaggaatttc gagtggacta gagccgctgc agtatcgctc
    14401 ctgatttcaa actccggtga ttttgcgaag gctctatcca cagtcgaatc atcgatcatc
    14461 acctcaattc ttactcccct tctttcaagg tgattgagac atttcattat ctcggtttca
    14521 tcaagagagt aagataccaa gataacttct tcttgcgcat cctcaagaac gttcagtatt
    14581 ctttgcaccg ctctcccatc atcagtgaaa aacacagagg ggactgcaac tagtgcggct
    14641 tcaattaaca ccaagacaaa aacgaggagt ctcttcatcc acatcaccaa aattgaaaaa
    14701 gcggagaact cacgttcccc gcctggtgga gacgaaggga ttcgaaccct cggcctatac
    14761 aatgccattg tatcgcgctc ccaactgcgc tacgtcccca ctctaaaaaa attatactct
    14821 accaggtttc acttttcaag aggtagacaa cgattaagcg tggcttctgc tctctgatct
    14881 cttcaaccac tcgataacga cagggctggc gatgtacaga gaggaatatg ttccaacgac
    14941 cacaccgacc agcattccaa aggcaaatgg tctcaagacc tctcccgaga atatgaagag
    15001 agtgagaaca gcaaggaaag tggtcaaaga cgtgtttatc gatctaacta ttacatcatt
    15061 gatactcgta tttaccactg tttctatcgt ctttcccctc atcttcttag tattctccct
    15121 aattctgtcg taaacgacga tcgtgtcatt aagggaatat ccaaccaaag taaggagtga
    15181 tgcaactacc ggtgcattga actcaattcc gaagatactg tagaagccca taacgatgat
    15241 cacatcatga acaagagaaa gtatcgctcc aactccgaag gaaaacttga atctaatcgt
    15301 tatgtataga agaagaagcg ccatggacac tgttacggca atccaagcat atccccttat
    15361 ctcctgggca gcaaatcccg aaatcgtact taccgaaagt aggttgccct cagttacgtc
    15421 ctttcctttt agaccggcca gtatcttcgt agtgacggat tcttcgctct cggaagtgtt
    15481 gacgataatt gagaatctac tcctctgttc gaaattacct tcttcggtaa gtggtctttg
    15541 ttttatgatc cttgcggctg caaaattggg atcctctgcg ctcaggagct ccctcatctc
    15601 agccacagtc atatcgacat ccgggacgga aacactaatt tcgattccgc ctgtgaagtc
    15661 aacgccaaaa ttgaatcctt tggtgaaaat aaagataaca gagacgacaa tcagaacaag
    15721 ggaaagagca atgtagtact ttctttttcc aacgaagtct attttgaagg tcatcttgca
    15781 tctctccctt ccgtttcaac tttaacgtag cgatggggtt tgagacttcc cgccatcccg
    15841 gcgagcaccg ttctggatac cactatgttg gtaaacatcg cacctataac accaattatc
    15901 aaggtaatgg caaacccttt aactgtcccc gttccaaagt aataaagaac gagtcctgca
    15961 agtatcgtgg tcagattggc atcgaaaatg gtccagacga ctttagagaa agcagagtca
    16021 attgcgtttt ccggtgtctt gcccaacctt aattcttcct ttatcctctc gaaaataagg
    16081 acgtttccat caactgtcat tcctattgtc agaactatac cggctattcc aggcagtgtg
    16141 agaattgaac cggttagaga tatcattcca aagagaagaa ccgcgttata cagtaaggcg
    16201 agatctgcaa caagacccat cttcttatag aaaacaacca tgtagatcat tattataacg
    16261 atacccgcta ttccggccca aaggcttgaa tttatgatat ctctgccaag cgtgggcgac
    16321 aaaactctct tgttgaacga tgcaagatcg acgggaagat ttccacttcc gacaagaatg
    16381 gaaacttcct tcgcttcttc gattgaattc aaaccctcaa ttacggcatt tccatcggaa
    16441 atctgagact gaactagagg cgcgatgatt accctatcgt ccaggacaat tgcgagcctc
    16501 ttcttcatat cggtatcggc atatgtggac ttcactgaag taatgtccct aaaagtcttc
    16561 gcaccttcgg aactgaactt cagagaaacc atgaatcttc cttgcccggg tctgggctga
    16621 ttgttcacca aaggcttggc atctgtaacc acgcttccgt caagctctgc atagctagac
    16681 cctatgctga ctttgggact gatgaaatac catgtgttgt tctgcactcc tccgacgtat
    16741 tctccgtagt gaagttcctt gacccagtaa ggttcggctc cttgaagctt tctcctgctt
    16801 atctccagcg acattccggc tgggatagag ggcgtagaaa ccgaagtttc aatcgcgagg
    16861 acatcagcaa aatagagcct cccggtcttt cccactagac tctcggctct agatacatct
    16921 gttattccag gaatttgaac tcgtactctc acaggtggaa tatcttcccc aaacgaaatt
    16981 cccgtggtaa ccttttctac tgttgcttca gtgaatccgg cagcatccaa tcttgccctc
    17041 aaaacttcaa tgactctgtc tgcaagtgca tccatctctt gatcagaaac atcggtaagc
    17101 atctggtact caagaagagc tccacccttt atatcaagcc ctaacttgat gttcgcagcc
    17161 aatttatcta tgaagctgct tccggctggt gcactaccgt gaggtataag cagtggcaat
    17221 aatgccacaa caaacaccac aacggtaacg atcaagcgtt tttggtttgc tctcatacgg
    17281 actttgcccg aaagactcgg acagacaccc ccttccaata tctacaaatg tacctgaaac
    17341 tactactgtt tgacatttac atcgggttct acttcttctt tgtcattctc gcctttatta
    17401 actacgacgg cgattgcctt tcttgtaaca tcaagttctg ttccggtgcc ggtcctgatc
    17461 cttaggctgt caggttttat cagtgtaatc tttccgatga ttccgccggc cgtaactatt
    17521 ctatcgccaa ccttgagctt ctccatcatc tgcttgaact gcttatccct ctttctttga
    17581 ggcataatta tgaggaaata gaagaaagca accataagaa ctagccatat gattattcct
    17641 gagaagcccc cgctggttgc tgcccccgca tctgccgaag gagctgccac atctcctgcc
    17701 ggggcatatg caatgaaact aaggattgaa aacatttaca tacctcctgt catggttaga
    17761 gcacctgtat gattttacca gaatagacta cccaattcaa ctaacaaacc ttttttgcag
    17821 ttctgacgct actaatacga aaagccttac atgatataat tccgttgaca tacgagagaa
    17881 agggaggaag atcatgaata gcctgctgaa agatgcagaa aaccgaatga agaagagcgt
    17941 agaaaaaatc gtggaagagt acgcccaaat gcgtacgggc agaccttccc ctgcccttct
    18001 tgagtctgta aaggtcgact attacggagt gcctacaccg attaaccaga tagccacaat
    18061 cacaactacc gaagaccgtt ctttagtcat taagccctgg gataaaagcg ttttgagcca
    18121 ggttgaaaaa gcaatctttg cctctaagct tgacttgact cccataaacg acgggatcaa
    18181 tatcaagttg aactttcctg taccgacgac ggaacagaga gaaaagtggg tgaagattgc
    18241 aagagataat gcagagaagg gaaagatagc tattagaaac atccgaagag acgctataaa
    18301 ggaagctaga gaacttgaaa aggcatcaga gatgactgaa gacgatctta agaaattcga
    18361 ggaggaaatt caggagctta caaatcgata tattgaagag atggacaagg cttttgaaaa
    18421 gaagagtaag gagatcatgg acttttaatg gccattcccg ttcatgtagc ctttataatg
    18481 gatggaaatg gccgatgggc caagaaaaag ggacttgaga gaattgaagg acacagaaag
    18541 ggtgctgaag tagcggacgc cgcctctcac tggtgtgcag accttggaat aagatacgta
    18601 acactttacg cgttttccac ggagaactgg aggcgtccaa aggaagaagt cgaatttcta
    18661 tttgaactga tgctccttta cataggcagc aaagttcgca atatgctgga agagggagtt
    18721 agaatgcgat tcatggggag gctgaacgag cttcctccta aacttgggga cttcgtcttt
    18781 cagatcgaga acaagacttc gatgggtaag aaacttgatg ttatcgtagc ccttaattat
    18841 ggtggaagag cagagattgt tgacgcggta aacgagattc ttaagatcgg aaaggaacaa
    18901 atatcggaag aagacatagc catgaatctt tatcttccag atgttcccga acccgatctg
    18961 atcattcgaa cctctggaga agaaagactt agcaactttt tgacgtggca atcggtatac
    19021 agtgagctgt ttttcactgg aactctgtgg cctgatttca cgaaagagga attccataga
    19081 gcagtggacg attactcaaa aaggaaaaga aggtttggtg cattggactg atgaagaaaa
    19141 tctcggagac aggtatcagg atagtgaccg ctgccgttgt ggctcctttt gttattctat
    19201 gtttcgttaa ctattattca ttcattggat tggtctctgc cgtggtgctt ttttcaaact
    19261 atgaatacct gaagttcagt ttaaaagagc ggaaccatca gtcagtcaga attgctctgt
    19321 ccggggtaat acctgcgata atcgttgctt acggaatact tctcgataga tttgccagag
    19381 ttgaatctgc agctcctaga ccggagttga tttttgcagt gggtgtgatt tctattactt
    19441 cgatagtcat tactacagtc gccgatgtaa ggagtgccaa ggagataata gctaattctg
    19501 tattctccct tatatacatt ggtttcgatc ttgcattctt ttatcacatc tatctcggct
    19561 tcggctcatc tatggcgttg atgtcattaa catctgtgtg gctattcgac accggagctt
    19621 actttttagg aaagaaattt ggtcacatca ggatctctcc gagttacagc cccaaaaaga
    19681 gtctcgaggg ggtaattggt ggatacgtga cttctcttct ttttatgctt ttgttcgtct
    19741 ctttgagcaa actaacagga ctttacaacg gtcccgacct gggaatactt cagttcatgg
    19801 tattggcagt tgtggtagcc gtatttggta caattggtga cattgccgaa tcatctttca
    19861 aaagatatca cggtgttaag gattcgggca gtttgctgcc tggacatggt ggtatgcttg
    19921 atcgtataga cggtgttctt ttcgtaacac ccatgtttta tattttcctt actcttttga
    19981 cttgaggagg caagatagtg aaatacatga ctggcgctga gataagagag agttatctca
    20041 agttttttga atctaaagat cacaaaaggt tgcagagtgc ttcattgata ccgaacgatc
    20101 cccagctgat gttcacagtc gccgggatgg ttccttttaa accgatcttc tggggcaaga
    20161 tagaacccac ttacagaaga gtaacaacct gtcagaaatg cgtgaggact aacgacatcg
    20221 aaaacgtggg aagaacccct agacatcaaa cgtttttcga gatgctgggt aacttctcat
    20281 tcggcgatta ttttaagaga gaggccataa aatggggttg ggaatttgtt accgagtatc
    20341 tgaatctccc ggaaaaccgt ttgtgggttt ccgtctatct tgacgatgac gaagcgtttt
    20401 cgatatggag agatgaagtc ggcatcccag aaaagaagat cattaggctt ggaaagaagg
    20461 acaactggtg gggtccggca gggccctccg gtccatgtgg acccgattca gagatcttca
    20521 tagacagagg tcatggagac aactgcccgg atcccgagaa ctgcagcccc gcttgcgatt
    20581 gcggtagatt tcttgaattc tggaatcttg tatttacaga attcaaccag gacgaggagg
    20641 gaaatctcac ttacctcgaa agaaagaaca tcgatacggg atttggcctt gaaagggctg
    20701 cctcgatact tcaagatgtc gattcgaact tcgacaccga cttgttcata ccgataatca
    20761 aaaagattga agagatcctt ggagtcaagt ataaggaaaa cccagcctcc gatatctcga
    20821 taaaggtcat tgccgatcat gcgagatcgg ttgcatttat ggtttcagat ggtattctcc
    20881 cctctaacga aggccgtgga tacgtactaa gaagaatttt gagaagggct gtacgccaca
    20941 gttcactgct cggatacagt tccccgtttc tttaccgaat agtggagaca gttgcggatc
    21001 atatgggtaa tgtatatccc gaactgaggc agagactttc attcactcaa gaaatcactc
    21061 ttaaagagga ggagagattc ctctcaacac ttgcaaatgg tgaaaagaga ttgaagtcag
    21121 tacttgtcga caagaaggaa ctttcaggtc ccgaattatt catgctacac gatacttatg
    21181 gattccctct ggaacttgtt gaggagatcg ttgcgggtac cggtgtaact cttgacaaag
    21241 aaggctatga aagggaaatg gcgttacaga gagagagggc cagggaggca atgggagaca
    21301 gagaatatct tagcaacgtc gaagcatatt caaccatatt tcctgtgacc ggcaattcga
    21361 aattcacagg ttactcgaga ttcgaagaca aatccaagat cgtcgccatt ctaaaagaag
    21421 gtgacgccgt agaaaccctt agcaaaggcg aaaccggaga tttaatcttt gaaaaaacac
    21481 cgttctatcc cgaaagggga ggacaggtga ccgatacggg tttgatccgt acagacaatg
    21541 cttctgcaaa ggtcgagaga gtgttcatac cttaccagga gatgatagtc cacagggctt
    21601 ttattgaaga aggtgcttta catgttggag acgaagcttg gctttcggtg gatgaggacc
    21661 gaagaaaggc gataacgaga aatcacaccg ctactcattt gctgcacgca gcgttaagaa
    21721 aggtgctcgg ggaacacgtc catcaatctg gttctctggt tcagccagat agattacgtt
    21781 tcgatttttc gcattttcag ccgttatcaa cagacgaact caacgaagtt gaagaaatcg
    21841 taaatgaaga gatcctcaag gctaaagtag tagataaggc agaaaaaagc tttgacgaga
    21901 ttaaagggca ggatgtgatg gcgctttttg aagagaagta cggcgacaag gtgcgggtaa
    21961 tatcagtcga aggattcagc agagagcttt gcggaggcac tcatgttgac aacaccggag
    22021 aaatcggtct cttcaaaatc ctttccgata cctctgttgc tgcaggaaca aggcgaatag
    22081 aagcgataac aggaaaaacg tctcttaatt atttcagaaa tctgttcaac aggcagaaaa
    22141 caataagaga agtgcttgag gttcccgaag atttaattat ccagaggctt caaggggttt
    22201 tgaacgagct gaagtcaggt ggacaagaga taaaaagatt gaaagaaaag ctgctctctg
    22261 gagactccct gatgaaagat aagaagatga tcgttaatgg gacgaaactg ctcataagaa
    22321 gggtcgaaaa cgctccttct agcgccctga gaaatggggc cgatgttctt cttagcaaag
    22381 aaggcggcgg aatagtgata gtattcaacg agctggaaga ctcagtctcg ttcgttgtga
    22441 aggtcgagaa gaaacttact gacaaattca acgctggtga gatcgccagg aaaatcgcaa
    22501 gagaccttgg agggggcggt ggaggtagac cagactttgc ccaggcaggt ggaaaggatc
    22561 gatcaaagtt gcaaacatta atcgagagga ttgatgaata cctataacaa cggtaacgac
    22621 aaaagggata acctcttttc tttctcggta agtcctgacc agttgtcgct tgacagcctt
    22681 gctttcttgg gagatgcagt ttatacgctc tattttcgat tgaatacctt gccgcaggcc
    22741 cggaggagag caggtcatca gcacaacata gtaaaagatt acgtaagtgc ctcaggtcaa
    22801 aggaaagctc ttgaagaagt tgctgacctt cttgatgaag aagagaaaga aatcgtcaga
    22861 aggggttaca attccagagg ttcaaaaaag catggagatg atgaggatta taaaatggcc
    22921 accgcttttg aagctctcat cggctatcta ttccttgagg gaaagactga gagaatcaga
    22981 gagctactcg aaagggtgat ttgatgtatc tacatggaag aaacgtattg agagaacttc
    23041 tcaaagtcga ctcttcagcg ttgaaaataa agcgcatagt tttcactaat cagaaaaatg
    23101 tcgactccca gcttcttgaa ctggctgagg aatcaaaggc aaggggattc agaattgaag
    23161 agatggcacc agaaaagctt gccaatctct gtagagagaa gaagcatcaa ggcgttgtga
    23221 ttgatcttaa acagtttcct tactctgact ccgaagagct cctcggagca attgaagagc
    23281 aaaggacagc tacgattgta cttcttgatc agattcagga tccgcataat cttggtgcga
    23341 taattaggac ttcagtggca gctggggtgg atgctatcgc agttacctcg agtaactccg
    23401 cggaaataac cccgtcggtt gtgaaagtct ctgccggtct cgctttcaaa attcagataa
    23461 taaagataat aaatatggca cagttcgaag agattctgaa agagaaagga ttctggattt
    23521 atgcttcaga tatgaaaggt accccttatt atgaaaacga atactctgaa aagtctgcta
    23581 ttgtgttcgg aaatgaaggg aagggtgtaa ggcgactggt aaaggaaaga agtgaccgtc
    23641 tcatttcgat tcctatggaa gatacagtgg actcccttaa tgtagccgcc tcggccgcaa
    23701 ttattctctt tgacaggcgt cgccagcttt ccctgggagg agagtgagct ttttatgaga
    23761 attcacgagc tcccaacaga tgttgtgaga aagatcgcgg cgggagaagt ggtaaccggt
    23821 tgtttttcgg ttgtgaagga acttgtagaa aacagtatag atgcaggagc atcgcttgtt
    23881 gaaatcgaaa taaaagctgg tggcaaggaa tacataatgg ttagagacaa cggtagtggg
    23941 atgactaacc aggaagcgaa actcgcaata aaaccccata ctacgagtaa gatctcctcg
    24001 atagaggatc tcgactcgct cattacgttt ggctttagag gcgaagctct ctcgacaatt
    24061 gcttcagttt cacgcatgcg tctttcgacc gcagcagatt cagaagaact tgggacgatc
    24121 ctggaaattg caggaggaag tgtcactggt cagaaaccct ttagcggctc aaatggcact
    24181 ttggtggagg tctttgattt gctttacaac acacccgcca gaaggaagtt cttgaagtcc
    24241 gcctcaattg aaggtcgtat ggtaactgag atgatccaga gatttatttt agcttttgaa
    24301 aatgtcgatt ttgtgtattc ccgtgattct caaacggtat tcgacacaag aggagccgga
    24361 tctcttcagg ataggatcgc tctcatctat ccagggatct ctacgaggga tctgattcct
    24421 gtctcaggta attcaggcga aatcaatgtc agtggttacg ttacactccc tagccgcacg
    24481 agaaaaaaca gaatgggtga aaacatcttt gtgaacggga gatatgtgag acaattcgaa
    24541 ctgaattatg cactggaaag aggttatggc gaaacccttc aaaagggtaa ttttcctttt
    24601 gcagttcttt ttattgatat tgatcctcag gaagtcgatg ttaacatcca tccacagaaa
    24661 ctcgaagtta agtttgcttc tactgcagcc gttctggatg aggtgaagag agcagtgcga
    24721 tcggccatca aaggtgccgg tcatttcaca attaacatag tccatagcga agagaacata
    24781 accggaagtt ctgaggaacg gaaagatgcc tcagagattt atcaggatcg cgaagtaagt
    24841 aatcacggct tcaaacaccc atggaggaac caaaaagtct tcgataacaa tgctagcgac
    24901 acctacacac agtccagatt tgatcgtcac cttttcagat cactagttcc tgctggagag
    24961 cgtgaaggtt caccggaaaa ggaaaaaagt ggggaaggaa aagtcagttt tattggtgtt
    25021 ttcggagaaa ggtatttgat tgcggaatcc gaggacggtc tcatgttgat cgatcagcat
    25081 gccgctcacg agcggatcat tttcgaggcc ctcaaagaaa gcaagagaat cgccccgcaa
    25141 aatcttctaa tgcctcttga actgagcctc gagaggtcaa aaatggatct aattatcgca
    25201 agagaggaaa cgctgaagaa actcgggttc actttcgatt tcgtcaatga taaaatcata
    25261 atggtctcca ttcctcaggt catgggagta gagggcgtag tcgatatgct agaggagatt
    25321 ctcgacgaaa tgaggcttga aggtcttgag gatccgaaga cggttttcga taatctcctg
    25381 gcatcgattg catgcaaggc tgcaataagg acagggaaca gacttgacat aggacaagcg
    25441 gaggagctct tcgaagagct gagaagaaag aatcttcttg tttgtcccca tggaagacca
    25501 atctccatgg taataagact ggaggatctg gacaggtatt tctccaggta actctgaaaa
    25561 acatagcttc atatcaaggg aaaccggtgt gaatccggta cggtcccgcc actgtgagcg
    25621 gtacgaactc tgcgagtgcc actgggtaac cgggaaggcg cagatattag gttgatccgc
    25681 aagtcaggag acctgtgtga agctcagtta tcaccatcct tccgaggtag aggggtggtg
    25741 aaattccttg caaaagaggt gagtgttttg aaaagagctt taacagttgt tttgattgta
    25801 ctgtttgccg ctctgtcagc tgcgatcgac atcgtggatg atcttggaag actcgtaacg
    25861 atcaatgaac ctcccgaaag agtggttgtc gcttctcccg cgataacgaa gtatcttgag
    25921 gttcttgacc tttcatccag aatcgtagga gtaaccgact gggatccatt tgcactttcg
    25981 aacgaagtag aaaagatcgg caatctggtt cccttaaatc tggagaagat agtctcactc
    26041 agtcctgatc ttgtgatgat ttcgggaggg tttcaggagg cagaagttga gaaactggag
    26101 aatatcgggg tcaatacata cgtgatgaat ccaaactcct tcgaggatat ctacagaaat
    26161 ctcgtggttg ttgggaatat ttttggcgtt cccgagagag gcaaggcctc cgccgatgat
    26221 ttcagagaag aggttctcgc aatcgcaaaa gactctcata agctatctca agatgagaaa
    26281 cccagagtaa tttacataat gatagccggg gaggttagcg atatatggac atgcgggaca
    26341 ggttcctttg tgaatcaagc aatcgcattc gcaggcggtg cgaacgttgc cgctccatac
    26401 agtggaaaca acgggtggtt tccagttagc ccagagttcg tgctgaattc tcagccggag
    26461 ataatactgg ttccgtacta ttacgaaggt ggacaggaag aagctgtcaa tatgattgtc
    26521 tcatacaaac cctttgaaga cctgccagct gtgaagaacg atagagtcta tcccctttat
    26581 gacggactta cagcttatgc aaaccccaat tttgtcaatt tagttaaaac gcttaaggag
    26641 ttcttctttt gaaacgaact gtattatttg tactactttc cacagcatcc attctaggga
    26701 tgctgtggtt ttcgagtttc ggcacgataa gctattccct cggtgaaatc ttcgagatac
    26761 tcatcggtaa atcttcggga actgcctcca gtatctatct gaatctgagg cttccaaggt
    26821 tattggtggc attcctgatc ggttcgggac tggcagtggg tggaaacggt cttcagttaa
    26881 ctcttaaaaa tcctctggca gatccttaca ttatcggtat atctgcaggg gcttcttttg
    26941 gagctgttct ttctctattc ttgcgggaag tgagcagagt agacctcaat atgaactcgc
    27001 ttgcttttgc attcgccctg ttttcatctt tcctgactta taccatctcc agaaggggag
    27061 gaaggattcc aattacaaca ctcgtcctat ctggggtaat agtctctttt ctctttaatg
    27121 cagcagtaac atttctcgtc gtttttgcgt ggcgaaacgt aataactcta catttttggt
    27181 ccctgggttc gctgtccgga gctacatgga atgacgtctt caaattaact ccagtcattt
    27241 ttgcccagta cggaatattt gcggtagtcc acaggaaaat ggtaattatg gcggctggtg
    27301 aagaacacgc agttaccgtc ggtgtccgtc ctgaattact caagtttctc gtctttttca
    27361 cggtttccat agtatcggcc gtcgccgttt caactgcagg gttgataggt ttcgtaggtc
    27421 tagtcattcc acatatgata agacttgctt tcggggcgga cagtaaactg aaccttcctg
    27481 caactgcgct gcttggaggg ctgttcttgg tgatttgcga tacttttgca agaacactgt
    27541 ttcagccaac agagcttcct ataggtgcag ttacagcgct tgtgggagcg cccgtattca
    27601 tcttcttgtt gagaagcaag gaagtggcta aagatggttg aacctataca aatagagggg
    27661 ctccatttca gctatggcaa agaggaggtc ttgagaggaa tcgatctcca tctaaagaat
    27721 ggtgaagtac ttggagttct ggggccaaat ggaagcggta aatcaaccct tatgagcgta
    27781 atctgcggaa ttcgaaagaa ctggagcggc aaaataagtg tgatgggcga taaccttcgg
    27841 gacctctcga caaaaaaact ggcaagaata gtatcttata ttccacagag ctttgaacca
    27901 tcatttgatc tgaaagttga gactattgtt tcctttggaa gaaatccaca tttgaaggga
    27961 ctgggaaaca tgacaaatga agattacagg atcattgagg aatcgatgag gcttacggaa
    28021 gtatatgagt tcagggatcg tcccttcagt acgctcagcg gaggtgagaa acagcgtgta
    28081 ataattgcaa aggctcttgc ccaggaaggg aagatacttc ttatggacga gttcacaact
    28141 cacctagacc ctggccacgc aagaagagtt ggaaaaatcg cgaccgatct cgtccgcgca
    28201 aaaggactaa gtgcaatagc gatctttcac gatataaatc aggcggtaga aatttcagac
    28261 aggcttttat tcatcaataa tggaagagca cttgcttcag gcaaggtatg ggatatcgtt
    28321 actccggaaa ttattagttt ggtttacggc ctcaaatcaa gaatcatgga aaacccgatc
    28381 agcgggctgc cactggtagt attctacgat tgagacatgg gattgctgac atcggctatc
    28441 ttggctccag ttatccttac aagctcttcg aagttcgtct caactgcaga accagaagtc
    28501 cccgcggcag ggaatacgac atcatattcc cgtagattaa gatcaacgat aatattgata
    28561 tccaaaagtc cgaaggggca aacacttccg ggagaaaagc ctgttaactg aagtgttgtg
    28621 gtttcatctg caagcctcgg tttttcaccg gcgattttct tgagcttcga aagcgacacc
    28681 ttccgatcac ccgggcagac cacaagataa gctcttccac ttttgcatat gaagaggatc
    28741 gactttgcta tctgaccaac tttcaccttc aacatctgcg cagccgtgat ggcagtgggt
    28801 gtcgtccctt caggaaactc gtggaactct agatcgttgg ccttcagtat ggccatcacc
    28861 ttctctggaa tcatcgtaca cctccgggaa aggaattgaa ttatgatacc acgaatttga
    28921 gagtcaaatc gagcatccga tatgcagata atgaagtcaa aatctaactc aaagggaggt
    28981 gttcttaatc tccttcatct cgacttcgag tagatttcat tttgatgcat catctctttc
    29041 ttgagtagat ttaattatga tgcattacgg catctatcac aaaagtagaa atggaaaatc
    29101 ttttaggaaa agctttcccc tgaataaagg aaacattcaa tgttttctgc taccttccga
    29161 ttcggaccaa ctgcaagccg ttttcgtaat tttggacatc aaagccgggc atatggatgc
    29221 ataatcgatc acattctcat gaacagtcgc caagcgatag cttacttaaa tttattttca
    29281 ctctcttcat taaagaccgg gtcactctct cgatcagaat gcaatcagat taacaacgtg
    29341 cgtgtttcta cagagttttt tgacagccag ccacaaccta gaatttcacg aaccgtcaat
    29401 cttcttcact gcgacaatcc agcctcttcg cagaaccaat gccgtcatac agaaatccgc
    29461 gcgccaaaaa aatcagaacc tgctgaacct ttccaaccgc ctcgagcgga aaagcttggt
    29521 ctcccgatcg ccttcatcgc tttgttggca gtacttcacc aatataatga aatgtattta
    29581 tagagattcc gtattgcctt taatttacaa tacggtattg tggtaaacta ttggtgtaca
    29641 tactcatgtg ccctatccaa attcaatcat tggaggtggt attatgagac ttacggtagt
    29701 tctacttctg ctctgccttc tatcagtctc tttcgcaaac ggtctggagc aaacaaaaca
    29761 gacgcgtgtc gaacttccgg gttgttatat cataagatgc tacggaagct gtttccgggg
    29821 cgatcaggga tacgactgcg tactaacggg cagagcggcg aacttcgatg gcaacggaaa
    29881 tccactggcc gtaacattgc atctctatct agattgttac gtcgtttatt tcatcgttca
    29941 gtcctgcagt tccagcggtg agttttccgg catcggagag gttgaaggtt cggtgggttg
    30001 tttcataaac accgggcctt acgaaggctc ttgcttcact tatgagtcat catgtgcctg
    30061 ttcggactca taaagtttct ccgaaatgtc acagataact ggaggtgatt ttatgaaaag
    30121 gacaatgctt tttctaatac tttctctgtg tttcgtcact acctttgccg ccggccttac
    30181 aggctacttg actcaacaga ttccatggat ggccggaaac gaaatgacgc tcgtcccgct
    30241 cagcgaatca aaacctgaca ctctggaagc tccatctata aaaggattga agtacggcct
    30301 tctcgagctc ggcgccaagc cgatagtatt cgcgctcata cccggagaga tccccctcct
    30361 gtggatcgac gcaaacaaca acagcaatgt gcttgacgac ccgacgatcg caccggactt
    30421 caaggagacc caccaagaca ctacgaccta cgagtggatt accagagtca aaacctttta
    30481 tgaactcgag ggttattggg aatcaagatc tgtcaaactg ttagccagaa agacggggtt
    30541 gactggcgag ttggagttcc gatactgttt gtatgagcac atggaggggt tagtctgggc
    30601 cgaagacggc ccgagaaagc tcaaactctt cacgctcgac cccaaaggtt tttactctac
    30661 cgaccaggtt tacttcggag tcgatacgga cggcgacggc gaaattgctc taatccacga
    30721 ctcttacgag atcttcctgc atggggaagt cttctctttg aacggcaagg catacagact
    30781 tggcgaagtt tccaaagacg ggaaaaaggt ttccttcgag gagaccgggt acgctccgac
    30841 ggagaaaccg agatttctca agggagatgc tcttccgatt cccggagttc tccagacgga
    30901 tccaattgtt aatgcggcct tttttgaggg atcccccagc ctgatcgtcc tcagcaaagt
    30961 cacgcctgcg acagtcgtcg aaccggtata ctccgattgc gactgcattt ctctgagcgc
    31021 tttcgaaagg ttcagattgg acggaatcat cgatctcgcc caaaggtaca cagatctgaa
    31081 ggttctatgg gtactcacag gaaaggagca agtcgagccg gaagcggcgc tgctggaaaa
    31141 tatctatttg agagacgaaa aagccctggt cgatttctac ggcttcccag gcgaggagag
    31201 ggttttcatc gtcgactcga agggcgttat cgccgaacta gacagctact gggtcgacga
    31261 ggcgagcctc gacactgacc ggccgcagaa cggaaagttg atgctcaatt actccgacat
    31321 aagaaagact gtagaagctc tgtacaaaac cgattgaaga tttaaaccgt tagcaaatcg
    31381 agtcttcaga taaggcagag aaccgctctt caaatgaatg agagcggttc tttccattta
    31441 aaacttccga caaaaaacgc tcaacccgcc gaatcaatcc cttcccatat cgttcactat
    31501 aacggctttc gccagttcgt gcgcgagagc ggtgaaatcc tttccctcga tgttgctcag
    31561 caccaccaca aagatgtcgt catcgacata ccgatttatc ctagtcgagg taccctttgt
    31621 gtctccgtcg tgataaacaa ctcttctgta ctcgccatct ataagctcgt taccgatgaa
    31681 gactccatag cctgcctcaa agagttcgtt tgatggactc tgcattagtc ttaaaaagct
    31741 ttctgaaagc acttcgccgg cggaaagcgc tctgtcccac agatacagat catacacagt
    31801 ggagtataac cccccggctc cgccaggaag ccttctatca acccagtcag caggtcttac
    31861 gctagagaga tctataacat aggaatcaca gtagtaagga agaccccatc cgctatcgta
    31921 ttcattgtaa tcgtaaccgg tattgttcat tcccagtggt tcgaagaagt ttgatctcag
    31981 ataatcatca tagcgcttac cacttgccct ttctatgatg acactaagca gaaaataagc
    32041 gctattgctg tagagaaagc tgcttccagg ttcaaacctc aaaggcagag aggagatgta
    32101 ggcgatttcg gcctcttgaa gagttcttcc cacttcaaat tttctgattt cctcacggag
    32161 ttcttgcgtg ttaatcattg tggggattcc agaagaatga ttaagcattt gttcgatcga
    32221 aatgatatcg ccgttttgaa actcaggtat gaacttccca accgggtctg aaagagacat
    32281 aagaccatct tcacatagcc tcagaatcgc cgccgcagtg aactgctttg ttatagaggc
    32341 aattctgaat ttcttctcag gagtattggg aatatttctc tcgatatcgg ccatgccata
    32401 gccgcctgca tgaaggataa cgtctcccct ggagacaaga atggcccccc aaaaaccctc
    32461 aaagtcgata attctgttta tcacatcatt tatgggattg gaaaccagag atacagaaga
    32521 aatcacgaca attactgcta atgtagctat tttccttatc aacgagcacc tcccacaata
    32581 agagaactat cgagtataac tacataataa cctatgacag accatctcag atagagttat
    32641 cgggagattt cactgagatg gtagcgtccg gctttttcac agtttctatg gacattctta
    32701 ctctggtttt tccgaagttt gaaatcaaca catgtagttc tgaagaagcg gtctcggcga
    32761 cctaaatacc taggactacc tacgagtaga caatgaagat tgatgagctg gataagaact
    32821 acaagaatct tcgcataaag cagatcaatg taatctgcga tagactattg atgaaacgga
    32881 aagatttgca aattatcccg gaatatttct caacaaagcc agagttaggt ttttccactc
    32941 ataatgttta aaatatcaat cgatgctggc tttgctgtat attacgcccg gattgaatga
    33001 tgcactgatg ggttcgtttg gggaggtatg aagtgagaag aattggctta atgttgatgt
    33061 tgttcattgt tgcagttact accttgcttt cacagagaat agtggttgcg gtgatgcagg
    33121 atcccgattt tctcgatcca cacagggcgg cggcttcggg aacctacgaa atgatgttta
    33181 acgtgttcga gggtcttctc aagcccgacc ctaatggtac tgtaatacct gccatagccg
    33241 aaagctacag tatttcggaa gacggtatgg tctacacttt caatctcagg agcggagtga
    33301 agtttcatga cggatcagag gttacggtag acgatgtttt gtactctttg aaccggctca
    33361 aaggctctaa cggtgaaaga ggactttctc ctgattttga gaagtttgtt tcgaagattg
    33421 aagcggcaga tgacaagact gtcagggtca aactcagtac actcaacact gactttctcg
    33481 agaagttcat agtggcaatt atcccggaaa gtaacgaaaa ccatgaaaag aatcctgtgg
    33541 gaacaggtcc ttttaggttc gtagaatata gaccgggtca gaggctagtt atcgaaaagt
    33601 ttgaggaata ttggaatcct gagctgccaa aagtcgagga agttgaattt agaataattc
    33661 ccgacaatca ggcagcaata atgagtttcc tggcaggaga ggttcagata cttccgagac
    33721 tcgatgctat tcaggcaatg gcactaggag atcgatacaa tctattaact gccgaacaga
    33781 atatggttca gttgatggca atgaaccatg cggtcagccc tttcgacgac attagagtca
    33841 ggagagcgat aaaccatgct gtggacaagg aggagattat agaaatcgtg gccggcggtt
    33901 acgggacaaa gctcggctca aacatgtcgc cgataatgag cgcgtattac agagaaggtc
    33961 ttgaggatta ctacgaaccg agtgtcgaga aagcaaaagc tcttctctca gaagctggat
    34021 atccagatgg atttgacaca aagattactg tgccttccaa ctatcagttt catatagata
    34081 ctgcccaggt aattgcggaa cagcttaaga gagtgggtat aagggcggag atcgagcttg
    34141 tcgagtggag tgtttggctg gacagagtct acacagcaag agagtacgaa atgacaataa
    34201 tcggtcttac tggcaaattg agtgcttacg aaatattgag gcgatatgtt tccgactatc
    34261 cgagaaattt ctacaactat tccgacagtg aatacgaccg tataattagt ctggctattg
    34321 aaaagactgc cgtcgaagaa aaagcatcta tgttcaaaag agctcaagag attcttacag
    34381 aagatgccgc tgcggtttac atcatggatc ccaactttat cgttgctcta gctcccaatt
    34441 tgcacggata cacggtctat cctctctacg tgcaagacat gtcgactctc tattatcgct
    34501 gacaaggaga ggcatttgtg tttgtttatt taaagaagct ctttgccatg attctaacgg
    34561 tgcttctggt tttgctgata acctttgtta catttgagat cattccgggc aatcctgcgt
    34621 tgtcaagact tggtgtggat gctcaagaat cccagtatga agccttgtca gcagaactag
    34681 gtttggatga tccattaggg ataaggctgt ccaggtggtt gaaagggctt ttcacgggtg
    34741 atcttggaat ctcaatgaga tactccgttc ctgtatcgga attaatcctc gacagattgc
    34801 ccgtaactat ttctctcgca gtgatggctt tggccctgat tatcctaata ggcattcctc
    34861 tcggcatagt tagcgcaaaa tatggtgaaa ggctaccagg agttatgatc acagtaatct
    34921 cacagattgg gatggcaatt ccatctttct ggagtggaat tatcttgatc tatatatttg
    34981 gaattctcct tcgctgggta tcacctggag gatatgttcc ctggagcgag gatgcttatg
    35041 gagcgttcaa gtcacttatt ctaccggcca tcgccatagc gcttccatcg attgcatcag
    35101 tcataagata cacaagaaac tccgtaatgg atcagatgaa gaacgattat gtacggctcg
    35161 caatgtcgaa agggatgaac ttgaattcag tgttattgag acatgtactc aaaaatgccc
    35221 tgattcctat tatcactgta cttggaatga taacggcaaa cattttaggc ggatcaatcg
    35281 ttatagaaca ggttttcacg cttcccggag tcggaagact tctgataaac gccatcacga
    35341 ccagggatct ccctctggtg cagggaatgg ttttgtacat tgctttcata gtcgttatta
    35401 ttaacttctt tgtcgacatc atatatacag ttatcgatcc aagaatcaga ttgggtcaga
    35461 gggactaaca tgaacaaaag gaggatcaat ctatggattg ggacagtgct gatttccatt
    35521 ctgatgttga tgatggtagt cagtctattc tatactccct ttcctgtaac ggagatcaat
    35581 agacttgaac gatttgaacc tccttcagcg cggcacctcc tgggcacaga caactttgga
    35641 agagatgtgc tcagcagaat aatgaaagga tcccagacgg ccttctttgt gggactagtc
    35701 gcggtatcga ttggcagtgt ttttggcgta cttatcggcg caatctctgg ttactttgga
    35761 ggaatagtcg acgagataat aatgagaatt gtcgacgcta tgatggcttt cccaagcatc
    35821 cttcttgcac tgatgtttgt atccgttttt ggagtaggct tgagaaatac gattattgca
    35881 atagggataa tgtcaattcc ttcgtttgcc agaatcacaa gaagcggttt cgttcaacac
    35941 aaagagctgg attacgtcaa gaatgcaaag ataaagggca tctcgaattt gcggataatg
    36001 ttcagacaca tactgccaaa tgtgctttcc ccaatagtcg ttgctgcttc aatgggattt
    36061 gcaaacgcag tcctcgccga agcggcgctc agttatcttg gacttggaat tcaaccgccc
    36121 aatcccagct ggggaagaat gttgagtgag tctcaggtct tcataaccgt cagcccctgg
    36181 tacgcgatag ctcccggaat ctttatcacg cttcttgtgc tgggattcaa cttcctgggg
    36241 gatgggatca gagacattag agagaatagg tgaaagagaa tgaactgtga aaacgagccc
    36301 ttactagaaa tcaaagatct caaaattgga ttcaagactc cagaagggcc tcttcacgct
    36361 gtgaaaggaa tctctcttga agtaaaaaag agagaaatcg ttgggatagt cggagaatca
    36421 ggctgtggaa agacagtaac tgcattgtca ataatgcgcc ttttacctga taattcattc
    36481 gctactggaa gcataaattt ctccggcatc gatcttcttt cactcacgga agacgaagcg
    36541 aggggaacac gcggaagaga tatatcaatg attttccagg actccctctc ctctctaaat
    36601 ccccttatga aagtaggaag acaaatcgac gagaatctca tgatgcacac ttctctcgac
    36661 aaagcagagc gattctcaag ggttcttgag atcatgaaag gtatcggtct tccagatcca
    36721 gaaagaacat acggcaagta ccctcacgaa ctctccggtg ggatgagaca gagaatagcc
    36781 attgctattg ccatcgtgtg taatcccgat ctgattatcg cagatgaacc gactaccgct
    36841 ctggacgtca cagttcaggc acagatactg gaacttctga gaaagataaa ccgtgaaagc
    36901 aaccgttcta ttatttttat ctcgcacaat cttggagtca ttcgggaaat atgttctaag
    36961 gtctatgtaa tgtatgcagg tcacatcgtt gagagagctt ctgcagaggc acttttcacg
    37021 aatccaggcc atccttatac tttgggcttg ataaaagctc taccaaaact tgaatctaag
    37081 gggaaagagc ttttcgatat tagaggcaga gttccatcca ttgccgattt aactcaaggt
    37141 tgtccttttg aaccaagatg tgattactca ttcgagagat gcaggaagga gttccccgat
    37201 ctgaaaaaaa tcgattccga tcacgaggtg cgatgttttt tagccggagg caaactttga
    37261 aggagagaat tctcgagatt caggatctca cgaaacattt tcgcttgaga agaaccagct
    37321 tgttacggag gccgggctct ataaaagcgc ttaatggaat ttccttcagt atctctagag
    37381 gagagtcctt cggcatcgtc ggtgaatcgg gttgcggtaa atcaacggca ggatatagca
    37441 tagtgggact tctgaagccc acggcaggga aggtattgtt caaaggcgaa gaagtcaatt
    37501 caatggatgc aaacagtctt cgagagttca gaaagaatgt acagcttgtt tttcaggaca
    37561 cttcaggctc tcttaatccc agaggaaccg ttgaatggat actgtctgaa ccactaagag
    37621 caatgggtat ggcgaaaaaa caaatcagaa atctagttgt tgatagtctt gaactcgtag
    37681 gattaggtaa agatctccta ggcagatttc ctcatgagct ttccggaggt cagaaacagc
    37741 gtataggtat cttgaacgcg ctcatactcg atccggaagt catcatcgcc gacgaaccgg
    37801 tgtcttctct agacgtctcc attcaggcac aaattcttaa ccttatgaac gaccttcagg
    37861 ataggctttc tctaacttat ctcttcattt cgcatgacct gaacgtcgtg cactatttct
    37921 gcgacagaat agccgttatg tacctcggag aaatcgttga gctttccgaa tccgaagaat
    37981 tgtacagatc gcccctccat ccctatacac agtctcttct ttcggcaata cccggcggct
    38041 cttatcggaa gactgaaaga ataattctcg aaggtgatgc tccaagtcct atgaatccgc
    38101 catctgggtg tttttttcat ccccgatgtt tcaaaagaat ggagatttgc tcgagagtca
    38161 aacccaaaat aattgaggtt tcgaaagatc atttcgtaag ctgtcacctt tactgagcat
    38221 tccactctct tgatattgga acccgaggcg gttttttgac cggcatcgct tcaaagagca
    38281 cgaatcaact ctttcatccg atcttcttga gtagatacat actaagcaca taaagacagc
    38341 ttgccataac agccaatgag ataaaagcaa ctccaaacga gactgtgtca gcaattcttc
    38401 cgaagatggg gaagaaaact atcattacta agctgtacat catgcttgca aaggatatta
    38461 tcgttgccct gctcttggat ggaatcagct tgtttatgta gtcgttaccc gctacaaaca
    38521 acatagcttc aatcatggtt acaaccatga agaagggtaa cttgtttgaa caaagagcca
    38581 caccccacat ggatgcgacg gagattagag gcagtatgat taggactttc ttctccttca
    38641 gcagatgaga tagcctctga accttcatgg ccactaatcc ggctagaaac gaaccggccg
    38701 cgaggtatat gccaatctgg aactcggtac ggccaagtcc tttccagtag ttctgaatgt
    38761 a
//
LOCUS       DAOQYI010000018        32564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1595_length_32564_cov_72.620351, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000018 DAOQYI010000000
VERSION     DAOQYI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 32564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32564
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1595_length_32564_cov_72.620351"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..701)
                     /locus_tag="PKL93_06390"
     CDS             complement(288..701)
                     /locus_tag="PKL93_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNQ70927.1"
                     /translation="MIYKENAKKILETFKTVVVVGFSKSPEKAANYVPIYLIEMGYRI
                     VPVNPSMEEYSGLKVYPDLEAVVADGIELEVVEIFRPSEEAESIALKAIELGAKAVWL
                     QKGIYSEKAQLAADENGVVYVEDLCMFEEHRRISL"
     gene            complement(698..2197)
                     /gene="lnt"
                     /locus_tag="PKL93_06395"
     CDS             complement(698..2197)
                     /gene="lnt"
                     /locus_tag="PKL93_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNQ70928.1"
                     /translation="MVLVLVSAALTALAMPGMLWGYLIWVALIPLFMAMKDVSPLKGA
                     LRAFVWGFVYLLLTHYWQLPVLTVNVPEVLNSFPNFIGVVVFFLMGIVMAVPFLVFGL
                     VYGLYQRFFDRYPVILALFAASFFTVTEYLREIGPLGFTNGRLSDALLNGQQGISQLL
                     TIGGPLLLVFIIVFVNHCLSYLFVNRTRDRAILIVVSVAIVALVNAAITALVPIPHSS
                     SKYESTLYALQTNISMQMKYYQPPDETLAVVSRALREIPEGSVVILPEATFMSDIRRN
                     STGTQLQQIAQERNLKILIGFPTYNDHNYNQVRFVTAEGFSDKYYAKIQLTPFVESLP
                     WPKVFGVFSFLKFLDYFDAGEEFTIFSVDEHRIGAQICFDSLYSNVARGLTLNGANVI
                     VTATNDGWFDISTALNQHLSKSIARAIENRRYVIQVSNTGISAIIDPYGRIVKRLPTS
                     KELNADYVIGEFQYLPTVKTTLYTRFGNWFFWFSLFLGIALIILGGVVL"
     gene            complement(2197..3039)
                     /locus_tag="PKL93_06400"
     CDS             complement(2197..3039)
                     /locus_tag="PKL93_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNQ70929.1"
                     /translation="MIRLIVIDLDGTLLNDEKKISQENENAIRRALEMGIAVSISTGR
                     SYVSGHEYVEQLGIDVPVSYQNGALVTFGHGEEIKVISRSVLDPSKARMIYKRATEKS
                     LNTLVFFDFFTLPDISTVVPYDSPYREYYRHNKYRMVFEENPLKRISNDGIPEIAVEG
                     KESLIKDLILESNSHLQNVTVVKNDSLEDHAFYEFFGPGVGKEKGLRHILDYLNLDWD
                     SVAHIGDNYNDLEILKKVALPIVMANGPLGVKKHASFVTVRDNNSGGVAEAIERILRT
                     RGEL"
     gene            complement(3017..4000)
                     /locus_tag="PKL93_06405"
     CDS             complement(3017..4000)
                     /locus_tag="PKL93_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family protein"
                     /protein_id="HNQ70930.1"
                     /translation="MTEQIENSINNTIDEIRELAGGERLTVAFSGGLDSTLVAYLASK
                     ALGRDRVKLINVCFGPYSYSRGLEIVASLAERLDLRLEFTPGYEAQENVWKRGPSCNR
                     CTRLAKLPAVRSGVLGLVATGANQSDTWGKTGIVIKDGFYAPLRNWTKAEIEEALSYL
                     NIEVPKIGEAPVREGCKLKHLLKIMAKPSYHGYSVAISNEILLDELDGFSHTLANVKI
                     IGPLSRNVALINVSPLPSERKRGRIAASLESLDSIDEVRWVEGPMVLKIIANPGLFNS
                     PEAREWVLNGRLAPEFAFPIHARWVKSKNNRLETFQVIDCWRLEDDSAHSY"
     gene            complement(4058..6247)
                     /gene="oraE"
                     /locus_tag="PKL93_06410"
     CDS             complement(4058..6247)
                     /gene="oraE"
                     /locus_tag="PKL93_06410"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HNQ70931.1"
                     /translation="MLPVNEKLRVEEILKDLEHYVPKRKGWTWRKKLPEGTKVDGFYY
                     DEISEPLKNSVGLPASHYFDNIDPQPDPVITSEIASGRFEDDVRRMRMAAWHGADHIM
                     VIRTLGQSHIDGLIEGTPEGIGGIPITRKQLRATRKALDIIEEEVGRPINFHSYVSGV
                     AGPEIAVLFAEEGVNGAHQDPQYNILYRGINPLRSFVDAAVAKKIMASVDMLQIDGAH
                     NANASAKRAWKVMPELLVQHAINCVFSVKAGMKKDLISLSTVPPVVSPAPEFKLNFIY
                     ALTVRELFKEYRFRAQMNTRYIESDLVDATRIHVLDTLISRLTKADIQSTITPDEGRN
                     VPWHVNSIRGVETAKHTLVALDGIKDLLKVNEEVVRPSIRELKMRAILMMEEILEVGG
                     YFEAAEKGFFVDNGLYPERNGDGIARPKDGGIGAGTVIPRDADYFAPVCEHFGYNRIP
                     EGLSSPDEPIGGCTLHDRAKIKYIDELDESDNVNLRITQQLEDREKGLISPEVEWWGD
                     GWVQLDMTIPDDPEHSEAAAIEIAKRMGFTDASVISKTILHPVEGTYLELKARVPFKI
                     ERDSLQLPQKPDLLSEEEITAFIDEHPMRVVAGTVGNDEHSVGIREILDIKHGGIEKF
                     GFKYTYLGTSIPPEKFIDAAIETGADAILVSTIITHNEVHVDNMTKIAQLAVEKGVRD
                     KVIIISGGTQITNDLAVSCGMDAGFGRGTKGINVASFLVKKKRDLLR"
     gene            complement(6234..6620)
                     /locus_tag="PKL93_06415"
     CDS             complement(6234..6620)
                     /locus_tag="PKL93_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HNQ70932.1"
                     /translation="MMSIQRSDDFEKRSVHLKNLTDEELDRRFWELADKIVEPLIDLA
                     KANTSPSIERSVLLRMGLDSSQAGSIVTKAVEHGLLGKGAGNIVLTYAKMNSLTLEKA
                     VEELAVGKGWEALVSNFRGGDRNAAR"
     gene            complement(6598..8022)
                     /gene="ortB"
                     /locus_tag="PKL93_06420"
     CDS             complement(6598..8022)
                     /gene="ortB"
                     /locus_tag="PKL93_06420"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HNQ70933.1"
                     /translation="MKDRSYEAVMARRKEIMKATVGVDYDRYELDGVAFDYERLMKDT
                     SYPIASIRRIQSETGVGNTPLIELKNITRLVRSMSKSGYGARIFLKDEATNPSGSFKD
                     RRASISVSRAKELGYKGVIAATSGNYGAAVASQSMKRALKCIVVQECYDSRGKGQPEI
                     LEKARACEAYGAEVAQLTVGPELFYYFLLLLEETGYFNASLYTPYAIAGIESLGYEIV
                     EQSNTLIGRNPDYVLATHAGGGNLTGTARGLIKAGADQTKVIGVSVDLSGLHMASNRD
                     FNRKSFTTGHTGFGIPFAVLPDRSDVPRNAARALRYMDRYLLVTQGEVFYVTEMLARL
                     EGLQRGPAGNTSLTAAIALARELPEDKTIVVQETEYTGAGKLPSAQLTFARDNGIEIV
                     RGDPIKMDSPGKRIAIPESYSQIAYKEVNMDDLRRSYIKQLMKQGVTLIDSDFEFLSE
                     ELKIPVTKVRELVKEVSDDVDSEK"
     gene            complement(8012..8326)
                     /gene="ortA"
                     /locus_tag="PKL93_06425"
     CDS             complement(8012..8326)
                     /gene="ortA"
                     /locus_tag="PKL93_06425"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HNQ70934.1"
                     /translation="MSVIKGQWVQIRRIILSAGERAPSVPEDTKEVPLELRVKGFLLE
                     EKSEVGEMVTVETASGRKVSGRLEAVEPAHKHNFGDYIPELSEAGNELTRWLTGGDND
                     ER"
     gene            complement(8329..9354)
                     /gene="ord"
                     /locus_tag="PKL93_06430"
     CDS             complement(8329..9354)
                     /gene="ord"
                     /locus_tag="PKL93_06430"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HNQ70935.1"
                     /translation="MAYRVLVWGLGAMGSGVARNVSQKEGLRLVGAVEKDPQKIGRDL
                     GEYLGGEKTGRQICSDVEKAISETRPDIVVIATNSFVEEVLTKIETVAKHHVDILTIA
                     EEMAFPFFSHPEESEVLENIAWRYGVSILGTGINPGFVLDFLIIAMTGACLKVDRIEA
                     RRINDLSPFGRTVMKTQGVGTSPEEFKKGIETGEIVGHIGFQQSIAMIGNALGWDIDR
                     IEESREPIISKTERKTSVAHVLPGMVAGCRHIGRGYCGDKLMIELIHPQQILPEKEGI
                     DTGDYIDIFGEPDIHLSIKPEIPGGKGTIALATNMIPAVIESGPGLLEMSELPIPRCL
                     LNEIREI"
     gene            complement(9530..11224)
                     /locus_tag="PKL93_06435"
     CDS             complement(9530..11224)
                     /locus_tag="PKL93_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNQ70936.1"
                     /translation="MRVYKRLGELLIDQGLISEDTLQQAVSLQKKVNKPIGEILVGMG
                     VISWEDIYDSLSKQYGLKVLQDLPNMLSPEVLRMIPKPVADRLNVIPIEYLPENGLLR
                     VVTTDVLKVPQIDRELSFLTGNKVSTLLVPPPMFDALYRSSYEESASSEIIDNTFSID
                     QRSEVDLDEEKVDETDDTPVAKFINSLLDNGIRSDASDIHLEPYEKMAVARMRVDGVL
                     RKILSYPRKAHNSVVSRIKVMSNLDISEKRMPQDGKFYIRRGNEQFDMRVSTMPTIFG
                     EKVVMRILRVSNAKKQLEDLGLSDYNRQRFESIITSPYGIILVSGPTGSGKSTTLVAV
                     LNQVTSDKVNVLTAEDPVEYTIEGISQCQVNTDIGLTFARYLRSFLRQDPDIIMVGEI
                     RDRETAQLAIEASLTGHLVFSTIHTNSAAGAVARLVNMGVDPFLLGTSLIGVMGQRLV
                     RKLCNNCKVKIPVREEVRKIASMLYPNRDDFSEYIPGNGCPECRGMGYRGRTSISEIL
                     VVDSKLRQLIGENASEREIAAAAISGGMRTLYNDGVQKVIDGITSLEEIKRVAIEY"
     gene            complement(11228..12274)
                     /gene="ftsZ"
                     /locus_tag="PKL93_06440"
     CDS             complement(11228..12274)
                     /gene="ftsZ"
                     /locus_tag="PKL93_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNQ70937.1"
                     /translation="MSFEIDTGKKNTQIRLPSIKVIGVGGAGGNAVNRMISEGIHGVT
                     FIAANTDIQVLESNKADLKIQLGTELTRGLGAGGNPNVGERAAEESVDEIGTFLEDTD
                     LLFITAGMGGGTGTGAAPIVASIAREMGILTVAVVTTPFFFEGNTRLKTAHEGLRRLK
                     NSVDTLIRISNNKLLQELPPNTSIVDAFAKADETLHHGIKGISELITKRGYINLDFAD
                     VESVLRNAGTAMLGIGVGSGERRAEEAARRALESRLLEKPIDNATGIILNVSAKNITL
                     REMNIAAAIVRQNCSEDADVKLGLIVDPDMNDDELDITLIAAGLELDEGELMGDASDI
                     PAIYRFGLDLSEEE"
     gene            complement(12264..13556)
                     /gene="ftsA"
                     /locus_tag="PKL93_06445"
     CDS             complement(12264..13556)
                     /gene="ftsA"
                     /locus_tag="PKL93_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNQ70938.1"
                     /translation="MARGKDYTVSLDVGTNTLKGVVVSSEQTGEMTLEAYGSVKTVGL
                     DKGEVKDAVALKQSIQKLIEDLTGQMGKKDVEADFKISFTDGDYSVFSENIEEVLSED
                     KQVMVKEQTIVGLMSRLKAEKMKTGNTNIHRSYIRKYILDDDKVVFNPVEMYAKKLNV
                     EMVFVSSEGKSVEIFRRLFEELFGRGDFFISPALISASEAVLTDTEKQHGVVSVVLGH
                     SFSEMVIYRENLPVYISRIPLGIRHIVLDIARVLGTSVDEAERLLVNYGHCSMFPPDA
                     EDVVEYFGLDERTRKNVSVRRLSTVIYARVKELLNKIRKEIQLFVINNPEYSEERIPG
                     GVVFTGGGSKLRGLTDVGVESLKMPVRIGTYETSFNRRIENSHDVANDPIFSSCLGNL
                     VSPEEIQGEAVESVVERPKKGFASFIRSLFFGGVDDEL"
     gene            complement(13562..14200)
                     /locus_tag="PKL93_06450"
     CDS             complement(13562..14200)
                     /locus_tag="PKL93_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4894 domain-containing protein"
                     /protein_id="HNQ70939.1"
                     /translation="MSLSLRAGICMVILFAMWILCIANFVLESFFTEPVEQLTRIVNK
                     DEILIEFPLAFRRAWKTDIIYPPGSVDVSGGRVYLKPGKYIVSYEGKYYWVSEDFVIV
                     DYAHLSEILSYPIMGGIQFILTDGVYVASERDIDIFAGAVEGILADRRVLALVSYFDF
                     KNNVLLLRRGIRVKVLDWESLNTNKELLLQLENASDRSEYIFLSDGKLLRAR"
     gene            complement(14197..16092)
                     /gene="gyrB"
                     /locus_tag="PKL93_06455"
     CDS             complement(14197..16092)
                     /gene="gyrB"
                     /locus_tag="PKL93_06455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNQ70940.1"
                     /translation="MSDLYNAQNIKILKGLDPVRKRPGMYIGSTGKSGLHHLVYEIVD
                     NSIDEAMGGFCDTINVVITEDGSVIVSDNGRGIPIDIHPDTGTSALEVVMTTLHAGGK
                     FSKDSYKISGGLHGVGASVVNALSEWMIVEVKVDGKIYRQKYERGKALAPVEVVGETN
                     ETGTVTSFKPDPLVFTVTDFDFDILEARFKELAYLNGGIKITFEDRRIGEKRSYHFEG
                     GIVEFVKALTKNKKSIHKDPIYLEGTYNDVKIQLAMQYTSSYDEDILTFVNNIKTIEG
                     GTHLTGFKTVLTKTMNDLGRKHNVLKDKDPNLQGEDLREGLSAILSVFVKEPQFEGQT
                     KAKLGNDEAFEAVMKVVKEKLEEYFDYNQKDLKAILSKALEASRARLAAKKAREMVRR
                     KNALENTTLPGKLADCISENLDETEIFIVEGDSAGGSAKMARSRETQAILPLRGKILN
                     VEKAGLDRMLKSETISNIIVALGTGMGEDFEIKNLRYGKIIIMTDADVDGAHITTLLL
                     TLFYRYMTPLITNGKVYVAQAPLYKIEMNRQKHYFYSDEELTTFLKEHSDRKLNYSRF
                     KGLGEMNPEQLWETTMNPNDRKLVKITIEDAEEADRVFTILMGSEVEERRSFIQRHAM
                     SISNLDV"
     gene            complement(16108..16404)
                     /locus_tag="PKL93_06460"
     CDS             complement(16108..16404)
                     /locus_tag="PKL93_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNQ70941.1"
                     /translation="MKWNHRTIGQIFNDLAKSSSLFRKIRVFELNKDWGEIVGEPIAN
                     HSSIVDFSEGTLIIRVDDGMWLNEMKLREKILLERMNSSLGVEAIKRIRFRIGR"
     gene            complement(16367..17251)
                     /locus_tag="PKL93_06465"
     CDS             complement(16367..17251)
                     /locus_tag="PKL93_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNQ70942.1"
                     /translation="MLNYIADQWCDEQSSLVSVLYPGVMNSESVYEVVRTYNGLPFAF
                     RKHFERLKQSAALMGLEVPFSCKELNSIIIEGLEKNKGFQTQDFRIRISLISDGARSR
                     PAIIFSDLPSPSKDIYELGVKISISPFLKPSADIVNPQLKMPGSSWIIRTRKALGDNY
                     DMLILNEKGNVCEGSFSNVFLVMDGSVVTPDIRSGVLPGITRDNVIGLCESLEISVEK
                     RPVPAWELFCADEIFITHTSVGIVPVRRLEDKVLIEDFTDGMTRLLLDNFEGYIMTED
                     SNWSGLDEVEPSNYRADI"
     gene            complement(17217..18494)
                     /gene="mtaB"
                     /locus_tag="PKL93_06470"
     CDS             complement(17217..18494)
                     /gene="mtaB"
                     /locus_tag="PKL93_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNQ70943.1"
                     /translation="MKQKVSIYTFGCKMNQYESQAMAEMLHDYQVGFSQDKADLFIVN
                     SCTVTSEAERKLRQLFRRLKGLNPNSKIIITGCYSQLSPNELRELGADEVIGVREKKA
                     IHKYVSRQLNRPDGVAPSEFLTVTSSIEGRTRAFLGIEDGCLNCCSYCRVRLARGSKI
                     ISKPIDLVKREFEGLVSRGYREIVLTGINIGYYGFDLDSSLVKLLIELDKLEGEWRIR
                     LGSLDPDRVDGELLHLITTSRRMARHLHLSLQSGSDRILRAMRRKYTVDEYLRAVDRA
                     RSVDTRFAFTTDLIAGFPGENDSDHKESLKVVEQVEFLKVHVFRFSRRPGTEAAEMKD
                     QIDSGTKKARSHELIEAGKRSRKKYLERQIGRKASVLIQKTGHTESSGFDEFYAPHAI
                     EGAHEGFVNTTIISIESQREGSDAELYCRSVVR"
     gene            complement(18491..18943)
                     /locus_tag="PKL93_06475"
     CDS             complement(18491..18943)
                     /locus_tag="PKL93_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ70944.1"
                     /translation="MKVKEAMIRDVSAIFEDETIEDFIVFCLRQNKSGLPVVDEEFKV
                     VGFLSESDVIKSALPSYFSLLQSASFIPDTHQFVIRLGKIKDDHVSQHMVKPPVLIKP
                     DDTVIYAADLLIKNGLKIMPVVDDEGKLVGIVNRIYLIHVATQGKINR"
     gene            complement(18949..19908)
                     /locus_tag="PKL93_06480"
     CDS             complement(18949..19908)
                     /locus_tag="PKL93_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ70945.1"
                     /translation="MDVLTVTLNPALDREIIVENFKINEFHRVKNPSYSVMDPGGKGI
                     NVSVILSGLGVRNVAMGFLGGYIGKVVEERLRLISDLITTAFVHVEEETRENIAIVDP
                     LGETITEINSSGPLIKSDDLRMFIRRFEVALSRAKHVVVSGSIPRGVDNDICKTLCKK
                     VADSGKISFAEGIGPAFEIAVEAGVITVARPDLRSRKLLFGETMVELEDYVRTAKKII
                     ESGSRLAILSYAIEGDVIATRDGIWLFKTKGHIDRSHLLGTGDAFMAGVVHSMIENEN
                     DCFLAAKRGMAAAVAEAEYIGKELISLEDIERHLDSFDIRKLE"
     gene            complement(19932..20648)
                     /locus_tag="PKL93_06485"
     CDS             complement(19932..20648)
                     /locus_tag="PKL93_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ70946.1"
                     /translation="MIETALSLKGFTLLVEGRKMLDGITFDVPKGSIALLQGARGAGK
                     SALLRSFIHLNEELFDRVSYTGSIKLFGEDIDSLERKSVRKRVAYVDTTFLEAMSNFT
                     LIEFFRFLKGKRFEFEDFAESELDLFHELDLLDLLSLKAETSLKTIPLFKRLSLLVFS
                     TLIRHPEIIILDNILDHLDDDACTEVKNVLLDTRNGVTMIISSRFVLRLLDISDLLIV
                     LKNGKISYVGSPEVFVLNNR"
     gene            complement(20899..22149)
                     /gene="rpoD"
                     /locus_tag="PKL93_06490"
     CDS             complement(20899..22149)
                     /gene="rpoD"
                     /locus_tag="PKL93_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNQ70947.1"
                     /translation="MEKQKKTKSGAKVIADNIKSLAEKIADDEADSDETGDNSALMTK
                     EEIDKRIKKLLRQGKKKGFVTYEDIDSAFPPDYEGFDSSLVESIYEEFEKNKINIVEK
                     EPVEDDDDIESESSEELVSILETTPEMYDNISLKDPIKMYLKEIGKISLLTPSREREL
                     ARRAQKGEPKAKEELITANLRLVVSIAKRYIGRGLTFLDLIQEGNIGLIKAVEKFDWK
                     KGYKFSTYATWWIRQAITRAIADQARTIRVPVHMVETINKLNKVIREHLQDHGEYPTI
                     EELGNLTGKTPEKIEEILAASKETVSLESPISGDEESTMGDFIHDDSMEQPEEAAMKM
                     LLREQIDQILDTLSPREAMVLKMRYGLLDGKTKTLEEVGQFFNVTRERIRQIEVKALR
                     KLRHPSRSKQLKALLGMLNTSKRD"
     gene            complement(22151..23860)
                     /gene="dnaG"
                     /locus_tag="PKL93_06495"
     CDS             complement(22151..23860)
                     /gene="dnaG"
                     /locus_tag="PKL93_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNQ70948.1"
                     /translation="MFTKEELDEIKKSINIVDFIGRYVNLQKAGSSYRGLCPFHNDND
                     PSFYVHPQRGFFHCFGCGEKGDVISFYQKIENLSFAEAVKRLADYAGIPIQVDTAESE
                     YDKFTTIMSRLAGIYNRELKEKRPETLAYLLEKRRISQNTIDEFQLGYSPDERTFPQS
                     LPSKLRTDEKTLLQLGILVRRGATYSDRFAGRLMIPIDNESGKVVGFGGRIMDAEKGP
                     KYINSSESKYFQKNRLLFNLSRARAAIKQLNYAVIAEGYFDVISLFEAGITNSVGLLG
                     TALTERHLRILGNYTRNLLFFLDSDEAGQTASLRSIDIAEKLDFGTAVVFSRDQKDPA
                     DLFVAEGPKAIKETLALAIPGPAFRVEFFSRKLDLSIPLGRKHLIEHLKPYVISFRST
                     GNLAAVQSTIAALAQKTGYSERELESALRGGMSKSSGGEMKSGLALKLKDHIRIYLQH
                     PKLRATVVKQLELMNESRDLKELLKGMKKGLELEELLDLVEESIGRELIELASTCIDF
                     DTAQRVLQSTGLYANKRLVEEEMAEIDRRLPKVKDEGARKALLIRRIELRRMLERKMK
                     GGD"
     gene            complement(23925..24329)
                     /gene="rpsI"
                     /locus_tag="PKL93_06500"
     CDS             complement(23925..24329)
                     /gene="rpsI"
                     /locus_tag="PKL93_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNQ70949.1"
                     /translation="MADFVDYYGTGRRKTSVARVHLRPGTGKLLINGKEYKDLKEYLS
                     NDAWVRSALKPAVVTNTIGRFDMIIRVNGGGLGGQAGAISLGIARALVKFNPDLKKLL
                     RQNSLLTRDPREVERKKYGLKKARRAPQFSKR"
     gene            complement(24342..24866)
                     /gene="rplM"
                     /locus_tag="PKL93_06505"
     CDS             complement(24342..24866)
                     /gene="rplM"
                     /locus_tag="PKL93_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNQ70950.1"
                     /translation="MKIQKTPLCRWAVTHSKVQTKRKGEIEKTLGYLVDHNGNKIEKK
                     WYVVDATDVPLGRLAGQIAMVLMGKNKPYYTPHLDTGDFVIVVNADKVKLTGKKLDQK
                     KYYRYSGYPGGIKERTARQMMDKFPDRVVRLAVKRMLPKKALGEKMLKKLKVYAGPEH
                     EHAAQKPELIELVK"
     gene            complement(24894..25139)
                     /gene="rpmA"
                     /locus_tag="PKL93_06510"
     CDS             complement(24894..25139)
                     /gene="rpmA"
                     /locus_tag="PKL93_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNQ70951.1"
                     /translation="MARKSAGRSNGRESNPKYLGIKRGESSAVKAGSIIVRQRGTKIH
                     AGKNVGLGRDHTLFALIDGKVHFEEKNNRKYVSVYSE"
     gene            complement(25141..25464)
                     /gene="rplU"
                     /locus_tag="PKL93_06515"
     CDS             complement(25141..25464)
                     /gene="rplU"
                     /locus_tag="PKL93_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNQ70952.1"
                     /translation="MYAIIEASGRQYRVEEGMTLFTERQNGKESGDEIVFDRVILVHN
                     GEEATVGKPYVNGAKVVGKVVSHGRDKKVLVVKFGPRKNFDRVNGHRQWFTEVSIEKI
                     ETGVK"
     gene            25709..25797
                     /locus_tag="PKL93_06520"
     tRNA            25709..25797
                     /locus_tag="PKL93_06520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25743..25745,aa:Ser,seq:cga)
     gene            25835..27835
                     /locus_tag="PKL93_06525"
     CDS             25835..27835
                     /locus_tag="PKL93_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ70953.1"
                     /translation="MILKEILELLKSKKGKMDYSIPKEWLPDGYSGTLKLRDRYIFVD
                     PYEFGSSIIESIAAKAEPGVDYSKSLGRIRNESDNLWIKSAIVYGSFVRSTAAYSHQD
                     PEFFSDLHSQEYSESGTFLKMIYMLPYLSKMGFDTLYFLPVTSYSDKFKKGELGSPYS
                     VKDFFSIDERYHDVLLEDMDVEREFKAFVEAAHIMGMRIVLDFIPRTSARDSALILKH
                     PEWFYWIDASHLKDYRPPKIDGLGFDQARVETLPVIYSNENVKKHLAMFRYSPEKLNP
                     EKWRNFVAHHEGSDNFLDELVSEFGVITPPGFSDWINDNQPTWDDVTFLRLYKDHPEE
                     SRKFLSEDQPPYVLFDVVKSSFFPGKEPMTDLWETIENIMPFYNSKFGVDGARLDMGH
                     ALPRELELRIIRKAKENDASFAIIAEELVMGNDEKARQSGYDCILGNTWWMEPRITEG
                     KFKELVYEILPRLRVPTLACVETPDTPRAVARPNGKKFSRFATVLNYFLPNGITYVNS
                     GQEIFEIQPMNLGLDNDEDGRYVLPPNDPFYGKLAFFDPYVLHWNADDDMVDLISLLS
                     SLRRDNLELINPHDLKLLWEEDIESAGLFYWNGSKGLLVVANGDFQSMKDFHINLGYF
                     TWKSEHKVAWKLKGFDGHSEWKVGGDFHLSLGPGEVAVAEIK"
     gene            complement(27875..28138)
                     /locus_tag="PKL93_06530"
     CDS             complement(27875..28138)
                     /locus_tag="PKL93_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNQ70954.1"
                     /translation="MEVLKVATHSKPNAVAGALAGVLREKGVAEIQAIGAGAVNQAVK
                     AIAIARGYVAPSGMDLVCVPTFSDVDIDGEVRTAVRFYVQPKK"
     gene            complement(28191..28266)
                     /locus_tag="PKL93_06535"
     tRNA            complement(28191..28266)
                     /locus_tag="PKL93_06535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28231..28233),aa:Arg,seq:tcg)
     gene            complement(28284..28359)
                     /locus_tag="PKL93_06540"
     tRNA            complement(28284..28359)
                     /locus_tag="PKL93_06540"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28324..28326),aa:His,seq:gtg)
     gene            complement(28373..28448)
                     /locus_tag="PKL93_06545"
     tRNA            complement(28373..28448)
                     /locus_tag="PKL93_06545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28413..28415),aa:Arg,seq:tct)
     gene            complement(28449..28524)
                     /locus_tag="PKL93_06550"
     tRNA            complement(28449..28524)
                     /locus_tag="PKL93_06550"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28489..28491),aa:Gly,seq:tcc)
     gene            complement(28537..28612)
                     /locus_tag="PKL93_06555"
     tRNA            complement(28537..28612)
                     /locus_tag="PKL93_06555"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28577..28579),aa:Pro,seq:tgg)
     gene            28832..29746
                     /locus_tag="PKL93_06560"
     CDS             28832..29746
                     /locus_tag="PKL93_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70955.1"
                     /translation="MNGIVTSNLVKRYGSFEALKGVSLSIEDGDLYCLLGPNGAGKST
                     LIRILTGLMKPTSGEVNIAGLDMKRDLKKIREKVGLVSERVILYDRLTPVENLLFFSS
                     MLRIEKRTALKRINSLLEKVDMIEWKDKPVRVFSTGMKQRVNFVRALLHEPSILFMDE
                     PTLGLDPHSTRTIRNMVRELNESGRTIVLTTHIMSEAENIAKSVGIMNKGKLITTGKL
                     EAIRASLNRDRLTITVKNRPEVPFESIDGYMSDSRLNGVVRIDFRPGTTMESVLPKIL
                     QMKLEVIDVDHIKPSLEDVFVELTGEKS"
     gene            complement(29741..30973)
                     /locus_tag="PKL93_06565"
     CDS             complement(29741..30973)
                     /locus_tag="PKL93_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ70956.1"
                     /translation="MIDLIMIGAGNRGYFAYGEAVLSMGDARVVAVAEPDEFRRERFA
                     KDHSIIPQNAVADWKSLLSRPRFADGVIVASPEAVHVEPAIEALRSGYAVLLEKPISA
                     DLSGIRDLISSGADTSRLFLAHVLRYSGFFKTIKSLLESEAIGKLIAVEYTEQVGYYH
                     FAHSYVRGNWRRSELAGPSILSKSCHDMDILTWLLSSRALSVVSHGGLKYFRRENAPE
                     SSTGRCLDCPLKGTCPYSAVTQYLSDNTDWPVNTIGNDMSLEKRRDVLRTGNYGKCVF
                     RSDNNVVDYQNVLVSFENGVEASFSMNAFTSSKTRKIFISGSDGEIYGDFESDRVELR
                     LFSGETKIYKISHDESRHGGGDMEITRDFVRYLKGETAGLSTSFKESIQSHLMCWAAE
                     KSRLEGGLKIDPWSLLAL"
     gene            31089..31310
                     /locus_tag="PKL93_06570"
     CDS             31089..31310
                     /locus_tag="PKL93_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HNQ70957.1"
                     /translation="MTSLTDIKRNDTEIVRVSKREYKGHEFIDLRIYYQDDEGDYKPT
                     KKGITINPKLVDELIDALKKEKDSAPEKE"
     gene            complement(31659..31784)
                     /locus_tag="PKL93_06575"
     CDS             complement(31659..31784)
                     /locus_tag="PKL93_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70958.1"
                     /translation="MTDNAVFIGIQRVLVPKWIAALSRDRFMILDEGQFLEDGGM"
BASE COUNT         7950 a         8360 c         7035 g         9219 t
ORIGIN      
        1 tttaaaatct tacccgcgaa gcggtcattg cgaccgccga tgattttcgg cgccttgcgt
       61 ttagtgtttt atggtagcat gacttccggc gatgttattc gccgcatcac ttcagctctc
      121 gccagtctgc tgatttcgga ggacggcgta ccgtcggcgg ttgacctgat aaggctttct
      181 gcaaagcagc ctaactttct aaggagtcga ggataatctg cttcagcaaa aagtgtaatt
      241 tgtgctcttt tcgatcgcct acccatagct gcgaacacga tacgttttca aagagatatt
      301 cttctatgtt cttcaaacat gcagaggtcc tctacgtaaa cgacgccgtt ttcgtctgca
      361 gctaactgag ctttttcact ataaatgcct ttttgaagcc agactgcttt ggcgcctagt
      421 tctattgcct tcaaggcgat ggactcggcc tcttcagagg gtctgaagat ttcaaccact
      481 tcaagttcta tcccatctgc cacaacggct tcgaggtccg ggtagacttt taggccgctg
      541 tactcttcca tacttgggtt gacaggtact atccgatatc ccatttcaat tagatagatc
      601 gggacataat tggcggcctt ttcgggactc ttcgagaaac caacgacaac tactgtcttg
      661 aaagtctcta ggatcttctt tgcgttctct ttgtagatca aagaacaaca cctccaagta
      721 ttatcaatgc tattcccaga aatagtgaga accagaagaa ccagtttcca aacctggtgt
      781 aaagtgtagt tttgaccgtg ggaagatact ggaattcgcc aataacataa tctgcattca
      841 actccttaga ggtcggcaga cgcttgacga ttcgcccata ggggtcgatt attgcagata
      901 ttcctgtgtt ggatacctga atgacgtatc ttctattttc gatcgctctt gcaatacttt
      961 ttgagagatg ctgattcaga gcagttgaaa tatcgaacca gccatcattt gtcgccgtca
     1021 cgatcacatt tgcgccgttc agagtaagcc ctctggcgac gttactgtat agcgaatcaa
     1081 agcagatttg agctccaatg cgatgttcgt cgaccgagaa gatcgtaaac tcttctccgg
     1141 catcaaaata atcaaggaat tttaggaaac tgaatacacc aaatactttg ggccaaggaa
     1201 gagactctac gaacggggta agctggatct ttgcatagta tttatcggag aagccctctg
     1261 cagtaacaaa tctcacctga ttgtagttgt gatcgttgta ggtggggaaa ccgatcagaa
     1321 tcttcagatt tctttcctga gcaatctgct gcagctgggt tccggttgag tttcttctta
     1381 tgtcgctcat gaaggttgcc tccggcaaaa tcacgacgga gccctccgga atctctctga
     1441 gtgctcgtga tacgactgcc agagtttcat ccggtggctg gtagtacttc atctgcatcg
     1501 atatgtttgt ctgcagagca tagagagtgc tttcatattt gcttgaggaa tgggggatcg
     1561 gaactaaggc cgtgatagcc gcattaacta gggctacgat tgccactgaa acgacaataa
     1621 gaattgctct gtctcttgtt ctgttcacga agagatagga aagacaatga tttacgaaaa
     1681 caataatgaa tactagaagg agaggcccac caatagtaag taactgagag attccttgct
     1741 gcccatttag aagcgcatca gagagtcttc cgttagtgaa gccgagcggg ccgatctctc
     1801 tcagatattc tgtaaccgta aagaatgaag cggcaaaaag agctagaata accgggtatc
     1861 tgtcgaagaa tctctgatac aaaccataaa ctagaccaaa tactagaaag ggaacggcca
     1921 ttacaattcc catcaagaag aagacgacga cccctatgaa attgggaaat gaattcagta
     1981 cttcgggaac gttcactgtc aacactggga gctgccagta atgggttaga agaaggtata
     2041 caaagcccca gacgaaggct ctcaaagccc cctttagggg tgatacatct ttcattgcca
     2101 tgaataaagg gatcagggcg acccatatca agtaccccca aagcattccc ggcatggcca
     2161 gtgcagtgag agctgccgaa acaagaacta ggaccactaa agttcacccc tggttcggag
     2221 gattctttca attgcttctg ccacacctcc gctgttgttg tctctaactg tgacaaaact
     2281 cgcgtgtttt ttgacaccaa gtggaccatt agccattacg atcggcaaag ccactttctt
     2341 caatatctcc aaatcgttgt agttatcacc tatgtgagct acactatccc agtctagatt
     2401 gagataatcg agtatgtgcc taagaccttt ctcttttcca acgcccggtc caaagaactc
     2461 gtagaatgcg tgatcttcaa gggaatcgtt cttcactaca gtgacgtttt ggagatggga
     2521 attcgactca agaataaggt ccttgatcaa ggattctttg ccttctacag caatttctgg
     2581 aattccgtcg tttgagattc tctttagcgg attctcttca aataccatgc ggtacttgtt
     2641 atgtctgtag tactccctgt aaggggaatc atatggcaca acggtagaga tatcgggaag
     2701 tgtgaagaag tcaaagaaga ccagagtatt caggctcttt tctgttgctc ttttgtatat
     2761 catccttgcc ttgctgggat cgaggacaga ccttgaaatc accttgatct cttctccatg
     2821 gccgaaagtt accagagcgc cgttctgata cgagacaggg acatcgattc caagctgctc
     2881 aacgtactca tggcctgaaa cgtagcttct tcctgtggat attgaaaccg caattcccat
     2941 ttcgagagcg cggcgtatag cattttcatt ttcctgagag attttcttct catcatttag
     3001 aagagtaccg tcgagatcaa taactatgag ccgaatcatc ttccaacctc cagcaatcta
     3061 ttacctgaaa agtctcgagc ctgttgtttt ttgacttcac ccacctggca tggatcggaa
     3121 atgcaaactc cggcgccagc cttccgttaa ggacccattc tctcgcttcg ggactgttga
     3181 acaatcccgg attggcaatg atcttgagca ccatgggacc ctctacccat ctaacttcat
     3241 cgatcgaatc aagcgactcc agagaagccg cgatcctgcc tctctttctt tccgatggaa
     3301 ggggactgac atttatcagt gcaacgttcc tagagagcgg accgattatc tttacgtttg
     3361 ccagagtatg tgagaaacca tctaattcat ccagcaaaat ctcatttgaa atcgcaaccg
     3421 aatatccgtg ataggaaggt ttggccataa ttttcaacag gtgtttcaac ttgcagcctt
     3481 ccctcacggg tgcctcgcca atcttcggga cctcgatatt aagataagaa agcgcttctt
     3541 ctatctcagc cttggtccaa tttctcaaag gcgcgtaaaa accatccttg atcacaattc
     3601 cagtctttcc ccaggtgtcc gactggttgg ccccggtcgc aaccaggcca agaactccag
     3661 atctgacagc cggcagcttg gccaatctgg tacatctatt gcatgaaggg ccacgcttcc
     3721 aaacgttctc ttgagcttca tagcccggtg taaactcaag tcttaggtcc agcctctctg
     3781 ccagggatgc aacaatttca agtcctctac tgtatgagta agggccgaaa caaacgttta
     3841 tcaatttgac tcgatcacgt ccaagggctt tcgaagccaa gtaggcaacc aaagttgaat
     3901 caaggcctcc cgaaaaagcc accgtgagtc gttccccgcc ggccagttct ctgatctcat
     3961 ctatcgtgtt gtttatggaa ttctctatct gttcagtcat cttttcaagt ataacgcaaa
     4021 gaaggcggcg aatgccgcct tcgatgttaa ctcgattcta tcggagaagg tcgcgtttct
     4081 tctttacaag gaaagatgct acgttaatgc ccttcgttcc gcggccaaag ccagcgtcca
     4141 ttccgcagga aactgcaagg tcattcgtta tttgtgttcc tcctgaaata attattacct
     4201 tgtcccttac acctttttca acagctagtt gcgcaatctt cgtcatattg tctacatgta
     4261 cttcgttgtg agttattatc gtcgaaacca gaatcgcatc tgctccagtt tcgatagcag
     4321 catctataaa cttctccgga ggaatgctcg ttcccaagta tgtgtatttg aagccgaact
     4381 tttcgattcc cccgtgtttg atgtcgagaa tctcccttat acctacagaa tgctcatcgt
     4441 ttccaacggt ccccgccacg actctcatgg gatgctcatc aatgaaggcg gtaatctcct
     4501 cttcacttaa tagatcgggc ttttgcggca gctgcaagga atctctctcg atcttgaatg
     4561 ggactctcgc cttgagttcc agatatgtgc cttctacggg gtgtaggatc gtcttgctta
     4621 tgacggaggc atctgtgaag cccatccttt ttgcgatttc aattgcggcc gcctctgagt
     4681 gttcgggatc atctggaatg gtcatgtcga gttgaaccca tccgtcaccc caccattcca
     4741 cttcaggcga tatcaggccc ttttccctgt cctcgagctg ctgtgtgatc ctgagattca
     4801 cattgtctga ttcatctagt tcgtctatat acttaatttt cgccctgtcg tggagagtgc
     4861 agccgccaat aggctcgtct ggagaggata agccttcggg aattctgttg tagccgaagt
     4921 gttcacagac aggagcgaag taatcggcat cccttgggat tacggtgcct gcgcctatac
     4981 ctccgtcttt tggtcttgct atcccatctc catttcgttc cgggtaaaga ccattatcga
     5041 cgaagaagcc cttctccgca gcttcgaagt atccgccgac ttccagtatc tcttccatca
     5101 taagaatcgc ccgcatcttc agctctcgaa tgcttggcct tacaacttct tcatttacct
     5161 tgagcaaatc cttgattcca tccagggcaa caagtgtatg ttttgctgtt tctacccctc
     5221 tgatcgaatt aacatgccag ggaacatttc tgccttcgtc gggagttatc gtcgactgga
     5281 tgtcggcttt ggtcaatctt gatattaggg tatctagaac atgtattctg gttgcgtcga
     5341 cgagatcgga ttcgatgtat ctcgtgttca tctgcgctct gaacctgtac tctttgaata
     5401 attcgcgaac agttagcgca tatatgaagt tcagcttgaa ctcaggagcc ggagaaacga
     5461 cgggtggaac tgtggagagg gatatcaaat cctttttcat tccggccttg acagagaaca
     5521 cacagttaat cgcgtgctgg acgaggagtt caggcataac cttccatgct cttttagcag
     5581 aggcgttagc gttatgggcg ccgtcgatct gcagcatgtc gacgcttgcc ataatcttct
     5641 tcgccacagc cgcatcgaca aaggagcgaa gtggatttat tcccctgtat agaatgttgt
     5701 actgaggatc ctggtgtgct ccgtttactc cctcctctgc gaagagaacg gcaatttccg
     5761 gaccggccac tccgcttaca tatgaatgga agtttatagg tcttccaact tcctcctcaa
     5821 taatgtcaag agccttacgt gtggctctaa gctgctttct cgttattgga ataccaccga
     5881 tcccctcggg ggttccctct attaggccgt ctatgtgact ctggccgaga gtccttatta
     5941 ccattatatg atcggcccca tgccatgcgg ccattctcat tctccttaca tcgtcttcga
     6001 atcttccaga agctatctcc gaggttataa cgggatcggg ctgaggatct atgttatcaa
     6061 agtagtgaga agcgggaaga cccacgctgt tcttcagagg ttcggagatc tcgtcgtagt
     6121 agaagccatc gaccttcgtg ccttcgggaa gtttctttct ccaggtccag ccctttctct
     6181 tgggtacata gtgttcgaga tcctttagaa tctcttccac tctcagcttt tcattaacgg
     6241 gcagcattac gatcgcctcc cctgaagtta gaaacgagag cttcccatcc ctttccgaca
     6301 gctagttctt caaccgcctt ttccagggtc agtgaattca tcttcgcgta ggttagaacg
     6361 atatttccgg cgcccttccc gagcaaaccg tgttcgacgg ccttcgtgac tattgatcca
     6421 gcctgcgaac tatcaagccc cattctaaga agcacagagc gttcgattga agggcttgta
     6481 ttagccttcg caagatctat caagggttca acaatcttat cagctagctc ccagaatctt
     6541 ctgtcgagct cctcgtcggt tagattctta agatgcacac tccgcttctc gaaatcatca
     6601 cttctctgaa tcgacatcat cacttacctc cttaaccagt tcccgaacct ttgtcacagg
     6661 tatcttcagc tcttcagaga gaaactcgaa atcgctatcg atcagcgtta ctccctgttt
     6721 catgagttgc ttgatataag accttctgag atcgtccata ttcacttctt tgtaagcaat
     6781 ctgcgagtag ctttcgggaa ttgcgattct cttgccaggt gaatccatct tgatcgggtc
     6841 tcccctgact atctctattc cattgtctct cgcgaatgtg agctgtgcgc tcgggagctt
     6901 cccggcgcct gtatactcgg tctcctggac cactatggtc ttgtcttcgg gcaattctct
     6961 tgcgagagct attgccgcag tcagactggt gttgccagcc ggtccccgtt gtaatccctc
     7021 cagcctggcc agcatttcgg taacgtagaa gacctctcct tgagtgacca gaagatatct
     7081 atccatatat ctcagtgctc gtgcagcgtt tcggggaaca tcggatctat cggggagaac
     7141 ggcaaagggt atgccgaaac cggtatggcc cgttgtgaaa gacttcctgt tgaaatccct
     7201 gttgctagcc atgtgaaggc ccgaaagatc gacgctaaca ccgataacct ttgtttgatc
     7261 tgcaccggct ttgataaggc ctctggccgt cccggtcaaa ttcccaccgc ccgcatgcgt
     7321 cgcgagaacg tagtcgggat ttcttccgat aagtgtattt gactgttcta ctatctcgta
     7381 tccgagagat tctatgcccg caattgcgta tggtgtgtac agtgaagcgt tgaaatagcc
     7441 ggtctcttca agaagcaaaa gaaagtaata gaagagctcc gggccaacag tcaattgagc
     7501 cacttccgct ccgtaagctt cacaggcacg agccttctca agtatctcag gctgaccctt
     7561 tcccctgctg tcgtaacact cctgaacaac gatgcacttc agggctctct tcatcgactg
     7621 agatgcgacg gctgcgccat agttgccgct cgttgcagcg atgactcctt tatatccaag
     7681 ctccttggcc cgtgaaacac ttatggaggc ccgtcggtct ttaaaactgc ctgaaggatt
     7741 cgtcgcttca tccttgagaa agatccttgc gccatagccg gacttgctca tcgacctgac
     7801 caatcttgtg atgtttttca gctctatcag gggagtgttt cccactccgg tttccgactg
     7861 aatccttctg atggaagcga ttggatacga tgtatccttc attaacctct cgtaatcaaa
     7921 ggcgactcca tccagctcgt atctgtcgta atcgactccc acagttgcct tcattatctc
     7981 ttttcttctc gccataacgg cttcgtaaga tctatctttc atcgttatcg ccaccggtaa
     8041 gccatcttgt caattcgttt cccgcttcgg acagttctgg aatgtaatca ccgaagttat
     8101 gtttgtgagc cggctcaaca gcctcgagcc ttcccgacac ttttctccct gaagccgttt
     8161 ctactgttac catctcacct acttcggact tttcctccaa gagaaagcct ttaaccctta
     8221 gttcgagagg gacttctttc gtgtcctcgg gaaccgaggg cgctctttca cccgcagaaa
     8281 gtattattcg acgaatctgt acccactgtc ccttaattac gctcatactt agatctccct
     8341 aatctcgtta agcaagcacc tgggaatcgg gagttcactc atctccagga gtcccggacc
     8401 agactcgatt acggcaggaa tcatgtttgt agcaagggcg atcgttccct ttccgcccgg
     8461 gatttcaggc tttattgaaa ggtgaatgtc tggttcgcca aatatgtcaa tatagtctcc
     8521 cgtgtcgatc ccttcctttt cgggaaggat ctgctgagga tgaatcagct ctatcattag
     8581 cttgtctccg cagtaacccc ggcctatatg cctgcatccg gccaccattc caggcaagac
     8641 atgcgcaacg ctcgtttttc tttcggtttt cgagatgatc ggctctcgac tctcctctat
     8701 tctgtcgata tcccatccca gagcgttacc gatcattgct atcgactgtt gaaagcctat
     8761 gtgccctaca atctctccag tttctattcc cttcttgaac tcctctggcg aggttccgac
     8821 tccttgggtt ttcatcactg tcctaccgaa gggcgagagg tcgtttatcc ttctcgcttc
     8881 gattctgtca accttcaagc atgcgcccgt catggctatt attagaaagt caagtacgaa
     8941 tccgggattg attcccgttc ccagaatcga tacaccgtaa cgccatgcaa tgttctcaag
     9001 cacctccgac tcttcgggat gcgagaaaaa cggaaaagcc atctcttctg ctatcgtaag
     9061 aatgtccaca tggtgcttgg ccacggtctc aatcttagtt agaacttctt cgacgaagga
     9121 atttgtagca ataaccacaa tgtccggtct cgtttcggat atagccttct ccacatctga
     9181 acagatctgc cgtccagtct tttctccacc aagatactct cccaggtctc gaccgatctt
     9241 ctgtggatcc ttttcaacgg caccaacaag cctgaggccc tccttctgag aaacatttct
     9301 tgccacgcca ctacccatgg cgcccaggcc ccataccaaa accctgtaag ccataaataa
     9361 acctccattc tgaactctag aatattcaat tcaatactat catcggaaaa aaggggaggc
     9421 tatttgagca aaatgcggct attcgtgaaa aaaaccgttt aaacacacct tgcgtgagta
     9481 tgtttgagta tgcgtaagta tggataagat aactagaggt gagaatgact cagtactcta
     9541 tggcgactct cttgatctct tcgagtgaag ttattccgtc tattaccttc tgaacgccgt
     9601 cattgtaaag cgttctcatg ccaccggata ttgcggcggc tgcgatctcc ctttcggaag
     9661 cgttttcgcc tataagctgt ctcagcttgc tgtcgactac aagtatctcg ctgatactgg
     9721 ttcttcccct gtatcccatt ccgcgacact cgggacagcc gttgccgggg atgtactcac
     9781 tgaaatcatc gcgattggga taaagcatag aagctatctt ccttacttcc tctctcacag
     9841 gaatcttaac cttgcagttg ttgcagagct tacggaccag tcgctggccc atgactccta
     9901 tcagggatgt gccaagaaga aatgggtcga cacccatatt gacaagacgg gccacggcac
     9961 ctgcggcgct gttcgtgtgg atcgttgaaa agactagatg gccggtaagt gaagcctcga
    10021 ttgccagttg ggccgtctcc ctgtcacgaa tctctccgac cattattatg tcggggtcct
    10081 gacggagaaa tgatcttaga tatctcgcaa aggtcaatcc tatgtctgtg tttacctgac
    10141 actgcgagat tccctcaatg gtatattcaa cggggtcttc ggctgttagt acgtttactt
    10201 tgtcggaagt aacctggttt agaacggcaa cgagagtggt cgattttccg ctacccgtgg
    10261 gaccggatac cagaatgatg ccatacggag aggtaatgat actttcaaaa cgctgtcggt
    10321 tgtaatccga aaggccaagg tcctcgagct gtttcttcgc attcgagaca cggagaattc
    10381 tcatgacgac cttctcaccg aaaatcgtcg gcatcgtcga taccctcatg tcgaactgct
    10441 cattgccgcg gcggatgtag aactttccat cctgcggcat tctcttttca gagatgtcga
    10501 ggttactcat aacctttatt ctagagacaa cagaattatg agcctttctg ggatagctca
    10561 gtatctttct cagaaccccg tcgaccctca tcctcgctac ggccatcttt tcataaggtt
    10621 cgagatggat gtcactggcg tcgcttctta taccgttatc gagaagtgag tttatgaatt
    10681 ttgcgaccgg tgtgtcatcg gtttcgtcta ccttttcctc gtcgagatcg acttctgagc
    10741 gctgatctat gctgaaggta ttgtcgatta tctcactcga agcagattct tcgtatgacg
    10801 acctgtagag agcgtcgaac atcgggggag gtacgagaag agtgctaacc ttgttcccgg
    10861 taaggaatga aagctctctg tcaatctggg gaaccttcag aacatcggtc gttacaactc
    10921 tcagaaggcc attttctgga agatactcta taggtatcac attcagcctg tcggcgacag
    10981 gcttgggaat catcctgaga acttctggag aaagcatgtt cggcaaatct tgcagtacct
    11041 tcaggccgta ctgcttcgat agggaatcgt agatgtcctc ccaggagatt actcccatcc
    11101 caacgaggat ttctccgatc ggcttattta cttttttctg tagagaaaca gcctgctgga
    11161 gagtgtcttc gcttatcaaa ccctggtcta ttaaaagctc tccgagtctc ttgtagactc
    11221 tcaacaatta ctcctcctcg ctaagatcca atccaaacct gtaaattgcc gggatatctg
    11281 aagcatcgcc catcagttca ccttcgtcga gttccagacc agccgcaatt aaagttatat
    11341 caagttcatc atcattcata tcggggtcga caataaggcc gagtttcaca tcggcatctt
    11401 cgctgcagtt ctgtctaacg atcgccgcgg cgatattcat ttctctaaga gttatattct
    11461 ttgcagagac gttcaggatt ataccggttg cgttatctat gggcttttcg agaagtctgc
    11521 tctcaagggc tcttcttgct gcttcttcgg cccgtctctc acccgatccg actccgatcc
    11581 ctagcatggc cgtgcctgca tttctcagaa ctgattctac atcggcgaag tcaaggttga
    11641 tgtagccgcg tttagttatt aactcagaga tacccttgat tccgtgatga agcgtctcgt
    11701 cggccttcgc aaatgcatcg acaatggaag tgtttggtgg tagttcctga agtagcttat
    11761 tattcgagat tcgaatcaaa gtatcgacgg agttcttcag ccttctgagg ccctcatgcg
    11821 cggtctttaa acgggtattc ccttcgaaga agaagggagt tgtgaccact gcaaccgtca
    11881 gaattcccat ttctcttgct atagaggcta ctatcggagc tgcacctgta ccggtgcctc
    11941 cgcccattcc tgcggtaata aataaaagat ctgtgtcttc gaggaacgta ccaatctcat
    12001 ctaccgattc ttctgcagcc ctctcgccaa cgttggggtt accgcctgct cccagacccc
    12061 ttgtcaactc cgtaccgagc tggatcttca ggtcggcttt attgctttca aggacctgga
    12121 tatcagtgtt ggcagcaata aatgtaactc cgtgaatgcc ttcggatatc atcctgttga
    12181 ctgcattacc gccggctccg cccacaccga ttacttttat ggaaggcaat ctaatttgtg
    12241 tgtttttttt gcctgtgtca atctcaaagc tcatcatcta cacctccaaa gaagagggat
    12301 ctaataaaag atgcaaaacc tttcttgggt ctctctacca cactttccac cgcttctccc
    12361 tgaatctctt ctggggagac cagattgccc aaacacgaac tgaatatggg atcattggca
    12421 acgtcgtggc tattctcaat tcgcctgtta aagctcgttt cgtacgtacc gattctcacg
    12481 ggcatcttga gagactcgac tcccacatcc gttaaccccc ggagcttaga tccaccgcct
    12541 gtgaagacca ctcctcccgg tattctctct tctgaatact cgggattgtt tatcacgaag
    12601 agctgaatct ctttgcgaat cttattcagg agttctttaa cccttgcata gatgactgtt
    12661 gagagtcttc tgacagagac attcttcctt gtcctctcgt ccagtccaaa atactctacc
    12721 acatcttcag catcgggcgg gaacatgctg cagtggccgt aattcacgag cagtctttcg
    12781 gcctcgtcga cagaagtacc caggactcta gcgatgtcga gaacgatatg tcgaattcca
    12841 agaggaattc tcgaaatgta tacgggaagg ttttctctgt agattaccat ttccgagaaa
    12901 ctatggccaa gaactacgct gaccacaccg tgctgcttct cggtatccgt gagtacggcc
    12961 tcggaggcgg agataagcgc aggagagatg aagaagtctc ctctgccaaa aagctcttcg
    13021 aagagcctcc ggaatatctc tactgatttg ccttcactgg aaacgaagac catctcgaca
    13081 ttgagtttct tagcgtacat ctcaaccggg ttgaaaacga ctttgtcatc gtcgagaatg
    13141 tacttgcgaa tgtaactcct gtgtatgttg gtgttcccgg tcttcatttt ctcggccttg
    13201 agtctgctca tcaaaccgac aatcgtctgt tcctttacca ttacctgttt atcttcagac
    13261 aggacttctt cgatattttc agagaaaacg gagtaatcac catccgtaaa acttatcttg
    13321 aagtccgctt caacgtcctt ctttcccatt tgacctgtaa gatcttcgat caacttctga
    13381 atagactgtt taagagcgac agcatccttc acttcgccct tgtcgagtcc aacagtctta
    13441 acgctcccat aggcttcaag agtcatctca cctgtctgtt cactacttac caccacacct
    13501 tttagggtgt tcgtgccaac atcgagagaa actgtataat ccttccccct ggccatcaat
    13561 gtcacctcgc tctcaacagt ttaccatcac tcaagaaaat atattcgctc ctgtcggagg
    13621 cgttttccaa ctgaagaagc agctccttgt ttgtatttaa actctcccaa tcaagtacct
    13681 taactctgat tcctctcctg agaagtaaca cgttattctt gaaatcaaag tacgatacca
    13741 gtgcaagaac tcttctgtca gcaagaatgc cctctaccgc tccggcaaaa atatctatgt
    13801 ctctttcgct ggctacataa actccatcgg ttagaatgaa ctgtattcca cccatgatcg
    13861 gatagcttag aatttcagaa agatgggcat agtctactat aacaaaatcc tccgaaaccc
    13921 agtagtattt gccctcgtaa ctgacgatgt acttaccagg tttcaaataa actctaccgc
    13981 ctgaaacatc aacacttccc ggtgggtaaa ttatatcagt tttccaggct cttctaaagg
    14041 ccagcggaaa ttcaatgaga atctcgtcct tatttacaat cctggttaac tgctctacgg
    14101 gttcagtgaa aaacgactcc agcacgaagt tcgctataca caatatccac atggcaaaga
    14161 gaatcaccat gcaaattccg gctctcagtg aaagactcat acgtcaagat tggaaatact
    14221 cattgcatgt cgttggataa acgaccttct ttcctccacc tcgctaccca ttagtattgt
    14281 gaatacccga tctgcttcct ccgcatcttc tatggttatc ttcacaagtt ttctgtcatt
    14341 tggattcatc gttgtttccc agagttgctc gggattcatc tcgccgagtc ccttgaatct
    14401 ggagtaattc agttttctgt ctgaatgctc cttaagaaaa gtcgtcaact cttcgtcgct
    14461 gtagaagtag tgcttttgcc tgttcatctc gatcttgtac aggggggctt gagcaacgta
    14521 gacctttccg tttgttatca gtggggtcat gtaacgatag aaaagagtca agagaagtgt
    14581 cgtgatatgt gcgccgtcta catcggcgtc agtcatgatg attatctttc cgtatctcag
    14641 attcttgatc tcgaaatcct cccccatccc ggttccaagg gcaacaatga tattgcttat
    14701 cgtctcgctt ttcaacatcc tgtccagacc ggctttttcg acgttcaata tctttccccg
    14761 gagaggaagt atggcttgcg tctccctcga acgtgccatc tttgccgaac caccggcaga
    14821 gtcaccttca actatgaaga tctctgtctc gtcgaggttc tcggagatac aatcggctag
    14881 tttgcctggc agggtcgtgt tttcaagcgc gttctttctg cggaccattt ctctagcctt
    14941 ttttgcagca agccttgccc tggaagcctc cagagcttta ctgaggatcg ctttgagatc
    15001 cttttgattg taatcgaagt actcttcaag cttttccttg actaccttca ttactgcttc
    15061 gaaggcctcg tcatttccga gctttgcctt tgtctgaccc tcaaactgag gttccttgac
    15121 aaagacgctt aggattgccg acagcccttc tcgaagatct tcgccctgaa ggttcggatc
    15181 cttgtctttg agcacgttgt gctttctgcc gagatcattc attgtcttcg ttagaaccgt
    15241 cttgaagcca gtcaagtggg ttccgccctc tatggtcttt atgttgttta cgaaagtaag
    15301 aatgtcttcg tcgtaggaag atgtgtactg cattgcaagc tgtatcttga catcgttgta
    15361 cgtgccttct agatagatcg gatccttatg gatggatttt ttgttcttcg ttagagcctt
    15421 cacgaactct acgattccgc cttcgaaatg atagctcctc ttttctccga ttcttctgtc
    15481 ttcgaaggtg atcttgattc caccgttgag gtaagcaagt tccttgaacc tcgcctcaag
    15541 aatatcgaag tcgaagtcgg taacggtaaa aacaagtgga tcgggcttga aacttgttac
    15601 cgttcccgtc tcgttggtct ccccgacgac ttccactgga gcaagggcct tgcctcgttc
    15661 atatttctgc ctgtatatct tcccatctac ctttacctca acgatcatcc actcggacag
    15721 ggcattgaca accgaagcgc ccacaccgtg aagacctccg ctgatcttgt aagagtcctt
    15781 tgagaacttg cctcccgcgt gaagcgttgt cataacaact tcgagcgcac tcgtcccggt
    15841 atcgggatgg atgtctatcg gaattccacg cccattgtca ctaacaatta cagagccgtc
    15901 ctccgtaatc actacgttaa tggtgtcgca aaatcccccc atcgcttcgt cgatactgtt
    15961 gtcgacgatc tcgtagacaa gatggtgaag ccccgacttt cctgtcgacc caatgtacat
    16021 tccgggtcgt tttctgacgg gatccagtcc cttcagaatc tttatgtttt gtgcgttgta
    16081 taaatcagac atttaacacc tccaggtcta ccgtccaatt ctaaatctga ttctctttat
    16141 tgcttctact cccagtgaag agttcatcct ttcaagcagg attttctctc gcagtttcat
    16201 ctcattaagc cacataccgt cgtcgactcg aattatcagg gttccttccg agaaatcgac
    16261 gatcgacgag tgatttgcga taggctctcc aacgatctct ccccaatctt tgtttagctc
    16321 aaaaacccgt atctttctga acaggcttga gctcttcgcg agatcgttaa atatctgccc
    16381 tatagttcga tggttccact tcatccaatc cgctccagtt actatcctcg gtcattatat
    16441 atccttcgaa attatctagc agcagtctag tcataccatc ggtgaagtct tcaataagca
    16501 ctttatcttc aagtcttcgc accgggacga tcccgacgct cgtgtgagtt atgaagatct
    16561 cgtcggcaca gaaaagctcc catgcgggaa ccggtctctt ctccaccgaa atctccagcg
    16621 attcgcacag cccgattacg ttgtctctgg taattcccgg aagtaccccg gatcttatgt
    16681 cgggcgtaac aactgaaccg tccatcacga ggaagacgtt cgaaaaggag ccttcacaga
    16741 cgttaccctt ttcattcaaa atgagcatgt cgtagttgtc cccgagcgcc tttctcgtcc
    16801 ttatgatcca gcttgaaccg ggcattttca gttgaggatt gactatgtcg gcggagggct
    16861 tcaaaaaagg tgagattgag atctttacgc ccagttcgta gatgtcttta gatggtgaag
    16921 gaaggtcact gaaaataatc gctggtctac ttcgtgctcc atcactgata agggagattc
    16981 gaattctgaa atcctgggtc tgaaatccct tgtttttttc cagcccctct atgattattg
    17041 agttgagttc cttgcagcta aatggaactt ccagccccat cagagcagcc gactgcttaa
    17101 gtctctcaaa atgctttctg aatgcaaaag gcaatccgtt gtacgttcga actacttcat
    17161 agaccgactc gctgttcatt acgcccggat agagaacact tacgagcgaa gactgttcat
    17221 cgcaccactg atcggcaata tagttcagca tcggaaccct ccctttgact ctcaatagaa
    17281 attatcgttg tattcacaaa gccttcgtgt gccccttcaa ttgcatgagg cgcatagaac
    17341 tcatcgaatc cgctgctttc tgtatggcct gtcttttgga tcaaaacact tgcctttctc
    17401 ccgatttgtc tctcgagata ctttttcctt gatctctttc cagcctcgat tagctcgtgc
    17461 gatctagctt tcttcgtgcc tgaatctatc tgatccttca tctccgctgc ttccgttccc
    17521 ggtctcctcg aaaaacggaa gacgtgcacc ttcaagaatt caacctgttc aacgactttt
    17581 agactctctt tatggtctga gtcattctca cctgggaatc cagctataag atctgtggtg
    17641 aatgcgaatc ttgtatcgac cgatctggcc ctatctactg ctcttaggta ctcatctaca
    17701 gtgtacttcc gtctcatcgc tcttagtatt ctatctgaac cgctttgaag agagagatgc
    17761 aggtgtctcg ccatcctccg agaagtagtg attaaatgaa ggagctcacc gtccactcta
    17821 tccgggtcca gggaacctag ccggattctc cattctcctt cgagcttatc gagctcgatg
    17881 agcagtttca ccagcgagga atcgagatcg aagccatagt acccaatatt gattccggtc
    17941 aaaacgatct ctctgtaacc tctggaaacc aatccttcaa actccctctt caccagatct
    18001 attggtttac taataatctt gcttcccctt gcgagcctca ctctacaata agagcagcaa
    18061 ttcaggcagc cgtcctcaat ccctaggaag gccctcgttc ttccttcaat gcttgaagtg
    18121 acggttagaa attcacttgg tgcaactccg tcggggcgat taagctgacg agatacatac
    18181 ttgtgtattg cctttttttc tctgactccg attacttcat cagctccaag ttctcttaac
    18241 tcattaggag aaagctgtga gtagcaaccg gttattatga tttttgagtt gggattaagt
    18301 cccttcaacc tcctgaaaag ctgcctgagt tttcgttctg cttcggaggt tactgtacac
    18361 gaattcacaa tgaagagatc tgccttgtcc tgtgaaaagc ccacttggta atcgtgcagc
    18421 atctcggcca ttgcctggga ttcgtattgg ttcattttgc agccaaacgt atagattgaa
    18481 accttttgct tcatctattg atcttgcctt gagtcgctac gtgtataaga taaatcctgt
    18541 ttactattcc cactagcttt ccttcatcgt ctacgacggg catgatttta agcccgttct
    18601 tgatcagaag gtcggctgcg tatataaccg tatcgtcggg ctttataaga acaggaggtt
    18661 tgaccatgtg ttgagaaacg tgatcatcct ttatcttgcc gagccggata acgaactggt
    18721 gagtatcggg aatgaacgat gccgattgaa gaaggctgaa gtaacttggg agagcgcttt
    18781 tgataacgtc gctttcgctc aagaatccca caaccttgaa ctcctcatct accaccggta
    18841 atcctgattt attctgtctc aagcagaata caatgaaatc ctcgatcgtc tcatcttcga
    18901 agattgccga tacgtctctt atcatagctt cttttacttt catagagatc actccagctt
    18961 tcggatatcg aaggaatcaa gatgtcgctc gatatcctca agtgagataa gttccttacc
    19021 tatatattcg gcttccgcaa cggcggcagc cattcccctc ttcgccgcca gaaagcaatc
    19081 gttctcgttt tctatcatgg aatgtactac tcctgccatg aaggcatctc cggttcccaa
    19141 aaggtgagac ctgtctatgt ggcccttggt tttgaacaac catattccgt ctctcgtggc
    19201 tatgacgtcg ccttcaatag cataagaaag tatcgccaat cgggaaccgc tttcaataat
    19261 cttcttcgcg gtcctgacgt aatcttcgag ttctaccatg gtctcgccga ataacagttt
    19321 cctgcttctc aaatcaggtc tggcaacggt gataactcca gcctccacag ctatttcgaa
    19381 tgccggcccg attccttctg caaaggaaat cttgcccgag tcagcgactt tcttgcacag
    19441 agtcttacag atgtcgttgt ctacgcctct cggaatgctg cccgagacga ctacatgctt
    19501 cgcccttgaa agagcgacct cgaatcttcg aatgaacatc ctcaaatcat cagacttgat
    19561 cagaggtccg gatgagttaa tctctgttat agtctcgcca agcggatcga cgattgctat
    19621 gttttccctg gtctcttctt ctacatggac aaaggcggtt gtgatcagat cactgatcaa
    19681 acgcagtctc tcttcaacga cttttcctat gtatccaccc agaaatccca tggcaacgtt
    19741 ccggactccg agaccggaga gaataaccga tacgtttatg cccttaccgc ctgggtccat
    19801 taccgagtag ctgggattct tcaccctgtg gaactcgtta atcttgaaat tttcaacaat
    19861 gatctccctg tccagtgcag gattaagcgt aacggtaaga acgtccacgg ttcacctccg
    19921 atggggagaa attatcggtt gttcaaaacg aaaacttcgg gacttccgac atatgaaatt
    19981 ttaccatttt taagaacaat caacaaatcg gaaatgtcta gaagcctcaa aacaaacctt
    20041 gaagagatta tcattgtcac accgttcctt gtatccaaaa gaacgttttt cacttcagta
    20101 caggcgtcgt catctaggtg gtctaaaatg ttgtcgagaa tgattatctc cgggtgtcgt
    20161 atgagggtgg agaatacgag tagcgagagt ctcttgaaaa gaggtattgt ctttagagag
    20221 gtttccgcct tcagtgaaag caagtccagg agatcgagtt catgaaagag gtccaattcg
    20281 ctttccgcga agtcttcgaa ctcaaatcgc ttgcctttca aaaacctgaa gaattctatt
    20341 agagtaaaat tagacattgc ctcgagaaag gtcgtgtcga cgtaagcaac tctcttcctc
    20401 acgctctttc tttcgaggga gtctatatcc tcgccgaaca gcttgatcga tcctgtatag
    20461 ctaactctgt caaacagttc ttcgttcaag tgaatgaaac ttctgagaag cgccgattta
    20521 ccagcccctc ttgctccttg caagagtgcg atgctcccct ttggcacatc aaaggtgatg
    20581 ccgtcgagca tcttccttcc ctcgacgagt agagtaaaac ctttcaatga aagagcagtc
    20641 tcaatcacga gactgctcct ctttgccgtt cttatcgttc aagcgactaa acatttccgt
    20701 gctggcgagt aaatcgccat tcttagcttt tttcaataat tcaatttggg aaagaatcct
    20761 gggacttgtt tcaaccggtc ttgctttctt tttctcaacc aagttttcac gtcccttcgc
    20821 tggtgtactt tattataaac atagactctt tgctctgaaa tgatgcaata attaacagcc
    20881 tgtaattaaa tggctaaatc agtcgcgctt ggacgtgttc agcataccca ggagagcctt
    20941 gagctgcttg cttctggaag gatgtctgag ctttcttagt gcctttactt cgatctgacg
    21001 gattctctct ctagttacgt taaaaaattg accgacttct tctagagtct ttgtctttcc
    21061 gtctagaagt ccatacctca tcttgagcac catcgcctct ctcggcgaaa gagtatcgag
    21121 aatctgatcg atctgctctc taagaagcat cttcatcgcg gcttcctctg gctgttccat
    21181 ggagtcatcg tggataaagt cgcccatagt cgattcttcg tcgccactta tcggcgattc
    21241 gagagagacg gtttctttcg aggcggcgag gatctcctct atcttttccg gtgtcttgcc
    21301 ggttaggttt ccaagttcct ctatcgttgg atactcacca tggtcctgca agtgttcacg
    21361 tattactttg tttagcttgt ttatcgtttc aaccatatgg acaggaactc ttatagttct
    21421 tgcctggtct gcaatagctc tggttatagc ctgtcttatc caccatgtgg cgtaagtgct
    21481 gaacttgtat ccctttttcc agtcgaattt ctctacagcc ttaataagcc cgatgtttcc
    21541 ctcttgaatg agatcaagga aggtcaaacc gcgtccgata tacctctttg cgatggaaac
    21601 taccagtctc aagtttgcag tgatcagctc ttccttagcc ttcggctctc ccttttgtgc
    21661 ccgacgggcg agctcccttt ccctgcttgg cgtaagaaga gagatcttgc caatttcctt
    21721 cagatacatc tttattgggt cctttaatga tatgttgtca tacatctcag gcgtagtttc
    21781 aagtatcgaa acaagctcct cgctgctttc gctttctatg tcatcgtcat cttcaactgg
    21841 ctccttttcg acgatattga tcttgttctt ttcaaattcc tcgtatatcg attcaacaag
    21901 actagagtca aagccttcgt aatccggcgg aaaagcgctg tctatatctt cgtatgtaac
    21961 aaatcccttc ttctttccct gtctaagcag tttcttgatt cgtttgtcta tctcttcctt
    22021 agtcattaga gcactgttat cgcctgtttc atctgaatcg gcttcgtcat ccgctatttt
    22081 ctcggcaaga gatttgatgt tatccgcaat aaccttggcc cctgactttg ttttcttctg
    22141 tttttccaaa ctaatcgcca cccttcatct ttctctcgag cattcttctt agctcgatcc
    22201 tcctgatcag caaagccttt cttgccccct catccttgac ctttggaagt cttcggtcaa
    22261 tctctgccat ctcctcttcc acaagtctct tgttggcata caatccggtt gactggagaa
    22321 cacgttgtgc ggtatcaaaa tcaatgcaag tagatgccag ttcgatcagt tctctgccta
    22381 tactttcttc caccagatcg agtaactcct caagttccag accctttttc attcctttaa
    22441 ggagttcctt caggtctctg ctttcattca tcaactctag ctgtttgaca accgttgccc
    22501 gcagctttgg atgctgcaaa tatatcctta tgtgatcctt gagtttcaga gctaaaccgc
    22561 ttttcatttc cccgccggag ctcttcgaca ttccaccgcg aagtgcggat tcaagctctc
    22621 gctccgaata acctgtcttc tgtgcaagcg ctgcaatcgt cgactggacg gcagcaaggt
    22681 ttccagtgct tctgaatgag atcacatatg gtttgagatg ctctatcagg tgctttcggc
    22741 ccaaaggaat cgacagatcg agttttctcg aaaagaactc gactctgaag gcagggcccg
    22801 gaatcgcaag cgcgagggtc tcctttatcg cctttggacc ttctgcaaca aacaaatcgg
    22861 ccggatcttt ctgatctctc gaaaaaacaa cggccgttcc aaagtccagc ttctctgcaa
    22921 tgtctatgga cctaagtgat gctgtctgac cagcctcgtc ggaatctaga aagaacaaga
    22981 ggttacgcgt ataatttccg agaattctaa gatgcctttc cgtcagcgca gtaccgagta
    23041 ggcccaccga gttcgttatt cctgcctcga acagggaaat gacatcaaag tacccttctg
    23101 cgatcaccgc gtagttcaac tgcttaatgg ccgccctggc acgtgaaaga ttgaataaga
    23161 gtctattttt ctggaagtat ttcgactcgg atgagttgat gtatttcggt cccttttcgg
    23221 catccatgat tctaccgccg aatccaacaa ctttacccga ttcattgtca atcggaatca
    23281 taagcctgcc agcaaatcta tcgctatagg tcgcccctct cctcaccaga atccccagtt
    23341 gaagaagagt cttttcatcg gttctaagtt tcgacggcag tgactgcgga aaagttcttt
    23401 catccggtga ataaccgagc tgaaattcgt ctatcgtgtt ctgggagatc cttcgctttt
    23461 ccagaagata ggccagtgtc tcgggcctct tctcttttag ttctctattg taaatacccg
    23521 caagccgaga catgatcgtt gtgaatttat cgtactcgga ctcggccgta tcgacctgaa
    23581 tgggaatgcc tgcataatcg gcaagtctct taactgcctc tgcgaacgac agattctcga
    23641 tcttctgata gaaagaaatc acatcacctt tttcaccgca gccaaagcaa tgaaagaagc
    23701 ccctttgagg gtggacataa aatgaagggt cgttgtcgtt gtgaaacggg catagtcccc
    23761 tgtaagagct tccggccttc tgcaggttca catagcgccc tatgaaatcg acgatgttta
    23821 tggacttttt tatctcgtcg agttcctctt tggtgaacac tgaatcacca ccggcaaaaa
    23881 aaggcccata cgctctatgt acgggccgta tttttcgaaa cgtgttaacg cttggagaat
    23941 tgaggtgctc ttcttgcttt cttgaggccg tatttctttc tctcgacttc tcttgggtct
    24001 ctcgtcaaga gactgttctg tcgtagaagc ttcttgagat caggattgaa cttaacaagc
    24061 gctctggcga ttcctagact tatcgcacca gcctggccgc caagtccacc gccgtttact
    24121 cttatgatca tgtcgaatct acctatggtg tttgtgacaa cggcgggttt cagtgcactc
    24181 ctgacccatg cgtcatttga aaggtattcc ttaagatcct tgtattcttt tccatttatt
    24241 aagagcttac cggttccagg tctcaggtga actctcgcaa cagaggtctt ccttctgcct
    24301 gttccgtaat aatcaacaaa gtcagccatg cctcaacctc ctcatttaac cagttcgata
    24361 agttcgggct tctgagcggc gtgttcatgc tcgggacctg catatacctt gagtttctta
    24421 agcatctttt caccaagagc cttcttgggg agcattctct tcactgcaag tcttacaacc
    24481 ctatccggga acttgtccat catctgtctt gccgttcttt cctttatgcc tcccggataa
    24541 cccgagtatc tgtagtactt cttttggtcg agtttcttgc cggtaagctt aaccttgtcg
    24601 gcattaacta ctatgacgaa gtcaccggta tcaagatggg gagtataata tggcttgttc
    24661 tttcccatca gaaccattgc gatctgtcca gcaagtcttc ccagcgggac atcagtcgca
    24721 tcgacgacgt accatttctt ctctatcttg ttgccattat gatcaaccag ataaccgagt
    24781 gtcttctcga tttctccctt tcttttcgtc tgcaccttgg agtgggttac tgcccatctg
    24841 cacaaaggtg tcttctgaat cttcatagat gcaatcctcc ttaaccgaac agcctactcg
    24901 ctgtagacac taacgtactt gcggttgttc ttctcttcaa agtgaacctt gccatcaata
    24961 agagcaaaga gggtatggtc tctgccgagt cctacgttct taccggcgtg aatctttgtt
    25021 cctctctgtc taacaataat ggaacccgcc ttcacggctg aagattcgcc tcttttaatc
    25081 ccaagatatt tgggattact ctctctcccg tttgaccttc ccgcgctttt tcgggccatc
    25141 tcatttcacc cccgtctcga tcttctcaat agagacttcc gtgaaccact gtctgtgacc
    25201 gtttactctg tcgaagttct ttcgggggcc aaacttgacc acaagcactt ttttgtctct
    25261 accgtgagaa acaaccttgc ctacaacttt tgcgccgttc acataaggtt tgcctaccgt
    25321 tgcttcttct ccgttgtgga cgagaatcac tctgtcgaag actatctcgt ctccgctttc
    25381 ctttccattc tgcctttcgg tgaagagagt catgccttcc tcgactctat actgtcttcc
    25441 cgaggcctca atgatggcat acactaatag ccacctcctc atgcaataga aatgtgctga
    25501 aaccaggtac ctttcggatt ttagacctgc ttcatgcacg aaaaaccact cgcgatcaaa
    25561 attctatcat aattggcagt gacagaacag tgtaagagta acgtttatat tacgaacgag
    25621 aaagagtttt gaatagatga gatctctctg actcccaagt taccatattg acagccctat
    25681 tgcccgtgat ataatcagtt acgaaaacgg aggcgtatcc taattggcta aggagccggt
    25741 ctcgaaaatc ggtggggttg acgccccttg tgggttcgag tcccaccgcc tccgccagcg
    25801 ggccgttgcg cccttatttt ttggaggtgg tatcgtgatc ctcaaggaga tcctagagct
    25861 tctgaagagt aagaagggta agatggatta ctccattcca aaggagtggt tacctgacgg
    25921 gtactcaggt accttgaagc ttagagatcg atacatattt gtggacccat acgaattcgg
    25981 ctcaagcata attgaaagca tagccgctaa agccgaaccg ggagtcgatt attcgaagtc
    26041 gctgggcagg attagaaacg agagcgacaa tctttggatc aaatccgcaa tcgtctacgg
    26101 atcattcgtc aggtcgaccg cagcctactc gcatcaagac ccggaattct tctcagatct
    26161 ccattcgcaa gaatactcgg aatcagggac cttccttaag atgatctata tgcttccata
    26221 tctatcgaag atgggcttcg acactcttta cttcctccct gttaccagtt acagcgacaa
    26281 gttcaagaag ggagaactag gctcacctta ctccgtgaag gatttcttct caatcgatga
    26341 acgctaccac gatgtcttgc ttgaggatat ggacgtcgaa cgcgaattca aggcatttgt
    26401 cgaagcggcc catattatgg gaatgaggat cgttctagac ttcatcccga gaacttccgc
    26461 tagagactct gccttaatac tcaaacatcc cgagtggttc tactggatag acgcatctca
    26521 tcttaaggac tacaggccgc ccaagattga tggtctcggc ttcgaccagg ccagggttga
    26581 gacgttgcct gtcatctact cgaatgagaa cgtgaagaag catctggcga tgttcaggta
    26641 ctcgccggag aaactcaatc cggaaaagtg gagaaacttc gtcgcccacc acgaaggcag
    26701 cgacaacttc cttgatgagc tcgtttcgga gttcggcgta ataacacctc cgggtttttc
    26761 cgactggata aacgacaacc agcccacctg ggacgatgtt accttcctga ggctgtacaa
    26821 agatcatccc gaggaatcac ggaaatttct ttcagaggac cagcctccct acgttctctt
    26881 tgatgtagta aaatcatcct tctttcctgg gaaagagccc atgacggatc tctgggaaac
    26941 cattgagaat attatgccct tttacaacag taaatttggc gtcgatggcg caaggctcga
    27001 catgggtcac gccctcccca gagagttaga gcttagaata attcgtaagg ctaaggagaa
    27061 cgatgcttcg tttgcaatta ttgccgaaga acttgtgatg gggaatgacg agaaggcgag
    27121 acagtctggc tacgactgta ttttggggaa tacatggtgg atggaaccga gaataactga
    27181 gggaaaattc aaagaacttg tatacgagat tcttcccaga ctacgcgtgc cgacgctggc
    27241 ctgtgttgaa actccagaca ctcccagggc agtcgcgaga ccgaacggga agaagttttc
    27301 gcgctttgcg accgttctga actacttctt gccaaacgga ataacttacg tcaattccgg
    27361 ccaggagata ttcgagatac aaccgatgaa tctaggcctg gataacgacg aggacggcag
    27421 atatgtcctt ccgccaaacg atccattcta cggcaagctg gctttcttcg atccgtatgt
    27481 tctccactgg aacgcggatg acgacatggt cgatctgatt tcccttctat cttcactgag
    27541 aagagataat cttgagctca tcaaccccca tgatttgaaa cttctctggg aggaagacat
    27601 cgaatctgca ggactcttct actggaatgg gtcgaaggga ttgctggttg ttgctaatgg
    27661 tgacttccaa agcatgaagg attttcacat aaacctgggt tattttacct ggaagagcga
    27721 acacaaagtt gcgtggaagc tcaagggttt cgatggtcac tcggaatgga aggtaggagg
    27781 cgactttcat ctctcgttag gtcccggaga ggtggcagtg gcagaaatca aataaaaaaa
    27841 cgggccccga tggggccctt cttatatcta ggaattactt ctttggctgt acatagaacc
    27901 taactgccgt tcttacttct ccgtcgatgt caacatccga aaaggttgga acacatacga
    27961 gatccatacc gcttggagca acgtaacctc tggcaattgc tatggccttt actgcctgat
    28021 tgaccgctcc ggctccaata gcctgaatct ctgctacgcc cttctctctg agtactccgg
    28081 caagtgcacc agcaactgcg tttggttttg agtgggttgc tacctttagt acttccatga
    28141 ctgctacctc ctcttgagat ctgtcatgct actgggagag agatttagga tggtgcgccc
    28201 ggcaggattt gaacccgcaa ccatcggagc cgaaatccga tgctctatcc gttgagccac
    28261 gggcgcatgg aaaaagagaa aaatggggtg gctggcgggg ttcgaacccg cggccgcctg
    28321 atccacagtc aggcgctctt ccaactgagc tacaaccacc atagaaattg cgtggcgcgc
    28381 ccggcaggac tcgaacctgc aaccaccgga ttagaagtcc agtgctctat ccattgagcc
    28441 acgggcgctg gagcggccga cgggagtcga acccgcaaca ttcgccttgg aagggcgata
    28501 ctctaccaat tgagctacag ccgcactaat gacccttggt cgggaagaca ggatttgaac
    28561 ctgcgacctc ctgtgcccaa gacaggcgcg cttccaactg cgctactccc cgctcacgaa
    28621 tctaaatata tcagatttta aggatgtcgt caatagtaca gaatccaagt tataggcgaa
    28681 cggggattag cacctttata attctctccc agtaacttgt caaagatcta ggctaaaaat
    28741 tagactgtgt cgtgatcgtg aggttcaaca tatcacaaca tcgtgagcca tatacaggaa
    28801 atgtagtaga atattcttga ggtggttcta attgaatgga atcgttacat cgaatctcgt
    28861 gaaaagatac ggttcgtttg aggctttgaa gggcgtttca ctttcgatag aagacggcga
    28921 tctgtactgc cttctggggc cgaacggtgc aggcaagtca acccttataa gaatccttac
    28981 tggattgatg aaaccgacgt caggtgaagt gaatattgcc ggacttgata tgaaacgaga
    29041 tctgaagaag attagagaga aagtcggtct tgtgtcggag cgagtaattc tctatgatcg
    29101 tcttactccg gtcgagaatc ttctattctt ctcttcaatg ttgaggattg agaaaaggac
    29161 tgcgctgaaa aggataaatt ctcttttaga aaaggtagac atgatcgagt ggaaagacaa
    29221 acctgtaagg gttttttcga caggaatgaa gcaaagagtc aactttgtaa gggccttgtt
    29281 gcacgagcca tcgattctct tcatggatga accgacactc ggactggatc ctcattcgac
    29341 caggactata agaaatatgg tcagagaact caatgaatcg ggaagaacga tcgtgctgac
    29401 cacccatatc atgagtgaag ctgaaaatat cgcgaaatca gtaggaataa tgaataaagg
    29461 caaactcata acgaccggaa aactggaggc catccgcgct tctttgaata gagaccgcct
    29521 tactataact gtgaaaaaca gaccagaggt tccatttgaa tctatagatg gctacatgag
    29581 cgatagccgg ctaaacggag ttgtgaggat agatttcagg ccagggacaa ctatggaaag
    29641 cgttcttccc aagatactcc agatgaagct cgaagtgata gatgttgatc acataaagcc
    29701 ttctcttgaa gacgtattcg tcgagcttac cggagagaaa tcatagagct agaagactcc
    29761 acggatcgat cttcaaacca ccttcaagtc ttgacttttc tgcagcccag cacatgaggt
    29821 gactctgaat cgactctttg aacgaagttg acagcccagc cgtttcgcct ttaaggtatc
    29881 ttacgaaatc tctagtaatc tccatgtctc cgccgccgtg ccttgattcg tcatgtgata
    29941 tcttataaat cttcgtctct cctgagaaca atctcagctc gactctgtcg ctttcgaagt
    30001 ctccgtagat ctctccgtcg cttccgctaa tgaaaatctt tcttgtcttt gaacttgtga
    30061 aggcattcat ggagaaagat gcttccacac cattttcgaa cgagaccaaa acgttctgat
    30121 agtcgacaac gttgttatcg cttctgaaaa cgcatttgcc gtaattaccc gtacggagaa
    30181 catcccttct cttctcgagt gacatatcat ttccaatcgt attcactggc cagtcggtat
    30241 tatctgaaag atactgggtg acggccgaat acgggcaggt tccttttaga ggacagtcca
    30301 ggcaccttcc agtagaactc tcgggtgcgt tctctctacg gaagtatttg aggccaccgt
    30361 gggaaacaac tgaaagtgct cttgacgaaa gcaaccaggt gaggatgtcc atatcgtgac
    30421 agctctttga aaggatagaa ggacctgcaa gctccgatct tctccagttt cccctcacgt
    30481 aagaatgggc gaagtgatag tatccgacct gttcggtgta ctcaacagca attaactttc
    30541 cgatagcttc agattcaaga agcgacttga tcgttttgaa gaagccggag tatctcaaaa
    30601 cgtgggcaag aaaaagcctc gacgtatctg caccggagct tatcaaatcc cttatgccgc
    30661 tcaaatccgc agagatcggt ttctcgagta gaacagcgta accacttctg agagcttcga
    30721 ttgccggctc gacatgcacc gcctcaggtg aggcgacgat aacaccatct gcaaacctgg
    30781 gtcgcgaaag gagcgacttc cagtcagcca cagcattttg aggaattata gaatgatcct
    30841 ttgcaaatct ctctcttcta aactcgtcgg gctcagccac agcaacgacg cgcgcatcgc
    30901 ccattgaaag gacagcctca ccatatgcga aataccctct atttccagcg cctatcataa
    30961 ttagatctat caagagaatc gaccaccttc atcttcactg aatcgattct atcactcaat
    31021 tcttaaagag aaagataggc aaatttccct tgatgtgtta taaagggaaa gatcaaaaac
    31081 gaaaggacgt gacgagtttg acagacataa aaagaaacga cacagagatc gtaagagtct
    31141 ccaagagaga atacaaaggc catgaattca ttgatttgag gatctactat caggacgatg
    31201 aaggagacta caaacctact aagaaaggaa tcacaatcaa cccaaaactc gtcgatgaac
    31261 ttatagacgc actcaaaaaa gaaaaagact ctgccccaga aaaagaataa ctctcatgcc
    31321 gtcgccggtc acgtaagccg tgatcggctt tcatgaaaga gctttaatcc ttcataagaa
    31381 ctggaagtcg tttataagag cttgatttat agtgcttctt aatccatgct gttggctctt
    31441 agaaagaaac aaaaaggaga atcatagacc aaacggaatc tcacatgtaa gcaacgctgt
    31501 gcgctggaaa gagccgtcct tcggtgctcg tccaagacca tggacccgtc cttcggaaag
    31561 actggttctt cgttccgacg ctgcgcgtcc aggttgttcg ttcttgaaca gatcccgtat
    31621 caagtccggg atgacagtgt taggcgattg cgataacgtc acattccgcc atcctcaaga
    31681 aactgtcctt cgtcaaggat catgaacctg tccctcgaaa gagccgctat ccatttagga
    31741 acaagaaccc gctgtatgcc gatgaaaacc gcgttgtccg tcaacggtcc acggtcctcc
    31801 gagaggatca aaacctagat cccgaaacaa gttcgggatg acacagtgag aaacttccca
    31861 aagccgatgt cgggataact tcctcagttg cattgaaacc ctgaattgat tgccggttct
    31921 cttcacgaga ccccataccc tgtaatcgat accctgccct tcgcgtcatc tgaaattgtt
    31981 tcagcataat ggttgtggtt cttgaacatg gcgaagaacg aagaacagat cctcgctctg
    32041 gaagaacggg ttctccttgg ggaacggaga accgttcaac ggcaaaccgg tttcacttgg
    32101 ctttttccaa cgccctacgt cctaccgcaa accacgctat atcccttcga aaagcaagat
    32161 cccgaaacaa gttcgggatg acagcatgcg tgacttctga aaaatcatgg cggtttgaaa
    32221 gcgtttgtta attccgggaa catctcaatt cgtcatgaac ttgtttcagc atccagctcc
    32281 tatttattct tcggagaacg gagaaccttt caatggcgaa ccagttcctt tgctctttcc
    32341 taccacgtac ccgctacccc caacaccggt ctaacaactc acaacttggg actgatcctt
    32401 gctctttcca gcctcttact actgaccact aaccaaggtt tctggaaagc ccagatcctg
    32461 aacaggagca tttcaggatg acgtgaaggg ctgtcacaca gtaagccttt gcgatgttat
    32521 cacgtttgcg tgcccctaaa tctactcact ttgtcatccc gcag
//
LOCUS       DAOQYI010000019        32178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1628_length_32178_cov_79.315350, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000019 DAOQYI010000000
VERSION     DAOQYI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_32178_cov_79.315350"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1155
                     /gene="nagA"
                     /locus_tag="PKL93_06580"
     CDS             49..1155
                     /gene="nagA"
                     /locus_tag="PKL93_06580"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNQ70959.1"
                     /translation="MELERILVVDPIDGEYTADVRISGSKIESIKKTGGDFRSIAMPG
                     FVDTHSHGALGINCMTMNHEGLEKWEEFAQSHGVTYLLPTTVSASEEGMKRVADLVSK
                     YISDRSRTVVHGVHFEGPYINPKKKGAQNPEAIRQPTLSEINSVLSDVVMIITMAPEV
                     GGFLEVLPEIIKREIAVSIGHTDATYNQIKAAYKLGVRRMTHFPNGMNTLHHREIGCV
                     GAGFLLPLNLEMIVDGVHTTPEFVEMVYKIRGSEAIILVTDSLDATGLDNGIYDLGGL
                     RVEVKDNVATLEDGTLAGSTLTMDKAIRNFKRWTGCNLQELAKVSSYNALKNLGISNR
                     GRFKEGLIADIVLLSKELKVEETILAGKSVFISK"
     gene            1596..2261
                     /locus_tag="PKL93_06585"
     CDS             1596..2261
                     /locus_tag="PKL93_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNQ70960.1"
                     /translation="MELHKSTIAYSLKRELDYLLSLPLQYDEEDTEWPLILFLHGAGE
                     RGDNLELLKKHGIPRVVSEMSDFPFITVSPQCPENDWWLNRLQDLRFLLESIIKQYRV
                     DHSAIYLTGLSMGGFGTWHMAVEYPDLFSAIAPICGGGLGILGFPERVIDIKDLPVWA
                     FHGEKDNIVSVEESRILVKTLKEAGGNAMLKIYPDAGHDSWTETYSDPELYEWFMSKR
                     KLG"
     gene            complement(2253..2600)
                     /locus_tag="PKL93_06590"
     CDS             complement(2253..2600)
                     /locus_tag="PKL93_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70961.1"
                     /translation="MGFAVRRSRRPKIGLIRNNFIRASVLLGIVTPVSIYGITRFFGA
                     RERLEGFGWLLIMLIAVWILVAVIWVLSLRVYFEARKQIVRSRRGFRTGNLKPSRMIL
                     NRSQVSSIRTKST"
     gene            3391..3528
                     /locus_tag="PKL93_06595"
     CDS             3391..3528
                     /locus_tag="PKL93_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70962.1"
                     /translation="MSNSTVPDYGIQLLYEENETVGLFFSETSSERLLEIGPLALLPG
                     F"
     gene            complement(3701..4186)
                     /locus_tag="PKL93_06600"
     CDS             complement(3701..4186)
                     /locus_tag="PKL93_06600"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNQ70963.1"
                     /translation="MAEISLGKNVPDFSLLDQNGNEVSLSHFSGKKIVLYFYPKDNTS
                     GCTLEAEGFRDLASEFAGLNTVIIGVSKDSLKSHQSFSLKLDLNFSILSDEDGRIHEM
                     FDVIKPKKMYGREYMGTERSTFVINENRILAKEYRNVKAKGHAEEVLEFIRNLERSRQ
                     Q"
     gene            4610..5953
                     /locus_tag="PKL93_06605"
     CDS             4610..5953
                     /locus_tag="PKL93_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNQ70964.1"
                     /translation="MEFLAAQLPEKIGLLESNGDYKGAIKEIDNELESYLPSIMTRRL
                     LWEKERILRLKDNYPYTNSQAFEILSNSIDNFTREEFEGLCEAKQLDSMTLDGEARFE
                     RRFDKNLLFVMPEYGSRLKQKDNERDEIRVLLHREIDRLISTREPSKFKVNARSSINV
                     QSSEENVFRCWLPVSRNGEQVSSTKILNTSHSYFLSPADYSQRTVYMESDAKKDTVFS
                     VEFEYEISEISTSLDPSRCTHPDEKRFESYLIERAPHIVFSPFMRSLADEISGKETNP
                     YYIAKSFYDWICEHVKYTFMSEYALYPNLSEFAATNLRGDCGVKALLFITLCRIKGIP
                     ARWQSGWFASPKGASPHDWALFWLEPFGWVPADCSFGGSRKDIPAYREFYFGNLDAYR
                     MISNSEFMAPLLPAKRFFRSDPYDNQVGEIETEKRALRSYERECSIEILSFERLT"
     gene            6207..6839
                     /locus_tag="PKL93_06610"
     CDS             6207..6839
                     /locus_tag="PKL93_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70965.1"
                     /translation="MKKKFVLLSLLVIFAVSAFAHVPAFLTSNDFAKGAFLVRDIDLS
                     QILYYVFSAAEEITFVFEGFVGQHFHMLFGVPKEIEGMDSTMDFRPELTVYDPDGEIV
                     EDFVLESIEPEIMYEFFGDTYSYLYVRHDSTLAKKGIYSIKVKAEDPGRAWITFGKKE
                     KFTASQIIAVPRWIREIRSFHYSKGLAKWELYGLGIIGAISAALVLLVFL"
     gene            complement(6993..7589)
                     /locus_tag="PKL93_06615"
     CDS             complement(6993..7589)
                     /locus_tag="PKL93_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNQ70966.1"
                     /translation="MRVKKQQSFHVTRVFLVAFFALSVLLLLFFVFHLFRVKSLEDRI
                     ANLEKHQAALEMEISAVTGVQRTMSINLYYYNELLDRLMNDEVICDTGAVIPVQRTIP
                     YSQSPINDALRLLLRGELTKAEEDLGFKTEFPGRELQFLGARLENGVLFLKFSDPAGF
                     TSGGSCRVNLLKAQIEKTALQFDSVSSVEFEPIDLFQP"
     gene            7675..8571
                     /gene="uvsE"
                     /locus_tag="PKL93_06620"
     CDS             7675..8571
                     /gene="uvsE"
                     /locus_tag="PKL93_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HNQ70967.1"
                     /translation="MKIGYPCINTTLSCTSARTFRLASYSEERLIETVSTNLDCLEEI
                     LEYNRKKDILFFRISSDLIPFASHPICKAKWEDIFRSRFECIGDRIRNYNMRVSMHPD
                     QFVLLNSPRPDVVSNSTAELLYHAKVLNLMKLDGSAKIQIHVGGVYGDKKEASRRFID
                     RYNAVPEAIKERLVIENDERLYSLSDCMAINYETGIPILLDVFHHSILSDGISFADSL
                     SLSGSTWSDEDGIPIVDYSTQQEGARKGRHTDSIDLHNFTSFLEKSRPYNFDIMLEIK
                     DKEKSAEAAIRVTRKDERFQVN"
     gene            8639..10519
                     /locus_tag="PKL93_06625"
     CDS             8639..10519
                     /locus_tag="PKL93_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNQ70968.1"
                     /translation="MKSLTKLSDLSGFKRNLLLLVVDSLIVYFAYILGMYFRYGIFTL
                     DEPEFFVNGIYFSAFIVVSLILNGVYRIAWSYSYFRDYFIILRAVIVGYAIGFLAGRF
                     LNLFEIRFLTVPFTVSTMAAIISVFLLIWSRFFWLSILSRNHPIVSSEERVFIVGAGD
                     AGTSIAEELSRHPENGLVVGFIDDSPKKLRKRIRGIPVLGNTDEIMPLVEKMGVRKVI
                     IAIPSADASTLRRIFSKIDVSKVKAQTLPSITEIMDGKAKLGYLREINIEDLLGRESV
                     QIDLNSLKDYVVGRTVLITGAGGSIGSELCRQIAPLGPGRLLLAGKGENSIYEISQEI
                     GSLFPDLKVSQLIGDVADRSRMRYIFNKMRPQVVFHAAAHKHVPIMEENPTEAFRVNS
                     LGTYNIASLADEFGVETMVVISTDKAVRPSSVMGVSKRIAEEFVRSISSRSNTKFGIV
                     RFGNVLGSRGSVIPLFKKQIQSGGPVTVTDPRMTRYFMTIPEAVTLVLQAGAFSGDGD
                     VFVLDMGEPVKISSLANEMITLAGYIPHQEIEIKYTGVRPGEKLFEELVLTNEELIQT
                     KHPKIFRLKMNEAMDEEALLKTVSRLRTAVEESDFEELNRITSEIVDDSTARIDDGCD
                     SK"
     gene            10516..11055
                     /locus_tag="PKL93_06630"
     CDS             10516..11055
                     /locus_tag="PKL93_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70969.1"
                     /translation="MNWYVDFVRDFPFVSAIIQFSILGTLGDFVSARIARFKTPFPPR
                     IAFMKALEWAFLAIFIKIAFIGYQGFVSALVINGVLPSFFVENLLLNAFARSLSMNLQ
                     FGVFLVLFHRLLDNLVLQTVNWKNMDKALISLIWFWIPAHTLTFALPKDFQIGLAALW
                     SFMLGLLLSLFSRSPQEVK"
     gene            11055..12218
                     /locus_tag="PKL93_06635"
     CDS             11055..12218
                     /locus_tag="PKL93_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNQ70970.1"
                     /translation="MFVPLSRPDITEREISAVTELMTGGILSIGPKVTEFERIFAEYV
                     GVEYAVAVNSGTSALHLVIRSLDLKRGQTVITSPFTFVSSANVALYEGAIPIFADIEE
                     STLNVSPETLEEALANYSRDGLDTGAIKLDPFVPDIFMAVDIFGHPLEWDRIEGICNK
                     HGIKIVEDSCEALGSSFMGRKTGTFGLAGTFAFYPNKQITTGEGGMIVTDDSNIAELS
                     KSMRNQGRGVSENWLEHVRLGYNYRMDEMSAALGVEQMKRIDEILEKRQRVADRYERL
                     LSNISGIETPFVANYATSIGWFVYVIKLDEKIDRESFMKYLTENGVQCRDYFRPIHLQ
                     PFYMQDFGYREGMFPVTERLAKRTVAIPFFNNLSEEEMQFVSYTIEKAVEFAG"
     gene            12215..13114
                     /locus_tag="PKL93_06640"
     CDS             12215..13114
                     /locus_tag="PKL93_06640"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNQ70971.1"
                     /translation="MMSYLYAMILSALLCFIFGKIGMRLGLVDKPSGALKPHERPITF
                     MGGTGIFLAMIPWFFRNPEFFPPILIMWALGFIDDIRGVSPKIRLILEILVGFAVSFI
                     IFGFSTLDSIVLSVVFAGMVNAYNMVDGLDGISSANVIVFAVFAYFTGFVPEIALAIA
                     GVYSGYLIFNFPPARLFMGDQGSYIAGSFVGILLIQSWGTQNFIRIAAICWPVFLDLF
                     VGFLRRSLAKKSPFAGDRDHYYDKIFRLTKNKKRVTLLISTGIALSYAFVGVFIPVIF
                     IPPVLLAASLVQIFFLGSLHSTT"
     gene            complement(13107..14039)
                     /locus_tag="PKL93_06645"
     CDS             complement(13107..14039)
                     /locus_tag="PKL93_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNQ70972.1"
                     /translation="MREILISENGRMNTIDSPVEGCWINVVSPDSGDIAFLKSLEIDE
                     DFVYDALDQEERARFEQDEDLIYVITKLPYLDTADETVPYKTTTLGIAMKSGYFVTIS
                     GSENAIVSDIVEGRVKDISTKKRNRFLLRIFDRATVYYLRYLKEIRRLSNEIESELHR
                     STRNQELVAMLNLEKSLVFFTTSLRSNELMFEKLKRANILTLYEEDEELFEDISIENR
                     QAIEMANIYSDILTGMMDAFASVISNNLNVVMKILTIVTLSLQIPTLVASIYGMNVKL
                     PFMESSTIFYWSMGLSGLAAFLVGFVLFKIRWFK"
     gene            14360..15418
                     /locus_tag="PKL93_06650"
     CDS             14360..15418
                     /locus_tag="PKL93_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ70973.1"
                     /translation="MSALKKYMLLLFLLMIGQVFSVYLNDISRDGVTVSWFTEEPSTS
                     YLIVYDGSNESIHSDEEVTLHRFRVSGLLPGMSYAYTVKSEKDGEVIYKGNGILTTAP
                     NRKTPFSFAAYGDSRNNYVVHSEIVNGIASENVPLVIHTGDIVSTDGAIEEWIDFFEV
                     TEVLSDSVLYSVIGNHESEAINYLKFFAFPGNERYYSLWYGDIFFIFLNTNEAFDKYS
                     QQYVWLLTQLEEAEEEDPAHIVVCLHHPPYSYGSHGDHLYLKEYLVPVFENYNVDLVL
                     SGHDHGYQRIERNGVTYVITAGGGAPLYDITRGESLVASAKAFHYMLFNYTLEGLYGY
                     SKTPEGLILDSFYIPKHN"
     gene            15688..16512
                     /locus_tag="PKL93_06655"
     CDS             15688..16512
                     /locus_tag="PKL93_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ70974.1"
                     /translation="MKNFGLLIVILLLAGTMLSSTLVIKGSNTVYPVAQLWAEGFKAM
                     NPDVEISIEGAGSSTGIKALFNGQTDIANSSRWIKASEIEQMNREGKYFIPYVVAYDG
                     IAIIVNKSLGISNITIQQLFDIYSGKITSWNQIDSSLPKARIVPLSRDNASGTFEYFV
                     EHVMKGEKFAPQVQQLASTRAEVEQVMQNQYAIGYIGMGYITEEVKALTVEGVEPTVA
                     NVNAGSYPISRPLFMFVDATNGLPTGVTGEFLRYALSPEGQAAVLSVGYVNAYGTK"
     gene            16528..17406
                     /gene="pstC"
                     /locus_tag="PKL93_06660"
     CDS             16528..17406
                     /gene="pstC"
                     /locus_tag="PKL93_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNQ70975.1"
                     /translation="MRRRRLDLSSRVLVTTAALITSAILFGIFFFLISDGVRVFGKIS
                     FEEFFFSARWYPTYEDAEYGILALLSGTLAVTGLTLAISIPLGFVSAIFLAEFSHRKA
                     HDFLKLIFELMAGIPSVVLGSFGLKYVSRWLMEFFPMKVWTGLNIFNASLMLSILAMP
                     YFVTLIEDALKAVPVDQKEASLALGANTTSTVFRIIVPQARSGILNAIILGTNRIIGE
                     TMVVLMVAGGASMIPHSIFDPVRPLTAAIAGEMGEVELGSTHYYALFMIGVVLLGISL
                     IFTAIAMRLKRGMRRC"
     gene            17400..18236
                     /gene="pstA"
                     /locus_tag="PKL93_06665"
     CDS             17400..18236
                     /gene="pstA"
                     /locus_tag="PKL93_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNQ70976.1"
                     /translation="MLIDRITKLIFGIISYTLVAVILAIIGFLVVNGLDEISLEFLVS
                     FPKNSMTEGGIWPSILGTFYFLSIALLFSVPVGILSAVYLSEYSKENRFQRLVLTANN
                     ILAGIPSVVFGMFGLSLFSITFGFGTSIISGGLTLGIMSLPYIISNTHESLDAVPKSY
                     REASMALGANKAETTFRIVVPASFSRILTGVMIAVGRIIGETAPLLFTGAAFYITRNP
                     SGLFEPAMSLPTHIFVLSAIYPENVTPKLEGSISVLLIIVITLFVSVAIRRRIESKKL
                     EE"
     gene            18238..18990
                     /gene="pstB"
                     /locus_tag="PKL93_06670"
     CDS             18238..18990
                     /gene="pstB"
                     /locus_tag="PKL93_06670"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNQ70977.1"
                     /translation="MEEPIFKIEKLNVYYGDKHALKDVTCEIPKKKVTAVVGPSGCGK
                     STFLRVLNRMNDLIPSYRHTGSVKLLGNEILNMDPVILRKHVGMVFQKPNPFPKTIQE
                     NVTYGPRIHGIKNRKTLREITEKSLKQAALWDEVKDELKKNATNLSGGQQQRLCIARA
                     LSVEPDVLLLDEPTSALDPIATQRIETLIEELSDKYTIIIVTHNLQQALRISDYVMFF
                     YVGDLVEFDTTEKITSNPRDQRTSEYLRGIFS"
     gene            19006..19683
                     /gene="phoU"
                     /locus_tag="PKL93_06675"
     CDS             19006..19683
                     /gene="phoU"
                     /locus_tag="PKL93_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNQ70978.1"
                     /translation="MTPERQRHLEAGFNRLKSMLSDMMENVRSSLLKAIDSLDRLDGD
                     LARVVIENDEVIDKLQREMDTEICNYIGRFQPLAEDLRYVLTMMKLATDLERIGDLAV
                     NIAEVAVRYENQTLVKPLIHIKKMTAVVEKMLEEIIDAYYNKDTETAKRVWNEDNNVD
                     EYYLYIKREVREKLVSMTDPNAIGQLLDLMLVSRFLERAGDHATNIAEEIYYIEKGES
                     LKEEMRR"
     gene            19680..20354
                     /locus_tag="PKL93_06680"
     CDS             19680..20354
                     /locus_tag="PKL93_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ70979.1"
                     /translation="MKVLIVDDDLDILNIVRTACENESMQTSTAANAEELWSSLRSEK
                     PDVILLDIMLPDANGLDLVKKIRTMYSRIPIIFLTARKSDLDMILGLELGADDYVTKP
                     FNPRALVARIKAVMRRTEISGTLEDKLTIGDAVVNLKSYTVTKNGKSEELTRREFELL
                     KLLAENPGRVFTREELLDKVWGMDFFGDFRTVDVHISKLREKVGEGFIKTVRGVGYKF
                     VIGEQR"
     gene            20351..21328
                     /locus_tag="PKL93_06685"
     CDS             20351..21328
                     /locus_tag="PKL93_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ70980.1"
                     /translation="MNESLLDELNEAIIMVDEGLVVTRANLSARKMLGDVEGLALPDA
                     LHVQGISKIVDNLISGTNYTIDTIFVKDDTTHYLKIRVSAPYIIARNITSEKLFESAK
                     MDFVNSIVHEFSTPLAVINGYVQLLIEKKMELPADVSETIERIARSTGRLSRLVEELG
                     ILSNLELQNYRVKIETVNLKELIDESVSDLESKWKRKKLKITTTVSPNTYAAVDSLLL
                     FRVISNLISNAVKYSSVGDTIEIVSREVESRIYISVKDNGIGIKSEELPRIFERFYRG
                     SNARTSGSSGLGLGLSLVKHALNLINGSIEVRSRYMMGTTVTISFPKNM"
     gene            21511..22695
                     /locus_tag="PKL93_06690"
     CDS             21511..22695
                     /locus_tag="PKL93_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HNQ70981.1"
                     /translation="MKSDTHSVCLYGSAHDLRKAINTFLKEGLSKNYKLLYFGPERDV
                     IPSKETTDGLPYNLDDLLGSKTLEIIDFEKSYVKNGVFDPVRQISFLQEAAVRAIKEG
                     YNGLGVAGEGTAILESGTKMEELLKYEASVSDLVEESPVTAYCLYDSSRIDQSSALEI
                     SRLHPYTLRESGFSVNEDYYRAPRRTLEYDTASRLDICRGTANSLLQMFRMKSIYVEE
                     HEENVSKLASAIAKEMALDSFTMACLEMAGKIHDLGMNSMFLDMLTRPGPLHALEARL
                     LRRHPITGFEISRGVPFPDKVSYAVFQHHERIDGSGYPNGLKDSEISLEGSILAVSEV
                     VAAMSFYRPYRKPYGQEYSLDQIVKYKGSLYRTEVVEACLTAFENGFSFESSLKTSAS
                     QP"
     gene            complement(22826..23647)
                     /locus_tag="PKL93_06695"
     CDS             complement(22826..23647)
                     /locus_tag="PKL93_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNQ70982.1"
                     /translation="MGEEFRITEKLKAMIKGLNPAEKKAARYIIDFPDDVIHHSISEL
                     SVLAGTSETTIFRLSKKLNFNGYQALKIQLARELSRESPPISADKNKLISDLIEALNQ
                     QNQLIDDVQLDRIAGKIVRANRLIFFGVASSGIVAEFGATLFMRAGFSTAFYTDPHLQ
                     IMTAVSLNRGDIVFGISASGNIRDTVKSIEVASDSGAYTVAVTGGIGSRIVSVSTETI
                     YVAQGGHETGIPLLQPRVCQLTVIQLLLEKSIKLRNGTLQTLEKVDRTLDKKRYL"
     gene            complement(23626..24825)
                     /locus_tag="PKL93_06700"
     CDS             complement(23626..24825)
                     /locus_tag="PKL93_06700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ70983.1"
                     /translation="MPGLSHDSYDIVVGIPSFNNETTIKYVVETAAIGLKQYFPSMRG
                     CIVNADGGSTDRTKEMFYSASSNGVDLLSFVYEGISGKGSAMRSIMEKSKSYGAQAVL
                     FLDSDLRSVEPFWIERLASPILEEGAAYVTPYYIRHKYDGTITNSICYPLTTALYGKK
                     IRQPIGGDFGVGRELLEYYTTVPENTWRSDISRFGIDIWMTTSAVNEVKGSIYQAALG
                     TKIHDVKDPGKQLGNMFKEVVGTLFSLMQKYEDNWKTVGDYSDSPIYGDVVTGIPEPL
                     EVDLENMISKCYEGIHSQEDFIKDILGAKIARNLLLLGKSDAMIDDDLWVRIIYRIAS
                     GYRKLALRDRLIELLVPLYFGRVASFVSRTEKMTQEGAEEEIDKLLKKFVETKDELIS
                     IWEKSSE"
     gene            complement(24837..26132)
                     /locus_tag="PKL93_06705"
     CDS             complement(24837..26132)
                     /locus_tag="PKL93_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ70984.1"
                     /translation="MVKRKSPFKRGMFIFGILLLCFFILIPFYFMIHVSLKADYDPNK
                     LTFSNFTVRNYLEIFGLIQSSETEFFGDEIIRANLEPVLKRIEELEERTASLESYTRY
                     VHEIQLEAKEKELRDIVDLVIDFSGIEVQNREEFIERAITPGTEETEIVEENMKSIFS
                     ANDVKKFIDLREEVDSALTEESLLAGLENAKAQLKSYEAQKKKIIEERASEFPFLRYL
                     SNSLVFAGISALISLIVAIFGAYAFSRLRFKGRSSVQRSVLFVYLFGGTVIMVPLYQM
                     AVKLGILATPLGTGIYLIMVYVIQTLPVSLYMLGNYFRTIPFSIEEAAIIDGCSRIQA
                     IFKIVIPLSLPAIVTVYIYAFMIGWNEYLFASAFLGLKSYKDLFTLPIGLNAFSGSAH
                     AVWGRLMAASVVSAVPIIIIFSLMQKYLTSGFTAGGVKE"
     gene            complement(26126..27001)
                     /locus_tag="PKL93_06710"
     CDS             complement(26126..27001)
                     /locus_tag="PKL93_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ70985.1"
                     /translation="MQRAASSMLKKENSLGWKLVSPTVILLMILILYPVGYNIYISFF
                     DYGITSSTFVGLENYVRVFKDSAFWKSFFITVGFTLMTVIGSILLGLVVALMLNKEFK
                     GRAIVRTIVLLPYITPLISIVFSWKYIFDPSYGPFMQLFSQTLGWVSPQLDLLNNSNN
                     AFLVASVFNIWRNFPFVYLMLLSRLQSIPDDYYEAAEIDGATSWKQFTNITLPELYFV
                     MGAVALMRGIWNFYKFDEVYLISKRAGTLPIFIYERVIGTTSPEYGVAAAIATILMVI
                     MLFLITVYVKKVLKW"
     gene            complement(27058..28353)
                     /locus_tag="PKL93_06715"
     CDS             complement(27058..28353)
                     /locus_tag="PKL93_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ70986.1"
                     /translation="MKKIFFVVFLVLMLSVTMTAKLVYWHTQEEESRQQVIAQIVESF
                     TIETGIEVDVVAIEENEMFSRLAAASAAETLPDLIEAGAETILGLGADGLLDTAIPTR
                     FIKNGGDFYNGATRFVITPAGDEYFAVPYHGWVQGIWYRKDWFEKAGLAEPTTWEAIM
                     EAAKYFHDPQNGMYGIVLGKANDAYAEQVFTIFALSNGARIFDKEGNVIVNSPEMKEV
                     LQFYKDLGEYSAPGHTYWQQARELFLAGKTPMMFYSTYIMDDLALAEIQTQYISNFDP
                     ELVENTEFAPMMTHSRSSSFGQVLSLAVIKSSENKLDVLKFLDFVFEPDNYIKYIHMA
                     PGGMLPTRSSIAESEEFLNDPKGIYRRYGAEKIKSIIYGMENIEKFGYVEGRVFPEMG
                     KISGAFTIGNGIVMMFDNDATPDQVLTFWREDIRKLIGR"
     gene            complement(28636..29277)
                     /locus_tag="PKL93_06720"
     CDS             complement(28636..29277)
                     /locus_tag="PKL93_06720"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNQ70987.1"
                     /translation="MEKRIPLIGEDFPEMKVKTTHGVLNLPEEYKGKWFVLFSHPGDF
                     TPVCTTEFVGFQKRYQKFRELNCELIGLSVDQVFSHIKWAEWIEETLGVKIEFPIIAD
                     EMGEVGMTLGLIHPAKGTNTVRAVFIVDPKGKIRAILYYPQELGRNMDEILRMVKGFQ
                     VADKNGVAIPADWPNNEIIGDHVIIPPAADVKTAAERKGKEGCYDWWFCHKSL"
     gene            complement(29392..29715)
                     /locus_tag="PKL93_06725"
     CDS             complement(29392..29715)
                     /locus_tag="PKL93_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70988.1"
                     /translation="MYIVCDLTYAVSESRSTEALKMILETLSKKLLLGAQGDTLNMRA
                     ETEDTIEVQIMALKGSCSFEEVVPRINEYLSLISRALETSFPDYRVVSSYIYLDGEET
                     PYLLC"
     gene            30140..30961
                     /locus_tag="PKL93_06730"
     CDS             30140..30961
                     /locus_tag="PKL93_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ70989.1"
                     /translation="MIYRTLGKNMGEIPAIGIGTWEIGGRDLPDLSRDKEHIDTISRA
                     IEMGYTHIDTAEYYGGGHTEEIIGEAISVFSREELFITSKVWPTHLWKEELHRALEGT
                     LRRLRTDYLDLYLIHWPNPDVPLEETLNAMSEEVVNGRVKFIGVSNFDTPLLKKAVAV
                     SKQPIVNNQVLFNIDDRQPMEELLPYCQSHEITLTAYSPLKRNSLKPSTEKLLKDFAV
                     KYSATVQQIMLAWLLGKEGVVVIPKASKLDHLRLNLEAGSIVLDDDDTRRLDSLE"
     gene            30972..31625
                     /locus_tag="PKL93_06735"
     CDS             30972..31625
                     /locus_tag="PKL93_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488063.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNQ70990.1"
                     /translation="MNEIRSSFIEALDSQDKKRAVEICIDALDSGSIEVVDLYLEILG
                     PALGNWECDYESDRMCIWNEHIRSSIVRTVMECCYPYIIGKRGGKRSAEKEVTVAVLC
                     PPEEYHEIGARMISDIFTIAGYNSTFVGANTPLDTFLEAMKELNPSYIAISVSNYYNL
                     INTRRMITKIREHGGKSRIVVGGKAFEKNPNYWKEVGADIFLKDPREIFSFGEGDKK"
     gene            31622..>32178
                     /locus_tag="PKL93_06740"
     CDS             31622..>32178
                     /locus_tag="PKL93_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ70991.1"
                     /translation="MKLAFSIAMRFLSSSKVQTLLIVLGIAIGVSVQVFIGSLIQGLQ
                     KDLVDTTIGSSSQVTITSSEEDNRVADWESIISEIDSLDLPTELTALSASADLPVFID
                     SGDRTLSVLLRGLQFPESNAIYKIDTRLVDGKIPENYREILIGKGLKEDLEVKIGEKI
                     TIFTPDRTVETLEVVGIFDLGVASL"
BASE COUNT         9306 a         6863 c         6947 g         9062 t
ORIGIN      
        1 gaagaagaat aggtgtaata attgagttgt gaagggagat gaccttgaat ggaactggaa
       61 agaattctcg tagttgatcc aatagatggt gaatacacag cagatgttag gatttctgga
      121 agcaaaatcg aatcaatcaa gaagaccgga ggagatttca gatcgatagc tatgcccggc
      181 ttcgttgaca ctcattcgca tggcgccctt ggaataaact gcatgacaat gaaccatgaa
      241 gggttggaaa agtgggagga atttgctcaa tctcatggcg tgacttacct tctcccaacc
      301 acagtatctg ctagcgagga aggaatgaag agagttgcag atctagtatc taaatacatt
      361 tccgacaggt caagaacggt ggttcacgga gtgcattttg aaggtccata tattaacccc
      421 aagaagaaag gtgctcaaaa tccggaagca attaggcagc caacactatc agagataaat
      481 tctgtgcttt cagatgtagt tatgataatt acgatggccc ccgaggttgg aggatttttg
      541 gaggtccttc ccgagataat aaagcgagaa atcgctgtct cgatcggtca tacagacgcc
      601 acttataacc agatcaaggc cgcttataag ttaggtgtca ggcggatgac tcacttcccc
      661 aacggtatga acacccttca ccacagagag attggttgtg ttggagcggg gttcctgctt
      721 cctttgaatc tcgaaatgat tgtcgacgga gttcatacca cacctgaatt cgtagaaatg
      781 gtctacaaaa tccgtggaag cgaagcgata attcttgtta ccgattctct tgatgcaacg
      841 ggtttggata atgggattta cgatctcggt ggtctaaggg tagaagtaaa agacaatgtt
      901 gcaacacttg aagacgggac tctagctgga agtactctga caatggacaa agcaataaga
      961 aatttcaaga ggtggactgg ctgcaatttg caagaactgg cgaaagtatc ttcctacaac
     1021 gctcttaaga atcttggcat ctccaacagg ggaagattca aagagggctt gattgccgat
     1081 attgtgttgc ttagcaaaga actgaaggtc gaagaaacga ttctagctgg caaatctgtg
     1141 ttcatttcca agtagctatg agatcgcgaa aaccagagtg ggtagctttt cccaaaagaa
     1201 aggctttctg attttttgga gatttttaag aagaagatta tagtgacttt tctcgtcgaa
     1261 cgtgcattgt attgagtaga tttaattatg atgcatcacg cattcccaaa ctaatccact
     1321 aggatctggt gctgtagtga cagagtcact tcaccagaat ttacgagctt accctattca
     1381 ctaatccgga ctggccacag caattctcct attgtctgca agctttttct aagataagtt
     1441 aacaacgaac attgtgtggt aagaatgata catatcctct tatttgggag aatggactga
     1501 tttcttcaag aagactcacg actattgttt atgcagagaa ttctctatca ataatgatat
     1561 gctgtaggtt gaagtctgat aaggaggtgt gaaaaatgga gcttcataaa tcaaccatcg
     1621 cttattcact caaaagggag ctggactacc ttctctcact ccccttacaa tacgacgaag
     1681 aagatactga atggccactt attctatttc tccatggagc gggtgagagg ggagacaatt
     1741 tggagctact gaagaagcac ggaattccac gagtggttag cgaaatgagc gattttcctt
     1801 tcataaccgt ctcgccacaa tgtccggaaa atgattggtg gctgaacaga cttcaggacc
     1861 tcagatttct cctagaatcg atcattaagc agtacagagt cgatcactca gcaatctatc
     1921 tcacaggcct ttctatggga ggtttcggaa cctggcatat ggcagttgag tacccagatt
     1981 tattttccgc catagctcca atttgtggcg gaggattagg gattttggga ttcccggaaa
     2041 gagtgatcga tatcaaggat ttgccggttt gggcctttca cggagaaaaa gacaatattg
     2101 tctctgttga agagagcaga attcttgtga aaactttgaa ggaagccgga ggaaacgcaa
     2161 tgctaaagat ctatcccgat gcaggccatg attcatggac agagacttac tcggatccgg
     2221 aactttatga atggttcatg agcaagcgta aactaggttg acttcgttct tatggaactt
     2281 acctgagatc tgttgagaat cattctggat ggtttgagat ttccagttct aaaacctctc
     2341 ctgctcctga caatctgttt acgagcctca aaatagactc ttagtgaaag aacccagatc
     2401 actgctacca atatccaaac cgctatcaac attatcagga gccacccaaa cccttcaagt
     2461 ctttctcttg ctccaaagaa tctcgtgatt ccataaatcg atacgggagt tactattccg
     2521 agtaaaaccg atgctcttat gaagttgttt cttatgagtc ctattttggg ccttctgctt
     2581 ctccttactg caaaacccat taaatcacca cctcaagtta cttcttgtct gagtaatgta
     2641 attatacact cattgttttg cctgcccttt aatagagaaa tactttcgcc gaaaacttcc
     2701 aacataagac atatgagatc catttctcat aagcaacaac gaatctctct ttcttccaga
     2761 agaatttggc agtcgctctt ttaatttctc gcctctcctc acttgtaata gagtattctg
     2821 tgaattcaaa ttgtggaggc tcaaatggtc aagttaacag atgaatcatc gaaagaggct
     2881 tttatcgatt actgcaggaa attcatccgg agcatggcga ttcttttttt tgctatcgga
     2941 gacatttgtc gccgctgaag atcatccttc agtgattctc ttaaacagca acgatgaaat
     3001 tatgggagct gcctctctaa ttcttgaggg aaggtatagg agtttaagaa agggaagatt
     3061 catgattctt cattccagag catcaatgac ggatgcctat agatctcctt acaccattac
     3121 taagaaaaat ttctggaata gacgaggtct atctctttgt ttcatcagaa aaaccaatcg
     3181 aatccattct aaaggaaatc ggtttcaaag ttgagagata ttccttcgta aagagaaggc
     3241 taattagcag ctctctagaa accccccgag gaaatcgatt tcacatttga actggagaaa
     3301 catggagaga ctttctccaa aattataaac tctgctttcg acgaaatagc aagccatttc
     3361 gacaatgacc cgggctagtt cagaggtaat ttgtccaaca gcacagttcc agattatgga
     3421 attcagcttc tttatgaaga aaatgaaact gtaggtctct tcttttcgga aaccagtagc
     3481 gaaagactcc ttgaaatagg tcctctggcg ttactgccag gcttttaagg gcaggatttt
     3541 agaaagctct tgttaagaaa aacgttggct ctttattatg aattgggcct tggaagtgtc
     3601 ctcttggaga atacatgaaa tgaaaacaat ctttctcttt atcttgaaga tggatttgag
     3661 aaagtacagg caaaaacctg ttacaacctg accgtctaga tcactgctgt ctagatcgct
     3721 ccagatttct tataaactct agcacttctt cggcatgacc ctttgcttta acgtttcgat
     3781 attcttttgc gagaattcta ttttcattaa ttacaaaagt cgatctttct gttcccatat
     3841 attcacgacc gtacatcttt ttcggtttga tcacgtcgaa catctcgtga atccttccat
     3901 cctcatcact cagaatagaa aagttcaaat caagcttcaa cgagaagctc tgatgagatt
     3961 tcagactatc tttgctaaca ccaattatga ctgtatttag accggcgaac tcgctcgcca
     4021 aatctctaaa gccttctgct tcgagcgtac aaccggaagt gttatctttt gggtaaaaat
     4081 agagcacaat cttcttcccg gaaaagtggg acaacgatac ctcattgccg ttttgatcaa
     4141 gtaatgaaaa gtccggtaca ttcttgccca gagaaatctc ggccattatt tcacctccac
     4201 ctttttcgta tgtcgggagc gccccatcag gatcaagaaa ggagccccgt ttcactcatc
     4261 ttgattatag ggcaaattac tctaagcact tagaattctc agccctgtcc tctttgcttg
     4321 caaaacgaca gtgctgtgct cattagaaat tagactcccg gaaaattgca ggtacaacct
     4381 actctaagcg tatcgaagtc tttttctcta ctggtgttgt cagattatct tctgaagaaa
     4441 gattcatggc atggatttgc acatattcta gcagtggaga aaattccacc ccttatatct
     4501 taagagaata gcggcttcca ggcggaggcc gccttttttt cgtagccctt cttgctgata
     4561 taatcatttt gagatctctc tttgattacg aagataaggg gtgaaaggaa tggagttttt
     4621 ggcggctcaa cttcctgaga agatcggctt gcttgagagc aatggagact ataaaggggc
     4681 tataaaagaa atcgataatg agcttgaaag ctatcttcct tctattatga caagaagact
     4741 gttatgggaa aaggaaagaa ttctcagact gaaggacaat tatccataca ctaattcaca
     4801 ggcatttgag attctatcga attctataga caacttcacc agagaggaat ttgaaggttt
     4861 atgtgaagcg aagcaactag actccatgac tctcgacggt gaggcaagat tcgaaagaag
     4921 attcgataag aatcttctat tcgttatgcc ggaatatggt agtcgactga agcaaaaaga
     4981 taacgaacga gacgaaataa gggtgctcct ccacagagaa atagacaggc ttatcagcac
     5041 tagagaaccc tcaaaattca aagtgaatgc tagatcgtca atcaatgttc aaagctctga
     5101 ggaaaatgtt tttcgctgtt ggttgccagt atccagaaat ggagaacaag tttcctcaac
     5161 gaagatcctc aacacaagtc acagttattt cctgtctccg gcagattact cccaacgcac
     5221 cgtatacatg gagtcagatg caaaaaaaga taccgttttc agcgtcgaat ttgagtacga
     5281 aatctccgaa atatcgactt ccctcgatcc ttccagatgc acacatcccg atgaaaagag
     5341 atttgaaagc tatttgattg aacgagcgcc ccatatcgta ttttcaccat ttatgagatc
     5401 tctagccgat gaaatctctg gcaaagagac caatccctac tacatcgcta agagtttcta
     5461 tgactggata tgcgagcatg taaaatatac attcatgagt gaatatgccc tttatccaaa
     5521 tctttcggaa ttcgccgcta ccaatctcag gggtgactgt ggtgtaaaag ctctgctgtt
     5581 tataacactt tgcagaataa agggaatacc cgctagatgg caatcaggat ggttcgcatc
     5641 tccaaaaggt gcgagccctc acgactgggc tttgttctgg ctcgaacctt ttgggtgggt
     5701 tccagccgac tgttccttcg gaggatcaag aaaggatatc cctgcttata gagagttcta
     5761 cttcgggaat ctagatgcgt acagaatgat ctcgaattca gaatttatgg cacccctgtt
     5821 gccggccaag agatttttca gatctgatcc atatgacaat caggtaggag aaatcgagac
     5881 agaaaagaga gccttgagat cgtacgagag ggagtgctcc attgaaatac tctcttttga
     5941 aaggttaacc taactaaagc tgaaaacgga gatcactctg aaagatcttt tttcctctcg
     6001 ctgttatcag tttatgaaat cgccaaaaca atcttttcgt cagcccttga aaggataaaa
     6061 tgctgcgatt attcgatagc tatcgaaaac cgatcatttt gaactgacgc aaatctgttt
     6121 ttctgtccat atgccttcag catgagagaa aatgccttga atgctgatag acttattaaa
     6181 atagattact acataggagg cattccatga agaaaaaatt cgtcctgctt tctttgttgg
     6241 ttatttttgc agtttcagca ttcgcccatg taccggcctt cctaacatcc aatgactttg
     6301 ctaaaggtgc attccttgta agggacatag atctgtcaca gatcttgtat tatgtcttct
     6361 ccgcagcaga ggaaatcaca ttcgtattcg aaggttttgt cggtcagcac ttccacatgc
     6421 tttttggagt accaaaggaa attgaaggca tggattccac aatggacttt cgtccagaac
     6481 tcacagtata tgatcctgat ggagaaattg tcgaagattt cgttcttgaa agtatagaac
     6541 ccgaaataat gtatgagttc ttcggtgata cctattcata cctatacgta aggcatgact
     6601 cgacactagc taagaaaggg atttattcta tcaaagtgaa ggctgaagat ccaggaagag
     6661 catggataac gtttggaaag aaggaaaaat ttaccgcttc ccagataatt gcggtaccac
     6721 gatggataag agagatcaga tcatttcact attcaaaggg actggccaag tgggaattgt
     6781 acgggctggg tatcatagga gcaatatctg ccgctctggt gttgttagtt ttcctctgaa
     6841 aaaagaagct caagattagt aatcgtgtaa ggtaaaaaga aaaacgccca tttcggacgt
     6901 cttaattaat attataccac gagattttgt taaagtcaac tattttgcaa attgcaacag
     6961 tgtaaattac tattcaaaga tcaaatctaa gatcagggtt gaaagagatc gattggctcg
     7021 aattcaacgc tgctcaccga gtcgaattga agagctgtct tttcaatctg agctttaagg
     7081 agatttaccc tgcaagaccc gcccgaagta aacccggcgg gatctgaaaa cttcaagaac
     7141 aaaactccgt tctcaagtct cgctccaaga aattgcagtt ctctgccggg gaattctgtc
     7201 ttgaaaccca ggtcttcttc ggccttagta agctctcccc ttaacaacaa acgcagcgca
     7261 tcgttaattg gtgactgaga atatggaatc gttctctgga caggtattac agctcccgta
     7321 tcacagatga cttcgtcatt catcagtcta tcgagaagct cgttataata atagagattg
     7381 atactcattg ttcgctgaac accggttacc gcactaatct ccatttcgag ggcagcctga
     7441 tgcttctcga ggtttgctat cctgtcctca aggcttttca ccctgaaaag atgaaagaca
     7501 aaaaacagta gcagcagaac tgaaagtgca aaaaaggcaa caaggaaaac tctggtgacg
     7561 tggaaagact gttgcttctt aactctcaaa tacctcacct ctcaatcagt atcgaatcaa
     7621 ttatacattc ttcgcctgaa actccgctaa aatactatta ttaggaggtt atgtatgaag
     7681 atagggtatc cttgcataaa cacaactctc agctgcacct ctgcgagaac tttcagactg
     7741 gcatcataca gtgaagagag actgattgag actgtctcta ccaaccttga ttgccttgaa
     7801 gagatactag aatacaacag aaaaaaagac attctcttct ttaggatttc atcggatctg
     7861 attccatttg cttctcaccc aatctgcaaa gcaaagtggg aagatatttt tcgaagtaga
     7921 ttcgagtgca taggcgatag gataagaaat tacaatatga gagtatcgat gcatcccgat
     7981 cagttcgtcc tgctcaattc tcccagacct gatgttgtaa gcaacagcac cgctgaacta
     8041 ctttaccacg caaaagtcct caatctcatg aagctagacg gcagcgcaaa aatccagatt
     8101 cacgttggcg gagtttacgg agataagaag gaagcctcca ggagattcat agacaggtat
     8161 aatgcagttc ctgaagctat aaaggagcga ctcgtaatcg aaaacgacga aagattgtac
     8221 tcgctttcgg actgtatggc gattaattat gagacaggta ttccgattct ccttgatgta
     8281 tttcatcatt caattctttc agatggaatt tcatttgctg attcccttag tttatccgga
     8341 tcaacgtggt cagatgaaga tggaattcca attgttgatt acagcactca acaagaaggc
     8401 gcgagaaagg gaagacatac agatagcatc gatctacaca atttcacttc ttttctagaa
     8461 aaaagtcgcc cctacaattt cgacatcatg cttgagataa aagacaagga aaagtcggca
     8521 gaagcagcta tccgtgttac gcgcaaagac gaaaggttcc aggttaactg aatccagtgt
     8581 aaaagaaaga tggaggcgaa tttttacttc gagtctattg tgctagaatc tccatgaggt
     8641 gaagagtttg acaaaactca gtgatctttc cggcttcaag agaaatcttt tgcttctagt
     8701 agttgattca ttgatagtat attttgcata catattaggt atgtacttca gatacggtat
     8761 tttcactctg gatgaaccgg agtttttcgt taatggaatt tatttcagcg ctttcattgt
     8821 cgtttctctt atactaaacg gtgtatatag aatcgcctgg agttacagtt acttcagaga
     8881 ttatttcata atactccgag ctgttatcgt tggctatgca atcggatttc ttgcaggtcg
     8941 atttctcaac ctctttgaaa taagatttct cacggtgcct ttcacagtct cgacgatggc
     9001 agcgattatc tccgtttttc tcttaatatg gtcgaggttt ttttggctca gtatcttgag
     9061 tagaaatcat ccgattgtct cttccgaaga aagggtcttc atagtagggg caggagatgc
     9121 cggtacctca attgcggagg aactgtcgcg ccatcctgaa aatggattgg tagtgggttt
     9181 tatagatgac tctccgaaaa agcttcgaaa acgaattcgt ggaattcccg ttttggggaa
     9241 tactgatgag attatgccgc tcgtagagaa aatgggagtc cgaaaggtaa tcattgctat
     9301 cccctctgcc gatgcaagca cattgagaag aatcttttcg aagatcgatg taagtaaggt
     9361 caaggctcag actcttccaa gcattacgga gataatggac ggaaaggcga aacttggata
     9421 tctgagagag ataaacatcg aagacctgct tggaagagaa agcgttcaga tagatctgaa
     9481 ctcactcaag gattatgttg ttggaagaac tgtgctcata accggtgctg gaggaagcat
     9541 tgggagtgag ttgtgccgtc agatagcccc tcttggacca ggcaggctct tgctcgccgg
     9601 aaagggggaa aacagcatct atgaaatcag ccaggagatt ggctctctgt ttcctgatct
     9661 caaggtcagt caactaattg gcgacgtggc cgatcggtcg agaatgagat acattttcaa
     9721 taagatgagg ccacaggtcg tcttccatgc cgctgcccac aagcacgttc ccataatgga
     9781 agagaaccca acagaagcat ttagagtcaa ctcacttggc acttacaata ttgcaagtct
     9841 ggccgatgaa tttggagtcg aaacaatggt cgttatttcg acagacaagg cagtcaggcc
     9901 aagttctgtc atgggtgttt caaagagaat cgcagaggaa ttcgtgagat ccatttcatc
     9961 gagaagcaat acgaaattcg gaattgtcag gtttgggaac gttcttggaa gccgtggaag
    10021 tgtgatacct cttttcaaga aacagatcca gtccggtggg cccgttaccg ttacagatcc
    10081 cagaatgaca agatacttca tgacaattcc ggaagcggtg acgttagttc ttcaagcggg
    10141 ggcattttcc ggagatggag acgtctttgt gcttgacatg ggggagcctg tcaaaatctc
    10201 atccctcgca aacgagatga ttacccttgc cggatatatt ccccatcagg aaattgaaat
    10261 aaagtacaca ggtgtcagac cgggcgagaa gctcttcgaa gaattagtcc tcacaaatga
    10321 agaattaatt cagactaaac atccaaagat cttccgtctc aagatgaatg aagcgatgga
    10381 tgaagaggct ctgcttaaga cagtttccag actgaggacc gcagttgaag agagcgactt
    10441 tgaggaattg aacagaataa cttctgaaat agtcgatgat tcaactgcca gaatagatga
    10501 tggatgtgat tcaaagtgaa ctggtatgtc gattttgtta gagatttccc tttcgtctca
    10561 gcaatcattc agttctctat tcttggaact cttggcgatt ttgtcagtgc gaggattgcc
    10621 cgcttcaaga cgccgtttcc gccaaggatt gctttcatga aggcccttga atgggctttc
    10681 ctagccatct tcataaagat cgcattcatt ggttaccagg gcttcgtttc tgctcttgta
    10741 atcaatggag ttctaccgag ttttttcgtc gagaacctct tgctcaatgc ctttgcacgc
    10801 tcactttcga tgaatcttca gtttggtgta tttcttgtac tttttcatag gctgctcgac
    10861 aatttagtac ttcaaacagt caactggaag aacatggaca aagcgcttat ttctttgatt
    10921 tggttctgga ttccagctca cacgcttacg tttgctttgc caaaggattt ccagataggt
    10981 ctcgctgcac tttggtcatt tatgttagga cttctgctct ctttattttc aagatctcct
    11041 caggaggtga aataatgttc gttccccttt caagacctga cattacagaa agagaaattt
    11101 cggcagttac ggagttgatg acgggaggca ttttatctat tggccccaag gttacagagt
    11161 tcgaaagaat cttcgcagaa tatgtcggtg ttgaatatgc cgtcgcagta aacagcggaa
    11221 ccagcgccct tcacctggtg attcgttctc tggacctgaa gaggggtcaa acagttatca
    11281 caagcccttt cacttttgta tcctccgcaa atgttgcttt atacgaagga gccatcccga
    11341 tattcgcaga tattgaagag tcgacactta acgtatctcc ggaaactctg gaagaggccc
    11401 tggccaacta ctcaagagat ggtctggata caggtgctat caaactagac ccatttgttc
    11461 cggacatatt tatggcagta gacatctttg gccatccact tgagtgggac agaatcgagg
    11521 ggatctgcaa caaacacggg ataaaaatcg ttgaagactc gtgtgaggca ctggggagct
    11581 ctttcatggg acgaaagaca gggactttcg gtcttgccgg cacatttgcg ttttatccaa
    11641 acaaacagat tacaactgga gaaggcggaa tgattgttac cgatgacagc aatattgcag
    11701 aactttctaa aagcatgaga aatcaaggaa gaggagtctc cgaaaactgg cttgaacatg
    11761 taagacttgg ctataattac aggatggatg aaatgtcagc cgcacttggc gtggaacaaa
    11821 tgaagagaat tgatgagatt cttgaaaaac gacagagagt tgctgaccgt tacgaaaggc
    11881 ttttgtcaaa catcagtgga atagaaactc ctttcgttgc aaattatgct acgagcatag
    11941 gctggtttgt gtatgttata aagttagacg agaagataga tagagagagc ttcatgaaat
    12001 atctcacaga gaatggagtc cagtgccggg attacttcag acccatacat cttcagccat
    12061 tttatatgca agacttcgga tacagagagg gtatgtttcc ggtaactgaa aggttagcca
    12121 agagaacggt cgcaattcct ttcttcaaca atcttagtga agaagagatg cagtttgtct
    12181 cttacactat tgaaaaagcc gtggagttcg ctggatgatg tcgtacctct atgccatgat
    12241 tctttcggca ctattgtgtt tcatttttgg taagatcggt atgagacttg ggctcgtgga
    12301 taagccttct ggtgctctaa aacctcatga aaggccaatt actttcatgg gaggaacggg
    12361 gatatttctt gccatgattc cttggttttt tcggaatcca gagttctttc cgccaatact
    12421 gataatgtgg gctttgggct ttattgacga cataagaggt gtttccccga aaatcagatt
    12481 aatattggag attcttgtgg gatttgcagt gtcttttatc atctttgggt tttctacatt
    12541 agattcgatc gttctctcgg tggtttttgc aggcatggtt aatgcttaca acatggtaga
    12601 tggtcttgat ggaatttctt cggcaaatgt tatcgtattt gcagtgtttg catatttcac
    12661 gggattcgtt cctgagatag ccctcgcaat tgcgggcgtt tactccggat atctaatctt
    12721 taactttcca cctgccagac ttttcatggg agatcaggga tcgtatatcg caggttcctt
    12781 tgtcggaata ttgctcattc aatcttgggg gactcagaat ttcatcagaa ttgcggcaat
    12841 ttgctggccg gtttttctcg atctcttcgt tggattcctg agaagatctc tcgcaaaaaa
    12901 atcacctttt gcaggtgatc gtgaccacta ttacgataag atattcagac ttaccaaaaa
    12961 caaaaagaga gtcacgctcc ttatttcaac aggcattgcg ctctcttacg catttgtggg
    13021 tgtctttatt cccgtaatat tcatcccccc tgttcttttg gcagcttcgc tagttcagat
    13081 ttttttcctg ggatcacttc attctactac ttgaaccatc ttattttgaa tagtacaaaa
    13141 ccaaccagga aggcagcaag tcccgacaaa cccatagacc agtagaaaat cgtactgctc
    13201 tccataaagg ggagtttaac attcattcca tagatcgatg ctaccagcgt cggaatctga
    13261 agagagagcg tcactatcgt taatatcttc attactacgt tgagattatt ggagatcaca
    13321 gaagcaaagg catccatcat tcccgtcaga atgtcgctgt aaatgtttgc catctcgatt
    13381 gcctgtctat tctcgataga gatgtcttca aatagctctt catcttcttc atagagcgtc
    13441 agtatgtttg ctctcttgag cttctcaaac attagctcgt ttgatctaag agatgtcgtg
    13501 aaaaaaacta aagatttttc gagattcaac atcgccacaa gttcttgatt cctggtagat
    13561 ctgtgtagtt cggactcgat ctcatttgac agtctcctga tttccttgag atacctcagg
    13621 taataaacag ttgctctgtc aaaaattctt agaagaaagc ggtttcgttt ctttgtggaa
    13681 atatccttaa cccgtccttc gactatgtcc gatacaattg cgttttccga accgcttatc
    13741 gtgacaaaat aaccggattt cattgcaatt ccgagagtcg tcgtcttgta tggaacggtt
    13801 tcgtctgcag tatctaaata tggcagtttg gtaatcacgt agattaaatc ctcatcctgc
    13861 tcgaatcttg ccctttcttc ctgatctaaa gcatcgtaca caaaatcctc gtcgatttct
    13921 aaactcttaa gaaaagcgat gtctccacta tctggcgata caacattaat ccagcagcct
    13981 tctactggtg aatctattgt attcattctt ccattctcgg aaatcaaaat ctcacgcaca
    14041 tcggtccccc cctttttaag tacttcatct ttcttttgtc acttagaagg aggtctcaac
    14101 gggcgaatca aatcaaagtg gcggttcact ctcgctactg tataattcca taccactcac
    14161 ctccgctctg agattatatc acgacgtaat tgaacaccgt tattctgacc gctaaaagct
    14221 attccaacac ttctcttgta tctgtcatcc atcaatggac gagagttcaa aaaacccttc
    14281 aataggaatc aagattcttt tttccgttat aatcattatg tgtccatttg ttcgcagatc
    14341 ccacgacacc ataaaggaga tgagcgcgtt gaaaaaatat atgcttcttc tgtttcttct
    14401 gatgattggt caggtttttt ctgtctattt gaacgatatt tcgcgcgatg gagttaccgt
    14461 aagctggttc accgaagaac cctccacttc ttatttaata gtctacgatg gctccaatga
    14521 atctattcat tcagatgagg aagtgactct tcacagattc cgggtatctg gtctccttcc
    14581 tggaatgagc tatgcttaca ctgtcaagag tgaaaaggat ggagaagtca tttataaagg
    14641 caacgggata ctgacaaccg cacctaacag aaagacacca ttcagttttg ctgcttacgg
    14701 ggatagcaga aacaactatg tcgttcattc tgagatagtc aatggaatag ctagtgaaaa
    14761 cgttcctcta gtaattcaca cgggtgatat cgtctcgacc gatggcgcta ttgaagaatg
    14821 gatagacttc ttcgaagtta ccgaggtact atctgacagt gtactctaca gtgttatagg
    14881 aaaccacgag tctgaagcca taaactattt gaaattcttt gctttcccag gaaatgaaag
    14941 atattatagc ctgtggtacg gtgatatctt cttcatattt ttgaacacaa atgaagcctt
    15001 cgataagtat tcacagcaat acgtttggct tctaacacag ctggaagagg cggaagaaga
    15061 agatcctgca catatagtag tctgcttaca ccaccctccg tacagttacg gctctcatgg
    15121 agaccatctc tatctaaagg aatacctggt tcctgtcttc gaaaactaca acgtcgatct
    15181 tgttcttagc ggccatgatc atggctatca gagaattgag agaaacggtg taacttatgt
    15241 tattacggct ggcggcggtg ctccgcttta cgatatcacc cggggcgaga gtctagtcgc
    15301 ttccgccaaa gccttccact atatgttgtt caactatact ctagaaggac tgtacggcta
    15361 ttcaaaaacc ccggagggcc ttatcctgga cagtttctat attccaaaac ataattaact
    15421 gatctttata acagttcatc gaatttaaaa taattgatct tcgggaaata ttcattttca
    15481 tcctatagaa tcctctctca gtaaggatct tatgtgctca tcattcaccg aatacttcag
    15541 cattttcatg ccgactttca ccagtcgtct gtaatcctct tttgataaga ctctcaaaaa
    15601 gttgaacttt tagcaaactc aaataatccc ccaataacac cctacatgta taaactaatt
    15661 gtggaaataa attcaggagg tgtgaatatg aagaattttg gtttacttat cgttatcctg
    15721 ttactggctg gtactatgtt aagttctaca ctggtgatta agggctccaa cactgtctat
    15781 ccagttgcgc agctctgggc agagggtttc aaggcaatga accctgatgt tgagatatct
    15841 atcgaaggcg ccgggtcctc gacgggtatc aaggccttat tcaacggaca gaccgacatt
    15901 gcaaattcca gtagatggat caaggcctcg gaaattgaac agatgaacag agaaggaaag
    15961 tacttcattc catacgttgt cgcctatgac gggatagcga ttatcgtaaa caagtccctc
    16021 gggattagca acataaccat tcaacagctt tttgatatct actcgggaaa gataacctcg
    16081 tggaatcaga ttgattcttc tctgccaaag gctagaattg ttcctctgtc aagagacaat
    16141 gcatctggaa cattcgagta cttcgtcgaa cacgtaatga agggtgagaa attcgctcct
    16201 caggttcaac agctggcgtc aacaagagcc gaagttgagc aggttatgca gaatcagtat
    16261 gctatcggct acattggaat gggatacatc actgaagagg ttaaggctct gacagttgaa
    16321 ggcgttgaac ccactgtagc aaacgtgaat gcaggctcgt acccgatatc aagaccattg
    16381 ttcatgttcg tcgatgcaac caatggactc cctacaggag ttactggtga attcctgaga
    16441 tatgcactat caccggaagg tcaggcagct gttctaagtg tgggttatgt aaacgcttac
    16501 ggaacaaaat agattggatg aggcgagttg agaagaagga gacttgatct ttcgtcgaga
    16561 gtactggtaa cgactgcagc tttgattaca tcagcaattc ttttcggaat attcttcttt
    16621 ctcatatcag atggggtaag agttttcgga aagatatcat tcgaggagtt ctttttcagc
    16681 gctcgctggt acccaactta cgaggatgcc gaatatggca tcctcgctct gttatcaggc
    16741 actctggctg taacagggtt gaccttagcg atatctatcc ccttgggatt tgtttcggcc
    16801 atatttcttg ccgagttcag ccacaggaag gctcacgact ttctaaaact catatttgaa
    16861 ctcatggcag gaattccatc tgtggtactt ggaagtttcg gcctcaagta tgtctcaagg
    16921 tggttaatgg agttcttccc gatgaaagtg tggacgggac tgaatatttt caacgcatct
    16981 ctgatgctct ctatacttgc tatgccatat ttcgtaactc ttatcgaaga tgcattgaaa
    17041 gctgttcctg tggatcaaaa agaagcctca ttggcactgg gagccaacac aacttcaacc
    17101 gtattccgta ttattgttcc acaagctaga agcgggatcc taaatgcgat catacttgga
    17161 acaaatagaa taataggaga aacaatggtt gttctgatgg tcgcaggcgg cgcttccatg
    17221 attccccatt cgatctttga tccagttaga cctctcaccg cagcaatcgc cggtgaaatg
    17281 ggagaagttg aacttggaag cactcattat tacgctctct tcatgattgg ggttgttcta
    17341 ttagggattt ccctgatctt cacagctata gcaatgcgtc ttaagagggg aatgagaaga
    17401 tgctgatcga ccgaataacc aaattgattt tcggaattat atcgtacaca ctggttgcag
    17461 ttatactagc tataattggc ttccttgttg taaatggact tgatgaaatt agcctggaat
    17521 ttctggtctc cttcccgaaa aattcgatga cggaaggagg aatttggcct tcaatactgg
    17581 gaacatttta tttcttatcg atagcactgc tcttttctgt accggttggt attctatcag
    17641 ccgtctattt gagtgagtac agcaaagaga atcgctttca gcgtctagtt ttaactgcta
    17701 acaacattct tgcaggaatt ccttcggttg tttttggaat gtttggcctg tcgctttttt
    17761 cgatcacttt tggattcgga acctcaatca tctcgggggg tcttacttta ggaataatgt
    17821 cacttcccta catcatttca aatacccatg aaagcctgga tgcagttccg aaatcttaca
    17881 gagaagcatc gatggcactt ggtgcgaata aggctgagac aactttcaga atcgttgttc
    17941 cagcctcctt ctcgaggata ctaactggag tcatgatagc tgtaggaaga ataattgggg
    18001 agacggctcc tttgctgttt acaggggccg cattctatat tactagaaac ccttcaggtc
    18061 tctttgagcc ggcaatgtca ttgccaacac acattttcgt gctttcggca atctatcccg
    18121 agaatgtgac tccgaagctt gagggaagca tttcagttct gctgataatc gtcataactt
    18181 tgtttgtcag cgtcgcaatt agaagaagaa tagaatcgaa gaaactggag gagtgaaatg
    18241 gaagaaccaa ttttcaaaat agaaaaactc aacgtgtatt atggtgataa gcatgcactt
    18301 aaggacgtga cctgtgaaat cccaaaaaag aaggtaacag cggttgtggg cccttccggt
    18361 tgcggaaaat ctacctttct tagagttcta aaccgcatga atgatctgat accttcctac
    18421 agacatacgg gatcagtaaa acttctcggc aacgagattc tcaatatgga cccggtcatt
    18481 ctaagaaaac acgtaggaat ggtctttcag aagccaaacc catttccaaa aaccatccag
    18541 gaaaacgtca cttatgggcc gagaattcac ggaatcaaga accgcaagac acttagggag
    18601 ataaccgaaa agtcactgaa gcaggccgcc ctatgggatg aagtcaaaga tgagctgaag
    18661 aaaaacgcaa caaatttatc gggaggtcaa caacaacgac tttgcatagc gagggctctg
    18721 tccgttgaac cggatgttct gcttctcgat gagcctacga gtgctcttga tccaattgct
    18781 acacagagaa tagaaactct tatagaagag ctctcggata agtatactat tattatagta
    18841 acccataacc ttcagcaggc actgaggatc tcggattatg tgatgttctt ctacgtgggt
    18901 gatctcgttg agtttgacac taccgagaag ataacctcta accctagaga tcaaagaaca
    18961 agcgagtacc tgagaggaat cttcagttaa cgggagttgg ttgagatgac accagaaagg
    19021 cagaggcatc tggaagcagg ctttaacaga ttgaaatcta tgttgtcaga tatgatggag
    19081 aacgtgagat cgagtctttt gaaagcgata gactctcttg atcgattaga tggcgacctc
    19141 gcaagagtag tcattgaaaa tgatgaagtg attgataagc ttcagagaga aatggataca
    19201 gaaatatgca actatatcgg acgatttcaa ccccttgccg aggacttaag gtatgttctt
    19261 acaatgatga agctggcaac cgatctggaa agaatcggcg acctcgccgt aaacattgct
    19321 gaagtcgcag tcaggtatga gaatcagact ctcgtcaagc cacttataca tattaagaag
    19381 atgacagcgg ttgtcgaaaa gatgttggaa gagataatag atgcctacta caacaaggat
    19441 acggagactg caaaaagagt atggaacgag gacaacaacg tagatgaata ctacctgtac
    19501 ataaagagag aagtgagaga gaaactggtt agcatgaccg acccaaatgc gatcggacag
    19561 ctcctagatt tgatgctagt gtcaagattt ctcgaaagag ccggcgatca tgcgactaac
    19621 attgcagaag agatctatta catagaaaag ggcgagagcc tcaaggagga aatgcgacgt
    19681 tgaaagtatt gattgttgat gacgatctcg atatcctgaa tattgtcaga actgcatgtg
    19741 agaatgaatc gatgcagact tcaactgccg caaatgcaga agagttatgg tcatccctgc
    19801 gttcagaaaa gcccgacgtg atattacttg atatcatgct acctgacgca aatggtctcg
    19861 atctcgtgaa gaagattcga acaatgtatt cgagaattcc gataatattt ttgaccgcaa
    19921 gaaagtccga ccttgacatg attcttgggc ttgaacttgg cgcagatgat tacgttacaa
    19981 aacccttcaa tccaagggct cttgtggcaa gaatcaaggc tgtcatgaga agaacagaga
    20041 tatccggtac tttggaagat aaacttacga ttggtgacgc cgtagttaac ctcaaatcgt
    20101 acacagtaac taaaaatgga aagtcagaag agctgacaag aagagaattc gaattgttga
    20161 aactgctcgc ggaaaacccc gggagagttt ttactcgcga agagctactg gacaaggttt
    20221 ggggaatgga tttcttcggc gatttcagaa ccgttgacgt ccacataagc aaactcagag
    20281 aaaaggttgg agagggattc attaagacag ttcggggagt cggttacaaa ttcgtcattg
    20341 gagagcagag atgaacgaat ctctgctcga tgaacttaac gaagcaatca taatggtaga
    20401 tgaaggtctg gtcgtaacaa gggccaacct atcggctaga aagatgcttg gagatgtgga
    20461 aggattagcc ttacctgacg ctctccacgt tcagggcatt tcaaagatag tagataatct
    20521 catttcgggt actaattaca ccatcgacac gatcttcgtt aaggatgaca cgactcacta
    20581 tctcaaaata agggtttctg caccatatat aatagcgaga aacataacga gtgaaaaact
    20641 ctttgaaagc gctaagatgg actttgtcaa ttcgattgtc cacgagtttt ctacaccgtt
    20701 ggctgttata aacggttatg tacagcttct aatagaaaag aaaatggagt tacctgcaga
    20761 tgtttccgag actatcgaaa ggatagcgag atcgactgga cggctttcga gattggttga
    20821 agaacttgga atactgtcta atctcgagct acagaactac agagtcaaga ttgaaaccgt
    20881 caatttgaag gaactaattg atgagtccgt aagtgacctc gaaagcaaat ggaaaagaaa
    20941 gaaattgaag ataaccacaa cagtctcccc taacacatat gccgccgtag attcgcttct
    21001 tctcttcaga gtgatatcga acctgatttc gaatgcggtt aagtactcaa gtgttggcga
    21061 tacaattgaa atcgtctcta gggaagtcga atccagaata tacatctccg tgaaagacaa
    21121 cggaataggt attaaaagtg aagaactacc gagaatcttc gaaagattct acagaggaag
    21181 caatgcaaga acttcgggat cttctggatt ggggctggga ctgtctctcg ttaagcacgc
    21241 tctcaacctt ataaacggat ctatagaggt acgatcgagg tacatgatgg gaacgaccgt
    21301 gaccatcagt tttcccaaga acatgtagag acatcttctg aatttacttc cagataaagc
    21361 tagtaataag actctgacac tttttggtcg ccaccattta actaacagtg catattcgtt
    21421 tatggcaaag tgtgaaatta cttatgcaat aatttttttg atatgacctc ctgaagaaag
    21481 ttcgtcatat attaatggag ttgttgttta atgaaatcag atactcatag tgtttgtctt
    21541 tatggttcgg ctcacgatct aagaaaagca ataaacacct tccttaaaga aggtctttcc
    21601 aaaaattaca aacttcttta ttttggacca gaaagggatg tcattccttc taaagagaca
    21661 actgacgggc tcccctataa cctggatgat cttctaggtt cgaagacatt agagataatc
    21721 gatttcgaaa agtcatatgt gaagaacggc gttttcgatc ccgtaagaca gattagtttt
    21781 ctgcaagaag ccgcagtaag ggctataaag gaaggctata atggcctcgg tgtggccggg
    21841 gaaggtactg cgatcctgga atctgggacg aaaatggaag agcttctaaa gtacgaggca
    21901 tctgtgagcg atctcgttga agagtctcca gtaacggcat attgccttta tgactcttca
    21961 agaattgacc aaagcagtgc tctggaaatc tccagactcc atccgtacac tctcagagaa
    22021 agtgggttta gcgtaaatga agattattat cgtgcaccca gaagaactct ggaatatgat
    22081 accgcaagtc ggctcgacat ttgcaggggt accgcaaact cccttttaca gatgttcaga
    22141 atgaagagca tatatgtaga agaacatgag gaaaacgttt cgaagcttgc gtcagcaatc
    22201 gctaaggaaa tggcattgga ctcttttacc atggcctgtc ttgaaatggc cggaaagata
    22261 cacgacttag gaatgaactc gatgtttttg gacatgctca ctagacccgg gccactgcat
    22321 gctcttgagg ccagacttct tagaagacat cccattacgg gattcgagat aagtagagga
    22381 gttccatttc ccgataaagt ctcgtacgca gtctttcagc atcatgagag aattgacggt
    22441 tccggttatc cgaacggcct aaaggattcg gaaattagcc ttgagggtag catacttgcc
    22501 gtttctgaag tagtcgcggc aatgagtttt tacaggccat acagaaagcc gtatggacag
    22561 gagtactcgc tggatcagat agtaaaatac aaaggttcac tttacagaac agaagttgtc
    22621 gaagcctgcc tcactgcttt cgaaaatggt ttttcctttg aaagcagctt gaaaacttct
    22681 gcttcccagc cctagtacca aagcagctat tcggtaattc tcttgtcaaa gtggacgttt
    22741 ttgctacaga cattgagaaa acctttggct gtgttcaacg gttgaactag aaacctaccg
    22801 gagagagtgt gtttagacct tcagactaaa gatatctttt cttatcaagt gttctatcga
    22861 ccttttcaag agtctgcagc gttccgtttc ttagttttat ggacttctcc agaagaagtt
    22921 gtattaccgt aagctggcag actctgggtt gaagaagtgg tatcccggtt tcatgacccc
    22981 cttgagctac gtagatggtc tcggtggaaa cactcactat tctcgatcct atcccacccg
    23041 taactgctac agtgtacgcc ccggagtctg aagccacttc aatggatttc acagtgtccc
    23101 taatattccc gctggcggag ataccgaaaa ctatatctcc cctgttaagc gagacggcag
    23161 tcattatctg caaatggggg tctgtgtaga acgcggtcga aaaaccggct ctcatgaaga
    23221 gggttgcccc aaattcggca actattccgc ttgaggccac tccaaagaaa atcagtctat
    23281 tcgctctaac gattttacct gcaattctgt ctaattgaac atcatcaata agttggttct
    23341 gctgattaag tgcctcaatg agatcggaaa tcagcttatt cttgtcggct gaaattggcg
    23401 gtgattctcg cgagagttct ctggctagct gtattttgag ggcctggtat ccattgaagt
    23461 tcagcttttt tgacagcctg aaaattgtgg tctcgcttgt tcccgcaagt actgagagtt
    23521 cgcttattga atgatggata acatcatctg gaaaatcgat aatgtatctg gctgccttct
    23581 tctctgcagg gtttaaaccc tttatcatcg ccttcagttt ttcagttatt ctgaactctt
    23641 ctcccatata gatatcaact catccttcgt ttctacaaac ttcttcagaa gtttatcgat
    23701 ttcttcttcg gcaccttcct gggtcatctt ttctgttctc gagacaaagc tggcaaccct
    23761 tccaaaataa agcggaacta gaagctctat gagtctgtct ctaagagcta actttctata
    23821 gcctgaagct attctgtaaa taattctaac ccaaagatca tcatcgatca tcgcgtctga
    23881 ttttccaaga agtaagagat ttcgagcaat cttcgcacca agaatgtctt tgatgaaatc
    23941 ttcctgactg tgtatgcctt cgtagcactt ggaaatcatg ttttcgagat cgacctccag
    24001 cggctccggt atacctgtca caacgtcacc atagataggt gaatcggaat agtcacccac
    24061 tgttttccaa ttatcctcat atttctgcat cagggaaaac agggtgccta caacttcttt
    24121 gaacatgttc cccaattgct ttccaggatc tttgacatca tgaatcttcg tcccgagagc
    24181 ggcttgataa atgcttcctt tgacttcatt gacagcggag gtcgtcatcc atatatctat
    24241 tccaaatctt gaaatgtccg atctccacgt attctcgggt accgtcgtgt agtattcgag
    24301 gagctccctt ccaactccga aatctccccc gatgggttgc ctgatctttt ttccataaag
    24361 agctgtggtg aggggataac atatgctgtt cgttattgtc ccatcgtact tgtgtcttat
    24421 atagtaaggg gtgacatagg ctgccccttc ctccagaatc ggacttgcaa gcctttctat
    24481 ccagaatggc tccacacttc tgagatcgga atcaagaaat aggactgctt gcgctccata
    24541 tgattttgat ttctccatta ttgatctcat tgcgctacct tttcccgaaa ttccctcata
    24601 tacaaaggac agcaaatcta ccccattact tgaagccgaa taaaacatct cctttgtcct
    24661 gtcggttgag cccccgtcag cattgacaat gcaacctctc atagatggaa aatactgctt
    24721 taagcctatg gccgccgtct cgacaacata tttaatcgta gtctcgttgt tgaaactagg
    24781 tattcccacc actatatcgt atgaatcatg actcagtccg ggcatataga cacctcctat
    24841 tctttcacgc ctccagcagt gaatccgctg gtcaggtatt tctgcatgag actgaagatg
    24901 ataattattg gaactgcgga aaccacagaa gccgccatca acctacccca aacagcatgg
    24961 gcggaacctg agaacgcgtt caacccaatt ggaagggtga agagatcttt gtatgacttc
    25021 aaaccaagga aggccgatgc gaataggtat tcattccaac caatcataaa tgcgtagata
    25081 tatacagtca ctatcgctgg aagagaaaga ggaattacga tcttgaagat cgcctgaatt
    25141 cttgagcaac catcaatgat agcggcttct tctatcgaaa acggtatcgt tctgaaatag
    25201 ttgcccagca tgtacaaaga aaccggcaat gtttgaataa cataaaccat gatcaagtag
    25261 attcccgttc ccaatggcgt tgcaaggatc ccgagcttca ccgccatttg ataaagcgga
    25321 accattatta ccgtccctcc aaagagataa acgaatagaa cggatcgctg aacagaactt
    25381 ctccccttga atctaagcct cgaaaaggcg taggcaccga aaatggcaac aatgaggctt
    25441 attaaagctg aaattcctgc gaagactaat gagttactaa gatatctcag gaaggggaat
    25501 tcacttgctc tctcctcgat tatctttttt ttctgagctt cataggattt gagctgggcc
    25561 tttgcattct ctagaccagc caggagagac tcctcggtta gagcgctgtc cacttcttcc
    25621 ctaagatcga taaacttctt tacatcgttg gcactgaaaa tcgatttcat attctcctca
    25681 acaatttccg tctcttcggt acctggagtt atcgcgcgtt cgataaattc ctctctgttt
    25741 tgaacttcta ttccactgaa atcaattaca agatctacaa tatccctaag ttctttctct
    25801 tttgcctcca actggatctc gtgaacatat cgagtgtacg attccagtga ggccgtcctt
    25861 tcttccagct cttcgattct tttcagtacc ggctcaagat ttgccctaat aatttcatct
    25921 ccgaaaaact ctgtctcgct actctgaatc aaaccgaata tctcgaggta atttcttacc
    25981 gtgaagttcg aaaatgtcag cttgtttgga tcataatcgg cttttaatga tacatgaatc
    26041 atgaaataaa acggaattag aataaaaaag cacaaaagaa ggatcccaaa aataaacatt
    26101 cctctcttga agggtgattt tctctttacc atttcaacac cttcttgacg tacacggtta
    26161 tcaagaaaag cattattacc atcagaatcg ttgctatcgc agcggctact ccgtactccg
    26221 gcgaagtagt gcctattacc ctttcataga tgaagattgg tagtgttccc gccctcttgc
    26281 ttatcagata gacttcatcg aatttgtaga aattccagat ccctctcatt agtgctaccg
    26341 ctcccatcac aaagtagagc tctggaagtg ttatattagt gaactgcttc caagacgttg
    26401 ccccatcaat ttcagcagct tcatagtaat cgtcaggtat cgactgaagc cttgatagga
    26461 gcatcaaata gacaaaagga aagtttcgcc aaatattgaa gacagaggcc accaagaacg
    26521 cgttgttcga attgttcaga agatcgagtt gaggggaaac ccacccaaga gtttgactga
    26581 acagctgcat aaatggccca tagctaggat cgaagatata cttccaggag aagacaattg
    26641 aaatgagcgg tgttatgtat ggaagcagca caatcgtcct aacaatagct ctccccttga
    26701 actccttgtt gagcattaat gctacaacga gtcccaggag aatgcttcca atcacagtca
    26761 taagcgtgaa acctacagta atgaagaatg acttccagaa ggcgctgtct ttgaagacgc
    26821 gaacataatt ttccagtcct acaaatgtgg agctggttat tccataatcg aagaacgaga
    26881 tataaatatt gtaaccgact ggatagagta taagtatcat taacagtatt acagttggtg
    26941 aaaccagttt ccaacccaga ctgttttctt tcttcaacat ggaagaggct gctctctgca
    27001 tgagatctcc ctccaaaaaa tgaaggcccc ggctccgggg cctccaatgc tagatgatta
    27061 tcttcctata agcttcctaa tatcctctct ccagaaagtg agaacttgat ctggagttgc
    27121 gtcgttatca aacatcatga caattccatt tcctattgtg aaggccccgg atatcttgcc
    27181 catttcaggg aagaccctcc cttcaacgta accgaacttc tcgatgttct ccattccata
    27241 gatgattgac ttgatctttt cagcaccgta cctcctgtaa atcccttttg gatcattcag
    27301 gaattcttca ctttcggcga tagaggagcg cgttggaagc ataccaccag gagccatatg
    27361 gatgtacttg atgtagttgt ccggttcgaa aacaaaatcc agaaacttga gaacgtcaag
    27421 tttattctcg gaagacttta ttactgcaag cgacaaaacc tgtccaaagg atgaagatct
    27481 gctatgagtc atcattggag caaactccgt gttctcaacc agttcaggat caaaattgct
    27541 gatgtattga gtctgaatct ccgcaagggc aaggtcatcc ataatgtaag tagagtagaa
    27601 catcatcggt gttttacctg cgaggaaaag ctctcttgct tgctgccagt aggtgtgccc
    27661 gggagccgag tactctccaa ggtccttgta aaactggagt acttccttca tctccggaga
    27721 attgactatt acgtttcctt ccttatcaaa tatcctggct ccatttgaca aagcgaaaat
    27781 cgtgaacacc tgttctgcat aagcatcgtt tgcctttccc agaacgatcc cgtacattcc
    27841 gttctgagga tcatggaaat atttcgcagc ttccatgatc gcttcccatg tggtcggctc
    27901 ggccaatccg gctttttcaa accaatcctt tctgtaccag ataccctgaa cccatccatg
    27961 gtaagggacc gcaaagtact cgtccccggc tggcgtaata acgaatctcg tggcgccgtt
    28021 gtagaaatcc cctccattct taatgaatct cgttggaatt gcagtgtcaa gcaaaccatc
    28081 cgcgccaaga cccagaatgg tttccgcgcc agcttcaatc agatccggaa gcgtttcggc
    28141 agcggaagcg gccgccagcc gtgaaaacat ctcattctct tcgatcgcga ctacgtcgac
    28201 ttcgattccc gtttcaatcg tgaacgactc gactatctgt gcaattacct gctgtcttga
    28261 ttcctcctct tgagtatgcc agtagacaag ttttgcagtc attgtcactg aaagcatcaa
    28321 tacgaggaat accacaaaga aaattttttt cactgtcatc acctccgaag gaaatttttt
    28381 tcatctctat aataccaaac tttacatctt tgaaaaagaa caatcaagat caaccacgtg
    28441 cgatcaggct cattctagat tgtctatagt taattacgcg cagaaaacat cgaataccta
    28501 gagacaatta ttctgcccgt ctttcaaaag atttgtggtt taagctcggg attctatgtt
    28561 tgaggcggca agcaaaaaaa gaagaggtcg gttgtcccga cctctatccg ttaaatttat
    28621 ttcatgatcg ctgtgtcaaa ggctcttgtg gcagaaccac cagtcgtagc aaccttcctt
    28681 gccctttctc tctgctgcag tcttgacgtc tgctgcaggg ggaataatta cgtgatcgcc
    28741 gatgatttcg ttgtttggcc aatcggcagg aatcgcgact ccgttcttgt cggcaacctg
    28801 gaagcccttg accattctaa gtatttcgtc catgtttctt ccaagctcct gcggataata
    28861 gagtattgcc cttatttttc ccttcggatc aacaatgaat acggccctga ccgtgtttgt
    28921 acccttcgca ggatggatca aaccgagagt cataccaact tcacccattt catcggcgat
    28981 tattgggaac tcaatcttca ctcccagagt ttcttctatc cattcggccc actttatgtg
    29041 agagaagact tgatcgacgc tgagacctat gagttcacag ttgagctctc tgaacttctg
    29101 gtatctcttc tgaaatccca caaactcagt tgtacaaacc ggtgtaaaat cgcccggatg
    29161 gctgaacaaa acaaaccatt tgcccttata ttcttctgga aggttgagta caccgtgagt
    29221 tgtcttcact ttcatttcag gaaaatcttc accgatcaaa ggaattcttt tctccataaa
    29281 acacctcctt atttagaacc attctaagta tcaacatttt gattatagtg gagtgttttt
    29341 atcctgtcaa ccgtacaaaa aaagagggcc cgcggccctc tttttatggc ttcaacagag
    29401 tagatagggt gtttcttctc catctagata gatgtatgaa ctaactactc tgtaatctgg
    29461 aaaactcgtt tccagcgctc ttgagatcaa tgataggtac tcgttgatcc ttggcactac
    29521 ttcttcgaaa gagcacgaac ctttgagagc cataatctga acttcaatcg tgtcttccgt
    29581 ctcggctctc atattaagcg tgtcaccttg ggctcccaga agcaactttt ttgagagcgt
    29641 ctcaagtatc attttcaaag cctccgtgga tctactttca gaaacagcat acgtcaaatc
    29701 acacacaata tacatggcgc tccctccttc gtttggtgtc tttgattttg ttcataacta
    29761 acataggaag gtaccagatg tagatcctga tttctggaaa agccgttttt gaaagaccat
    29821 ttgtaactgc aactgcaaag tgatcagccc gtttaaacaa ttatacctca aggactgtca
    29881 accttaatta gatccgttat agcatattct cgagaatcac tggttgacat acagagaatg
    29941 aagtttaaat ctaactcaaa gggagatgtt cttaatctcc ttcatcttga cttcgagtag
    30001 attgttttga tgcattaata acattctcat gagaagattt aattatgatg aattacgtgc
    30061 tcatggaagt ttgcaatgtt ttacaagcta ctttctttcg aatcattaat gtaaaatgta
    30121 aagaacaggg gtgaataaaa tgatttacag aactttggga aagaatatgg gagaaattcc
    30181 agccataggg attggtacat gggaaatagg tggaagagat cttcccgatc tatctagaga
    30241 caaagagcat atcgacacaa tatccagagc aattgagatg ggttatacgc acatagacac
    30301 ggcggaatac tatggtggag gccataccga agaaataata ggagaggcta tttcggtctt
    30361 cagcagagaa gagctgttca ttacttctaa ggtttggccc acccatctgt ggaaggaaga
    30421 acttcacaga gctctagaag gaactctgag aagactcagg acggactatc ttgatctcta
    30481 ccttatacat tggccaaatc ccgatgttcc tctcgaagag accctgaacg ctatgagtga
    30541 agaagtagtg aatggaagag tcaagtttat tggcgtttct aactttgaca cacctttatt
    30601 gaagaaggca gttgccgttt cgaaacaacc aatagtaaac aatcaagttc tcttcaacat
    30661 tgacgataga cagccaatgg aagagcttct tccttactgt cagagtcatg aaataactct
    30721 gactgcctat tcgccgctta aaagaaactc tttgaaaccc tcgaccgaga agctgctaaa
    30781 ggatttcgcc gtgaagtact ccgcaactgt acagcaaata atgcttgctt ggttgttggg
    30841 caaagaagga gttgtggtaa ttccgaaggc atcgaagcta gatcacctgc gcctaaatct
    30901 cgaagcgggg agtatagtcc tggatgacga tgatacgagg agactagatt ctctggaata
    30961 aggaggttac cttgaacgaa atcagaagta gtttcataga ggctctagat tctcaggaca
    31021 aaaagagagc cgtagaaatc tgcatcgacg ccctggattc tggtagcatt gaagtggtag
    31081 atctatatct ggaaattctc ggccctgctc tcggaaactg ggagtgcgac tatgaaagtg
    31141 acaggatgtg catctggaac gaacatataa gaagctcaat cgtcaggacc gtaatggaat
    31201 gttgctatcc ctacatcata gggaaaagag gcggcaaaag atctgccgag aaagaggtta
    31261 ccgttgcagt tctctgtcca ccggaagagt atcatgaaat aggtgccagg atgatctcag
    31321 acatcttcac gattgcagga tataactcga cgtttgtagg tgcaaatacc cctctcgaca
    31381 ccttcctcga agcgatgaaa gagcttaatc catcctatat tgctattagt gtttctaatt
    31441 actacaacct cataaataca cgtaggatga ttaccaaaat aagggaacac ggtggaaaat
    31501 caagaatagt ggttggcgga aaggccttcg agaagaaccc caattactgg aaggaagtgg
    31561 gagccgatat tttcctgaaa gatccgagag aaatcttctc attcggggag ggtgataaga
    31621 aatgaaactg gcattttcaa tcgcaatgcg tttcctatca tccagcaagg tacagactct
    31681 tctcatagtt ctagggattg caataggggt atccgtccag gtatttatcg gctcgctcat
    31741 tcagggactg caaaaggacc tggtcgacac cacaatcgga agttcttcac aagttaccat
    31801 tacttcatcg gaagaagaca atagagtagc agactgggag agtataattt cggagatcga
    31861 ttctctagat cttccaacgg aactaacggc tctttcggcg agcgcggatc ttccagtatt
    31921 tatcgacagc ggcgacagaa ccttatcggt tcttcttcgg ggtcttcagt ttcccgaatc
    31981 aaatgcaata tataagatcg atacacgtct agtagatggt aaaatcccgg agaattatag
    32041 agagatctta atcggcaagg gtctaaaaga ggatctggaa gtgaaaatcg gtgaaaaaat
    32101 aacgatattc actccagaca gaacagtcga aaccctcgaa gtcgtcggca tttttgatct
    32161 gggagttgca agtctcaa
//
LOCUS       DAOQYI010000020        29063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1886_length_29063_cov_78.015203, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000020 DAOQYI010000000
VERSION     DAOQYI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 29063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29063
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1886_length_29063_cov_78.015203"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="PKL93_06745"
     CDS             <1..1803
                     /locus_tag="PKL93_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNQ70992.1"
                     /translation="ERYEYRKDRGDWISVGTDLSYTWRGYSEGNHVFEVRALDDGGAY
                     SQIVIWSFTYSYANQPPVITKTGGLEGDIYVSSNSFSWTGSDSDGTIAKYEYRKDNGD
                     WVDFGLGTNYTWSGYSEGSHSFRVRGRDDRGAYSEEALWSFTYSIPPQEMGAFKVVNS
                     WGVGGWENVPDGFLYITYEAMKENQVRCFTIDPREDYEPRAIAVFEISHGIRDDCEIT
                     VGIGNPSSPIREKRFDDYSYRGGQHPFPDNKMVLDITELLPFEDETLFLKVFDSFSDF
                     TTGSIEFFSVEVFDNYQSGTPVAIYTSTETPKNTVNNSFVNVQIHNVVAAQGSSYYLS
                     SIRQGLSTEMLELLKADLGVLEEGRNYNEIIDGHGTGLRPPSEDDWDEIARTWHLMDG
                     FSVQGSLPSTLDHSVSMYFPPVGDQGGEGSCVAFSNGYYTSTFYEARDRGWDLSGASW
                     TNGGEPTPSYQNRIFSPDFIYHQINDGEDGGSSYLDAQKLLSRVGVSSWEKMPYDTSD
                     HTSWPSESAWREAPRYRNGMNVISYLTVRTDQDILTIKSYLAAGYLVSVSIDANQYKN
                     LTEKDVWNTSTYIYPDTNHANTIVGYDDNFNGSL"
     gene            2181..7088
                     /locus_tag="PKL93_06750"
     CDS             2181..7088
                     /locus_tag="PKL93_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNQ70993.1"
                     /translation="MKRVLVHILGSVRSWLLIMLLIPFGLLFSENIETPVDNYKPPTE
                     VSGTIAEDTVWTIDRSPYLVRATITISPNVILTIEPGVEVLIVQNQDFIVNGTLRAVG
                     NEENPIVFTGTAQVPGWWRSINIQGEGSAFLEWCEVSFAGASTGAGVLKAGSGSLRLV
                     NSVVRRVMGDGLRISAGYSSFESINNTFMYNTNGVRVGINASYSDQSSTFILNQVDIH
                     LDGGAITGIVKWGASGDYSMTVTGDVSVNAGASLEIAPGTVVKLRQNNRFLVYGQLQA
                     RGEEDQQIYFTDLRDDTVGGDANRDSSETIAEEGWWRSINIQNEGSAVLEWCTLAYGG
                     RSDGATLLKSGTGSIRISNSAIVRSSGDGLRVAAGYSLFESSDNYFGYNSVGIRLGIN
                     SSFSDFTSRFESNDLDVHLDGGTISSSVVWGASSEYSMVTGGDVVVAAGASLKVMPGT
                     VIKLRQNNRILVYGHLEAAGQESAPIYFTDFRNDLVGGDANRDGEATVPASGWWRSIS
                     LLTEGTANFEHCTIGYLGVSDMAGVIKSSTGTFSILNSTIHDVTGDGLRIDNSAGSAE
                     VKYSTLSYNTGAGLYYKTDGVHIEALTISSNGIGIRLPAGCSVEVDEQTYFDGNDIAI
                     QIDPAAISGRVTWGAPSNISILMNGSVSVASGASLTIRPGSVIKIAQNAFLAVDGELM
                     ASGTEDNPIFFTETRDNSVGGALSGDGSLPEAGWWRSINIRNEGKAIFDWCTIAYAGR
                     SDGAAILKSGAGSLRISNSVLSHTSGDGLRLSSGYSSFEHFQNEYSYNTIGVRLGINA
                     SFADVTTRFEGNSTDIQLDGGAITGTVSWGSDGDYSMVITGDVSISAGASLSILPGTV
                     IKFRQNNRILVYGSLQALGEADLPIYFTDLRDDLVGGDANRDGGETTPAPGWWRSIGI
                     MTDGTATFEHCVIRFAGYGDRSGVLKSSTGAVSMTDTVIEKVAGDGFRADNAAGGVKI
                     GRSTFSENSEAGLNCRTDGVSVEESVFLSNDTGVRTVANASLILDDETYFSENNKDIY
                     VEAATISGSVVWRVPKHLSLLLSGSTSISVGARLEIKPGTVVKMAQNSLITVDGELIA
                     LGTGEEPVFFTDYRDDSAGGDSNRDADATLPVKGWWNSINIRESGSAELDFVDIGYSQ
                     NGIVRAGSGSFTLTNSNIHDVSGEALKLLSGYSYLELSNNVFLRNGAAVRLATNVSFD
                     DTLAAFEENGTDVYVDGGEINSNVVFGLSSDYSFYISGELTLTKGAVLEIRPGTVLKF
                     AANRGLRISGSLKMLGTEQAPITLTDWRDDSTGGDANHDGDASLPEPGWWRSIYIQEE
                     GSAELEWARIMYAGYGDKAGVFKTGSGSLILRNSVISRIAGDGLLVSSSSGHLEVSGL
                     SFLENSTGVGISSQQTVPLEFVDCVFEGNVVYGIRNDSPVEAVAVDCWWGDISGPHHP
                     SLNNRGTGNPVSDNVSFDPWIGKEELMNESESEEVPSENAQEVVQETVVAEWIEVSSD
                     GLCYEVPSSWFDDSEGYRLELEGDENSKPVSRWFDAEPGSENIFFVIAKVKPEFLEKE
                     EEETRLNMEVVEEFERLIAGEPGIWIELTAAQWNVERSIYSHVYEPGNDGYCLSVGFA
                     AKTGFWTQYEPVLERIMETLRYCGN"
     gene            complement(7397..8740)
                     /locus_tag="PKL93_06755"
     CDS             complement(7397..8740)
                     /locus_tag="PKL93_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNQ70994.1"
                     /translation="MKKSLKFWDLVALEVGMTIGAGIFVYMPIAYKSAGIGTIVAFIF
                     AFLPMAIIMINVMLLGSTLPTTGGTFKYGAFLFSPKVAFLGLWAYLFGAFVGLFPLNA
                     LALASYMKGIWNGISVVPVALIILTFFYVVNLLGLKTASIVEIVFVALLFVAIAIYTV
                     PGVAKIDPRNLEGLFSTGLGPMIYASALLTFTFAGSNAVIELGGEVENPRKNLPLSVM
                     LSLSIVLICYLLMAIVSFGVGEEVMANGTLNDVASNYLSGFLFYVFAFGGPILAIATT
                     INATYMWGTRSLLALCKLRVFPSKLGLVNKRGTPWVLLTIIWLLSSIMLISVGESGLN
                     LFAAFASIGGIAVIIPTMFAVFKLKKNETLRKTAPAIVKKKWFIFIPILGVVFSILIM
                     LILLYQVGVDFSTSFFLFFIVWEVIGMIYFIFRLKYLDRTEKNVFSRNDLSVFDD"
     gene            9349..9972
                     /locus_tag="PKL93_06760"
     CDS             9349..9972
                     /locus_tag="PKL93_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HNQ70995.1"
                     /translation="MGLYAVITADIIQSRKQVLPFERIEQTLASFAGEHLVKSFALSR
                     GDEIQGVVSDLSVIVSLVRRLRYVMWPLALRVGMSIGEIEDDKLKKAGTSWDLSGEVF
                     FSARDALDMAKKSRVSNTFFLCSDELLSTALNTSLSLLETIESGWTEKQWQAVHVYER
                     EGTYEKAARVLGVTAPAVQQHCDKAGWNVVRVAEKELARLISLSLKI"
     gene            10101..10889
                     /locus_tag="PKL93_06765"
     CDS             10101..10889
                     /locus_tag="PKL93_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNQ70996.1"
                     /translation="MNGFMIPLALSILAHVLADFLFQTDNTAKEKSEQQAKGFLKHWI
                     VVFLTLLVLMMPFGLMDVLLYCVVLSLFHILLDVFKSSIESKRGPATRFSMFIIDQLL
                     HLMLIVFLVPISSFTIARSFSAFIVWIDFHTGLDLAVLPVSKMLSVLIVYLYVLFAGA
                     VFIRKLFDLIYKNQPDYLERIVGNSSSLDNVRTGKAIGIFERLLVLTLYLTGNVASIT
                     IVIAAKSLARFKNFDNKDFAEYYLIGTLASVMIAIIGGMILEVL"
     gene            11819..14713
                     /locus_tag="PKL93_06770"
     CDS             11819..14713
                     /locus_tag="PKL93_06770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ70997.1"
                     /translation="MKNRLISLFLAAIATVFAAVIIFAMTEVRYSKIENHIANSARII
                     TENNAKTAFLGYFHWDSMYEALESGDADFVIYNLEDLLKNNSLIEGVVVVYRNEEFVS
                     AGESFQTLRDHTIGASAFYEIIPSGEDLLATMRVTDSAGLESLKDSYAILKLNLDEIL
                     RLLTTEDYRLSSVEGTSVFEGISLSIIRKWSPANYATVSLVFLMSLSFTMIVQRLEGK
                     RSIASKNNEMLLLLNHIPTLIWYFKDSETFGIVNKSFAEFFGMRPEDIEGRKIREIFS
                     GEDLSTSIETNKLVFAGKEEHIYEQDTRNSKGELRTLIVTKTPEIDENGNIKSVVCSA
                     CDVTDERKALRRIELIQFGLDNANDEAFWIAPDGSILYANSAACKSLGYSREEITAMK
                     VNDLDKSLEADDRKSSWERLKSNGKDNFEAYHLRKDGSTFPVEINRNYFKYDGREYEF
                     TFARDISDRLKSFEILERDKFRIERLHEAALNLERCSELQSVYDLVIEAAKEILEFDI
                     CFICVREEENLVIKASSNLNPRDPLEMSIDKGIVGRTYREKKSYVIDDIQEFPGALRT
                     NDIYRAGLSVPVGDVGVFQAMSVKKAIFGEQELRLAELLMSHAREAIVRIETEKRMNY
                     MSLHDKLTDLYNRLYFEEELKRLEGSRFYPISIVSADIDGLKLINDTMGHSRGDQILI
                     ELSQILRSCFRKTDVVARFGGDEFAIILIKTDEEITEKIALRVRKIVEKFNMNNSGPP
                     LSVSMGIATSNGPEQELVETLKQADDLMYRDKLYRSSSVRSQMVNTLLVTLAEKDQIS
                     GGHAKRLQKLCLELGRRARLSTRQLSDLALFAQVHDLGKVGIPDSILFKPGPLTADEW
                     RIMKLHPEKGYRIAISSPDLSSVADLILKHHERWDGRGYPLGISGEDIPIECRILSIV
                     DAFDAMTNDRPYSRAISKEEAVEEIVNCSGTQFDPKLVQMFKSIVSNELV"
     gene            complement(14710..16221)
                     /locus_tag="PKL93_06775"
     CDS             complement(14710..16221)
                     /locus_tag="PKL93_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ70998.1"
                     /translation="MAPLNDLKLFFKHKLFLFPGKRGNRGGLSGQLFIFAVFAIMIGT
                     FLWEVYGSTAGVSIEGVSLGKVMAGFFLTISGFFFLISFSATSSYLFMRNEEIDMLLV
                     LPVKRVSIIAYQIMISTVYQGLTLSVFIGIALPFHLRVDPSPWIGIPALVLVVIDTVL
                     LASIVSVAFGKFMSRAAARKVMFVVQIIAGFMFFIIVQLVPRSTDNIPLYLQKLASAW
                     GVLSNPMNVFTWSVKASEQPIYLLVGLGAIPVLGYIFFRVASSIKFEPVSYTKSRTKT
                     VSFNGRSRGVLLRRELKIYKRYEQLIYYLFYPVVFGLIFGLISDDAVASIFTTVLIST
                     IFTTIQSAFLMGREFPFIETTKTLPISLGRMIALKATLPVLLGSVLFVGVLTTSILVK
                     GESFGYFVVVPIVFVLYITSSLLGIRGVLVSPPDQMDNPNTFMRTKFVLLNQVICMGL
                     SFGAIMPLTTILSGASESNSNIWLILISLASLAVAIVISILSYKKVKLVINEI"
     gene            complement(16223..16978)
                     /locus_tag="PKL93_06780"
     CDS             complement(16223..16978)
                     /locus_tag="PKL93_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ70999.1"
                     /translation="MISCNKLTKVFDKDPVVNEIDLSINSGEIYGFLGPNGAGKTTTI
                     RMLTGTLRPTSGEVRILDMDFFRNELLIKSRIGVVPDEPRIYSYFTGAEFLEFIMDVF
                     PDKKQEAKKRIGELCEAFGIDYLGKMISEMSHGMKQKIMVISVLMRRPEVIFLDEPTV
                     GLDARSAKILKMLLEKYKNEGSTIFMTTHVLEIAEKMCDRIGIINKGKLISEGTMNEL
                     RRKAGADERETLENLFLQLTGEDEDIQEIVSAL"
     gene            17162..19282
                     /locus_tag="PKL93_06785"
     CDS             17162..19282
                     /locus_tag="PKL93_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNQ71000.1"
                     /translation="MRRALSFAILAVVVVVLLFGINGFSFDPSPAAFLSDDDSELGAF
                     NKIAEVFGDSGSIVLVLEASQTSLELLKSITEKINSLEWVKSVLSPTEAVKLGSFNLF
                     TMSIPSESYVYEDEGRLVLNEELFTDPVYSNLIVSSDGRYYAIMITIAEGNELKSDMM
                     IPELRSVLDSSGVESYKLIGESVANFETFRSIFDLTFKYPPFILLAIMAVYMIKSRRI
                     SLSLLTLVPPLAAVMVIIGIMGLLKLSINSLTVMIPSFIIIIGSAYGMHFLSRFEENI
                     HMKNAVRRTIHEERVPILFSALTTMAGFSSYILLDMKAFQEMGIFVCSGIFLSAIFTI
                     TVLPGLVTPKVGTKREVLPPRDVHPLVKRAVIWVVVATSIVSPLLIITIPMTIDQYNF
                     FKEDSEIRESARTMKEAFGWLTNYTLMVEPAEGGSISFTGDQVENLEAFERELKGIEG
                     VSKVLSLFDLSRETNVPLPLLIRVLNSTEAFGDSTSLLVSDNAIRFNIFSSQSDSLSA
                     ERLKAAVEKAIRKFPEMNKNFEFTLAGTTLIWESVNSSVVKNQIQSLIVSFGLIILLL
                     FSIFRSLKSTIIATIPILLTALFNFVFMAVFRIPLSISTALISGMLMGLVIDYAIHFT
                     IWFRRFGDSHKAYEQTARAILTNGISLVAGFSVLLLTPLLLYVDVAKLMVSGLAVGMV
                     LTLILLPEIASRSERFLSRAKDSD"
     gene            19353..19874
                     /locus_tag="PKL93_06790"
     CDS             19353..19874
                     /locus_tag="PKL93_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNQ71001.1"
                     /translation="MDRFVREIWDELKTEFGFYSVFLTPFPHITYCIVEEEETGIDPL
                     IEVIASSTPPLMARTEYLGVFSGEKPVIFIGLARNSRLASLHHALWIKLRDSVSNRAI
                     AYDEDHWIPHITVVFGDDLKRSNIGPVMERLVFRDFHHEFTIDNLTILEEVPDEGIVV
                     RKKLKLVGQSKKD"
     gene            19979..22348
                     /locus_tag="PKL93_06795"
     CDS             19979..22348
                     /locus_tag="PKL93_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ71002.1"
                     /translation="MRKVAWKDIDLKIALPEDVKSTECIGELEEFIGQERAIRALETG
                     LHIDAKGYNVFVSGTTNTGRRTFVSRYLKKKVEGTKTPGDWIYVYNFDDPRSPNSISL
                     EAGTGKIFQKEMNDFVEIAINSIGESFQSEDYQQKVTSLQNEQSEKRSNMLKELIEKA
                     KEKDYTVQINQTGVATIPIWNGKPLTQEVYEALPEDYQKQITKKGEEVRELVNSYLLK
                     LSKMEKDFGEKYKELNRKVASFAVEGHIREMKDRFSESKEVVDFIESMKKDLLDNLGV
                     FFSQEIDSKAFFGKRYAVNLFVDNSGIEGKPVVEVTNANYSSLFGRIEYVAKMGMLDT
                     DHTMIRPGAIHSSNGGYLVLDAKNVLSEPYVWQTLKQVLFSGLEGIENLEHKIGLLST
                     VSLKPEPIPLDIKIVMIGEPWIYSMLSTLDTDFKKLFKVKAEFDWEMTIQNDTVDKLC
                     GLMCNIVKANSLRPLEREGIIEVIKRAIFLSGSRKKVSTQFGALEQILLESSAVAEVS
                     GHEMIQADDVSTAWEEMHKRVSLYQDKIKEMFQNSTLMVQTEGELVGEINGLTVIQTE
                     DLSFGIPVKITAKVGPGNSGIVDIQKESELSGHIHNKASLTIQGYMSSKYAQQFPLSL
                     NGSISFEQVYSAVEGDSASVAETIAFLSAIAEVPIKQSIAVTGSINQSGRVQPVGGVQ
                     YKIQGFYDLCKIKGFNGQQGVIVPEANSDNVILNDEIIGAIKEGRFNVWTIETVDEAI
                     EILTGMKPGKIGKNGQYSSGSFNRLVVDRLKKFYEIASRTHDRSGKDAD"
     gene            complement(22431..23441)
                     /locus_tag="PKL93_06800"
     CDS             complement(22431..23441)
                     /locus_tag="PKL93_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNQ71003.1"
                     /translation="MKRVMVILILLVTAFAFSWSGHDALTYIIVSQYEDDFDEFVQIT
                     PYTYADVDTQEYNPAIEGFYDYLGENYLPEEDLDLYTSIFPNPKPVDNLAPLWQILSV
                     YSYEPDLGMDKGLELKGIETLLGDSQGLRHMEYRMLFFIRIGKATDVVQYFSDLAEIA
                     YSRGDRYWAYRFMGRAIHYLEDVGQPFHTFPAPFFELVKLPFNIDKWQTVFANFHFAY
                     DFYGGYLLWEEYEPLVEAIKEVSPKEIKDPKQAAVSLRRYSRNKLSSVYYELKRTMGD
                     ELENPEAVWLGKAYFDDLQNAGETEKLDALSVEILRETASYVKGYINYMLARFAEIDS
                     ES"
     gene            23571..24794
                     /locus_tag="PKL93_06805"
     CDS             23571..24794
                     /locus_tag="PKL93_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNQ71004.1"
                     /translation="MGDNSKRVSRGLLDVRILRDVEFDFQLYRSLGAVSYEGGTVGEI
                     LSLVPHINCDDPDSWVKSFKELATKLKNHALIVLQKGNNESARQEFLRAANYFRAAEY
                     FGDPRDSKTRYLGMESRDCFVYAFRTTDIQFEAERIPYGEDFIPAYWIAPTTGGKKKT
                     IMMMSGFDGTSEEMYFQAGSAALQRGYAIVLFDGPGQVGMRRFSPETPLRPDYEVPIS
                     KVVDYALSKEDVDPSKLALYGISLGGYFSLRAAANDCRIRALISNSPITDMYKYMSAF
                     AGEAINSPEDITLETVDLVPTEYLSKAQKLQLVNIMLKYGRHSMFKAFERMKDFVVGD
                     SIKNIQVPFLAMVSEGEGEEALSQAKEASDSVSGKSTMRIFTREEGADSHCQVTNLPL
                     SNSVLLDWLDEVFGA"
     gene            24797..25486
                     /locus_tag="PKL93_06810"
     CDS             24797..25486
                     /locus_tag="PKL93_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNQ71005.1"
                     /translation="MKRIGLLGGMSWESSLEYYRLLNEMVKNRLGGLHSAECIMASVD
                     FGPVSDMMKTDDWKGIERVLSEAAQDLKRAGADFLIICTNTMHLIAREIEKASGLEVI
                     EIGIAVGEEIERRGLKTVGLLGTRFTMERAYYRDTLAGFGISVIVPSEEERAVVDRII
                     FEELCKGIFREESKSAYKKIISGLQMNGAEGVILGCTEIPLLIKESDVDIPVFDTTAI
                     HARAAVEHALS"
     gene            25731..27278
                     /locus_tag="PKL93_06815"
     CDS             25731..27278
                     /locus_tag="PKL93_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNQ71006.1"
                     /translation="MRKILSVLLSVLLLSAVLFSNVYSSASNGDLQGVKEALNSGGDI
                     NELGYANKSALMVAADSGHSDIAVFLIESGADISIVDDNGYTALHYAARKGLLEVVKI
                     LLERGADINAHPASSMFYEGYTPLILACDNSKNDVTLEVVKYLVEKGADLERVEYAFR
                     SPLIAAIFSKGTNTVKFLLESGADPNRPAKDGRTPLYLAISEGLPIEGIEALLEYGAD
                     VSVGNEYYTPLQLAVSKSKNDVAIKLIEFGADYWAVDEDGNTLLHRAAGKGSTKNIEM
                     FVKLGIDINARNNEGKTPLHIAAEDDHLKNVKLLISLGADVLARDNNNYLPFHLHLAV
                     YEEDVEVIEQLLSAGISVDNNEVETGISPLHIAAAYGKTDIAKLLIQKGADVSIKAQG
                     GLQPIHAAVGSYGSLEMVDYLLESGANIDAEDDEGRTPIFYAVVDYNMEAISHLGEKG
                     ANVNHRDHAGMTPAHYAVQDEWDGIEMLTILNSFGLLANVKDNSDKTPEDYAASEEIK
                     EFLKTLY"
     gene            complement(27383..27829)
                     /locus_tag="PKL93_06820"
     CDS             complement(27383..27829)
                     /locus_tag="PKL93_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNQ71007.1"
                     /translation="MSHDLSFSGEGYISRWIYCISRSANVYFSREMQKYGLGSGHFFF
                     LRVLMSKEGISQNELSDILGVDKATTAKAMSKLTEAGYVKREIDSLDTRVYKLFLTDN
                     GKKIGQKLRKLGSELEEILTEGFSGDEKQQLLSLLERAATNAKKAK"
     gene            complement(27833..>29063)
                     /locus_tag="PKL93_06825"
     CDS             complement(27833..>29063)
                     /locus_tag="PKL93_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ71008.1"
                     /translation="GVGSMGIAGISISLPVQIFVMAFAQMFGIGGASVISRSLGERDH
                     KKARRAAGNVMVFSVAFGLVMTLLGQFFLDQLLIMLGASEAIIPYAREYLGIILLGSA
                     FFSFGMAMNHVVRAEGKPKIAMAAMLISAVLNIILDPIFIFSLNLGIWGAALATVLSQ
                     AATSIYVLFYFLSGKSLLRVSLRSLIPEWRIMRETVSVGLSAFSRQVAGSLLAVVLNN
                     SLVFYGGDIAVAVYGVINRLLMVFIMPMFGVNQGFLPIVGYNYGARKMRRARESVKLA
                     SAVTTVIALFSSIVMFLFARQLISIFTDETELIESTIYALRIVILATPTIGVQVIASG
                     MFQALGKALPALFLSLLRQIIILIPLILVIPRFLGINGIWISFPLADLIAFAISLVFY
                     LRELKIFRSGELNVEEE"
BASE COUNT         8191 a         6232 c         7125 g         7515 t
ORIGIN      
        1 gaacggtatg aatacagaaa ggaccgcggt gactggataa gtgtaggtac tgacttgagt
       61 tatacgtgga ggggatactc agaaggaaat catgtcttcg aggttcgcgc tctggatgat
      121 ggaggcgcgt attcgcagat agttatctgg tcattcactt acagctatgc gaatcagccc
      181 ccggtgatta caaagactgg cggtcttgaa ggcgatatat acgtgtcaag caactcgttt
      241 tcatggacag gcagcgactc agatggcacg atagcaaagt atgaatacag gaaagacaac
      301 ggtgattggg tcgattttgg actcgggacg aactatacgt ggagtggtta ctcagaggga
      361 agccactcat tcagagtcag aggacgtgac gataggggag cgtattccga agaggctctt
      421 tggagtttta catacagtat tccgccgcag gagatggggg ccttcaaggt cgttaattca
      481 tggggagtag gtggctggga aaacgttcct gacggcttcc tgtacattac atacgaagca
      541 atgaaggaaa accaggtccg ctgcttcact atagacccaa gagaggacta cgaaccgaga
      601 gcaattgcag tattcgagat ttctcatgga ataagagatg attgcgagat aacggttggg
      661 atcggcaacc cttcttctcc cataagagaa aagagattcg atgactatag ctatagagga
      721 gggcagcatc ctttccctga caacaagatg gtgctggata tcaccgagct ccttcccttt
      781 gaagatgaga cattattcct gaaggtattt gattccttca gtgacttcac cacgggaagt
      841 atagagttct tttccgtgga agtattcgac aattatcaat ctggaactcc tgtggcgata
      901 tacacttcga ccgagacacc aaagaacacc gtaaataact cattcgtgaa cgtgcagatt
      961 cataatgttg ttgcagcaca gggctcttcg tattaccttt caagcataag gcaaggattg
     1021 tcaacggaaa tgctcgaact tctgaaagca gacctagggg tcctcgaaga agggagaaac
     1081 tacaacgaga ttattgacgg acacggtact ggtttgcgtc ctccttcaga agacgattgg
     1141 gatgaaatcg ccaggacctg gcatttgatg gatggcttct cagtccaggg aagcttacca
     1201 tcgactttag accattccgt ttcgatgtat tttcctcccg taggagatca gggcggcgaa
     1261 ggctcgtgcg tagccttttc aaatgggtat tatacttcca ctttctatga agccagggat
     1321 agaggctggg atctcagcgg cgcaagctgg acaaacggcg gagaacctac tccttcgtat
     1381 cagaacagga tattcagtcc tgactttatc tatcatcaga ttaatgatgg tgaggacggc
     1441 ggttcatctt accttgatgc tcagaagctg ttgtcgagag ttggagtcag cagctgggaa
     1501 aagatgcctt acgatacgag cgatcacact tcgtggcctt ccgaatctgc atggagagaa
     1561 gctccaagat acaggaacgg tatgaatgta attagctact tgacagtaag gacggatcag
     1621 gacatactga ctatcaagtc ctatctcgcc gcgggttatc tagtatctgt ttcaatagac
     1681 gccaatcagt acaagaacct tacagagaaa gacgtctgga acacatcaac ttacatttat
     1741 cccgatacta atcacgcaaa cacaatagtt ggttacgacg acaatttcaa cggctctctt
     1801 tgattaacgt cagcttctca gtttgcggca gtactgagca ttgaggatgt ctacagaagc
     1861 attctttctg tagaactgcg atactttcga attcacaggt tcttcggaat cagtattgct
     1921 tcgcgttagg tctacgattt gcctgtaaac gagtttgcgg agaagagcgt ggcaatgaga
     1981 ttgagtcaga gggtctggct tcatttgcta tcagcaaaag tttcttctgt agtttctttc
     2041 atgcgttgtt ttatctgttc acttctgcaa ggtattgctt cgagacgaat gtataatctg
     2101 ttcatacgat ggctttggca ggaatacagt tgctctccga tcaaagctct acagagccag
     2161 ggacatctcg gaggtgccgc atgaagcgag tcttggtgca cattttaggg tccgttagat
     2221 cttggctgct gataatgctt ctaatccctt ttggattact tttttcagag aacattgaaa
     2281 cacctgttga caattacaaa cctcccacag aggtttccgg gactatagcc gaggacacgg
     2341 tctggacaat cgatcgtagc ccgtacctgg ttagggcgac aataacgatt tctccaaatg
     2401 tgatcctcac aatagagccc ggcgtggaag tcctcatagt tcagaatcag gatttcatcg
     2461 ttaacggtac gctgagggcc gttggcaacg aggaaaaccc gattgttttc acaggtactg
     2521 ctcaggtacc aggttggtgg agatcgatca acattcaggg agaaggttcg gcatttcttg
     2581 agtggtgcga agtctccttt gcaggagcgt caacgggggc cggcgttctt aaagcgggat
     2641 cgggttctct gagactcgtc aattccgttg ttagaagggt tatgggagat ggccttcgga
     2701 tttcggcagg gtactcctcc tttgagagta tcaacaatac cttcatgtat aacacaaacg
     2761 gggttagggt cggaataaac gcatcttact ctgatcagag ttcgaccttt attttgaacc
     2821 aggtggacat ccatcttgac ggcggagcga tcacagggat tgtgaaatgg ggagccagtg
     2881 gtgactactc catgacggtt accggagatg tctctgtaaa cgcaggagca tcgctagaaa
     2941 tagctccggg aacggtggtc aaactgagac aaaacaacag gtttttggtt tacggtcaac
     3001 ttcaggcaag aggagaagaa gatcagcaga tctacttcac cgatttgcgc gatgatacgg
     3061 tcggagggga tgctaataga gatagcagtg agacgatcgc tgaagaggga tggtggagat
     3121 ctatcaacat ccagaatgaa ggctcggccg ttcttgaatg gtgtactctg gcctacggag
     3181 gtagatcgga tggtgcaact ctgctcaaga gcggaacggg ttctatcagg atatccaatt
     3241 cagcgattgt aagaagttcc ggggacgggc ttagggtagc tgcaggctac tctctatttg
     3301 aatcttcgga caactatttc gggtataact cggttggtat tcgattggga ataaattcat
     3361 ctttctctga tttcacttcc cgttttgaaa gtaacgatct cgatgttcat cttgacggcg
     3421 ggacgattag ctccagtgtg gtatggggtg caagcagcga gtattcaatg gtaacgggcg
     3481 gcgacgtagt tgtcgctgcg ggagcttcac tgaaagtcat gcccggaact gtaatcaagc
     3541 ttaggcagaa caacagaatc ctcgtctacg gtcatcttga ggcagccggt caagaaagcg
     3601 cccccatata cttcacagac tttcgaaatg atcttgtcgg aggggacgca aacagagacg
     3661 gcgaagccac cgttcctgca agcggctggt ggcgcagcat aagcctgctt acagaaggta
     3721 ccgcgaactt tgagcattgt acaatcgggt atcttggagt aagtgacatg gcaggagtga
     3781 taaagagcag cactggcacc ttttctattc tcaacagcac gattcacgac gttaccggtg
     3841 acggattgag gattgacaat tccgcaggaa gtgcggaagt gaagtattcg acactatctt
     3901 ataataccgg tgctggactg tactacaaaa ccgacggcgt ccacattgag gcgctgacca
     3961 tatcttccaa cggaattggg ataagactcc cggcgggctg ttcggtcgag gttgatgaac
     4021 agacctattt tgatgggaac gatattgcaa tacagatcga tcctgccgca atttcaggca
     4081 gagtaacgtg gggggcaccc agtaatatat ccatattgat gaacggcagt gtttcggttg
     4141 catcgggggc aagccttacc attaggcctg gcagcgtaat caagatagct cagaacgcat
     4201 tccttgcagt agatggagaa ctaatggcga gcggaaccga ggataacccg atctttttca
     4261 ctgagactcg tgacaactcg gttggagggg cgttgtcggg tgatggttct ttgccagaag
     4321 cgggctggtg gaggtctatc aacatacgta atgaaggtaa ggccattttc gactggtgca
     4381 caattgcata tgccggtcgt tccgacgggg ctgcgatatt gaagagcggt gcaggctctc
     4441 taagaatatc gaattctgtt ctgtcgcaca cttctggaga tggtctacgc ctatcttcgg
     4501 gttattcgtc ctttgagcac tttcagaacg agtattctta caacacaatt ggagtcaggt
     4561 tgggcataaa cgcttcattt gccgatgtaa caacaaggtt cgaaggcaac tccactgata
     4621 ttcaattgga cggtggcgcc ataaccggta cagtttcgtg gggatcggat ggcgattact
     4681 ccatggtaat aacaggcgat gtgagcatct cggccggtgc ctcgctaagt attcttccgg
     4741 gaactgtgat caaattcaga cagaacaaca gaatccttgt ttacggaagt ctccaggcat
     4801 tgggagaagc ggatttgcca atctacttca ccgacttgag ggatgatctt gttggtggag
     4861 acgcgaacag agatggtgga gaaactaccc ccgctcctgg ctggtggaga tcgataggta
     4921 ttatgactga cggcacggcg actttcgaac attgtgtgat tcgattcgcc ggatacggtg
     4981 atcgatctgg ggttctaaag agcagtactg gcgcggtttc gatgacagat acggtaattg
     5041 agaaggttgc aggggatggc ttcagagcag ataatgctgc cggaggagtg aagatcggca
     5101 gatcgacttt ctctgaaaac tccgaagccg gattgaactg caggacggac ggggtttcgg
     5161 tagaagaatc cgtcttccta tccaatgata ctggtgtaag gacagtcgca aacgcttctc
     5221 tgattttaga tgacgaaacc tacttctctg aaaacaacaa agacatttat gtagaagcgg
     5281 caactattag cggtagtgtt gtctggagag tccctaagca cctctcctta cttctcagcg
     5341 gcagcacctc aatctcagtg ggagcgagac ttgagatcaa gccgggaact gtagttaaga
     5401 tggctcaaaa ctctctgatc actgtcgatg gggagctcat cgcgcttgga actggagaag
     5461 aacccgtctt cttcactgat tatcgtgatg attccgcagg cggcgactca aatagagatg
     5521 cagatgcaac cttgccggtc aagggctggt ggaactctat aaatattaga gaatccggat
     5581 cggctgaatt ggacttcgtc gatatcggct attcgcagaa tggtattgtc agagccggtt
     5641 cgggatcatt cacactgacc aactccaata tccatgatgt ttctggagaa gcactcaagc
     5701 ttctttcggg atactcctat ctggagctgt caaacaacgt gttccttaga aatggcgctg
     5761 cagttcgatt ggctacaaac gtttctttcg atgacactct cgccgcattt gaagaaaacg
     5821 gtaccgatgt gtacgtagat ggaggcgaga taaacagtaa tgttgttttc ggcttgagta
     5881 gtgattattc attctacatc tctggagaac tgactctcac aaaaggggca gttctcgaga
     5941 taagacccgg aacggtccta aagtttgccg ccaatagggg actaaggatt tcgggaagtc
     6001 ttaagatgtt aggaactgaa caggcaccga taacacttac cgactggcgc gacgattcta
     6061 cgggaggaga cgcaaatcat gatggtgatg cctcgcttcc tgaacccggt tggtggaggt
     6121 cgatctacat acaggaagag ggcagcgcag aactcgaatg ggccagaata atgtatgcag
     6181 gctacggaga caaggcaggt gtcttcaaga caggttcagg atctctaatc ttaagaaatt
     6241 cagtgatctc tcgaattgcc ggtgatggtc ttctggtaag cagtagttcg ggccatctgg
     6301 aagtctccgg cttatcattc ctggaaaact cgacaggagt gggtatctca tctcagcaga
     6361 cagttcctct ggaatttgtt gattgcgtct tcgagggaaa cgtcgtttat ggtattagaa
     6421 acgatagccc ggttgaagcc gtcgccgtcg attgctggtg gggcgatata tcggggccgc
     6481 atcatccttc tctgaacaac cggggaacag gaaatcccgt tagcgacaat gtatcattcg
     6541 atccatggat aggaaaggaa gagctcatga atgagtccga aagtgaggaa gtgccctctg
     6601 agaatgctca agaggtagtt caagagaccg ttgttgcgga atggatagaa gtctcttcgg
     6661 acggactgtg ctatgaagta ccttcttcct ggttcgatga ttcggagggg tacagactcg
     6721 aactggaggg cgatgagaat tcgaagccgg taagcagatg gtttgatgct gaacccggct
     6781 cagagaacat cttcttcgtc atcgctaaag tgaaaccgga gttcctcgaa aaagaagaag
     6841 aggaaacaag actcaacatg gaagttgtcg aagaattcga aaggctaatc gccggggagc
     6901 cgggaatatg gatagagctt acggctgcgc aatggaatgt tgagagatct atctactctc
     6961 acgtctacga accgggaaac gatggatact gtctttcagt cggttttgct gcgaagacag
     7021 gcttctggac tcagtacgag cctgtactgg aaagaataat ggaaacgtta agatactgcg
     7081 gaaactgaat cggctatcag agcaagacgc gcaaaaacac cggcaaaggt ggaaatcttt
     7141 gccggtgctt tttacaaaag attactctac ctcgagaaat cacttctttg atgacggcgt
     7201 ctatcaatca agaagacatc acaatccttc atctgaactt gattcagaat ctcgattctt
     7261 cttatcgatc aactgagaac cgttcaacga agtatcagtt cttttgctct ttccgacccc
     7321 ttacccgcta accccaacca cggttcctca gaagctcgga tcccgaatca agtttggggt
     7381 agcgaaatcc aacagctcaa tcatcaaaga ccgaaaggtc atttctggag aatacattct
     7441 tctcagtacg gtccaggtat ttcagtctga agatgaagta tatcattccg attacttccc
     7501 agacgataaa gaacaggaag aaagacgtgc taaagtccac tccgacctgg tagagaagaa
     7561 taagcataat aagtattgaa aacaccacac cgagaatggg aatgaagatg aaccatttct
     7621 tcttaacaat tgcaggagcc gtttttctaa gcgtttcatt tttcttcagt ttgaaaaccg
     7681 caaacatcgt gggaataatc accgcgattc ctccaatcga agcgaaggct gcaaataggt
     7741 ttaggccgga ttcgccgact gaaatcagca taatggaaga gagaagccaa ataattgtca
     7801 gcaaaaccca tggtgttcct ctcttgttga ccagtccgag tttcgagggg aatactcgaa
     7861 gtttgcacag cgcaagcaaa gatctagtac cccacatgta tgtagcgttt atcgttgtgg
     7921 cgatagcgag tatcggaccg ccgaatgcaa agacgtagaa aagaaatccg ctaaggtaat
     7981 tcgaagctac atcattaaga gtgccgttag ccattacctc ttctcccact ccaaaagaga
     8041 ctattgccat caaaagatag cagatcagaa caattgaaag agataacata actgaaagcg
     8101 ggagattttt tctgggattc tccacttctc cgcccagctc tattacggca tttgaaccgg
     8161 cgaaagtaaa tgtaaggagc gcagaagcgt aaatcattgg acccaatccc gttgaaaaga
     8221 gtccttcaag gtttctcggg tcgatttttg caacgcccgg aacggtgtaa atggcaatcg
     8281 ctacaaagag gagagcaaca aatactatct ctactataga agccgttttc agcccgagca
     8341 gattgactac atagaagaat gtgagaatta tcaaagcgac aggtacgaca gaaattccat
     8401 tccaaattcc tttcatgtag gatgccagtg ccagagcatt caacggaaaa agtcccacaa
     8461 atgcaccgaa gagataggcc cagagaccaa ggaatgcaac tttgggtgaa aagaggaaag
     8521 cgccgtactt gaaggttcca ccggtcgtgg gaagtgtcga tccaagcaac atgacgttta
     8581 tcattattat cgccatagga agaaaggcaa agataaaggc aacgatggtc ccaatcccgg
     8641 cacttttgta agctatcggc atatagacaa atatgcctgc accaatagtc atacctactt
     8701 ctaaagcaac aagatcccag aacttgagtg atttcttcat ttgagacacc tttctggcca
     8761 aattagaagg cattcagaat ggcctgacag tatatggcgt attatacagt aaatcttctc
     8821 tatagaaaat tctcctatat ccatgacttc tgggggttcg ggtgacgatc taactctctc
     8881 cgctttttca gaaggggata ctatggcctg cgccggatac tcaacatctc ttgaaaggtc
     8941 gggcgtttct tcggaaacta aagatctttg ggttattgaa gtcaagaaat cgaaagcgta
     9001 cctgtcgcga atagttcaag agcgaaggtt tttacgtgtc cactaaatga aattggtggg
     9061 aagactagaa acctcaagta aactgttcaa cgcggttcta tgcatgaatt ttacggattg
     9121 tcatacgctt tcacaatttt gggcagcaga tacactgtct gtcagtcgtc atgttaggtg
     9181 gttctagatt gctgatccat atctcgaaaa agtcatgagt gaacgttaat tagcaggttt
     9241 ttctctgaga acccagtttc ccaattgaag cgttcctgta caggtcttgg agactactgt
     9301 gctacaatca gtttggatcc gttcttcgat aaatggagtg tgaaataaat gggcctttat
     9361 gcggtcatta ccgccgacat tattcagtcc agaaagcagg tgcttccatt tgagagaatt
     9421 gagcagactc ttgcttcctt cgcaggagag catctcgtca agtcgttcgc actgtcaaga
     9481 ggcgacgaaa ttcaaggcgt agtatctgat ctaagtgtca tcgtatcgct ggtgcgaagg
     9541 ttaagatacg ttatgtggcc tttagccctt agggtgggga tgagcatcgg cgaaatagaa
     9601 gatgacaaac ttaaaaaggc tggcacttcg tgggatttga gcggagaggt tttcttcagc
     9661 gcaagagacg ctcttgacat ggcaaagaaa tcaagggttt ctaacacgtt ctttctttgc
     9721 agtgatgaac ttttatccac tgcactgaac acgtctcttt ctctactgga gactattgaa
     9781 agcggctgga ctgaaaagca gtggcaggcc gttcatgtat atgaaaggga ggggacctac
     9841 gagaaggcgg ccagggttct gggagttact gctccggccg tgcagcagca ttgtgataaa
     9901 gctggctgga acgtggtcag ggtggcggaa aaggaactgg cgagattgat ctctctttcg
     9961 ttgaaaatat aaacctatat agatttatat gtgtacacta aagttaatta tctttatctc
    10021 agttaaataa agcggttaag tttaaccccg gtgtttatca tgattagtga gtatctttct
    10081 ggaactggat ggaggcaaat atgaatggat tcatgatacc tttagctctt tcgatcttgg
    10141 ctcatgtact cgccgatttc ttgttccaaa cagacaacac tgcaaaggaa aaaagcgagc
    10201 aacaggcaaa aggcttcctg aaacactgga tcgttgtttt cttgacgctt ttagtcttga
    10261 tgatgccctt cggactcatg gatgtgcttc tgtattgtgt agtcctttca ctgttccaca
    10321 tacttctgga cgtcttcaag tccagcattg aaagtaaacg tggcccggct accaggtttt
    10381 cgatgtttat tattgaccaa cttcttcatc taatgttgat cgtctttctt gttccgattt
    10441 ccagttttac aatagccaga agcttttctg cctttatagt ttggatagat tttcatacag
    10501 gactagattt agccgtactt ccggttagta aaatgctttc cgtacttatt gtctatctat
    10561 atgttctctt tgcgggagcg gtattcataa gaaagctttt cgacctgata tacaagaacc
    10621 agccggacta ccttgagaga atcgttggaa acagctcctc tctagataat gtgagaacgg
    10681 gaaaagcgat cggcatcttc gaaagacttc tagttctaac actttacctc acgggaaacg
    10741 tggcttccat aacaattgtc attgccgcga aatctctggc aagattcaag aacttcgata
    10801 acaaagactt tgccgagtac tatcttatcg ggacactggc tagcgtgatg attgcaatca
    10861 tcggtggcat gatcttggag gttctttgaa aacgttatgc gcaaagagtt tagtagtgtg
    10921 aggatagagg ttagaaaatc aagatcaaaa atcgtatctc gccgtgtttg agagaaagcc
    10981 gtccttggat aagatcaaaa ccagatccag aaacaagttc gggatgaccc tgaacaagag
    11041 catttcaggg caggctccgt ggaatgacaa agaatgctta ttccgaaagc gtctcaatct
    11101 gtcatgaact tgtttcagca tcccggttat gagagccgct gagcgcttaa aagcggagaa
    11161 cccgctgatc gctgtgaaga gccgtccttg gttcttcgtt gtttgttaga ataagagagg
    11221 caggggatgc aagataagtc cagtcctttg gataagatca aaagcaagat cccggatcaa
    11281 gtccgggatg acagagtgag aggctttcac aaagcgcccg gacaacttcc tcagttgcat
    11341 tgaaaccctg aactgattgc cggttctctt cacgagaccc cagaccctgt aatcgatacc
    11401 ccgcgctccg cgtcatctga acttgtttca gcatctaggt ctagcgttca ggttttggtt
    11461 cttattcttg caacttggaa cttgcatctt gcaactaatt tcagctcttt cgaacctgct
    11521 atcccagacg ccggattatt gaaaagagag gactgatttc atttcatcac ccaactgatg
    11581 gtcgaatgtt ggatccgtta taggagaatt ctacacgact cgcatatctt tgtttacagt
    11641 gaggaagaga agaatagata atgctagaaa tgtagaagcg acaatgaaga tctcttctga
    11701 atgaacgacc cagaagatcc actaaaactt cagcatggga aggtcattga gttactgccg
    11761 atcttcatat caaagatgat agactttata gtaaacattg tctcacactg gagtatttat
    11821 gaagaaccgg ctaatctctc ttttcctagc tgcaattgct acagttttcg ccgctgtcat
    11881 aattttcgcg atgacagaag taaggtattc caaaatagag aatcacatcg caaactccgc
    11941 caggataatc acggagaata acgcaaagac cgccttcctc ggttacttcc actgggattc
    12001 gatgtacgag gctttggagt caggtgatgc cgacttcgtt atctacaatc tagaagatct
    12061 gctgaagaac aatagcctga tcgagggtgt cgttgtcgtg taccgcaacg aggaattcgt
    12121 atctgccggt gaaagcttcc agactcttag agatcacacg attggagctt cggccttcta
    12181 cgagatcatt ccaagtggag aggacttatt ggcgacgatg agagttaccg atagcgccgg
    12241 cttggagagt ctgaaagact catatgctat tctgaaactt aatctagacg agattctaag
    12301 attattgact actgaagact acagattatc gagtgtagaa gggacttccg tgttcgaagg
    12361 tatctcactt tctattatca gaaagtggtc tcccgccaat tatgctactg tgagtctcgt
    12421 tttcctgatg tcgctttcat tcactatgat tgttcagcgt ttggaaggca aaaggtctat
    12481 agcatccaag aacaacgaga tgctgttgct tctaaaccac atcccaacac ttatctggta
    12541 cttcaaagat tccgagactt tcgggatcgt aaacaagtca tttgcggagt tctttggcat
    12601 gcgtccggaa gatatcgaag gaaggaagat tcgcgagatc ttttcgggag aagacctctc
    12661 aacaagtatt gaaaccaata aactagtctt tgctggcaaa gaggagcaca tttacgagca
    12721 agataccagg aattcgaaag gcgagcttag aactctgatt gttaccaaga ctccggaaat
    12781 cgatgaaaac ggtaatataa aatccgtagt ctgctctgca tgtgacgtaa cggatgaacg
    12841 caaggctctc agaagaatag agctcatcca gttcgggctc gacaacgcaa acgatgaggc
    12901 tttctggatt gcccctgacg gatcgattct ctacgcaaac agcgctgctt gcaagagtct
    12961 cgggtattcg agagaagaga taactgcaat gaaggtcaat gatcttgaca aatctctaga
    13021 agctgatgac agaaaaagca gctgggagag gctcaaatca aacgggaaag acaatttcga
    13081 ggcataccat ctgagaaagg atggttccac tttccccgtt gaaatcaaca gaaactattt
    13141 taagtacgat ggaagagaat atgaattcac gtttgcgcgg gatatttctg accgcttaaa
    13201 aagttttgag attcttgaaa gagacaagtt caggatcgaa cggctccacg aggcggctct
    13261 caatctcgaa aggtgcagtg aactgcagag cgtctacgac cttgtcatcg aagcggcaaa
    13321 agagattctc gaattcgaca tttgcttcat atgcgtaagg gaagaagaaa atctcgtaat
    13381 aaaggcgtct tccaacctga atccacggga tcccctggaa atgtcgatcg ataagggcat
    13441 tgtaggaagg acttatagag agaagaagtc atatgtaatc gatgatatcc aggagtttcc
    13501 cggtgctctt agaacaaacg atatatatag agctggactc agtgtaccgg ttggagatgt
    13561 cggagtcttt caggcaatgt ccgtgaaaaa ggcgatcttt ggcgaacagg aattgcgtct
    13621 tgctgaactc ctgatgtctc atgcccgcga agcgattgtg aggattgaaa cagagaagcg
    13681 catgaattac atgtctcttc acgataaact taccgacctt tacaacaggt tgtacttcga
    13741 ggaagagctt aaaagacttg aaggctctag attctacccc atctcaattg tttcggcaga
    13801 tatcgatgga ctgaagttga tcaacgatac gatgggtcac tcaagaggag accagatact
    13861 catcgagctt tcgcagatat tgagatcttg cttcagaaag acggatgttg tggcgagatt
    13921 tgggggagat gaattcgcaa taatattgat caagacggac gaggagatta cagaaaagat
    13981 cgctctgcgg gtaagaaaga ttgtggaaaa attcaacatg aacaactccg gaccgcctct
    14041 aagtgtttcg atgggtattg ctacgagcaa tggccctgaa caagaacttg ttgaaacact
    14101 taagcaagcc gatgacttga tgtaccgcga taaactttac agaagttcga gcgttagaag
    14161 tcagatggtc aacacactcc ttgtaactct agccgaaaaa gaccagatct caggagggca
    14221 tgcgaagaga cttcagaaac tctgccttga acttggaaga agagccagac ttagcacgag
    14281 gcagttgagc gaccttgcat tgttcgcaca ggtgcacgat ctcggaaagg tgggaattcc
    14341 agacagcatt ctgtttaaac caggtccttt gaccgctgat gaatggagga taatgaagct
    14401 acatcctgaa aagggatata ggatagcgat ctcatctccc gacctctctt cagttgcgga
    14461 tttgattctc aagcatcacg aaagatggga tggaaggggt tacccgctgg gcataagcgg
    14521 tgaagacatt ccaatagaat gccggattct ctcgattgtc gatgcctttg atgctatgac
    14581 aaacgacagg ccttacagta gggcgataag caaagaagag gcagttgaag aaatagtgaa
    14641 ctgctcaggt acccagtttg acccgaaact ggtccaaatg tttaagagta tcgtgagcaa
    14701 tgaacttgtt tagatctcgt tgattaccag cttcaccttt ttgtagctga ggatagaaat
    14761 cacgatcgct actgcaagtg atgctagaga aatcagtatc agccagatat ttgaattgct
    14821 ctctgaagcg ccactcagta ttgttgttag tggcattatt gcaccaaagc tgaggcccat
    14881 gcagatcacc tggttcagaa ggacaaactt tgttctcata aaagtattcg gattatccat
    14941 ctgatccggt ggggatacca ggacacccct gattcctaat agagaagagg taatatatag
    15001 gacgaagact atgggaacta caacaaaata gccgaatgac tcgcccttca cgagtatgct
    15061 ggttgtgaga accccgacga agagaactga ccccagcaga acgggcaagg ttgccttcag
    15121 ggctatcatt cttcccagag agatgggaag tgtctttgtc gtttcaatga aagggaactc
    15181 tcttcccata agaaatgcag attgaattgt tgtaaaaata gtgcttatga gcaccgtagt
    15241 aaatatcgaa gcaacagcgt catctgaaat gagtccgaaa atcagaccga acactacggg
    15301 gtagaaaaga tagtatataa gttgctcata acgcttatag atttttagct ctcttctcag
    15361 caacacccct cgcgatctcc cattaaagga gaccgttttc gttctgcttt ttgtatagga
    15421 aaccggttca aacttaatag atgaagctac tctgaaaaat atgtatccca aaaccggaat
    15481 tgccccaagg cctacaagaa gataaatcgg ctgctccgaa gcttttacac tccaggtaaa
    15541 aacgttcatg ggattcgaaa gaactcccca tgcggatgcc agcttctgca agtacagcgg
    15601 tatattgtcc gtgcttctcg gaaccagctg aacgataata aaaaacatga aaccagcgat
    15661 tatctgaaca acaaacatca cttttcgcgc tgccgccctt gacatgaact ttccgaaagc
    15721 cactgaaact atggaggcca agagcacagt gtctattact accagaacga gcgcgggaat
    15781 cccaatccat ggactgggat cgactctcag atgaaaagga agagctattc cgatgaagac
    15841 ggataaggta agaccctgat atacagtcga tatcattatt tgataggcta ttatgctgac
    15901 tcttttcacg ggaagaacga gaagcatatc tatctcctca tttctcatga agagatatga
    15961 tgacgtcgcg gagaaagaaa tcaggaagaa gaaccctgag atagttagga agaaacctgc
    16021 cataaccttt ccaagagaga ctccctcgat agagacccct gcagtagatc catatacctc
    16081 ccaaagaaac gtgccgatca taatcgcaaa tacggcgaaa ataaatagct gaccggaaag
    16141 gccaccccta ttgcctctct tgcctgggaa gagaaacagc ttgtgtttga agaatagctt
    16201 taaatcattc aaaggggcca cctcacagtg cacttactat ttcctggatg tcttcatcct
    16261 caccggttaa ctgaaggaag aggttttcga gagtttccct ttcatcggca ccggccttcc
    16321 ttctcaattc attcattgta ccttccgaaa tcaacttccc cttattgatt attcctatcc
    16381 tgtcacacat cttttcagcg atttcaagta cgtgggtcgt cataaaaatc gtagatccct
    16441 cattcttgta cttctccaga agcatcttca ggatttttgc gcttcttgca tcgaggccga
    16501 ctgtgggttc gtccaggaat atcacttcgg gccgtctcat cagaacggat attaccatta
    16561 ttttctgttt cataccgtgt gacatctcgg aaatcatctt acctagataa tcgattccaa
    16621 aggcttcgca taactcgcca attctcttct tcgcctcttg cttcttgtcc gggaagacgt
    16681 ccattatgaa ctccagaaac tccgcgcctg tgaagtaaga gtagattctt ggctcatctg
    16741 gtacaacgcc aatcctgctt ttgataagca gttcgtttct gaagaagtcc atatctagta
    16801 ttcttacctc tcccgatgtc ggcctcaaag tgcctgtcaa cattcttatc gtagtcgttt
    16861 ttccggcccc gttcggtcca agaaagccgt atatctcccc tgaatttatc gacagatcta
    16921 tctcatttac tacggggtct ttgtcgaaga ctttagtaag cttattacag ctgatcattt
    16981 ggatccctcc agactatatt gctaagtgtc ggccagattg ttctcacgcc ccaattttac
    17041 gacaagagtc aaggcatttc aaattacgat accaattcat ctgtgaagcg ttcaatctcc
    17101 acaaggggcg atgaatcgta aagtcagatg atataattgg ctgagttcat gaggtgcgaa
    17161 tatgagaaga gcgttatcct tcgccatact ggccgtagta gttgtggttt tgttgtttgg
    17221 aataaacggg tttagctttg atccctcccc ggctgccttt ttgagcgatg acgattcgga
    17281 gcttggggca ttcaacaaga tcgcggaagt tttcggtgat tccggatcga ttgttttggt
    17341 tcttgaagct tctcaaacct ctttggagct cttgaaaagc atcactgaaa agattaattc
    17401 tcttgaatgg gtgaagtccg ttttgtctcc tacagaggca gttaagctgg gaagcttcaa
    17461 tctttttacg atgagtattc catcggaaag ctacgtttat gaagatgagg gccgactggt
    17521 tcttaatgaa gaactgttca cggatcccgt ttacagcaat ctgatcgttt cttcggatgg
    17581 taggtactat gcgatcatga ttaccatagc agaggggaac gagctgaaaa gtgacatgat
    17641 gatcccggaa ctgcgttcgg tactggactc ttccggcgta gaaagctaca aattgatcgg
    17701 agaaagtgtt gctaatttcg aaaccttcag gtcgatcttt gatctaacct tcaagtatcc
    17761 acccttcata ctgcttgcca tcatggcagt ttacatgatc aagtccagaa gaatatcgct
    17821 atcacttctg acccttgtcc caccgttggc tgcagtaatg gtgataatag gaataatggg
    17881 actgctcaaa ctttctataa acagtcttac ggttatgata ccttcgttca taattataat
    17941 tggcagtgct tacgggatgc atttcctatc gagatttgaa gaaaacatcc atatgaagaa
    18001 tgccgtcaga aggaccatcc acgaagagag agttcctata ctcttttctg ctctaacaac
    18061 tatggcgggc ttttcttcat acatcctact cgatatgaag gcctttcagg aaatggggat
    18121 attcgtctgc tccggaatct ttttatcggc gatttttact atcactgtcc ttccggggct
    18181 tgttacccct aaggtgggta ctaaacgcga ggttttgcca ccgagggatg ttcatccatt
    18241 agttaaaaga gcggttatct gggttgtcgt tgccacaagt attgtctctc ccttactaat
    18301 catcactatt ccaatgacga ttgatcagta taattttttc aaggaggatt cagagatcag
    18361 agaaagcgca agaacgatga aggaagcctt cggttggttg acgaattata ctctcatggt
    18421 cgaacctgca gagggaggga gcatttcatt cacaggtgac caggttgaga atctagaagc
    18481 tttcgaacgg gagctgaagg ggattgaagg agtttcaaag gttttgagtc tctttgacct
    18541 ttcgagagaa accaacgtac ccttaccttt gttgatcaga gtgctcaact ctacagaggc
    18601 atttggtgat tctacttcac ttctggtcag tgacaatgcg atccgtttca acatcttttc
    18661 atctcagagc gacagcctga gtgcggagag attgaaagcc gcagtcgaaa aggccataag
    18721 aaaattccct gaaatgaaca agaacttcga attcactctg gctggaacaa ctctcatatg
    18781 ggagagcgtc aattcatcgg tagtcaagaa ccagattcaa agtctcattg tctcattcgg
    18841 gcttatcatt ttactcctgt tttctatatt caggagcttg aagtcaacca taatagctac
    18901 tattccaatt ctgctgacgg cactgttcaa cttcgtcttc atggccgttt tcaggattcc
    18961 cctgagcatt tctacggctc ttatttcggg aatgttgatg ggactggtaa tcgactacgc
    19021 catccatttc acgatctggt ttaggagatt tggtgattcg cataaagctt atgaacaaac
    19081 ggctagggca attcttacaa atggcataag tcttgttgcg ggtttctccg ttttgctcct
    19141 gacaccatta ctgctttatg tggatgtagc gaaacttatg gtaagcgggc tagctgtggg
    19201 gatggttctt accctaatac ttctccccga aatcgcatcg aggagtgaaa ggtttttgag
    19261 cagggccaaa gactcagact gatataattc ttataataat caaatggagg tgcgcggttc
    19321 tgaaagcact tgtttctctt ctcccagagg atttggatcg tttcgtcaga gagatatggg
    19381 atgaattgaa gaccgagttt ggtttttatt cagtttttct aacccctttt cctcatataa
    19441 cctactgcat tgtagaagag gaggagactg gaatcgaccc tttgatcgag gtcatagctt
    19501 ccagtacgcc ccctttgatg gccagaacgg agtatctagg agtcttctcc ggtgagaaac
    19561 ccgtgatctt cattggactc gcaaggaatt cgcgtttagc atctctccac catgctttat
    19621 ggatcaaact ccgtgattcg gtaagcaaca gagcgatcgc ttatgatgaa gaccactgga
    19681 tacctcatat taccgtcgtt tttggagatg atcttaaacg atccaacatt ggtcctgtta
    19741 tggagagact tgtcttcagg gattttcatc acgagtttac aatagacaat ctgaccatac
    19801 tcgaagaggt tcccgacgaa gggatagttg tcaggaagaa gctgaagctc gtcggccagt
    19861 cgaagaaaga ttgacctgta tctggctctt acaatccctt ctcaaagggt aaacaactag
    19921 aaatcaagct accagattac ctgtttgtta taatctatcc aaagaaggag gtagatgcat
    19981 gagaaaagtt gcctggaagg acatagatct gaagattgcg ctaccggaag acgtgaaatc
    20041 tactgagtgc attggcgaac tggaagagtt tataggtcag gaaagagcca taagggcact
    20101 tgagactgga cttcacatag atgcgaaggg ttacaatgtg ttcgtttcgg gtactaccaa
    20161 cactggcaga aggacctttg taagtcgcta tctcaaaaag aaagtcgagg gcactaagac
    20221 ccccggagac tggatatacg tctataattt tgacgaccca aggtctccaa actcaatatc
    20281 ccttgaagcg gggacgggaa agatatttca gaaggaaatg aatgactttg tcgagatagc
    20341 tataaactcc attggcgaga gctttcaaag cgaggactat cagcagaaag ttacatcctt
    20401 acagaatgaa cagtcggaga aacgttcaaa catgttgaag gaactcattg agaaggccaa
    20461 agagaaagac tacaccgttc aaataaacca gaccggagtc gcaacaattc ctatatggaa
    20521 cggaaagcca cttacacagg aagtatatga ggctcttccc gaagattatc agaagcagat
    20581 tacgaaaaag ggcgaagagg ttcgtgagct ggttaattca tatcttctga aactgagcaa
    20641 gatggaaaaa gacttcggcg aaaagtataa ggaactgaac agaaaagttg cctcatttgc
    20701 cgttgaaggt cacataaggg agatgaagga tagattcagc gaaagtaaag aggttgtgga
    20761 ctttattgag agcatgaaga aggatcttct tgacaatctt ggagtcttct tcagccagga
    20821 gatcgattcg aaggcctttt ttggcaagag atacgcagta aacctatttg tcgacaattc
    20881 tggaatagag ggaaaaccgg ttgtagaggt aacaaacgcc aattactctt ccctctttgg
    20941 aagaatcgag tacgtagcga agatgggaat gctcgatacc gatcacacaa tgataagacc
    21001 aggagcgata cacagttcca atggcggata ccttgttcta gatgctaaaa acgtgctaag
    21061 tgaaccgtat gtttggcaaa cactgaaaca ggttctcttt tcgggcctgg agggaatcga
    21121 gaatcttgaa cataagatcg gcctgctttc gactgtgagt ctgaagccgg agccgattcc
    21181 acttgacata aagatcgtca tgatcggaga gccctggata tacagtatgc ttagcaccct
    21241 tgacaccgat tttaagaagc tcttcaaagt aaaggcagag ttcgactggg aaatgactat
    21301 acagaatgat actgtggaca agctctgcgg actcatgtgc aatattgtta aggcgaactc
    21361 cttaaggcca ttagagagag aaggaataat agaggtcatc aaaagagcga tcttcctttc
    21421 tgggagtaga aagaaggttt ctacacagtt cggtgcactt gaacagatcc ttctcgaaag
    21481 ctcggccgtc gcggaagtca gtggtcacga gatgatacag gccgatgacg tatcgactgc
    21541 atgggaagaa atgcacaaaa gggtttcgct ctatcaagat aagattaaag agatgttcca
    21601 gaactcgact ttaatggttc aaacagaggg ggaacttgtc ggcgaaataa acggcttgac
    21661 agtgatccag actgaagatc tttctttcgg aataccggtg aagattaccg ccaaagtcgg
    21721 acctggcaac agcggaatcg tcgatataca gaaggaatcc gagcttagtg gacatattca
    21781 caataaggcc agtctgacca ttcaaggata tatgagctcg aaatacgcgc agcagttccc
    21841 gctaagccta aatggctcga taagtttcga gcaggtttac tcggcggttg aaggagatag
    21901 cgcttcggtt gccgaaacga tagccttcct ttctgcaatt gctgaagttc caataaaaca
    21961 gtcgatagcc gtaacaggtt caataaacca gagcggacga gttcagccgg taggtggtgt
    22021 ccaatacaag attcagggtt tttatgatct ctgcaaaatt aagggcttca acggccagca
    22081 gggagtgata gtgcccgaag cgaactccga taacgtgatt ctaaacgacg aaataatagg
    22141 agcgattaaa gaaggtagat tcaatgtctg gactatcgaa accgtggacg aggctataga
    22201 aattctcacg ggaatgaaac cggggaaaat cggaaagaac gggcagtact cttcaggcag
    22261 tttcaatcga cttgtcgttg acaggctcaa gaagttctac gagattgcct ctcgaacaca
    22321 tgatcgaagc ggcaaggacg ccgattagtc cggccacttc attaaaccag caaaaacggc
    22381 gatagagtat acctctgact atcgccgttt tgattttaag aacagcgctt ctaggattcg
    22441 gaatctatct cggcgaatct cgcaagcata tagtttatgt aaccctttac ataggaggca
    22501 gtttctctta aaatctctac tgaaagcgca tcgagcttct ctgtttcgcc tgcgttttga
    22561 agatcgtcga agtaagcttt tcctagccag actgcctcgg gattttccag ttcgtccccc
    22621 attgtacgtt taagttcgta atatactgaa ctcagcttat ttctggagta tcttcgcaaa
    22681 ctaacggccg cttgcttcgg atccttaatc tctttgggtg aaacctcttt gatcgcctca
    22741 acgagcggtt cgtattcttc ccagagcagg tatcctccgt agaaatcata tgcgaaatga
    22801 aagtttgcaa aaacagtctg ccacttgtct atattgaatg gtagcttcac cagctcgaaa
    22861 aacggtgccg ggaaagtatg aaatggttga ccgacgtctt caagatagtg aatggctctt
    22921 cccataaacc ggtatgccca gtaacggtct cctctcgaat aagcaatctc ggcgagatca
    22981 ctgaaatact gaaccacgtc ggtcgcttta ccaattctaa tgaagaaaag cattctgtat
    23041 tccatgtgtc tcaacccctg gctgtctccg agaagtgtct ctattccttt aagttcaagc
    23101 cccttgtcca tacctagatc gggctcgtaa gaatagacag aaaggatctg ccacaggggc
    23161 gccaagttgt caacgggttt ggggtttggg aagatcgagg tgtaaagatc gaggtcttct
    23221 tcaggcagat agttttcacc gagataatcg tagaaaccct ctatggccgg attgtactcc
    23281 tgagtatcta catcggcgta cgtataaggg gtaatttgga caaactcatc gaaatcatcc
    23341 tcatactgcg aaacaatgat ataagtaagc gcgtcatgcc ccgaccacga aaaggcaaac
    23401 gcagtcacaa gcagtataag aatcaccatc actcttttca tgtgatcacc tccagtctga
    23461 taatatcaga tttcatgttg cattattacc ttctcaagag cgtttcgctt tcggtattag
    23521 aactcttttc tgatagtatt attgagaaaa ggcgttaaag gaggtgcgaa atgggggaca
    23581 attcaaagag agtctcaaga ggtttgctag atgtgagaat actgcgtgat gtcgaattcg
    23641 attttcaact ttacaggtct ctcggagcgg tttcctacga agggggaacg gttggggaga
    23701 tcctctctct tgtaccgcat ataaattgcg acgatcccga ctcgtgggtg aagtcattca
    23761 aagagctcgc gacgaagctg aagaatcacg cccttatagt tcttcagaaa ggcaataacg
    23821 agagcgcgag acaggagttt ttgagggctg caaattattt cagagctgcc gagtattttg
    23881 gcgatcccag agactcgaag acaagatacc tcggaatgga aagcagagat tgctttgtat
    23941 atgctttcag gaccaccgat attcaatttg aagcggagag gataccatac ggcgaggatt
    24001 ttattccggc gtactggatt gctccgacaa caggcggcaa gaagaagacg ataatgatga
    24061 tgagtggttt tgacgggacg tcggaagaaa tgtatttcca ggcgggctcg gccgctttgc
    24121 aaaggggtta cgcgattgtt ctcttcgatg gtcccggtca ggtgggaatg agaaggttct
    24181 cgccagagac tccgttgcgt cccgactacg aagtcccgat ctccaaagtt gtcgattatg
    24241 ccctgtcgaa ggaggatgtc gacccttcta aacttgcgct gtacggcata agtctgggag
    24301 gatacttctc gcttagggcg gcggcaaacg attgcagaat aagggccctg atttctaact
    24361 cgccaattac tgatatgtac aagtacatgt ctgcatttgc cggagaagcg atcaattctc
    24421 ccgaagacat tacacttgag acagtcgatc ttgtgccgac cgagtatcta tcgaaggccc
    24481 aaaaactgca gctcgttaac ataatgctta aatatggaag gcattcaatg ttcaaggctt
    24541 tcgagagaat gaaggacttt gtggtcggtg atagcataaa gaatatccag gttcccttcc
    24601 ttgcaatggt tagtgaggga gaaggagaag aggcgctttc ccaggcgaag gaagcttctg
    24661 atagcgtttc gggaaagtca acaatgagga tcttcactag agaagaaggc gcagattccc
    24721 actgtcaggt tacaaatctt cccttgtcca attcagttct tcttgactgg ttggatgagg
    24781 tcttcggagc gtagacatga agcgcatagg tttacttgga ggaatgagct gggagtcatc
    24841 gctcgagtac tacagattgc ttaatgagat ggttaagaac aggcttgggg gattgcattc
    24901 cgcggaatgc ataatggctt ccgttgactt cggaccggtc tcggacatga tgaagacgga
    24961 tgactggaag ggtatagaga gagtcctttc cgaggctgcc caagatctta agagagcggg
    25021 tgcggatttt ctgattatct gtacgaacac aatgcacctc atagcaaggg aaattgaaaa
    25081 ggcatccgga ctcgaggtca tcgaaatagg cattgctgtc ggtgaggaga tcgagcgaag
    25141 aggacttaaa accgttggac tcctcggaac aagattcacc atggaaagag cttactatag
    25201 ggatacattg gcgggatttg ggataagtgt tatcgttccc tcggaagaag aaagggcagt
    25261 agtagatagg atcatcttcg aggagctgtg caaaggaatc tttagagaag aatcaaagtc
    25321 ggcatataag aaaataatta gcgggttgca aatgaacgga gcagagggag tgatccttgg
    25381 ttgcaccgaa atacctcttc tgataaaaga gtccgacgtg gacattcccg ttttcgatac
    25441 gacggccatt cacgcccggg cggccgttga acatgcactt tcgtgattcg agactgcgaa
    25501 tagtagatcg ttcacagttc cacagaaaag tatactcaag tgtccgcttc ataaaacaag
    25561 ggcagtatgt ttctgaacca gttagttcaa cctgatgtta gattcaagat cactcttgta
    25621 tggatctcga caggttaatg gaatatttca gctggatact gtcacacgcg gagatcatta
    25681 gctatttcaa ataactgaaa ttgcctacat ccatgaagga ggattcaagt atgagaaaaa
    25741 tcttgtcagt gcttttgtct gtattgctgt tatcagcagt cttattttca aacgtttaca
    25801 gcagtgcgtc caacggtgac ttacagggag taaaggaagc cttgaacagc ggtggtgaca
    25861 tcaatgagtt gggttacgca aacaaatcgg cccttatggt tgctgcagat tccggccact
    25921 cggatattgc ggtttttcta atcgaatccg gagcagacat ctccattgtt gacgacaacg
    25981 gttataccgc tcttcattac gcggcgagaa agggcctcct cgaggttgtg aagatactgc
    26041 ttgaaagagg ggcggatata aacgctcatc cagcaagttc gatgttttat gaaggctata
    26101 cgcccttgat cctcgcttgt gacaactcca agaacgacgt tactctggaa gttgtgaagt
    26161 atcttgtaga gaagggagcc gatcttgaga gagttgaata tgctttccgg tctccgctga
    26221 tcgccgcgat cttttcgaag ggaacaaaca ccgtaaaatt ccttctggaa agcggcgccg
    26281 atccaaaccg accggccaaa gacggcagaa cgccgctata cctagctata tcggaaggcc
    26341 tcccaattga gggcatagag gctcttctgg aatacggggc cgatgtgtct gtaggaaacg
    26401 agtactacac gccgcttcag ctggctgtat ctaagtcgaa aaacgatgtc gctattaagc
    26461 tgattgagtt tggagcggac tactgggctg tagatgagga tgggaatact ctcctccatc
    26521 gtgctgccgg aaaaggttcc acaaaaaaca tagaaatgtt tgtcaagctt gggatcgata
    26581 taaacgctag aaacaacgag ggaaagacac ctctccatat cgctgccgaa gatgatcacc
    26641 tcaagaatgt caagctgttg atctctcttg gagcggacgt cctggcaagg gacaacaaca
    26701 actatttacc gttccatctg catctagccg tgtatgaaga agatgtagaa gttatcgagc
    26761 agttgctttc ggcaggaatc agcgtagaca acaacgaagt cgagaccggg atctctccgc
    26821 ttcatatcgc tgcggcatat ggaaaaactg acattgcaaa gcttttgatc cagaaaggag
    26881 cagacgtctc gataaaagca cagggaggtc ttcagccaat ccatgcagcg gtgggtagtt
    26941 atgggagtct ggagatggtt gattacttgc tggaaagtgg ggcaaatatc gacgccgaag
    27001 atgacgaagg cagaacccca attttctatg ctgtagtaga ctataacatg gaggcgatct
    27061 cccatctcgg agagaagggg gccaacgtaa atcataggga ccatgccggt atgacacccg
    27121 ctcattatgc ggttcaggac gaatgggacg gaattgaaat gttgacaatt ctaaacagtt
    27181 tcggccttct agcaaatgtc aaggacaact ccgataagac cccagaggat tacgcggctt
    27241 ccgaagaaat aaaagagttc ttgaagactc tttactgaag attcagataa gcttttcgca
    27301 atgtcccaca aacatggcct ctgcgtttgc ggagaccgtg ttttttgatt gcctgagaag
    27361 ggacccggag atagttatga acttactttg cctttttcgc attagtggct gctctctcaa
    27421 gaagggatag cagttgctgc ttttcgtcgc ctgaaaagcc ttccgttagt atctcttcaa
    27481 gctccgagcc aagttttcgg agtttctgtc cgatcttctt cccgttatcg gtaagaaaaa
    27541 gcttgtaaac ccgggtgtcc aaagaatcga tttctctctt aacgtaaccc gcttcagtta
    27601 gtttgctcat agcctttgcg gtcgtggcct tgtcaacgcc aagaatatcg ctaagttcgt
    27661 tctggctgat tccttctttt gacataagaa ctctcaaaaa gaagaagtgc ccgcttccca
    27721 atccatattt ctgcatttcc ctgctgaagt agacgtttgc actgcgtgaa atgcagtata
    27781 tccagcgact tatgtaccct tccccactga aagatagatc atgagacatc cgttattcct
    27841 cctcaacatt gagctcgcct gaacggaata ttttcaactc tctaaggtag aaaaccagcg
    27901 agattgcgaa agcaatcaaa tcggcaagag ggaaggatat ccagatgccg ttaatcccaa
    27961 ggaagcgcgg gataactaaa atcagaggga taagtatgat tatctgcctg aggagggata
    28021 agaaaagtgc tggtaaagcc tttccgagag cctggaacat tccagatgca ataacctgaa
    28081 ctcctatcgt gggggtggca aggataacta ttctgagtgc ataaatggtg gactcaataa
    28141 gctctgtttc atcggtgaaa attgatatga gttgtctcgc gaacagaaac atgactattg
    28201 acgagaaaag agcaatcact gtagtaactg cagatgcaag tttgacagac tctctcgccc
    28261 gtctcatttt tctggcgcca tagttgtatc cgacaattgg aagaaatccc tggtttacgc
    28321 cgaacatggg catgataaaa accatcagaa gtcggttgat cactccataa actgcaacgg
    28381 caatatctcc tccgtagaaa accagcgaat tgttgagaac cacggccaga aggctgccgg
    28441 ctacctgtct tgaaaaggcg gagagtccta ccgagacagt ctccctcatg attctccatt
    28501 cggggatcag cgatcttaaa gagactctca gcaagctttt cccacttaag aagtagaaga
    28561 gaacatagat tgaagtggca gcttgagaca gaacggtcgc caaagccgcg ccccaaatcc
    28621 ccaaatttag agagaaaata aaaatggggt caagtataat attgagaacg gcagagatca
    28681 acattgcggc catcgctatt ttcggttttc cttcagccct caccacatga ttcatggcca
    28741 taccgaaaga aaagaaagcg cttccaagaa gaattatacc caggtattct cttgcatacg
    28801 gaatgatggc ttcactagca ccgagcatta taagcagttg atctaaaaag aactgtccta
    28861 ggagagtcat taccaatccg aaggcgaccg agaaaaccat tacgtttccg gcggctcgtc
    28921 tcgccttttt gtggtccctt tcacccagcg acctcgatat cacggaagcg ccaccgattc
    28981 cgaacatttg agcgaatgcc ataacgaata tttgaacagg caaggagatc gagatgcctg
    29041 cgattcccat cgatccgaca cct
//
LOCUS       DAOQYI010000021        29032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1888_length_29032_cov_72.680816, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000021 DAOQYI010000000
VERSION     DAOQYI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 29032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29032
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1888_length_29032_cov_72.680816"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PKL93_06830"
     CDS             <1..595
                     /locus_tag="PKL93_06830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ71009.1"
                     /translation="PFTIVIYSVEISERLCYFISLRDNSESTVLRNILRDIIYIDPLS
                     GFGNERSLNRFLSKKISSSKENGACFSIVCTMLSNSKEIAAMFGKRILDTVLQRLGKR
                     ITLQIEEGKSFRIMDDVYVTVLDCSDRSCVSRTMSVLTSDLEKPVAVSDTDVSPSVVS
                     GICQFPIDGERSESLLSNCLTAIFTAQRKGLKWEFYN"
     gene            complement(596..2740)
                     /locus_tag="PKL93_06835"
     CDS             complement(596..2740)
                     /locus_tag="PKL93_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71010.1"
                     /translation="MKEQMRKIVFLVIICIAFVAVFSGFGMLKRHNSDFELTESELVT
                     IEYGEIVSLPLSNFVKANGATVEFSLVGENGKIDGDFLVIDSSTLSLPEETIDIIVKS
                     EKSEAVLHITFSVKNLPPPPVLSIANQKVFEGDRLEIDLSELTFSEMNEISYEIVGGP
                     GLIEGDIYRYSSPVREAPVAHRVTIKAIDSLRQWHCETFSIIVIDKNHWPEEPHGPYP
                     GDGIEDFFNDLVLSWECSDPDGDILSYDLYFGTENLGLLAKGINKNTYELPPLEHGET
                     YSWQVVADDGNGGLVKGPVWSFSTEEIPVLTWKRIVGFEGRDSFNTVKSLSSGGYILC
                     GSSDPVSESESLKDSIPRVGWIVKIDNNGFMEWEKRFNLGWVELNDIIATSDDGFLVV
                     GKAIDKTAVGFDADSSLLVIKLDKFANEKWRFKSEGIFNEATSVIEVEDGYLILGTKT
                     ESKEDRSILLVKLGKSGSLEWQKSYGGSSFDRGVAFDVDGDGNVVIVAETTSKDGEAK
                     GNPGNRFQVNGIYIELSSVLTIKVNRDGEVIWSKVLGGKSEDSPSSVRINDEGEIIIT
                     GSTNSREETFQRDSSDYDGFAAKLSEEGDLLWARVYGGSGNDIIEDFFITQAGGIQAI
                     GFSESNRSINQPVDEAMKRSGITYSDFWVLQTDAEGELLWQKYLGGSKDDVGLSIAYV
                     CDGFVVAGYSASIDGDVGSNKGDFDAAVFYLR"
     gene            complement(2737..4980)
                     /locus_tag="PKL93_06840"
     CDS             complement(2737..4980)
                     /locus_tag="PKL93_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNQ71011.1"
                     /translation="MKKIALLASLLFSVVLFSIEYSPDSKAFSATSVAVSLDGEKLVA
                     GYIDNTIKVWSLLDGKLLNNLSFNSSWVTAVAVSPDGKKGIAGYDDGMIIVWDLATGR
                     KIKSLSSHTSTVNDLIVTKDSKTLFSCSSDATVKIWSLADLAFIRTLRGHSGSVFSIV
                     DWPEQSRLYSASEDGTLRIWDSSTGQQVKVIRAEIGALTAVSMDEGRSILILGSEAGQ
                     LKSYDVKTWSSLKTTRAHNAQITKILIDNEVVYTASSDRTIKLLSLPSFSLIKMITGH
                     TWDVTEIALSMDGHVLYSSSTDGKLKVWDIEKASAIGTLIGFGDGEYFSYNSQGNWVS
                     SSLAKERVTCENGSSPAEVGKELSSILLQLDKLPRIYTTSEQEVNLEKSTVSFTVSQP
                     VTRVTVNEEEVAIGEDGCVVFSPDGEGTYTLRAYDSSGSYDERILTVHFEETVMYVVK
                     EISSYQRGDKVLVDDHRNNEFLVNGEWVDETFLSISPPDLEPPIIIGERTQKVSIGKD
                     KYLTFSVSDNGTVTAAEVIAPDLTVSRRTLNSTSTEVVIEVNGTGVYTVNAYDSDGNK
                     TVATFYLEGEEEPFWVSKDYGSLRKGQQVKVAEEGESCYYVSDYGWVEKDCLSRSPVT
                     TGEPIIDGIPVQYASSAGEKILSFKVSDDVNVKEVVVSGKAYPVNEREREMHIIVNEY
                     GEHLVVAKDVEGNSSEAIFTLSAPDKNEKSNRSLFLLLIPILTLAVLIYIWVVRSSRK
                     KNRKVRL"
     gene            complement(5895..7160)
                     /locus_tag="PKL93_06845"
     CDS             complement(5895..7160)
                     /locus_tag="PKL93_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ71012.1"
                     /translation="MKIGVVGFGAASIGFISEILDEDHEIHVLERSKDIFSSSISGIR
                     SDGKIFVSETMGGDMEIPLAIQNEVVDFYLEKLNPDDRREVKRGVSFDRRSRFFDLFY
                     SKGFEPVNSTFWHIGTDKLSSVLTKIFEEFSTRNNLKFHFGSRVDDIDIEDEKIILRG
                     EGFEAVFDYVVVAVGRSGHSLIKKVTDRYPEIVASSNTVDIGVRFEIPDHVVEEINEE
                     MYEFKVRLKTKTGYTVRTFCNNPSGKVVLENYDDFVTVNGHSNSGGSSENTNFAILCT
                     TRFTEPFRDPIGYGSYISRLSNILAGGRKVILQTYEDFIQTKRTKRLGRVKPTLPEGD
                     YILGDINLVLPRRISVSITEFVEKLSEVIPGISYPDNLMYAVEVKFYSNKINNSRYKN
                     LKFIGDCSGHTRSITYATGHGRLTGYSLK"
     gene            complement(7204..8325)
                     /locus_tag="PKL93_06850"
     CDS             complement(7204..8325)
                     /locus_tag="PKL93_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNQ71013.1"
                     /translation="MNEIYKDVVWVDFDTLEAFMKDVFVGIGVPEEDAKICANVLITS
                     DKRGIDSHGIGRLKPIYVDRIRAGIQNPITEFEIVRESATTAVIDGHNGMGHVIAFKS
                     MKLAIEKAKRYGMGMVAVRNSTHYGIAGYYPIMAVENGMIGITGTNARPSIAPTFGVE
                     NMLGTNPLTFGIPTDEKFPFVLDCATSVAQRGKIEVYARAGKELPEGWVIGLDGRPRT
                     DTQKILIDLTKGEAALTPLGGIGEETAGYKGYGYATVVEILSAALQTGSYLKMLTGIA
                     DNNEKKPIPIGHFFIAIDIEPFTQLDDFKKLAGDILRELRKSKKAPGQERIYTAGEKE
                     YEAWQFRKNRGAPLNEVLRKQLVQLRDELRLHRYKFSWE"
     gene            8599..9180
                     /locus_tag="PKL93_06855"
     CDS             8599..9180
                     /locus_tag="PKL93_06855"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNQ71014.1"
                     /translation="MKDRIGPGIYLYPMPVVVIGTMNEERPNYTTIALCGVVEVQPPM
                     IQISLAKSRLSCKNIEDTGYFSVNVPSLDMLRGVDYMGIHSGKTTDKSELFRTFPGEK
                     GNVPMIEEAPLNMECELVSKFEFGGAHIAFVGEVVQTYCSESCLVDGLPNIKKISPVT
                     FSVYDNSYWSIGMHLGKAWNIGIKYTPPKEGTD"
     gene            complement(9207..10925)
                     /locus_tag="PKL93_06860"
     CDS             complement(9207..10925)
                     /locus_tag="PKL93_06860"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNQ71015.1"
                     /translation="MKVLGKCFLVLLLVSVSSSMACTTLIVTKGASADGSMIVAHSDD
                     NDLADQRIVYVPAQDHEPGSFRPVYCSAVAMGEFHQYNSFIYPRIVSDRAPAYDTPGY
                     PLSIPIGMIPQVDHTFAYFDGSYGIMNEHQLMFGECTDGAKIQIGPEPGKRIFYSSEL
                     SRVALERCKTAGEAVELIGYLIEKYGYYGTGETLPVADPNEAWVIEMAPSPEGTGGLW
                     VARRVPDGEVFVAANQFRIREIDTENPDFLYGETLHEVAEKFGWWDPKDGPLDWLRTV
                     SLGEYNHPYYSLRRVWRLFSSMAPSMNLSPWVEDGFTREYPFSLKPDNKVSASDVMNL
                     YRDHYEGTEFDLTKGVAAGPFGYPDRYYGPYDGQGDVGDPSRQLGGAWERPISVTYCG
                     YAFVCQARNWLPDPVGGIMWLGLDKPADTCFIPFFVGVSDLPESIQVCDTSEFNKDSA
                     WWAFNFVSNWAALKYSYMHEDIKLMQEKYEALSLSRLPEIEAHITKILLTKPEEAGRY
                     LARFCSRNSQEIVEAWWKLSEYLIVRYNDGFINEKGNMAQPTGYPKEWLDETGWADGP
                     TSYEKK"
     gene            11529..12593
                     /gene="corA"
                     /locus_tag="PKL93_06865"
     CDS             11529..12593
                     /gene="corA"
                     /locus_tag="PKL93_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNQ71016.1"
                     /translation="MDRKGIFRKRKPPLGSVPGTLSIDEESPFPKISLIEFDRDYLKE
                     EIIRNVENLASVISATDRVHWIDVQGLGDEATIRKLGEVFSLHPLALEDITNVPQVAK
                     VEEYDNCLFVCLPMMRMEEDSVVYEQMSLFLGETFVLTFQERYGDVLDPVRNRMRRNS
                     RIRSMDNDYTAYAILDTIVDNYFPVLQSIGSELEKLEEELVESISREKLKDLYLMKQK
                     LAQLRRLVWPTKDVIGRISREESEYFKDQTVLYLRDVYDHIIQSLDMLDISRELSSEL
                     MNLYLSSTSNRLNEIMKVLTIISTIFIPLSFIVGIYGMNFVYMPELSLKWGYPVVLIV
                     MGVVVFIMLLFIRRKGWFRK"
     gene            complement(12926..14599)
                     /locus_tag="PKL93_06870"
     CDS             complement(12926..14599)
                     /locus_tag="PKL93_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ71017.1"
                     /translation="MEDSAQFCLDLIDEAVVVVNKNGRVVFQNSVSQKHFGDLIGKLY
                     KAFFDCEDIFVDDEGVVWRVSSSSDKSDSNEIKIFRRTNSLSIGSSFTKREKTSASSF
                     FQGIDDHSTLHSMHSNTSFSILSEIDHFRVYKKAFEENTSGMAIFTVDMSREKIIPAA
                     KNLAFEINRLARLPVIEGIPLDRPMSKADHLFLKALIRVARTGRQEALFLSEEGFYEE
                     VLIKPLSDRDLISVHKEVSAEMNLRMELLSARYLAGTIDQAVISVDENGKIVFVNKAA
                     EILLGRERGGLIGKNINYFLENQSDSRSEKPSAGQEIGERHLFSINKSNGQSVLVEAA
                     VKRIEDSSLAGKVTATVYCLSGLKFEDVIQESAQKTIRGLIETLSTVVEVRDPFTAGH
                     QRRVSKLATAIATEMGLGPEQIDTVREGALLHDIGKISVPTEILSKPGRLTENEWFLI
                     QSHSEAGFKMLERIDLPWPISSIVRKHHERLDGSGYPHGLKGKDIPFETRIVTVADVF
                     EAIVSHRPYRPAHETSTAITELTEGAGRLYDGDVVDCCLSLVGKGFSLD"
     gene            complement(14808..15755)
                     /locus_tag="PKL93_06875"
     CDS             complement(14808..15755)
                     /locus_tag="PKL93_06875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ71018.1"
                     /translation="MRRSGHTEIDASKLKALILDLDGTLLDNSKRIPASALNAIERLQ
                     EKGIKLAIFTGRDYLMAREYLDVLNIEGPHALQNGAFLAKGKGEIFLMRCIERKITEY
                     SVSLASILNCHLLLRTDSSEIPDWYHVGDFALSPYLPFLESNINRIVRLDDLTEVVKS
                     ANLLQLDITGDLESLKEILGRLTSKYTGDFNFSFISASDDAGFLDVYWSQMEDTAVRL
                     EIASKVDSWGLLSLFNASVSKGLSLDDFLNYHGLASQQVAFIGDHYADIPLLKRVGLS
                     VAVENALPEVKAVCDYVTQSNNDDGVALAIEEIFFSGKR"
     gene            complement(15752..16393)
                     /locus_tag="PKL93_06880"
     CDS             complement(15752..16393)
                     /locus_tag="PKL93_06880"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNQ71019.1"
                     /translation="MINVRSYHIVAALFQLGNSSRMKNIHILSMKYNRILKLKEAEMN
                     TGFVQIYTGNGKGKTTAAIGLAVRAALAGERVFIGQFIKGMRYSELSLPNYIENIVIE
                     QFGRECFIIRDPETKDLEVARKGLARSREVISSREFGVVILDEIFIAFHYRLVSSMDI
                     RRLMLSKPKEVELVLTGRNAPAEIIREADLVTEMREIKHYYSKGVLAREGIEY"
     gene            16507..16746
                     /locus_tag="PKL93_06885"
     CDS             16507..16746
                     /locus_tag="PKL93_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNQ71020.1"
                     /translation="MAKYEVTKSVDVRGEVCPVPDVETKRALKKMKSGEILEVWIDYA
                     MSKERIPEAVKSMGHEVLEIEEVGNSEWKIYIKVK"
     gene            16766..17755
                     /locus_tag="PKL93_06890"
     CDS             16766..17755
                     /locus_tag="PKL93_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNQ71021.1"
                     /translation="MAWTGLLVGLVFGIILQRGRVCFNSAFRDVLLFKDNYLWKLGFL
                     AVGLQMITVLFVAQMGWIKIAPPTLNLFGNIVGAYVFGLGMVLAGGCASGVTYRSGEG
                     MTTAMIAAVFYGIGAMAMRGGVFSPIRTWASQFNVSVDANSSVYLDKVGPTLATVLNI
                     NPWIPAVILAAVLLWYTLGTKTTERKTKFNWKGAAVSLAILSPIAWITSEAAGRNYGF
                     GITGGWVSIFDSYISNQPLRWDGFEIIGIILGALIASLLAKEFKLRMPKNPKTYLVVM
                     IGGTMMGAGASLAGGCNIGHFLAGLPTLAISSIIASVFLILGNWTMAYILYRK"
     gene            17766..18122
                     /locus_tag="PKL93_06895"
     CDS             17766..18122
                     /locus_tag="PKL93_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNQ71022.1"
                     /translation="MRITIQVFAPPYSYEDLDSAIKIAEAGLNKGHEVTIFLFADSIL
                     SINSKVKPIRVDRDIPRKLESMIKEKGLKVEICGICMDYRGVTKDMIIEGSNPSGLPE
                     LASLIFNSDRFVNLMA"
     gene            18131..18487
                     /locus_tag="PKL93_06900"
     CDS             18131..18487
                     /locus_tag="PKL93_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular sulfur oxidation protein"
                     /protein_id="HNQ71023.1"
                     /translation="MDKKILFVVYQAPAGSIWVNEAFRTAFGMYGEDIEPSVLLMEEA
                     TVALSKKTKPECLGLLSISMCFRFIKRYETAVYGVREHVERFKVKEIEESFNAKLIGE
                     ADLGDFFHDFDNVVFM"
     gene            18498..18761
                     /gene="tusB"
                     /locus_tag="PKL93_06905"
     CDS             18498..18761
                     /gene="tusB"
                     /locus_tag="PKL93_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HNQ71024.1"
                     /translation="MHLIVVKNGPNNPAERVKIRASKEGDSVVLIQDGIFWALNDIET
                     EAKCFAIKDDVEARGYTADDVTVPLISYDGFIDIIEKCDKSIG"
     gene            18833..19411
                     /locus_tag="PKL93_06910"
     CDS             18833..19411
                     /locus_tag="PKL93_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNQ71025.1"
                     /translation="MPLKLPDLFRTLSNQTRLEILTMLMDNYLTATEIATLLQIDLST
                     VYRHLKQMKKLGILTSTHLHGVERFDFSSPHIFRMLDEAITFMGELKGFSPIVCSEGI
                     CSYYLGGELDEIEPDQLLDMRGESCPVPDIQARKTLRKMNPGEILLVIVDYPLSGERI
                     PASVQKEGHEFLKKVADNYGDIKIYIRRRENG"
     gene            19404..20765
                     /gene="lpdA"
                     /locus_tag="PKL93_06915"
     CDS             19404..20765
                     /gene="lpdA"
                     /locus_tag="PKL93_06915"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNQ71026.1"
                     /translation="MVDAVVIGGGPGGYVCAIRIAQLGKSVALVEKENLGGTCTNWGC
                     IPTKAMLTSAHLYTEINEKSKRLGIDASGLGYDLKRIMSHMNRTITMSRKGIEHLLKK
                     NGVDFYNDVAEIKDAGHVLLKNHGKTLETRNIVIAAGSEPSIFKPFSEIEGIWTSNDV
                     FQMEEMPESLVIVGGGVIGVEFATFFSSFGVKTTIIELADHILPYEDRDVADDIRKSL
                     TRQGVEIVERTKVTEVEKDECIFVLNAEGEQELSVQAEKVLVAVGRRPYITEDVRKLG
                     LEIERGVVTNSRMQTNIKGVYAIGDIRAGMMLAHVASYEGIVAAHNIAGEVMEMNYSA
                     VPSIIFSNPEVGSTGIKEDDVEDMERVIIAKFPLSANGRARTVLENTGFVKVIADKEN
                     GKVLGMSIVSPSATELIMEGVIAVKNGLTVEELENSIHPHPTLSETVLGALEGVNGMS
                     IHI"
     gene            complement(20970..22640)
                     /locus_tag="PKL93_06920"
     CDS             complement(20970..22640)
                     /locus_tag="PKL93_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71027.1"
                     /translation="MKNLSRSFLLFVASVIVVFSLSSCIVVDNPVLVRVPFEELKGGA
                     YYGGGLLFSPSDMSISHLGEGTLIAFQDLRMEWAPEEGSSIAERNYGYLYIKSIDGTG
                     IEYEYLIYDSEGAIVKHVDSVFQPVSAGSQDLSSSSDADFFSGVSYHSAAGMQDPYIS
                     SGYLLTFRHEIPDAESSGPVSFRKVLFRVQQPPMVSSTRFPSGVIAVSNSIPRSLVIN
                     SSLLVQEPSAIENQIVFSKTSSLPNFSNGDYIFDTMKDSLRRVVSVNDSDPSKVTLET
                     EEAFVEEALGTLILNIEGDVSEIIEKFGTESDRSFAESVTTNLITKEWEVSIVDDDEM
                     KAAIMNKLVLDANISLDFHLSWNKFSSNGTLRFPAKVSSILEIVGKEGFETENTKKVA
                     EPEVSISVCGIPVKVSIPIELFYGMETQIESIDLQFGPEIEVYPKFHYEVEAKIDFHG
                     PHGKSYTRGSIDHNSTFVDKIDFDGSPEIEGKFGFKFFPGVTIACVLRPEMEIPMGFS
                     AKVNQSKAELDFETEGHIMIKLDLLFYKHTFAKTKVFSQTRNIYSKEF"
     gene            complement(22873..23256)
                     /locus_tag="PKL93_06925"
     CDS             complement(22873..23256)
                     /locus_tag="PKL93_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71028.1"
                     /translation="MKKTDLDLERLNSTSWKWLNFEEAELSGGENSRQPQAMYFFTDE
                     KNRSFCRRYNREVNFKRDVEDIGLCDKCGMRFRTYRAAQICVFAPAEAYYTIDTRDEA
                     KEIEEFPDKDFAEKGVSDFESKSWQ"
     gene            23328..24974
                     /locus_tag="PKL93_06930"
     CDS             23328..24974
                     /locus_tag="PKL93_06930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ71029.1"
                     /translation="MILKKFLLILLAAFFSLTLIGAEYAVRITKGGEAVSKEFWITRE
                     EIEQAFNATVANAASQGIILDPYFDSYFTPSELGLKTMIIPYIVDEKLIDYFAWESNL
                     IPSEEEIDAETDSMMEMYTSSPDMVEQIEAIYGSMDAFRSEIRNYVSDALKAELVQES
                     VAPLNDDALAAYFEEFKTEIKNQFETIRARHILVTEEATATELMDRINSGEITFAEAA
                     LQFSIDSSTAANGGELGSIVRGQTVPEFEEAILAAPIGELYGPVQSEFGYHLIIVEER
                     NEINSLEDVVNSASYNDFVSGYQNDTYNRWIESYIEENKFDYEILDNELLFYNEYAKA
                     KATEETANEFFVEIASGIFGDGTSSEASLMEYAAFIELSEMLGFTDSPDYETAIRNLF
                     EAGEKRGMIVQKMYDLDSENPEVAAAYYNALLEELENTFMNQDLLQQQLSSYGQSFVD
                     YVFNTIGEIESGLTSVLEKEISDGLRADVLYILIRNNSLSLELDYSPDWRKEKLNQRL
                     GYLEALIQVEPSEAAQTEIESIISELEQIEAEAETPAATE"
     gene            complement(25091..26386)
                     /locus_tag="PKL93_06935"
     CDS             complement(25091..26386)
                     /locus_tag="PKL93_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ71030.1"
                     /translation="MKGLGRNFWLFTLGRLVSLIGSGVQALAIPLYILDLTGSGVMMG
                     TFSVVTMLPRILFGPIAGVLGDRFNRKYIMIYMDFARGAAIILMAYLSDVGVLNLALL
                     FVFQLVISTFDISFDPATSAMLPDIIEEHKLLKANSILGAVNSVSYIVGPALGGVLYG
                     LFGIEIVFIINGTSFILSAISEIFIVYQQTTKKTRVSVKSVFVDIVEGIGYLKKIDGL
                     MMVLMFAMVSNFLSTPIFTVVFPFFARTLVGFSSEQYGFLQSGWVLGVLFGNILIGTI
                     LVNKKQDKLFAEGLVTETVLNFLLAALFFPYFTSLFGGPSWLFFAALGIPIVVMGVFN
                     AFVNTPLQTLFHRVLPTSHRSRVFSVISILAQIATPLGSAVFGFAVDRLPVHYLVLIS
                     CIANAILTIVFLAKGMSRLFEVRPGKHGGDSYGNAEEIA"
     gene            26699..27721
                     /locus_tag="PKL93_06940"
     CDS             26699..27721
                     /locus_tag="PKL93_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNQ71031.1"
                     /translation="MSRVVTFGEIMMRLASPLGERFEQTKNFEVVYGGAEANVAVAIA
                     KYGGKSRFVSKVSADQFGDAAIGNIGVHGVDTSYVLKDIGRLGKYFYEYGYSQRPSKV
                     IYDRDNSVFANSKPGDFDWEEVFSDATWFHFTGITPALSDGLIETCEVALREAKKRGI
                     TVSCDLNYRSRLWSKEKARKVMTSLMNNIDVLFANEEDSESVFGIKAEGTNVSDGRLS
                     VEGYRDVATKLRDTFDLKAVAISLRESINANFNKWSAALLTGGEFIVSTKYDINIIDR
                     VGGGDAFAAGLIFGLDNSWEPLKSLEFGVAASCLKHTIKGDFNIVSRDEVEKLLGGNA
                     SGRVVR"
     gene            27912..28961
                     /gene="thrC"
                     /locus_tag="PKL93_06945"
                     /pseudo
     CDS             27912..28961
                     /gene="thrC"
                     /locus_tag="PKL93_06945"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
BASE COUNT         8267 a         6257 c         6215 g         8293 t
ORIGIN      
        1 accatttacg atagtgattt acagtgtcga gatttcggaa aggctttgtt acttcatttc
       61 cttgagggat aacagtgaat ctactgttct tagaaacatt ttaagagata ttatttacat
      121 cgatccatta tcgggattcg gaaacgaaag atctttaaat agatttctct caaagaaaat
      181 ctcctcctcg aaggaaaatg gagcttgttt ttcgattgtt tgcactatgt tgtccaattc
      241 caaagagata gctgcaatgt ttggaaagag gatactagat accgtattgc aaaggcttgg
      301 aaagagaatc acacttcaga tagaagaagg caagtcgttt agaatcatgg atgatgtgta
      361 tgtaactgta ctggattgca gcgatagaag ttgcgtttct agaactatgt cggttctcac
      421 atcagatttg gagaaaccag ttgccgtttc agatactgat gtatcacctt ctgtcgtgag
      481 tggaatttgc cagttcccca ttgatggaga aagatctgaa tcacttctca gcaattgcct
      541 cacagcgatt tttacagctc agagaaaagg cttgaagtgg gaattttaca actgattatc
      601 taagataaaa aaccgctgca tcaaaatcac ccttgttaga gccaacgtct ccgtcaattg
      661 aagctgaata gccagcaaca acgaatccat cacaaacata agcgattgat agtcctacat
      721 cgtctttact gcctccaaga tacttttgcc ataagagctc tccttcagca tcagtttgca
      781 gcacccagaa atcactgtaa gttataccag atctcttcat ggcctcgtct acgggctggt
      841 tgattgatcg atttgattca gaaaagccaa tcgcttgaat gccacccgct tgcgtgatga
      901 agaagtcttc gattatatcg ttaccagatc ccccatagac tcttgcccac aataaatcac
      961 cttcttcaga tagttttgct gcaaatccgt cgtagtcgga agaatctcgc tggaaagttt
     1021 cttccctgga atttgtggag cctgtgataa ttatttctcc ttcgtcattt attctgacag
     1081 aagaaggcga atcctcgctc tttcctccaa gcactttcga ccatataact tctccgtctc
     1141 tattgacctt gattgtaaga acagaagata attcaatgta gattccattt acctgaaagc
     1201 ggttgccggg atttcccttt gcttcaccgt ctttcgaagt tgtctcggcg acaatcacaa
     1261 cgttgccatc accgtcgaca tcaaatgcaa ctcctcgatc aaaagagctt cctccatagg
     1321 atttttgcca ttctagggaa cctgatttgc ctagttttac taacaggatc gaacgatctt
     1381 cttttgactc cgtctttgtt cctagaatca agtatccatc ctctacctca attactgagg
     1441 tcgcctcatt gaagataccc tcactcttga atctccactt ttcattcgca aacttatcaa
     1501 gctttattac aagcagtgaa gaatccgcgt cgaatccaac agcggtttta tcgattgctt
     1561 tacccactac caggaatcca tcatccgagg tggctatgat gtcattaagc tctacccaac
     1621 caagattaaa tctcttctcc cactccatga aaccgttatt gtcgatcttt acgatccatc
     1681 caacccttgg aatcgagtct ttcaatgatt cggattccga aaccggatct gaggaaccgc
     1741 aaaggatgta tccaccactg gacagagact ttactgtgtt aaaggagtct cttccttcaa
     1801 aaccgacaat tctcttccat gtcaaaacag gtatttcttc tgtcgaaaaa gaccagacgg
     1861 gaccctttac caatcccccg ttcccatcat ctgcaacaac ctgccagctg taagtttctc
     1921 cgtgttctag aggtggcagc tcgtacgtat tcttgttaat gccttttgca agtaaaccaa
     1981 ggttctcggt gccaaaatag aggtcataag acaaaatatc accgtcgggg tcactacact
     2041 cccaagatag aactaggtca ttaaagaagt cttcaatccc atcaccggga taaggaccat
     2101 ggggctcttc gggccagtga tttttgtcga taacaattat gctgaaagtt tcacagtgcc
     2161 actgccgcaa actgtctata gcttttatag ttaccctgtg ggcgacaggc gcctcgcgta
     2221 ccggagaact gtaacggtag atatctcctt caattaaccc cggcccaccg acaatttcgt
     2281 aacttatctc gttcatttca gaaaaggtga gttctgagag atcgatctct agtctgtcgc
     2341 cttcaaagac tttctggttt gcaatactta gaaccggggg tggtggcaag ttctttactg
     2401 agaatgtaat gtgaagcaca gcttccgact tttcagactt aacaatgata tcgattgttt
     2461 cttcaggtaa gctaagcgta ctcgaatcaa ttacgaggaa gtcaccatct atctttccgt
     2521 tttccccaac caatgaaaac tcaacggttg ccccatttgc cttgacaaaa ttagacagtg
     2581 gtagtgaaac aatttctcca tattctattg ttactaattc ggactcagtt aactcgaaat
     2641 ctgaattgtg tcttttcaac attccaaatc cagagaaaac cgctacaaac gcaatacata
     2701 ttataacgag gaaaacgatt tttctcattt gttctttcaa agccttacct ttcggttctt
     2761 tttcctcgac gatctcacaa cccaaatgta aatcagtact gcaagtgtca aaatcgggat
     2821 tagcaacaaa aacagactcc tattgctctt ttcattttta tcaggggccg acagagtaaa
     2881 aatcgcttcc gaggaatttc cctcgacatc ttttgcaaca acaaggtgtt caccatattc
     2941 gttcacaata atgtgcattt ccctttccct ttcatttact ggataggcct tcccggaaac
     3001 cacaacctct tttacgttta cgtcgtcact gactttgaag cttaaaatct tttcgcctgc
     3061 agaagacgca tactgaacag gaattccatc aattatcggc tcccccgttg tgacagggct
     3121 tcttgagaga cagtcctttt caacccagcc ataatctgaa acgtagtagc agctctcgcc
     3181 ttcttcagca accttaactt gctgaccttt cctcaggctg ccataatcct tggagaccca
     3241 gaagggttcc tcttctccct ctagataaaa ggtagcaact gtcttattcc catcggaatc
     3301 gtaggcgttg acagtgtaaa caccagttcc gttaacttca ataacaactt ccgttgatgt
     3361 tgaattgaga gtgcgacgcg aaacggtaag atcgggcgct attacttctg cagctgtcac
     3421 agttccgtta tctgaaacag aaaatgtcaa gtacttatct ttcccaatgg agactttttg
     3481 agttctttcg ccgattatga taggaggttc aagatctgga ggtgatatcg ataaaaaggt
     3541 ttcatcaacc cattccccat taactaggaa ttcgttgttt cggtgatcgt ccactaatac
     3601 tttatctcct ctttgatatg agctgatttc cttcaccaca tacatgaccg tctcttcgaa
     3661 gtgaacagtg aggattcttt cgtcgtagga accggagctg tcataagctc taagtgtgta
     3721 agttccttca ccatcagggg agaaaactac gcagccatct tctccaatcg ctacctcttc
     3781 ttcattcaca gttactcttg ttacgggctg ggaaactgtg aaacttacgg tgctcttttc
     3841 taaatttact tcttgttcac ttgtagtgta gattcttggc aacttatcaa gttgaagaag
     3901 aatggaactc aattccttac cgacctcagc agggctagat ccattttcac aggttactct
     3961 ctcctttgcc aatgatgaag agacccagtt tccctgggaa ttatatgaaa aatactcccc
     4021 atcaccaaaa ccaatcaatg ttcctattgc tgaagccttt tcaatgtccc ataccttcaa
     4081 tttgccatct gtcgaggaag aatagaggac atgaccatcc attgaaagag ctatctcagt
     4141 aacgtcccag gtgtgcccag ttatcatttt tataaggcta aacgaaggca gagagagaag
     4201 ttttattgtc ctgtcactgg acgcagtata aacaacttca ttgtcaatca agatttttgt
     4261 tatctgtgca ttgtgagctc ttgtggtctt aagtgacgac caggtcttga catcgtagga
     4321 tttcagttgt cccgcctcag atccaagaat taggatcgac cttccttcat ccatggagac
     4381 tgctgtgagg gcccctattt ccgctctgat tacctttacc tgctgacccg tggaagaatc
     4441 ccagattcta agggttccat cttctgaagc agagtacagt cgactctgtt caggccaatc
     4501 cacaattgaa aagaccgatc cggaatggcc tctaagcgtt ctaataaaag ccaggtcagc
     4561 aagcgaccag atcttcacgg tagcatctga gctacaggaa aacaacgtct tgctgtcttt
     4621 cgtcacaatt agatcgttta ccgtgcttgt atgactgcta agcgatttga tttttcttcc
     4681 cgtggcaaga tcccacacga taatcatccc atcgtcgtac ccggcgatac ccttcttacc
     4741 atcgggcgaa acagcgacgg cggttaccca tgaactattg aaagaaaggt tgttcagtag
     4801 tttaccatcc aaaaggctcc agactttgat tgtgttatca atataacctg cgacaagttt
     4861 ctctccgtct aaacttactg ccactgaagt agcgctgaat gccttgctgt ccggtgaata
     4921 ctctatactg aacagtacca ctgagaaaag caaactagcc agtagagcta tttttttcaa
     4981 ttttatcacc ctgattgaag ttttcaatca attctaccat tcaagaatac taaagaactg
     5041 aacaacaatt ctcatagaaa tttctgtatc tccaaccgat aaccatttgg atcctttgta
     5101 aagaaatgat agatcttgaa ttcctggctt acggttggtt cggaacacgt cacgccagca
     5161 tctttcaatt tgcggtagaa ttcatcaacc tcattggtca acaaggtaat gatagagtta
     5221 tttctttgag agtcaagatt gagatgattg cagaagccta tcattccacc gccttgagtt
     5281 aggaatattt gacagctatt ctgatcttta tatagtttaa ggccaagaag gcttacataa
     5341 aacgagtttg ttaaacctag atcttcagtt ccgaagaaga cgatagaatt ctcaatattc
     5401 actaactgtt cctcccttca aagtaaaggt ccgatcagcg atcggaccct tccggttgat
     5461 ctttcatacg ttactttggt gccttgagga gaagtcgcac ttaaaagggt gttggtgtgc
     5521 tttgcgccgg tcctacaact ctttcttagt cgatgtgtct ttcatctcgg cttttctgaa
     5581 ttgagacctt tgaacggtgt ctaaagttca tgagaattgt gtagtttgag tttagtcaaa
     5641 tttaaatgta agttacacaa gcttattgat gttcatgaaa gacccctaga gcaactttaa
     5701 aaagaacatt tcaaaagcac tcttagcaaa agcgacctcc aaaggcttaa ctaatggaga
     5761 agctctttgg ttaaacagcg aagcaaatcc tcttcacttc gatggaaatt tttcttagta
     5821 acatcaaaac tctctcgaag gcaatctcct tggtaacgac agtttcgcat cctaaacccg
     5881 gtcatttttg gatttcattt cagagaataa ccggtaagcc ttccgtgtcc agttgcgtag
     5941 gtaattgatc tcgtatggcc gctgcaatcg ccaatgaatt tcagattctt atagcgactg
     6001 ttgttgatct tgtttgagta aaatttgact tccacggcat acatgagatt gtctggataa
     6061 gagattcccg ggattacttc acttagtttc tcaacaaatt ctgtaatgga aacggatatt
     6121 ctccttggca gcacaagatt aatatctcca agaatataat ctccttcagg tagcgtgggc
     6181 ttaactctcc ctaacctctt agttctcttg gtttgtatga agtcctcgta agtctgaagg
     6241 atcactttcc tgccaccagc cagaatatta cttagcctgc taatatatga gccatatcct
     6301 attggatcac gaaagggctc agtaaatcgc gtagtacaaa gtatcgcaaa attggtgttt
     6361 tcagaactcc cacccgagtt cgagtgacca ttcactgtca caaaatcatc gtagttctct
     6421 agaactacct ttcctgaagg gttattgcag aaggttctta ctgtatatcc tgtcttggtt
     6481 ttgagtctca ctttaaactc gtacatctct tcgttgattt cttccaccac atgatctggg
     6541 atttcaaatc taactccaat atcgactgtg ttgctagaag ccacgatttc aggatagcgg
     6601 tctgtcactt tctttattag tgaatggccg ctccttccaa cagctaccac gacatagtca
     6661 aatacagcct cgaatccctc gccgcgaagg attattttct cgtcttcgat gtctatgtca
     6721 tccactctag aaccaaagtg aaatttaaga ttatttctag tcgagaattc ctcaaatatc
     6781 tttgtaagca ccgaacttag tttatctgta cctatatgcc agaaggtaga attaacaggc
     6841 tcgaagcctt ttgaataaaa gagatcgaag aaacgcgatc ttctatcaaa agagacacct
     6901 cttttcacct cgcgcctatc atctggattg agtttttcca ggtagaagtc aacgacttca
     6961 ttctggatcg cgagaggaat ctccatgtct cctcccattg tttccgacac gaagatcttt
     7021 ccatcggacc gtattccgct tatactggaa ctgaaaatat cttttgaacg ctccagaaca
     7081 tgaatttcat gatcctcatc taaaatttca cttatgaaac ctatcgatgc tgctccaaat
     7141 cccacaactc cgattttcat tgttttgctc ctaaaagaga aagtttgtct tcagggatag
     7201 atattattcc cagctgaact tgtacctatg aagccgcagt tcgtcccgta gttgcacaag
     7261 ttgctttctt aaaacctcgt ttaaaggggc acctctgttt ttcctaaatt gccaggcttc
     7321 atattccttt tcacctgcgg tgtagattct ttcttgaccc ggtgcctttt tggatttcct
     7381 gagttctctg agaatgtctc ccgcaagctt cttaaagtca tctagttgtg tgaacggttc
     7441 tatgtctatc gcgatgaaga aatggcctat tggaatcggc tttttttcgt tgttatcggc
     7501 aattcctgtt aacatcttca gatagcttcc cgtttgcagc gcggccgaca gaatctcaac
     7561 aacagttgcg tagccgtatc ccttataccc ggctgtctcc tcgcctatgc cgcccagggg
     7621 agttagagcg gcttcaccct ttgtcaaatc aatcagtatt ttttgagtgt cggtcctggg
     7681 tctcccatct agaccgatga cccatccctc tggtaactct tttcccgctc gagcgtacac
     7741 ctcgatcttt cctctttgtg ccacagaagt agcgcagtcg agaacaaaag gaaacttctc
     7801 atctgtaggg attccaaaag tcagggggtt cgttcccaac atattttcca caccaaatgt
     7861 cggggcaatt gacggacgag cattagtgcc tgtaatgcct atcattccgt tttcaactgc
     7921 cataattggg tagtaacccg ctattccata atgtgttgaa tttcttacgg caaccattcc
     7981 cattccatat ctctttgctt tttcaatagc aagtttcatt gatttgaacg ctatcacatg
     8041 acccattcca ttgtggccgt caattactgc tgtcgtagca ctttctctga cgatttcgaa
     8101 ctcagtgatg ggattttgaa tgccagcgcg gattcgatcc acatagatag gtttcaatct
     8161 gccaatacca tgtgaatcaa ttccccgttt atctgaggtg atgagaacat ttgcgcagat
     8221 tttcgcgtct tcttctggaa ccccgattcc aacaaataca tccttcatga aagcttccag
     8281 agtatcaaaa tcgacccaaa ctacatcttt gtagatttcg ttcacaagat tacctccctt
     8341 ttctcctcct tctgacatta tacaatctgt gaaagacatc ttcttttttt ttgttttcct
     8401 gaagcatcga atctccgtaa atcaagagaa gcagaatccg ttgagatatt tacatgaaac
     8461 gcgatcgctt cttataaatc gtcaaacgag tgtacagctt gcttttcaaa ggcagaatcg
     8521 agtagaatta attgatgtag gatagatgaa gaagatattt tatattatca atgatgcagg
     8581 aaagattggt gaagaagttt gaaggacaga attggaccag gaatatacct ctaccctatg
     8641 cctgtggtgg ttataggaac gatgaatgaa gagaggccta attacaccac gatcgcgctt
     8701 tgtggagttg tggaggtgca gccgcccatg attcagatct ctctggcaaa gagcagactg
     8761 agttgcaaga atatagagga taccgggtat ttcagtgtga atgttccttc ccttgacatg
     8821 ttaagagggg tagactacat gggaattcat tctgggaaga ccactgacaa atcagaactc
     8881 tttagaacct ttccaggtga aaagggaaat gtgcctatga ttgaagaggc acctttgaat
     8941 atggaatgtg agctggttag taaatttgag ttcggtggag cacacattgc atttgttgga
     9001 gaggttgttc aaacatattg ttctgaatcg tgtcttgttg atggacttcc aaatatcaag
     9061 aagataagtc cggtaacctt ctccgtttat gacaattctt attggtccat tggtatgcac
     9121 cttgggaagg catggaatat tggaataaaa tacacccctc caaaagaagg cacggattag
     9181 atccttcgaa ggaggggtct tgagctctac ttcttttcgt atgatgttgg cccatcggcc
     9241 cagccagttt cgtccaacca ttctttgggg taaccggttg gttgagccat gttccctttt
     9301 tcatttatga acccatcatt atatctcaca atgagatact cggacagttt ccaccatgct
     9361 tcaactattt cctgggagtt tctagagcag aatctcgcta gatatctgcc cgcttcttcg
     9421 ggctttgtga gaaggatttt tgttatatgg gcttcgattt ccggaagacg actgagtgat
     9481 agtgcttcat acttctcttg cataagttta atatcctcgt gcatatagct atatttcaat
     9541 gccgcccaat tagaaacgaa gttgaatgcc caccaggcgc tgtctttatt gaactccgaa
     9601 gtgtcgcaga cttgaatcga ctcaggaaga tcgcttactc ctacgaagaa aggaatgaaa
     9661 caagtatcgg ccggtttgtc gagcccgagc cacatgattc caccaaccgg atctggaagc
     9721 caatttctcg cttgacagac aaaagcataa ccacagtacg taaccgaaat tggtctttcc
     9781 catgcacctc caagttggcg ggaaggatcc cccacgtcgc cctgtccgtc ataaggaccg
     9841 taatatctat ctggataacc gaacggcccg gccgcgacgc cctttgtgag atcaaactct
     9901 gttccttcgt agtgatccct gtatagattc atcacatcac tggctgagac cttgttgtca
     9961 ggcttcagcg aaaatggata ctctcgagtg aacccgtctt ctacccatgg agacagattc
    10021 atgcttggtg ccatggacga aaaaaggcgc cacactcttc taagcgagta gtatggatgg
    10081 ttgtactcac ccaggctaac ggttctcaac cagtctagag ggccgtcttt cggatcccac
    10141 catccgaatt tctcagcgac ttcatgaagg gtttctccat aaagaaaatc gggattttca
    10201 gtatcgattt cccttattct aaactgattt gccgcaacga agacctctcc atcgggtact
    10261 ctccttgcga cccaaagacc tccggttccc tcgggagagg gagccatttc gataacccaa
    10321 gcttcattag ggtctgcaac gggcagagtc tctcccgttc cgtagtatcc atatttctca
    10381 atcaagtagc caatcaactc aacagcttca ccagctgtct tgcatctctc aagggctact
    10441 cgagaaagct cggatgagta gaaaattctt ttgccaggtt ctggaccgat ctgaatctta
    10501 gcaccgtcgg tacattcacc aaacatcaac tgatgttcat tcataatccc ataagaccca
    10561 tcaaaataag cgaaagtatg atctacttga gggatcattc cgatgggaat tgaaagggga
    10621 taccctggag tatcatatgc cggagctcta tcagacacga ttctcggata aatgaagctg
    10681 ttgtactggt gaaattcacc catcgcgact gcggagcagt acacaggtct gaatgagcca
    10741 ggttcatgat cctgagctgg tacatatact attctctgat cggccagatc gttatcgtct
    10801 gaatgagcga caatcatcga tccatctgca ctagcacctt tcgttacaat caaagtcgta
    10861 caggccattg aactactcac agaaactaaa agaagaacaa gaaaacattt tcccagaact
    10921 ttcacggcac ctcacctctt ctctcttaag aaaaatggag actattccag atagaataat
    10981 cttcaggaat aatctatgtg agtctctaat ctcaagctca tcattggtgg agaagacgaa
    11041 aattcggctg atgaatcaaa gcatcttcga accgatcgac tacatttctg tgtcggctgt
    11101 gagtctttcc aaactgcttc ttcctaatgg gagaaggggc aaaccaaaca taatcgtttt
    11161 ttgaagatga ttccgaacca ggtttttgaa atctacacaa caatactaaa tcaagaccat
    11221 aaatcgaagg cattctcaaa tctcggatgt ttcaaatctg ttttgattcg actgaacaga
    11281 gaattgcgaa atcgatggaa caaaagggag tgaatcaaag atctctgagc gattctccgt
    11341 gctcacgact gattcttgtg actttgatac taatatactc ccgcaggttt gaaatccaaa
    11401 ggaaaaattg attctttttc tttgctccgc actctcttag aaagtgttac taaaattcga
    11461 ccggtgatag tatatttgag ctatattgaa agtcaaatca ctttgatttt tgtttaggga
    11521 ggcacttaat ggatcgaaag ggaattttca ggaagagaaa accgccgctc ggttccgttc
    11581 ctgggactct tagtatagat gaagaatcac catttccaaa gatcagtctc attgaatttg
    11641 acagagatta cttgaaagaa gaaataattc gaaacgtaga gaatctggcg tcggtcatct
    11701 ccgctaccga cagagtccac tggattgatg tgcagggatt aggagacgaa gcgacaatcc
    11761 gaaagctggg tgaagtcttt tctctccacc cgctggctct cgaagatatc acaaatgttc
    11821 cgcaggttgc aaaggttgag gagtatgaca attgcttgtt tgtttgtctt cccatgatgc
    11881 gaatggaaga agattcagtt gtttatgaac agatgagtct cttcttgggc gagacttttg
    11941 tacttacgtt ccaggagagg tacggggacg ttctcgaccc cgttagaaac agaatgcgga
    12001 ggaactcaag aataagaagt atggacaacg attacactgc atatgcaata ttggacacaa
    12061 tcgtggataa ctactttcca gtcttgcaaa gtatcggcag tgaacttgaa aaacttgaag
    12121 aagagttagt tgagagcatc tccagagaaa agcttaagga tctatatcta atgaagcaaa
    12181 aattagccca attgagaagg cttgtgtggc ctaccaaaga tgttataggg cgaatctcca
    12241 gagaagaaag cgagtacttc aaagatcaga ctgttcttta tcttagagat gtttacgatc
    12301 acataattca gtcactggat atgcttgata tcagcagaga gctctcgtca gagctcatga
    12361 acttatacct ctcttccaca agtaacagat tgaatgaaat aatgaaagtg ctgacaatta
    12421 tctccactat cttcatacca ctatccttta tcgtcggaat ctatgggatg aatttcgttt
    12481 acatgccaga gctttcctta aagtggggat atcccgttgt tcttattgtc atgggtgtag
    12541 tggttttcat aatgctcttg ttcatacgca gaaagggatg gttcaggaaa tgattcccca
    12601 cttgtagtta tctttgtggg caaactattg gcaggtcttc cggaaaggga gaaaagttcg
    12661 cttatatgat atggagagaa cgctttctat cacgagaggt tttgttggat catcttggga
    12721 cggatttcaa agagagatat acccttcttg cgcaagggct agcgcttatt tattaatcta
    12781 agatattttc aggtagcttg agtataattt agatcttaac aaagaacctg tatcatttcc
    12841 gattaagcaa aaaactagat acttaatttt gcctttgagc cattggttca caatgttatg
    12901 cgaagatctc tggcacggcg ttcattcagt caagagaaaa gccctttcca acgagagaaa
    12961 ggcaacaatc tacaacatcg ccatcgtaca gccttccggc tccctctgta agttcagtaa
    13021 ttgctgttga tgtttcgtga gcaggtctgt aaggtctgtg agaaacgatg gcttcaaata
    13081 catcggcaac ggtgacaatc ctggtttcaa agggaatgtc tttgcctttc aagccatggg
    13141 gataacccga gccgtctaat ctctcatggt gcttcctgac aatgcttgat attggccagg
    13201 gcagatcaat tctttctagc attttgaaac cggcttcgct atggctctga ataaggaacc
    13261 actcgttttc ggtgagccgg cccggcttac taagaatttc agttgggaca gaaatctttc
    13321 caatatcatg aagcaaagcg ccttctcgca ccgtgtctat ttgttccgga cctagaccca
    13381 tctcggtagc aatggctgtt gcaagtttgc tcacccttct ttgatgacct gccgtgaaag
    13441 ggtctcttac ttcaacaact gtagaaagag tttctataag acctctaata gttttttgag
    13501 cggattcctg aatcacatct tcgaacttga ggccacttag gcagtatacg gtggccgtca
    13561 cctttcctgc tagtgatgaa tcttcgatcc tctttactgc ggcctcgacc aaaacagatt
    13621 gtccattgct tttgtttata gagaagaggt gtctttcacc aatttcttga cccgcggatg
    13681 gtttctcgct cctcgagtca gactggtttt caagaaagta gttgatattc tttcctatca
    13741 ggccgcctct ttctcttcct aacagaattt cagccgcctt gttgacaaac acgatttttc
    13801 cgttttcatc aaccgaaatc accgcttgat cgatagtgcc tgccaagtac cttgccgaca
    13861 acagttccat tcgaagattc atttcggcgg agacttcttt atgtacactt atcaaatctc
    13921 gatcagaaag tggctttatc aacacctctt catagaagcc ttcttcagaa agaaaaagcg
    13981 cttcctgtct cccggttcgg gctactctta tgagtgcctt caagaatagg tggtcggcct
    14041 tgctcattgg tctatccagg ggtatgccct ctattactgg aagtcgcgcc agacgattga
    14101 tctcaaatgc gaggtttttt gccgccggga tgatcttttc gcgagacata tcaacagtga
    14161 aaatagccat tccagaagta ttctcttcaa aagctttttt gtaaaccctg aaatgatcga
    14221 tttccgagag gatactgaat gacgtgttcg aatgcattga atgaagcgtt gaatggtcat
    14281 ctattccctg gaagaacgaa gacgctgagg ttttctctcg tttcgtgaac gatgaaccaa
    14341 tacttagact attggtcctt ctgaatatct tgatttcatt ggagtcggat ttgtctgaag
    14401 aagaagagac tctccaaact actccttcgt cgtcaacgaa aatatcctcg cagtcgaaga
    14461 aggctttgta tagtttgccg atcaagtccc caaagtgctt ttgtgaaacc gagttttgga
    14521 agactactct accattcttg ttaacaacta cgaccgcttc atcgataaga tcgagacaaa
    14581 attgggctga atcctccaaa gacaaaactc attcatctct cattctaaag aaaaataata
    14641 acaaacgttg gttcaaaaaa tcttctcgcc tctaataata accagtattc atctaattct
    14701 aacactattc cagcgttttt tggaaagaat aatcaactgt ttgttaatga ctttctcgtt
    14761 tgagtaaacc atcgcacgtt cgataaataa atttaggcct ttcttggtca tctttttcca
    14821 gagaagaaga tctcttctat ggctaaagca acaccatcat cattgtttga ttgagtcaca
    14881 taatcgcaga cggccttcac ttctggaaga gcattctcta cagctacaga aagcccgact
    14941 ctctttagaa ggggaatatc ggcatagtga tcgccgataa atgcaacttg ctgactggca
    15001 agaccatgat aattcaggaa gtcatcgagt gacagccctt tagagactga ggcattaaaa
    15061 agagaaagaa gtccccagct gtcaaccttt gacgctattt ctagacgaac agctgtgtcc
    15121 tccatttgcg accaatacac atccaaaaag cctgcatcat ccgacgctga tatgaacgag
    15181 aagttgaagt cgccagtgta tttagacgtt agcctgccta agatctcttt caaagattca
    15241 agatcgccag tgatgtcgag ttgaagaagg ttcgcactct taactacttc cgtaagatcg
    15301 tccagtctca ctattctgtt tatgttgctt tctaggaatg gcaagtaagg actaagagca
    15361 aagtcgccca catgatacca gtcaggaatt tcagaggaat ccgttctaag gaggagatga
    15421 cagttcagta ttgatgcaag agaaactgag tattccgtta tctttctttc tatgcatcgc
    15481 atgaggaaga tttctccctt tccctttgca agaaaggctc cattctgaag agcgtgagga
    15541 ccctcaatgt ttagaacgtc cagatattca cgagccatta agtagtcacg tccggtaaag
    15601 atcgctagct ttattccttt ttcctgaagc ctctcgattg cattcaaggc tgatgcggga
    15661 attctcttgg aattgtcaag taacgtcccg tctagatcaa gaatcagagc tttcagttta
    15721 cttgcatcga tttcagtgtg accagatctt ctcaatactc aattccctcc cttgccaaaa
    15781 ctcctttaga ataatagtgc ttgatttctc tcatctcagt aacaagatcc gcttctctaa
    15841 ttatctcggc tggggcattc ctgccggtca atactagttc aacctctttc ggtttcgaaa
    15901 gcatgagtct tcggatgtcc attgaagata ccaacctgta atgaaaagca ataaaaatct
    15961 catcgagtat aacgaccccg aattctctgc tggagattac ttctctagat ctggcaagac
    16021 cttttcttgc aacctcaaga tccttggttt ctggatctct aatgataaaa cattcccttc
    16081 cgaactgctc gatgacgata ttttcaatgt agttaggcaa ggatagctca ctgtatctca
    16141 ttccctttat gaactggcct atgaagactc tctcacctgc aagagcagct cttactgcaa
    16201 gtccaattgc cgccgtagtc ttcccttttc cgttacctgt gtaaatttgc acaaagcccg
    16261 tgttcatttc tgcctccttt agcttcagta ttctattgta cttcattgaa agaatatgaa
    16321 tattcttcat cctgctagag ttaccaagtt ggaataaggc cgcaactatg tgataacttc
    16381 gtacatttat cattttggag aagacccttc ttcgatttac agtcggttgt caggtctgat
    16441 cccgaaaaag cagcggtttt atttatagga agattgtgaa ctaaatcaca ttaaggaggt
    16501 gccaagatgg caaagtatga agtaacaaaa tcagttgatg taagaggaga agtctgcccg
    16561 gttcctgatg ttgaaacaaa aagagcgtta aagaagatga aatcaggaga aatcctggag
    16621 gtttggattg actacgcaat gtcaaaggaa agaattccgg aagcggttaa aagtatggga
    16681 cacgaagttc tcgaaatcga ggaagttggg aacagcgagt ggaagatata catcaaggta
    16741 aagtgaaaaa aaggggtgag atgacatggc ctggacgggt ctcttagttg gtctcgtttt
    16801 tggaataatt cttcagagag gaagagtgtg ttttaactca gcgtttagag atgttttgct
    16861 cttcaaggat aactaccttt ggaaattagg ttttcttgct gtcggtctgc aaatgatcac
    16921 agttctcttt gttgcccaaa tgggttggat aaagattgca ccaccaacac tgaatctttt
    16981 cggcaatatt gttggggctt acgtatttgg cctgggtatg gtgctagccg gtggttgtgc
    17041 gtctggtgta acctacagat cgggagaagg aatgacaact gcaatgatcg ctgcggtttt
    17101 ctacggaatt ggtgcaatgg caatgcgggg cggagtgttt tcgcccataa gaacctgggc
    17161 ctctcaattt aacgtttccg tcgatgccaa ttcctctgtc taccttgaca aggttggtcc
    17221 gactcttgca acggtcctca atatcaatcc ctggatccct gcagtgatac tagctgcggt
    17281 tcttctgtgg tatacattgg ggacaaagac aacagagaga aagactaagt tcaactggaa
    17341 gggtgctgcg gtttctcttg ctattctctc tcccattgca tggataacaa gtgaggctgc
    17401 agggagaaac tacggattcg gtatcaccgg tggctgggtc tcaatatttg attcttacat
    17461 cagcaatcag cctcttagat gggacggatt cgaaataatc ggtatcattc ttggcgctct
    17521 catcgcttca cttcttgcca aagagttcaa actgagaatg cccaagaatc ccaagactta
    17581 tcttgttgtg atgatcggcg gcacgatgat gggagctgga gccagtctgg caggcggttg
    17641 taatataggt cattttcttg ccggtcttcc aactctcgca atctcttcta taatcgctag
    17701 tgtgttcttg atacttggca actggacaat ggcctatata ctatacagaa agtgaggcgt
    17761 ctcttttgag aataacaatt caagttttcg caccacctta ctcatatgag gatcttgatt
    17821 cagcaataaa gattgctgaa gccggcctta acaagggaca tgaagttacg atatttctat
    17881 ttgcagactc aatcctttct ataaacagta aggtcaagcc tataagagta gacagagata
    17941 tccccagaaa attggaatct atgataaaag aaaaaggttt gaaagttgag atctgtggaa
    18001 tatgcatgga ctacagaggt gtaaccaaag atatgattat cgaaggttcg aatcccagtg
    18061 gtcttcccga actggcttca ctgattttca acagtgacag gtttgttaac ctgatggcgt
    18121 gaggtgcttt atggataaga agatactttt cgttgtatat caggcgccag caggttctat
    18181 ttgggtaaac gaagcattta gaactgcgtt cggaatgtat ggcgaggata ttgaaccatc
    18241 cgttctccta atggaagagg caactgttgc tctttcaaag aaaactaagc ctgaatgtct
    18301 tggcctactc tcgatttcca tgtgttttag gtttatcaag agatacgaga ctgcggtata
    18361 cggtgtcaga gaacatgtgg agagattcaa agttaaggag atcgaagaaa gcttcaatgc
    18421 aaagctgatt ggagaagctg atcttggaga cttcttccac gattttgaca acgtagtatt
    18481 tatgtgaggt gtctggtatg catctaatag tagtgaagaa cggacccaat aatcctgctg
    18541 aaagagtaaa gataagagca tccaaagaag gggacagtgt tgtcctcatt caagacggga
    18601 ttttctgggc actgaatgac atagaaactg aagcaaaatg ctttgccatc aaggacgatg
    18661 tggaggcaag aggttacaca gccgatgacg ttactgtgcc tcttataagt tatgatggct
    18721 tcatagatat aatcgaaaag tgtgacaaat caatcggtta agctctgaat gtgtgatact
    18781 tgaggtgcgc ttcacaaaga ggtgcacctc tttctgcagg aggaattcaa ggatgcctct
    18841 taagcttcca gatcttttta gaactctctc aaatcaaacg agattagaaa tccttacaat
    18901 gttgatggac aattacctta ctgctacgga aatcgcgacg ttgcttcaaa tagatctgtc
    18961 aactgtttac agacatctga agcagatgaa aaaacttgga attctaactt cgactcatct
    19021 acacggagtg gagagatttg acttcagttc tccgcacatt tttagaatgc tggatgaagc
    19081 aattacgttt atgggtgaac tgaagggatt cagcccgatt gtttgctcgg agggcatttg
    19141 cagttattat cttggcggag agttggacga aattgaacct gaccagcttc tcgatatgag
    19201 aggcgaatcc tgcccagtgc cagatattca agctaggaag acactaagaa aaatgaatcc
    19261 tggcgagatc ttgcttgtta tagtggatta tccactttct ggggaaagaa ttcctgcatc
    19321 agttcagaaa gaagggcatg agtttttgaa aaaagtggcg gataattacg gcgatataaa
    19381 gatctacata aggaggagag aaaatggttg atgccgttgt gattggtggt ggaccaggcg
    19441 gttatgtatg cgcaataaga atagctcaac ttggcaagag tgtcgcactc gtagaaaaag
    19501 agaatctcgg aggcacatgt accaactggg gctgtattcc aacgaaggcc atgttgacct
    19561 ctgctcattt atatacggag ataaacgaga aatccaagag gcttggaatt gatgcatcag
    19621 gactaggcta cgatctaaag agaataatgt cccacatgaa taggacgatt acaatgtcaa
    19681 gaaagggaat cgaacacctg ttgaagaaaa atggtgttga tttctacaac gacgttgcag
    19741 aaattaagga tgcaggacac gttctgctaa agaaccatgg taaaactcta gagaccagaa
    19801 atatagtcat tgccgctggt tctgaaccct ccatattcaa gccgttcagt gaaatcgaag
    19861 ggatttggac cagcaacgat gtctttcaga tggaagagat gccagaaagc cttgttatcg
    19921 ttggtggagg agttatcggt gtcgaatttg caacattttt tagctcattc ggagtgaaga
    19981 ctacaatcat tgagcttgcg gaccacattc ttccatacga agatagggat gtcgccgatg
    20041 acattcgaaa gtcattgact cgacaggggg ttgaaatagt tgaaagaacg aaggtgactg
    20101 aggtggaaaa ggatgaatgt atcttcgttc taaatgccga aggtgaacag gagctctcag
    20161 ttcaggctga gaaagttctt gttgcagttg gtaggaggcc gtatataacc gaagatgtga
    20221 gaaagcttgg tctggaaatt gaaaggggag tcgtcacgaa cagccgaatg cagacaaaca
    20281 ttaaaggtgt ttacgccata ggcgatataa gagccggaat gatgcttgcc cacgttgcta
    20341 gctatgaggg tatagtagcg gctcacaaca ttgccggaga agtaatggaa atgaattact
    20401 ccgcagttcc ttcaatcatt ttctccaatc cggaggtagg atctaccggc ataaaagaag
    20461 acgatgttga agatatggag agagtaatta tcgctaaatt ccccttaagc gccaatggta
    20521 gagcaaggac tgtacttgaa aacactgggt tcgtaaaggt aatcgcagat aaggaaaacg
    20581 gaaaggttct gggaatgagc atagtctctc catctgcaac ggagctcata atggaaggag
    20641 taatagcagt caagaatggg ctaacggtcg aagagcttga gaactcaata caccctcatc
    20701 caacactatc agagacagta cttggtgcgc ttgaaggcgt aaatggaatg agtattcata
    20761 tctgagatag ttcgttaata gagggcatcc ctttgcaggc aatgcttcgg gatgcccatg
    20821 tttattttct gatgtccgaa atcatattat gcttttctgt aaaacctcga ttccttgaaa
    20881 acatctttcc gttttttttg cagggtttct ttcatcgaat tttgttgcac taacctagat
    20941 gattacaaat aaagctttct gaacatccgt tagaactctt ttgaatatat atttctcgtc
    21001 tggctgaaga cctttgtctt ggcgaaagta tgcttataga ataaaagatc caacttgatc
    21061 attatatggc cttcagtctc aaaatcaagc tcagccttcg attgatttac ttttgcagag
    21121 aaacccatgg gaatttccat ctccggacga aggacacaag cgatagttac tcccggaaag
    21181 aacttgaagc caaactttcc ttcaatttca ggagatccgt cgaaatcgat cttatctaca
    21241 aaggttgaat tatggtctat agaacccctt gtatagcttt ttccatgggg cccgtggaag
    21301 tctatcttcg cttcgacttc gtaatggaac ttcggataaa cctctatctc tggaccgaat
    21361 tgaagatcaa tgctctcgat ctgcgtttcc attccataga atagctcaat cggtatgctg
    21421 acttttacgg gaatgccgca tacactgatg cttacctctg gttcagctac cttcttcgtg
    21481 ttttcagtct cgaacccttc ctttccaact atttcgagta tagaagagac ttttgccgga
    21541 aatcgaagag ttccatttga gctgaatttg ttccacgaaa gatgaaagtc aagactgata
    21601 ttcgcgtcga ggaccagttt gttcatgatt gcagctttca tctcatcatc atcaacgata
    21661 gatacttccc attcttttgt aatcaggttt gttgttacac tctctgcaaa ggaacgatca
    21721 ctttcggttc cgaatttctc tatgatttct gatacatcac cttcaatgtt cagaatcaaa
    21781 gtgcccagag cctcttcaac aaaagcttct tccgtttcaa gtgtaacttt tgaagggtca
    21841 gaatcattca cggaaacaac tcttctcaaa gaatctttca ttgtgtcaaa aatataatca
    21901 ccatttgaga aattaggcaa agaagatgtc ttggaaaata cgatttgatt ttcgattgca
    21961 gagggttcct gaacaagaag agaagagttt atcacaagcg atctaggtat agaattgctc
    22021 actgcaatta cgccactggg aaatctggtt gaggaaacca ttggaggttg ctgaactctg
    22081 aaaaggactt ttctgaagga aaccgggccg gaactctctg catcgggtat ctcgtgcctg
    22141 aaggttagaa gatacccact gcttatatac gggtcctgca taccggcggc agaatggtat
    22201 gagactccag agaagaaatc agcatcggat gagctcgaga gatcttgaga acctgcagac
    22261 accggctgaa aaactgagtc aacatgcttt acaattgcac cttccgaatc gtaaatcaga
    22321 tactcatatt cgataccagt accatctatg gatttgatgt aaagataacc ataatttctc
    22381 tccgcaatag aagaaccttc ttcaggtgcc cactccattc taagatcctg gaatgcaatc
    22441 agggttccct ctcccaaatg cgaaatcgac atatctgaag gcgaaaagag gagaccccct
    22501 ccataataag caccgccctt aagttcttca aagggcaccc tgactaaaac cgggttgtct
    22561 actacaatgc acgaagaaag ggaaaagaca acaatcacag atgcgacaaa cagcaaaaac
    22621 gatctagaca agtttttcat tgtccacctc caagaaaaag taaaataatg gatactcatt
    22681 attacggaaa accaaaatga ctcttatgta tttttatttg tccattctat gatttctatt
    22741 ttgccagaaa cattcgggat ttaaccaaga aggtttaatc aattgattcg atctaatgct
    22801 aacatatatt tgtgatcttc tctaagctct ttgaggatga agatagccac aatatgccta
    22861 ttagaaagct ttttattgcc agctcttgct ctcgaagtcg cttactccct tttccgcaaa
    22921 gtccttatct ggaaactctt cgatttcttt cgcttcatca cgtgtgtcaa ttgtatagta
    22981 agcttcagca ggtgcaaata cgcatatctg agctgccctg taagttcgaa atctcattcc
    23041 acatttatca caaagaccaa tatcctcaac atccctcttg aaattcactt ccctgttgta
    23101 tcttcggcag aaggatctgt tcttttcgtc tgtgaagaag tacatggctt gcggctgcct
    23161 ggagttctca ccaccagaca gctcggcctc ctcaaagttt aaccacttcc atgacgtcga
    23221 gttaagtctt tccagatcaa gatcagtttt cttcaaaata ttcacctctc gtgacacacc
    23281 ttttcaaatg aaatgttatc atgaatgtac ctaatacacg agggggaatg attttgaaga
    23341 agtttctact aatactcctg gcagcgtttt tttcgctcac ccttatcgga gcagagtatg
    23401 cagtcaggat aacaaaaggt ggagaagcag tttctaaaga gttctggata acaagagagg
    23461 aaatcgagca ggcatttaac gcaacagtgg caaatgcggc gagccaaggg attattcttg
    23521 atccttattt tgacagttac tttacaccaa gcgagcttgg gctgaaaacc atgataatac
    23581 catacatcgt tgatgagaag ctaatagatt actttgcttg ggagagcaat ttgattccat
    23641 ctgaagagga aattgacgcg gaaaccgatt cgatgatgga aatgtacacc tcgagtcccg
    23701 acatggttga acaaatagag gcgatctatg gttccatgga cgctttcaga tcggaaatca
    23761 gaaactatgt ttccgatgcc cttaaagctg agctcgttca agaatcggtg gctcctttaa
    23821 atgacgacgc tctggcggct tatttcgaag agttcaagac ggagataaag aatcagtttg
    23881 agacgataag agctagacat attctggtta cagaagaggc tacagcaact gaattaatgg
    23941 acagaatcaa cagcggcgaa atcaccttcg cagaagctgc actccagttt tcgatagact
    24001 cttcaacggc tgcgaatgga ggcgagctgg ggtcaatagt cagaggtcag accgtacctg
    24061 aattcgagga agctatcctt gctgctccta tcggcgagtt gtatggtccc gttcaaagcg
    24121 aatttggtta tcatttgata attgtagaag agagaaatga gataaacagc cttgaagatg
    24181 ttgtcaactc ggccagctac aacgatttcg tgtcaggcta tcaaaacgat acttataaca
    24241 ggtggataga gagctatata gaagaaaaca agttcgacta tgagatcttg gataatgagc
    24301 tccttttcta caatgaatat gccaaagcaa aagcgacaga agagactgcg aacgaattct
    24361 tcgttgagat agcgtcgggc atatttggcg atggaacctc gtccgaagca tccttgatgg
    24421 aatatgcagc atttattgaa ctatcggaaa tgcttggctt tactgacagt ccggactacg
    24481 agacagcgat acgaaatctc tttgaagccg gtgagaagcg cggtatgatc gtgcagaaga
    24541 tgtatgatct ggatagtgaa aaccccgagg ttgctgcagc atactacaat gcattgctgg
    24601 aggaacttga aaacacgttc atgaaccagg atcttcttca gcaacagtta agcagctatg
    24661 gacagagctt tgtagattac gtattcaaca caattggaga gattgaatcc ggtctaacca
    24721 gcgttcttga aaaagagatt tccgatggac ttagagcaga cgttctatac attctcataa
    24781 gaaataactc cctttccctt gagctcgatt acagtcccga ctggaggaag gagaagctca
    24841 atcaaagact cggttactta gaagctttga tacaagttga gccatctgaa gctgctcaga
    24901 cggagataga atctataatc tccgaactgg agcaaatcga agctgaggct gagactcctg
    24961 cagcaacaga atgagcttgg ctttttaaat aggagagccg gggaagttgc cccggttctt
    25021 tttttgattg tggcagactt tcatcttttc agtttttgcg ccatgcggat acttctatcc
    25081 cgtcgttttc tcaggcaatt tcctccgcat taccataaga gtctcctccg tgttttcccg
    25141 gcctcacttc aaagagtctc gacattcctt ttgccaagaa gacaatggtg agaatggcat
    25201 ttgcaataca cgatatcaac acaagatagt gaactggaag cctgtcaact gcgaagccaa
    25261 agaccgctga accgagaggt gttgcaattt gcgctagaat cgaaattacg gaaaagaccc
    25321 tagatctgtg gctggtagga agtactcgat gaaaaagtgt ttgaagagga gtattcacaa
    25381 atgcgttgaa cacacccatt acaactatcg gtattcccag tgcggcaaag aacaaccaag
    25441 acggtccacc gaaaagactt gtgaagtaag gaaagaagag agcggctaga aggaaattta
    25501 agactgtctc tgttactaat ccctccgcaa acagtttgtc ctgtttctta ttcacgagaa
    25561 tcgtaccaat tagaatgttt ccaaaaagga ctcccaatac ccaacccgac tggagaaaac
    25621 cgtattgttc gctagagaaa ccaaccaatg tgcgggcgaa aaaaggaaaa acaacggtga
    25681 aaatcggagt tgacaaaaaa ttggaaacca ttgcaaacat cagaaccatc atcaagccat
    25741 ctatcttctt taaatagcca attccttcaa ctatatcgac aaaaacgctc tttaccgaga
    25801 cgcgcgtttt tttagtagtc tgctgataaa ctatgaagat ttcactaatc gcagaaagaa
    25861 taaacgatgt tccatttata atgaagacta tttcaattcc gaaaagtcca tatagaactc
    25921 cgccgagagc aggaccgact atgtaggaaa cggagttaac cgcgccaaga atagagtttg
    25981 ccttcagaag tttgtgctct tcaatgatat ctggtagcat tgcagaagtt gccgggtcga
    26041 atgaaatgtc gaaagtcgat ataactagct ggaagacaaa gagaagcgct agattcaaga
    26101 caccgacatc tgagaggtat gccataagaa taattgctgc tcctctagca aaatccatat
    26161 atatcataat atatttccga ttgaatctat cacccaaaac accggcgatt ggtccaaaga
    26221 ggattctggg cagcatcgtt actacagaga atgtgcccat catcaccccg gagccagtta
    26281 gatccaaaat gtagagtggt atcgcaagcg cttgaacgcc gcttccaata agtgacacta
    26341 gtcttccaag agtgaaaagc cagaaatttc ttcccaaccc tttcatcgaa ccacctcaat
    26401 ttctgcaaat gctggtttaa tattatcacg tttaacgtca aaacatcaag atctacctta
    26461 aattattgat attagaaatt gagtagacac aacaaacttt attatttcgt tcaagaaagt
    26521 agaattgaat cccgattggg ggaaaagtct tgtcagatct tcaacttcac cggaggtttt
    26581 gatctgccgt gctatcatct agtaattatg aattggaagt tctattactt tttagaagtt
    26641 gcagcaggag tgttcataga ttttggttat cagtttatga agtaggagga ggatttttat
    26701 gtctagagta gttaccttcg gagaaataat gatgcgtctc gcatctcctt tgggagagag
    26761 atttgagcag accaagaatt tcgaagttgt ttacggggga gcggaagcca atgttgctgt
    26821 tgcgattgcg aagtacggcg gaaaaagtag attcgtatcc aaagtatctg ccgatcagtt
    26881 tggagacgcg gcaattggga atattggtgt tcacggggtg gatacgagtt atgttttgaa
    26941 agacattggc aggctaggta aatacttcta tgaatatggc tattctcaga gaccgtcaaa
    27001 ggtaatttat gaccgtgaca actcggtctt cgctaattca aagcccggag atttcgattg
    27061 ggaagaggtt ttctcagatg ccacctggtt ccacttcaca ggcattactc ctgcgcttag
    27121 cgacgggttg attgaaacat gtgaagtagc tctaagagag gccaagaagc gaggaataac
    27181 tgtttcttgc gatctaaatt acaggtcgag actatggagc aaagaaaagg cgagaaaggt
    27241 tatgacttct cttatgaata acatagatgt cctttttgca aatgaggagg atagtgaatc
    27301 tgtctttgga ataaaggctg agggcactaa tgtgagtgat ggcaggttaa gtgtcgaggg
    27361 atacagagat gtggcaacaa agcttaggga cacttttgat ctgaaggcag ttgcaatttc
    27421 cctaagagaa agcataaatg ccaatttcaa taaatggtcg gctgcgctgc tcactggagg
    27481 agaatttatt gtatctacga aatacgacat aaacataatc gatcgtgtag gcggaggaga
    27541 tgcatttgct gcgggattga tttttggact ggacaattca tgggaaccat tgaagtcact
    27601 tgagttcgga gtcgcagcct catgcctaaa acacacaata aagggagact tcaacatcgt
    27661 atctagagac gaggtcgaaa agctacttgg tggaaacgct tcaggaagag ttgtgaggta
    27721 ggaatttgac atcccgccat tatctgcaaa gttgtgacgt atgcggtttc tagtgcagca
    27781 attcagttgg tatcccaaaa tgccctaagt gtggcagcac tctgaattac agatacaatc
    27841 aaattggcgg aatgaatctg gatgattttc cggacaaata ttgttacggc ttgtgaaagt
    27901 accatagact actaccaatc gatgatattc gccattgtgt aacgctggga gagggtgcaa
    27961 ctcctttaac tgctggcgtt agacttcagg ctgagttggg aaacaccggg agaataatgc
    28021 tgaaagacga aactcagaat cctaccggca cttacaaaga tcgccctgca gctatgggtg
    28081 tcagtaaggc taagaaaatc gcttcaggaa aagtaatcat cgcttctgat ggaaacgctg
    28141 gacctgcaac agccgcttat tgtgcgaaag ctggactcca ttgtttcgta cagatgcccg
    28201 ccgatacgcc tgttgaaaga aatgtgcaaa cgatcttata tggtgctgaa ctaattcttg
    28261 tggaaaaaag caatgtgagt gattgtatag acatgataat ggatctatct gaatcttcta
    28321 gctggactca ccttacaact gcctcatcag tcattcctta tcactttgaa ggtacgacca
    28381 tagctttcga gattgctgga gatcttggat ggaatactcc cgattgggtt atgatgcccg
    28441 agggaggggc cggactatca gctgctattt ggaagggctt tttggagatg agcgaagctg
    28501 gtatgatagc caaactccca agactcctag ttgttcaggc tgagggttgt gcgcctctgg
    28561 taaaggcctt taaggagcac gataccaaga tcgagagatg ggaaaagccc agtactctgg
    28621 cgaaagcaat tagcgtgcct tacccgcttg acggtgaact tgctcttcga gcaataaggg
    28681 attccggtgg gtcggctatc tccgtgtcag acagtgagat ggttaacgct acccgtgtca
    28741 tggcaagaca cgagggtatt tttgcagaac caactggcgt ggcttctata gcaggtttgg
    28801 cgaaagccct tgaagaggga atcgaaagta aggaagaatc agttgttgcg cttgttacgg
    28861 ggacaggact aaaggtcatc ttatcattta ccaagcttct tgaaatacat aggacgattg
    28921 gaaagaacat tttggatctg aagagcattt tagaaggata aagaatagtg tgttctgcac
    28981 ctcaaatact ggaacccact ggttgcatcc agtaaggccc tttctcttta gt
//
LOCUS       DAOQYI010000022        28861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1901_length_28861_cov_75.765049, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000022 DAOQYI010000000
VERSION     DAOQYI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 28861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28861
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1901_length_28861_cov_75.765049"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKL93_06950"
     CDS             complement(<1..200)
                     /locus_tag="PKL93_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HNQ71032.1"
                     /translation="MKKIILVFLSLFLAISLFGIVSYDLQKVIIVPEEPQGGLEVSIW
                     LDRDIGSLYYSGEEVKTYFKVN"
     gene            complement(226..1737)
                     /locus_tag="PKL93_06955"
     CDS             complement(226..1737)
                     /locus_tag="PKL93_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HNQ71033.1"
                     /translation="MAEVPVRPKKPRRRRRNEKQKNVISLGNGIVIVVFVVGLLMSLL
                     YIPWRVSLDSKYNRAALVTEKKVDGVPSKQLIYVDNLAEISELGSAVIFMDLRDDWNR
                     LEEILDLQVPVILTGVSPYPVSELASILDSHCAYTGYMEFDERGQYVLDVLKARDNKS
                     LVFRVHNLKKKEYPNYDIDRAVTRYIRSVRERSVDALLFLTPPVDFDYDELVHKSYEE
                     LNEQDLISDEITSPRTGSSRFKLLSALFIFVLILSVSPLGAAGITVVFLLFPTIGLPL
                     AAIAGEFAIYRRLSSLDTGVFRGLLLFFSLSVFLGISINASMVGVEFQNGLELFRGVK
                     VSLVALPGWLFVTGFMKSVSRKISKGDLLVFVFAGIAAGYYILRSGNFSFVLDSERMV
                     RDYLDNLLLVRPRFKELLAYPLLAILIHFSFNIKGKLAPVIASGGSLVIVSIVNTFCH
                     ATVPLWTGLLRSLYGLLFGTVVSMILITFVKKYNTSSKDVEVLLNESDEDESN"
     gene            complement(1668..2438)
                     /locus_tag="PKL93_06960"
     CDS             complement(1668..2438)
                     /locus_tag="PKL93_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71034.1"
                     /translation="MPRIRFVFLIIVVVFSSLPVAGQAEIRSEIEEANAELSEIYGFS
                     VKYSLTILKGEGHEQPAAIDNNGIYQIFLYTSSYKPGVARHELAHVYFFEYLRIRDIN
                     PDEIPLWYHEVIAEGFQNLHTGTRRSALRVAFFDFTEYEERYPVKDDQSVFYGAVASF
                     ADYIIKRNSYNKLLEVVNEFSTEREIDTAFLNVFGSTVRSMLLKWRIVFLLPYSPFVV
                     GVVLFLYLLIGRRDKYWRKFPLDLRSLEEDEETKNRRM"
     gene            complement(2426..3364)
                     /locus_tag="PKL93_06965"
     CDS             complement(2426..3364)
                     /locus_tag="PKL93_06965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNQ71035.1"
                     /translation="MNFLRGLNAPFNLSGVISIEKEFKVTKENFYERLDRVLRKELSD
                     LKLSSIYKLLRRGNVRVNGNKIRDGSWKMEIGDKVQVVFSGDPSKLKRLEESRELTPK
                     HIPLDILFEDDLIVAVDKPSGISVHPGKGIQIITLVEGLMAYGNSKGFVPRPVHRLDK
                     HTSGVILFAKSPEAARELSKLFRERGVEKGYFTLVKGFPEKRGKLVSQREKFVESLAF
                     TVEKSYATTSLLWIDLFTGKKHQIRRQLSEAGYPVVGDDVYGDRLFNRDFKKETGLRR
                     YFLHCSRLSFARNDGKEIEIISELPGDLKRVLGCLE"
     gene            complement(3361..3612)
                     /gene="acpP"
                     /locus_tag="PKL93_06970"
     CDS             complement(3361..3612)
                     /gene="acpP"
                     /locus_tag="PKL93_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNQ71036.1"
                     /translation="MTVDEVFDRVKTIISEKLGVEEDEIAMDSDLTEDLGADSLDLVD
                     LVMALEDEFDFKVEDEQIENISTVGDIVESIGKGLGVED"
     gene            3749..4573
                     /locus_tag="PKL93_06975"
     CDS             3749..4573
                     /locus_tag="PKL93_06975"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNQ71037.1"
                     /translation="MRLYTEMKETVVRRLTTFPKVAMILGSGLGYLTEQFEEPLAIDY
                     REIPGFPTATVEGHSGRLVFGTFQGLPVVAMEGRFHFYEGHRIEDVSSPIYLFKELGV
                     SNLLITNASGGINRSFSPGDIVAVTDIINFGFRNPLRGENDRRYGPRFPDMSEIIDFA
                     WLKVLKSRLEKAKIDLKEGTYCWTLGPSYETPAEIRAFEFFGADLVGMSTVPEVIAAR
                     HCGIKLLVLSCVTNMASGILKEKLTHADVVKTANRIRPKFTAVVQHAVESIKETEQ"
     gene            4570..5556
                     /locus_tag="PKL93_06980"
     CDS             4570..5556
                     /locus_tag="PKL93_06980"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNQ71038.1"
                     /translation="MIRKITSIVSCIQKANDILVVGHIMPDGDCISSVVSLSMGLEKF
                     GKKVTPAIDWKIPANFTVFPWIDRIKDFSNDVVEPNLIIIVDASSPDRIGRFEQLLRK
                     GIPSVVIDHHATNTYFADDCWVDPSYSSAAQMVLDLLKLMDVEYDADLALMNYIGIAT
                     DTGFFRYSNVDSSVFEAAAELVRLGADPTFVATSILETRKIEELFLERDAIDNIKMLS
                     NGRFAYSYLELEDFEKYSLTEDDFVGFVGDLRSIKSVEVALFASEASKGEAHVSLRSK
                     SYFDVSKIAVAFGGGGHQKASGFTLRYDGNLEDALAETIEMIDSQLQQKEKH"
     gene            complement(5553..6188)
                     /locus_tag="PKL93_06985"
     CDS             complement(5553..6188)
                     /locus_tag="PKL93_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNQ71039.1"
                     /translation="MFEMMRNFFCLTPAIAAAILSHEYARFITARRFEATKPEWGGPG
                     FIRRIDPVGLLMFYFFKFGWSRPFPVNYWKLRKAKYFKAILTALSGSIANFSLGVITG
                     LIFYLSGLHRFSTFMPETVSSFPASYLADVVYWTMVINLNTALFNLIPIPPLDGANIV
                     TMLVPESQVNWLVKYELYGILTLLVLSLMGIIQLIMWPITQFIQLLARLIA"
     gene            complement(6181..6885)
                     /gene="rsmI"
                     /locus_tag="PKL93_06990"
     CDS             complement(6181..6885)
                     /gene="rsmI"
                     /locus_tag="PKL93_06990"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNQ71040.1"
                     /translation="MTEKKGKLWIVGTPIGNLDDMTIRGKRVLEEADLILAEDTRRMR
                     SLLSSLGIENKDVVSLNMHNQEERLSFIIRKLDEGAKIALSSDAGMPVISDPGAKIIR
                     ICRDRGFEVDVSPGPSAVTSAIAISGFPGSHFTFLGFLPRGKNRRRLLRKISRGSYGE
                     SLIVFFESPFRLMETLREILEIIGDREIFIGRELTKLFQESFFGCVSDAIQKFAFSEV
                     KGEITVVLSGRDEEDV"
     gene            complement(6882..7559)
                     /gene="rsmG"
                     /locus_tag="PKL93_06995"
     CDS             complement(6882..7559)
                     /gene="rsmG"
                     /locus_tag="PKL93_06995"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNQ71041.1"
                     /translation="MVFSSEFLEPSFFAKYEKLIELMLSSPHNLSSIKEFERAMTVHI
                     EDTIIPFEGSSLSGRCIDIGSGGGIPGLVLAISFPKSNWTLLESIAKKTREIERFARD
                     LDLTNVAVKTARAEEFAKEAGETFDSAFLRAVGRCDISLELAAPLVKVGGYIYLYKGP
                     GWLDEKVFSDAAERVLGLRLSYEKEYRLSEGSKRFMVVYEKERATPSEFPRRSGVASK
                     LPLGGRK"
     gene            complement(7564..8328)
                     /gene="folE2"
                     /locus_tag="PKL93_07000"
     CDS             complement(7564..8328)
                     /gene="folE2"
                     /locus_tag="PKL93_07000"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNQ71042.1"
                     /translation="MRDVQNEKDKRNIKINMVGIKSIEYPIVVLDRKFGTQNTVGKFD
                     LFVDLPKDFRGTHMSRFVEVLERHHKKITPRSMESILNDMRDSLKADVAHIKVEFPYF
                     IRKKAPISGSESFSSFKCSFIAMKDGTFDFVLGVKVPVMTVCPCSKEISDRGAHNQRA
                     EVFVSVRMNSLVWIEEIIEYVEKSASAPIFSLLKREDEKFITEHSFDNPRFVEDLSRE
                     VVLLLQDDSRINWYRVEAISQESIHNHEAYACIEKE"
     gene            8380..8468
                     /locus_tag="PKL93_07005"
     tRNA            8380..8468
                     /locus_tag="PKL93_07005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8414..8416,aa:Ser,seq:tga)
     gene            complement(8488..9330)
                     /gene="lepB"
                     /locus_tag="PKL93_07010"
     CDS             complement(8488..9330)
                     /gene="lepB"
                     /locus_tag="PKL93_07010"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNQ71043.1"
                     /translation="MSAKKSKKGSDKKESRFLHEVKEWGKAILYAVIFGTIIRLFVFE
                     TMLVPTGSMIPTINPPARLFVEKITYEYREPDYGDIVVFWTPYVDIESQKYLRGFDKF
                     MDLFSPAKYRGHVKYVKRLVGKAGDVLELRRAPDYTAANPVYQLYVNGEIPPALEERR
                     YVREGVFYDPSFYLGLARPDDPSVRFSPYYRLYQAYKGLIEYTEFFETELEPLGLEKY
                     ITQDPSTGVVRVTVPEGFRFMMGDNSANSFDSRYFGFVPEEAIIGSPMLTIWPFSDFG
                     PLKK"
     gene            complement(9337..9684)
                     /gene="rplS"
                     /locus_tag="PKL93_07015"
     CDS             complement(9337..9684)
                     /gene="rplS"
                     /locus_tag="PKL93_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNQ71044.1"
                     /translation="MDQYIRSLENEHLRDDLPKMGPGDTVRVSVRIKEGNKERIQAFE
                     GIVMGIRGSGTGKSFTVRRVGAAGVGVERIFPFTSPSLEKIEVLRRGKVRRAKIYYIR
                     DVKGKVKIKEKRD"
     gene            complement(9653..10270)
                     /locus_tag="PKL93_07020"
     CDS             complement(9653..10270)
                     /locus_tag="PKL93_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNQ71045.1"
                     /translation="MLNNIYLALIHYPVLGRHGNIVSSAVTNLDVHDISRTCRTYNVK
                     GYFVVSNLPAQREIVDNVLNYWLEEFGREYNPSRSEALKVVERASYIEDVLEQIEEIE
                     GQRPDLVFTSAKRGKDRITFDAMRRIIVKSEKPHLLLFGTSWGLPGEIEKICDYALEP
                     LRANSDFNHLSVRAAVAIVLDRLIHEDTVEVRRDKDGSVHKISGK"
     gene            complement(10260..11003)
                     /gene="trmD"
                     /locus_tag="PKL93_07025"
     CDS             complement(10260..11003)
                     /gene="trmD"
                     /locus_tag="PKL93_07025"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNQ71046.1"
                     /translation="MKIEVLTIFPQLFETVFNWGVISRAIKNGIIAFESVDIRDFTDD
                     RHRTTDDYPFGGGSGLVMKAEPIIKAVASRFSDDSKPHVVYPSPQGKLFDNKKALELS
                     KLSHIVFVCGRYEGIDERAMSSIDEEISIGDFVLSGGEIPALLMIEVISRFVPGVVGD
                     MESVINDSFFSDLLDYAHYTRPREVNGMEVPEVLLSGNHEMIAIHRRMESLLRTIERR
                     PDIFLKHDFDLTDKKALLLLFKELKKDAE"
     gene            complement(11000..11557)
                     /gene="rimM"
                     /locus_tag="PKL93_07030"
     CDS             complement(11000..11557)
                     /gene="rimM"
                     /locus_tag="PKL93_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491755.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNQ71047.1"
                     /translation="MSELKDKSLEEMVPIGRIVKPHGLRGEMKVKVALEDETAFKEIQ
                     EVLLYDEKTRSRFVTSIDGARKATKGWILHFEGIDSMGQAERLVGFHIYVEERVLPEL
                     KQGEYYYFQILGSSVFDENRRFIGTVNDIIETGANDVMVVVREEDLTVREELIPIIRD
                     YILELNFEDKTIVARSMEFEEVKPE"
     gene            complement(11554..11787)
                     /locus_tag="PKL93_07035"
     CDS             complement(11554..11787)
                     /locus_tag="PKL93_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNQ71048.1"
                     /translation="MKEVLEHILKGIVREPDRITVTEAVDENGNMVFEISAAEEDVGQ
                     IIGKDGRTIKSINVLLNALAGSSKESFILKVVR"
     gene            complement(11787..12077)
                     /gene="rpsP"
                     /locus_tag="PKL93_07040"
     CDS             complement(11787..12077)
                     /gene="rpsP"
                     /locus_tag="PKL93_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNQ71049.1"
                     /translation="MVKIRLTRMGRRNRPFFRLVVVDSQKRRDGAYIDSLGFYDPIRD
                     PGIMSVDVDKAVSWIMKGAQPTETARSILARFGVMKKVHELKYVKESGEKAE"
     gene            complement(12109..13425)
                     /gene="ffh"
                     /locus_tag="PKL93_07045"
     CDS             complement(12109..13425)
                     /gene="ffh"
                     /locus_tag="PKL93_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNQ71050.1"
                     /translation="MFENLQEKLGRAFKLLSGKGRISEKNIEDAVKQVKLSLLEADVN
                     YKVVKEFIGGITEKALGEEVLKSFTPDQQFIKIVRDELIKMLGEKSVPLKLSSQPSKI
                     MMVGLQGSGKTTTTAKLGTLLRKEGRKPLLVAADVYRPAAIDQLMQLGKQIDMPVFTG
                     DRKNPVKIVQEAMQQAVRNINDVVILDTAGRLHIDDAMMRELKEIVSAVKPDEILMVV
                     DAMTGQDAVNSAKAFNEALELSGFVVTKLDGDARGGVILSIRHVTGKPVKFIGISEKP
                     DGIEAFHPDRVAGRILGMGDVLSLIDKLQYNIDEEKAKEMEEKFLKNKFDLEDFLDQL
                     KEVKKLGSIADIMEMIPGAPKDVDLAGSEKSMKRTEAIIHSMTSKERRNPKLLNYSRK
                     QRVARGSGTTIQDVNKLLKSYDQMRSMMKQFGRKGKLLKGMKGFPF"
     gene            complement(13663..14235)
                     /locus_tag="PKL93_07050"
     CDS             complement(13663..14235)
                     /locus_tag="PKL93_07050"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNQ71051.1"
                     /translation="MSGKLTVVVGPMYSGKTSTLLSMIEIYTLGKKRIKVFKPVIDDR
                     YSSDHVVSHSGQMAEAINVYDSSEIKEIVSKEKGRLDAIFIDEINFFDENLLRIVEEI
                     IFSGVDVFCVGLDLSYKHRPFAVTANLMAAADEVIKKKAVCHICGEYNATVTHRISGP
                     TDSEIDIGGMEKYIAVCRDCYKKLNAKDWG"
     gene            complement(14240..15034)
                     /locus_tag="PKL93_07055"
     CDS             complement(14240..15034)
                     /locus_tag="PKL93_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HNQ71052.1"
                     /translation="MPVTDFTERSKFRALVLGGYLEDVEVSGSGHKASIVKTDGGSAF
                     NVATSLSLLGAEVLFLSCFGVCDSDRWSFRSIPVMPDCAHGIFLFSGSDVIAVQKPQP
                     VDVNQELVGILIDEEYDLLYSTLEIGQQAASVISSVKSRIKVVDPSPFHEFKGLDGLG
                     KYDFILANDYMSFEKSDSRLIIKASEKGVNFAGKDYLPPVVGKNRFGSGDIFGSVFSL
                     LLMNGASPEEAIGEAVKVSGEFCSQTRSVEQFMLGIRGSLNYVIGG"
     gene            complement(15003..15578)
                     /gene="upp"
                     /locus_tag="PKL93_07060"
     CDS             complement(15003..15578)
                     /gene="upp"
                     /locus_tag="PKL93_07060"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNQ71053.1"
                     /translation="MRDDQTGPKEFRELLKEITLLLTYEATRHIPTYDKEIKTPLVKM
                     VGQCIEDKKVTVVPILRAGLGMVEGVLSLMPNASVGYIGIYRDPDTIQPVEYYSKLPS
                     VDENTQLFVLDPMLATGVSSSWALKVVKKAGAKQISLMCLIAAPEGVRFIEKNHPDVK
                     IFTAALDERLNDHAYIIPGLGDAGDRLYRTK"
     gene            complement(15628..16917)
                     /gene="glyA"
                     /locus_tag="PKL93_07065"
     CDS             complement(15628..16917)
                     /gene="glyA"
                     /locus_tag="PKL93_07065"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNQ71054.1"
                     /translation="MWESLEKTDKQVFDIMFKELERQRNGLELIASENFVSRAVMEAM
                     GSVMTNKYAEGYPSRRYYGGCVFVDEVEDLARERAKKLFDAGFVNVQPHSGSQANMAA
                     YLAVAKPGDTIMGMSLSHGGHLTHGSPVNFSGKLFNAVSYGVNEETEVIDYDEVRKVA
                     LDAKPSVIVAGGSAYSRIIDFKKFRDIADEVHAVLIVDMAHFAGLVAAGLYPNPLDFA
                     HVVTTTTHKTLRGPRGGMILTNNEEIAKSVDKMVFPGTQGGPLMHVIASKAVSFGEAL
                     RDEFKAYQQNIIYNTRRLAKSLEEKGLRIVSGGTDTHLFLVDLNPMNVTGKAAEKALE
                     KADITVNKNTIPKETRSPFVTSGIRIGSPAITTRGMTEKEMPLIADLIIRVLENIEGE
                     KGEISQTKVREISEEVKTLTSKFPLYSDLIHRSDAGV"
     gene            complement(16918..17985)
                     /gene="mtnA"
                     /locus_tag="PKL93_07070"
     CDS             complement(16918..17985)
                     /gene="mtnA"
                     /locus_tag="PKL93_07070"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNQ71055.1"
                     /translation="MSHFKSLTLEWSEDRLRLIDQRKLPTAEEYVECRNHEEVYVAIR
                     EMIVRGAPAIGATAAFGYLLGAKEVLDASDEVFLDHMTTVKKRLSESRPTAVNLFWAL
                     DRMENALNSNWGLERKKLIDSLERAALSIAKEDIEINKSLGRNGATVVRDGDGILTHC
                     NAGALATVDYGTALGVMRAAVEQGKRIKIFADETRPYLQGARLTAWELIKSGIDVTLI
                     CDNMAGWVMKKGFIDLVLVGADRIAANGDAANKIGTYSVAVLAQRHGIPFYIAAPLST
                     VDLTTASGEEIPIEERSHREITHIGNQQIAPEGVRCLNPAFDVTPSSLITGIITEKGI
                     ARPPYDVSLREMFEENPEEVI"
     gene            complement(17985..18791)
                     /locus_tag="PKL93_07075"
     CDS             complement(17985..18791)
                     /locus_tag="PKL93_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNQ71056.1"
                     /translation="MNPKEMAERMETIRDNVKSIKNKILVMSGKGGVGKTTVAVNLAL
                     ALADEGFKTGLMDIDLHGPNVTRMVGLKKQPEVVEGQIFPPEALPNLKVISISSFVEE
                     DAPVIWRGPMKTTAIYQFLGDVMWGELDFLVIDSPPGTGDEPLTVLQTLTDLVAVVVT
                     TPQAVAVQDVKRAINFVKTMHRDILGIVENMSYLRCPHCGEVIRLFGEGGGKDLENLF
                     NIPLIGSLPFDPALVDFSDTGKSIVTNMRGSELENAYRETVREIVKRVKV"
     gene            complement(18788..19210)
                     /locus_tag="PKL93_07080"
     CDS             complement(18788..19210)
                     /locus_tag="PKL93_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNQ71057.1"
                     /translation="MEYSARRIGKMAFYAKTPSGHDIHMDANESSGGDGSAPAPMEMV
                     MAALMGCTSMDVVSILSKMKVTNYEYWISANYEYAKEHPKVFTSIELVYHFSGKNLPK
                     DKIEKAVTLSQERYCSVSAMLGKTADMSMRIEYEGEEK"
     gene            complement(19253..19612)
                     /gene="rplT"
                     /locus_tag="PKL93_07085"
     CDS             complement(19253..19612)
                     /gene="rplT"
                     /locus_tag="PKL93_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNQ71058.1"
                     /translation="MRVKGGVNTKKKKLRYLKAAKGYRGALSRRYRLAKQYYIRSGVY
                     AYAGRKVRKRDFRKLWITRINAGARIAGTKYNDLIHGLKIAGVNINRKMLADLAVNDF
                     GTFKEYCEIAKSALNGN"
     gene            complement(19636..19839)
                     /gene="rpmI"
                     /locus_tag="PKL93_07090"
     CDS             complement(19636..19839)
                     /gene="rpmI"
                     /locus_tag="PKL93_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNQ71059.1"
                     /translation="MGNKVKMKTHKASAKRYKVTGSGKIMRNQSGTGHNTGKKSESSR
                     REARKWKLVEGGYEKKVKKSLGI"
     gene            complement(19808..20374)
                     /gene="infC"
                     /locus_tag="PKL93_07095"
     CDS             complement(19808..20374)
                     /gene="infC"
                     /locus_tag="PKL93_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNQ71060.1"
                     /translation="MARGDSTPKNNEIMTKTVRLVDMNGEQLGVVQTSEALRIAREKG
                     LDLVLVAPAANPPVARIMDYGKYKYEKDKRKKEAKKKTKQSQLKEMKFRIRIDEHDFQ
                     TKTNRIKEFLEKGSKVRVVIMFRGREIVFSDKGREILERVADQLQQISDIDRPPKLEG
                     RDMWMILKPKTTPQGGKDNGQQSKNEDS"
     gene            complement(20499..20574)
                     /locus_tag="PKL93_07100"
     tRNA            complement(20499..20574)
                     /locus_tag="PKL93_07100"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20539..20541),aa:Lys,seq:ctt)
     gene            complement(20624..21442)
                     /locus_tag="PKL93_07105"
     CDS             complement(20624..21442)
                     /locus_tag="PKL93_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNQ71061.1"
                     /translation="MVKAEMGNTGKIKIGIVQFRADNDAPGENLEKITSYIESLVEEN
                     VDLVLLPEMFNSGYGTDEATISNASEMQEETVEVLSALADYNDIAVVGGIVNKTNGGF
                     FNSTVIMLPYLEPIYYNKTHLFRDEKKVFTPGSEFKTFEFSGIQFGVLMCYEIGFPEI
                     SRKLCKQGAEVLLVPFAFGRERKLIFETATRARSIENGCFLVASSQCGTNKTMNFVGG
                     SRIISPSGEIIVDCGGAEGFASAEIDIRLVKKYRFGESRDSHGYFSNFRDDLYR"
     gene            complement(21429..21914)
                     /locus_tag="PKL93_07110"
     CDS             complement(21429..21914)
                     /locus_tag="PKL93_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71062.1"
                     /translation="MIYRIIFSLFLLFIMPFLNYSIMLSAIVVSLVLIGVILGSKTER
                     VARIQNLTLTLFYVVILFGYFQDTAGMVYRSEVVILAVAQGVSGFYGLFHHRRSLSVV
                     LSLGYWILVGTALSRIAWMRLGSGGLILGIALIALVAFQDIRRIYKPLVRSPFEQDGE
                     S"
     gene            complement(21914..22630)
                     /locus_tag="PKL93_07115"
     CDS             complement(21914..22630)
                     /locus_tag="PKL93_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71063.1"
                     /translation="MKKMLVIIFLIVFSSVFFASEYEALLSEFVQIRAMEDTEMMAEF
                     IEKLESEDLEDIEILTLLADCHREYANWIEDKKTRETHYNKARELAEQAIKMDDSYGM
                     AYYVKGAAIGQLAQMAGIIQSMFLISDFDKSIDKAMELMPDSPFPFIAKGMRDRDTPW
                     PYRNYGKSEERFMKAIENDPGYLNSYYELAVLYQVWKKKDLAAQYYRKVLELELQPDF
                     IVQGQEAKENAQKWLEDNGY"
     gene            22713..23927
                     /gene="thiI"
                     /locus_tag="PKL93_07120"
     CDS             22713..23927
                     /gene="thiI"
                     /locus_tag="PKL93_07120"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNQ71064.1"
                     /translation="MNRFVVVRYGEIALKKGNRKLFEKVLVNNIKRQLGRSTVERIRG
                     RIIVSVPSERLRSSIEIMSRTFGIQNFSVGTWTSYDLEDIYLTSVDLAKDEMKRGRKT
                     FKVETRRLDKRFPLKSVELNPLVGERILEELPETSVDIHNPEFKIEIEIRDEGVLISS
                     GKTTASGGLPVGVSGRALLLLSGGIDSPVAGWLAQKRGLDLDAIHFSSPPYTGERSFD
                     KVLSLAKTLSIYNGGREFRLYSMHFTDAQIAVHKHVEERYSLVCQRRLMMKIASKIAE
                     AQRIIAIVTGENLGQVASQTLENLRVIEEQTELIVLRPLLSYDKIETIELAKQIGTFE
                     TSILPYEDCCTVFVPSNPVTKARLFDINRVEAGLDLECLARDIIEKSKMYRIKNGDIL
                     EVKSIEIPEEAS"
     gene            23902..24654
                     /locus_tag="PKL93_07125"
     CDS             23902..24654
                     /locus_tag="PKL93_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ71065.1"
                     /translation="MRFLKKLLSLLVTVWIAVLGVLYIYVYGGIVILIGNVIGKFKGK
                     KERKTFISREVSRFGRAAFVLSGSRVRLSGKENVPENGPMVIVANHQSAFDIPLIPGY
                     VYPEIAFIAKKELSKIPGISWFISALDGVYIERGNRLQTAGAMRKIFRILKEDGTILL
                     FPEGTRSVAGEIGKFKEGSLSIPYKLGVKVLPVALDGTRNLLRKNSFLITPSTISLSI
                     CKPILPQDFSSEEDFSGEVYNIIKAALESMRN"
     gene            24665..25198
                     /locus_tag="PKL93_07130"
     CDS             24665..25198
                     /locus_tag="PKL93_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNQ71066.1"
                     /translation="MLQVRLRGLALDQSNSPVVILEVEKTNKGFGIWIGPFEAEALAL
                     AISGKDFPRPLTYDLFVNTVTQLGGVFEKAVIGQVKDNVYYASLHLQDRTGQLYVVDA
                     RPSDCLVLAVKKGFPIFVEDSVFKESSIDLSNLQTDALQHGQEEDTEGFKKFVENLDI
                     EELKKHFRSKEDDSETS"
     gene            25185..26024
                     /locus_tag="PKL93_07135"
     CDS             25185..26024
                     /locus_tag="PKL93_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNQ71067.1"
                     /translation="MKLAEFSPFFNCKLKNFLDELHISKPFKDVVSYTPLAGGKRLRA
                     YLIWELSRYTGFGEENALKAGIAVELFHSGSLIHDDLPAIDNDTIRRGLPSSHIKFGE
                     SKAILAGDFLMLFPGRVLSSLDLETRYIHPILELWQETSLKVVEGEFYDVFPEDESRE
                     QMERIHTAKTGALFGFCFAAPFIREKREKIDEMYKLGVRFGKLFQMMDDIKDVISTES
                     ELGKTPGKDGKQNRLTILSFKSLEEAQIEVKESFHSILVEIDSFTDLKKEMKDIYYLI
                     ARR"
     gene            complement(26026..26847)
                     /locus_tag="PKL93_07140"
     CDS             complement(26026..26847)
                     /locus_tag="PKL93_07140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNQ71068.1"
                     /translation="MKKRYRIIEPLGRGGFGMTYLCSDFHTGNSVAVKEFFPRGAERE
                     GNFVKPISSDLKEAYFERLSSFSEEFTIMSKIDDDRVVKVLDLFTENNTAYYVMEFIS
                     GRTLRDTVKSDGVVDESKALEIIDEILKGVSSIHERGYIHGDLKPTNVMLTDEGKIKV
                     MDFGAACLKDVYLMNSLSKVASLSYTCPEKFLSSSTPSYSWDVYSVGGILFFLLSGED
                     PIPSTERIKGIPLVLDLFPRKARRILEKSMSLVADKRYTDADHFRKALRARLFGF"
     gene            26932..27774
                     /locus_tag="PKL93_07145"
     CDS             26932..27774
                     /locus_tag="PKL93_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNQ71069.1"
                     /translation="MFVDFHCHSDHSDGTDSVAAIVQKAADKGISILSVTDHDTLAGQ
                     NEARELCLRAGIKYIRGVEISCDFHETLDVLGYGMVFETSSMEEELRQLRLKRDLRNT
                     RILSRLRSLGVEITDEELESSFPGESLGRPHIANLIVRKGYSDSTENAFKEFLGKDGK
                     AYVEKEKLSIKSAIELILSAGGVPVLAHPLSMMLDRNGTVEIVRTMMRYGLKGMEVFY
                     KTYEEATREELFEISRNLGLIATGGSDYHGKHKKDLELGIDIPDSAVFSFLESLPADN
                     GLEK"
     gene            complement(27928..28824)
                     /locus_tag="PKL93_07150"
     CDS             complement(27928..28824)
                     /locus_tag="PKL93_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNQ71070.1"
                     /translation="MKDFSELVSLAKAGGTRRVVLIGSEDREGIRALKHAFDEGIATP
                     VFVGREAETLEILKEEGLDGEVIPASSPEEACEKGIKLVSSGKADVVMKGLLKTSILL
                     KAVLNKEWGLRSGRVLSHIAAMDVPGVDRVVFVTDGGMIIRPDLQTKVSIIENAVAFL
                     KSIGYEKPKVALIAAVEVVNEDMQETIDAAVISKMSQRGQISDCVIDGPLGMDNALSL
                     AAAQIKKISGPVAGQADLLVVPDIASGNFVGKSAVYLAGGTIAGLILGATAPIVIVSR
                     ADSAASKLASIALASYSIGKSS"
BASE COUNT         7746 a         7129 c         5839 g         8147 t
ORIGIN      
        1 ttattgactt tgaaataagt ctttacctcc tcgccggagt aatatagaga gcctatgtct
       61 ctgtccagcc aaattgaaac ttcaagacct ccttgtggtt cttcaggcac gataatgacc
      121 ttctgaagat cgtaagaaac tataccaaac aaagatatcg ccagaaacaa actgagaaaa
      181 acaagaatta tcttcttcac aatccatccc tcctatcatc agatattaat tagactcgtc
      241 ttcgtcactc tcgttcaaca gtacttcaac atccttactg cttgtattat acttcttgac
      301 aaatgtaatc aatatcatcg aaacaactgt cccgaataat agaccgtaca agcttctcag
      361 cagtcccgtc caaagtggga cggtggcatg acagaaggta ttgacgatcg acactatcac
      421 aagagatcct cccgatgcaa tgactggagc gagttttccc ttaatgttga aagagaagtg
      481 aatcaggatg gctagcaaag gatatgcaag tagctctttg aaacgtggac gaaccagcag
      541 gagattgtct agatagtccc ttaccattct ttcggagtca agaacaaagg aaaagtttcc
      601 acttcttaga atgtagtaac cggccgctat tccggcaaac acaaatacca gcaggtcgcc
      661 cttagatatt tttctggaga cactcttcat aaaaccggtt acgaataacc aaccggggag
      721 ggcaacaaga gatactttca cacccctgaa gagctctaga ccattctgga attcgactcc
      781 gaccatggat gcgttgatag agattcccag aaaaacagaa agcgaaaaga atagtagtaa
      841 ccctctgaaa accccggtat ctagagaaga caaccttctg taaatcgcaa actcccctgc
      901 aattgctgcc agaggcagac cgatagtcgg gaaaagtaga aatacaactg tgattccagc
      961 ggcgccaagc ggactaactg ataggatcaa cacaaatatg aacaacgctg aaagcaattt
     1021 gaatcttgat gagccagtcc tcggagatgt tatttcgtca gagattaagt cctgttcgtt
     1081 tagttcctcg taggatttgt gaactagctc atcatagtcg aaatcaaccg gaggagttaa
     1141 gaaaagcaat gcgtcaacac ttcgctccct aactgatcta atgtacctcg ttacagctct
     1201 atcgatatcg taatttggat actctttctt cttcagattg tggacgcgga aaaccagaga
     1261 cttgttatct cgggccttta agacgtcaag aacatactgc cctctttcgt caaactccat
     1321 gtagccagta taagcgcaat gactatccag aatagaagcg agttccgaaa cagggtacgg
     1381 agaaaccccc gtaagtatca caggaacctg caagtctaga atttcctcca gtctattcca
     1441 gtcatctcta agatccataa agataaccgc agagccaagt tctgaaattt ccgccagatt
     1501 atctacgtaa atcagctgct ttgatggaac gccgtcaacc ttcttctcgg taacaagagc
     1561 tgcacggttg tacttagaat ccagggagac tctccaagga atatatagaa gagacataag
     1621 cagaccaaca acaaaaacaa cgataactat gccgttccca agactgatta cattcttctg
     1681 tttttcgttt cttcgtcttc ttcgaggctt cttaggtcta acgggaactt ccgccaatac
     1741 ttatctctcc tcccgatcag aagataaaga aataatacta caccgacaac aaatggggaa
     1801 tatggaagca aaaaaacaat cctccatttt aaaagcattg aacgaacagt ggatccaaac
     1861 acattcaaaa aagcggtgtc tatttctctt tcagtagaga actcattgac aacttccagt
     1921 agtttgttgt acgaattcct cttgataatg tagtcggcaa agcttgccac tgccccgtag
     1981 aagacacttt gatcatcctt aaccgggtat ctctcctcgt attctgtgaa gtcaaaaaac
     2041 gctactctta aagccgacct cctagtccca gtgtgaaggt tctgaaatcc ctcggcaatc
     2101 acctcatgat accacagcgg aatttcgtca ggattaatat ccctgattct caggtactca
     2161 aagaaataaa catgggcgag ttcatgcctc gcaactcccg gtttatacga agaggtgtaa
     2221 agaaagattt gatatattcc attattgtct attgccgcag gctgctcgtg accttcaccc
     2281 tttaaaatcg taagactgta ttttaccgag aaaccataga tttctgaaag ctctgcattc
     2341 gcttcctcaa tctcacttct gatctccgcc tgacctgcca ccgggagtga agagaaaaca
     2401 accacaataa tgagaaatac aaatcttatt cgaggcaacc cagcaccctc ttaaggtcgc
     2461 cggggagttc agagatgatc tctatttctt ttccgtcatt tctagcgaag gaaagccttg
     2521 aacagtgaag aaaatatcgc ctgagccccg tttctttctt gaagtctcta ttgaacagtc
     2581 tgtctccata cacatcgtct ccgacaactg gataacccgc ttcacttaac tgtcgacgga
     2641 tctggtgctt ctttccagta aatagatcga tccacaacaa agacgtcgta gcatagctct
     2701 tctcaactgt gaaagccagg gattcgacga atttctctct ctgcgaaaca agttttcctc
     2761 tcttttcagg aaatcccttc acaagagtga agtaaccttt ttcgactccc cgttctctga
     2821 atagctttga aagctctcta gcagcctccg gggatttcgc gaagaggatt acgccagaag
     2881 tgtgtttatc aagcctgtga acggggcgag gaacaaaacc tttcgaattc ccgtacgcca
     2941 tcaatccttc aaccagggtg attatctgga ttcccttacc aggatgaacg gagattcctg
     3001 aaggtttgtc aactgcaaca ataagatcat cttcgaacaa aatgtcgagt ggaatgtgct
     3061 taggcgtcag ttcgcgcgat tcttcaagac gcttcagttt cgaaggatct cccgagaaga
     3121 ctacctgaac cttatcgcct atttccattt tccatgagcc gtctctgatt ttattcccat
     3181 tgaccctaac attgcccctt ctcagcaatt tgtatattga ggagagcttc aaatcggaaa
     3241 gctctttcct caaaacccga tccaatctct cgtaaaaatt ttcttttgtt actttgaact
     3301 ccttttcgat cgaaatcact ccactcaaat taaagggggc attaagcccc cttagaaaat
     3361 tcaatcctca acgcctagac ctttgccgat actctccaca atatccccaa ctgtggagat
     3421 gttctcgatc tgctcgtctt caaccttgaa atcaaattcg tcctcaagtg ccattaccag
     3481 gtcgaccaaa tcgagtgaat ctgctccgag atcctcggtc aaatctgaat ccatggcgat
     3541 ttcatcttcc tcgactccaa gcttctcaga aattattgtt tttactctgt cgaatacttc
     3601 atcaactgtc aaaggacatc ctcccttcga gatctttctc aaattgcaat aattatagtg
     3661 ataccaggct ccaaagtcaa attctggatt cctagaagct ttcattatta tataataatt
     3721 tgaaggtctt tacttctgga ggtttgctat gagactctat acagagatga aagaaacagt
     3781 tgtcaggcgc ctaacgactt ttccgaaagt tgcgatgata cttgggtcgg gactgggtta
     3841 ccttactgag caatttgaag aacccttggc aatagattac agagaaattc cgggatttcc
     3901 cacagctacc gttgaaggtc attcgggcag gctagtcttc gggacattcc aaggactccc
     3961 ggtggtggca atggaaggac gttttcactt ctacgaaggt catagaattg aagacgtttc
     4021 ctcaccaata tatctcttta aagaacttgg cgtaagcaac ttgctcatta caaatgcttc
     4081 tggcggaata aacagaagct tctctcccgg agatatagtt gcagtgacag atatcataaa
     4141 ctttggattt cgcaaccctc tgaggggaga aaatgaccgc cgttacggcc cgagatttcc
     4201 cgacatgtcg gagataattg attttgcctg gctaaaagtt ttgaaatcaa ggctcgagaa
     4261 agcaaagata gatttgaagg aaggaaccta ctgctggact ctgggaccga gttatgaaac
     4321 gcctgcagag ataagggctt ttgaattctt cggggcagat ctcgtgggga tgtcgacggt
     4381 ccccgaagta atcgccgcaa gacattgtgg cataaagctt ctggttttat cgtgcgtgac
     4441 aaatatggca tctgggatcc tcaaggagaa gctcactcat gccgatgtgg tgaaaacggc
     4501 aaacagaata agacccaaat tcactgcagt tgtccagcac gcggttgagt cgattaagga
     4561 gactgaacaa tgataaggaa gatcaccagc attgtatcat gcatacagaa ggccaacgac
     4621 attctcgttg tgggtcatat aatgccagat ggtgattgta tctcgtcagt ggtgtcactt
     4681 tcgatgggac tcgagaagtt tggcaagaag gttacaccgg caattgactg gaagattccg
     4741 gcgaatttta cagtctttcc ctggatagat agaattaagg atttcagtaa tgatgttgtg
     4801 gagcccaatc taataatcat tgtagacgct tcatctccag atagaatcgg tagatttgaa
     4861 cagcttttgc gaaaaggaat cccttcagtg gttattgatc accacgcaac caacacctat
     4921 tttgcagacg actgttgggt agatccatca tattcttcgg ctgctcaaat ggttcttgac
     4981 cttctaaaac tcatggatgt tgaatatgac gccgatctcg cactgatgaa ctacataggc
     5041 attgcaacag atactggttt cttcagatat tcgaatgtag atagctcggt tttcgaggcc
     5101 gctgcggagc ttgtgagatt aggagcagat cccaccttcg tggcaacgtc aatccttgaa
     5161 acaagaaaga tcgaagaact tttccttgag agggatgcga tcgacaatat aaagatgcta
     5221 tcaaacggca ggtttgcata ttcctacctt gagttggagg attttgaaaa gtactcactc
     5281 acagaagatg attttgtggg tttcgtcggc gatctgagat cgataaagag cgttgaagtt
     5341 gctctctttg cctcagaagc gagtaaagga gaagctcatg tctcgctgag atcgaagagc
     5401 tacttcgacg ttagcaaaat cgccgttgcg tttggggggg gaggacatca aaaggcctcc
     5461 ggtttcactc tgagatacga cggcaatcta gaggatgctc ttgccgaaac gattgaaatg
     5521 atagattctc aacttcaaca gaaagaaaaa cattaggcta ttaatctagc cagaagctgt
     5581 atgaactggg ttataggcca cataataagc tggattatcc ccatgagtga cagcacgaga
     5641 agtgtcagta ttccatatag ttcatatttg acaagccagt tcacttggct ctccggaaca
     5701 agcattgtaa caatgtttgc cccgtcaaga gggggtatag gaatcaagtt gaaaagcgca
     5761 gtgttaaggt ttatcaccat tgtccagtaa acaacgtctg ctaaataact tgcgggaaaa
     5821 gagctgaccg tttctggcat gaaggtggag aacctgtgaa gaccggagag atagaagatc
     5881 agacctgtga taactccaag cgagaagttc gcaatagatc cggaaagtgc cgtcagtatt
     5941 gccttgaaat acttggcttt tctgagcttc cagtagttga cgggaaacgg tctcgaccag
     6001 ccgaacttga agaaatagaa cattaataaa ccgacagggt caattcttct gatgaagccg
     6061 ggtcctcccc attccggctt ggtggcttcg aaacgtctcg cggttataaa gcgggcatac
     6121 tcatgggaga gtattgccgc agcaatggcg ggagttagac aaaagaaatt cctcatcatc
     6181 tcaaacatct tcctcatccc ttcctgatag aacgaccgtt atctctccct tcacttcgga
     6241 aaacgcgaac ttctggatag cgtcagaaac gcatccgaaa aaagactcct ggaagagctt
     6301 ggtcagctct cttccgatga atatctctct gtcgccaata atttcaagga tttcgcgcaa
     6361 tgtctccatt agtctgaatg gtgactcgaa gaatacaatg aggctttcgc cgtaactacc
     6421 gcgagaaatc ttcctcaaca gtcttctccg attctttcct ctaggcagaa agcctagaaa
     6481 tgtgaaatga cttcctggaa aaccgctgat agcaattgcc gatgtaacgg cacttggacc
     6541 tggtgaaacg tcaacttcaa aacctctatc acggcaaatc ctgatgattt tggcccctgg
     6601 atcagagatg acgggcattc ctgcgtcact ggaaagtgcg atttttgctc cttcgtcaag
     6661 ctttcgaatt atgaaagaca gtctttcttc ctggttatgc atattcaggg ataccacatc
     6721 tttgttttct atccctagag aggagagaag tgatctcatt ctcctcgtat cttctgcaag
     6781 gatcaaatcg gcttcctcaa gcactctctt ccctctaatc gtcatgtcat cgagatttcc
     6841 aatgggagta cctacgatcc agagtttccc ctttttctca gtcactttcg acctccaaga
     6901 ggaagcttcg acgcaactcc tgatcttctt ggaaactcac tcggagtagc tctttccttc
     6961 tcgtaaacta ccataaacct cttcgaaccc tcagaaagtc tgtactcttt ttcatacgag
     7021 agccgtaatc caagaactct ttctgctgca tcagaaaaca ccttctcatc aagccacccg
     7081 ggacccttgt aaaggtagat gtatccccca accttaacta gaggagccgc gagttccagc
     7141 gaaatatcgc atctgccaac tgccctcaag aaggctgaat cgaaagtctc tcctgcttct
     7201 tttgcgaatt cttctgctct tgctgtcttt acggcaacat ttgtcaggtc taaatcccgg
     7261 gcaaatcttt cgatctccct ggttttcttt gcgattgatt caagaagtgt ccagtttgac
     7321 ttaggaaaag aaatcgccaa aacaagacct gggatcccac ctccacttcc aatgtctatg
     7381 cagcggcccg atagagaaga accttcaaac ggaataatgg tgtcttcgat gtgaactgtc
     7441 attgctcttt caaattcctt aattgagctt agattgtggg gagaagagag catgagctct
     7501 atgagttttt catacttcgc gaaaaacgac ggctcaagaa attcactcga gaaaaccatt
     7561 ttttcattcc ttttctatac atgcgtatgc ttcgtgattg tgaattgact cctgactgat
     7621 agcttcgact ctgtaccagt tgatccgact atcatcttga agaagtaaga cgacttctcg
     7681 cgaaagatct tccacaaatc tggggttgtc gaaagagtgt tcggtgatga acttctcgtc
     7741 ttctcttttg agcaaagaaa agattggagc acttgcggat ttctcgacat actcgatgat
     7801 ctcttcaatc caaacaagcg aattcattct tacggaaaca aaaacctctg ccctttgatt
     7861 gtgagcacct ctgtcactaa tctcttttga gcaaggacag acggtcatga ccggaacctt
     7921 gactccaagg acaaaatcaa acgttccatc tttcattgca atgaaggaac acttgaatga
     7981 gctgaaactc tcactcccgc ttattggagc cttctttctt ataaagtaag gaaactcaac
     8041 cttgatatga gccacatcgg ctttcaagga gtctctcatg tcattcaaga ttgactccat
     8101 gcttctggga gtgattttct tatggtgtct ctcaaggacc tctacgaatc ttgacatgtg
     8161 agtaccacga aaatccttag gaagatccac aaacagatcg aatttcccca cagtattctg
     8221 tgttccgaat tttctgtcta atactactat gggatactca attgacttta tcccgaccat
     8281 gttgattttg atgtttctct tgtccttctc attctgaacg tctctcaaga tcaaacaccc
     8341 cttgaatctg tggtctcaaa gaggtataat cagctaagtg gaggcgtatc ctaactggct
     8401 aaggagccgg tcttgaaaat cggtggggtt gacgcccctt gtgggttcga gtcccaccgc
     8461 ctccgccaaa accgggggtt tccccggtta tttctttagc gggccgaaat cggaaaaagg
     8521 ccatatagtc aacatcggac taccgattat tgcctcctct ggaacgaatc caaaatacct
     8581 gctatcgaaa ctattcgctg agttatcgcc catcataaat cggaaccctt ccggcacggt
     8641 aactctgacc actcccgttg acggatcctg agtgatgtac ttctccagac cgaggggctc
     8701 taactctgtt tcgaagaact cagtatattc gatcagcccc ttataggcct gataaagcct
     8761 gtaataaggg gagaatctca ccgaaggatc gtcggggcga gccaagccta aatagaagct
     8821 gggatcgtaa aacactccct ctctaacata acgcctttct tcaagtgcag gtggaatctc
     8881 tccatttacg tagagctgat atacgggatt cgccgcagta taatcaggcg ctcttctgag
     8941 ttcaagaacg tctccagcct tccccaccaa tcgctttaca tatttgacat gtcccctgta
     9001 cttggctgga gaaaaaagat ccatgaattt atcaaaccct ctgagatact tctgagactc
     9061 aatatccaca taaggcgtcc agaacaccac tatgtctccg taatcaggct ctctgtattc
     9121 gtaagtgatc ttctccacaa acaatctggc gggaggattt atggttggta tcattgaacc
     9181 tgtcggaacc agcattgtct caaaaacaaa caaacgaata atcgtaccaa atatgacagc
     9241 ataaagaatc gcctttcccc actccttaac ttcgtggaga aagcgcgatt ccttcttgtc
     9301 agaacccttt ttcgactttt tggcagacac ttagcctcag tcccttttct cttttatctt
     9361 taccttaccc ttcacgtctc taatgtagta gatcttcgct cttctgacct ttccccttct
     9421 aagaacttca atcttctcga gagaagggct tgtgaacggg aatatccttt ccacaccaac
     9481 acccgcagct cctactcttc tcactgtaaa ggacttgccc gtcccgctcc ctcttattcc
     9541 cattacaatt ccctcaaagg cctgaattct ttctttgttg ccctctttga ttctaacgct
     9601 tactctgacg gtatcgcccg ggcccatctt tggtaggtcg tccctcaaat gctcattttc
     9661 cagagatctt atgtactgat ccatctttat ccctcctaac ttctactgta tcttcatgaa
     9721 ttaatctatc taaaactatt gcaactgctg ctctcacaga gaggtgatta aaatccgaat
     9781 tcgctctaag cggttcaaga gcatagtcgc aaatcttctc aatttcaccg ggtaagcccc
     9841 agcttgttcc aaagaggaga agatggggtt tttcactttt aacgattatc cttcgcatgg
     9901 catcgaaagt gattctgtct tttcccctct ttgccgaagt aaaaacgagg tcgggcctct
     9961 gtccttcgat ttcttctatt tgctctagaa catcttcaat gtatgatgcc ctctcaacta
    10021 ctttaagcgc ttcacttctg ctggggttgt attcccttcc aaactcctca agccagtaat
    10081 tcagaacatt atctactatc tctctctgcg ccgggagatt acttaccacg aagtaaccct
    10141 tgacattgta tgtcctgcat gttcgggaaa tatcgtgaac atccagattt gtgactgcgc
    10201 tggaaacgat atttccgtgt ctgcccaaaa cagggtaatg aatcaaagcg agatatatgt
    10261 tattcagcat cctttttcag ctccttgaag agcaacaaca gtgctttctt gtcagtcagg
    10321 tcaaaatcat gcttgagaaa gatatccggc cttctttcga tagttctgag caaactctcc
    10381 attcttctat gaatcgctat catttcatgg ttcccactga gcaatacttc gggcacttcc
    10441 atgccgttta cttctctggg cctcgtataa tgagcataat caagaagatc ggaaaagaaa
    10501 gaatcgttaa ttaccgactc catatcaccg acaactcctg gaacaaacct tgaaatgacc
    10561 tctatcatca acagtgccgg gatttctcca ccgctgagca cgaaatcacc gatcgaaatc
    10621 tcctcgtcaa tggaactcat tgccctttca tctattccct cataacgccc gcaaacaaaa
    10681 acaatgtggc taagcttact cagttcaaga gctttcttgt tgtcaaagag cttgccctgc
    10741 ggagacggat agacaacgtg gggcttgctg tcatccgaaa agcgacttgc aactgctttg
    10801 attattggct cggccttcat tacgaggccg ctcccccctc cgaatgggta gtcatcggtt
    10861 gttctgtgac gatcatcggt gaagtctctt atatcgaccg attcaaaagc aattatgcca
    10921 tttttaatag cccttgatat taccccccaa ttgaagaccg tttcaaacag ctggggaaag
    10981 atcgtcaata cctctatctt cattcgggct tgacctcttc gaattccata gatctggcaa
    11041 caatcgtctt atcttcaaaa ttgagctcga gaatgtagtc cctaattatt ggaataagct
    11101 cttcacgaac agtcaagtcc tcttcacgaa cgaccaccat aacatcattt gctccagttt
    11161 cgatgatgtc atttacagtt cctatgaaac gccggttctc atcgaatacg ctactcccaa
    11221 gaatctgaaa gtaataatac tctccctgtt tcaattcagg caggacacgt tcttcaacgt
    11281 atatatggaa gcccacaagc ctttctgcct gacccataga gtcaattccc tcgaaatgca
    11341 agatccaacc tttggtggct ttccgggccc catcgattga agttacaaac cgcgaacggg
    11401 tcttttcatc ataaagcaga acctcctgga tctctttgaa agctgtttca tcttcaagag
    11461 cgacttttac cttcatctcg ccacgaagtc cgtgaggctt cactattctc ccgattggaa
    11521 ccatttcctc caaagacttg tctttcagtt cgctcatctt accaccttta ggataaaact
    11581 ctcctttgat gatccggcga gtgcgttgag gagaacattg attgacttga tagttctacc
    11641 gtcttttcca attatttggc cgacatcttc ctctgcagcg ctgatttcaa agaccatgtt
    11701 tccgttttcg tctactgctt cagtaacagt tatccgatcg ggctccctca ctattccctt
    11761 cagaatatgt tctagcactt ccttcattac tctgctttct ctcccgattc cttgacatac
    11821 ttcagctcgt ggaccttctt cataactccg aaccttgcaa gaatcgatct tgcggtctca
    11881 gtcggctgag cacctttcat tatccagctc actgccttgt ctacatcgac actcataatg
    11941 ccgggatctc ttatcggatc atagaatccc agagagtcaa tataggcccc gtctctacgc
    12001 ttttgtgaat ccactacgac aagcctgaaa aacggcctgt tccttctacc catccttgta
    12061 agcctaatct ttaccattct tttcgtgcac ctcctgattc tctactatct aaaatggaaa
    12121 tcctttcata cctttaagta gtttcccttt tcttccgaac tgcttcatca tactcctcat
    12181 ctgatcgtac gacttaagga gtttgttgac atcctgaatc gttgttccgc tacctctcgc
    12241 taccctttgc tttctagaat agttcagaag ctttggattc cttctctctt tagatgtcat
    12301 ggagtggatg atcgcctctg ttctcttcat gctcttttcg cttcctgcaa gatcaacgtc
    12361 tttaggagcg ccaggtatca tctccatgat gtctgcaatc gaccccagct tttttacttc
    12421 tttcagctga tcaaggaagt cctcaagatc aaatttgttc ttgagaaatt tctcttccat
    12481 ttctttggcc ttttcttcgt caatgttata ctgcagctta tcgatgagcg acaaaacatc
    12541 tcccattccc agtattcttc ctgcaactct gtctggatga aaggcctcaa taccgtcggg
    12601 cttttcagag attccaatga acttgacggg ttttcccgtt acatgcctta ttgaaagaat
    12661 tactccgcct ctcgcgtcgc catcgagttt tgtaacaaca aacccggaaa gctcgagcgc
    12721 ttcgttaaat gccttcgcag agttcacggc gtcctgtcca gtcatggcat cgacaaccat
    12781 tagtatctca tcgggcttaa cagcggaaac gatttccttc aactctctca tcatcgcatc
    12841 gtctatgtga agtctacccg cggtgtctag aatcaccaca tcatttatgt tcctaaccgc
    12901 ctgctgcatt gcttcctgaa ctatctttac gggattcttt ctgtcgcccg tgaaaacagg
    12961 catatcaatc tgttttccaa gttgcatcag ctgatctatt gccgctggtc tgtacacgtc
    13021 cgcagctacg agaagaggtt ttcggccttc ctttctcaac aatgtaccca gcttggccgt
    13081 agtagtcgtc ttcccacttc cctgaagacc caccatcatt atcttcgaag gctggctaga
    13141 aagtttcaat ggaaccgact tctcgccaag catcttgatc agttcgtctc tgacaatctt
    13201 tataaactgc tgatcaggcg tgaacgattt cagtacctct tctccaagcg ccttctcagt
    13261 gatgccgcct ataaactcct tgacaacctt atagttgacg tccgcctcca gaagcgacaa
    13321 cttgacctgc tttacagcat cttctatatt cttctcagag attcttccct ttccacttag
    13381 cagcttgaaa gctctgccca gcttttcctg gagattctca aacatctgaa cacctccgca
    13441 gcttgaaaag tatacaacgg gccagcgttt gtatgttgaa cttatctgaa caacaacagc
    13501 gattatcggg caagtatatt aatttttagt taccgtgaag gtatcaatat acttaattaa
    13561 tctatgaatc ttcgaagaaa gtgtttctaa agattattgt cagtcatcca tcttagcgtc
    13621 ggagaattct ataaaaaaga ccaaaagagg aaaaatagct tcttagcccc agtctttcgc
    13681 attcagtttt ttgtaacaat ctctacaaac cgcgatgtat ttttccatac cacctatatc
    13741 tatttcgctg tcagttggac ctgaaattct gtgagtaacg gtggcattgt actcaccgca
    13801 tatgtgacaa acagccttct tctttatcac ctcgtctgcc gcagccatca gattcgcggt
    13861 tacggcaaaa ggcctgtgct tgtaacttaa atcgagacca acacagaata catctacacc
    13921 actgaagatt atctcttcta caatgcgaag gaggttttcg tcaaaaaagt tgatttcatc
    13981 aataaaaatc gcgtcgagtc tccccttttc cttggacacg atttccttga tttcagagga
    14041 atcgtaaaca tttatcgctt ccgccatctg accggagtga cttacaacat gatcagaaga
    14101 gtatctgtcg tctattactg gtttaaaaac ctttattcgc ttttttccaa gtgtgtagat
    14161 ttcaatcatg gaaagaagcg tcgaggtctt tccagaatac attggtccca ctacgaccgt
    14221 gagcttaccg gacatatcct caacccccta tcacgtaatt aagactgccc cttatcccaa
    14281 gcataaactg ctcgaccgac ctggtctgtg agcaaaactc tccgcttacc tttactgctt
    14341 ctccaatagc ttcttctgga gatgctccat tcatgagaag cagcgaaaaa accgatccaa
    14401 atatatcccc acttccgaaa cgattcttcc caaccacggg tggcaaataa tccttgccag
    14461 caaagttaac gcccttttct gaagccttta taatcaggcg gctgtcgctt ttttcaaatg
    14521 acatgtagtc atttgccaga ataaaatcat acttccccag tccatcgaga cctttgaact
    14581 catgaaaggg agatggatca acaactttga tcctcgactt caccgaacta ataactgatg
    14641 ccgcttgctg accaatttcc agagtactgt agagcagatc gtactcttca tcgatcagaa
    14701 ttcctaccag ctcttgattt acgtcgacag gctgaggttt ctgcacggca atgacatcag
    14761 aaccgctgaa gagaaaaata ccgtgtgcac aatcaggcat aacggggatc gatctgaaag
    14821 accaccgatc agaatcgcaa acaccaaaac aactcaaaaa tagcacttca gcaccgagta
    14881 acgaaagact ggttgccaca ttgaatgccg atcctccatc ggtcttgact atactggctt
    14941 tgtgaccgct gcccgatact tcaacatcct ctaggtaacc tccaagaacc agggccctaa
    15001 acttacttcg ttctgtaaag tctgtcaccg gcatctccta gacccggaat tatataggca
    15061 tggtcgttga gtctttcgtc tagagccgcc gtaaagatct tcacgtcggg atgattcttc
    15121 tcaatgaatc ttacaccctc cggagcagcg atgagacaca ttagcgagat ttgtttcgcc
    15181 ccggctttct tcactacttt cagagcccac gatgaggaca ctcccgttgc cagcataggg
    15241 tctagaacga acagctgagt attttcatca acactcggaa gctttgaata gtactccacc
    15301 ggctgtattg tgtcgggatc tctatatatt ccgatgtatc cgaccgaagc gttgggcatc
    15361 aatgaaagaa ctccttccac cattcccagg ccggccctga ggatgggaac gaccgtcact
    15421 ttcttatcct ctatacactg gcccaccatc ttcaccagcg gtgtcttgat ttccttatcg
    15481 tacgtcggta tatgtctcgt cgcctcgtaa gtaaggagta atgtgatttc ttttaagagc
    15541 tccctgaatt cctttggacc ggtctgatca tctctcatta ttccaagctt atgttgaata
    15601 agcggatggt tgactaccgt aagctggtca aacacctgca tcactcctgt gaatcagatc
    15661 tgaataaagt gggaatttgc ttgttagcgt cttgacttcc tcactgatct ctctcacttt
    15721 tgtctgtgag atctctccct tttctccctc tatgttctct agaactcgaa taattaaatc
    15781 ggcaatcaag ggcatttctt tctcagtcat gccccttgta gtaatcgcag gacttccgat
    15841 ccttattcca ctggtgacaa aaggtgacct ggtttctttt gggatagtat ttttgttgac
    15901 cgtaatgtcc gctttttcca gggccttttc tgcagctttt ccagtaacat tcataggatt
    15961 cagatcaacg aggaacagat gtgtgtctgt accgccagat actatcctaa gacctttttc
    16021 ctcgagcgac ttcgccagtc tgcgagtgtt gtagattatg ttctgctgat aggccttgaa
    16081 ttcgtctctt agagcctcac caaacgagac tgcttttgat gctatcacat gcatgagcgg
    16141 gccaccttgg gtacccggaa acaccatctt gtcaaccgac tttgcgatct cctcgttatt
    16201 tgtgagaatc attccccctc gtggccctct tagggtcttg tgcgtcgtag tcgtcacaac
    16261 atgagcaaaa tcaagggggt taggatagag ccctgctgcc accagcccag caaagtgggc
    16321 catatctact atcagaacgg cgtgtacctc gtcggcaata tctctgaatt ttttgaaatc
    16381 gattattcgg gaataagcgc ttccgccggc aacaattaca gaaggtttag catcaagagc
    16441 aactttcctc acttcatcat agtcgattac ctcggtttcc tcattgactc cataagagac
    16501 tgcattgaag agtttcccgg agaaattgac cggagatcca tgagttagat ggcccccgtg
    16561 gctcaaagac atacccataa tcgtatcgcc tggcttggca actgccagat aagcagccat
    16621 gtttgcctga gaacccgaat ggggctggac gttgacaaac cctgcatcaa aaagtttttt
    16681 ggctctctca cgcgccagat cttcaacttc gtcaacgaag acacatcctc cgtaatacct
    16741 tctggaagga taaccctctg cgtatttgtt cgtcatgacc gatcccattg cttccattac
    16801 agccctggaa acaaaattct ctgaagcgat caactcaagc ccgtttctct gtctttcaag
    16861 ctctttgaac attatgtcga agacttgttt atcagttttc tctagagatt cccacattca
    16921 gataacctcc tccggattct cttcaaacat ctctctcagt gatacgtcat atggcggcct
    16981 cgcaattccc ttttccgtga ttattcctgt tatcaaactc gatggggtta catcaaatgc
    17041 tggattcaaa cacctcacgc cttccggcgc aatttgttga ttgcctatgt gagttatctc
    17101 tctatggctc ctctcttcta tcggtatttc ttctccagaa gcggttgtga gatccacagt
    17161 gctcagtggg gcagctatgt agaaaggaat cccatgcctc tgggcaagta ctgcaacgga
    17221 atacgtccca attttattcg cggcatcgcc attagctgca attctatctg ctccgactag
    17281 aaccaggtca atgaatccct tcttcattac ccatcctgcc atgttgtcac atattagtgt
    17341 cacgtcgatc cctgatttaa tgagttccca ggccgtcaac cgcgctccct gaagataagg
    17401 gcgagtttca tcggcgaaga ttttgattct cttaccctgt tcgacagcag ctcgcattac
    17461 cccgagagcc gtgccgtaat cgactgttgc aagagctccg gcattacagt gagttagaat
    17521 tccatctcca tctctcacaa ccgttgctcc atttcttcct aacgatttat tgatttctat
    17581 atcctctttt gcaatactca gggccgctcg ctcgagcgag tcgatgagtt tcttcctttc
    17641 caaaccccag ttagaattaa gagcgttttc cattctgtcc agggcccaaa acaggttcac
    17701 ggcagtgggc ctactctcgg aaagcctttt cttaacagtt gtcatgtgat ccaaaaagac
    17761 ttcatcggaa gcatccagta cttcctttgc acctagcaga taaccgaagg ctgcagtggc
    17821 gcctattgct ggagcgcctc tgactatcat ttctcttatc gcaacatata cttcttcatg
    17881 gttcctgcat tctacatact cttctgccgt aggaagcttc ctctgatcaa tcaatctcag
    17941 gcggtcttcg ctccactcaa gtgtcaaact tttgaaatga ctcactaaac tttcaccctt
    18001 ttcactatct ccctcactgt ttcgcggtaa gcgttttcca gctcagatcc tctcatgtta
    18061 gtcacaatgc ttttcccggt gtctgaaaaa tctacgagtg cgggatcaaa tggaagagaa
    18121 ccgatgagcg gaatgttgaa gagattttca agatcctttc ctccgccctc gccaaagagt
    18181 cttatcactt ctccgcagtg tgggcacctc aaataagaca tattctcgac aattccaaga
    18241 atatctctgt gcatggtctt gacaaaattt atcgccctct tcacgtcctg aacggcaact
    18301 gcctggggag tcgtgactac aacggccaca agatcggtta gagtctgcag aacggtgagc
    18361 ggctcgtctc cagtccctgg aggagaatcg attacgagaa agtctagttc accccacata
    18421 acatccccga gaaactggta gatcgctgta gtcttcatcg gcccacgcca aatcaccgga
    18481 gcatcctctt caacgaaact tgagatgcta ataaccttca ggttaggcag agcctctgga
    18541 gggaaaatct gtccttctac aacttcgggc tgctttttca gaccgaccat cctcgttacg
    18601 ttgggtccat ggagatctat atccatcaga ccggtcttga agccttcatc tgccaaagcc
    18661 agcgctagat tgaccgcgac cgtcgtcttt ccaactcccc cctttccaga cattacgagt
    18721 atcttattct ttattgactt aacgttgtct cttatggttt ccattctctc tgccatttct
    18781 tttggattca cttttcttca ccttcatatt caattctcat tgacatatcg gccgttttgc
    18841 caagcatagc cgagacagaa cagtatcttt cctgagagag ggtaaccgcc ttttctatct
    18901 tatccttcgg tagattcttg cccgagaagt gatatacgag ttcgatgctc gtgaagactt
    18961 tcgggtgttc ctttgcatac tcataatttg cagaaatcca gtactcgtag tttgtaacct
    19021 tcatcttgga gagaatcgaa actacatcca ttgaagtgca acccatcagc gcggccatta
    19081 ccatctccat tggagctggc gccgatccat cgcctccaga actttcattt gcatccatat
    19141 gtatatcatg gccagaagga gtctttgcat agaaggccat ctttcctatt ctccttgcag
    19201 agtattccat cgtccttcat ctcccttcaa aaaaaaaggg cctgagcccc ttttaatttc
    19261 cattcaacgc cgatttggcg atttcacaat actctttaaa cgttccaaaa tcgttgactg
    19321 caagatcggc aagcatcttt ctgtttatgt ttactcctgc aatcttcaag ccatgaatca
    19381 gatcgttgta cttagttcct gcgattcttg cgccagcatt tattctagta atccagagct
    19441 tcctgaaatc tctctttctt actttccttc ctgcgtacgc gtacactccg gatcttatgt
    19501 agtactgctt tgcaagtctg taccttctac tgagagcacc tctgtatcct ttagcggcct
    19561 tcaaatatct cagctttttc ttcttagtgt ttactccacc ttttacacgc atctaaatct
    19621 cacctcacaa cagggtcaaa tgcccaggct tttcttaact ttcttctcat atccgccctc
    19681 gaccagtttc cactttctgg cctcacgacg gctgctttcg ctcttcttac cggtattatg
    19741 gccagttcca gactgatttc tcatgatctt tccactaccg gtcaccttat accttttggc
    19801 acttgcctta tgagtcttca tttttacttt gttgcccatt atctttacct ccttgaggag
    19861 ttgtttttgg cttaaggatc atccacatat ctctcccctc cagctttgga ggccgatcga
    19921 tatcggaaat ctgctgaagc tgatctgcaa ctcgttcgag aatctctctc cctttatctg
    19981 aaaagacaat ttcccttcct ctgaacatga ttactactct tactttgctt cccttttcaa
    20041 gaaactcttt gatcctgttc gtcttggtct ggaaatcgtg ttcatcgatt ctaatcctga
    20101 acttcatctc tttaagctgg gactgcttag ttttcttctt cgcttccttc ttgcgcttgt
    20161 ctttttcgta cttgtacttc ccgtaatcca tgatccttgc aactggcgga ttcgccgcag
    20221 gtgctacaag aacaagatcc agtccctttt ctctggcaat tctcaaagct tctgacgtct
    20281 ggactacccc aagctgttcg ccgttcatgt caacaagtct aaccgtcttc gtcattattt
    20341 cgttgttctt cggagttgag tcacctcttg cgatactaca tcacctctca aaaaaaattg
    20401 gcggaaaatc ccgccatgtc aataaccctt ctttcaggta ttgacccctc aaccttaggg
    20461 cgggggtggg agaacttctc cacttaatct tctcactctg gtgagccgtg cagggatcga
    20521 acctgcaacc tattgattaa gaatcaatcg ctctgcctgc tgagctaacg gcccactaac
    20581 ggtatttatt ctatctcatg cgaatgaacc tgtcaaccgg gtttcaacgg taaagatcgt
    20641 ctctaaagtt ggagaaataa ccgtgtgaat cacggctctc tccgaatcga tacttcttga
    20701 ccagcctaat gtcgatctcg gcagaagcaa aaccttcggc tcctccgcaa tctacaataa
    20761 tctcgcctga aggtgagatt attctgcttc ctccaacaaa gttcattgtc ttatttgtac
    20821 cgcactggct agaggcgacc aggaagcatc cattctctat tgagcgggct ctggttgctg
    20881 tctcaaagat caatttcctt tcccttccaa aggcaaatgg aaccaggaga acctctgccc
    20941 cctgtttaca tagctttctt gagatctctg gaaaccctat ttcgtaacac ataagcactc
    21001 cgaactggat tccagagaac tcgaaagtct tgaattcact accgggagtg aagactttct
    21061 tttcatcgcg gaaaaggtgg gttttgttgt agtaaatcgg ttctagataa ggcaacatta
    21121 tgacagtaga gttgaaaaaa cctccgttcg ttttgtttac gattccacct acaactgcaa
    21181 tatcattgta gtctgcaaga gcagagagaa cctccacggt ctcttcttgc atttcagagg
    21241 cattgcttat agtcgcctca tctgtgccgt acccgctgtt gaacatctcg gggagaagaa
    21301 caagatctac attttcttca acgagactct ctatgtaact ggtaatcttc tccagattct
    21361 ccccaggggc atcattatcg gctctgaatt gaacaatccc aattttgatc tttcccgtgt
    21421 tacccatttc agctttcacc atcctgttca aagggacttc taactaatgg cttgtagatt
    21481 ctcctgatgt cttgaaacgc aacaagagcg atcaatgcaa taccaaggat caaaccgccg
    21541 cttccaagtc gcatccatgc aattctgctt aaggcagtcc caaccagaat ccagtatccc
    21601 agggaaagga cgaccgatag agatcttctg tggtgaaaaa gcccatagaa ccccgagact
    21661 ccctgtgcta ctgcaaggat tacaacttcg cttctgtaaa ccatgcctgc cgtgtcttga
    21721 aaatagccaa atagaattac aacatagaac aaagtaagcg tcaaattctg aatcctggca
    21781 acccgctcag ttttgctacc gaggatcact ccgatcagga ccagggaaac cacaatcgcc
    21841 gagagcataa tagaatagtt caggaatggc atgataaaga gtagaaacag agaaaaaatt
    21901 atcctgtata tcatcagtaa ccgttatctt ccagccactt ctgagcgttt tcttttgctt
    21961 cctgaccttg aacgataaaa tcgggctgga gttcgagttc aagtaccttt ctgtaatact
    22021 gggccgccag atccttcttc ttccaaacct gataaagcac cgccaactca taatagctgt
    22081 tgagatatcc cggatcgttt tcgatagcct tcatgaaccg ctcttccgac ttcccgtaat
    22141 ttctgtacgg ccagggtgta tcacgatccc tcatgccctt agcaatgaat gggaatgggc
    22201 tatcaggcat cagttccatt gctttgtcaa tactcttgtc gaaatccgat atgagaaaca
    22261 tggactgaat tattcctgcc atctgagcga gttgtcctat tgccgctccc ttaacatagt
    22321 aggccattcc atatgagtca tccattttta ttgcctgttc tgcaagctct cttgccttgt
    22381 tataatgagt ctctcttgtc ttcttatcct ctatccagtt cgcgtattcc cggtgacaat
    22441 ccgcgagaag agtgaggatt tctatatctt ccaggtcctc agactcaagt ttttcaatga
    22501 actcggccat catttcggtg tcttccatgg cccttatttg aacaaactcg gaaagcaacg
    22561 cttcgtattc agaagcaaag aaaaccgatg aaaagacaat caaaaaaatg atcacaagca
    22621 ttttcttcat aaaacagcac acctccaaac gaagattgta tgataggaat attatataac
    22681 tacatgaggg aaagattgac ggaggtaggt taatgaatag atttgttgtg gtcaggtatg
    22741 gagaaatcgc tctgaagaaa gggaacagaa agcttttcga gaaagtcctc gtaaacaaca
    22801 taaagaggca actgggaaga tcgacagtcg aaagaatcag aggacggata atcgtctctg
    22861 ttccctcaga gagactaaga tcgtcgatag aaataatgtc cagaacgttt ggcatacaga
    22921 acttcagtgt cggaacatgg acttcctatg atctggaaga tatttacctg acctcggtcg
    22981 atcttgcaaa ggatgaaatg aagagaggaa gaaagacttt caaggttgaa acccggagac
    23041 tggacaagag atttcctcta aaaagcgttg agctgaatcc cctcgttggg gagagaattc
    23101 tcgaagaact ccctgaaacg agtgtagata ttcacaatcc tgaattcaag atagagattg
    23161 agataaggga tgaaggagtg ctgatctcga gtgggaagac aacagcaagc ggaggtcttc
    23221 ccgttggagt tagcggtagg gctttgttgc tgctttcggg cggaatagat agccctgtag
    23281 cagggtggct ggctcaaaag agggggttgg atctcgatgc aatacacttc tccagtcctc
    23341 cttacaccgg agaaagatca tttgacaaag ttctgtcact agcgaaaact ctctcaatct
    23401 ataacggtgg aagagagttc aggctatatt ccatgcattt taccgacgct cagattgcag
    23461 tccataaaca cgttgaagaa agatatagcc tggtctgtca gagaagattg atgatgaaaa
    23521 tcgctagtaa gatagcagaa gctcaaagga taattgcaat cgttacggga gagaacctgg
    23581 gccaggttgc aagtcagacc cttgaaaact tgagagttat cgaagaacag actgagctaa
    23641 tagtgctcag gccgctacta tcttacgata aaatcgagac aatagagctc gcgaaacaaa
    23701 taggcacgtt tgaaacgtcg attttgccat acgaagattg ttgtactgtt tttgttccgt
    23761 cgaatccagt aacaaaggcg aggcttttcg acataaatcg ggtcgaagcc ggactagacc
    23821 tggaatgcct cgcaagagat ataattgaaa agtccaagat gtaccggata aagaatggcg
    23881 atatactgga ggtaaagagc attgagattc ctgaagaagc ttcttagctt actcgtaact
    23941 gtctggattg ccgtgctcgg tgttctctac atctatgttt atggcggtat agtcattcta
    24001 atcggaaatg tgatcgggaa attcaaaggg aagaaagaga gaaagacctt catttcaaga
    24061 gaggtctcta gattcggcag ggcggctttc gtgctttcgg ggtcaagagt aaggttgtct
    24121 ggtaaggaaa acgttccaga gaatggcccc atggttatcg tcgcaaatca tcaaagcgca
    24181 ttcgatatac ctcttatacc cggctacgtc tacccagaga tagctttcat tgcaaaaaaa
    24241 gagctctcaa agattcctgg cataagctgg ttcataagcg cgctggacgg agtatacatt
    24301 gaaagaggaa acagattaca aacggccggc gcaatgagga aaatatttag aattctgaaa
    24361 gaggacggaa cgattcttct ttttccagaa gggacacgaa gcgtggccgg tgaaatcggg
    24421 aaattcaaag aagggagttt atcaattccc tataaacttg gcgtgaaggt cctccccgta
    24481 gcacttgacg gaacacgcaa tcttctcagg aagaacagct ttctgatcac accttcgaca
    24541 atctctcttt ctatttgcaa acctatcctt ccccaagatt tcagttccga ggaagacttc
    24601 agcggagaag tctataatat aattaaggca gctctggaat caatgagaaa ttgaggtggg
    24661 ttctatgtta caggtaagac tgaggggtct tgctcttgac cagagtaatt ccccggtggt
    24721 aatacttgaa gtcgaaaaga ctaacaaagg tttcggaata tggatcggac cgttcgaggc
    24781 cgaagctctt gccctcgcga ttagcggaaa ggacttccca aggcccttaa catatgacct
    24841 atttgtaaat acggttactc agcttggcgg ggtatttgaa aaggcggtga tcggacaggt
    24901 taaagacaat gtttattacg cttctctgca tttgcaggat agaaccggac agctttacgt
    24961 tgtcgatgca cgtccttccg attgtcttgt gcttgccgtc aaaaagggct tcccaatttt
    25021 tgtagaagat tcggtgttta aagagagttc gatcgaccta agcaatctac agaccgacgc
    25081 tcttcagcac ggccaggaag aagataccga gggtttcaag aagtttgtcg agaatctcga
    25141 tatcgaagag ctgaaaaaac attttaggag caaggaagac gatagtgaaa ctagctgaat
    25201 tctctccctt tttcaattgc aaactcaaga actttctgga cgaactccac atatccaagc
    25261 ctttcaaaga tgtcgtcagc tatactccgc tggctggagg caagaggctc agggcatatc
    25321 taatctggga actttccagg tacacggggt ttggagagga aaacgctttg aaggctggaa
    25381 ttgccgttga gctgtttcac agcgggagcc tgattcacga tgatctacct gctatagata
    25441 acgacactat tcggcgaggc ctgccatcaa gtcatatcaa attcggtgaa tccaaggcaa
    25501 tacttgcggg agattttctt atgctttttc caggcagggt cctttcctcc cttgatcttg
    25561 aaaccagata catccatccc attctagagc tgtggcaaga gacatcactc aaagttgtag
    25621 agggtgaatt ctatgacgtc tttccagaag acgaatccag agaacagatg gaaaggatcc
    25681 atactgcaaa gacaggagct ctttttggct tctgcttcgc tgcccccttc ataagagaga
    25741 aaagagaaaa gatagacgag atgtacaaac tcggtgtgag attcgggaaa ctctttcaaa
    25801 tgatggacga cataaaggac gtcatttcta ccgaaagtga acttggaaag acacccggga
    25861 aagatgggaa acagaataga ctgactattc tatcgttcaa gtctcttgag gaggcccaga
    25921 tagaggtcaa ggagtcgttt cattcaattc tagtagaaat agactctttt accgacctca
    25981 agaaagagat gaaagatata tactatctga ttgccagaag ataaatcaaa atccaaatag
    26041 cctcgccctc aacgcttttc tgaagtgatc ggcgtctgtg tacctcttgt ctgcaactag
    26101 agacattgac ttttccagaa ttcttcgtgc cttcctgggg aatagatcta agacaagtgg
    26161 aattcccttg atcctctctg tcgaaggaat aggatcctct ccacttagga gaaagaaaag
    26221 gatacctcct acggaataga cgtcccatga atagcttgga gttgaagaag atagaaattt
    26281 ctcgggacag gtgtaactca aagaagcaac cttagaaagt gaattcatca agtaaacatc
    26341 tttcaaacag gctgcgccga aatccatgac ctttattttt ccttcatctg taagcatgac
    26401 gttggtcggc tttaagtcac catgaatgta acctctttca tggatcgagg acacaccctt
    26461 aaggatttca tcgataattt ctagagcttt agactcatca acaactccat cggacttcac
    26521 cgtgtctctt aatgttcttc cgcttataaa ttccataaca tagtaagctg tgttgttctc
    26581 cgtaaaaaga tcaagtacct tcacgaccct atcatcgtcg attttagaca tgattgtgaa
    26641 ttcttctgaa aaagaagaaa gcctttcgaa ataggcttcc ttcagatcgc tgcttatggg
    26701 tttaacgaaa ttaccttccc tttctgcacc tcttggaaag aactccttaa cagccacgct
    26761 atttccggta tgaaagtctg agcataaata ggtcatgcca aaaccgcccc ttccaagggg
    26821 ctcaataatt ctgtaacgtt tcttcaagac tgttccttcg ttgaggtagt tctgtattgt
    26881 gatcacctct agtagaataa tatcatcatt gatgtgaaag gtggtagctg aatgtttgta
    26941 gactttcact gtcattctga tcattccgac ggcacggact cggttgcggc aatagtgcaa
    27001 aaggctgcgg acaaaggaat ctccattctc tctgttaccg accacgatac actggctggc
    27061 cagaatgaag ccagagaact gtgcttgaga gctggaataa agtacataag aggagtagag
    27121 ataagctgtg actttcatga gaccctcgat gttcttggtt acggtatggt tttcgaaact
    27181 tcgtctatgg aagaagagct cagacagttg aggcttaaga gagatcttag gaacacaaga
    27241 attctctcta gacttcgatc tctgggtgtc gagattaccg acgaagagct ggaaagttca
    27301 tttcccgggg agagtctggg aagaccgcac atagccaatc taattgtgag aaagggttat
    27361 tctgattcaa cagagaatgc cttcaaagag tttcttggaa aagatggaaa agcgtacgtt
    27421 gagaaagaga agttatcaat aaagagtgca attgaactca ttctttccgc tgggggtgtt
    27481 cccgtcctag cccatcctct ttcgatgatg ctcgatcgaa acggaacggt agagattgtt
    27541 agaacgatga tgcgctatgg attgaaaggc atggaagtgt tttacaagac ctatgaagaa
    27601 gctactagag aagagctttt cgagatctca agaaacctcg gattgatagc cacgggaggc
    27661 agtgattatc acggcaagca caaaaaagat ctcgaactgg ggattgatat tccagactct
    27721 gcagttttca gcttccttga atccttaccc gccgacaacg gccttgaaaa ataggacttc
    27781 cgagtcatct ttcagaaacg tattcaaatt agcaattttc ccgttctcga tcaaaatgta
    27841 atgatcataa tcgatcgata gagaactgag cagctcgcta aatcatgttt ccttcggtat
    27901 ttatagtctc atctctaaaa ttgaccttca tgagctcttg ccgatcgaat aactggccag
    27961 ggcgatagat gccagtttag aagcagccga atcggctctg gatacaataa cgattggggc
    28021 agttgcaccc aaaataagac cggcgatcgt tccaccggca agatagacgg ccgacttgcc
    28081 cacaaaattg ccacttgcaa tatccggtac gactaggaga tcggcctgcc ctgctaccgg
    28141 cccggaaatc ttcttgattt gtgccgctgc aagcgaaagc gcattgtcca tacctagagg
    28201 cccatcgatt acacaatcgc tgatctgccc tctttggctc atcttggaaa taacggccgc
    28261 atcgatagtc tcttgcatat cttcgttcac aacctcaaca gcagcaataa gcgcgacttt
    28321 tggtttttca tagcctattg acttcagaaa ggcaactgcg ttctcgatta tcgacacttt
    28381 tgtctgtaga tccgggcgga ttatcattcc tccgtcggtt acgaaaacta ccctgtcgac
    28441 tcccggaaca tccatagccg ctatgtggct caaaaccctt cccgatctta agccccattc
    28501 tttgttcagg actgccttca ggagaatcga tgtcttcaac agtcccttca tcacaacatc
    28561 ggctttgccg gaactaacta actttatccc cttctcgcaa gcttcttcgg gggaggaggc
    28621 gggtatcacc tctccatcaa gtccttcttc tttcaagatt tcgagagtct ctgcttctct
    28681 gccaacaaaa accggcgtcg cgatcccttc gtcaaatgcg tgtttgagag cccttatccc
    28741 ctctctatct tccgatccta taagaacaac tcttcgagta ccgcctgcct tggcaagcga
    28801 aacaagttca gaaaagtctt tcaacaaaaa ccctccttga caccgatttg cacttcttcc
    28861 c
//
LOCUS       DAOQYI010000023        28622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1921_length_28622_cov_71.381839, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000023 DAOQYI010000000
VERSION     DAOQYI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 28622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28622
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_28622_cov_71.381839"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKL93_07155"
     CDS             complement(<1..413)
                     /locus_tag="PKL93_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HNQ71071.1"
                     /translation="MSRRRTGGPELNPRQIRVLYCISREYISSGKPVSSKQVLEHSNL
                     KFSSATVRNDMRKLEFLGYIYQPHTSSGRVLTDKGFRFYLDSVKTITEDLQESNVAIA
                     IRRTSFVGDIEHLLQGMTRILARAVSGFVVIEKPK"
     gene            521..2017
                     /locus_tag="PKL93_07160"
     CDS             521..2017
                     /locus_tag="PKL93_07160"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNQ71072.1"
                     /translation="MRLSQVIELLGNSVLQVVNPGNVDPEFDHIESDSRGLKERNLFI
                     CIKGTNFDSHLIARELQRKGAVALVAEMPIEDEEVVVPIVYVKSSRLVEALLTMEEYC
                     HPYRKLTTIGVTGTNGKTTITTLIYHVLSSFERKASLIGTVRNVVGENIYTNPKNTTP
                     GPVELAKLLQLSTKLKSEYFIMEVSSHSLSMNRVEGMRFDVGIISNVTRDHLDFHPTF
                     DDYYRSKMKLFSLLKTNGIAVVNGDKINIADIHISRNRITTYGFGDESDYRIENLDIS
                     RTGMDFTIQTPYGSSHRIYSRLIGEHNAYNIAAAVAALNSLNYDLDHIAKAISSFGGV
                     PGRFEFVEEATKYGFDVVVDFAHTPDALEKLLKTARRLTPGRLILVFGAGGSADKGKR
                     PLMSEVSSKYSDVVILTSDDPKLDDPAEILQDLESGVDKFKPYLVIPDRREAISVALT
                     LANRQDMVLIAGRGHEDFQILGEKRIPFNDKQVVKDILESKFRRHIKK"
     gene            2014..3339
                     /gene="murF"
                     /locus_tag="PKL93_07165"
     CDS             2014..3339
                     /gene="murF"
                     /locus_tag="PKL93_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNQ71073.1"
                     /translation="MISKSSVEEFLLQTEDRNIVIDSRKVSAGDVFIGLKGEKVDGND
                     FVGEALSKGASLVFSDKAFDDQRVLRVDDTNEILIEAAKSILSKSKLSNRIGITGSNG
                     KTTTKEISSFFLSKLGRVFKTAGNLNTEIGLPLSLLENRRELFSAQYGVFEFGTSTKG
                     DIEKLVGIVQPDIAVLLNVGSAHVGNFKDIDELLQEKLSIFKSKRLSRAVLGGCDERL
                     REFSKGLPVEVLLFGKEDVDFSIVDFAYDKGDTMLHFFCDGDRFVRLKGIWSYGQLMD
                     LGAAYLVARLVGVEEPSVFLNDYKLPFSDRFTVSILKGITVISDFYNSSLESWESAIL
                     SIEKLKSSRKIAVAGSILEQGREEKRTHERLGELLSKFDETVLFTRDMAINAASKNVK
                     PALVSDSEEEIAAWLQRNVRSGDLVFFKASRAIALEGVYKSFMELIESA"
     gene            3332..4258
                     /gene="mraY"
                     /locus_tag="PKL93_07170"
     CDS             3332..4258
                     /gene="mraY"
                     /locus_tag="PKL93_07170"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNQ71074.1"
                     /translation="MHREVFTFLLAFLLSVLAIEPLKRYQKRRRIGQYIREEGPDLHN
                     YKTGTPTAAGIVFIPIALTVASIFAFRAEILIVVIAGILFGLIGLVDDISKIVKRNAS
                     GLSGKRKLALQLVSAAFIVFLIQLVNPHTHIEIPFLGKIGLGFAYYPISALIIAGMSN
                     AVNLTDGVDGLAASVYIFSLTPMLALPVWQNGLIAPISGALAGFLWYNWFPASIFMGD
                     TGSLSLGGIMAVIFALDGREGFLIFFGLMFLVEMFSVIIQVFSFKVFGRRVFKMSPIH
                     HHFELSNWKESKIAFRFSLIAFIGAVFGLLSW"
     gene            4259..5593
                     /gene="murD"
                     /locus_tag="PKL93_07175"
     CDS             4259..5593
                     /gene="murD"
                     /locus_tag="PKL93_07175"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNQ71075.1"
                     /translation="MKKLLEGKIGLVGFGVSNEKLLERLVKDRDSKSLFVSDTGKIKE
                     EGRTLLKKFDIEYEEGGHTDRLFDCDVFIVSPGVSPFSEVGERIIHSGKYYTTELEVS
                     LDELWSKPRGTVIGVTGTNGKSTTVTMLRHIISGKNRKVFQGGNLGYPLAGVAEEVYD
                     YYVLEVSSFQLKWFAEERVRFHLSAVINLGEDHLDYHRSIDDYFKSKLRLAKMTEGIT
                     VVPKAVADCQKELLKGSKLRPFVALGEGFDSFDREHLRVRGIEFRTSDLPFTGLHNYE
                     NVLVVLLISQLIGLSAEDVFQELKSYTFLSHRLQLVRELEGVKYYDDSKATNAHAVSA
                     ALRSFEPSKTILILGGREKDETYSELIEQLGQLKHVVMLGSSMKILSAKLQMRGIPVS
                     GAANMADAVKICRGLATKGDNVVLSPAGSSYDLYINYSERGNHFREIVMALE"
     gene            5596..6711
                     /locus_tag="PKL93_07180"
     CDS             5596..6711
                     /locus_tag="PKL93_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNQ71076.1"
                     /translation="MKRTYLVLALYTSVFVIFGLVFIYSAGISMEARHPDVTASGFLE
                     KQLIAYAIGLAGALIMIYMKGSWHFKNAFTIYYPLTLIMLVAVLFFPDRGGSNRWIDI
                     GSFSLQVSEFAKISLLLVLAKHFGGLKKRNFLTTFLIPLGMTAPLAILVFIEPDLSTT
                     GIIVAIAFVMMIIGGIKMRYLSLALIFVVILVLVLYSGGFIEDYQIQRITSFLTSLTG
                     EEHEQVSYSLMAISSGGLTGKGLGMGLVKYYLPVSYSDFIFAVIGEELGLVGLLVLMF
                     AYVGFIRELIVVGLRGSRTLEGKLYIIGFALYIMIQATINIAVNLGLFPPTGVTLPFV
                     SSGGSSIMSLMIGYGLVFSILLESEEEKEKGLQDEKT"
     gene            6698..7768
                     /locus_tag="PKL93_07185"
     CDS             6698..7768
                     /locus_tag="PKL93_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNQ71077.1"
                     /translation="MKKLKIAFCGGGTGGHYYPAVAILHALSRVREIELLYFTVWGKI
                     DDRSVDRDFPGVRKMSLKLTGLKRPLYSPANASIFFSHLRTERSVKRQLAEFSPDFLF
                     STGGYVSYPVVRAAKSLEIPVYIHEQNSIVGIANKRLAKYAKRFFISFEESGRDLELP
                     KERIVFSGNPVRESKITRNEVMKRFDLPDNKPLIVVLGGSLGSELINRACEGLYEEIQ
                     SNDCELSFLHSTGDESSAISLRRFPFVRAFSYIEDLTDAIACADLVVSRGGATTIAEL
                     QYFGRKGIIIPWSGAAENHQFYNARSLERVGLGYVILEENLTSTALRIAIKEMLHRQI
                     SYKPPRRPVEIVLDNILREESI"
     gene            7765..9108
                     /gene="murC"
                     /locus_tag="PKL93_07190"
     CDS             7765..9108
                     /gene="murC"
                     /locus_tag="PKL93_07190"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNQ71078.1"
                     /translation="MKYHFIGIGGIGMSGLAMHLASEGDQVYGSNYEENERVAYLRGK
                     GIGVFIGHSYENFEKPDVVVRTTAIKQGNPELERAIFESVPTIYRMELLKSLLLRDTS
                     VCITGTDGKTTTTAMVSKILIDSGRDPTVFLGGINPILKDGNYRKGKDLIVSELDESD
                     GFFASFKPDYAVITNVRGDHLEHYDNSFDNLKNHFRYFSRSVGKLLVTNADDPASERI
                     FTDTLTFGKDRGYYRFTDRSTGIMNQTFRCFRGNEDLGLFKLMIPGEFNVYNATAAIA
                     LTHEMGVPIESIKNSLESYRSVDRRFTFRGLDDFRNLFFFDDYAHTPDEISNTIRGAR
                     EFFPGKNVIVVFQPHRYSRLVRENGRFANSLKDATEVCVFKLYEAYEKGQYAIDETEV
                     LKGLSSYGVPAVHAVNYLEILEWLEKKRDAVILFLGAGDITEASKMSVLKLCEAH"
     gene            complement(9086..10864)
                     /locus_tag="PKL93_07195"
     CDS             complement(9086..10864)
                     /locus_tag="PKL93_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ71079.1"
                     /translation="MINSDDIRKWLVSSGVLNKVGKPSRYIGKELNRVIKDPDDKLRI
                     LLSFPDTYEVGMSHLGLKILYKELNKTDEFYAERAYLPWKDMIEEMISARIPLYSMET
                     YTAAAQFDIVGITLQYELCYSNVLALLDLAGIPLLQKERTTEPLVLGGGPCSTNPEPM
                     ADYFDAFVIGDGEAVTPQICIEVRENIELLRSGRRRELLELLSKIQGVYVPSLGRRKV
                     VKAVIADLSDYEIDPSPLVPYMKTVHDRAVMEVMRGCNRGCRFCQAGMIYRPVRERGV
                     DELRESVRKVLKATGYDELSFLSLSTMDHSSIDVLSEEILPLLEPQRVALSLPSTRID
                     SFGVEIASQIASIRKTGLTFAPEAGSQRLRNVINKNVSEDDLMATVKAAKNSGWQRVK
                     LYFMVGLPTERDEDLEEIVRLARRVKAVGFKEVSVSAAIFIPKPHTPFQFSPQISEVE
                     ASRRLGILRKLSGRGIQLSCHDPSSSTVEGVLSRGDRKVGRAILRAYKSGAIFDEWNE
                     EFDASIWQEAFKTEGVDVQDYMKGYSTQDELTWDHISLGISKRFFIEEYEKALREETT
                     GDCRWVGCTGCAVCQTLNVTNVLRKA"
     gene            complement(10864..11862)
                     /locus_tag="PKL93_07200"
     CDS             complement(10864..11862)
                     /locus_tag="PKL93_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ71080.1"
                     /translation="MNLDRLTEVVFVPGMTGREEMIRAKIIEMLPEGVEPFVDDLGNV
                     VLEVGNGDSSIAFAAHMDEIGLLVTGVNSDGSLSFKKVGGIADELLVGRHLDVITSSG
                     ESIDGVIGMTPPHLREKTDLSWQNQVIDVGTSSKEETASLGIEVLDYAVFRKQISILN
                     DKYVAVRSLDDRSGCLALLDLLDMLMETDLDRRIVFAWTVQEEIGLIGARALSERLKP
                     NLFFPVDSFACCSRLTGDVKPGNGPVLRMSDTSSLGDYGLGKRLMEFSRERDIPLQMG
                     VTGGGTDGIPFQQKGKKMVPLAMAVKYLHSETEYISMEDYDNLVRLMFLISTEFTV"
     gene            12027..13691
                     /locus_tag="PKL93_07205"
     CDS             12027..13691
                     /locus_tag="PKL93_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNQ71081.1"
                     /translation="MSNRERLEKLKDDWKRERVIPLLKRFPERKKEFKTSFDGPVEIL
                     YTPKETEDYEEKIGFPGQYPFTRGVQPTMYRGRLWTMRQYAGFGTAEESNQRYRYLLS
                     QGQTGLSVAFDLPTQIGYDSDDPMSEGEVGRVGVAIDSLEDMETLFEGIPLERVSTSM
                     TINSTAAILLAMYIAVGEKQGVDPSKLTGTIQNDILKEYIARGTYIFPPDSSMRLITD
                     IFEYCSKNLPDFNTISISGYHIREAGANSVQEIAFTLADGIAYVQAAIDAGLDPNVFG
                     KRLSFFFNSHNGFLEEIAKFRAARRLWARIMKERFGVTDEKAMMLRFHTQTGGSTLTA
                     QQPMNNIVRVAFQALAAVLGGTQSLHTNSFDEALGLPTEDSVTIALRTQQIIASETGV
                     ADTVDPLGGAYFIEELTDRMEELAMDYIQKIDDLGGMVEAVRNGFVQKEILDSAYKYQ
                     VSIENKEQIIVGVNEFVSEDKEVKDILRLDPAIENRQKKKIEMLRTRRDNGKVESLLD
                     DLREAARGSDNLMPFILKAVKAYATLGEIANALRDVFGEYTEAVIL"
     gene            13707..14096
                     /locus_tag="PKL93_07210"
     CDS             13707..14096
                     /locus_tag="PKL93_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNQ71082.1"
                     /translation="MKIKVLVAKPGLDGHDRGAKVVAMALRDAGMEVIYTGLRQSPES
                     IVKAALQEDVDVVGLSILSGAHMKICSKVLKLMEDSGIGDKPVFVGGIIPPEDAEELK
                     KAGIFEVFGPGTSLKSIAEKIEKTVKK"
     gene            14102..14950
                     /gene="meaB"
                     /locus_tag="PKL93_07215"
     CDS             14102..14950
                     /gene="meaB"
                     /locus_tag="PKL93_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNQ71083.1"
                     /translation="MELKIDQLKVSRVLSIIENNTDEARKIIDKLPQRDYFVIGVTGS
                     PGAGKSSLTDRLACMSAVEKSTAVVAIDPSSPFTGGAFLGDRIRMRRATDYPGIFVRS
                     MASRGNVGGLSPSIYNTVEFLGRSGYDWIYVETVGAGQSETDIANLADIVVLVLAPGL
                     GDDVQTMKAGIMEIGDIFVVNKSDLPGSDQLFSRTRAILELSGKQLPVVRTNSIKGNG
                     IEELHKKIEEILEDYINSGRLSEKRRKRNLYNNLNSAYQIIKEHYAENAKLEELIKYL
                     LENIGR"
     gene            14956..15363
                     /gene="mce"
                     /locus_tag="PKL93_07220"
     CDS             14956..15363
                     /gene="mce"
                     /locus_tag="PKL93_07220"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNQ71084.1"
                     /translation="MRSDKIDHIGIAVRSIEERLSVYRDLLKLELTGIEELPDRGLRV
                     AFVKAGDTRIELLEPISEDSQISKFLESRGEGIHHIAFHVDNVEGAIEEAVSLNLKPL
                     SKEAEIGAGGTKVVFLHPKSTGGVLMELVEGHH"
     gene            15376..16914
                     /locus_tag="PKL93_07225"
     CDS             15376..16914
                     /locus_tag="PKL93_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNQ71085.1"
                     /translation="MTGRLEEFFEKSEKITLGGGQDKIEKHHKSGKLTARERIDALCD
                     EGSFEEFDRFVKHRATSFGLENKEFPADGVITGIGTVNGRKIAVFSQDFTVQGGSLGE
                     MHAKKIMKVQDMAMKLGIPIVGINDSGGARIQEGVDSLFGYGGIFHRNTLSSGVIPQI
                     TVICGPCAGGAVYSPAITDFIVMTDKHSQMFITGPQVIKAVTGEETSMEELGGALVHN
                     TKSGNAHFLVPDDKNAMLLVRELLEYLPQNNSEEPLKTESTVSDPVLELREIVPDNPK
                     QSYDIKKVISLVVDDGKFLEVHEHYAKNMVIGFARIGGRSVGIVANQPSVFAGSLDIN
                     ASDKAARFIRFLDAFNLPIITFVDTPGFLPGISQEHGGIIRHGAKLLYSYSEASVQKI
                     TLILRKAYGGAYIAMGSQHLGADIVFAWPGAEIAVMGPEGAANIIFKREIDASDNPEK
                     TRQEKIAEYKDVFANPFVAAGRGYIESIIDPAESRREIIRALETLDTKVEGRPSKKHG
                     NIPL"
     gene            16925..17275
                     /locus_tag="PKL93_07230"
     CDS             16925..17275
                     /locus_tag="PKL93_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNQ71086.1"
                     /translation="MPDYVQITLIGVAIVFLVLAMLYVIFNVLGKLLSGGEGHGVKAV
                     SEKTTSNSVLAPLRSIAGDEEEVIAAIAAAVNAVIGHSDFRIRSVSPVPSARASQWKR
                     REPTVYWKVRRNRN"
     gene            17272..17697
                     /locus_tag="PKL93_07235"
     CDS             17272..17697
                     /locus_tag="PKL93_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNQ71087.1"
                     /translation="MRRFIVRVNGKEYDVEIEELSSAAGSNPVSAAQKQETAQKVEAV
                     ERPRPTETVRDSEKPVEPEQQTSGSGRGINILSPMSGTILDVLVSVGDSVSPGQKVVM
                     LEAMKMENSVLSEDAGTVKEIRVKKGDNIDAGEVMIVLS"
     gene            17791..18249
                     /locus_tag="PKL93_07240"
     CDS             17791..18249
                     /locus_tag="PKL93_07240"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HNQ71088.1"
                     /translation="MDSADRNLATKARELSLSAFGVFPDIPFSFNSRLKRVLGRLVYS
                     KSREMLTPLRIEISSSISDNEELLKKTLLHELSHFYLMMNDRDFSHNSPEFRKLSQEL
                     GFDIVAEYEGLPVHIWVCSVCKRTVAISFNRRRKNGLSSCCKAPIELQEK"
     gene            18324..18563
                     /locus_tag="PKL93_07245"
     CDS             18324..18563
                     /locus_tag="PKL93_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71089.1"
                     /translation="MGTYKRLTKVLVYFHEDYFVPIKFSYDGIKIIVNAVTSFWVEAV
                     GTQKVYHFSVMTTLGAYNLEFEEDSKKWYSYTLGG"
     gene            18569..19807
                     /locus_tag="PKL93_07250"
     CDS             18569..19807
                     /locus_tag="PKL93_07250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNQ71090.1"
                     /translation="MKGIPYVSHIDMDAFFASIEQAANPTLKGKPIAVTGIGKRHSVV
                     TSASYEAKRFGVKSGMAFFEAVKLCPDLIAVGVQSKKYGYVSKEIMKMLTSVSPRVMV
                     ASVDEAYLDLSFHTRLEESINRFISFKRDLKERFGITASVGISVNPIISKIASDYSKP
                     NGFVVVKDGMEREFLNEIPVKDVPGIGRHTLIKLESMGYRTTGELLKGLDDSPMDLYT
                     MFGNAFMGFLQSLKNNRFSRKEFFKEERPKSVGHSMTLSGDISDFDLIVRVTNFLAAR
                     VIYRMGRYKMEGSGISLFLRYADRSSKALSKKLSFTISSIKDITEITPWLIRSIWNGT
                     SVRAIGISCWALSEKKNQRQLSLFEKEKELLETVLEIEKKFGEYAIFPGNILFLPDIR
                     KLEKPSDLHYQGFCRREVSC"
     gene            complement(19724..20131)
                     /locus_tag="PKL93_07255"
     CDS             complement(19724..20131)
                     /locus_tag="PKL93_07255"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNQ71091.1"
                     /translation="MSEKPRIALIAHDKKKIDLIMFVREHVDVFKKCALFATSTTGNI
                     LREKVKLELTSFKSGPLGGDLQIGSLVASDEIDFVIFLRDPLTAQPHEPDVSALLRVC
                     DVHNKPLATNLATAEALVMEIRRFLKLSDVGKE"
     gene            20300..20551
                     /locus_tag="PKL93_07260"
     CDS             20300..20551
                     /locus_tag="PKL93_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71092.1"
                     /translation="MIFGYRSARETEEIFDIRFDERNSKRVFAEIYKGCYLLIRGEVR
                     VRKSVYIRLRDFTVTTRPHDRDLDIDIPRILIHLRSDYF"
     gene            complement(20542..21258)
                     /locus_tag="PKL93_07265"
     CDS             complement(20542..21258)
                     /locus_tag="PKL93_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71093.1"
                     /translation="MKHCLVLVIVFSLLVGASLLASESVYDDLTLFIQQVVEAGDIDT
                     LVGIHFHLADTESADSGYRDLNLKARIIMVGGEDFYIEFLEPDELSKIALVYLIREEV
                     VFSVVDGDPWKQYKSTYQTDFIAKLIEDFFSGLLDSRNFTWTVSTEEERRIYMIDPSE
                     QRLKVLGLLGGGGYLPNLMHIHVSLLRKEGCFPKIEYVKITDRVGDEYLLIEIEEFKT
                     VVDRSIFTELRSKYYETFQK"
     gene            21336..22823
                     /locus_tag="PKL93_07270"
     CDS             21336..22823
                     /locus_tag="PKL93_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HNQ71094.1"
                     /translation="MRVAFPDQMRKIDKTIIEHGVPSLLLMEEAARSVFEEIRSFNLS
                     NAVVLCGGGNNGGDGYTVARLLLCAGVHVKVVQCYVPSTVDCKANATLYSSLGGQIII
                     IGEKTDVEELLSKADIVIDAVFGTGFHGELEPGVAQIFDFVNRLDVIRLAVDIPSGVS
                     GFDGSISKSCFRAHKTVTFGLAKTGHFLYPGREFCGDMVVGSAGFPKNVMDSIAEPEV
                     IDDEMVSKLIPERAPNSYKGTFGSVLVVGGSNIYTGAPYLSALGAFRSGCGMVYTYTP
                     KGSATVIRNNLPEAIAYESRGDFLIPRDIEVLPKIMEKIDSIVVGPGAGREKESIEFI
                     LKLLQSFPDKNFVVDADGLYALSNDLSVLARSERIIITPHIGEFARMNEGKTDLKSFI
                     QFCVGNRTTAILKGASSVLCDDKGKITINTTGNTGLAKAGSGDLLSGTIAGFASQTGE
                     LTKSAILAMYFMGRAAELSIVSEPCNSASQIAESYSLVFKELEQK"
     gene            22837..24027
                     /locus_tag="PKL93_07275"
     CDS             22837..24027
                     /locus_tag="PKL93_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HNQ71095.1"
                     /translation="MELNHNVSQRLEQKLGLSLKAQQALKLLQLNSMEITAELEEIVQ
                     ANPLLEMKREDYELIEGKSDLESSPEDEENTEDGDLYSLPENNSIYMKPESTDRDGWD
                     FDRIEAQQPTFREILRDFAFYILDDDLFSLFEILLDYMDDLGMLTEDLEKIARENHCS
                     VDGLSSVISTMRDGGFDGIFASSREEIEALLGPGLFPSSGYSDGAFVRYVEPDLYIEF
                     IDEKFIVTVRDYSLVLSVDESYEEIMEHGEKKAKKYLEEKLEEAKFYISALERRKATL
                     ITIGNEIVIRNAPFLLKTSEKIRPLKMNSVADKIGVVVSTVSRAVKDKFVQTPVGTFP
                     IRYFFGNLQEKEQALEVIAEIVRGNPEISDAELVTELNNRDINIARRTVNKYRNQLGL
                     RKSK"
     gene            24024..24671
                     /locus_tag="PKL93_07280"
     CDS             24024..24671
                     /locus_tag="PKL93_07280"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNQ71096.1"
                     /translation="MKTKALLFVGGQYISTMEFYFRKLNETSFVSAADSGAEMLKSLG
                     RHPDLLVGDMDSVSQETLQWCRGRGSLVLIYPPEKDDTDTQIALQALEERGFSNVEIF
                     GATGLRLDHFMGTLASIYGLRHTLKATIIEENVEIGMVSREMTLFVNRGEIWSLFPYG
                     GQQTIVSLEGFKFPLTDASLDYGKPLGVSNETVDEEIQISCKGGTALYFRWLKRY"
     gene            24671..25189
                     /gene="yfcE"
                     /locus_tag="PKL93_07285"
     CDS             24671..25189
                     /gene="yfcE"
                     /locus_tag="PKL93_07285"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HNQ71097.1"
                     /translation="MLSDTHGALSPLTRILQEVDQNTLIMHSGDFLYHGPRNPLPEGY
                     DPKGLATLLAKFSGRIVGVRGNCDSEIDLELVGLENAASALTLLINDLGIFISHGHMK
                     HEVPFERGIVISGHTHISLLRKDNGIIFMNPGSLSLPKDRTGGSYGTIDFKKGIISLR
                     NGNGFELKTLAL"
     gene            25254..25880
                     /locus_tag="PKL93_07290"
     CDS             25254..25880
                     /locus_tag="PKL93_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ71098.1"
                     /translation="MVLRWHGHSCFSLECDGKTLLIDPFDEGVGYELPEVKPDIILES
                     HQHHDHNAHNRFESGYILVNQPVSEKVHGFIIKGHPTFHDDVEGKKRGKNIIFEVTTP
                     DGFRVVHCGDLGHRLGPDLTETLKSPDILLVPVGGVYTIDAGLARELTRDLAPSYVVP
                     MHFKTKDLTFQLAGVEDFLAGEPFEQCEKLELKEKANVGTRVVVLKYR"
     gene            25894..26220
                     /locus_tag="PKL93_07295"
     CDS             25894..26220
                     /locus_tag="PKL93_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNQ71099.1"
                     /translation="MDEKRVLSLLGIGRRANKVVFGKEQLRSYLRQPFKRKSLILASD
                     TSDSIKEDWVKRCKSHGASCILLKEHDRVALGRAIGKDNISAVAIADDGLADEISKII
                     TQAGGD"
     gene            26224..26355
                     /locus_tag="PKL93_07300"
                     /pseudo
     CDS             26224..26355
                     /locus_tag="PKL93_07300"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
     gene            26350..28308
                     /gene="infB"
                     /locus_tag="PKL93_07305"
     CDS             26350..28308
                     /gene="infB"
                     /locus_tag="PKL93_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNQ71100.1"
                     /translation="MNIIVGLYEEEKKETLRKAEKAKSKIQEKEAKKLEKKLVSIEEP
                     PTEKKIPVEHKARTIKLKSNEFKLDVLAKKMNVPISKVIKDQFMNGIILRPGQILSLE
                     DAMNIAKNYEWEVELIHEQEINPFDAIVSAYSDAYKESSRLVQRPPVVTVMGHVDHGK
                     TTLLDRIRKAKVAEDEIGGITQTIGAYQVEIRNKKITFIDTPGHEAFTEMRARGAQAT
                     DIVVLVVAADDGVMPQTIEAYNHAKTANVPIIVAINKIDKPNANVDLTKQQLASKLGL
                     VPEDWGGDTVMVPVSAKTGKGVEDVLEMILLVAEVADIKCIPEGNARGIIIDSRLDKA
                     VGPLATLVIKDGVLKPGSYVVAGSASGRVKALINEYGKRVKEANPSDPVQIIGFDEVP
                     DVHSIVYAVDDLDTSRSLADFAKSQAQKEKSVTTKRHVRLEEFLSMTGTTDEKKELKL
                     ILKADSFGTVEALKQAMAKLENSDVKIEVVHSGIGSVNSSDVMLASASDAVIMGFKVK
                     PDAMARKQAEVEGVQIKVYEIIFNLIDDLKKALEGLLEPEEVDEVIGHGHIKQLFKIS
                     KVGTIAGVQINDGYVLKKGKMRVYRRNEEVADLAIEVLKHYKDDVSRVDAPKECGIKA
                     VGFDDFQEEDQLEFHSKKSVKRTIDFTE"
BASE COUNT         8228 a         6014 c         7014 g         7366 t
ORIGIN      
        1 aacttaggct tttcgatgac gacaaagccc gatacagctc gtgcaagaat cctcgtcatt
       61 ccctgcaaca gatgctcgat atctccgaca aagctggtgc gccttattgc aatagccaca
      121 ttactctcct gaaggtcttc agtaatagtc ttgactgaat caagataaaa tctgaacccc
      181 ttatctgtca aaactctacc tgacgaagta tgtggctggt agatatatcc aagaaactcc
      241 agttttcgca tgtcatttct taccgtagca gaactaaact tgagattaga gtgctccaaa
      301 acctgcttag aactaacagg ttttccagaa gagatgtatt ctcttgagat gcagtaaagc
      361 actcttatct gcctcgggtt aagttctgga ccacccgtac gtcttctaga cacttagcac
      421 tccctcctat agactgctaa taaatgttac catatgccgc ctctgttgtc aacttcagag
      481 accgatgata caatctttaa gagcgaaagg ggtgtgatca atgaggttat ctcaggtcat
      541 agaactcctc ggcaacagtg ttcttcaggt ggtgaatccc ggaaatgtcg atcctgaatt
      601 cgatcatatt gagagtgatt ccaggggtct aaaggagaga aacctcttta tatgcattaa
      661 gggtacaaat ttcgattcac acctaattgc gagggaactt caaagaaagg gagcagtcgc
      721 tttggtcgcg gaaatgccca ttgaagacga ggaagtggta gttcctatcg tttacgtcaa
      781 aagttctcgt ctagtagagg cattgcttac catggaggaa tactgtcatc cctacaggaa
      841 gcttaccact atcggtgtaa caggcacaaa cgggaagact acgatcacaa ctcttatcta
      901 tcatgtttta tcatcatttg agaggaaggc atctcttatc ggtaccgtga gaaatgttgt
      961 gggagaaaac atatatacca atcctaagaa caccacgccc ggaccggtcg agcttgcaaa
     1021 gcttcttcaa ctttctacga aactgaaaag cgagtacttc attatggaag tctcgtcaca
     1081 ttctctttcg atgaacaggg ttgagggaat gaggttcgat gtaggaataa tcagcaacgt
     1141 caccagagac catctggact ttcaccctac cttcgatgac tattacagat cgaagatgaa
     1201 actcttctct ttattgaaga ccaacggaat agctgttgta aatggtgaca agataaatat
     1261 cgcggatatc cacatttcta gaaacagaat cacgacctat ggttttggcg atgaatcgga
     1321 ttacagaatc gagaatctcg atatctcaag aacgggcatg gatttcacta tacaaacgcc
     1381 gtacgggtct tcacatagga tctactctag attgatcgga gaacacaacg cgtacaatat
     1441 agcagcagct gttgccgcac tcaattcatt gaattacgac ctggaccaca tagccaaagc
     1501 tatctcatcc ttcggaggtg ttccgggtag attcgagttt gttgaagagg ctacgaagta
     1561 cggctttgac gtagttgtag acttcgccca tactccagat gcgttggaaa aacttttgaa
     1621 gacggcaaga agactcactc caggcaggtt gattctggtt tttggcgcag gaggatctgc
     1681 cgataaggga aaacgtcctt tgatgtctga agtctcttca aaatacagcg atgtagttat
     1741 tctcacttcc gatgatccaa agctggacga tcccgcagag atccttcagg atctagagag
     1801 cggcgtcgat aaattcaaac cttatctcgt tattcctgac agaagagagg caatatcagt
     1861 tgcccttacg cttgccaatc gtcaggacat ggtcttgata gctgggcgag gccatgagga
     1921 tttccaaatc cttggcgaaa agagaattcc ctttaatgat aaacaggtag taaaggatat
     1981 tctggagagc aagttccgga ggcatataaa aaagtgatat ccaaatcatc agttgaagaa
     2041 ttcttgcttc aaacggaaga cagaaatatt gtcatcgatt ccagaaaagt ttctgccggt
     2101 gatgtcttta ttgggctgaa aggggaaaag gtcgatggaa acgactttgt cggagaggcg
     2161 ctttccaaag gggcctcttt ggtattttcg gacaaggctt tcgacgacca aagagtcttg
     2221 agagtagacg acacaaatga aatccttata gaagcagcaa agagtatact ctcgaaatcg
     2281 aaacttagta acaggatagg tattacaggt tctaatggaa agactacgac aaaagagata
     2341 agctcctttt tcctctcgaa acttggcaga gtttttaaga ctgcaggtaa cctcaataca
     2401 gaaattggat tgccgttatc tttgctggaa aataggagag agcttttttc agctcagtac
     2461 ggtgtctttg aatttgggac gagtacgaag ggggatattg aaaaacttgt cggaattgtc
     2521 cagcccgaca tcgctgttct tctcaatgtt ggaagcgctc acgtaggaaa tttcaaagac
     2581 atcgacgagt tactgcaaga gaagctatcc atattcaagt cgaaaaggct gtccagggcg
     2641 gtacttggag ggtgtgatga gagactgaga gagttctcga aagggctacc ggtggaggtc
     2701 ctcctatttg gaaaagaaga tgttgatttc tcaatagtag atttcgctta cgataaaggc
     2761 gatactatgc ttcatttctt ttgcgatggg gaccggtttg tcagactcaa aggcatatgg
     2821 agctacggtc aactgatgga tcttggcgct gcctatcttg tggctcgtct cgtcggtgtt
     2881 gaagaacctt cagtattttt gaacgattac aagttgccct tcagtgaccg gtttaccgtc
     2941 agtattctga agggaatcac tgtgataagt gacttctaca acagcagcct tgaatcatgg
     3001 gaatcggcga tcctatccat agaaaagctt aaaagctctc gaaagatcgc tgtagccggc
     3061 tctattctcg aacaggggag agaagaaaag agaactcacg aaaggcttgg tgagttgttg
     3121 tcaaagtttg atgagacagt tctctttacg agagatatgg cgataaacgc agcttcaaag
     3181 aacgtaaagc ccgcgcttgt gagtgacagc gaagaggaaa tcgctgcatg gctccaacgt
     3241 aacgtaagga gtggtgatct cgtcttcttc aaagcctcgc gtgcgatagc ccttgaaggg
     3301 gtatacaagt cgtttatgga gctgatcgaa agtgcataga gaagtattca ctttcctcct
     3361 ggcattcctt ttgtctgttc tagctatcga gcccttaaaa agataccaga aaaggagaag
     3421 gatagggcaa tacattcgcg aagaaggacc tgacctccat aactataaaa caggtacccc
     3481 aactgctgca ggaattgttt ttattcctat cgccctgaca gttgcatcaa tctttgcctt
     3541 tagagcagag atcttgatag ttgtcatcgc tgggattctt ttcggcctca taggacttgt
     3601 cgatgacatc tcgaaaatcg tgaagagaaa tgcatctgga ctgagcggaa agagaaaact
     3661 cgcccttcag cttgtctcgg ctgccttcat cgtattttta attcagcttg tcaatcctca
     3721 tacacatata gagataccct ttctcgggaa aatcggtctc ggcttcgctt actatccgat
     3781 ttctgctttg atcattgccg gaatgagcaa cgctgtaaat cttaccgatg gcgttgacgg
     3841 attggcggcc tcagtgtaca tattttcctt gactcctatg ctcgctcttc ccgtttggca
     3901 aaatggtttg atcgctccga tcagcggggc tttggctggt tttctatggt ataactggtt
     3961 tcccgcttct atatttatgg gtgatacggg atcgctctcg cttggaggaa tcatggccgt
     4021 gatttttgct ttagacggga gagagggttt tctcatattt tttggtttga tgttcctcgt
     4081 ggaaatgttc agtgtaatca ttcaggtatt ctcattcaag gtttttggtc ggagagtgtt
     4141 caagatgtcc ccaatacacc atcactttga actatctaac tggaaagaaa gtaaaattgc
     4201 attcaggttt tcattgatcg ctttcatcgg agcagtcttc ggtctgctgt cctggtaggt
     4261 gaaaaagttg cttgaaggaa aaatcggtct tgttggattc ggagtttcga acgagaaact
     4321 ccttgaaaga ttggtaaaag atagagattc caaatcgctt tttgtttctg acaccggtaa
     4381 aattaaagag gaaggtagga cgctcctcaa gaagttcgat atagaatacg aggaaggggg
     4441 ccatacggat cgtctctttg actgcgatgt cttcatcgta tcccctggag tctcgccgtt
     4501 ttccgaagtt ggagagagaa ttatccattc ggggaaatac tacacgaccg agcttgaagt
     4561 atccctagat gagctttggt ccaagcccag agggacggtg atcggcgtca cgggaacaaa
     4621 tggtaagtca acaaccgtta caatgctcag acacattatt tccggaaaga atcgaaaggt
     4681 ctttcagggc ggaaacctcg gttaccctct cgcaggtgtg gcagaagagg tttatgatta
     4741 ctacgtgctc gaagtcagtt ccttccagct gaagtggttt gcggaggaac gagtccgctt
     4801 ccacttgtcg gccgtaataa accttggaga agaccatcta gattatcaca gaagcattga
     4861 cgactatttc aaatctaaac tgcggttggc aaagatgacc gaaggtatta ctgttgttcc
     4921 aaaggcggta gccgactgtc aaaaagagct tttgaaaggc tctaagctcc gtccgtttgt
     4981 agcacttggg gaaggatttg acagttttga cagagaacat ttgagagtga gaggcattga
     5041 gttcagaacg tcagatcttc catttaccgg tctgcacaat tacgaaaatg ttctggtagt
     5101 gttattgatt tcgcagctta taggtctgtc cgccgaggat gtatttcagg aactgaaaag
     5161 ctataccttt ctctctcaca gattgcagct tgtgcgagag ctagaaggag taaaatacta
     5221 cgacgattca aaagcaacta atgcacatgc tgtcagtgca gccttaagga gcttcgagcc
     5281 ttcaaagact attcttattc ttggtggaag ggaaaaggac gagacttact cggaactgat
     5341 cgaacaactc ggtcaactta aacacgtggt catgcttggg agttcaatga agatattatc
     5401 tgctaaactt cagatgaggg gaataccggt ctccggcgcg gcaaacatgg ccgacgcggt
     5461 aaaaatatgc aggggacttg ccacaaaggg cgacaatgtt gttctcagtc cggcgggatc
     5521 cagctatgac ctttatatca attatagcga gagaggaaac cactttagag aaatagtaat
     5581 ggcactggag tgagtatgaa gaggacctat ttggtgctgg cgctgtacac cagtgtcttc
     5641 gtgatttttg ggctggtgtt catttatagt gccgggataa gcatggaggc gagacacccg
     5701 gacgtgactg cttccgggtt tttagagaag cagctgatag cttatgcaat aggtcttgcg
     5761 ggagctctca taatgatata tatgaaaggt tcctggcatt tcaagaacgc ctttaccatc
     5821 tattatccgc ttacgcttat tatgctggtt gccgtgctct tttttccaga caggggtggg
     5881 tcaaacagat ggatagatat tggcagcttc tcgctccaag tctcggagtt tgcaaagatc
     5941 tctttgcttc tagttctagc taaacatttc ggaggtttaa agaagagaaa tttcctgact
     6001 acttttctga tccctctagg aatgactgcg ccacttgcca tactcgtatt tattgagccg
     6061 gatctctcta ccactgggat tatcgttgca attgcctttg tcatgatgat aatcggcggt
     6121 atcaaaatgc gttatttgtc gctagcactt atcttcgttg ttattttggt gctagttctg
     6181 tacagtggcg gattcataga ggactatcag attcagagga taacctcctt cttgacttct
     6241 ttaactggcg aggaacatga gcaagtctca tactccctta tggcgatttc ttcgggcggt
     6301 ctcaccggta aaggtcttgg tatgggactt gtcaaatatt acctgcccgt aagctactcc
     6361 gacttcattt ttgcagttat tggagaggaa ctcggtctcg ttggattgct tgtgctaatg
     6421 tttgcctatg ttggctttat aagagagctt atcgttgtcg ggctcagagg ctcgagaaca
     6481 ctcgaaggca agctctacat aattggattc gctctttaca taatgatcca ggctacgatt
     6541 aatattgccg taaatttggg tctctttcca cctactggtg tcacactgcc cttcgtaagt
     6601 tcggggggct cttcgataat gtcattgatg attggttacg gactggtctt ctcaatactc
     6661 ttagaatctg aagaagaaaa agaaaaggga ctccaggatg aaaaaactta aaattgcctt
     6721 ttgtggcggg ggaaccggag gacactatta tcctgcagtt gcaattctgc atgccctgag
     6781 tcgtgtaaga gaaatagagc tgttgtattt cacggtttgg ggaaagatag atgatagaag
     6841 tgttgataga gatttccccg gggtaaggaa gatgtcactg aagctcactg gactgaaaag
     6901 gcccctgtac agtcctgcca atgccagtat tttcttttca catctcagaa ctgaaaggtc
     6961 tgtaaagaga cagcttgccg aattcagccc cgatttcctt ttttcaacgg gcggatacgt
     7021 ttcctatcca gttgtcagag cggcaaagag tcttgaaata cccgtttaca ttcatgaaca
     7081 aaactcgata gttgggatag caaacaagag attggccaaa tacgcaaagc ggttcttcat
     7141 ttcatttgag gagagtggaa gagatcttga acttccaaaa gaaaggattg ttttttctgg
     7201 aaaccctgtg agagaatcga agatcacaag aaatgaagta atgaagagat tcgatcttcc
     7261 ggataataag cccctcattg tagtgctcgg tggaagtctt ggatccgaat tgataaatag
     7321 agcgtgtgaa gggctttacg aagagattca gagcaatgat tgcgaactca gttttcttca
     7381 ctcaaccggc gatgaaagct cggcaatttc gctcagacgg tttccttttg tgcgagcctt
     7441 ttcttatata gaagacctta cagacgccat agcgtgcgcg gatctagttg tgtcaagagg
     7501 aggagctact acaatcgccg agctacagta ttttggaaga aagggtataa taataccgtg
     7561 gagcggcgcg gcagaaaacc accagtttta caatgctcgc tcactggaaa gagttggact
     7621 tggttatgtt atactggaag agaatttgac ttccacagct ttgagaattg caataaagga
     7681 aatgttgcat agacagatca gctacaaacc tccaagaaga ccagttgaga ttgtattgga
     7741 taatatactc cgggaggaat cgatttgaaa tatcacttta ttggcattgg cggaattggt
     7801 atgagcggtc tggcaatgca cttagcatct gagggtgacc aggtttatgg atcgaactat
     7861 gaagaaaatg aaagagtagc ttaccttcgg gggaagggta ttggtgtttt tataggtcat
     7921 tcctatgaga acttcgagaa accggatgtc gttgtaagaa cgacagccat aaaacagggc
     7981 aatcccgaat tggaaagagc tatctttgag agcgttccca caatttacag gatggagctt
     8041 ctgaagagtc ttcttttgag agatacttct gtttgtataa ccggaactga tggaaagacg
     8101 accaccactg caatggtttc aaagatactg atagattctg gaagagatcc tacagtcttt
     8161 ctcggcggca ttaatcccat tttgaaggac ggaaactaca gaaagggcaa agacttaatc
     8221 gtcagcgaac tggacgagag cgacggcttc tttgcttctt tcaagcccga ctatgccgtg
     8281 ataacaaatg taagaggcga tcacctcgag cactacgaca actcatttga caatctcaag
     8341 aaccacttca gatacttctc cagaagtgtg ggaaaactac tggtgacaaa cgccgatgat
     8401 cctgcttcag agaggatttt cacggatact ctaacgtttg gtaaagacag gggctactac
     8461 cgcttcacgg atagatcaac cggaataatg aatcagactt tcagatgttt tagaggtaat
     8521 gaagaccttg gtcttttcaa gttaatgatc cctggagagt tcaacgtata caatgccaca
     8581 gcagccattg ctttaaccca tgaaatgggg gttccaattg aatccatcaa aaattctctt
     8641 gaatcgtata gatctgtgga cagaaggttc acgttcagag ggctcgacga tttcagaaac
     8701 cttttcttct tcgatgatta tgctcatacc cccgatgaga taagcaatac cattcgagga
     8761 gctcgagagt tctttcccgg taaaaatgtc atcgtggttt tccaacccca taggtactca
     8821 agactggtaa gagagaacgg ccgtttcgct aactccctaa aggacgctac ggaagtttgt
     8881 gtctttaagc tctacgaagc ctatgaaaaa ggacagtatg cgattgacga gaccgaggtc
     8941 ttgaagggtc ttagcagcta tggagttcct gctgtccacg cagttaacta cttggaaata
     9001 cttgagtggt tggaaaagaa gagagacgcg gtaatccttt ttcttggtgc gggagatatt
     9061 actgaagcct caaagatgag cgttctcaag ctttgcgaag cacattagtg acatttagag
     9121 tctgacagac ggcgcagcct gtgcaaccca cccatctgca atcgccggtc gtctcttctc
     9181 ttagggcttt ttcatattcc tcgataaaaa aacgttttga aataccaagt gaaatgtggt
     9241 cccaggtaag ttcatcctga gtagagtagc ctttcatata gtcttgtacg tctactccct
     9301 ctgtcttgaa ggcttcttgc catattgaag catcgaattc ctcgttccac tcgtcaaaga
     9361 tcgctccaga cttgtaagcc cttagtattg ccctgcctac ttttctgtcg cccctactta
     9421 gcacaccttc aactgtactg ctggatggat cgtggcagct gagctgtatt ccccttcccg
     9481 acagctttct caagattcca agccttcttg aagcctctac ctcgctaatc tggggtgaga
     9541 actggaacgg tgtatggggt tttggtatga agattgcagc agaaacagat acttctttaa
     9601 aaccgacagc cttaactctt cttgccaatc tgactatctc ttcaagatct tcatccctct
     9661 cggtaggcag accaaccata aaatatagct tcactctttg ccagccacta tttttggcag
     9721 ccttaacggt agccatcaga tcgtcctcgg aaacattttt gtttattaca tttctgaggc
     9781 gctgagaacc tgcttccgga gcaaatgtta ggccggtctt tcttattgac gcgatttgtg
     9841 aggctatctc gacaccaaaa gaatctattc tagtactggg aagagaaagc gcaactctct
     9901 gcggctcaag caaaggcagg atctcttcgc tcaagacatc tattgaggag tgatccattg
     9961 tcgagagtga gagaaaggag agctcatcgt atccagttgc cttgagtacc ttccgcacgg
    10021 attcccttag ctcatcgact ccacgttctc taacaggcct gtaaatcatc cccgcctggc
    10081 agaatctgca gccgcgatta caaccccgca tcacttccat gacagctctg tcgtgaacgg
    10141 ttttcatgta aggaaccagt ggagaggggt ctatctcgta gtccgagagg tcggcaatga
    10201 ctgctttgac gacctttctt cttcctagtg aaggaacata aactccctga atctttgata
    10261 acagttccag aagctccctt cttctgccag atctaagtag ttctatgttt tctcttactt
    10321 caatgcaaat ctgaggtgtt acggcttctc cgtctccaat gacaaaggcg tcgaaatagt
    10381 cggccattgg ctctgggttg gtcgaacatg gaccgccacc caacactagt ggttccgtcg
    10441 ttctttcttt ctgaagaaga ggaatgcctg caagatcaag cagtgcgagc acattactat
    10501 agcagagctc gtactgaaga gttattccaa cgatgtcgaa ttgggcagcg gcagtgtatg
    10561 tttccattga ataaaggggg atccgagcgc tgatcatttc ctcgatcatg tccttccacg
    10621 gaaggtaagc tctctcggca tagaactcat cggttttatt gagctcttta tagaggattt
    10681 tcagacctaa atgtgacata ccgacttcgt atgtgtctgg gaaggagaga aggatacgca
    10741 gcttgtcgtc tggatcttta atgacgcgat tcagttcctt gccgatgtac cggctaggtt
    10801 ttccgacttt gttcagcacg cccgaagaga ctagccattt cctaatgtcg tcagagttaa
    10861 tcatcaaacc gtgaattcag tggaaataag aaacatcaat ctaacgagat tgtcgtagtc
    10921 ttccatcgaa atatattccg tttcggaatg aagatactta acagccatgg cgagtggaac
    10981 catctttttc cccttctgct ggaagggaat tccatcagtt cctccgccgg ttactcccat
    11041 ctgaagagga atatcccttt ctcttgaaaa ctccatcaat cgttttccga gaccgtaatc
    11101 acccagggat gaagtgtccg acattcttag cacgggtcca ttaccgggtt taacgtcacc
    11161 tgtaagcctt gaacagcatg caaaagaatc aactgggaaa aagaggttcg gtttcagcct
    11221 ttcggatagt gcccttgctc caatcaatcc gatctcttcc tgtacagtcc aggcaaaaac
    11281 gatacgcctg tcgagatcgg tctccataag catatcgaga agatccagaa gggcaagaca
    11341 gccagatctg tcatcgaggg atctgactgc cacatattta tcgttaagta ttgaaatctg
    11401 ttttctgaat accgcgtaat caagaacctc tattcccagt gatgcagttt cttcctttga
    11461 tgaagtgcca acatctatta cctggttctg ccaggaaaga tcggtttttt ctcggaggtg
    11521 aggaggtgtc atacctatca ctccatctat ggactcgccg gaggaggtta ttacatccag
    11581 gtgtcttcct actagaagct catcggcgat tcctccaacc ttcttgaagc tcaatgaacc
    11641 gtcggaattc acaccagtta ctaacaatcc gatttcatcc atgtgagctg caaaagcgat
    11701 tgatgaatct ccgttgccta cttctaaaac aacatttccc agatcgtcca caaagggttc
    11761 tacgccctcg ggaagcatct cgataatctt tgctctaatc atctcttctc ttccggtcat
    11821 gcccggaaca aatacaactt ccgtaagcct atccaaattc atgaaaacac ctccctaaca
    11881 attatagcct aaggccaaga agttaggaag ttaaactcac aaagacgttc aggacggtta
    11941 taatcaaaga tgatagcatt tgactgagga aaataattgg tctatcttag aaccattatt
    12001 cagaaccaat gaatggagga gattttgtga gcaatcgaga gagactggaa aaactcaaag
    12061 acgactggaa gagagaaaga gtcatacctc tcctgaaaag gttccccgaa agaaagaagg
    12121 agttcaagac atcatttgat ggaccggttg agattctcta tacacctaaa gagaccgaag
    12181 attacgaaga gaaaatcgga ttccccggcc agtatccctt cacacgggga gttcaaccaa
    12241 ctatgtatcg cggaagacta tggacaatga gacagtatgc aggctttgga acagccgaag
    12301 agtctaacca gagatacagg tatcttctct ctcagggaca aactggcctt tcggtggcat
    12361 ttgatcttcc gactcagatt ggatacgact cagatgaccc gatgtcggaa ggcgaagtag
    12421 gaagggttgg agtcgcgatt gactcgcttg aggacatgga gacacttttt gaaggaattc
    12481 ccctcgaaag agtcagtact tctatgacta tcaactccac agctgcgatt cttcttgcga
    12541 tgtacatcgc cgttggcgaa aaacaaggtg tggatccttc aaagctgaca ggaacgattc
    12601 aaaacgatat tctcaaagag tacatagcca ggggaaccta catttttcca ccagattcat
    12661 ctatgaggtt gatcacagac atcttcgaat actgctcaaa gaacctcccc gatttcaata
    12721 ctataagcat tagcggatat cacataagag aggccggagc caattcagtc caggagatcg
    12781 ccttcacttt ggctgacggt attgcctacg tgcaagcggc catagatgct ggactcgatc
    12841 caaatgtttt tggaaagaga ttgtccttct tcttcaactc acataatggg ttccttgagg
    12901 agatagcgaa attcagagca gcgagaagac tttgggccag aattatgaaa gaaagattcg
    12961 gtgtaactga tgaaaaggca atgatgctga gattccacac tcagactgga ggatccactc
    13021 tgaccgccca gcaacccatg aacaatattg taagagtggc atttcaggca ttagcggccg
    13081 ttcttggagg cactcaatca cttcacacaa attcattcga cgaggctctc ggtttgccaa
    13141 cagaagactc cgtcacgata gccctcagaa cgcaacaaat aattgcttcc gaaaccggcg
    13201 ttgcagatac tgtcgatcca cttggcggag cttacttcat agaggagctc acagacagaa
    13261 tggaagaact ggcgatggac tacattcaga agatcgatga ccttgggggc atggttgagg
    13321 ccgtccgcaa tggatttgtg caaaaggaga tccttgacag cgcctacaag tatcaggttt
    13381 ctatcgaaaa caaagaacag ataatcgtag gtgtaaatga gtttgtaagt gaagacaagg
    13441 aagtcaaaga tattctcagg ctggatcctg caatcgaaaa caggcagaaa aagaagatcg
    13501 aaatgctcag aacgagaaga gacaatggca aagtcgagag tctgcttgac gatctcagag
    13561 aggcggcaag gggtagtgat aacctcatgc cgttcatact taaggctgtc aaagcctatg
    13621 ctactctggg agagatcgcg aatgctctta gggacgtttt cggagaatac accgaagccg
    13681 ttattctcta gagaggggtg gagacgatga agatcaaggt actagtggca aagccagggt
    13741 tagatggaca cgatagggga gcaaaggttg ttgctatggc gctcagggat gcgggaatgg
    13801 aagtgatata cacgggtctc aggcaatctc ccgaatctat tgtgaaagcc gcccttcagg
    13861 aggatgtcga tgtcgtagga ctctccatac tctcaggcgc tcacatgaag atatgcagca
    13921 aagttctaaa gctgatggaa gattcgggaa ttggagataa gcccgtcttt gttggtggaa
    13981 ttatccctcc agaagacgcc gaagagctta agaaggcagg gatcttcgag gtttttggac
    14041 cgggaacttc cctgaaatct atcgcagaaa agatcgagaa aacggtaaag aaatgagatc
    14101 catggaacta aagatagatc agttaaaggt ttccagagtt ttgagtataa tagaaaacaa
    14161 cacggacgaa gccaggaaga taatcgacaa actacctcaa agagactact tcgtaattgg
    14221 cgttacaggg agcccgggag cagggaaatc atccttaacc gaccgccttg catgtatgag
    14281 tgcagttgag aagtcgacgg ccgttgttgc gatagatcct tcaagcccgt tcacaggagg
    14341 agcctttctt ggggacagga taaggatgag aagggctacc gattatcccg gaatttttgt
    14401 taggtcgatg gcgagtcgcg gaaatgttgg tggactgagt ccttcgatat acaatacggt
    14461 tgagtttctc ggtagaagcg gttacgactg gatttatgtt gagacagtag gagccggcca
    14521 gtctgagacg gatatcgcaa atctcgccga tatagtggtt ctcgtattgg caccgggtct
    14581 tggagacgat gtacagacaa tgaaggccgg tattatggag atcggtgaca tatttgttgt
    14641 aaataagagt gatcttcccg gctccgatca gctgttttcg agaacaagag caatccttga
    14701 actctctgga aagcaattac ctgtcgtgcg aacgaattcc atcaaaggca acggaattga
    14761 agagcttcac aagaagatcg aagaaatttt ggaagattat ataaattcgg gaaggctatc
    14821 cgaaaagcga agaaagcgca acctttacaa taacttgaac agcgcgtacc agattataaa
    14881 ggaacactac gccgaaaatg ctaaacttga agagttgata aaatatcttc ttgagaatat
    14941 cgggaggtag tcgaagtgag atccgacaag atagaccata taggcatagc cgttagatca
    15001 atcgaggaaa gactttctgt ttacagagat ctgttgaagc tcgaactaac cgggatcgaa
    15061 gaacttcccg accgtggttt gagagttgcc ttcgtgaagg ccggtgatac gagaatcgag
    15121 ctactcgaac cgatatctga agacagccag atatcaaaat tcctcgaaag caggggagag
    15181 ggaattcacc acatagcctt tcatgtagac aatgttgaag gtgcgatcga agaagctgtt
    15241 tcactgaatt tgaaaccgct ttcgaaggag gccgagattg gtgcaggagg aacgaaagtg
    15301 gtatttctcc acccaaagag cacaggcgga gttctaatgg aacttgtcga aggacaccac
    15361 tagggggcga acgatgtgac cggcagactt gaagaattct ttgaaaaaag cgaaaagatc
    15421 accctaggcg gtggtcagga caagattgaa aaacatcaca aatctggaaa gcttactgcc
    15481 agggagagaa tcgatgctct ctgcgatgaa ggaagctttg aagaattcga cagatttgtg
    15541 aagcataggg ccaccagttt tggtcttgaa aacaaggaat ttcccgccga cggcgtgata
    15601 accggcatag gaaccgtcaa cgggcgaaaa attgccgtct tctctcaaga cttcacggta
    15661 cagggcggct cccttggaga gatgcacgcg aaaaagataa tgaaggtaca ggacatggcc
    15721 atgaagcttg gcataccgat cgtcggcata aacgattccg gtggtgcgag aattcaggag
    15781 ggcgttgatt cactgtttgg ctacggtgga atcttccaca gaaacactct ttcctcagga
    15841 gtcattcctc aaataacggt aatctgcggt ccatgcgctg gaggagccgt ttattcgccg
    15901 gcaatcaccg atttcattgt aatgacggac aaacactctc agatgttcat cacagggccg
    15961 caggttataa aggccgttac cggtgaagag acctctatgg aagaactcgg gggagcactc
    16021 gtacataata ctaagagcgg gaatgctcat ttcctagtac cagacgataa gaacgcaatg
    16081 ctcttggtaa gagagttgct ggaatacctt ccgcagaata actcggaaga gcctttgaag
    16141 acagaatcga ccgtcagtga tcccgttctg gaacttcgag aaatagtccc ggacaatccg
    16201 aagcagtcat acgacataaa gaaagttatc tctcttgtcg tagatgatgg taaatttctt
    16261 gaagtacacg aacactacgc caagaatatg gtcataggtt ttgcaagaat tggcggtaga
    16321 tccgtaggaa ttgtggcaaa tcagccctca gtatttgcgg gttctctaga tataaatgct
    16381 tccgataaag ccgcaagatt cataagattt ctcgatgctt tcaatttgcc tattatcaca
    16441 ttcgtcgaca cgcccggctt cctccccggg atatctcagg aacacggagg aataatccgt
    16501 cacggagcca agctccttta ttcttacagc gaggcctccg tccagaagat tacgctaatt
    16561 ctgcgaaagg cctacggagg ggcatatatt gcaatgggca gtcaacatct cggtgcggat
    16621 atcgtttttg cttggcccgg ggcagaaatc gccgtgatgg gccctgaagg agcagctaat
    16681 atcatattca agagagagat agatgcttcc gacaaccccg agaaaactag acaggaaaag
    16741 atagcagaat acaaagatgt ttttgccaat ccctttgtcg ccgccgggag aggctatata
    16801 gaatcgataa tagacccggc tgaaagtcga agggagatta tcagggccct ggagacactg
    16861 gacacgaagg tcgaaggcag accatcgaag aagcacggga atattccgct ttgaggtgca
    16921 tctgatgcca gattatgttc agataaccct cataggagtg gccattgttt ttcttgtcct
    16981 cgcaatgctg tacgtaattt tcaatgttct tggtaaactg ttgtccgggg gagaggggca
    17041 cggagtgaaa gccgtcagtg aaaagaccac ctccaactct gtattggcac cacttaggag
    17101 tatagcgggc gatgaggaag aagttattgc ggcgatagcc gcggcggtca acgccgtaat
    17161 tggtcacagc gatttcagaa ttagaagtgt ctctccggtt ccttcggcca gagcttcgca
    17221 atggaaacgt agagaaccga ccgtctactg gaaagtgagg aggaacagaa attgagaaga
    17281 ttcatagtgc gagttaacgg taaggaatac gacgtcgaga ttgaggagct ttcttcggca
    17341 gccggttcta atccggtgag cgcagctcag aaacaggaga ctgctcaaaa ggtagaagca
    17401 gttgaaaggc ccagacctac ggagacggtt agggactctg aaaaaccggt tgaacccgaa
    17461 cagcaaacca gtggatcggg aagagggatc aacatacttt ctccgatgtc aggcactata
    17521 ctcgacgtgc tcgtatctgt tggagattcg gtctcccccg gtcagaaagt tgtgatgctg
    17581 gaagccatga agatggagaa cagtgttctt tcagaagatg caggtactgt gaaagagatc
    17641 agggtaaaaa aaggagataa catagatgca ggcgaagtaa tgatagtact gtcataggag
    17701 cggtcagaac cgctcctaat gtttttgcat gagcaactac ttttcgcagt tgatctcgtg
    17761 caagccgttc ttcttcagaa gggagataag atggattcag cagacagaaa ccttgctaca
    17821 aaggctagag agctttccct tagtgccttc ggggtctttc ccgacatacc cttctccttc
    17881 aactcaagac ttaagagggt acttggcagg cttgtttact ctaagagccg tgagatgctg
    17941 acgccgctga ggatcgaaat ctcttcctct atatccgaca atgaggagct tcttaagaaa
    18001 accttgcttc acgagctctc ccacttctat ttgatgatga acgacaggga cttctctcat
    18061 aattctccag agttccgcaa gctctctcag gagttgggat tcgatattgt ggccgaatat
    18121 gaaggacttc cggtacacat atgggtatgc tccgtatgca agaggacagt cgcaatttcc
    18181 ttcaaccgta gaaggaagaa cggtttgtct tcttgttgta aagctcctat agaacttcaa
    18241 gaaaaataat tctaacaata cttgcctttt tgaaaattca tctgttataa ttatttatac
    18301 ctaatatcta aatggtgata tttatgggga cctataagag gcttacaaag gtgcttgtat
    18361 attttcatga agattatttt gttccgataa aattcagtta tgatggtata aaaataatcg
    18421 taaatgctgt cacttctttt tgggtggagg ctgtgggaac ccagaaagtc taccattttt
    18481 ccgtaatgac gacacttgga gcttacaatc tcgaattcga agaagattca aagaagtggt
    18541 acagctatac tcttgggggt tgattatctt gaaaggaatt ccctatgtat cccatataga
    18601 tatggatgcg tttttcgcaa gtatagagca ggcggcaaat ccaactttaa agggcaaacc
    18661 gatagccgta acgggaatcg gaaagaggca ttcggtagtg acttcggcga gttacgaggc
    18721 taagagattc ggggtcaaat ctggaatggc cttcttcgag gctgttaagc tctgtcccga
    18781 tctgatagct gttggagtac agtcaaagaa atacggctat gtctcgaaag agatcatgaa
    18841 gatgttgacg tcggtatcgc ccagggtaat ggttgcaagt gtggacgagg cttatctaga
    18901 cctgagcttt cacaccaggc tggaggaatc cataaataga tttatttcct ttaaaagaga
    18961 tttgaaagag agatttggaa taacggcctc cgttggaata tcggtaaacc cgataatatc
    19021 taaaatcgca agtgattatt caaagccaaa tgggtttgta gttgtcaaag atggtatgga
    19081 aagagagttt ttgaacgaga tacccgtgaa agacgttccg ggaatcggaa gacacacact
    19141 aataaagttg gaatctatgg ggtacagaac gacaggggag cttttaaaag ggttggatga
    19201 ctctccgatg gatctttaca caatgtttgg gaacgccttc atgggttttc tccagagcct
    19261 taaaaacaac aggttttcca gaaaggagtt tttcaaagaa gaaagaccaa aatctgtggg
    19321 tcattcaatg acactttcgg gagacataag cgatttcgat ctcatagtta gagtaactaa
    19381 tttcctggcg gcaagggtaa tctacagaat gggaagatac aagatggaag gttccggtat
    19441 cagtctcttc ttgagatatg cagatagaag ctccaaggct ctttcaaaga agttgagttt
    19501 cacaatttca tcgataaagg atattacgga gatcaccccc tggcttatca gatcaatctg
    19561 gaacggtaca tctgtgaggg caatagggat atcgtgctgg gccctaagcg aaaagaagaa
    19621 tcaaagacag ctctctctct ttgaaaaaga aaaagaactt ctggaaactg tgctggaaat
    19681 agagaagaag tttggggaat acgctatatt cccgggaaat atactattcc ttcccgacat
    19741 cagaaagctt gagaaacctt ctgatctcca ttaccagggc ttctgccgtc gcgaggttag
    19801 ttgctaacgg cttattgtga acatcgcaca ctcttagaag cgcgcttaca tccggttcat
    19861 gaggttgagc cgttagcggg tctctcaaaa agatgacgaa atcgatttca tcgctggcaa
    19921 ccagggagcc tatttgaaga tctcccccca aggggcctga tttgaaggaa gtgagctcta
    19981 gtttcacttt ttcccttagg atgttaccgg tcgtgctggt tgcaaaaagg gcgcactttt
    20041 tgaagacatc tacgtgctcc cttacgaaca ttatcagatc tatctttttc ttatcgtgag
    20101 ctattaaagc aattcgtggt ttttcgctca tggctacctc cgtcatctga caagaagatg
    20161 ataacacatt cgggccatcg aaatttcaag cggttagctg aggtgagtcg atctgtccct
    20221 ttcatttaac gagataacac tatacggaga ggcaataaca gacccggaat caactgtccg
    20281 gggaggattt tttagagtaa tgattttcgg ttacagaagt gcaagggaga cagaggagat
    20341 cttcgatatc cgttttgacg aaaggaattc aaaaagagtc tttgccgaga tatataaagg
    20401 ttgctatctg ttgatcagag gtgaggtaag ggtaagaaag agcgtatata taaggctcag
    20461 agatttcact gtaacaacca gacctcacga cagagaccta gacatagaca ttccaagaat
    20521 actgatacat ctaaggagcg attacttctg aaaggtttcg tagtatttac ttctgagttc
    20581 agtgaagatc gatctatcga ctacagtctt gaactcttct atttcgatga gaagatactc
    20641 gtcgcctacc ctgtcggtta ttttcacgta ctctattttc ggaaagcagc cttcttttct
    20701 cagcaaagag acgtggatat gcataaggtt tggaagataa cccccaccac cgagaaggcc
    20761 gagaactttc agcctttgct cggaaggatc gatcatataa atccttctct cctcttcggt
    20821 acttactgtc caggtgaagt ttctcgaatc aaggagaccg ctgaagaaat cctcaattaa
    20881 cttcgcaata aaatcggtct ggtaagtaga tttgtactgc ttccaggggt cgccatccac
    20941 aacgctgaag accacttcct ccctgataag ataaaccaga gctatcttac tcagttcgtc
    21001 cggctcgaga aactctatgt aaaaatcctc accaccgacc atgataattc ttgccttcaa
    21061 attcaggtct ctgtatcctg aatcggcact ctctgtatcg gctaaatgaa aatgaatccc
    21121 aaccaatgtg tctatatcgc ccgcttcaac gacttgctgg ataaacaggg tcagatcatc
    21181 atatacgctt tcagaagcga gcaacgatgc tccaacaagc aaagaaaaaa cgatgacgag
    21241 taccaagcaa tgtttcatct tgtccacctc ttcgcgagat tataacatgt caggagaaag
    21301 agaaaagtga taagatctga ttgaggggtg atgatttgag agtagcgttt ccagaccaga
    21361 tgaggaaaat agacaaaact ataatagagc atggagtacc ctccttactt ctaatggaag
    21421 aagcggcccg cagcgtcttc gaagagatta gaagcttcaa cttgagtaat gcagtcgtcc
    21481 tgtgtggtgg aggtaataac ggaggagacg gatacacagt tgcaaggctc cttctatgtg
    21541 ctggagttca tgtaaaagtt gttcagtgct atgttccctc gacggtcgac tgtaaagcga
    21601 acgcaacttt atacagttcg cttggcggcc agatcataat aatcggagag aagaccgacg
    21661 tggaggaact cctttcaaaa gctgacatag taatcgacgc agtctttggg actgggtttc
    21721 acggagagct cgaaccagga gtcgcacaga tatttgattt tgttaatcga ctggacgtaa
    21781 tcaggctggc cgtggacatt ccctccggag tgtctggatt cgatggcagc atttcaaaat
    21841 cctgctttag ggctcacaaa acggttacct ttggattggc caagacaggt cattttctct
    21901 atccggggcg cgagttctgc ggcgatatgg tggtaggaag tgcaggcttt ccaaagaatg
    21961 tgatggattc tattgctgag ccagaagtca tcgatgatga gatggtttcg aaactcattc
    22021 cggaaagagc tcctaacagt tacaaaggca cgttcggttc cgtgcttgtt gtagggggaa
    22081 gcaacattta tacaggtgct ccgtatctct ccgctctagg ggcgttccga tctggttgtg
    22141 gaatggtcta cacatacacc cctaagggat ctgcaaccgt tataagaaac aaccttcccg
    22201 aggccatcgc ttatgaaagt cggggggact tcctcatccc aagagatatc gaggtacttc
    22261 caaaaatcat ggaaaaaatc gactcgattg ttgttggtcc gggtgccgga agagaaaaag
    22321 aaagtattga gttcattctt aaattgctcc agagtttccc ggataagaat ttcgtcgtag
    22381 acgcagatgg actttacgct ctctcaaatg atctctcggt cctcgcccga tctgaaagaa
    22441 tcataataac gccgcacatt ggtgagtttg cgcggatgaa cgaaggtaag actgatctca
    22501 aatccttcat tcagttttgc gttggtaaca gaacaactgc cattctaaag ggggcttcgt
    22561 ctgtactgtg tgatgacaaa ggaaagataa ccataaacac gacggggaat accggtctcg
    22621 caaaggcagg gagcggagac cttctttcag gaactattgc cggtttcgct tcacagacgg
    22681 gcgagctgac caaatcggcg atacttgcta tgtatttcat gggcagagcc gcagagctat
    22741 cgattgtttc agagccctgc aactcggctt ctcaaatagc cgagtcttac tcactggttt
    22801 ttaaggaact ggaacagaag tgaaggggaa gaattaatgg aactcaatca taatgtctct
    22861 caacgtctcg aacaaaaact cggactttct ctcaaagctc agcaggctct gaagctcctt
    22921 caactgaatt caatggaaat tacggccgag ttagaagaga tagttcaagc caacccgctt
    22981 cttgaaatga agagagagga ttatgagcta atagaaggaa aaagtgatct ggaaagctct
    23041 ccagaagacg aggaaaacac agaagatgga gatctctact cacttcccga aaacaactcg
    23101 atctacatga aacctgaaag tactgacagg gatggatggg acttcgacag gattgaagca
    23161 cagcaaccaa cgttcagaga aatactcaga gatttcgctt tctatattct tgacgacgat
    23221 cttttctccc tttttgaaat actccttgat tacatggacg atctaggaat gcttactgag
    23281 gatctggaaa agattgctcg cgagaatcac tgttccgtcg atgggctttc cagtgtcatc
    23341 tcgactatga gggacggggg attcgacggc atctttgcat cttcaaggga agagatagag
    23401 gccctattgg gtccgggtct cttcccttct tccggataca gtgacggcgc ttttgtaaga
    23461 tacgttgaac ctgatctcta tatagagttt atcgatgaga agttcattgt tacggtaagg
    23521 gactattcac ttgtactcag tgttgacgag tcgtatgagg agatcatgga acacggcgaa
    23581 aaaaaagcaa agaaatatct tgaagagaaa ctcgaggaag caaagttcta cataagcgct
    23641 ctcgaaagga gaaaggcaac gctgataaca attggcaacg aaatagtgat aaggaacgcg
    23701 ccatttctac ttaagaccag cgaaaagata aggccgttga agatgaattc agttgccgac
    23761 aagataggag ttgtagtttc aacggtaagc agagctgtga aggataagtt cgttcagact
    23821 cctgtaggga cttttccgat caggtacttc tttggaaatt tgcaggagaa ggaacaggct
    23881 ctcgaggtaa ttgccgaaat tgtcagaggc aacccagaaa tttcagatgc agagcttgtt
    23941 accgaactta acaacagaga tatcaacata gccagaagaa ctgtcaataa atatagaaat
    24001 cagttaggac taaggaagtc gaaatgaaga cgaaggccct gttgtttgtc ggtggacaat
    24061 acatatctac gatggagttc tacttcagga aactcaatga gacatcattt gtttcggccg
    24121 ccgattcagg ggcagaaatg cttaaaagcc tggggagaca tcccgatctc cttgtaggcg
    24181 atatggattc cgtaagccag gagaccctcc aatggtgcag aggaagagga tcgcttgtgt
    24241 taatatatcc ccccgagaag gatgacacag acacccaaat cgcgcttcaa gcccttgagg
    24301 agcgtggttt ctctaatgta gaaatctttg gtgcaacggg tctccggcta gatcatttca
    24361 tggggacctt agcgtctata tatggcctca gacacactct caaagccact attatcgaag
    24421 agaacgtcga aatcggtatg gtctctagag aaatgaccct ctttgtaaat cgaggtgaga
    24481 tctggtctct ctttccatat ggcggccagc agacaatagt ctctcttgaa ggtttcaaat
    24541 ttccactaac cgacgcatct ctagattatg gaaagccgct tggcgtttca aatgaaaccg
    24601 ttgatgaaga aatacagatt tcctgcaaag gaggcactgc cttatatttc agatggttga
    24661 aaaggtacta gtgctgtccg atactcacgg tgctttatcg cctttgacga gaatacttca
    24721 agaagtagat caaaatactt taatcatgca tagtggtgat ttcctatatc acggtccaag
    24781 gaatcctttg cctgaaggat acgatccaaa agggcttgca actcttctag caaagttcag
    24841 cggcagaatt gtaggcgtca gaggtaactg cgacagcgag atagacctcg aacttgtcgg
    24901 attggaaaat gcggcgagcg cactcacgtt gctgataaac gatttaggaa tcttcatttc
    24961 gcatggccac atgaaacacg aggtgccttt cgaacgcgga atagtaattt ccggtcatac
    25021 ccacatttcc ctcctcagaa aagataatgg gatcatcttc atgaatccgg gcagcctttc
    25081 tctgcctaaa gaccggaccg gtggctctta cgggacgatc gacttcaaaa aggggatcat
    25141 atctctcagg aacggcaatg gcttcgagct caagactttg gctctctagc ggcgcttgct
    25201 ttcgataaac atgtgactga acccatgata taataaatat ctgggtgatg aatatggttt
    25261 tgagatggca tggacattcg tgtttttcgc ttgagtgcga tggaaagact cttttgatcg
    25321 atcccttcga tgaaggtgtc ggttatgagt tgcccgaggt taagccggac attattctgg
    25381 aatctcatca gcaccacgac cacaatgcac acaaccgatt tgagtcaggg tacatacttg
    25441 tgaatcagcc ggtgagcgag aaagttcacg gtttcattat caaaggccat cccacatttc
    25501 atgatgatgt tgaaggaaag aaacgcggta agaatatcat cttcgaggtg acaactcccg
    25561 acggattcag agttgttcac tgtggcgatc ttggccaccg actgggccca gacttaacag
    25621 agacattgaa gagtcctgat attctccttg ttcctgtcgg tggagtgtat acgattgatg
    25681 cagggttagc tcgagagctt actagagatc tagctccttc ttacgtcgtg ccgatgcact
    25741 tcaagacaaa ggatctcact tttcagcttg cgggagttga agactttctt gccggagagc
    25801 cctttgaaca gtgtgaaaag ctggaactga aagaaaaggc gaatgtggga accagagtag
    25861 tagtcctcaa atatcgttga tcgagggcat aaaatggatg aaaagagagt tttatctctc
    25921 ctaggaatag gtagaagggc aaacaaggtt gtgtttggta aagaacaact aagaagttac
    25981 ttgaggcagc cattcaagag gaaatctttg attctggcta gcgataccag cgattcgata
    26041 aaggaagact gggtaaagag gtgcaagagc catggggcaa gctgtatcct cctcaaagaa
    26101 cacgaccgcg tcgctctggg aagggccatc ggcaaggaca acatatctgc agtggcaatt
    26161 gccgatgacg gccttgcaga cgaaatctca aagataatca cacaggcggg aggtgactga
    26221 gctatgccaa aagccagagt gtacgagctt gctaagagac ttaatataac ggcaagagag
    26281 ctgctggatg aacttgagga gctcggtgtt accgtcaaga accacatgtc agttcttgac
    26341 gaagagaccg tgaatattat cgtcggtctc tatgaggaag aaaagaaaga gactctgaga
    26401 aaggccgaaa aggcgaagag caagattcag gaaaaagagg caaaaaaact tgagaagaaa
    26461 ctggtctcca ttgaggagcc gccaaccgag aagaaaatac cggttgaaca taaagcaagg
    26521 acaatcaagc taaagagtaa tgagttcaag cttgatgtac tcgcgaagaa gatgaacgtt
    26581 ccgatttcta aggtgatcaa ggatcagttt atgaacggca taatacttcg tcccggtcag
    26641 atactttctc ttgaagacgc gatgaacatt gctaagaact acgaatggga agtggaactg
    26701 atccatgaac aggagattaa tccttttgat gctattgtaa gtgcgtactc tgatgcttat
    26761 aaggagagtt ccagacttgt tcagaggcca ccggttgtga ccgtaatggg gcacgtcgac
    26821 catggtaaga ccacccttct cgacagaata aggaaggcaa aagttgctga agacgagatc
    26881 ggtggaatta cacagacgat cggtgcctac caggtcgaaa taagaaataa gaagataaca
    26941 ttcattgaca cgccagggca cgaagccttt accgaaatga gagccagagg cgctcaggcg
    27001 acggatatcg tggttcttgt tgtcgcggcg gacgatggag ttatgccaca aaccatagag
    27061 gcatataacc acgccaagac agcgaacgtt cccataatag tcgcaataaa caagatcgat
    27121 aaaccgaacg ccaatgttga cctcacaaaa cagcagcttg cttcaaagct tggacttgtt
    27181 ccagaagact ggggaggaga tacagtaatg gttcccgtat ctgccaagac tgggaagggc
    27241 gtggaagatg tcctcgaaat gatactcctg gttgccgaag tagccgatat caaatgcatc
    27301 ccggaaggaa atgcaagagg tattataata gattccaggc tggacaaggc agtgggcccg
    27361 ctagctactc tggtgatcaa agatggggtg ctgaaaccgg gaagctatgt tgttgcgggc
    27421 tcggcttcgg gcagagtaaa agcgctgata aacgaatatg gaaagcgtgt taaggaggct
    27481 aatccatccg acccggtaca gatcattggt tttgatgagg tgccagatgt acatagcata
    27541 gtatacgcag ttgatgatct cgatacctcc aggtctctgg cggactttgc gaaaagtcaa
    27601 gcccagaaag agaaatctgt caccaccaaa cgccacgtca gactcgaaga gttcctgtca
    27661 atgaccggaa ccaccgatga gaaaaaggaa cttaagctta tattgaaggc agattctttt
    27721 ggtactgtcg aagccctgaa acaggcaatg gctaagcttg agaacagtga cgtgaagatt
    27781 gaagtagttc attccggtat aggttcagtg aattcgagtg acgtgatgct tgcatctgca
    27841 tccgacgcag ttataatggg gtttaaggta aaacccgatg caatggcgag aaagcaggcc
    27901 gaagttgaag gagttcagat aaaggtctac gagataatct tcaatctgat agacgatttg
    27961 aagaaagctc ttgaggggct tttagagccg gaagaggtcg atgaagtaat cggtcatggc
    28021 catataaagc agcttttcaa aattagtaag gttgggacca ttgccggtgt acagatcaac
    28081 gacggatatg tattgaaaaa gggcaagatg agagtttaca gaagaaacga ggaagtggca
    28141 gaccttgcaa tagaagtctt aaaacactac aaggatgatg tgagccgagt cgacgcccca
    28201 aaagagtgcg gaatcaaggc ggtaggcttc gatgatttcc aggaagaaga tcagttagag
    28261 tttcacagca aaaagagcgt aaagagaaca atagatttca ccgaataagt ccccgttaaa
    28321 tgaaagaggt ccctttatgg ggcctttttt tgcagatgag aggcctggac aaagtgacgt
    28381 tggattcgga agagaaaact cttatctcca gagcgtgtct ggggacccga gtctgagttt
    28441 ggcgaacaga agcgctgcac cattcatgat tcccagttct accggggacg tcgaacatct
    28501 gcaacttccc ataactcatt cactatgtca tctgatttat tccaggatct ggagttgtaa
    28561 gaccgtggtt gggggttcgt aagagcaaga gagactggtt gaccgttgag aggttctccg
    28621 tt
//
LOCUS       DAOQYI010000024        27956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_1980_length_27956_cov_73.105516, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000024 DAOQYI010000000
VERSION     DAOQYI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 27956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27956
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1980_length_27956_cov_73.105516"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(529..1152)
                     /gene="plsY"
                     /locus_tag="PKL93_07310"
     CDS             complement(529..1152)
                     /gene="plsY"
                     /locus_tag="PKL93_07310"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNQ71101.1"
                     /translation="MRYLLFALVGYVMGSIPFSFIVPLVKGVNITKVGSGNVGGTNVL
                     RSMGGYAGAVAMILDGVKAFLPVLIARAVFGVSLPEGMMIGFFAAVGHSYSIFLKFRG
                     GKAVACTVGTLSGTLPWYVAVFFGIWIPIVLVTQYVSLGSLLALAILSGILFLTEGYA
                     VGLWMLAFFGLSLFRHRKNVVALFRGEERKTDLIAAFKRKRNEIKNR"
     gene            complement(1149..2474)
                     /gene="der"
                     /locus_tag="PKL93_07315"
     CDS             complement(1149..2474)
                     /gene="der"
                     /locus_tag="PKL93_07315"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNQ71102.1"
                     /translation="MATVLIIGRPNVGKSTLFNRLVGGRRAIVDDLPGVTRDFTVGRV
                     NWQQRTFQLVDTCGLFENPENIVEEKMKEVTLELLSEGDLILFVVDGRFGLTSADQVL
                     ADYLRKQRIEVIFVANKVENPESFYSNMGNEIFSLGFGDPIKISAAHGLNIDLLLDEI
                     FERLETLGHSVHLESNDESELKIAIVGKPNAGKSSIFNWIVGSPRSLVTEVSGTTRDT
                     VDETVEVDGIPITFIDTAGIRKKSKVKVMNVEYYSVMRAIDALERSDIIVMVIDSADG
                     VGNQDQRIAGLAEKNGKATIAVFNKCDLVDERRRKALLRTFKQDLYFIDYSPVIFTST
                     LTGEGMDELIDSIKLVSKKIDLRLPTGLLNTILQRYSEGTPAPGKGKKRGKIYYGSQI
                     ASRPPIIMLNVNDPSLFSEPYLRGIRNTIRLNFDPFEGTPIFLKLRRKR"
     gene            complement(2474..4189)
                     /locus_tag="PKL93_07320"
     CDS             complement(2474..4189)
                     /locus_tag="PKL93_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNQ71103.1"
                     /translation="MEEKMNRQDENLSMEEIFEQMDVSSARKGSRKDAEVFSIQPDGL
                     MVIMDGKLDGFVPADQLVNELEEYNVGDKIEVMVIKTNEEEGRSTVSEKRVHARQAVS
                     KVEKAYREGKAIDGKIVSETKAGYNVRLVKTVPAFLPGSESGIRKGDPAPEGTLKFKV
                     IRFKRQRNGINVVVSLRAFQEEAIKAYFETIEVGQELEGTVESIKNFGAFVKLNDNVT
                     ALIPASEMSWDPSVRISDLLKPGQTVRTKVIGVDEAEKKISLSLKQMTEDPWSTLKDR
                     YEFDSTVVGTVKNITPFGFFVSLEPGVEGLVHISEVFWGNIKKDLKSVVEVGDEVKVT
                     IKEINEEKRTLSLSYREAMGDPWQNIGDKYKEGDILKAKTAKVLPTGVILELEEYVSG
                     FVPVSEVSWNFVDRIEDVVKEGDEIEVKILSIDSVNRRMRLSIKQAYSDPWEKVSAEL
                     SVGDHVTGTVTRLTKSGAIVLIDSYGVEAFLPVSQVSLERTENIEDSVQVGRHDQFKI
                     IRLVYDPDNDTRNMVVSIRQLIKDKEAAETDKALKDLNGNGSVNSNSLGEMIKNQLEE
                     EGESL"
     gene            complement(4161..5684)
                     /gene="cmk"
                     /locus_tag="PKL93_07325"
     CDS             complement(4161..5684)
                     /gene="cmk"
                     /locus_tag="PKL93_07325"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNQ71104.1"
                     /translation="MRIAIDGPAASGKSTVARLVAEKLDFVYIDTGAMYRALALKAKK
                     AGISFSNADEMAELMSHTYFSLSDSGIILDGSPLGDEIRTREVSLLSSDIAKYSYVRD
                     FLTEQQRTLSKQGNVVMEGRDIGTVVIPEAELKIFLTASAGERARRRVKQLKSSGVEA
                     TYDEILAEIERRDLNDSTRSVAPLKAASDAIILDTTGMSIEEVVSRIVSLAEKRQRVH
                     LARSVGFCYGVDRAVSEAVKLLKSGKKVYATGEIVHNEKVMDDLKRLGLEVIDDCILS
                     GRHEGVAIIRAHGIDPASEEKLRRVFDEVIDLTCPIVYNVFSLAVDLQRQGSFIVVYG
                     KKNHAEVTALSGRLNRYLIVEPGENYEKVLKKLEGLDQIAIVSQTTMSSLDFENFSGF
                     VKSHLGERVAVYNTICKVTIQRESEAERLAKISDTVIVIGGKNSSNTKKLVKIVQRLG
                     KRALHVTDLTDLPQGDMGKVAIISGTSTPYEQIQKILDYIDNKREVTNNGRENESAR"
     gene            complement(5687..7462)
                     /gene="aspS"
                     /locus_tag="PKL93_07330"
     CDS             complement(5687..7462)
                     /gene="aspS"
                     /locus_tag="PKL93_07330"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNQ71105.1"
                     /translation="MLELKKRTHTCGELRASDTGKVVTLNGWVDRIRDLGGIKFVQVR
                     DRYGFTQVVFDPQDNELYNMALKLGNEFCISVTGSVRKRPDDAINLRLPTGEIEIVAS
                     EMGILSDAEVPPIYINIDEESAEEMHLRYRYLDLRRPKMQRNILTRHRFVQAVREYLN
                     NNGFVDVETPILGKSTPEGARDFLVPSRLKAGKFYALPQSPQIFKQLLMVSGFDRYYQ
                     IAKCFRDEDFRADRQPEFTQIDLEMSFADEEDVFSMTEGLMKYAFEKAASADISTPFR
                     RFTYQEVVEFYGSDKPDTRYGMKIHDITSIFEKTAFKVISDSIDKGEKVKGFIVEGKA
                     DRFSRKKLDEYTAFAKENGLGGLMWLKVEGDGLKSSILKHCPEELSSLVSLFAANEND
                     LILFSLGEGLSLSKALGQMRNKIIKEEEFSVEGFEFLWVTDFPMFSYNEEEQRIEAEH
                     HPFTMPDLEDLEKYGESDPLKVRSKAYDLVLNGYEIASGSVRIHRRDIQSRVFDLIGL
                     SREEATEKFGYLLEAFKYGPPPHAGIAPGLDRIIAIIVGAQSIREVIAFPKVASGADL
                     MTSAPSEVSQKQLDLLKLQLKDTEN"
     gene            7565..9298
                     /locus_tag="PKL93_07335"
     CDS             7565..9298
                     /locus_tag="PKL93_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNQ71106.1"
                     /translation="MKVLLASLLILGLAFTSAFAAVNYVDVSSNHWAYDAVMKLSDLG
                     ILTGIVQADGKTYFNGNDALTRYQTAVMLKKTLDYVELNFARQGTVPQNGAVDGTVMS
                     RLEALELALTDSTGRLIDTMDLQLRITALENRISSLGISTNGSVSQSTVESLRQQIMK
                     FVEDLSLTTKKLDMLALDVQNVQNSMSDLTVMESKVNDAVRRVDTLSGNVSMIQSSIS
                     SNERAISTLDSTVRSLSSSLSDYDAKLSTIINRASANAIEIASIKESMTTLSGDVLSV
                     RDLQNKISSLEDQIANVKLPTEAIDKLDYLSARVNAISSDYAKIGDLQNYVTKTDLKA
                     NLNLYAGIDELAQVKNSSEVLYKDLQTLRDDFASETGIIKSDIGNLQRSINAINEIVT
                     IHENELASLKSSVSTVSTLRSDLNALNSKFNALTDQQSKNFAATQASIGDLEARINEN
                     MDMLNVTVDASLSRISLGMDTVNTQLGKNESEIKALKTRADSLESRLNTTVVNLERYL
                     KISDLETQPVIVNLSERVAEINKATVDAATKQDVEALQKKTSPWLILSTVSSLAALGI
                     SIWVLIDSGII"
     gene            9386..10684
                     /gene="asnS"
                     /locus_tag="PKL93_07340"
     CDS             9386..10684
                     /gene="asnS"
                     /locus_tag="PKL93_07340"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNQ71107.1"
                     /translation="MKVAEISSLGKHINEHVQIRGWLRRKRSSGKIQFLFLRDGSGFV
                     QAIVEKSSVPESVFQVAASLKMESSLIVTGLVKAEERAPGGVEVLVEDVQIVQIPEKD
                     FPINKPDHSIDFLMDNRHLWLRTQRQTHVLKIRHEIIRAVREFYNDRGFVLVDTPIFT
                     GSIGESAGNTFEIDYFDYGKAYLAQTGQLYLEAAAMALGKVYNLGPTFRAEKSKTRRH
                     LIEFWMNEAEVAYYGHDDNIKLQEDLVTYVVKKTLERAGEYLRSVGRDTSRLEKIETP
                     FPRITYDEAIKLLVKKGFDIKWGDDIGGDEETAIANEFEKPVVVERYPRMMKAFYMQP
                     DPERPEVVLCDDMLAPEGYGEIIGASERIHDRDLLVERIQEYGLDVESYDWYLDLRDY
                     GTVPHSGFGMGIERIVAWIAGLEHIREAIPFARTLYRIHP"
     gene            10693..11742
                     /gene="ruvB"
                     /locus_tag="PKL93_07345"
     CDS             10693..11742
                     /gene="ruvB"
                     /locus_tag="PKL93_07345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNQ71108.1"
                     /translation="MREERFLTPGEVQEDNSIKSLRPKTLQEYIGQNQIRQRLRVAIE
                     AAKVRREALDHTLLAGPPGLGKTTLAHIIANELGSEIYVTSGPVIEKQGDLAAILTGL
                     ERDDVLFIDEIHRLNRSVEEILYSAMEDFQLDIMIGKGPSARSIRLDLNPFTLVGATT
                     RSGFIGAPLRNRFGMILEMEFYPDRDLKQIILRSATLLGTSIRDDAALLLARRSRGTP
                     RIANRLLRRVRDIVTVEGESEITMETVESAMTLLSIDSEGLDSMDKKLLSILIESYNG
                     GPAGVKAIAASLGIEPETISEVYEPFLLQSGFLVRTSRGRMVTEKAYRHLGLPLEGRN
                     TPLWNYADHREEVEE"
     gene            11890..12708
                     /locus_tag="PKL93_07350"
     CDS             11890..12708
                     /locus_tag="PKL93_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNQ71109.1"
                     /translation="MKAAVFYNRTRISAEMLDWLSREIELHKIDTSFCEDRASCSVPE
                     QDVILTFGGDGTVLEAVQMAVLNNLPIMSFRVGSVGFLAAFELSMLHTALDLLQEGKL
                     DGVDRNVMEISLDGTVRYALNDCVVERSTPSRTVSLSVEISGQSSYQVVGDGIIFATN
                     TGSTAYTMAAGGALVDPEANCFQITPICPHNPFVGSIVIGASRKVRMEVKQDKGFPLE
                     AYVDGELVSELRTGETIEVSLSDRKVTLLREGSFDFVALLKRRLAFGGRLKDDI"
     gene            12698..13399
                     /locus_tag="PKL93_07355"
     CDS             12698..13399
                     /locus_tag="PKL93_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HNQ71110.1"
                     /translation="MISKVGLERIGNLRIEILRMARYVQSIFNKTMVAVLERNEHQAR
                     DVIYSDSVIDYHQVELQAMIIGLTGILTPTGKELRLLTLSMNIVSALERIGDKCVFIC
                     ETSLSLSKRPPIGYYDKMKSMFESVSIMTKGAIDNLVEPSLSEAERLCKSDDAIDRMQ
                     EEVKSDLIRYCEQSPSVVGRAFDLMMIFCALEEIADLSTELMEAAVYIETGRHYRCVN
                     DDFKPVDFETQNEEG"
     gene            13406..17758
                     /locus_tag="PKL93_07360"
     CDS             13406..17758
                     /locus_tag="PKL93_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNQ71111.1"
                     /translation="MKTLIKYIGAQALGSIFVGLAGFIIFVSLELLYYLSDMIIRYKV
                     GIDKLFLLIYYNLPEFIVLGIPVGILLAIFWVLSRMRSDNELIALQTHGISLKRLVIP
                     FLLIGIIFSGFAYLLNDYLVPAASTKANEAMARFVYKQPEVTLKENVFMQDSQGRMIY
                     VRRIDQETKELKNVSIYEVSRGQVTLTTAESAVISSNKWILSKANIYQTDESGFLGVE
                     MQFGTAEFEIDEDIERYLASFKSPKEKTSKELRDDIEAFKNTGINTSSLEVALQEKYS
                     MSIAPLVIVLLGVPVSLMFNLQSKSWSVILTFLLVVIYQGSGAWLSGMGKENLINPSL
                     APWIPNITFSILGVIIYSLIDTKASYRLSELFTRIMKVSAIVLLLLVPGLLEGSNVKI
                     VGGTIAGTKSGREILLGGGVKVEYFSETMNATIEASNASILNVDSTPESISFWGNVKM
                     LSDETTILSDRLILDLTNERVESMQVYSRTELEVPAARGDTSKTGSKVPFYVFGDYVQ
                     STMEASPTFNIGEGYVTTCDKTHPHYRFRVSSAVVTPGKGMSVQNMLMYIGNVPVFYL
                     PAYYFPLDDPERRPFDVDLSGITEAKTRITLRYLELDWLLLQGTWYRDWISTEDAFTA
                     KSVLYTPLGDLELFGQFGQQKETSYGAYVLLKPAFDWLRIQGGALYLSGPSLKELQTP
                     FSGINLGETISKNKVTQLDPFAVKESMNATEMQLAYVQLLSPQDWELELDVIGAFARY
                     DDENLWMVNLQNLTVPSDGSIDAGFFKLSTSEFNVNGLFGQRYVNNKVLYSLRTKASV
                     LKTESSLFGADASVDSLNFTLASNAATVTELFDPSNMEDFVLEVNKYLFTAGNLKIGG
                     DIKSTFDASTTELNIIMPSDNKGIGRNYLTYASDDGKLKVNGRYALDFDSLHEKNKDK
                     LFWIDPFDILYDGFVSVELKFYFESRYDKSFKIEGKKLRVYKDIELYKGEWGLLSVDV
                     ILEPAYEAGFIYDSEEGWELTKNEIPVTLKNEVTFRPFDWYYVGLQYNPVLTYDFINR
                     EWRLDHPGKLLTGIDTEIFKADYALELDYEELVASPTEMNWLGKGILTTEAEFALWEV
                     TLGQKTETVFMPVTSQASETSYSVTFDSEVFSHSTKSKVYWQTGDKLDDFVNKERLTI
                     KVATETSFEFTLDWTFDASPSVADDERMLKDLVFGSSIRVKDFAELGVKFRYPYLFGS
                     RKIYDRLKIEVNDLSIGDVSWKNASIDFTTGFSEFDSKYKYPEKYFTKSETQAQFLLW
                     QSTRLSVSSLSVGEYFIATSTASETGDTAYSFGISNVKLGNKPLLGGTNAKFDEFGLS
                     MELRKSEDLYFTMHINELYFPMGSISDTPGVLNRLSLGIDGSGKRSFVEIGTYAGDIS
                     MWDESIAKVSPMYFDLHCMALELILRLSFGSDVTTIQDTVESIALKYYIKELKDRYFV
                     IGLYKGAPFFRFRF"
     gene            17770..18387
                     /locus_tag="PKL93_07365"
     CDS             17770..18387
                     /locus_tag="PKL93_07365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNQ71112.1"
                     /translation="MTDYVKRKELLLELNSKISDCSLCPLSSSRTNTVPGEGSIETPV
                     VFVGEGPGADEDASGRPFVGRAGELLTKILESVKLSREDIFITNIVKCRPPKNRVPTA
                     EEQRACSPYMLSQLAIIRPKLIVALGATALSYFVNEEGIQITKVRGRIFDWIGGIKVF
                     AMFHPSYLLRNASRAPGSPKSLTWEDIQNVRKMYDQLLSGRNIDI"
     gene            18397..19341
                     /locus_tag="PKL93_07370"
     CDS             18397..19341
                     /locus_tag="PKL93_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ71113.1"
                     /translation="MAENYLVTGGAGFIGSHVVDHLISSGKTPVVVDNLSSGKIENLD
                     PRALFYEQDITDQEMMERVFMLHRPTVLFHLAAQISVSRSVREPEEDAMVNIIGSLRL
                     LKIAAKYGLKKVIFSSTGGAIYGDDVKRIPTDEEELPKPLSPYGIAKYATENYLRFFS
                     SELGIKYTVLRYANVYGPRQDPYGEAGVVAIFSERMLHNQEVVIFGDGECVRDYVYVG
                     DVARANLLAIEKCENTVINIGTGIGTSVNELFDIMKPIAGYSREAVHKEPRPGDVKKS
                     ILNIERARSLLGWEPSTQLERGIKETIEYFRSGLDSSQ"
     gene            19304..20617
                     /locus_tag="PKL93_07375"
     CDS             19304..20617
                     /locus_tag="PKL93_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNQ71114.1"
                     /translation="MSISEADWTPLSERLRPKEFDDIVGQEHLTGKKGIIRGAVDSGK
                     LFSMILYGPPGTGKTTIGEIIRSKLGNDYHFEFFSASLQGTADLKKHFAHGERLKRVG
                     QQLVLFVDEIHRLNKSQQDVFLPVTEKGIIILIGATTENPSFEVNPALLSRCRLVILK
                     GLKDSDISALLNKALEKDLILKESGVTVDGEVIEVIAQSSGGDARIALNLLDTLVESA
                     AALDKPVLDLEVMNELSVLPSGRYTKKGEEHYDIVSAFIKSLRGSDPDAALYYMLRML
                     EGGEDPKFIARRMVIFSSEDIGLSDPMALVIANAAFQAVNNVGLPECILNLSEAAIYL
                     SVATKSNRTYSAMVKAQEKVKNTPNLEIPLKLRNPVTKMMKELGYGKGYNYPHNSGGF
                     SREYYLPEPIKEEVFYVPGNSGKEKFLFERLRSLWKGMKNYPEEE"
     gene            20614..21174
                     /locus_tag="PKL93_07380"
     CDS             20614..21174
                     /locus_tag="PKL93_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNQ71115.1"
                     /translation="MKSFILREGRVIQLEISDITEQEVDVIVNAANSYLRHGGGVAGA
                     LVRAGGKEIQLESDAYVRKNGPLKTSEVAVTSAGNLLAKKIIHVFGPQYGELDLESKL
                     YCSFANVLKKAAEMTAVTLATPAISTGIFGVPVSICAEQFLKSVMDHFSQNKSSSLRL
                     IKMCLLDRKAYDEFLSIALDFFSSIE"
     gene            complement(21158..21832)
                     /locus_tag="PKL93_07385"
     CDS             complement(21158..21832)
                     /locus_tag="PKL93_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HNQ71116.1"
                     /translation="MKKVVFLLVLLLPGIIFSYVGMLDDFDTALKLAKIEDKEAIVMF
                     SDTSCVYCVKFVKETLADETVQDLLKAGFVFSQIYKSNPGKASYVVGEEWKEMSYGEL
                     YAYFQIRGTPTFWFFTSENALLTNLPGYVPAKDFTTILRFLGERAYETATFEDYRSKE
                     STFMGEPKIVRVSEEDYNYVLQNDPIAREYKDQEVDKFTVWLTKDESTAEYLLEEGVF
                     RVILLN"
     gene            complement(21836..22570)
                     /locus_tag="PKL93_07390"
     CDS             complement(21836..22570)
                     /locus_tag="PKL93_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HNQ71117.1"
                     /translation="MNELTALATGTLGVSPMISPIVTTSVTYGSAFLGGLLSFFSPCI
                     LPLIPVFFGVLMGGAADAKARFIRGVFFTLGMSIFFFILGIGATGLGTFIRQNEVLMN
                     IISGGLFVLFAFLYLFEIGMKGVNLNVWKFGGSAVSGFILGAVLGLVWVPCAGPILGS
                     ILVLAANQSAVAEGGLMLLVYSLGLSIPFLTLSGLVAKLTSKLSFGGESKWRKVIRVS
                     VFVVLLAAGLLTMTGNLNLLQFATGG"
     gene            23319..24179
                     /locus_tag="PKL93_07395"
     CDS             23319..24179
                     /locus_tag="PKL93_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HNQ71118.1"
                     /translation="MEGYVTVPGGRIWYEVQGRFGKMKPIITIHGGPGVPHNYLETIS
                     ALSDERPVVFYDQLGCGRSERPSDRSLWRVDRFVEEIECLRKELGFSTINLLGQSWGT
                     ILACEYSVKYPSFAESLVLSGPAMSIKRFESDTRKLVETLSDHSQSAIKRAESIGNFN
                     GESYQSAISEFYGKYVCRMEPWPQCMNEAVAGMGEEVYNYMYGPSEFTVTGVLKDFDC
                     TPHLENITVPVLLTCGEFDEATPETTRYYSSKFPNATMKIFEGASHEHHLEKAKEYIE
                     AVRRFLKDVE"
     gene            complement(24242..25972)
                     /locus_tag="PKL93_07400"
     CDS             complement(24242..25972)
                     /locus_tag="PKL93_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ71119.1"
                     /translation="MRKLLALILTVSVLTMAMASHFHLTILHTSFAPANTAPFDYSSG
                     KSEDVFLARLAAFVNQMRLFNPNTLFIDTGNLIRGTPLSYYSAKIDYCNLNPFVETMN
                     AIGLNASLIGSEEFIYGPGFLEEAISASEFPLLSANIVFKSSGEPVFSPFEVVEIEDD
                     GEKLRVGIVGLVTEVIPPWQEAEFFAGLSFVDSVDAAGDYLGKLREEVDVLVLAYRGS
                     NEEAHEIMKALREIDVFLKGPQLTTKSAIVDGVVISGPGYIDESVSKIDIYLEKTGEG
                     WKIYDRSVDEISMSGFLPDESVVEIFRGYNDQIQKLLDTRVGFAVGDFYVEDALEARL
                     RDNPLAEFINRVQMEVTGAKISCTSIFDHATTGWKTGPITIGEIYEVYPVPSTLRVLE
                     LTGGDIKEALEFNAERFVTDNGLLTLDHWSVDDYSNYDMWEGIEYIIAIDRPVGERVL
                     SVWHNGKPLDMAETYEVSMSSFRAGGGGYSMFENKPVVRDVKLQIFEIITDFVQERFA
                     ISPQVDNNWSVGTGFIHTVGWNETLRSISEMYDIEDSEIMKYNPDLVGVKSIPAGSEL
                     IVYRPFFQDK"
     gene            complement(26050..26535)
                     /locus_tag="PKL93_07405"
     CDS             complement(26050..26535)
                     /locus_tag="PKL93_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNQ71120.1"
                     /translation="MITARMEKEINNQIKAEFESAFIYMSMAAWSHKNGYFGAENFMW
                     KQAKEEEEHAMKFIQYLKDVDATAEIPGIEKPNGTYESLLDVFEKGLEHEKYITSRIH
                     NLVKISEEEGDYATNDFIQWYVTEQVEEERNFRDVVKRLKMVGNQANGIFMIDSQLGE
                     R"
     gene            26774..27832
                     /locus_tag="PKL93_07410"
     CDS             26774..27832
                     /locus_tag="PKL93_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ71121.1"
                     /translation="MTVRKIFLFFSMILIAAALLAAPLKVAFVYVAPVGDGGWSTMHN
                     EGRLHLERVFGDQIVTDFIESVPEGAEAEVVFRGYAQRGYDLIFGTSFGYMDSMVKVS
                     QAFPDSTFMHCSGYMTTDNLGTYFGRMYEPRFLSGLIAGAMTKTNIIGYVAAYPIPEV
                     IRGINAFAMGVQYVNPEAKVHVVWSNTWFDPAREKEAAESLLDIGADLITQHQDSPAA
                     QQAAEDRGVFSIGYNSDMSAFAPNAFLAAPVWDWGPFYEYVVRNVMDGTWTNEQYWGG
                     IKEGVVDIFVSDLVPDGLTKLVMTMREAIVQGLFHPFDGPVYDQSGALRYAEGEKPTD
                     AELLSMDWFVNNVVGKIQ"
BASE COUNT         7637 a         6406 c         6407 g         7506 t
ORIGIN      
        1 gtcctccgac aagaggaccg gttcttcgtt ccgacgctac gcgtccaggt tcatggaaaa
       61 aagatgtgag agggcagagg taagaggctt gaagacatga gatcccgtgc agtttgcctg
      121 aacagagacc ccaaacagga gcatattggg gcaagcttca gggcaggctc cttggaatgg
      181 caacgaatgc ttgttccgaa agcgtctaaa tccgtcagct gaacttgttt cagcatctag
      241 gtcatggttt tttgacctta ttcaagaacg aaaaacaaat cctcgctctg gaaccaagga
      301 taagctctct tcgacaaacg aagaaccgcc cttaatgctc ttccaaaggg cgggtcattc
      361 tctgggatgg acgaaaacgg tttttcagca gcgatctgca atccacagca atcgccttgc
      421 ggatttttac tacgaagtcg gaactggcct ttacgaagca aggactggtc gcacttagcg
      481 cgactggcga tcacgagcag cttttcacag atgcatccag gaaactgcct aacgattttt
      541 tatttcgttg cgtttccgct tgaaggcagc gatgagatcg gtttttcttt cctcacctct
      601 aaagagggcc acaacgtttt ttctgtgtct gaaaagtgag agtccgaaga aggctagcat
      661 ccataggcca accgcatacc cctctgtgag aaagagtatt ccggagagga tagccagagc
      721 caagagtgat ccgagagaaa cgtattgggt aacgagaaca atgggaatcc agatgccaaa
      781 aaagactgcc acgtaccacg gaagggttcc ggaaagcgtt cccacggtac aggccacagc
      841 cttaccgcct ctaaacttca gaaatatcga gtagctgtga ccaactgctg cgaagaaacc
      901 tatcatcatt ccttctggca gagagactcc aaagaccgcc cgagctatca gtacgggaag
      961 aaaagccttc actccatcga gaatcattgc gacggccccg gcatacccgc ccatgctcct
     1021 tagtacattg gttccaccta cgtttccgct tccaaccttt gtgatgttca cacctttgac
     1081 aagtggaaca atgaaactga atggaataga gcccatgaca tatccgacca aggcaaatag
     1141 aagatacctc atctcttcct cctgagtttc aagaagatcg gagtcccttc gaatggatcg
     1201 aaattcaaac gaatcgtatt ccttatacct cgcagataag gttcgctgaa aagtgaagga
     1261 tcgttcacat ttagcatgat tattgggggt ctactggcga tctgcgatcc gtaataaatc
     1321 ttgccccgtt tttttccctt acctggggca ggggtccctt cagagtatct ttgaagaatg
     1381 gtgttaagaa gtccagtcgg caatctcagg tcgatcttct tgcttaccag ctttatgctg
     1441 tcgattagct cgtccattcc ttcgccggtc agtgtagagg taaagatcac cgggctgtaa
     1501 tctatgaaat agagatcctg cttgaaggtt cttagtagtg cctttcttct tctttcatct
     1561 acaagatcac acttgttgaa aaccgcaatt gtcgccttcc cattcttttc tgccaggcca
     1621 gcgattcgct gatcctgatt tccaaccccg tccgccgaat cgatcaccat cactatgata
     1681 tccgatctct cgagtgcatc aatagccctc atgacactgt agtactctac gttcataact
     1741 tttactttgg acttcttcct aattcctgcg gtgtctataa atgtgattgg aatgccatca
     1801 acctctaccg tttcgtcaac agtatcacga gttgtgccgg atacttcagt aactagactt
     1861 cttgggcttc cgacaatcca gttgaagatc gaggattttc cagcgttggg tttcccaaca
     1921 atcgcaatct tgagctcaga ttcgtcattg ctttctaaat ggacagagtg gcccaatgtt
     1981 tccaacctct caaaaatctc gtcgagaagt aaatcgatat tgaggccgtg agcggcagaa
     2041 atcttgatcg gatctccgaa accaagtgaa aagatctcgt ttcccatatt tgaatagaaa
     2101 gactctggat tctccacctt atttgcaacg aagattactt caattctctg ctttctcaag
     2161 tagtccgcca gcacttgatc cgcactagtg aggccaaatc ttccatcaac gacgaacaga
     2221 ataaggtcac cctcgctcag tagttcgagg gtgacctctt tcatcttttc ttcaactata
     2281 ttctcgggat tttcgaaaag gccacaggta tcgacaagct gaaatgttcg ctgctgccag
     2341 ttgactctac ccactgtgaa atcacgcgtc acaccgggaa gatcatcaac tatggccctt
     2401 ctaccaccaa caagtctatt gaagagagtt gactttccaa catttggtcg tccaattatc
     2461 aggacggtag ccactagagg ctttctcctt cttcctcgag ctggttcttt atcatttctc
     2521 ccaaactgtt ggagttaacc gatccatttc cgttcagatc cttgagggcc ttatcagtct
     2581 cggcagcctc tttgtccttt ataagctgtc ttatggaaac taccatgttt cttgtgtcgt
     2641 tgtcgggatc gtagacaagc ctgattattt tgaactggtc gtgtctacca acctgtacag
     2701 aatcttcgat attctctgtc ctttctagag aaacctgaga gacaggaagg aaggcttcaa
     2761 ctccgtaaga gtcaatcaag actatcgcgc cggacttcgt gagccttgtg acagttccag
     2821 tcacgtggtc accgaccgat agttctgccg aaaccttttc ccaggggtcg gaataggcct
     2881 gttttatact cagtctcatt cttctgttta cggagtctat tgaaaggatc ttgacttcta
     2941 tctcgtctcc ttctttcaca acatcttcga ttctgtcgac aaagttccag gagacttcgg
     3001 aaaccggcac aaatcctgaa acatactctt caagctcgag aatgacgccg gtaggaagta
     3061 cctttgcggt cttcgccttg aggatgtcac cttccttgta cttgtcgcct atgttctgcc
     3121 acggatctcc catagcttct ctgtaagaaa gagagagagt tctcttttcc tcattgattt
     3181 ccttgatggt tactttgact tcgtctccca cttccacaac gctcttgaga tccttcttga
     3241 tgtttcccca gaagacttct gagatgtgaa ccaaaccctc tactccgggt tcgagcgaaa
     3301 caaagaatcc gaatggagtg atattcttaa cggttcccac tactgtagag tcaaactcat
     3361 atctgtcttt cagtgtggac caggggtctt cagtcatctg cttgagagaa agggagattt
     3421 tcttctcagc ttcatcgacc ccgattactt tggtcctgac tgtctgccca ggtttgagaa
     3481 gatcgctgat tcttacactg ggatcccaac tcatctctga tgctggaata agagcggtca
     3541 cgttgtcgtt gagcttgaca aaggcgccga agttcttgat agattcaacc gttccttcaa
     3601 gctcctgacc gacttcgatc gtctcgaagt aagccttgat agcttcttcc tggaatgccc
     3661 ttagagaaac aacaacgttt atgccgttcc tctgtctctt gaaccttatt accttgaatt
     3721 tcaacgttcc ctccggggcg ggatctccct ttctgatacc cgactcggat ccgggtagga
     3781 aggccgggac cgtcttaact aatctgacgt tgtatcctgc ctttgtttct gaaacgatct
     3841 ttccgtcaat cgcttttcct tctctgtagg ccttctcaac cttgcttact gcctgtctag
     3901 catggaccct cttctcggag acagtacttc ttccctcttc ttcgttagtc ttaatgacca
     3961 tgacttctat cttatctcca acgttgtact cttccagttc atttaccagc tgatccgcgg
     4021 gtacgaaccc gtctagcttt ccgtccatga tcaccatcaa gccgtctggc tgaatggaga
     4081 aaacctctgc atcctttcta cttcccttcc ttgctgagga aacatccatc tgttcaaata
     4141 tctcctccat gctcaggttt tcatcttgcc gattcatttt ctcttccatt gttggttacc
     4201 tccctcttat tgtctatgta atccagtatc ttctggattt gctcgtacgg agttgatgtg
     4261 ccgctaatta tggcaacctt ccccatatcg ccttgaggaa gatcagtcag gtctgttaca
     4321 tgtagtgccc tcttaccgag tctttggact atttttacca gtttctttgt gtttgagctg
     4381 ttttttccac ctatcacaat taccgtatcg gaaatctttg ccagacgttc ggcttcagat
     4441 tcacgctgta ttgtaacctt gcaaatcgta ttataaacag cgacacgttc tccaagatgc
     4501 gatttcacga accctgagaa gttctcaaaa tccagactgc tcattgtcgt ttgagagaca
     4561 attgcaattt ggtcaagtcc ttctagtttt tttagaactt tctcgtagtt ctctccgggt
     4621 tcgactatga gatacctatt cagtctacct gaaagagcag ttacttcggc atggttcttc
     4681 tttccatata ccacaataaa actgccttgc cgctgaagat ctacagccag gctaaataca
     4741 ttgtacacta tagggcaggt cagatctatt acttcatcga aaacacgccg taatttctct
     4801 tccgaagcag ggtcgattcc atgagccctg attattgcga caccttcatg tcttccagat
     4861 aaaatgcagt catctattac ttctagaccc agccgcttga gatcgtccat cactttctcg
     4921 ttatgaacga tttctcctgt tgcatatacc ttctttcccg acttaagcag tttcacagcc
     4981 tcactgacag ccctgtcgac tccgtagcag aagcctactg accttgcaag gtggactctc
     5041 tgtcgcttct ccgccagtga tacgattctt gaaacaactt cttcaatact catacctgta
     5101 gtgtccagaa ttatggcgtc cgaggccgcc tttagtgggg caacggatct cgtcgagtcg
     5161 ttaagatccc ttctttctat ctctgcgaga atttcgtcat aagtagcttc taccccactg
     5221 gatttcagct gcttaactct ccttctggct ctttcgccgg cagatgcagt aagaaatatt
     5281 ttaagctcag cttcgggaat aacaacagta ccgatgtctc gcccttccat aacaacgttt
     5341 ccctgctttg aaagcgtcct ctgctgctca gtcaaaaaat ctcttacata agaatacttt
     5401 gcgatatctg aggaaaggag cgaaacctct ctggttctta tctcgtcacc aagagggctt
     5461 ccgtctagga tgataccgga atccgaaagc gagaaatacg tatggctcat cagttcagcc
     5521 atttcatcgg cgttggaaaa ggaaattccg gctttcttgg ccttcagagc gagtgctctg
     5581 tacattgcac ccgtgtcaat ataaacaaaa tccagtttct cagcaaccag ccttgcaaca
     5641 gtagattttc ccgatgccgc cggcccgtcg atggcgatcc tcaacttcag ttctccgtat
     5701 ctttcagctg tagtttaagc aaatcaagtt gcttctgcga aacctcggaa ggtgcggagg
     5761 tcatcagatc tgcgccgctg gcgaccttcg gaaatgctat tacttccctt atagactgag
     5821 cgccaacgat gattgcgatg attctatcta gaccgggagc aattcctgcg tgaggaggtg
     5881 gaccgtattt gaaggcttct agcagatagc cgaatttctc cgtcgcctct tcccgtgaaa
     5941 gcccaattag atcaaatacc ctgctttgaa tatctctcct gtgaatcctg actgaaccgc
     6001 ttgctatttc gtaaccgttg agaactagat cataggcttt cgatctaact ttcaaaggat
     6061 cactttctcc atatttctca agatcttcca gatccggcat agtaaaggga tgatgctcgg
     6121 cctctatcct ttgctcttct tcgttgtagg aaaacatggg aaaatccgtt acccaaagaa
     6181 actcaaatcc ctctaccgaa aactcttcct ctttaataat cttgttcctc atctgtccta
     6241 aagcctttga aagcgaaagc ccctctccta ggctgaatag aatcaggtcg ttttcattcg
     6301 cagcgaaaag agagaccaat gacgagagct cttctggaca gtgcttaagt atagatgact
     6361 tcagaccgtc accctcaact ttcagccaca tcaaacctcc aagaccgttc tcttttgcaa
     6421 atgcggtgta ttcgtcgagc ttttttcttg aaaatctatc ggccttgcct tctacgataa
     6481 agcccttgac tttctcgccc ttgtctatag aatcagagat aactttgaaa gccgtttttt
     6541 cgaagatcga agtaatatcg tgaatcttca ttccatatct agtatcgggt ttgtcgctgc
     6601 cgtagaactc aacgacctcc tgatacgtga atctcctgaa cggtgtactg atatcggcgg
     6661 aagctgcctt ctcaaacgca tatttcatca agccttcggt catcgagaag acatcttcct
     6721 catctgcaaa ggacatctcc aagtcgatct gcgtgaactc cggctgcctg tcggccctga
     6781 agtcctcgtc tctgaagcat ttggcaatct gatagtatct atcgaaaccg gaaaccatga
     6841 gcagctgttt gaatatctga ggagattgtg gaagagcata gaacttgcct gcctttagac
     6901 gagagggcac gaggaaatct cttgcccctt ctggggtgga ttttcccagg atcggtgttt
     6961 ccacatctac aaagccgtta ttgttaaggt actctctaac ggcctgaacg aatctatgtc
     7021 gggtaagaat gtttctttgc atctttggcc gtcttagatc aaggtatcta taacgaagat
     7081 gcatctcttc ggccgactct tcatcgatat ttatgtaaat cggcggcact tcggcatctg
     7141 aaagtattcc catctccgag gccacaatct ctatctcccc agtgggaagc ctaagattga
     7201 ttgcatcatc gggtcttttc ctaacggagc cagttacgga aatgcaaaac tcgttcccca
     7261 acttcaacgc catgttgtaa agctcattat cttgaggatc aaagaccacc tgtgtgaagc
     7321 catatctatc tctcacctga acaaacttga ttccgccgag gtctcttata cgatcgaccc
     7381 agccgttcaa tgtaactact ttccccgtgt cggaagccct caattcccca caggtgtgcg
     7441 ttcttttctt caactccaac acctcccaca gagataatat taccatgcca gtttctagga
     7501 tggaatctag atttgtggta gaatcattta gaatatttcc ttcggggagg tgtagaaatt
     7561 gaaaatgaaa gttttacttg cctcactgct tattctcggt ctggcattta cttcagcatt
     7621 cgctgcggtc aattacgttg atgttagttc aaatcactgg gcttacgatg ccgtgatgaa
     7681 gctttctgac ctcggtattc taaccgggat tgtgcaggcc gacggcaaaa cttatttcaa
     7741 tggaaacgat gctctgacaa ggtatcagac ggctgtcatg ttaaagaaga cgctcgatta
     7801 cgttgaactg aacttcgcca ggcagggtac cgtcccccag aacggagcgg tagatggtac
     7861 ggttatgtca agacttgaag ctttggaact ggctttgacg gattccaccg gaaggcttat
     7921 cgacaccatg gatctacagc tgagaataac cgctctcgaa aacagaatta gctctctggg
     7981 aatctctaca aacgggagtg tatctcagag cactgtcgaa tcactcagac agcagatcat
     8041 gaaattcgtt gaagatcttt ccttgaccac caaaaagctg gacatgcttg ctcttgatgt
     8101 acagaatgta cagaacagca tgtctgatct cacggttatg gaatcaaagg tcaacgatgc
     8161 cgttagaaga gttgacacac tcagtggcaa cgtaagtatg atacagagca gtatttcttc
     8221 aaacgaaagg gctatttcga ctcttgattc aactgtaagg tcattgtctt catcgttaag
     8281 tgactatgat gccaaactca gcacaataat caacagggca tctgcaaatg caatcgaaat
     8341 tgcatcgatc aaggaatcaa tgacgacact ttctggcgat gtcctgagcg tgagagatct
     8401 ccagaacaag atctcttctc ttgaagatca gattgctaat gtgaagcttc ctaccgaggc
     8461 gattgataag cttgattatc tttcggcaag agtgaacgcg atttcttcgg attacgcaaa
     8521 aatcggagat ctccagaact acgtgaccaa gaccgacctt aaggcaaact tgaatcttta
     8581 tgctgggatc gacgagcttg cgcaggttaa aaactccagc gaagtgttgt ataaggatct
     8641 ccaaaccctg agagacgact tcgcttctga aaccggaatt atcaagagcg atattggaaa
     8701 tctccaaaga agcatcaatg cgataaacga aatcgttact atccatgaaa atgaactcgc
     8761 ttctctaaag tcttcggtta gcactgtatc cactctcagg agcgacctta acgctctgaa
     8821 ttccaagttc aacgctctta ccgatcagca gtcgaagaac tttgctgcaa cacaggctag
     8881 catcggtgat ctcgaggcaa ggattaatga gaatatggat atgcttaacg tgaccgtcga
     8941 cgcgagtctt tccagaatct ctcttggaat ggatacggtt aacacacagc taggaaagaa
     9001 cgaaagtgag atcaaggcac tgaagaccag agcggattca ctagaatcga ggctaaacac
     9061 aacggtagtc aatcttgaaa gatacctcaa aattagcgac cttgagactc agcctgttat
     9121 tgtgaatctc tcggaaagag tagccgagat caacaaggcc actgttgacg ccgccacaaa
     9181 acaggatgtt gaagcacttc agaaaaagac ttcaccctgg ctgattctct ctacagtaag
     9241 ttcgcttgct gctctaggaa tatccatatg ggttcttatt gattcaggaa taatatagta
     9301 gaatagcttt cgtaatgatt gacccggggt ttaaccccgg gtttttgcta taattgaagc
     9361 tgacgagttg ttgctggagg tgcttatgaa agtcgcagag atttcttcac taggtaaaca
     9421 cataaatgaa cacgtacaga tcaggggttg gctgagaaga aaaagatcga gtgggaagat
     9481 ccagttcctc ttcttgaggg atggttccgg attcgttcag gcaatcgtag agaagtcaag
     9541 cgtgcccgag agcgtctttc aggttgcggc ctccttgaaa atggagtcaa gccttatagt
     9601 caccggtctt gtaaaggccg aggaaagagc tcccggcggt gtcgaagtac tggtagaaga
     9661 tgttcagata gttcagatcc ccgaaaagga ctttccaatt aacaagcctg accactcgat
     9721 tgattttctt atggacaaca ggcatctttg gctgaggact cagcggcaga cccatgttct
     9781 taagattcgt catgagataa tacgtgccgt cagagagttt tacaatgatc ggggctttgt
     9841 cctggtagac accccgatat tcaccgggtc aattggagaa agcgccggaa atacattcga
     9901 aatagactac ttcgattatg gaaaggcata tctagcgcag acgggacagc tttaccttga
     9961 agcggccgct atggctcttg gaaaggtcta taatcttgga ccaactttca gagcagaaaa
    10021 atcaaagaca agaagacatc tcatagagtt ctggatgaac gaagcagaag tcgcgtatta
    10081 cgggcacgac gataatatca agcttcagga ggatctcgtc acctatgtcg ttaagaaaac
    10141 tctcgaaaga gccggagagt atcttcgctc tgttggacga gatacgtcga gactagagaa
    10201 gatcgagact ccattcccta gaataaccta tgacgaggca ataaagcttc tggtaaagaa
    10261 ggggttcgat atcaaatggg gagacgacat cggaggcgat gaagaaacgg caattgcaaa
    10321 tgagttcgaa aaaccggttg ttgtcgaacg gtacccaaga atgatgaaag ccttctacat
    10381 gcagcccgat cccgagagac cggaagtagt cctatgtgat gacatgctgg ccccagaagg
    10441 ctatggagag ataatcgggg cttctgagag aattcacgac cgcgacttgc ttgtcgaaag
    10501 aatacaggag tatggtctcg atgtcgaatc atacgactgg tatctcgacc tgcgagacta
    10561 tggtacggtt ccacacagcg gcttcggtat gggaatcgaa agaattgttg catggattgc
    10621 aggtcttgaa cacatcagag aagcgatacc ctttgcaaga acgctctaca ggattcatcc
    10681 ttaggaggtt atatgcgaga agaacgcttc cttacgcccg gcgaggtaca agaggataat
    10741 tcaatcaaga gtcttaggcc taagactcta caggagtata ttggtcagaa ccagataagg
    10801 caaaggcttc gagtggcgat tgaggctgca aaggtacggc gtgaagccct tgaccacaca
    10861 ctgctggccg gccccccggg actgggaaag actactcttg cccatataat cgctaacgag
    10921 ctgggatctg agatatacgt tacttcagga cctgtcatag agaagcaagg agatctggcc
    10981 gcaattctaa ccggcctgga gcgagacgat gtgcttttca tagatgaaat tcacagactg
    11041 aacagaagcg ttgaagagat actgtattcg gctatggagg attttcagct tgacataatg
    11101 attggtaagg gaccaagcgc gagatctata cgtctcgatc tgaatccctt cactctagtt
    11161 ggtgcaacta cgagaagcgg tttcataggg gctcctcttc gaaataggtt cggtatgata
    11221 ctcgaaatgg agttctatcc ggacagggat ctaaagcaga ttattctacg ctctgccacc
    11281 cttttgggga cttcgattcg ggacgatgca gcccttctgc tcgcaagaag gtcgcgaggt
    11341 accccaagaa tcgcaaatag gctgttaagg agagtaagag acatcgtcac cgttgaaggt
    11401 gaaagcgaaa tcaccatgga gaccgttgaa tcggcaatga ctctgctttc aatagattcg
    11461 gaaggactcg attcaatgga caaaaaactc ttatcgattc tcattgaaag ctataacggc
    11521 ggacctgcag gtgtaaaggc cattgccgct tcgctaggaa ttgagccgga aacaataagc
    11581 gaagtatatg aaccattctt acttcagagt ggattcctcg taaggacaag tcggggaagg
    11641 atggttacgg aaaaggcata caggcacctc ggcctccccc ttgagggaag aaatactccg
    11701 ctctggaact acgctgatca tagagaagag gtagaggaat aggatggctg tttcttcgga
    11761 caggcccatc tataccggaa cctgtaagtc gacaaggcca tttttgctgg ataatctttt
    11821 gttttataag agcattttca gagtttgctt aggagaaagt tttccttcat ttatcgcggg
    11881 gtgagagtta tgaaggctgc agtgttctac aacagaacca gaatctccgc cgagatgctt
    11941 gactggcttt caagagagat agaattacat aagatcgata cttccttctg cgaagatcgt
    12001 gcttcctgtt ccgttccgga gcaggatgtc atccttacgt ttgggggaga cggaacggtc
    12061 ctcgaggcag tacagatggc tgttctcaac aatcttccca tcatgagctt cagagttggt
    12121 agtgtcggat tcctagctgc ctttgaactc agcatgctgc atacggccct cgaccttttg
    12181 caggagggaa aactcgatgg tgtcgatagg aatgtaatgg agataagcct tgatggaacc
    12241 gtaagatatg ctctcaacga ttgcgtcgtc gagcgttcaa caccaagcag gactgtctcc
    12301 ttgtcagtag aaatatcagg gcaaagcagc taccaagtcg taggcgatgg cataatcttt
    12361 gccacaaata cgggctccac tgcttatacg atggcggcgg gcggcgcact tgttgatccc
    12421 gaggccaatt gcttccagat aacgccaata tgcccccata atcccttcgt tggatcgata
    12481 gtcataggag cctctagaaa agtgagaatg gaagtgaaac aagacaaggg ctttcctcta
    12541 gaagcctatg tggatgggga gctcgtctct gaactgagga ccggggaaac cattgaagtt
    12601 tcactctcag atagaaaggt gactcttctc agggaaggaa gcttcgattt cgtcgctttg
    12661 ctcaagagaa ggctcgcttt tggaggtcgg cttaaggatg atatctaaag taggccttga
    12721 aagaataggt aatttgagaa tcgagatctt gagaatggcc aggtatgtcc agagcatctt
    12781 caacaagaca atggtcgcag tactagagcg aaatgaacac caggcgagag atgtgatata
    12841 ttccgattct gtgatagatt accatcaggt ggagctgcag gcaatgataa ttggtctaac
    12901 gggtatattg actccaactg gcaaagagct gaggcttttg acgctctcaa tgaatatcgt
    12961 cagtgccctg gagagaatag gtgataagtg tgtcttcatt tgcgagacaa gtctttcact
    13021 ttcgaagaga ccgcctatcg gatactacga caagatgaag tctatgtttg aatctgtctc
    13081 gattatgact aagggagcaa tagacaatct cgttgaacca tctctgtctg aagcagagag
    13141 gctctgtaag agcgatgacg ctattgatcg aatgcaagaa gaggtgaagt ctgatctgat
    13201 tcgttattgc gagcaatcgc cttcggttgt tggtagggcg tttgacctta tgatgatctt
    13261 ctgtgcactg gaagagattg ccgatctttc aacggaattg atggaagctg cagtttacat
    13321 agaaactgga agacactata gatgtgttaa cgacgatttc aagcctgtgg attttgaaac
    13381 ccagaacgaa gaggggtgat tcttcttgaa gactcttata aaatatatag gagctcaagc
    13441 gttgggttcg atatttgttg gtctcgcggg gttcataatc ttcgtaagtt tggagttgct
    13501 ttactatctc tcagatatga tcataagata caaggttgga atcgataaac tctttctact
    13561 catttattac aatcttcccg agtttattgt cctgggaatt ccagtgggaa tactccttgc
    13621 gatattctgg gtactttcaa gaatgcgctc agacaacgaa ctgattgccc ttcaaaccca
    13681 cggcatatcc ttgaagaggt tggttatacc ctttcttctc ataggtatta ttttcagtgg
    13741 ctttgcttat ctcttgaacg actatctcgt accggcagct tcgacaaagg caaatgaggc
    13801 gatggcgaga ttcgtctaca agcaaccgga agttactctc aaagaaaacg tatttatgca
    13861 ggatagccag ggaagaatga tctacgtgag gagaatcgat caggagacga aggagctcaa
    13921 gaacgtctcc atatatgaag tttcgagggg tcaagttacg cttacaacag cggaaagcgc
    13981 ggtaatctct tcgaacaaat ggattttgag caaagccaat atctatcaga cagacgaatc
    14041 gggctttctt ggcgttgaga tgcagtttgg cactgccgag ttcgaaatag atgaagatat
    14101 cgaacgttat ctggccagct tcaaatctcc aaaggaaaag acaagtaaag aactgaggga
    14161 tgatatcgaa gccttcaaga atacggggat caatacctcc tcgctcgagg tcgctctgca
    14221 agagaagtac tcgatgtcca tcgctcctct cgtcattgtt cttcttggcg ttcctgtttc
    14281 gttgatgttc aatcttcagt ctaagtcatg gtctgtcata ctcacctttc ttctggtcgt
    14341 catctatcag ggatcgggag cctggttgag tggaatggga aaggagaacc ttataaaccc
    14401 atccctggca ccatggatac cgaatattac cttttctata cttggtgtga taatatactc
    14461 cctcatcgat actaaagcca gttatagatt gtcggagctg tttacaagaa taatgaaagt
    14521 atcggcaatt gttcttcttc ttctcgttcc cggtctcctt gaaggatcta atgtgaagat
    14581 cgtcggaggt acaattgccg gaaccaagag tggcagggag atactacttg gtggtggagt
    14641 caaggtagag tatttcagcg agaccatgaa cgctacgatt gaagcctcca atgcttcgat
    14701 actaaatgtg gattccactc cggaatcgat ctccttctgg ggaaacgtga agatgctcag
    14761 tgatgagacc actattctct cggacagact gattctagac ttgacaaatg agcgcgtcga
    14821 aagtatgcag gtttactcta gaacggaact tgaggttccg gccgccagag gcgatacttc
    14881 aaaaaccggt tcgaaggtac cgttttatgt cttcggtgac tatgtgcagt cgactatgga
    14941 agcatctcct accttcaata ttggagaagg atatgtaact acatgtgaca agacccaccc
    15001 ccattaccgc ttcagagtct cctccgcggt agttacacct ggaaagggaa tgtcggtgca
    15061 gaacatgctt atgtatatcg gcaatgttcc tgtattctat cttccagctt attactttcc
    15121 tctcgacgac ccagaacgaa ggcctttcga cgttgacctt agcggcatta ccgaagccaa
    15181 gacaagaata actttacggt atttagaact ggactggtta ctcctacagg gaacctggta
    15241 cagggactgg atttccaccg aagacgcctt cactgcgaag tccgtcttat acacaccctt
    15301 gggagatttg gaactgttcg ggcagttcgg acagcagaag gaaacgagtt acggggcgta
    15361 cgttttgcta aaacccgctt tcgactggct gaggattcag ggaggagccc tttatcttag
    15421 cggtccttcg ttaaaggaac ttcaaactcc attcagtgga ataaatcttg gtgaaaccat
    15481 ctcaaagaac aaagtaactc agctcgatcc gtttgcagtc aaggaatcaa tgaatgcgac
    15541 ggagatgcag ttggcatatg ttcagcttct ctcgcctcag gactgggagc ttgaactaga
    15601 cgtaattgga gccttcgcaa gatacgatga tgagaacctt tggatggtga atcttcaaaa
    15661 ccttaccgta ccatccgatg gcagtattga tgcaggtttc ttcaagttaa gcacatcgga
    15721 gttcaatgta aacggccttt tcggtcagag atatgtgaat aataaggtgc tgtactcttt
    15781 gaggaccaag gcctcggttt tgaagacgga aagctccctt tttggtgccg acgcttcggt
    15841 agattcactg aacttcacct tggcctcaaa tgcggcaacg gtaacggaac tattcgaccc
    15901 ctcgaacatg gaggatttcg tgctggaggt gaacaaatac cttttcaccg ctggcaacct
    15961 gaagattggc ggtgatatca agagcacatt cgatgcttca actactgaac tcaatatcat
    16021 aatgccctct gacaacaaag gaattggaag aaactacttg acatatgcct cggatgacgg
    16081 gaaactgaag gtaaacggca gatatgcact ggatttcgat tcgctccatg agaagaacaa
    16141 agataaactt ttctggatag atcccttcga cattctttat gacggctttg tctcggtgga
    16201 acttaagttc tattttgaat ctagatacga caagtcattt aagattgaag gaaagaagct
    16261 tcgtgtgtac aaggatattg agctttataa aggcgaatgg ggattgcttt cggtggatgt
    16321 aatcctagaa ccagcttatg aagctggttt catatatgac tcggaagagg gttgggaact
    16381 aaccaaaaac gaaattcccg tcactctcaa gaacgaggtt acctttagac ctttcgattg
    16441 gtactatgtc ggccttcagt acaaccccgt tcttacctat gatttcataa acagggaatg
    16501 gaggctcgat catccgggaa aactgttgac cggtatcgat acggagatat tcaaggctga
    16561 ctatgccctg gaattggatt acgaagaact agtggcaagt ccaacagaga tgaactggct
    16621 tggcaaggga attcttacta ctgaggctga attcgcgtta tgggaagtga ctctaggtca
    16681 aaagaccgag actgttttta tgcctgtcac ttcacaggca tccgagacat catactcggt
    16741 cacatttgat tccgaggtct tttctcacag tacgaaaagc aaggtttact ggcagaccgg
    16801 cgacaagctt gacgactttg tcaacaagga gaggttgaca atcaaagtcg caactgagac
    16861 cagctttgaa ttcactctcg actggacttt cgacgcctca ccaagcgtgg cggacgatga
    16921 aaggatgctc aaagatcttg tttttggctc ctcgattaga gtaaaggact ttgccgaact
    16981 cggcgtcaag ttcagatatc cctatctgtt cggttcgaga aagatctatg atcgactaaa
    17041 aatagaggtc aacgatctct cgataggcga tgtttcatgg aaaaacgctt cgattgactt
    17101 cactacgggt ttttctgagt tcgactcgaa atacaagtat ccggagaagt acttcacaaa
    17161 gtctgaaacg caggcacaat tcctgttgtg gcagtcaacc aggctgtcag tttcctcact
    17221 atctgtagga gagtatttca ttgcaacttc aacggcatcg gagaccggcg ataccgccta
    17281 ttcctttgga atatcaaacg tgaagctcgg gaacaagcca ctgttgggtg ggaccaacgc
    17341 caagtttgat gaattcggac tgtcgatgga gcttagaaag agcgaggatc tgtacttcac
    17401 aatgcacatt aatgagcttt actttcctat gggttccatt tcagataccc caggcgtcct
    17461 gaacagactc tcactgggaa ttgacgggag cggaaagaga agctttgtcg aaatcgggac
    17521 atatgcggga gacatctcaa tgtgggacga gtcgatagcc aaggtctctc cgatgtattt
    17581 tgatctccac tgtatggcac tggagcttat cttgagatta agctttggca gtgatgtcac
    17641 aactattcag gacacagtcg aaagtatcgc actaaaatac tacataaagg aactgaagga
    17701 tagatacttt gtgattggcc tttacaaggg cgctccgttc ttcaggttca gattctgagg
    17761 tgaaaactgt tgactgacta tgtaaagagg aaagagcttc ttcttgaact caattcaaag
    17821 ataagtgact gtagtctttg tcctttgagc tcatcgagaa ccaacacagt tcccggtgaa
    17881 ggttcaattg aaactcccgt tgtttttgtg ggtgaaggtc ccggggccga tgaagatgct
    17941 tcaggcaggc cctttgtcgg cagggccggt gagttgctta caaagattct ggagtcagtg
    18001 aaactttcga gagaagatat tttcataaca aatatcgtaa agtgcagacc tcctaagaac
    18061 agagttccga ctgcggagga acagagagcc tgttcgccat acatgctctc tcaactagcg
    18121 ataataaggc caaagcttat cgttgcgctc ggagccacgg cactttcata ttttgtgaat
    18181 gaagagggaa ttcagataac aaaggtcagg ggacggattt tcgactggat cggcggaatc
    18241 aaggtctttg ctatgtttca ccctagttat cttctcagaa atgcatcgag ggctcccgga
    18301 tcaccaaaaa gccttacctg ggaggacatt caaaatgtta gaaagatgta tgatcaattg
    18361 ctctctggca gaaatattga catttaggag gatagaatgg ctgaaaatta cctggttact
    18421 ggaggagcgg gattcattgg ctcgcatgtc gttgatcacc tgatttcatc aggaaaaaca
    18481 ccagtcgtag tggacaatct ttcgagcggt aagatagaga atcttgatcc cagggcgctg
    18541 ttttacgagc aggacatcac cgatcaggag atgatggaaa gggtattcat gcttcacagg
    18601 cctacagttc tctttcatct tgcagctcaa ataagcgtct ctagatctgt gagagagcct
    18661 gaagaagatg cgatggtgaa tattatcggt tctttgaggc ttctgaaaat cgctgcaaaa
    18721 tacggcttga aaaaagtgat cttctcttca actggtggag cgatttacgg tgacgatgtg
    18781 aaaaggatcc ctacagatga agaggaactg ccgaaaccct tgtcaccgta tggaattgca
    18841 aagtacgcaa cggagaacta tctcagattc ttttcgagtg agcttggcat caaatacact
    18901 gttttgagat acgcaaatgt atatggcccc cgtcaggatc cgtacggaga ggccggagtt
    18961 gtagcgatct tttccgagag aatgctccat aatcaagagg tcgtaatctt cggggacggg
    19021 gaatgcgtaa gggattatgt atacgttggc gacgtcgcta gggcgaatct tctggcgata
    19081 gagaaatgtg aaaacactgt gataaacatc ggaaccggaa ttgggacatc ggtaaacgag
    19141 ttgtttgaca taatgaagcc gatcgccggt tattcaagag aagccgtgca caaggaacca
    19201 cggccgggtg acgttaagaa atcaattctc aacatagaaa gggcgagatc cttgcttgga
    19261 tgggaacctt ctactcagct tgaaaggggg attaaggaga caattgagta tttcagaagc
    19321 ggactggact cctctcagtg agagactgag gccgaaagag ttcgacgaca ttgtaggtca
    19381 ggagcacttg acgggaaaaa agggaataat ccgcggagca gtcgacagcg gcaagctctt
    19441 ttcaatgatc ctgtatgggc ctcccggcac gggaaaaaca acgataggcg aaatcattag
    19501 atcgaagctg ggaaatgact accatttcga gtttttcagc gcaagcttac agggaacggc
    19561 agatcttaag aagcattttg cccatgggga gagactgaag agagttgggc aacaactggt
    19621 gctttttgtt gacgagattc atcgacttaa caagagccag caggatgtct ttcttcccgt
    19681 aactgaaaag ggaattatta tcctcattgg agcgactaca gaaaatccta gcttcgaagt
    19741 caatcccgcc cttctatctc gctgtaggct ggtaattctt aagggtttga aggactcaga
    19801 catttcggcg cttctaaata aggcattgga aaaggatctt atcctcaagg agtccggagt
    19861 tactgttgat ggggaggtaa ttgaagtaat cgcgcaaagt agtggaggag atgcaagaat
    19921 tgccctcaat cttctagata cccttgtcga gagtgccgct gctctagaca aacccgttct
    19981 cgaccttgaa gtcatgaatg aattatctgt attgccctcg gggcgctaca cgaagaaagg
    20041 cgaagagcat tatgacatcg tctctgcatt catcaagagc cttaggggaa gcgatcccga
    20101 tgcggctctg tactacatgt taaggatgct agagggcggt gaggatccta agttcatcgc
    20161 cagaagaatg gtgatcttct cctcagaaga tattggcctc tccgatccca tggccctggt
    20221 gatagcaaac gcggcctttc aggcagtgaa taatgtcggc ttgcctgaat gcattctgaa
    20281 cctatctgaa gcggcaatct atctttcagt cgcaaccaaa agcaacagaa cctattcggc
    20341 catggtgaag gcacaagaaa aggtaaagaa taccccgaat ctagaaatcc ctctaaaact
    20401 aagaaatcca gttactaaga tgatgaaaga gttgggctat ggtaaaggct acaattatcc
    20461 gcacaactcc ggagggtttt cccgtgagta ttatctgccg gaaccgataa aggaggaagt
    20521 attctatgtt ccaggaaaca gtgggaaaga gaaattcctt tttgaaaggc tcaggtcgtt
    20581 atggaagggc atgaagaact atccggagga agaatgaaga gtttcattct tagagaaggt
    20641 agagtcattc agctcgaaat atccgatata acggaacagg aggtagacgt aatcgtcaat
    20701 gccgcgaata gttatttgag gcatggaggg ggtgttgctg gagcgttagt cagggctgga
    20761 ggaaaagaga ttcagctcga aagcgatgcc tacgttagga agaacggccc tctgaagacc
    20821 tccgaagtcg cggtaacgag cgctggaaat ctgctcgcga agaagataat ccacgttttt
    20881 ggtcctcagt acggagaact agatcttgaa tccaagcttt actgttcatt tgcaaacgtc
    20941 ctaaaaaaag ccgccgaaat gactgctgtt acgcttgcca ctccggcgat ctcaactggt
    21001 atttttggtg ttccggtgtc tatttgcgca gagcaattcc taaagtcagt gatggatcac
    21061 ttttcgcaaa acaagtcttc ttctctgagg cttataaaaa tgtgccttct cgacagaaag
    21121 gcttacgacg agtttctgtc aattgccctc gattttttca gttcaataga atgactctga
    21181 agactccctc ttccagcagg tattcggcag tcgactcatc tttggtcaac cagaccgtga
    21241 atttgtcgac ctcttgatcc ttgtattccc tggcaatcgg gtcgttctgg agaacatagt
    21301 tataatcctc ttcacttaca cgcacgatct tcggttctcc catgaaagta gactctttac
    21361 ttcgataatc ttcaaaggtt gccgtctcgt acgctctctc gccaaggaac cgaagtatag
    21421 tcgtgaagtc cttcgcggga acgtaacctg gcaggttagt caataatgcg ttttcgcttg
    21481 tgaagaacca aaatgtaggt gtgcccctga tctgaaaata agcatagagc tcaccgtatg
    21541 acatctcttt ccattcctct ccgacaacat aagaggcctt ccccggattg cttttgtata
    21601 tctgggagaa aacgaaacct gctttcaaaa gatcctgtac ggtttcatct gcgagagttt
    21661 ccttcacaaa cttcacacag tagacgcagc tggtatccga gaacattacg atagcttcct
    21721 tatcttctat cttggctagc ttaagcgctg tgtcgaaatc atctagcatt ccaacatacg
    21781 agaatattat tcctggtaga agtaaaacta gtagaaaaac aactttcttc ataaatcaac
    21841 ctcccgttgc aaactgtagt aagttaaggt ttcctgtcat tgtcaacaat ccggctgcta
    21901 ggagaacaac gaagacagat actctgatca cttttctcca tttgctttct cccccaaaag
    21961 agagctttga agttagcttt gccaccaatc cgcttagcgt caggaagggt atactcaaac
    22021 ccaacgaata gacaagaagc ataagcccgc cctcggcaac tgccgattga ttggctgcaa
    22081 gaaccagaat cgaaccaagg atcggaccgg cgcaaggcac ccaaacgaga ccaagaactg
    22141 ctccaagtat gaagccgctt acagcagaac cgccaaattt ccagacgttc aagtttactc
    22201 ccttcatgcc tatctcaaag agatacagga aagcaaacag gacgaagaga cctccagata
    22261 ttatgttcat cagcacttca ttctgcctga tgaaggttcc gagtccggtc gctcctatac
    22321 caagtatgaa gaaaaagatc gacatcccaa gagtgaagaa cactcccctg ataaaccgag
    22381 cttttgcatc ggccgcccca cccataagta caccgaagaa aactggaatc agaggaagta
    22441 tgcaaggcga gaagaaagaa agtaaacctc ccagaaaggc actcccgtaa gtgacgcttg
    22501 ttgtaacgat tggacttatc attggtgaca cacctagagt tccggttgct agtgcggtca
    22561 attcattcat attacccctc ccgggtatag tataaactat ttcccttgcg cctgcaaata
    22621 acgtctttgt gaactcctgc ttgcttgaag agaattttcc aattttacgt gagagaatgt
    22681 gacaacacta gcccgatttc ttgtctagtt cagttgcaaa ctcgagatat aagctgaaag
    22741 tatagtacaa agatagccag tccaatttag tacccttgct atatctcatg tagctagaca
    22801 aaagagttaa atcagtttca agccactaaa aagaagcatt tcaaagcaca ctctggggat
    22861 gagagtactt cacgtagaag aagctttgac agactctttc gtgacttaaa acctctcttt
    22921 aatgacattt tcagctgaaa gccattatgg gtagctgaaa aggcttttag atgaagatac
    22981 caagtgctcc acatcgtacc tcatctttcg taagtccttg gttaggaaag caatatacaa
    23041 agtcataccc gcttacttaa aatcggaaat gcagctgagt cttgctcctc tactttggca
    23101 agctcttgaa gaatctttct caacaggtga ttatgagctc ttcatttatg atattctttt
    23161 cttgaaagac tcatattaaa gaaacattcc gagtgccctt ttgttttcaa tagtgaggac
    23221 gaaaggtttt tcggttcttt caattgttga aagcagttat tgtagcggtt gattgttctc
    23281 actctcatga aagacttttc tctttggggg gagacagatt ggaaggatat gtaaccgttc
    23341 ccggtggacg aatctggtat gaagtgcaag gtagattcgg taagatgaaa cctattatta
    23401 cgattcatgg gggtcctggt gttccacaca actaccttga gacgataagt gcgctttcag
    23461 atgagaggcc cgttgtcttc tacgaccaac tgggttgcgg tagatcagag cgtccatcag
    23521 ataggtcgct ctggagagtt gatcgcttcg tggaagaaat cgagtgtctg agaaaagagt
    23581 tagggttttc gacaattaat cttctcggcc agtcctgggg aactattctc gcctgcgaat
    23641 attccgttaa ataccccagt ttcgccgaaa gtcttgttct gtcgggaccg gcaatgagta
    23701 tcaagagatt tgaaagcgat actagaaaac tggttgagac cttgtctgat cattcacaat
    23761 ccgctatcaa aagagccgaa tcaatcggta acttcaatgg agagagttat cagtcggcaa
    23821 tttcagagtt ctatgggaaa tatgtatgtc gaatggagcc atggcctcaa tgtatgaacg
    23881 aagcagtcgc cggcatgggg gaagaggtct acaactatat gtacggacca agcgaattca
    23941 cagtaacagg cgttttgaaa gacttcgact gtacacctca tctcgaaaac attaccgtac
    24001 ccgtcctatt gacttgcggt gaattcgatg aagcgacacc ggaaacgacc aggtactaca
    24061 gcagtaaatt tccaaatgca actatgaaga tcttcgaagg ggcatctcac gaacaccatc
    24121 ttgagaaagc caaggaatac atcgaggccg ttcgtaggtt tctcaaagat gtcgaataga
    24181 gatcgtggat aaacagttgt gttagtaaat atcagaatta ggggtcgaag ttaccggtat
    24241 tttacttgtc ttgaaaaaag ggtctgtaaa ctatcagttc cgaacctgcg ggaatgcttt
    24301 taactcctac tagatcggga ttatatttca tgatttcaga atcctcgatg tcatacatct
    24361 ctgaaattga tctcaatgtt tcgttccatc caaccgtgtg aatgaaccct gttcctaccg
    24421 accagttgtt gtcaacctgg ggactaatgg caaatctctc ctgaacgaaa tccgtaatta
    24481 tctcaaagat ctgaagcttc acatccctta caaccggctt attctcgaac atgctgtaac
    24541 cgccgcctcc cgcacgaaaa ctgctcatag agacttcata agtctctgcc atatcaagcg
    24601 gtttaccatt atgccagaca ctgaggactc tttcgccgac cggcctgtct atggcgatta
    24661 tgtattcaat tccctcccac atatcgtaat tcgaataatc atcaacagac caatggtcca
    24721 aagtaagcaa tccattatcg gtaacgaagc gttctgcatt gaattcaagg gcttctttga
    24781 tgtctccgcc ggttagctca agaactctga gcgtgcttgg aacaggataa acctcgtaga
    24841 tctcgccaat ggttatcggt ccggttttcc aaccggttgt cgcatgatcg aaaatcgagg
    24901 tacatgaaat cttggctccc gttacttcca tctggactct gttaatgaac tcggcaagag
    24961 ggttgtctcg aagtctcgct tcaagagcat cttcaacata gaaatcaccg actgcaaaac
    25021 caactcttgt atccagaagc ttctggattt gatcattata acctctaaag atctcgacaa
    25081 cggattcgtc aggaaggaaa ccagacatcg aaatctcatc aaccgatctg tcataaatct
    25141 tccagccttc acccgtcttt tctagataga tatcgatctt actcacggat tcgtctatat
    25201 atccaggccc agagatcaca actccatcaa cgattgccga tttagttgta agttgtgggc
    25261 ctttcaaaaa tacatctatt tccctaagag ccttcataat ctcatgggct tcttcattac
    25321 tcccacgata tgccagaacc aaaacgtcta cctcttctct aagcttgccg agataatcac
    25381 cagccgcatc gacagaatca acgaaggata gaccggcaaa aaactcggcc tcttgccagg
    25441 gcggaataac ttccgtcaca agaccaacga tgccaactct tagcttctcg ccatcgtctt
    25501 ctatctcgac gacttcaaag ggcgagaaaa ccggttctcc tgaagattta aagacgatat
    25561 ttgccgagag aagaggaaac tcagaagcag aaatagcttc ttcgaggaaa cccggtccat
    25621 aaatgaactc ttcactcccg atgagtgatg cgttgagacc gatagcgttc atcgtctcaa
    25681 cgaatggatt caaattgcag taatctattt tagccgaata gtaagagaga ggcgttcccc
    25741 gaatcaaatt tccagtatct ataaacaacg tgtttggatt gaaaagtctc atctgattga
    25801 caaaagcagc cagtcttgcc agaaaaacgt cttcactctt cccagaggaa taatcgaagg
    25861 gagcagtatt agcgggagca aaggaggtgt ggagaatcgt gaggtgaaaa tgagaagcca
    25921 tagccattgt cagaacggaa acggtaagta tcaaagcaag aagctttctc atctaaatca
    25981 ccctctcagg tccgagaaca agaatctcga cagaaaggaa agcgggatga caggtcatcc
    26041 cgcgcaagat tatctttcgc caagttgaga atcaatcatg aagatcccgt tggcttgatt
    26101 tccaaccatc ttcagtcttt tcacaacatc tctgaaattc ctttcctctt cgacttgctc
    26161 ggtaacatac cactggatga agtcgttcgt tgcgtaatcc ccctcttctt cagagatttt
    26221 cacaaggttg tggatcctgg aagtaatgta cttctcatgc tccaacccct tttcaaaaac
    26281 gtccagcaaa gactcgtatg ttccgtttgg tttttcaata cccgggatct ccgcagttgc
    26341 atcgacgtct ttaagatact gtataaactt catcgcgtgc tcttcttcct cttttgcctg
    26401 cttccacatg aagttctcgg ccccaaagta tccattcttg tgagaccagg cagccatgct
    26461 catatagata aatgcagatt cgaattccgc ctttatctgg ttattgattt ctttttccat
    26521 tctagctgta atcatagtta ccctccttaa gctgtatcaa tatcaacact tctttcatca
    26581 tattttacca ctatgcagcg aactctaaaa ttaacttaat aagaaggatg gctcaaatgt
    26641 tgccaaaagc tgttctaatg agtttataca cccagtcgtt ttctagtttg gtcgtctctt
    26701 taaatttgga ttggcaagct cataaatgta taatttctac gtcaggataa cctattccac
    26761 tacaaagggg gtaatgacag tgaggaagat cttcctgttt ttctcaatga ttctgattgc
    26821 tgcagctctt ctggcagcac cactaaaagt agccttcgtt tatgttgcac ctgtgggcga
    26881 tggtggctgg agcacaatgc ataacgaggg tagactacac ctggaaagag tcttcggcga
    26941 tcaaatcgtt actgatttca ttgagagcgt acctgaagga gctgaggcag aagttgtatt
    27001 tagaggttat gcccaaagag gatacgacct catcttcggg accagcttcg gctacatgga
    27061 cagtatggta aaggtatcac aggccttccc ggattcaacg tttatgcatt gttcaggtta
    27121 catgaccaca gataatctcg ggacctattt tggaagaatg tatgagccga gattccttag
    27181 cggtctgatc gcaggagcaa tgacgaaaac caacattatt ggttacgtcg ccgcctatcc
    27241 aatacctgag gtaatacgtg gaattaacgc ctttgcaatg ggtgtccagt acgttaaccc
    27301 cgaggcaaag gtccatgtag tctggtccaa cacctggttt gatccggcaa gagagaaaga
    27361 ggcagcggaa tctcttctgg atattggtgc agacttgata acacagcacc aggattcacc
    27421 ggctgcccag caggcagcag aagatagagg cgtcttttca ataggataca acagtgacat
    27481 gagtgccttc gcgccaaatg ctttccttgc agcccccgtt tgggactggg gtccatttta
    27541 tgaatatgta gtcagaaatg tcatggacgg aacctggaca aatgaacagt actggggagg
    27601 gatcaaagaa ggtgttgtcg acatcttcgt atccgatctt gtcccagatg gtttgacaaa
    27661 actcgtgatg accatgaggg aagcaatagt tcagggtctg ttccacccgt ttgacggtcc
    27721 agtctacgat cagtcaggag ctctaaggta tgctgaaggc gaaaaaccaa ctgacgcaga
    27781 gcttcttagc atggactggt tcgtaaacaa tgtagtcgga aagattcagt gatagttttg
    27841 gggggccgcc aggcccctcg atttctttct ttggagtgat attagttggc cgatcttccg
    27901 atcctagaaa tgagaaacat caccaaagcc tttcccggtg taatagcaaa tgaaaa
//
LOCUS       DAOQYI010000025        27122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_2077_length_27122_cov_72.171168, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000025 DAOQYI010000000
VERSION     DAOQYI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 27122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27122
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2077_length_27122_cov_72.171168"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKL93_07415"
     CDS             complement(<1..860)
                     /locus_tag="PKL93_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNQ71122.1"
                     /translation="MARHDIVKAYIDEASAEAVLFTRNSNIQWFFEGEVDPRVDISTE
                     LAAFWLLVTSSMVIALCPRYDVDRIRDEVLPKDVEIMGFDGFSNSVESVGSICSKFSK
                     VAVDSDLFFSSSNYVPIGKSFYEKTQLLSEREILRLRVTGKLTENIICEFAPELQPGM
                     TELEVEKVLRAIFIESGLEVPTLCIASDERISKSPYPVSTAKKISRYLMLRATIRKQG
                     LSVSLSRYFHFGAVPQELEQGHERASGIAAKGAVAIMRAKTMSEVFEEIKNAYASLNA
                     ADEVAYYSPGG"
     gene            1109..1462
                     /locus_tag="PKL93_07420"
     CDS             1109..1462
                     /locus_tag="PKL93_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HNQ71123.1"
                     /translation="MAIGEYVKSADWKSEKHVPVIEIPDSVKPGEAFNVQITVGKEIS
                     HPNTIEHHIKWFDLYVMYDDGQFLTHLGHIEFTPVTTQPTAVFNVKLEKSGSLIATSY
                     CNIHGLWESSKRVEF"
     gene            1481..1639
                     /locus_tag="PKL93_07425"
     CDS             1481..1639
                     /locus_tag="PKL93_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNQ71124.1"
                     /translation="MKYRCTICGYIYDPEVGDPDNGVDPGTTFEDVPEDWVCPLCGAS
                     KDDFEPID"
     gene            1693..>2351
                     /locus_tag="PKL93_07430"
     CDS             1693..>2351
                     /locus_tag="PKL93_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ71125.1"
                     /translation="MKDYVIALSGEAGQGLNTIGDMLALSLFRNGYCIFTDKSYHSRI
                     RGGEYVYRIRISDSPLYSMRQEFDLIVSLSKSTTISQLQYFHDGTKLIYDSDSDHVSS
                     KDFDFEVKAMGFPFKSIAKEAGEPRAQNVVALGGILGLFNVKREIPSKLIEETFSGKE
                     EIIKANLEALKRGYDLSLPIEIPRLDVQEHNYYLMNGTEGTGLGAIAAGCKFLAAYPM
                     TP"
     assembly_gap    2352..2451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2452..3237
                     /locus_tag="PKL93_07435"
     CDS             <2452..3237
                     /locus_tag="PKL93_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNQ71126.1"
                     /translation="PAPATGLPTRTAQEDLLFVVNAGHGEFPKYVVSPRTPKETFELT
                     EKAFYIADKYQIPSIILLTESLVDSSATIEAPIHKEEFLQRFITEGNEEYRRYEITEN
                     GISPRAIPGGKATVRVDSDEHDEAGYITEDLEIRSKMVDKRMKKMEGLLGELSAPAKF
                     NLDAPLVLVGWGDSWGAIDEFAMSRKDVGYVHYSEVFPIDPSIKEELEGKKLCSIEGN
                     FTGQFATLLRSVTGLQIDHVGRYDGRPISANWIERSLKKEGMI"
     gene            3234..4085
                     /locus_tag="PKL93_07440"
     CDS             3234..4085
                     /locus_tag="PKL93_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ71127.1"
                     /translation="MSYLREVEPAWCPGCGNFPLRMNLASTLEEMGVEKSHFIMVTGI
                     GQAAKMPHYLDINFFNGLHGRSLPVAFAIKAVNPELTVIAESGDGDMYGEGGNHFIHG
                     IRRNFDVTVLIHDNQIYGLTKGQGSPTTRKGQKTSSQLQGVINEPLNPLALAITMDAA
                     FVGRTFIGDKEHFRKVVSAAIKHKGFSVVDIMQPCVTFNKVNTFKYYKDRLYDVHEKD
                     ASYDPTDKVKAFETALEWDDKIPMGILYQNEKPTYHELHPVLKKGVILSKERSSIDVS
                     TELEAFR"
     gene            complement(4078..4755)
                     /locus_tag="PKL93_07445"
     CDS             complement(4078..4755)
                     /locus_tag="PKL93_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNQ71128.1"
                     /translation="MLNISVIVNTVAVIIGSIVGLLGGRLLKDRYRKVFFNVIGILTM
                     GLGIEMFLDTNSVLVVLGSLIIGGALGEALNIEEKIGRLAGNKHDDENFVKGFITASV
                     LFVAGPMTVIGSLKAGLSGENELIFIKSLMDGISSMMLSAALGKGVLLSAAAVYLVQG
                     GLVSLAGVLSFLQDPLYLGDFSGVGGLMLLALGIRLLEIKEIRVGNFLPALILSPVLT
                     WVSLLFT"
     gene            complement(4749..>5072)
                     /locus_tag="PKL93_07450"
     CDS             complement(4749..>5072)
                     /locus_tag="PKL93_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71129.1"
                     /translation="DEPTSGLDDRAVQRVRNLLLSEYRLGKTVVVSTHWPAEFLDMAT
                     HVMNLREGSLEYFVTVEDYVDSNLHNRQLEEKEKYLIDYYKRFKAFPENDEELIEFVR
                     REKEC"
     assembly_gap    5073..5172
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5173..5348)
                     /locus_tag="PKL93_07455"
     CDS             complement(<5173..5348)
                     /locus_tag="PKL93_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ71130.1"
                     /translation="MLTVKEVSFAYKGHGNGSQVSALKRVTFDIADGSFVILTGRTGS
                     GKSTLLQLLCGLIEP"
     gene            complement(5342..6055)
                     /locus_tag="PKL93_07460"
     CDS             complement(5342..6055)
                     /locus_tag="PKL93_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71131.1"
                     /translation="MSPESSDAILLLENLTVKVGDEEILSRFSLDVGPGERIALLGKN
                     GSGKTTLLRVVAGLKKLESGRRMLNGRIGMVFQNPDTVLFGTTVRENLFFPLRLLSIS
                     MEESRRRIELFVTEFGFSNIVDRDILTLSGGQRQLVSLAAALISEPSLLLLDEPLSML
                     DRHDRDMILRTLEEVIPQRTSMLFATNRFSEVLNCERFIILGRKGKLFDGGYRELRNS
                     FTVFEESGMSIPFEIAVSC"
     gene            complement(6036..6644)
                     /locus_tag="PKL93_07465"
     CDS             complement(6036..6644)
                     /locus_tag="PKL93_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ71132.1"
                     /translation="MKEDFQHYYTDQTEPPKTIRSVYLRLRNGHSYTFKSPEGVFAFG
                     KADRASLLLIENCILEGQESILDLGCGYGAVGITLKLEHPDLKLHMSDVNTRALTFSK
                     INARDHNILADVRKGDLFSPWEKTSFDSILSNPPIAAGKAVWQRMITEAPEFLNEGGS
                     LQIVAYHNKGGSRLEAIMRETFGNVITATKSGGVRVYVSRKF"
     gene            complement(6641..6979)
                     /locus_tag="PKL93_07470"
     CDS             complement(6641..6979)
                     /locus_tag="PKL93_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNQ71133.1"
                     /translation="MDCIFCKIASGEIKSSFVGENDWFVAFDDINPVAPTHVLVVPRK
                     HVKSLEELAKYDTECMRSFFELVEQIVELKGIKTSGYRLIVNQGSDSGQEVEHLHFHI
                     LGGRKLGRLG"
     gene            complement(6985..7650)
                     /locus_tag="PKL93_07475"
     CDS             complement(6985..7650)
                     /locus_tag="PKL93_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNQ71134.1"
                     /translation="MILALGVFLPEVKPVMDSMLVLESGEILGRHFCRGVIGNNEVVA
                     CGGFVGKIESSMLTQRLIDRFNPRLAVLSSCAAGIDESVQIGDVVVGTEFEEYDTIFP
                     LSEGKMVMKAPDTLLMRFLKVYFKAGKFGKILSGDAVVANASVRDEIHSSHGGIALDM
                     DSAPFAKTAYENKCSYLVIKTILDMADENSKRDFDRHFSELAGRSSELLVEIMKNHYI
                     DRE"
     gene            complement(7699..8100)
                     /locus_tag="PKL93_07480"
     CDS             complement(7699..8100)
                     /locus_tag="PKL93_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HNQ71135.1"
                     /translation="MEEKKELEFHVSEECNDPNCDHDHDLVSDVDRFTVTDEDGSEHH
                     FTVIAEFENNDNTYWVVEEFFEEDDEEADEEEDDASEDEEEEGYIVFRVEYDENENPY
                     LYSVEDEEFEEVSKAWNKLVEEMIAEDDEEE"
     gene            8201..8806
                     /locus_tag="PKL93_07485"
     CDS             8201..8806
                     /locus_tag="PKL93_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71136.1"
                     /translation="MRALLEIERKFLFKTKIPAEVIEEATRHLLIVQWYLNPPDRRRI
                     RVSVDESDIYVVQTLKSGAGLIREEIEKRVDLSLIIQMRKELRVSRAVMKSRHVYKRN
                     EIEGVVDWYFLPRIGYVLEVETSDPETVLPDPWNYWPLPRDSFEEVTENRSYTARSIS
                     VSINSIAGIIPERIESISDHQISEFRRLLQPIYGDSGGIDS"
     gene            complement(8786..10105)
                     /locus_tag="PKL93_07490"
     CDS             complement(8786..10105)
                     /locus_tag="PKL93_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNQ71137.1"
                     /translation="MEKYIATPEGVLPEKQESDYWCRRMENPDAVWMLKDEIESFNSA
                     IILRAREAGKLEFACSLEDCPSSVPFEEVRRSINDTIPETFFQRSLLTSEGDKVPAEI
                     RNDIIDNATAILNQDMAVRFGLVTQRTNLRVLPTDLISCDEYIDNDMLQLTAVSIGTP
                     FALLARSRDMSWLFVQTPFYRGWIKANDTATAKKRGAVMSFMKASPFLLATGNRVEIE
                     PDPFAINSLGLILQMGDRLPLEKPTENENLHAQGPFSCFTVSVPYRDRLGLLGFRKAL
                     VRMSEDVRIGDLDLTGRAILDQAFKMLGERYGWGDLYNRRDCSRFVRDIYASFGIFLP
                     RDAWAQERFGSSNKVVFEGDAEHRKSQLSSLRPGNPLYMPGHTLIYLGEENGSFFAIH
                     DGAGYRDSKGKKVSVHGVFVVDLSVRTMNSDKSYLEQLTTALRIDSA"
     gene            complement(10117..10947)
                     /locus_tag="PKL93_07495"
     CDS             complement(10117..10947)
                     /locus_tag="PKL93_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ71138.1"
                     /translation="MKAKTKTNIKKIISLVILAVLASLAFIPLYWMVATAFTQPSLTM
                     KFPPDIIPKNPTLQNFKDLFERKIIFRWTLNSFIVAGTVTLAQIFVCAMAGYALAKKK
                     FPGSKVIFSIYIASMMIPTQVTLVPLYILISKMGMVDTYQALILPAIAAPFGVFLMRQ
                     FMVSVPTEIIEAARIDGASEFKTFAKIIVPISKPAMAVLGIFTFMGQWNSFLWPLIVT
                     NTSEMRTLQAGLSLLQEQVPMQYSYLMASATYSAIPMIIVFLAFQKYFLKGITVGAIK
                     "
     gene            complement(10959..11870)
                     /locus_tag="PKL93_07500"
     CDS             complement(10959..11870)
                     /locus_tag="PKL93_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ71139.1"
                     /translation="MEQIPVKPIKRKTTLKDVWKGRSGYLFILPHFALFAIFFLVPVG
                     WGMYLSMFKYNVFSQTFVWFDNYKRILSDWLFLKSLKNTFIYTFGVVPLWLGKALLVT
                     VLIYPFRKSIQTFFKAAFYLPHVTSTVIVSMIWLWVFNPNFGLLNYFMSLLGLEPVVW
                     LGRSLTAMPSLIMMQVIMGGGSTIVLLSAAMASIPEYYFEAATLEGASSWVIFRKITL
                     PLLKPTILYSLVMGTIANFQTFSNIYIMTSGGPEFSTTTVAYLIYETAFKNYNLGLAS
                     AMSMVMFAILVVLGIIQFKWLGSNVEY"
     gene            complement(11949..13289)
                     /locus_tag="PKL93_07505"
     CDS             complement(11949..13289)
                     /locus_tag="PKL93_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ71140.1"
                     /translation="MKRLGVILLVLLMLAVPISALGKTTITWWINPWRIAPPGFPSDK
                     APTEEDFPKWAAEEFMRLHPDVEVKYVVVGNTEYSQKMAAAIATGTQPDIFKGPVWDT
                     RWVEAGLLEPIDDYLTEEDLADFYELALDAGYVDGKHYIWPWNLGTNGMGTSMLLYTP
                     DFEKAGVDWRKIVEEGWTMEEFVEIAKKLTWDSDGDGKIDHYAISFGAQDTHNLMNFI
                     YAHGAKLINEDESKVIFNSPEAVAGLQFLLDLVEVHGVAPSGVEAMGVYDVIGNFHSH
                     RTSIGFGGPYEIGRITRYVKSGQLAEAFYPVIAPFPHLEGHKGVSYASASGFVVFKQK
                     DEAKKEAVMEFARFLTNKENTALLESLIYLTARKSVNAMLFQNDAYLNEQAQTFARIM
                     DETGMEFFGSQSFPWSEISKFFTSAMQGAFGKTKTAQEALDGFVTEANRALSYY"
     gene            complement(13318..13890)
                     /locus_tag="PKL93_07510"
     CDS             complement(13318..13890)
                     /locus_tag="PKL93_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNQ71141.1"
                     /translation="MRLFIFDVGGVVAENTSVVPSISGFLGISKEEFVEIAEMDNLLA
                     LQTGRITVEEFVYVFSRKLGRPVPGNIWDMFFKPTPMEETVRIIERLRRKYRVVAGTN
                     TIESHYEIHKSRGDYELFDRVYASHLIGYAKPDKAFYSHILREENCLAEEAFFTDDTR
                     KNVKAAAELGIVSFHFTDSTALEKRLREYL"
     gene            14136..14819
                     /locus_tag="PKL93_07515"
     CDS             14136..14819
                     /locus_tag="PKL93_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNQ71142.1"
                     /translation="MNPLIFNLGRCTLFEGIKVGELERIFGSVKHRISFFHPGELIEE
                     QNSVCDEMLVLLQGQTKAQMQDFSGKVITIETMEAPSILASGFLFATENHLPVDIVST
                     AKTAVLYIERDGILWLCREYEVFLRNLLRDMGDRLTTLAEKVWMLSLNTLNQRLANFL
                     LRQSDDLGDEFELKTTKEELADALGVARPSLSRVFSEFVAQGLIKQDGRQIRILDRAA
                     LKRISGHTV"
     gene            15005..16084
                     /gene="ychF"
                     /locus_tag="PKL93_07520"
     CDS             15005..16084
                     /gene="ychF"
                     /locus_tag="PKL93_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNQ71143.1"
                     /translation="MEIGIFGLPLSGKSTLFTLLTGVEPHSSHKNEAHTAVAKVHDRR
                     VDELSKIFNPKKTVYATVSFTDIPGYDLAANQKEKNRILQFIQNSEAVMAVVRAFKDT
                     SVPWPTGAYTPVKQLETIETELLVRDMEVVEGRLLKLEEAKKKRKLSADEERETELLH
                     LIEKGFDENSFASQLELAEDDLKLLGSLALFTAKPIIVAINLDEDQFSANDYPERETL
                     LQRISERNFAHIELSGKIESELMELSEEDRGIFMEELGISESGIERLSRVVYEHMGLI
                     SFLTVGEDEVRAWTIKKNTPAKAAAGKIHTDLERSFIRAEVIPYDEFMTVRNMQEAKA
                     KGMVKIVGKEEIVKDGDIFHVRANA"
     gene            16065..16862
                     /locus_tag="PKL93_07525"
     CDS             16065..16862
                     /locus_tag="PKL93_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNQ71144.1"
                     /translation="MSEPTPDFSSLKLVDTHAHISFPQFDSDRERIIRQIEDNTISLL
                     VEIGTNVEDSERAFRTVKGLRNAFFTAGVHPHDSGGLDKQGLLKLESLLLSDKAVAVG
                     EIGLDFFRNLSPVEDQVRAFKEQLELAARLDLPVVVHVREAYSEAYEILKKSGHFKGV
                     IHAFSADLEYARMFVELGFFLGIGGPLTYKKNEELREVVREVSLDRLVCETDCPYLPP
                     VPFRGKRNEPYYVGYVIEEIANQKNISPISCSESLLNNALRLFSISL"
     gene            16871..17212
                     /locus_tag="PKL93_07530"
     CDS             16871..17212
                     /locus_tag="PKL93_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNQ71145.1"
                     /translation="MGRLEDFRSFRERMNNRILETGNIDTKRFFSLDGAVYEDGALDS
                     KTKELMGLVASAVLRCDDCIAYHIIQCKATGSSREEIMEALNIALIVGGSIVIPHFRR
                     AVELLDELEAE"
     gene            17212..19902
                     /gene="polA"
                     /locus_tag="PKL93_07535"
     CDS             17212..19902
                     /gene="polA"
                     /locus_tag="PKL93_07535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNQ71146.1"
                     /translation="MPGKLFLFDGTAIFYRGYYGIDVTLTNSKGEPTNALFGTARMLS
                     KFTRERMKNGDYAIFAFDRKEATKRHDAYESYKATREAMPDALVAQLKDIPELVEAFG
                     IKFLSIAGIEADDIVATAAKKYRDKVDEVLIVTGDKDLLQLVDDKVHILRFVTGLTDL
                     DVYDREKVLSRYELEPERLHALLSLAGDPSDNIPGVPGIGIKTAQKLLKEYGDIEGIY
                     ANIRKLSPGLRKKLIDGRSSLELSMELVKLLDNEDLNIELDDIKYEGFRKNDLRKIFL
                     KLEFSSMMNEFNLTDKADDSFAEIDGYRLVKSENELERLFEKLVSNPIFAIDLETSSL
                     DPHCAKIVGVSISIDEGSGYYIPIAHESDGWQADRDYVLKNLKKLLEDRSTKIIGQNL
                     KFDYSVLAVNGIHPVVPDFDTMLAAYLLSPDAKRFNLDELAMKFLGYKTIKFEDLMKK
                     NQLGDDFTKVPLEEAARYSVEDADISLRLSSVLRKKIYEQELEEIFRKVELALIPVLA
                     DLELNGVYFDVPSLKNLSEKYGKILDKILEELFDLTGEQFNPNSPSQVGKVLFERLGL
                     TPSKKTKGGSYSTSADALEEIADEHPAVQKLLDYRKYQKLKSTYLDSLPELVNKVTGR
                     IHTSYHQTGTGTGRLSSSNPNMQNLPIRDEEGKEIRKCVIPQKKGWKIVSADYSQIEL
                     RILAHLSQDKQLLDAFKNDKDVHSLTASNLYGIEESEVSEEQRRIGKMINFSIIYGIS
                     SYGLASRLRIPTNVAEEMISNYFKAFPEVRAFIRDTISEAKEKCFVRTMFGRKREIPQ
                     FKTKNRNVIQEGERIAINTPIQGTAADIMKMAMIKIYNLLKEKEMESFAILQVHDEMV
                     YEAPEKEIEKLKEILKEGMSEVVELSVPLDIEISVGDYWC"
     gene            19921..20202
                     /locus_tag="PKL93_07540"
     CDS             19921..20202
                     /locus_tag="PKL93_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNQ71147.1"
                     /translation="MNEKNKINWTALSTLYQFAVIVIANIVVSGGIGYLLYRFLGMGK
                     VWISFFLLFGAFSGIYNGIRYLLKEAERYDRDKDNDDKNSDNTGSPGNS"
     gene            20156..20488
                     /locus_tag="PKL93_07545"
     CDS             20156..20488
                     /locus_tag="PKL93_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71148.1"
                     /translation="MMIKTATILAALAIVESGILAFIPLPGSELGVFYGTAFSLLALL
                     LINYDAHIMITEKKRAPTGFLVRYAFYGICFATASTVSPGFFLGSFLGIMNLKIAAIA
                     FGRWLCES"
     gene            20478..21317
                     /gene="atpB"
                     /locus_tag="PKL93_07550"
     CDS             20478..21317
                     /gene="atpB"
                     /locus_tag="PKL93_07550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNQ71149.1"
                     /translation="MNLNLTKSDKIFLGVISGGYIAVLIYMVVTDYKFQLEGVGQRWI
                     YQLPFGEGPLSRVNPLTIIMTWVVMGLIIFMVTRVKVFKIIPGKKQSLLESLLEYVYD
                     LVKDSVTRKEYVRPIFDIAASLFLFILVSNIIGSFPGINAFPTENGGVTFTILADTWY
                     APTSDLNMNLMLALFVFVMSHVYAIRVKGLKSWVKSFFEPIWWMFPINIIGEIAKPIS
                     HALRLFGNIMGGAILVLILGYLVKYFVLPAALWGIFGLFFGAIQALIFTILAIAYISS
                     LIE"
     gene            21330..21575
                     /locus_tag="PKL93_07555"
     CDS             21330..21575
                     /locus_tag="PKL93_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNQ71150.1"
                     /translation="MDIGEGLMMMGKFIGAGLAMGIGAIGPGIGEGRVGASAMEAMAR
                     QPEMIGTLTTRMILADAIAETTGIYSLVIAFMILLVV"
     gene            21608..22093
                     /gene="atpF"
                     /locus_tag="PKL93_07560"
     CDS             21608..22093
                     /gene="atpF"
                     /locus_tag="PKL93_07560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNQ71151.1"
                     /translation="MIELNLTAILSILNFLLLFFVLKKLLFDKFFDIMKQRKEKISGE
                     IAEAEKLRTEANDLKREYEAKIQEARSSSNEIISQAERQAEEIVRQAREGAQQEIQRM
                     YKAAELQIQREREEAAGEIKGAIVTAAVAMVGKFLQKDMDEAARKQYARRILESMGDK
                     E"
     gene            22090..22641
                     /gene="atpH"
                     /locus_tag="PKL93_07565"
     CDS             22090..22641
                     /gene="atpH"
                     /locus_tag="PKL93_07565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNQ71152.1"
                     /translation="MKRILSLASKYANALFDSLPSENRETALSQLQQLSQSISNEELS
                     KFLFDPTVQGEKKTGILLSFVQRPLKQIERLAALLVSMKRLELLPDIENAFSARLLVE
                     GRMINVEVDSAVELTSEEEEAILEKVREKTGMQGLLKVTVDPSLIAGYVIRFSDEVID
                     TSLSGRLKRVSQELRSGPREVIE"
     gene            22638..24152
                     /gene="atpA"
                     /locus_tag="PKL93_07570"
     CDS             22638..24152
                     /gene="atpA"
                     /locus_tag="PKL93_07570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNQ71153.1"
                     /translation="MKISPSEITKVIQNQLEKTDIEFDYFEAGKVIQIGDGIARAYGL
                     KGVMANELVKFDNGVYGLALNLEEDNVGIVILGGYKEIKEGDLVRRTARIMEVPTGPE
                     LLGRVVNPLGIPLDGKGQIDTKSFRPVDVKATGVVMRKPVDTPLQTGIKAIDATIPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKGQNVKCIYVAIGQKTAALARIIDKLNQFGAM
                     EYTTIVFASASDTAALSYLAPYAGAAMGEHFMFEGQDSLVIYDDLSKHASAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARLNENFGGGSLTALPIIETQANDVSAYIPTNV
                     ISITDGQIYLDPNLFYAGNRPAINVGLSVSRVGGAAQIKAMKKIAGSLRIELAQYRDL
                     LSFAQFATELDKATRDQLTRGEKLTELLKQDQYVPMPVEEQVVVLYAGTKGYLDDLDT
                     SRVSTFETTLLKTLRASYSNVLASIREKKVIEEETEKLLKKAIEETLKLFAERG"
     gene            24159..24992
                     /gene="atpG"
                     /locus_tag="PKL93_07575"
     CDS             24159..24992
                     /gene="atpG"
                     /locus_tag="PKL93_07575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNQ71154.1"
                     /translation="MSKGKLRAIKKRIESTNSTMQITRAMEMVARAKINKIQKGLKFV
                     RLYERAMERALIRAYFGDTSHPKIEGEGEILLVISSDMGLAGAFNAEIMKAAEREITN
                     SDIRHIVTVGIKAESFFKKGDIPISSFSHFYDTPDLDEAAIIVDDALDIMESEHAGGL
                     RMVYSSFKNPLAQIPKAVKLLPLDEFSRPDNDFFDFEPEIDVLFKDVLNSWLVAKVLL
                     GMYETKVGELFSRQNAMKNATENAKNMIQQLTLVRNKLRQSNITQEIIEVVNGAEALN
                     G"
     gene            25013..26434
                     /gene="atpD"
                     /locus_tag="PKL93_07580"
     CDS             25013..26434
                     /gene="atpD"
                     /locus_tag="PKL93_07580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNQ71155.1"
                     /translation="MPDKNKQNIGKVIAITGPVVDISFEGAMLPDILNALKVKNKFLN
                     KEIVLEVAQLIGDNSVRAIAMDSTDGLVRGQEVIDTGSQVTVPVGEEILGRMFNLLGE
                     PIDEKGEVKFSDHWEIHRAAPSVTDQQTEVEILETGIKVIDLLAPFSKGGKIGFSGGA
                     GVGKTVLVMELIRNFAIEQKGLSVFAGVGERTREGNDLWLDMQEAGVIENTALVFGQM
                     NEPPGARFRVGLSAITMAEYFRDVQKKDVLVFIDNIFRFVQAGSEVSALLGRMPSAVG
                     YQPTLATEMGNLQERITSTRSGSITSVQAIYVPADDFTDPAPATTFSHLDATVNLSRS
                     IAEQGLYPAVDPLDSTSKNLDPVVVGQEHYQVARRVQEVLQRYKDLQDIIAILGMEEL
                     SEEDQLTVQRARKIQRFLTQPFFVAEKFSNMEGKYVPISETIRGFKEILEGKHDSLPE
                     QAFMMVGTIDEAKEKAKNLTKDE"
     gene            26436..26834
                     /locus_tag="PKL93_07585"
     CDS             26436..26834
                     /locus_tag="PKL93_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNQ71156.1"
                     /translation="MALKIRIITPYGIEWEGDADFISFRSTEGEIGYLPERAPALMEL
                     TVDVMKVRSGSNTLQFAVHGGYVLQEREGLTIVTDAAETPEEISVERAKQRLKRAEEL
                     LQVSKSRRERARNEAKLQRHMLRIKIGSRG"
BASE COUNT         7531 a         6088 c         6464 g         6839 t
ORIGIN      
        1 cctcccggac tgtaatacgc tacctcatcg gcagcattga gagaagcata agcgtttttt
       61 atctcctcaa agacctcact catagttttt gctctcatta tcgctacagc tccttttgcc
      121 gcaattccag aagccctctc gtgtccttgt tcaagctctt gtgggactgc tccgaagtgg
      181 aaatatctgc tcaaagaaac tgaaagcccc tgcttcctga tcgttgccct cagcatgaga
      241 tatctactga tcttctttgc agtggagact ggatagggcg atttggagat tctttcgtcc
      301 gatgcaatgc aaagagtcgg tacttcaagt cccgactcga taaatatcgc tctaaggact
      361 ttctcgactt ccagttcggt cattcccggc tggagttcag gagcaaattc gcagatgatg
      421 ttttccgtaa gtttccctgt gactctcagg cgcaggatct ctctttcact gagaagttgg
      481 gttttctcat agaagctctt gccaattgga acatagttcg aagaggagaa gaagagatca
      541 ctatcgacgg caactttcga gaacttactg cagatactcc ctacagattc tacggaatta
      601 gaaaatccat caaagcccat tatctccacg tctttgggaa gaacttcgtc tctgattcta
      661 tctacatcgt atcttgggca tagagcaatt accattgagc ttgtaaccag aagccagaaa
      721 gcagcgagtt cagtggagat gtcaactctt gggtctacct caccttcgaa gaaccactgt
      781 atattggaat ttctcgtgaa caatactgct tcagcagagg cttcatcgat atatgctttg
      841 actatgtcat gtctcgccaa acaaacccct ccagcgttct ctaaacctat tctatcagag
      901 gtaatgggaa atctatagaa gaagacagat ttcgaaggca tttttgagtt aaggataaac
      961 aatacttcta gaggttttgg aaagattacg acagacttca attattagcg atatggtata
     1021 attactgtga tttctgtacg tgttgttcag agcagctctg cagtgattgt tctttttgtg
     1081 ttcagcttta gataaaggag gggatttaat ggcaattggc gagtatgtga agagtgctga
     1141 ctggaaaagc gagaaacatg tgccggtaat tgagattccc gattcggtca agcctggaga
     1201 ggcatttaat gtacagataa cggttggtaa ggagatttca cacccaaata ctatcgagca
     1261 tcatatcaag tggtttgatc tgtacgtaat gtacgatgat ggccagttct tgactcatct
     1321 tggccacatc gagttcactc cagttacaac gcagcctacg gctgtattca atgtgaaact
     1381 ggagaagtca ggttcgttga tcgctacctc ttactgcaac attcacggtc tctgggagag
     1441 cagtaagaga gttgagttct gattaaggag gggcggcaag atgaagtaca gatgcactat
     1501 atgtggctat atttacgatc cggaagttgg agaccctgac aacggtgtag atcccgggac
     1561 cacctttgag gatgttccag aggattgggt ttgtccgcta tgtggagcta gtaaagacga
     1621 tttcgaacca atcgactgaa atgaggcggg agaatcactc ccgcctttct ttacctcgac
     1681 ggaggtgtta atgtgaaaga ctatgtaata gccctttccg gtgaagcagg tcaaggcctt
     1741 aatacgattg gggatatgct tgcgctaagt ctcttcagaa atgggtattg tatcttcacc
     1801 gacaagagtt atcattcaag gatcagaggc ggcgagtatg tgtaccgaat tcgaatttct
     1861 gactctcctc tatattcgat gagacaagag ttcgatctta tcgtctctct aagtaagagc
     1921 actactatct cacagcttca gtacttccac gacgggacga aactgatata cgattccgat
     1981 tcagaccacg tgagttctaa ggactttgat ttcgaagtca aagcaatggg ctttcctttc
     2041 aagtcgatag ccaaagaagc gggagagcca agagcccaga atgtcgtggc cctgggtggg
     2101 attctcggtc ttttcaatgt caagagggag ataccctcga aacttataga agagaccttt
     2161 tctggaaaag aagagataat taaagccaat ctcgaagcgc tgaagagagg ttacgatctt
     2221 tcattgccaa tcgagattcc ccggctcgat gtacaggaac ataattacta cctaatgaac
     2281 ggaactgagg gaaccggcct cggtgcaatc gccgcgggat gtaaatttct ggcagcctat
     2341 ccaatgaccc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncccgcaccg
     2461 gcgaccggcc ttcccacaag aacggcgcag gaagatcttc tttttgtagt aaatgcaggt
     2521 catggagagt ttccaaaata tgtggtctcg ccgcgcacac caaaagagac attcgagctt
     2581 acggaaaagg ctttctatat tgccgataaa tatcagattc cttcgattat tcttctcact
     2641 gaatcgctag tagattcctc ggccacaata gaggctccca ttcacaaaga ggagttcctt
     2701 cagagattta tcacagaagg aaatgaggaa tatcgcagat acgaaattac ggagaacggc
     2761 atttccccaa gagcgatacc cggaggaaaa gctactgtaa gagtcgattc cgacgagcat
     2821 gatgaggcag gatacattac tgaagatcta gagatcagaa gtaaaatggt cgataagagg
     2881 atgaaaaaga tggaggggtt gctgggcgag ttgtccgcac ccgcgaagtt caacctggat
     2941 gcgcctcttg ttctcgttgg ttggggagac agctggggcg caatagacga gttcgccatg
     3001 tccagaaaag atgtcggcta cgtacattac tccgaggtct ttcctatcga cccctcaata
     3061 aaggaagaac tggaggggaa aaagctctgc tcgatcgagg gaaattttac aggccagttt
     3121 gcgactctac tgagatcggt gaccggtctg cagatcgacc atgtaggtcg atacgacggt
     3181 cgtcctataa gcgcaaactg gatagagaga tctttgaaga aggaggggat gatatgagtt
     3241 atctaagaga agttgaacct gcatggtgtc ctggatgcgg gaactttccc ctcagaatga
     3301 atctcgccag taccctggag gaaatgggag tcgaaaaatc tcacttcatt atggtaaccg
     3361 gcatcggaca ggcggctaag atgcctcact atctcgatat taacttcttc aacggtctcc
     3421 atggtagaag tcttccggtc gctttcgcga taaaggctgt caatcccgaa ctcactgtta
     3481 ttgcggaatc cggtgatggt gacatgtacg gagagggcgg caaccatttt atccacggca
     3541 ttagaaggaa ttttgatgtc accgttttga tccacgataa tcagatttat gggctcacta
     3601 aaggccaggg atctcctaca acgagaaagg gccagaagac gagttctcag ctgcagggcg
     3661 tgattaatga accgctgaat ccccttgcgc tggctattac tatggacgct gcctttgtag
     3721 gaagaacctt catcggagac aaggagcact ttcgaaaggt tgtttcggct gcgataaaac
     3781 acaaaggctt ctcagtcgtc gatataatgc agccatgcgt tacattcaac aaggtgaaca
     3841 ctttcaagta ttacaaagac aggctctacg atgtccatga aaaagatgcg agctatgatc
     3901 ctacggacaa agtcaaggct tttgaaacgg cgcttgaatg ggatgacaag atccccatgg
     3961 ggatcctgta ccagaacgag aagccgacat atcatgagct tcatcccgtt ttgaagaagg
     4021 gagttatcct ttcaaaagag agatcgtcaa tcgatgtttc tactgagcta gaggccttca
     4081 ggtaaagaga agagagaccc aggtaagaac aggtgagagg atcagggccg ggagaaagtt
     4141 gccgaccctg atttctttta tctcaagtag tctgattccc agtgcaagaa gcatgagacc
     4201 gccgactccg gaaaagtccc ctagatacaa cggatcctgg agaaaggaaa gcactcctgc
     4261 aagagagacg agtcctccct gcacgagata cacggctgct gctgaaagaa gtacgccctt
     4321 acctagagct gcagatagca tcatagaaga gattccgtcc atcagcgact tgatgaatat
     4381 aagttcattt tcaccggaaa ggccggcctt tagtgatcca attacggtca ttggtcccgc
     4441 tacgaaaagg acggatgccg tgatgaagcc ctttacgaaa ttctcgtcat cgtgcttgtt
     4501 cccggcaagc ctgccgatct tttcctctat gtttagagct tctcccaatg ccccgccgat
     4561 aatcagactt ccaagaacga cgagaacact gtttgtatct agaaacatct ctattcctag
     4621 acccatggta agaattccga tgacgttaaa gaagaccttt ctataacggt ccttcagaag
     4681 tcttccaccg agcaatccaa ctatagaacc tatgatcacg gcaaccgtgt ttacaatgac
     4741 agatatattc agcattcctt ttctctcctg acgaattcga tcagctcttc atcattctcg
     4801 ggaaaagcct tgaagcgttt gtagtagtct atcagatact tctccttctc ttcaagctgc
     4861 ctattatgaa gattactgtc gacatagtct tctactgtaa cgaaatattc aaggctgcct
     4921 tctctcagat tcattacatg agtagccata tccaggaact ctgcaggcca gtgtgtagat
     4981 acaactactg tctttcctaa ccggtactcg gacaaaagga ggtttcttac tctttgaacg
     5041 gctctatcat ccagccccga tgtcggttca tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnggttcaat caatccgcaa agaagctgaa gaagagtaga tttaccggac
     5221 ccggtccttc ccgtcaatat gacaaaggaa ccatccgcaa tatcaaaagt gaccctcttc
     5281 aaggcggaaa cttgactccc gtttccatga cctttgtagg caaaggaaac ttctttaaca
     5341 gtcagcatga gacagcaatc tcgaagggga tcgacatccc cgactcctcg aacacggtaa
     5401 aggaattcct gagctctcta taacccccat cgaagagttt tccctttctt cccagaatta
     5461 tgaatctttc acagttcagg acttcggaaa acctgttagt tgcaaagagc atcgacgtcc
     5521 tctgcggaat aacctcttca agagtccgta aaatcatgtc tctatcatgc cggtccagca
     5581 tcgataacgg ttcatccagc agaagaagag agggctcaga tatgagagcg gccgcaagcg
     5641 aaaccagttg acgctgtcct ccggagagcg tcagtatgtc cctatctacg atatttgaga
     5701 agccgaattc agtgacgaag agctctatcc ttctacgcga ctcttccata gaaattgaaa
     5761 gcaagcgaag aggaaagaag agattctccc gaacagtagt accgaagagc accgtgtcgg
     5821 gattctgaaa gaccattcca attcttccgt ttaacattct tcttcccgat tcaagttttt
     5881 tcaaaccggc tacaaccctt agcaaagtag tctttccgga gccgttcttg ccgagcagag
     5941 ctatgcgttc gcccggccca acatcgaggc tgaacctcga gagtatctct tcgtcgccca
     6001 ctttgacagt taagttttcc agaaggagaa tggcatcaga actttctgga gacatagacc
     6061 ctaacaccgc cggattttgt cgcagttatg acgtttccaa atgtttctct cattatcgct
     6121 tcaagtcttg aaccaccctt gttatggtac gccactatct gaagtgagcc tccctcattt
     6181 agaaactcag gagcctcagt tatcatcctt tgccacactg ccttaccggc agctatcggg
     6241 ggatttgaaa gaatggaatc aaagcttgtc ttttcccagg gagagaagag gtcgcctttg
     6301 cgaacgtccg ctagaatatt gtgatctctc gcattgattt ttgaaaaggt caaggctctg
     6361 gtattgacat cgctcatgtg gagtttcaga tctggatgtt ccagcttgag tgtgatgcct
     6421 accgcgccgt aaccgcaacc cagatcgaga atgctctcct gaccttcaag aatgcagttc
     6481 tctattagca agagtgaggc tctatccgcc ttcccaaagg caaacactcc ctcgggactt
     6541 ttgaatgtgt aagaatggcc gtttctaagg cgaagataaa cggaccggat ggttttcggc
     6601 ggttcggtct gatctgtgta gtagtgttga aagtcttcct tcatccaagt cttcccagct
     6661 ttcttcctcc gagtatgtga aagtgaagat gctctacttc ctgtccggaa tcactgccct
     6721 ggttgacgat tagcctgtaa ccgctcgtct ttatgccctt cagttcgact atctgttcta
     6781 cgagctcaaa aaagcttctc atgcattctg tgtcatactt tgccagctct tcaagactct
     6841 tcacatgttt gcgaggcact accagaacat gagtgggagc aacgggattt atgtcgtcaa
     6901 aggcaacaaa ccagtcattt tcccccacga aactactctt aatttctcca ctggcaatct
     6961 tgcaaaatat gcagtccacc tttctcactc cctgtctata tagtgattct tcattatttc
     7021 gacgagtagt tcagatgacc ttcccgcaag ttcagagaaa tgcctatcga aatccctctt
     7081 tgaattctca tctgccatat ctagaattgt ttttattaca agataactac atttattctc
     7141 gtaagcggtc tttgcgaacg gcgcagaatc catatcgagg gctatacctc catgggaaga
     7201 gtgtatttcg tctctaaccg aggcatttgc gaccaccgca tcacccgaaa ggatcttacc
     7261 gaattttcct gccttgaagt agaccttgag aaacctcatg agaagcgtgt cgggcgcttt
     7321 cataaccatc ttaccttcgc taagtgggaa gattgtgtca tattcctcga actcggtacc
     7381 tacaacaaca tcgcctatct gcactgattc atcgattccc gccgcgcatg aagaaagaac
     7441 ggccaatctc ggattgaagc gatcaatcaa tctctgagtg agcatcgaag actcgatctt
     7501 tccaacaaaa ccaccacagg caacgacttc attgttacca atgactcctc tgcaaaagtg
     7561 tcttccaagt atctctcctg actccagcac gagcatactg tccattacag gttttacttc
     7621 tggaagaaag acgccgagag caagaatcat gtcaacacct ccatagagga ttataacaaa
     7681 aaggggacct caggtcccct attcttcttc atcatcctct gctatcatct cttcgacgag
     7741 tttgttccag gctttggaaa cctcttcaaa ctcctcgtct tcgacggagt aaaggtaagg
     7801 gttttcgttc tcgtcgtact cgaccctgaa aactatataa ccttcctctt cttcatcttc
     7861 ggaagcgtca tcttcctctt catcggcttc ctcgtcgtcc tcctcgaaaa actcctcaac
     7921 tacccagtaa gtattgtcgt tgttctcgaa ttcggctatt acagtgaagt ggtgttcgct
     7981 tccgtcttcg tccgtgactg taaatctgtc tacatcacta actagatcgt ggtcgtgatc
     8041 gcagtttgga tcgttgcatt cttcagaaac gtgaaattca agttcctttt tctcttccat
     8101 cataaacctc cgtttgtcac tattcggtac atactaacac aggaccgaag aagacgcaac
     8161 ttgccgatgt gaatgtattg atatacactg tttctggagt gtgagggcat tgctcgaaat
     8221 cgaaaggaag tttcttttca aaacgaagat tccggcagag gtgatagaag aggccacaag
     8281 gcatctgctg atagttcagt ggtacctcaa ccctcctgat aggcgtcgga taagggtcag
     8341 tgtcgatgaa tcagatattt atgtggttca gaccttgaaa tcgggagcag gcctcatcag
     8401 ggaagagatc gagaaaagag tcgatctttc actgataatt caaatgagaa aggaactgag
     8461 ggtctcaagg gccgttatga agtcgaggca tgtttacaag agaaatgaaa ttgaaggtgt
     8521 cgtcgactgg tattttctac cgcgtatcgg ttacgttctc gaagttgaaa cctctgatcc
     8581 ggaaacagta ctgcccgatc cctggaacta ctggccgctt ccccgtgact cttttgaaga
     8641 agttaccgaa aatcggtcat atactgcgag atcgatctct gtatcaatca acagcatagc
     8701 gggaataatc ccggagagaa tcgaatcgat ctccgatcac cagatcagcg agttccgtag
     8761 acttcttcag ccaatttatg gagattcagg cggaatcgat tcttagcgcc gtcgtaagct
     8821 gctccagata gctcttatcg ctgttcatag ttcggaccga tagatcgacc acaaagacac
     8881 cgtgcacgga aacctttttg ccctttgaat cccggtagcc cgccccgtca tgaatcgcaa
     8941 agaaacttcc attctcctcc ccgaggtaga tcagagtatg tccaggcata tacaaaggat
     9001 tacccggcct caaagaggac aactgagact ttctatgttc agcatcgccc tcgaagacta
     9061 ctttgttgct tgatccaaac cgttcctgcg cccaggcatc tcgcggtaaa aagattccga
     9121 agcttgcata gatgtctcga acgaatctcg aacaatccct cctgttatag aggtcacccc
     9181 agccatatct ctctccgagc attttgaagg cctgatccag gattgctctt cctgttaaat
     9241 ctagatcgcc aattcggacg tcttcgctca ttctaacgag tgctttccta aagccgagca
     9301 aaccgagcct gtctctgtac ggaactgaaa cagtgaaaca ggagaatgga ccctgagcat
     9361 gaaggttttc gttctccgta ggcttctcaa gaggcagcct gtcacccatc tgcagaatga
     9421 gcccgagaga gtttatagca aagggatctg gttcgatctc caccctgttg cccgttgcga
     9481 ggaggaatgg agaagctttc atgaaggaca taacagctcc tcttttcttg gctgttgctg
     9541 tatcgtttgc cttgatccat cccctgtaaa agggcgtctg aacaaagagc catgacatat
     9601 ccctgcttcg ggcaaggagt gcaaatggag tcccgatact cacggccgtt aactggagca
     9661 tatcgttatc tatgtactca tcacacgaaa taagatcggt tgggagaacc ctgagattcg
     9721 ttctctgagt caccaaaccg aatctcacgg ccatatcctg atttaagata gcggttgcat
     9781 tatcaatgat gtcgtttcga atctcggccg gaactttgtc tccttccgag gtgagaagag
     9841 atctttggaa gaaggtttcg ggaatcgtat cgttaatcga tctcctaact tcctcgaaag
     9901 gaaccgaaga tggacagtct tccaaactgc aagcaaattc taatttcccg gcctctctag
     9961 ctcgtagaat tattgcgctg ttgaaggatt ctatctcgtc cttcaacatc caaactgcgt
    10021 ccgggttttc cattcttcta caccagtaat cagactcttg cttttctggt aacactcctt
    10081 caggtgtcgc aatatacttt tccatggccc actccttcac tttatcgctc ctaccgttat
    10141 ccctttcaag aaatactttt ggaaggcaag gaaaactatg atcatcggta tggccgagta
    10201 tgtagcgctt gccataaggt atgaatactg catcggtacc tgctcctgga gaagagaaag
    10261 accggcctga agggttcgca tctcggaggt gttcgtaact ataagtggcc acaagaagct
    10321 gttccactgt cccatgaatg tgaatattcc tagaacagcc atagccggtt tagaaatcgg
    10381 gacaatgatc ttcgcaaagg ttttgaactc gcttgcgccg tcaattcttg ccgcttcgat
    10441 aatctctgtg ggtacagata ccatgaactg tctcataagg aagactccaa aaggcgcggc
    10501 aatagcgggc agaatcagcg cctgataagt gtccaccatc cccattttcg atatcaagat
    10561 gtacaggggc acaagggtta cctgggttgg tatcatcatc gatgcgatat atatcgagaa
    10621 tatcaccttg cttccaggga atttcttctt cgcgagagcg tagcctgcca ttgcacagac
    10681 gaaaatctga gcaagagtaa ccgttccggc aacaataaaa ctatttagag tccatctaaa
    10741 gattatcttt ctctcgaaga gatccttgaa attctgaaga gttggattct tagggatgat
    10801 atctggtgga aacttcatag tcagtgaagg ttgcgtaaaa gccgttgcaa ccatccagta
    10861 aaggggaatg aaggcaagtg atgcaagcac cgccaaaata accagcgaaa ttatcttttt
    10921 tatgttagtc ttagtcttcg ctttcacagc caatcacctc aatactccac atttgagccg
    10981 agccacttga actggattat tccaagaact accagtatcg caaacataac catcgacatt
    11041 gcagaagcaa ggccgagatt atagttctta aaggcggtct cgtaaatcaa ataggcaacg
    11101 gtcgtggtgg agaattccgg tccgccactc gtcataatgt atatgttcga gaaggtctgg
    11161 aaattcgcta ttgtacccat tacgagagag tagagaatag tcggtttcaa gagaggtaag
    11221 gtaatctttc tgaagatcac ccaggaactg gccccttcaa gcgttgcagc ttcaaaataa
    11281 tactctggta tcgatgccat ggccgcagag aggagcacta ttgtcgaacc cccacccatg
    11341 ataacctgca tcattattaa ggatggcatt gccgtcaagg accttccaag ccacactact
    11401 ggttccagac cgagcaaaga cataaaatag ttgagtagtc cgaagttagg attgaaaacc
    11461 caaagccaaa tcatggaaac aatgaccgta gaagtaacgt ggggcagata aaaggctgct
    11521 ttgaaaaatg tttggatgct ctttctaaac gggtaaatga gaaccgtgac aagcaaggcc
    11581 tttcctaacc acagtggaac cactccaaaa gtgtatataa aggtgttttt cagtgatttt
    11641 agaaacagcc agtcgctgag aatacgtttg tagttgtcga accagacaaa tgtctgagaa
    11701 aagacattgt acttaaacat actcaggtac atcccccaac caaccggtac aaggaagaat
    11761 atcgcaaaga gggcaaagtg cggaagaatg aagagatatc ccgatctgcc cttccatacg
    11821 tctttcaacg tagtctttcg ttttattggt ttaactggaa tctgctccaa atcgaccacc
    11881 cccattaaat gggaacagcc ccgggccctt ggggcagagg ctgttccctt caactcattt
    11941 gattagaatc aatagtaaga gagtgcccgg ttggcttcgg ttacaaagcc gtccagggcc
    12001 tcctgcgctg tcttagtctt tccgaaagcc ccctgcattg cgctggtgaa gaacttggat
    12061 atctcagacc acgggaagct ctgtgatcca aagaactcca ttccagtctc gtccattatc
    12121 cttgcgaatg tctgagcctg ctcgttcaag taagcatcat tctggaagag catggcattt
    12181 acagactttc ttgcagtgag atatataagg ctttccagaa gagcggtgtt ttccttgttc
    12241 gtcaggaatc tagcgaactc cataacggct tccttctttg cctcatcctt ctgtttgaag
    12301 acgacaaatc cactggccga tgcataagat acgcccttat gtccttcgag atgagggaac
    12361 ggagcaatca ccgggtagaa ggcttcggca agctggccgg attttacata acgtgtgatt
    12421 cttccaattt cgtaaggacc gccaaaacct atactggttc tgtgagagtg aaagttccca
    12481 attacgtcgt aaacacccat agcctcgact ccgctaggtg caactccgtg aacttcgacc
    12541 agatcgagaa ggaactgaag tccggcaact gcctctggtg agttgaagat gaccttactt
    12601 tcatcttcgt tgataagctt tgcaccatgt gcatagatga aattcatgag gttgtgggta
    12661 tcctgtgctc caaagctgat cgcatagtga tcgatctttc catctccatc ggagtcccat
    12721 gtcagcttct tcgcaatctc cacaaactcc tccatagtcc agccttcttc aacgatcttc
    12781 cgccaatcga ccccggcctt ttcgaagtct ggagtgtaga gaagcatgct cgttcccata
    12841 ccgtttgttc cgagattcca cggccagatg taatgcttgc catcgacata acctgcatca
    12901 agagcgagtt catagaagtc tgcaaggtct tcttcggtaa ggtagtcgtc gataggttcc
    12961 agaagtcccg cttcgaccca tctggtatcc caaacggggc ccttgaagat gtcaggctgg
    13021 gtcccagtcg caatggccgc agccattttc tgagagtatt cggtgtttcc aacaacgaca
    13081 tacttgactt caacgtctgg atgaagtctc atgaactcct cagctgccca cttcgggaag
    13141 tcctcctccg ttggggcctt atcggacggg aagccaggag gtgcgattct ccaagggttg
    13201 atccaccagg tgatggtggt ctttccaagc gcagaaatcg gtaccgcgag cattagcaat
    13261 actagaagaa ttacgccaag ccttttcata gtaacacccc tttcgatgtg aagttactca
    13321 aaggtattct ctgagtcttt tttctaaagc tgttgaatca gtaaaatgaa aagaaacaat
    13381 tcctagttcg gcagcggcct taacgttttt tctcgtgtca tccgtgaaga aagcttcttc
    13441 tgcgaggcaa ttctcttcac gaaggatatg actgtaaaat gccttgtctg gttttgcata
    13501 tccgatcaaa tgtgatgcat ataccctgtc gaagagttcg tagtcgcccc gcgacttgtg
    13561 tatttcgtag tgtgactcga ttgtatttgt gccggcaacc accctatatt ttcttcgaag
    13621 tctctcgatt attctgacgg tctcctccat tggagtcggt ttgaaaaaca tgtcccagat
    13681 attccccggg accggtctac cgagctttcg agagaaaacg taaacgaatt cctctaccgt
    13741 gattcttccc gtctgcaaag caaggagatt atccatttcc gcaatctcca caaactcctc
    13801 tttcgagata ccgagaaagc cgcttatcga cggaaccaca cttgtatttt ctgcaacaac
    13861 cccacccaca tcgaatatga aaagtctcaa gattgcctcc attatgtctt tacaagagta
    13921 caagctgcgg actcaaaaac ttgagattag ctaaaatgct gacccgcaac aactcctaaa
    13981 gaagatatca aagtatacta aaaatatcat actatttaat ggatgccaaa atcaagcgct
    14041 taagacttaa actgctggaa aacagcaaac cgaacaccaa aaaacaacta atctggttat
    14101 aatcaaaatg aatcgacctt tcacgggggt gacttttgaa tccattaatt ttcaatctgg
    14161 gaagatgcac tttgtttgaa gggataaaag tcggcgagct tgaaaggatc ttcggaagtg
    14221 tgaagcacag gatcagtttc ttccatcccg gagaacttat cgaagaacag aactcagtct
    14281 gtgatgaaat gctcgtcctt cttcaaggac agacaaaggc gcaaatgcag gacttttccg
    14341 gcaaggtcat aacgattgaa acgatggagg ctccgtctat acttgcttcc ggctttctct
    14401 tcgctacaga gaatcatctt ccagtagata tagtctcaac agcgaaaacc gcggttctct
    14461 acatcgaaag agatgggatt ctctggcttt gcagggaata cgaggtcttt ttgagaaacc
    14521 tgcttaggga tatgggagac agactgacta ctctggcaga aaaggtgtgg atgctctctt
    14581 tgaacactct gaatcaaagg ttggcaaact ttcttctcag acaaagtgac gatctgggag
    14641 atgagttcga actgaagact acgaaggaag agcttgctga cgctcttgga gtggccagac
    14701 cctctttgtc aagagttttc tctgagtttg ttgcgcaggg gttaatcaag caggacggaa
    14761 gacagataag gattctggac agagccgccc tgaagaggat cagcggtcac acggtgtagc
    14821 ccctttttca attccaagct acgtttcagc agtgttctaa ttgcactacg gcgttctgca
    14881 gtgaatctat ttcatggatg aagggacttt atagaaccct caccttccct cgcttgtctt
    14941 gaatctggct ttccattgca gtcgctattc tgataatata gatgtgtcaa aaggaggcgt
    15001 tgttatggag ataggcatct ttggtctacc gttgagcggg aaaagcacgc tgttcaccct
    15061 cctaactggt gtcgaacccc attccagtca caagaacgag gctcatacgg ccgttgcgaa
    15121 agtacacgac agaagagtag atgaactttc gaaaatcttc aatccaaaaa agacagtata
    15181 tgcaacagtg tcattcaccg atattcccgg ctacgatctt gcggcaaacc agaaggagaa
    15241 gaatcgcatt ctccagttta ttcagaattc agaggccgtt atggccgttg tccgagcctt
    15301 caaagacaca tcagttcctt ggcccactgg agcctacact cccgtcaagc aattggagac
    15361 gattgagacc gagcttctcg taagagacat ggaagtagta gagggcagac tcttaaaact
    15421 tgaagaagcg aagaagaaac gaaagctatc tgccgacgaa gaaagggaga ctgagcttct
    15481 ccacctcatc gagaagggct tcgacgagaa cagtttcgca tcgcaacttg aacttgctga
    15541 ggatgatttg aaactacttg gttcgcttgc cctcttcacg gcgaagccga taatcgtagc
    15601 cataaatctg gatgaagatc agttttctgc aaatgattat ccagagcgtg agacccttct
    15661 tcaaaggata agtgaaagaa acttcgcgca cattgaactt tcaggaaaaa tagagtccga
    15721 actcatggaa ctatccgagg aggacagggg gattttcatg gaagagcttg gaatctccga
    15781 aagtggcatt gagagattgt cgagagtggt ctatgaacac atgggcctga tttccttctt
    15841 gaccgtcggc gaggatgaag ttagggcatg gacaataaag aaaaatactc cggccaaggc
    15901 tgctgcgggg aagatacaca cggatctgga gaggagtttc atcagggcag aagtgatacc
    15961 atacgatgag ttcatgacgg taaggaacat gcaggaggca aaggcaaaag gcatggtgaa
    16021 gatcgtaggc aaggaggaga tcgtgaagga cggtgatatc tttcatgtca gagccaacgc
    16081 ctgatttctc ctcactgaag cttgtcgaca ctcacgcaca catctccttt ccccagtttg
    16141 attcagatag agagagaata atcaggcaaa ttgaagacaa caccatatct cttcttgttg
    16201 agatcgggac aaatgtggag gactccgaaa gggccttcag aaccgtgaaa ggcctgcgaa
    16261 atgctttctt cacggccgga gttcatcctc atgacagcgg cgggctagat aaacagggtc
    16321 ttctcaaatt agaatctttg cttttgagtg ataaagccgt tgctgttggc gagataggac
    16381 tggatttctt caggaatctt tctcctgttg aagaccaggt aagagccttc aaagaacagc
    16441 ttgaactggc ggccaggctg gatcttcctg tcgtggtgca cgttcgggag gcctactccg
    16501 aagcttacga gatactcaaa aaatccgggc acttcaaggg agtaattcac gccttttccg
    16561 ctgatttaga atatgcgaga atgttcgtgg agctgggatt cttcctcgga atcggcggac
    16621 cattgacata taagaagaat gaagagctca gggaggtcgt aagggaagtc tctcttgaca
    16681 gactcgtttg cgaaaccgac tgtccttatc ttccgccagt tcctttcaga ggtaaacgga
    16741 acgagcccta ttacgtcggc tatgtcatcg aagaaattgc gaatcagaaa aacatttcgc
    16801 cgatcagctg ttcggaaagc ctcttgaaca acgcattgag actattctct atctcacttt
    16861 aggaggaaaa atgggaagac ttgaagattt cagaagtttt cgagaaagga tgaacaatag
    16921 aatccttgaa actggtaaca tagatacgaa gcgtttcttc tcgctggacg gcgctgttta
    16981 cgaggatggg gcgctggatt cgaaaactaa agagttgatg ggtcttgtgg cttcggcagt
    17041 tctcaggtgt gatgattgca tcgcttatca cataattcaa tgcaaggcaa cggggtccag
    17101 tagagaagag atcatggaag cgctcaacat agcactgata gttggggggt cgatagttat
    17161 tcctcacttc aggagggctg ttgagctact cgacgaactg gaggcagagt aatgcccgga
    17221 aaactgtttc tctttgatgg aactgcaatc ttctaccgtg gttactacgg gatagatgta
    17281 acactcacta attccaaggg tgaaccaaca aacgctctct ttggaacggc gaggatgttg
    17341 tcgaaattca ctcgagagag aatgaagaat ggcgactatg ctatcttcgc ttttgaccga
    17401 aaggaagcta cgaagcgtca tgatgcgtac gaatcataca aggcaactcg tgaggctatg
    17461 ccagatgccc ttgttgcaca actcaaggac attcccgaac tagtcgaggc cttcggcata
    17521 aagtttcttt cgatagccgg aatcgaagcc gacgatatcg tcgcaacggc tgcgaagaag
    17581 taccgtgata aagttgacga agtgcttata gtaaccggcg acaaggatct tcttcaactg
    17641 gtggacgata aggttcacat tttgcgtttc gttacgggtc ttaccgatct cgatgtatac
    17701 gacagagaga aggttttgtc caggtatgaa ctggaaccgg aaaggctcca tgctcttctt
    17761 tctctcgcag gtgatccgtc ggataacata cccggtgttc caggaatcgg aataaagacc
    17821 gcccagaagc ttctcaaaga atacggtgac atcgaaggaa tctatgcaaa catcaggaaa
    17881 ctcagccctg gactgagaaa gaagttgata gacggcagaa gctcgcttga actttcgatg
    17941 gagcttgtaa agctacttga caatgaggat ctgaacatag agctcgacga cataaagtat
    18001 gagggtttca gaaagaacga tttgcgaaag atcttcctaa aactcgaatt ctcatctatg
    18061 atgaatgaat tcaacctaac cgataaggcc gatgattctt ttgcagagat agatggatac
    18121 agactagtca agagcgaaaa tgaactcgag aggcttttcg agaaactggt ttccaaccca
    18181 atattcgcaa tagatctcga gacttcctct ctcgatcctc attgcgcgaa gatcgtagga
    18241 gtctcgattt ccatcgatga ggggtccggt tactacattc cgattgcaca cgagtctgac
    18301 gggtggcagg cagaccgaga ctatgtcctg aaaaatctta agaagcttct tgaagatagg
    18361 tcgactaaga taatcgggca gaatttgaaa ttcgattatt ctgtcttagc agtcaacggt
    18421 atccatccag ttgttcccga ctttgacacg atgctggctg catatctcct ttctccagat
    18481 gcaaagaggt tcaatctcga tgagctagcg atgaaattcc tcgggtacaa aacgataaaa
    18541 ttcgaggatc taatgaagaa aaatcaactg ggcgatgatt tcaccaaggt acctctggaa
    18601 gaggctgccc gctattcggt ggaggacgcg gatatttcgc tcaggctttc ttcggttctt
    18661 agaaaaaaga tatatgaaca ggaacttgaa gagatattta gaaaggtcga actggcacta
    18721 attccggtgc ttgccgacct cgaactaaat ggtgtttact tcgacgttcc ttcgctcaaa
    18781 aacctttcag agaagtacgg aaagattctc gataaaatcc tcgaggagtt gtttgacctt
    18841 acgggagagc agttcaatcc caactcccct tcacaggttg ggaaggttct ctttgaaaga
    18901 cttggcctca caccttcaaa aaaaacgaag ggtggctcct actcgacgag cgccgacgct
    18961 ttggaagaaa tcgcagatga acatcctgcg gtgcagaagc tcctcgatta taggaagtac
    19021 cagaagctga aatcgactta cctggactcg cttcccgaac tcgtcaacaa agtgacagga
    19081 agaattcaca ccagctatca ccagacggga acggggacgg gaagactgtc aagcagcaac
    19141 ccaaatatgc agaatctacc cattagggac gaagagggaa aagaaatcag gaaatgcgtc
    19201 attcctcaga agaagggctg gaagattgtg agcgcagatt actcccagat cgaacttagg
    19261 attctcgcac acctaagcca ggacaagcag cttcttgacg cttttaaaaa cgacaaagat
    19321 gtacacagtt taacggcatc aaatctttac ggaattgaag aaagcgaagt aagtgaagag
    19381 cagagaagaa tagggaagat gataaacttc tctataatat acggaatctc ttcctacgga
    19441 ctggcaagca gactaaggat tcccacaaat gttgccgaag agatgatttc gaattatttc
    19501 aaagcctttc ctgaggtaag ggccttcatt agggacacaa tttctgaggc caaagaaaag
    19561 tgctttgtga ggacgatgtt tggcagaaag cgggagatcc ctcagtttaa gactaagaat
    19621 agaaacgtaa tccaggaagg cgagagaata gctatcaata cgcccattca gggaacggct
    19681 gctgacataa tgaagatggc catgataaag atctataacc tgctgaaaga aaaagaaatg
    19741 gaaagctttg ctattctcca ggtacacgat gaaatggtct atgaagctcc agagaaggag
    19801 attgaaaagc tgaaagagat tcttaaggag ggaatgtctg aggttgtaga actgagcgtt
    19861 cccctggata ttgaaatctc tgtaggtgat tactggtgct gaaagggaga cgtcacccct
    19921 ttgaatgaga aaaacaaaat aaactggacg gcgctgtcaa cgctttatca gtttgcagtt
    19981 attgtgatcg cgaacatagt ggtttcgggg ggtataggat acctactgta taggttcctc
    20041 ggaatgggta aggtatggat ttcttttttc ctacttttcg gagccttctc gggcatatat
    20101 aatggaataa gatatctatt gaaagaggca gaacgatatg atagagacaa ggacaatgat
    20161 gataaaaaca gcgacaatac tggcagccct ggcaatagtt gaaagcggta tccttgcctt
    20221 tataccgctt cccggttctg aactgggtgt cttttacgga actgcatttt ccttgctagc
    20281 tctgctgctg ataaattacg atgcacatat catgataacc gagaaaaaga gagccccgac
    20341 cggtttcctc gtaagatacg ccttctatgg catttgtttt gcaactgcat ctacagtttc
    20401 acccgggttc ttcttaggaa gttttctcgg aatcatgaat cttaaaatcg cagcaatagc
    20461 ctttgggagg tggctctgtg aatcttaatc tcactaaatc cgacaagata ttcctcggag
    20521 ttatttctgg aggatatatc gccgttctca tatatatggt tgtcacagac tacaagttcc
    20581 agcttgaggg cgttggtcag cgatggatct atcagcttcc ctttggagaa ggtcctctca
    20641 gcagggtaaa ccccttaacg ataatcatga cctgggtagt aatgggtctg ataatattta
    20701 tggtaacaag agtgaaggtc ttcaagatta tcccgggtaa gaaacagagt cttctcgagt
    20761 ctctcctcga atacgtttac gatcttgtga aggattcagt gactagaaag gaatacgtca
    20821 ggccgatctt cgatattgcg gcctcacttt tccttttcat tctggtctcg aatattatcg
    20881 gcagtttccc tggaataaat gccttcccta cggaaaacgg aggggtcaca ttcacgatcc
    20941 tcgccgatac gtggtatgct cctacatcgg acctcaacat gaatctcatg ctcgctttat
    21001 tcgtgttcgt catgagtcat gtgtacgcaa taagagtcaa gggtctgaag agctgggtca
    21061 aaagtttctt cgaacccatt tggtggatgt ttccgataaa cataatcggt gaaatcgcaa
    21121 aaccgatttc tcatgcgctc aggctcttcg ggaacataat gggaggggca attctagtac
    21181 tgattcttgg ttaccttgtc aaatactttg ttttgccggc agctctgtgg ggaatattcg
    21241 ggcttttctt cggggcgata caggcgttga tatttacgat actcgcaatt gcatatatta
    21301 gctcattaat agagtaagga ggttttactg tggatattgg tgaaggcttg atgatgatgg
    21361 gaaagtttat tggcgctgga cttgcgatgg gtattggggc tatcggaccg ggaatcggtg
    21421 aaggtagagt cggtgcgagt gcgatggaag caatggcgcg acaacctgaa atgataggaa
    21481 ccctgacaac aagaatgatt ctcgcagacg cgattgcaga gactaccggc atttattcgc
    21541 tcgttattgc gtttatgatt ctcctggtag tgtgaaatct tcccctgact agaggagggg
    21601 tgataacttg attgaactga atttgacagc catattgtcg atactgaact ttttgctgct
    21661 ctttttcgtt ctgaagaagc ttcttttcga caagttcttc gatatcatga agcagcgaaa
    21721 agagaaaatc agcggtgaaa ttgccgaggc cgaaaagctg agaactgaag caaacgacct
    21781 caagagagaa tacgaggcga agattcaaga ggcgcgatca tcctccaatg agataatctc
    21841 acaggccgag agacaggcag aggaaatcgt gagacaggcc agagaaggag cgcagcagga
    21901 aatacagagg atgtacaagg ctgcagaact gcagattcaa cgggagcgag aagaggccgc
    21961 cggtgaaatt aagggtgcga tagtaactgc tgcagttgcc atggtgggga aatttctcca
    22021 gaaggatatg gacgaggctg cgagaaaaca gtacgccagg agaattcttg agagcatggg
    22081 ggataaggag tgaagagaat tctttcgctg gcttcgaagt atgctaacgc tcttttcgat
    22141 agtctgcctt cagaaaaccg agagacagct ctttctcaac ttcagcaact ctctcagagc
    22201 atctcgaacg aggagctctc gaagtttctt ttcgatccca cagttcaggg tgaaaaaaag
    22261 acgggaatac tcctgagttt tgtccaaaga cctctgaagc agatcgaaag attggcagcg
    22321 cttctggttt cgatgaagag gcttgagctc cttcccgaca tcgagaacgc tttttccgca
    22381 aggctcctag ttgaaggcag aatgataaat gttgaggtcg attctgcagt tgaacttact
    22441 tcagaagagg aagaggccat actggagaag gtaagagaaa aaacagggat gcagggtttg
    22501 ttgaaagtaa ctgtggatcc ttcgttgatt gcgggatacg taattagatt cagtgatgag
    22561 gtaatcgaca ctagtctcag tggtcgttta aaaagagtga gccaggagct gcgctcgggc
    22621 cctcgggagg tgattgagtg aagatcagtc catctgagat cacgaaagta atacagaatc
    22681 agctagagaa gactgacata gagtttgatt attttgaagc cggaaaggta attcagatcg
    22741 gcgacggcat agcgagagcc tatggtctga aaggggttat ggcaaacgaa cttgtcaagt
    22801 tcgataacgg tgtctacggt cttgcactga accttgaaga ggataacgtt ggtatcgtta
    22861 ttcttggggg atacaaagag ataaaagaag gagacctggt tcgaagaacg gccagaatta
    22921 tggaagtccc tacgggaccc gagcttctcg gtagagtggt taatcccctg ggaattccac
    22981 tggatggaaa gggacagatc gatacgaaga gcttcaggcc tgtcgacgta aaggcaacag
    23041 gtgtcgtcat gagaaagcca gtagacaccc ctcttcagac gggaataaag gcgatagatg
    23101 ctacgatccc aattggccgt ggccagagag agctaattat tggagacaga cagacaggta
    23161 agacagcaat agctatcgat acgattatca atcagaaagg tcagaacgtt aagtgcatat
    23221 atgttgctat cggccagaaa acggcagccc ttgcaaggat tatcgacaag cttaatcagt
    23281 ttggggcaat ggaatataca acgatagtct tcgcttcagc ttcagacact gccgcgcttt
    23341 cttatctggc accctatgca ggagctgcga tgggcgaaca cttcatgttc gaaggacagg
    23401 attcgctcgt gatctatgac gatctttcaa aacacgcatc tgcttacaga gaactatctc
    23461 tacttctcag aaggccgcct ggaagagaag cctatcccgg agatgtcttt tacctgcatt
    23521 caagattgct tgagagagcc gccagactca acgagaattt cggcggtggc tctttgactg
    23581 ccctgccgat aattgagaca caggcaaatg acgtttccgc atacataccg acaaacgtca
    23641 tatcaattac ggatgggcag atctacctgg atcccaacct cttctatgcc ggtaacaggc
    23701 ccgcaataaa cgttggattg tcggtttcaa gggtaggggg cgctgcgcag atcaaagcca
    23761 tgaagaagat tgccggcagt ttgagaattg agctggccca gtatagagac ttgctttcct
    23821 ttgcacagtt cgctacggaa ctcgacaagg caaccagaga ccagttgacg aggggagaaa
    23881 agctaaccga gcttctcaag caagaccagt acgtgccgat gccggtagaa gagcaagtag
    23941 tcgttctcta tgccggcacg aaaggttacc ttgacgacct tgatacttcc agggtatcga
    24001 cattcgagac tactcttttg aaaaccttgc gagcttccta ttcgaatgtt ttggcttcca
    24061 taagagagaa gaaggtcatc gaagaagaga cggagaagct tttgaagaag gctatcgagg
    24121 aaaccttgaa actctttgca gagcgaggat gattgagcgt gagcaagggt aaactgcgtg
    24181 cgataaagaa acgcatcgag tcaacaaact cgacgatgca gattacccgc gccatggaga
    24241 tggtcgcgcg ggcaaagatc aacaagattc aaaagggact gaaatttgtg aggctctacg
    24301 aaagggctat ggagagagcc ttaatcagag cttactttgg tgatacaagc cacccgaaga
    24361 ttgagggcga aggagagatt cttctggtaa tcagttcgga tatgggcctt gcgggcgctt
    24421 tcaacgccga aataatgaaa gccgccgaac gggagataac taactcagat atcaggcaca
    24481 ttgtgacggt aggcatcaag gccgaaagct ttttcaagaa gggtgacata cctatcagtt
    24541 ctttcagcca tttctacgac actcccgatc tggacgaagc ggccataata gtcgatgatg
    24601 ctcttgacat tatggagagc gagcacgctg ggggtctaag gatggtctac agcagtttca
    24661 agaaccctct ggcgcaaata ccgaaagctg tgaagcttct tccccttgat gaattcagca
    24721 ggcccgacaa cgatttcttt gactttgagc cagagatcga tgtattgttc aaggacgtcc
    24781 tgaattcctg gctggttgca aaagttcttc ttggcatgta tgaaacaaag gtcggagaac
    24841 tattttcaag acaaaatgca atgaagaacg cgacggaaaa cgctaagaac atgattcagc
    24901 agttgacact ggtcagaaat aaactgcgtc agtcgaatat tactcaagag ataattgagg
    24961 tcgttaacgg cgctgaagca ctaaacggtt aattgatttt ggaggtgtga gtgtgcccga
    25021 caaaaataaa cagaacattg gcaaggtcat tgcaataacc ggacctgtcg tggacatatc
    25081 gttcgaggga gcaatgctac ccgacattct taatgctctg aaagtgaaga acaaattctt
    25141 aaataaagag atagtccttg aagttgccca gttgataggt gacaacagcg taagggcgat
    25201 agcaatggac agcaccgatg gattggtaag ggggcaggaa gtcattgata cgggaagcca
    25261 ggtgacggtt cctgtgggag aagagattct tggtagaatg ttcaatctac tcggtgaacc
    25321 gatagatgag aagggtgagg tgaagttttc cgatcactgg gaaatacaca gagcggctcc
    25381 gtcggtcacc gatcagcaga ctgaagtcga gatacttgaa acgggaataa aggtcatcga
    25441 tcttctcgca cccttcagca aaggtgggaa gattggcttc tcgggaggag cgggagtcgg
    25501 aaaaaccgtc ctcgtcatgg aactcataag aaacttcgcg atcgagcaga agggtctatc
    25561 ggtctttgcg ggagtcggtg agagaacgag agagggaaac gacttgtggc tggatatgca
    25621 ggaggccggc gttatcgaga acaccgctct tgtcttcggg cagatgaatg aaccacccgg
    25681 agcgagattc agggtaggtc tctccgccat aacgatggca gagtatttca gagatgtaca
    25741 gaagaaggac gtacttgtat tcatagacaa tattttccga ttcgttcagg ccggttctga
    25801 agtctcggca cttctcggaa gaatgccgtc cgccgtcggt taccagccga cactcgcgac
    25861 agaaatggga aaccttcagg agagaattac ctcgacaaga tcgggttcta tcacttcggt
    25921 tcaggcaatt tacgttccgg ccgatgattt cacggatccg gcgccagcca ctacattcag
    25981 tcatctcgac gccacggtaa acctttctcg ttcgatagcc gaacaggggc tttatccagc
    26041 cgtcgatccg ctcgactcca cctcaaaaaa tctcgatcca gtcgttgtag gtcaagaaca
    26101 ttatcaggtc gcaagaaggg tccaggaggt tcttcagaga tataaggacc ttcaagacat
    26161 tatagcgatt ctaggaatgg aagaactctc cgaggaagac cagcttactg tccaaagagc
    26221 gagaaagatc cagagattcc tgacccagcc attctttgtt gctgaaaaat ttagtaacat
    26281 ggagggtaaa tacgttccca tctccgaaac aatcaggggc ttcaaagaga ttctagaagg
    26341 caaacacgat tccctgcctg aacaggcatt catgatggtt gggactatcg acgaggcaaa
    26401 agaaaaggca aagaatctta cgaaggatga gtgaagtggc cttaaaaatc agaattataa
    26461 ctccctacgg aatagaatgg gagggcgatg cagattttat ctcgttccgg tcgacagaag
    26521 gtgagatagg ttatctgccc gaaagggccc ctgcattgat ggaactgacc gttgatgtaa
    26581 tgaaagtacg gtccggctct aacactcttc agtttgccgt tcacggtggc tatgtccttc
    26641 aggaaagaga aggacttaca attgtgacag atgctgcgga aactccggaa gaaatcagtg
    26701 ttgagcgtgc gaagcagcga ctgaaaaggg cagaggagtt gctccaggta agcaagtcaa
    26761 ggcgcgaacg cgcccgaaac gaggccaaac ttcagaggca catgttgaga atcaagattg
    26821 gttcgagggg ataagttgcc cgtctaagat tgtgaacccc tccttcgaaa gagcagttac
    26881 atgttgtttg ttccaagttg caaaccaaag aataaaaaga agtcaaatgc cgtcatacct
    26941 aaattgcttc ggccccttgg tagccttcgt gaaaccggca cgtcccgtcg tatttcagca
    27001 tctagtttct ccaaagagaa aacggagaaa atccattgtc gagagttcgc ttcgctcacg
    27061 agaaggcgag acttctcgcg tgttttcaat ctctttttct ctctcttcct ctcttcttct
    27121 ct
//
LOCUS       DAOQYI010000026        23447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_2526_length_23447_cov_82.618203, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000026 DAOQYI010000000
VERSION     DAOQYI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 23447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23447
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2526_length_23447_cov_82.618203"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKL93_07590"
     CDS             complement(<1..401)
                     /locus_tag="PKL93_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71157.1"
                     /translation="MRIGDMKSSFSEFWQEFRRVKSGMIGLVFLGLFLMVLFFEPLLL
                     PFEGANQRWKDITYWEDNPASAAPQWTNLFSSKKSAVSIALESAKKEEKNAGPIRIIE
                     ETFVYDFQYDVAPSDIIFRASGKANPTAIIS"
     gene            complement(398..1438)
                     /locus_tag="PKL93_07595"
     CDS             complement(398..1438)
                     /locus_tag="PKL93_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71158.1"
                     /translation="MFWKYMLKRVFYGIFIYIILIFVFSALFNTVMEQTLRGQIDEEI
                     RGELMALDNRSSQQIQDFIEMRRAEKYALYRLDKPIFERIVWRTWSTLTFNLGKSSSI
                     RSAAGDRDVWSIVSEKIPRTLVLFSTAMLVDIFIGIWLGLKKAQKAGGLLDKSTSVGT
                     MVVFGMPSWWLGMILIMFFAYTLKVFPSGGLHATPPPEGIAFFLDALYHLALPVMTLV
                     VIGFWGRAFLTRNIVLGVLQDDYIMAARARGIPERKVLYGHTMRTSAPPIVTMSLLAL
                     LASVSGNIVFEGIFNWPGMGNLFWFALQQNDVPVLMGNLAITTGLYICGLVLLDLVYG
                     LLDPRIKVGGRI"
     gene            complement(1491..3992)
                     /locus_tag="PKL93_07600"
     CDS             complement(1491..3992)
                     /locus_tag="PKL93_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71159.1"
                     /translation="MSKRLTVILLASMIVLCGGVFATEKGPISDVIYFNVKMSQELGL
                     RDVAEGLTDIFMEGVSGPTLMGMDKATRDKMDIYSVPALTYALFVNPYPNEAPYFANV
                     DGKDFFNVMAINEFRYALNDLINRQYIVDEILGGAGGPMLSMATPGQPGTYRYGLVAN
                     KLGLSFEGNETRALEAMKKALEDAAKIPENQGRLVNSNGKWYFDNEPLTVKFVIRADD
                     PNVRVKMAEYVSLQLEKAGVTVDRLVWERSKASNTAYGSDPADLIWNLYTEAWSAGAT
                     RAFWEHIVRQMYASGGSYMPGRGTEGFWNFRNEKLDDLIDRAYTGNFLTEEEYWDLSL
                     TALEMGLKDAVRIYVCYQDGFYTANSARLLDRFAYGLGDGVNDWSLKTAVTDDKVLFV
                     TQYSSVGALFMSNWDPVGPDGISDTYSANVVTILSDPSSFESPASAINTPYRVNWTHD
                     DIVTKVERNSEGDVVGTIDVPENAIRFDSKTKQWKEVGPGVKSMSMGTYTLLGGNMHQ
                     GQPITVNDYLYAFAFIDEWISRDGENDKYYEASYENYMRSGQDTIKGYIVNDDGTITT
                     YFDYNFPASVDRTAQWGAPFLGVHRQPFVIVSWEIYEALAEIVANGSQSGTVYAFRND
                     PAVTQIDVLVPNHVKDIKAKLVELIEKGHVPNSLKGLITVEEAQSRYQAAIDFIDKYG
                     HAYISNGPYYLSKYDPTANYAECRAFRDPSYPYEPYYWKEMLKTVRLSIDSIDVPAII
                     MKGMDTTVKVVVAEVAYPSEEVSKASRGSVVVTLITPDKEMTFDAILIEPGLFEAVIN
                     AEAIEDLEPGSYNVLAIASLEGAVPASSVTSTIVY"
     gene            complement(4409..5311)
                     /locus_tag="PKL93_07605"
     CDS             complement(4409..5311)
                     /locus_tag="PKL93_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ71160.1"
                     /translation="MTVIILVLIVVGFLGIVGAVFANKVTKPRQIPYEETYRIEVENG
                     RMDPEWFEKIEKEEVWIDSPYGYKLHGLLIPNDNSERAVIICHGITYSLFGSIKYAKI
                     FHKLGFNIIVYDHRNHGKSGGTNTTLGYYEKHDLAAVKNWVLDRLGKKTRIGLHGESM
                     GAAIAIQYLSLDDEIDFCVADCGFSDLEELLSIRLREDFHLPRVPFIWLARLFARIMT
                     GADLKEVSPIRSVRETSIPIMFAHGGEDHYVPTFMSEKMYSERQSNKHLLIVPDAGHA
                     MALVTDPAKYEKEIEWFLRSNDLL"
     gene            complement(5331..5612)
                     /locus_tag="PKL93_07610"
     CDS             complement(5331..5612)
                     /locus_tag="PKL93_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNQ71161.1"
                     /translation="MRTVEIIIQGRVQKVGMRNFIRRLATRHEITGYVENLDDGSVRV
                     VAQGSKAQLNAFLAKIEKGSISIKLSGGNIKRVTERPSMEHGFMDFEKR"
     gene            5864..6340
                     /locus_tag="PKL93_07615"
     CDS             5864..6340
                     /locus_tag="PKL93_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71162.1"
                     /translation="MIVIEKMRSDEFSVYLRKLVENYAVENVKSGRWEEKSALEKSKR
                     EVDSLLTDGLNTENHELLNLKEKDTGKKIGCVWLHIFPGMEKKGFIYDFVIDEAFRGK
                     SYGKESLKALEEYASKLGVEDLSLHVFAHNTVAVALYRKMGYEVTSLNMAKHIGGK"
     gene            complement(6368..6443)
                     /locus_tag="PKL93_07620"
     tRNA            complement(6368..6443)
                     /locus_tag="PKL93_07620"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6408..6410),aa:Trp,seq:cca)
     gene            complement(6478..7920)
                     /locus_tag="PKL93_07625"
     CDS             complement(6478..7920)
                     /locus_tag="PKL93_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNQ71163.1"
                     /translation="MIALVFLPVLGGVICLVLKKRKNVSLIIAIVTALVGGSVLPFVI
                     FDNASIDIGNWGSLGIGLAIDELAIPFLLSTFIVMLAVILNSIKKGYDGFFYALILIL
                     YGTLNSIFLSRDLFSIFVTVELASIISFILVSYEKKPRQAWASLKYLLLSSLGLNFYL
                     LGIGIVYMETGSFAMDSLEQVSTIATVLIFGGLAVKSGLFFFSMWLPDAHSNAPIEVS
                     PILSGLIVKLDVYLSIRFITYSSFGWMRDTYMFIGIVSAIVGVVFAVNSKNAKRLLAY
                     HTISQVGFMLVNCDKSSAWHGFSHAIFKTLLFLVVGNISERLGTKEYTKWSGKITRVE
                     YAFLLIGSLAIAGFPMTSGCVTKEIIIHGACCPSLKILLLIASAGTAMSFSKFIFLKP
                     GKSSSRPAANTVAAYSILSGVIIVHGIIGFEIYMFESLFAVIAGVAAYLLLRKFLRPL
                     PVYFERIDSALSSYLVLFLISIVLAIILSS"
     gene            complement(7917..8276)
                     /locus_tag="PKL93_07630"
     CDS             complement(7917..8276)
                     /locus_tag="PKL93_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HNQ71164.1"
                     /translation="MTGILVIYFSIIGMLVGAVGMILSKNIIKKILSLGIVETSVNLM
                     YTGISARGGIIPPIISGDFSNANYADPIPQAVIITGIVISFALLCLSLVFAIIIYNKY
                     HTMNVETIEMIFEREEK"
     gene            complement(8273..8944)
                     /locus_tag="PKL93_07635"
     CDS             complement(8273..8944)
                     /locus_tag="PKL93_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNQ71165.1"
                     /translation="MRIAGIIALIAIFMIVLFSFDPFGSIPEPIEEIVNRVNVPNLVT
                     SVYLETRLYDTIFEIIVFTITALGVASLFTSLPVNSDEGQQVFGTVTVYSGGLAALSI
                     TLFLYVVINGHISPGGGFVGGVVLATGVVTYGLTSSFRRASLHYEIFKVNILENVSLI
                     LIFAFSCFIMIFPEAHAQMLSEAGFGSVFSGGLIPMMNILIGIKVYAGAWKMSSEFID
                     RRGTL"
     gene            complement(8941..9630)
                     /locus_tag="PKL93_07640"
     CDS             complement(8941..9630)
                     /locus_tag="PKL93_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNQ71166.1"
                     /translation="MSFVIVAISSLYIVSAIIVLSSKTNFQSVIWFGIVGSLSAIIMM
                     IIGAPDVAMTQFSVGVALVLIVYIMALKKQRRVRLGIVEVPSMIEETPSGFRGLEWEI
                     IQLVDEKEGYHLEPVKFSSKEEALIAIENHDIDLICGALTEDDVAGRTKGIPYLETSI
                     FLCNGEEIDFVRLKHLSRNSLSPTPEFLRKSSYVFVVSTNSPDLEKDILEGLEFIKSS
                     GNIEEIVGRYL"
     gene            complement(9627..9908)
                     /locus_tag="PKL93_07645"
     CDS             complement(9627..9908)
                     /locus_tag="PKL93_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNQ71167.1"
                     /translation="MMEIIAYIILIPGLFFVVAGTLRAVFAHTIIGTLHFLTVADTVG
                     LIFLVISAFFFGLLTIIEMLAFIVALMVTGPLVTHVIARAFINSGGREK"
     gene            complement(9905..10153)
                     /locus_tag="PKL93_07650"
     CDS             complement(9905..10153)
                     /locus_tag="PKL93_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71168.1"
                     /translation="MTFYITMAATISSIVMLVWFAAGSNPWRLLVAYSAISTRLLIGI
                     IFIEMVTSVDFVSSVALLFLILNTSGTIIAAYYMGVRR"
     gene            complement(10150..10503)
                     /locus_tag="PKL93_07655"
     CDS             complement(10150..10503)
                     /locus_tag="PKL93_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71169.1"
                     /translation="MIFTISFFLWITFFGRFTLASVVSGLLVSVLPQYISSRLIRSGP
                     VFSTAFKIILALPIAVFQAFRLIFSRPIFTVRSEKSPENRIVEFGKIISITMTPEEIV
                     ISKDREGLLIHEVKK"
     gene            complement(10504..11301)
                     /locus_tag="PKL93_07660"
     CDS             complement(10504..11301)
                     /locus_tag="PKL93_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ71170.1"
                     /translation="MQKIEPRVSATVLHFGEEPPLVKEGGAGAIFFSGCSMSCLYCQN
                     FAFSQKNSGKVFSFEELSRHFMKIQEEGAACIDLVTPTPNLYGFLKAYELALKEGFSL
                     PVVFNTSGYEEVSTLRLLDGIADIYLTDIRYTDDEVGKKYSLVPDYWTVTKKAVKEMF
                     RQTGSFREENMRGVIVRHLVLPEGIAGTEEMAEFVAFELSSSVPISLMSQYRPVYRAR
                     EYYELSRRISEEEYDHALNVIDAFGLTGWMQHFESNEGYRAKPLRWD"
     gene            complement(12252..13520)
                     /gene="murA"
                     /locus_tag="PKL93_07665"
     CDS             complement(12252..13520)
                     /gene="murA"
                     /locus_tag="PKL93_07665"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNQ71171.1"
                     /translation="MSQLVVMGNTSLKGEIKASGSKNSVLPILAATVMIEEPVVIKNV
                     PELRDVQTMISILQQIGKTVSFENETVTVMPGEILVGNVPYELVKKMRASFNIIGPLA
                     MICGWAKVGKPGGCNIGQRPVDFHLKGLEAFGFRITEEHGDVMAVIPNSFEKEIVYAL
                     PFPSVGATEQLMTTAALMEGSKITIENAAREPEISDLQNFLNACGAQIAGASTPTIKI
                     TGVRNLHGSEYSVIPDRIETGTYLLGTIATKGDVKIHGTRSDHLISLLSILEEMGAGI
                     EKGQDYLHAFWKGPLKPVRISSEPYPGFPTDLQPIITAVLATVEGTSVIEENVFENRF
                     GYVDELNRMGARIKVSSSHANICGVDKLSGAEVVAPDIRAGAALVIAGLAADGETIIN
                     NVTHLFRGYERFQEKLRTLGAKITFYPDEE"
     gene            complement(13830..14180)
                     /locus_tag="PKL93_07670"
     CDS             complement(13830..14180)
                     /locus_tag="PKL93_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71172.1"
                     /translation="MTTGRIDASPASRVALGWFVKEIEGVKVLQHGCRVNGQISLLAV
                     LLDLNLSAVLTNSNDGSKATSIFTRMVIEKLLGLSPIILVSASDELESRKDRWEVFGR
                     SPTWISSKKTANCS"
     gene            complement(14383..14883)
                     /locus_tag="PKL93_07675"
     CDS             complement(14383..14883)
                     /locus_tag="PKL93_07675"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNQ71173.1"
                     /translation="MCFGETVDTASYGVTNLSNPLKVNEDNFFQISSITKTFVALAVM
                     RLADRGRVDLERPLLDYLPNFKLSDGETEKRLTMKHLLIQLSGKGDYFKDFRFGKNVM
                     GNMVKITLTLPQINPPGNDYFVLQFRFHVASRVIEAITGKPFEEAINELVSKPLDLDK
                     IRFSPE"
     gene            15072..16340
                     /locus_tag="PKL93_07680"
     CDS             15072..16340
                     /locus_tag="PKL93_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ71174.1"
                     /translation="MAKLNLSDLLIPMSKRVKFEEKIGLVLSGGGARGAYQIGVWKAL
                     KDCGIEIGGVYGTSVGAINSMAVAMDNFEDARQLWLKIDFDKVVKNGPDGNLIEKALA
                     ALKSRGFDPAPLRENFGRLLKEELVRKAKIDVGIVTYSLSNMEPKELFLDDIPEGKLE
                     DYILASANHPVFKREEIGSEKFIDGGIYRNIPVNMALSRGFKEIIIVDLGPKRIRDVL
                     TLTSLKRLEEVKYLVIKPREFYGDVLDFDPEIARNFMREGYLNCLDDLGYLNGEEYFV
                     FASHDTIARALFSLSKKKKEEARAIFAVEPWQSESTHHFYYGQLMPVLQDHFGIFNPV
                     ETWVRLLEDLARYLELERLDLYSLYSLTKEILSSRNVGVEGRNYNDVSCESILNFLEF
                     LVKNSDLESLEDREFSLFREGFLSLTDLND"
     gene            complement(16390..16980)
                     /locus_tag="PKL93_07685"
     CDS             complement(16390..16980)
                     /locus_tag="PKL93_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNQ71175.1"
                     /translation="MNLKGSETVENLMKAFAGESQARNRYTFYASVAKKEGFEQISAI
                     FKETADNEKEHAEVFFKHIIDGMKDELPVMVHVDADYPVDLRGTRENLKAAAAGENEE
                     WTKLYPTFADVAEKEGFLDVANSFRQIAKVESRHEARYLKLLENVETGKVFKREEKVL
                     WKCGNCGYILEAKEAPEQCPACKHPKAYFELFVENY"
     gene            complement(17134..18093)
                     /locus_tag="PKL93_07690"
     CDS             complement(17134..18093)
                     /locus_tag="PKL93_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNQ71176.1"
                     /translation="MTEDRVALSRRGSIIGVTGNAALAVLKVLTGFFTGSMAILADGV
                     DSTTDILTSVITWISTSVSNKPPDAQHPYGHERADAVASKIVSMVIFFAGAQLAVSSI
                     QKLFSGGSSVENIALVILVAAISSAAKYFLYRYKLGIGKKIDSSVFIADATNMKLDIV
                     ISLSVLGGSIFVGITGLQIVDSIVGLAVSALVIKTSIEVFWETNYELMDGLKPGDDIY
                     RVVFDSIERVQGVQNPHKVRVRKMGYKYLVDLDIEVDPDMSVKKAHSLAKKVEASIKE
                     DHKNVYDVHVHVEPSGNVEDENFGIDSSKEFNESLNNKDKKSE"
     gene            complement(18090..19526)
                     /locus_tag="PKL93_07695"
     CDS             complement(18090..19526)
                     /locus_tag="PKL93_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ71177.1"
                     /translation="MFYELYLRSFCDGNGDGIGDFIGAERKLDYLADLGIEGIWLLPI
                     LHSPSYHGYSVIDFFSVNPIYGTLKDLRNFLASAHKLDLKVILDLPMNHTSPNHEWFL
                     KALDGEKPYRDWYLFLKDEQWLKARRHWDGEQVWTNYSGQWVYTLFGPGSPDLNYESS
                     SLWQQMKEVLTFWLSVGFDGFRFDAAKHIFDFSLEKGICEYQHSRNIAFWKEMTAHCR
                     SISPNSIFVSEVWDDAQIVDLYSTIFGIGFNFPLAEDLKTSVATRDPELLVKSLRTDL
                     NRYFRSKRSYESGIFLTNHDMSRLVSAMDGDKELSLLAMSVLLSLPGVPFFYYGEELG
                     MEGVYNAYFNEEQLEPFLWFENGYGPGQTEWKALGKNHPHSGRSLEAQKAVDGSYFER
                     IRAILEFRKNNSWLRLATIRGLRRRENLVILRVVGESRNAFIYHNLGKKRVALEDVSS
                     LQIINGSLSRYRGKRYLGGFSSAIEVTK"
     gene            19668..20717
                     /locus_tag="PKL93_07700"
     CDS             19668..20717
                     /locus_tag="PKL93_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNQ71178.1"
                     /translation="MLIKEILSKGEQMNASDIHLTSGENVIYRVDGELIQDKLLTDEI
                     MMKKITTELQEISEIKLQDSESKEIDFSFGFSSLRIRGNYFYSNKLPVVALRLIPKTI
                     KTLEDLGYPPLFKEFCKPDKGLVLVAGPTGSGKSTTLAAMLENINRTRHVHLITIEDP
                     VEYVFEPKNALIHQRELGSDTKSFYNGLKYALRQDPDVILVGEMRDSETMELAMTAAE
                     TGHLVFSTIHTNSAATTPERIVGVFPPHQQNQVAMQLANTLLAVIYQRLLRRRDGKGR
                     IAVLEIMVVNQAIRNLIREQKFHQIESMMQAGIKYGMVTFDDALMDVFKKGIIDKDQL
                     QDYARDPNAMLRRAF"
     gene            20717..21799
                     /gene="prfA"
                     /locus_tag="PKL93_07705"
     CDS             20717..21799
                     /gene="prfA"
                     /locus_tag="PKL93_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNQ71179.1"
                     /translation="MDLGKIMEVFRERFKEVEKELSKPEVASDPDKLMEYGKKHAELS
                     EIINLYSEIDSMKGELEEWHEMKATAEENEQEVIANTISDLENRIARKELNLKGLLVP
                     DLADERNIIIEIRAGTGGEEAALFAADLFRMYTRYAERNNWKSEIIDSNETDIGGFKE
                     VIFQIRGRSVFSKLKYESGVHRVQRVPTTESGGRIHTSTATVAVLPEVSETEIKIDPA
                     DLRIDTYRSSGAGGQHVNKTDSAVRIVHEPTGIVVAVQKERSQHQNKARALEILRARL
                     YEMYSSKAENEITEARRTQIGTGERSEKIRTYNFPQNRVTDHRINFTSYRLLYVMDGD
                     LDELVTELSAADINKRLELLHKKLIG"
     gene            21828..22331
                     /gene="ybaK"
                     /locus_tag="PKL93_07710"
     CDS             21828..22331
                     /gene="ybaK"
                     /locus_tag="PKL93_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNQ71180.1"
                     /translation="MKSRKPLKTNATRILDGLGIEYEILEYEVDEHDLSAENVASKLG
                     LSPSRTVKTIVLKGDRSGVFVCCLGGHREIDMKKLPEITGDGNIETVPSESLLTLTGY
                     VRGGVSPLGMKKSFKILVDKGVLNEERISVSAGKRGLQIWLRPTDLVCATNASVVSFS
                     KEKHQTL"
     gene            22591..23076
                     /locus_tag="PKL93_07715"
     CDS             22591..23076
                     /locus_tag="PKL93_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNQ71181.1"
                     /translation="MRTIIKIVGGIVYVVGIGLIIFSFASFFLAPYLSLVFGGVIVFL
                     VLGFIATTLGRMLLAMAKTKSFSGPVSEQETVSGRELKEDMTYDYTFVSDREARIEKT
                     KSKSYSRCPECGAENEDNATKCSNCGASLVGYKKCSLCYMLNKKENRFCKNCGHDFTL
                     D"
     gene            complement(23173..>23447)
                     /locus_tag="PKL93_07720"
     CDS             complement(23173..>23447)
                     /locus_tag="PKL93_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71182.1"
                     /translation="EALLFRIWVFSGTVVGGRRLVVGKSEVKPVLRWTVNRPPNKTSS
                     FLVKAKKREILNGVQNDGMRRFEIFIHCHPELVSGSGFDPLGGRNP"
BASE COUNT         6485 a         5389 c         5270 g         6303 t
ORIGIN      
        1 atggatatga ttgctgtcgg attcgcctta ccagaggctc tgaatattat gtcggaggga
       61 gcgacatcat actggaagtc atatacgaag gtctcctcta taattcttat cgggccggcg
      121 tttttctctt ccttttttgc tgactcaaga gctatggata ctgcgctctt ctttgaggag
      181 aataggtttg tccactgagg tgcggcactg gccggattat cctcccagta ggttatgtcc
      241 ttccaccgtt ggtttgctcc ttcaaaaggt agaagcagcg gttcaaaaaa caaaaccatt
      301 agaaacaatc caagaaagac aagaccaatc atccccgatt tgacccttct aaattcttgc
      361 cagaactcgc tgaatgatga tttcatatct ccaattctca tattctgcca ccaaccttga
      421 tcctgggatc aagaagaccg tacaccagat cgaggagaac aagaccgcaa atatacaatc
      481 cagtggttat cgcgagattt cccataagga ccggaacatc gttctgctga agggcaaacc
      541 agaagagatt ccccatccct ggccagttga agataccttc aaagacaata tttcctgaca
      601 cagaggcaag cagtgcaaga agcgacatgg tgacgattgg cggggccgat gttctcattg
      661 tgtgtccgta gagaaccttc ctttcgggga tgccccttgc tcttgccgcc attatatagt
      721 cgtcctgaag aactccgaga acgatatttc gagtgaggaa ggcgcgcccc caaaatccaa
      781 ttacgacaag agtcatcact ggaagcgcca aatgatagag agcatctaag aagaatgcta
      841 ttccctctgg aggaggcgta gcgtgaagtc caccagaagg aaatactttt agtgtgtatg
      901 cgaagaacat gatcagtatc atgcctaacc accacgatgg cataccgaag accaccatgg
      961 ttcctactga agtacttttg tccagtaacc ctccggcctt ttgagccttc ttcaggccaa
     1021 gccatattcc aataaagatg tcgacgagca tcgctgtgct gaaaagaaca agggttcttg
     1081 gtatcttctc cgaaactatc gaccagacat cccgatcgcc tgctgcagag cgtatggaac
     1141 tcgattttcc aaggttgaag gtcagggttg accaggttcg ccacactatc ctttcgaaga
     1201 tgggcttgtc cagtctgtaa agggcatact tttctgccct tctcatttcg atgaagtctt
     1261 gaatctgttg ggagcttcgg ttatctaggg ccatcaattc gcctctaatt tcctcatcga
     1321 tctgtcctcg caaggtctgt tccatgacgg tgttgaaaag cgcagaaaag acgaaaatca
     1381 gtatgatgta gataaagatg ccgtagaaga cacgtttcaa catgtatttc cagaacatat
     1441 tccacctcaa taatgaaagc ggcacccttt cgggtaccgc tctgcagaca tcagtaaact
     1501 atggtggaag tgacggacga tgccggcacc gcaccttcga gtgaagcgat tgccagaacg
     1561 ttgtatgagc caggttcgag atcttcgata gcttcagcgt ttattacagc ttcgaaaagt
     1621 cccggttcga tcaagatggc gtcgaaagtc atctctttat ccggagttat aagtgtcacg
     1681 acaacgcttc ccctggatgc cttggatacc tcttcagatg gataggcaac ctctgcgact
     1741 actaccttga cagtcgtatc cattcccttc atgattatgg ccggtacgtc tatagagtcg
     1801 attgaaagcc ttacggtttt caacatttcc ttccagtagt acggttcata tggataggaa
     1861 ggatctctaa aggccctgca ttccgcatag ttcgcagttg ggtcgtattt tgaaaggtag
     1921 tatggtccat tgcttatata cgcatgtcca tacttgtcta tgaagtcgat tgcggcctgg
     1981 tatcttgact gagcctcttc gacagttatc aggcccttaa gagagttagg aacatgtccc
     2041 ttttctatta gctcgacgag tttcgctttt atgtctttca cgtggttcgg tacaaggaca
     2101 tcgatctgag tgacagcggg atcgttcctg aaggcataga ccgttccaga ttggctgccg
     2161 ttggcaacga tttccgcgag ggcctcgtat atttcccagg agactattac aaatggctgt
     2221 ctgtggactc caaggaaagg tgctccccac tgtgcggttc tgtcgacgct tgcagggaag
     2281 ttgtaatcga agtaggtagt gattgtcccg tcatcgttta caatatatcc ctttatcgtg
     2341 tcttgtccgc ttctcatgta attctcgtac gaagcttcgt agtacttgtc attctccccg
     2401 tctctcgata tccattcatc aatgaatgcg aaggcgtaga gatagtcgtt gactgttatg
     2461 ggctggccct ggtgcatgtt gcctccgagg agggtatatg ttcccatcga cattgacttg
     2521 acgcccgggc cgacttcctt ccactgcttg gtcttgctgt cgaatcttat cgcattttcg
     2581 ggaacgtcta ttgtacctac gacgtcacct tccgagtttc gctcgacctt cgtgactatg
     2641 tcgtcatgtg tccagttaac tctgtaaggg gtatttatag cagacgccgg gctctcaaaa
     2701 gagctgggat cagagagaat cgttacgaca tttgcactgt acgtgtctga gattccgtca
     2761 ggtcctacag gatcccagtt ggacatgaag agagctccaa cggatgagta ctgtgtgaca
     2821 aaaagcacct tgtcatcggt gacggccgtt ttgagtgacc agtcattaac tccgtctccg
     2881 agtccgtatg cgaatctgtc gagaagcctc gccgaattgg ccgtatagaa gccgtcctga
     2941 tagcagacat aaattctaac tgcgtcttta agtcccatct ccagagccgt aagagaaagg
     3001 tcccagtact cctcttcagt taagaagttt cctgtgtagg ccctatctat gagatcgtcc
     3061 agcttttcgt ttctgaaatt ccagaaccct tctgtcccac ggccgggcat gtaggatcca
     3121 ccggaagcat acatctggcg tactatgtgt tcccagaagg ctcttgtggc gcctgcgctc
     3181 catgcttcgg tgtagaggtt ccagatcagg tctgcaggat cgctaccata agctgtgttg
     3241 ctagctttgg atctttccca gaccagtctg tccactgtta ccccggcctt ttcaagttga
     3301 agggagacgt attcggccat cttgactcta acgttggggt catcagccct aataacgaat
     3361 ttaaccgtca atggctcatt atcgaaatac catttgccgt tactgtttac cagtctaccc
     3421 tgattctcag gaatctttgc ggcatcctca agagctttct tcatagcttc gagagctctt
     3481 gtctcgttgc cttcaaagct taacccaagt ttgtttgcga ccagaccgta tctgtaggta
     3541 ccgggttgac cgggggttgc catagacaac atcggtcctc cagcgcctcc aagtatctcg
     3601 tcaactatgt actgcctgtt gatcaagtcg ttgagagcat acctgaattc gtttatcgcc
     3661 attacgttga agaagtcctt tccgtctaca tttgcgaagt aaggagcttc gttcggatat
     3721 gggtttacga aaagagcata ggttagcgct ggaacagagt agatatccat tttgtctcgt
     3781 gttgccttat ccatccccat taacgtagga ccggagacgc cttccatgaa aatgtcggtg
     3841 agtccttcgg caacgtctct gagaccgagt tcttgagaca tcttaacgtt gaagtagatg
     3901 acgtcggaga ttggtccctt ctcggttgca aaaacaccgc cacagagaac aatcatcgat
     3961 gcgagaagga tcacggtaag tctcttactc aatttctttc gccccctttt tcatttagtt
     4021 ctgtgccttt gcacagaata ttcagttgta tatcagtaag aaattttcat tataatcgca
     4081 taattataat tctggacaga actatagtgc aaaaagtaat cgataattta attacttcga
     4141 taaaaagaaa aagattatca aagcattacg aagtctaaca caataattcc aagaatgtca
     4201 agctatttgc ttcaataatt aatggaaaaa taattaatta gttgtagtga ttgattattt
     4261 caatatgaat tagcgtgcaa atcaataaat taaactaacc aaaaccaaat tatcgatata
     4321 gttcggcaag gtaatgaata attccaacaa tggattttaa ttcttaaggc aaatttttca
     4381 atagtgaggg cggaaataaa gagctgaatc aaaggagatc attactacga agaaaccatt
     4441 ctatctcttt ttcgtatttc gctggatcgg taacaagggc catcgcatgg ccagcatcgg
     4501 gaactataag tagatgtttg tttgattgtc tttcactgta catcttttcg ctcataaagg
     4561 tagggacgta gtggtcttct ccaccgtgag caaacatgat aggaatggaa gtctctctta
     4621 ctgatctgat cgggctgact tccttgagat cggctccagt cattatccta gcgaagagac
     4681 gggcgagcca gatgaatgga actcttggca gatgaaagtc ctctctcaac cttatcgaga
     4741 gcaattcttc gaggtccgaa aaaccacagt cggcgacgca gaagtcgatc tcatcatcta
     4801 gagaaagata ctgaattgcg attgcggcac ccatcgattc cccatgcaga cctattctgg
     4861 tcttcttgcc cagtctatcc aggacccagt ttttcaccgc agcaagatca tgtttctcgt
     4921 aatagcccag agtcgtattc gtgcctccgc tctttccgtg atttctgtgg tcatatacga
     4981 tgatgttaaa gcctagcttg tgaaaaattt tcgcgtactt tatggaaccg aacagcgaat
     5041 aagtgatacc gtggcagata attacagctc tttcggaatt gtcgttcgga atgagtaacc
     5101 cgtgaagttt gtatccatac ggagaatcaa tccagacctc ctccttttcg atcttttcga
     5161 accactccgg atccattctg ccgttttcga cttcgatgcg gtaggtctct tcgtacggga
     5221 tttgccgcgg tttcgtgacc ttgttggcaa aaacggcacc gacgatgccc agaaatccta
     5281 caacgatcag caccaatatt atcactgtca tttttctcct ccagtaggaa ttaccttttt
     5341 tcaaagtcca tgaacccgtg ttccatggat ggccgttcag taaccctctt gatgtttcct
     5401 ccggaaagct ttatcgaaat cgatcccttc tcgatcttcg caaggaatgc atttagctgt
     5461 gctttgctgc cctgagctac tactctcacc gagccatcgt cgaggttctc tacataacca
     5521 gtaatctcat gtctcgtagc cagacgcctt atgaagtttc tcatgccgac cttctgaact
     5581 cttccctgaa taattatctc gacggttctc aaactgtgat ttctcccctt gtgatttaga
     5641 atatcatgta taaaacaatt ttatcagcag aaagtgtact tgatcacttt aagaaagcag
     5701 tagtggtttt cattaatcaa ggaagtgtac tttcttgttg ctctttctaa gactggttgt
     5761 tgtcctgaag tttccggtca tcagagtacg tcgacgatta cttttcaatc aagatactct
     5821 tcaagagatt tctgctggat atgatctata gaggagggac agaatgatag ttatagagaa
     5881 aatgaggtca gatgagttta gcgtatatct gagaaaactt gttgaaaact acgcggtaga
     5941 gaatgtgaaa tccggaagat gggaagagaa aagcgcacta gaaaaatcga agagagaggt
     6001 cgattcattg ctgactgacg gactgaacac agagaaccac gaactgctga atcttaagga
     6061 aaaggataca ggcaagaaga tcggctgtgt ctggctacat atcttcccgg gaatggagaa
     6121 gaagggattc atctatgatt tcgtaatcga tgaggcattc aggggaaaaa gctacgggaa
     6181 agagagcttg aaggcgcttg aggaatatgc ttcaaaacta ggagtggaag acctttcgct
     6241 ccatgttttc gcacataaca ccgtggcggt tgctctatat aggaagatgg ggtatgaagt
     6301 taccagcttg aatatggcaa agcatatagg ggggaaataa aaaagagagg aaacttcctc
     6361 tcttttctgg caggcgagga gggcctcgaa cccccgacct acgattttgg agaccgtcgc
     6421 tctgccaatt gagctactcg cctacaaaga cgattatagc ggaatcgata ctccttttca
     6481 agaggagagg ataattgcca gcacgatcga aattagaaat agaacgagat aggaagagag
     6541 agccgaatcg atcctctcga aataaaccgg cagaggtctg agaaacttgc ggagcagaag
     6601 atatgcggct actcccgcaa taacggcaaa taatgattcg aacatatata tttcgaaacc
     6661 gataatcccg tgtacgatta tcactccgga caaaatggag taagccgcaa cggtattcgc
     6721 ggcaggccgc gaggaagatt ttccgggttt gagaaagatg aatttcgaga agctcatggc
     6781 cgttccagca gaagcaatga ggagcaggat ctttaatgag ggacaacaag cgccgtgtat
     6841 aatgatctcc ttcgtcacac atccggatgt cattggaaat cccgcgattg cgagcgaacc
     6901 gatcaacaga aaagcgtact cgaccctcgt gatcttcccc gaccactttg tatactcctt
     6961 tgtaccgagt ctttccgaaa tatttccgac gaccagaaaa aggagcgttt tgaatattgc
     7021 atggctgaag ccgtgccagg cagaggactt gtcacaattc acaagcatga atccaacttg
     7081 agagatagtg tgatatgcta gcaacctctt ggcgttcttt gaattgactg cgaatacgac
     7141 gccgacaatt gcgcttacga ttcctatgaa catataggta tctctcatcc agccgaaaga
     7201 ggaataggtt atgaacctta tcgataaata gacatcaagt ttgacaatga gaccggatag
     7261 tattggagac acttcgattg gcgcattgga atgagcatcc ggaagccaca ttgagaaaaa
     7321 gaaaagaccg gacttgacgg ccagcccccc aaagattaga actgttgcga tagtcgaaac
     7381 ctgttcgaga ctatccatcg caaatgaacc cgtttccata tagacgattc cgattcctag
     7441 tagataaaag ttcagaccta gtgaagagag gagcaaatac tttagacttg cccaggcctg
     7501 acggggcttc ttctcgtagg aaactaggat aaaggaaatt atcgatgcga gttcaacggt
     7561 tacgaaaata ctgaaaaggt ctctcgaaag gaatattgaa tttagagttc cgtaaagtat
     7621 gaggatcaag gcatagaaaa aaccgtcata acccttcttg atagaattca agatcaccgc
     7681 gagcataacg ataaaagtcg atagcagaaa ggggattgct aattcatcta tggcaagtcc
     7741 gattccaagt gaaccccagt tccctatgtc tatcgaagcg ttgtcaaaga taacaaatgg
     7801 aaggacgctt ccaccgacta aagccgtaac aatggcaatg atcaggctga cgttctttct
     7861 cttcttcaat acgaggcaga taacgccacc gagaacgggc aggaagacta atgctatcat
     7921 ttttcctccc tctcgaaaat catttcgata gtctcgacat tcatggtgtg atacttgttg
     7981 taaattatga ttgcaaatac gagcgaaaga cagagcaatg caaatgaaat tacgattccg
     8041 gtgattatta ctgcctgggg aatggggtct gcatagtttg cattagagaa gtcaccgctg
     8101 attatggggg gaattatccc tcctctggcc gaaattccgg tatacataag atttacggag
     8161 gtctctacta taccaaggga caagattttc tttattatgt ttttcgagag aatcatgccc
     8221 actgcgccga ccagcattcc aattatcgag aaatagatta cgagaattcc cgtcataatg
     8281 ttccccgtct gtctatgaac tcgctggaca tcttccaggc gcccgcatat actttgattc
     8341 ctatcagtat attcatcata ggtattagtc caccgctgaa aaccgaaccg aatccagcct
     8401 cagataacat ttgtgcgtga gcctccggaa agatcatgat gaaacatgaa aacgcaaaaa
     8461 taagaataag gcttacgttc tccagtatgt ttaccttgaa gatctcataa tgaagactcg
     8521 ctctcctgaa actcgaagtg agtccataag tgacaacgcc cgttgcgaga actactccac
     8581 caacaaatcc tccaccgggg cttatgtgac cgtttatcac aacatatagg aagagcgtta
     8641 tggacagtgc agccagtcca ccgctgtaaa cagtaaccgt tccgaatacc tgctgaccct
     8701 cgtcgctatt aacgggaagc gaagtaaaaa ggcttgcaac accaagtgca gttatcgtga
     8761 agactattat ctcgaaaata gtgtcataga gtcttgtttc aagataaacg cttgtgacta
     8821 aattgggcac gtttactcga ttcactattt cttcgatcgg ctctggaata ctgccgaacg
     8881 gatcaaaaga aaaaagaacg atcatgaaga tggctatcag agctatgatt cccgcgatcc
     8941 tcacagatac cgccccacaa tctcctcaat gtttcccgaa gattttatga actccagtcc
     9001 ttcaaggata tccttttcga gatcaggtga attcgtcgaa acgacaaaga catagctgct
     9061 ctttctcaga aactcaggcg ttggagaaag ggagtttctg ctcaagtgct tcagtctcac
     9121 aaaatcgatt tcctctccat tgcacaggaa gatcgaagtt tccaaatagg gaatgccctt
     9181 cgtcctgccg gcaacatcgt cttctgtaag cgctccgcaa atcaaatcta tatcatggtt
     9241 ttctatcgct atcaatgcct cttccttgct ggaaaacttc acaggttcga gatgataacc
     9301 ctctttctca tcgacaagct ggattatctc ccactccaga cccctgaatc cagagggtgt
     9361 ctcttcaatc atcgacggta cctcgacaat gccgagacga acacggcgct gcttcttgag
     9421 cgccattatg taaacgatca gaacaagggc caccccgacc gaaaattgag tcatggcaac
     9481 atccggtgct ccaattatca tcattattat ggctgacagc gaaccgacaa tcccaaacca
     9541 gattacgctt tggaaattgg ttttcgatga aaggactatt atcgcactga caatatataa
     9601 agagctgatt gcgactatga caaagctcat ttctctcttc cccctgagtt tatgaaagct
     9661 cttgcaatga cgtgtgtcac gagaggtccc gtaaccatta gagcaactat gaacgcgagc
     9721 atttcgatta tcgtcaggag accgaagaaa aacgccgaga ttactaggaa tatcagtcca
     9781 accgtatcgg ctacggtcag gaaatgaagt gtaccaatta tcgtgtgtgc aaaaaccgcc
     9841 ctgagagtac cggcaacaac gaagaaaagc ccagggatca gaattatgta ggcgattatt
     9901 tccatcatct gcgcactccc atataataag ctgcgataat cgtcccgctt gtgttgagta
     9961 taaggaacag aagagcaaca ctcgagacga aatcgacgct tgttaccatc tcaatgaaaa
    10021 ttatacctat tagaagtctt gtggagatag cagaataagc cacaagaagc ctccagggat
    10081 tactgcctgc ggcgaaccac acaagcatta caatggagga gatagttgca gccatggtga
    10141 tgtaaaaagt cacttcttca cctcatgaat cagcaatcct tccctgtctt tcgagatgac
    10201 tatttcctca ggggtcatcg ttatagagat gatcttgcca aactccacaa ttctgttttc
    10261 cggagatttc tcactcctga ccgtgaagat gggtctggaa aagataagtc tgaacgcttg
    10321 aaatacggct attggcaagg caagaataat cttgaaagcc gtggaaaaaa cggggccgga
    10381 acgaatgagt ctagatgata tatattgagg aagaacggaa actaacagtc ccgaaactac
    10441 tgaggccaga gtgaatctgc caaaaaaagt gatccacaaa aagaaagaga ttgtgaaaat
    10501 catctagtcc cacctcagag gtttcgccct gtagccttca ttcgactcga agtgctgcat
    10561 ccaaccggtg aggccaaacg catctatcac gttcagtgcg tgatcgtact cttcttccga
    10621 aattcttctc gatagctcat agtattctct cgccctgtaa acaggtcgat actgtgacat
    10681 aagcgatatg ggaacggacg aagataattc aaaagcaaca aactccgcca tttcttcagt
    10741 gcctgctatt ccttcaggta gaacaagatg tctgacgata actccccgca tgttttcttc
    10801 tctgaaagag cccgtttgcc tgaacatctc cttaactgcc ttctttgtga cagtccagta
    10861 atcgggaaca agggagtatt tctttccaac ctcatcgtcg gtgtatctta tgtctgtgag
    10921 gtatatgtcc gcgattccat cgagaagccg cagtgtactc acttcttcat agccgcttgt
    10981 gttaaacact accggaaggg aaaagccctc cttaagagca agttcgtaag ctttgagaaa
    11041 gccatacaga tttggagttg gagtgacgag atcgatgcaa gcggcccctt cctcctgaat
    11101 cttcataaag tgtcgtgata gctcttcaaa agaaaagacc ttgcctgaat tcttctgact
    11161 gaaggcgaag ttttgacagt agagacagct cattgaacag ccactgaaaa agatcgcacc
    11221 tgcaccgcct tccttcacca aaggcggttc ttccccgaag tggagaacag tcgccgagac
    11281 tcttggttcg attttttgca tgcagaaacc agggacctcg aacctgttta tacgacactt
    11341 cttggggcac aggtcacacg agtgcagcct ttcacgagtg ttttctattg ctttttgcac
    11401 tttgacaagt ctttctgcgt ttgaatccat ttaatccatt ccttttcttc actcttgaga
    11461 gttgacgtaa gaccgtcagc tgagattagt cacgcaaatt cgagtctatg gtaactggga
    11521 ttgcgtttgg actatggaag agaaaaggaa cacttcgggc aagttcgcct aaccatcaac
    11581 ccataacgca caaccaatct aacatagtcc tgagagtctt acattccgta atctgaactt
    11641 gtttcagcat tgcgcttcta aaaaccgctg tacgctggtg aaaaacacgt tgtccgtcaa
    11701 cggttcaccg tcctccgaga agatcaaaac cagattccga aacaagttcg ggatgacaga
    11761 gtgaggggct tcctgccgag agccgatgcc ggaataactt tctcagttac agcaaaaccc
    11821 tggattcctg aattgatgag ccggttctct tcacgagacc ccataccctg taatcgatac
    11881 cctgcccttc tcgtcatctg aacttgtttc agcatccagt tccttttcgc cggcgaatac
    11941 cgctcaacga cacatagttc tcttggtctt ccgacctcct accaccaact ttgaacctcg
    12001 tctgctttta ttcatgcaac ttatcctctg cgctttaaag cgttcagcgt atcttaaaaa
    12061 gcggttaacg gctcttctcg gaaaacggtg aaccgctcaa cggagaacaa gttttctttc
    12121 tcttcagacc tcccaaccac caaccctaac ctgttttttt gtttttcttg cttacaactc
    12181 acagcttgca acttggaact gatcctttgc tcttcctagc cccaacctct gaccccgaac
    12241 cacgtttcca ttcattcttc gtcgggatag aaggttatct tcgctcccag tgttctcaat
    12301 ttctcctgaa acctctcata ccctctgaag agatgggtaa cgttgttaat tattgtttct
    12361 ccatcggccg ccagtcctgc tatcacaagt gcagcgccgg ctcttatgtc aggggccacg
    12421 acctctgctc cgctgagttt atcgacgccg catatgttag cgtggctact cgatactttg
    12481 attcttgctc ccattcgatt tagttcatct acgtatccaa atctgttttc aaagacgttc
    12541 tcttctataa cggatgtgcc ttccaccgtt gctagcacgg cagttattat cggctggagg
    12601 tcagtaggaa atccagggta tggttccgac gatattctta ccggcttcag cggtcccttc
    12661 cagaaggcgt gtaaatagtc ttggcctttc tctatacctg ctcccatctc ctcaagaata
    12721 ctcaggagag aaatcaggtg gtccgacctt gttccgtgaa tcttcacgtc gcctttggtg
    12781 gctattgttc caaggagata agtgcccgtt tcaattctgt cgggaataac cgagtattcc
    12841 gatccatgga gatttctaac tccggtaatc tttatggtag gtgtcgaggc accggcaatc
    12901 tgtgcgccac acgcattaag gaagttctgg agatccgata tttcaggctc gcgggctgca
    12961 ttctcaatcg ttatcttgct tccctccatt agagccgccg tggtcatgag ctgctcggtt
    13021 gcaccaacac tgggaaaggg gagcgcatag acaatctctt tttcaaaact gttgggaatg
    13081 acagccatga cgtctccatg ttcctccgtt attctgaaac caaaggcctc gagacccttc
    13141 agatgaaagt cgacgggacg ctgaccgatg ttgcagccac cgggttttcc tacctttgcc
    13201 cacccgcaaa tcattgcaag agggccaatt atgttgaatg aggccctcat tttctttaca
    13261 agctcatacg ggacgtttcc cactagtatt tcccctggca ttaccgttac cgtctcattt
    13321 tcgaaagaga ctgtctttcc aatctgctga agtatggaaa tcattgtttg aacatcgcgc
    13381 agttcaggga cattttttat cactaccggt tcttcgatca tgacagtcgc tgccagaata
    13441 ggcaaaactg aattcttgga gccgcttgcc tttatctcgc ctttgagact tgtgtttccc
    13501 ataacaacga gctggctcat aataaccctc caatcatttt atcgtgtaaa aaagacttga
    13561 ccgcactatt attttatcag aggaagaggt tgcaagaatg tccatagaat tcagagactt
    13621 tattgactca ttgccttttt gtacatgcga agccgtattc taagctctgc aacttcacct
    13681 gtacgatcgg gtttcaactc tcccgcgctt gacagatcag gttcgctctg gctgaacagt
    13741 aatccttcct ccgatattga catctcttga ggaagaaatg gtgagaccgg ttacaagtct
    13801 tcttctgaga atcctcgcaa agtatcttct tatgaacagt ttgcagtctt ttttgaagat
    13861 atccaggtcg gacttctccc aaatacttcc caacgatctt ttctgctctc aagttcatcg
    13921 ctggctgaaa ccaaaatgat tgggcttagt cccagaagtt tttcgatcac cattctggtg
    13981 aagatggaag ttgctttgct gccatcattg ctattggtaa ggacagccga aaggtttaga
    14041 tcgagaagta ctgccagcaa ggaaatctga ccgttgaccc ggcacccatg ctgcaggact
    14101 ttgacgcctt caatctcctt gacgaaccat cctagagcaa ctctggaggc cggagaagca
    14161 tcgattcttc ccgtggtcaa ggcctcaaat gagtcttgac ttatgattcc agtcttatgg
    14221 gggacgattc agcaaagtaa aatgcagaat acttaagcaa attctcggcg ttggtaacga
    14281 ttccacctgc cggatgcata gctctcttta tatgccaagg ttgtcaacgg atatcttctc
    14341 cttctcgaca tgatggccgg cggcaaaccg ttcggtaatg atctattcgg gagagaaacg
    14401 gatcttatcg agatcgagag gttttgaaac caattcattg atggcttctt cgaagggctt
    14461 tccggtaatc gcttcgatga ctctgcttgc gacatgaaac cggaattgca gtacgaaata
    14521 atcatttccg gggggattga tctgtggaag tgtcaatgtg atcttaacca tgtttcccat
    14581 aacatttttg ccaaaacgga aatccttgaa gtaatctcct tttcccgata attgaataag
    14641 aagatgtttc attgtcaatc tcttctcggt ctctccgtcc gagagcttga agttcggtaa
    14701 gtaatcaagt aatggtcttt caagatcaac tctgccgcgg tctgccaatc tcattactgc
    14761 cagagcgacg aacgtctttg ttatagagct aatctggaag aaattgtcct cattcacttt
    14821 cagtggattg ctgaggttcg taacgccata agaggcagtg tcgacagttt ctccgaagca
    14881 aattctacat gacaggcccg gtatctttct tatgcaacaa cctcttcctg tttggtcgcc
    14941 atattgacaa agccgtttct caaagattca tgaacacctt ccttaggcat tatatccgaa
    15001 gtgtgtttaa gggagactaa tctctggaat taccgtttag tctaatatag aattgtagtg
    15061 aggtgacgaa aatggcaaag ttgaatctgt cggacctgct aataccgatg agtaaaaggg
    15121 ttaagttcga agaaaaaata gggctcgttc tttcgggggg aggagcgcgt ggcgcttatc
    15181 agatcggagt atggaaggcc ttgaaagact gtgggatcga aattggggga gtttacggaa
    15241 cctccgttgg cgcgataaat tccatggcag tagccatgga caatttcgaa gatgcaaggc
    15301 aactgtggct gaaaatcgat tttgacaaag ttgtgaaaaa cggccccgac ggaaacctaa
    15361 tcgaaaaagc cctcgctgcc ttgaagagcc gtggtttcga tcctgcacct ctgagagaga
    15421 actttggtcg acttcttaaa gaagaattag tacgaaaggc gaaaatcgat gttggaatcg
    15481 tcacctattc tcttagcaat atggaaccca aggagctatt cctagacgat attccagaag
    15541 gaaagttaga agattatata cttgccagcg caaatcatcc ggtcttcaag agagaagaga
    15601 tcggttcaga gaaattcatc gatggaggaa tatacaggaa tatccctgta aacatggctc
    15661 ttagcagagg cttcaaagag attatcattg tggatctcgg tcccaagaga ataagggatg
    15721 tgcttaccct cacttcgctt aagagattgg aggaagtaaa gtatctggtt atcaagcccc
    15781 gcgagttcta tggagacgtt ctcgatttcg accctgaaat agctagaaac ttcatgagag
    15841 aaggatatct taattgtcta gatgacttgg gatatttgaa cggcgaggag tacttcgtct
    15901 ttgccagtca cgatacaatt gcacgggctc tgttttctct ttctaagaag aagaaagagg
    15961 aggccagggc gatctttgca gttgaaccgt ggcagagcga atctactcac catttctatt
    16021 acggccagct catgccggtt cttcaggatc actttggtat cttcaatcct gttgaaacat
    16081 gggtacgact gctagaagac cttgcacgtt atcttgaact ggagaggctt gatctttatt
    16141 ctctctacag cttaacaaag gagattctct caagtcgtaa cgttggagtt gaaggaagaa
    16201 actacaacga tgtttcctgt gaaagcatac tgaacttcct tgaatttctc gtgaaaaact
    16261 cggatctaga aagtcttgaa gaccgtgaat tcagtctgtt tcgggaaggg ttcttgtcat
    16321 tgaccgatct caatgactag atgaaaaggg tccggcagaa ccggacctca ctagcttaag
    16381 gatttagatc tagtagttct caacgaaaag ttcgaagtaa gcctttgggt gtttacaagc
    16441 cggacactgc tccggagctt ccttagcttc gagaatgtag ccgcaatttc cgcacttcca
    16501 aaggactttt tcttctcttt tgaatacctt accggtttcg acgttttcca gcagttttag
    16561 ataacgggcc tcgtgtctgc tctctacttt tgctatttgt ctgaagctat tagcgacatc
    16621 caagaagcct tccttttctg cgacatcagc gaaggtcggg tagagtttgg tccattcctc
    16681 gttttctcca gcagcggccg cctttaggtt ttctcttgtt cctcgtagat ccactggata
    16741 gtcggcatcg acatgtacca taacgggtaa ttcatctttc ataccgtcaa taatgtgttt
    16801 gaaaaagact tccgcatgtt ccttctcgtt gtcggcggtt tccttgaaga ttgccgatat
    16861 ctgttcaaag ccttctttct tcgccacaga tgcatagaaa gtgtacctgt ttctagcctg
    16921 tgattcaccg gcaaatgcct tcataaggtt ttcaacagtc tctgagcctt tgagattcac
    16981 aatttcaccc ccgaatattg gatgaggtac cgagtattga tagttgattg atgacctgac
    17041 gaagagcttt ttcaaaacaa ttatagtaca gatttgatta caatcacgaa tttctctaaa
    17101 aatgatgagt taaaggtttc tgcctatatc tggctattcc gatttcttgt ctttgttatt
    17161 caggctttca ttaaattcct tagaggagtc gatcccgaag ttctcgtctt cgacattgcc
    17221 agagggttct acatggacat ggacatcgta gacatttttg tgatcttctt ttatagaggc
    17281 ctcgactttt tttgcaagag aatgtgcctt ctttaccgac atatccgggt ctacttcaat
    17341 atctagatct acgaggtact tataacccat ttttctcact ctaactttat gaggattttg
    17401 aacaccctga accctttcta tcgaatcgaa aactaccctg taaatgtcgt ctccaggttt
    17461 gagcccgtcc atgagttcgt aattggtctc ccaaaagacc tctatggagg tcttgattac
    17521 cagagcagat acagccaacc ctacgatcga atccactatc tgcaggccag taattcccac
    17581 gaagatcgag cctcccagaa cagatagcga gatgacgatg tccagtttca tgttcgttgc
    17641 atcagctatg aagaccgatg aatcaatctt ctttcctatt cccagcttat aacgatatag
    17701 aaaatacttc gcagcagagg agatggctgc cactaaaatt accagagcga tattctcaac
    17761 agaggatccg ccgctgaaga gcttctgaat ggaactaacg gccagttgtg ctccggcaaa
    17821 gaaaataacc atggatacga tttttgaggc aacggcgtct gccctttcgt gaccgtacgg
    17881 gtgctgcgca tcaggcggct tgtttgatac ggatgtagag atccaggtga taaccgatgt
    17941 caaaatatcc gtagtcgaat ctactccgtc ggccaaaata gccatacttc cggtgaagaa
    18001 accggtaaga accttcagca ccgcgagcgc tgcatttccg gtcaccccta ttattgagcc
    18061 tcttcttgaa agcgccactc tgtcctcggt catttagtca cctcgattgc gctcgagaaa
    18121 cctccaaggt atctcttgcc acgataccta cttaaagagc cgttgatgat ctgaagacta
    18181 gaaacgtctt ctaaagcgac acgctttttc ccgagattgt gataaatgaa cgcgttccgg
    18241 ctttcaccaa cgactcttaa gattaccagg ttttctcttc gtctcaaacc tcttatagtt
    18301 gccaacctca gccaggagtt gttcttcctg aattcaagta tagccctgat tctctcgaag
    18361 tagcttccat ctacagcttt ttgtgcttcc agagacctac cgctgtgagg atgattcttc
    18421 ccgagcgctt tccattcggt ttgaccggga ccgtagccat tctcgaacca caggaagggc
    18481 tcaagttgct cttcgttgaa atacgcatta tagacccctt ccattccaag ttcttcgcca
    18541 taatagaaga agggcactcc gggaagagac aacaatactg acattgcaag cagagagagc
    18601 tctttgtcac catccatggc cgaaactaga cgactcatgt catgatttgt taggaaaatt
    18661 ccagactcat agctacgctt cgatctgaaa tatctattga gatcagtcct caaagacttc
    18721 actaggagtt ctgggtctct tgtcgcaacg gaagttttca agtcctcggc aaggggaaaa
    18781 ttgaatccaa ttccaaagat agtggagtac aggtctacta tttgcgcatc gtcccatact
    18841 tcactcacaa atatcgaatt tggagaaatc gatctgcagt gtgccgtcat ctccttccag
    18901 aaagcgatgt ttctgctgtg ctggtattcg cagatgcctt tttcaagaga aaaatcgaaa
    18961 atgtgctttg cagcatcgaa tcggaatcca tcgaatccta cggataacca gaatgtaagg
    19021 acctccttca tctgctgcca cagggaagaa gactcataat tgagatcggg acttccaggt
    19081 ccgaacaatg tgtagaccca ctgtcccgag tagtttgtcc atacctgctc tccatcccaa
    19141 tgtcttctag ccttcagcca ctgttcgtct ttcagaaaaa gataccagtc tctgtatggt
    19201 ttttctccat ccaaggcctt caagaaccat tcatgatttg gagaggtgtg attcatcgga
    19261 agatccagta tgactttcaa atctagtttg tgtgctgatg ccagaaagtt tcttaaatcc
    19321 ttcagggttc catagattgg gttgacactg aagaaatcga tgacactgta gccatggtaa
    19381 gatggcgaat gaagaatcgg caaaagccag attccttcaa tacctaaatc cgcaagatag
    19441 tcgagcttcc gctcggctcc gataaaatca ccaattccat cgccattgcc gtcacagaat
    19501 gatctcagat agagttcgta aaacactcga atccctcccg aattagtcgg ccgagtaatt
    19561 ggactgactt gcgagaacca ttgtttccct ttgaatcata gatcttacca tagttatagg
    19621 acagtgcatt gtgttaaaat cttctctgtt ccgggaggtg tggctagatg ctgataaagg
    19681 aaattctgag taaaggtgaa cagatgaatg caagcgacat acacttaacc tcaggtgaaa
    19741 acgtcatata cagggtcgac ggtgagttga ttcaagacaa actgttgacc gacgaaataa
    19801 tgatgaagaa gataacgaca gaacttcaag agatatctga gattaagtta caggattcgg
    19861 aaagtaagga gattgacttt tcttttgggt tcagctcttt aaggataaga ggaaactact
    19921 tctattcaaa taagcttccc gtagtcgctc tgaggttaat cccgaaaaca atcaagactc
    19981 ttgaagatct gggttatccg ccgttgttca aggaattctg taaaccagat aagggcctcg
    20041 tacttgttgc aggaccgacc ggaagtggaa aatcgactac tcttgccgca atgcttgaaa
    20101 acatcaacag aacgagacat gttcatttga tcaccataga agatccggta gaatacgtat
    20161 tcgaaccaaa aaatgccctg atccatcaga gggaactcgg atcggatacg aaatcgttct
    20221 ataatggact gaaatatgca ttgaggcagg accccgatgt cattctagtt ggagagatga
    20281 gagatagcga aactatggaa cttgctatga ctgctgctga gacaggccat cttgtattct
    20341 cgacaatcca cacaaattcc gccgccacta cacctgaaag aatcgtagga gtctttccgc
    20401 ctcaccagca gaaccaggta gctatgcagc ttgccaacac tctgctggcc gttatatacc
    20461 aaagattgct caggagaagg gacggcaaag gaagaatcgc ggttctcgag ataatggtgg
    20521 tgaaccaggc tataagaaat cttataaggg agcagaaatt ccaccaaata gaatcgatga
    20581 tgcaggccgg gataaagtat ggaatggtca cctttgatga tgcgttgatg gatgttttca
    20641 aaaagggaat aatcgacaag gaccagcttc aggattacgc aagggatccc aatgcaatgc
    20701 tcaggagggc cttctaatgg atttaggcaa gataatggaa gtcttcaggg agagattcaa
    20761 ggaggtcgag aaggaactct caaaacccga agttgcttca gacccggaca agctcatgga
    20821 atacgggaag aaacatgcag agctctctga gatcattaat ctttacagcg aaatagactc
    20881 gatgaagggc gaacttgagg aatggcatga aatgaaggct accgcagaag agaacgaaca
    20941 ggaagtgatc gcgaatacaa tatccgatct cgagaacaga atcgcaagaa aggagcttaa
    21001 tctaaaggga cttctcgtcc ccgatctagc agatgagagg aatattatta ttgagataag
    21061 ggccggtaca ggtggagaag aggctgcatt gttcgctgcc gatctcttcc gaatgtacac
    21121 cagatatgcg gaacgaaaca actggaaatc agagataatc gattcaaatg agacggatat
    21181 cggtggattc aaggaagtca tctttcagat aagaggcaga agcgtattca gtaaactgaa
    21241 gtatgaaagc ggagttcaca gagtccagag agtgccgacg actgaatcgg ggggaagaat
    21301 ccacacgtct actgcgacgg tagcggtgct tccagaagta agcgaaacag agatcaaaat
    21361 cgaccctgcc gatctcagaa tcgatacgta tcgctcttca ggcgccggtg gccagcacgt
    21421 aaacaagacc gattccgccg taaggatagt ccatgagcct acagggatag tagttgctgt
    21481 gcagaaagag agatctcagc atcagaataa agctagagcg ctcgagattc tacgtgcaag
    21541 gctgtatgaa atgtacagtt ctaaagcaga gaatgagatt actgaggcaa gaaggacgca
    21601 gatcggtacc ggtgaacgaa gcgaaaagat aagaacttac aacttccctc aaaacagggt
    21661 aactgatcac aggataaatt ttacaagcta cagacttctt tatgtgatgg acggagatct
    21721 cgacgagcta gtgacggaac tttccgctgc agatataaac aagcggctgg aattacttca
    21781 caagaaactg atcggttaaa acgacttcag ttccaatgga gaatcgaatg aaaagcagaa
    21841 agcctttaaa gacaaatgcc actagaatcc tggacgggct tgggattgaa tacgagattc
    21901 tagaatacga agtcgacgaa cacgatctga gtgccgagaa cgttgcatcg aagctgggct
    21961 tgtcaccttc gcgaaccgtg aaaactattg tcttgaaggg tgacagaagc ggggtttttg
    22021 tctgttgtct cggcggtcat agagagatcg atatgaagaa gcttccagag atcacgggtg
    22081 acgggaatat cgagacggtt ccttcagaaa gtctcctgac gcttacagga tatgtcaggg
    22141 gaggagtttc gcctctcggg atgaagaaga gcttcaagat tctcgttgat aaaggagttt
    22201 tgaatgaaga aaggatatcc gtgagtgctg gcaaacgagg tcttcagatc tggttgagac
    22261 cgactgacct tgtctgcgca actaatgcca gtgttgtctc cttttccaaa gaaaagcatc
    22321 agacgttgta atggtatccg aacaatgctt cttcaaatct tgaaaagtat tacaaggacc
    22381 gaaaccaaac caaagaaaag cgccaacaac aacaaaacag tagtaatttc caaatcattt
    22441 ctctcccttc ggatttctgt caatcaattc tacaatttcg caggtgctca cagaaggggt
    22501 taagattgat ctgttttgca agtactgaaa ggcattgtaa aattgaatac aaaggacggt
    22561 gataaaatgt atatccgctc tggtgatccg gtgagaacaa taataaaaat tgtcggtgga
    22621 atagtgtatg ttgttggaat aggtttgata atattcagtt ttgccagttt ctttcttgct
    22681 ccttacttga gtctggtctt cggtggcgtg attgtgtttc tggtattggg tttcattgcg
    22741 acgacactgg ggagaatgct tcttgcaatg gcgaagacaa agagcttctc cggccccgta
    22801 agtgaacagg aaacggttag cggtcgagaa cttaaggaag atatgaccta tgactacaca
    22861 tttgtctcag acagagaagc gagaattgag aagaccaaga gcaagagtta ttccagatgt
    22921 cccgaatgcg gtgcggagaa tgaggacaat gcaaccaaat gctcgaactg cggcgcttct
    22981 cttgtaggtt acaagaaatg cagtctttgc tacatgctga ataagaaaga gaatcgcttc
    23041 tgcaagaact gcggacacga tttcacgctt gactgagtta agagagcaag tcacactgtc
    23101 gggcataaga gccgtcattt gagaaaacgg ttcttcgttc tgacgctacg cgtccaggtt
    23161 cttggttctt cgtcagggat tccgtccacc gagaggatca aaaccagatc ccgaaacaag
    23221 ttcgggatga cagtgtatga agatttcgaa acgtcgcatc ccgtcattct gaactccatt
    23281 cagaatctct cgttttttcg ctttgaccaa gaacgaagat gttttatttg gaggacggtt
    23341 aaccgtccaa cggagaaccg gtttcacttc gctctttccg accactaacc tcctaccacc
    23401 aaccacggtt cctgaaaaaa cccagatcct gaacaggagc gcttcag
//
LOCUS       DAOQYI010000027        23140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_2561_length_23140_cov_82.255750, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000027 DAOQYI010000000
VERSION     DAOQYI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 23140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23140
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2561_length_23140_cov_82.255750"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKL93_07725"
     CDS             complement(<1..566)
                     /locus_tag="PKL93_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNQ71183.1"
                     /translation="MSGFRPEVWVPSKELVSRESLSRMIEKLDGINAVRAYIQVVNRA
                     DSYYSSNVLPPAEELVQDFDALQMTIEKACEREFRISAWMNVNLVWGFGKRRPFSESH
                     ILNRRPEWITADENGTSMLEYPSSNLEEVFGPYIDPAIPDVREFTARIASEISNYDVE
                     EVHLDFIRYPFKSFGYNPLALAEYRKWL"
     gene            complement(608..1459)
                     /locus_tag="PKL93_07730"
     CDS             complement(608..1459)
                     /locus_tag="PKL93_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HNQ71184.1"
                     /translation="MRTNKASVVKISVSGEVAHPLRRSPFRLEVDGTPRLFPGTGGIT
                     YNFALGDSAFKMVGDHVEPDVSTKNPDNDKNAAYVGYSCIGNSATLISGDAKGEKGIV
                     IGKHGGINHVLIHFKEDIKKKMAIGDKIQVVGFGQGLVLEDYPSIRVYNIDPELLDRI
                     PVGEGDGKIHFPVRAIVPGYLMGSGSGSGDPSGGDYDIITNDRALIKKVGLDRLRIGD
                     FVALENHNDSFGLGGYMEGSVTIGVVVHGDCIITGHGPGVTVVMADGKGIIIPEISEK
                     SNVIDFI"
     gene            1568..2404
                     /gene="ylqF"
                     /locus_tag="PKL93_07735"
     CDS             1568..2404
                     /gene="ylqF"
                     /locus_tag="PKL93_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNQ71185.1"
                     /translation="MFYPGHIQKAKRQIQNYIKSVDGIVELLDARIPLSSRAYEAEKL
                     FQKKQRIVVLNKSDLADPKITSMWRDYFKSEGSDVVEASLRSTDAKQFIIREIVPLLK
                     SRFYEKRFMVVGMPNVGKSTFINRLKGKKSLAVGNRPGITRGVQWINVSESIAVLDTP
                     GILYSDLRSPHITTKLLAVGSLPYEKFDPLDAFERVLALIVERYGKGLLEDYLGEEFS
                     NQEEFVEKFCRRRNYLAKQGALDTTRGAHTFLREVAAGKAGRLSFEDPESFYTSDSPE
                     AE"
     gene            complement(2374..3609)
                     /locus_tag="PKL93_07740"
     CDS             complement(2374..3609)
                     /locus_tag="PKL93_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ71186.1"
                     /translation="MGLAERVMELGKKLFRIQNVDGSGPMAFDSSPGLYIHVPFCPEF
                     CSFCPYNKIKYDSKLTNKYLTALKRESELQRVGQFSSIYIGGGTPSYDLDLLKGVVSH
                     FREMTDGEIALEIHPADVSSKRLEEIRETGVNFVSLGIQSFDDFTLNSMGRRRQDSVV
                     SLNSIAATTSAGFDFVDVDLIFDYQLERRRIVEDLETAISYGPEQVSVYPMMRFADTA
                     YKNTKNDPEKELEILEELENVALSKGYIRDSLWTFKKRNEGRKYSSVSREFFLGIGTS
                     ASSFNGKEFRTNTFSLDEYYSFLDSGKLPVSKTIKMVKFSAILYYSFWALYGGQLDLR
                     RLEEHFGRLPVRMRYLLHSAIARGYFQREEGIVKPTRIGTRKLHIVEEWLTYSFIDRI
                     WSDLRNKSRNHSASGESLV"
     gene            3700..5046
                     /locus_tag="PKL93_07745"
     CDS             3700..5046
                     /locus_tag="PKL93_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ71187.1"
                     /translation="MKKDVVIVGGGPAGIVTAMTAKKTYPDKSIVVIRREREGVVPCG
                     IPYIFHTLPTVDANTMPIKGAEDLGIDFIFDEVDEISTDSKMVKLSGGESIEYEKLVI
                     ATGSQPIFPPIKGSKLPGVFTIAKDREYLKSVYAAAKSAKKVVVVGGGFIGVEVSDEI
                     LSDGKEVYLVEAVDQILMAAFDKEFGTMVSERLEKKGMKLRTGMKLCEIKGEDKVSGV
                     VLDNGEEIEADMVILSIGYRPNVKLLENIPVHRGITGGIWADEYMRTSVQDVFAAGDC
                     VEHKCFFTRKPSRLMLASTAAFEARVAGSNLFSLKMVRENHGNLGVFSTSVAGLTVAA
                     AGLTECTAKMENFDYVVGVAKGIDRHPGSLPDKSEIFVKMVFSKESGILLGAQMAGGK
                     SVGEMINIIGLGLQKGITVNDFLTMQIGSHPLLTAAPTSYPLTLAAENALMNLRKE"
     gene            complement(5106..5852)
                     /gene="gpmA"
                     /locus_tag="PKL93_07750"
     CDS             complement(5106..5852)
                     /gene="gpmA"
                     /locus_tag="PKL93_07750"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNQ71188.1"
                     /translation="MTKLVLVRHGESTWNKENRFTGWTDVDLSEKGREEAENAGKVLK
                     ADGYDFDLAYTSVLKRAIRTLWYIMDEMDLMWIPVIKDWRLNERHYGALQGLNKAETA
                     AKHGEEQVKIWRRSYDIRPPALEESDERFPGHDPKYRSLSDEELPRTECLKDTVARFL
                     PLWKNEISTQIKSGKKVLIVAHGNSLRALVKYLDNIPDEEIVGLNIPTGIPLVYELDD
                     GLKPIKHYYLGDPEEIAKAQQAVANQGKAK"
     gene            complement(5951..6772)
                     /locus_tag="PKL93_07755"
     CDS             complement(5951..6772)
                     /locus_tag="PKL93_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNQ71189.1"
                     /translation="MSEAEAVKLSNNGPVTTKSLISSLKQLGVKRGMVLLVHSSLSRL
                     GWVCGGAVAVVKALETILDEDGTLVMPAHSGDLSEPSNWTNPPVPESWFDPIRRTMPP
                     FNRVETPTRGMGVIPETFRKMKGVIRSDHPQVSFVARGKRASYIVDNHELEFGMGEGS
                     PLARLYDLNAYILLLGVSHRNNSSLHLAEYRADFPSRKLCKDGAPIIENGKRVWREFI
                     DYELETEDFETIGKTFEEAFPTTVNRGVVGYGKAVLIPQVELVDFAVEWMTKNRK"
     gene            complement(6808..7203)
                     /locus_tag="PKL93_07760"
     CDS             complement(6808..7203)
                     /locus_tag="PKL93_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71190.1"
                     /translation="MTLTLIDVGRFFTILGIIGLVILTLCLAGFDGYESKTLYARDSG
                     NSFATVSLRPGQQLIARMDKKVMIGNNAYLVVERDGKEVASLADDWEETKNELIFEGD
                     GEYNLKVIFEDSSADRRSAEIEIKWLIAK"
     gene            7481..8107
                     /locus_tag="PKL93_07765"
     CDS             7481..8107
                     /locus_tag="PKL93_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNQ71191.1"
                     /translation="MKIIIFGGTGRVGRVILDRALGENHIVTVFVGDVSKLTVRKENL
                     RIFQGDVFNSQSVRDAIRGQDAIISALGPDNSGSVNDTLAVAMKNIVSGARETEVNKI
                     VTIANSGILQLSPRELRLDSPNYPQYLKKSSREFLDAFEILKTSELDWVVVCPPFMTF
                     SEGNQSYRVSADFLPENGVKISVQDVAEFAFKQLFTSEYSQKRVGIAY"
     gene            8120..8731
                     /locus_tag="PKL93_07770"
     CDS             8120..8731
                     /locus_tag="PKL93_07770"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNQ71192.1"
                     /translation="MKRESPGSEIRINRLEALTDGVFAIAMTILVLSLEVPSRSQVTS
                     QSELVQNIMSKGYQFLSYFISFFVIGSIWISTIRRTHILNKTDYKHLWLNMLNLFFVT
                     TIPFTTSLLGDYGEYEFAEVLFHLDILILETIALVQWQYLMNNKDLIREDAIDDSNLI
                     YYRKVYIFHVIVPLLGIAVSVFTPVWSNLVYLVIFFRVFFIRR"
     gene            8782..9138
                     /locus_tag="PKL93_07775"
     CDS             8782..9138
                     /locus_tag="PKL93_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HNQ71193.1"
                     /translation="MEIAFTIIRFVVAVLTAVTGVYIVLRPESYARLAEFSTGNSRGR
                     TEVKTIMGGTFIGLGMAPIILLGSTIAFIFLGTVYLFIAATRILSMFVDKSFVFTNLI
                     TLLVEIAFGLIMVIPV"
     gene            9225..9941
                     /locus_tag="PKL93_07780"
     CDS             9225..9941
                     /locus_tag="PKL93_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNQ71194.1"
                     /translation="MREEDLKRAILEAWSKRLGFSSDRFFLGTYFFFPDESKRGKGGI
                     EQLSFERQTVILYDPLLEDVFKSIETQDENPSDYLADLIRDSFLLLKKTSIKYLNPSN
                     YVPFYPPAEFTVRRLNQGDRTAFYRFRNQCTREDLAEGFVELDHVAVFGALYEDEIVA
                     VSSIIVWDEIADIGIITMPDFRELGLGKALGSRASEWAVINKKLIQYRHNVLNYGSLK
                     VSRALGFREYIVDQEYSYKG"
     gene            10303..>10583
                     /locus_tag="PKL93_07785"
     CDS             10303..>10583
                     /locus_tag="PKL93_07785"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488399.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNQ71195.1"
                     /translation="MVVVLKRGTGEEEIKQVESLSESLNLSCHVSRGLERVVIGVIGD
                     DRYMSVERFEALECVEQVVRVLKPFKLVSREFHSEDISVEIGDLSVGEG"
     assembly_gap    10584..10683
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10684..11780
                     /gene="aroA"
                     /locus_tag="PKL93_07790"
     CDS             <10684..11780
                     /gene="aroA"
                     /locus_tag="PKL93_07790"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNQ71196.1"
                     /translation="ELGGNFKGGFDRMEISSASWKESLRPLDCGNSGTTARLMAGVLS
                     GKEGFHVLFGDDSLSGRPMERVVSPLKEMGASISARRDSLLPMCITGGKLRGYEHSLS
                     VASAQVKSAILLAGIQAEGTTAVTEPHCSRDHTERLLRSMGAGITVSDKRVEVEKSEL
                     LPVRFSIPGDVSSAAFPIALAVLHENGKVTIRNVGLNQGRTGFLRLLMKMGADIEIEV
                     EESLPEPMGTIVTRSSRLIGVEVGSDLIPSMIDELPLIALAGVFAEGTTTVRGAGELR
                     KKESDRISVTVQNFRKIGVEILEHEDGFSVEGPQRITGGKVDSYGDHRIAMLFSIAGL
                     LSSEGVEVTNSNAVGVSFPEFFKTLEEVCQ"
     gene            11777..12559
                     /locus_tag="PKL93_07795"
     CDS             11777..12559
                     /locus_tag="PKL93_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNQ71197.1"
                     /translation="MKLCIIGHPVSHSLSPAIYGWFFERMGIDATYEAVDILPDEFSE
                     EITSVAEHFDGFNVTIPFKEKIIAHVVSQVEPPLSAINCVFERRGYNTDWVGFGKPLL
                     ERSIEEPVMIIGAGGAARAVIFFLRKAGVKRIELLNRTLSRAEKIKKEFEIPGLFEVS
                     VFPFESIREVASRSKSIINASSLGMNGEETGITSEELVGKSLVYDLIYWKTPLIELSH
                     DCGVETVLDGRDMLLHQALENLKIWGLPSGDVFEKTFWEVVR"
     gene            12556..13683
                     /gene="aroC"
                     /locus_tag="PKL93_07800"
     CDS             12556..13683
                     /gene="aroC"
                     /locus_tag="PKL93_07800"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNQ71198.1"
                     /translation="MKFTVAGDSHGSGVFGVIEELPSGVSLSIEKIDRQLQRRQKGYG
                     RGQRMKGEEDRAIVKSGLWKDLTTGAPLLIEIENKVSSAEKSVRSIPRPGHSDYAAWT
                     RYKLNDLAVYAERSSARWTAALTAIGSVASQILQELGITLCSSVIAIGKVSCERPTGT
                     EWISRNCGSPVFCHDEIAYREMLKEIDKANELGETLGGRFVVIADGIPAGTGGYGSLF
                     ERLDSRIGKHFMAIPSVKGIFIGNPDVSLRGGEYHDKLYIKDGIVFRQTNNAGGIEGG
                     ISNGERIVVEASVKPIPSLRRGISSVDLSDLNETKTPYVRSDTTAVPAAAIVGESMLA
                     LLLLEAILERFGNGDFCSIKRRVKDESIPHWNDGFGEEHDR"
     gene            13631..15118
                     /gene="aroB"
                     /locus_tag="PKL93_07805"
     CDS             13631..15118
                     /gene="aroB"
                     /locus_tag="PKL93_07805"
                     /EC_number="2.7.1.71"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase
                     AroK/3-dehydroquinate synthase AroB"
                     /protein_id="HNQ71199.1"
                     /translation="MRVFLIGMMGSGKSTIGRILSSVLDKKFIDMDSEIERAKGMSIS
                     EIFEKEGEKEFRRLEKNLLKELVRSDEIVVSTGGGVILDNENRQTLKGENSIYLRLPP
                     ADLYRRVSVKGRPLLREGKESIFRIWEERRELYEQFPSVDTSSQTQWETVAAISMKIA
                     AGERKTLNSSSHPVSISPGGFKSIGRMPNTVVSKRVQRIFSEWIPSSAFSIDDGEEAK
                     GFHTLFQIYEYLMDRGVSRSDMVVGAGGGTVTDLVGFASSTFKRGVPITLYPTTLLAQ
                     VDASIGGKNGLNFEGCKNVVGNFYMPYETIIDPVVTLSMDEGRFEEGLVEAFKIFLIT
                     GRWYENFKSRCRDLKNRNLRALSEMLEEAVRQKDRIVAIDTRETGMRRILNLGHTLGH
                     LYEPLAGVSHGMAVAWGLEREMHYFANLGLIDEEMYREVSETLSEIVGLDFPQLPVED
                     ALRLLRNDKKATTSESMEIEIPLVRKPGSFEFAKVRLDDLLAVVV"
     gene            15115..15567
                     /gene="aroQ"
                     /locus_tag="PKL93_07810"
     CDS             15115..15567
                     /gene="aroQ"
                     /locus_tag="PKL93_07810"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNQ71200.1"
                     /translation="MKILILNGPNLNMLGMREKSLYGSFTYSELSEAIERKCLESGAS
                     SELFQSNHEGDLIERIHNIDYDAIVINAGALTHYSYSLRDALELFRGIKIEVHISNIF
                     AREEFRSKSVISPVCSGTIAGLGLNGYLLAIEYAVSHLKSLTDREATE"
     gene            15564..15923
                     /gene="aroH"
                     /locus_tag="PKL93_07815"
     CDS             15564..15923
                     /gene="aroH"
                     /locus_tag="PKL93_07815"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNQ71201.1"
                     /translation="MKAIRGATSIEADCPEEIRNSVIELMEEIFSRNKITELHSVIFT
                     VTNDITSYNPATAFRKVFNQSDVALLCLYEASFENSPGGIIRVLLHCESETKNFVYLR
                     RAKNLRPDKVQAGDSKA"
     gene            15920..16687
                     /locus_tag="PKL93_07820"
     CDS             15920..16687
                     /locus_tag="PKL93_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNQ71202.1"
                     /translation="MKVHIIGAGSIGGSIALRLSKRGHRVSIFDQNVVTTEMLRKKDP
                     SIEVSSDAFTPGDLTVIALPMNSEEELLLSADFTGPAFDVASVMQPFQEIARLRKIRL
                     ISGHPMAGNVHKGPAGWDESMFDGRVFLFSPGEFATGEDLGLVLMVVSELGSSPEYLP
                     PERHDYIVGRISQTAYFLSRTLLRLGGDFEKYSGPGYASTSRLGRQNMDMVLDMARFN
                     GRNIASSLEEAESYLHDIRTAIEEGDLSKLEELISGY"
     gene            complement(16795..17238)
                     /locus_tag="PKL93_07825"
     CDS             complement(16795..17238)
                     /locus_tag="PKL93_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71203.1"
                     /translation="MRALCALMEREMAPKKIGWYEGFNGRDSLFGFVSYAEEIPVAFF
                     EVMPSLLSPYSIPKSLSTALISCIYNSHDSTYDYRSELIQLSVEECRKRGFGSVEALS
                     GKKSSFSNGLASFFLQNGFEDLETVDSSVIMFSGRDDIDLLIRNL"
     gene            complement(17575..18195)
                     /locus_tag="PKL93_07830"
     CDS             complement(17575..18195)
                     /locus_tag="PKL93_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71204.1"
                     /translation="MQRREWLERYRKDVLEQTCTGLEIDKSQLQDFILRMTGFLEESN
                     IIADFGCHAQDFWPLLREMKLRVVGVSCSKAIKQTQGIRVMNIGFSDFNFLGIFEGFV
                     ASGILQNLHPELWSKTLLRIARSVKTSGVGLLVTGIGNEKESKKRCETLKKSGLPVVI
                     GEYIDDDGNYNFRPLKSWYDQWLKNAGFYVFREEMIRDTVYSLIKK"
     gene            complement(18268..19062)
                     /locus_tag="PKL93_07835"
     CDS             complement(18268..19062)
                     /locus_tag="PKL93_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71205.1"
                     /translation="MSKGIAWLLVVLISAAAIVNLATIVTASNKLLGEISTVRTDLFT
                     LKSRVASLDTVVADIRNRIEQSGDFVKSVHFTRSSTTLEKVSLKASVTLSEFTEGSQL
                     FLNIIDEDAEVRSYELKSENGVFTAMLELLPDETYRGQVRVIDDDKETLSNEFAIPQD
                     LYNVQRYQLIGHAWLLETDRIHYSFDLYTHYCRDEELRISEAAIKIVEGEDTRETLFL
                     PLDNSQELAKTLYYEADRDSSLTFVAEISYRFGESKTEEVKINYHF"
     gene            19179..19832
                     /locus_tag="PKL93_07840"
     CDS             19179..19832
                     /locus_tag="PKL93_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNQ71206.1"
                     /translation="MIAENVKRLRSEIPDYVTIVAASKTRTASEILEILESGLKDVGE
                     NYVQEAEGKFEAIGNKARWHCIGHLQRNKVKRAVEIFDIIQTVDSFKLAEEIDKRCSY
                     LSKKIPVMIEVNSGREENKAGVLPENVLQLITEISPLRSIAVVGLMTMGPFTENPEEI
                     RPSLRLTRRLFEEISKEKIEGVEMKYLSMGMSNSYRVAIEEGSNMIRLGTIIFGERQ"
     gene            complement(19976..22129)
                     /locus_tag="PKL93_07845"
     CDS             complement(19976..22129)
                     /locus_tag="PKL93_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNQ71207.1"
                     /translation="MNEIVTDIANSLSLPRWKVESAVELLEEGKTIPFIARYRKEKTG
                     ELNEIQLREISDALEYAKKLRSRKEEVLRIIEEKGKLTEELEAAINDAKNLQLVEDLY
                     LPFKSKKKTRADKAREKGLQPLADFLKTSRVKDETFIVTFVNTEQEIFQIEEALEGAR
                     DILAEEFSQEISVRERLRNQLKAKGTIKSSHSDKQDEREVYRDYYDFSQPISRLAAHQ
                     IMALFRGEREEFLSLKLELPFDILPSLRDSIGWKDYLAYYEELVEASVDSYSRLLMPS
                     IEREVRNIIKEEAEGRAIHVFARNLRDLFLQPPLGEKVVMGIDPGYRTGCKVAVIGKD
                     GSLLYHDVIYPTPPQNDYINSSMKVVQAIKTLEVELISIGNGTGSRETEVFISRLIKE
                     HKLPVKYMIVTESGASVYSASKAAIEEFPNEDVTTRGAISIARRVQDPMAEYVKIPPE
                     AIGVGMYQHDVNQSELKKTLDREVESVVNLVGVNLNTASEHLLKYISGLNSRAALNIV
                     KHRAENGGFRNRKELLKVSGLGPKAFEQAAGFCRIINGSEALDGTSVHPESYEIARVI
                     LKTAGFEPEELFTRKEQVSAKLDGIDLDAFSKDKGFNPITVREVVGMLKKPGLDPREE
                     LEKPILRTDILSMEDLSTGMELEGTVRNVVDFGAFVDIGVKQDGLIHKSKMGRKVRDP
                     LEILSVGQIVKVRVLEVDTKRMRIGLELLSNASST"
     gene            complement(22122..23066)
                     /locus_tag="PKL93_07850"
     CDS             complement(22122..23066)
                     /locus_tag="PKL93_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNQ71208.1"
                     /translation="MGEFSIVATINERRLLKSIEMSGASAIRFNSSHVSLEELQKFIS
                     YYEDNCALPLYIDLQGAKLRLSRSQPIMEVKAGEQVVLGCQSSQLRSIAVDPRTLSYL
                     SPGMRVSIEDGRIELQVLKLEADFARAMVLRGGVIRPAKGMNISPHPINQIELSSRDS
                     DIVLATRKYGFVRYALSFVSSPSEVIELKELSGREVASKIERELPFSRIEEISASSDE
                     IWFCRGDLGAQLGARGLVDFYTFFSDNLHKLYKPVLMAGEVLEHMVDTPFATRSELCH
                     LKDIQRRGFAGVVLSNETAYGSFPIETIKTVLEVTSDE"
BASE COUNT         6100 a         5094 c         5564 g         6282 t
ORIGIN      
        1 ttgagccact ttctgtattc agccagagca agcgggttgt agccgaagct tttgaacggg
       61 taccttatga agtcaagatg aacctcttcc acatcgtaat ttgagatttc cgaagcaatg
      121 cgggcagtga attctctaac atctgggata gcgggatcta tgtacggccc gaatacttct
      181 tctaaattgc tagatggata ctcaagcata gaagtgccgt tctcatctgc tgtgatccat
      241 tccggtcgcc tgttcaagat atggctttcc gaaaagggcc ttcgctttcc aaaaccccaa
      301 acaaggttta cattcatcca tgccgaaatc ctgaactccc tttcgcaagc cttttctatg
      361 gtcatctgaa gtgcatcgaa gtcttgaact aattcttcgg cgggagggag cacattcgag
      421 gagtagtaag aatcggcacg gttaacaacc tgtatgtaag ccctaactgc attaatcccg
      481 tctagctttt cgatcattct gctcagagat tcccgagata cgagttcctt gctaggaacc
      541 cacacttccg gtctgaatcc actcatcttc tcctccattc aatatgtcgg caattcagtg
      601 caaactatca gatgaagtcg atcacgttag atttttcgct tatttccggg attattatgc
      661 ccttgccgtc cgccattact accgttacgc cggggccgtg accggtaatt atacagtctc
      721 cgtgcacaac tactccgata gtaacggagc cctccatata acctccaagt ccgaaagaat
      781 cgttgtggtt ctcaagggct acaaaatcgc ctattcttag cctgtcgaga ccgactttct
      841 ttatgagtgc cctgtcattt gtaatgatgt cgtagtctcc gccggaagga tctcccgatc
      901 ctgagccgga acccatgaga taaccgggta ctattgccct aactggaaag tggatctttc
      961 catctccttc tccaacggga attctatcga gaagttctgg atcgatattg taaaccctta
     1021 tcgaaggata gtcctcaagc accaatccct ggccgaatcc gacaacctgt atcttgtctc
     1081 caatcgccat cttcttcttt atgtcttctt tgaagtgaat caagacatga tttatgccgc
     1141 catgtttgcc tattactatt cccttctccc cttttgcatc tccgctgatc aaagtggccg
     1201 aattgccgat acaggaatag ccgacatatg ccgcgttttt gtcgttgtcc gggttcttcg
     1261 tagaaacatc cggttcaaca tggtcgccca ccatcttgaa tgcgctgtcc ccaagagcga
     1321 agttataggt tattccacca gtgccgggaa agagtctggg agtcccatcg acttccagtc
     1381 tgaaggggct tctcctcaag ggatgggcaa cttctccgct taccgagatc ttcactacag
     1441 aagctttgtt tgtacgcaat gtactttcct ccattcaatc gttgtgttgt tttgaagtac
     1501 atatatatta tatcaacccg ataacgcttg tccgaaccgt gatataatcc acgctggagg
     1561 ttataagatg ttttatccgg gacatataca gaaggcaaaa agacagattc agaattacat
     1621 aaaatcggtt gatggaatag tcgagctgct cgatgcgaga atccctcttt cgagcagagc
     1681 ctatgaagca gagaaattgt ttcaaaagaa acagcgcatt gtcgttctaa acaaatccga
     1741 tctcgccgat ccgaagatca cttcgatgtg gagagactat ttcaagtcag agggaagtga
     1801 cgttgtcgaa gcctcgctga gatcgaccga cgcaaagcag ttcattataa gagaaatagt
     1861 accgttattg aaaagcagat tctacgaaaa gagattcatg gttgtaggaa tgccaaacgt
     1921 tgggaagtcg acatttatca acagactcaa aggcaagaaa tcactggccg ttggaaatag
     1981 gccgggaata actcgagggg tccagtggat aaacgtctcg gagagtatag cagttctcga
     2041 cacgccgggg atactatatt cagatctccg atctccacac ataactacga agcttcttgc
     2101 ggttggttcc ctaccgtacg agaagtttga ccctcttgac gccttcgaaa gggtgcttgc
     2161 cctaattgtc gagaggtatg ggaaaggtct cctcgaggat tacctcggtg aggaattctc
     2221 caatcaagaa gagtttgtcg agaagttctg cagaagaaga aactatcttg ccaaacaggg
     2281 ggcgctcgac actaccagag gagcccacac ttttctcagg gaggtcgctg ccgggaaggc
     2341 aggcagactc tctttcgaag atcctgaaag cttttacacc agtgattcgc cagaagcgga
     2401 gtgatttctc gatttgtttc tcaaatcgct ccatatgcga tcgatgaagc tataggtcag
     2461 ccactcttct actatgtgca gttttctcgt gccgattctg gttggtttga cgatgccctc
     2521 ctctctttgg aaatatcccc ttgctattgc ggagtgaagg agatatctca tcctcaccgg
     2581 aagccttccg aagtgttctt caagacggcg gagatctagt tgaccaccgt aaagtgccca
     2641 gaaagagtaa tagagaatcg ctgaaaactt caccattttg attgtcttac tgacaggaag
     2701 ctttccagaa tcgagaaatg agtagtattc atcgagcgaa aaggtgttgg ttcgaaactc
     2761 ctttccatta aaggaagaag cgctcgtgcc aattcccaaa aagaactcgc gtgaaacaga
     2821 gctgtatttt cttccttcgt ttctcttctt gaaggtccag agtgagtccc taatataacc
     2881 ttttgagaga gcgacgtttt ccagttcttc cagaatctcg agttccttct cgggatcgtt
     2941 cttagtgttt ttgtacgccg tatctgcgaa acgcatcatt ggatagactg atacctgttc
     3001 cgggccgtaa gaaatagccg tctcaagatc ctctacgatt cttctccgct caagctggta
     3061 atcgaagatc aagtcaacgt caacaaagtc aaagcccgcc gaagtagtcg cggctatcga
     3121 attcagcgaa acgacggaat cctgtctcct tcgccccatc gagttcaaag tgaaatcgtc
     3181 gaaggattgg atcccgagac taacgaagtt aacgccagtc tctcttattt cctcaagcct
     3241 cttgctggag acatctgccg ggtggatttc aagagcgatt tcgccatcag tcatttccct
     3301 gaaatgggag acgactccct ttagaaggtc caggtcataa gaaggtgtcc cgccccctat
     3361 gtatatggaa ctgaattgtc caactctctg tagttcggat tcgcgcttca aagccgtcaa
     3421 gtatttgttt gtcagtttag agtcgtattt gattttgttg taggggcaga agctgcaaaa
     3481 ctccgggcaa aatggcacgt gaatatacag ccctggagat gaatcgaagg ccatcgggcc
     3541 actaccatcc acgttttgta ttctgaacag cttttttcca agttccatga ctctttcggc
     3601 cagacccatt tttcgccctc ccgctccatt ctagtacgta aagtgtgtta atataatata
     3661 tgttctgagc atatatctca tcctatctgg aggtgttaca tgaaaaagga cgtagtaata
     3721 gtgggaggag gtccagccgg aatcgtcacg gcgatgacgg caaagaagac ctatcccgac
     3781 aagagcattg tggttattag aagggaacgt gaaggagtag tcccctgtgg aattccctac
     3841 atattccata ctcttccgac tgtagatgcc aataccatgc ctattaaagg cgctgaggat
     3901 ctgggaatcg attttatttt cgatgaagtt gatgagatca gcactgattc caaaatggtt
     3961 aagctctccg gtggggagtc tatcgaatat gagaagctgg ttatcgccac tggttcacaa
     4021 ccgatatttc ctccaataaa gggcagcaag cttcctggtg tctttaccat agccaaggac
     4081 agagagtatc tgaagagtgt atacgctgcc gccaaatcgg cgaagaaagt agtggtcgtt
     4141 ggtggaggtt ttataggagt tgaggtttcg gacgagatcc tctccgacgg caaagaagtc
     4201 tatctggtcg aggccgttga ccagatattg atggcggcct ttgacaagga attcggaacc
     4261 atggtaagcg aaagacttga gaagaaaggc atgaaactgc gcaccggtat gaagctgtgt
     4321 gagatcaaag gagaagacaa ggtctccgga gtcgtacttg acaatggtga agagatcgaa
     4381 gcagatatgg tgatactttc aatagggtac aggccgaacg tgaagcttct ggagaacatc
     4441 cccgttcaca ggggtataac cggtggaatc tgggccgatg aatacatgag aaccagtgtt
     4501 caagacgtct ttgcagccgg tgactgcgtc gaacacaagt gcttcttcac gagaaagccc
     4561 agtagactga tgctggcctc cacagctgct tttgaggcaa gagtggctgg ttcgaatctt
     4621 ttcagcctaa agatggtaag ggagaatcac ggaaatcttg gagtgttctc cacttcggtg
     4681 gccgggctta cggtcgctgc tgcaggtctt actgagtgta cagcaaagat ggagaatttt
     4741 gattacgtgg tcggggttgc aaagggaata gacagacatc ccggttcgct tcccgacaag
     4801 tcggaaatct ttgtcaagat ggtcttctcc aaggagagcg ggattcttct tggagcccag
     4861 atggcaggcg gaaagagtgt cggagagatg atcaatatta tcgggctagg tttgcagaaa
     4921 ggaataaccg tgaacgattt cctcacgatg cagatcggtt cccatccact actcacggct
     4981 gcccctacta gctatccgtt aactttagcg gctgaaaatg ctctaatgaa tttgagaaag
     5041 gaataagaaa cactacaaat agaagaagct acctttaggg tagcttcttt ttatgttgaa
     5101 ggatatcact ttgctttccc ctgattagcg acggcctgtt gtgcctttgc aatctcttcg
     5161 gggtcgccca gataatagtg ctttattggt tttagaccgt catccagttc atagactaga
     5221 ggtattccgg tcggaatgtt caatcctacg atctcctcgt ctgggatatt gtccagatac
     5281 ttgacaagtg ccctaaggct atttccgtgg gctactatca gcactttctt tccagacttt
     5341 atctgtgtgg atatctcatt tttccaaagt ggcaggaatc ttgccactgt gtccttaaga
     5401 cattcggttc tggggagttc ctcatctgaa agagacctgt atttcgggtc gtgacctgga
     5461 aatctctcgt cactctcttc gagcgcaggc ggccgaatat cgtagctcct cctccagatc
     5521 ttcacctgtt cttcgccatg ttttgcggcg gtctcagctt tgttaaggcc ctggagggct
     5581 ccatagtgtc tctcgttcag tctccagtcc ttgatcaccg gaatccacat gagatccatt
     5641 tcgtccatta tgtaccaaag ggttcttatt gccctcttca gaactgaagt gtaggcaaga
     5701 tcgaaatcgt atccatcggc cttcagcacc ttccccgcat tctctgcttc ctctcttccc
     5761 ttttcggaga gatcaacatc ggtccagcca gtgaaacggt tctctttgtt ccatgtgctt
     5821 tctccgtgtc ttaccagtac gagtttggtc atctttctcc tccttcctta tatctcctac
     5881 attataaact attcttcaaa ctgaggtaga tcggtccatc ttttgaaaga aaattcaata
     5941 gttcacggtc ttatttcctg ttctttgtca tccattcgac cgcgaaatct accagttcta
     6001 cttggggaat taagactgct tttccatatc caaccactcc cctattgaca gttgttggga
     6061 aggcttcttc gaaagttttt cctatcgttt caaagtcttc cgtttcaagc tcatagtcga
     6121 tgaactctct ccagactctc tttccgtttt caattatagg ggctccatct ttgcaaagtt
     6181 ttctcgatgg aaaatcggcg cggtactctg caagatgtaa agagctgtta ttccgatggc
     6241 tcacccccag gagcaatatg taagcattga gatcatacaa tctggccaga ggcgatccct
     6301 ctcccattcc aaactccagt tcgtggttat ctactatgta agaggctctc ttgcctctcg
     6361 caacgaaaga aacctggggg tgatcgctcc tgatgacacc cttcatcttt ctaaaggtct
     6421 cggggattac acccattcct ctggtggggg tttcgaccct gttgaaggga ggcattgtcc
     6481 ttctaattgg gtcgaaccag ctctctggaa cagggggatt tgtccagtta gatggctcgc
     6541 ttagatctcc ggaatgagcc ggcataacaa gagttccatc ctcgtctaga attgtttcaa
     6601 gtgcctttac tacggcaaca gcaccgccac atacccagcc cagcctgctc agggaagaat
     6661 gtactagcaa taccattccc cttttgactc caagttgttt aagactggag ataagactct
     6721 tcgtagttac cggcccgtta ttgctcagct tcactgcttc tgcttcactc atttactact
     6781 cccgaaaatc gatcacaatg aacttcatca tttcgcgatc aaccatttta tttcaatctc
     6841 ggcgcttctc ctgtctgcgg aagagtcctc aaaaatgacc ttcagattgt attcaccatc
     6901 gccttcaaag atcaattcat ttttcgtttc ttcccaatca tcggcgagac tggcgacttc
     6961 tttcccgtcc ctttccacta ccagatatgc gttgttgccg atcataactt ttttgtccat
     7021 tcgcgctata agctgctgac ccggcctgag tgagacggtg gcaaagctgt ttcccgaatc
     7081 tctggcatag agtgtcttac tctcgtagcc gtcgaatcca gccaggcaga gtgtcaagat
     7141 cacaagcccg attattccca gaattgtaaa aaaacgacct acatcgataa gggtcaaagt
     7201 cattacctcc gtagtagcct gtattagata aattataacc gaattaatcc gagatgtcgt
     7261 tcttagaacc gctgatagag cagaaatttg agactgtaat cacgtctaat taggacgatt
     7321 tcagtcgatg tataattgaa aggataatta tgagcctcat gtctagaatt aatggctttc
     7381 cgttttcaaa tattagattc gcattcacta tatccatttg gttttggaca tgagagacta
     7441 tattattgtt atagagatag ttgaaagggg gtaaactgcc ttgaagataa ttatctttgg
     7501 tggtaccgga agagtgggga gggtcattct agatagggcc ttaggcgaaa atcatatagt
     7561 cacagtcttc gttggagacg tttcaaagtt gaccgtaaga aaagagaatc ttaggatctt
     7621 ccagggggac gtcttcaaca gtcagagtgt aagggacgca ataagaggtc aggatgccat
     7681 aatcagtgcc ctcggtccag acaattcagg cagtgtcaac gataccctgg cagtagctat
     7741 gaaaaatatt gtcagtggcg caagagaaac cgaggtaaac aagatagtca cgatcgcgaa
     7801 ttccgggatt cttcagcttt cccctagaga gctaaggctc gattctccca actatcctca
     7861 gtacctgaaa aaatcttcaa gagaatttct ggacgcgttc gaaatcctca agacttctga
     7921 actggattgg gttgtcgtat gtcctccatt catgactttt tcggagggca accagagtta
     7981 cagggtttct gccgatttcc ttccagaaaa tggagtgaag atttcggttc aggatgtcgc
     8041 tgaatttgcc ttcaaacagt tgttcacctc tgagtactct cagaagagag ttggaatagc
     8101 ctattagaat ggagttatct tgaaaagaga aagccccggt tcggaaatac gtattaacag
     8161 gctggaggca cttaccgatg gtgtttttgc cattgccatg actattctcg tcctatcact
     8221 cgaagtacca agcagaagtc aggtcacgag ccaaagcgaa cttgttcaga acatcatgag
     8281 caagggttat cagtttttga gctacttcat atccttcttc gttattggat cgatctggat
     8341 ctcgactata aggagaacac acattctcaa taagaccgat tacaaacacc tttggctcaa
     8401 catgctcaat ctctttttcg tcacgacgat tcctttcact acatcccttt tgggagatta
     8461 cggcgagtat gaatttgcag aggtgctctt ccacttagat atcttgatac ttgagacaat
     8521 cgctctagtt cagtggcagt atctgatgaa taacaaggat ctgatccgtg aagatgcaat
     8581 agatgattca aatttaatct actatagaaa agtctacatc tttcatgtaa ttgttccttt
     8641 gctcggtatt gcggtctcgg tgtttactcc cgtttggagc aaccttgtct atctcgtcat
     8701 atttttcagg gtgttcttta tccgcaggta atactacgga acataaataa ttgctagaat
     8761 atatccataa tctagggggc gatggaaata gctttcacca ttattcgttt tgtagttgcg
     8821 gttctaactg ccgtaactgg tgtgtacata gtattgagac cggaatccta cgcaagactt
     8881 gccgagtttt caactggcaa ttcgagaggg agaaccgagg taaagacaat tatgggggga
     8941 acctttatcg ggctgggaat ggctccaata atcctcctgg gctcaacgat tgccttcatt
     9001 tttctgggaa cagtttattt gttcatcgct gcaactcgga ttctatcaat gtttgtggat
     9061 aagtctttcg tgttcaccaa tttgataact cttcttgttg aaatagcatt tggtctgatt
     9121 atggtgattc cggtgtgaac tgatacaaac cgacttaggt cggttttttt atgctaagtg
     9181 ttcctaccct tgtgagctcg attgaagaaa tggaggaaag gaaattgaga gaggaagatc
     9241 tgaaaagggc aatacttgaa gcatggtcaa aacgattggg tttttcatcc gatagatttt
     9301 ttcttggaac gtatttcttt tttcccgatg agagcaagag aggaaagggc gggatcgagc
     9361 aactgagctt tgaaaggcag acagtcatct tatacgatcc attgttagaa gatgttttca
     9421 agtctatcga aacccaggat gagaatccca gcgattattt ggccgatttg attcgagact
     9481 cgtttcttct tttgaagaag acttccatca aatatctcaa tccttcaaat tacgttcctt
     9541 tttacccgcc cgccgagttc actgtaagaa ggctcaatca aggggacaga acggcattct
     9601 atcgcttcag aaaccagtgc accagggagg atctggccga ggggtttgta gagcttgatc
     9661 atgtagccgt tttcggagcc ctgtacgaag acgaaatcgt tgcggtttcc agcataattg
     9721 tctgggatga aattgcagat atcggtataa ttacgatgcc tgatttcaga gagctcggtc
     9781 ttggcaaggc tctcggttct agagcgagcg agtgggctgt cataaacaaa aaattgattc
     9841 agtacaggca taacgtcttg aactatggat cgcttaaggt ttctcgcgct ctggggttta
     9901 gagagtacat tgtagatcag gaatactcat acaaaggtta gattttcttg ggtgaaagat
     9961 agaagtgaaa ggggcttttc cggtgagcga tcatctgtcc ttgatattgt aacctgtaag
    10021 tcagaacttc ttctaattag agatcgaatt gtagctttag gatttgacac agtagaagac
    10081 aaaagtgtaa aatcctttca taccagcggg gagttgagta aatgatagac ttcaggtttt
    10141 cctggataaa tgcatattct tattattata acaacagaag catttgcttg tgagtgatta
    10201 actccgggta agtgcttctt gcatttactc cggatgaaag atacgaaagg gtctccaatc
    10261 cggagaccct tttctttttc aacaggaatg cggggtggtg agatggtagt cgttcttaag
    10321 agaggtaccg gtgaagagga gattaagcaa gtggagtctc tttcagagag tctgaatctc
    10381 agctgtcatg tttccagagg attggaaaga gtggtaatag gtgtgattgg agacgaccga
    10441 tatatgtcgg tggaacgctt tgaggctctt gaatgtgttg agcaggttgt acgtgttctt
    10501 aagcctttca agctcgtatc gagagagttt cacagtgaag atatctcagt ggagatcggg
    10561 gatttgtctg ttggagaagg caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnngcgaact tggagggaac ttcaaaggcg gctttgacag aatggaaata tcttcggcgt
    10741 cgtggaaaga atcgttaagg cctcttgact gtgggaattc cgggactacc gctagactga
    10801 tggccggagt cctttcggga aaggaaggtt ttcacgttct ctttggagat gattctctat
    10861 caggcaggcc aatggagaga gtcgtcagcc ccttgaaaga gatgggggca agcatatcgg
    10921 cccgccggga ttcacttctt ccaatgtgta taactggtgg caagctaagg ggatatgagc
    10981 attctctctc agttgccagc gctcaggtca agagtgcgat tttgctggcg ggaattcaag
    11041 cggaaggtac tacggccgtt actgagccac actgcagcag ggaccacaca gagaggttac
    11101 tccgttcaat gggtgctgga attactgtga gtgataagag agttgaggta gaaaagagcg
    11161 agcttctacc ggtccggttt tcgattcccg gggacgtttc atcggccgcc tttccgatag
    11221 ctctggcggt tcttcacgaa aacggcaagg taactattag aaatgtcgga ctgaatcaag
    11281 gtaggaccgg gttcttaagg cttctcatga aaatgggtgc agacatcgag atagaggttg
    11341 aagagagctt gccagaaccg atgggcacaa tcgtaaccag atcttcccgt cttataggag
    11401 tggaggtcgg aagtgatttg attccttcaa tgattgatga actcccactg attgcgcttg
    11461 caggagtttt cgcggaaggt acaacgactg tgagaggcgc cggggagctg agaaagaaag
    11521 agtcggacag gatatccgtt accgttcaga acttcagaaa aattggcgta gagatccttg
    11581 aacatgaaga cggctttagt gtggaaggcc ctcaaagaat aactggagga aaggtcgact
    11641 catatggtga tcacaggatt gcaatgcttt tctcgatagc cggactacta agcagtgaag
    11701 gagtggaagt aactaactcg aatgcagtcg gtgtttcctt tccggagttc ttcaaaacac
    11761 tcgaggaggt ttgtcagtga agttgtgcat aattggacat ccagtttccc acagtttatc
    11821 gccggccatt tacggatggt ttttcgaaag aatgggcatc gatgcaactt acgaggcagt
    11881 tgacattctt ccagatgaat tttcagaaga gataacttca gttgcggagc atttcgacgg
    11941 gttcaacgtt actattccat tcaaggagaa gattatcgct cacgtggtaa gccaggttga
    12001 acctccgctt tcggcaataa actgcgtctt cgagagaagg ggatataaca cggactgggt
    12061 aggtttcggc aagcctttgc tcgagaggtc gatagaggag cccgtaatga ttattggagc
    12121 agggggtgct gctagggcag tgattttctt cttgaggaaa gcgggcgtga aaaggataga
    12181 actccttaac agaacgcttt ccagggcgga aaagatcaaa aaagagttcg agataccggg
    12241 actgtttgaa gtatctgtct tcccatttga gtcaatcaga gaggttgctt cgaggagcaa
    12301 gagtataatc aacgcttcct ctcttggaat gaatggagag gaaacgggga tcacctctga
    12361 agaacttgtc ggtaagagcc tcgtttacga tttgatctac tggaagacgc ccttgattga
    12421 gctttcacac gactgcggag tagaaacagt tcttgacgga agagatatgc tacttcacca
    12481 ggctctcgag aatctcaaaa tatggggtct gccttcaggc gatgtcttcg aaaagacgtt
    12541 ctgggaagtg gtgcgatgaa gtttaccgta gccggagact ctcatggcag cggcgtcttc
    12601 ggggttatcg aggagttgcc gtctggtgtc tctctttcga tcgaaaagat agatagacag
    12661 cttcaacgaa ggcagaaggg ctatggcagg ggacagagga tgaagggcga agaggatagg
    12721 gcaatcgtga agtcgggtct ttggaaggat cttacgactg gtgcccctct tttgattgag
    12781 atagagaaca aggtttccag tgccgagaag agcgtgagaa gcattccaag gcccggacat
    12841 tccgattatg ctgcgtggac tagatacaaa ctgaatgatc tggcggttta tgcagagcga
    12901 agcagtgcaa gatggactgc agcccttact gcgatcggct cagtcgcgtc tcagattctt
    12961 caagagcttg gaattacctt gtgcagctca gtcatagcga tcggaaaggt cagctgtgaa
    13021 cgtcccacag ggacagaatg gatttcgagg aattgtggat ctccggtgtt ttgccacgac
    13081 gaaatcgcct accgggaaat gttgaaggag attgataagg caaacgaact tggcgaaaca
    13141 cttgggggga gatttgttgt aatcgccgat ggaataccgg cggggaccgg aggatacggc
    13201 agtctctttg aaagacttga ttcgagaatc gggaaacact tcatggcgat tccctcagtt
    13261 aagggtatat tcattggaaa tcccgatgtg agccttcgag gaggcgaata ccacgataag
    13321 ctttacatca aagacgggat agttttcagg cagacgaata acgccggcgg tatcgagggt
    13381 gggatatcaa acggcgaacg aatcgttgtt gaggcaagcg tgaagccgat cccttcgttg
    13441 aggaggggaa tctcttccgt tgacttgagc gacttgaatg agacaaagac tccttacgtt
    13501 cgttccgaca ctacagccgt tccggcggct gcaatcgtag gagagtctat gcttgcgctc
    13561 ttgttacttg aggcgattct cgaaagattt ggaaatggtg atttctgctc gataaaaagg
    13621 agggtgaagg atgagagtat tcctcattgg aatgatgggt tcggggaaga gcacgatagg
    13681 tagaatcctc tcttcggttc tcgacaaaaa attcatagat atggattcgg agatcgaaag
    13741 ggctaagggg atgagcataa gtgagatttt tgagaaagag ggtgagaaag aattcagaag
    13801 actggagaag aaccttctga aagagctagt ccggagcgac gagattgtcg tatcgacggg
    13861 agggggtgta atactggata atgagaaccg tcagacatta aagggagaga attccatcta
    13921 tctcagactt cctccagccg atctatacag aagggtaagt gtgaagggtc gccctctact
    13981 gagggaaggg aaggaaagca ttttcaggat ttgggaagaa cgaagggagc tctatgagca
    14041 gtttccttcc gttgatactt cgagtcaaac acagtgggag accgttgcag cgatatctat
    14101 gaaaatcgcc gccggtgaaa gaaagacact taatagcagt tctcaccctg tctcaatatc
    14161 cccgggtggg ttcaaatcta taggtaggat gccgaacacg gtagtctcaa aaagggttca
    14221 aaggatcttt tccgaatgga ttccctcttc ggctttttcc atagatgacg gtgaagaagc
    14281 aaagggattt catacacttt ttcaaatcta tgaatatcta atggatcgag gagtttctag
    14341 aagtgacatg gtggtcggtg caggtggagg aaccgtaacg gatctcgttg gattcgcttc
    14401 ctcgacattt aagagaggtg ttccaataac cctatatcca acgactcttc ttgctcaagt
    14461 agatgcttca ataggcggga agaatgggct gaatttcgaa ggatgcaaga atgttgtcgg
    14521 caacttctat atgccctatg agacaatcat agatccagta gtaacgcttt cgatggacga
    14581 aggacggttt gaagaaggcc tggtagaggc cttcaagata ttccttatca cgggccgatg
    14641 gtatgagaat ttcaagagcc gatgcagaga cctgaagaat aggaatctga gagccctgag
    14701 tgagatgttg gaagaagccg taaggcagaa ggatagaata gtggcgattg atacgcgtga
    14761 aacaggaatg agaaggattc tgaatcttgg ccacacactg gggcatctct acgagccatt
    14821 agccggagtt tcgcacggca tggcggtggc gtgggggctg gaaagagaga tgcactactt
    14881 cgcaaatcta ggccttatag atgaggaaat gtacagagaa gtctcggaaa cgctatccga
    14941 aatcgtcggt cttgatttcc cacagctacc tgtcgaagat gctctaagat tacttagaaa
    15001 tgataagaag gcaacgacct cggagagcat ggagatagag attcctctcg taaggaagcc
    15061 aggttccttt gaatttgcaa aggtgagact agatgatcta ttggccgtgg tcgtatgaaa
    15121 atacttatat tgaacgggcc aaacctcaat atgcttggga tgagagaaaa aagtctgtat
    15181 ggtagcttca catacagcga acttagtgaa gccatcgaaa gaaagtgcct ggaaagcggg
    15241 gcttcaagtg aattgttcca gtccaatcac gaaggagatc ttatagagag gattcataac
    15301 attgactatg atgcgatcgt tataaatgca ggtgcactaa ctcattacag ctactcgcta
    15361 agagacgcgc ttgaattgtt tagagggatc aaaatcgaag tccacatctc aaacatcttc
    15421 gccagagaag agttcagaag caagtcggtt atctctccgg tatgcagcgg aacgatcgca
    15481 ggacttggac tgaatggata cttgcttgcg atagaatatg cagtttcaca tcttaaatcg
    15541 ttaacagatc gggaggcgac agagtgaaag ctataagggg agcaacatct attgaagcag
    15601 attgccctga agagataaga aactccgtga ttgaactcat ggaggagata ttcagcagga
    15661 ataagatcac cgaactgcac tccgtgatat ttaccgtaac taatgacata acttcataca
    15721 atccagcgac tgctttcaga aaagtgttca atcaaagcga tgttgcgctt ctgtgcctat
    15781 atgaagcctc ctttgaaaac tctcccgggg ggattatcag agtgcttctc cactgtgagt
    15841 cggagacgaa gaatttcgtt tatctgcgac gagctaaaaa tctccggccc gacaaggtgc
    15901 aggcaggaga ttcgaaggca tgaaggtcca cataatagga gccggttcga tcgggggatc
    15961 gattgcattg aggctttcga agcgcggcca tagggtttcg atctttgacc agaatgttgt
    16021 gacaacagag atgcttagga agaaggatcc gagcattgaa gtatcttcag atgcctttac
    16081 gccaggggac ctgaccgtta tcgcactacc aatgaactcc gaagaggagc tgctactgtc
    16141 ggccgatttc accggcccgg catttgatgt tgcgagcgta atgcaaccct tccaggagat
    16201 agctagactc aggaagataa gattgataag cggccatccc atggccggca acgtacacaa
    16261 gggtcctgcc ggctgggacg aatctatgtt cgatggccgc gtattcctgt tttctccggg
    16321 agagttcgca accggagagg accttggcct tgtgctgatg gtggtatctg aactggggtc
    16381 ttctcccgaa tacctcccgc cagagaggca cgattatata gtcggtagaa taagccagac
    16441 agcctatttt ctttcgagaa ctcttctgag actgggtggt gattttgaga agtattccgg
    16501 acccggttac gcttcgacct caaggcttgg aagacagaac atggatatgg ttctcgatat
    16561 ggcgagattc aatggcagga atatcgcctc gtcgcttgag gaagcggaga gttatctcca
    16621 tgacataaga actgcaatcg aggaggggga tctcagtaag cttgaagaac tgatatcggg
    16681 atattaggaa cgaagagtct ttctgaaaag aggtgtctgg agatatatga tacggtctcg
    16741 ttggtttctt gatgtactcg aactcccgca tagtttctct gaaacatcgg ataatcacag
    16801 gtttcttatc aggagatcaa tatcgtctct tccggagaac attattacgg agctatcgac
    16861 agtttccagg tcttcgaatc cgttttgaag gaaaaagctt gccagcccgt tcgaaaatga
    16921 gcttttcttc ccagatagag cttctacact gccgaaaccg cgttttctgc actcttcaac
    16981 ggaaagctga ataagctcgg aacgataatc gtatgtggag tcatgtgaat tgtagataca
    17041 cgagatgagc gcggttgaaa gggatttcgg gatcgagtat ggagaaagaa gcgagggcat
    17101 gacttcaaag aaagctacag gtatctcttc ggcataagaa acgaagccga ataggctgtc
    17161 tctaccgtta aaaccttcat accagccgat ttttttcgga gccatttccc gttccattag
    17221 tgcacacaag gcacgcatcc ctcaacgact cccttgaaag tgtttcaatc cgatcacagt
    17281 ttttcgcggg tcttctcttt gcatccccga aaggtctaac cgtctttcgc ccttcgagta
    17341 cgtcaacaat aaagtcagaa ggaattggac cggcgatctt cagatcactg taaatcaaag
    17401 taaaggggaa aataagggaa gatcggaaat agttcccgag agatcgaatt ttactaaatt
    17461 caagccgaga aagtcggcag ctattcgtgc ctgttcaaga gccagtgaat tccaggggca
    17521 ctggtcttta aaataataag ctctgatctc tttgcaagac atcgaagaac ttctctattt
    17581 ctttatgagc gagtagacag tatcccggat catctcttct ctgaaaacgt agaagcctgc
    17641 gtttttaagc cattgatcgt accagctctt caagggtctg aagttgtagt tcccgtcgtc
    17701 atctatatac tcgccaatga cgacgggaag gccgcttttc ttaagagtct cgcatctctt
    17761 ttttgactct ttttcattgc ctataccggt taccaggagt cctacaccgc tcgtcttaac
    17821 agatcgagcg atccttagaa gcgtctttga ccataattcg gggtggaggt tttgaagtat
    17881 tcctgaggcg acaaacccct caaagatgcc gagaaaatta aagtctgaaa acccaatgtt
    17941 catgactcgg attccctgtg tctgttttat agccttactg caggaaacgc ccacaactct
    18001 caatttcatt tctctcaaca agggccagaa gtcctgtgca tgacagccaa aatcggcgat
    18061 aatgttcgac tcttcaagaa agccagtcat cctaagaata aagtcctgca gctgcgactt
    18121 gtctatttct aaaccggtac aagtttgctc aagtacatct tttctatatc tttcaagcca
    18181 ttctcgtctt tgcaatctac tcacctacta tattgagatt gtatccaagc agggaagatt
    18241 atcaaacaaa agtcttgact ctaattgtca gaaatggtaa tttatcttca cctcttcagt
    18301 cttcgactct ccaaacctat aagatatttc tgctacaaaa gtgagcgatg agtctctatc
    18361 ggcctcgtaa tataaagttt ttgctaattc ctgcgaattg tctaacggta gaaagagagt
    18421 ctctctcgta tcctctcctt caacaatctt tatcgccgct tcagaaattc taagctcttc
    18481 gtctctgcaa tagtgcgtgt aaagatcaaa tgagtagtga atcctgtccg tttcaagaag
    18541 ccaggcatgt ccaatcagtt gatagcgctg aacgttgtaa agatcttgag gaatggcaaa
    18601 ttcgttgctc aatgtctctt tgtcgtcatc tataactcta acctgacccc gatatgtttc
    18661 atcaggcaag agctcgagca tggcagtaaa gacaccgttc tctgatttga gctcataaga
    18721 acgaacctct gcatcttcat cgattatatt gagaaacaac tgacttcctt cagtgaattc
    18781 agaaagagta actgaggcct tgagtgatac tttttcaaga gttgtcgaag atctggtgaa
    18841 gtgaaccgat ttgacaaaat caccggattg ctcgattcgg tttcgaatat cggctacaac
    18901 tgtatcgaga gaggcaactc tcgatttgag agtgaaaagg tcggtgcgga ctgtcgaaat
    18961 ctctcccaag agtttgtttg atgcagtaac aattgttgcg agattaacga ttgcagccgc
    19021 cgaaatcaat acaaccaata accaggcgat gcccttactc ataacatcat ctcctactga
    19081 gtattcaccc cggcaatttg aataatatca cgattttcgt cttagcttaa acacaatggg
    19141 attcgatgca atataattta ctaaaagggg gtccgaagat gattgctgag aatgtgaaaa
    19201 ggctgagaag cgaaatacct gattatgtga ccattgtagc tgcatcgaaa acgagaaccg
    19261 cttcagaaat tttggagatt ctggaaagtg gcttgaaaga tgtcggtgag aactacgttc
    19321 aggaggctga aggaaagttc gaagctatag gtaacaaagc tagatggcat tgtattgggc
    19381 acttacagag aaacaaagtg aagagggcgg tagagatatt cgacataatt cagaccgtag
    19441 attcattcaa gctcgcagag gagatagaca agagatgttc atacctgagt aagaagattc
    19501 cggtcatgat cgaagttaac agcggcaggg aggagaacaa ggccggtgtc ttgccggaaa
    19561 atgttctaca gctgataacc gagatctcac cgctcaggag tattgcggtg gtcggtctaa
    19621 tgactatggg gcctttcaca gagaatcccg aagagataag acctagtttg aggctcacga
    19681 gaagactttt cgaagagatc tcaaaagaga agatcgaagg agtcgagatg aagtacctat
    19741 cgatgggaat gtccaattcc tacagggtag cgatcgaaga gggttcaaat atgattagac
    19801 tgggaacgat tatcttcgga gagcgacaat aatgaatctg gccaactgat ctcggttgcg
    19861 atagggagtc aacggggtca atcgttcgca agatgtcttt cgagattcag aggttcagta
    19921 cctagaaaga ttagatgcga acaaagggct tcacaaagtc ttcagcctat gaagatcaag
    19981 tgctgctggc atttgagaga agttcaagtc ctatcctcat tctcttcgtg tcgacttcca
    20041 ggacccttac cttcacaatc tggcccacgc tcaatatctc tagagggtct ctaacctttc
    20101 ttcccatctt gctcttgtga atgagcccat cctgtttgac ccctatgtcg acaaaggctc
    20161 cgaaatctac gacatttcga acagtcccct ccagttccat ccccgtggac aggtcttcca
    20221 ttgaaaggat atccgttcta aggatcggtt tttcgagttc ctccctgggg tccaaacccg
    20281 gctttttcag catgcctaca acttctctca cggttatcgg attgaatccc ttatccttag
    20341 aaaaagcgtc gagatcgatc ccgtcgagtt ttgccgagac ttgttctttt ctcgtgaaca
    20401 attcctcagg ctcgaagcca gcagtcttga ggataaccct ggcaatctca taactttcgg
    20461 gatgaacgga agtcccatcg agagcctctg atccattgat gattctacag aaaccggccg
    20521 cctgctcgaa ggcctttggg cctagaccgg agaccttcag cagttccttt ctgttcctga
    20581 accctccgtt ttccgccctg tgttttacaa tgttcaacgc cgctcttgag ttcaatccag
    20641 atatgtattt gagtaaatgc tccgatgcgg tgtttagatt gacgccaact agattcacaa
    20701 cggactcgac ctctctgtcg agcgtcttct tcaattccga ctgatttaca tcatgctggt
    20761 acatgccgac accaatagcc tccggaggga ttttcacgta ttcggccatt gggtcctgaa
    20821 ccctccgcgc aatcgagatc gctcctcttg tggttacgtc ttcgtttggg aactcctcaa
    20881 tcgcagcttt tgaggccgag tagaccgaag cacccgactc cgttacaatc atatatttga
    20941 ccggaagttt gtgctccttt atcagtctgc ttataaagac ctctgtttcc ctggaacctg
    21001 taccattgcc gatagatatg agttcaacct ctaaggtttt tatggcctga accactttca
    21061 tcgaagagtt tatgtagtcg ttctgaggtg gggtcggata tatcacatca tgatagagaa
    21121 gagagccgtc ctttccgata actgccactt tgcatccagt tctgtaaccc gggtcgatac
    21181 ccattacgac tttctccccg agaggaggct ggaggaagag atccctgaga tttctggcga
    21241 aaacatggat cgcccttccc tcagcctctt cctttataat gtttcttact tctcgttcaa
    21301 tggatggcat gagcaatcta gaataggaat cgaccgaagc ctctaccagt tcttcatagt
    21361 aggcaagata gtctttccac cctatcgagt ctctcagaga aggcagaata tcgaagggca
    21421 actccagctt caacgagaga aactcttctc gttctcccct gaaaagcgcc atgatctggt
    21481 gagcggccag tcttgagatc ggttgagaga aatcgtagta gtccctgtaa acctcgcgct
    21541 cgtcctgctt atctgaatga gagctcttga ttgtcccttt cgcctttagt tgatttctca
    21601 gcctttccct cacggatatc tcctgggaga actcttcggc gagaatatcc ctggctcctt
    21661 ctagagcctc ttctatctga aatatctcct gttcggtatt gacaaaagtt acgataaagg
    21721 tttcgtcctt gacccttgat gtcttcagga aatctgccag cggctgtaac cccttttccc
    21781 tcgctttgtc cgctctggtt ttcttcttcg atttgaaggg aaggtataag tcctcaacca
    21841 gctgaaggtt ttttgcatca ttgatggcgg cttccagttc ctcggtaagt ttgccctttt
    21901 cttcgatgat tctgagtact tcttcttttc tggaacgaag cttctttgcg tattcgagtg
    21961 catcggagat ttctcttaac tgtatctcgt tcagttcacc ggtcttttcc tttctgtatc
    22021 tggcaatgaa tggaatagtc tttccttctt ccagcaattc aacggcactt tccactttcc
    22081 atctcggtag agagagcgag ttagcaatgt ctgtaacaat ttcattcatc cgatgtgacc
    22141 tccagaaccg tctttatggt ctctatcggg aaggaaccgt aagccgtttc gtttgaaaga
    22201 accactcccg cgaatcctcg cctttgaatg tcctttagat gacaaagctc actcctggta
    22261 gcgaagggtg tatctaccat gtgttcaaga acttcaccag ccatgagcac aggtttgtag
    22321 agtttatgga gattatccga gaagaatgta tagaagtcta cgagacctct cgcacctagt
    22381 tgggctccga ggtcacctct acagaaccag atctcgtcac ttgaagccga gatctcctct
    22441 attctggaaa acggaagctc tctttcgatc ttggaagcta cttctcttcc ggagagttcc
    22501 ttcagttcga ttacctcgct tggactggaa acaaaggaga gcgcatatct aacgaagcca
    22561 tacttccttg tggcaagcac gatgtctgaa tctcttgagg agagttcaat ctggtttatc
    22621 ggatggggag agatattcat acctttggcc ggtcgaataa cgccgcctct cagaaccata
    22681 gctcttgcaa aatctgcctc gagtttgagt acctgcagtt caatcctgcc gtcttctatt
    22741 gagaccctca ttccgggaga gaggtatgaa agggttctgg gatcaaccgc tatgcttctc
    22801 agctgagagg actggcagcc aagaacaacc tgttcaccag ccttcacttc cattatcggc
    22861 tgacttctgg acagccgaag cttggcaccc tgtagatcga tgtacagagg aagagcacag
    22921 ttatcctcat agtaagagat gaatttttga agctcttcaa gagaaacgtg agaggaattg
    22981 aaacgtatgg ccgatgctcc cgacatttca atcgacttga gaagtctacg ttcatttatt
    23041 gtagcaacga ttgaaaactc tcccatctag atcacctcta aagagattat atcagctacg
    23101 ggatttgaga actcagcgtt ttcaaaacga tgaaacatct
//
LOCUS       DAOQYI010000028        20450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_2983_length_20450_cov_71.318608, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000028 DAOQYI010000000
VERSION     DAOQYI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 20450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20450
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2983_length_20450_cov_71.318608"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PKL93_07855"
     CDS             complement(<1..498)
                     /locus_tag="PKL93_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNQ71209.1"
                     /translation="MKALVFDGNLKLLDVPLPVRRPGTSLVKVNLAGICNTDLEITRG
                     YMDYHGILGHELLGTVIESESAILSGKRVTTEINIPCKTCDLCKQGLFKHCRNIKTVG
                     ISGYPGVFAEYAVLPDENLHVIPESVSSLKAVFTEPLAAAAQVFESVELSPDQRVCLI
                     GDGKLG"
     gene            complement(495..1691)
                     /locus_tag="PKL93_07860"
     CDS             complement(495..1691)
                     /locus_tag="PKL93_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNQ71210.1"
                     /translation="MDKTKKLHFDTLSIHAAEQEDQNQSLNPPIYMTSTFTFTDLQQA
                     EDTFSFKRRAYVYTRGGNPTINLFEQRLATLENGVDGVAFSSGMAAISSVVMSFAAAG
                     DSLVAHRNLYGSTFGFLNHLITNYGVKTKFINMTDLNAVEKAITSETKLIYLETPTNP
                     ALEIIDIEALAKIAHSNGVKVVVDNTFATPVFQRPLDFGADVVLHSATKYISGHGDVI
                     GGFATSKDFDYVQKLKFGYMCELGGVMSPFNAWLLLRGMKTLGLRMERHEKNAREIAN
                     FLRNRPEVVKVSYPGFEDHPGHDIASRQMEGFGGIISFELKGPRENAESFVRNLKLIK
                     LAVSLGDAETLVEVPAMMTHRDYPEEELHEFGFSNKTVRISAGLEHYSDLLNDIEDSL
                     DKVYRQ"
     gene            complement(1865..2575)
                     /locus_tag="PKL93_07865"
     CDS             complement(1865..2575)
                     /locus_tag="PKL93_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71211.1"
                     /translation="MTGILLLLVGSATRFLLSPLWFFLACTPFYIAFTVLRKRFDYRV
                     LVLAPFVPIVAEIIAIFSGNFRAGFLFGDVICIITVLLGMGDERPKEKMTRELGLSGP
                     INRRRVSSVFYTFGRVAQIEKVNMSQSYSIVHENAFHFTVDVPGTGRVKMTVPLEEIE
                     EVSVHMSMSPGELYLPSLRDMFLPAKKIKMIGKPKIKDYFLLVKTSEDTYSFYEEPST
                     VLRIQEEIEEAKKKISVT"
     gene            complement(2581..3900)
                     /gene="mnmE"
                     /locus_tag="PKL93_07870"
     CDS             complement(2581..3900)
                     /gene="mnmE"
                     /locus_tag="PKL93_07870"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNQ71212.1"
                     /translation="MFNDPICALSTPRGLSATAVIRCSGKGIVSRTSHLLPDLKTLKP
                     RHAHLTSFMEGGRYIDEVVVVFFQGPASYTGEDLLELSFHGNPLIIENALESLFRVGF
                     RMALPGEFTKRAVLNGKFDLVKAEAINALIASKTEKSLEAAIKSFRGSLSEEVASFRE
                     RMVRLLASVEVELNYPDEIETDYSSLYEDLVRLKKEMGEFIEKSRNGVVISQGIKTAI
                     VGETNVGKSTLLNALLKRDRAIVSEMPGTTRDTIEEDLNVGGVLFRVIDTAGIRQAEN
                     EIEELGIERSLKAIEEAELVILLRDPHNPESKDLEEELRNKGKRLIVAANKSDIRKVE
                     QHHFDVVISARTGEGLKDLEKLMLKRTEEITSIGDEVIISARQKQKLLDAVDYIARSI
                     EAIEDNITVDVLSTMIEQAARNLDELLGTHITEDVLERIFSDFCVGK"
     gene            complement(3890..6928)
                     /gene="mfd"
                     /locus_tag="PKL93_07875"
     CDS             complement(3890..6928)
                     /gene="mfd"
                     /locus_tag="PKL93_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNQ71213.1"
                     /translation="MERVLYRVNEPISWVEKGNYNASRNLIIFPSERMLDSFIEIHER
                     REGDGFFFSHDVFPFEEIGTSSRIRSERLALLRKLLLGELRTVYTSFHGILRKTVPIE
                     VFEELSFKVEVGGPMTISEDQLQSLGYSRSFSVTIPGEFAIRGGIVDVFIPGSEMPVR
                     IDTFDREIESIRSFDPVSQKSLQRLNEVLITPAAEGITASPFKETALKRIRAAEQATG
                     VSDEILLARLDTMDTTAGIFYERQSILLDFLEGYSVIFVKPDDAIVEYGRRERETLEL
                     LSEKAVRKFLYIRYGGVSSEVLMKLNEYSIVSDGDVSSLDYDEELTEELQIIKRPRRE
                     EEYLPRIPVVDWTELEEGDYVVHKEYGIGRYLGVRTVENILGTREYLLLEYRDGNKIY
                     VPVDRVDRVHKYIGSTEGIQLNSLRGTAWTRQKSKVNKEVKALISDLSNLYGSREVTS
                     GIPLTGDSEMEKGFRDSFPYVETEDQQKAVQEVMEDLQSTKPMDRLISGDAGYGKTEV
                     ALRAAFRTVVSGKQVAVMVPTTVLARQHYENFERRLNPFGIRVEILDRYRTDVQRNKL
                     LKKLKKGEVDVVVGTHSLLSKEVGFADLGLVVVDEEQLFGVLQKEHFKKLRLQVNVLS
                     MSATPIPRTLYMSLSGLRDLSIISTPPAGRTSPEIYVGQINDRLIRTAVLRETNRGGQ
                     TIFVHNRVTELQELLSRLRDLLPEVKIDVAHGQMNKTAFERTIRDFYLGELDMLLCTT
                     IIESGVDVPNANTLIVDDSHRYGLAQLYQLRGRVGRSNRRAFSYFLYDPKRLSGPSRE
                     RLKALKEFSGAGSGMKIAMRDLEIRGFGTLLGAEQHGNINSVGLYLYREMVEKAMREL
                     HGEEEIGVEERTVDTELKNIPFDMVIPEEYVSDSIERLKIYRRIAACKIQDEIDEIEL
                     ELLDRFGRLPSQVNSLLEASRVRLGAFNIGIKIVEYDPHSESIVMLHGENHIRDSLGI
                     KGRRLVVNEREGKSILYGVPERHLMRTLKSLFLGAERNVQ"
     gene            complement(6904..8130)
                     /locus_tag="PKL93_07880"
     CDS             complement(6904..8130)
                     /locus_tag="PKL93_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNQ71214.1"
                     /translation="MKAAILLTGDEITKGVVKDSNGGYLSEKLTALGFEVQSIVIVPD
                     RKEFIEREISNALDKADLLLITGGLGSTFDDITLQTVADFLGKELIYDSEYHSSLSRS
                     FEERYAREAPAGLKRQSFVIQDSIQLANPSGSARGAYIREDNKEIVILPGPPVEMEAV
                     FREALKFITLPPPSYSRTIGFVDLTEPEVEEFTEQMLTEFPSVKFVTRVRYFSGPTVI
                     LTSDNESILEEICSLFTRRWKKYVYTTTGEELVQVVVKELKARKLSVSVAESCSGGRL
                     SALLTSVSGVSDVFPGAIVSYSNDIKISLLKVNEDTLNRHGAVSEEVAAEMAAGTKIL
                     FGTDIGLSVTGIAGPTGGTETKPVGMVCSAVRIGDLTKTYTDMFRGDRETIQLRGAST
                     VLKRLWRLFWNEFYIE"
     regulatory      8177..8277
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8308..9285
                     /locus_tag="PKL93_07885"
     CDS             8308..9285
                     /locus_tag="PKL93_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ71215.1"
                     /translation="MRRALLLSLMIFFLSLSAVSALTVYSYDSLEPLAEESFGKFTEQ
                     TGIDVEFRAVGDSGSLLSLIISEREKFDGDVVIGIDNLLSRRAFEEDIFLSYRPMGFE
                     RIISNDLLLDEEWRLTPYDFGSIALICNGSQIEESISTLWDLTKPEFERSVIIQDPRT
                     SSTGLSFLAWTYLLFGERFEEFWRAITPSILTVSLSWDDAFQKFESGEAPIMVSYATD
                     GAYSKHYYGESIYSTVIPEGKGYVQVEGAGIIKWTDNLEEAKLFMDFILSEDFQSHIP
                     LNQWMFPVIEVEMPPAFDYAVKPQKILKLSADVDLSKLIETWEEVIYER"
     gene            9329..10855
                     /locus_tag="PKL93_07890"
     CDS             9329..10855
                     /locus_tag="PKL93_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ71216.1"
                     /translation="MPLFLAFGNLNFETIFSVVSSPSFKSIAGFTVKQSLLSTLLAMI
                     VGFPAAVHYARSSGRLSSLLGITTYIPFFFPSISMAIGFLSVYGRNGIFNNFLSSLGF
                     ERVQILYSLGAVLLGHVFYNAPIVILVLGNALRKLPSDVLESSKIDGSGRIMRLLRVE
                     LPLVTPAIINSALLIFTYCFTSFAVVLILGGAQFATLEVSIYMNFRLLAAPQNAVVLA
                     VVQFIFIMLFGLLISASEKSATFEQGEQYKEKNRFTAIYSWTFLLFEWLPILGALLSS
                     FYDWTRGEFIFSRISKLFGEKLVLIGAGLTNTLINSLTLSALAALVTVTIAFLLSWQA
                     RKTIKGSKQLRIISLIALSVTPSILAVSYLTVFGGFPVPILILLLYIVISLPVVLNYI
                     SGQVIGAELNFLEAAQIDGASKIERLRYMIIPFFRSTIIYAATVVFAISMGEFGGSLI
                     LGSKDFPTFAVAIYRLNGSRYLLEARFLSSALGIIIISVVSVSRWFAQKPERSIRNTG
                     "
     gene            complement(10785..11447)
                     /gene="nth"
                     /locus_tag="PKL93_07895"
     CDS             complement(10785..11447)
                     /gene="nth"
                     /locus_tag="PKL93_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNQ71217.1"
                     /translation="MSRNDCSPEAVSSWIVENFPREQEYGDPFKVLVSTILSQRTRDE
                     NTEEASRRLFSVYPDPQSLIDAKPEDLYDLIKASGMYRQKAARIINCARMIVESFAGE
                     VPDTLEELVTIPGVGRKTANIVLNVSFKKEALAVDTHVHRIANRLGWVKTKTPDDTEF
                     ELMKILPPSIWGPVNGSMVEFGREICRPIGPKCNLCGISQCCEYFSLVSEQTTERQKQ
                     QK"
     gene            complement(11440..12858)
                     /locus_tag="PKL93_07900"
     CDS             complement(11440..12858)
                     /locus_tag="PKL93_07900"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNQ71218.1"
                     /translation="MKREDAKKLSNKLTMRKRSAWFSLDKEEVFAYSKEYMKFIGKSV
                     TERLAVKNLVDLLESRGFKPVSSFEKTGVKRGDRIYLTKDNKALIATVIGEDLKEGID
                     MVAAHLDAPRLDLKPSPLVEDSEIAMLKTHYYGGVKKYQWLNIPLAFVGTVIKSNGES
                     VDVSIGLNSGDPVFVISDLLPHLDNREGDFRKVFQGKDLNAMSGTIPIVEGKDFENPV
                     KLMFLNLLYEKYGLVEEDFFSADIQLVPALEPREVGLDRSMIGAYGHDDRVCSYSALT
                     ALADMSSIDNPKRTAMVLLFDREEIGSEGVTGAKGQFWVTFIEKLLNLSGAKGLYEID
                     FLLEKSRMLSGDVAAGFDPTFKDAHDQANVARFGYGIAILKYTGSRGKSGTSEASAEF
                     MGYVRRLLNQKGVHWQSTILGEVDRGGGGTVAKFFAERGVTVVDAGTPVFGMHSPFEL
                     LSKADLYETYRAYKVFLEGGDV"
     gene            13113..13682
                     /locus_tag="PKL93_07905"
     CDS             13113..13682
                     /locus_tag="PKL93_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ71219.1"
                     /translation="MVWLDEKKLIDGLKKKKVWAYEVLYDEYAPRLGSVVKSYLGYDD
                     VEDVIQEVFIKVFKNVRKFRGDAKLSTWLYRIAVNVCKDTLVKRKRNNEFLTDFRESE
                     DKVAFEPPATTNVFREVMDEISFEELMSVVERLAEDDRLLIKLRDIDDLSYEEIADIL
                     DKPVGTVKSRLHYARKRLKELLEEVGYIG"
     gene            13675..14169
                     /locus_tag="PKL93_07910"
     CDS             13675..14169
                     /locus_tag="PKL93_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71220.1"
                     /translation="MDEERFVRILDREIPFDKLRVDERKELKGYVKAIDALKESCCYK
                     PSDELKKRTLSNVRKLKSRPYKILGTVAACLVLGIFSVSFFTGNTILIKKKGELYQLA
                     DVLGSSSSLNERLRVNLNAFTQGEAEGNNLVMMEKEMERSLGFLSESAVENDFEISGI
                     STTY"
     gene            14209..15090
                     /locus_tag="PKL93_07915"
     CDS             14209..15090
                     /locus_tag="PKL93_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71221.1"
                     /translation="MKKLSVLVVLLLVVGLAVGKDAVEELTTLVDVLKNSSYEVDRYV
                     QESGIVTTAKNERVIKSGPYKLVTSYSSAKGEFGWVRNSSGHFAIFRTRSIAFEPLTK
                     IKDVEDNFIDLVNRKNYVVDLFLDLPNSRKITISSGNLTFEATYDDNYKLLKLVKSYP
                     FHTISASYRGYSEMSHEALTKLSNATEGMIIIRPPIYFSEAMLEKHFAWSSMDFATDG
                     KTYLYTVLMYSIEYGRMVLYFSFNTEPDYLQKFSAQMAQANGYSYAIERLSENSAISL
                     LGMIDTETLSSLLEDLY"
     gene            15203..15631
                     /locus_tag="PKL93_07920"
     CDS             15203..15631
                     /locus_tag="PKL93_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNQ71222.1"
                     /translation="MEIKDTVALLKSKGYRITPQRVAIIRILRDHEGHPGVEEVFRSV
                     IQVHPNISMATVYNVMEVLEKEGIIKAIANSKRSRRYDSDVKPHGHFMCNSCGRVFDI
                     PTEYYEACLKVLPSEMIDFEVNSFELTIKGICPDCKKKLL"
     gene            15661..16692
                     /gene="pheS"
                     /locus_tag="PKL93_07925"
     CDS             15661..16692
                     /gene="pheS"
                     /locus_tag="PKL93_07925"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNQ71223.1"
                     /translation="MKGLDINLQEVLERLMKISDLNELQEEKSRLTGKQGVITSLMKK
                     LKEIPPEERREYGKTVNEYKNFIEEALNRRKSELENKAKKEREASERLDYTIPGKKRD
                     IGSLHLITQIMEEVQSIWVSMGFEVTVGPEIETSYYNFEALNTPEWHPARDMQDTFYL
                     QDGRLLRTHTSPVQIRTMESRKPPLAIFAPGTCYRKDTPDATHLPMFHQFEILFVDKG
                     VSVSHLKGSLDHFAKRLFGEDRKIRLRPSFFPFTEPSFEVDVSCGICGGVGCRSCGGS
                     GWLEILGAGMVHPNVFRNVGYDPEVWSGFAAGTGLERIAMLKYDLEDIRDLLRNDRRF
                     LRGYRRAVI"
     gene            16689..19058
                     /gene="pheT"
                     /locus_tag="PKL93_07930"
     CDS             16689..19058
                     /gene="pheT"
                     /locus_tag="PKL93_07930"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNQ71224.1"
                     /translation="MKLYLEWINEFVDISDKSPQELNNSLSLSGTEVAGIEYPWSYVQ
                     GAVVGKIKSARPHPMSDRLLVTRVDAGVRELTIVTADLTVKEGETVGVLPEGSVLSGT
                     KIENKEFLGVLSEGMFLSLEELELEEKSQTIMRLEQGKPGESVIELLGLDSAAIEVEL
                     TANRGDCLSVVGMARELGAIYERKVERPCVDDSIPVGDDRLLKVTLKDEGCTRYTALL
                     MNDVKICDSPLWMKRRLVAAGLRPINNVVDITNYMMLETGHPIHAFDVDRIGSTEIVV
                     RSARNGEKLELLDGKVVQLDSSDMLITNGETPLALAGVMGGEDSGIYSSTKRVLLEVA
                     VFDPVRIRKTSRKLGISTDSSYRFERGIDYEDSIYVIRRLADLMERLSGASVASKIVD
                     AGGTVPVKAIDLREEFVTDRLGGIVPSEKIGAILESLGFKTVRTDFGWKVTPPAFRSY
                     DTRQEIDLVEEIGRIYGYDKIDNVLPRVLPISGGVPEFLKRQKRVKDLMVANGFDEIV
                     TYSFMNPDEFDKLGTDPKHVALLNPLSMDMAVMRPSLVYNLLAAASYNYRRQNRDIKL
                     FEIASVFNPEREQSETTALGFVATGRENPLDYSDKRILDFFTFKGVIDELCSLYGLDP
                     VYNPLTISWLEKGKAVSVDVDGTEIGFFGAVDISAADALYEIKSGEVYVGELNISTIE
                     SLMKGFSGAKKISPFPRVFRDLSFLVPGSIAYGELEELIRRTLDGTSFEEIRVSDLYR
                     GKGIEPGYKSITVTLAFESFDKTLTDDEINTLIELVLRETEKIGVKLRG"
     gene            19051..19836
                     /locus_tag="PKL93_07935"
     CDS             19051..19836
                     /locus_tag="PKL93_07935"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNQ71225.1"
                     /translation="MDNLLLNNFSKKIRLYLPELWRKISKEEFSIERKSGYKDIVTSV
                     DLEIEDNLRVFLHDLYPEAAYLGEESGPDVRSSTMWIVDPVDGTTNFSKSNPHYSTQI
                     ALYSEGRVVLGVTYDHNRRETFHAIEGQGAYLNNKRIWVSKTRELREATSHVGMQYSS
                     ETAFERITRRINRAIKICRGLRITGSACLDFAYVASGRADIFWEETLKPWDVASGILL
                     VKEAGGFVGSCVEEDFDLFNPNVLVANNSPELVKEFRDRVLYD"
BASE COUNT         5251 a         4731 c         4803 g         5665 t
ORIGIN      
        1 acccagtttt ccgtcaccaa tcaggcagac tctctgatcc ggtgagagtt ctacggactc
       61 gaagacctga gcggccgctg ccagaggttc ggtaaatact gccttcagac ttgaaacaga
      121 ttctggaatc acatgaagat tctcgtctgg aagtaccgcg tactctgcaa atacccccgg
      181 gtagccggag attccaacgg tcttgatgtt tctgcagtgt ttgaaaagcc cctgtttaca
      241 gaggtcgcag gttttacagg gaatgttgat ctccgtagtt acacgtttgc ctgacaagat
      301 ggccgattcg ctttcgataa ccgtccccag aagctcgtga ccgagaattc catggtagtc
      361 catgtagcca cgggtgattt ccaagtccgt attacagatc ccggcaagat tgacctttac
      421 caaagatgtt ccgggccgtc ttaccggtaa tggaacgtcc agcagtttaa ggttaccatc
      481 aaagacaagc gctttcattg tctgtaaacc ttgtcgagag aatcttcgat atcattgagt
      541 aaatccgagt aatgctcaag tccagcagaa attcgtacag tcttgtttga aaagccgaat
      601 tcgtgcagct cttcttccgg ataatctctg tgagtcatca tcgctggcac ctccaccagt
      661 gtctcggcat caccgagaga taccgcgagt ttgattagtt tcagatttct aacgaagctt
      721 tcagcgttct ctctcggacc tttcaattcg aagcttatga tacctccaaa tccttccatc
      781 tgccgcgatg caatatcatg tccgggatga tcttcaaagc caggataaga taccttcact
      841 acttcggggc ggttcctcag aaagtttgcg atttcccttg cgtttttttc gtgtctctcc
      901 attctaaggc ccaatgtctt catacctcta agcaacaacc aggcgttgaa gggactcatc
      961 actcccccca gttcgcacat gtatccaaac ttgagctttt gaacgtagtc gaagtccttt
     1021 gaagttgcaa agccgccgat tacgtctcca tgaccggata tgtacttcgt tgcgctgtga
     1081 agcacgacat ccgcgccgaa atcaagcggt cgttgaaaga cgggtgtcgc aaatgtattg
     1141 tcgacaacta ccttgacacc gtttgaatgt gcgatcttag cgagtgcttc tatgtcgatt
     1201 atctcgagtg caggattggt aggagtttca aggtagatta gctttgtctc cgaagtaata
     1261 gctttctcta cggcgttaag atcggtcatg tttatgaatt tcgtcttgac cccgtagttc
     1321 gttattaggt gatttagaaa accgaatgta gagccgtaga gattcctgtg ggcaaccaac
     1381 gaatccccag cggcggcaaa ggacatgaca accgagctta tggctgccat gcctgaagag
     1441 aaggctaccc cgtcaacacc gttttccagg gttgccagcc tctgttcgaa aagatttatc
     1501 gtagggttcc cacctcgtgt gtatacgtag gcacgtcgtt tgaatgaaaa agtatcctct
     1561 gcctgctgca ggtcggtgaa tgtaaacgta gaggtcatgt atattggtgg atttagagac
     1621 tgattttggt cttcttgctc tgcggcgtga atcgagaggg tatcaaagtg tagcttcttc
     1681 gtcttatcca taataaacac ctcgcttcct ttatgacatt atatcgtagg aaagaaggag
     1741 cgccggtaac attaagtatg aagctagtaa aacaagtatg ttattgatag aatcgttagc
     1801 cgacaaatat ctctacttgt atcgaccgct gtattaataa cggacgtcga gggaaggcct
     1861 ttcttcaggt cactgagatt ttcttctttg cttcttcaat ttcctcttga atcctcagta
     1921 ccgtcgacgg ctcctcatag aaagagtaag tatcctcaga agtctttacg agcaggaagt
     1981 agtctttaat ctttggcttt cctatcatct tgatcttctt tgcgggaaga aacatatccc
     2041 tcagagaagg aagatacagc tcaccggggg acatgctcat gtgaaccgag acctcttcta
     2101 tttcttcaag cggaactgtc atcttcactc tccccgttcc gggcacatcg accgtgaaat
     2161 ggaaggcatt ttcatggact atcgaatagc tctggctcat atttactttt tcgatctggg
     2221 cgactcttcc gaacgtgtag aacaccgaac tgacccttct tctgtttatg ggtcctgaca
     2281 ggccgagctc ccttgtcatc ttttccttgg gacgctcatc tcccattccc agtagaaccg
     2341 taattatgca gattacatca ccaaaaagaa atccggctct gaaattgcct gaaaaaattg
     2401 cgattatctc tgcaactatg ggaacaaacg gagccaggac cagaactcga tagtcgaacc
     2461 tctttcttag aaccgtaaag gctatataga acggtgtaca ggcaaggaaa aaccatagag
     2521 gcgaaagcag aaatcttgtc gcagatccga ctaaaagcaa aagaattcca gtcatcgaaa
     2581 tcacttcccc acgcaaaaat cgctgaagat tctctccagc acgtcttccg ttatgtgtgt
     2641 tccaagaagt tcgtccagat tcctagctgc ctgctcgatc atcgtgctta aaacatcaac
     2701 ggtaatgtta tcttcgatgg cctcaataga tctggcaata taatcgactg catctaagag
     2761 tttttgcttc tgcctcgcac tgatgattac ctcgtctcct atggaagtaa tctcctccgt
     2821 tctcttcaac ataagcttct ctagatcttt caaaccttct ccggttcttg cgctaataac
     2881 aacgtcgaaa tgatgctgct cgacttttct gatgtcggat ttgttcgcgg cgactataag
     2941 cctctttcct ttgtttctca attcttcttc gaggtctttg ctctcagggt tgtgtgggtc
     3001 gcgcagcagt attaccagtt cggcctcttc gatggccttg agacttctct caattccaag
     3061 ctcttcgatt tcattctcgg cttgtcttat tcctgccgtg tcaattacgc ggaaaagtac
     3121 cccgccgacg ttgagatcct cctcgatcgt atctcttgta gttccaggca tttcggagac
     3181 gatcgctcta tctcttttca aaagggcatt cagaagagtc gatttaccga cattcgtctc
     3241 ccctacgatt gcggtcttta taccctgaga gatcacaaca ccgttcctgg atttctctat
     3301 gaattcaccc atttcctttt ttaagcgcac caggtcttca tagagcgaag agtagtcggt
     3361 ttcaatctcg tcgggataat tcaactcgac ttcaactgag gcgaggagac gaaccatcct
     3421 ctccctgaat gaagccactt cttcggaaag agatcctctg aagcttttga ttgcagcttc
     3481 aagagacttt tcggtcttag aggctatcag ggcattgatc gcttcggcct tcacaagatc
     3541 gaatttcccg ttcaggactg ctctctttgt gaactctccg gggagggcca ttctgaaacc
     3601 cactctgaat aatgattcaa gcgcgttctc aattatgagg ggattgccgt ggaaggaaag
     3661 ctcaagtaaa tcttctccgg tgtatgaagc aggtccttgg aagaagacga cgacaacttc
     3721 atcgatgtag cgaccacctt ccataaatga ggtcaagtgt gcatgccttg gtttcaaggt
     3781 tttcaagtcc ggcagcaggt gacttgtcct tgaaactatc ccctttccgg aacatctaat
     3841 tacagcggta gcagaaagtc cgcgcggtgt tgaaagtgca catataggat cattgaacat
     3901 ttctctcagc tccaagaaaa agcgatttca gtgttctcat aagatgtctt tccggcacgc
     3961 cgtagagtat cgatttgccc tcgcgttcat ttacaactaa ccttcttcct ttgattccca
     4021 gtgaatcccg aatatggttt tcaccgtgta acataactat actctctgaa tgtggatcgt
     4081 attctacgat ttttatccca atgttgaagg cgcctagcct aaccctggaa gcttcgagaa
     4141 gcgaattgac ctgagaggga agccgaccaa atctatccag aagctccagc tcgatttcat
     4201 ctatctcgtc ctgaattttg catgcggcta ttctccggta gatcttcaat ctttcaatgg
     4261 aatcggaaac atattcctcg gggataacca tatcgaatgg aatgttctta agctcagtat
     4321 cgacggttcg ctcctctact ccgatctctt cttcaccatg aagttccctc attgcctttt
     4381 ccaccatctc gcggtagaga tagagtccaa cggagtttat gtttccatgt tgctccgctc
     4441 ccaataaggt tccgaatcct cttatttcca ggtcccgcat ggcgatcttc ataccacttc
     4501 ccgctccgga gaactccttc aaagccttca gtctctctct cgacggaccg gacaatctct
     4561 ttggatcgta cagaaagtac gagaatgccc ttctgttgct tctgccaact cttcccctca
     4621 actgataaag ctgggcaagt ccgtatctat gagagtcatc gactataaga gtattggcat
     4681 taggaacgtc gactccgctc tcgattatcg ttgtgcacaa aagcatatct agttcgccaa
     4741 gatagaagtc tctgattgtt cgctcaaatg cggtcttgtt catttgcccg tgggcgacgt
     4801 ctatcttaac ttcgggcaga aggtctctca atcgagagag taactcctgc agctcggtca
     4861 ctctgttgtg aacgaagatc gtctggccgc ccctattggt ctctctgagg accgcggttc
     4921 tgatcagtct gtcgttgatc tgtccaacat agatttctgg tgaggtgcgt cccgcaggcg
     4981 gtgtggagat tatcgaaaga tcccgcagac ccgaaagaga catgtaaaga gttctcggaa
     5041 tcggagtcgc gctcattgag agaacgttga cctgaagacg gagcttcttg aaatgctctt
     5101 tctgaaggac tccgaaaagt tgttcttcgt cgaccaccac gaggccgagg tcggcaaacc
     5161 ccacttcttt cgaaagtaga gagtgtgttc ctactacaac atcgacttcg cccttcttga
     5221 gtttcttcaa aagtttgttt cgctgtacat cggtccgata acggtcgaga atctcgactc
     5281 ttattccaaa gggattcaac cggcgttcga agttttcgta atgttgtcgg gccagtaccg
     5341 tagtgggaac cattacggct acctgttttc cggagaccac cgttcggaac gcagcgcgta
     5401 aagcaacttc ggtctttcca tagcccgcgt caccgcttat gagtctgtcc ataggtttgg
     5461 tgctttgaag gtcttccatt acttcttgta ctgccttttg ttgatcttct gtctcgacat
     5521 acgggaagct gtcacgaaag cccttctcca tttcgctgtc tccagtgagt ggaatgccgg
     5581 aagtgacttc tctagatccg tagaggttcg acaggtccga gatgagggcc tttacctctt
     5641 tgttgacctt agatttctgt ctcgtccagg cagtcccgcg caaggagttt aactgaattc
     5701 cttcggtgct tccaatatat ttgtggaccc gatcaaccct gtctaccggt acatagatct
     5761 tgttcccatc tctgtattca agtagaagat attctctcgt tcccagaata ttctctaccg
     5821 ttctcactcc aagataccgc ccgatgccat actccttatg gactacgtag tctccctctt
     5881 caagttccgt ccagtcgaca acgggaattc tcggtaagta ctcctcttct cttctcggtc
     5941 tcttgatgat ctgaagttct tcagtcaatt cttcgtcgta gtctagagaa gaaacgtcgc
     6001 cgtcggaaac gatcgagtat tcgttgagct tcatcagcac ttccgacgaa actcccccgt
     6061 acctaatgta aaggaacttt cttaccgcct tttcggaaag gagttccagt gtttctcgtt
     6121 ctcttcttcc atattcgacg atcgcatcgt cgggcttcac aaagataacg ctgtagcctt
     6181 ctagaaagtc gagaagtatc gactggcgtt cgtagaagat gccggccgtt gtgtccatgg
     6241 tatccaatcg ggctaagaga atctcatctg agaccccagt cgcctgctcg gccgccctga
     6301 ttcgcttcag tgcagtttct ttgaaaggcg aagccgtaat cccttctgcg gccggagtaa
     6361 tcaacacttc gttcaacctt tgtagactct tctgggagac cggatcgaaa ctcctgatgg
     6421 actcaatttc cctgtcgaag gtgtctatcc tcaccggcat ttccgatccg ggtatgaaga
     6481 catctacaat tccacctctt attgcgaact ctcccgggat agtaactgag aatgatctcg
     6541 aatatccgag actctgaagc tggtcctcgc tgattgtcat cgggccgcca acttcgacct
     6601 tgaaggacag ctcttcgaaa acttcgatcg gtacggtctt tctcaggatt ccgtgaaaag
     6661 aagtgtaaac cgttctcagc tctccgagga gaagctttct gagcagggct agcctttccg
     6721 atcttatcct cgaagaagta cctatttcct caaatggaaa cacatcatga gaaaaaaaga
     6781 agccgtctcc ttcacgtctc tcatggatct caatgaagga atcaagcatt ctctcacttg
     6841 ggaagatgat caagtttcgc gaagcattat aatttccttt ttcaacccag gagattggtt
     6901 cattcactct atataaaact cgttccaaaa taacctccac aatctcttta agacggtgct
     6961 cgcacctcta agctgaatag tctctctatc ccctctaaac atgtcggtat aagtttttgt
     7021 taaatcgccg atcctgactg cagagcagac catacccacg ggcttagttt ctgtgcctcc
     7081 agtcggtccg gctattcccg tgacggaaag accgatgtct gtgccgaaaa ggatctttgt
     7141 tccggccgcc atctcagcgg caacctcttc tgaaactgcc ccatggcgat tcagagtatc
     7201 ttcattcact ttcagaagtg atatcttgat gtcgttggaa taggagacaa ttgcaccggg
     7261 aaaaacatcg gaaacccctg aaaccgatgt tagaagagct gagagacgcc cccccgaaca
     7321 ggattctgca accgagacag atagttttct agccttgagc tctttcacta ctacctggac
     7381 caactcttcg ccggtcgtgg tatacacgta tttcttccat cgtcttgtga ataacgaaca
     7441 gatctcttca aggatagatt cattatccga agtgagaatg acagttggcc ccgagaagta
     7501 tcttactctc gtgacaaact tgacgcttgg aaattccgtg agcatctgct ccgtgaactc
     7561 ctctacttcg ggttccgtaa ggtcgacaaa accaattgtt ctagaataag aaggcggtgg
     7621 gagcgtgatg aacttaagcg cctctctgaa caccgcttcc atctccactg gaggtcccgg
     7681 aagtataacg atttccttat tgtcttctct tatgtaagct cctctcgcgc ttcccgatgg
     7741 atttgctagt tgaatggagt cctgaatcac aaaagactgt cttttcaacc ctgcgggagc
     7801 ttctcgagca tacctttctt caaaacttct cgagagcgac gaatgatact cgctgtcata
     7861 aatcagctct ttcccaagaa aatcggcaac cgtttggagc gttatgtcgt cgaaagtgct
     7921 tccgagtccg ccagttatta gcaagagatc ggctttatca agagcgttac tgatttctct
     7981 ttctatgaat tctttcctgt cgggaacaat gactattgac tgaacttcga atcccagggc
     8041 agtgagtttt tcagaaagat agccgccgtt actgtctttg acaactcctt tagtaatctc
     8101 gtcgcctgta agaaggattg cagccttcaa atgaccacct cctcgtcaat tgtatcacca
     8161 aacggacggt ggtataattg attgccgggg gagcccaaag ggctgagagt gctgaaagca
     8221 gaccccatga acctgatccg gttaggaccg gcgtagggac ggccagtgag tgcgcctccg
     8281 gtaatcattt acaggaggtg tatttttatg agaagagcat tactgctctc actgatgatc
     8341 ttcttcctct ctctttcagc agtatcagcg ttgactgtct actcatatga ttccctcgaa
     8401 ccgcttgccg aagagagttt tgggaaattc acagaacaga ctggaattga cgtcgagttt
     8461 cgtgcggtgg gagactcggg ctcactgttg agcttgatta tttcggagag agagaagttt
     8521 gatggagatg tcgttattgg gatcgacaat cttctttcgc ggcgggcctt cgaagaggac
     8581 atatttctct cttatcgccc catgggcttt gagagaatca taagcaacga tctcctgctg
     8641 gatgaggagt ggcgactcac gccttatgac tttggaagta tagcgctcat ctgtaacgga
     8701 agtcagatcg aagagtcaat ttcaaccttg tgggacctta ctaagccgga gttcgaaaga
     8761 agcgtaataa tccaggatcc gagaacctcc agtactggtc tctcatttct tgcatggact
     8821 taccttttgt ttggagagcg cttcgaagag ttctggcggg cgataactcc gtcgattcta
     8881 acggtttcgt tgagctggga cgacgccttc cagaagttcg agtcgggaga ggctccaatc
     8941 atggtttcct acgccaccga tggagcgtat tcgaagcact actacggaga atcgatctac
     9001 tcaaccgtta tacccgaggg taagggatat gttcaggtcg agggagcggg gataattaag
     9061 tggactgata atcttgaaga agcgaagctc ttcatggatt tcattcttag cgaagatttc
     9121 cagagccaca tccctttgaa ccagtggatg ttcccggtta tcgaagtaga gatgccgccg
     9181 gccttcgatt atgcggtaaa accccaaaag attctaaaac tctcagccga tgttgatctc
     9241 tccaagctga ttgaaacgtg ggaagaggta atttacgaaa ggtaagtcac taacaatata
     9301 ctcagtcgct atcgcggccg ttgttctttt gccgctgttt ctcgcatttg gaaacctaaa
     9361 ctttgaaacg atcttctctg tggtttcatc gccttcattc aaatcaattg cgggattcac
     9421 tgtaaaacag tctttgctaa gcactttgct tgcaatgatc gtaggtttcc ccgcggcggt
     9481 acactatgcg aggagcagcg gacggctttc aagtttgctg ggtataacta cgtacatacc
     9541 attcttcttt ccaagtattt ctatggcaat tggcttcttg tctgtatatg gcagaaatgg
     9601 aatcttcaac aacttcttgt ctagtttagg gttcgagagg gtacagattc tttattcttt
     9661 gggggccgtt ctccttgggc acgtgtttta caatgcgcca attgttattc tcgtgctagg
     9721 gaacgctctt aggaaactgc cctcggatgt tctcgagagt tcaaagatag acggatctgg
     9781 aagaataatg aggcttctcc gcgttgaact gcctctcgtt acgccggcaa taatcaattc
     9841 ggcactgctc atcttcactt attgttttac ctctttcgca gtagtactta tccttggcgg
     9901 tgctcaattc gccactcttg aagtctcgat ctacatgaat ttcaggcttc tggctgctcc
     9961 tcaaaacgca gtcgtgcttg ccgtcgttca gttcattttc atcatgttgt ttggattgct
    10021 catctcagcg agtgaaaaaa gtgcgacttt tgagcagggc gagcagtata aagagaaaaa
    10081 cagatttaca gcgatttact catggacttt ccttcttttc gaatggcttc cgattcttgg
    10141 tgcgctcctg agttccttct acgactggac gagaggggaa ttcatattca gtagaatttc
    10201 aaagctcttt ggtgagaaac tcgtactcat tggagccggc ctgacaaata ctctgatcaa
    10261 ttcgctgaca ctctctgccc ttgcggcgct agtgaccgta acgatagcct ttttgctttc
    10321 ctggcaggcg cgaaaaacca taaaaggctc gaaacagctt cggataattt ctttgatagc
    10381 actctccgta acaccctcta tactggcagt atcgtatctt actgtgttcg gtggttttcc
    10441 ggttccaatc ttgattctat tgctctatat tgtgatttcc ctgccggttg ttctaaatta
    10501 tataagcggt caggttatcg gagcagaact gaacttcctt gaggcggcac agatagacgg
    10561 cgcctcaaag atagagcgtt taagatacat gatcattccc ttcttcagaa gcacaattat
    10621 atatgcggca accgttgtct tcgcgatctc tatgggtgag ttcggtggat cgctcatact
    10681 tgggagcaaa gactttccaa cctttgccgt tgcgatctac agactcaacg gcagccgcta
    10741 tcttctggag gcgagatttc tcagttcggc gttgggaata attatcattt ctgttgtttc
    10801 tgtctctcgg tggtttgctc agaaaccaga gagaagtatt cgcaacactg gctgattccg
    10861 cagagattgc acttaggacc gatcggtctg cagatttctc tgccgaactc gaccatcgat
    10921 ccattcaccg gtccccatat actaggtggc aagatcttca tcagctcaaa ttcagtatca
    10981 tcgggagtct tcgttttcac ccatcccaac ctgttggcga ttctgtgtac atgtgtgtca
    11041 acggccagtg cctccttttt gaaggaaacg tttagaacga tgttggccgt ctttctcccg
    11101 actccaggga ttgtcaccag ctcttcgaga gtatccggca cttcgccggc aaagctctcc
    11161 acaatcattc tggcgcaatt gataattctt gcggctttct gtctgtacat ccccgaggcc
    11221 tttattaggt cgtagagatc ctcgggtttt gcgtcaatta aggattgagg atctgggtaa
    11281 accgagaaga gcctacgaga ggcctcctcg gtattttcgt ctcttgttct ttggctgaga
    11341 attgtgctta ccagaacctt gaacgggtct ccgtattcct gttcacgcgg gaaattttca
    11401 actatccagc tagaaacagc ttcgggagag cagtcgtttc tagacatcgc ccccctccag
    11461 gaaaaccttg tatgctctgt aagtttcgta aagatctgct ttggagagaa gctcaaacgg
    11521 cgaatgcata ccaaagactg gcgttcctgc atcgactacg gttactcctc tttcggcaaa
    11581 gaactttgct actgtaccgc caccgcctct gtcaacctct ccgaggatcg tgctctgcca
    11641 atgcactccc ttctggttaa gcagtcttcg cacatagccc atgaattctg cgctggcttc
    11701 gcttgtcccc gattttcctc tagaacccgt gtatttgagg atagcgattc catagccgaa
    11761 tctcgctacg tttgcctggt catgagcatc cttgaaagta ggatcgaagc cggctgcgac
    11821 atccccggaa agcattctcg atttctccaa aaggaaatct atttcgtaaa gcccttttgc
    11881 ccccgagaga ttgaggagct tttcgataaa cgttacccag aactgtccct tcgctccggt
    11941 gactccctcg cttcctattt cttctctgtc aaagaggaga accattgccg tacgcttagg
    12001 attatcaatc gaggacatat ctgcaagcgc agtgagggca gaataggaac agactctatc
    12061 atcatgacca taagctccga tcatggatct atccagtccg acttccctgg gctcaagagc
    12121 gggtaccagc tggatgtcgg cagaaaagaa atcctcttcg acaagtccgt acttttcgta
    12181 gaggaggtta aggaacatta gtttgacggg attctcgaaa tcctttccct caacaatagg
    12241 tatcgtgccg gacattgcgt tcaagtcttt accctgaaat acctttctga aatccccttc
    12301 cctgttgtct aggtgtggaa ggaggtcgga aataacgaag acgggatctc cactgttcaa
    12361 tcctatcgag acgtcaaccg actctccgtt actcttgatt acagtcccca caaaggcaag
    12421 aggaatgttg agccactggt atttcttgac tcctccatag taatgagtct tgagcattgc
    12481 gatttccgaa tcttcgacca acggactggg cttaagatca agccttggcg catctaggtg
    12541 agcggcaacc atgtcgatac cttccttgag gtcttcgccg atcactgtgg cgataagcgc
    12601 cttgttgtct ttcgtcagat aaattcggtc tccccttttc acgcccgtct tctcaaatga
    12661 tgatacgggc ttgaaacctc tcgattcaag aagatcaacg agattcttca ctgccaggcg
    12721 ctctgtaaca gatttgccaa tgaatttcat gtactctttc gagtaagcga agacctcttc
    12781 cttatcaagc gagaaccagg cactcctttt cctcatcgtt agcttattac tgagcttctt
    12841 tgcatcttcc cgtttcaatt cttcacctcc agtaatgcga ataagttctt tcacctttaa
    12901 ttttatccga tttgattggc tttttcgagc tcagtgattt gctttacaaa atttccgttt
    12961 ttgcttgcag atggtcttgt ttttcatgat tcgctaatca aaagattcgc agtctcttcc
    13021 ccggtgttat gattgattta aggataatta tataattaac gtgaggagtt gaactgtatt
    13081 cgctccttta tcgtcatata gaacagatta gggtggtatg gttggacgaa aagaagctaa
    13141 tagatggact aaagaagaag aaggtctggg catacgaagt cttgtacgat gaatatgctc
    13201 cgaggttggg aagtgtcgta aaatcatacc ttggatacga tgatgttgag gatgttatcc
    13261 aggaggtttt tataaaggtc ttcaagaacg ttaggaagtt tcgtggggat gcaaaacttt
    13321 cgacctggct ttatagaata gctgtgaatg tctgtaagga tactctggtg aagaggaaga
    13381 gaaacaacga atttcttacg gatttcagag agagtgaaga taaggttgcg tttgaacctc
    13441 cggctacgac aaatgtgttc agagaagtca tggacgagat ctcttttgag gaactgatgt
    13501 ctgttgttga gagacttgct gaagatgatc gactacttat aaagttgaga gacatagatg
    13561 atctgtcgta cgaggagata gccgacatac tcgataaacc ggtcggcacg gtcaagagca
    13621 gattgcatta cgctcgaaag cggcttaaag agcttctcga ggaggtaggt tacattggat
    13681 gaagaaagat ttgtccgcat tttggatcga gagatcccgt ttgataagct gagggtagat
    13741 gaaaggaaag aactaaaagg ctatgtgaag gctatagacg ctctgaaaga gagttgttgc
    13801 tacaagcctt ctgatgaact gaagaaaaga actctgagca atgtaaggaa gctaaagagc
    13861 agaccttaca aaattctcgg taccgtagct gcctgtttgg tactgggtat cttttcggtt
    13921 tcgttcttca ctggtaacac tattcttatt aagaaaaagg gagagcttta ccagctcgca
    13981 gatgtcctag gcagtagctc gagtctgaat gaaaggctca gggtcaatct gaatgccttt
    14041 acgcagggcg aagcagaggg gaataatctg gtaatgatgg agaaggaaat ggagcgttcg
    14101 ctcggctttc ttagtgaatc agctgtggag aatgattttg agatctctgg aatcagcacg
    14161 acctactaat ctatagacgc tctaagacac agaaggagtg aaatcgccgt gaaaaaactg
    14221 tccgtactcg ttgtcctgtt gcttgttgtc gggctggcgg ttggaaagga tgctgtcgaa
    14281 gagttaacta ccctggtaga tgttttgaag aactcttcgt atgaagtaga tagatacgta
    14341 caagagagcg gaatagttac tactgcaaag aacgagcgag taataaaatc cggtccctat
    14401 aaactggtta catcgtattc ttctgcaaag ggtgaatttg gctgggtgag aaacagctca
    14461 ggccattttg ctatctttcg cacaaggagt attgctttcg agccgttaac aaaaatcaaa
    14521 gatgtggaag acaacttcat tgacctggtg aacaggaaga actatgtggt cgatctcttt
    14581 ctagatcttc ccaacagcag aaaaatcacc atatcatcgg gcaatctaac tttcgaagct
    14641 acttacgatg acaattacaa gttgttgaag ctggttaagt cgtatccatt tcatactatt
    14701 agcgcgagtt acagaggtta ttcggaaatg tctcacgaag cgttgacaaa gttgtcaaac
    14761 gcgactgaag gcatgataat aatccgtcct ccaatctact tttcagaggc aatgctggaa
    14821 aagcactttg cctggtcatc aatggatttc gcaactgacg gaaagacata tctttacact
    14881 gttctgatgt attcgatcga gtatggtcga atggttctat acttctcatt caataccgaa
    14941 ccggactatc ttcagaagtt ttccgctcag atggcccagg caaacggtta cagttacgcg
    15001 attgagagac tctcggagaa ctctgcgatt agtcttctcg ggatgataga tacagaaaca
    15061 ctctcttccc ttcttgaaga tctctactga atgcgagcgt tcactaaaag taagacttat
    15121 tgaggttttc ataaagattt ctcaaaattg attgaattct gttgtataat caactcaaac
    15181 cctttatgga ggctttaatt gtatggaaat aaaggatacg gttgctcttc taaaaagcaa
    15241 aggttacaga attactcctc agagagtcgc gataataagg attctaaggg atcatgaagg
    15301 ccatcctgga gtcgaagagg tttttagatc ggtaatacag gttcatccga atatttcgat
    15361 ggcaaccgtt tacaacgtaa tggaagttct cgaaaaggaa ggaatcataa aggctatagc
    15421 gaattcgaag agatcgagac gttatgattc agatgtgaaa cctcacggcc acttcatgtg
    15481 taatagctgt gggagagtct tcgatattcc gacggaatat tacgaagctt gcttgaaggt
    15541 gcttccttct gaaatgattg acttcgaggt gaattctttc gaacttacca tcaagggaat
    15601 ttgtccagac tgcaagaaaa aacttctata gacaagtttt atctttttgg gggtgctgga
    15661 gtgaagggac tcgatattaa tctacaagaa gtcctcgaaa gattgatgaa aatatccgac
    15721 cttaatgagc ttcaggaaga aaagtcacgg ctcactggaa aacaaggcgt gataacttct
    15781 cttatgaaaa agttgaaaga gattcctcct gaagagagaa gagagtacgg aaagaccgta
    15841 aacgagtata agaatttcat tgaagaagct cttaacaggc gtaagagcga actggagaac
    15901 aaggctaaaa aagaaaggga agcctctgaa agactcgatt acacaattcc cggaaagaaa
    15961 agagacatcg gttctctaca cttgataacg cagataatgg aagaagtgca gagtatctgg
    16021 gtcagtatgg gtttcgaagt gacagtgggc ccggaaatcg agacctctta ctacaacttc
    16081 gaagcactta atacccccga gtggcatccc gcaagagaca tgcaggacac attctatctt
    16141 caggacggaa gacttcttag aacgcacacc tcgcctgtgc agataagaac gatggaatca
    16201 agaaagcccc ctctagcaat tttcgcgccc gggacatgct acagaaaaga cactcccgat
    16261 gcgacacacc tgccgatgtt ccaccagttt gaaatcctct ttgtggataa gggtgtttcg
    16321 gtgagccatc taaagggctc tctggaccat ttcgccaaga ggctctttgg agaagacagg
    16381 aagataaggc tgcggccaag cttcttccct tttaccgagc cgagtttcga agtcgatgtc
    16441 agctgcggaa tttgtggagg agttggttgc agaagctgtg gtggaagtgg gtggctagaa
    16501 attctggggg ccgggatggt gcatccgaac gtattcagga atgttgggta tgatccggag
    16561 gtctggagtg gctttgctgc cggaacgggc cttgagagaa ttgctatgct gaagtacgat
    16621 ctcgaagata tcagggatct tcttcgcaat gacagacgct tcctcagggg ttacaggagg
    16681 gcagtaatat gaaactgtat ctcgaatgga ttaatgaatt cgttgacata agcgacaaat
    16741 ctccccagga attaaacaac agcctttcac tcagcggtac ggaagtggcg ggaatagagt
    16801 atccatggag ctatgtccag ggtgcggtgg tagggaagat taagtcagca agacctcatc
    16861 ccatgagtga tcgacttctg gtaacaaggg tggacgcagg ggttcgtgaa ctaacaattg
    16921 ttactgccga tttgaccgtt aaggaaggcg aaaccgttgg cgtgctgccg gaaggctccg
    16981 tcctctccgg tacgaagatc gagaacaagg agtttctagg agttttatct gaaggaatgt
    17041 tcctttctct tgaagagtta gaacttgaag agaagtcgca aacgataatg cgacttgagc
    17101 agggcaaacc tggagagtcc gtcattgagt tgctgggact cgattcggcg gctattgaag
    17161 tggagttgac tgcaaatcgt ggcgactgtc tctcagttgt tggaatggct agggagctcg
    17221 gtgccattta tgagagaaag gtagagagac cctgtgttga cgactcaatc cccgttggag
    17281 acgaccgact gctaaaggtt acattgaaag atgaaggctg cacaagatat acggcacttc
    17341 taatgaacga tgtaaaaatc tgtgattctc cattatggat gaagagaaga ttagtagcgg
    17401 ccggtttgag accgataaac aatgttgttg atataactaa ctacatgatg ctcgaaactg
    17461 gccatcctat tcacgctttc gatgttgatc ggataggatc tacggaaata gttgttcgtt
    17521 cagcaagaaa cggtgagaag cttgagcttc tggacggcaa agtggttcag ctcgacagta
    17581 gcgacatgct tattaccaac ggtgaaacac cactcgctct ggctggagtc atgggcggtg
    17641 aggattccgg aatctattct tcaacgaaaa gagtcctttt agaagtcgcc gtcttcgacc
    17701 cagtgagaat ccgaaaaaca tcgagaaagc taggaatctc aacagatagt tcgtatcgat
    17761 ttgaaagagg aatagattac gaagattcta tatacgtgat tcggcgcctt gccgatctca
    17821 tggagaggct tagtggtgcg tcggttgcct cgaagattgt cgatgccggc ggaactgttc
    17881 cagtcaaggc catagatttg agagaggaat ttgtaaccga tagactgggt ggcatagttc
    17941 cttccgaaaa gataggcgcg atacttgaga gtctgggttt caagaccgtg agaaccgact
    18001 tcggatggaa ggtcactcca ccggctttcc ggtcctacga tacgagacag gagatcgatc
    18061 ttgttgaaga gataggacga atctatggct acgacaagat cgataatgta ttgcccagag
    18121 ttctgccgat ttcgggaggt gttccggagt tcttgaaaag gcagaagaga gtgaaagatc
    18181 tgatggttgc caatggcttc gatgagattg tgacatacag tttcatgaac ccggacgagt
    18241 ttgacaagct tggaactgat ccgaaacacg tcgctctctt gaatcctctt tcaatggaca
    18301 tggccgtcat gagaccatct ctcgtctaca acctcttggc agcggcttca tacaattaca
    18361 gaagacaaaa cagagatata aaactcttcg aaatagcttc tgttttcaat ccggaaagag
    18421 agcagagtga aacaacggca ctgggttttg ttgctaccgg gagggagaac ccacttgatt
    18481 attcagacaa aagaatactt gatttcttca ccttcaaggg agtcattgat gagttgtgct
    18541 ctctatatgg tttggatccc gtgtacaatc ccctgacgat ctcctggctt gaaaagggca
    18601 aggcagtatc ggtggacgtt gacggaacgg aaattggatt ttttggcgct gtggatatct
    18661 cagctgccga tgcgctttat gagataaaga gtggtgaagt ctacgtagga gaactcaaca
    18721 ttagcacaat cgagagcctg atgaagggat tctccggagc gaagaaaatc tcgccattcc
    18781 cgagggtttt tagagatctg tccttccttg ttcctggctc aatagcctat ggagagctgg
    18841 aggaattaat aagaagaact cttgacggta cctctttcga ggagatcagg gtctctgacc
    18901 tttacagagg caaaggaatt gaacctggat acaagagcat cacagttacc ctggcttttg
    18961 aatcctttga caagactctc actgacgatg agatcaatac actcattgaa ctcgtcttaa
    19021 gagaaaccga gaagattggt gtgaagctac gtggataacc tccttctcaa taatttcagc
    19081 aaaaagattc gtctgtatct tccggaactc tggagaaaga tctccaaaga agagttctct
    19141 attgaacgca agagtggata caaggacata gttacctctg ttgatctgga gatcgaggac
    19201 aacctgcgtg tcttcctgca cgatctttat cccgaagcag cctatctcgg tgaggagtcc
    19261 ggtcccgatg taagatcttc tacaatgtgg atcgttgatc cagttgatgg aaccacaaac
    19321 ttctcaaaat ccaaccccca ttattctact caaatagctc tatacagtga aggccgagtt
    19381 gttctcggag ttacctacga tcacaacagg agggagacct ttcatgcgat cgaggggcag
    19441 ggcgcttatc ttaataacaa aaggatctgg gtgagtaaga cgagagaact ccgagaagcc
    19501 acgagccatg tggggatgca atactcgtct gaaactgctt tcgagcggat tacaaggaga
    19561 ataaatagag caataaagat ctgcagggga ctgcgaataa ccggaagtgc ctgtctcgat
    19621 tttgcttacg ttgcgtctgg aagagccgac atcttctggg aagagacact taaaccctgg
    19681 gatgtcgcaa gtggcatttt actcgtcaag gaagccggag gttttgtcgg cagctgtgtc
    19741 gaagaggatt tcgacctgtt caatccaaac gtcctcgttg cgaacaactc accggaactt
    19801 gtgaaagaat tccgcgatag agtcctgtac gactgaacgc aatttatttt tcaacctgga
    19861 gaattcattt cagactgaac attccgtcac aatcatgcag tgataactaa cgcttatttg
    19921 aatgctcggc tatactgcga tcctttgaaa aggagtttga gcaataaatg ctccactctc
    19981 aagaaattac gcgcttattg ctgtgcaaaa gaaaaaagca gcctacttct taaggcaatt
    20041 gcttccccct tcccgctatg tctcttgccg gagctctcga taatggtctt tctcgcctga
    20101 tcttttggac ggaacgagat gctgaaacaa gttcagaacg acgcgaaggg caggctatcg
    20161 actacggggt atggggtctc gtgaagagaa ccggcaatca attcagggtt tcaatgcaac
    20221 tatggaagtt gtcccgacat cggtttgtgg gaatccctcg ctttgtcatc ccggacttgt
    20281 ttcgggatct aggttttgat cctctcggcg gaggacagcg aaccgttgac agacaacgcg
    20341 gttttcatcg gcgtttatcg gcgcttagat ggagatcctg tgcagaaaca ctcacaggat
    20401 gaccctgaac gggagcacga aacacaggca tctgttgcag gttatacggg
//
LOCUS       DAOQYI010000029        20255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_3014_length_20255_cov_76.178218, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000029 DAOQYI010000000
VERSION     DAOQYI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 20255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20255
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3014_length_20255_cov_76.178218"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1505
                     /locus_tag="PKL93_07940"
     CDS             168..1505
                     /locus_tag="PKL93_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ71226.1"
                     /translation="MKRVNILNENMMSALFKLAWPAVLTMLFQTVYNMVDAYWLGKLG
                     KIEISAPTIAWPAIFLLISIGGGLAVAGLALVSQNLGAGRKEEATHVAGQVISTSFAV
                     AVVLGLLGAIFSEAVLRLLSIPPELLSVTNIYMRTIFLGAPFTFTMFTFNSLFTAIGD
                     TKTPMYLMGFSVTVNALIDPLLIFGIGFPRLEVFGAALATVISRGVVVIIATVILFKG
                     KRGFKINLRDLRPKWKTLVRVLRIGLPSSAGQSITALAFLIITSMVAGFGSVATAALG
                     VGNRITSLATMFSFGLSQATSSMVGQYLGAGRKSDAYSVVWKATGINVLIVGVISTGT
                     FFFGKEVTAFFINDPLVLIEGEKYFRIVSFSIPIFASYNIFDMALRGSGHTVQSMILN
                     ITRLWGIRLPLIYFFGMSMGITGIWYAMFVSNLVISSAAAIVIFMKRWLNPVI"
     gene            1805..2908
                     /locus_tag="PKL93_07945"
     CDS             1805..2908
                     /locus_tag="PKL93_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71227.1"
                     /translation="MRKLFVILLVVFLSVSALASIKVGAILPMTGGVAAFGQMIWQGV
                     ELANELFPEVLGEKIEIVLLDNNSDKVQAVNTARRAIEQEGVVAIIGQVISSNTIAGG
                     TVAEENGVAMVSPSSTNPLVTQDKKYVSRVCFSDPFQGVAAAMLAFNNMGAENVAVFV
                     DVEQDYAVGFANYFKETANELGGSVFYEYYKSGDQDFTAQVSDAISKGAGAFFIPGYY
                     QEIALIAIQARQLGFYEPIIAGDGAAVPETIEIGGDAVNGLYFTTHYDAGSPAITENA
                     KLFVEAYTAKYGEAPGTFTALGFDTYLVVRDAIERAGSTDREAIARAVRQTKYYPAVT
                     GIITIDENGDAIKSVAIVKIENGKFVYDTTINP"
     gene            2974..3915
                     /locus_tag="PKL93_07950"
     CDS             2974..3915
                     /locus_tag="PKL93_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ71228.1"
                     /translation="MDPKTLLQNFINGLSLGSLYALIAIGYTMVYGILRLINFAHGDI
                     FMMAVYFALFFVTLAQLPWYLAAILAVACAALLGFTVDRIAYKPIRNAPRISALITAI
                     GVSFFLESLAVVVFSGIPRSFRTIFPQSLNEMIIIGGEMEVKYGREVIVGGIRFPVIS
                     LITLIVAAIALVFLWWFIFKTKVGMAMRAVSLDIQTTSLMGVNVDRVIGMTFALGSAL
                     AGIGGILWAIRYPQVWPYMGFIPGMKAFVAAVFGGIGSVPGAVLGGLILGITEVMMVG
                     IMPGAAGYRDAFAFFILIIVLSIKPSGLLGRPEIVKV"
     gene            3920..4966
                     /locus_tag="PKL93_07955"
     CDS             3920..4966
                     /locus_tag="PKL93_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ71229.1"
                     /translation="MKKRTKMILTLIALPSIALLLFLSQELLNSYYSRIITLIGIYGI
                     MAVSLTLVNGISGVFSLGHAGFIAVGAYTSALLTLSPEQKEMTFLIEKLVWPLNSIQI
                     PFFWATIIAGLVAAVFAFLIGWPSLRLTGDYFAIATLGFSEIIRIFALNLNSITNGAL
                     GLKALPDHTNVWWAWGWLLVTVATVLSLAYSSFGRALRAIREDRVAAQAMGINVFKHQ
                     LTAFVLGGFFAGISGSLYGHWLSTIDPRTNTFGILLTFNVLIMIVLGGLGSITGAIIG
                     GALFAFLSEWLRFLEGPMNILGFKIMGISGMRMLVFSGLFVIIMIFWPRGLMGRSEFS
                     WDGLVSVFKRRYKR"
     gene            4963..5757
                     /locus_tag="PKL93_07960"
     CDS             4963..5757
                     /locus_tag="PKL93_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71230.1"
                     /translation="MSLLTLENVTMKFGGLTAVKDVTLRVEEGEIFGLIGPNGAGKTT
                     IFNMITGHYKPTDGRILFRENEITALQPDKITRTGIARTFQNIRLFRDLTVLENVMVS
                     QHHTIASNGKAVSWFFKSVTRLGYSEKEREMRNKALELLSVLGLEMLANEKSSALSYG
                     SQRLLEIARAMATGATLLLLDEPAAGMNASETAGLVDTIRRIRDDFGLTVLLIEHDMK
                     LVMGLCERIMVLDHGRTISEGDPAFVQKDVRVIEAYLGREWVTVGK"
     gene            5747..6463
                     /locus_tag="PKL93_07965"
     CDS             5747..6463
                     /locus_tag="PKL93_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71231.1"
                     /translation="MANEILSVDELQVSYGVIKAVKGITLSVEEGSIVTIIGSNGAGK
                     STTLGAVSGLIRPSGGKVSFRGNNINRLGAARTAKLGISLVPEGRRIFSNLTVRENLL
                     MGAYNRKDKEEIEGNFEAVFSLFPVLKERLKQSGGTLSGGEQQMLAIGRSLMANPDLI
                     MMDEPSLGLAPIVVEEVFQAIQTLNSRGVTILLVEQNAAKALEISKYAYVLETGKITL
                     QGSAKELLESEEVRKVYLGI"
     gene            complement(6475..7104)
                     /locus_tag="PKL93_07970"
     CDS             complement(6475..7104)
                     /locus_tag="PKL93_07970"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNQ71232.1"
                     /translation="MEILFSALLAYLIGSIPTAFLIGKSLYGFDIRKQGTGNMGASNM
                     VAVKGWKIGILTGAIDFMKGLGTILIIKGVSPHWTYQMLFIAASFAAIGHMYPVFLRF
                     KGGKGTATLAGGLIGLDYQVALICMVILLAFIAITDYAATGIIAVSSVMIFLFRIFGY
                     RWGISLLLIPIAVIMIARNLENIQRILLGEEPGLRAHIRKKKRAPQKGA"
     gene            complement(7112..7885)
                     /locus_tag="PKL93_07975"
     CDS             complement(7112..7885)
                     /locus_tag="PKL93_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNQ71233.1"
                     /translation="MTYVILFSSLVVLAYMSLFFAIGTLKKDNSVVDIGWGAGFVVVA
                     LFTLFVYGEFNLRQVVTTILIAFWGIRLTTHIFKRNWGRGEDFRYVQMREKWGEKVLI
                     RSFLQIYMLQGLFMVIISYSVMLINSHSGRSFGFLDVLGILIWACGFAIEAIADKQLR
                     DFVKTKKPGEIMTKGLWRYSRHPNYFGEAVQWWGIFVIALSIEGGIWAIISPITITIL
                     LRFVSGVPYLERKYKQYPAFREYMKETNAFVPWFSRKKS"
     gene            complement(7895..9712)
                     /locus_tag="PKL93_07980"
     CDS             complement(7895..9712)
                     /locus_tag="PKL93_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNQ71234.1"
                     /translation="MSSKFLNLLDGTMFFNAFSSGATAVIREQNDLNRINVFPVPDGD
                     TGTNLASTMRSILELTDVSHSIGKTTRSIADAALMGARGNSGAIFAQYMHGLSTIIGD
                     RDSIDRKTFAKAAHEAISYAYDAMVTPVEGTMLTVMKDWAEALVAIEDSERSFFEMVE
                     RSNEVALKSLEETPEKLTVLKKAGVVDAGAKGFVAFIEGIKTFLSGRGVNLEDEIIPD
                     IELKKHVHIDEGNIKNRYCTEAIIEGSRLDVNEIKSIISQFGDSALVAGTDTKARIHV
                     HTNEPAELFFKLKDFGTVTQQKVDDMEMQYRVSQKRKYPIALVVDSVCDLPKEVIDYY
                     QIQMVPLYLNFGKSQYLDKITLKADQFYTLLDTTEDYPVSAQPGLNTFQNLYHFLGTH
                     YDSIIAIHVSDKLSGTWNASTKAAERMKSKKISVINSRHVSGSIGLLALTAARMIEEG
                     RSHDEIAETIESLSKNTRIFVSVNTLKYMVKGGRVSPVLGLVGKAMNLKPIVSVDKEG
                     NSKLYGKAFSKLGNMKKILGFVEELNKKCSVVSYNITHAHAHKTAKAYEESLMKLLGK
                     KPEYIMEVSPVVGISSGVGAVSVSVLCERDTWSPDVNIK"
     gene            complement(9844..10044)
                     /locus_tag="PKL93_07985"
     CDS             complement(9844..10044)
                     /locus_tag="PKL93_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNQ71235.1"
                     /translation="MTGTVKWFNGTKGYGFITKDDGGDVFVHFSAIEMDGFKTLDEGQ
                     RVEFEVEDGPKGPQAAKVRIAK"
     gene            complement(10220..11530)
                     /locus_tag="PKL93_07990"
     CDS             complement(10220..11530)
                     /locus_tag="PKL93_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71236.1"
                     /translation="MVGFIFYFGRTKAGCSIYFILPVLLFTITILLIGVLRTDVVQSL
                     SALGSIVGVILSMDRSVRLAVPGLFMVGVSLSFSPNLFQTGAYITLSVVLVRLLMTRN
                     VPRLFLLLSILIPLLSLSLLGNYRGGILSNEERTSTANLVESEKELFPEEGLTKDATV
                     LTSRPSSRGSADDFDFLVLEEVAEVRLIDSVFLFGFYTIALILVIVVSRKAILEMGRW
                     SRLIPPSIVLLSIVAVLIGGFMYLRSLPLSEEIVLGTGGPSNQTSFAPSQSVTGLPEE
                     NEEDEPEAILNKSAAYNILRWSTLAGILLLSALLAYFVTVIARKNQTVSSTLKSKDTI
                     LTEVRQSASVPPKFSEDREFIISSYKWLREFYFEEYCQLTPFELIQETEASLKHLLIE
                     LTEIYVPVKYGNLEPEIYQCREFHKLLLGLKELLDRKKGTFDNA"
     gene            complement(11634..12905)
                     /locus_tag="PKL93_07995"
     CDS             complement(11634..12905)
                     /locus_tag="PKL93_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNQ71237.1"
                     /translation="MKRLELEEIRSRKGPLIIATGFSVGWSLFFFNVYSAMFLTLSAV
                     VWIYYYLSKLTLKNLTIDRSVDRERLFPGETLTLVHSLQRVPLISLGLTVIPVIRAER
                     EYAAPRESSISLSSTEDSRVESKVVFGRRGEKDLSRLVVACRDPLGLFSHSATYCTPQ
                     SVLVLPKVMDLETFPLRLRELLPGRESDFRLMEDPVDFKGIKEYERESLNRIHWNASA
                     KLGKLMSKEFGYTAVSKTVLYLDLNLSREVFARDVWERIRIDYEEIAVQLALGLIRLS
                     YKSGGSIRLVSVGEEVLRLSDRGRDWIDFAEALSDTKGVDHGFQLNEIIEDDLEQFDP
                     STTVVLISLYLGEEILPQLLRARSHASRVVVLIIPFSPREPWMKRNVSYQMLPKTIEE
                     LKKRAAFLEEEQIIVRVVGDDQSIQEVLLDL"
     gene            complement(12892..13842)
                     /locus_tag="PKL93_08000"
     CDS             complement(12892..13842)
                     /locus_tag="PKL93_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNQ71238.1"
                     /translation="MVSIGEFGSKIKENISRVIVGKEKTIERVLAVLISGGHVLINDV
                     PGVGKTMLARSLAISLGLEFNRLQCTPDLLPGDVTGISILNLKTNEFNFRKGPIFTQI
                     LLVDEINRTTPRTQSALLEAMAERQVTVDGRTFQMEKPFFVIATQNPVEFEGTFPLPE
                     AQLDRFSISLTMGYPDEKSERKLLKGISTEHPITSLKPVSGQVELDSVLKQVKEVVIE
                     DSVLEYIISIVNETRNHRDIQLGVSPRGSIALMETARALAGIRGRRFVIPDDVKETAS
                     DILSHRIIIKPEARLMRRTNRDVINEIVESLPIPINNEKT"
     gene            complement(13992..15005)
                     /locus_tag="PKL93_08005"
     CDS             complement(13992..15005)
                     /locus_tag="PKL93_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ71239.1"
                     /translation="MKKLTIVGAILFVYVLTLGQGNLIIYGWSDYIPSEVIEAFSQEY
                     GVNVTYDSYDSNETMFAKIKAGARGYDLAMPSADYTSIMIKEDMLIPIDKSLIPNLVN
                     IDPSVIEQMYYDPENTYSIPYMVGTTGIAVNTKYVESYPRSWKIFEQPQFKGMMSLLD
                     DMREVFGAALKFLGYSVNTTNTDELQAAKELILTWKENIAKFDSELFAKGVLSGEFWV
                     VHGYGENIFYEATEEELKDIDFFIPVEGSTLWIDSFVILKGAKNIDNAHLFINYILRP
                     DVAAKISDYLLLPSPNVPARELMEEEPVYSAKEMENTELIKDLGEDLRIYNSLWNEIR
                     FGL"
     gene            complement(15021..15809)
                     /locus_tag="PKL93_08010"
     CDS             complement(15021..15809)
                     /locus_tag="PKL93_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ71240.1"
                     /translation="METKKVIGKRRFSMTIATLAFVFLYLPIIALVLLSFNSSRQGVS
                     WTGFTLKWYSELFRQADVWRAFLNTVIIAFSSSLVATAIGTFSAMALYWYQSRLKGYL
                     GILVYTPLIIPDILMGVSMLIFFVAIGIPLGLFTIFIAHVTFCVSYVTVTVMTRLEDF
                     DYSVVEAARDLGAKTNQVFRKVILPMIMPGIMAGFLLSLTLSIDDFVITFFVAGPGST
                     TLPLKIFSMIKFGVSPVINALSTLLLVGTLIISFFGSRFRKLIF"
     gene            complement(15809..16693)
                     /locus_tag="PKL93_08015"
     CDS             complement(15809..16693)
                     /locus_tag="PKL93_08015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71241.1"
                     /translation="MKSKRNLTGFLISMPGVLWLTVFFLIPYLIIILYSFLTPGIYGG
                     VELPFTLAAYTRMLGSAGYWQILWKTVWISLVSTVICLGIGLPTAYFIATSKRSNILL
                     TLVIVPFWTNFLVRIFGWMVILGRNGVINSFLQLIGFDRPLTLMYTPGSVILVISYMY
                     LPYMILPLYSAIEKFDFSLVEAAMDLGAKRTEAIMKVLIPSIRGGIIAGIILVFIPAL
                     GSYAIPDLVGGTDGAMLGNLIAKQLTAARNWPSSSAISMIFLLISTLGLLVYMRINRV
                     QTKRRLTVRESYMNEEEI"
     gene            complement(16683..19064)
                     /gene="potA"
                     /locus_tag="PKL93_08020"
     CDS             complement(16683..19064)
                     /gene="potA"
                     /locus_tag="PKL93_08020"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HNQ71242.1"
                     /translation="MNTLAVAIAIVVLIVSLKLFKDISLSLLFSVISLGIVLLIPPAD
                     YFNAFAKEVSDWEFWKVIITVFSIYLMGETMDKSGNSSRFVRAVESLFPEPRVSIALM
                     PAIIGLLPMPGGAMFSAPMVKDLAKSDPTISNEDGLVFNYWFRHAMEFFWPLYPALVI
                     ASGISRIRLNTLVLWLMPIGVVALVTGYLLMIRKPIKIRYSRSALKELLISAWPIIAV
                     IILVILNQPGWLSVLGTSLVYLLLNKNKKKVLLAALKYKTFILLLTVFFYKDLVEIAE
                     VPQSMGSELIAWSIPPLTLVILLPFLMGFMTGVTQAGVGLSLPLILSMGNGYGTLATT
                     ILAYTFAVVGVLVSPVHLCLVLTSEYFKVEYPRIIKRISVVTLLSVAAGIVVFVLLLS
                     QLELTQLKTQKAILLLALTMTGGENELKEYSVVLSNVTKIFDDSFTAVDNVSLTIEQG
                     EFFSLLGPSGCGKTTILRMIAGFEDPTFGNITLNGKNIIGTPPNEREVNTVFQNYALF
                     PHLNVFENIAFSLRLRNEPEDQVRRKVKEMIDLTRLNGHVEKMPSQLSGGQKQRVAIA
                     RALVGRPTVLLLDEPLAALDAKLRQHMLVELDTIHDEVGITFIYVTHDQSEAMSISDH
                     VAVMNEGEIIQLGTPFEVYESPVNAFVANFIGETNFFTGNVVKVEDEYFLLDTEKFGE
                     IWFYKDMEVLEGQEIQVSLRPEKVKVTRDMPVAPEGVKLNVLKGVIDETIYLGNQTKY
                     TVAIGQSYLKAFKQHVRYLFDEVIITWKDDVYVWWFADDSYVLRETGGVPVEE"
     gene            19132..20067
                     /locus_tag="PKL93_08025"
     CDS             19132..20067
                     /locus_tag="PKL93_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNQ71243.1"
                     /translation="MEKEIFAKRYSDHPEIIERLETSKGSKIFLKSNYPGEYPENGSI
                     PLYLYEGGRKGTVLFFHGRGEKNLDYLRWFPENLAKHGYSGAMMILPFHFERTPAGHK
                     SGELFLDPRTDVLRERFENAVVDGLTTLNYLKWECPPPYFLMGYSFGGFIAVITAALE
                     PSISGLSLVVTGGNFYHITWKSFVTGVMRVKYEEDGSCNREKCRKLHEVDYREFLNSL
                     QKPEIEIGSAPISCFEYDPLTYAKFVRCPTLLTGALFDIFIPRPSTRELAEMLPDATL
                     RWMPSGHLTSILFKKTILKGSIDFFERKCKGKSVL"
BASE COUNT         5315 a         4754 c         4745 g         5441 t
ORIGIN      
        1 cgccagcatc acttcctcgc gccagcgagc ctcacttcct gccggaggca gcatcgcttc
       61 tggtcttgat gccaccaacc cccagaccac ggacttttct cgcttcttat ttcgtaactg
      121 ttgtgttata aatgaaatag tacgtaatcg aaatcatagg agtgaagatg aaacgagtca
      181 atattctgaa tgaaaacatg atgagtgccc tttttaaact cgcctggccg gccgttctta
      241 ccatgctctt ccagactgtt tacaacatgg tcgacgcata ctggctcggg aagctgggaa
      301 agattgagat ctccgcaccc acaattgcgt ggcctgcaat cttcttgttg atctctattg
      361 gcggaggatt agcggtggcc ggacttgcgc tagtctctca aaatctcgga gcaggaagaa
      421 aggaagaggc cacacatgta gccgggcagg ttatttccac aagcttcgcc gtcgctgttg
      481 ttctcggtct tctcggtgcg atcttctccg aagccgtttt gaggcttctc agtattccgc
      541 cagagttgct ttcagttacc aatatttata tgagaacgat ctttctggga gctccattca
      601 ccttcacgat gttcactttc aactcactct tcaccgctat tggtgataca aagacaccga
      661 tgtacttgat gggtttctct gtaacggtga atgcacttat cgatcctctc ttaatattcg
      721 gtataggctt tcccagactg gaagtcttcg gtgccgcgct tgcaactgta atatcaagag
      781 gagttgtagt gataatcgcg acggtcatat tattcaaagg aaagagagga ttcaaaatca
      841 atttgaggga tcttaggcca aagtggaaga ctcttgtccg cgtcttgaga ataggcctcc
      901 catcgtcagc cggacagtcg atcacggctc ttgcgttcct aataattacc tcgatggttg
      961 ccgggttcgg ttcagttgca accgctgccc tgggcgtggg aaacaggatt acttcgcttg
     1021 caacgatgtt ttctttcggc ctttcccagg caacgtcgtc gatggttggg cagtatctag
     1081 gtgctggaag aaagagtgac gcttattcag ttgtatggaa ggcgaccgga ataaacgtgt
     1141 tgattgttgg agtgatcagc accggcacgt tcttcttcgg gaaggaagtg acggcgtttt
     1201 tcatcaacga cccgctcgta ttgattgaag gagagaagta tttcagaata gtctcctttt
     1261 cgataccgat cttcgcaagt tacaacatct tcgacatggc tttaagaggc tcgggccata
     1321 cagttcagtc catgattctc aatatcacta gactatgggg gatacgcctt cctctgatat
     1381 acttcttcgg tatgtccatg ggaatcacag gaatttggta cgcgatgttc gtaagcaatc
     1441 tggtgatttc ctctgcagcg gccatagtta ttttcatgaa gcgttggctg aacccggtta
     1501 tttaggatag aacatgtcaa gtccaatgtg aggtaattac taccgtttac agacgatgtc
     1561 ttcgatattt tacctaaatt ctcttcattg tttcacttcg taatattttc ttattgtcat
     1621 tagtggtagt attcgtacaa catgcaaatg ctcgaatttc ttcgttctgg ctagttaaat
     1681 atacgttcgg gggaataatt agcttagaac actgatttct gtacttgctt agatttgtga
     1741 gaatgtgcat cagattatgg gttagtctca ttcacttgta accacttcag gaggaggtag
     1801 aagtatgaga aaactctttg taattctttt agttgtcttt ctttcagtat cagcacttgc
     1861 ttcaatcaag gtaggtgcga ttcttccgat gaccggtgga gtcgccgcgt ttggccagat
     1921 gatctggcag ggagtcgaac ttgccaacga gcttttcccg gaggttcttg gggagaaaat
     1981 tgaaatcgtc ttactcgaca acaactccga caaggttcag gccgttaaca ccgcccgtcg
     2041 tgcgatcgag caggaagggg ttgttgctat catcgggcag gtaataagct caaacacgat
     2101 tgccggagga acggttgcag aagaaaacgg cgttgcaatg gtttctccca gctcaactaa
     2161 tcccctcgta actcaggaca agaagtatgt ttcccgtgtc tgtttcagcg atcccttcca
     2221 gggggttgcg gcagcgatgc ttgcattcaa taacatgggt gcggaaaacg ttgctgtctt
     2281 cgtagacgtt gagcaggatt atgccgtcgg atttgcaaat tacttcaagg aaacggctaa
     2341 cgagcttggt ggaagcgtat tctacgagta ctacaaatct ggagaccagg acttcacagc
     2401 ccaggtttcc gatgccatct ccaagggtgc cggtgccttc ttcattccgg gttactatca
     2461 ggaaatcgcg ctaatagcca tccaggccag acaacttggt ttctacgagc cgataatagc
     2521 aggtgacggg gcggccgtgc cggaaactat tgaaatcggt ggagatgccg taaacggact
     2581 gtactttaca actcactacg atgcaggaag tccagcaatt acagagaatg cgaagctttt
     2641 cgtagaagcc tacaccgcca agtacggtga agcgcccgga acattcaccg ccctcgggtt
     2701 cgacacatat ctggtcgtca gggacgcaat agaacgtgcg gggagcacag atagagaagc
     2761 catagccagg gccgttcgac agacgaagta ttatcctgcc gtgacgggaa taatcactat
     2821 cgatgaaaac ggtgacgcga taaagtccgt tgcgattgta aagattgaaa acggtaagtt
     2881 cgtgtacgat acaacgataa atccgtagta aagaaggtgg ggctaaaagc cccaccctcc
     2941 cctttctctt ttcaatcggc cggaggtgta agcttggatc ctaagactct tctacagaac
     3001 tttatcaacg gtcttagtct cggttccctt tatgccctga tagcgatagg ctatacaatg
     3061 gtttacggga ttttaaggct gatcaatttt gctcatggcg acatcttcat gatggccgtc
     3121 tattttgctc tgttctttgt gactcttgcg cagcttccat ggtatcttgc ggccattctt
     3181 gccgtagctt gcgctgctct tcttggattt acggttgaca ggattgccta caaacctatc
     3241 aggaatgctc ctagaatatc ggcgctgata acggctatcg gtgtttcttt ctttcttgaa
     3301 agtcttgcgg tagtcgtatt ctccggtatc ccgagatcat tccgtactat cttcccccaa
     3361 tctctcaatg aaatgattat catcggcggt gagatggagg tcaaatatgg gagagaggtg
     3421 atagtcggcg ggataagatt tccggtaatc tctctcataa cacttatcgt tgctgcaatt
     3481 gcgctcgtct ttttgtggtg gttcatcttc aaaacaaagg tgggaatggc tatgagggcc
     3541 gtttccctgg atattcagac aacatcgctt atgggagtca atgtcgacag ggtaatcgga
     3601 atgacatttg cactcggctc ggcgcttgca gggattggtg gaatactctg ggcaataaga
     3661 tatccccagg tctggcccta tatgggattt atacccggaa tgaaggcctt tgtcgccgcc
     3721 gtcttcggag gaataggttc agtgccggga gcggttctcg gaggtctgat acttggaata
     3781 accgaagtca tgatggtagg aataatgccc ggggcggcag gttacagaga tgccttcgcc
     3841 ttcttcatcc tgataatcgt cctttcaatc aagccatccg gtctgcttgg aaggcctgaa
     3901 atagtaaagg tgtgattgaa tgaagaagcg gaccaaaatg atactcactt tgatagcatt
     3961 accctccata gcgcttcttc tttttctttc gcaggaatta ctgaacagct attattcgag
     4021 aataataacg cttattggta tatatggaat catggcggta agccttacat tggttaatgg
     4081 aatatcggga gtcttctcgc tgggacatgc agggtttatt gcggttggag catacacttc
     4141 cgcgctcctg actctgtcgc cggagcaaaa ggaaatgacc tttctcattg agaaactggt
     4201 gtggccgctg aactctattc agattccctt cttctgggca acgataattg ccggtctcgt
     4261 cgcggccgta tttgctttcc tgattggctg gccgtcgttg aggcttacag gtgactactt
     4321 tgcaattgcg acgctcggtt tttccgaaat aatcaggatc tttgcgctta atctcaattc
     4381 aataacaaac ggggcgctgg gcctaaaggc gcttcccgat cacacgaatg tctggtgggc
     4441 atggggctgg ttgcttgtta ccgtagccac tgtcctgagt cttgcttaca gttccttcgg
     4501 aagggccttg agggctatac gagaagacag agtcgcggct caagcgatgg ggataaatgt
     4561 cttcaagcat cagttgacag cctttgttct tggcgggttt tttgcgggga tttccgggtc
     4621 tttgtacgga cactggctca gcacaatcga tcctagaaca aacacgtttg gcatccttct
     4681 caccttcaac gttctgataa tgatagttct cggcgggctg ggaagcataa caggagctat
     4741 aatcggcggt gcgctctttg ctttccttag cgagtggcta cgcttcctcg agggccccat
     4801 gaatatcctg ggtttcaaga ttatgggcat aagcggtatg agaatgctcg tcttctcggg
     4861 actcttcgtg ataattatga tcttctggcc gaggggactg atgggaagaa gtgagttctc
     4921 ctgggatgga ctcgtttctg ttttcaagag gagatacaag agatgagtct gctcacactt
     4981 gagaatgtaa caatgaagtt cggcggactc accgcagtga aggatgtaac gcttcgcgta
     5041 gaagaaggcg agatctttgg tttgatcggc ccgaacggtg ccggaaagac aacgatcttc
     5101 aatatgataa cgggacacta caaacctaca gatggtcgta tattgttcag ggaaaatgag
     5161 attacggcac tccaaccgga caagataaca agaactggga ttgcaaggac ttttcagaac
     5221 atccgtctgt tcagagatct taccgtttta gagaacgtaa tggtttctca acaccatacg
     5281 attgcaagca acggaaaggc cgtaagctgg tttttcaaaa gcgttaccag actgggttat
     5341 tccgagaaag aacgggagat gagaaacaag gctcttgaac tgctgagtgt tctcggtctc
     5401 gaaatgctgg cgaatgaaaa gtccagcgcg ttatcttatg gttcgcaaag gctactggag
     5461 attgcaaggg caatggcgac gggggcgact cttctccttc tcgacgaacc ggctgccggg
     5521 atgaatgcct ctgaaacggc aggtcttgta gatactatca ggagaattcg cgatgatttc
     5581 ggccttacag ttctactcat agagcatgat atgaaactgg taatggggct ttgtgaaagg
     5641 ataatggttc tggatcacgg aaggaccatc agtgaagggg accccgcctt tgttcagaag
     5701 gacgtgaggg ttatcgaagc ctatctgggt agggagtggg ttacggttgg caaatgaaat
     5761 tctcagcgtt gatgagctcc aggtttcgta cggtgtcatc aaggcagtaa aaggcataac
     5821 gctctccgtt gaggagggca gtattgtaac tataataggc tctaacggcg cggggaagag
     5881 caccacgctt ggtgccgtaa gtggattgat aaggccctcc ggtggaaaag tctctttcag
     5941 agggaacaat atcaacagac tgggagccgc cagaacggca aaactgggga tttcgctcgt
     6001 acctgaagga aggaggatat tctccaatct cacggttaga gagaatcttc ttatgggagc
     6061 atacaacagg aaggacaaag aagagataga aggcaacttc gaagcggtct tctcgctttt
     6121 ccctgttcta aaagagagat tgaaacagtc cggaggaacg ctttcgggag gagaacagca
     6181 gatgctggct attggtagaa gccttatggc gaatcccgat ctaataatga tggatgaacc
     6241 ttcgctcggt ctagcaccca ttgtcgttga agaggttttt caggcaatcc agacactcaa
     6301 ttcgcgggga gttaccatac tgcttgtcga acagaatgcg gccaaggccc ttgaaatatc
     6361 gaaatatgct tatgtgctgg aaacaggaaa gattacactt caaggttcag caaaggaact
     6421 acttgaaagt gaagaggtta gaaaagtgta cctgggaatt tgagagaatc acccttaggc
     6481 ccctttttgc ggggcccttt tcttttttct aatgtgtgct cgcagccccg gttcttctcc
     6541 cagcaaaatc ctctgaatat tctcgaggtt tctagctatc atgattactg caatgggtat
     6601 cagcagaaga gatattcccc atctgtaccc aaagattctg aaaaggaaga tcattactga
     6661 agagactgca ataatgcccg tagccgcgta gtccgtaatc gcgataaaag cgagaagtat
     6721 gaccatacag atcagggcga cctggtaatc aagacctata aggccaccgg ccagtgttgc
     6781 ggttcccttc ccacccttga acctcagaaa gacgggatac atatgtccga tcgcggcaaa
     6841 actggccgca atgaaaagca tctgataggt ccagtgaggg gagacgccct taataatcaa
     6901 gatcgttccc aggcccttca tgaagtcgat agctccggtg aggattccga tcttccatcc
     6961 ttttacggca accatgttgg aagctcccat attaccggtg ccctgttttc ttatgtcgaa
     7021 tccatagaga ctcttcccga tgagaaaagc tgtgggaata gatccaatca gataggccaa
     7081 gagcgcggag aacaagattt ccatttttac gtcacgactt ctttctggaa aaccagggga
     7141 caaatgcgtt tgtttctttc atgtactccc tgaatgccgg gtactgcttg tatttccttt
     7201 ccagataggg cactccagaa acgaatctca acaatatggt tattgttatc gggcttatga
     7261 ttgcccatat tcccccttca attgaaaggg caatgacgaa tataccccac cactgaacgg
     7321 cctcgccgaa ataattgggg tggcgagaat atctccacaa accctttgtc attatctctc
     7381 cgggtttctt agtcttcacg aaatccctta actgcttatc tgctattgct tcgatggcaa
     7441 agccgcaggc ccaaataaga attccgagaa cgtccaaaaa tccaaatgac cttcccgaat
     7501 ggctattaat gagcattact gagtaagaga taatcaccat aaaaaggccc tgcaacatat
     7561 agatctgcag gaaggatcta atcaatacct tctctcccca cttctctctc atctgaacgt
     7621 aacggaaatc ctcaccacgc ccccagtttc ttttaaaaat atgtgtggtc aatctgattc
     7681 cccaaaacgc aatcaagata gtcgtgacta cctgccttaa attgaactct ccatacacga
     7741 agagcgtgaa caaagcaaca acaacgaatc cagcgcccca gcctatatcg acaacggaat
     7801 tgtctttctt taaagtgccg atagcgaaga acagagacat gtaagcaagg accactaaag
     7861 aactgaaaag aatcacataa gtcatctctt cacctcactt gatattgaca tcgggagacc
     7921 aggtgtctct ttcgcaaagc acagatacgc ttacagcgcc gacccccgag ctaataccta
     7981 caacagggga gacttccata atgtactcgg gcttctttcc aagcagtttc atgaggcttt
     8041 cctcatacgc tttcgcggtc ttgtgggcat gagcgtgagt gatattgtaa gatacaacgc
     8101 tgcacttttt gttcagttct tccacaaaac caagtatctt tttcatgttg cctaacttgc
     8161 tgaaggcctt tccgtagagt ttcgaatttc cttccttgtc taccgataca atcggcttaa
     8221 gattcattgc cttaccgacc agccccagaa ctggacttac ccttccacct ttcaccatgt
     8281 acttcaaagt gttgacactc acgaagatcc ttgtgttttt tgacagagac tcgattgttt
     8341 ctgcgatctc atcgtggcta cgaccttcct ctatcattct ggcggccgtg agcgcgagca
     8401 gaccaataga tcccgataca tgccgggaat tgattacgct tatcttcttg ctcttcattc
     8461 gttctgcagc tttggtgctg gcgttccatg taccgctaag tttgtccgag acgtggatag
     8521 ctataataga atcataatgg gttccaagaa agtggtacaa attctggaag gtgttcagtc
     8581 ccggctgagc cgaaaccgga tagtcctccg tcgtgtcgag aagtgtgtag aattggtctg
     8641 ccttcaatgt tattttgtct agatactgac tcttgccgaa gttgagatac aatggcacca
     8701 tctgaatctg gtagtagtca attacctcct tcggtaaatc gcaaacggag tcaacaacaa
     8761 gtgcgatcgg atacttcctc ttttgactca ccctgtactg catctccatg tcatcgactt
     8821 tctgctgagt aactgtgccg aagtccttca gcttgaagaa gagctctgcg ggttcattag
     8881 tgtgaacgtg gattcttgcc ttagtgtccg ttcccgctac cagggcggaa tctccgaact
     8941 gactgatgat cgactttatt tcattgacgt ccagtcgaga accttcgatt atcgcctcgg
     9001 tgcagtatct attcttgatg ttgccttcat ctatatggac gtgttttttc aattcgatat
     9061 cggggatgat ctcgtcttca agattgacgc ctcttccgct cagaaaagtc tttatacctt
     9121 cgatgaaggc tacgaagccc ttcgctccgg catcgactac gccggccttc ttcagcacgg
     9181 tcaatttctc aggtgtttct tcgagagact tgagcgctac ttcgttcgac cgttcgacca
     9241 tctcgaaaaa cgatctttcc gagtcttcta tggcgaccaa agcctcggcc caatccttca
     9301 tcacggtcag catcgtcccc tcgacggggg ttaccatagc atcataggca tatgaaatcg
     9361 cctcatgtgc tgcctttgcg aaggtctttc tatcgatact gtctctgtct ccaatgatgg
     9421 tactcagtcc gtgcatatac tgggcgaata tcgctcccga gtttccacga gctcccatga
     9481 gtgcggcatc ggctatcgat cgcgttgtct tgccaatcga atgagagaca tctgtcagct
     9541 caagaataga tcgcatcgta gaggcgagat tcgtcccggt gtcgccgtcg gggacaggaa
     9601 agacgtttat cctattcagg tcgttctgct ctcttattac ggcagtggca ccagaagaaa
     9661 aggcattgaa gaacatcgtt ccgtcaagca gattcaaaaa tttggagctc atcgttacct
     9721 cccacgaatt agttcgatta ttgatatgtt agcacaatga ccggggatta gtcagatggt
     9781 cacgatacat aaaaaaatcg cccgctgaaa gcgggcgatc gggttcctta tatggacaat
     9841 cgattacttc gcaattctaa cttttgctgc ctgaggaccc tttggaccgt cttcgacttc
     9901 gaattcgact ctctgtcctt cgtcaagggt cttgaaaccg tccatctcaa tagctgagaa
     9961 gtgaacgaaa acgtccccac cgtcatcctt agtaatgaat ccataacctt ttgttccgtt
    10021 gaaccacttt actgttcctg tcatacttaa atgttcctcc ttaaaactac cttgcaccac
    10081 ttgcaaagcg atagtgtcct ttggacagaa tgattataac acacacaact gaataaaatg
    10141 caagccgatt ccaaatgaat ttgattattt ctgaaatgca agctgttcaa catgcgaaaa
    10201 gccgcacaca tagtaattgc tatgcattat caaaagttcc tttttttctg tccagaagtt
    10261 ccttcaggcc cagtaaaagc ttgtgaaact ctctgcactg atagatttcc ggttctagat
    10321 tcccatattt gactggaaca tagatttccg taagttcaat tagaagatgc ttcagcgaag
    10381 cctccgtctc ctgaatcagt tcgaagggag ttagctggca atactcttca aagtagaact
    10441 ctcttaacca cttgtagctt gagataatga attctctgtc ctcactgaat ttgggaggta
    10501 cagacgcgct ttgacgaacc tccgtcaaga tcgtgtcttt ggatttaaga gttgaagata
    10561 cagtctggtt ctttctagca atgactgtca caaaatacgc aagaagggca gaaagaagga
    10621 gaattccagc tagagtcgac catctgagaa tattgtaagc cgccgatttg ttaaggatgg
    10681 cttcgggttc atcttcttcg ttttcttcag gcagtcctgt aaccgattgg gaaggcgcaa
    10741 aggacgtctg attggaggga ccgcccgtcc ctaatacgat ctcttcggaa agagggagag
    10801 atcttaaata catgaatcct ccaatcagta ctgccactat cgaaagaaga acaatagacg
    10861 gagggataag acgactccat cgccccattt ccaggatggc cttcctggat acaacaatta
    10921 caaggatcaa agctattgtg tagaatccaa agagaaagac agagtctata agccgtacct
    10981 cggcgacttc ttcaaggaca agaaagtcga aatcatctgc agaacctctt gaagagggtc
    11041 gagatgtcaa taccgtagcg tctttggtga gaccctcttc tgggaataat tccttttcgc
    11101 tttctactag gttggccgta gaggttcttt cttcatttga taagatgcct cctctgtagt
    11161 ttcccaaaag gctcagagaa agcaggggga tcaagattga tagcaaaagg aagagtctcg
    11221 gaacattgcg tgtcatgaga agtctgacaa ggactacgga aagtgtaatg taagctccgg
    11281 tttgaaagag attgggacta aaagaaagcg aaactcctac catgaagaga ccgggaactg
    11341 caagacggac acttctgtcc atcgagagta ttactcccac aatagatccc agtgcagaaa
    11401 gcgattgtac gacatccgtt ctcaagacgc ctataagcag tatcgttata gtaaagagta
    11461 agaccgggag aatgaagtag atactgcagc cggccttagt tcttccaaag tagaagataa
    11521 agcctaccat tcctgcgagg attaatgcta aaacagctcc accggaaaag aacagctgta
    11581 aataactgac agtgaatacg acggtcgagg cggctataga ggttttgtct attttagaga
    11641 tctagaagca cctcctgaat cgactggtcg tctccgacca ctcgtacgat tatctgctct
    11701 tcttcgagga aggccgcgcg tttcttaagc tcctcgatcg ttttcggaag catttgataa
    11761 gatacgtttc tcttcatcca gggttctctg ggactgaacg gtattataag gactacgact
    11821 ctgctggcgt gagatcttgc cctcaagagt tggggcagaa tctcctcgcc gagatatagc
    11881 gatattagga caacagttgt cgagggatcg aactgttcaa gatcatcttc aattatttcg
    11941 ttcagctgaa atccatgatc gacccccttt gtatctgaaa gagcttcggc gaagtctatc
    12001 cagtctctcc ccctatcgct caatctcaat acctcttccc ccacgcttac cagtcgtata
    12061 cttccaccag atttgtagga aagccttatt agacctaggg caagctgaac ggctatctct
    12121 tcgtaatcta tccttatcct ttcccagaca tcacgggcaa agacctctct cgaaagattc
    12181 agatccagat agagaactgt tttgctcacg gcagtgtatc caaattcctt actcattagc
    12241 tttccgagtt tggctgaggc gttccagtgt atcctgttca gagattctct ctcgtattct
    12301 tttatcccct tgaagtcaac ggggtcttcc atcagtctga agtcgctctc tcttccggga
    12361 aggagttctc ttaaccttag aggaaaggtt tcaagatcca tcactttggg aagtacaagt
    12421 accgattgag gcgtacaata cgtggcgctg tgagagaaaa gtcccagcgg atctctgcaa
    12481 gcaacgacca gtctcgaaag atccttctcg cctcgtcttc cgaaaaccac cttcgattcg
    12541 actctgctgt cctcggtaga ggaaagggag atcgaactct ctcttggtgc tgcgtactct
    12601 ctctcggctc taatgaccgg gattacagtg agaccgagag aaatgagggg taccctctga
    12661 agactgtgaa cgagagtaag ggtttcgccc ggaaagagcc tttctctatc aacggagcga
    12721 tctattgtaa gatttttcaa ggtgagttta gaaagatagt agtagatcca tacaaccgca
    12781 gaaagggtca aaaacattgc cgaataaaca ttgaaaaaga aaagggacca accgacagag
    12841 aagcccgtcg caattatgag cggtcctttt cgagatctta tctcctccag ctcaagtctt
    12901 ttcattattg atcggtattg ggagagattc gactatttcg tttattacat ccctgttagt
    12961 ccttctcata agccgggctt caggcttgat tattattctg tgggaaagaa tatcgcttgc
    13021 cgtttccttg acatcgtcgg gaatgacgaa tcttctgccc ctgatacctg caagggccct
    13081 ggccgtttcc ataagggcga tcgatcccct tggacttacg ccaagctgta tgtcccggtg
    13141 gtttctggtc tcatttacga tagagattat gtattcgaga acggagtctt ctattactac
    13201 ttccttgacc tgctttagaa cggaatctag ttcgacttga ccggatactg gcttaagaga
    13261 agttatcgga tgttcggtgg agattccctt tagaagtttt ctttcgcttt tctcatcggg
    13321 gtatcccatc gttagggaga tggagaatct atcaagctgc gcctcgggaa gcggaaaggt
    13381 tccttcaaac tctaccgggt tttgagtggc aattacgaag aagggtttct ccatctggaa
    13441 agtcctgccg tctacggtga cctgtctctc ggccatcgcc tcgagaagag cggactgcgt
    13501 tcttggggtc gttctgttta tttcatctac gagaagaatc tgggtgaaga ttggaccctt
    13561 tctgaagttg aactcgtttg tcttcaggtt gagaatactt atgccggtta catcaccagg
    13621 cagaaggtcc ggagtgcact gcaatctgtt aaattccagc ccgagagaaa tcgccaggct
    13681 tctcgccagc atcgtcttac ccacgcccgg cacgtcgttt atcaagacgt gtccaccgct
    13741 tatcagaacg gccagcaccc tctctatagt cttttccttt ccaacgatta ccctggaaat
    13801 attctccttg atcttgcttc cgaattctcc aatagaaacc atctttcacc tcttaggaag
    13861 tctgcagcct gtagaagtgt aacacatgca aacaaatgct cgcaaaccct ttccaacttt
    13921 caaagaaaaa cgcatctcct ctagaagacg cgttttttga acgggctctg attgccttgt
    13981 ttggctctat actaaaggcc gaaccgaatc tcgttccaga gagagttgta gattctaaga
    14041 tcctctccaa ggtcctttat cagttctgta ttttccatct ctttggcaga gtagacaggt
    14101 tcttcttcca taagttctct tgccggcaca tttggtgatg gtaagagaag ataatcggaa
    14161 atctttgctg caacatccgg cctcaatatg tagttaatga acaagtgggc gttatcgata
    14221 tttttggccc ccttcaggat gacaaaactg tcgatccaca aagtgctgcc ttcaaccgga
    14281 ataaagaaat cgatgtcctt aagctcctct tcggttgcct cgtagaagat gttttctccg
    14341 tagccgtgca caacccagaa ctcccctgat aacacgcctt tagcgaagag ttcgctgtcg
    14401 aatttcgcaa tgttttcctt ccaggttagt attagttcct ttgccgcctg gagctcatcc
    14461 gtgttcgttg tgttgacgga gtatccaagg aacttgagtg ccgctccgaa gacctctctc
    14521 atgtcgtcca gcaacgacat cattcccttg aactggggct gttcaaagat cttccatgac
    14581 ctaggatagc tctctacgta tttcgtattg acagcaattc ccgtagttcc taccatgtag
    14641 ggaatgctgt atgtgttttc gggatcgtag tacatctgct caattacaga gggatcgata
    14701 ttgaccaaat tgggtatcag agatttgtct atcggaatga gcatatcttc cttgatcatt
    14761 atgctagtgt aatctgcaga tggcattgca agatcgtatc ctcgtgctcc ggccttgatc
    14821 ttggcgaaca ttgtctcgtt cgaatcgtag ctgtcgtaag tcacatttac gccatactcc
    14881 tgcgaaaagg cttcaataac ctcactggga atgtagtccg accaaccgta gattatgaga
    14941 tttccctgac ctagtgtgag cacgtaaacg aaaagtatgg cacctacgat tgtcagtttc
    15001 ttcatcaatt gttccacctc ctaaaagatt agcttcctga atctgcttcc aaagaaagag
    15061 atgattagag tcccaacgag gagcagagtc gaaagcgcat ttattaccgg ggaaaccccg
    15121 aactttatca ttgaaaagat ctttaaaggc aaagtagtag atcccggacc ggcaacaaaa
    15181 aaggtaatga caaaatcgtc tatcgacaat gtcagactca gcagaaagcc tgccattatc
    15241 cctggcatta tcattggaag aatcactttt ctgaagacct gattggtctt cgctcccaga
    15301 tctcttgccg cctcgaccac agaatagtcg aagtcctcca gtctggtcat caccgttacc
    15361 gtgacatatg agacacaaaa ggttacgtgc gctatgaaaa tcgtgaaaag acctaatgga
    15421 atgcctattg caacgaagaa tatcagcatc gatacaccca ttaaaatgtc cgggataatg
    15481 aggggcgtat aaaccagtat gccgaggtag cctttcaacc ttgactgata ccagtagagg
    15541 gccattgccg aaaaggttcc tatagctgtc gccacaaggg acgacgaaaa agctatgatg
    15601 actgtgttga gaaaggctct ccagacatcg gcctgtctga acagctcaga ataccacttc
    15661 aaagtgaagc ccgtccacga aacaccctgt ctggagctgt tgaatgacag tagaactaga
    15721 gcgataatcg gcaggtatag aaagacgaag gccagagtgg ctatggtcat agagaatctg
    15781 cgcttcccga ttaccttctt cgtctccatc aaatctcctc ctcgttcata taactttcgc
    15841 gaacggtaag ccgtctcttg gtctggactc tgtttattct catataaacg aggagaccca
    15901 gagtggagat caacagaaat atcatcgaaa tcgcagaaga ggagggccag ttccttgccg
    15961 ccgtcagctg tttggcaatt aggtttccca acatcgctcc gtcggtccca cccacaaggt
    16021 cggggatagc gtaagaaccg agggcgggta tgaataccag gattattccc gctattatcc
    16081 cccctctgat cgaagggata agaaccttca ttatcgcctc ggttctcttt gcacctagat
    16141 ccatggcggc ttctacgaga ctgaagtcga acttttctat cgcgctgtaa agcggtagta
    16201 tcatgtacgg aagatacata taggatatga caagtattac tgaacccggc gtatacatga
    16261 gcgtcaaggg tctatcgaaa ccgatcagct gtaaaaacga gttaataacc ccatttcttc
    16321 caagaataac catccaccca aatatccgca caaggaaatt cgtccagaat gggacgatta
    16381 caagggttaa aaggatattg cttctcttcg aggtagctat gaaataggca gttggcagtc
    16441 caattcccag acatatgact gttgagacaa gagatatcca cacggtcttc cagagtatct
    16501 gccagtatcc agcactgccg agcattctgg tgtatgcggc gagtgtgaag ggaagttcta
    16561 ctcctccata tattcccgga gtgagaaagc tgtacagtat gattatcaaa tatggaatca
    16621 aaaagaaaac ggtaagccac aggaccccgg gcatggagat cagaaatccg gtcagattcc
    16681 gcttactctt caacaggaac accgcctgtc tctcttaata cataactgtc atcggcaaac
    16741 caccagacat agacatcgtc tttccacgtg atgattactt catcgaacaa gtatctaacg
    16801 tgttgcttga acgcctttag atatgattgg ccaatagcta ccgtgtactt cgtctgattc
    16861 ccaaggtaga tagtctcgtc gatgacgccc ttcagcacat tcaacttgac accttcggga
    16921 gcgaccggca tatccctggt aaccttaacc ttctcaggtc tcaatgatac ctgaatttcc
    16981 tgaccctcta gcacttccat atctttgtag aaccagatct ctccaaattt ctcggtgtcc
    17041 agcagaaagt attcgtcttc tactttcacg acattccctg tgaagaagtt agtttctcct
    17101 atgaagtttg ctacgaaggc gtttaccgga ctctcgtaga cttcgaaggg tgttccaagc
    17161 tgtatgatct ccccttcatt catgacggcc acatgatcgg aaatcgacat agcttcactt
    17221 tgatcgtgag ttacataaat aaatgttatg cccacttcat cgtgaatcgt atctagctcc
    17281 accagcatgt gttgccttag ttttgcgtcg agagccgcaa ggggttcatc gagaagcaac
    17341 actgtcggtc tccccacaag ggcccttgcg attgccactc gctgtttctg gcctccggag
    17401 agctgggaag gcatcttctc gacatggcca ttgagacgcg taagatctat catttccttg
    17461 accttacgcc tgacctgatc ctctggctca tttctcagtc taaggctgaa tgcaatgttc
    17521 tcgaatacat ttagatgagg aaagagtgcg tagttctgaa aaacagtatt gacttccctt
    17581 tcgtttgggg gtgtaccgat aatgttcttt ccgttgagag ttatgtttcc gaaggtgggg
    17641 tcctcaaatc cggcgatcat tcttagtatc gtagtctttc cacaaccgga gggacccaga
    17701 agagagaaga actcgccctg ttctatcgta agagaaacgt tgtccacagc agtgaaagaa
    17761 tcgtcgaaga tcttggtcac gttcgaaagg acaacggagt actctttcaa ttcattctca
    17821 cctccagtca tcgttaaagc aagtaacagt atagcctttt gggtcttcag ttgtgttaat
    17881 tcaagttgac ttaaaagtag cacaaagacg acaatgccgg ccgcaaccga gagcaaagtg
    17941 accaccgata tcctctttat gattctcgga tactcgactt taaagtattc gctcgtcaaa
    18001 acgagacaga ggtggaccgg cgaaaccaga actccaacaa ctgcgaacgt gtaagcgaga
    18061 atcgttgtag caagtgtacc ataaccgtta cccatactga gaattaacgg aagactgaga
    18121 cctaccccgg cctgagttac ccccgtcatg aatcccatta ggaacggaag aagaattacc
    18181 aaagtcaggg gcggtatcga ccaggcgata agctcgctgc ccatagattg cggaacttct
    18241 gcgatttcaa caaggtcttt gtaaaagaag acagttaaaa gcaagataaa cgtcttgtat
    18301 ttgagagccg ctaatagcac ctttttcttg ttcttattga gaagcaaata gactagcgat
    18361 gtcccgagga cggacagcca gcccggctga ttaagtatca ccagtatgat aactgcgatt
    18421 atcggccagg cgctaatcaa aagctctttt aacgccgagc gactgtatct gatcttgatc
    18481 ggctttctaa tcatcagtaa atatccggta accaaagcga caacgcctat gggcattagc
    18541 caaaggacaa gcgtattgag tcttatcctg gatatgccag atgcaataac cagagcaggg
    18601 tacagaggcc agaagaactc cattgcgtgc ctgaaccagt agttgaagac aaggccgtct
    18661 tcatttgaaa tagtggggtc ggattttgct agatctttaa ccatgggcgc ggagaacatt
    18721 gccccgccag gcatcggaag gagtccgatt atcgcaggca tcaacgcaat cgagactctt
    18781 ggctcgggga aaagactttc aacggctctt acaaatcttg aagaattgcc gctcttgtcc
    18841 atcgtttcac ccatcaagta tatggagaaa acagtgatga ttaccttcca gaattcccag
    18901 tcgctcacct ctttggcaaa agcgttgaaa tagtctgcag gtggaatgag aagaacaata
    18961 cccaaagata tcactgaaaa gaggagtgac aaagagatgt ccttaaagag ttttagagat
    19021 acaatcaaaa cgacgattgc aattgcgacc gctaaagtat tcatccgttc ctcccagaag
    19081 gtacaggtta gcttatcgaa ttattatata cttttataga ggtagatgta aatggaaaag
    19141 gagattttcg caaagcgtta ttccgatcac ccagagatta tcgagagatt ggaaacaagc
    19201 aaaggttcta agatcttcct caagtcgaat tatccaggcg aatatccgga gaatggcagc
    19261 atccctctgt acctttacga agggggcaga aaagggacgg tgctgttttt tcatggaagg
    19321 ggagagaaga atctagatta tctcaggtgg ttcccggaga acctcgcgaa acacggttac
    19381 tccggtgcca tgatgatact tccctttcat tttgaaagaa cccccgccgg ccacaaatcg
    19441 ggggagttgt ttctggatcc caggacggat gttctaagag aaaggtttga aaacgccgtt
    19501 gtggatggtt tgactacttt aaactacctc aagtgggagt gccccccacc ctacttcctc
    19561 atgggctaca gcttcggggg ttttatcgcg gtcattaccg cagcgttaga accttccata
    19621 agtggattgt cgcttgttgt caccggaggc aatttctatc acattacctg gaaaagcttt
    19681 gtgaccggag taatgagggt caagtacgaa gaagacggat cctgtaatag agagaaatgc
    19741 cgtaaattgc acgaagtaga ttacagagag ttcttgaact cactacagaa gcctgaaatc
    19801 gaaatcggga gtgctccgat ctcgtgcttc gaatacgacc ccctgactta cgcaaagttt
    19861 gtcaggtgtc ccactctctt aactggagct cttttcgata ttttcattcc tagaccttct
    19921 acgagagaac tcgccgaaat gcttccagat gctactcttc gctggatgcc ttccggccat
    19981 cttacctcca tattgttcaa aaagacgata ttgaagggtt ctatcgattt cttcgaaaga
    20041 aaatgtaaag ggaagagtgt tctctaaaac gcgcgtatcc tccgcggtcg cgcacacgcg
    20101 cgaggatcga ggatagaggc cagtggtcag agcagaaaat tagttgcaag atgcagtgtg
    20161 ccaagaaggc gaagaaggaa acttcaagcc atgcttaatc gaagatgagg gagggcccct
    20221 cgaagccgcc atgcaggtgg aggcttcaaa ccacc
//
LOCUS       DAOQYI010000030        19103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_3254_length_19103_cov_68.722491, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000030 DAOQYI010000000
VERSION     DAOQYI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 19103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19103
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_19103_cov_68.722491"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /gene="priA"
                     /locus_tag="PKL93_08030"
     CDS             <1..836
                     /gene="priA"
                     /locus_tag="PKL93_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNQ71244.1"
                     /translation="HCSVSMTYHKADNSLKCHYCGYKEPIPKTCPSCGSMTLSARGFG
                     TERVEHDLQKYFPSVKIMRMDRETIDNPIAYEKALLEISKKESQIIVGTKMITKGLDF
                     PDVEMVLVVDADRLMSFPSYDSPETAFQHISQVSGRSGRAGIGKAFIQSFNPKNRIIK
                     AAFERDYEAFYKDEIALRKELNNPPFAKIAEIICYGDTEDKSGELAERILSEVKAERL
                     DTIEVFGPLTPLLSKLKNSYRMKITIKLPSDSDCGFLEKIQKRHPADVQIIINGIGGI
                     I"
     gene            complement(839..2038)
                     /locus_tag="PKL93_08035"
     CDS             complement(839..2038)
                     /locus_tag="PKL93_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ71245.1"
                     /translation="MSNDNKSKLLLRTDNLVKYFPVKAGVFRRVVAQVKAVDDVSFSV
                     YEKETLGLVGESGCGKTTAGMTVLRLYEPTSGRIIVGDEDTTHFFMPSLKARKYIKQT
                     YIEKFSDLKEKSGSVEGAISSIEDGFDKKMAEKYFKEFNEDAGEFIKSLLGNREEKRK
                     AFRRDAQIIFQDPFSSLNPRMRVKNIIGEGARIHGLATGRDVMDKVADIMTKVGLSKD
                     HMSRFPHEFSGGQRQRIGVARALILNPRLIVCDEAVSALDVSIQAQILNLLSDLQKDF
                     GLTYLFIAHDLGVVKHVSKRVAVMYLGKIAELADKKKLFDNPLHPYTVSLMSAIPEAN
                     PEKKKQRILLEGDVPSPINPPTGCRFHPRCPIAKDICSKEEPKLQDIGGGHYVSCFFP
                     GELKRGF"
     gene            complement(2035..3051)
                     /locus_tag="PKL93_08040"
     CDS             complement(2035..3051)
                     /locus_tag="PKL93_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71246.1"
                     /translation="MNERKALLQVEDLRTYFHTEDGIVKAVDGVTFDVFPGETLGIVG
                     ESGCGKSVTSLSIMRLLDEKGEIAGGKIIFDGEDVMAIPESKMMKIRGNEMAMIFQEP
                     MTALNPVFTIGFQIMEAILLHQDVDEKKARQMAIDMLKKVGIPEPEKRIDEYPHELSG
                     GMRQRAMIAMALSCNPKLLFADEPTTALDVTIQAQILELMKSLQDQYGMALVMITHDL
                     GVIAEMAQRVVVMYAGKVVEYADVHTLFKNPRHPYTWGLMNAIPRLDEDKEVLYNIPG
                     VVPDPLDFPDGCRFNTRCPLATEKCRKEEPPLVEIENDHTAACWHIDKLIETIKVARA
                     GESA"
     gene            complement(3065..3946)
                     /locus_tag="PKL93_08045"
     CDS             complement(3065..3946)
                     /locus_tag="PKL93_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71247.1"
                     /translation="MPAKRKSEFRKIMKKYWTNGTAVLGTCLLIFFIIIAIFAPQIAG
                     VNEMGDNYQIPRASWSSKPIAPSAEHPFGVIGGRDVFYGVIWGTRTAFRLGLIITSMA
                     ALIGVIVGSISAYFGGWVDEILMRITDIFLSIPFLVAAMVMTTVLGKGLDKVIIALII
                     FGWMSTARLIRGNILQAREEQYVLAAKALGQKDWKIIIKHILPNTIFPVVVQMSMRIG
                     SYVITAAGLSFLGVGAEPGYADWGTILSYSRNWMTMLDQSWFAIVFPGTAMVLFVLAW
                     NLVGDALRDIFDPRMRS"
     gene            complement(3961..4995)
                     /locus_tag="PKL93_08050"
     CDS             complement(3961..4995)
                     /locus_tag="PKL93_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71248.1"
                     /translation="MTAYIIRRLLLLPLIMIGVTLIIFSMIWALGPDRLLASYVKSPE
                     ALKTPDAAERLIKKYGLDEPMPVQYLKWLGNILQGDFGYSMVGKKGVLPSMLERFPYT
                     LELTIYAIVPVILVAIWLGVISAVNQNKFTDQFIRVFALVGWSLPDFVFGLLLLLVFY
                     SILGWFPPGMVSTEFDLVMRSDEWRTITSMPTFDALLNGRLDIFIDALRHIILPVLTL
                     AYLWWAYLLRITRSSMLEVLRKDYIRTARAKGVPERTVIKKHAKRNAMIPVITVAGGS
                     VMGLLGGTVFVETIFSRVGMGRFLADAASLLDYWSIIGGALFFSFIMVVGNLIVDVSY
                     ALVDPRIRLE"
     gene            complement(5109..6950)
                     /locus_tag="PKL93_08055"
     CDS             complement(5109..6950)
                     /locus_tag="PKL93_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71249.1"
                     /translation="MRKILLVTLTVLFLSLVAFASDPDTFVNLTIGEPETLDPLHSYD
                     TASGEVILNLYDNLIQYNGESVTDYLPMISTQVPSVENGLISPEGTVYTFPIREGVKF
                     HTGNVLTPEDVKYSFHRYILGDPSGGPQWMIIEALTGGAHASVESWFEAYSGMPYSEA
                     VDADKNVVSEEARQKLISFYEEVVSPKVKIEGNSVVFTLDAPWGPFMNTIAHFGSWAA
                     ITDSKWGIENGAWDGKADGWWKWHDLEPQESPYHNADAGSGPFKLVEWDSAEMKVVLE
                     RFDDYWAGPATIKNVVIWGIDEFSTRKAIFEAGDADIAYFGAPYLDQAEALAAEGKAV
                     VTKGYPTASITSLHFNWLVAEGSEYLGSGKLDGNGVPRDFFSDIHVRRGFAYCYDGAT
                     FVDEVLNGLGELVPTDLPKGFLGYDETLPLPEFNLAKAAEEFKKAWGGEVWEKGFKIQ
                     LLYNTGNDARQTIAEMLAYFVNSINPKFKVEPLGVQWPTYLAAQRNGFLPLFTIGWLA
                     DYPDPHNFIATYYASYGAYASRQGVPFQEWAAANVDAEISAAAVSVDNEERIKLYRSV
                     QEKVIDAAIGIPLYMPTGLNVRAPWVEGWYPHLVRSGFYYYQLSKNQ"
     gene            7122..7196
                     /locus_tag="PKL93_08060"
     tRNA            7122..7196
                     /locus_tag="PKL93_08060"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7154..7156,aa:Val,seq:gac)
     gene            complement(7218..8066)
                     /locus_tag="PKL93_08065"
     CDS             complement(7218..8066)
                     /locus_tag="PKL93_08065"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNQ71250.1"
                     /translation="MSISLLQEELRDYVENVKRAARFIEKEVDSQPKVAVILGSGLGD
                     VSKSLENAVSIPYDEIPGFPLSTAPGHKGELTFGNALHHNVMLMNGRFHFYEGYSMRT
                     VTFPIRIMQELGVEVLLITNASGGLNPDFEVGRVMLIRDIINFMGDNPLIGQNVDEWG
                     PRFPDMSEPIDRELLSKALLSAKELGIGVYEGVYVGVAGPNFETPAELRMLRRFGADS
                     VGMSTVPEVIVARHAGIRVLGLSAISDRAVAEDLKPLTAEEVLEMAKKAGNDMSKLIL
                     KTLEKV"
     gene            8187..9929
                     /locus_tag="PKL93_08070"
     CDS             8187..9929
                     /locus_tag="PKL93_08070"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNQ71251.1"
                     /translation="MRFSQLYAPTLKEAPADADLISIKLLVRGGFVRKVAAGIYSFLP
                     LGLRVLKKIEDIVRQEMNAIGSQEILMPIIQPAELWHETGRWDDYGPEMMRMQDRHER
                     FFTLGPTHEEIITDLMRNELNSYKQLPVSLYQIAMKYRDEIRPRFGLMRSREFLMKDA
                     YSFHDSWPSLDETYKQFYGAYSKILQRIGLEFLVVEADSGAIGGNESHEFNVLADYGE
                     STLLYCDCGYAASDEKAEYLLREEPQERPTDELQLVPTPNSRTVDEVSAFLEVTSKDI
                     VKSLIFKGRLGYYMALIRGNEELNESKLKAALQDQTLVMATPQEVLDLLGVPIGFVGP
                     IGLPKEVTIIADHTIKGLKNAVCGALKEGYHYSGVLPGRDFKINSWHDLKMVTEGDPC
                     PKCGTPMKSAKGIEVGHIFKLGTKYSEKMNGYVTDDKGNSIHYIMGCYGWGVSRTISA
                     VVEQLHDENGIIWPLAVAPFDIIITAVNIKDKVVSEKSVQLYELLRKEGFDVLLDDRE
                     ISPGMKFKDADLIGIPIRVTLGKKLAQGIVEVKLRTEKKPLEVSIDEGFDSLLEEIKK
                     ELSNYRPVSKMEAK"
     gene            9929..10840
                     /gene="ftsY"
                     /locus_tag="PKL93_08075"
     CDS             9929..10840
                     /gene="ftsY"
                     /locus_tag="PKL93_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNQ71252.1"
                     /translation="MGVFSRLKDGLKRTREGFFKRVRKLLSLESLDSESLEEIEELLI
                     LSDMGSETAEEVIEALKDRKDKGKEPIEVLKEVLVEILDIPFEVPQARPYVVSVVGVN
                     GTGKTTTIGKLSKLYSSKGNKVVLAACDTFRAAAVEQLKIWSERAKVDFISQGQGADS
                     AAVAFDAVRHAASKGKDVVIVDTAGRLHTRSNLMEELKKVHRVIGKASPGAPNEVLLV
                     IDATTGQNGLVQAKKFKEAVDVTGIVLTKLDGTAKGGIIFAIVKELGIPVRYIGVGEK
                     EDDLKPFDSREFVNALLSTGEESSEEA"
     gene            10843..11307
                     /locus_tag="PKL93_08080"
     CDS             10843..11307
                     /locus_tag="PKL93_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNQ71253.1"
                     /translation="MGFTLKSSYALRALYKMAKSTREGKDKLSLVEIVQGNEIPRDFL
                     EKIFGELRQAGIIKAVRGRYGGYCLARPANEILLRDVILKLDKPMNSYPCLQQNGKCL
                     EDPNCTVKYVWFKLYNAMMRELGSMTLGNLLEYGDKIAQNPLESDELKENLE"
     gene            11355..11846
                     /gene="coaD"
                     /locus_tag="PKL93_08085"
     CDS             11355..11846
                     /gene="coaD"
                     /locus_tag="PKL93_08085"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNQ71254.1"
                     /translation="MASNKMKAVYPGSFDPITYGHIDLVERCSKIFDEVVVLVMENVN
                     KRHFFSHEERIGMVRCGVSHLENVTITTYNGLLVDFARASDVRILIRGLRAVSDFELE
                     LQMAHANKAMLPELETLFLMTDTSNSFISSSMVREIAAFGGDISKWVPPCVQEEFKRK
                     LGK"
     gene            11925..12530
                     /gene="tsf"
                     /locus_tag="PKL93_08090"
     CDS             11925..12530
                     /gene="tsf"
                     /locus_tag="PKL93_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNQ71255.1"
                     /translation="MANITSDMVKKLRDATQAGMMDCKKALVETDGDFDQAKDYLRKK
                     GILKADKVSGRETAEGLVYSYIHHNSKLGVLLELNCVTDFVARMEEFKELAHKIALQL
                     AAMGARFISREHVPQEIIDKEKEIYTEQMKDSGKPANIIERIVESKLESFYKDNCLIE
                     QEYVFESGKTINDLLVELIAKTGENIRISRFVRWQVGESEN"
     gene            12576..>13005
                     /gene="pyrH"
                     /locus_tag="PKL93_08095"
     CDS             12576..>13005
                     /gene="pyrH"
                     /locus_tag="PKL93_08095"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730102.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNQ71256.1"
                     /translation="MYKRVLLKLSGEVLSGEGARGFDSERTSFLIEELRPVIEKGIQL
                     AIVIGAGNIIRGRELSNMRGSRADELGILGTIMNALYLKEALCVNGFETIAVSSIAGI
                     SSLVEHRYDTIEDALNEGKIVVFGGGTYLPFFTTDTAAAVR"
     assembly_gap    13006..13105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13106..13432
                     /gene="pyrH"
                     /locus_tag="PKL93_08100"
     CDS             <13106..13432
                     /gene="pyrH"
                     /locus_tag="PKL93_08100"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491442.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNQ71257.1"
                     /translation="GGGTYLPFFTTDTAAAVRAVEIGADVLIKGTKVDGVYDKDPKVN
                     GDATKIDKTTFSEAIEKGLGIMDIEAFSICQRYGLSVRVIDFFNKGNLFDAIIGGKTG
                     TVVLPD"
     gene            13447..14730
                     /gene="eno"
                     /locus_tag="PKL93_08105"
     CDS             13447..14730
                     /gene="eno"
                     /locus_tag="PKL93_08105"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNQ71258.1"
                     /translation="MYAQIVDLVAREVLDSRGTPTVEAEVWLDDGGHGRAIVPSGAST
                     GKFEALELRDGDKKRFLGKGTLKAVENINEVIAPEIIGMNAFDQIALDAALLELDGTE
                     NKDKLGANAILAVSMAVARAAADSIDTPLYKYLGGVNAKALPVPFMNIVNGGKHADNN
                     LDIQEFMIVPAGFKYFRDALRAGVEVFHNLKKLLKDSGHVTAVGDEGGFAPSFENNEA
                     ALKYIINAIDAAGYKAGEEIMIALDCAANEFYNEDKGLYNIDGKELTPDQLIEYYSNL
                     TKRYPIISIEDPFAEEDWESFAKFTKAIGDEIQIVGDDLFVTNIKRLEKGVEKKSANS
                     ILIKLNQIGSVTETLNTIEYAQKHGMTCVISHRSGETEDTFISHLAVAANTGMIKTGS
                     ASRTDRIAKYNELLRIEEELGEQAEFRGRESFYNL"
     gene            14790..15458
                     /gene="sdaAB"
                     /locus_tag="PKL93_08110"
     CDS             14790..15458
                     /gene="sdaAB"
                     /locus_tag="PKL93_08110"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HNQ71259.1"
                     /translation="MLLDIIGPAIVGPSSSHTAGMARLGRAFRSLLAKVPNEVRFVLN
                     SPLFATFRGHGSDRALVGGIMGIKESDPMLRDSFQIAKDKGIYFTFEEEDMGDLHPNT
                     VKINGSSGELFLSVTGASVGGGSIKITAIDDFDTDVTGKYPSLVILNADIPGALSQIV
                     GEISSSDMNISNLFLSRVDPFRHEALCVVELDNEPDEHLLRAIKRLEVVKKVSYLERL
                     DSLV"
     gene            15455..16336
                     /locus_tag="PKL93_08115"
     CDS             15455..16336
                     /locus_tag="PKL93_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNQ71260.1"
                     /translation="MIMKFEDLVKAARDSKTPLHKVVSNWYMMNTGNDPRFAEDTAKE
                     LVGEMMNSYRSRLEKPEKSLTGWVGDNDEKARNHSPEFLSSLVYSGVGIALSMSEHNA
                     SMGKIVACPTAGACGVVPGALLSLHINRNIDLETLGNALLVSGAVGERIRKRASISGA
                     VSGCQAEIGTATAMASVAIVYATHPEEYSALENAPALALKSLMGLVCDPVGGFVEIPC
                     VKRNAFGVSIAFTAAELALAGIKSAIPFEEVADSLGRVGRRLPEELKETARGGIAITP
                     TAKKMVADFLGGVDKKV"
     gene            16419..16679
                     /gene="rpsO"
                     /locus_tag="PKL93_08120"
     CDS             16419..16679
                     /gene="rpsO"
                     /locus_tag="PKL93_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNQ71261.1"
                     /translation="MEVNKQELVKEYQIHDNDSGSTEVQIAILTARIRHLTEHLKKHP
                     KDFHTRRGLLKLVGRRRKMLRYIKTKKPEVYLELINKLGLRG"
     gene            16785..>17566
                     /locus_tag="PKL93_08125"
     CDS             16785..>17566
                     /locus_tag="PKL93_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNQ71262.1"
                     /translation="MSYKRWEREFFGQKLVIENGKMAKQADGAVVLRYADSVLLTTVN
                     GNEQAVPGTDFLPLTVEYQEKFYAAGKIPGGFLKRESRPSDNAVLSARIIDRPIRPLF
                     PDGMRNEIQVIVTVLSADPDNPPDIWGIMASSLALNISPIPFEGIVAGVQVGYIDGKY
                     VVFPSADELERSELDIVVAGTEFAVAMVEGEAKEVSEEVMVGALEAAHEAIKSLVAFQ
                     KEIISEFQIEKWELEIPAAPEGFLESFETLVDRNELSEIMLTP"
     assembly_gap    17567..17666
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17667..18608
                     /locus_tag="PKL93_08130"
     CDS             <17667..18608
                     /locus_tag="PKL93_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNQ71263.1"
                     /translation="SLKNIVPNGESFPYTIRVVSEVLESNGSSSMATVCSGSLSLMAA
                     GVPMEKHVAGVAMGMIQEPEKTVVLTDILGNEDHMGDMDFKVTGTRDGITAFQMDVKV
                     SGVSSEIMTKALEQARLARLKILDLMYEAIPEPRKFVSDYAPIIRTINLPYEKIGEII
                     GPGGKVIKKLSSEYDSTIFIDDERSQAKIVGSNREKLDQLEKVIDAIISEVKPGQLFE
                     GKISRAEAYGFFVEIAPGKTGLLHISKMGASGKDFLREHKVGDTIAVEVAGTDQMGKI
                     SLKLEGVEVKEDSKRDNKRPYHRNDRNDRNRGDRNRK"
     gene            18616..>19103
                     /locus_tag="PKL93_08135"
     CDS             18616..>19103
                     /locus_tag="PKL93_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNQ71264.1"
                     /translation="MNNHYIELPNGAVIIGERKEETRTVSMAFAMKVGSADEEDSISG
                     VSHFIEHALFKGTLRRNAFEIKEPIERIGGSLNAYTGRVSTVYYAKVPDTYASEALEI
                     LFDLITSPRFDEASLELERGVILEEIASAEDDPYDRIYDMTIEKVWDRDFGRPILGYQ
                     DTV"
BASE COUNT         5397 a         4229 c         4521 g         4756 t
ORIGIN      
        1 cacactgttc ggtttcaatg acctaccata aggccgacaa ttcactgaag tgtcattact
       61 gcggttacaa agagccgatt ccaaagacct gtcccagctg cggatcaatg acgctttctg
      121 caaggggctt tggaacggag agagtcgaac acgatcttca aaaatacttc ccttccgtga
      181 agattatgcg gatggaccgg gagactatcg ataacccaat agcttacgaa aaagctcttc
      241 ttgagatctc aaagaaggag tctcagataa tcgttggaac aaagatgatc acaaaaggtc
      301 ttgacttccc agacgttgaa atggttctcg tggtcgatgc agacagactg atgagtttcc
      361 cgagttacga tagtcccgaa actgcattcc agcacatatc tcaagtcagc ggacgctccg
      421 gcagagccgg catagggaaa gccttcattc agagtttcaa cccgaagaac aggataatca
      481 aagcggcatt cgagagagac tacgaagcct tctacaaaga tgagatagct ctcaggaagg
      541 agctaaacaa tccgccattc gctaagatcg cagagatcat atgctacgga gatacggaag
      601 acaagagcgg tgagctggca gagaggattc taagtgaagt caaagcagaa agactcgata
      661 caatagaggt cttcggccct ctcacaccac tgctttcgaa gcttaaaaac tcctacagaa
      721 tgaagataac gataaaactt ccttcagata gtgattgcgg atttctcgaa aagatccaga
      781 agagacatcc cgccgatgtc cagataataa taaacggaat cggcggaatt atatagtctt
      841 aaaaccctct tttcagttct cccgggaaga agcaggaaac gtaatgacct ccgccaatat
      901 cttgcagctt aggctcttct ttggagcaga tatcctttgc aattggacat ctcggatgga
      961 atctgcaacc ggttggcggg tttataggac ttggcacatc cccttcaaga agaattctct
     1021 gcttcttttt ctcggggtta gcctctggaa ttgccgacat tagagagaca gtataaggat
     1081 gaagtgggtt atcgaaaagc ttctttttgt ctgcaagttc ggctattttc ccaagataca
     1141 tgacggccac tcgtttgctt acgtgcttca caacgccaag atcgtgggca atgaagagat
     1201 aagtgaggcc aaaatccttt tgcagatcgc tgaggagatt aagaatctga gcctgaatcg
     1261 atacgtcgag agccgagacg gcttcgtcac agacaatgag cctgggattc aaaatgagag
     1321 ctctggcaac gcctatacgt tgcctctgac caccgctgaa ctcgtggggg aatctagaca
     1381 tgtgatcctt tgacagacct acttttgtca taatgtcggc cactttgtcc ataacatctc
     1441 tgccggtagc gagtccgtga attcttgcac cttcgccaat aatattcttg actctcatcc
     1501 tgggatttaa tgaagagaag ggatcctgga aaattatctg agcatctctt ctgaatgctt
     1561 tcctcttttc ctctctgttt cccagaagac tctttatgaa ttccccggcg tcctcgttga
     1621 attctttgaa gtacttctcc gccatttttt tgtcaaagcc atcttcgatg gagctgatag
     1681 cgccttcgac cgatcccgac ttctctttaa ggtcagaaaa cttctcgatg taagtttgct
     1741 taatatactt tctggctttg agagaaggca tgaagaaatg ggtagtgtct tcgtcgccca
     1801 caattattct tcccgaagtc ggctcgtaaa gcctgagaac agtcataccc gctgtggtct
     1861 ttccgcagcc ggattcaccg accagtccaa gagtctcctt ttcgtaaaca ctgaagctta
     1921 cgtcatcgac agctttgacc tgagcaacga ctcttctaaa aacacctgcc ttcaccggaa
     1981 agtacttcac taaattatcc gttctgagga gaagtttgct tttgttatcg ttgctcatgc
     2041 gctctcacct gcccttgcca ctttgatcgt ttcgataagt ttatctatgt gccagcaggc
     2101 agctgtatga tcattttcta tctcaaccaa cggtggttct tcctttcgac atttttccgt
     2161 tgcgagagga catcttgtgt tgaatctgca tccatcgggg aaatctagag gatcggggac
     2221 tacaccggga atattataga gaacctcttt gtcctcatcc agcctgggga tcgcgttcat
     2281 aagcccccat gtgtaaggat gccttggatt tttgaacaaa gtgtgtacat cggcgtattc
     2341 aacgaccttt cccgcataca taaccaccac tctctgagcc atctcggcta ttaccccgag
     2401 atcgtgagta atcataacga gtgccatgcc atattgatct tgtaaagact tcatcagctc
     2461 gagaatctga gcctgaattg tcacatccag agccgtggta ggttcgtcgg caaagagcag
     2521 tttaggattg caggacaaag ccattgcaat catcgctctc tggcgcattc ctccggaaag
     2581 ctcatgaggg tattcatcta tccttttctc cggttcagga atcccgactt tcttgagcat
     2641 atctatggcc atttgccggg ccttcttttc atctacatcc tgatgaagca gaatcgcctc
     2701 catgatctgg aatcctattg taaagactgg attgagagca gtcatgggtt cctgaaagat
     2761 catggccatt tcgtttcccc tgatcttcat catcttcgat tcaggaattg ccatgacgtc
     2821 ttcgccgtcg aagatgatct tgccacctgc tatctcaccc ttttcgtcaa gaagtctcat
     2881 aatagagagc gatgtcacgc tctttccgca acccgattct ccgactattc cgagagtctc
     2941 acccggaaag acgtcgaatg tgactccatc gacggctttc acgattccat cttctgtgtg
     3001 gaaataagtt cttaaatcct caacctgcaa cagtgctttc ctttcattca tgactcaaac
     3061 ctcctcagct tctcattctc gggtcaaaga tgtccctaag ggcatctcca acaagattcc
     3121 acgcaagaac gaagagcacc atggcagtac cagggaaaac aatggcaaac cacgactggt
     3181 cgagcatagt catccagttc cttgaataag aaagtattgt tccccagtcg gcataacccg
     3241 gttctgcacc aactcctaga aagctcagtc ctgctgcagt tatgacataa gatccaattc
     3301 tcattgacat ctgaacaacg actggaaaaa tcgtgttggg aaggatatgt ttaataatga
     3361 tcttccaatc tttctgtccc agagccttgg cagcgagaac atattgttct tctctagctt
     3421 gaagaatgtt tcccctaatg agacgtgcag tgctcatcca gccgaagatt atcagcgcga
     3481 ttatgacttt atctagcccc tttcccaaaa ccgtagtcat taccatagct gcaactagga
     3541 atgggattga caagaatatg tccgttatcc tcattaggat ttcgtcaacc catcctccga
     3601 aatacgcgga tattgagcca acaatcaccc caataagggc agccatactg gtaattatca
     3661 aacccagtct aaaagccgtt ctagtccccc agattacgcc gtagaagaca tccctcccac
     3721 cgattactcc gaagggatgt tctgcagaag gcgctattgg ttttgatgac caggaagctc
     3781 ttggaatctg atagttatcc cccatctcat tgacgcctgc tatctgaggg gcaaatattg
     3841 caatgattat gaagaagatc aacagacaag taccaagaac agcggttccg ttcgtccagt
     3901 atttcttcat gatctttcta aattcactct ttctctttgc cggcatctgc attttccccc
     3961 tcattcaagc cttattctgg gatcaaccag agcgtaagaa acgtctacga tcaggtttcc
     4021 aacaaccatt atgaaggaga agaacaatgc tccacctata atcgaccagt aatccagaag
     4081 acttgctgca tcggcaagga atcttcccat tccgacgcgg gagaagatcg tctctacaaa
     4141 gactgttcct cccaacaacc ccataaccga tcctccagca accgtaatga ctggaatcat
     4201 agcgtttctc ttggcatgct ttttgataac cgttctttcg ggcacacctt ttgctcttgc
     4261 cgtccgtata tagtccttcc tcagcacttc aagcatgctc gaacgcgtaa tccttagtag
     4321 ataagcccac caaaggtacg caagggtgag gacaggaagt ataatgtgtc tcaatgcatc
     4381 gatgaatatg tcaagtctcc cattcagtag tgcgtcaaat gttggcatac ttgttattgt
     4441 tctccattca tctgatctca tgaccagatc aaactccgtg cttaccatac ccgggggaaa
     4501 ccaaccaaga attgagtaga agacaagaag gagcaaaagc ccaaaaacaa aatcaggaag
     4561 agaccaaccc acaagggcaa agacccttat gaattgatct gtaaatttgt tctggttaac
     4621 ggcagaaatc actcccaacc atattgcaac aagaattaca ggaacaattg catagattgt
     4681 cagctccagt gtatacggga atcgctcaag catcgacggg agtacccctt ttttcccaac
     4741 catggagtac ccaaaatcac cttgtaggat atttccaagc catttcaagt attgaacagg
     4801 cattggttca tcaagaccgt atttcttgat cagtctctct gccgcatctg gagtcttcaa
     4861 agcttcagga ctctttacgt aagaggcaag aagcctatcg gggccaaggg cccagatcat
     4921 agagaaaata atcagagtaa cacctatcat tatcaatggc agtagaagaa gtctcctgat
     4981 tatgtaagca gtcacccgaa aatcaccact ctttcttcaa aacaaagaca atagtttatt
     5041 ataatctata tctctggtca aaaaaagagc cgggcaagaa aaacccggct ctctacggga
     5101 tatttcaatt actgattctt ggaaagctgg taatagtaga atccagacct tacaagatga
     5161 ggataccagc cctcaaccca aggcgctcta acgttgagac ctgtcggcat atacagagga
     5221 ataccgattg cagcatcaat taccttttcc tgaacgcttc tataaagctt tatacgttct
     5281 tcgttatcaa cagatactgc agcagcgctt atttcggcat caacatttgc ggctgcccat
     5341 tcctggaatg gaactccctg tctggaagca tatgcaccgt aacttgcata gtaagtagct
     5401 atgaaattat gtgggtccgg atagtcagcc aaccagccta ttgtgaacaa cggaaggaaa
     5461 ccgtttctct gagccgccag atacgtcggc cactgtacac caaggggctc aaccttgaac
     5521 ttcggattga tcgagttcac aaagtaagcg agcatctcag caatagtctg tctagcatcg
     5581 ttgccagtgt tatagagaag ttgaatctta aaacctttct cccagacttc tccgccccag
     5641 gccttcttga actcttcagc agcctttgcg agattgaatt ccggcagagg aagcgtttcg
     5701 tcatatccaa ggaaaccttt gggaaggtct gttggaacaa gctctccaag gccattcaat
     5761 acctcgtcta caaaagtggc tccatcgtag cagtaggcaa atcctcttct aacatggata
     5821 tcagagaaga aatctcttgg cactccgttt ccgtctagtt ttccactgcc taggtattca
     5881 ctaccctctg caacgagcca gttaaagtga agggaagtga tacttgcggt cggataaccc
     5941 ttcgtgacaa ctgctttccc ctctgctgca agagcctcag cctgatctag atatggagca
     6001 ccaaagtatg caatatcggc atctcctgct tcaaatatcg cttttctggt tgagaattcg
     6061 tcaattcccc agattacgac gttcttgatc gttgcagggc ctgcccagta gtcgtcaaac
     6121 ctctcaagaa ctactttcat ctctgcagaa tcccattcaa caagcttgaa tggtcctgaa
     6181 ccagcatcag cattatggta cgggctctcc tgtggttcga gatcatgcca cttccaccag
     6241 ccgtctgctt tgccgtccca tgctccattt tcgattcccc acttggaatc agttatagct
     6301 gcccaagaac caaaatgagc tatcgtgttc atgaaaggtc cccaaggagc atcaagagta
     6361 aatacaacac tgtttccttc tatcttgacc tttgggctta caacttcctc atagaaggaa
     6421 atcagtttct gccttgcttc ttcagaaacg acgttcttgt cggcatcaac tgcttctgaa
     6481 tatggcatac ctgagtaagc ttcgaaccag ctttcaacgc ttgcgtgagc gccaccggtt
     6541 agagcttcga tgatcatcca ctgaggtcca ccagacgggt caccaagaat atacctatgg
     6601 aaagaatact tcacgtcctc aggagtaaga acgtttccag tgtggaactt aactccttcc
     6661 cttatcggga atgtgtatac ggtcccctct ggactgatca ggccgttctc cacagaaggt
     6721 acttgtgtag aaatcatcgg caggtagtca gtaacagact caccgttgta ctgaatcaag
     6781 ttgtcataca gattcaatat tacctcacca ctggccgtat cataagagtg aagcggatct
     6841 agagtttccg gttcaccaat agtgagattc acaaatgtgt ctgggtcact agcaaacgca
     6901 actaacgata agaaaagtac agtaagtgtt accagtaaaa tctttctcac taatgttgcc
     6961 ccctttcaaa tagagatgag tgtgatagta caattataat ctatggagcc tgaaatttca
     7021 aaataccgtt aacctatacg tattatttcg caatccaaag caatttcgtt tgttcttgta
     7081 ttgacaacgc tcttaagacg atggtagaat cattcttgtt cgggtgcgta gctcagtgga
     7141 agagcgcttc cttgacgagg aagaggccgt aggttcaatc cctaccgcac ccaccaaagg
     7201 ggagagttta gctctcccta tactttttct aaagtcttca gtatcaattt actcatatca
     7261 ttgccggctt tcttagccat ttccaggact tcttcggcag ttagcggctt tagatcttcc
     7321 gctacggccc tgtcggaaat cgccgatagt cccaatactc taatacctgc atgccgggca
     7381 acgatgacct ctggaactgt ggacataccc acggaatccg caccgaatcg tctcagcatt
     7441 ctcagctctg ctggagtctc gaagtttggc ccggccactc caacatatac accttcatac
     7501 actccgattc caagttcttt ggcagaaaga agagctttgc tcaaaagttc tctgtcaatc
     7561 ggctcactca tatccggaaa tcgcgggccc cactcgtcaa cgttctgacc tattagaggg
     7621 ttgtcgccca tgaaattaat gatatccctt ataagcatga ctcttcctac ttcgaagtcc
     7681 ggattcagac caccggaagc gtttgtaatt aggagaacct ccacgcccaa ttcctgcatt
     7741 atccttatgg ggaaggtgac tgttctcatc gaataacctt catagaagtg aaagcggcca
     7801 ttcataagca ttacattatg atggagagca ttaccgaaag tcagttcgcc cttatgaccc
     7861 ggagcggtag atagcgggaa accagggatc tcatcgtaag gaattgaaac ggcattttca
     7921 aggctcttcg acacgtctcc aagaccgctg ccgagaatca ccgccacttt aggctgcgaa
     7981 tcgacttcct tttctatgaa acgagcggct cttttgacat tttctacata gtccctaagc
     8041 tcttcctgaa gcaaggaaat actcatgctc gtcctcctta taagacatat acttcattat
     8101 atcaaaacgt caaacagctt gctggaacta tgctataatc tcactgaatt agtcctgatt
     8161 agctcatttt cgagaggaga gatctgatga ggttttcaca gctttacgcc ccaacattga
     8221 aagaagctcc cgccgacgcc gacttgataa gtatcaaact actcgttcgt ggtggatttg
     8281 tgagaaaagt agcggcaggc atctattcct ttcttccgct cggtcttcga gttctaaaga
     8341 agattgaaga tatcgttcgt caagagatga acgctatcgg ttctcaagaa attcttatgc
     8401 cgatcattca accggcagag ttatggcatg agacaggtag atgggacgac tatggtccgg
     8461 aaatgatgag aatgcaggat aggcatgagc ggttcttcac gctaggaccc actcatgaag
     8521 agatcatcac tgatcttatg agaaacgagc ttaattcgta caaacagctt cccgtttcgc
     8581 tttatcagat agctatgaaa tacagagatg agattcgtcc cagattcgga ctcatgagat
     8641 cgagagagtt tctaatgaag gacgcctata gttttcatga ctcatggccc tcattagatg
     8701 agacctacaa gcagttctac ggtgcataca gtaaaattct ccagaggata ggactggaat
     8761 ttctggttgt agaagcggat tccggagcta tcggcggtaa tgagtctcat gaattcaacg
     8821 ttctagccga ttacggtgaa tctacgcttc tctattgcga ctgtggctac gcagcttccg
     8881 atgaaaaggc ggaatatctc ttaagggaag aacctcaaga aagacccaca gatgagttgc
     8941 aactagttcc tacgcctaac tcgaggacgg tagatgaggt ttcggctttc cttgaagtaa
     9001 cctcgaagga tattgtgaag tccctaatat ttaaaggtag actgggctac tatatggctc
     9061 tgattcgcgg aaacgaagag ttgaatgaat cgaaattgaa agctgctctt caggatcaga
     9121 ctcttgttat ggcaactcct caggaagtgt tggatttact gggagttccg atcgggtttg
     9181 ttggtccaat tggtcttcca aaagaagtca ctataatagc agatcacaca ataaagggtc
     9241 tgaaaaacgc ggtttgtgga gcgttgaaag agggatacca ttattccggc gttcttcccg
     9301 gacgagactt caagatcaat tcctggcacg atctaaaaat ggtcaccgaa ggagatccct
     9361 gcccgaaatg cggtacaccc atgaaatccg caaaaggcat cgaagttgga catattttca
     9421 agctcgggac gaaatattcg gaaaagatga acggatacgt gaccgacgac aagggtaata
     9481 gtattcatta tattatgggc tgttatggtt ggggcgtgtc tagaacaatt agcgccgtag
     9541 ttgaacagct tcacgatgaa aacggtatca tctggccgct agccgtagct ccgttcgata
     9601 tcatcattac tgcggtcaac ataaaggata aggtcgtttc ggagaaatcc gtacaacttt
     9661 acgaacttct gaggaaggag ggcttcgacg ttctcctaga tgatagagag atatctcccg
     9721 gaatgaaatt caaagatgcc gaccttatag gtattcccat aagagtgaca ctcggtaaga
     9781 aactcgccca aggtatcgta gaagtaaaac tgagaacaga gaagaaacct cttgaagtaa
     9841 gtattgatga gggattcgat agccttctgg aagagataaa gaaagaactc tctaattaca
     9901 gaccagtaag caagatggag gccaaataat gggagttttc tcaagactta aagacggttt
     9961 gaagaggacc agagaaggct ttttcaaaag ggtaagaaaa cttctctctc ttgaatcgct
    10021 tgattctgaa tcacttgaag agattgagga gctcctgata ttgtccgata tgggttccga
    10081 aacggctgag gaagtcatcg aagctttgaa agatagaaaa gataaaggga aggaacccat
    10141 tgaggtactg aaagaagtcc ttgtcgagat acttgacata ccctttgagg tgccacaagc
    10201 gagaccttac gtagtttcgg tggtcggcgt taacggtacc gggaagacaa ccacaatagg
    10261 gaagctttcc aaactctact ctagcaaagg aaacaaagtg gttctagcag cctgtgatac
    10321 ttttagagca gccgcagtag aacaactgaa aatatggagc gaaagagcca aagttgactt
    10381 cataagccag ggtcagggcg ccgattccgc agcagtggcc tttgacgctg taaggcacgc
    10441 agcaagtaag ggaaaggatg tagtcattgt cgacacggca ggcagattgc acacccgaag
    10501 caatctgatg gaagaactga agaaagtaca tagagtgatc ggcaaagcat cacccggagc
    10561 tccaaatgaa gtgttgctag taattgatgc aactacggga cagaatggcc tggtacaggc
    10621 gaaaaagttc aaagaagctg tcgatgtcac cggaatcgta ttgacgaagc ttgatggtac
    10681 cgctaagggt ggaatcattt ttgcgatcgt caaggaactc gggataccgg tgagatatat
    10741 aggcgtagga gaaaaggaag atgatttgaa acccttcgac agcagggaat tcgtgaacgc
    10801 tcttttgtcc accggagaag agagttcaga ggaggcttag aaatgggttt tacgcttaaa
    10861 agcagttacg ctttgagagc actttataag atggcaaagt cgacccgcga aggaaaggac
    10921 aagctctcgc tcgtcgagat cgtccaggga aatgagattc cgagagattt tctggagaag
    10981 atcttcggtg aactgagaca ggcggggata ataaaagccg taagaggcag gtatggcgga
    11041 tactgtctgg cgagacctgc aaacgagata ctattgagag acgttatact gaagctcgat
    11101 aaacctatga attcatatcc atgtcttcag caaaacggaa agtgtcttga agacccgaat
    11161 tgtactgtta agtatgtttg gttcaaacta tacaacgcaa tgatgagaga actaggttcc
    11221 atgacactcg gaaatcttct cgaatacggt gacaaaatcg cccagaaccc tttagaatca
    11281 gacgagttaa aggagaatct cgagtagaag actttgaaag gaggggcgcc tgaccgatag
    11341 atagtggagt atagttggcg tcgaataaaa tgaaagccgt ttatccaggt tcctttgatc
    11401 cgataacata cggtcacata gatttggttg aaagatgcag caaaatcttt gacgaggttg
    11461 tggtgctcgt catggaaaac gtaaataaga gacatttttt cagtcatgaa gagagaattg
    11521 gaatggtaag gtgtggcgtg tctcatcttg agaacgttac tattacaacg tacaacgggc
    11581 ttctcgttga tttcgctaga gccagcgatg tgaggatttt gattcgaggc ctcagagccg
    11641 tgtcagactt cgagcttgag cttcaaatgg ctcacgccaa caaggcaatg cttcctgagc
    11701 tcgaaactct ttttttaatg acagacactt ccaactcctt tatctcttct tcgatggtcc
    11761 gagaaatcgc agcattcggc ggggacatct ccaaatgggt tcccccttgc gttcaggaag
    11821 aattcaagag aaagctcggg aaatgactta acatcacgga aattattaat tgcacaactg
    11881 ggttataatt tttcttggaa ttttaatgga ggaggacata aaaaatggca aatatcacca
    11941 gcgacatggt aaagaaactc cgtgatgcca ctcaagcagg catgatggac tgtaaaaagg
    12001 cgttggtcga aaccgatggt gatttcgatc aggcaaagga ctatctcagg aaaaagggca
    12061 tactgaaggc agataaggtt tcgggaagag agacggccga aggacttgtt tattcttata
    12121 ttcatcacaa cagcaaactt ggggtacttc tcgagctcaa ctgcgtaacc gacttcgtag
    12181 cgagaatgga agaattcaaa gagctagctc ataagatcgc tctgcaactg gctgctatgg
    12241 gagcgagatt catctcccgc gaacacgtgc ctcaggagat catagataaa gagaaggaaa
    12301 tatacactga acagatgaag gatagcggga aaccggcaaa cataatagag agaatcgttg
    12361 aaagcaagct cgagagtttc tacaaagaca actgccttat cgagcaggaa tacgtttttg
    12421 aaagcggcaa gacgataaat gatctccttg ttgagttgat tgcaaagacc ggcgagaaca
    12481 ttagaatcag cagattcgtc cgctggcaag taggagagtc tgagaactga gtttgaaagg
    12541 tgagccgggt gctcaccttt ttttcggagg tgcttatgta caagagggtg ctcttgaaac
    12601 tcagcggtga ggttttgagt ggagaaggcg caagaggatt tgattcagaa agaacttcct
    12661 ttctcatcga agagctccgc ccggtaattg aaaagggtat tcaactcgca atagttatcg
    12721 gcgccgggaa tatcattagg ggtcgtgaat tgtcgaatat gagagggtcg agagccgatg
    12781 agcttggaat actaggcacc ataatgaatg ccctgtattt gaaggaagca ctctgcgtaa
    12841 acggtttcga aacgatagct gtctcctcga tcgcagggat ttcttctcta gttgagcaca
    12901 gatacgatac cattgaggat gctctaaatg agggaaagat agtcgttttc ggcggcggga
    12961 cctacctgcc ttttttcacg acggacactg ctgccgctgt cagggnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnggcgg cgggacctac ctgccttttt tcacgacgga
    13141 cactgctgcc gctgtcaggg cggtcgaaat cggtgccgat gttttgataa agggtacaaa
    13201 ggttgatgga gtgtatgaca aagatcctaa ggtgaatgga gatgccacca agattgacaa
    13261 gacaactttc tctgaggcta tcgaaaaggg gttaggaatc atggacatcg aagccttctc
    13321 gatctgccaa agatatggat tatccgttag agttatagac ttcttcaaca agggtaacct
    13381 gttcgatgct ataataggcg ggaaaacagg tacggtagtt ttacctgatt aattggaggt
    13441 ggagaaatgt acgctcaaat cgttgatcta gtagccaggg aggtacttga ttcaaggggg
    13501 actccaacag ttgaggccga ggtatggctt gacgacggtg gccatggcag ggcaatagtt
    13561 ccttcaggag cttcaactgg gaagttcgag gctcttgaac taagagatgg agacaagaag
    13621 agatttctcg gaaagggcac gctgaaggcg gttgaaaata taaacgaagt aatcgctcct
    13681 gaaatcatag gaatgaatgc ctttgaccag atagctctcg atgcggctct tctagagctc
    13741 gatggaactg aaaacaagga caagcttgga gccaatgcaa ttcttgccgt ctcgatggca
    13801 gttgcacgtg ccgcggccga cagtattgat acaccgctgt acaaatacct gggcggagtc
    13861 aatgcaaagg ctcttcccgt accgtttatg aacatcgtga atggcggaaa acatgcagac
    13921 aacaacctgg atattcagga gtttatgata gtgcccgccg gtttcaagta ttttagggat
    13981 gcccttagag ccggagtaga agtcttccat aatctgaaga agctgttgaa ggatagcggt
    14041 cacgtcactg cagtaggtga tgaaggcgga tttgctccga gttttgaaaa taacgaggcc
    14101 gcgctgaaat acatcataaa tgctatagac gctgccggtt acaaggcggg agaagagatc
    14161 atgattgccc ttgattgtgc ggcaaacgaa ttctacaacg aagacaaggg actttacaac
    14221 atcgatggaa aagagcttac tcccgaccag ctgatagaat actactcgaa tctcacgaag
    14281 agatatccaa taatttcgat tgaagaccca tttgccgaag aggactggga aagctttgcg
    14341 aagttcacaa aagcgatagg ggatgagatt cagatagtgg gagacgacct tttcgtcacc
    14401 aatattaaga gactcgagaa aggtgtcgaa aagaaatcgg ccaattcaat tctcattaag
    14461 ctcaaccaga tcggatctgt caccgagact ctcaacacga tagagtatgc gcagaagcac
    14521 ggaatgacct gtgtaatctc acacagatct ggagaaactg aagatacttt tatttctcac
    14581 cttgccgtag ctgctaatac gggcatgata aagacaggtt cggcatcaag gactgacaga
    14641 atcgcgaagt ataacgagct tctgagaatc gaagaagaac ttggagaaca ggcagagttc
    14701 agaggaaggg aatccttcta taatctctaa ttgataatca ttatagcgcg gggcctgtaa
    14761 ggttccgcgt tttcattgga ggaagagcga tgctcctaga catcattggt ccggcaattg
    14821 tcgggccgtc gagctcccat accgccggaa tggctaggtt aggcagggcc tttcggtcac
    14881 tccttgccaa ggtgcctaac gaagtgagat ttgttctcaa ctcaccgctc ttcgcaactt
    14941 tcaggggtca cggttcagat agagctctcg tcggcggaat aatggggatc aaggaatccg
    15001 atcccatgct gagagattcc ttccaaatag cgaaagacaa gggcatctat ttcactttcg
    15061 aagaagaaga catgggcgac ttgcatccaa acacggttaa gatcaatggg agttccggag
    15121 aactcttctt gtcagttaca ggagcatcgg tgggtggcgg ttcaataaaa attactgcga
    15181 tagatgattt cgacaccgac gttactggaa agtatccctc gctggtgata ttgaatgcgg
    15241 atattcccgg ggctctttct caaatcgttg gggagatttc ttcgagcgat atgaacatca
    15301 gcaatctctt tctttcaaga gttgacccgt tcaggcacga ggctctttgc gtagtggaac
    15361 tggataatga acccgacgag catctgctta gggcaataaa aaggcttgaa gtcgtgaaga
    15421 aagtatccta tctcgaaagg ttggattcac tcgtatgata atgaagtttg aagacctggt
    15481 gaaagctgca agagactcga agacaccgct tcataaagtc gtctctaact ggtacatgat
    15541 gaatactgga aatgatccaa gatttgccga agatactgca aaagaactgg tcggagagat
    15601 gatgaattct taccgcagca ggctcgaaaa acccgagaaa tctcttaccg gctgggtcgg
    15661 agataacgat gaaaaggcga gaaatcacag tcctgagttt ctcagttcgc tggtctattc
    15721 aggagttgga attgccctct cgatgtctga gcacaatgca agcatgggaa aaatagttgc
    15781 atgtcctact gcgggtgcat gtggtgtcgt tccgggagcc ctcctttctc ttcacatcaa
    15841 tcgaaatatt gacctcgaaa cccttggaaa cgcattgttg gtttctggag ctgtgggcga
    15901 gaggatcagg aaaagggcgt cgatttccgg tgccgtgtca ggctgccagg cagagatagg
    15961 taccgctacg gcaatggctt cagttgccat agtctacgct acgcatcccg aagaatacag
    16021 tgcgcttgag aatgccccgg cgctcgcgtt gaagtcccta atgggacttg tgtgtgaccc
    16081 cgttggaggt ttcgttgaaa tcccctgcgt caaaagaaat gcgtttggtg tatccattgc
    16141 attcacagcg gcagaacttg cgcttgccgg aattaagtcg gccatcccct ttgaagaagt
    16201 tgccgactct ctcggaagag ttgggagaag attacccgaa gaactcaagg aaacggcaag
    16261 aggcgggatc gcaatcactc cgactgcaaa gaagatggta gcggacttct taggtggagt
    16321 tgacaagaag gtctgatcgg tttagaatag ccttgcccta ttctcggttt gaggcatagc
    16381 ccattcctag atagaggatt atatcgggag gcagatgcat ggaagttaac aagcaagaac
    16441 tagttaagga gtatcagatt cacgacaacg actcgggttc gaccgaagtt cagatcgcaa
    16501 ttcttactgc gagaatcagg catctcactg agcatctcaa gaaacacccc aaggatttcc
    16561 atacaagaag gggtcttcta aagttggtcg gaagaagaag aaagatgttg agatacatca
    16621 agaccaagaa gcctgaagtc tacctcgaac tgataaacaa actcgggctg agaggttgat
    16681 agaagtagaa atcgtctgga gcggtttacc gctccttttt tgcgtgggtt ttactcagct
    16741 gatataattg attatttgat gaaatgagaa aaagaggtgt tattgtgagt tataagagat
    16801 gggaaaggga attctttggc cagaagctcg tcatagaaaa cgggaagatg gcaaaacagg
    16861 ctgatggagc cgttgtactc aggtatgccg attcagtcct gctaactacg gtgaatggaa
    16921 atgaacaggc agtgccggga actgattttc ttcctcttac cgttgagtat caggaaaagt
    16981 tctatgcggc gggaaagata ccgggtggtt ttcttaaaag agaaagcaga ccgagcgata
    17041 atgccgttct ctctgcaaga ataatagata gacctatcag gcctctcttc ccagacggga
    17101 tgagaaatga gattcaggtt atcgtgactg tcctgtctgc cgatccagac aatccgcccg
    17161 acatctgggg gataatggca tcgtcacttg cattaaatat ttctccgatt cccttcgaag
    17221 ggatcgtcgc cggcgtgcag gtcggatata ttgatggaaa atacgttgtg tttccttctg
    17281 ctgacgaact cgaaagatct gaactcgata ttgttgttgc agggacggaa tttgcagttg
    17341 caatggtaga aggagaggcg aaagaagtct ctgaagaagt aatggtcgga gcgcttgaag
    17401 cagcgcacga agctattaaa tcgctcgtcg ccttccagaa ggaaattatc agtgaattcc
    17461 agatcgagaa gtgggagctc gaaataccgg ctgcccccga aggatttctc gaatcgttcg
    17521 aaacactggt tgatagaaat gagctctccg agataatgct cactccnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnntctc tgaagaacat tgtgcctaac ggcgaatctt
    17701 tcccatatac aataagagtc gtatctgaag tgcttgaatc caacggctcc tcttccatgg
    17761 cgaccgtgtg ttccggatca ctatcactga tggccgctgg agttcctatg gaaaagcacg
    17821 tggcaggcgt tgccatgggc atgattcagg aaccggaaaa gaccgtcgta ctcacggata
    17881 ttctaggaaa cgaagatcac atgggcgaca tggatttcaa ggttactgga actcgtgacg
    17941 gaataaccgc tttccagatg gatgtgaagg tttccggcgt atcaagcgag ataatgacaa
    18001 aagcgcttga gcaagcgaga ctggcaagac tcaagatact tgatcttatg tacgaagcca
    18061 ttcccgaacc gagaaagttt gtctccgact atgcacctat tataaggacg atcaatctcc
    18121 cttacgagaa gattggagag attatcggac ccggcggaaa ggtcataaag aaactttcaa
    18181 gcgagtatga ttcaacgatc ttcatagatg acgagagatc tcaggctaag atcgttggaa
    18241 gcaatcgaga aaaactcgat cagttggaaa aagtaataga tgcgataatc tctgaagtga
    18301 aacccggaca gcttttcgaa ggaaagatct cgagagcgga ggcctatgga ttcttcgtgg
    18361 agattgcgcc aggaaagacc gggctcctgc atatctcgaa gatgggcgcc agcggcaagg
    18421 atttccttag agagcacaag gtcggagaca cgattgccgt cgaggtggca ggaactgatc
    18481 agatgggaaa gataagtctg aagctcgaag gtgtcgaggt taaagaggac agtaagcgag
    18541 acaataaaag gccataccat aggaacgaca gaaacgacag aaaccgagga gacagaaaca
    18601 gaaagtaggt aactgatgaa taatcattat attgagctgc cgaacggcgc agttattata
    18661 ggtgagcgaa aagaagagac gagaacggtc tcgatggctt tcgcgatgaa ggtgggttcc
    18721 gctgatgagg aagattccat cagcggcgtt tctcatttta ttgaacacgc tcttttcaaa
    18781 ggaactctta gaagaaatgc tttcgaaata aaggaaccga tcgagagaat aggcggtagt
    18841 cttaacgcct acacgggaag agtctccact gtttattatg caaaggtacc agacacttat
    18901 gcctctgagg ctctggagat cctcttcgat ttgatcacct cccctcgttt tgacgaggcc
    18961 tctctagaac tagagagagg agtcatttta gaggagatcg cctcggccga ggacgacccc
    19021 tacgacagaa tatatgacat gaccattgag aaggtttggg accgtgattt cggcaggccg
    19081 attctcggct atcaggatac agt
//
LOCUS       DAOQYI010000031        17720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_3577_length_17720_cov_77.157883, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000031 DAOQYI010000000
VERSION     DAOQYI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 17720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17720
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3577_length_17720_cov_77.157883"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..673)
                     /locus_tag="PKL93_08140"
     CDS             complement(233..673)
                     /locus_tag="PKL93_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71265.1"
                     /translation="MKKGIFLAIVGAAMIIAGLFLPIVRFPGGGEATYFNGGDLYGAL
                     TMAIAIFAGIMGIVKMRSMVFLSGLASIGVVVARLITVIRDLPDENSLRTQFKALIQS
                     NVPGSSGEEIANIIGGPQLHWLGWTILFAGGVVVLFAAFISSKE"
     gene            complement(768..1154)
                     /locus_tag="PKL93_08145"
     CDS             complement(768..1154)
                     /locus_tag="PKL93_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71266.1"
                     /translation="MQSIFLPLNWNTAEKTRGFTSLFRAEFHLPNYGWIQVDASVGQG
                     VMYSVGVTEEESRIYKDFYFGNFDPLRMVIQNSVDTIPEERPKEVQSLEKVMQLPFFE
                     SEFGNENLGVNDLITDSFTIRALLMR"
     gene            complement(1214..1591)
                     /locus_tag="PKL93_08150"
     CDS             complement(1214..1591)
                     /locus_tag="PKL93_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71267.1"
                     /translation="MIEIIGIVPVENLVDYPRTDGKIACTLLSYNLGSLEEALSIQIV
                     FSFRHFRQEFDIDPSQIGEYDIESPMYIESTLSTESIYYNEEMKDLAGRIVGEEKKPY
                     LQARLLYDFVVENTSTALLPKLL"
     gene            complement(1972..2121)
                     /locus_tag="PKL93_08155"
     CDS             complement(1972..2121)
                     /locus_tag="PKL93_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="HNQ71268.1"
                     /translation="MKRLITLLAFVLFATAAVIALEKSPDYYTSQKETKSIMEFTIHS
                     MMKEK"
     gene            2425..3033
                     /locus_tag="PKL93_08160"
     CDS             2425..3033
                     /locus_tag="PKL93_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNQ71269.1"
                     /translation="MKPKISLFLSVIFITFLISPLYANAIEPLARPPIIATAIGDGAA
                     SDVLALVCEIHGIDYENILQLKPEEFMEKLDSKNAPGTLAIAPGAMVEGDLYTLCGVE
                     EIDVGQEISRIEELISIAKARGVPVVAIHIEGGFTSPDNDPRQSFDLLMPKADYIILV
                     SPEGPSEYFSALSEQTGVQLIVVDKAERILDALDLLFSMSGS"
     gene            3377..3844
                     /locus_tag="PKL93_08165"
     CDS             3377..3844
                     /locus_tag="PKL93_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71270.1"
                     /translation="MDSEKLTEQEWHKKQAIVNFNLTWDLIDKKDRTAEDDLLMIHAA
                     HASRFHWGVVGTPIEFARGEWQVSRVYSLLNMAESALYHGELSLKYCTDNAISGFDLA
                     FGYEAVARAYMIAGKRDRMDEYMKLAREAADLIEDEQDKNYFLSELETIAPQQ"
     gene            complement(3932..5851)
                     /locus_tag="PKL93_08170"
     CDS             complement(3932..5851)
                     /locus_tag="PKL93_08170"
                     /inference="COORDINATES: protein motif:HMM:NF006938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HNQ71271.1"
                     /translation="MLEKRKSIATVLLILLAFSSLLFSATGKTVHNLVIMGTTDMHQY
                     IMPYDYMGDKPNESIGLAKVFTLIEKVRSANANTLLFDTGDFIQGSLVGDYEADVNPL
                     EGFDFQTIVRAYNYIGYDAVSVGNHDVTDFGLEFFERARSNSVFPWVSANIRMVEDPK
                     DFLVNPFVILEREVDGIPIRIGVIGFTPPQIMSWGRRHLEGTVFTQTIIEQAEKYIRV
                     LKDQSDLVVAVAHTGISTDEITSYDAQENAAYYLAQVEGIDAMILGHQHSHFPGDFAD
                     IEGIDNDKGLIFGVPTVQPGSWGSHLGIINLDLAYDWTTGDWEVLDGSASLVPVTPEV
                     ETHRQLAELVAQKHEATIEYVRTPIGWTDTEITSYFSRIIDNPVTQIINEAQIWWAER
                     EFAGSEYEWLPVLSAAAPFIAGRQGPTYFTHVQGDITIGSITDIYIYPNTIYVAKLNG
                     EQIKDWLEASANNFNQIDPYSTEPQHIVNYDFREYNFDVIEGIDYVYDISRPAGQRLI
                     SATFEGNPLDASMEFLVVTNNYRGSGGGNFPHVADNVILATTEINREAIIKYIQYIGE
                     IDPVPTNNWKILPIDTAGPLLYRSSPEGVGYIERNAIRGIEFIEVDEAGWGIYEVDLV
                     ELAKYLEDTVMQEVK"
     gene            6404..6844
                     /locus_tag="PKL93_08175"
     CDS             6404..6844
                     /locus_tag="PKL93_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNQ71272.1"
                     /translation="MKLNLLKRVFFSLSMETDRENILSALFESIAFDISMISDEIKRT
                     ALEPGQICASGGGTRSEVLLGLIATVLDRELLVCPQEYDSASGAGLITLSAFSESSLS
                     EVVDKIKERYVIVEPWNEFREYIFERKEAFKELYNCNRELFLKR"
     gene            7267..7470
                     /locus_tag="PKL93_08180"
     CDS             7267..7470
                     /locus_tag="PKL93_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71273.1"
                     /translation="MIYYVDDNAKPTGEHEIHTEYCDRLKELKSKTRLGDFVNCFGAL
                     QKAREIYPNVDGCIHCCSECHKG"
     gene            7738..8631
                     /locus_tag="PKL93_08185"
     CDS             7738..8631
                     /locus_tag="PKL93_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNQ71274.1"
                     /translation="MDRVVIVGNVDVDIIAGPIDDWPEWGTEIGVDTVDMRIGGQAAN
                     TAVVLADLGVPVEIVTVAGNDLFGKALERRFIEMGISVEGLDLIEGKTPHTVAITHKS
                     SERSFLSDESVMRFMDDDFVSNKLTNVKGAHILFCGINVLNGLRRGDLRVLLESLNDD
                     NVIYMDPGWPPDGWKSFRPQIRSLLNSLDWFLPNEAELIAVTQETSTSSAVESFQRDF
                     RSNAMVKMGSEGSLMISRESSEEYSTQRSENVVDTIGAGDSFNAGFIYSAAVKGSYDV
                     DLAHRIARDWIEGRYREKISL"
     gene            8692..9327
                     /locus_tag="PKL93_08190"
     CDS             8692..9327
                     /locus_tag="PKL93_08190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ71275.1"
                     /translation="MKRVFLREGQRVLFQGDSVTDTDRDREDFFSLGDGYPAIISGLV
                     GSAHPELRIEFLNRGVGGDRVRDLRRRWEVDCLDLSPDWVSILVGINDCWRLYDSNDK
                     TEPEDFENDYRYLLESVRSKGASVIIMEPFFLPVFSGMERWTEDLGPKIQIIRRVARE
                     FGAIYVPLDGIFAAASARCEPEFWAEDGVHPTPEGHALIARHWLQAMNITL"
     gene            complement(9343..9891)
                     /locus_tag="PKL93_08195"
     CDS             complement(9343..9891)
                     /locus_tag="PKL93_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNQ71276.1"
                     /translation="MTCEVLLLGNPLLRETSSPVSDFRSQETIGQIVMLKQALDEFRK
                     ENGFGRGISAPQIGILKRIVALNLGQGSFVIANPRIVDRSRATFTMWDDCMSFPHLLI
                     RLERSLSVDVVYEDERGIEHEWKGVDQARSELLQHEIDHLDGILAIDHALDAKSIIYR
                     SEYERNREYYDHLVDYSIEPTI"
     gene            complement(9881..10510)
                     /locus_tag="PKL93_08200"
     CDS             complement(9881..10510)
                     /locus_tag="PKL93_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ71277.1"
                     /translation="MNINSLVPDNSEKGTGLIIKHRGLYLFQVSGRKHRTEFGERFFA
                     GIGGHCEEGEGFIQAAKREAVEETGKAVEIIHSVRTDIVQSDGSVVDTIELTSPAPRM
                     IYEMIDRRKDLPEKEPYFIACFNAHFIDDTPFELDPEEVSALIAIPEELLCESLERKI
                     ALEDIISSGGEVVADSLETGTLLFPLGTAAALAHLLIRAKDLSEVDDDV"
     gene            complement(10625..12241)
                     /locus_tag="PKL93_08205"
     CDS             complement(10625..12241)
                     /locus_tag="PKL93_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ71278.1"
                     /translation="MAEIYDAIIVGGGIAGMTSAAYIAKHGAKVLLLEKNEKVGGLIN
                     SFWRDGFLFDGGVRALESAGIILPMLRELGIEIERVKSPVSVGIEDSVIRVTSKESLK
                     EYSDLLRKMYPGSEDEIESVVSFIKRIMKDMEILYGVDNPLFMDFKNNKSYFVKVYLP
                     WLFKFIFTLRRIGKFRMPVEEFLEKIVQNSSLRDIIDQHFFKNTPTFFAMSYFYLYQD
                     YFYPKGGVGTMAERVREKIVQFGGEIETGREVTEIHAGKKVIVDSNGRSNKYRKLVWA
                     ADLKKLYSIVKTDGLHETATETVKAFRSEISKKHGTDSIFSVFIAVDEAPEKFEAISS
                     GHFFYTPSKKGLGETHRSEMKSILENWSVNSKEEVLSWLDGFCKLSTYEISIPVLKDR
                     DLAPQGKTGLIVSTLFEYEIVKKAYESGWYGELKEELEKRIIEVLSNSIYPILKDRIL
                     FSFSSSPLSIESYSGSSEGSIVGWSFEEPIPVVASMMKVNSSVKTKIPDVLQAGQWAY
                     SPAGVPISILTGKLAADSVTKNLNRDRSAG"
     gene            complement(12225..13196)
                     /locus_tag="PKL93_08210"
     CDS             complement(12225..13196)
                     /locus_tag="PKL93_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ71279.1"
                     /translation="MERGKSPIRKYWKQYKWSNIRAMMKNNKSDPKICNDDFEGRLMV
                     ITGGTSGIGYYTCRKYASHGARILSINRNREKSESLCDELKHDFGVQCSYMIADFSSL
                     DDSLRVGKELSATGDTIDVLIHNAGVFLKRKELTKEGFETTFVVNYLSSFVINYLIRE
                     KMKAQGRGRILMVNSEGHRFAVWGIETEDLNWQKRRYSGLKAYGSAKTCQLLSILILD
                     QYFKGSGVTINAMHPGAVKTGTGKENGAFYKWYKRNVVDRFSRTPEISAEALYYLGVS
                     GDLQDVGGKFFNLTTEEIPAPPALDMETAKKLWQESLRMGAIDGGNL"
     gene            complement(13206..13649)
                     /locus_tag="PKL93_08215"
     CDS             complement(13206..13649)
                     /locus_tag="PKL93_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNQ71280.1"
                     /translation="MDGFCIESADQIRELWSKTYNTEGKPDWSHILPYYDDNIFFKDT
                     IQEIRGIEEFKKMTERLAERSKELKMAVLRVIKEDNVVFVEWEMTILFKKTRTSVIYG
                     ASRLSLSEEGKIIEQRDYYDLWGDIFDNIPSFRKPYRRFMRKHFG"
     gene            complement(13877..14734)
                     /gene="lipA"
                     /locus_tag="PKL93_08220"
     CDS             complement(13877..14734)
                     /gene="lipA"
                     /locus_tag="PKL93_08220"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNQ71281.1"
                     /translation="MKEKPDWLKKKLNADRSKLSEIKVMLRSLRLHTVCEEARCPNIG
                     ECFASRTATFMILGNICTRNCRFCAVSKGIPGSPDPKEPENISHAVHLLQLRHAVITS
                     VTRDDLNDGGASQFVDVVRELRKNCPDTTIELLIPDLNGNWKALERIVREHPDVLNHN
                     IETVPSLYANVRPGATYERSNGLLRRVKEIDSSIITKSGIMVGLGEGEDEVKSVIDDL
                     IGAGSRMLTIGQYLQPSHKHLPVVEYITPEQFERYRIFALERGFSFVASGPLVRSSYH
                     AALGFSSLH"
     gene            complement(14709..15416)
                     /gene="lipB"
                     /locus_tag="PKL93_08225"
     CDS             complement(14709..15416)
                     /gene="lipB"
                     /locus_tag="PKL93_08225"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNQ71282.1"
                     /translation="MENRSCFSLRLDGLTKYLEGLNLQNLALEKVMSGKADCILILLE
                     HEPVLTVGKSGGRENLLVDERELERLGVELRNTGRGGNITYHGPGQVVAYPVLNLGKW
                     KKDLPWYVHSLEEVVIRVLEDYGIEAGRKPEYRGVWVKETKIAAVGIAVKRWFTMHGF
                     ALNVRVNKEHFKLINPCGIKDYTIASMDDFVDSVDLHEIIESVEEKFSLVFKSKLIEV
                     SREWLESGQSEGETRLA"
     gene            complement(15419..16537)
                     /locus_tag="PKL93_08230"
     CDS             complement(15419..16537)
                     /locus_tag="PKL93_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNQ71283.1"
                     /translation="MYADNDSELFFGAGYATAGDRTFQMELSRRKVAGTLSEIYGKDL
                     LESDKLMRTLGLYKHAQELPDVQNPFSGFVARACHLSVGDWYPYSLTIGTGGTGHNPI
                     SMRLYEPLDDQNEFTFESFSEIHRDNVSAIARDFLSHAGILSEKNLLSQNSTRLLKAP
                     SDWDYYRLENIRAADVAETIVQVMHRSLRPNTVTALLASKYGGGHAGNIFLLRSALEE
                     FERTQKLPEEEELAVWVNQLLEASAGSLREAVSQVRKVVMVYQTNLEGFPSIYPDANR
                     KVPNLVTTSGNTIWSQLGNSYTQIVDLSNIDKSRAFLTPGASEDPRSPHFFDQVELWV
                     TGETRPAPLSREEVMKYVESIETLFSPINKTMRQSGSD"
     gene            17026..17148
                     /locus_tag="PKL93_08235"
     CDS             17026..17148
                     /locus_tag="PKL93_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71284.1"
                     /translation="MRLGLGEEGDEINFLFRTAFFENDEDRESYVSDPPIFIPS"
     gene            complement(17376..>17720)
                     /locus_tag="PKL93_08240"
     CDS             complement(17376..>17720)
                     /locus_tag="PKL93_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ71285.1"
                     /translation="CATKENLLDVIKAKLADHGIDLQNDDIKPPHHVPGDSEFVKTLL
                     RCYESYTGEKGYCISMGGGTYVHRLKNGVAFGCTMPGSENNMHGPDEFAVIEDLVTSA
                     KIFTQSIIELCG"
BASE COUNT         4599 a         4131 c         3945 g         5045 t
ORIGIN      
        1 tttgggcatt tgtccgttcg aatcatcgaa tacctccagt catcatctag aatcgaaatg
       61 tttctatcct ctattagaag ttacaaaaaa attgggagtc ttggtttttt ttctgccgcc
      121 atttgactct tcaaccttgc tgatagttcg ctcaccatga gtattgcagt catgtcatgc
      181 gcgtcgtata gatatcattt gaaaatccta accaaggccg gaggtaagcc cattattctt
      241 ttgaagaaat gaaggcagcg aagagaacca caactcctcc tgcaaatagg attgtccacc
      301 caagccagtg aagttgcggt ccaccgatga tattcgctat ttcctctcca gaactaccgg
      361 gaacattgct ctgaataagc gctttaaact gtgttctcaa agaattctcg tcgggcaggt
      421 ccctaatcac ggtaatcaac ctcgctacta caacgccaat cgaagcaagt ccggaaagaa
      481 agaccataga tctcattttc acgattccca tgatacccgc aaagatagca attgccattg
      541 ttagtgcgcc atatagatct ccgccgttga aataggttgc ttcgcctccc cctgggaaac
      601 gtactatcgg caggaacaaa ccagctataa tcattgccgc tccgactatg gcgagaaaaa
      661 tccccttttt cataatctcc acctcttttc taatatccta cgagcataag actttaatct
      721 agtctaggcc aatgatatca gaataccact tcagggtgtt gtatgtttta tctcatcaag
      781 agagctctga tcgtaaaact gtcggttatc agatcgttaa ctccaaggtt ttcatttccg
      841 aattcgctct cgaaaaatgg gagctgcatt accttttcca gagactgaac ttccttcggt
      901 ctttcttctg gtatcgtgtc aactgaattt tgaattacca ttctcagcgg atcgaagttc
      961 ccgaagtaga aatccttata gattctgctc tcttcctcag tcactccgac agagtacatt
     1021 acaccctgtc ctactgacgc atctacttga atccacccgt aattggggag atgaaattca
     1081 gcccggaaaa gagaggtgaa cccccgggtt ttctcggcgg tattccaatt gagcggcaga
     1141 aaaattgatt gcatttcata atctccatat ccgtgttcca tagcaaaaag gctttctgga
     1201 ataccttccg cctctagaga agcttgggca ataaggctgt agacgtattc tctacgacaa
     1261 aatcgtaaag gagtctggcc tgaagatatg gttttttctc ttctccgacg atccttccgg
     1321 caaggtcctt catctcttcg ttgtaataaa ttgattccgt agaaagggta ctctctatgt
     1381 acatagggct ttctatgtca tattctccga tttgagaagg atcgatatcg aattcctgcc
     1441 tgaaatgcct gaaagagaag actatctgta tggacagagc ttcctccaga gaacctagat
     1501 tataagaaag aagcgtgcag gcaatcttcc catcggtcct aggatagtcg acaaggtttt
     1561 cgaccgggac tatgccaatt atctcgatca tgtcctgtcc taacctgttc agctgaaggg
     1621 gaagcaagat ctttagattg tcttcatccg gaagatctga ctttgaaacc tcgaccgagt
     1681 attcgaccag aaactctctg gcatagaaga aaccgcttca gtggcgtttg aactcgacaa
     1741 agcccgcctg tctcttccgc ccatattatg aaagaacgaa ttttacagct tcggtgaagt
     1801 gatcggccca atctggattc tgctccacta gatcgagatc cctgtgaaaa gatttctttc
     1861 aaagcctgtg aagaatatct cggcagtcca atatctttct ttgaatgtct ctttcttcaa
     1921 cgaaatcata gagcttctca tgtgaatcta tctgaggata gttactcttg attatttctc
     1981 tttcatcatc gaatggatag tgaattccat tatggatttg gtttctttct gtgatgtata
     2041 gtaatccggg ctcttttcaa gtgctattac cgccgccgtc gcaaacaata caaatgccag
     2101 taatgtaatc agtcttttca taagaacctc cttgcgaacc gtcaataaaa ccgataaaaa
     2161 atatgttaat tatataattt atgtattttg tagcgaagac atctcaccta tccatattgt
     2221 acggattctt gctgcttctt ttagaagttt gaactctgct tgagaaggtc ataatcagga
     2281 tttcaagcga agaaagctgt ccttgtgcag ataagagtta aatttgataa aattagtcat
     2341 attaaattct ttgttgtttg catgagaagg acagtttcta tgctgcatct tgactctgcc
     2401 atcgaagatt gtggagggga atacatgaaa ccaaagatat cattgttttt atcggtcata
     2461 ttcatcacgt ttctaatctc gccgctttac gcaaacgcta tcgagcctct tgcaagaccc
     2521 ccgattattg caacggcgat aggcgacggt gctgcatcag acgttctcgc tctagtctgt
     2581 gaaatccacg gaattgatta cgaaaacatc cttcaattga aacccgaaga gttcatggag
     2641 aagctcgatt cgaaaaatgc gcccggaact cttgctattg cccctggtgc catggtcgag
     2701 ggggatctat ataccttatg cggtgttgag gaaatcgatg tagggcagga gatttccaga
     2761 atagaggagc ttatatcgat tgcgaaagcg cgaggagttc ccgtcgtggc aattcatatt
     2821 gaaggtggct tcacttcacc agataacgat ccgaggcaat cattcgattt gcttatgcca
     2881 aaagccgact acataatcct ggtgtcaccg gaagggccga gtgagtattt ctccgcgctc
     2941 tcggagcaaa ccggggtaca gctgatagtg gtcgacaaag ctgaaagaat tctcgatgct
     3001 cttgatctct tattctcaat gagtggatct tgatgtagtg ctttaaacat agtctcactg
     3061 gatcagtttg aaatttcttt gatgatctct gaatagttct atagttctca taaatcagtc
     3121 ttcttaaggg attgagttac agaatgggat taaggattcc gtgttgttca atattggagc
     3181 atgcaaatca ccctcgagat ttttctcttg cacaataagg gctttccaat tcgccgaatg
     3241 gttattgcta ctaatctagc ctcttcaggt accttgaatt cttagtgagt cgctccagct
     3301 aattgctctc cttgttacca gtgatagaat tgggatgcgg gaggaagaca gaattatcct
     3361 ggaaaggatg gttgttatgg attccgaaaa gcttacggag caggaatggc acaaaaagca
     3421 ggcgatagtg aacttcaacc ttacatggga cctaatagat aagaaagaca gaactgcaga
     3481 agatgacctt cttatgatac atgccgctca cgcatctcgc ttccactggg gagtagtagg
     3541 taccccgatc gaatttgcaa ggggtgagtg gcaggtgtcc agagtttact ctttactcaa
     3601 catggcagag tcggccttgt atcacggaga gctttcactt aagtattgca ccgataacgc
     3661 gataagtggt ttcgatcttg cctttggcta tgaagccgtt gcaagagcct acatgatcgc
     3721 aggaaaacgc gaccgtatgg atgagtatat gaaactggca agagaagcag ccgatctgat
     3781 cgaggatgag caggacaaaa actactttct ttcagagttg gagaccatcg ctcctcaaca
     3841 atagtcacag ggcttcgtaa ctgtagtttc agatcgaaat gacagaaaag aaattgcggg
     3901 ccccgtaagg ggcccgtttt ctaaaagtaa gctatttaac ttcttgcatt accgtatctt
     3961 caaggtattt agcgagttct accaaatcaa cttcatagat tccccatccc gcctcatcga
     4021 cttcaatgaa ctctattcct cgtattgcat tcctctcgat gtatcccacg ccttcgggag
     4081 aagaacggta cagaagtggc ccggccgtgt caatcggcaa gattttccag ttgttcgtcg
     4141 gaactggatc aatttcacct atgtactgta tgtacttgat tatggcttca cgatttatct
     4201 cggttgtcgc cagaattacg ttgtccgcca catgtgggaa atttcctcct ccgctccctc
     4261 tgtagttgtt tgtcactacc aagaactcca ttgacgcatc aagcggattg ccttcaaagg
     4321 tcgcagaaat caggcgctgg ccagcaggcc tgcttatgtc gtaaacataa tcgattccct
     4381 cgattacgtc aaagttgtat tccctaaaat cgtagttgac aatatgctgc ggttctgtgg
     4441 aatatgggtc aatctggttg aagttgtttg ccgacgcctc gagccagtct tttatctgtt
     4501 ctccgttgag ttttgccaca tagatagtgt tcggatagat ataaatgtcc gttatcgagc
     4561 cgatcgttat gtctccttga acgtgtgtga aatatgtcgg accctgcctg ccggcaatga
     4621 aaggtgctgc agcactaaga acaggaagcc actcgtattc agatccagcg aattcacgtt
     4681 ctgcccacca tatttgcgcc tcattgatga tctgtgtgac tgggttatca atgattcttg
     4741 agaaatagga agttatctcg gtgtcggtcc agcctattgg agtccttaca tactcaatag
     4801 ttgcttcatg tttttgagca accagctcag caagctgccg atgagtctca acttcaggag
     4861 ttacaggaac aagcgaagct gaaccatcta gtacttccca gtctccagta gtccagtcat
     4921 aagctagatc gagattaatg atacctaaat gcgaacccca ggaaccgggt tgtacagtcg
     4981 gaacaccaaa gatcagcccc ttgtcgttat cgatgccttc gatatctgcg aaatcgccag
     5041 ggaaatggga atgctgatga ccgagaatca ttgcatcgat tccttcgacc tgagcgaggt
     5101 aataagcagc attctcctga gcatcatagc ttgttatttc atctgtggat attccggtat
     5161 gagcaactgc aacaacgaga tccgactgat ctttaagaac acgaatgtat ttctccgcct
     5221 gttctataat tgtctgggtg aacacggttc cttctagatg acgccttccc cagctcatta
     5281 tctgtggagg tgtgaagcct atcacaccta tcctaattgg gattccgtcc acttcccttt
     5341 caagaataac gaagggattc accaaaaaat cctttggatc ttcgaccatt ctgatatttg
     5401 cggaaaccca ggggaagaca gagttacttc ttgcacgttc aaaaaactcg agaccaaagt
     5461 cagtgacatc atgattgcct accgaaacgg catcatagcc aatataatta taagcccgca
     5521 cgattgtctg aaaatcaaag ccttcaagag gattaacatc ggcctcgtag tctcctacta
     5581 gacttccctg gataaagtcg cccgtatcga acagcagagt gtttgcgttt gcgcttctga
     5641 ccttttcaat gagtgtgaaa acctttgcga gaccaatact ctcattcggc ttatccccca
     5701 tgtagtcgta gggcataatg tactgatgca tgtctgtcgt acccataata acgagattgt
     5761 gaacagtctt gccagttgca gagaataaca aacttgaaaa ggcaagtaag atcaagagaa
     5821 cagtcgcgat actctttctt ttctcaagca ttttctcact cctccttgaa agagacaggt
     5881 tgcttttcat tcaggaacga agcaaccatg tgggtattta ttatataact ggcatcatct
     5941 accagcaaag aaatacttag tgtgttctca gataatacat ctactttttg aagagggttt
     6001 tcgataaacg tgaatggatc ttgctccgga tctctagaat ttacatgagg acaaagaaag
     6061 aagcggtcag agtcttccgg catataagta ctcttgaatt ctttggcaaa ctcaacagag
     6121 tttcatctgt aatacccagt taggtctctt ggagacatca ctgaatatta gattcacagt
     6181 gagaatggga ccttaagtca ctgaggaact tgggtactcg ttgatccaaa tgacttcttg
     6241 agcttcgtta atgattcagt tcttctgcag caagaattct gatgaatttg ctgccgctct
     6301 gggtcgacga acggttattg gcctcgagat cttcaatatc tctttgcccc aataatcaag
     6361 agttgcaaaa gcctaccatg taccgattcc tacggcgtaa agcattaaat taaatctact
     6421 caagagagtc ttctttagcc tttctatgga aacggacagg gagaatattc tctctgcgtt
     6481 gtttgagtct attgcatttg atatcagcat gataagcgat gagatcaaga ggacggcact
     6541 tgaaccaggc caaatctgtg cctcaggagg aggaaccagg agcgaggttc tcctcgggtt
     6601 gattgccact gttctagatc gtgaactcct ggtttgtccg caagaatacg actctgctag
     6661 cggtgccggg ctcattactt tgtcggcgtt ttcggaatcg agcctctcgg aagtcgtaga
     6721 caaaatcaaa gagagatatg tcattgttga accctggaat gaattcagag aatacatttt
     6781 cgagaggaag gaagccttca aggagctgta caattgcaat cgagaactct ttttgaagag
     6841 atgaagtttc tttgagaaca gaggaagcat cttagagatg aaaaaatcag ccggggaaac
     6901 ccccggcttg attatatact aagaaattaa caggaacgag cgcttcacag aaagattgcg
     6961 ctagttttct gtatattata agtctttaga agcaaagtct tgcaagggag ttagcggtca
     7021 tgaacttggg atttgctctt atcttaattg gcttggaatt tgcaggatct cgcttcaaaa
     7081 aaggttgaaa gaatatcgcc cattcaggat gcatagatcc gaaagattcg gaaacgacgt
     7141 cttaaatcta cgtggacctg atctcattct tgcgaagagc acgaagtgct ataattttgg
     7201 tgtgagacgt tctagataaa ctctttcttc ggctatgaag tatttgtttg aaggaggtgt
     7261 tgtgaagtga tttactacgt agatgacaac gcaaagccga ccggagaaca tgaaattcat
     7321 acggagtact gcgataggct caaagagtta aaatcgaaga caaggctggg agatttcgtt
     7381 aactgcttcg gagcccttca gaaggcgaga gagatatacc caaatgtcga tggctgcatt
     7441 cattgctgca gtgaatgcca taagggctga agcgtcttca taaatctcag attaatggtc
     7501 gcttgtaggg aataaatgaa tctttcttgc agattcgttg gctggcgttt ttgctttttc
     7561 tggtgatttc tccggccata gagcattttg ttaactttca cttttcagtc tcttttgtga
     7621 tgacggcctg caacggttaa ttacaatcca tttcagagtt ccgcattctt ttcggatgct
     7681 ataatcattc ggaactgatg attcacaccc gcggatatcg gagaagagag tggtgaaatg
     7741 gacagagtag ttatagttgg caacgttgat gtcgatatca tagcaggtcc gattgacgac
     7801 tggccagaat ggggaacgga gataggcgta gatactgttg atatgcgtat cggcgggcag
     7861 gcggccaaca cagctgttgt gctcgccgac ctcggagtac ctgtggaaat agttactgta
     7921 gccggaaatg atctcttcgg gaaggcctta gagcgaagat ttattgaaat gggaatttcc
     7981 gtcgaagggc ttgacctcat cgaaggcaag actcctcata cagttgcgat aactcataaa
     8041 agcagtgaga gaagcttctt gagcgacgaa tctgtcatgc gtttcatgga tgatgacttt
     8101 gttagcaata agcttactaa tgtcaaaggt gcacatatcc tgttttgtgg aatcaatgtt
     8161 cttaacggtt tgagaagggg agacttgagg gttcttctgg aatctctcaa tgatgataac
     8221 gttatctaca tggatccggg atggcctccc gacggctgga agtcattcag acctcagata
     8281 aggagcttgc tgaattcatt agactggttc ctacccaacg aagccgaact aattgccgtg
     8341 acacaggaaa cttcgacttc cagcgccgtt gaaagctttc agagggactt ccgtagtaat
     8401 gcgatggtga agatgggctc tgaaggttcg ctcatgatct ctagagagag ttcagaagag
     8461 tattcgaccc aaagatcaga gaacgttgtg gatacgattg gagccgggga ttccttcaat
     8521 gccggtttta tctattcggc ggccgtcaaa ggaagctatg atgtcgacct tgcccacagg
     8581 atcgccagag actggatcga gggaagatac agggaaaaga tttccttgta ggttgctctg
     8641 attagttttc gcagtttttt ggaagtatac tgtctattca taagcagggg gatgaagaga
     8701 gtgtttctta gagaaggtca aagagtactc tttcaagggg atagcgtgac tgatactgat
     8761 agagacagag aggacttctt ttctcttggg gacggttatc ctgcaattat ctccggactc
     8821 gtgggttctg cgcatccgga actgagaatc gagtttctga acagaggcgt tggaggagat
     8881 agggtacggg atcttcgcag acgctgggaa gttgattgcc ttgatctctc tcctgactgg
     8941 gtttcgatac ttgtgggtat aaacgactgt tggcgcctct acgacagcaa cgataaaacg
     9001 gagccggagg acttcgaaaa cgactacagg tatctgctgg aatcggtcag gtcaaaggga
     9061 gcatctgtga taataatgga acccttcttc cttccagtct tctcagggat ggagagatgg
     9121 actgaagatc ttggaccgaa gatccagata atcaggagag ttgcgaggga atttggagca
     9181 atttatgttc cactcgacgg aattttcgct gctgcatctg cgagatgcga gccggaattc
     9241 tgggccgaag acggtgttca tcccactccc gagggacacg ccctcattgc ccgtcactgg
     9301 cttcaagcaa tgaatatcac tctttgattc gggtagttaa cctcaaattg tcggctcgat
     9361 gctgtagtcg acaaggtgat cgtagtattc gcgattcctt tcatattccg agcgatatat
     9421 gatacttttg gcatctaatg cgtgatctat tgcgagaatg ccgtcgaggt ggtctatctc
     9481 atgctgaagc agctccgatc ttgcctgatc gacccccttc cattcgtgtt cgattcctct
     9541 ttcatcttca tacacgacat ctaccgaaag gcttctttcg agcctaatga gcagatgtgg
     9601 aaatgacatg cagtcatccc acattgtaaa tgtagcccta ctgcggtcga ctattcgcgg
     9661 gtttgcaatt acaaaacttc cttgacctag attgagcgca acgattctct tgagaatgcc
     9721 gatttgcggg gctgaaattc cgcgaccgaa gccattttct tttctgaatt catcaagggc
     9781 ctgtttgagc atgacgatct gccctatcgt ctcctggctt cgaaagtcag agacgggaga
     9841 ggatgtttcg cggagcaacg gatttccaag aagcaatact tcacacgtca tcatcaacct
     9901 ccgaaaggtc tttggctctt atcaagagat gtgcaagagc cgctgccgtt cctaggggaa
     9961 agagcaaagt tccggtctcc agagagtcgg cgacaacctc tccccctgag gagattatat
    10021 cttcaagtgc tatcttgcgc tcaagacttt cgcagagcaa ttcttccggt atggcaatta
    10081 aagcgctgac ctcttcaggg tcgagttcga agggagtgtc atctatgaaa tgcgcattaa
    10141 agcaggctat aaagtaaggc tctttctcgg gtaagtcctt tcgtcgatca atcatttcgt
    10201 agatcattct gggggcgggt gatgtaagct cgatcgtgtc aacaacgctt ccgtctgatt
    10261 gaactatatc cgtcctcact gaatgaatta tttcgacggc ctttccagtc tcttcgactg
    10321 cttcgcgttt tgctgcctgg atgaacccct cgccttcttc acagtggcca ccgattccgg
    10381 cgaagaacct ttctccgaac tctgttcgat gcttccttcc agaaacctga aagaggtaga
    10441 ggccccggtg ctttataatc agtccagtgc ccttttcgga attatctgga accaaactgt
    10501 tgatattcat agtattcttt ccttcattac tttattgtcg agacaatcat ctatttatcc
    10561 aaagaggcga tcttccactt gtccgaagac cactgtcgct ggtctacaga caacgtaatg
    10621 gtctttagcc ggcagatcga tccctattca aattctttgt aacggaatcg gcagccagct
    10681 ttcccgtaag gatgcttatc ggtacacctg cagggctata agcccactgg ccggcctgaa
    10741 gaacatccgg tattttcgtt ttaaccgaac tgtttacctt catcatactg gctactactg
    10801 gtatcggttc ctcgaaagac catcctacga tcgagccttc agagctgccg gagtaactct
    10861 ctattgaaag cgggctgctg gagaatgaaa agagaatcct gtctttcaaa atcgggtaga
    10921 ttgaattaga taaaacttcg attattctct tttctagctc ttctttcagc tctccgtacc
    10981 agccggattc atacgctttc ttcactatct cgtattcgaa gagtgtgctt acaataagac
    11041 cggtctttcc ttgtggggct aggtctctgt ccttgagaac ggggatcgaa atctcgtagg
    11101 tgctcagttt gcagaatccg tcaagccagc tcaagacttc ttcttttgaa ttgacagacc
    11161 agttctctag aatggacttc atttcgctgc gatgagtctc gccaaggcct ttctttgaag
    11221 gggtatagaa gaagtgcccg ctggagatcg cttcaaattt ctcaggcgct tcgtcgaccg
    11281 cgatgaaaac gctgaagata gaatcggtcc cgtgtttttt cgatatctct gatctgaatg
    11341 ctttgaccgt ttccgttgcg gtctcgtgca ggccgtctgt ttttacaatg gagtagagct
    11401 tctttaggtc ggcagcccac acgagtttcc tgtacttatt agatcttcca ttgctatcca
    11461 cgattacttt tttcccggca tggatctcgg tcacttccct acccgtctcg atttccccac
    11521 cgaactggac tatcttttct cttactcgtt ccgccatagt tccaactcca cccttcggat
    11581 agaagtaatc ctgataaagg tagaaataac tcatggcgaa aaaagtcggt gtgttcttga
    11641 agaagtgctg atcaatgatg tctcttaaag agctgttttg gacaatcttc tcgaggaatt
    11701 cctcgaccgg catcctgaat tttccaattc ttcggagggt gaaaatgaac ttgaacagcc
    11761 agggcaggta cactttgaca aaatacgact tgttgttctt gaagtccatg aagaggggat
    11821 tatccactcc atagagaatc tccatgtcct tcatgattct ctttatgaat gaaacgacac
    11881 tctcgatttc atcctcgctt ccaggataca tcttcctgag gagatctgaa tactccttca
    11941 aactttcctt cgaagtcaca cgtataacgc tgtcttcaat ccccacagat acaggactct
    12001 taactctttc gatttcaatc ccaagctccc tcagcatggg gagaataatt cccgcgcttt
    12061 cgagcgccct cacaccgccg tcgaaaagga agccatctct ccaaaaggag ttgatcaggc
    12121 cgccaacctt ctcgttcttc tccaacaaga ggactttagc tccgtgcttt gcaatatatg
    12181 cggcactggt cattccggct attccaccgc caactattat cgcgtcatag atttccgcca
    12241 tcgatggccc ccattcgtag gctttcctgc caaagttttt tagctgtttc catgtcgagt
    12301 gcgggagggg cgggaatctc ttccgtggtt agattgaaga actttccgcc gacatcctgg
    12361 agatcaccgg aaacaccgag atagtaaagg gcttctgccg agatttccgg cgtgcgggag
    12421 aatctgtcga caacatttct cttataccat ttgtagaacg ccccattttc ctttcccgta
    12481 cccgtcttga ctgcgccggg gtgcatagca tttattgtga ctccgcttcc tttgaagtat
    12541 tgatcaagaa taagtatgct aagcagctgg caagtctttg cagagccgta ggccttaagg
    12601 ccactgtaac gtctcttctg ccagttaagg tcctccgttt ctattcccca gacggcaaat
    12661 cgatggcctt ccgaattcac catcagaatg cggccccttc cctgagcttt catcttctct
    12721 cttattagat agttgatgac gaatgacgag agataattga ctacgaaagt cgtttcgaag
    12781 ccttcctttg taagttcttt gcgcttcaga aagactcccg cgttgtgtat caagacatct
    12841 atcgtatctc ccgtagcaga gagctctttg ccgactctca ggctatcgtc tagactgctg
    12901 aagtcagcta tcatatatga acattgaacg ccgaagtcgt gtttgagttc gtcgcaaaga
    12961 ctttccgatt tttccctgtt tcgatttatg gaaagaatcc tcgccccatg gctagcatac
    13021 tttctgcaag tgtagtatcc aatgcccgat gtgcctccgg taatgaccat taggcgaccc
    13081 tcaaaatcat cattgcagat ctttggatca gacttgttgt tcttcatcat cgcgcggatg
    13141 ttggaccatt tgtattgttt ccagtatttg cggatggggc tcttgcctct ctccatatgc
    13201 aggacctacc cgaagtgttt tctcatgaaa cgcctgtaag gctttctgaa tgagggtatg
    13261 ttgtcgaaga tatcacccca taggtcatag taatctctct gctcgattat ctttccctct
    13321 tcgctaagag agagacggct cgcaccataa atcacggaag tcctcgtctt cttgaagagt
    13381 atcgtcatct cccattcgac aaagacaacg ttgtcttcct taatgactct aagaacagcc
    13441 attttcagct ctttagatct ctcggcaagt ctctccgtca tcttcttgaa ttcttcgatt
    13501 ccccggattt cctggatcgt gtctttgaag aaaatgttgt cgtcgtagta tggaagaatg
    13561 tgggaccagt caggtttccc ttctgtgttg taagtctttg accatagttc tcgaatctga
    13621 tcggcactct ctatacaaaa tccatccata atcacaactc cccaaaacgt tcattagaaa
    13681 catacggagt cctgtattca ctcaggtaac tagtcaaaca tagagaatat cctaagcaaa
    13741 tcgtatcacg gcgactcttg cgaagcaaat atttggagga ttacaaaata gcaatcttca
    13801 gttctgcccg acaaactgtg aacgatagat ttgaaagtaa caaaagttca gaaacaatgc
    13861 tgttgaaaaa cgctctctag tgcaaacttg agaagccaag agcggcatga taggagctac
    13921 gaacgagagg accagatgcg acgaaggaaa agcctctctc cagagcgaag attctgtatc
    13981 tttcaaactg ctccggcgta atatactcca ctaccggaag gtgtttatga gatggctgca
    14041 gatactgacc aatagtcaac attctggacc ctgcgccaat caggtcatct ataacagact
    14101 taacctcgtc ttcgccctca ccaaggccca ccattatccc agactttgta attatcgaag
    14161 agtcaatttc cttcactctc ctcaagagac cgttagaacg ctcgtacgtt gctccgggtc
    14221 ttacattggc gtacagagaa ggtacggtct ctatattgtg attcaaaacg tccggatgct
    14281 ctctaacgat tctttcgaga gccttccagt taccatttag gtcaggaatc agtagctcga
    14341 tagtcgtatc tggacagttc tttctcagtt cacggactac atcgacgaat tgagaagcgc
    14401 caccatcgtt aagatcgtcc ctggtaaccg aggtgatcac agcatgtctc agttggagaa
    14461 gatggacggc atgggaaatg ttctccggct cctttggatc tggagagcct ggtattccct
    14521 ttgagactgc acagaatcga cagttccttg tgcagatatt tccaagtatc atgaatgtag
    14581 cggttctgga tgcaaaacac tctcctatat ttggacatct cgcctcttcg cacacggtgt
    14641 gaagacgcag gcttcgcaac atgaccttta tttcgctaag cttactccgg tcggcattga
    14701 gcttcttctt aagccagtct ggtttctcct tcactttgac cactctccag ccattctctc
    14761 gaaacttcta tcagtttgct cttaaagaca agagaaaatt tttcttccac actctctatg
    14821 atctcatgta aatcaacact atccacaaaa tcgtccatcg atgcaatcgt atagtctttt
    14881 atgccgcagg gattgatcag tttgaaatgt tccttattta cccgtacgtt cagggcaaat
    14941 ccatgcattg tgaaccatct ttttacggcg ataccgacgg cggcaatctt tgtttctttt
    15001 acccagacgc ccctgtattc cggtttcctt ccggcttcaa tgccataatc ttccagcact
    15061 ctaattacta cctcttcgag agagtgcacg taccatggaa gatctttctt ccatttccca
    15121 aggttcagaa cgggatatgc aacaacctgt cccggaccgt gataagttat gtttcctccc
    15181 ctaccggtat tacggagctc aacccccagg cgctcgagct ctcgttcatc tacgagaaga
    15241 ttctctcttc cgcccgattt gcccacggta aggactggtt catgttcaag gagaatcagt
    15301 atgcaatctg ccttccccga cataaccttt tcaagagcta gattctgcag attcaaccct
    15361 tcaaggtatt ttgtgagccc gtccagcctc agtgaaaagc aacttcgatt ctccattttc
    15421 agtcggaccc cgattgacgc atagtcttat tgattggcga aaaaagtgtt tcaatcgatt
    15481 ctacatattt catgacttct tctcgactca gaggagcggg tctggtttct ccggtaaccc
    15541 atagttcgac ttgatcgaag aaatgagggc tgcgtggatc ttctgaagct cctggtgtca
    15601 gaaacgctct ggacttgtct atgtttgaaa ggtcgacgat ctgtgtgtaa gagtttccca
    15661 gttgcgacca gatggtattt cccgaggtgg ttacgagatt gggcactttt cgattcgcat
    15721 ctgggtaaat cgaggggaag ccttcaagat tagtctgata aaccataacc acctttctaa
    15781 cttgagaaac ggcctctcga agactgccag ccgaagcctc aagtaactgg ttgacccaga
    15841 ctgccaattc ttcctcttcc ggcaacttct gagttctttc aaactcttcc agggcagaac
    15901 gaagtaggaa gatatttccg gcatggcctc cgccgtattt cgaggcaagg agagcagtga
    15961 ccgtattggg ccgcagactg cgatgcatta cctgcactat agtctcggca acatcggccg
    16021 ctctgatgtt ttccaaacgg taatagtccc aatcactcgg ggctttcaga agcctcgttg
    16081 aattctgtga cagaaggttc ttctcagaca aaatgcctgc gtgagataga aaatctctgg
    16141 caattgccga gacattgtct cgatgaatct ccgaaaagct ctcaaaagtg aattcgtttt
    16201 gatcatcaag gggctcgtaa agcctcatac ttattgggtt atggcctgtt cccccggttc
    16261 ctatagttag agaatacggg taccagtctc cgacggacaa atgacatgct ctggcaacaa
    16321 atcccgaaaa aggattctga acatcaggaa gctcctgcgc gtgtttgtag agaccaagtg
    16381 tgcgcatgag tttgtcggac tcgagcaaat ccttgccgta aatctcggat agcgttccgg
    16441 caacttttct ccttgaaagt tccatttgaa acgtacgatc ccccgcagtg gcatagcccg
    16501 ccccgaaaaa tagttcagag tcattgtccg catagatgtg agctattccc cattaatcac
    16561 gtactatctc gatttgacta ccaaatattc ttgcaggaag tatcgaaaga agcacaaaga
    16621 tagagatcac aaccatccac gatgcggttt gcttcgtcat agccgccccc ttttgctcac
    16681 tgtttggagt gaccctccga ttatagatca ctctactgat gggaggaact tcaagttaca
    16741 atccgtgatc caagcgatag tgaatatatg aagtgtcttt cagaaatcag ctatcagatg
    16801 agcctccata attggagatc tgatttcgaa acgtaaacaa tgattccaag gtatctgtga
    16861 agaagaaaaa cacatgaaag taatgcagtt attgaagcgc tggatgtcaa taactcactt
    16921 ttgctactga aggcttctct aaaacggtag tgttacctgg cagaattcag atcgtatctc
    16981 caatttgcag gccaaagaaa aacggagagg tttccctctc cgctattgag actgggtctt
    17041 ggtgaagaag gagacgagat taacttcctt ttcaggacgg ccttcttcga aaacgatgaa
    17101 gacagggaat cttacgtaag tgatccgccc atattcatcc cttcttagtc tactattcct
    17161 caactgcttg tccaattccc ttgggggggg tgaatacgac tcagaacttg gaaccaatgc
    17221 tctgctcttt caagcgttcg gtgcacagtt tttttcaagg atcagcgggt tctatgcttg
    17281 caacttggaa gttacaactt gcaactggtc ctttcggcga acggagatcc cataaaggac
    17341 aaccatggtc gctgctagct tacagaacga ctccttcaac cgcaaagctc tatgattgac
    17401 tgagtgaata tcttggcgct cgtcacgaga tcttcaatga ccgcaaattc atcggggccg
    17461 tgcatattgt tttcgcttcc gggcatagta cagccaaatg ccacaccgtt cttcagtcga
    17521 tgtacatacg ttccgccgcc cattgaaatg cagtacccct tctctccggt gtagctttca
    17581 taacatctca gaagtgtctt gacgaactcg gagtcacccg gtacgtgatg aggaggtttt
    17641 atgtcatcgt tttgcagatc tatgccatga tcggccagtt tggccttaat tacatcaagg
    17701 aggttctcct tcgttgcaca
//
LOCUS       DAOQYI010000032        17445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_3646_length_17445_cov_80.427142, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000032 DAOQYI010000000
VERSION     DAOQYI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 17445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17445
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3646_length_17445_cov_80.427142"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKL93_08245"
     CDS             <1..447
                     /locus_tag="PKL93_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNQ71286.1"
                     /translation="AQCSEDIQLKKLFEMLANDEQRHYEIISGFKDKNYNYKGTYTFK
                     TTRNMFSEMLNDKKCFEIDATNLEAYEHAVEMEKESVKLYLDQAKQTSEPSEKEILLK
                     LAAEENKHQIILENLMDFVRKGLDWTESPEFSHLEEWDKFTDLDKY"
     gene            complement(608..1327)
                     /locus_tag="PKL93_08250"
     CDS             complement(608..1327)
                     /locus_tag="PKL93_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ71287.1"
                     /translation="MLVPVILAAGKGKRLKSEVPKPLVKIKGKPMIIHILNKVSAFCE
                     SKCSIVVINPDFEKEFREVLGENTLLAYQDSPKGTADALKRSLHLIPDNSDILVMYSD
                     LVLIAKESLKSLVELHRNGDCDITFLSGITQEKFPYALVERNENGKVVSFEERKIPDF
                     PPPWEFYIGPIIIKKEIVQEYIGQLVPNKETGEIYIADIVALALSDNKSVCGFSTSNK
                     EEFLGVNTPEDLQTAEKLLTD"
     gene            1573..2217
                     /locus_tag="PKL93_08255"
     CDS             1573..2217
                     /locus_tag="PKL93_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNQ71288.1"
                     /translation="MEYLDLSLRLFCALVAGALIGATRERIYKPAGLRTHSLICVGAA
                     FITVLSTTVFVTAEHGDPGRVAAQIVSGIGFLGAGTILKKGFSVKGLTTAATLWVTAA
                     VGMGFGSGEYLLSVVVTVFALLIVLFLKRIELFIGGRNLPKVLIVAENSQETSRKVSE
                     WFKDNGLTVESMEIEEDDEVLSLLVEVSKGDAIKVKNIMVGLSTLKGVKSAELR"
     gene            complement(2221..2553)
                     /locus_tag="PKL93_08260"
     CDS             complement(2221..2553)
                     /locus_tag="PKL93_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71289.1"
                     /translation="MMTVTNGGLAGHSGKDVNLNNITVSFKFPVKPSALILYYGEYGG
                     NINVEINGILENVQDFSDIDGKVIGGVTVTLTSVSGPKGVLNLQGRITSFSIGGQELW
                     IDHICPRK"
     gene            complement(2550..2753)
                     /locus_tag="PKL93_08265"
     CDS             complement(2550..2753)
                     /locus_tag="PKL93_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71290.1"
                     /translation="MKRIVVFLLVAVCIFVISGCYLVLNCVDFEGSTVGTKYYNGDTL
                     FEQTTRMDMKPFVWSNGNRLPMV"
     gene            3273..3806
                     /locus_tag="PKL93_08270"
     CDS             3273..3806
                     /locus_tag="PKL93_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HNQ71291.1"
                     /translation="MFSKRVVFLPVDERFCTRDYFLLLCEAAGGVLEKGSDEIPLIVN
                     NPTDKLNAEDQPENQGKSTSFERVFTLLKRFVSEVTVIADVKYSNGSENKIIEAIMEI
                     HNFDWNSTNYSGWNTAGNTIGTVSAHSVVQLLVMKNFLSLDKVKMKHLQIIIFLEHWA
                     YKSKIRKILKSQLERAV"
     gene            4020..4298
                     /locus_tag="PKL93_08275"
     CDS             4020..4298
                     /locus_tag="PKL93_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71292.1"
                     /translation="MIKGKINVSCQALENEPLQGTGIVTRMAQSAIMGGASAIRANGV
                     EDIREIKAKFEILLVGLIKRQSESFPVYYHYIGRCNRCPSIRSRCGSD"
     gene            4347..4574
                     /locus_tag="PKL93_08280"
     CDS             4347..4574
                     /locus_tag="PKL93_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71293.1"
                     /translation="MRRFFPDRLIMADISNVENVRVVDRLMPDKITTTLSAYTTDTSQ
                     RLKPDIDLVSMLVKEFDYPVMPKGTTWSQTG"
     gene            4704..5120
                     /locus_tag="PKL93_08285"
     CDS             4704..5120
                     /locus_tag="PKL93_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ71294.1"
                     /translation="MILKLAGKDLIVMGAGIAGIRAAIAAPRRGLDVLLVERNGSVGD
                     LSTAGLCSPFIRFWLGNESFVSGIFKEVLYGLHRRGGLLRGSFDLEILKMIYLEKLKK
                     AGVVLSFRSIPVKLISAGGFMKQISLLVPSVNLRSK"
     gene            5327..6145
                     /locus_tag="PKL93_08290"
     CDS             5327..6145
                     /locus_tag="PKL93_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ71295.1"
                     /translation="MIKALVLGGGGAKGFAHVGVIRALEEKGFVPDLIIGVSAGALVG
                     AGYALLSDSKRLWEISLEICRKGKKLLRFANSAARENRGLLFNAATCFHINTFKALPF
                     GLYLNALKKGLKGYRFSDTRIPFKCVSTIMDSGELFVHEEGSIFEALRASMAIPGIIS
                     PFSHKGFSLADGGIVNNIPVSIAKEEGCTLVLAVDLSSNNRKVGIDTSNSILEIIDVY
                     KVEEFQRKELALADVVISPLNKRNIGLLDFSSCIELMNESYEETLKFDLIGASQ"
     gene            6142..6867
                     /locus_tag="PKL93_08295"
     CDS             6142..6867
                     /locus_tag="PKL93_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71296.1"
                     /translation="MKIHFGGYGFQGLASIEYLGDFESESRMVLNGLAGYYVAVERAM
                     GKKEALYRLSKFVDEFFVTLEVMDSGALSSKKIVSGKLERIRHCCIWSKSNHIYGIAA
                     RDSYLDGIAGETELSAEVFNLETRKVELIRDDMREVAAACISIPGLFAPYNDVYVTTT
                     YLSQIPVSFLSEGDIVILNLRNLSTDKPSTASEIIFRAMEIRSLNYAKMILANSSCAS
                     VRVKTRVHSDNVFDYLDKVRRSV"
     gene            6939..7877
                     /locus_tag="PKL93_08300"
     CDS             6939..7877
                     /locus_tag="PKL93_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNQ71297.1"
                     /translation="MLKTMVVIGTLLAMSFYFINGTKKGIDEIVTIEHDGRIRQSIVH
                     LPPMIQDDKLPVVLVFHGLFGNSNYTKNTYGMTEVGDREGFITVYPEGTGTLNSVLLS
                     WNSGFCCGYAMENDVDDVSYIAELLGRLESVYPIDEKRVYLIGLSNGGMLVYRLMSSY
                     PDLFAACAIVSSSPAGGASEDSVVMIEPPDTAIPLIVFHGMKDPIIPFDGGFSSSSEA
                     NGIYFPSIEESVKLWAEKMGAVNMSEKSLENGLVLLREYTGEDERSLVHFYAITDGDH
                     TWPGREKGIDALSSSSQANIKASELIWEFMKDKHLD"
     gene            complement(7902..8174)
                     /locus_tag="PKL93_08305"
     CDS             complement(7902..8174)
                     /locus_tag="PKL93_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71298.1"
                     /translation="MRERSRNLFVVNGLIKDILFEDAFGDVLISGHVFGDFVNEFSEM
                     LRVVENDGTMLLFPTKCNSANNIHRSLTGKGFERIRFEQPGAGFVR"
     gene            complement(8180..8368)
                     /locus_tag="PKL93_08310"
     CDS             complement(8180..8368)
                     /locus_tag="PKL93_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71299.1"
                     /translation="MSLVWIDVDNLNIRPLLLLEKHNVSHLVREFKGVGRLPFAATDR
                     GASLVCAVQPMRSLRRLK"
     gene            8454..9254
                     /locus_tag="PKL93_08315"
     CDS             8454..9254
                     /locus_tag="PKL93_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71300.1"
                     /translation="MRLNKDLFERRLGIFFMVLWILIMFFYLTWDGWLYEWRDCGYSG
                     PSQHFDLDITDMQYSLSPDNEITYLSTNNLHNLIYLCYLVFLIGWLGLLFDLEWAKQG
                     AMATMGISFVAALSTLNLSDHVYLLQVMYDLVHLSGVVMGAYLFSKYHLKTSKSLPVV
                     LGTWAIYLVSHLIFTPWPFWENVGQAYFSVNQINDLPFFLFGMEYIVVVVIILAVNTL
                     IVKLNGRTSNRFLRVLIPLGVYFLLFMIMYSTGLIIVQNVNMNTCALR"
     gene            9843..10430
                     /locus_tag="PKL93_08320"
     CDS             9843..10430
                     /locus_tag="PKL93_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNQ71301.1"
                     /translation="MRKIALGFLLLLILAVPCTMLFSEEIEMPVVQPPMVVTTLGQSP
                     GALMFRLVCVRNQIACVQEDLLTAEQLAEMAAGENAPKTLVITTGTSLKGMGAAGIDM
                     NFEVKRVEALISKAKELGMTIIGAHIEGMARRVDSTDEKSINTVMPKSDLILVIEDSD
                     SDGFFTNFSNETGIPLVKVKESLEIGPALKKLFQE"
     gene            10493..11785
                     /locus_tag="PKL93_08325"
     CDS             10493..11785
                     /locus_tag="PKL93_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HNQ71302.1"
                     /translation="MYFHAGLILFVIAVSFGVAKALKLSVELSMLVAALAAAIVHSGG
                     AIPVRHLVEGSFTYLDVCLIFITATFFMNLIKESGGVAFLVRGIVTAFHKSRVLCLFF
                     LTLLLLIPGALTGSGATTVLTVGTLVGTVLTYMGISEKRVAAIIFVCAAMSAAAPPVN
                     LWAMMAAAGSNMPYVGFFIPLLVLSLAGSLFAMFFLGGKGKKVELDEALKELPEPPAK
                     MNWIRVGMPFVVLIFLIMAGRIWPHSIPTLGLPFMFVVASIFAIILSPKKLEILRIAS
                     DTINTLIPLVGIMIVVGILIQIMALSGARGLISLAVVTLPMWVLFAALWLILPVSEGL
                     LQYAVAPLLGVPLILLFNMKGMDAIIALSAMAVMWPVGDILPPTAVVGRATVMELKFK
                     GHYYRDFLKTSLIPMGFILLIATLFLIFSNELSFLVGG"
     gene            11813..11977
                     /locus_tag="PKL93_08330"
     CDS             11813..11977
                     /locus_tag="PKL93_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71303.1"
                     /translation="MAILYTIISVCYYLITAFIIFVIARSLVKTKNFQEAILFSVMLM
                     PFILRLLRIK"
     gene            11989..13155
                     /locus_tag="PKL93_08335"
     CDS             11989..13155
                     /locus_tag="PKL93_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNQ71304.1"
                     /translation="MTDRNLAIVKIVSCVLVGIALIISGVEFYNHRNFKEPVVVGSGV
                     TEVKTLGDYFAPLKGTINDTNIYIIDSGVPGGTAMLIGRSHPEEPATNLAAKIFVENA
                     KLTKGRLIVVITANQSASRVTRPGDAYPMYYTIETPWGESKFRMGDRWSNPLDSWPDP
                     EAYVHYPSGQLLAYMDIRNFNRTWPGRENGMITEQTNYAFMNLIREENVDIFIDLHEA
                     ELEYPVISTIVAHQKAADIAGLASMILTSTEFRIGMEFSPPSLHGLSHREVGDHSDAI
                     SLLFETPEPFLDRVRGITDEALLLTGKDEFVMTAGKFGLLYEKIDENGWPIDVRVGRH
                     CSSTLMVIELWGDFNPGKEVVIEGVPRYDEVVSNGTGYYFHDPSKARTQDIYYE"
     gene            13200..14765
                     /locus_tag="PKL93_08340"
     CDS             13200..14765
                     /locus_tag="PKL93_08340"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNQ71305.1"
                     /translation="MKKLLLTAILIVSLFYVSAVACTDILAAKQATVDGSVITSHTCD
                     GRYDSRLVVVPAMDHEEGAMAPVYEWIVYADRMDLVKLGEIPQVAHTYQYFNVAYPMA
                     NEHQLIIGETTLGGARETANSDKAIMTIEQLEIFALQRTTNARDAIKLIGALAEEYGY
                     RESCWLGECITISDPNEVWVFEVYGTGPLWEPGMGPGAVWAAQRVPDDHITTVVNYSM
                     IGEIDLETNRPPNVSAGDFMISEDYVQVATDLGLYDPASGEPFVWKYIFGDIEGKKSD
                     RLWRVFSVLNPSGNWSLDNTWEYPFSVKPDEKVSVYDIIELYRDVSEGTPYDMGDNEA
                     WYYESGGEMVKSPLASSEPNTPMRNLLGIPYTRTIAKNGCSYYFVSQARDWMPDKIGG
                     LLWFGLDNPRKGAWIPVYVGTLPNTLPESWVTLNRDELDRSCSYWAFDLVDKISNQRL
                     GALMPLIEEVRVPFQTEMFEEQKDLDEMAMTLYEDNPELVLRLLSSYTARKMREAEEL
                     WYDLSEVLLTKID"
     gene            14824..16419
                     /locus_tag="PKL93_08345"
     CDS             14824..16419
                     /locus_tag="PKL93_08345"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNQ71306.1"
                     /translation="MRRALIVMLVLIVGYGVTAMACTSILVGKDASVDGSTMVTHTCD
                     GSYDARLQVIPGGVHEEGETVSIYKGLCQAGIPGRTVQYVGEIPQVPETYTYFHTGYP
                     FMNEHQVIIGETTWSGERALRNSQGWMTIEQLQVLGLQRGKTAREVIQIMGELAEKYG
                     YGDGGEGLTVIDGDEAWLFEICGPGPFWSPGSEEPGAVWVAQRIPDDHVLVHANRSRI
                     GEIDLENTDYFMASPNVYSTALELELWDGQEPFLFYAAYGPKNAFYNTRREWRVLDLL
                     APSLELDPWAERYPLSVKPDKKVSAQDLMMIKRDHYEGTEFDLTVGLAAGPFGNPNRY
                     ATPASARPEGINYDGWERAISMFRCSYVIVGQARNWMPDAIGGVVWFGEDAPHSTVYI
                     PFYAGATSVPESFSQGARDFFDRSSAWWAFNHVSNLADLRYNVAIEIIKEEYTKFETE
                     FLANQPLIEKVALDLYAENPEAAIDFITNYSNGLANRVVDRWWKLADELIYTLNDGYV
                     DGKTVGYPTWWLEAVGYGDTTAR"
     gene            16772..17395
                     /locus_tag="PKL93_08350"
     CDS             16772..17395
                     /locus_tag="PKL93_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNQ71307.1"
                     /translation="MSLLKIATIFLILIASSVLCSLSIEPLPVIEDPVLFTVWGESIE
                     LQNINYFCDSLQVARDYSPGASIEDLCEGAGFRIEEELSGESFHPFYVSGTPYRTLVI
                     IAGSSLPESRADIERIEELASSVKSSNGLVMVIDIDVEGSGDDPLKQEFAQRLVQYLD
                     VLIVAERPVGEYLSFLEGETPIVVELPVVVDLVSIFERDFGGGRCCD"
BASE COUNT         4777 a         3417 c         4278 g         4973 t
ORIGIN      
        1 gcccagtgtt cggaggacat tcagcttaag aaattgtttg aaatgctcgc aaatgatgaa
       61 cagaggcatt atgagatcat aagtggattc aaagacaaga attacaacta caaaggcaca
      121 tatactttca agaccactag aaatatgttt tccgaaatgc tcaatgataa gaaatgcttt
      181 gagattgatg ctacgaatct tgaagcttat gagcacgcag ttgaaatgga aaaagaaagt
      241 gttaagcttt atcttgatca ggcaaagcaa acaagtgaac catccgaaaa agagatatta
      301 ttgaagctgg ctgcggagga aaacaaacac cagataattt tagagaatct catggatttt
      361 gttagaaagg gcctcgattg gaccgaatcc cctgaattca gccatttgga agaatgggat
      421 aagtttacgg acctagacaa gtactgattt ggttttactt caaatataag gaaagaccgg
      481 gacatcgtcc cggtctcctt aactcagggg taatccatgt cgccttcgtt tgtcttacat
      541 tttaaagggg ttgtgcctgt ggtttctctg gtctctgata atcagacttg atacccgaga
      601 aaaaggttca gtcggtaaga agtttttcag ctgtttgaag atcctcagga gtgttgaccc
      661 ccagaaattc ctctttgttg cttgttgaaa atccgcatac agacttgttg tccgacaatg
      721 ccaaagcaac aatatctgca atgtaaattt ctccagtctc cttgtttggc acaagctgac
      781 cgatgtactc ctgaacaatc tcctttttga tgattatcgg accgatataa aactcccagg
      841 gaggtgggaa gtcagggatc tttctttctt cgaaggagac gactttacca ttttcatttc
      901 tctcgaccag tgcataggga aacttttcct gggtgattcc agagagaaaa gtaatatcac
      961 agtcaccgtt cctgtgaagc tctaccagag atttcaaaga ctcctttgct ataagaacga
     1021 gatcagagta catcactaga atgtctgagt tgtctgggat caggtgaagg ctcctcttca
     1081 aagcgtcagc ggttcctttg ggcgagtcct gataagctaa aagagtattt tcaccgagca
     1141 cttctctgaa ttccttttca aagtcaggat taattacaac aattgagcat ttgctttcac
     1201 aaaaagccga aaccttgttc aagatgtgaa ttatcatagg ttttcccttt atttttacta
     1261 atggcttggg aacctcagac ttcagtctct tgccttttcc agcggcgaga atcacaggaa
     1321 ctaacactat aatccctcct tcctaattat gataacactt ccatcttgtg atgaatattt
     1381 gcgtcctttt tctttgtaag ggttttgcca agtgttatgt ctagaattct ctttgagtcg
     1441 gccttgcatt aattctatga aagagattct atttattcta atgatgacat cactgaaaaa
     1501 agtggcggac ttaggcgacc cattaccaat taggaaaggt tataatctta acttaaggga
     1561 ggttgaggtt tggtggagta tctggatttg tccttaagat tgttttgtgc tctcgttgca
     1621 ggagcgctta taggtgccac aagggaaagg atctataaac ctgccggtct cagaactcac
     1681 tctttgatat gtgttggagc cgcctttata actgtcctct ccacaactgt ctttgtcacc
     1741 gccgagcatg gtgatccggg aagagtagcg gcacagattg tctcgggcat aggattccta
     1801 ggagctggaa caatcctgaa gaaaggtttt tccgtcaaag gcttgaccac ggccgccaca
     1861 ctctgggtaa cggctgctgt cgggatgggt ttcggcagcg gggaatactt gctctcggta
     1921 gtagtgacgg tcttcgcgtt gcttatagtt cttttcctta aaaggattga gttgtttatc
     1981 ggagggagaa atcttcccaa agttttgata gttgctgaaa acagtcaaga aacgtctaga
     2041 aaggtatccg aatggttcaa ggacaatgga ttgacggttg agtcaatgga aattgaagag
     2101 gacgatgaag tccttagtct cttagttgag gtgagcaaag gagatgccat aaaggtaaaa
     2161 aacattatgg tgggtctttc cacactgaag ggtgtcaaga gcgcagagct tcgatgactt
     2221 ttattttctt ggacagatat ggtctatcca gagttcctga cctcctatcg aaaaagacgt
     2281 tatccttccc tggagattga gtactccttt aggaccgctc acgcttgtaa gggttacggt
     2341 gaccccgccg attaccttac cgtcgatgtc tgagaagtcc tggacgtttt cgagaatgcc
     2401 gtttatctcg acattgatgt tgcctccata ttcaccgtag tagagaatca gagcggatgg
     2461 ttttaccggg aacttgaagg aaacggtgat gttgttcagg ttgacgtcct tccctgaatg
     2521 acctgccaac ccgccgttgg taacagtcat cataccattg gaagtcggtt gccatttgac
     2581 catacgaatg gcttcatatc cattcgagtt gtctgctcaa acagggtatc gccattgtag
     2641 tactttgtgc caacggttga tccctcgaaa tcaacacagt ttagcaccaa gtagcatccg
     2701 gaaatgacaa agatacacac agcaactaac aagaaaacaa ctatccgttt cattaaatcg
     2761 cctcccctga gatcttgcaa agataaagta agaatagaat aaggtatgat ttggacgtga
     2821 caattatatt cttacgacga tcgtggtcaa agaagacgaa tcgctatatt tccgggattt
     2881 gcgtttcagt tgttgaaacc acctgcaatc agtacaagat tccgaaagga gaaagacctt
     2941 atttcttttc atggggcagt tatctggtct atttgaatgg gcgatacgat ttgatgatgt
     3001 agaacctatt tagcggtttt tccataatct aatcacaagt catttatatc actttgtttc
     3061 gaattgccat ctaaaaatga tttatccctt ggagcgctct aatagaaact aattctctgg
     3121 gaaggtttgt gaaagtttag ggtgcagttg tctgagctat ccgcaatacc gtaggtgatt
     3181 ttttgtgttt cttcattatg gtaaaactct atttcttaag aacttgcttc ctgcaaaaag
     3241 tgtaaatagt tttttggggg aaatggtgtg taatgttttc taaaagagtc gtttttctac
     3301 cagtagatga acgtttctgc acaagggatt actttcttct cctttgtgag gccgccggag
     3361 gagtgttaga gaaaggaagc gatgagatac cgctaattgt gaataacccc accgacaagt
     3421 tgaatgcaga ggatcaaccg gagaatcaag ggaagtcaac ttccttcgaa agagttttca
     3481 ctctcctcaa gaggtttgtg agtgaagtta cagtcattgc agatgtcaag tattcaaatg
     3541 gttccgaaaa caagatcata gaagcaatta tggaaattca taattttgac tggaattcaa
     3601 cgaattattc gggatggaat acggccggta acaccattgg tactgtcagt gcacattcag
     3661 tagttcaact tctagtaatg aaaaatttct tgtccctaga caaagtgaag atgaagcacc
     3721 ttcagataat tatcttcctt gaacattggg catataagtc gaaaataagg aagattctca
     3781 agtcccagtt ggaaagagcg gtttgaatcc agtgaatctt attcctttag agaaatgggt
     3841 agaggatttt gtgattgaga aaatgggaag ctactgcgaa agagtgaaga gttctatgga
     3901 tcttgatttc gaaaaataga aagagtcttc tttcccaggc acagaatctt cgaaatcggt
     3961 atttcattaa gagaaagaaa ggatctaatg tagtgccgct aaaaagagag ttcttaagta
     4021 tgattaaagg aaaaatcaac gtctcctgtc aggcgctcga gaatgaacca ctacagggaa
     4081 ctggaatcgt gaccagaatg gcacagtcgg caataatggg cggagcttct gcgattagag
     4141 caaatggagt tgaggacata agagagataa aggccaaatt tgagatcctt ttagttggtc
     4201 tgatcaagag gcaaagtgaa agtttccccg tgtattacca ttacatcgga agatgcaatc
     4261 gatgtccttc aatccggagc agatgcggta gcgattgact gtactctaag agataggcca
     4321 gagcctattc acatgatttt cgaaaagtga gaagattctt tccggataga ctgataatgg
     4381 cagacatttc taatgtggaa aatgtacgtg tggtcgatag gctcatgccg gacaagatta
     4441 cgacaaccct ttcagcgtac accacagata cttcacaaag actgaagcca gatattgacc
     4501 ttgtctctat gcttgtgaaa gaattcgact acccggtgat gccgaaggga actacttgga
     4561 gccaaaccgg atgatcatgg cgtttgaggc agaagctttt tcagtttctc tagggagtgt
     4621 aatcactaga cctaagctca tcaccaagag attcaatgat tgcctggaaa catttaagaa
     4681 gaacagtgat ttcagagaga gtgatgattt tgaaacttgc aggtaaagac ctgattgtca
     4741 tgggtgcagg aattgcggga atccgtgccg ccattgccgc accaagaaga ggccttgatg
     4801 ttctgcttgt agaaaggaac ggttctgttg gagatctttc aaccgccggt ctttgcagtc
     4861 ccttcataag attctggctt gggaatgagt cgtttgtatc ggggattttc aaagaagtgc
     4921 tttacggtct tcacaggcgt ggtggacttc tgaggggttc attcgatttg gagattttga
     4981 agatgatcta tctggagaaa ctcaaaaagg ctggagtcgt gctctcgttt cgctcgattc
     5041 cggtaaaatt aatctccgca ggagggttta tgaaacagat ttcgctgctt gtgccaagtg
     5101 tcaatttaag aagtaagtag atgaaagctc taggagtgga catcggtggt actatataaa
     5161 gactgcggtt gtggcatgct cgggtaaaat cagtcaaagg aagaatcatg caactagtgg
     5221 gaatcttaag aatcagatcc tcacgattat tactgaaggg atttcttcag attgcgtttc
     5281 atctataaga atagggaccg cagggacatt atttcgaggt gtttagatga ttaaggcgct
     5341 agttcttggt ggaggtgggg caaaaggttt tgctcacgtc ggagttattc gagcgctaga
     5401 agaaaagggt tttgtgccgg atctgatcat aggggtaagt gccggcgcac ttgtaggggc
     5461 cgggtatgct ttactctcag attcgaagag gctttgggag atctctcttg aaatctgtag
     5521 gaagggtaaa aagctgcttc gctttgcaaa ttcagctgca agagaaaata gaggcttgtt
     5581 attcaatgca gccacttgtt ttcacataaa cacttttaaa gcgctgcctt ttggattgta
     5641 cttaaacgcg ctcaagaagg gtctgaaagg gtacagattc tctgacacaa gaattccctt
     5701 caagtgcgtt tcaaccataa tggattcagg cgagttattc gttcatgaag aaggaagtat
     5761 tttcgaagcg cttagagctt ccatggcaat tcctggaatt atctcgcctt ttagtcacaa
     5821 aggcttctct cttgctgatg gagggattgt gaataatatt cctgtcagca tagctaagga
     5881 agagggctgt actctagtgt tggccgtgga tctttcttca aataatagaa aggttgggat
     5941 cgatacttca aattcgatac tggaaataat cgatgtgtac aaagtcgaag agtttcaaag
     6001 gaaggaactg gctcttgccg atgttgtcat ttcaccattg aacaaaagga atattggttt
     6061 gcttgacttc tcatcatgta ttgaactcat gaatgaatct tatgaagaaa cattgaagtt
     6121 cgatctaatt ggagcaagcc agtgaagata cattttggtg gttatggttt tcagggactt
     6181 gcctctatcg agtaccttgg agatttcgag agcgaaagca gaatggtttt gaacggactg
     6241 gcgggctatt atgtagcggt tgagagagca atgggcaaga aggaagcctt gtataggctt
     6301 tcaaagtttg tggatgagtt tttcgtcacg ttggaagtga tggatagtgg cgcactttct
     6361 tcaaaaaaga tagtgagcgg caaattagaa cgaattcgcc actgctgcat ttggtccaag
     6421 agtaaccata tctacgggat agccgcaagg gattcttatc tcgatggaat tgcgggggag
     6481 acagagctta gtgctgaagt gttcaatctt gaaactagga aggttgagct catccgcgat
     6541 gatatgagag aagttgcagc cgcctgtatt tccatcccgg gacttttcgc tccttacaat
     6601 gatgtttatg ttactacgac ttatctgagc caaattcctg tttcgttcct ttcagaaggt
     6661 gatattgtaa ttctgaatct aaggaatctt tctactgaca aaccaagtac agcttcagaa
     6721 ataattttcc gcgccatgga gatccgatca ctaaattatg caaaaatgat tctcgctaat
     6781 tccagttgcg cttcagttag agttaagacc agggttcatt cagacaacgt atttgactat
     6841 ctagataaag ttcgaaggtc agtttagttt gagcgaatga tgataatata gattgtgtgg
     6901 aatgctgaga caagcggctt cgaatggagg aattctaatt gctcaagacg atggtggtga
     6961 tcgggactct tctggctatg agtttctatt tcatcaatgg aacaaagaaa ggaatagatg
     7021 agatcgtgac gattgaacac gatggcagaa taagacagag tattgttcac ctgcctccga
     7081 tgattcaaga tgataagcta cctgtagtgc tggtatttca cggtcttttt ggcaacagta
     7141 actacacaaa gaatacgtac ggaatgactg aagtgggaga cagagaaggg tttattaccg
     7201 tgtatcccga gggaaccgga acattgaaca gcgttctttt gagctggaat tcaggtttct
     7261 gttgtggtta cgcaatggaa aatgatgtcg atgatgtctc atacatagcg gagctccttg
     7321 gaagattaga aagtgtttat cccattgacg aaaagagagt ctatctcatt ggactttcca
     7381 acggagggat gctcgtatac aggctcatgt caagttaccc cgatctcttc gctgcctgtg
     7441 cgattgtttc atcttctcca gccggcggag ccagtgagga cagcgttgta atgatagaac
     7501 ctccagatac cgccattccg ctcatagtgt ttcacggtat gaaagatcct attattcctt
     7561 ttgatggcgg tttttccagt agttctgaag caaatggtat ctattttccc tcgatagagg
     7621 agtctgtgaa actttgggcg gagaaaatgg gcgcagtcaa tatgagcgag aaatcactcg
     7681 agaacggttt ggttttactc agagaataca ctggagaaga cgagagaagt ctagttcatt
     7741 tctacgcgat aaccgatgga gatcatacct ggcctggaag agagaaggga atagatgctc
     7801 tcagcagctc ttcacaggcg aacatcaagg caagtgagtt gatatgggag tttatgaaag
     7861 acaagcatct agattgatgc tttgttcgct ttaggtagat ctcacctaac aaagccggct
     7921 cccggttgtt caaaccttat gcgttcaaat ccctttcccg taagagacct gtgtatattg
     7981 ttagcagagt tacatttcgt tggaaagaga agcatagtgc cgtcgttctc gacaactctg
     8041 agcatttcgc taaactcgtt aacgaaatcc ccaaagacat gaccggaaat gagaacatca
     8101 ccgaatgcgt cttcgaagag aatgtccttg attaggccat tgacaacgaa gagattccga
     8161 cttctttctc gcatgctttt tattttaggc gtcttaaact cctcattggc tgaactgcac
     8221 acaccaatga ggctcctcta tcagtagcgg caaatggcag tctaccgacg cctttgaact
     8281 ctcttacaag atgactaacg ttgtgctttt caagaagaag caggggtcta atattcaaat
     8341 tgtccacatc tatccagacg agagacaaaa catcaccccc ggagcgaaag ctaataagga
     8401 tagtagcatg ataaaatcgg gattatcttc agttacgtag atgggagact tatatgaggc
     8461 ttaacaagga tttattcgag agaaggctgg gaatcttctt catggtattg tggatcttaa
     8521 tcatgttctt ctacctcacc tgggatggat ggctgtacga gtggcgcgac tgtggctaca
     8581 gcggtcctag tcagcacttc gatcttgaca ttaccgacat gcagtattcg ctctccccag
     8641 ataatgagat cacttacttg tctacgaaca atcttcacaa cttgatatat ctttgttatc
     8701 tcgtcttctt gatcggttgg ctgggtttgc tttttgacct tgagtgggct aaacagggag
     8761 cgatggctac catgggaatt tcttttgttg cggcgctctc gacgctaaac ctctccgacc
     8821 acgtgtactt gcttcaagtg atgtatgatt tagttcatct ttcaggggtg gtgatgggag
     8881 cgtatctgtt ttcgaaatac catctgaaaa cttcaaaaag cctccccgtt gtcctaggca
     8941 cgtgggctat atatcttgtt agtcacctta tcttcactcc ctggccgttc tgggaaaacg
     9001 ttggccaagc ttatttcagc gtaaatcaga tcaacgactt gccgttcttt ctttttggta
     9061 tggagtatat agttgttgta gtaatcatac tcgcagtgaa cacccttatc gtaaaactaa
     9121 acggaagaac gtcgaaccgt tttctaagag tattgattcc acttggcgtc tattttcttc
     9181 tcttcatgat aatgtactca acaggcctaa taatagtcca gaacgtgaat atgaacacat
     9241 gtgcccttag ataatcatgt aagcttgagt tgtccaagtt cgttcttctt tttcaaaatc
     9301 tgacctcaac atatttcttc ttagagtcgt agtatctttt ggtgtttctg aggtccttct
     9361 acccgtttgg cttcagggac atatattcat aaccttctta ggtcttccat ctttttatca
     9421 aatgcctgcc gagagagatt tgcagtctcc atcgtaacac gcatagacaa agaatgtgga
     9481 gaagaagact tgatgaaagc caatgaagtt aactgaatcg aaaggaaatg aaagcaaaga
     9541 attaattgat gaataggggc actcaatcaa tcgcatctca atcaactatg agttgataat
     9601 tgccaatagt tggatcctat cgggtaccat gtcggcagac aatagtttgt cagaaacata
     9661 gtagatggta gaatgaattt gtaaacaata aggattacgt acttatttca caatttatat
     9721 cttattcctt cggcagtctg caagagaagc atttgaacta agttatcatc agcattattt
     9781 actgtagaca attgaaaatg ggtctataag cctgtttatt ggttaataaa ggagggtgtt
     9841 ttatgagaaa gatagctttg ggatttcttt tactgctcat tctagctgtt ccttgcacca
     9901 tgctcttctc ggaagagatt gagatgcctg ttgtccagcc tccaatggtt gtcactactc
     9961 tgggtcagag cccgggtgct ttgatgttca gactggtctg tgtcagaaat caaattgcct
    10021 gtgttcagga agatctcttg actgccgaac agcttgccga gatggcggca ggagaaaatg
    10081 ctccaaagac attggtcata accacaggaa cgagtctcaa aggaatggga gctgccggta
    10141 ttgatatgaa tttcgaggtg aaaagggttg aagctcttat ttccaaggct aaagaactcg
    10201 gaatgacgat tatcggtgca cacattgagg gaatggcgcg ccgtgtagat tctactgacg
    10261 agaagtcaat aaacacagtg atgcctaaat ctgatttgat tctcgtaata gaagacagcg
    10321 attcagatgg attcttcaca aacttttcga atgagactgg aattcctctt gtaaaggtga
    10381 aagaatcctt ggaaatcggg ccggctctca aaaaactatt ccaagagtag agcataacaa
    10441 tgatcttctt atctgtcgac ctttaggtcg attctaaagg gggagtgtcc tggtgtactt
    10501 ccatgctggt ctgatacttt tcgttatagc agtatcgttt ggagttgcaa aggcacttaa
    10561 gctaagtgtc gaactgtcga tgcttgttgc cgcgcttgca gccgctattg tccactcggg
    10621 tggagcgatt ccggtacgtc atcttgtgga aggctccttc acttatcttg atgtctgtct
    10681 gatctttatt accgcaacgt ttttcatgaa cctgattaaa gagtccggtg gagtcgcttt
    10741 tcttgtaaga ggtattgtca cggcctttca caagagcaga gtactgtgtc ttttctttct
    10801 tactttgctg ttgttgattc ctggggccct tactggatcg ggtgccacca cagttctcac
    10861 ggttggtaca ctggtgggaa cggtcctcac ctatatggga atatctgaga agcgggtggc
    10921 ggcgataatc tttgtttgcg ctgcgatgag tgccgctgca ccgccagtaa acctctgggc
    10981 aatgatggct gcggccggat ctaacatgcc ttacgtgggt ttcttcatcc cactgctggt
    11041 attgtcgcta gccggttctc tatttgcaat gttcttcctt gggggaaagg gtaagaaagt
    11101 agagcttgat gaagctctga aagagcttcc tgaaccacct gctaagatga actggataag
    11161 ggtaggaatg ccatttgttg ttctcatttt cctgataatg gccggaagga tttggcccca
    11221 ctcaatcccc actctgggac ttccgttcat gttcgttgtc gcatcaatat ttgcaattat
    11281 acttagccca aagaagctag agatccttag aatcgcatcg gataccatca atacattgat
    11341 ccctcttgta ggcatcatga tcgttgtagg tatcctgatt cagattatgg ctttgagcgg
    11401 cgcaagggga ttgatctctc ttgcggtagt aacgttgccc atgtgggttt tatttgcagc
    11461 gctgtggctt attcttccag tgtcagaggg attactccaa tacgcggttg caccgcttct
    11521 aggagtgccc ttaattcttc ttttcaacat gaagggaatg gatgccataa ttgcactctc
    11581 tgcaatggct gttatgtggc ctgtgggaga tatccttcct cccacggcgg ttgttggaag
    11641 agcgacagtg atggaactga agttcaaagg ccattattac agggatttct tgaagacttc
    11701 gctcataccg atggggttca ttcttctaat tgctactttg ttcctgattt tcagtaatga
    11761 actctccttt ttagttggcg ggtgatgtca tgagtgaaag gaagtgtttt tgatggctat
    11821 tttgtacacg ataatcagtg tctgctacta cttgataacg gcttttatca tctttgttat
    11881 agctagaagc ttggtgaaga cgaagaattt ccaagaagca atcctgtttt ctgtcatgct
    11941 catgccgttc atcctgcgac ttttgagaat caagtagggg gtgctgcgat gactgacaga
    12001 aatctggcga tagtcaaaat agtatcatgt gttttagttg gaattgccct aattatctcc
    12061 ggtgtagagt tctacaatca caggaatttc aaggaacccg tcgttgttgg atcgggagtc
    12121 actgaagtca aaacgttagg tgactacttc gctccgttga aaggaacgat aaacgatacg
    12181 aacatctaca taattgacag cggtgtaccg ggtgggacgg ctatgttgat aggcaggtcg
    12241 catcccgaag aacccgcaac gaatctggct gccaagatct tcgtcgaaaa cgcgaaactt
    12301 accaagggga gactcatagt tgttattaca gctaatcaga gcgcttcaag ggttaccaga
    12361 cctggggatg catatcctat gtactacaca atagagactc catggggaga gagcaagttc
    12421 aggatgggag atcgatggtc caacccgctt gactcatggc cggatcccga agcgtatgtt
    12481 cactatccta gtggtcaact cctggcttat atggatattc gtaatttcaa cagaacgtgg
    12541 cccggaagag aaaatgggat gatcaccgaa cagacaaact acgcctttat gaacttgata
    12601 agagaagaga atgtagacat atttatcgat ttacacgaag ccgagctgga gtaccctgtt
    12661 ataagcacta tagttgccca tcaaaaggcg gccgatattg ccggactggc ttcaatgata
    12721 cttacctcaa ctgaattcag aattggtatg gagttctcac ctcctagcct gcacggactt
    12781 tctcacagag aggtcggtga ccattccgac gccatatctc tgttgtttga aacgccggaa
    12841 ccttttcttg acagagtcag gggaatcacc gatgaggctc ttctcttaac gggtaaggat
    12901 gaattcgtta tgacggcagg taagttcggt ttgctctacg agaaaattga cgaaaatggt
    12961 tggccgatcg acgtaagggt tggcagacac tgttcttcaa ctcttatggt tattgaacta
    13021 tggggtgatt tcaatcctgg aaaggaggtt gtgatcgaag gagttccaag gtatgacgag
    13081 gtagtaagca atggaacagg ctattatttc catgatcctt caaaagctag aacacaggac
    13141 atttattacg agtaaccaag aacatttgtg attgataatc acaaaggagg ggagtgaggg
    13201 tgaagaaact gttattgact gcaatactga tagtctcact gttctatgtg agtgctgtag
    13261 catgtacgga tatcttagca gcgaagcaag ccactgtaga tggttccgtt atcacatctc
    13321 atacatgtga tggtcgttat gactccagac tggttgtcgt accggctatg gatcacgaag
    13381 agggagctat ggccccagtt tatgagtgga tagtctacgc cgatcgaatg gatctggtga
    13441 agcttggaga gattccacag gtagcacata cctaccagta cttcaacgtt gcttatccta
    13501 tggcaaatga acaccagctg ataataggag agacgacact tggtggagcc agagagactg
    13561 ccaacagcga taaggcgata atgacaatcg aacagctgga gatcttcgct ctacagagaa
    13621 caactaacgc cagagacgct ataaaactta taggcgctct tgcagaagag tatggttacc
    13681 gagaatcgtg ctggttaggc gaatgcatta caatttccga tccgaacgaa gtttgggtct
    13741 tcgaagttta cgggaccggt cccctatggg aaccaggtat gggacctggc gctgtttggg
    13801 ctgcccagcg cgtgcccgac gatcatatta ctaccgtcgt gaattacagc atgattggtg
    13861 agatcgatct tgagacaaac aggcccccga acgtatctgc aggggacttc atgatcagtg
    13921 aagactatgt tcaggtggca acggatcttg gcctgtatga tccggccagt ggagaaccct
    13981 ttgtctggaa gtacatattt ggagacattg aggggaagaa gagcgacaga ctctggagag
    14041 tcttctctgt tctcaatcct tcggggaact ggtcgttgga caacacatgg gaatatccat
    14101 tctcagttaa gccggatgag aaggtatccg tgtacgacat aattgagctt tatagggacg
    14161 tttccgaagg tacaccttac gacatgggcg ataacgaggc atggtactat gaaagtggag
    14221 gcgagatggt taagagtccg ctggcaagtt ctgagcccaa cactcctatg agaaatcttc
    14281 ttggcattcc ttacacaaga acaatagcaa agaacggttg ttcgtactat tttgtttcgc
    14341 aggcaaggga ctggatgccc gacaagatag gcggacttct ctggttcgga cttgacaacc
    14401 cgagaaaggg agcctggatt cccgtttatg tagggactct tcccaatacg ctcccggagt
    14461 cttgggtgac cttaaacagg gatgagcttg acaggagctg ctcttattgg gcctttgatc
    14521 ttgtcgataa gatctctaat cagaggcttg gggctcttat gcctttgata gaggaagtaa
    14581 gagtaccttt ccaaacggag atgttcgaag aacagaagga tcttgatgaa atggcaatga
    14641 ccctttacga agataatccc gagcttgttc ttagactcct ctcatcctac acggcgagaa
    14701 agatgagaga agcagaagaa ctctggtatg atctttcaga ggtcttgtta actaagatag
    14761 actaagtaat tgactttggg ccggttgatg ccggtccatt actttgaaag gggagtgaaa
    14821 gaaatgagac gtgcacttat tgttatgcta gtcctcattg tcggttatgg agtaacagca
    14881 atggcatgta cttcgatact tgtcggaaaa gacgcatctg tcgatggttc tactatggtg
    14941 acacatactt gcgacggcag ttacgatgcc aggttacagg tgattcccgg tggagtacat
    15001 gaagaaggcg agacggtaag tatctacaag ggtttgtgcc aggcgggtat tccaggtaga
    15061 acggttcagt atgttggaga gattccacag gttcccgaaa cttacaccta cttccacacc
    15121 ggctacccgt ttatgaacga acatcaggta attatcggag aaacaacctg gagtggagaa
    15181 agagcgctca gaaactccca gggttggatg acaatcgagc agctccaggt gctgggtttg
    15241 cagagaggaa agacagccag agaagtcatt cagataatgg gtgaacttgc tgagaaatat
    15301 ggttatggtg acggcggcga ggggcttaca gtaattgatg gagacgaagc ctggttgttt
    15361 gagatctgtg gccccggtcc tttctggtct cctggaagcg aagaacccgg agcagtatgg
    15421 gttgcgcaga gaattcccga tgaccacgtc cttgtccatg caaatagatc tagaattggc
    15481 gaaatcgatc ttgagaacac agattatttc atggcatcac ccaatgtata ctcaacggca
    15541 ctggaactcg agctgtggga cggacaggaa ccattccttt tctatgcagc ttacgggcca
    15601 aagaatgcct tttataacac gagacgtgaa tggagggttc tggaccttct tgccccgtct
    15661 ctagagctcg atccatgggc agaaagatat ccgttgtctg tcaagccaga taagaaagtg
    15721 tctgcgcagg atcttatgat gattaagcgt gaccattatg aaggaaccga attcgatctc
    15781 actgttggcc ttgccgcagg tccttttggt aatcccaata ggtatgctac ccctgccagt
    15841 gctagaccgg aaggcataaa ctatgacggt tgggagagag ctatatctat gttcagatgc
    15901 tcatatgtca tagttgggca ggcaagaaac tggatgccag atgcgatcgg tggagttgtg
    15961 tggttcggag aggatgcacc tcactcgaca gtttatattc cattctacgc aggtgcgacc
    16021 agtgttcccg agtcgttcag tcaaggagca agggatttct tcgacagaag ctctgcttgg
    16081 tgggcattca accacgtgtc aaacctagcc gatctgagat ataatgtcgc aatagagata
    16141 atcaaagagg aatacacgaa gtttgagact gagttcctcg caaatcagcc gctgatcgaa
    16201 aaggttgcgc tggatctcta tgcagagaat cccgaagcgg ctatcgattt cattactaat
    16261 tacagcaacg ggctagcaaa cagagttgtc gatagatggt ggaagctggc cgatgagcta
    16321 atttacaccc tgaacgatgg gtatgttgat ggtaagactg tgggttatcc gacgtggtgg
    16381 ctcgaagctg ttggttatgg agatacaact gcaagataga tactgatcgt ttatttgtaa
    16441 gggcggatat tattccgccc ttttttaaag ctatagatgg agtgatccgc gaaaaacatc
    16501 cagtgagtgc aagtgtgctc aaatcagcta attctcaatg cgttgcatat tcgaaagata
    16561 aaacctactc ttcctggatt gattttcgaa tccctgaaag ggtttgagtc tttccgaagc
    16621 taactggagc tttttcctcc tatttaacac ttgtgtatcg attgaaggaa ggaggcatat
    16681 tcttgggacc ctttgctata atgaatatag aaagttctcg aaccttactt atgttgattt
    16741 ttttagtatc tacagagttt attgcttggg gatgtcactt ttgaaaattg ctacgatctt
    16801 tttgattctt attgcgagtt cagttctttg ctcgctgtct atcgaaccgc tccccgtaat
    16861 tgaagatcca gtgcttttta cagtttgggg tgagagcata gagcttcaaa acataaacta
    16921 tttctgtgat agtcttcaag ttgcacgtga ttattcaccg ggtgcaagta tcgaagatct
    16981 gtgtgaaggc gccggattca ggattgaaga agagctttca ggagaatctt tccatccatt
    17041 ttatgtttca gggacacctt accgcacact tgtgataatt gccgggagtt ctttgccaga
    17101 gtcgagagca gacatagaga gaattgaaga gcttgcttcc tcagtgaagt cttcaaatgg
    17161 attagtgatg gtaatcgata tagatgttga aggatcggga gatgatccac tgaagcagga
    17221 atttgctcaa agattggtgc agtatctaga tgttctgatt gtagctgaaa ggccagttgg
    17281 agagtatctg tctttcttgg aaggagagac tcctatagtt gttgagttac ccgtggttgt
    17341 agacctcgtt tcgattttcg aaagggactt tgggggcggc agatgctgcg attgattgga
    17401 ggaaatatga agagagttct cttgattacg atgattttga ttggc
//
LOCUS       DAOQYI010000033        16589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_3904_length_16589_cov_73.269626, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000033 DAOQYI010000000
VERSION     DAOQYI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3904_length_16589_cov_73.269626"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            641..1678
                     /locus_tag="PKL93_08355"
     CDS             641..1678
                     /locus_tag="PKL93_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71308.1"
                     /translation="MNKKYPSITKLILAGVLGLLALLIPFTAGKILLAIAGIVVVALS
                     LKKFKWLLITLGILFLVIPIVALGVINGIGSNCLNWPLGFIDDPSILQWFNWIENDGY
                     EYYGNNEGGYNRKGSDHKTYMPDQYVDGAKEIVFSGLGFEISFDENSDKIHIPSKLEI
                     NRYGERLQISMPNDMKNSTAQITIGTLSELDRLEFRTVFLRLNDKVNVAKMEIQTISG
                     EIRGEIMASKMISIEGTSLSITGSLKAPEIIIENVPSLSFMGSVEAEKFTVQNGVALA
                     FSMDASKIENLSIHGTVVNGRIKFSDSWTGSRRVNVSGIGGSLNLLMPTGSGGFELIT
                     SGRVSVVTEKY"
     gene            1692..2048
                     /locus_tag="PKL93_08360"
     CDS             1692..2048
                     /locus_tag="PKL93_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ71309.1"
                     /translation="MFGKIDKHDGVPAYIQIMNIVKREIMLGRLENGDQLPPVREMAA
                     MFGVNMNTVIRSLERLQFEGLVEAEHGVGYFVKTAHSINREVMKTISETVGELKKNGI
                     DLQMAKVLLEEVWKND"
     gene            2041..2622
                     /locus_tag="PKL93_08365"
     CDS             2041..2622
                     /locus_tag="PKL93_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71310.1"
                     /translation="MTEKIEAYFMKEFSEKAGRIAVFSLGLLIPFYPVRMIFLFLLAT
                     SLLPSDIQHKRLGSLLALPYSRQELFWISYLFLIALSVVTQVIGMALFGASLISSMNI
                     YWLSMVIGSISFSTAYFAVSMISVTAGLDNFGIPFLVLIADLIAGGVGNRFSNPYFYA
                     SPAHQGNVWISGAVSLAVLLVANYLFVKKGVQK"
     gene            2619..3461
                     /locus_tag="PKL93_08370"
     CDS             2619..3461
                     /locus_tag="PKL93_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71311.1"
                     /translation="MIVSVDKLRKSFSSKPVLNDISFNVEQGDIFAIVGPNGAGKTTT
                     LRCIFGEIAPEGGEVKVFGENVTSRTKEKIAVMTEDRLTFRRFLGEDYLRMWRMLYPG
                     WQEKVFASFAVHYKFNLNERVETYSMGMKTLFHIALTVASGADLLILDEPTQSLDPVI
                     RQEVMGVLRDYVLQEGKTMIISSHEIYDLEEIAGSFAVVLEGKVLYSDTIDGAKETHR
                     IIPKGQAIPSGEIIGVTGEETLVRTDEEAGRFPNFKEIVLGYLQSKKAFAPFKEEFRP
                     RFKI"
     gene            3846..4343
                     /locus_tag="PKL93_08375"
     CDS             3846..4343
                     /locus_tag="PKL93_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71312.1"
                     /translation="MEEENVKKETVKKGSGLSQIFLGLIVIALGLVIIFVPAVLSNFW
                     LLFIWLPGVIMEYNAFRNRNNGLLVPGGILLVVALALTLGVIFPGFYSQGGWGLFVLA
                     PAFGLLQLYFADGRRNFGLLVPIAILTAVGVIALIPSSLSQWTMIVVGAVLIVSGLLV
                     LFRRK"
     gene            complement(4344..4901)
                     /locus_tag="PKL93_08380"
     CDS             complement(4344..4901)
                     /locus_tag="PKL93_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71313.1"
                     /translation="MENYHSESPLREITLKNGDSCHFYRADIGDSDRIIEYINSVAVE
                     SDFLSFGRGEFTMSSEDERRLISSFKERSNCLMAVAKCDQSIVGLITMQGGSRKRIYH
                     WAEIALSVSMKYWGMGIGRVLIELAVEHAGRNGITRIGLKVRTDNDRAIALYRKMGFE
                     IEGKLKKFMRVNDDYFDFYQMGRDV"
     gene            complement(4882..5211)
                     /locus_tag="PKL93_08385"
     CDS             complement(4882..5211)
                     /locus_tag="PKL93_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71314.1"
                     /translation="MDEKTLESILKLKEDQINAMDFKKLLGYIRSIDEYFRGNSQIDV
                     EIALKAYQKAIQLLTAARLRLITLKKEKEELDQQYSTFLNSIESEEISEEPQERSTEG
                     EDGKLPF"
     gene            complement(5213..6754)
                     /locus_tag="PKL93_08390"
     CDS             complement(5213..6754)
                     /locus_tag="PKL93_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNQ71315.1"
                     /translation="MEEETLEFKDLDNMLEHIRTILADAGLNNLRVKFPADVTNINVR
                     GSYVYINVSQDYVERGTKRRIDLSMISGTSAFARMGRQLALKSSSDLKGKKWLFEGTL
                     SFYKPRASFSIWIDTIAPIGESDIAARRREIYLALKARNALRTEEHDLAELPPIKRIA
                     VISSSTAAGLGDFFSNLSVQYPYKPIVHLFESYMQGNRTVPGILSALKKIASCPVKYD
                     VVVLTRGGGSASDLMYFDNLDLGSAISDFNKTYCPVLSAIGHERDFTIPDFVAWKRFD
                     TPTAVARGITEQVNEYIASLEEFADLLDDEMNWLFQRAWDETDPKRLGIMSDRIEENM
                     SRTEHFFEDEIRVLSGTILHRIGQAVNELGSFNPTRYVLSIERTMNEILALTEGLINR
                     AETTIQRSFHYSESQLDYAGLRNQLDRHLMNANGQVELLDKGIFRLIDDKINEAGNDL
                     DNLFNDLTLHGGYAASLLFGGVVLKKADRIVNSVRIVSPGDEVDCVFKDGEAKATIDE
                     VKSEV"
     gene            complement(6837..7598)
                     /locus_tag="PKL93_08395"
     CDS             complement(6837..7598)
                     /locus_tag="PKL93_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ71316.1"
                     /translation="MKRFEGKVVMISGGSSGIGAETAIAFAEEGAKVVITDVDEEKGR
                     KLAEEINESGGTAVFMKHNVADAKQTKEIINKIVEKFGKLDVAFNNAGIAGPSLPISE
                     YPEEDWERVISINLLGVYYGMKYQIQQMLKQGGGAIVNNSSILGKVGFNNASAYVAAK
                     HAVVGLTKAAALEYASKNIRINAVNPAFIKTPLIENAGMKEGTEMYDMLVGLHPIGRL
                     GNPREVANAVLFLSGEDASFVHGESLMVDGGYTAK"
     gene            complement(7884..8267)
                     /locus_tag="PKL93_08400"
     CDS             complement(7884..8267)
                     /locus_tag="PKL93_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71317.1"
                     /translation="MRRNIIRVALVVVLVALGFGLYVLGKEHTIFVENKDIVVDGVEY
                     KSANTINVIVDDIELEDVKKGKRKVAYSAGPWHRIKAVEVFPDGTTREIERKFTLSPN
                     DTAVINLVALFEVEEGWLTIEQAER"
     gene            complement(8272..9411)
                     /locus_tag="PKL93_08405"
     CDS             complement(8272..9411)
                     /locus_tag="PKL93_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71318.1"
                     /translation="MKDQSLTRAKNPLVRFVISNAVPILFVILLIFAVPLSGFSTDYL
                     LNEIITRLGRNSFLVISLLIPVMAGMGLNFGIVLGAMAGQIGLIFMLDWGVVGIPGIV
                     LAMLIGTPIAILLGMLAGMILNRAKGREMVTSMILGFFINGIYQLTILYGMGSVIPIA
                     SDKLLLPRGYGVRNAIDLISIRKALDNMWSIEISGVSIPLGVFAIIAALCIFVFWFTR
                     TKLGQDMRAVGQDMQVAATAGINVDRTRTLSIIISTVLACYGQIIFLQNIGTINTYNS
                     HDQVGTFAIAALLIGGASVAKATIPNVFIGITLFHLTFIVAPRAGKELLGQAQIGEFF
                     RVFVSYGIIAISLALYAWRRQVEKETERRQAKAAIKAAVEDGTGG"
     gene            complement(9408..10451)
                     /locus_tag="PKL93_08410"
     CDS             complement(9408..10451)
                     /locus_tag="PKL93_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71319.1"
                     /translation="MFEGIANLIEKAGWPRIIIALFLLSLFVIAPFVNISIGTSISDT
                     LVRFAMNSVLVLSLVPMVQSGCGLNFGMQLGVIAGLIGAVTSIELGVTGLAGFLTAIG
                     IAIPFAAILGFFYGLLLNRVKGDEMVVATYVGFSSVAFMSMMWLLLPYKSPNMIWGYG
                     GSGLRTTISVEGYWLGVLNNFLSIRIGNFFFPTGTILFFALIALLVWSFFKTKLGTAM
                     TAAGSNPVYAKAAGVNVDRMRILSVVISTVIAAVGILVYEQSFGFIQLYMAPLLMAFP
                     AVAALLLGGASVSKASMGNVIIGAFLFQGILTMTPSVMNNLIKSDMSEVIRIIVSNGM
                     IVYALTRRTKVRK"
     gene            complement(10444..12048)
                     /locus_tag="PKL93_08415"
     CDS             complement(10444..12048)
                     /locus_tag="PKL93_08415"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNQ71320.1"
                     /translation="MDEKQYLLKMENISKEFFGNQVLKDVTLSVGKGEIVGLVGENGA
                     GKSTLMNILFGLPVIQETGGFQGRILLDDVEVNFTSPFEAIEAGIGMVHQEFILIPGF
                     TATENILLNRESTNYNIFVEVFGERLRTLNREEMHKRAVAAIEKLEVQLDPDMLVSEM
                     PVGHRQFTEIAREMDRKSTKLLVLDEPTAVLTESEAETMLTAARRLANKGLSIIFISH
                     RLSEVLKIADRLVVLRDGQVVRELESSKTTPREVASLMVGREIKDSSSVRENKSEEPE
                     IVLDIKDLWVDMPGEQVNGVNLQVHKGEILGIGGLAGQGKLGIANGVMGLFPAEGEVY
                     YKGERLPLNNPVGVLSRGIAFVSEDRRGVGLLLDEPIDLNIVFTAIQIQGRFIKNLLG
                     GLIKWRDDKEISRVAREYVESLQIKCVSEKQQAKELSGGNQQKVCLAKAFVLEPDLLF
                     VSEPTRGIDVGAKSVVLETLRTQNREHGTTIIMTSSELEELRSICDRIAIVSEGRILG
                     ILPPTADPADFGLLMLGEQEEVKESV"
     gene            complement(12124..13299)
                     /locus_tag="PKL93_08420"
     CDS             complement(12124..13299)
                     /locus_tag="PKL93_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HNQ71321.1"
                     /translation="MRKALLVLLVVLVSAAFMFAAEFHIGVVTGTVSQSEDDLRGAEA
                     LIKKYGDAASGGMIVHLTYPDNFMSEQETVIAQITGLADDPLMKAIIVNQAIPGTVES
                     FRRIKETRPDILLLAGLPHEDPPMLADAADLSVNADSLARGYLIIYTAKQLGAEKFMH
                     ISFPRHMSYELLSKRRDIMRAACEELGLEFIDMGSPDPVSDVGIAGAQQFILEKVPAW
                     LDEYGPKTAFFCTNDAHTEPLLKRVAELGGYFIEADLPSPLMGYPGALGISFTEEETG
                     NWPAILAKVEEAVVAKGGAGRMGTWAYSLGYTTTAALAEHAKNVIEGKCEIDDFDAIM
                     AAFAEYTPGAAWNGSYYVDANGIEQENYLLIYQDTYIFGKGYMGITEVEVPEKYLNF"
     gene            13558..14466
                     /locus_tag="PKL93_08425"
     CDS             13558..14466
                     /locus_tag="PKL93_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNQ71322.1"
                     /translation="MSAIVSKILTFFVLIGTGFIMKKSNLADDLFTKGISRFVLYITL
                     PALIVESMNFDFSYEMLSNSVILLMAGGVLYLFLWILGIASSRVLRLTGDVRSVFRYA
                     MLFGNVAYMGYPVVELVIGKKGVFYSAVFNIWFNILTWTVGIRIMSGKTELSGKKAFL
                     NPGMISVFIGLVLFFTPLKLPVFLDEALALLGESTIPLAMIVAGIILASSKISAILKG
                     RTIIIYSLLKLCVAPMIVFLFLSFLRLPQTVEKVLIIMSAMPAAANTSIFARLFDSDF
                     ELSSRLIASSTLFSLISIPLIISFLE"
     gene            14553..15842
                     /locus_tag="PKL93_08430"
     CDS             14553..15842
                     /locus_tag="PKL93_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ71323.1"
                     /translation="MRRLAVAFFILAPLIVLSGSVALVLSGGGAKGGYEIGAWKALID
                     LGVDLGGVYGTSVGSLNAAAVAQGDFDKALDVWRNISEESVMKPTEAQRKLIEAYGGG
                     SDWSLGELYQGAVDVINEGIDVTPLKELLSSVISEEAVRNSSIDFGLVTFDLTDVRPE
                     MLFIEDIPQGSLVDYLMASANFPGFKTVVIDGKAFVDGGIYSNQPVEMALERGFREII
                     LVDIGRTALRDRIGKFQAYLSGAKLTHIRPRVMYGSTLDFDSEKAILQIEAGYLDTLA
                     AFGLTKGKYTYIFENRDIFKMMFDSLSPEEKNEAMKIINDSREWDDPESFYSELLSSL
                     EVIYESEDPMIDVVDELASMLEVPALDLYSVEEILQALRDAYLSKGFLDEKVSPVHLR
                     KMLSFFIFLLEKAEIPEPPTQFDLFKSSFITLRGVEE"
     gene            15848..16435
                     /locus_tag="PKL93_08435"
     CDS             15848..16435
                     /locus_tag="PKL93_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71324.1"
                     /translation="MRWMIFSLVIVFLFSGCVPMALMMGPEILPSGTLKQAVGMDLLV
                     GFEESILAPWSFQYLARMGLTEYLEGSLRVAIPSIFISAPAATVEAGAKLGVPGFRNA
                     ALLVSGGAFFGKKPSGISGDDYFIPFLRVAPMAGFYIRDWMVGAKIQLFVASEAGIVS
                     GLFLTNGKFYFELDIPFIESTSSPDGGLFGISVAF"
     gene            complement(16409..>16589)
                     /locus_tag="PKL93_08440"
     CDS             complement(16409..>16589)
                     /locus_tag="PKL93_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ71325.1"
                     /translation="QRELSDTENRLELARAYYNNIATFYNTRLERIPDNIVATLARLK
                     NCELLLQSKSDGDSK"
BASE COUNT         4208 a         3831 c         3800 g         4750 t
ORIGIN      
        1 tatttattct tcggagaacg gagaaccgtt caacggcgaa ccggtttcac ttggctcttt
       61 ccaacgccct acttgctatt gcaaaccacg ttataatcga aaaccgagat tccgaaacaa
      121 gttcgggatg acaaagggag gtaggactga gaagtcatta atgcaataat agcgatggtt
      181 atttcgaaaa cgtctaaatt cgtcatgcag cggaaatggt gcaccaactt ttgaaaaata
      241 acgttccttt cgacctttca ctctcataat tttcctaatt ctatacttga caacttagat
      301 tatctgaact tgtttcagca tccagctcct atttattctt cggagaacgg agaaccgttc
      361 aacggcgaac gagttccgtt gctctttcct accacgtacc cgctaccccc aaccccggtc
      421 taacaactca caacttggga ctgatccttt gctctttcaa acccccaacc tccgaccccg
      481 aagcacgatt ttccggaacg aatctatata actccatgct gattcaaagt tagtgaatta
      541 gagcaaaagt cgaagggaaa tttccaaaag tagcacttga caatgtagat gaccagtgtt
      601 ctaatggaat agaacactat atgattaagg aggtgtgcag gtgaataaaa agtatccttc
      661 gattactaag ctaatattgg caggagtgct aggccttttg gctttgctga ttcctttcac
      721 agcgggcaag atactccttg ctattgctgg aattgttgta gttgcgttaa gcctgaagaa
      781 gttcaaatgg ctgctcataa ctctcggtat tctgttcctt gttattccga tagtagcctt
      841 gggtgtaata aatgggatcg gatcgaattg cctcaactgg cctctcgggt tcatagatga
      901 cccgtcgatt cttcagtggt ttaactggat cgaaaacgat ggttacgaat actatggcaa
      961 taacgaaggc ggctacaaca ggaaaggctc agatcataag acttatatgc ctgatcagta
     1021 tgtggatgga gcaaaggaaa tcgttttttc tggacttggt ttcgagatta gcttcgatga
     1081 aaactcagat aagatccaca tcccatcgaa gctcgaaatc aacagatatg gagaaagatt
     1141 gcagatctcg atgcccaatg atatgaaaaa ctcaactgcc caaattacta ttggaacgct
     1201 ttcagaactc gatagattgg agtttagaac ggttttcttg aggctgaacg acaaggtgaa
     1261 tgttgcgaag atggaaattc agactatctc aggagagatc aggggagaaa tcatggcgag
     1321 caagatgatt tcgattgagg gaacatctct ctcaataact ggaagcctaa aagctccaga
     1381 aatcattatt gagaatgtac cttcgctgtc cttcatggga agtgtagagg cagaaaagtt
     1441 taccgtgcag aacggggtag cactggcctt ttctatggat gcgtcgaaaa tcgagaatct
     1501 ttcgatacat ggaacggtgg tgaacgggag gataaaattc agcgattcgt ggaccggaag
     1561 cagacgggtg aacgtttcgg gaataggcgg gtcgctcaat ctattgatgc ccaccgggag
     1621 tggtggcttt gaacttatta cctctggtag ggtgagcgtc gttactgaga agtattgaaa
     1681 gaagtgatgt catgtttgga aaaatcgata aacacgatgg tgttccggcc tatatacaga
     1741 ttatgaacat agttaagagg gagataatgc tcggacgtct agaaaacgga gaccaattgc
     1801 ccccggttag ggagatggcc gcgatgttcg gagtgaatat gaacaccgta atcagatcac
     1861 tcgaaaggct gcagttcgaa gggttagtgg aggcagaaca tggggtaggc tattttgtga
     1921 agacggcaca ttcgataaac agagaagtaa tgaagacgat atctgaaacc gtaggggaat
     1981 tgaagaaaaa cggaatagat ctgcaaatgg ctaaggtttt gcttgaggag gtatggaaga
     2041 atgactgaaa agattgaagc ttattttatg aaagagttca gtgagaaggc aggaaggatt
     2101 gctgtctttt cccttggatt gctgattccc ttctatccgg tgagaatgat attcctcttt
     2161 ctattggcaa cgagtcttct cccatcagat attcagcata agaggcttgg gtcgctgctg
     2221 gcgcttccgt actcgcgcca ggagttgttc tggatatcat atctctttct cattgcgttg
     2281 agcgttgtta cacaggtgat tggaatggcg ttattcggcg ctagcctaat ctcatcgatg
     2341 aatatttact ggctttcaat ggtgatcggt tccataagtt tttctactgc ctattttgcc
     2401 gtttcaatga taagcgtaac ggccggtctg gacaactttg gtattccatt cctcgtgctg
     2461 attgccgatt taatagctgg gggtgtcgga aacaggttca gcaatccgta cttctatgcc
     2521 agcccggccc accagggtaa cgtttggatc agtggggccg tatcactagc tgtcttgctg
     2581 gttgcaaact atctattcgt caagaaagga gtgcagaaat gatagtctct gtagataaac
     2641 taagaaagtc cttctcgtcg aagcctgttc ttaacgatat ttctttcaat gtggagcagg
     2701 gagatatctt tgcgatagtc ggaccaaatg gggcaggaaa gaccaccact ctccgctgta
     2761 tattcggcga aatcgcaccg gaaggtggag aagtgaaggt ctttggggaa aatgttacat
     2821 cgcgtactaa ggagaagata gcggtcatga cggaagaccg ccttaccttc aggaggttcc
     2881 tcggcgagga ttacctgagg atgtggagaa tgctttatcc tggctggcaa gagaaggttt
     2941 tcgccagttt cgcagtgcat tacaagttca atctaaacga gagagttgaa acatactcta
     3001 tgggcatgaa aactctattc catatagctc ttacggttgc tagcggagct gatctcctca
     3061 tcctcgacga acctactcag agtctcgatc cggtgataag acaggaagtg atgggtgtgc
     3121 tgagagatta tgttctccag gagggcaaaa caatgataat ctcctctcac gaaatctatg
     3181 atctcgagga gattgccggt tcctttgccg tagtccttga gggaaaggtg ctatattcag
     3241 acacaattga tggcgccaag gagacacatc ggattattcc aaaagggcag gctatacctt
     3301 caggagagat tattggcgtt acgggtgagg aaaccctggt ccgaactgac gaagaggcag
     3361 gcagattccc gaacttcaaa gagatcgtgc tgggctacct tcagagtaag aaagcctttg
     3421 cgcctttcaa agaagagttc agaccgagat tcaagatttg agaagcgtct cgtgagtaaa
     3481 tattgcacgg ggcgagcggc agtatcgccc cgttttctga agcagaaatc gatataggca
     3541 aagacacatt tgcccaggta tgcgattcca ttattcaaag gttttcggtc attcgtcact
     3601 gagatgtttg gaatctgatt tgttggactg aatgactgga tgcgttccgt tggaattttt
     3661 accctcatag tccagtttgt atcttagtag ttcgccttcg ggactgcact ttgctctttg
     3721 tctgctgtga agcgtaaatt cgaggaagaa tctaactctt ctgtgactct tgagctgcag
     3781 aggtgtaaca tccaggatta tcaggtatta tatttgtgag gggatttcgt aagggaggtc
     3841 tttgtatgga agaagagaat gtgaagaaag agactgtaaa gaaggggagc ggactgtctc
     3901 agatctttct gggtctgatt gttattgcgc taggattggt gatcattttt gtgcccgccg
     3961 tactgtcgaa cttctggctc ttgtttatct ggcttccagg agtgataatg gaatacaatg
     4021 cttttagaaa caggaataac ggcttgcttg ttcccggtgg aattcttctg gtcgttgcgc
     4081 ttgcgctcac tcttggagtc atcttccccg gtttctattc tcaaggaggt tggggtctct
     4141 tcgttcttgc tcctgccttt gggctgctac agctctattt tgccgatgga cgaaggaatt
     4201 tcggtttact cgttccgatt gcgatattaa ctgctgtggg tgtgatcgct cttatacctt
     4261 catcactttc ccaatggaca atgatagtgg ttggggcggt tcttattgtc tctggtttgc
     4321 tcgttctttt tagaaggaaa tagtcaaacg tctcgtccca tctgatagaa atcgaagtaa
     4381 tcatcgttga ctcgcatgaa tttcttcagt ttcccttcga tttcgaagcc catctttctg
     4441 tagagagcta tcgcccgatc gttatcggta cggactttaa gaccgatcct tgtaatcccg
     4501 tttctgccag catgttcaac tgcgagttca ataaggactc ttcctattcc cattccccag
     4561 tacttcattg acactgaaag agctatttcg gcccagtggt atattcgctt tcttgagccc
     4621 ccttgcattg tgataagacc tactatgctc tgatcacatt tggctactgc catgagacag
     4681 ttgcttctct ctttgaaaga acttatgaga cgcctttcat cctcactgct catggtgaac
     4741 tcgcctcttc caaagctgag aaagtcggac tcgacagcta ctgaattgat gtattcgatt
     4801 attctatctg aatcgccgat gtctgctctg tagaagtgac aagaatcacc gtttttgaga
     4861 gtgatttcgc gcagagggct ctcagaatgg tagttttcca tcttcgcctt ccgtggatct
     4921 ctcttgaggc tcctcggaaa tctcttccga ctcaattgaa ttcagaaaag tagagtactg
     4981 ttgatcaagt tcttctttct ctttcttgag agtgatcaaa cgcaatcggg cggctgtaag
     5041 cagctgaata gccttctggt aagctttgag tgcaatttcg acgtcgatct gagagttgcc
     5101 acggaaatac tcgtctatcg atcttatgta gccaagaagc ttcttgaagt ccatagcgtt
     5161 tatctgatct tctttcagtt tcagaattga ttcgagagtc ttttcatcca tttcatacct
     5221 ccgatttcac ttcatcaatt gtagcttttg cttcgccgtc tttgaaaaca cagtcaactt
     5281 catctccggg agagacaatt ctgacactgt taacaatcct gtcggctttc ttcaggacca
     5341 caccaccgaa aaggagcgag gctgcgtatc ccccatgcaa agtgaggtcg ttgaagaggt
     5401 tgtcgaggtc gttacctgcc tcgttgattt tgtcgtcgat cagtctgaat attcctttgt
     5461 caagtagctc gacctgtccg tttgcgttca tcaaatgtct gtcgagctga tttctcaatc
     5521 ctgcataatc tagctgactt tcgctgtagt gaaaacttct ttggatggtc gtctccgccc
     5581 tgtttattag gccctcggta agtgctaaaa tttcgttcat cgttcgctca atcgagagaa
     5641 catacctggt tgggttgaag gatccaagtt cgtttacagc ctggccgatc ctgtgaagaa
     5701 tggttcccga caaaaccctt atctcatcct cgaagaaatg ctccgttcga ctcatatttt
     5761 cctctattct gtccgacatg atcccaagtc ttttaggatc ggtctcatcc caagctcttt
     5821 ggaatagcca gttcatttcg tcgtctaaga gatccgcgaa ttcttcaaga gaggcgatat
     5881 actcgtttac ctgttcggtt attcctctcg ctactgccgt tggggtgtcg aatctcttcc
     5941 atgcgacgaa gtccggaatc gtgaaatctc tttcatggcc gatagcgcta agcacagggc
     6001 agtaagtctt gttgaaatcc gaaattgcac ttcccaaatc caggttgtcg aagtacatta
     6061 agtcgctcgc agagccacca cctctggtca ggaccacaac gtcgtattta acgggacagg
     6121 aggcgatttt cttcaaagcg gaaagaatcc cggggacggt tcgattcccc tgcatatatg
     6181 attcaaaaag atgaacaata ggtttgtaag gatattgaac agagagattg gagaagaaat
     6241 ccccgagtcc cgctgcagtt gaagacgaga tgacggcaat cctcttaatc gggggcagct
     6301 cggcgagatc gtgttcctcg gttctcaatg cattccgagc tttaagagcg agatagatct
     6361 cccttcttcg agcggcaata tccgactcac cgatcggcgc gatcgtatct atccagatgg
     6421 aaaagcttgc acgcggtttg tagaaactga gagtaccttc gaagagccac ttcttacctt
     6481 tcaagtcgga cgagctcttt agagctagct gtcttcccat ccttgcaaag gcagaggtgc
     6541 ctgaaatcat gctcagatct attcgccgct tcgtacctcg ctcaacgtaa tcctgagata
     6601 cattgatata tacatagctg cctctgacgt ttatattggt aacatcggca ggaaacttga
     6661 ctctcagatt gttcaaaccc gcatctgcaa gaatcgttct tatatgttct agcatattgt
     6721 caagatcctt gaattcaaga gtttcttctt ccatgaagtt ctcctctgaa tgaatgctct
     6781 actccattat aaaagaagag gacgcctaat ggcgccctca gtaggaacat tcacaattat
     6841 ttcgccgtat agccaccgtc taccatcaaa gactctccgt gaacaaaaga ggcgtcctca
     6901 ccggaaagaa acaagacggc attagcgact tctctgggat ttcctagcct gcctatagga
     6961 tgcaagccaa ctagcatgtc gtacatctcc gttccctcct tcattccggc attctcaatc
     7021 agcggcgtct ttatgaaagc aggattaacg gcattgattc ttatgttttt gcttgcatac
     7081 tccagcgctg cggctttcgt caatccaacg acagcgtgtt tggcggctac ataagcggag
     7141 gcattgttga aaccgacttt tccaagtatt gaagagttgt ttacaattgc tcctccgccc
     7201 tgcttaagca tctgctgaat ctggtacttc attccataat agacgcccag caaattgatc
     7261 gaaatcactc gttcccagtc ctcttcaggg tactcagata tcggaagaga agggccagca
     7321 atcccagcgt tattgaatgc gacatcaagc tttccaaact tctctacgat tttgttgatt
     7381 atttcctttg tctgcttggc gtctgcgaca ttgtgcttca tgaacactgc agtgccgcca
     7441 gattcgttta tctcttcggc taacttcctt cctttttctt catcgacatc tgtaattact
     7501 acctttgctc cctcttctgc gaatgcgatg gcggtttcgg ctccgattcc agagcttcct
     7561 cccgatatca ttactacctt gccttcgaat ctcttcatag tgaacctcct tatataagga
     7621 tgaaatccga tagtttctat ctgctatatt tagtatatca caatccttgc taagctcaaa
     7681 taacgatcca gactgcgctt ttaagaggag ctttcaaaaa tcgattgacg atgattcgct
     7741 tatcaacttg tgaaaggctt ggcctagaag aacgacgctt tattcgaagt tttttcgatg
     7801 caaaaagaaa agggaccgaa cgctgacatc attcagtccc tttatcatca attgcggtta
     7861 gctcaaccat tcatttcgag atttcatcgc tcggcttgct ctatcgtaag ccatccctct
     7921 tcgacttcaa aaagcgcgac aagatttatt actgcagtat cgtttggcga caaagtaaac
     7981 tttcgttcaa tttcccttgt agtcccatca ggaaacactt caactgcctt tattctgtgc
     8041 cacggaccgg ctgaataagc aacctttctc tttccctttt tgacatcctc aagttcgata
     8101 tcgtcgacaa ttacatttat tgtatttgcc gatttatatt cgacgccgtc tacgacgatg
     8161 tctttgtttt ctacgaagat ggtatgttcc tttccgagaa cgtaaagacc aaatccaaga
     8221 gcaacaagga ccacaacaag ggcgactcta ataatgttcc tcctcatgcg atcagcctcc
     8281 tgtcccatct tcaaccgctg ctttgattgc tgcctttgcc tgccttcttt cggtctcctt
     8341 ttcaacctgc cttctccagg cgtagagagc gagtgagatg gcgattattc cataagatac
     8401 aaagaccctg aaaaactcgc cgatctgtgc ctggcctaag agttcttttc ccgctcttgg
     8461 agcgacaata aaggtcagat ggaagagagt gattccaatg aagacatttg gaatggttgc
     8521 ttttgcgact gaggcgcctc caataagaag cgcggcaatg gcgaacgttc cgacctgatc
     8581 gtgggaatta tatgtgttga tcgtcccgat attctgcagg aagataatct gaccgtaaca
     8641 ggcgagaacc gtcgaaataa ttatcgagag cgttctcgtc ctatccacgt ttattccggc
     8701 ggttgccgcc acttgcatat cctggcccac agccctcatg tcttgaccga gctttgtcct
     8761 cgtgaaccag aaaacaaata tacagagtgc agctattatt gcgaagacac ctagaggaat
     8821 agagaccccg cttatttcta tggaccacat attgtcgaga gcttttctta tgcttattaa
     8881 atcgattgca tttctgacac cgtatcctct gggaagcagc aacttgtcac tggcaatcgg
     8941 tattacactt cccataccgt agaggatcgt aagctgataa atcccgttta tgaagaatcc
     9001 caaaatcatt gatgtaacca tctctctgcc ttttgcacga ttgaggatca ttcccgcaag
     9061 catgcccaaa agaatcgcga ttggtgttcc aataagcata gcaagaacaa ttcccggaat
     9121 acctactact ccccagtcga gcatgaaaat caagcctatc tgccctgcca tggctccaag
     9181 cacaataccg aagttcaatc ccatgccggc cataacaggt atcagtagag atatgacaag
     9241 gaaggagttt ctcccgagtc tcgtgatgat ttcgttcaga agataatcag ttgagaatcc
     9301 cgacaacggc acggcgaaaa taaggagaat gacaaacaag attggaaccg cattggaaat
     9361 tacgaatctc accaatggat tctttgcgcg tgttaagctc tgatccttca ttttctcacc
     9421 ttcgttcttc tcgtcaaagc ataaacgatc ataccgttag agacgattat cctgattacc
     9481 tccgacatgt cgctcttaat gagattattc atgacggaag gtgtcatagt tagaattccc
     9541 tggaacaaga acgctccaat gataacgttt cccatagacg ccttcgaaac ggatgcgccc
     9601 ccgagcagta aagcagcaac ggccggaaag gccatcaata gaggggccat gtataactga
     9661 atgaatccga aactctgttc atagacaagg attcccacag ccgcaataac cgtagagatc
     9721 acgactgata gaattctcat tctgtctaca ttaactccgg ctgcttttgc atatacagga
     9781 tttgatcctg ccgccgtcat cgccgttcct agcttcgtct tgaagaatga ccagacgagc
     9841 aaagcaatga gggcgaagaa aaggatcgtc cccgttggaa agaaaaagtt tccgattctt
     9901 atgctgagaa agttgttaag aacacccaac cagtatccct cgacacttat ggttgtcctt
     9961 agaccggagc ctccatatcc ccatatcatg ttcgggctct tatatggcag gagaagccac
    10021 atcatactca tgaaggccac tgaagagaaa cccacgtatg ttgctacgac catctcgtct
    10081 cccttaactc tgtttagcaa aaggccgtag aagaacccta gaattgctgc gaatggtatt
    10141 gcaataccaa ttgctgtcaa gaaaccggca agccccgtta caccgagttc aatgcttgta
    10201 accgcaccga tcaatcctgc aattacaccg agctgcattc cgaaattcaa tccgcaaccg
    10261 gattgaacca tcggtacgag cgaaagaacc agaacggagt tcatggcaaa ccttactaat
    10321 gtgtcgctaa tcgacgtccc tatacttatg ttgacaaatg gcgctattac gaaaagagat
    10381 aaaaggaaga gagcaattat gattctcggc caaccggcct tctctataag gttcgcaatg
    10441 ccttcaaaca ctttccttca cctcttcctg ctcgcccagc ataagcaaac cgaagtcagc
    10501 cggatcggcg gtcggcggta gaattccaag tatccttcct tcacttacta ttgctattct
    10561 gtcgcagatc gagcgaagct cctctagctc ggaggaggtc ataatgattg ttgtgccgtg
    10621 ttctcgattt tgtgttctta gggtttcgag aaccacgctc ttcgcgccta cgtcaattcc
    10681 cctcgttggt tcagagacaa acaagagatc gggttcgaga acgaaggcct tggcaagaca
    10741 taccttctgt tgatttcctc ctgaaagctc cttcgcctgc tgtttttcac tcacgcattt
    10801 gatctggaga gattcaacgt attctctggc cacccttgag atttctttgt catctcgcca
    10861 cttgatcaag ccacctagaa gattcttgat gaaccttcct tgaatctgga tcgcggtgaa
    10921 aacaatattc aaatctatcg gttcatcaag gaggagacca acgccccttc tgtcttccga
    10981 aacgaatgcg atgcctctac tcaggacgcc gacgggattg tttagcggta atctctcgcc
    11041 tttgtaatag acttcacctt ccgcgggaaa gagtcccatt actccgttgg ctattccaag
    11101 ctttccttgg ccggcgagac ctcctatgcc gagaatctct cctttgtgaa cctggagatt
    11161 gactccattt acctgttctc cgggcatgtc tacccagaga tcctttatgt ccagaacaat
    11221 ttcaggctct tcacttttgt tttctctaac cgatgagctg tcctttattt ctcttcctac
    11281 catcaaagag gcgacctctc ttggagtcgt ctttgaggat tccagctctc gaacaacctg
    11341 accgtccctc agtactacta gacgatctgc tattttcaac acttcgctca acctgtgact
    11401 aatgaagatt atagaaagtc ccttatttgc aagtcttctt gccgccgtta gcatggtttc
    11461 cgcttcggat tccgttagga cggccgtcgg ctcatcgaga acgaggagct tcgtagactt
    11521 tctatccatc tctctcgcga tctcggtgaa ctgccggtga ccgacaggca tttcgctaac
    11581 gagcatatca gggtccagct gtacctcaag cttttctatt gcagcaacgg ctcttttgtg
    11641 catttcttct ctgtttagag tacgtagtct ctcgccgaag acctctacga aaatgttgta
    11701 gtttgtggac tctctattca gcaggatgtt ctccgttgcc gtgaagccgg gtataagtat
    11761 gaactcctga tgtaccatgc caatccctgc ttctatcgct tcaaagggac tggtaaaatt
    11821 gacttcgacg tcatctagaa gtatccttcc ttgaaaaccc ccagtttcct gaatcacagg
    11881 caggccgaag agaatgttca tgagcgttga ctttccggcg ccgttttctc caactaatcc
    11941 aacgatctcg ccttttccaa cactaagagt tacgtctttc aaaacctgat taccaaagaa
    12001 ctctttactg atattttcca tcttcagcaa gtattgcttt tcatccatgg cttcacccca
    12061 aataccgaac gttggaaaga atcaagaaaa ggggggctta ccccccttct tgacgctatt
    12121 agcttagaag tttagatatt tctcaggaac ttctacttcc gtgattccca tgtaaccttt
    12181 gccaaatatg taggtatcct gatagatcag gaggtagttc tcttgctcga ttccattggc
    12241 atcgacgtaa tagcttccat tccaagccgc gcctggtgtg tattcggcaa atgccgccat
    12301 aatggcatcg aagtcgtcaa tctcacattt gccttcgatg acattcttgg catgttctgc
    12361 aagagcggct gtagtcgtgt aaccaagaga ataagcccat gtacccattc tccctgcccc
    12421 acctttcgcg acaacggctt cttctacctt tgcgagtatt gccggccagt tgccagtctc
    12481 ttcctcagtg aaggagatac caagtgcgcc aggatagccc atgagaggag aaggaagatc
    12541 ggcttcgatg aagtaaccgc caagttcggc aactctcttt agaagaggtt ctgtgtgggc
    12601 atcgtttgta cagaagaagg cggtctttgg tccatactca tcaagccagg cagggacttt
    12661 ttcaagaatg aactgttgtg ctcctgcgat tccaacgtca ctgacagggt caggagaacc
    12721 catatcaatg aactcgaggc cgagttcttc acatgcagct ctcatgatgt ctcttcgctt
    12781 ggaaagaagt tcgtaagaca tgtgccttgg gaatgagata tgcatgaact tctctgcgcc
    12841 cagctgcttg gccgtgtaga ttatcaggta gccacgtgcc agtgagtcgg cgttgacgga
    12901 aagatcagct gcatcggcta gcattggagg atcttcgtga ggcagaccgg caagaagaag
    12961 aatgtctggt cttgtttcct tgattcttct gaagctctcg actgttccag gaattgcctg
    13021 gttgacaatg atagccttca ttagaggatc gtctgcgaga ccagttatct gtgctataac
    13081 tgtctcttgt tcagacatga agttatcagg gtaagtaaga tgtacgatca tcccaccgga
    13141 agcagcatcc ccgtatttct tgatcagcgc ttcggcgcct cgaagatcat cttcagactg
    13201 tgacaccgtt ccggttacaa cgccgatgtg aaattctgcg gcaaacatga aagccgcgct
    13261 tacaagaact accagcagaa ctaatagtgc tttacgcatt caaaaacccc cttttattaa
    13321 ggatttaggc tgcccgacag gcatccttta ataacatatc ttgtttgacc gatataaaag
    13381 ccggatggcc cggatatcaa aaaggaaagt ataatgttta acgagactag tttatcacaa
    13441 ttttgagtca ttaatgtaat gacctggttc ttcagtattg gtcattcaag ctccctaagt
    13501 acgtctagtc tcctctcttt gttctgtcct ttgttatgct ggtttataat cctcttgatg
    13561 agcgcaatcg tctcgaaaat cctaactttc tttgtattga taggaactgg attcataatg
    13621 aagaaaagta atttggccga cgatctattc acaaagggga tctcaagatt tgtcctgtac
    13681 ataaccctac ccgctttgat agtagagtct atgaatttcg acttttctta cgagatgctt
    13741 tcaaactccg taatattgtt aatggccggt ggtgtgctct atttgtttct ctggatcctg
    13801 ggaattgctt cttccagagt tctcaggctt accggtgatg ttagaagtgt atttcgttat
    13861 gctatgctct tcggaaacgt cgcctatatg ggctatccgg ttgtggagtt ggtgatcgga
    13921 aagaaaggtg ttttttacag tgcagtgttt aatatctggt tcaacatatt aacctggacc
    13981 gttgggatca ggataatgtc gggcaagacg gaactgtcag ggaaaaaagc cttcctgaat
    14041 cccggaatga tctcggtctt cattggcctt gttctcttct tcacacctct taaacttccg
    14101 gtctttctcg acgaagctct tgcgcttctt ggggagagta ctattccatt ggctatgatc
    14161 gtagccggta tcattttggc aagttcaaag atttctgcga ttctgaaagg gcgaacgata
    14221 ataatctaca gccttttgaa actttgcgtt gcgccgatga tagtctttct tttcctttcc
    14281 ttcttgagac tgccgcaaac ggtcgagaaa gtgctgataa taatgagtgc aatgccggct
    14341 gcagctaata cgtcgatctt tgcgcggctt tttgattcgg acttcgagct ttcttcaaga
    14401 ttgatagctt cctcaactct tttctcccta atttcgatcc ctttaatcat ctcttttctt
    14461 gaatgaaaag gtcagatcta gattttctct tccgttttgc tttgagataa tgctatcatc
    14521 cctctataaa ctcccgaaga gaggtgtgaa aattgagacg tctcgcggtt gcttttttta
    14581 ttttggctcc tttgattgtg ttgtcgggaa gtgttgccct ggttctttca ggcggtggag
    14641 cgaagggcgg ctatgagatc ggtgcctgga aggctttgat agatctcgga gtcgatttgg
    14701 gtggagtgta cggtacttcg gtaggttctc ttaatgccgc tgcagttgca caaggcgatt
    14761 tcgataaagc attggatgtc tggagaaaca tttctgaaga gagcgtgatg aagccaacgg
    14821 aagcgcagcg aaagttgatc gaggcatatg gaggaggttc tgactggagt ctcggtgagc
    14881 tttatcaggg agctgtagac gtcatcaatg aaggcataga tgtgacgccg ctcaaggagc
    14941 tgctcagctc tgtgatatcc gaagaagccg tgagaaactc ctcgatagac ttcggacttg
    15001 tgacttttga tctgaccgat gtcaggcccg aaatgctctt cattgaagac atacctcaag
    15061 gatctctagt ggattacttg atggcaagcg cgaatttccc cggattcaaa acggtagtca
    15121 tcgatggaaa ggcctttgtc gatggaggaa tctactcaaa tcagccagtt gagatggctt
    15181 tggaaagagg ttttagagag atcattctgg tcgatatcgg tagaactgct cttagagata
    15241 gaatagggaa gttccaggcg tatctttccg gggctaagct cacccatata agaccgagag
    15301 ttatgtacgg cagtactctg gatttcgatt cggagaaagc catactacag atcgaagcag
    15361 gctatctaga taccctggcc gcttttggct tgacgaaggg aaagtacaca tatatcttcg
    15421 agaacagaga catcttcaag atgatgttcg attcgctctc cccggaagag aagaacgagg
    15481 caatgaagat aatcaatgat tctcgggaat gggacgatcc cgagagcttc tattctgagc
    15541 tcttatcatc tcttgaagtg atttatgaga gtgaggatcc catgatagac gtagtagatg
    15601 agctggcttc aatgcttgag gtaccggctc ttgatctgta cagcgtcgaa gaaatactcc
    15661 aagccctgcg tgatgcctac ctgagcaaag gattcctaga tgagaaagtc tcacctgttc
    15721 acctcaggaa gatgctaagc ttttttatct tcctattgga aaaggcagag attcctgaac
    15781 cacccactca attcgacctt ttcaaatctt ccttcataac gcttagggga gtagaggagt
    15841 gaatactatg agatggatga ttttttctct ggtaattgtg tttctgttct ccggttgcgt
    15901 tccgatggct ttaatgatgg gaccggagat tttacctagc ggcacgttga aacaggccgt
    15961 tggaatggat ctccttgtag gtttcgaaga atctatcctc gccccctgga gtttccagta
    16021 tcttgccagg atgggcctta cggagtatct cgaaggttca cttagggtag caattccgag
    16081 catcttcatt tccgcgccgg cagccacggt cgaagctggg gcaaagctgg gagtaccggg
    16141 cttcagaaac gcagcccttc tggtgagtgg aggggccttt ttcggcaaaa aaccaagtgg
    16201 aataagcgga gacgactact tcataccatt cctcagggta gcgccgatgg cggggtttta
    16261 catacgagac tggatggttg gggctaaaat ccagcttttc gtggccagcg aagctggtat
    16321 cgtttccggt ctcttcttga caaatggaaa gttctatttc gagctggaca taccttttat
    16381 cgaaagcaca tccagccccg atggaggcct atttggaatc tccgtcgctt tttgattgaa
    16441 gaagtaactc acagttcttc aaacgagcca gagtagcgac tatattgtcg gggattctct
    16501 cgagccttgt gttgtaaaat gtcgcgatgt tattgtaata ggctctggcg agttcgagcc
    16561 tattttcggt atccgatagt tctctttga
//
LOCUS       DAOQYI010000034        16522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_3927_length_16522_cov_73.222141, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000034 DAOQYI010000000
VERSION     DAOQYI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 16522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16522
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3927_length_16522_cov_73.222141"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(964..1641)
                     /locus_tag="PKL93_08445"
     CDS             complement(964..1641)
                     /locus_tag="PKL93_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71326.1"
                     /translation="MSREMRIMWLHNRLLKNDFAAMKDYTQKFGISVRQAHRDFKYLR
                     ANLGAPMKYSRKRGEYFYSEPYHLPSLFEDSMKFQLRTEYRISSVFLNAIASKKAVKI
                     FQRGGKEFIFYPACFDERRELFCGLQEDGNVRFVRSDEIDKVIFSNKRYLEEPMLWNR
                     IFPREAEFHEVDLDFGGDRHKYHFFEIGDLVMFLASENSFKVIGPQEIIVELRKVAKN
                     LLKTIGD"
     gene            1768..2430
                     /locus_tag="PKL93_08450"
     CDS             1768..2430
                     /locus_tag="PKL93_08450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ71327.1"
                     /translation="MMVRGIIFDLFGTIVSNARLFRPVCSKMAQESSAEVVEIERDFV
                     NLYGHYFKDCHKMPFQPERYYYYLLISDLIDKYDLPGDMEAYCNFMYDSFSKFRAYPD
                     ARILRAIVNKYSVAILTNADNSFVEKVVKRNRLPHHFLLTSESARSYKPSEVIFERLL
                     SMMEIEKRHAVFVGDSIQADMLGASAAGIKGILIDRSRSYLDYAPRIESLEELPSLLE
                     SL"
     gene            complement(2408..3445)
                     /gene="ltaE"
                     /locus_tag="PKL93_08455"
     CDS             complement(2408..3445)
                     /gene="ltaE"
                     /locus_tag="PKL93_08455"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNQ71328.1"
                     /translation="MKWIDIRSDTVTVPGEEMRRVMASAEVGDDVYGDDPTVNRLEEM
                     SADILKKEAALFVPSGTFGNQLSILTHTLRGDEVIIPASNHIIVHEAGASAVIAGVQM
                     RTLDCDDGKPSAERIRSAIRSEDLHYPRTGLICLENAHSSGRILPMNYIREIYELASA
                     QKVPLHLDGARIFNAAIAEGVSPAEIASCSDSVMFCLSKGLGAPIGSMLVGKREFIAK
                     ARKGRKIMGGAMRQAGIIAAAGVFALERMIDRLQIDHDNAKYLAEGLSKLPNIEVFFD
                     RLDINMVFFRITGKASSKFIVETLHDKGIKINPPEAGEWRFVTNLNVTREDLDIVLEE
                     FEAALTRTLAS"
     gene            complement(4043..4174)
                     /locus_tag="PKL93_08460"
     CDS             complement(4043..4174)
                     /locus_tag="PKL93_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71329.1"
                     /translation="MTMIAFCGIGCTQCDTYRVTVADDNSLRRETLNGWGGRRILVI"
     gene            4263..5000
                     /locus_tag="PKL93_08465"
     CDS             4263..5000
                     /locus_tag="PKL93_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNQ71330.1"
                     /translation="MKPVVTKEVYRVRYYELDCQWKASISSLMDYFNDVVTLQTVEMG
                     HGVDVMSKGEYAWLLLRWDVKVNRYPDYLENVVVQTIPYSMDRFYAYRRFEIFDCSGN
                     VIVDANSQWILIDQRKRRPIRIGDQFYELYGIDSDFHEPLSFPKVNENESSSEEITFI
                     VRNSDLDTNGHSNNVAYVRWIMETVPSEFVKRFLKRLTIEYKRESRKGDVISIESVFE
                     NGAEFAEGKHKITSSGRVLSLARTEWK"
     gene            complement(5021..6124)
                     /locus_tag="PKL93_08470"
     CDS             complement(5021..6124)
                     /locus_tag="PKL93_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNQ71331.1"
                     /translation="MIDLQKPTYLTSVEKIEELNDEQISEMKKVTDVYPFRANDYYLG
                     LINWNDPHDPIKRIILPDFEELDEWGDLDASQEHKYTVAPGMEHKYKDTALLLVSKVC
                     GSFCRFCFRKRLFSTENKEVVNDVTLGVEYIRKHKEITNVLLTGGDSLILSTEKLGDI
                     VRQLREIDHVGIIRFGSKMVAFNPYRIINDPDLPEMVKKYSTPKKRIYIMAHFNHPRE
                     LTDEAIRGLNILRDAGAVICNQTPMIRGVNDSVDVMTALFRKLSFIGIPPYYVFQCRP
                     TKGNHTYAVPAEKGYEIFKKSIDSVSGLAKRARFVMSHATGKIEYVGLDEKKIYMKYH
                     RAADPKRYDLFMAFDRNPDAYWLDDYVNPDSLV"
     gene            complement(6488..9490)
                     /locus_tag="PKL93_08475"
     CDS             complement(6488..9490)
                     /locus_tag="PKL93_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNQ71332.1"
                     /translation="MKRWMIYLLIAAVAVAIVLIIALPALLNKEPKLQFPVHVVNQGE
                     KLSANLKPFVIDEKIDEVVLELVEGPGTISGFSYVFEPGFSYEGEVRVSIRATDKKGK
                     SSQQEMLINVVRVNRPPEIDTSPIVVREGETVTIDLNSLAVDPDGEELEFSVQGPGEL
                     HGSAYIYSPGYSDSGKTVLRVLAKDPNGNETARDIELEVIDVNAPPVLQLENQIVNEG
                     EVLSVDISSKVVDEDNDKVVLSLEEGPGEITEGVFTYEPSYSDSGTETVRIKASDGSG
                     NDVFSSFEILVIDVNRPPKSLLSDINVNEGESLSFDLLSRVVDPDGDNVTFSLEGPGE
                     IVDGSYIYNPDYSDAGDKSVVLILEDEKGATNTASFMVRVNDVNRPPQISIPDQSVKE
                     GEQLKIYLKGFISDPDGDEVTFEIADGPGFIEDDHYLFTPDFDSEGIHEVTLTAKDNK
                     GLESVGFFKVTVENVNRSPVTLSPSINTSIMESFTLDLDLGSLFVDPDGDDLQFEVEG
                     YGIVVDNSYQYSPGYNDKGEKVATVTATDPYGLSDSLVIRIDVKDRNRNPESSIEEMK
                     TSIHEGSSLKIDLRPLFSDPDGDELIYSLSGIGEIEGSNYSYSPGYDEAGEKNVIVTA
                     SDNNGGSLSLPIIISVIDVNRPPQISIPDQSVKEGEQLKIYLKGFISDPDGDEVTFEI
                     ADGPGFIEDDHYLFTPDFDSEGIHEVTLTAKDNKGLESVGFFKVTVENVNRSPVTLSP
                     SINTSIMESFTLDLDLGSLFVDPDGDDLQFELQGIGSISANSYIYTPGFNDSGEKTAT
                     VTAIDSNGSSQSLPIRIEVRNLNRPPDVFLPDCIAEAGKEFSLYLRAFATDPDGDALR
                     FNLISGPGEVVGDRYFFLPERADIGEREVILEVSDELGMKTKKGFRLEVKASEKVVRI
                     SYALPLFSSENVRIVAGEYEIVSDRKQAVIKTDWEFNFDEIQFYSINESEETLIGTAI
                     FDSTDDSLNRKIYSPSGDYVGNIILVTE"
     gene            complement(9572..10216)
                     /locus_tag="PKL93_08480"
     CDS             complement(9572..10216)
                     /locus_tag="PKL93_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNQ71333.1"
                     /translation="MILNVVLIALVILLAAFILMSRRKTSFRTESIIHKVERISELST
                     ARMTTLLIFEPERESLVPGTFQRFIFIVPFQVRGYVDLRKLKEDSIEIHEGEARRIKL
                     ILPSPRLEVTIPQSKIPDIRVINQSGVLTRIFGNKDMLKIFQSYSDKIEKQALQNASE
                     MGVEETSRESARNFFHGLFLGMGFDEVVVLFEQDNSLETNVEIEKAGNQRKEIN"
     gene            10767..11723
                     /locus_tag="PKL93_08485"
     CDS             10767..11723
                     /locus_tag="PKL93_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ71334.1"
                     /translation="MEYRKVGKSGLLVSELSLGSWLTFGAQLDIDNAREAMREAYRAG
                     INFFDTAEAYASGMAESMMGEILKEFKREEVVVSTKIFWGGNKPNQKGLSRKHLLEGT
                     WNSLKRLQLNYVDILFAHRPDPEVPMEEIVLGMDYIVRNGLAFYWGTSEWSSKELEEA
                     HKVADRLNAIHPVVEQPQYNMFVRERVESEYRPIYEKYGLGLTVWSPLASGLLTGKYN
                     KGIPEDSRLAKFPGLKKHLEENKLFSSENLKKVERLAAVAKKLDTELAQLALAWILKN
                     ENVSSIILGVSKVQQLRDNLKALDVKDKLTEEIVKEINSIIE"
     gene            11773..12387
                     /locus_tag="PKL93_08490"
     CDS             11773..12387
                     /locus_tag="PKL93_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71335.1"
                     /translation="MKRLLLFLSVILIIFLGSSCLYVISLPIPVGEGLIEPIEFELPG
                     SILPLSGFAFGTETVPSLDEIIQEAEKEIQLNLPDNFVITGMEIKARVEWVPENPDSE
                     NSIDIDFYVFREGHSEEEFLAFFNDPDSISDHKLFSGLIYPGENTFRLHSGESAALDM
                     ILEIINGGTAEDLDWFAIYDYEAPSDSTITIHLSGTISVKRVAE"
     gene            complement(12448..14019)
                     /gene="pruA"
                     /locus_tag="PKL93_08495"
     CDS             complement(12448..14019)
                     /gene="pruA"
                     /locus_tag="PKL93_08495"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNQ71336.1"
                     /translation="MFDVTDFQIIPPFKNEPYIDPSDPEVRSQMMLAFERIRSHERNY
                     DLFIGGRYLSTETKIVSKNPADPDEVVGTVSKATREIVDKAIDAAYFAFKSWSRMPAS
                     ERVVPFLKAAQIMRERRYELDATMILEVGKSWIEADADLAEAIDFLEYYSREALRYGQ
                     SQPVVQIPGEYNELRYIPLGVGVVIPPWNFPSAIMAGMTTAAAVSGNCVLLKPASDAP
                     VIAAKFVDILREAGLPDGVVNYVPGSGSEIGDYLVEHPLTRFVSFTGSKEVGLRINEL
                     AAKHQKGQKWIKRVILEMGGKDCVVVDETADTDAASTGAVASAFGFQGQKCSAGSRII
                     VVREVYDEMVKLVKQKTEALTIGDTTDPNIYMGPVINESAVSKILRYIELGKKESKLI
                     TGGNRLKRRGYFIEPTVFADVSPTAAIAQEEIFGPVTAIIEARDFEHAIAIANGTEYG
                     LTGAFYSKRRDRIEKAKTEIHVGNLYFNRKCTGALVGVQPFGGFNLSGTDSKAGGRDY
                     LLLFLQGKSISEKIL"
     gene            complement(14032..15174)
                     /locus_tag="PKL93_08500"
     CDS             complement(14032..15174)
                     /locus_tag="PKL93_08500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNQ71337.1"
                     /translation="MRSKYKVVIIGGGIVGTATAFYLAKNGLNDVVVIEKEYLSSGST
                     GRCGGGIRQQWSEKSNVKLAIRSVKHFKEFQKEVGFDIEYFQGGYLLLAYTDDEVSLF
                     EKNTKMQREEGLDVLMLSAKETAKRFPLIDLKGIKAAAYCPSDGHANPHLTTFAYAHA
                     AKAMGVDFSTHTTALRILTQNERITGVVTDRGKIEAEIVVNAAGGYSREVGAMAGIDL
                     PTESYKHQIFVTEPLEHMLDPLVISFENNFYIRQTKSGNFIMGQGSKDELPGHNIEPT
                     WTFISEMAKKMPGIFPFLRNVRVLRHWAGLYNMSPDARPIIDRSKDLENFFYAVGFSG
                     HGFMLAPAVGEAVAEWIIYGEPRSADLSDLSLERFEHGITKERNVV"
     gene            complement(15164..>16522)
                     /locus_tag="PKL93_08505"
     CDS             complement(15164..>16522)
                     /locus_tag="PKL93_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ71338.1"
                     /translation="VLMIGSGNIGLIVSYQLLQAGVEVRAILEASNRIGGYQVHADKV
                     RRVGIPILLQHTILRAIGEESVRGAVISKLDERWSPISGTERELVVDTICIAVGLLPS
                     IELAAQAGCEMKYVQELGGYVPLRDEDMRTSIDDIFIAGDAAGIEEATTAMIEGQIAG
                     LAVVSDLTKNSIQERLKYLKDTLRKFRSGPTSSKTRAGLSKLGSKFEEVSFTNGNQND
                     FTKYTGKMRPLIECFEAIPCNPCETSCPAGAISIGDNINSRPIIDYSKCTGCGICATT
                     CPGLAIFMFRENKDGTCSVGIPYEFLPLPAEGQYVTALGRNGEAICKAKVEKVVKSAN
                     KTNLVYISVLREYASDVRHILVPSKESGAMVCRCEEVTVDQVRKAIKEGYTDFEELRR
                     YLRIGMGPCGGRTCRLNTLMILSEETGVPVERLSPGVFRPPSIPVSFKALGEGRVDGY
                     EE"
BASE COUNT         4431 a         3775 c         3445 g         4871 t
ORIGIN      
        1 gaatcattga acttatcgcc tgagaaactc tgaaaacttc ggccccatgt cctgcgtttg
       61 gagcatagac cattgatgaa cagccgggca gttcttttat gtataagtta gctgagtcta
      121 tgggccaata tggatcattc gttccgacaa caattagctt tgaaattacg agtagagatc
      181 ggtaggtgaa agatctatgt actctagcag atctagcttt tcagggttat tcagatcatc
      241 gaggatacct ctttgtacat actcatctat ggagcgacta aagtctcccg agaactcaag
      301 ctggtttcca cctgtacagc aatattgagg ttgttaaagg ctgtgggaaa gattcctttg
      361 actctcttgt ccatcgtggc agttagccat gtggttcaac ctcgcttgga ctcccggata
      421 aaatgaagcc ttcaacttcg ccccccttgt tccctatgaa gtcattcatg atgttcactg
      481 aagcaatagt ggcttgaacc atgggcaata aggcggggca tctgtaatca caagtcttaa
      541 gacatcttac aagagaatag ctaagtaacc ggtcctcttt gagaccgcca aaaagtggtt
      601 gatttgggat gtcaaacagg acggcaacgt aaactctatt ttgcagagcc agcattccga
      661 ataatgccgg ctcttcctac gagtatccat ggtcaccggc tatgaacatc actgcagctt
      721 ttgactctat ccttggtggc tttatgatca tcggatcgtt tagccactta atcctctgca
      781 atacaccgag tcaaagttta acgtatctat tttgataagc ccctctgacg gcgagtccac
      841 aatcttgaca tttgcaatat ccatagttga tggaattgag tggagttcag aaaactgtcc
      901 attgtaaccg ttgaaagaga ggccgcaaca atcaagagca agcaaactgg aattgctctc
      961 atctcagtca cctattgtct tcagaagatt tttggcaact tttctgagtt cgacaatgat
     1021 ctcctgcggc ccaataacct tgaaggagtt ctcagaagcc aggaacatca ccaaatctcc
     1081 tatctcgaag aaatggtatt tatggcgatc gccaccaaaa tccaggtcta cttcgtggaa
     1141 ttcagcttct cgcgggaata ttcggttcca gagcattggt tcttctaaat atctcttgtt
     1201 cgaaaagatg accttatcaa tctcgtctga cctgacaaat cggacgttac catcctcttg
     1261 aagaccacaa aataactcac gtctttcatc aaaacaggcg ggatagaaaa tgaactcttt
     1321 gcctcctctt tgaaaaatct tcacagcttt cttactagct atcgcgttca gaaagactga
     1381 gcttattcga tattctgttc tcagttgaaa tttcatgctg tcttcgaaga gagaaggcaa
     1441 atgatagggt tcggaataga agtactcgcc tcttttccta gagtatttca tgggcgctcc
     1501 aagatttgct cttagatact tgaaatctct atgggcctgt cggactgaga tgccaaactt
     1561 ctgagtgtag tctttcatgg ccgcaaaatc attctttagc aagcgattgt gcagccacat
     1621 aatcctcatt tccctagaca catctgccac ctccagtggc ccagacgaga gacatctgaa
     1681 catcgcagaa cagaattcta aggtgctaga attgcaatag tattatacaa aaattcgaaa
     1741 agtaaatcta gaagatgaaa tggagacatg atggttagag gaatcatatt tgacttgttt
     1801 ggaaccattg tcagtaatgc gaggcttttc agacctgtat gctccaagat ggcacaagag
     1861 tcgtccgctg aagtggtgga aatagagaga gactttgtga atctctacgg tcactacttc
     1921 aaggactgtc ataaaatgcc ctttcaaccg gagaggtact attactatct tctgatcagc
     1981 gatttgattg acaagtacga tttgccagga gacatggaag cctactgtaa cttcatgtac
     2041 gattccttct ctaagttccg cgcctacccg gatgcaagga tcttaagggc gatcgtaaac
     2101 aagtattccg tcgcaatctt aactaatgcc gataattcct ttgttgagaa ggttgtcaag
     2161 agaaatagac ttccacatca ttttctgctt acctcggaat cggcaaggtc atataaacca
     2221 tccgaagtta tctttgagag gctcctgtct atgatggaaa tcgaaaagag gcacgctgtt
     2281 tttgttggcg acagtattca ggcagacatg cttggcgctt ctgcggcagg aatcaaagga
     2341 atccttatcg atcggtcaag gtcttactta gactacgcgc caagaatcga aagcttggaa
     2401 gaactgccta gcttgctaga gtccttgtaa gagcagcttc gaattcctca agaactatat
     2461 cgagatcttc tctggtaacg tttagattgg ttacgaatcg ccattcgcca gcttcaggcg
     2521 gatttatctt tattccctta tcgtgaagag tttcaacgat aaactttgaa cttgctttac
     2581 cggtgattct gaagaaaacc atgtttatgt cgagacgatc aaagaagact tcgatgtttg
     2641 gtaattttga aaggccttca gccaggtatt tagcattgtc gtggtctatt tgaagacgat
     2701 cgatcattct ctcgagtgcg aatactccgg cggcagcaat aatacctgcc tgacgcatag
     2761 ctcctcccat gatctttctt ccctttcgtg ccttggctat gaattctcgt ttacctacca
     2821 gcattgaccc tatcggtgca ccgagtccct tcgagaggca aaacataacc gagtcggaac
     2881 aagaggcaat ttctgcgggg ctaacccctt cggcaatagc agcgttgaat attcttgcac
     2941 catcaagatg cagggggact ttctgcgctg aagccagttc gtagatctcc ctgatgtagt
     3001 tcatggggag gattcgtccg ctggagtggg cattttccag acagattaat ccggttctcg
     3061 gataatgaag atcctcagat ctgatggcac ttctgattct ttcggctgaa ggtttgccat
     3121 catcacagtc aagagttctc atctgaactc ccgctattac tgcagaggca ccggcctcgt
     3181 ggacaatgat gtgattggac gctggaataa tcacctcatc tccccttaaa gtgtgtgtga
     3241 ggattgaaag ttgattcccg aatgttcctg aaggtacgaa aagggcggct tccttcttca
     3301 agatgtctgc tgacatctct tcaagtctgt tgactgtcgg atcgtctccg tacacatcgt
     3361 ctcctacctc tgcgctggcc ataacccgcc tcatttcttc accggggacc gtaactgtat
     3421 cacttctaat atctatccac ttcaagacag cacctccata aggtaagtta tgaaaacaat
     3481 tataaaccag aattactatg aagaataaaa aaagccggat ctaatgatcc ggactatatg
     3541 gggttcctat ctaaggggga taggggggtt ataaactata attattctag tttgtttgta
     3601 tgaatatcac tttgtgaaaa taaagcgaaa acatgcagtg tgcattacct atagattacc
     3661 tgttaaaaga aacattaaca ttaacacacc ggatgttaca tctcatgaat actatgggtt
     3721 gttacttatg gagtgagcgg taaccagatg gagtatatca atgttacact ttagaaaata
     3781 tcttatcagt aagaagagac agcaggacat ttgtggtgta agtggctgcg agggtaagtt
     3841 gcttgttatt gtgctttact ccgaaatacg gtaactttct tgtgtatgga gttcgatctt
     3901 cttctagcat ttggcaggga tctcaagaat ctttccagcg tttctttcgg atacttttcg
     3961 cattccccgc agtctgagat ttccttctct tcacagcaca gcataaattg gcacccactg
     4021 cagctgatgt tggtcgccaa aatcaaataa ccaaaatcct tcgaccaccc cagccattta
     4081 gagtctctct tcttagtgaa ttatcatcag caacagtgac cctgtatgta tcgcactgag
     4141 tgcagcctat cccgcagaat gctatcattg tcatgtacca ggtctccaat gaactaaggt
     4201 gtttgctcgt ctttagagca tagcattgtt gattgaactg ccatatcttt ggagggagag
     4261 ctttgaaacc tgtagttacc aaggaagttt acagagtcag atactacgaa ctggattgtc
     4321 aatggaaggc ttcaatttcc tcactaatgg attatttcaa tgacgtggtg actcttcaga
     4381 ctgtggagat gggtcacgga gttgacgtca tgagcaaggg cgaatacgct tggcttcttc
     4441 ttcgttggga tgtcaaagtg aacaggtatc ctgattattt ggaaaacgtg gttgttcaga
     4501 ctattcctta ttcaatggat agattttatg catataggcg tttcgagata tttgactgtt
     4561 cgggtaatgt cattgtcgac gcgaattctc aatggattct gattgaccag aggaagcgaa
     4621 gaccaataag gatcggagac cagttctacg aattatatgg tattgacagt gacttccatg
     4681 aacctctttc ttttccaaag gtcaacgaaa atgaatcttc tagtgaagag atcaccttta
     4741 tagtacgcaa ttcggacctg gatacgaacg ggcattctaa caacgtggca tatgttagat
     4801 ggattatgga gacagttccc agtgagtttg tgaagagatt cttgaagaga cttacaatcg
     4861 agtacaaacg cgaatctaga aagggcgatg taatttctat agagagtgta tttgagaacg
     4921 gagccgagtt tgctgaaggt aaacacaaaa taaccagttc tggaagagta ctttctcttg
     4981 ccagaactga atggaagtga aggactgctg aagccgccta ctaaacaagt gagtctggat
     5041 ttacataatc atccagccaa taagcatcgg gattcctgtc gaaagccata aagagatcgt
     5101 agcgttttgg atcggcagct ctatggtact tcatgtatat cttcttctcg tccaggccga
     5161 catattcgat tttgccggtt gcgtgcgaca ttacgaatct ggccctcttc gccaaacctg
     5221 agactgagtc tatggacttc ttgaaaatct cataaccttt ctcggccggc actgcataag
     5281 tgtgatttcc ctttgtgggt ctacactgaa agacatagta aggcggaatt ccaataaatg
     5341 agagtttcct gaatagtgct gtcattacgt cgacactgtc attcacgccg cgaatcattg
     5401 gcgtctgatt gcagattact gcaccggcat ctctcagaat gttcagacct cttatcgctt
     5461 catcggttag ctctctggga tgattgaaat gtgccattat atatatcctt ttctttgggg
     5521 tgctgtattt cttaaccatt tctggcaaat ctggatcgtt aataattctg taggggttaa
     5581 aagcaaccat tttgcttcca aatcttatta tgccaacatg gtcaatctcc ctcaactgtc
     5641 gaacaatatc tcccagtttt tctgtagata gtattagaga gtccccaccg gtgagaagta
     5701 cgttcgtgat ttccttgtgc tttcgaatgt actcaacacc aagagtaacg tcatttacca
     5761 cttctttgtt ttctgttgag aacagtctct tcctgaaaca gaatctgcag aagcttccac
     5821 aaacctttga gacaagaagc agggcggtat ctttgtactt atgttccata cctggagcaa
     5881 cggtgtattt atgctcctgt gaagcatcca gatcccccca ttcatcgagt tcttcgaagt
     5941 cgggaaggat tattctcttt atagggtcat gcggatcgtt ccagttaatg agccccagat
     6001 agtaatcatt ggccctgaag gggtaaacat cggtcacctt cttcatttca cttatctgtt
     6061 catcatttag ctcttcgatc ttctctacac tagtaagata tgttggtttc tgcaaatcta
     6121 tcactccttt aatatacaga ttaaaaatat acgaagcctg cattcgatca atccgaagaa
     6181 gcctttggca gagactgccc gcaatggtaa tcgacattga tcccttctag tgaatcttca
     6241 cttgtacgca aatcgatatt tgatctgaat caaacgagta agccggcatc gtgcaaatac
     6301 aggcaattgt gcaggcacta acaacaatta attatatcat actcaaaact ctgataaaaa
     6361 tgatgcagct gcactctctg agttgctttt caagtatgct tcacagaagt atactcacat
     6421 agggtatttg gtaaacagaa aagcccctct tcttggaggg gcggatagac aaaatctagc
     6481 gttacagtta ttcggtcact agtatgatat tccccacata atctccggaa ggagagtaga
     6541 tttttctgtt cagtgaatcg tctgtagaat cgaatatcgc cgttccgatt aatgtttctt
     6601 cagattcatt gatcgaatag aactgaatct catcgaaatt gaattcccag tcggtcttga
     6661 taacagcctg ttttcggtcg gaaacgattt catactcacc ggcaacaatc ctaacgtttt
     6721 cagaactaaa taaaggaagc gcatatgata tccttacaac cttttctgac gcttttactt
     6781 caagtctaaa tccctttttc gtcttcatac caagttcatc tgagacttct agaattacct
     6841 ctctttctcc gatgtcggct ctttccggaa ggaaaaagta tctatctccc acgacttcac
     6901 ccggaccaga aatcaggttg aatctgaggg cgtcgccatc tggatcagtt gcgaatgccc
     6961 gaaggtacaa agagaactcc tttccagcct ctgcgatgca atctggcaaa aaaacgtccg
     7021 gtggcctgtt cagatttctt acttcgattc tgattggaag actctggctt gagccattag
     7081 agtctatggc tgtgactgtg gctgtctttt caccgctgtc attaaacccg ggagtgtaaa
     7141 tgtaactatt ggctgatatg ctcccgatcc cctgtaattc gaattgaaga tcatctccgt
     7201 cagggtcaac gaaaagactt cccagatcga ggtcaagagt gaagctttcc atgatactgg
     7261 tgtttatcga aggtgataac gttacgggcg atctgtttac gttttctacc gtgactttga
     7321 agaaccctac gctttcaagt cctttgttat cttttgccgt tagtgtcact tcatgtattc
     7381 cttcggaatc gaagtcagga gtgaatagat aatggtcatc ctcaatgaag cccggtccgt
     7441 ctgcgatttc gaaggtgacc tcatctccat cgggatcgga aatgaaaccc ttcaggtata
     7501 tttttaactg ctctccctct tttaccgatt gatctggaat agatatttga ggaggtctgt
     7561 tgacatctat tacagatatg attattggta gagaaagact tcctccgttg ttatcagaag
     7621 ccgtaacaat aacgtttttc tcccccgcct cgtcatagcc cggtgagtac gagtagttac
     7681 tgccctcaat ctctccgata ccactgagac tgtatatcaa ttcgtctccg tctgggtctg
     7741 agaacagggg tcgaagatcg attttcaagg aagagccttc atgtatcgag gttttcatct
     7801 cttctattga cgattctggg tttctattcc tgtctttgac atcgattctg atcaccaagc
     7861 tatctgacag tccgtaagga tctgtggcag ttacagttgc aaccttttct cccttgtcgt
     7921 tgtaccctgg agagtactga tagctattgt cgacgactat accatagcct tccacttcga
     7981 attgaagatc atctccgtca gggtcaacga aaagacttcc cagatcgagg tcaagagtga
     8041 agctttccat gatactggtg tttatcgaag gtgataacgt tacgggcgat ctgtttacgt
     8101 tttctaccgt gactttgaag aaccctacgc tttcaagtcc tttgttatct tttgccgtta
     8161 gtgtcacttc atgtattcct tcggaatcga agtcaggagt gaatagataa tggtcatcct
     8221 caatgaagcc cggtccgtct gcgatttcga aggtgacctc atctccatcg ggatcggaaa
     8281 tgaaaccctt caggtatatt tttaactgct ctccctcttt taccgattga tctggaatag
     8341 atatttgagg aggtctgttg acatcattga ctctaaccat aaaagaagcc gtgtttgttg
     8401 ctcctttttc atcttcgaga attaatacga ccgatttgtc ccctgcatct gagtaatctg
     8461 gattgtatat gtaagatccg tcaacgatct ctccaggtcc ctctaggctg aaggtcacgt
     8521 tatctccatc tggatcgaca accctggaaa gcaaatcgaa agaaagggac tctccttcat
     8581 tcacgtttat gtcgctaagc aaggattttg gcgggcggtt cacgtctatt acgaggattt
     8641 cgaagcttga aaagacatcg ttacccgaac cgtctgatgc cttgattcta acggtttcag
     8701 ttccggaatc cgagtatgat ggctcgtaag tgaacactcc ttccgtaatc tctcccgggc
     8761 cttcttctag agaaaggacc actttgtcgt tatcttcatc gactaccttt gaagaaatat
     8821 caaccgagag aacctctcct tcattaacga tctgattctc gagctgtaaa acaggcgggg
     8881 cgttgacatc gattacttca agttcgatat ctcgtgctgt ttcgtttcca tttgggtcct
     8941 tcgccagtac tctcaaaacc gtcttgccgg aatcactgta gcctggagag taaatgtaag
     9001 cacttccgtg aagctcccct gggccttgaa cggagaattc aagttcttca ccatctggat
     9061 caactgctag cgagttcaag tcgatagtca ctgtttcacc ttcgcgaact acgataggag
     9121 aagtgtctat ttccggtggg cggttcactc taactacatt tatcagcatt tcttgctgag
     9181 aactctttcc cttcttgtcg gttgccctta tacttacacg aacttctccc tcataggaga
     9241 agcctggttc gaatacatag gaaaaaccgg atattgtgcc gggaccttct accagttcaa
     9301 gaactacttc atcgattttt tcatctataa cgaacggctt cagatttgca gaaagcttct
     9361 cgccctgatt cactacatga actggaaact gcaatttcgg ttctttgttt aagagtgcgg
     9421 gaagtgcaat aatcagaacg attgccacgg caactgcggc aatgagtaga tatatcatcc
     9481 atctcttcat tatttctccc cctttctcac gattgccttc tttcaattta tctgcagata
     9541 ttgatccaaa agactacgag taaagtttat attaattaat ctcctttctt tgattgccag
     9601 ctttctcaat ttccacatta gtttctaatg agttatcctg ctcaaacaga acaacaactt
     9661 cgtcaaaccc cattccgagg aagagaccgt ggaagaagtt tcttgcgctt tctctagatg
     9721 tctcttcaac tcccatttct gaggcgttct gaagtgcctg cttttctatt ttgtcgctgt
     9781 aagactgaaa aatcttcaac atatctttgt ttccaaagat ccttgtcaaa acaccactct
     9841 ggttgattac cctaatatcg ggaatcttac tctgaggaat tgtaacttcg agtcttggag
     9901 aaggcaggat cagcttaatc cttcttgctt caccttcatg gatttcgatt gaatcctctt
     9961 tcaactttct taggtctaca taacccctta cctgaaaggg aacaatgaaa ataaatcgct
    10021 gaaatgttcc gggaacaagg ctttcccgct caggttcgaa aatcagaagc gttgtcatcc
    10081 tcgcagtact cagttccgag attctctcca ctttgtgaat tatcgattct gtcctgaagc
    10141 tggtctttcg cctggacatg agaataaacg ctgcaagcag aatcacaagt gcaatcagaa
    10201 caacattgag tatcaactac gagtcacctc caaacactga cgaccatcag aaggtattca
    10261 taaaagtgtc attccgcctc ccttggcagc cattacatta cttcaatcag ttacatctaa
    10321 agtaaatcga gagttttgca catacagcaa aaaaatccct caactctcat tagtgatttc
    10381 tgagaactat atagattttg aatgagcgca agcaccaaat tccattactg atcttcggaa
    10441 aagatcttcc gccattcgtt cgaatttcca tcgattccaa cacttatatc ctgttgtggc
    10501 caaatgacca cacagaagtt gaatgagcaa gatagatcaa ccttgtactt cggaaatccc
    10561 aaagctggta cttaagcttt ctctttccaa cagaaatagg catcttcgat tctctttcat
    10621 accaagttta tcagagaaaa aagaaaatcc atcctgtaaa cagatttgtc gtttaaatac
    10681 ataagtataa taactctcgt gacgattctt atgatgatat aattaaaaca atctatatcg
    10741 gaattaaaaa gggaggtatg atttggatgg aatatcgcaa agttggtaaa agtggccttc
    10801 ttgtcagtga gttaagtctt ggttcatggc ttacctttgg ggctcagcta gatatagaca
    10861 atgcaagaga ggcgatgaga gaggcgtaca gagctggaat aaacttcttc gacactgctg
    10921 aagcatatgc cagcggaatg gcagagtcga tgatgggcga aattctgaag gaattcaagc
    10981 gggaagaagt agttgtttca acgaagatct tttggggagg aaacaaacca aatcaaaagg
    11041 gactctcaag aaagcatcta cttgaaggaa cctggaattc tctgaaacga ctccagttga
    11101 attatgttga cattctgttt gctcacaggc cagatccgga ggttccaatg gaggaaatcg
    11161 tccttgggat ggattacatc gtaagaaatg gtctcgcttt ctactgggga acatctgaat
    11221 ggagttccaa agagctcgaa gaagcccata aagtagccga caggctgaat gcaattcacc
    11281 cggttgtcga gcagccgcag tataacatgt ttgtgaggga aagagtagag tcggagtatc
    11341 gaccgattta cgagaagtat gggttagggc ttaccgtctg gagtcccctg gcgagcgggc
    11401 ttctgacagg taaatacaac aaaggaatcc cggaagacag tagattggca aagtttcccg
    11461 gcttgaagaa gcatctggaa gagaacaagc tcttttcgag tgagaatcta aaaaaggtgg
    11521 aaagacttgc cgccgttgcg aagaaactcg acactgagct tgctcagctt gcacttgcct
    11581 ggattttgaa gaatgaaaac gtcagttcaa taatactcgg agtcagcaag gttcaacagc
    11641 tgagagataa cctaaaggct ttggacgtaa aggataaact taccgaagag atagtaaaag
    11701 aaatcaattc cattattgaa tgaccgtttt cgagtgttct tttggaatga aaagagggag
    11761 gagaagtcca agatgaaaag gctcttgctt tttttgtcgg ttattttgat tatttttctg
    11821 gggagttctt gtctatacgt gatttcgctg ccgatcccag ttggcgaagg attgatagaa
    11881 cccatcgaat ttgaactccc cgggagtata ttgccgttat ctggttttgc tttcggaact
    11941 gagacagtcc catcgctcga cgagataatc caagaggccg aaaaagaaat ccagttaaat
    12001 cttccagata acttcgtgat taccggtatg gagatcaaag ctagagtaga atgggttccg
    12061 gagaaccctg attcggaaaa ctctattgac attgacttct atgtcttcag agagggacat
    12121 tcagaagaag aatttctggc ttttttcaac gatcctgatt caataagcga tcacaagctt
    12181 ttcagtggtt taatttatcc cggtgaaaac actttcaggt tgcattctgg cgaatccgct
    12241 gcactagata tgatactaga aataatcaac ggtggaacag ccgaagattt agattggttt
    12301 gcgatttacg actatgaagc accttcggat tcaactatca ctattcatct aagtggaact
    12361 atttcggtta agagagttgc agaataagac gtgaagttag atgaaaaagg tctccctgac
    12421 gggagacctt tttttctaca gtcgaatcta aagtatcttc tcgctgatgg attttccctg
    12481 aaggaaaagt agtaagtaat ctcttccgcc cgctttagaa tcagtgccgg acagattaaa
    12541 ccctccaaat ggctggacgc caacaagcgc tccggtacac tttctgttga agtagagatt
    12601 tccaacgtga atctctgtct ttgccttttc aattctgtct cttctcttgc tgtagaaggc
    12661 tccagttaga ccatattcag taccgttggc gattgctatc gcatgttcga aatcgcgcgc
    12721 ctctattatg gcggttacag gcccaaatat ctcttcctgt gcaattgctg ctgttggtga
    12781 tacgtcggca aatacagtag gttctatgaa atatcccctg cgtttcaaac gattaccgcc
    12841 agttataagc ttgctttcct tctttcccag ttcaatgtaa cggagtatct ttgatacagc
    12901 actttcgttt ataaccggcc ccatgtaaat gttgggatcg gttgtatctc caatggttag
    12961 agcttccgtt ttctgcttca ccagcttaac catctcgtca tagacttcac gaacaacgat
    13021 gatccgtgat cctgcagagc atttctgccc ctgaaaacca aacgcactcg ctacagctcc
    13081 agtcgacgcg gcatcggtgt ctgccgtttc atctaccact acacagtcct tgccacccat
    13141 ctcgagtatc actctcttta tccatttctg gcctttttga tgtttcgcag ccaactcgtt
    13201 gattctcaat ccgacctctt tggagccagt gaatgatacg aacctggtca aagggtgttc
    13261 aacaagataa tcgccaattt cactacctga acctggtaca tagttgacca caccatctgg
    13321 aagtcctgct tcccttaaaa tgtctacgaa ctttgcggcg ataaccggag catcggatgc
    13381 tggcttaagc aaaacacagt ttccggatac tgcggccgca gttgtcatac cggccattat
    13441 tgcactgggg aaattccaag gtgggattac cacgccgaca ccaagaggaa tatatctgag
    13501 ctcattgtat tcgccgggaa tttgaacaac gggctgagat tgtccatatc taagcgcttc
    13561 acgagagtag tattctagaa aatcgatggc ttcggctaga tctgcgtcgg cttctatcca
    13621 gctcttgccc acctcaagaa tcatcgtagc atctaattcg tatcgtcgct ctctcattat
    13681 ttgcgcagct ttcagaaaag ggactactct ttccgaagca ggcattcggc tccagctctt
    13741 gaaagcgaaa taagcagcat caatcgcttt gtcaacgatc tctctagtag cttttgaaac
    13801 cgttcctact acttcatcag gatctgcagg atttttcgag actattttgg tttcagtcga
    13861 aagatatctt cctccaataa acaagtcgta gtttctttca tgagaacgaa ttctttcaaa
    13921 agctagcatc atctgcgatc ttacttctgg atctgaaggg tcgatatatg gttcattctt
    13981 gaatggtgga attatctgga aatccgtaac atcaaacata gtaattacct cctacactac
    14041 gtttctttct ttggtgatgc catgttcaaa tctctcaagg ctgagatctg aaagatcggc
    14101 gctccttggc tcgccgtata tgatccactc ggcaactgcc tctcctactg ctggggccag
    14161 cataaatcca tgtcctgaga atccgactgc atagaagaaa ttctctaaat ctttggatcg
    14221 gtcaattatg ggcctcgcgt cgggagacat attataaaga ccggcccagt gtctaagcac
    14281 tctaacgttc ctaagaaatg ggaaaatccc tggcattttc ttagccattt ccgaaatgaa
    14341 ggtccaggtc ggctcgatat tgtgaccagg aagttcgtcc ttgctaccct ggcccataat
    14401 gaaatttcca gactttgtct gtcttatgta gaaattgttc tcaaaactta tgacaagcgg
    14461 atctagcata tgttccagcg gttctgtaac aaatatctgg tgtttataag attctgttgg
    14521 cagatcaatg ccagccattg cccctacctc tcgggaatat ccaccggcag cgttaacaac
    14581 gatttcagct tcaattttcc ccctgtcggt tactacaccg gttattcttt cgttttgagt
    14641 taagattctc agcgcggttg tgtgagttga aaaatcaact cccatcgctt tggcggcatg
    14701 tgcgtaagca aaagttgtta agtgaggatt ggcatgacca tcacttggac agtaagcggc
    14761 agcctttata ccttttaaat ctatcagcgg aaatctcttt gcggtttctt ttgccgaaag
    14821 cattaagaca tctagtccct cttcccgttg catcttggta ttcttttcaa agagagaaac
    14881 ctcatcgtct gtgtatgcaa gaagcaaata tccaccctga aagtattcaa tatcaaatcc
    14941 tacttccttt tggaattcct tgaagtgttt gacgcttcta attgcgagtt tcacattcga
    15001 cttttcagac cactgttgcc ttattccacc cccacaccgt ccagttgaac ccgacgaaag
    15061 atactctttt tcaataacaa caacatcatt caagccgttc ttcgcaagat agaaggcagt
    15121 cgccgttccg acgattcccc ctcctattat cactactttg tatttactcc tcataaccat
    15181 caactctgcc ttcaccaagt gctttgaaac taaccggtat tgagggcggc ctgaagacac
    15241 ctggagaaag tctttcgaca ggtaccccgg tttcctctga cagaatcatg agggtgttta
    15301 gcctgcaagt tctcccaccg catggcccca taccaattct caggtatcgc ctcagctctt
    15361 caaagtctgt ataaccttct ttaattgcct ttcttacttg atcaaccgtt acttcttcac
    15421 accgacagac catagcccct gattctttgg agggcaccaa gatatgtcgg acatctgaag
    15481 catactcgcg gagaacgctt atatagacaa gatttgtttt gttcgccgat ttgaccactt
    15541 tttctacctt cgccttgcaa attgcctctc catttcttcc cagagctgtt acatattgtc
    15601 cctcagcagg aagtggaagg aattcgtaag gaattccaac agaacatgtg ccatccttgt
    15661 tctctctaaa catgaaaatg gcaagccccg gacaagtagt tgcacagatg ccacatccag
    15721 tacactttga ataatctatg atcggtctac tgttgatatt atcaccgatg cttatggcac
    15781 cagccgggca acttgtttca caaggattac agggaattgc ttcgaagcat tcgattaatg
    15841 gtcgcatctt acccgtatac ttggtgaaat cattctgatt accatttgtg aatgacactt
    15901 cctcaaactt gcttccaagt ttagagagac cggctcttgt ctttgaagaa gtaggaccgc
    15961 ttctgaattt cctgagagtg tccttaagat acttcaatct ctcttgaatg ctattcttgg
    16021 tgaggtcact caccactgct aaacctgcaa tttgcccttc aatcatggcc gtagttgctt
    16081 cttcaatccc agcagcatca cctgctataa atatgtcatc tatcgatgtt ctcatgtcct
    16141 cgtctctgag agggacataa cctccaagtt cttggacata cttcatttcg cagccggcct
    16201 gggctgctag ttctattgat ggtaaaagtc ccactgcgat gcagatcgtg tcaacgacaa
    16261 gttccctctc cgttcccgaa atcgggctcc atctttcatc aagtttggaa atgacagctc
    16321 ccctcacgga ctcctctcca attgctctca aaattgtatg ctgcagcaga attggaattc
    16381 ccactcttct caccttgtca gcgtgaacct ggtaacctcc tatgcgattt gaagcctcaa
    16441 gtatcgctct aacctctaca cctgcctgaa gcagctgata agagactatg agtccgatat
    16501 ttcccgatcc aatcataagc ac
//
LOCUS       DAOQYI010000035        15528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_4248_length_15528_cov_69.554837, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000035 DAOQYI010000000
VERSION     DAOQYI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4248_length_15528_cov_69.554837"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..973)
                     /locus_tag="PKL93_08510"
     CDS             complement(86..973)
                     /locus_tag="PKL93_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ71339.1"
                     /translation="MSSSVFLQHLVNGISLGFIFGLIAIGYTLVYGVVKLVNFAHGDV
                     FMMATFFVYYGFTLFMMPWWLAVLFGIVVTILLGVGIERVAYRPLRDAPRISSLCAAI
                     GMSFLLQNIATVVFGGRQKSFYVPPSLTRTFVVGGVRIQAITITTIVVSIVVLLLLTF
                     LVKRTKMGLAMRALSVDFDTAKLMGINVNRTIAFTFAIGSALAALSAILWALRYPQIW
                     PFMGVFPGWRAFTAAIIGGIGSIVGALVGGLMLGMVTIMLVAFFPEAAGYRDALIFVL
                     LVVILLVKPTGLFGEKTSR"
     gene            complement(1075..2202)
                     /locus_tag="PKL93_08515"
     CDS             complement(1075..2202)
                     /locus_tag="PKL93_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71340.1"
                     /translation="MKRILVLASVLLMGFILFAVEPIVIGVFEPMTGPYAAGGQLTME
                     GINLAAEQVTEVLGRPIELVLVDNKSEKVEASNAVSRLIQFNKASVIIGSYGSAVAIP
                     GSEVANAAGVPMIGCSPTNPLVTLGKPYAFRVCFIDPFQGSVMAKFAVNELGAKTAVI
                     IQDIASDYSVGLSHYFQNSFKALTGDNKSISGVISYQTGDQDFTAQLAYAQGKNPDVI
                     FIPAASYGEAALIIKQARELGIESQFLGGDTWEVPEFLQVGGAAVEGSYFSTHFDVSV
                     APTPKAAEFIEAFKAKYGVEPSAFSALGYDAFMLAVDAIKRAGSAVPEDIKNALSETV
                     AFEGVTGYITIDENGDAIKDAIVRKVENGVFKFVSVVKPAE"
     gene            2920..3966
                     /gene="tdh"
                     /locus_tag="PKL93_08520"
     CDS             2920..3966
                     /gene="tdh"
                     /locus_tag="PKL93_08520"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNQ71341.1"
                     /translation="MESETMIAIVKERPAPGLTLKRVPVPRELGPHDVLVKVKRASIC
                     GTDVHIYNWDAWSQERIRPPQIDGHEFTGEVVSIGGDVSTVKVGDSVVSETHIPCQKC
                     LQCRTGKMHICKNMQILGVHRDGVFAEYVRVPEVVLWKVDPSIPLEYASIMEPFGNAI
                     HTALVTDLTGKNVLITGAGPIGVMAVAVAKVSGAAQVIVSEIKEFRKELARKMGADVI
                     IDPTKEDLTKKVKAMTEDNGADVLLEMSGNTTAFKQGLDSLTNGAVVSLLGVFPGELS
                     FDINGLFTFKGLTMYGITGRKMFETWQVATQLLKNKRIDLSPVVTHILPAEKFEEGFD
                     VMIRGISGKVILEF"
     gene            3990..5174
                     /locus_tag="PKL93_08525"
     CDS             3990..5174
                     /locus_tag="PKL93_08525"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNQ71342.1"
                     /translation="MFDFDVLKKEMEELEEKGLLVKIRTLESAQGAWLNIEGKKVLNM
                     CSNNYLGLCFDEELKEAAIDGIRKWGVGPGAVRSIAGTLELHNELETELARFKKVEST
                     LVVQSGFNANQSVIPPIVSEEDAILSDELNHASIIDGVRLTKAMRYVWKHKDVDDLEA
                     QLQKAETDGARRKLVITDGVFSMDGDLAPLPQIVEKTNKYGAILMVDDAHGEGVLGSH
                     GRGIVDHYGLHGSVDIEVGTLSKAFGIVGGFVAGKRVLIDYLKQKARPFLFSSSLSPA
                     ETAAAIAAVRKLSSSDELVKTLWSNSDYFKEQLKALGFDTGHSETPITPVMLYDAKLS
                     AAFSKRLFEEGIFASSIGFPTVPKGKARIRVMISASHSRKDLDFAVSKFELIGKELGV
                     IK"
     gene            5246..6211
                     /locus_tag="PKL93_08530"
     CDS             5246..6211
                     /locus_tag="PKL93_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNQ71343.1"
                     /translation="MKRLLVSLLMLAAGFALLIKGADYLIEGSVAIAKNLGISELLIG
                     LTIVAIGTSAPELAVSVQAAFKGSDIALGNVLGSNIANIGLILGITAILTPLGVNKTT
                     MSYEIPFVIIITIASSALILGNNGLHRSDGIVLITFFLIYMMYVFTMAKRDRSITDIV
                     EVEGKRDVEILEKSPVLAWVATIGGTAAVILGGNFVVDGGSSIAQIFGVSDMLIGTTI
                     VAIGTSLPELVTTISAGRKNRSDLAIGNVVGSNIFNLLLVLGISATISPITAQRSLAA
                     EIIFASLLAIVLPLLLLRKKKLDRPRGALLLIIYASFLVSSIFRG"
     gene            complement(6411..6623)
                     /locus_tag="PKL93_08535"
     CDS             complement(6411..6623)
                     /locus_tag="PKL93_08535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71344.1"
                     /translation="MGLVLAEREWNDSFRVWDILVWEKYQRRGLGSKLMGTARNYTTN
                     AIVRRLVVETQSSNYPAISLSLNYGF"
     gene            complement(6940..7683)
                     /locus_tag="PKL93_08540"
     CDS             complement(6940..7683)
                     /locus_tag="PKL93_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492612.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNQ71345.1"
                     /translation="MKEKTVIQKVGAAIMFLLAICFPASFVSASENCGPVALFLNDPA
                     YETKLSPIERSLAEQGIEYRIYNIFNDELPQDENLYSAVIIAGGDSMRNYIDWNNRIY
                     QGGEIILRGEVPILGICLGHQIISRVFGSVLYYSEERRWHEITFVKQDEIFDGFEDGL
                     LVWENHSYAVASLPEEFEILAKGNVTPLQMMKHKDKPIYGVQFHPETQGRFLSNPPGW
                     KLIMNFAKIAGVTMSDPPLPKDGRKVFPY"
     gene            7733..7820
                     /locus_tag="PKL93_08545"
     tRNA            7733..7820
                     /locus_tag="PKL93_08545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7767..7769,aa:Leu,seq:cag)
     gene            complement(7946..9430)
                     /gene="hutH"
                     /locus_tag="PKL93_08550"
     CDS             complement(7946..9430)
                     /gene="hutH"
                     /locus_tag="PKL93_08550"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNQ71346.1"
                     /translation="MLIDGQHLTLSDIFEVAFKKEFCSIEESAARLLDERRRSLELIS
                     KEKTIYGVNTGFGVLADHRISSKDLDALQKNIVLSHAAGVGEPLRQELVRAIMLVRAN
                     SLLKGFSGVRKCLVQRILDLLNEDIVPVVPSKGSVGASGDLAPLAHVAMSLIGEGYCT
                     LEGSVVPSLQALKSRNLEPIILKSKEGLSLLNGTAFMAGIGGCSTYIASRLFEQAILV
                     SAMSVDALMGSTSPFDERVHSARPHRGQGYVAGRMKSLLEGSEIRTSHLDCDRVQDAY
                     TLRTIPQVYGAVKDTLDYVKSVLEIEINSATDNPLIFENGDVISGGNFHGEPIALAMD
                     FLSIALTDMGNMIERRVDRLVNPKLNDLPAFLTNGDEGLNSGYMLWQYTAAALASENK
                     TLAHPASADSIPTSGFQEDHVSMGAWGARKLWMIVDNLATMISIEALLAFRALSFRHP
                     KKSSIAVEELFEDIRRIVPDHINDRYFGNEFMEVRKLLFRRAGL"
     gene            complement(9496..10167)
                     /locus_tag="PKL93_08555"
     CDS             complement(9496..10167)
                     /locus_tag="PKL93_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNQ71347.1"
                     /translation="MDRIWKIVVNYWPKFLEGLGVTLEMTLIAVVIGFIIGVILAIAR
                     VYGNKLFHGVATAIIEIIRGTPLLVQLFILYYGLPPLGMNLSPFAAAIIGFAINSGCY
                     QAEYLRGSIQSISGNQMKAARSLGMTKWQAILQIIMPQAFRRVIPAWTNEFIYLLKYT
                     SLAYIVGAPELMAQAKFIASRNFQFFEVYLVVAVIYLVVVLFFTKIFSILEKKVEIPG
                     LEWVR"
     gene            complement(10169..10924)
                     /locus_tag="PKL93_08560"
     CDS             complement(10169..10924)
                     /locus_tag="PKL93_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNQ71348.1"
                     /translation="MSKERVVLRVENLKKAFGDKEVLKGVSFDMKEGETKVIIGPSGT
                     GKSTLLSCINMLVTPDSGQIWLEEEEVTSAKNINKIRQEIGFVFQDFGLFNHLTALKN
                     VMVGLTKVKKIEKDFAKEIAMKELTRVGLEREANMYPAQLSGGQKQRVGIARALAMSP
                     KIILFDEPTSALDPELIGEVLSVMKNLAESGMTMLVVTHEMGFARTVSDEIIFMEHGH
                     IVEQSSPEEMFKNPKNPRTKEFLFKLSELYGSE"
     gene            complement(10921..>11513)
                     /locus_tag="PKL93_08565"
     CDS             complement(10921..>11513)
                     /locus_tag="PKL93_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNQ71349.1"
                     /translation="GLGLVLALPISFGQVYGNKWIKALIAVYEKILRSIPELVILFLI
                     FYGFPKIGIRFSPFAAVIIGLGFRSSAYQSQIFRGAINSVSSAQMRAARSLGMTNLRG
                     FINIVLPQAIRIALPPWTNEFTIVLKDSSLAYALGVTELLRQGGYIIATKYEPMLIYL
                     TVAVMYFIVTIAINKTLGSVEKRLAIPGFDVKETVR"
     assembly_gap    11514..11613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11614..12188)
                     /locus_tag="PKL93_08570"
     CDS             complement(<11614..12188)
                     /locus_tag="PKL93_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ71350.1"
                     /translation="MKKALLVLLALLVSTGFGFGAKYVVGTSADFPPFEYVENGEFVG
                     FDIDLIKAIAEEMDFEIEIRDMSFDSLIAALASGNIDIVISGMTITPEREEVVSFSKP
                     YWTADQSVVVRDDSELTVTVLFGKHNIGVQTGTTGDLWVEDNLVKTKILTGDFKRYDT
                     YVLAMTDLVNKNIDAIVLDAPVAESFAKVRPV"
     gene            12462..13535
                     /gene="mnmA"
                     /locus_tag="PKL93_08575"
     CDS             12462..13535
                     /gene="mnmA"
                     /locus_tag="PKL93_08575"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNQ71351.1"
                     /translation="MVMVMEMKIGIALSGGVDSAVAAARLIRQGNDVIGYHMIVLEGN
                     PLANAAADDAKAVADHLGIELKIVDLREEFRRILNYFTTSYMSGETPNPCVVCNDSIK
                     FGLLLERMFSFGEEKIATGHYARLVSLDENVFLARALDRAKDQSYFLSRIGKHKLHDI
                     LFPLGEMTKEEVRQEASGIDLPVHSKKDSQEICFIPDGDYRSFLKSRGIEGNPGPITD
                     ESGILLGRHTGLSGYTIGQRKGIGFSGRDRYYVKRLDLKNNKLILAQRQSLMSEALVA
                     KDPNWYIDPGEEFDCLCKIRSSMKPVSAKVTIVSGEKIRVDFQEKVWAVTPGQLAVFY
                     IDDLVVGSAFIEENCILESEGNT"
     gene            13550..13625
                     /locus_tag="PKL93_08580"
     tRNA            13550..13625
                     /locus_tag="PKL93_08580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13583..13585,aa:Pro,seq:cgg)
     gene            13778..>15528
                     /locus_tag="PKL93_08585"
     CDS             13778..>15528
                     /locus_tag="PKL93_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ71352.1"
                     /translation="MKRIGFLFLLFSLFLITTVYSKTLELKSWQIWDFSYNGIHGVCC
                     AVSVKNGEIPVTGLSFEDFTITETLVDEYGNVLSKAEIAPEQREDQFGGIGFWERSVS
                     SSKIDLVFLIDKTGSMADQIESIKSELKEFVNRLEEDASDFRVAVLLFEAALLEEPGR
                     KPPDHPFYGAMEMDELLLAIEEIETAGEWHINTWSYDAILFSSQLEFREDSRAVLVVI
                     TDTLPETVHGPFWYFSGGSVATKRAVEIALEERGIELEYFQPEESELAHMENYNRLIN
                     PALSESNFDHLVGAVSLGWPFKQDLIKVDALPVSESIYYLSWNSGLDEITDYYRKEGF
                     KVEVDVKCGDEHVSLSYSPFFDEEGYLQSSEDYHLPVLDEEGNQLPDKAVEMGLLREL
                     GDLKAFSGNYATKDGVLQLRSVDPGDYLYRLYAYGRSSYSYSTLRLKGTGKISFVQGE
                     VIPDFVVARTGDAFLESVKIRGLMDEFVSNKIAVNESKEFAEKSLKWIDAVASDGLNL
                     REMEALKRVYVSVGAFLNGMAYAAIESERVESDLLQMVRDTRSMTRKAGHRLQGMGSR
                     EENRQSIQVSMQLRKLSR"
BASE COUNT         4272 a         3419 c         3525 g         4212 t
ORIGIN      
        1 taattctcgg ctagccagag caacaagatg aaaacaagaa gtccaataat ggttattacc
       61 aacttcagtt tgcgatccat tacactcacc tacttgtctt ctcgccgaat agacctgttg
      121 gctttactag aagaatcacg actagaagaa cgaagattaa tgcgtctcta taacccgcgg
      181 cctcagggaa aaaagcgacc agcatgatcg taaccatacc aagcattaat ccaccgacca
      241 gagctcccac aatacttcca attcctccga ttatagccgc agtaaaggct ctccagccag
      301 gaaaaactcc cataaaaggc catatttgtg gatatctgag cgcccagaga attgcactta
      361 gcgctgcgag tgctgagcct atagcaaatg tgaatgcaat agtcctgtta acgtttattc
      421 ccatcagttt tgccgtatcg aagtcgacag aaagggcgcg catcgccaat cccattttcg
      481 tgcgcttcac cagaaaggtg agcaaaagga gtacgactat tgaaaccaca atagtggtta
      541 ttgttatcgc ctgaattctg acccctccta cgacaaatgt cctggtcagt gatgggggta
      601 catagaatga tttctgcctt ccaccaaaaa caaccgtcgc tatattttgg agaaggaagg
      661 acatgccaat tgctgcacag agcgaagaaa ttcttggcgc atctctcaga ggtctatatg
      721 caacacgctc gattccaact ccaagtaata tagttactac gattccgaac aaaactgcta
      781 gccaccatgg catcataaag agagtgaatc cgtagtagac aaagaaagta gccatcataa
      841 atacgtcgcc atgagcaaaa ttcacaagtt tgactactcc gtagacgaga gtatagccta
      901 ttgcaatcag cccgaatatg aaacccaacg aaattccgtt aacaaggtgc tgcaaaaaaa
      961 cgctagagct catcctgcca ccaccttcat cttagcgatc gttcttaata aggacgcagg
     1021 cgttggcctg cgtccggcac ctaagattct gcggatagat gccctttgtc ttatttattc
     1081 ggcaggtttc acaacactta caaacttgaa aactccgttc tccacttttc tgacaatcgc
     1141 atccttaatg gcatctccgt tttcgtcaat tgttatgtat cctgtaaccc cttcgaaagc
     1201 tacggtttca ctcaaagcgt tcttaatgtc ctcaggaacg gctgaaccag cccttttaat
     1261 cgcgtctaca gccagcataa acgcatcgta ccccagtgcc gaaaatgcgc tcggttcgac
     1321 gccatatttt gccttgaatg cctctatgaa ttccgctgct ttcggagttg gggctaccga
     1381 cacatcgaag tgagtgctga agtagctgcc ttcaacggcg gcaccaccga cctgcaaaaa
     1441 ctcaggaact tcccatgtat ctccgccaag gaactgagat tcaattccaa gttcgcgtgc
     1501 ttgcttaatg attaatgctg cctctccata cgaagcagca gggatgaata ttacatcggg
     1561 attctttccc tgagcatatg caagctgagc ggtgaagtct tggtcccccg tctgataaga
     1621 aatgacaccg cttatcgact tgttatcccc ggtaagtgct ttgaatgaat tttggaagta
     1681 atgtgaaaga cctacagaat agtctgaagc gatatcttga ataatcacgg ctgtctttgc
     1741 tccgagctca ttaacagcga atttagccat cacgcttccc tggaatgggt cgatgaagca
     1801 aactctaaaa gcatagggtt ttcccaatgt aaccagcggg ttggtaggcg agcatccgat
     1861 catcggtact cctgcagcat ttgcaacttc actgccaggt atggctacag cacttccata
     1921 gctgccgatt attaccgatg ctttgttgaa ttggatcaat ctggagacgg cgtttgaagc
     1981 ttccaccttt tcactcttgt tgtcgacgag aaccaactca atcggtcggc caagaacctc
     2041 tgtcacttgt tcagctgcga gattaatacc ttccatggta agctgaccac cggccgcgta
     2101 gggtccggtc attggttcaa agactccaat aacgatcggt tcaacagcaa acagtataaa
     2161 tcccattaac agaacagaag ctagaaccag aatccttttc acttccctca cctccacaat
     2221 tgtgattatt cacaaccgaa gtgacctcag aatgagcaaa aatactcgga gcacagcttt
     2281 gactttaccg aaagcaccga gacaactgtg cagaatgaga acatcaaacg ggtgaaaatc
     2341 ataactgcag cactggactt catgaacaac tgagacccaa taaccgctca ggaacgcgaa
     2401 aagtcttgtg gtactcatta agattgaaag acaacaaaac aacgcattac tcaaaatgat
     2461 aataccatgt gaaacaacaa caacctcttc tcaaaaaacg ccttcatgag gagattagag
     2521 caaagaaagc tgataatcgc tttctatcta tctggagacc aaagacgggc ggtcaaagat
     2581 cactaacgtt tttgctattg atcgagtaag atggagagtt gagagagtgc gtttagtaac
     2641 ttcttttgca gattgttgac atatgatgtc aaggggattc gacaggagtt tgtttgaaaa
     2701 ctcatctgtt ttcaaaatcg tccgtgactc gtcagatcat ttcaatggga agtgtttctt
     2761 gtaggaccta gccgttcagt acgctataag aagtatcaat caattcaaga gccgccatca
     2821 acaacatttg tccataaaca ggtggctgtc tgttttatga aacaatacga tcaacttaca
     2881 attacaatgt ggagtcataa atgaaaggag ggaatttgat tggaatcaga aactatgatt
     2941 gcaatagtta aggaaagacc cgcgccggga ttgactctaa aaagagttcc tgttccaagg
     3001 gagctcggtc ctcatgatgt actggtgaaa gtgaagagag cgtcgatatg tggcacagat
     3061 gttcacatct ataactggga tgcgtggtca caggagagga ttcgtccccc tcagattgat
     3121 ggccacgaat tcactggaga ggttgtaagc atcggtggag atgtttccac cgttaaagtg
     3181 ggagattcag ttgtatccga aacacatata ccgtgccaga agtgtcttca gtgtagaacc
     3241 ggtaagatgc acatttgtaa gaatatgcaa attctcggag ttcacagaga cggagtgttt
     3301 gcagagtatg tgagagttcc cgaagttgtc ttgtggaagg ttgacccttc tatacctctg
     3361 gaatatgctt cgattatgga gccttttgga aatgcaattc atacggcact tgtaacggat
     3421 cttacaggaa agaatgtgtt gatcaccggt gctgggccga ttggtgtgat ggcagttgcg
     3481 gtagcaaagg tatctggcgc cgctcaggtg attgtgtctg agataaagga attcagaaag
     3541 gaactcgcaa ggaagatggg cgccgatgta ataatcgatc caactaaaga agatctaaca
     3601 aagaaggtca aggcaatgac agaagataat ggggccgatg ttctgttgga aatgtcggga
     3661 aacactactg cctttaagca aggtttggat agtcttacca atggagctgt cgtctctctt
     3721 ctaggggtct ttcccggtga actttcattc gacataaacg gtttgtttac cttcaaggga
     3781 ctgacaatgt acgggattac aggaagaaag atgttcgaga cctggcaagt ggccactcag
     3841 cttctaaaga acaagaggat tgatctttct ccagttgtta cgcacatcct tcctgcggag
     3901 aaattcgaag agggatttga tgtcatgatt agaggaattt ccggaaaagt cattctcgaa
     3961 ttttgatctg acatttcgga ggtgttttga tgtttgattt tgatgttctt aagaaggaaa
     4021 tggaggaact tgaagaaaag ggtttgctcg taaaaataag aacgcttgaa tcggctcagg
     4081 gggcatggtt gaacattgaa ggcaagaagg tgctaaacat gtgctctaat aactatctag
     4141 gtctttgctt tgatgaagag cttaaagaag ctgctataga cggaataaga aagtggggag
     4201 ttggaccggg tgcagttagg tctattgccg ggacacttga attacacaat gagcttgaga
     4261 ctgaacttgc cagattcaaa aaagttgaat caactctagt cgttcagtcg ggcttcaatg
     4321 caaaccagtc cgtcattcct ccgatcgttt ctgaagagga tgcgattctc tccgatgaac
     4381 tcaaccatgc aagcattatt gacggagtca ggctgacaaa agcgatgaga tatgtctgga
     4441 agcacaagga tgtggatgat cttgaggctc agcttcagaa ggctgaaacc gatggagcaa
     4501 ggagaaaact ggtgataacc gatggtgttt tctctatgga cggtgatctt gcgccgcttc
     4561 cacagattgt tgaaaagact aacaagtacg gagcaattct aatggttgac gatgcccacg
     4621 gcgaaggagt tcttggaagt catggaagag gaattgtcga tcattatggt ttgcacggaa
     4681 gtgtagacat tgaagttgga acactttcca aagccttcgg aatagttgga ggatttgtcg
     4741 ctgggaagag agttctcatt gactatctca agcaaaaggc ccgtccattt ctcttcagtt
     4801 catcactttc accggccgaa acggcagcgg cgatagccgc ggtaaggaag ctctcctctt
     4861 ccgatgaact ggttaagacc ctctggagca actccgatta ctttaaagag caactgaagg
     4921 cacttggctt tgatactggc cactcggaga ccccgattac tcctgtgatg ctctatgatg
     4981 caaagctttc tgcggctttt tcaaagagac tctttgaaga ggggattttc gcaagctcaa
     5041 ttggatttcc gaccgttccc aagggaaagg ccagaataag agttatgata agcgcttctc
     5101 actcgaggaa ggatctggac tttgcggtct ccaagttcga actgatcggt aaggaacttg
     5161 gcgttataaa gtaaaaacac ggctttaact tcaattgaat ataatcttgt tgttgcagcg
     5221 gcaatggccg ctgctttttg tggaggtgaa gcgcttgtta gtttcgttgc tgatgctggc
     5281 cgctggattt gctttgctaa taaagggtgc cgattatttg attgagggtt ccgtcgctat
     5341 agctaaaaac ctgggaatct cggaattgct cataggactt acgatagtag ctattgggac
     5401 ttcggcgcct gagctcgcag tgagtgtgca agcagctttc aaaggttctg acattgctct
     5461 gggaaacgtg ttgggatcaa atatcgcgaa cattggtctt atcctgggaa ttacagccat
     5521 tctaacgccc ctgggagtga acaaaactac tatgagttat gaaatcccct tcgtcataat
     5581 aatcactatc gccagcagcg ctttgattct aggaaacaat gggcttcatc gaagcgatgg
     5641 cattgtgttg attactttct tcttgatata catgatgtat gtttttacca tggcgaagag
     5701 agacaggagc attacagaca ttgtggaggt ggagggaaag agagatgtcg agattctcga
     5761 aaagagtcct gtgcttgcct gggtagccac aatcggaggg accgcagccg tcattctcgg
     5821 aggaaatttc gtggtcgacg gaggttcttc gattgcacag atattcggcg tgagcgacat
     5881 gcttataggg acaactattg tcgcgatcgg aacttctctt ccggagttag tgaccacaat
     5941 ttcggcagga agaaaaaatc gcagcgatct tgccataggc aacgtggtag ggtcgaacat
     6001 cttcaatcta ctcctggttc tagggatttc ggccaccatc tcgcctatca cggcacagag
     6061 atcgcttgcc gcggaaataa tctttgcatc gcttctcgcg atagttctgc ccttacttct
     6121 ccttagaaag aagaagttgg ataggccaag gggggctcta cttctgataa tctacgcctc
     6181 cttcttagtt tcaagcatat ttagaggtta aaaattctgg ctcaatagaa tcaaagcaat
     6241 tttctgtttg tgaactgatc taaagatgtc tttttgcaga ctggcggatg ttgagaacag
     6301 agactttctg aatgcctgcg ttcaacctag agaagatagt gaaactcgag acggaccttg
     6361 tgtctttcga taactgtgtt cgaataacag gccgtatcga gtcaagctag ttaaaatccg
     6421 taattaaggg acaaagagat tgcgggatag ttggagctct gtgtttcaac aacgagacgt
     6481 ctcactatag cattagttgt ataatttctt gctgtaccca ttagcttact tcctaatccc
     6541 cttctttggt acttctccca gactagaata tcccaaaccc gaaaagagtc attccattcc
     6601 ctttcagcaa ggaccagacc cattttattc tcgaatgctc cccatgctac aggtttctgc
     6661 tcgaccggtt ctaacaagat atattctgta tctttactta actgaacagg agattctttt
     6721 agaggtactt cgatgtatag caatcatctt tccgtatcat tctaagatcg cagaatttat
     6781 cgcatgtata ggagtatctg acttttctgt ccaaaagcga ttctttactg agctcgatta
     6841 tttccatctt cttcaccatg cgcaacaccg ggtgttctca ctgaaatagc agaacttgaa
     6901 gctcaggttt aatggatcta tcatcaagaa gtttactgat cagtaaggga agaccttgcg
     6961 cccgtctttg ggcaaaggcg gatcactcat cgtgacgcca gcaatcttcg cgaagttcat
     7021 tattagtttc cagccaggag ggttgctcag aaatcgcccc tgtgtttccg ggtgaaactg
     7081 aaccccataa attggtttgt ctttatgctt catcatttgt aacggggtaa catttccttt
     7141 tgcaaggatt tcgaattcct ctggtaaaga cgccaccgca taggaatggt tctcccaaac
     7201 aagaagtcca tcctcgaatc cgtcaaatat ctcgtcctgt tttacaaagg ttatttcatg
     7261 ccaacgtcgc tcctcactgt aatacaaaac ggaaccaaat actctcgaaa ttatctgatg
     7321 tccaagacat attcccagaa tggggacttc tcctctaagg attatctctc caccctggta
     7381 aattctgttg ttccagtcta tgtagtttcg catcgaatcg ccgcctgcaa ttattactgc
     7441 ggagtacaga ttttcatctt gcgggagttc atcattgaaa atattgtaaa tcctgtattc
     7501 tatgccctgt tcagccagtg aacgttcaat gggtgagagc tttgtttcgt acgccgggtc
     7561 attcaggaag agagcaacag gtccacagtt ttcagaagca gaaacaaaac ttgccgggaa
     7621 gcaaattgct agtagaaaca tgattgccgc gccaaccttc tgaattaccg ttttttcttt
     7681 cattgactct tgacacctca tacagcgaat gataaactac tatttgtgcg atgccggggt
     7741 ggtgaaattg gcagacacgc atgattcagg ttcatgtggg cataccgcct gtgcgggttc
     7801 aagtcccgcc cccggcacca tattttctca gagaggatgc aatatttcct ctttttcatt
     7861 cttatattga actcaaactt tttgccttct gtatttctta aagtctgtga ctactcttct
     7921 atcttatttt agtttttttg gattctcaca atcctgctct cctgaagaga agctttcgaa
     7981 cttccataaa ctcgttccca aaatacctgt catttatgtg atcgggaacg attcttctga
     8041 tatcttcaaa cagctcctca acagcaatac ttgatttttt tggatgcctg aacgacagcg
     8101 ctctaaaagc aagaagtgcc tcaatagaaa tcattgtcgc gagattgtca actatcatcc
     8161 agagcttgcg tgctccccat gcacccatac ttacatgatc ttcctgaaaa ccagaagtcg
     8221 gaatagagtc ggcggaagcg ggatgtgcaa gtgtcttgtt ttccgaggca agtgccgctg
     8281 cggtatactg ccacagcatg taaccggaat tcagtccctc gtcaccattt gtcaggaagg
     8341 cgggcagatc gttaagcttt ggatttacaa gtctgtctac tcgtctctct atcatgttac
     8401 ccatatcggt gagtgcaatc gacagaaagt ccattgcaag agctattggc tcaccatgga
     8461 agttacctcc ggatataaca tcgccattct caaagatgag cggattatca gtcgctgaat
     8521 tgatctctat ttccaggacc gatttgacat agtctaacgt atccttgacc gcgccgtaga
     8581 cctgcggaat ggttctgagc gtgtaagcgt cttgaacacg gtcgcagtcc aaatgggagg
     8641 ttctaatctc gcttccttct agcaaacttt tcattcttcc ggcgacgtag ccctgccctc
     8701 tatgtggtcg agcactatga accctttcgt cgaagggaga cgtactgccc ataagggcat
     8761 caaccgacat tgccgaaacg agaattgcct gttcaaaaag ccttgaagca atatatgttg
     8821 agcagccgcc tattcctgcc ataaaagcgg tgccgttaag gagacttagc ccctctttag
     8881 atttcaaaat aataggttca agatttctac tcttaagagc ttgaagagaa ggaacaacac
     8941 ttccttcgag agtacaatat ccttctccaa taagtgacat ggcaacatgt gccaatggtg
     9001 caagatctcc gctggctccg accgatcctt ttgaaggtac gacaggaaca atgtcttcat
     9061 tgagaaggtc gagaattctt tgtaccaagc attttctaac tcctgaaaag cctttcaaaa
     9121 gagaatttgc tctaaccaac attattgctc ttactagttc ttgacgaagg ggttctccca
     9181 caccggccgc atgagaaaga acgatattct tctggagagc atctagatct ttagaagaaa
     9241 ttcggtgatc tgcaagaacc ccgaatcccg tattaacccc atagatcgtt ttttccttgc
     9301 tgatgagctc tagggatctt cgtctttcgt ctagaagccg cgcagcgctt tcttcaatac
     9361 tgcagaattc cttcttgaag gccacttcaa agatatccga cagtgtaaga tgttgtccgt
     9421 caatcagcac tgacccacct ccagaatcgt ttgattgttt tcaaacagat ttcagtcatt
     9481 atcagcattt cttaattatc ttacccattc tagtccgggt atctcaacct tcttttcgag
     9541 tatgctgaat attttagtga agaagagaac cacaactaga tagatcacag caacgacgag
     9601 atacacttcg aaaaactgaa agtttcgtga ggcaataaac tttgcctgag ccattagttc
     9661 cggtgcgcca acaatataag caagagaagt atacttcaag aggtatatga actcatttgt
     9721 ccatgctgga attactcttc tgaacgcctg gggcataatg atctgcagaa tcgcctgcca
     9781 ttttgtcatt ccgagagatc tggccgcctt catctggtta cctgaaatgg actgaataga
     9841 tcctctcaaa tactccgcct gataacaacc gctgttgatg gcaaaaccaa tgattgcggc
     9901 cgcgaacggg gagagattca ttccgagagg tggaagccca taatacagaa tgaacaactg
     9961 tacgagaagt ggtgtacctc ttattatttc aataattgcg gtcgcaacac catggaagag
    10021 tttgtttcca taaactcttg ctattgcgag gatgactcca ataataaatc cgattaccac
    10081 tgcaatcaaa gtcatttcga gagtaacccc aaggccttcc agaaatttgg gccagtagtt
    10141 aacgacaatt ttccagatcc tatccacttc attcactccc gtaaagctca ctgagcttga
    10201 agaggaattc cttcgttcga ggattctttg gattcttgaa catttcttca ggggaactct
    10261 gttcaacaat atgaccgtgt tccataaaga tgatctcatc agatacagtt cttgcaaacc
    10321 ccatttcatg agtcacaaca agcattgtca tgccactttc agccaggttc ttcatcactg
    10381 acagtacttc tccaatcagt tctggatcta aagccgatgt gggttcatca aaaagaatga
    10441 tttttgggct cattgctaac gcacgagcga ttccgaccct ttgcttctga ccacctgata
    10501 gctgagcggg atacatattc gcttctctct ccagcccaac tcttgtaagc tctttcattg
    10561 caatctcttt tgcgaaatcc ttctcgattt tcttcacctt ggtgaggcct accatgacgt
    10621 tttttagagc cgtaagatgg ttgaaaagcc cgaaatcctg gaagacaaag cctatctcct
    10681 gacgaatctt gtttatattt ttagcagatg taacctcttc ctcttcgagc cagatctggc
    10741 cactgtcggg agtaacaagc atatttatac aggaaagaag cgtactcttg ccggttccac
    10801 taggaccgat aattacttta gtctctccct ccttcatgtc aaaggatacg cctttcaaga
    10861 cttctttgtc accgaaggcc ttcttgagat tctcgactct tagtacgact ctttccttac
    10921 tcatcttacc gtctccttta catcgaatcc aggaattgca agtcgttttt caacgcttcc
    10981 cagagtcttg ttgatagcga ttgttactat gaaatacatt actgcaaccg tgaggtagat
    11041 cagcataggc tcatatttgg tggcgattat gtatcctccc tggcggagaa gctcagttac
    11101 tccaagtgcg tacgcaagtg aagaatcctt cagtacgata gtgaattcgt ttgtccatgg
    11161 tggaagggct attcttattg cttgaggaag aacaatattg atgaagcctc ttagattagt
    11221 cattcccaga gatcttgcag ctctcatttg agccgaactg accgagttta ttgctcccct
    11281 gaaaatctga gactgatatg ccgaggatct gaaacctaac ccaattatca ccgcagcgaa
    11341 gggggagaat cttattccga tctttggaaa tccatagaat attagaaaaa gaattaccag
    11401 ttccggaata cttctgagga ttttttcata gactgcaatt agggccttga tccacttgtt
    11461 tccgtagacc tggccaaaag aaatggggag ggccagaacc aggcccaacc ccannnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaccggtc tgacctttgc aaaactctct
    11641 gctactggcg cgtcgagaac aatggcatcg atgttcttgt ttacaagatc tgtcatggca
    11701 agaacataag tgtcgtatct cttaaaatca ccggtaagaa tcttggtttt gactagattg
    11761 tcttcaaccc agagatcacc ggtcgtgccc gtctggacac cgatgttatg tttcccaaac
    11821 agaactgtca cagtcaattc gctgtcatcc cttacaacaa cactctgatc agctgtccag
    11881 tatggtttcg agaatgacac tacttcttct ctttctggtg taatcgtcat tcctgaaata
    11941 actatgtcga tatttccgga agctagagca gcgattagcg aatcaaaact catatctctg
    12001 atttcaattt cgaaatccat ttcttcagcg atagctttga tcagatcgat gtcgaatccc
    12061 acaaattcac cattctctac gtactcaaat ggtggaaagt cagcgcttgt tccaaccacg
    12121 tactttgctc caaatccgaa cccagtcgaa accagtagag ctagtaacac tagtaaagcc
    12181 tttttcatga tacccctcct tatgctcaaa gatgaagtga ttgtatatca acatgctttc
    12241 attagaacga actgtgcgtg cagctttcga aaagaatcaa gaaaggtttg gcaacaatga
    12301 gttacagtgt agccatgacc tctcttcttt gattcgaaaa atgaaatgaa aaagctgtcc
    12361 aagttgccag tccggtgttc aagatataca ccttcttgag ttatgactac tcatagacat
    12421 tatacccttt agaaacactt gagccaaaaa caactgttaa catggtgatg gtgatggaga
    12481 tgaaaatcgg tattgcattg agtggcggtg tcgacagtgc cgtagcggcc gctcgattga
    12541 ttagacaggg aaacgacgta attggttatc acatgatagt tcttgagggc aacccgctag
    12601 caaatgcggc tgccgatgat gcaaaagccg ttgccgacca tctcggtata gagctgaaga
    12661 ttgtagattt gcgcgaggag tttagaagga tactcaatta cttcaccact tcttacatga
    12721 gtggggagac gccaaatcct tgcgtcgtat gcaacgattc gataaaattc ggattgctac
    12781 tcgagcgtat gttctctttc ggagaggaga agatagccac aggccattac gcaagacttg
    12841 tctccctgga tgaaaatgtt tttttggcaa gagcacttga cagagcgaag gatcagtcgt
    12901 atttcctttc gagaatcggg aagcacaaat tgcacgacat tctctttcct ctgggtgaaa
    12961 tgacgaagga agaagtcagg caagaggctt cggggattga tcttcccgtc cactcgaaga
    13021 aagactctca ggagatctgt ttcattcccg acggagacta cagaagcttt ctgaagtcgc
    13081 gggggataga gggtaatccg ggacctataa cggatgaaag cggaattcta ttgggaagac
    13141 ataccggact ttcgggatac accatcgggc aacgtaaggg aataggtttt tctgggagag
    13201 atcgctacta tgtaaagagg cttgatttga agaacaacaa actcattctc gcccaaaggc
    13261 agagtttaat gtcagaagcc ttggttgcta aagatcctaa ctggtatata gaccctggag
    13321 aagaattcga ttgcctctgc aaaataagga gctccatgaa acctgtttct gccaaagtga
    13381 ccatcgtaag tggtgagaaa attagagtag attttcaaga gaaagtgtgg gcggttactc
    13441 ctggacaact tgccgtcttc tatatcgacg atctggtagt aggaagcgcg ttcattgagg
    13501 aaaactgtat tcttgaaagt gaagggaaca cgtgatagaa ttttgcagac aggaagtagt
    13561 tcagttggaa gaacgctggt ttcgggaacc agaggtccgg ggttcgagtc cccgcttcct
    13621 gaccagagca ggcccagtgg ggtctgtttt ttttggccca ggaaaatcac ctgactctcc
    13681 agatgatcgt ttgtttgagt tgatctgcta gaatttgctc acagagattt gttctctttg
    13741 tcttctcctc tgatcgatcg atctggaggt ggatcttatg aaaaggatag gcttcctatt
    13801 tttattattc tcactttttc tgatcacaac tgtttattcg aaaacgcttg aactgaaaag
    13861 ctggcaaata tgggatttca gctacaacgg cattcatggg gtttgctgcg ccgtcagcgt
    13921 caagaacgga gagatccccg tcacgggact ctcatttgaa gacttcacca taaccgagac
    13981 tctcgtagac gagtacggga atgtcttgtc aaaggccgaa attgctccgg agcagagaga
    14041 agaccagttt ggtggcattg gattctggga aagatcagta tcttcctcga aaatcgatct
    14101 ggtctttctc atagataaga ctggctcaat ggcggatcaa atcgagtcga tcaagtcaga
    14161 actgaaggaa ttcgtgaatc gactcgaaga ggatgcaagt gacttcaggg tggcggttct
    14221 gctatttgaa gcagcgttgc tggaagagcc cggaagaaaa ccgccggacc atccatttta
    14281 tggagctatg gaaatggacg aacttctcct cgctatcgag gaaatcgaaa cggcaggcga
    14341 atggcatata aacacctgga gttatgatgc aatcctcttc agcagtcagc ttgaattccg
    14401 tgaagactcc agagcagttt tagtagtcat tacagacact cttccggaga ccgttcacgg
    14461 accattctgg tacttttccg gaggcagtgt tgcaacgaaa agagccgtgg aaatcgcgct
    14521 ggaagaaagg ggcattgagc tggaatactt ccagcccgag gagtctgaac tggcgcacat
    14581 ggagaattat aacaggctca taaaccccgc attgagcgag tcgaattttg accatctggt
    14641 aggagccgtt tctctcggct ggccattcaa acaggatctc atcaaagtcg atgcgctgcc
    14701 cgtttctgaa tcgatttatt acctctcctg gaattctggt cttgatgaga ttacagatta
    14761 ctatcggaaa gaaggtttca aagttgaagt ggacgtaaaa tgtggtgatg agcatgtctc
    14821 tttatcgtat tcgccatttt tcgatgaaga aggctatctt cagagttcag aggactacca
    14881 ccttcccgtt ctcgatgaag agggtaatca gttgcccgac aaagccgtag aaatgggttt
    14941 gctgagagag ttgggtgacc tgaaagcatt cagcgggaac tatgctacga aagatggagt
    15001 tctgcagttg cggtcggttg atccggggga ttacctatac agactctatg catatggaag
    15061 aagttcttac agctattcca cacttcggct aaaaggtaca gggaaaattt cattcgttca
    15121 gggagaagtg ataccggatt ttgttgttgc ccgtacgggc gatgcatttc tcgagtcggt
    15181 taagatcaga ggcctcatgg atgaatttgt aagcaacaag atcgccgtga atgaatctaa
    15241 agagtttgca gaaaagtcgt tgaaatggat agatgcggtc gcttctgacg gactgaacct
    15301 aagggaaatg gaagccctta agagagttta cgtttcagtt ggggcgtttt taaatggaat
    15361 ggcttacgca gcaatagaat ccgaacgggt agagagtgat ctgctgcaga tggtgcgtga
    15421 tacgaggagc atgacgcgaa aggcagggca tcgattacag ggtatggggt ctcgtgaaga
    15481 gaaccggcaa tcaattcagg tttcaatgca actgaggaag ttatcccg
//
LOCUS       DAOQYI010000036        14782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_4517_length_14782_cov_72.107558, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000036 DAOQYI010000000
VERSION     DAOQYI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 14782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14782
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4517_length_14782_cov_72.107558"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..1416)
                     /locus_tag="PKL93_08590"
     CDS             complement(286..1416)
                     /locus_tag="PKL93_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ71353.1"
                     /translation="MNPGIIFFEALIYSSLATKNLLSQYFDISGFSPTKIGILMAVLP
                     AVSLVSSPLWFKLSGRFGYNKIYYIVSLSSALLIWPIFVVKSFTTTLFMMILLSFFFS
                     AVVPLGDSIIINKLKSSGGRFDRVRLFGTIGFAITSLALSRLVGLSFFWLFAASAAVL
                     VISSLFISPKTDDLETADQQHLSGTGSTFQLTMMTVGMFVGVTLNSFHNTFIAVYARE
                     NGMDSSVVGMVFAITALSEIPFLLFADRIIEKIGNMKILLFGMIVMGVRMILISYATN
                     EVLLYLVEFSHGLTYILMYYSLYHFIHYELSGKSLVKAESLFWVVRSGMTFIVGSIGG
                     GIVIDLLSVTFAFRFFGFVGVVSSVVMIIFYIVISRRKNALI"
     gene            1487..2413
                     /locus_tag="PKL93_08595"
     CDS             1487..2413
                     /locus_tag="PKL93_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNQ71354.1"
                     /translation="MKIITRIILITVFLSVSTAFSSTGNFDVQGHRGCRGLMPENTLA
                     AFNFAMKLGVDTIELDTVVTRDGVVVISHDPYLNPERTRKDGEFISESILIKNLTFEE
                     LKGFDVGSIREPDKWPEQKQLDGERIPSLEEVLSLIRVHNNSNAKQIKVNIEIKHYPQ
                     RPEDTIDLTEHVRKVLEIVCEKGFERLCTIQSFNWEALKISKELQPSVETAALVSSTN
                     LDKTVWTAGLRLKNFGFDIGKMLATIGCNIISPNYNLMSDGWVKSAHQSGMKIIPWTV
                     NERDEMERLIGLGVDGIITDYPDRLLEVVSNQ"
     gene            complement(2541..3464)
                     /locus_tag="PKL93_08600"
     CDS             complement(2541..3464)
                     /locus_tag="PKL93_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71355.1"
                     /translation="MRTLEVKDLRSRIGTFNLGPIDLFVEKGEIVGLIGPSGCGKTLL
                     LRTIAGLQEPLSGTVTINGEDVTFLEPHLRGLAFVFQENALFPHMDTHNNIAFPLLIS
                     RDEEADVKVAKKAEELDGLSNYLDRWPKELPAGMKKLTAFARETVKKFNIIMLDEPFE
                     RLDKKIRTELRSTMKRLLMALGESVLIVLNDPEDALAVADRVYVMDNGNVVQQGPPRE
                     VYDEPNSLFVMELFSTMGINRVGDNVFRPEDVILSEFGEEFIPEHCGPYDSKRIICNG
                     RIRGEYVTLLLPLECRDMDSITLEITKSFDL"
     gene            3690..4880
                     /locus_tag="PKL93_08605"
     CDS             3690..4880
                     /locus_tag="PKL93_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNQ71356.1"
                     /translation="MSRHLLSKAEEYREELTRFLSDLVSIQSFSAGEKEVVKRIEVEM
                     NKVGFDEVKIDGLGNVLGRIGNGKKIIAMDAHIDTVEVGNERLWKVDPFGGLVSDGII
                     YGRGASDQKAGMAAMVYGAKIMKEEELLGDFTLYITGTVMEEDCDGLCWRYIIKEDGI
                     RPDYVVITEPTNLNIYRGHRGRMELQIRTSGLSCHASAPERGINAIYRMSRIIQQIEV
                     LNERLKDDPFLGKGTIAVTQIFFKSPSQNAVADECTIQIDRRLTAGETKETVIEELKD
                     AIRISGEEAEILELFYERPSYTGLVYPVEKYFPTWVMDESSEIVQKTVNTYKEVFGEE
                     PFVDKWTFSTNGIATAGVFAIPTVGFGPANEIFAHSPDDQCPVDHLVKAAAMYALLPL
                     RLSQ"
     gene            4897..5886
                     /locus_tag="PKL93_08610"
     CDS             4897..5886
                     /locus_tag="PKL93_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNQ71357.1"
                     /translation="MSSILRGKDFITTQDFDRTEIDLMLDLSSDLKRRFATNDPTPLL
                     PYKTVFLMFFDQSTRTRNSMEAGITQLGGHAHYLDPSTMQTAHGEVPRDTAMILSRYG
                     HAIAVRHCKFKVGNSYLRSLAENADVPILNLQDDIYHPMQVMADIMTMRERFGANLSG
                     LKVAITWAYAESHLKPLSVPQSQILLFTRYGMDVKLAYPEGFDLMPDIVDLAKKNAEG
                     SGARLEILHDMDEAFRDADIVIPKSWGGFYVSDDPQEIMTEVRRNKDWICTPERMKLA
                     NKRAIYMHALPADRGREVVDEVIDGPQSVVIDEAENRLHTAKAIMALTMGGRS"
     gene            5883..7265
                     /locus_tag="PKL93_08615"
     CDS             5883..7265
                     /locus_tag="PKL93_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ71358.1"
                     /translation="MTYDLALENGTVCTENGLFPANVYVEGEEIRVISSIKEKLPAKE
                     SIDCSGLFLLPGFIDPHVHLGLALGRYTSADNFESGSALALSGGVTTLIDFLDPISDL
                     AQLEAAKRDRMTAAKDSRVDYSFHVTLAGNPSFSAEEIARSSVAAKMPSIKVFTTYSS
                     SGRRSSDGMIYSLLSLSRKLGFVLMAHSENDEIIREREKEFSRPTYRDISSLRPTISE
                     LSEVAMLSLISLEEEGQLYIVHVSSGKTVEALSSLGLDWRRFTKLETCPQYLLLNNDP
                     LKTDQGYLNTYCPPPRTDQERALLQSALRKGEISSIGTDHCPFLNREKWENRDDYPTI
                     PNGTGSLGISFATINTILDGDLKRVSSLLSTNPARIFGLFPKRGVLAPGSIANIAIVD
                     RDVQSTVSRSRYTRSDHSLFEGIKLRGSVETVIHRGKLAFKNGDILLPKASGRFIERE
                     NIYWEKGTCK"
     gene            7256..8554
                     /locus_tag="PKL93_08620"
     CDS             7256..8554
                     /locus_tag="PKL93_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ71359.1"
                     /translation="MQINNVSIYTNDGDYIPKGFVRVENGKIVKVAAGESAYNGENFY
                     FEGKLLMPSLVNAHTHIYSTFARGLKVAPFNTSSFTKLLEQLWWRLDRAMTVEDLKIS
                     AYVAAIESLKSGVTSLFDHNSSPNAIEGSLRGIASSVNSVGLRYCGAYEVSDRDGTER
                     RDKGIRENLDFSSESSAYRKGIFGLHASFTLSNETLSLASREIAGKIPIHVHVAEGPE
                     DQEYTVSVHDSRVLERFRRAGLMTENSIYAHCIHTTPSERTLVKDSRGYIVVNCQSNV
                     NNGVGFPEWRNFANEGIRILLGNDGYGFNLCHDVRFLILCPHYIYRDPKVSSSGDIGR
                     SLFGSNYDLATRTFGVNLGTIREGSSADFILLDYKSPTEITAENFVDHFFFGICENIA
                     VSDAFVGGERVLAEGRPTRVNEDDIYKEARKLYKAFEKRF"
     gene            8566..9645
                     /locus_tag="PKL93_08625"
     CDS             8566..9645
                     /locus_tag="PKL93_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ71360.1"
                     /translation="MKLATEIAGIHLENPLMPASGPLTGDQRKMRAIEAMGVGAMVTK
                     TISTVAAKVPRPCIIAERDFVINTELWSEFSPEKWLNEFLPDYRKKSALPLIVSLGYS
                     PEDLRKLVPRFAKFADAFELSTHYVADDPSLIRELISAVKAGTEKPVFLKFDPSVPDP
                     AEMARVVQESGGDGIVAINSLGPVYPMDRRVNRSYLGSRDGFGWISGPVIRRLSLSSV
                     RRIREGCTLPIIGVGGIVSADDVVDFLCAGASAVQMLSGALIRGKGIFKRILESLPSA
                     LEKRGFKSVEEAIGSAKDQKESFTVRNPVIDHDKCTRCGLCVEVCPYFALSLQEKVEV
                     DSAECFGCGLCESICPVAAIGGVLV"
     gene            9642..10898
                     /gene="thrC"
                     /locus_tag="PKL93_08630"
     CDS             9642..10898
                     /gene="thrC"
                     /locus_tag="PKL93_08630"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNQ71361.1"
                     /translation="MSKFLLKCITCGKEFSEEEEEYYCPVCGERRGTLEVVYDFDQVR
                     EKYESSSVDLNNRGIWAFDFLLPFENCTDTPRLFVGNTPLYRNHFLEEKCRVSELLLK
                     DDGRNPTASFKDRASAVAVLKAKEKSFDTVFCASTGNAASSLSGFAAVSGLKSVIFVP
                     ATAPAAKITQLQVYGSKILAINASYDRVFDLSMEIGMSKGWYCRNSAINPYLLEGKKT
                     CAMEIAFKTKDRLPDFVFVSAGDGTVLSSFYKGFEDLMKLGVISKIPTIVGVQAEGSN
                     AIMRAYERGYPFEPVDIEARTVADSISVGKPRDVFKACRYTQKHNGLFLSVSDGEILG
                     SIIELARETGVFAEPAGATAFAGFKKARRLSIVNERNSVVIVVTGNGLKDLKSVSYVL
                     PKVETLPPVKDAIEEALNEDQLHTQQ"
     gene            10870..11346
                     /locus_tag="PKL93_08635"
     CDS             10870..11346
                     /locus_tag="PKL93_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNQ71362.1"
                     /translation="MKISFTLNNERLSVDVAEDMRLLDFLRDELGLTGTKEGCGEGEC
                     GACTVIIDGKAVNSCLVLLPEIDGSEVTTIEGLSKDGELDTIQKAFVDEGAVQCGFCT
                     PGMIMSAKALLDRNDSPSDEEIMEAIEGNLCRCTGYYKIIQAIKIAAENLRKTNGK"
     gene            11336..11911
                     /locus_tag="PKL93_08640"
     CDS             11336..11911
                     /locus_tag="PKL93_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNQ71363.1"
                     /translation="MESNSVSLILLAAGESSRFGDEKLCSNFRGKPLLQWSLDNLKNV
                     GVLNKFLIIKPGFELNRFDTGSFKILINDDYKKGISTSIVRGLCECAEERTGIVLMLA
                     DMPLVSGEDVEFLLESLLPEDEMVSFVYDGRKGFPTFIAKKLFPELLKITGDRGAFQL
                     VKNGQAKIREIEGSERNVFDIDSPEDRLQIW"
     gene            11972..12811
                     /locus_tag="PKL93_08645"
     CDS             11972..12811
                     /locus_tag="PKL93_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNQ71364.1"
                     /translation="MSFNVLMPTSEEELVSLLSNESSHILAGGTDLIVKIRLKRVIPK
                     IVVSLKHLKGLDTIEDLGDKIRVGCRVTISELLKSEIIDRHGRIVQMALRQIGSPQIR
                     NRATLAGNIVNASPAGDSIVAMVLTNASLSIGSANGERYERVVDFIKGPGETTLSRGE
                     FVKSITFEKTDALFPQFYKVGQRNSMAISVASVGSLTSESEIRLAFGSVAPTVVVPIE
                     ACEYYSAKNGSFDEERFVELAMKCVTPIDDVRATAWYRTTVVRNLIHRTLKVWRKKGY
                     NAV"
     gene            12801..13637
                     /locus_tag="PKL93_08650"
     CDS             12801..13637
                     /locus_tag="PKL93_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNQ71365.1"
                     /translation="MLSNVNEYFRPKTVEEAYELMQNDRDHSIFLSGGTIVALMESSR
                     IEKIIDLKSLRLDTISVTEGEIVVGATTTIESFRKDPVIRKEFDDFFYNAFSLVGSWQ
                     IRNMATIGGSVAPKLGWSDVSTCLLTTGSSLMIYGVDGYQRMLVSDYFSLPTGEKPLI
                     THVMLKRDGWVSAFEKFSKTTFDIATVNVGLSIIPERNRIKGARVVCGSRPQYPARFE
                     SVEAAMTDLEINDVMPAIVRSLVYESFSGGSNMTASFEYRKHLASVLAQRAAEKVKEM
                     IT"
     gene            13634..14107
                     /locus_tag="PKL93_08655"
     CDS             13634..14107
                     /locus_tag="PKL93_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNQ71366.1"
                     /translation="MKIALTVNGKLHDIDIDPGEYLLDVLRRIGYKSVRRSCNSASCG
                     TCTVLLNGKPILSCSTFAASVDGQNIETVEGMADGEKLHPIQEAILEEGGAQCGYCIP
                     GLIMAGEALLRENSDPSDEEIKLALNGNICRCTGYEPQNRAIKKAAKVMRGELDD"
     gene            14100..>14782
                     /locus_tag="PKL93_08660"
     CDS             14100..>14782
                     /locus_tag="PKL93_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ71367.1"
                     /translation="MTDSFVTVGKPKRKVDGLAIVRGKPVYTPDYDMPDALVVKLLRS
                     PHAHALVKNIETEQAKLINDVVDIFTFSDVERIPYTRAGQGYPEPSPYDTFLLDRKVR
                     YVGDPVAIVAAKTEKAAKDALRNIKVEYEILDAVLEMKEATKEGAPIIHDESECAGVY
                     DKSRNIAAHYEMDLGDVEETLKTCDVVIERTYHIDTQLHAALEPHNALTYLDSVGRLI
                     VITSTQVPF"
BASE COUNT         4413 a         3098 c         3512 g         3759 t
ORIGIN      
        1 actaacacaa gaaacttttt cataataccc ctcccttttc aaaaattgaa aacggtggaa
       61 cgttcattca gattataata ccgtgctcag attccagtaa gctcgccatg agcttaccat
      121 gcccgattat tggcttctag aactggaaag acagttgttt ctgcccgatc ggaagaaata
      181 attgaatatc tgttttttgt gttgcagttt caatatcact attgagtagg agatttgaca
      241 gagctgacat ttttgatgag cttcaaagcg aatgttaaaa gaagctcaaa tcaacgcatt
      301 tttccgcctg ctgatcacta tgtagaaaat aatcattact acagacgaga caactccgac
      361 gaatccgaaa aacctgaaag caaaggtaac tgacaacaag tcaatgacga ttcctcctcc
      421 gatagatccc actatgaaag tcatccccga tctcacaacc cagaaaagac tttcggcctt
      481 cacaaggctc ttaccggaaa gctcgtagtg tatgaaatga tacagggagt agtacatgag
      541 aatatacgtc aagccgtgtg aaaactcgac aagatagagc aaaacttcgt ttgttgcata
      601 agatatgaga atcatcctca cccccattac gatcattccg aacagcagaa tcttcatgtt
      661 tccaatcttc tcgattattc tatcggcaaa tagaaggaag ggtatttctg aaagcgcggt
      721 aatcgcaaag accatcccga caacggacga atccatacca ttttccctgg catagacagc
      781 aatgaaggtg ttatgaaagg aattcaacgt aactccgacg aacatcccaa cagtcatcat
      841 tgtgagttga aaagttgagc ctgtacctga aaggtgctgt tgatctgccg tttccaagtc
      901 atccgttttt ggactaataa agagagaaga gataacaagc actgctgccg atgctgcaaa
      961 caaccagaaa aaactaagac ccactaatct gctcaaggcc agtgaggtaa tcgcaaaacc
     1021 aatggtcccg aaaagtctta cgcgatcgaa ccgaccacca gaactcttca acttattgat
     1081 gattatcgag tctccgagcg ggacaacagc agagaaaaag aatgatagca agatcatcat
     1141 aaataaagta gtcgtgaaac tcttgacaac gaatatcggc catattaaca atgctgacga
     1201 aagagataca atataatata tcttgttata accgaatcta ccgctgagtt taaaccataa
     1261 cggactcgag accagagaga cggccggcag caccgccata aggatgccga ttttcgttgg
     1321 agaaaaaccg gaaatatcaa agtattggct gagaagattt tttgtggcca aactcgagta
     1381 aatcaaggct tcaaagaaga taatacctgg gttcatgcga tctccttccg aattactagt
     1441 agattataca accaataatt taaaactgtt aacaggagtg tgatttatga agattattac
     1501 aagaattatt ttgattactg ttttcctttc cgtctccaca gcattttctt caaccggcaa
     1561 ttttgatgtt cagggtcatc gcggctgtag aggacttatg ccggagaaca cactggcagc
     1621 tttcaatttt gctatgaaac tgggcgtgga tacaatcgag ctagacactg tggtaacaag
     1681 agatggcgtc gtagttatta gtcacgaccc gtacctaaac cctgaacgta ctagaaaaga
     1741 cggggagttc atcagcgaat cgattctcat taaaaatctg acctttgagg agctaaaagg
     1801 attcgacgtt gggagtatca gagaacccga caaatggcct gaacaaaaac aactggatgg
     1861 agaaagaatt ccatcccttg aggaagtact ttctctgata agagtgcata acaacagtaa
     1921 cgcaaaacaa ataaaagtaa atattgaaat aaagcactac cctcagaggc ccgaagacac
     1981 aatagatcta acggaacatg ttcggaaagt tcttgagata gtttgcgaaa aagggttcga
     2041 aagactatgt acaatacagt ccttcaactg ggaagcgctg aaaatctcca aggagcttca
     2101 accttctgtg gagaccgccg cactagtatc aagcacaaac cttgacaaaa ctgtctggac
     2161 agcaggttta agattgaaga acttcggatt tgatatcgga aagatgcttg ccaccattgg
     2221 atgtaatatt atttctccca actataactt aatgagtgat ggttgggtga aaagcgctca
     2281 tcaatctgga atgaaaatca ttccctggac agtgaatgaa cgggatgaga tggagagatt
     2341 gatcggcctt ggtgttgacg gaatcataac cgattatcca gacaggcttt tggaagttgt
     2401 ttcaaaccaa tagaaaagcc tttctccttg agaaaggcct ctcataaaaa cgttttacac
     2461 atcaccaata ctgattatac aacatttcaa aggatattag ccctaactat agtgaacaaa
     2521 ggcaaagcaa cactctgcgt ttacagatca aatgatttcg taatttcaag tgtaatcgag
     2581 tccatatctc gacactcaag aggaagcaac aaggtcacat actcaccccg tattctgccg
     2641 ttacatataa tgcgctttga atcgtagggg ccacagtgtt ccggtatgaa ctcctcaccg
     2701 aactctgaca aaataacatc ctcaggcctg aatacattgt cacccactct gttgataccc
     2761 attgtagaga agagttccat aacaaaaagc gagttcggtt catcataaac ctctcttggt
     2821 ggcccttgct gaactacatt tccgttgtcc atcacatata ctctatctgc gactgccaac
     2881 gcatcttcgg gatcgttcaa aacaattaaa accgactccc ccagtgccat caacaaacgt
     2941 ttcattgtgg acctcagttc agttctgatc ttcttgtcga gtctttcaaa aggctcatcg
     3001 agcattataa tattgaattt cttcaccgtc tctctcgcaa atgctgtgag ttttttcata
     3061 cccgcaggta attcttttgg ccaacggtca agataattgg agaggccgtc tagttcttca
     3121 gctttcttag cgactttaac gtcggcttcc tcgtctctcg aaatcaaaag cggaaaggca
     3181 atgttgttgt gtgtgtccat gtgcggaaac agtgcgtttt cctgaaacac gaatgcgagt
     3241 ccccggagat ggggttccag aaaagtgaca tcctctccgt taatagtcac agtgccagac
     3301 agtggctctt gaagtcccgc aatcgttctc aaaagaagcg tcttaccgca gcctgaaggc
     3361 ccaattagcc cgactatctc ccctttttct acgaaaaggt ctatcggtcc caaattaaaa
     3421 gttccaattc tgcttcgcag gtcctttact tcgagagttc tcattagatc acctcccatt
     3481 aaagattata tctttgcctt aagcaaagcc gacggttttt tccttcaaag atatttggag
     3541 caggctttaa tagattggtt attatgtttg agaactggcg cttgtcgagc tatactatta
     3601 tcaaagctct atcaattcat cttttgtttt tctcaatgac attcggacag ccataattat
     3661 gaaaatacat ctgtatggga gtgaaaatag tgtctagaca tcttcttagt aaggccgaag
     3721 aataccggga agaactcacg agatttctta gcgatctggt tagcatccaa agcttctctg
     3781 ccggcgaaaa agaggtggtt aagagaatcg aagtggaaat gaacaaagtc ggttttgacg
     3841 aagtgaagat tgatgggctg ggaaatgttc taggaaggat aggaaacggc aagaaaatca
     3901 tcgccatgga tgcccatatc gacacggtag aagttgggaa cgaaaggtta tggaaggtcg
     3961 atccttttgg tggactggtt tctgacggaa ttatatacgg gagaggagct tcagaccaga
     4021 aagctggaat ggcagcaatg gtatacggtg ccaaaatcat gaaagaagaa gaacttcttg
     4081 gagacttcac cctgtacatt acaggaacgg taatggaaga agattgtgac ggcctctgct
     4141 ggagatacat tattaaagag gacggaataa ggcccgatta tgttgtcatt actgaaccga
     4201 caaatctgaa catttataga ggtcacagag gaagaatgga acttcaaatt agaacttcag
     4261 gtctctcgtg tcacgccagc gctcctgaac gaggaatcaa tgctatctat agaatgagcc
     4321 gaatcattca gcaaatcgag gttttgaatg aaaggttaaa agatgacccc ttcctcggaa
     4381 agggaacgat agcagtgact cagatcttct tcaagtctcc atctcaaaac gccgttgccg
     4441 acgaatgcac catacagatt gacaggagac taacagcagg cgaaaccaaa gagacggtta
     4501 tagaggaact caaagacgct ataagaattt caggcgaaga ggcggagatt ttggagcttt
     4561 tctacgaaag accttcgtac actggacttg tctatccagt cgagaagtac tttccgacat
     4621 gggtaatgga tgaaagtagt gaaattgttc agaagacagt aaatacttac aaggaggtct
     4681 ttggagaaga gccctttgtc gataaatgga ctttctccac aaatggaatc gcaacggccg
     4741 gtgtctttgc aattccgaca gtcggattcg gtcctgcaaa tgagattttc gctcacagcc
     4801 cggacgatca gtgccctgtg gatcatctag ttaaagcggc ggccatgtat gctcttcttc
     4861 cattaagact ctctcagtag attaggaggt aacataatga gtagcatttt gagaggcaaa
     4921 gacttcatta ccacacaaga tttcgacaga actgaaatcg accttatgct agatttatct
     4981 tctgatctca aaagaaggtt tgcaacaaat gaccccacac ctttgcttcc ttacaaaacc
     5041 gtgtttttga tgttcttcga tcaatctacg cgaactcgaa attcgatgga agcgggaatt
     5101 acacagcttg gcggtcacgc gcattatcta gatccatcga caatgcagac ggcacatgga
     5161 gaggttccga gggataccgc gatgatcctt tcaagatacg gtcacgcgat agcagtaaga
     5221 cattgcaagt tcaaagtcgg gaattcctac cttcgatctc ttgcagaaaa tgcagatgta
     5281 cccatcctga atctccagga tgacatctat cacccgatgc aggtcatggc tgacataatg
     5341 acaatgcgcg agagattcgg agcaaatctt agcggactga aagttgccat cacatgggct
     5401 tatgctgaaa gccatttgaa gcctctttcc gttccccagt cacagattct tctttttaca
     5461 agatacggaa tggacgtcaa actggcatat cccgaagggt ttgacttaat gccggatatc
     5521 gttgacctgg cgaagaagaa tgcagaagga agcggtgcca ggctggagat acttcacgat
     5581 atggatgaag ccttcagaga tgctgatatt gtgattccga aatcatgggg agggttctat
     5641 gtttcagacg atccccagga aataatgacg gaagtgagaa gaaacaaaga ttggatatgc
     5701 actccagaga gaatgaagct ggcaaacaag agggccattt acatgcatgc actccccgcc
     5761 gacagaggca gagaagtagt ggatgaagta atagacggcc cacagtcagt tgtcatagac
     5821 gaagcagaaa acagattgca cacggccaaa gctatcatgg ctcttacaat gggtggaaga
     5881 tcgtgactta tgaccttgcc ctcgaaaatg gaacggtatg cacggagaat ggactctttc
     5941 ctgcaaacgt ttacgttgag ggtgaggaaa taagagtgat ttcctcgatt aaagaaaagt
     6001 tgccggcaaa ggagagtatc gactgctctg gtctcttcct gcttcccggg ttcatagatc
     6061 ctcatgtgca tctaggcctg gctcttggaa gatacacttc tgccgataac tttgaatctg
     6121 gatcggctct tgctctgagt ggtggagtaa cgacgcttat cgactttctc gatccaatct
     6181 ccgatcttgc gcaattagaa gctgctaaga gagatagaat gacagcagct aaggacagca
     6241 gagtagatta ctcatttcac gtaaccttgg ctggcaatcc gagcttctcc gcggaagaaa
     6301 tcgctagatc gtcagtagcc gccaagatgc cctcgataaa ggtcttcact acgtatagct
     6361 cttccggtag gcgttcgagc gatgggatga tttacagtct gctttcactt tcaagaaagc
     6421 ttggattcgt tttgatggcc cattcggaga acgatgagat aataagagaa agggaaaaag
     6481 aattctccag gccaacctac agagatatct catctcttag gccgactatt agtgagcttt
     6541 ccgaagttgc aatgctttcg cttatctctc tggaagaaga aggacagctt tatatcgttc
     6601 atgtttcaag cggaaaaaca gttgaagctc tttcttccct tggcctcgac tggagaaggt
     6661 tcacaaaact tgaaacctgt ccgcagtacc tactactaaa taatgatcca ctaaaaaccg
     6721 accaaggata cctgaacaca tactgcccgc caccgaggac agatcaagag cgcgctcttc
     6781 ttcaatctgc gcttcgtaaa ggagagatat cttctatcgg aacagaccat tgcccgttcc
     6841 tcaacaggga aaaatgggaa aatcgagacg attaccccac gatacctaac ggtactggct
     6901 cgctgggtat ttccttcgca accatcaaca cgatcctcga cggtgatctc aaaagggttt
     6961 cctcacttct ttcgacaaat ccagcgagaa ttttcggcct ctttccaaag aggggagttc
     7021 ttgccccagg aagcattgct aatatcgcca ttgtcgatag agatgtgcaa agcactgtat
     7081 cgaggtcacg gtacacacgg tcagaccact cattgtttga gggtataaaa cttagaggtt
     7141 ccgtagaaac cgtaattcac agagggaaac tggcctttaa gaacggtgac atccttttac
     7201 cgaaagcctc gggacgcttc attgaaagag agaacattta ctgggagaaa ggcacatgca
     7261 aataaacaac gtttcgattt atacaaatga tggagattat attccaaagg gattcgtaag
     7321 ggtagaaaat ggaaagatag tcaaggtagc agccggagaa agcgcgtata acggcgaaaa
     7381 cttctatttc gaaggtaagt tgctcatgcc atctctagtt aatgcccata ctcacatata
     7441 ttcaacgttc gcaagagggt taaaagtagc tccattcaac acttcgtctt tcacgaaatt
     7501 actcgagcaa ctgtggtgga gactggatag agcaatgaca gtagaagatc tcaagatctc
     7561 cgcctatgtg gctgccatag aatctcttaa gagcggagtt acatctctct ttgaccataa
     7621 ttcttctcca aatgcaatag agggttctct tcgaggtatc gcttcttccg tgaattccgt
     7681 tggcctaaga tactgtggag cttacgaggt ttcggacaga gacggaacgg agcggcgcga
     7741 taaagggata agggagaatt tggattttag cagcgaaagc tcggcataca gaaagggcat
     7801 attcggactc cacgcctcct tcactttatc taatgaaacg ctttcccttg cttcaagaga
     7861 gatagcagga aagattccca tacatgtcca tgtcgcggag ggacctgaag atcaggaata
     7921 cacagttagt gttcatgact cgagagttct ggaaagattt cgcagggcag gactgatgac
     7981 cgaaaactcg atctacgctc actgcatcca cactacacct tccgaaagga ctcttgtcaa
     8041 agattcaaga ggatacatcg tcgtaaactg tcaatccaac gtgaacaatg gcgttggctt
     8101 tccagaatgg agaaactttg ctaatgaagg cataagaata cttctgggaa atgacgggta
     8161 cgggttcaac ctgtgtcacg atgttcggtt cctgatcctt tgccctcatt acatttaccg
     8221 tgatccgaaa gttagttctt caggtgatat cggccgttcg ctttttggct caaactacga
     8281 tctggcaaca aggacctttg gcgtaaatct tggtacaatc agggaaggga gcagcgcaga
     8341 cttcatcctt ctagattata agagcccgac tgaaatcacg gctgagaact tcgtcgacca
     8401 tttctttttt ggtatatgcg aaaacattgc agtcagcgac gctttcgtag gtggtgaacg
     8461 agtactggcg gaaggaagac ccacaagagt taatgaagat gatatataca aagaggctag
     8521 aaagctttac aaagcttttg aaaaaaggtt ctgaggaggt gcctggtgaa actcgctact
     8581 gagatagccg ggattcatct agagaatccc cttatgcccg cttccgggcc actaaccggc
     8641 gaccagagaa aaatgcgcgc aatagaggca atgggagtag gtgcaatggt aacaaaaaca
     8701 atatcaacgg ttgcagccaa ggttcccaga ccatgcatta tcgcagaaag ggacttcgta
     8761 ataaacacag agctttggag tgaattctca cctgaaaaat ggcttaatga atttcttcca
     8821 gattacagga agaaatcagc gcttccttta atcgttagcc taggctattc accagaggac
     8881 ttaagaaagt tggtcccacg attcgccaaa ttcgcagatg cttttgagtt gtcaactcat
     8941 tatgttgccg atgatccgtc gcttatcaga gagcttatct ctgcagtaaa agcgggaact
     9001 gaaaagcctg tctttctgaa attcgatcct tccgttcccg atccagcgga gatggcgaga
     9061 gttgttcagg aatcaggtgg tgacggaatc gtcgctatca attcactggg tccggtttac
     9121 cccatggacc ggagagtaaa tcgttcttat ttaggaagca gggacggttt cggttggatt
     9181 agcggaccgg taataaggcg gctatccctc tcatcggtga gaagaattcg tgaaggttgt
     9241 actctcccta taattggagt aggcgggatt gtcagcgcag acgatgtcgt agacttctta
     9301 tgcgccgggg cttctgcagt tcaaatgttg tcgggagccc tgataagggg aaaggggata
     9361 ttcaagagaa ttctcgaatc gcttccctcg gctctggaaa agagaggttt taaaagcgtt
     9421 gaagaagcaa ttggctctgc aaaggaccag aaagagtcct ttaccgtaag aaatcctgta
     9481 attgatcatg ataagtgcac tagatgcggt ctttgcgtgg aggtctgccc gtattttgca
     9541 ctcagtcttc aggaaaaagt ggaggtagat tcagcggaat gctttggttg cggactttgt
     9601 gaatccattt gcccagttgc tgcaatcgga ggtgtcctgg tttgagcaag tttcttctca
     9661 aatgtataac atgtggaaag gagttttcag aagaggaaga ggaatactat tgtccagttt
     9721 gcggcgagcg tagaggaacc ctggaagtcg tttacgactt tgatcaagtg agagaaaaat
     9781 acgagtcttc ttcggtcgat ctcaacaaca ggggtatttg ggcatttgat tttcttttgc
     9841 catttgagaa ttgtaccgat actccaaggc tcttcgtagg caatactcct ctatatagaa
     9901 accatttttt ggaagaaaaa tgcagagtat cggagttact ccttaaagac gacggcagga
     9961 atccgactgc ttcatttaaa gatagagcaa gcgctgttgc agtcttaaag gcgaaagaaa
    10021 agtctttcga taccgttttc tgcgcttcaa cgggaaatgc agccagctcg ctttcgggat
    10081 ttgcagctgt tagtggactg aagagcgtga tcttcgtccc cgctacagct cctgccgcca
    10141 agattactca gctacaggtg tatggatcaa aaatcctggc aataaatgcg agctacgacc
    10201 gggtattcga tttgtcgatg gaaatcggta tgtcaaaggg ttggtactgt agaaatagcg
    10261 caataaatcc ataccttctt gaggggaaga agacttgtgc aatggaaatc gcattcaaga
    10321 cgaaagacag actaccggat tttgtttttg tttcggcagg tgatggtact gtcttgtcaa
    10381 gtttctacaa agggtttgaa gatttgatga agctcggtgt gatctctaaa attccaacca
    10441 tagtgggagt tcaggcagaa ggttcaaacg caataatgag agcttatgaa agaggatacc
    10501 catttgagcc agtagatatc gaggcaagaa cggttgcgga cagcatcagt gtgggaaagc
    10561 caagagacgt ttttaaggct tgcaggtaca cacagaagca taatggtttg tttctttccg
    10621 tttcggacgg tgagattctc gggagtataa ttgaacttgc cagagaaacc ggtgtgttcg
    10681 ccgaaccggc aggcgctacg gcatttgcag gcttcaagaa ggccagaaga ttgagtattg
    10741 tgaacgaaag aaatagtgtt gtcatcgtgg taactggaaa tggtttgaag gaccttaaga
    10801 gcgtttcata tgtgcttcca aaggtcgaga ctttgccacc ggtaaaagat gcaattgagg
    10861 aggctctaaa tgaagatcag cttcacactc aacaatgaac ggttatcagt ggatgttgcc
    10921 gaagatatga gactactgga cttcttgcgt gatgaactgg gcctaactgg aacaaaagaa
    10981 ggctgtggcg aaggggagtg cggcgcctgt accgtaatca tcgacgggaa ggcagtcaat
    11041 tcctgtctcg tccttcttcc cgaaatcgat ggttccgagg taaccacaat tgaaggtctc
    11101 tcaaaggatg gggaactaga caccattcag aaggcttttg tagacgaagg agctgtccaa
    11161 tgcgggttct gtactccagg catgataatg tcggcaaagg cccttcttga tagaaacgat
    11221 tcgccaagcg atgaagagat aatggaggca atcgaaggca acctttgtag atgcacaggt
    11281 tactacaaga ttatccaggc aattaagatc gccgcagaaa acctcaggaa aactaatgga
    11341 aagtaattct gtttcgctga ttcttctggc ggcaggggaa tccagtcgct ttggagatga
    11401 gaagctctgt tctaatttca ggggaaagcc tcttctacaa tggtctctcg ataatctgaa
    11461 gaatgttggt gttcttaaca agtttctaat tattaagcct ggcttcgaac tcaatagatt
    11521 cgatacggga agcttcaaaa tattgataaa tgatgattac aaaaagggaa tttcgaccag
    11581 catagttaga ggactttgcg aatgtgctga agagcgcact ggaattgtac tcatgcttgc
    11641 cgatatgcct ttggttagcg gagaagatgt cgagtttctt ctggagtcac ttctccctga
    11701 agatgagatg gtctcctttg tgtatgacgg aagaaagggt ttcccgacat tcatagcaaa
    11761 gaagctattt cccgagctct tgaaaatcac tggagatagg ggtgcttttc aacttgttaa
    11821 gaatggacag gcgaaaatcc gtgaaataga aggcagtgag cgtaatgtat tcgatataga
    11881 ttcgccagaa gatagactgc aaatctggtg aggtgttctt aagattttgc taaaagcttc
    11941 attttgctcg agaaatccgg aggtgaggtt agtgtcattc aacgttctaa tgccaaccag
    12001 tgaagaagag ctagtttctc ttctttcgaa cgaaagcagt cacattctcg caggcggcac
    12061 cgaccttatc gtaaagataa ggttgaagag agtgattcct aagattgtag ttagccttaa
    12121 acatctaaag ggccttgaca caattgagga tctcggcgac aagataagag ttggctgcag
    12181 ggtaacgatt tccgaactct tgaagagcga aataatcgac cgacacggaa gaatcgtaca
    12241 gatggcctta agacaaatag ggagtccaca gattagaaac cgcgccactc ttgctggaaa
    12301 cattgttaat gcctcccccg ccggggactc gatagttgcg atggttctca cgaacgccag
    12361 tctgtcaata ggaagcgcaa atggtgagag gtatgaaaga gtggtggact tcataaaggg
    12421 tcctggggag acaactcttt caagaggaga attcgtaaag tcaataacat ttgagaagac
    12481 tgacgctctc ttcccgcagt tctacaaggt tggtcagagg aattctatgg ccatttcggt
    12541 cgcttccgtt ggatcgttga cttctgagtc ggaaattagg cttgcctttg gatctgttgc
    12601 gccgaccgtt gtcgtaccga tcgaggcttg tgaatactac tctgcaaaga acgggtcatt
    12661 tgacgaagag cggtttgtcg aactagctat gaaatgtgtg acacccattg atgatgttag
    12721 agcaaccgcc tggtatcgga ccactgtagt aagaaatctg attcatagaa ctctaaaagt
    12781 ctggagaaag aaggggtaca atgctgtcta atgtaaacga gtacttcaga cctaagacag
    12841 tcgaagaggc ttacgaactt atgcagaatg acagagatca ttcgattttc ttgtctggag
    12901 ggacaattgt agcacttatg gagtcctcaa gaattgaaaa gataatcgac ttgaagtctc
    12961 ttagacttga cacaatttca gttacggagg gtgaaatagt tgtcggagca actacgacta
    13021 ttgaatcctt tagaaaggat ccagttatca ggaaggaatt cgatgacttc ttctacaacg
    13081 ccttctcatt agtgggttcc tggcagatca ggaacatggc aacaataggt ggctcggtcg
    13141 ctccaaaact tggatggtcg gatgtgagca cctgccttct aacaacgggt tctagtctta
    13201 tgatctatgg ggtagacgga tatcagagga tgttagtatc cgactatttt tctttaccta
    13261 caggagaaaa gcctctaatt acccatgtaa tgttgaagag agacggctgg gtttcggcct
    13321 tcgaaaagtt ctctaagact actttcgaca ttgcaaccgt aaatgttggt ctctcgatta
    13381 ttcccgaacg taaccgaata aagggtgcca gggtcgtatg tggatcgaga cctcagtatc
    13441 cagcgagatt cgagagcgtt gaagcggcaa tgacagacct ggagataaac gatgtaatgc
    13501 ctgcgatagt tagaagcctc gtttacgaaa gcttctctgg aggatctaac atgaccgctt
    13561 ctttcgagta tagaaaacat ctcgcgtccg tccttgccca gagagctgcc gaaaaggtta
    13621 aggagatgat cacatgaaaa tagctcttac agttaacggg aaacttcatg atatcgatat
    13681 cgatccagga gaatacctgc tcgatgtact gagaagaatc ggctacaaaa gtgtcagaag
    13741 aagctgtaat tcggcaagtt gcggcacttg cacagttctc ttgaacggca aaccaatact
    13801 ctcttgttct acttttgcag cctcggttga cggtcaaaac atcgagactg tcgaaggaat
    13861 ggcagatgga gaaaagcttc acccaattca agaagctatt ctcgaagaag ggggtgctca
    13921 atgtggatac tgtatccctg gactaataat ggcaggagaa gctctgctaa gagagaatag
    13981 cgatccctct gacgaagaga taaaactcgc gctaaatggc aatatttgta gatgtactgg
    14041 ctacgaaccg cagaacagag caatcaagaa ggcagcaaaa gtgatgagag gtgaactgga
    14101 tgactgattc cttcgtgacc gtaggaaaac caaaaaggaa ggtggatggt cttgcaatag
    14161 tcagaggtaa accggtatac actccagact acgacatgcc agacgctcta gtcgtcaagt
    14221 tacttcgctc gcctcatgcc cacgctttag taaaaaatat tgaaacggag caagcaaagt
    14281 taattaacga tgttgtggat atcttcactt tcagtgatgt cgagaggata ccatatacaa
    14341 gggccggtca gggctatccc gaaccgtcgc cgtacgacac ctttctcctg gatagaaagg
    14401 ttagatacgt aggtgatcca gtggcaatcg tagctgcgaa gactgagaaa gcagcaaaag
    14461 acgcactgag aaatatcaaa gttgagtatg aaatcctcga tgccgtctta gaaatgaaag
    14521 aagctacaaa agagggggcg ccgatcattc acgatgaaag cgaatgtgct ggagtctacg
    14581 acaaatccag aaacattgcg gcgcattatg aaatggatct cggagatgtc gaagaaacgc
    14641 tcaagacttg tgatgttgtg atcgagagga cttatcatat cgacacacag cttcacgcag
    14701 cgcttgaacc acacaacgcg cttacatatt tggactccgt cgggaggctc atagtaatca
    14761 cttcaactca agtacccttt ca
//
LOCUS       DAOQYI010000037        14127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_4788_length_14127_cov_66.081154, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000037 DAOQYI010000000
VERSION     DAOQYI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 14127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14127
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4788_length_14127_cov_66.081154"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PKL93_08665"
     CDS             <1..1191
                     /locus_tag="PKL93_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ71368.1"
                     /translation="MMKDLLREQLIRIVQEYGADILKDPGKVKGLLKDFCPEEPRGKI
                     FAITQCLREGIHRELIDSSKRKTIRIELSRLIRKIHDNFGLKEDISRWAVETIALALG
                     IISEKETVITTERSQVIEQKRVETHKVISHERIVEERGKRTTNGDELIYVERGSFIMG
                     DTWGDGFPYEKPTHKVTLTYDFHIGKYPVTFEEYDKYCMRTSAKRPGDFSWGRGRRPV
                     INVSWWDAISYCNWLSEKEGIPKAYDDKGNILDKYGKTTTDITEVVGYRLPTEAEWEY
                     AARGGNKSKGYKYSGSDNADDVAWYASNSKGMTQEVGTKLPNELGLYDMSGNIWEWCS
                     DFYGGYSNMRKTNPFTFKGSRRVSRGGAWYSEVSDLLILCRNAILPEKAPYYVGFRLC
                     LTVS"
     gene            1417..2412
                     /locus_tag="PKL93_08670"
     CDS             1417..2412
                     /locus_tag="PKL93_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71369.1"
                     /translation="MRIKLRLFFVCSYLLMSAVFFSFVSKVTSINDYSSIYEEESGYR
                     VDISYPGPQLFWTLITLDDNRDFFKRVYIVDQSASVHIEHVPRQMDITIDSFSGDKTV
                     SVSKSIFKKAPKSDLSFLLLVMGIPIAALLAIIGVVIMVRYNRKILDKASRILERSKT
                     KDAIGKMESRRTALIALWFTVLVIGVGVIGVSYYLGLFPSDPVITQEGALSFPDERPN
                     SLPEAEFSFLKETFNKLSDGGPKVIERYFQLNHDLEEVKKTPIQNEEKSKTISRIVEE
                     LEKIDPAIYQIVEALQTIKLKVINGQITSNEDKKEIDRLLITMDEINKEPPAYNF"
     gene            3272..8104
                     /locus_tag="PKL93_08675"
     CDS             3272..8104
                     /locus_tag="PKL93_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HNQ71370.1"
                     /translation="MENNDAVSVKVDKWKKALLDLTMRNRLINFRTSKTSNLMLIRPS
                     SSEIFNKIVVQEKEMNFVPIPPEDDEEQEYRSIVSLRANQIQADREEKEMNLVLNRLK
                     LKSRTSYEEMGINTLFMTFGFLKWKEIDYNKSYVISPLLLVPVSISRETIASPYSVRI
                     IDDDIVLNPTLSEKLEEFGLDLESAYDQEISLEESTLRDLFVKVRNLIEPIEGWSVAE
                     DVALGVFSFAKLAMYKDLENYANMIYENAVLRTIAEGKPLVVPDNENDELADLDVEND
                     PLHCYQILDADSSQQEAILAVKSGQDLVIQGPPGTGKSQTIANIIAESLSQGKRVLFV
                     SEKMAALEVVKRRLDDCELGDFCLELHSKKTGKRVVLDELDRVLNEANNHAVDTRSLE
                     RELSRLKEIRERLNSYVRRLHEKRTSLEISAFELHGNLAALHDIDLMSFDLKHVQDLT
                     RDQLEKIISNLDNVKRHAEMYRNHKKDVLNKVRAVAPDAVFVMRVRQSLAKIRGAIGK
                     LREIGEDTSLEWRSSISKELEYANSRISAILDLFEAESSAEDKALKLAEDLLSHILPI
                     TRDLPSSYRDSFLNLSSAIDEVKDSHSEVFSQFNTLRPLLGITNMLKSVRSFEETVSL
                     LTSYRPDILQLNLTRLQEKFARSYNSFFGRLFGGYKKNLRILSSLKYDGIVDTETIVE
                     DLTKASRVLSQCGIMTKACDKEELISVLERSKKTIRSFYESYSKFEKALRNSESDLRE
                     LNEYFSFFSILPNGTQNQQSIPGKSYWVQRVISATNVFFKDLEEVSDMVITEPFSDGK
                     SLLDFEGFEDFVHRLQDSLPKLDDWLSMKKSIIFLEKAGLEDFLERARESEVETDMLD
                     KVFLKGFYSRLLSELYANDEVLNSFSTNDHNLLVKEFRILDSKQQLYARKRLASLLAQ
                     RVPVSNLVNSGSAETSILRREIAKKRRNKSIRRLFSEIPNLLQVLKPCMMMSPLSVSV
                     FTNPERFRFDVAIFDEASQVFPEDSVGAIMRAKQVVVVGDNKQLPPTSFFRISEPDEA
                     ELTDEENDLESLESILDECSTAGLPEKKLLWHYRSRHESLIAFSNYHFYMNRLSTFPS
                     SSFDGLDSGICFEYVSNGVYDRAKSRKNIEEARKVAEMVMSHARTKPGVSLGVVAFSE
                     AQQTAILDRIEELRSQDQSVEDFFGEDKLEPFFVKNLENVQGDERDEMIFSIGYGRDA
                     SGKLTMNFGPLNKNGGERRLNVAITRAKKKVTVVSSIRGGDISAEIQTPGVRALREYL
                     NYAEQGGSKEFLLRETENTGGEFESPFETEVYEELTAREFNVHKQVGCSGYRIDLAIV
                     DNEHPGRYIAGIECDGAMYHSARTARDRDRLRQQVLEKLGWKIIRIWSRDWIFDRNSE
                     MERVLAEIEAARSVSENTEVLHINNELIIEDPLPSIEREEVTLPIYEKFSVFDLTRML
                     SSKRAVQVFDAVRAIIEKEAPVHLDEVVSRISMLFDTHPHLLQVNWDMYTKSGNISRK
                     NLREFVMTQFPRDSFVLQGDFLLKRNERILPRQSASRNDMRKPEHIPPVEIQEAIKLC
                     LKNAFTMDREDLKTDVARLFGFKRTGTNISTVIDNEVSLLVRNHEIQVQDGKVSIRKA
                     "
     gene            complement(8232..8588)
                     /locus_tag="PKL93_08680"
     CDS             complement(8232..8588)
                     /locus_tag="PKL93_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71371.1"
                     /translation="MHAIDENGRTVLLNEDGSWQYENSKEWRVVRTYMGDSTDKNTDL
                     FEIHHPQWRVRVSTEDSVSIYWHEPSSGNQDDLIDMGSKGSDESYVYKNGSFYLEIFA
                     SSGKYRIVVEEKVPAE"
     gene            9058..9543
                     /locus_tag="PKL93_08685"
     CDS             9058..9543
                     /locus_tag="PKL93_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ71372.1"
                     /translation="MDIDEIIIYDNILMKVSDESNLEGGVKMTGFDKLKANLMKNEEF
                     ARLYNERKPLRNFIDELIELRVEKGMTQKDLSELTGISVPNISRIEAGKQNLSYQMMY
                     KLVSALEGKILLTAQADNCIRLSNVAAEKLQELSKEANVSPEEFLMNLLQKVSEEAIK
                     V"
     gene            10197..10736
                     /locus_tag="PKL93_08690"
     CDS             10197..10736
                     /locus_tag="PKL93_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71373.1"
                     /translation="MKRMSVSIEGFDLSGLVETIKQAFTAPSSEPESLREMSPYDEIV
                     YGFDYSEFPRRWSKEEEPDIVKTIMEGKLLVFLPFEVSCFEDEILFCVDGTTIDIPKS
                     KSQDPDGESLLEIIRDESNVIGFCVLRKDGHLLIESCVHHKTCANNLVPFLKPLKFCG
                     FIEVPMHRFLNDFILRSLD"
     gene            complement(10784..11293)
                     /locus_tag="PKL93_08695"
     CDS             complement(10784..11293)
                     /locus_tag="PKL93_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71374.1"
                     /translation="MEENHFSERGEFTEMVRRILDRFLQDVPEAAYIGQEEQPFLQED
                     RRHGVRPEEIPGIIGDVTGLSDFNCYPGYPGNICYPVAFFVSLNSSVVAKGRGHLHFQ
                     KILDCFIEHMLVKCDGITKNAIMVTDSWDARIAYSKRSFIDKIRKQANVELYILSLYP
                     AKRIVRFPI"
     gene            complement(11460..12113)
                     /locus_tag="PKL93_08700"
     CDS             complement(11460..12113)
                     /locus_tag="PKL93_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71375.1"
                     /translation="MYSFEGVKKILETSIDSLVDNFVREPYIHRCEHSLHCELYAMLA
                     SHRALQGLYPIGSSGRKASLVHKEWPEPFTREGHTRRGNVDLAILSDKDLAHPLESSR
                     ENDVFPSNATNLSEIDIFTGGYLKPDFVVEIGLNYPFEHLESDHKKLLNSRYSKGNGN
                     KEAYLVHFWQPHNTAALDDIKEIQSLILKADVNIAAVIFTKSEILIKHLSDKDLSPI"
     gene            complement(12393..13934)
                     /locus_tag="PKL93_08705"
     CDS             complement(12393..13934)
                     /locus_tag="PKL93_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNQ71376.1"
                     /translation="MKEKDTASNLVSAANSLDAVANVEPVRPAMETVLDTERQKDADI
                     LLLRNGDKLTGTVVNEKFSITTSYAHLDVVTKHIAGMNLEGGANNIESIIAENNNRFS
                     GFIDDSFISFKLTNGPQLEIRREKVLKIVLRQRSQDMGEMKWTPFIWLKNGDYFHGQI
                     LTKDIILSTIYAKIPLDFATTESITLIGESNPLLTIVKMTNGDSVHGILEIEDIQIEL
                     DAGSVIEVYQDRIDRIFFKEGHVSAVATKNHFALVEKGSFTMGNTWGDGKSNEKPTHK
                     VTLTYDFYIGKYEVTFNEYNAFCEATGRSKNSDDGWGGGIRPATYVEWFDAIEYCNWL
                     SEMEGLLKAYDSEGNFLDKDGRVTTDPSKVVGYRLPTEAEWEYAARGGNKSRGYKYSG
                     SDNVDDVSWCRRNSGDEYLDENDIDKFIGNKCKIQKVGKKAPNELGLYDMSGNVEEWC
                     SDFHSSYSCSPKTNPYNSTNSSNRVIRGGSYQSHASETTVASRSYCNPTHRGGFIGFR
                     ICKTL"
BASE COUNT         4232 a         2799 c         3128 g         3968 t
ORIGIN      
        1 atgatgaaag atctattaag ggaacaacta atcagaatag tccaagagta tggagcagac
       61 atactaaaag acccaggaaa ggtgaagggt ctcctgaaag acttctgccc tgaagaacca
      121 aggggaaaga tatttgcaat cacacagtgc ctaagagaag gaatacatag agagcttata
      181 gattcaagca agagaaagac gataagaata gagttgagca gactcataag aaagattcat
      241 gataactttg gactcaaaga agatatatcc agatgggcag tcgaaactat tgctctggct
      301 ctggggataa taagcgaaaa ggaaactgta ataacaaccg agagatctca agtaatcgaa
      361 caaaagcgtg tagaaactca taaggtaata tctcatgaac gaattgttga ggaaagagga
      421 aagagaacaa caaacggaga cgagctaatc tatgtcgaga gaggaagctt cataatggga
      481 gacacatggg gagacggttt cccatacgag aagccaacac ataaggttac gctcacatat
      541 gatttccaca taggcaagta tccagtgact tttgaagaat atgacaaata ctgtatgcgt
      601 acaagtgcaa aaagaccggg tgactttagt tggggaaggg gaaggagacc ggtaataaac
      661 gtaagctggt gggacgctat atcttactgc aactggttga gtgaaaagga aggaattcct
      721 aaggcgtatg acgataaagg taacatcctg gacaaatatg gaaagacaac tacagacata
      781 actgaagtag ttggttacag gttacccact gaagccgaat gggaatacgc tgcaagggga
      841 ggaaacaaga gcaagggata taaatactct ggtagcgata atgcagacga tgttgcctgg
      901 tatgcttcta actcaaaagg catgacacag gaagttggaa ccaagttacc taacgaactt
      961 ggtctgtacg atatgagcgg taacatttgg gaatggtgta gtgacttcta tggtggttat
     1021 tcaaatatga gaaagacaaa tccgttcact ttcaaaggtt ctagaagagt tagccgcggc
     1081 ggcgcatggt atagtgaggt ttccgatctg ctaatactat gtagaaacgc tatcttacca
     1141 gaaaaagctc cttattatgt tggtttccga ttatgtctaa ccgttagttg atagtggttt
     1201 tcttactagt tacactctgc agtattgaca gaatcctacg cttcaaatta atcgccaagc
     1261 atttcaagta acaagcatca agattattgt ctaatgaaat caagcagggc gataatgctg
     1321 cgctttgcct tatattttgc ctatcagaat tgcctttttc atttttgtgt tgccttattg
     1381 tataatgaat tgtaatactt tagaggcggt gagttcatga gaattaagct gagactattc
     1441 ttcgtctgtt cctatttgtt aatgagtgcg gtcttttttt cctttgtctc taaggttacg
     1501 agtattaatg actactcttc aatttatgaa gaagagagcg gttacagggt agatattagc
     1561 tatccaggtc ctcagttatt ttggacactg attactcttg acgataatag ggactttttc
     1621 aagagagtgt atattgtgga tcagtctgcg agtgttcata ttgagcacgt tccccgacag
     1681 atggatatca ctattgactc tttttctggc gataagacag tctccgttag caagagtatt
     1741 ttcaaaaagg cccctaagag tgatctgtct tttttgctgc ttgtaatggg tattcctatc
     1801 gctgctttgt tagcgatcat aggcgtagtg ataatggtcc gttataatcg aaagattctt
     1861 gacaaggcga gcaggatatt ggaaagaagt aagaccaaag atgccatagg taaaatggaa
     1921 tctagaagga ccgcgcttat tgccctttgg tttacagtct tagttatagg tgttggtgta
     1981 attggtgttt cctattactt ggggttgttt ccatctgatc cagttatcac tcaagaagga
     2041 gccttatcat ttccggatga aaggccaaat tccttgccag aagctgagtt tagtttctta
     2101 aaggagacct tcaataagct ttctgatggt ggcccaaaag tcatcgaaag atatttccag
     2161 ttgaaccatg acttggaaga ggtgaagaaa actccgattc agaatgagga gaaatctaag
     2221 actatttcta ggatcgttga agaacttgaa aagatcgatc ctgcaatata tcaaattgtt
     2281 gaagctcttc agacaatcaa gcttaaagta attaatggtc agattacatc caatgaagac
     2341 aagaaggaaa ttgacagact actaattact atggacgaaa ttaacaagga gcctcctgca
     2401 tataactttt gaaggtgctc aagagaaaca cgaatacttg cgtggattag tcattaccta
     2461 gaactaacaa gagcttctgt gacacgaaaa actttttctt ttagttcaag acgtgttcga
     2521 gctaggacta cttgagagtg taaataggaa cgattcgaaa gaattattaa gtgaaagtaa
     2581 ctcagatgat tgtggtacga agactacacg gcaaatatgc tatattcaac atttctggga
     2641 tcatttcata gttgatttca cgtatggtaa ttctcatttc tgtatagagc cgcttcgatg
     2701 tgtatgcatc acatggttat aatctttcta aaaaccagtc tacatgtact ggcgcatagt
     2761 gatgagatta tatagatagc atctttgaac ttcggtaata caaaacagaa agtttgacag
     2821 ttgagacaac tttcaaagtt gataaaatga agcatgtctc tagataattt tcaaagcttc
     2881 tacttctaag acttcgtcgg aaacaatctc tatcgtttct atctattagt agtcaaacaa
     2941 atgggatttt gagtgaccgc aaagaacctc ttcacattgt ttcatttgct tacaaaatga
     3001 tagaatctct ttagacaaat atgcgtaatg tagttacttc aaggtgcagt aatgatgaag
     3061 ctaataagtc taagcgaata atgctggatt tccggagaag ttcatcttga gtggcgtgtc
     3121 ccataagcga gtaagtgctg ccaacaagaa tggacggatc tctgatctga gtggacaaag
     3181 tctcttcaat aacttcaatt aacgttccgc cgatatattt ggtccgttct gtttagacca
     3241 gctgtgttag aaaggctttt ggagggatcc catggaaaac aatgatgctg tttccgttaa
     3301 ggtagacaag tggaagaagg cgttgctaga tctcacaatg agaaacagat taatcaactt
     3361 caggacaagc aagacgagca atttgatgct cataagacct tcaagcagtg agatcttcaa
     3421 taagattgtc gtccaagaaa aagagatgaa cttcgttccg attcctcctg aagatgatga
     3481 agagcaagaa tatcgaagca ttgtttccct tagagcaaac cagatacagg ctgaccgtga
     3541 agaaaaggaa atgaatcttg ttctcaatag actaaaactc aagtccagga cttcttacga
     3601 ggaaatggga atcaataccc tttttatgac tttcggtttc ctcaagtgga aagaaataga
     3661 ttataacaag agctatgtga tttccccttt actccttgtt ccagtttcca ttagccgtga
     3721 gacaattgcc tcaccctatt ccgtaaggat catcgatgac gacattgtac ttaatccaac
     3781 actttcagaa aagttggagg aatttggcct tgacctggag tctgcttatg atcaggaaat
     3841 atcgcttgaa gagtcgaccc tgagagattt atttgtaaaa gtcagaaatc taattgagcc
     3901 gatcgagggt tggtcggttg ccgaagacgt ggctttaggg gttttctcgt ttgccaagct
     3961 tgcaatgtac aaagatttag agaactacgc aaacatgata tatgagaatg ccgtgctaag
     4021 aacaattgca gaggggaagc cgttggttgt gccagataat gaaaacgacg agcttgcaga
     4081 cttggatgtc gaaaacgatc ctcttcattg ttatcagata ctggatgccg actcgagtca
     4141 gcaagaggct atccttgccg tgaaaagcgg tcaagaccta gtgatacagg gacctccggg
     4201 aactggaaag agtcagacaa tagcgaatat catcgcggag tccctgagtc agggcaagag
     4261 agtcctcttc gtaagcgaga aaatggcagc tcttgaagtt gtaaagagac gtttggatga
     4321 ttgtgaattg ggagattttt gtctggagct tcacagtaag aaaacgggga agagagtcgt
     4381 cctggacgag ttagatcgcg tcctaaatga agctaacaac catgctgtag atacgcgaag
     4441 tctagagcgt gaactctcta gactcaagga gataagagaa agactcaaca gctacgttag
     4501 aaggctgcac gaaaagagaa cttctcttga gatttcagca tttgagcttc acggcaatct
     4561 agcagctctg catgatatag atcttatgag ttttgattta aaacacgtgc aagatctcac
     4621 gcgggatcaa ctcgaaaaga tcattagcaa tcttgacaat gttaagcggc acgccgaaat
     4681 gtatagaaac cataagaagg atgtccttaa caaagtcagg gcagttgctc ccgatgctgt
     4741 ctttgtaatg cgcgttcgtc aatcgcttgc gaagataaga ggtgcgattg gaaaactaag
     4801 ggagatagga gaagatacat cgctcgaatg gagatcttcc atcagtaagg aactagaata
     4861 tgcaaattcc agaatttcag cgattcttga cttatttgag gccgaaagct cagccgaaga
     4921 taaagccttg aagttagcag aggatcttct ttctcatatt ctgccaatta caagagatct
     4981 tccgtcaagt tatagagatt ctttcttgaa tctaagtagc gcgatagatg aagtcaagga
     5041 ctctcacagt gaagttttca gtcagtttaa tactcttcgc ccgcttcttg gaatcaccaa
     5101 tatgctaaaa tccgtaaggt cattcgaaga aacagtgtcg ctgctcacta gctatcgacc
     5161 agatattctc cagctgaacc taaccagact tcaggaaaaa ttcgcgagat catacaatag
     5221 tttctttggg aggctgtttg gcggttacaa gaagaatctt cgtattcttt cttcattgaa
     5281 atacgatggg attgttgata ccgagactat cgtagaggat ttgactaagg cgtcaagagt
     5341 cctctcacag tgcggaataa tgacgaaagc ctgtgacaag gaagagttga taagtgttct
     5401 cgaaaggtca aaaaagacaa tcaggagttt ctacgagagc tatagtaagt tcgaaaaagc
     5461 tttgagaaac agtgaatcag atttaagaga gcttaatgaa tacttctcat tcttttctat
     5521 attaccgaat ggaacccaaa atcagcagtc gattccgggc aagagttact gggttcagcg
     5581 tgtaatctca gcaacaaatg tgttcttcaa ggatctagaa gaagtatccg acatggtgat
     5641 cacggaacca ttttctgacg gcaaatctct cttggacttt gaaggctttg aagattttgt
     5701 ccatagactc caggattcgc ttccaaaact tgatgattgg ctttcaatga agaagagtat
     5761 catttttctc gagaaggctg gactggaaga ctttctcgaa agagctagag aaagtgaagt
     5821 ggagaccgat atgcttgaca aagttttcct aaagggtttt tacagcaggc tattgtcaga
     5881 gctatatgca aacgatgagg ttctgaactc attctcaaca aatgaccaca atcttcttgt
     5941 taaagagttc aggattctcg actcaaagca gcagctgtat gccagaaaga gactggcttc
     6001 tttgcttgcg cagagggttc cggtttccaa tcttgtgaat tctggaagcg ccgagacatc
     6061 tatcctgaga agagagattg caaagaagag acggaacaag tccataagga gactgttctc
     6121 ggaaatacca aatctgctgc aggttctgaa accatgcatg atgatgagtc cgctatctgt
     6181 aagcgtattc accaatccag aaagattcag attcgatgtc gccatttttg acgaggcctc
     6241 ccaggtattt ccagaagact cagtgggagc aatcatgagg gcaaaacaag tggtggttgt
     6301 aggagacaac aaacagctac ctccaaccag ctttttcaga atctctgaac ccgacgaagc
     6361 tgaactgacg gatgaagaga acgatctcga gagccttgaa agcattctcg acgaatgcag
     6421 cacagccgga ctaccggaga aaaaacttct ctggcattac cgaagccgtc acgaatcgtt
     6481 aatagccttc tcaaactatc atttttacat gaatagactc agcactttcc ctagcagcag
     6541 ttttgatggg cttgattctg gaatctgctt tgaatatgtt tcgaatggag tttacgacag
     6601 agcgaagagc agaaagaata ttgaagaagc tcgcaaagtt gctgagatgg ttatgagcca
     6661 tgcaaggaca aagcccggtg tttcattagg agtagttgcc ttcagtgaag ctcagcagac
     6721 tgcaatcctc gaccgaattg aagagctccg ttcccaggat cagtctgtcg aggatttctt
     6781 cggtgaggat aaacttgaac ctttcttcgt gaagaatctt gagaacgttc agggtgacga
     6841 gagagatgag atgattttca gcattggata tggtagagat gcttcgggaa agttgacaat
     6901 gaactttggt cctctaaaca aaaatggagg agaaaggcga ctcaatgttg caataactag
     6961 agccaagaag aaagtcactg tcgtcagttc aataagggga ggagacatct ctgctgaaat
     7021 acagaccccg ggagtaagag ctctaagaga atacttgaac tatgccgaac agggaggaag
     7081 caaggaattc cttttgagag agactgaaaa cactggcggc gaatttgaat cgccctttga
     7141 aacggaagta tatgaagaac tcacagcacg cgaattcaac gtacacaaac aagtgggatg
     7201 ctccggttac aggatagacc tggcaattgt tgataatgaa catcctggaa ggtatatcgc
     7261 aggaattgaa tgtgacggag ccatgtatca ctccgcgaga actgcaagag atagggacag
     7321 gcttcgacag caagttcttg agaaattggg ttggaagatt attagaatct ggtccaggga
     7381 ttggatattt gacaggaatt cagaaatgga aagggtcctt gcagagattg aagctgcaag
     7441 atccgtttca gaaaacaccg aagtacttca catcaataat gagctgataa tcgaagatcc
     7501 tttgccatca attgaacgag aagaagtgac tctccctata tacgagaaat tctcggtgtt
     7561 tgatctaacc agaatgcttt cgtccaagag ggctgtgcaa gtttttgacg ctgtaagagc
     7621 aataattgaa aaagaggccc cggttcattt ggatgaagta gtgtcgagga tttccatgct
     7681 tttcgatact caccctcatc tactccaggt aaactgggat atgtatacga aatccgggaa
     7741 tatctctcgt aagaatctac gagaattcgt tatgacacaa ttcccaagag actcattcgt
     7801 attgcaggga gatttcttgc tcaagcgcaa cgaaaggatc cttccaagac aatcggccag
     7861 cagaaatgat atgagaaagc ccgaacacat cccacctgtg gagattcaag aggccataaa
     7921 actctgtctt aaaaacgcgt tcacaatgga tagagaggac ttgaagacag atgtggcaag
     7981 actattcggt ttcaagagga cgggaacgaa catttctact gtcatagaca atgaagtcag
     8041 cttgcttgtt cgaaaccatg agattcaggt tcaagatggg aaagtgagca ttagaaaagc
     8101 ataattgggt gcataatgga ccctgggtca tctacaatgt gggttgccgt gttgagcaaa
     8161 tacaaatttg ctggaaatcc tgaattcatg tgtttgcgtt attccgctgt ttcaaatgaa
     8221 gggcataaca cttattctgc cggaaccttt tcttcaacta cgattctgta ctttccggat
     8281 gaagcgaata tttccaagta gaaagatcca tttttgtaaa cgtagctttc gtcagaacct
     8341 tttgaaccca tatctattag atcatcttga ttacccgatg aaggttcgtg ccagtaaatt
     8401 gaaacactat cttctgttga tacacgtacc ctccactgtg ggtgatggat ctcgaagaga
     8461 tccgtattct tgtctgtcga atctcccata taggttctta caactcgcca ttcttttgag
     8521 ttttcatact gccagctgcc atcttcgtta aggagcactg ttcttccatt ttcgtcgatt
     8581 gcatgcatgc taggatcctc ctaattagat gccacttggg caccgattta aataatcgaa
     8641 aacatactag agcaagacgt taggtttggg ttgggtgtga cacaggggaa aaagatgaaa
     8701 tatttagttg aaacaataca taactggtgg cggctcgcca tctcaatgat cacaatcgtt
     8761 ttggtagcgc attcgacgta cagttacagt aagtctagca tattgagcaa tgtggggatc
     8821 aatcgatcac aattgacaga ccagttaatt atcatcactt agctatactt ttgggcagaa
     8881 gtcgcattct gaattgcttc aacactagag aataaacagt tgatcccggg ttctggattt
     8941 ttatttattt gtttggccaa atactgtatt cctagttgcc atagacaaat agaggaatag
     9001 tggaactaaa agaaatgttg ttttgttctt gtggttatag aaaagagaaa gggtaagatg
     9061 gatattgatg aaattatcat atatgataat atacttatga aagtgagtga tgaatcaaat
     9121 ctagaaggtg gtgttaagat gacaggattt gataaactga aggccaactt gatgaagaac
     9181 gaggaattcg cacgcttata caacgagaga aaaccattaa ggaacttcat agatgaacta
     9241 atcgaactca gagttgagaa gggtatgacc caaaaggatc taagcgagct cacaggtata
     9301 agcgtcccaa atatctcaag aatagaggca ggaaaacaga atctttccta tcagatgatg
     9361 tacaaactcg tgagtgccct tgaaggaaag atcctactaa cagcccaggc tgataattgc
     9421 attagacttt ccaacgtggc cgctgagaaa ctgcaagaac taagcaaaga agctaatgtc
     9481 tcgccggaag aattccttat gaacctttta caaaaggttt cagaagaagc gattaaggtc
     9541 taggtgaaac tcaaagggtt cataacttgc ccggttctaa atatctctac caatagtgag
     9601 tctaccgcaa ataggacaat gagtgtgctc gaagccatgt gaatgtttgg atataaatat
     9661 atgacttgag caaacaaata cggaaagaaa gcgaagaatt cttcagcagt gatcagcatt
     9721 cttcttcatt gaacgtagaa ttggttttcc tgaatacgag tgagaccatt ttttctggac
     9781 aagtatcaat caaaattgga atggacagat aaaagtacat aggagtcaac ttcaacgatc
     9841 cggctataga ataccatgtc attattagct tattgatgaa aacctagagc agagtattta
     9901 ggaattatcg ataagtgctc ggtctcagtg ataatacctt gcgcaatgtt gtactagcgc
     9961 gttaaggagt agtaagaggt tccgttaagt ctcttttaga atgactagtt ttaattgttt
    10021 caaggattcc gctgttattt ccaatcatag tgctgctatc cttttgttga ggcaatggat
    10081 ttggagatca tgcgatgcga ttctattatt agttcggtca gaagtcatca tataatagtt
    10141 ttactcaaaa gcatttggcc tcactaagtg aaaaccttcg gaggtggatc tcaaggttga
    10201 agagaatgtc agtgagcatt gagggattcg acttaagcgg cctcgttgag actataaagc
    10261 aggccttcac cgctccttcc agtgaacccg aaagcttgag ggaaatgagt ccctacgatg
    10321 agatagttta cggatttgat tattcggagt tccccagacg atggtccaaa gaagaggaac
    10381 ccgatatagt gaaaaccatt atggaaggta agcttcttgt ctttcttcct ttcgaggtca
    10441 gctgttttga ggatgagatc cttttctgtg tagatggtac aacaatagat attcccaagt
    10501 cgaaaagcca agatcctgac ggcgaaagtc tccttgaaat aattcgggac gaatctaacg
    10561 tgattggatt ttgcgttctt cgaaaagatg ggcatctact aattgaaagc tgtgtacatc
    10621 acaagacctg tgcaaataat ctcgtgcctt ttctaaaacc acttaaattc tgtgggttta
    10681 tagaagtgcc aatgcatcgg ttcctaaatg attttatctt gaggtcattg gattaggttc
    10741 gccttgaaag cttctccgat gtgcattagt tattcccgtt aggttatatt ggaaaccgga
    10801 ctatgcgctt agcaggataa agactcaaga tgtacagctc tacatttgcc tgttttctta
    10861 tcttgtctat gaaacttctc ttactgtacg ctattcgtgc atcccagctg tcggttacca
    10921 tgattgcgtt ctttgtaata ccgtcgcatt ttactaacat gtgctctatg aagcaatcca
    10981 gaatcttctg gaaatggaga tgccccctcc ccttggcaac cacacttgaa ttgagcgaca
    11041 caaagaaagc gacggggtag cagatattac ctggataacc aggataacaa ttgaaatccg
    11101 acaggcctgt gacatctcca atgatgcccg gaatctcttc cggtctcact ccatgtctcc
    11161 gatcttcttg aagaaaaggc tgctcctctt gtccaatata ggctgcctca ggaacatctt
    11221 gaaggaatct atccaagatc cttctaacca tctctgtgaa ctcgcctctt tcagagaaat
    11281 gattttcttc catccagatc atctcccatt tttatttata cacaaggatt aatcgtcaat
    11341 aggccaagat tggtctatgt aagattccct gaaaaacgat cagtaccgaa atgcagctca
    11401 ccaattcaat ataaaagcac tagaagtgaa accaaagctg ctattgatag ctgccttgtt
    11461 tagattgggc tgagatcttt atcagaaagg tgtttgatca gaatttcaga tttggtaaat
    11521 attacagcag caatgtttac atctgctttt agaatcaagc tttggatttc tttgatgtca
    11581 tcgagggctg ccgtattgtg tggctgccaa aaatgtacca ggtaggcttc cttattcccg
    11641 ttacccttcg aataacgact attaagaagt ttcttgtgat cactctcaag atgttcaaat
    11701 ggatagttaa gaccgatttc aactacaaag tctggtttca agtatccccc agtgaagatg
    11761 tcgatttcac tgagattagt tgcgttagaa gggaagacgt cattttctct gctactctct
    11821 agagggtgcg ctaaatcctt gtcactaaga atggcaagat caacgtttcc tctccttgta
    11881 tgtccttctc ttgtaaaggg ttcaggccac tctttgtgaa ctagagatgc ctttcgacca
    11941 ctacttccaa taggatacaa accctgtaaa gctctgtgag aagcaagcat tgcatagagt
    12001 tcacagtgaa gagaatgttc acaacgatgg atatatggct ctctcacgaa attgtcgaca
    12061 agactgtcga tggaagtctc aaggattttc tttacgcctt caaaagaata catgaaacca
    12121 cctccagtta ttattagaaa tcatttgacc gttcacttca ctacgttatt agcataaaga
    12181 gctgcactat atatagtgcg cacggcttgt ctgaagaaag acacaggaag tagttagtgc
    12241 attcatacga ttagtatata acatattgct ttctatgcca aataaaacat ttcgatcaac
    12301 ataattcatt tttgaattat tttgctctta gaacttgcac caagatggtc caggcacaga
    12361 tcaaaaatct cttaacgcct agtatctaag cactaaagcg ttttgcagat acggaaccct
    12421 atgaagcccc ctctgtgagt agggttacag taactgcgac tagcaactgt cgtctcactt
    12481 gcgtgcgact gataactgcc gccacgaatg acccgattag aactgtttgt gctgttgtat
    12541 ggattggtct ttggcgaaca ggaatatgaa ctatgaaagt cactacacca ctcttccaca
    12601 ttaccggaca tatcatacaa tcccagctcg ttaggcgctt tcttcccgac tttttgaatc
    12661 ttacacttgt tacctataaa cttgtctata tcattctcat caagatactc atctcctgaa
    12721 ttccgccggc accaagacac gtcatctacg ttgtcgctac cggaatattt atagcccctg
    12781 ctcttgttcc cgccacgggc ggcatattcc cattcggctt cagtaggcaa cctgtatcct
    12841 actaccttcg acggatctgt agttactctt ccgtctttat caaggaagtt tccttcgctg
    12901 tcatacgctt tgagaagccc ttccatctcg ctcagccagt tgcagtactc aatggcgtca
    12961 aaccattcca cataggttgc aggtctgatt cctcctcccc aaccgtcatc gctgttttta
    13021 cttctaccgg tagcctcaca gaaagcgttg tattcattga aagtaacttc atacttgcca
    13081 atatagaaat cgtaggtaag agtcaccttg tgggtcggtt tttcattgct tttcccgtct
    13141 ccccacgtgt tgcccattgt gaagctgccc ttctctacca gggcgaaatg gttcttcgta
    13201 gccacggcgg atacgtgtcc ttctttgaag aaaattctgt ctattctatc ctgatatacc
    13261 tctatcaccg atcccgcgtc cagctctatc tgtatgtctt ctatctccag tattccatgt
    13321 acgctgtctc cgttagtcat tttcactatc gttagtagtg gattcgattc tcctatcagc
    13381 gttatggatt cagtcgtcgc aaagtcaagc ggtatctttg cgtaaattgt ggatagtatg
    13441 atgtccttcg tcagtatctg tccgtggaaa tagtctccgt tcttgagcca gataaaaggc
    13501 gtccacttca tctctcccat atcctgcgac ctctgcctta agactatctt cagtactttc
    13561 tcacgcctta tctccagctg cggcccgttt gtaagcttga agcttatgaa ggagtcgtct
    13621 atgaaccccg agaagcggtt gttgttctcg gcgattatag actctatgtt gttagcccca
    13681 ccttcgaggt tcatcccggc tatgtgtttc gtcaccacat ccaagtgagc ataggatgtc
    13741 gttatagaga atttctcgtt cacgaccgtg cccgtcagct tgtctccgtt tctgagcagc
    13801 agtatgtctg catctttttg cctctctgtg tctaggactg tctccattgc aggcctaacc
    13861 ggttctacgt tcgccacagc atctaaacta ttagcagcag aaaccagatt gctagcagta
    13921 tctttttcct tcattccttc acctcctggt ttagtgagta agaacaacgc tatttaactg
    13981 ttaccatttc ccttctcctc acgtttgcgt gccaatagct cttgtcgctt gaagccgcaa
    14041 caaaagggaa aatgcttacg agcaatcaaa gtgctgttct gcatatgcga aagcctacgg
    14101 cgttgcccgc tgaagagggc tccagat
//
LOCUS       DAOQYI010000038        13873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_4891_length_13873_cov_73.434723, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000038 DAOQYI010000000
VERSION     DAOQYI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13873
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4891_length_13873_cov_73.434723"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1110..2138)
                     /locus_tag="PKL93_08710"
     CDS             complement(1110..2138)
                     /locus_tag="PKL93_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNQ71377.1"
                     /translation="MKAAFVKSPFKFEIREVDLPPLKPNDVLLKIKASGICGTDLHTA
                     RTEAKDWQTFGHEIVGIVEEIGSCVENVKVGQSVLVESGSFCGTCENCRNGRVDLCHD
                     RAPNVFIRAEKENLTMGFAEKVIIDKQNAVPFEGVPFEEASLVEPMGVALDLTYTVDP
                     RLNDDVLVVGLGPIGLMAVQIVKAMGARRIFVANHSRSKIRIDVAKSFGVEDVILVDK
                     TPISSYKFPKGGVDRALVTAPPYVIPEVLNVMNFGGVVGFIGIDYGEGRFIKFDANKF
                     HFNKLQLRASHAVPALYFPRCIDMIKSGAVNVKPLITDTFKLEDIGEMMVRAENEKDK
                     VIKAVMLD"
     gene            complement(2150..3136)
                     /locus_tag="PKL93_08715"
     CDS             complement(2150..3136)
                     /locus_tag="PKL93_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ71378.1"
                     /translation="MKYKKVEKIDEKLSALGYGCWGISGPSFWDGTTDEDSIKTIQRA
                     IAGGINFFDVAPVYGLGHAEEVLGKAVKGLRDKIFIATKCGLLWDDQGRTRNCLKPES
                     IRKEIEDSLRRLGTDHVDLYQLHWPDPEVEIEETMETMVELKKEGKIRYIGLSNFSIP
                     EAKRAMKVGEVCSMQGLYNMLERNPKSYHNIPLDYRVEDEVLPFVLENGMAFLPYSPL
                     FQGLLAGAISDKKKFSEHDVRAANPKLNSPEFKKYYEVVVKLNRLAHEIGKPLSHIAI
                     NWLIRNEAVTSVIAGAQKVEQIEQNLGAVEWDMDDGLYERIEEIVSSSNLEL"
     gene            complement(3151..3711)
                     /gene="hxlB"
                     /locus_tag="PKL93_08720"
     CDS             complement(3151..3711)
                     /gene="hxlB"
                     /locus_tag="PKL93_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HNQ71379.1"
                     /translation="MKFEGTLDLVIDEIRSVLSGVTDESIEELSGSILNARRVFLFAM
                     GRSGLAIKAFAMRLMHLGLKVHVVGEVTSPSLGEGDLLIIGSASGETPSVVLNCKKAR
                     KFGAGIASITASKESTVAGISDIVITIPTKTPKVPDRAGVSSVQPMGNLFEQSLLILT
                     DIIVMNLMERLSIDSETMFKNHANLE"
     gene            complement(3708..4361)
                     /locus_tag="PKL93_08725"
     CDS             complement(3708..4361)
                     /locus_tag="PKL93_08725"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNQ71380.1"
                     /translation="MIRLQLANDTHIKDSFLKTAKEVADYIDILEVGTPAVLAHGMTL
                     VREISEILPDHEILADMKIVDGGYLEAAMAFESGADIVTVLGFASFKTISEVRKAADQ
                     FGGELMLDTIEISDLQSFAEKVSPLSPDYICIHTSADLSGVGESMHLLEYAKKIEIIR
                     RVLPEAKIAVAGGISPDNLSHVFPLKPDVVIAGRSIWEAEDPARVAFQIKTRLEKGS"
     gene            complement(4361..4762)
                     /gene="rbsD"
                     /locus_tag="PKL93_08730"
     CDS             complement(4361..4762)
                     /gene="rbsD"
                     /locus_tag="PKL93_08730"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF016885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HNQ71381.1"
                     /translation="MKKSGILNKEICNLIGSMGHTDLLVISDSGLPIASDRYRIDVSI
                     VGGKPGVFDVLDPVLEELEVEKVIFSEEMKTVSPKCLEETLKHLPEGIEVEFVPHVKF
                     KELTNTQSKGVIRTGEQIPYSSIILVGGVTY"
     gene            complement(4777..5526)
                     /locus_tag="PKL93_08735"
     CDS             complement(4777..5526)
                     /locus_tag="PKL93_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ71382.1"
                     /translation="MLFNADRRIALDRASAVPLYYQIYRIISDYMKEPDAVGRKLPTE
                     EAMMRVFDVSRATVRRALQALESSGQISRSRGRGTIITNNASQEQLTTIRSFTEQMRL
                     ENHVPITKVVEVKKVKAGPEMARILQVSEEEELLQVTRLRGNESYFPLALFISFLTSS
                     SNLTGEENFEGSLYELMRNRGTAVVEGDAIIEGRLAEGRIAKLLKVEEGAPILYYERV
                     GCTAIGEYVEFVQCWYEATHYKFRIHLTTKI"
     gene            complement(5571..7100)
                     /gene="xylB"
                     /locus_tag="PKL93_08740"
     CDS             complement(5571..7100)
                     /gene="xylB"
                     /locus_tag="PKL93_08740"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNQ71383.1"
                     /translation="MKYVIAYDLGTTGNKATLYGIDGKLLASSFSGYKTYHPGPNQVE
                     QDPLEWWESVKNTTPDLIARAGVNPEEIVAISFSGQMMGAIPVDRDGKLLRRAIIWAD
                     QRSVEQSARLEEVGNDFVYRLTGSRITPTYSGPKIAWIKDNEPEIYNKAHKFLNAKDY
                     IVSRFTGEFGTDHSDASMTLLFDIEKLKWSEKLVEVLGLDMEKLPDVTSSTNVVSEIL
                     PKVAEELGLSKKTLIVRGGGDGACACLGAGVVSPDEAYLYLGSSSWVSTCSTEPIFDE
                     KSRTFNFAYPIEGFYCPTGTMQAGGASYQWAKDALCQHEEEKAKALGLSAFTIMDDLV
                     DQTRPGAGNLIFLPYLLGERSPRWNINARGAFIGLSSTHRKADMLRAVLEGVVFNLKI
                     VLDILETKEKFDKIRLIGGGAKGRNWKQIVADIFGKTVTIPEYLEEATSMGAAIIGAV
                     GAGEMTFKEASTFVKDVQFIEYNPENHRYYERLFEVFDKAYNSLIETYEGLASLRTYS
                     D"
     gene            complement(7097..8029)
                     /locus_tag="PKL93_08745"
     CDS             complement(7097..8029)
                     /locus_tag="PKL93_08745"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNQ71384.1"
                     /translation="MNKVTVFGSYVMDLTTLSPHIPVVGETVFSGPFKMGPGGKGFNQ
                     AVAARKAGSDVKFMTKIGKDLFAPFVKDAFDRFGITKEYLLESDTAGTGVALIIVDEN
                     NGNNAIAVAPEACNELTVEDVIRHRDIFTSSDVFLTQFEANLDATYEAIKLARDSGAR
                     VLLNPAPVKEFDHSILKYVDIIMPNEIEASLMTGIPLDGMESIEAIASELKKSVDTVL
                     ITLGGKGVYCPSVNGGLVPACKVKTIDTTGAGDAFAGVFAAYLSRGESLERAIDYARA
                     GAAISTTRFGTSPSMPEREEIEALVNEITGGDEK"
     gene            complement(8048..8461)
                     /locus_tag="PKL93_08750"
     CDS             complement(8048..8461)
                     /locus_tag="PKL93_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ71385.1"
                     /translation="MKINKFFHVAVEVPDLDKALEFYIGLLGLKLVNREKLPEKKLEV
                     AFVAGEGCEIELMCYEDSKERKFAPESQSHFQHLSFVVDDIEKAMDYLKSNGIELESP
                     DPIPVFDGKVFYNTFKGPGGELLEIAEEKRPRSNH"
     gene            complement(8468..9460)
                     /locus_tag="PKL93_08755"
     CDS             complement(8468..9460)
                     /locus_tag="PKL93_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71386.1"
                     /translation="MAGRRERKTFTERNLLIILVVMWVFLAIATGGNFSSWANITNLI
                     RQSSINGVVAIGMTLIIITGGIDLSVGSIVGLSGMVFAIFTSTRGEFQFPTIVGILLA
                     LGISALIGLVNAVAVHDGKVPAFIATLGMMTLVRGVVMYISSGRMVTAVPLEYRRFAV
                     LEFLGIPALAWTWIILAAFMMLVLKYTKFGRNLYAIGSNEEAARLSGINIRLNMYSFY
                     IVAALFSAIAGLMLGTRMAAGVPTGGDGYELDAIASVVIGGASLSGGVGTVLGTALGA
                     LIIQTIRNGGNLLGVDPFIMQIVIGAIIILAVFFDQFIKSRKKGEGLKKLFKRA"
     gene            complement(9462..10985)
                     /locus_tag="PKL93_08760"
     CDS             complement(9462..10985)
                     /locus_tag="PKL93_08760"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNQ71387.1"
                     /translation="MPEEKTVIELEGINKTFPAVRALDNVSLSVKSGEVRGLVGENGA
                     GKSTLIKIITGAYTKDSGTMIFEGNEITRNSPLISKGLGIYAVYQDVMVTPDLSVAEN
                     FFLGQQPKIGPFINWKKMYKESMEFLENIGLDINVRKSIKELSIAESEMITISKALWQ
                     KPRLVIFDEPTAVLTRNETKILFQIIDELKNQGTAIIYISHNLEEVFDICDTVTVLKD
                     GATVGTYTTEELENVEKLIPLMVGRSIEEMYSKKDTEPGRELLRVENLSGERFENISF
                     HVRAGEVVGFFGLVGAGRTEIARALFGADLLKEGKIVVEGKDVSIKTPKDALMKGIGY
                     LPEDRRKQGIFLQQDIDFNINIINFDKVMTGPIINYGKAHSQAREYIDKLSIKIGSIF
                     QPVSELSGGNQQKVIIARWLCKNTDVLIFDEPTVGIDVGTKSEIYKLFGEILSSNKGI
                     ILISSYLPELMGMSDRIYVISNGRMAGEVHKKDFSEEFLLTLAMKNIVSREKVKEAV"
     gene            complement(11000..11695)
                     /gene="deoC"
                     /locus_tag="PKL93_08765"
     CDS             complement(11000..11695)
                     /gene="deoC"
                     /locus_tag="PKL93_08765"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNQ71388.1"
                     /translation="MNRDIPFNRRFDNTLLNPESKWEDIESFVDDTLEYNFRNVVVPW
                     YAIPAYVIEKVEGTEVGINVGPGGFPLGMVPTELKMREIEYYMSLGEAVTDFDIVINV
                     SAVKSRNWDLMKREFVTLSERVKKGNRVCKFIIETSRLTEEEIVKVCELIVDVPTIDF
                     VKTGTGFGPRATSYRDVELINSIVSGKKQIKVSGGVRTLEQVERFMELGATVFGSSSS
                     VSIYKEYEKKYGG"
     gene            complement(11773..12741)
                     /locus_tag="PKL93_08770"
     CDS             complement(11773..12741)
                     /locus_tag="PKL93_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71389.1"
                     /translation="MRKVLMLLVMASLLFGATLGFAVYEIAFIVKATDSDFWQYTIVG
                     AENAEHDLQGLVNVTVYGPPSESDIDKQVAILEDVVNKKPDAIVISSTSSEAPSLILD
                     EAYKQGIKIILIDNFVYDTGYNSFLATNNKVGGALAADKLVEYLKAAGKDLKGKIGLV
                     SAMAGVQVLTDRDDGFKTRLAEIAPDLEVLPTIYVDNDISRAANATEDLLIAYDDLVG
                     MFADNNHTGDGVARVIELQGLQDEVIAVAYDSDPQEIDALRNGSLKALIVQDPHGMGY
                     KGVMFAFMAINGEPLPEYFDTGVYVVEKDILDDSMWVLDPFSRKKY"
     gene            complement(12962..>13873)
                     /locus_tag="PKL93_08775"
     CDS             complement(12962..>13873)
                     /locus_tag="PKL93_08775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNQ71390.1"
                     /translation="KIRSLHDIAFKMESCTEEEEVYDLIVKAATEILSFDYYSLDIVE
                     GDYLIAKRNSNNIEVNTRVPKYEGIAGKTLKTGKTIIVDDVSKEIDAIPSSSKIKAVL
                     SVPIGDFGVFQTVTVESGSFKRDDAELAELLMLHALGALERIKKTREIQFLAFHDSLT
                     GLYNRTFFDEEVSRVNTSRNLPISVIFADVNGLKDLNDRFGHFYGDDYLKTISRTIKE
                     SCRHEDLVVRWGGDEFVVLLLRTDTEMAEEIVSRIEKNLSKIDCFAVEASVSFGVATK
                     RDMLTDFHDVLRNAELAMYSKKRELRR"
BASE COUNT         3380 a         3603 c         2959 g         3931 t
ORIGIN      
        1 accggcgttg gaggccggag gttggtagta ggaagagcaa agggactggc tggctgttga
       61 atggttctcc gttcgccggg agagccgtga agtaatagta tcgagattcc gaccaggagc
      121 tttgtcggaa tgacggaatt gggcgttttt ggaataaccg ttcttagtca aaccattatt
      181 tagagagtag agagaaggga aaagcgaaga gggcaagtaa ggttctggga tcttgaaaag
      241 ccttgaggag ctcttcaatt tccctcaaga gctcatcatt acacatgagt cagggagtta
      301 aattccatta atatgttggt tctcgtgcga atgctgtgtt tggcttttaa tgatcctgat
      361 cttaagtcct cccttgaggg aggagggcca cgtagtggcg gagggtgttg gtctttgtat
      421 gcgaaaaaac cgatgctctg aaatagtccg atgtgagtgg ttgctcgatg ttagttcttg
      481 gtctctggta ggagcataca tatggatcac agaccaaata acgtgaagta gtgaacatat
      541 cgttcaattt cgaacaggcc aatctattct aaacgttgtt caaagagcac acaccccacc
      601 gcaagcggtc ccccccgctc aagcggggat ttaagatcaa gaacatagag atccatgaaa
      661 ccgtcagtat ttactactaa tgctaactga ttctggaact gaggagcaaa atgattgagc
      721 tccgaagaag acgatgtggg tagtgatgag ctctcgagag gggtgtcgac ggctccatcg
      781 tcgacggggt gtgtgcacat caagaacgga actcattttc agtcatgacg gaattcttta
      841 ccaagaacga acaaccggtc ttcttggcgg aggaccgttg acggacaacg tggttttcat
      901 cagtgtgcag cgattcttag gatcatcgag attctgaaac aagttcagaa tgacggaatc
      961 tcagacaaga acctggacgc gcggcgtcgg aacgaagaac cggttcttct cggaggatgg
     1021 cggactgttg accgatgacg atcatccttt cacgttaggt tgatctcaaa gaaaaaggtc
     1081 cgccattgag gcgggccccg tattcattct caatctagca ttaccgcttt gattactttg
     1141 tctttctcgt tctccgccct gaccatcatc tcaccgatat cttccagttt gaaggtatcg
     1201 gtaatcagcg gtttcacatt tactgctccg ctctttatca tatctatgca tcttggaaag
     1261 tagagagccg gaactgcatg tgaggctcta agttgaagct tgttgaagtg gaacttgttt
     1321 gcatcgaact ttatgaagcg tccctccccg taatctattc cgatgaagcc gacgactcct
     1381 ccaaaattca tgacgttaag aacttcgggg atcacgtaag gcggtgcggt tacgagagct
     1441 ctatccactc cgcccttggg gaatttatag gagctgatcg gcgtcttgtc aacgagtata
     1501 acatcctcca ctccaaagct ctttgcaaca tctatcctta tcttagatct tgaatggttg
     1561 gcaacaaaga ttcttctcgc tcccatggcc ttgactatct gaacggccat gagaccaatc
     1621 ggacccagac caactaccag cacgtcgtcg ttcagcctag gatcaaccgt gtatgtgaga
     1681 tccagcgcaa ctcccattgg ctcgacgaga gaggcctcct caaaaggaac tccctcgaag
     1741 ggaactgcat tttgtttgtc tattattacc ttctcggcaa agcccatcgt gagattctct
     1801 ttctcggccc ttatgaagac gttgggcgct ctgtcatggc agagatctac tctcccgttt
     1861 ctgcagttct cgcatgtccc gcagaaagaa ccgctttcta ccagcacact ctgtccgacc
     1921 ttgacatttt cgacacatga accgatctct tccacaatgc cgactatctc gtggccgaag
     1981 gtctgccaat ctttggcttc agttcttgct gtgtggagat ctgttccaca gattcccgag
     2041 gcctttatct tcagcagtac gtcatttggt ttcaaagggg gaagatctac ctcccggatc
     2101 tcgaatttga acggcgattt tacaaacgcg gctttcaaat agacaccccc tacagctcca
     2161 gattggaact ggagactatc tcttctattc tttcatatag tccgtcatcc atatcccatt
     2221 cgacggcgcc gagattctgt tctatctgtt cgaccttctg agcgcctgca attaccgaag
     2281 ttactgcttc atttctgata agccagttga tggctatgtg actcagaggc ttccctatct
     2341 catgcgccag tctgttcagt tttacgacga cctcgtagta tttcttgaac tcaggtgaat
     2401 ttagcttcgg attagcggcc cgtacgtcat gttcgctgaa ctttttcttg tcgcttatcg
     2461 ctccagccag gaggccctgg aagagcgggc tatatggcaa aaaggccatt ccattctcca
     2521 aaacgaaggg aaggacttca tcttcgactc tgtaatcgag cggaatatta tggtagctct
     2581 ttggattcct ttcgagcata ttgtagaggc cctgcatact gcatacctct ccaaccttca
     2641 tcgctctttt cgcctcggga atggaaaaat ttgacagccc tatgtatctg atctttccct
     2701 ctttcttcag ctcgaccata gtctccattg tctcttcgat ttcgacctct ggatcgggcc
     2761 agtgaagctg atagagatcc acatgatccg tcccgagtct tctgagacta tcttcgatct
     2821 cttttcttat gctctcaggt ttgagacagt ttctcgtcct tccctgatcg tcccagagaa
     2881 gaccacactt ggttgcaatg aagatcttat ccctcaaacc ttttactgcc ttcccgagta
     2941 cctcctcggc gtgtcccaga ccatacaccg gcgcaacatc gaagaagttt attcctccgg
     3001 ctattgccct ctggatcgtc ttgatcgagt cttcatctgt cgtcccatcc cagaaagacg
     3061 ggcctgagat tccccagcag ccgtaaccga gcgccgacag cttctcatct atcttttcca
     3121 cttttttgta cttcaattcc ttcgcctccc ttattcgaga tttgcgtggt tcttgaacat
     3181 cgtttccgaa tctatgctca acctctccat taaattcatt acaatgatat ccgttaggat
     3241 caatagtgat tgctcgaaca gatttcccat cggttgaacc gaagagacgc cagccctgtc
     3301 gggcactttg ggagtctttg tgggaatcgt aatgactata tcgcttattc ccgccacagt
     3361 agattctttg ctggcagtta tggaagctat tccggctcca aattttcggg cctttttgca
     3421 gttaagaacc actgaaggag tctcccccga tgcagatccg atgattaaaa ggtctccctc
     3481 tcccaaagag gggctggtta cttctcccac aacatggacc ttcaaaccca gatgcataag
     3541 cctcatcgcg aaggccttga tcgccaggcc agacctgccc atcgcaaaga gaaagactct
     3601 tcttgcattt agaatacttc cggaaagctc ttcgatcgat tcatctgtaa ctcccgaaag
     3661 gacggatctt atctcgtcaa taacaagatc aagcgtgcct tcgaatttca tgaacctttc
     3721 tccagtctcg tctttatctg gaacgccact cttgcagggt cctcagcctc ccagatcgat
     3781 cgtcccgcga tcaccacatc gggcttcaac gggaagacat gggagagatt gtcgggagat
     3841 attccgccag caacggcgat ttttgcctct ggaagaactc tccgtataat ctctatcttt
     3901 ttcgcgtact caagaaggtg catgctctca cccacaccgc tcagatcggc cgaagtgtgg
     3961 atgcatatgt aatccggtga tagcggagag accttttcgg caaaggattg gagatccgaa
     4021 atctctattg tatccaacat aagttctccg ccaaactggt cggcagcctt tctgacttcc
     4081 gaaatcgtct tgaaggaagc gaacccaaga accgtcacga tatctgcccc gctttcaaag
     4141 gccatcgccg cttcgagata acccccatct acaatcttca tatctgcaag aatctcatga
     4201 tcgggcagga tctcgcttat ctctctcacc agagtcatac cgtgcgcaag aacggcaggc
     4261 gtaccgactt cgagaatgtc tatgtaatcg gcgacttctt tggcagtctt tagaaagcta
     4321 tcctttatgt gagtgtcgtt tgcgagttgt aaacggatca tcaataggtt accccgccaa
     4381 cgagaatgat acttgagtat ggtatctgct cgccggttct gattactccc ttagactgcg
     4441 tgttggtgag ttccttgaat ttgacatggg gaacgaactc cacttctatg ccttcaggaa
     4501 gatgcttcaa cgtctcctcg aggcatttcg gactaacggt cttcatctct tcggagaaga
     4561 taaccttctc gacttcaagc tcttccagca cgggatctag aacatcgaag acccctggct
     4621 ttccgcctac aatacttacg tcaatcctgt atctgtcaga agcaataggc agccctgagt
     4681 cagatattac cagtaagtct gtgtggccca tcgaacctat tagattgcat atctccttgt
     4741 tcagaattcc gcttttcttc ataattatct ctcccttcaa atctttgtag tcaaatgtat
     4801 tcggaatttg tagtgggtcg cttcgtacca gcactgcacg aattcaacat actcaccgat
     4861 agcagtacag cctactcttt cgtaatatag gatcggcgcc ccctcttcta ccttcagaag
     4921 cttcgctatt ctcccttctg cgagccgtcc ctctatgatc gcgtctcctt cgactacggc
     4981 cgttccccta tttctcatca gttcataaag agaaccctca aagttctctt ctccggtaag
     5041 gtttgagcta gatgtaagaa aggatatgaa gagcgcaaga ggaaagtagg attcatttcc
     5101 cctcagtctg gtaacctgaa gcaactcttc ctcttccgat acctggagaa ttctcgccat
     5161 ctcaggaccg gccttgactt tcttcacctc aacaaccttt gtaatcggca catggttctc
     5221 aagtctcatc tgttcggtaa agcttcgaat agttgtaagc tgctcctgag aagcgttgtt
     5281 cgttatgata gtcccccttc cacgagacct ggagatctgt ccgctgcttt ccagggcctg
     5341 caaagctctt cttacggttg ccctgctaac gtcaaacact ctcatcattg cttcctccgt
     5401 tggaagtttt ctaccaacgg catcaggttc cttcatgtaa tccgagatta ttctgtatat
     5461 ctggtagtag agtggaaccg cacttgctct atcaagggct attcttctgt ctgcattaaa
     5521 aagcatgatg aaatcacatc cttctttacg gtactcgaga gacaaacagg ctaatccgaa
     5581 tacgttctca gacttgcaag gccttcatag gtctctatga gcgaattgta ggccttatcg
     5641 aaaacttcga aaagtctttc gtagtacctg tgattctcag ggttgtactc gataaactga
     5701 acatccttaa caaaagtaga cgcttccttg aacgtcatct ctccggctcc aacagcacca
     5761 ataatggccg ctcccattga cgttgcctct tcgagatact cgggtatcgt aaccgtcttc
     5821 ccaaatatat ctgccacaat ttgtttccag tttcttccct ttgctcctcc accgataagg
     5881 cgaatcttgt cgaatttttc cttagtctcg agaatatcca ggactatttt caggttgaag
     5941 accacacctt cgagcaccgc cctcagcata tcggcctttc tatgtgtaga tgaaagtcct
     6001 atgaaggccc ctctagcatt aatgttccac ctcgggcttc tctccccgag caaataaggc
     6061 agaaagatga ggttcccggc tccgggtctt gtctggtcaa ccagatcgtc cataatcgtg
     6121 aaggcgctca gccccagtgc ctttgccttc tcttcttcat gctggcagag agcgtcctta
     6181 gcccattgat acgaagctcc cccggcctgc attgttccag tcgggcaata gaaaccctca
     6241 atgggatatg cgaagttaaa tgtcctagat ttctcgtcga aaataggttc cgtggaacat
     6301 gtgctcaccc agctagatga accgagatag agatatgcct cgtctggact gacaacccct
     6361 gctcccaggc aggcgcaggc gccgtctccg ccgccgcgga ctattagagt cttcttcgac
     6421 agtcccagtt cctccgccac ctttggcagt atctccgaga cgacattcgt agaggaggtc
     6481 acatcgggaa gtttttccat atccagcccg agcacttcaa cgagtttctc cgaccatttc
     6541 agcttttcga tatcgaagag aagcgtcatc gacgcatctg agtggtcagt cccgaactct
     6601 ccggtaaatc ttgagacgat gtaatccttc gcattcagga atttatgtgc tttattgtat
     6661 atttcgggtt cgttatcttt tatccaggca atcttcggac cggagtaggt cggcgttatc
     6721 ctagatccag tgagcctgta tacgaaatca ttgcccactt cttcgagcct tgccgactgt
     6781 tcgacgcttc tctgatcggc ccatattatg gcccttctaa gaagctttcc atccctgtca
     6841 acggggattg cccccatcat ctgaccgctg aagcttatag cgactatctc ttcgggattg
     6901 acaccggccc tggcgatgag atccggggtc gtattcttga cggactccca ccactccaga
     6961 ggatcctgct ctacctgatt cggaccggga tgataggtct tgtaacctga gaaggaactc
     7021 gccaggagct tcccgtctat tccatatagt gtcgccttgt tccctgtagt tcccagatcg
     7081 tatgcgatga cgtatttcat ttctcatcgc ctccagttat ctcattaacg agagcttcta
     7141 tttcttccct ttccggcatt gaaggcgaag tgccaaatct agtcgtagaa atggccgctc
     7201 cggcacgggc gtagtcgatg gctctttcca agctctctcc cctcgagaga tatgcagcaa
     7261 aaactccggc aaatgcgtct ccagctccgg ttgtatcgat agtcttcacc ttgcaggcgg
     7321 gaacgagccc tccattgacc gatggacagt aaaccccctt gcctccgaga gttatcagca
     7381 ctgtgtccac cgacttcttc aattcagaag ctatggcctc tatcgactcc attccgtcca
     7441 gcggaattcc cgtcattagg ctggcctcta tctcgttcgg cataataatg tcgacatatt
     7501 ttaggatcga atgatcaaat tccttcacgg gcgcggggtt tagcagaact ctcgctccgg
     7561 agtctctggc gagttttatc gcctcgtaag ttgcgtcgag attcgcttca aactgagtaa
     7621 gaaagacatc ggaagaggtg aagatatctc tgtgtcttat aacatcttcg acagtcaact
     7681 cgttgcaggc ctctggcgca actgcaatgg cgttgtttcc gttgttctca tctactatta
     7741 tcagagcgac tcctgttccg gcagtatccg actcaaggag atactccttt gtaattccaa
     7801 atctatcgaa tgcatccttc acgaatggag caaacaagtc cttgccgatc ttcgtcatga
     7861 acttgacgtc tgatccggcc tttctggcag ccacagcctg attgaatccc ttgcctccgg
     7921 ggcccatctt gaagggaccg gaaaagaccg tctctcctac aacgggtata tggggagaaa
     7981 gggtagtgag atccattaca tagctaccaa atactgtcac tttgttcatc tgatacctcc
     8041 aaaaagttta atgattcgat ctgggtctct tctcttccgc gatctccagc aattcaccgc
     8101 ccggaccctt gaacgtgttg tagaaaacct ttccgtcaaa aaccggaata ggatcgggcg
     8161 attcgagttc aatgccgttg ctcttcagat aatccattgc cttttcaatg tcatctacaa
     8221 cgaaagagag gtgctgaaag tgagactgtg actcgggagc aaatttcctc tccttgctat
     8281 cttcgtaaca catgagttcg atttcacaac cttcgccggc aacgaatgct acttcgagtt
     8341 tcttttcagg cagcttctct ctgttaacca gtttcaaccc gagaaggcct atatagaact
     8401 cgagcgcttt atcgagatcg ggaacttcta ctgccacatg aaagaactta tttatcttca
     8461 ttatctatca cgcccttttg aagagtttct taagaccttc gcccttcttc ctgctcttta
     8521 tgaactgatc gaagaagacc gcaaggatta ttattgctcc gataacgatc tgcattataa
     8581 atggatctac accaagaaga ttaccgccat ttcttatggt ctggattata agagcgccaa
     8641 gggccgtccc aagaacggtt cctacccctc cgctcagact tgctcctccg atgacgaccg
     8701 aggctatggc gtcgagttcg taaccgtctc ctcctgtcgg gacccctgcg gccattctcg
     8761 ttcccagcat caaaccggct atagcgctga acaatgcggc cacaatgtag aaggaataca
     8821 tgttcagccg gatatttata cctgacagcc ttgcagcttc ctcattgctt cctatggcgt
     8881 atagattcct tccgaatttc gtgtatttga gcacgagcat catgaaggcg gcgagaatta
     8941 tccacgtcca ggcgagcgcc ggtattccga gaaactccaa aactgcgaat ctcctgtact
     9001 ccagaggaac ggcagtcacc attcttccgc tggatatgta cattactaca cctcttacga
     9061 gcgtcatcat acctagggtt gcaatgaagg ccggtacctt tccatcatgt acggcaactg
     9121 cgttgaccaa acctatcagc gcactgattc ccagtgccag caatatacct acaattgtgg
     9181 gaaactgaaa ttctccccta gtagaagtga atatcgcaaa taccattccg ctgagtccaa
     9241 caatagaacc aaccgaaaga tctattccgc cagttatgat tataagggtc ataccgatgg
     9301 caaccacacc atttatggat gactgcctaa tgagattggt tatgttagcc catgaggaaa
     9361 agttgcctcc tgttgcgatg gccagaaaga cccacatcac aaccagaatg atcaaaaggt
     9421 ttctttcagt aaatgtcttc ctttctctcc ttccagccat ttcagaccgc ctcctttacc
     9481 ttttctcttg agacaatgtt cttcattgcc agtgtcagca aaaactcctc ggaaaaatct
     9541 ttcttatgaa cctcgccagc cattctaccg ttagaaataa cgtagatgcg gtcggacatt
     9601 cccattagct cgggaagata tgaagagatt aaaattattc ccttgttgct ggagagtatc
     9661 tcaccgaaga gtttgtatat ctctgatttc gtcccaacgt cgattccgac ggtcggttca
     9721 tcgaagatta gaacatctgt gtttttgcag agccatctcg cgatgattac cttctgctgg
     9781 tttccaccac tcagctcgga aaccggctgg aagatgctgc caatctttat tgagagcttg
     9841 tcgatatatt ctctggcctg tgaatgagcc ttcccatagt tgatgatagg acccgtcatc
     9901 accttgtcaa agttaattat gtttatattg aaatctatat cctgctgcaa gaagattcct
     9961 tgttttctcc tgtcttccgg aagataacca atacccttca tcagcgcatc cttcggtgtc
    10021 ttgatcgaaa cgtctttgcc ttcaaccact attttccctt ctttgagaag atcggcaccg
    10081 aaaagcgccc gtgcaatctc agttcttcca gcgcccacca ggccaaagaa tcccacgact
    10141 tcgcctgccc tgacatggaa actgatattc tcgaatctct ctccggaaag gttctcaacc
    10201 ctcaggagtt ctctgccggg ttctgtatcc ttcttcgaat acatctcctc gatcgatcta
    10261 ccaaccatta gagggatcag cttctcaacg ttttcaagct cttccgttgt gtaagttccc
    10321 accgtggccc cgtccttcaa aacggtaacc gtatcacata tgtcaaagac ctcctcaagg
    10381 ttatgcgaga tatagattat ggccgttccc tgatttttga gctcatctat tatctgaaag
    10441 aggattttag tctcgtttct tgtaagcacg gcagtcggtt catcaaagat gacgagtctg
    10501 ggcttctgcc agagtgcctt ggatattgta atcatttcac tttcagcaat gctcaactct
    10561 ttaatgctct tgcgaacgtt tatatccaga ccgatgtttt ccagaaactc cattgattct
    10621 ttgtacatct tcttccagtt tatgaatggg ccaatcttag gttgctgccc cagaaagaag
    10681 ttttctgcca ctgaaagatc gggagttacc attacatcct gataaacggc gtagattccc
    10741 aaccccttgg atatcaatgg tgagttcctg gtaatttcat ttccctcaaa gatcattgtc
    10801 cctgagtcct tcgtgtaagc acctgtgatt atctttataa gtgtagattt cccagctccg
    10861 ttctctccca caaggcctct tacctcacca ctcttgacgg agagggagac attgtcaaga
    10921 gctctaacgg cagggaaggt tttgtttata ccttctagct caattacggt cttttcctcg
    10981 ggcatgtcat cgctctcctt tagccaccgt atttcttttc atactctttg tatatggaga
    11041 cgctggaact cgaaccgaag actgtggctc ccagttccat gaatctctcg acctgttcga
    11101 gagttctgac gccgccggaa actttgatct gtttcttccc ggaaacgatg gagttaatga
    11161 gttcaacatc gcgataggag gttgctctgg gaccgaaacc cgtcccggtc ttaacgaagt
    11221 cgatcgttgg aacgtcgact atcagttcac ataccttgac tatttcctct tcagtcaacc
    11281 tgctggtctc gattatgaat ttacagactc tgtttccttt cttcactcgc tcggaaagag
    11341 ttacgaactc ccgtttcatc agatcccagt ttcttgattt gactgcggat acgtttatca
    11401 caatgtcgaa atcagtaacc gcctctccga gagacatgta gtattctatt tctctcattt
    11461 tcaactctgt gggaaccatg ccgagcggaa agcctcctgg gcctacgtta attccaacct
    11521 cagttccctc gactttttca ataacataag cagggatcgc ataccacgga acaaccacat
    11581 ttctgaaatt gtactcgagt gtatcgtcaa cgaagctctc aatatcctcc cactttgatt
    11641 cgggattcag caaagtatta tcgaaacgcc tgttaaaagg aatgtctcta ttcataaata
    11701 cctcccggga taggctaaaa tttctgaaaa agggcagctt tcgctgccca taatcgttag
    11761 aaacttaaag tatcagtact tcttccttga gaatgggtcg agaacccaca tagaatcatc
    11821 aagtatatcc ttctcgacta cgtagactcc agtatcgaaa tactcgggaa gcggttcgcc
    11881 attgattgcc atgaaggcaa acatgacacc cttgtaaccc ataccgtgag gatcctgaac
    11941 gataagagcc ttcaaggagc cgtttctgag agcatcaatt tcctgcgggt ccgagtcgta
    12001 tgcaaccgcg atgacttcat cctgaagacc ctgaagttcg ataactctgg caactccgtc
    12061 acctgtgtgg ttgttgtccg caaacattcc aactagatcg tcgtaagcga ttaagaggtc
    12121 ttccgtagcg ttggctgctc tagaaatgtc gttgtcaaca tagatcgtgg ggaggacttc
    12181 tagatcagga gctatctcgg cgagtcttgt cttgaatcca tcgtctctat ctgtaagaac
    12241 ctgaacacca gccattgcgc ttactagacc gatcttaccc ttgaggtcct tacctgctgc
    12301 cttgagatat tcaacaagct tgtcggctgc aagagcgcct ccgactttgt tgttggtggc
    12361 gaggaatgaa ttgtatccgg tgtcatagac aaagttgtca atgagaatga tcttgatacc
    12421 ctgcttgtaa gcttcgtcaa gaatcagaga aggagcttcc gagcttgtcg aagaaataac
    12481 gattgcgtct ggcttcttgt tgacaacgtc ctcaagaata gcgacctgct tgtcgatgtc
    12541 tgattcggac ggaggtccgt agaccgtgac attaacaagg ccctgtagat cgtgttcggc
    12601 gttctcggcg ccgactatcg tgtactgcca gaagtcagaa tccgttgcct tgactatgaa
    12661 ggcaatttcg taaactgcaa atcccagtgt ggctccgaaa agaagtgaag ccatgacaag
    12721 taacataagt acttttcgca ttagcaaaac cctccttata aagttgttct gtacatacga
    12781 atgtacacat gtacgtacaa acataataat tataacaagc gaacgaactc gaaacaaatt
    12841 tgaatgacag aggaaatctt agtgataaga agtttagaga aaaaaagaag acataatcgt
    12901 ctttaatcac tcttaatcat cgccagttgt ttcaggaaag gttggtcttc tggactctgt
    12961 cttatcttct gagttctctt ttctttgaat acattgcaag ctccgcattt ctcagcacat
    13021 catgaaaatc agttagcatg tctctcttag tggctactcc aaatgagaca ctagcttcca
    13081 cggcgaaaca gtctatcttg ctcagatttt tctcgattct tgaaacgatt tcctctgcca
    13141 tttcggtgtc tgtcctcaga agaaggacaa cgaactcatc tccaccccat cgaacaacta
    13201 gatcctcgtg cctgcaggat tctttgattg tgcgagatat agtcttcaga taatcgtcgc
    13261 cgtagaaatg gccgaaacgg tcgttcaaat ccttcaggcc gtttacatcc gcgaaaatta
    13321 cggaaattgg aagatttctc gaagtgttta ctctcgatac ttcctcatcg aagaaggtcc
    13381 tgttgtaaag accggtcagc gaatcgtgaa aagcaaggaa ctgtatttct ctagtcttct
    13441 tgatcctttc aagggctcca agagcatgaa gcatcaacaa ctcggcaagc tctgcatcat
    13501 cacgtttgaa gctaccggac tcaactgtga ctgtttgaaa aacgccgaaa tcgccgatgg
    13561 gaacactaag tacagccttg atcttactac ttgaaggtat tgcatcgatt tccttcgaaa
    13621 cgtcatcgac tattatcgtt ttcccggtct tcaaagtctt tccagcaatt ccctcgtact
    13681 ttggaaccct tgtattgact tcgatgttgt tcgagtttct ctttgcgata agatagtctc
    13741 cttcgacaat gtcaagactg taatagtcga agctgagaat ttcggtggcc gctttaacta
    13801 tgagatcata gacttcctct tcttctgtgc agctctccat cttgaaggca atatcgtgga
    13861 gacttcttat ctt
//
LOCUS       DAOQYI010000039        13200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_5208_length_13200_cov_76.295397, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000039 DAOQYI010000000
VERSION     DAOQYI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13200
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5208_length_13200_cov_76.295397"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1217
                     /gene="rapZ"
                     /locus_tag="PKL93_08780"
     CDS             336..1217
                     /gene="rapZ"
                     /locus_tag="PKL93_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNQ71391.1"
                     /translation="MSSNIFVVTGMSGAGKSSAIDVLEDFGFFCMDNVPPSIIEELVN
                     IIGAQEIDKSAVVIDIRTAKKFGGIDKVNQSLRSLKERGLGIVSIFLDAEDDVLYYRY
                     QKTRRAHPLQEETGLEEAIQKERSIMDPFKKNCDHVIDTSRMDMKEMKETIVSIIRGK
                     GYKLPPMRVEIESFSYNEGVPHDANLIFDVRFLPNPYYYEELIELTGMDKKVKDYLET
                     YPEIESYFSTVLKLCRMTIEGFAGSGRNFMKIAVGCTGGKHRSVYIADRLFEELKTDN
                     LRLSLDHREQKVHKDNS"
     gene            1249..2331
                     /locus_tag="PKL93_08785"
     CDS             1249..2331
                     /locus_tag="PKL93_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71392.1"
                     /translation="MRLKTFLLVLVVLLGVSILAVSPQLLEAAKKEGKVVVYSITSRI
                     SNAAEAFTEKYGIAVEAYNLKDFEMIEKVSREVGSGIVGADFVIAQDGGRVFGELIEP
                     GYLINYVPEDMKDIIPESMQNPLVLSVITKVFLYNSETFTYPPVANVWALTDPEFEGR
                     FFFKDPFKEGVNMNFLTMLTTPKVIEDLELAYERYFGKPIELTTENAGYEWMKGVLEN
                     GLVFINSDTTMAESLGIRGEGIDKVGLCTYSKVRYRETKNLALLPIMGMEPFAGFYYP
                     SYMMLTSNSAHPNAAKLFIEYLLTEEGFAPWAGSEGTYSSNPNILPYPGDNAFDVWEK
                     VLIGEDPEFLFNYRLDVEDFWNMYAY"
     gene            2390..4183
                     /locus_tag="PKL93_08790"
     CDS             2390..4183
                     /locus_tag="PKL93_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ71393.1"
                     /translation="MKKLLNKLLAYFSKPEHMILVVLLIILIYLTIVPLFYIGVDTFT
                     VHSAEVRRIRGSNVGDFTLYHWEYVFNSSASDNLFYKPLINTLLVSVATCAFALIVGG
                     FFAWMVTRTDLRFKKSISTMMLFPYIMPSWTLAMAWLNFFKNKSIGGVPGIFSALTGI
                     ETADWFAYGFIPIVLVLGMHYAPFAFILIGGILRNMDANLEEAAVILKTSRLRIIWKI
                     TVPIVMPAILSTFLLVFSSAMSAFAVPSFLGGPVRYQVLTTQLYRTINGVNPGAGYII
                     ASFMIVVGVAILTINQIIIGKRKGFTTVTGKSGNISLVNLRKLRTPVSTIMLVILVAV
                     AVIPLMTFAAQSFFVEPGNYSLSNLSTIFWTGEGSPDIANGEPGILKNKYVYQGLFNS
                     FSLSIVVALIAGTVGILVGYSVAKTRGKFGSNMVDRLAFFPYLIPSMAFGAIYLSMFS
                     VQRGFIPPLYGTFAILALVGSVKYLPFASRAGINAILQLGKEIEEAAIILGVKWWKRM
                     LKIIIPIQKPTVISGYLLPFISSMRELALYILLVTPGTSILTTMLFKYNEKGWNQYAN
                     AIVLLIIGIVLIFNFLINKVTGASLDKGIGG"
     gene            4188..5312
                     /locus_tag="PKL93_08795"
     CDS             4188..5312
                     /locus_tag="PKL93_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71394.1"
                     /translation="MPEIILKNLTKTFGSVKAVDNLDLTIEDRDFVTLLGPSGCGKTT
                     TLRMISGLETPTVGEIIIGGRVVFSSEKQINLSPDKRDVGLLFQNYALWPHMTVYQNI
                     SFGLENMKWSKEEIRKRVKEMGDLVKISDLLDRYPSELSGGQQQRVAIARTLAPNPKV
                     LLMDEPLSNLDAKLRMEMRAELKRLHREIESTVVYVTHDQLEAMTLATKVCLLEEGVL
                     QQYAPPLVIYDQPSNIFVGDFIGNPSMNFVDARVNEVDGDKATLEASEITLEMDFRSR
                     EGELKSGDEVIIGLRPEDIALNPESGANTCTIYSTLPSGMETVVRAKIRNTMFSIVAF
                     GRVDFDVDSKMEISFPSRNYLLFDKATKKKLGAGSLKFKD"
     gene            complement(5389..6861)
                     /locus_tag="PKL93_08800"
     CDS             complement(5389..6861)
                     /locus_tag="PKL93_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ71395.1"
                     /translation="MSKIAIIGSGIGGLAAGCYARMNGLESVILEKADKPGGLCTSWG
                     KGDYIFDGCIRHLAGLKPGTPLNNMWKDIGAMPSDIYFPEELVKAVDEKGNELTVFYD
                     LEKLEAEMKRISPEDSKPIEDYIRAIRRLSNTDLMEVGFWSFRDWLRNISVLIKNRKV
                     SKYSMLSFAQRFKSPVLRAFFPVIQYGWANIPLTLNLGVMAGSAGKRYGRYLGGSAEF
                     IKSVADRYTSLGGEILYNSSVERIIEDSGKAVGVRLENGTEISANYVISDAYGYDTFM
                     KMIDERYLTDRLRSMYSKPIDRMMMGLHVSFGVNMDLSDKPNAISFFLDEPIEIAGDN
                     CSIINVLNYSYDKKLAPKGKTSIKAMYDTYYSYWEELSKDQKKYEEEKAIIASRTIEA
                     IERLIPGIGEAVEVTDVATPITTERFTSNARGYGSKEDFRLRDTLKGFLNKPMTLKSL
                     KNFFVIGHSIGGTNLYGCAAMGRNTIRKICKMEHRNFVAS"
     gene            7079..7513
                     /locus_tag="PKL93_08805"
     CDS             7079..7513
                     /locus_tag="PKL93_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71396.1"
                     /translation="MKIICVFLVLILAVSMIGVDFSVGVKIHGHFEPFAAVNFDTWLL
                     GFQVKAGVIFATDSIVLVPGLYLYKDIASWRVHGGVEGLIHLGEMECLMLAGAGASYG
                     FKTDFGRVLVGGELGLPISLPGDFFVDATMKLVPTFLIQLEF"
     gene            complement(7565..8065)
                     /locus_tag="PKL93_08810"
     CDS             complement(7565..8065)
                     /locus_tag="PKL93_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71397.1"
                     /translation="MKRSIATAILVTVLAVSCLSRNPTIEAYRKDFYSINFMDVENLS
                     VKLTTEKIDISRNEKRMLNDGDILVYLTDEDRLGKMVILELDKNESGMLLFDFVTYDK
                     DGKVFTEKKDVKFNSSYVFDFDKGIFPKEIEGVKLWWHSIDDIEMYLVPWAPTKLLKY
                     PNAEMN"
     gene            complement(8089..8592)
                     /locus_tag="PKL93_08815"
     CDS             complement(8089..8592)
                     /locus_tag="PKL93_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71398.1"
                     /translation="MKKVSLVLCLLLAGSLLLGNLSLGLINVAAKTESLSNTPLSGFV
                     GVDVLGIDLRYDVGLLYLGVATPFLMFTLSEDTGVKSSLIIPGIAWYGYIGLKLDFGV
                     FYFKGDIGHTFAFGEQLQLGFSPLRLGFGMNFSPSYYIELSVDTVLQKFNETVGKIFD
                     FKIGYRF"
     gene            complement(8701..9744)
                     /locus_tag="PKL93_08820"
     CDS             complement(8701..9744)
                     /locus_tag="PKL93_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71399.1"
                     /translation="MATFIIRRLMFLPLVAFVVTLVVFAVIWSMGPDVLLRAYMTGNA
                     SKSPNAEQRIVEKYGLDRPAVVMYFKWFGNTLRGDMGYSLTAGSRVSEAILVRLPASL
                     ELLILALIPIILFGSRLGIRAAMHPDGFTDSVFGFLSIIGWATPDYVMALLILVGSFT
                     IFGWLPIGNLTTSLIKDWNTYSNSLIIDSILNLRLDILLAQLVNLIQPVLTLFIVHSA
                     YVFQISRATALEVKKKDFIRAARSRGVSERTIMLTHVRRNALIPVVTVGGELFASLFA
                     GLAFVETIFARPGIGRLLTEAAISFEVLTLSGLVLLIAAVMLLVNIVVDIIYSYLDPR
                     ILLWDNGKSERGE"
     gene            complement(10075..11007)
                     /locus_tag="PKL93_08825"
     CDS             complement(10075..11007)
                     /locus_tag="PKL93_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71400.1"
                     /translation="MERLKKYLDDVRTFLPGTNPRKADEILREIESHVLERAEREHGE
                     INDTAVAMSIKEYGSPEEVAARYYEGGPIIAPHLKNYLFMYTGILFAIHLGLHLIAFV
                     FGENGAIFDFRGTDLLSLLSQLPVTFIFDFGLVSLILYFVTQAKATAKLPHFTYFIRE
                     EKTPSMGKRIGVLIGSLVGAGITFLAFTYGPFYFGGGELTEIAIIALDSFKFALFLAF
                     GLLVIDSVSSLINIFNYSRFSKIVSNTLGLVFLFLAVSPDYKPDIASYLSLNPASIDH
                     PLLVAILFLMAIVVIVDLVFETIKFWVSRIVRTD"
     gene            complement(11010..11333)
                     /locus_tag="PKL93_08830"
     CDS             complement(11010..11333)
                     /locus_tag="PKL93_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNQ71401.1"
                     /translation="MDIGKLATEMNRGFIQLLVMVMLDEPMYGYDIVKTLGERNFSID
                     ENTLYPLLRRLEEREILSSEWRVEENKPRKYYFVSETGRQVRKELLRIWNEQEQLLKS
                     FVGEE"
BASE COUNT         3619 a         2990 c         3026 g         3565 t
ORIGIN      
        1 cattgctcta accaagaacg aagaacttgg acgcggagcg tcggaacgaa gaactcaatt
       61 ttttattcgg cgaacggaga accattcaac gtcaaccagt ttctcatgct cttacgaatc
      121 tccaaccact aacctccggc cccggccttt cttaaaactc acaacttgca tcttggaact
      181 gatcccttgc tcttccaact ccaaacctcc tacccccgac catgttcttc cccttcgaca
      241 ttgatctcta aagtagaatt cttccagatt attgtataat ttaatcattc tctctttctt
      301 cgttattatt taccatttgg gcggtgatgc tacgaatgtc ttctaacata tttgttgtga
      361 ccggcatgtc tggagccgga aagtcatcgg caatcgacgt acttgaagat ttcggattct
      421 tttgtatgga caatgttcca ccttcaataa tcgaggaact tgtaaacatt atcggtgcac
      481 aagaaatcga taagagcgcg gttgttatcg atattcgtac agccaagaag tttggcggta
      541 ttgataaagt caaccagagc ctcagatcac tcaaagaaag aggactgggt atagtttcaa
      601 tatttctcga tgcagaagat gatgtacttt actacagata ccagaagacc cgtagagccc
      661 atcccctgca ggaagagaca ggtctcgaag aagcaattca gaaggaaaga agcattatgg
      721 atccattcaa aaagaattgt gatcatgtga tagatacgtc aagaatggat atgaaagaaa
      781 tgaaggagac aatcgtctct attatcagag ggaagggtta caaactgcct ccgatgaggg
      841 ttgagataga gagtttctca tacaatgaag gggttcctca cgatgctaac ctgatcttcg
      901 atgtgagatt ccttccaaat ccctattatt atgaggagct cattgaactg acgggaatgg
      961 acaagaaagt gaaggattat ctggagactt accccgagat agaaagctac ttctcgactg
     1021 ttttgaagct ctgcaggatg acaattgaag ggttcgccgg aagcggcaga aacttcatga
     1081 agatagctgt tggctgcacg ggcggcaaac acagatctgt ctatattgca gatcgtctgt
     1141 tcgaggagct caagactgac aatttaaggc tctcccttga tcacagggag caaaaggttc
     1201 acaaagacaa ttcatgatcc atactcttaa aactctagga ggtgtcttat gagactaaaa
     1261 acgttcttgc tcgtactggt agttttgtta ggcgtcagta ttcttgcggt ttcaccgcaa
     1321 ctgctggaag cggctaagaa ggaagggaag gtcgttgtct actctataac aagcagaata
     1381 tccaacgcgg ccgaagcttt tactgagaag tacggaattg cagtcgaagc ctacaacctg
     1441 aaagacttcg agatgatcga aaaggtttcc agagaagtgg gaagcggtat agttggagcc
     1501 gatttcgtca tagctcaaga cggcggtaga gtattcggag agcttattga accgggatac
     1561 ttgatcaact acgttccaga agacatgaag gatattattc cagaatctat gcagaatcct
     1621 ctcgtgttat ccgttatcac gaaggtcttt ttgtacaatt ccgagacgtt cacttatccg
     1681 cccgtcgcaa atgtttgggc acttaccgat ccggaattcg aaggtagatt cttcttcaag
     1741 gatcctttca aggaaggagt caacatgaac ttcctgacaa tgctgacaac ccccaaagta
     1801 attgaggatc tcgagcttgc ctacgagaga tatttcggga agccgatcga gcttacgacg
     1861 gaaaatgccg gctacgagtg gatgaagggt gttcttgaga atggtttggt cttcattaac
     1921 agcgacacga ccatggccga gtctctcgga attaggggtg aaggcatcga taaggtagga
     1981 ctctgtacgt attcgaaggt gcgatacaga gagaccaaga atctcgccct tctaccgata
     2041 atgggtatgg agccctttgc aggcttctac tatcccagct acatgatgct gacgagcaac
     2101 tctgctcatc cgaatgctgc taagctcttc atagagtatc tcttgacgga agagggtttt
     2161 gcaccgtggg caggctctga aggtacttac tcgtcgaatc caaacattct tccataccca
     2221 ggagacaatg cgtttgatgt ctgggagaaa gtgctaatcg gagaggatcc tgaattcctc
     2281 ttcaattaca ggctcgacgt agaagatttc tggaatatgt acgcatattg atcacatttc
     2341 ttaggctctc ccgtttgggg gagccttagt cctttcaaag gtggttacta tgaagaaact
     2401 tttgaacaag ttgcttgcct atttctcaaa acctgaacac atgattctcg ttgttctact
     2461 tattattctt atctacctta cgattgttcc cctcttttac attggagtag atacgttcac
     2521 agtccactct gcagaagtga ggagaatcag aggctccaac gtaggagatt tcacgttgta
     2581 ccactgggaa tatgttttca acagttctgc aagcgacaac ttgttttata aacccctgat
     2641 caatacccta cttgtttcag tagctacgtg tgcatttgcg cttattgtcg gaggcttctt
     2701 cgcgtggatg gttacacgca ctgatttgag attcaagaaa tccatatcta caatgatgct
     2761 gtttccttac atcatgcctt catggactct tgccatggcc tggctcaact tcttcaagaa
     2821 caagagcata ggtggcgtac cagggatatt ttcggctctg actggaatag agaccgcaga
     2881 ttggtttgcc tacggattca ttccaatcgt tctcgtactg ggaatgcatt acgctccctt
     2941 tgcctttatt ttgattggcg ggattttaag aaacatggac gccaatctcg aagaagcagc
     3001 agttattctt aagacatcgc gcttgagaat aatatggaag ataacggtgc ccatagtgat
     3061 gcccgcaatt ctttcgacat tccttcttgt cttttcaagc gccatgagcg ccttcgccgt
     3121 cccttcattt ctgggaggtc cggtaaggta tcaggtactg acaacccagt tatacaggac
     3181 gatcaatggg gttaatcccg gcgccggata tatcatcgca tcattcatga tagttgttgg
     3241 agttgcaatc cttacaatca atcagataat catcggtaag agaaagggtt ttacgacggt
     3301 aacgggaaag agcggaaaca tctcgcttgt gaatctgaga aagctacgca ctcccgtttc
     3361 gactataatg ctcgttatac tggttgcagt ggctgtgata ccgctgatga cctttgcagc
     3421 tcagtcgttc ttcgttgaac cagggaatta cagcttaagc aatctttcga ctatcttctg
     3481 gacgggggag ggcagtccgg atatcgccaa cggagagcca ggaatattga agaacaagta
     3541 tgtgtatcag ggtttgttta acagcttttc gctttcaatt gtagtggcgc tgattgcagg
     3601 aacggttggg attctcgttg gatactctgt tgcgaagacc aggggaaagt tcggctcgaa
     3661 catggttgac agactggcct tcttccctta tttgattcca agcatggctt tcggcgcgat
     3721 ttatctgtcg atgttctctg ttcagagggg ctttattccg cccctgtacg gtaccttcgc
     3781 gattcttgcc cttgtaggtt cggttaagta tttgcccttt gcctcaagag caggaataaa
     3841 tgcgattctt cagcttggaa aggaaatcga agaggcggca attattctcg gcgtgaaatg
     3901 gtggaagaga atgctgaaga taataatccc gattcagaaa cctacggtta tctcgggtta
     3961 cctgcttcca ttcatctcca gcatgagaga attagctctc tacatactgc ttgttactcc
     4021 tggtacaagt attttgacaa ctatgctctt caaatacaac gagaagggct ggaatcaata
     4081 cgcaaatgca attgtgcttc tgataatcgg aatagtttta atatttaatt tcctcatcaa
     4141 caaagtgacc ggtgcgagcc tggataaagg aataggaggg tgacctgatg cctgagatta
     4201 ttttgaagaa tcttacaaag actttcggaa gtgtcaaagc ggttgacaat cttgatctta
     4261 cgatagagga ccgcgacttt gtgacgcttc tcggtccctc cggttgtgga aagacgacca
     4321 cactcagaat gatttccggg ctcgaaacac ctacggtcgg tgaaatcatt ataggcggta
     4381 gggttgtctt ctccagtgaa aaacagatca acctatcgcc cgacaagaga gatgtcggcc
     4441 tcttgtttca gaactatgcg ctatggcctc atatgaccgt ttatcagaac atctctttcg
     4501 gtctggaaaa catgaagtgg agcaaagaag agatcagaaa acgcgtaaag gaaatgggtg
     4561 atctcgtcaa gataagcgac cttcttgaca ggtatccttc agaactttcc ggtggtcaac
     4621 agcagagagt tgccattgcc aggacgctag ctcctaatcc aaaagtactc cttatggatg
     4681 agccgctttc gaacctggat gccaagctga gaatggaaat gagagccgag ttgaaacggc
     4741 tgcacaggga gatagaatca acggtggtct acgtcactca cgaccagctt gaagcaatga
     4801 ctctcgctac aaaggtctgt cttcttgaag agggagtcct ccagcaatat gcgccgccgc
     4861 ttgtaatata cgatcagccg tccaatatct tcgtgggaga cttcatcggt aatccttcaa
     4921 tgaatttcgt cgatgcaaga gtaaatgaag tcgatggcga taaagcgact cttgaagctt
     4981 cggaaatcac tttggagatg gatttcagaa gtagagaagg agagctcaag agcggcgatg
     5041 aggttattat tgggttgaga cccgaggata tagccttgaa ccctgaaagc ggggctaaca
     5101 cctgtactat ttactcaact ctaccttcgg gaatggaaac ggttgtaaga gctaagataa
     5161 ggaatacgat gttctctata gtcgcctttg gaagggtcga tttcgatgtt gactccaaga
     5221 tggagatttc cttcccttcc cgtaattacc ttttatttga taaagcaacg aaaaagaaac
     5281 ttggagccgg ttcgctgaag ttcaaggatt agaatcgcaa aatatgcaaa gaggaacact
     5341 tcggtgttcc tctttcttat gaagaactat gggtaataaa ggttaatctc aggaggcgac
     5401 aaaattcctg tgctccatct tacagatctt tctgatagta ttcctgccca ttgccgcaca
     5461 gccgtagaga tttgttccac ctatggagtg tcctatcaca aagaagttct tgaggctttt
     5521 cagcgtcatc ggtttgttga ggaatccttt gagggtgtct ctcaatcgaa aatcctcttt
     5581 acttccgtat ccccgggcgt ttgacgtgaa tctctctgtg gtaataggtg ttgccacatc
     5641 ggtcacttcg acggcttctc cgataccggg aatgagtctt tcaatggcct cgattgttcg
     5701 actggcgatt atcgccttct cttcctcata tttcttctga tccttcgaaa gctcttccca
     5761 gtaagaatag taggtatcat acatcgcttt aatactggtc ttccctttcg gtgccagttt
     5821 cttgtcatag ctgtagttaa gaacgttgat tatagagcag ttgtcgcctg caatttcgat
     5881 tggttcgtca aggaagaagg aaatggcgtt cggtttgtct gaaaggtcca tgttcacacc
     5941 gaaagataca tgcaaaccca tcatcatcct gtcaattggc ttcgaataca tcgagcgcag
     6001 ccgatcggtg aggtatcgtt catctatcat tttcatgaaa gtgtcataac cgtacgcgtc
     6061 tgaaattaca taattggcgg agatctccgt accattttca agtctcactc cgacagcctt
     6121 tccactatct tctatgattc tctccacgga ggagttgtaa agtatctcgc ctccaagcga
     6181 agtatatctg tccgccaccg actttatgaa ttcggccgaa ccgccgagat atcgtccata
     6241 tctcttgccc gccgatcccg ccatcactcc tagattcaga gttagtggaa tgtttgccca
     6301 cccgtactgg attaccggga agaacgctct caaaacagga ctcttgaacc tctgcgcgaa
     6361 tgaaagcatc gagtatttcg aaacctttct attcttaatc aatactgaga tattcctcaa
     6421 ccagtctcgg aagctccaga agcctacctc cataagatcg gtgttggaaa gcctgcgtat
     6481 agcccttatg tagtcttcta ttggcttaga atcctctggt gagattctct tcatttcggc
     6541 ttcaagcttt tccagatcgt agaagactgt cagttcgttg cccttttcgt ctaccgcctt
     6601 caccagttct tcgggaaagt agatatcgga aggcatcgct ccaatgtctt tccacatatt
     6661 gttaagtggg gtgcctggct taagccccgc aagatgacgt atgcagccgt caaatatata
     6721 atcacccttg ccccacgatg tacaaagtcc tcccggctta tcggcctttt caagtatcac
     6781 cgattcaaga ccattcatcc gggcataaca tcctgcagca agccccccaa ttccactacc
     6841 tataattgcg attttcgaca tttgcaccac ctcgagaatc aaaaaaaacc ggaaacccaa
     6901 ttcctctgca ttgttgaaaa gtgaacttat cgaagctccc gagcttcagg ccaattctac
     6961 cactccaatt tgaacatgct cgcttttcag cgctattggt gaatattgac tcagcgacct
     7021 ctgaaaatta tctccgtagc ttaaggtaga attggttgtt atggaggtga tcggattgat
     7081 gaagataatc tgtgtgttct tagtattgat ccttgcggtg tcgatgatcg gtgtagattt
     7141 cagtgtggga gtgaagattc atggccattt tgagcctttt gcggccgtaa attttgatac
     7201 atggcttctt ggatttcagg tgaaggcagg agtgatcttt gctacagatt cgatcgtact
     7261 agttcccggt ctctacctct acaaagacat tgcttcatgg agagttcacg gtggcgtcga
     7321 gggtttgatt catcttggag agatggaatg tctaatgctt gccggtgccg gtgctagtta
     7381 tggatttaag actgatttcg gcagagttct cgtgggtgga gaactggggc ttccaataag
     7441 cctgcctggc gacttctttg ttgacgctac catgaagctg gtgccgacat ttctcattca
     7501 gcttgaattc tagaacgaat ctactgaccc tatgaaatgt cttctcgaac taatgtcccg
     7561 ctggtcagtt catttcagca ttgggatact tcaagagctt agtaggggcc catgggacaa
     7621 gatacatttc aatatcatca atgctgtgcc accacagttt gaccccttct atctctttag
     7681 ggaatatgcc tttgtcgaaa tcaaaaacat atgaggagtt aaatttgaca tctttctttt
     7741 cagtgaagac ctttccatcc ttatcatacg tgacaaaatc aaacaagagc atgccgctct
     7801 cattcttgtc aagttccagt attaccatct ttccaagcct atcttcgtcg gtaaggtaga
     7861 caagaatatc gccatcattg agcattcgtt tctcattcct cgagatatcg attttttctg
     7921 tagttagttt tacacttagg ttttctacat ccatgaagtt gatcgagtag aaatcttttc
     7981 tgtaagcctc aatcgttgga tttctggaaa gacaagagac tgccagcaca gttaccaaaa
     8041 tggccgttgc aatagatctc ttcatctctg aacacctctt ttcatcttct agaatctgta
     8101 acctatctta aagtcaaaga tcttcccgac cgtctcgttg aatttctgca gaacagtatc
     8161 gacgcttagc tctatgtagt aactgggact gaagttcatt ccaaacccaa gtcttagagg
     8221 agaaaaaccc agctgtagct gttcgccgaa agcaaatgtg tgaccgatat cccccttaaa
     8281 atagaaaaca ccaaaatcga gtttcaatcc aatgtaacca taccatgcaa ttccgggaat
     8341 tattaatgaa gacttcactc ctgtatcttc cgaaagagtg aacatgagaa acggagtcgc
     8401 cactccaagg tacaaaagtc ctacatcata gcgcaggtca atacctagca catctactcc
     8461 tacgaagccg gaaagaggag tatttgaaag ggattcggtt ttggctgcga catttatcaa
     8521 tcctaaagaa agatttccaa gtaataagga cccggcgagc aatagacaca ggactagaga
     8581 gactttcttc atagatctca ccccctaaag ttcattgtac gtccactatg caagaaaaga
     8641 atctgatttt cagactgttt gcttcgctgc tcgggaattc tacataaagc tctaatacaa
     8701 tcattctccc cgttcgctct tgccattatc ccagagaaga attctgggat ctaaataaga
     8761 gtaaataata tcaacgacaa tattcacgag cagcattacg gcagcaataa gaagaacaag
     8821 accagagagc gtgagaactt caaagcttat tgctgcttcc gtaagcagcc gtccgatgcc
     8881 aggccttgca aaaatagtct caacaaatgc gagccctgcg aaaagcgaag cgaagagctc
     8941 tcctcccact gtaactacag gaataagcgc attccttctt acgtgagtca gcatgatggt
     9001 cctttcgctc acacctcgtg atctagcagc ccttatgaag tctttcttct tcacttccag
     9061 tgcagttgcc cggcttatct gaaagacata agcagaatgt acaatgaaca gagtcaaaac
     9121 aggttggatt agattgacta gctgagccag caggatatct agacgtagat taagtattga
     9181 gtcgattatc aaagagttgc tgtaagtgtt ccagtctttg ataagagaag tggtcaggtt
     9241 gccaatggga agccatccga atattgtgaa actgccgact agaatgagga gcgccattac
     9301 ataatcggga gttgcccatc cgattatcga aaggaatccg aatacagaat ccgtaaatcc
     9361 atcgggatgc attgcggccc ttattccaag tctcgaaccg aagagtatta tgggaatcag
     9421 ggccagtatc agtagttcga ggcttgccgg aagccttaca aggatcgctt cagagactct
     9481 tgagccggca gtaagagagt aacccatatc gcctctgaga gtgtttccga accacttgaa
     9541 gtacataact actgcaggcc tgtcaagccc gtacttttca actatcctct gctcggcgtt
     9601 tggtgatttc gaggcgttcc ccgtcatata ggctctaaga aggacatcag gccccataga
     9661 ccagatgact gcaaagacga caagcgtgac tacaaaggcg acaagcggca ggaacataag
     9721 ccttctgata atgaaagtgg ccacatcgac cctcctagtt ttggaaaaca acttatcaaa
     9781 gagttgaact gcaacttcac atcttttcag ctaaagacat tcccgataga ttagaagcgc
     9841 aattatgatt ttatcatcaa ggagagggtg ttagtatatt taaagcgcag gacagaagtc
     9901 cgttgatatc acaagtggac ttatccaaac aacctcattc tgaactggaa agacagtgac
     9961 gggaaagcct tagaaagagt gaacgtctaa tcttcatagc ctgtatgttt tgtttctaaa
    10021 aacagtagaa aaaagggaga ggtatcaccc ctcccagttt ctaaaggagt ctaatcagtc
    10081 tgttcgtact attctgctga cccagaactt tatcgtctca aagaccagat caacaattac
    10141 tacaatcgcc atcaagaata gaatagctac cagaagtggg tgatctatac ttgccgggtt
    10201 caaactcaga taggacgcaa tgtctggttt gtaatcaggt gaaacggcca ggaatagaaa
    10261 gacaaggcca agagtattcg agacgatttt gctgaaccgg gagtaattga atatgttgat
    10321 taagctggat actgaatcta tgacgagcag cccaaaagca aggaatagtg caaacttgaa
    10381 ggaatctaac gcgatgatcg cgatctccgt gagctctccg ccgccgaaat agaatggtcc
    10441 ataagtgaaa gcaagaaagg ttatgccggc cccaacaaga gagccaatta gcactcctat
    10501 tctcttgccc attgagggtg tcttttcttc tcttatgaaa tatgtaaagt ggggaagctt
    10561 tgccgtagcc tttgcctgcg tcacgaagta aagaatcaag ctgacaagtc cgaagtcaaa
    10621 tatgaaagtc actggaagct gtgagagaag gctcagtagg tccgttcccc tgaaatcaaa
    10681 gattgcgcca ttctcgccaa aaacgaaagc gataagatga agtccaagat gaattgcgaa
    10741 aagaattccc gtgtacatga agagatagtt cttcagatga ggggcgatga tcggaccgcc
    10801 ttcataatac ctcgccgcaa cctcttcagg tgaaccgtat tctttgatcg acatcgcgac
    10861 tgcagtgtcg ttaatttctc cgtgttccct ctctgctctt tccagtacat gactttcaat
    10921 ctctctaaga atctcatccg cttttcttgg attggttccc ggtaagaacg ttcgcacatc
    10981 gtcaagatac tttttcagtc tttccatttt cattcctccc caacgaagct ttttagaagt
    11041 tgttcctgct cattccaaat tctcagcaat tcctttctca cctgtcgtcc agtttccgat
    11101 acgaaatagt atttcctggg tttgttctcc tcgactctcc actcgctcga aagaatctct
    11161 ctttcctcaa gccttcttag gaggggatac aaagtgtttt cgtcgattga gaagttcctc
    11221 tctccaagtg ttttcacgat atcgtagccg tacattggtt cgtcaagcat aaccatgact
    11281 aagagttgaa taaagcctct gttcatttca gttgccagtt tgcctatgtc cattacatca
    11341 cctctatact atgttgataa tagcatatag tatgatacac agtattctaa taacatgata
    11401 gtatcatgtg ggttaagaat taatgaaata ggaggacggg aatctgcaat ctttcatgct
    11461 gaagaaggag ccgcccttgg ttcgtagccc aagaccacaa acccgtcctt ggataagacc
    11521 tgaagttagg ctagctttgg tgggacgcaa ggccgggcaa gagcatttca agaagtgatg
    11581 ctgggaagag catcccagga agtgatgccc ggaagaacat ccggggaagt gaggccgact
    11641 tcgtcgggaa gtgatgctcg ctggggcgag gaagaataaa acattggacg cagtgcgccg
    11701 gaaagcatcg tcggtcgcaa tgttgacaga agcatgtcag gacgcaaggc tgggcaagag
    11761 catttcaaga agtgatgctg ggaagagcat cccaggaagt gatgcccgga agaacatccg
    11821 gggaagtgag gccgacttcg tcgggaagtg atgctcgctg gggcgaggaa gaataaaaca
    11881 ttggacgcag tgcgccggaa agcatcgtcg gtcgcaatgt tgacagaagc atgtcaggac
    11941 gcaaggctgg gcaagagcat ttcaagaagt gatgctggga agagcatccc aggaagtgat
    12001 gcccggaaga acatccgggg aagtgaggcc gacttcgtcg ggaagtgatt ctcgctggcg
    12061 agacgaagaa taaaacatta gaagcagtgc gccggaaagc attggcgttc gcaatgcccg
    12121 ctttgcgggg aaaataccgc tgaacgctgt gaagaaccgt ttttcgttaa ccgtccaaga
    12181 tcatggaccc gtccttcgaa agagccgttg caaattgcct agagtaaaaa aacgacaaat
    12241 gccgtcatct gaaattgttt tagcatctcg gtctttcaat aagacgagtc gagaaagagc
    12301 attgtcgaga gttcactgtg cttgccgaga ggacgagatt gattatcgtt gtccgttatc
    12361 gagatcctta accagatccc gtataggaac ccttaacagg agcacttcag ggcaggctct
    12421 acgggatgac aatcttaggt gattctgtag gggcgaacgg cggttcgccc gaagaggaag
    12481 ccctctcaaa gacgcaaggc ctgtttcgcg ggcagaagag cctctttaaa gaaaccgttg
    12541 aagccgaaga ctaagttgcc cgtccacggt cctccttcct ccgagaagat tacccgaaat
    12601 gtggggacag gttttccggt gtctgtcccc cctagtgccc atcttgcatt tgctattggc
    12661 tctcacggag aaccagatcc cgaaacaaat tcgggatgac agtgttaggc gattacgaaa
    12721 acgtcacata ctgtcattct gaacttgatt cagaatctct cgttttttca ctttgaccaa
    12781 gaacgaagaa cgggtttttt tcggcgaacg gagaaccatt caacggccaa ccagtccctt
    12841 tgctctatcc aacccccaac ccgctatccc caacgacgga aagcaccaga tcccgaaaca
    12901 aattcgggat gacagtgtta ggcgattacg aaaacgtcac ataccgtcat cctcaagaaa
    12961 cccgtccttc gggaagaacg gttattcgtt cccacgctgt gcacctaggt tcctggttct
    13021 ttgtccagaa ttccgtcatg aactcgtttc agcatcccgg cgggctttcc gaaaacgtca
    13081 cataccgtca tctgaacttg attcagaatc tctcgttttt tcactttgac caagaacgaa
    13141 gaacgggttt tcggtctgac ccttgattat tggagaataa tataaggaca ttagggttta
//
LOCUS       DAOQYI010000040        12539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_5510_length_12539_cov_72.622236, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000040 DAOQYI010000000
VERSION     DAOQYI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 12539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12539
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5510_length_12539_cov_72.622236"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKL93_08835"
     CDS             <1..197
                     /locus_tag="PKL93_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71402.1"
                     /translation="LLAQAIQKARIIAASEGLAFASTTSRRKSISKCKTHGRRRVPFS
                     KAVSKSTHPTASGPPARAGI"
     gene            986..2041
                     /locus_tag="PKL93_08840"
     CDS             986..2041
                     /locus_tag="PKL93_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNQ71403.1"
                     /translation="MLKRGFLLGVFVVLSALMLAGIVGAVAEIDKYGNVHTNIPMEAV
                     VYAAIEAGDLVYVTMGTTTIMVPFVTTYGDVDRGKPLARYSDDHVLLAINYGNFAKTY
                     GLEVGSPVYMGLVEKGAYKDELEIRHLVRSDVREDYASDAIFANFREITLGDIAPGKL
                     YRCSHPSIDDPRAPYASALIEQAGIKTVINLSDSDEELASNLEYSEYYKSISEAGNLI
                     NLNMGVDPLADDFAKKLGEGLRFMIDHEPPYLIHCVEGKDRAGIASALIGAIMNAKTD
                     EIYADYVKSYENYYNVVPGTAAYEAVRKIIVDLFVMMNDGNPVDDLNVKSVALDYLTK
                     KAGLSYTEIAAIQDILR"
     gene            2117..2686
                     /locus_tag="PKL93_08845"
     CDS             2117..2686
                     /locus_tag="PKL93_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNQ71404.1"
                     /translation="MWDALNQIELSVMDSLNAAMSGQFMDLFLSFMLFLERGALVWIL
                     LTVFLLLDKKYRRAGYMTSLGLGMSLLIAFVILKPVFGRLRPLEFIEGYTIIFPIPET
                     YSFPSSPVAVAFAYTAVLWNSVTMLRVPLALLSSLIALAEVYVYFSYFSDVIAGALIG
                     IACGIITVWLFDKYTIIKPERNDKSGGLE"
     gene            complement(2707..3495)
                     /locus_tag="PKL93_08850"
     CDS             complement(2707..3495)
                     /locus_tag="PKL93_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71405.1"
                     /translation="MRRFFVPLMLVVLVFLLSGCIWGDKPLPKIVLIDDSHANRFGGE
                     ESEFFDGIIAILAAKGYDYRYSSVVGFEPQNYAIVILSMPGASYSISDIDKLETQLTK
                     KGKIIMMDMIVEVPPASTFFLDALSDGLNTGIGFVWGLVTDTVNNIGGNKMMITSDNF
                     TTHTLASPLSAKIAMMMSSKLTVEGNASVAVFAELSAIFTTYVSSGDEVTYVPQVSEP
                     IPIVAASNIGGGKVVAISSAYAFTGTLMGLVPGNADFFRAVVDW"
     gene            4010..4819
                     /locus_tag="PKL93_08855"
     CDS             4010..4819
                     /locus_tag="PKL93_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HNQ71406.1"
                     /translation="MQRVSDDFGKNLSVSASAAMEYESQGEKMLKRVNELMAAREDIS
                     ELLGMNPLYIMYDNNSNHLQFISNVIKLNDYDLLAKSLPWVYKTYTSRDFSVEFFPEV
                     LKAWMKAIDEFLTPESAAQVIEVYREMLDSHNSAVEHAQEGLIQMKVDEDWKAIERQT
                     VLALLTGDYKKLREIANREINSEKLLADFYLKVVQPAMYEIGYLWQTGEISVAEEHLA
                     TSLMNRLMASTYSMIETVPSHPRRKVVVLSSQNEYHELGARILADLLELSG"
     gene            4835..5116
                     /locus_tag="PKL93_08860"
                     /pseudo
     CDS             4835..5116
                     /locus_tag="PKL93_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
     gene            5109..6044
                     /locus_tag="PKL93_08865"
     CDS             5109..6044
                     /locus_tag="PKL93_08865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ71407.1"
                     /translation="MNELRKVILSNIDDFFSALENLSGAILILFDNEGKILDHSPSLI
                     SLVEKSDTLKGENISDLVVDNSQALLKGHGSKPVRIGLSDSRSNIHLVSGFVMLVDES
                     RRLFLGVKIGLSAGDIMESMSKVTDELASLTRELARKNKELQAANKEIERLMNCDPLT
                     GLSNRREFMNKLDKEIESLCSTCKPLSIIMADIDDFKEVNDTRGHPEGDRVLQLFAEI
                     LNEIISPRRTTVRYGGEEFIVMLPETTSEEATEIAEEVRKNFERETEKKLGFTVTASF
                     GVATFKPSDTKSSILKRVDDALYSAKSGGKNRVVR"
     gene            7108..8256
                     /locus_tag="PKL93_08870"
     CDS             7108..8256
                     /locus_tag="PKL93_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNQ71408.1"
                     /translation="MRPDDFSSNSPGEVKKIVGPSFTYWAFVPNPLPPKLVYDNDLLF
                     LLGEANRAMGEFSGFLSGSPGTRLLIRPLFRREAVASSQIEGTTSDIEDLYFFEADQN
                     TVLLAEDVREVSNYLKALEYGLERLESLPISTRLFKELHSVLMPGVRGGYSYPGELRR
                     TQNWIGKPGCTLNEADYVPPPPEELNDCVGELENFINSESRLPPLVRLALVHYQFEAI
                     HPFVDGNGRVGRLLISLLKTYWGLMDQPGLYLSGYFEANRQEYYSRLRSVSFEGDWNG
                     WIFFFLKAVLQQSELGKNKLKEIMDLRSNWIGRLKPVSSPLPLALIDSLFENPVITIP
                     QAAERLNVTYVSARRAITALCEKGVLSQTDQSKYGKKYIASELIEIVK"
     gene            complement(8249..8419)
                     /locus_tag="PKL93_08875"
     CDS             complement(8249..8419)
                     /locus_tag="PKL93_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71409.1"
                     /translation="MCYQLSTFPEINTDFISIVETNDSEDGEPLTVDLRMLPAKGSIS
                     NTLDIPGGRVFI"
     gene            complement(8528..9487)
                     /locus_tag="PKL93_08880"
     CDS             complement(8528..9487)
                     /locus_tag="PKL93_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71410.1"
                     /translation="MYEIIDWLLEGPSWIEYRTRLDLLSQDEEEEDVVESRMKMLNLS
                     EIKSLIAELQDWPGSVLTSHKNAGHLIHKLTFLADLGIRKNDPGVEEIIEKILEHRSS
                     QGPFEVLMNIPVKFGGLGTDQFAWALCDAPVVLYSLARMGLQEDARVLGAAEYLAKLI
                     RDNGWPCVASPELGSFRGPGRREDPCPYATLVMTKLLLEIPEFLGSTEVELGVESLLS
                     LWERRRDYHPYIFYMGTDFCKLKSPEIWFDILHVLDVVSRSSLKSDDRRFSEMKDIIA
                     TKQTPSGRFIPESIWMAWKGWEFGQKKEPSRWLTLQALKILKR"
     gene            9713..9895
                     /locus_tag="PKL93_08885"
     CDS             9713..9895
                     /locus_tag="PKL93_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71411.1"
                     /translation="MSRTVLMLAGVVVLVMALLASFNVGGIVIPAWYVSLKWIGGAIM
                     ILVAYMDKGKKAKSDS"
     gene            complement(10128..12209)
                     /locus_tag="PKL93_08890"
     CDS             complement(10128..12209)
                     /locus_tag="PKL93_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNQ71412.1"
                     /translation="MLLTDHVDIPTELIRAQEEERLVVFAGAGVSMASPSNIPGFVDL
                     AKAVAKSYGQKYYVKQQRVQPPDVFLGSLNSKNGGQGVHEIVKQIMSRETSRPSQLHN
                     LIPLLFKSARDLKIVTTNYDAHFLTVLKNYGERYDGFDIFRAPALPRGDDFRGIVYLH
                     GNVEQDSRWLVVTNRDFGRAYLTEGWARDFLRDLYLSDYTILFIGYSHRDTIVDYLAR
                     GLDIRQSNRYALVSASDAERWKEINVIPVSYPLTPEKKKVHYELQKALENWTSLASSD
                     LEIHTDRIKSIAGKKPGIDQKVDSYLTYCLNREDLTEVFCRFAEGFAWVEWLDSKAML
                     DNLFREEEPLTEIEKSLTRWFVKTAFVSSSQEHLTFLASRRSSLNPYLVSSIVEQMAD
                     SDLEIPDTCQAEWLNLILNNWHKGETPCLAMLKALKKVDVTTNPWIITKAIQSFFSLS
                     QTGKPAKLSLFHRQYDLEPLVKTDCLKKFWSEIIEPNLDILHEDILSILTSQLMKAVT
                     DMSVINGSVREYDSICSARKTIEESEPESSPEWLHIYIDFVRDTILHLIKTDNKDRNF
                     YYEWWRRSRAPILWRILLYAVSEDPSCSDDDRVRIVLDNGWLINPVLKAETAKIISNS
                     LRTTSTSVLMKVVETIERELDRLSRVPNLGDGQRSNPAIGEILLAIEKSGVDSPEIKK
                     LVKPLKEKERS"
BASE COUNT         3261 a         2826 c         3052 g         3400 t
ORIGIN      
        1 gtctgcttgc gcaggccatt caaaaggcga gaatcatcgc cgccagtgaa ggcttagcct
       61 tcgccagtac tacttcgcgt agaaagagta tttcaaaatg taagacacat ggccgacgcc
      121 gagtaccatt tagcaaagcg gtttcaaaga gcacacaccc caccgcaagc ggtccccccg
      181 ctcgagcggg gatttaggag caggacttca agaggatcag cttaacgctg atgaaaacca
      241 ggttgtcagt caacggtctt ccgagaagag cctgtccttc gataagctaa aaaaaactga
      301 agagttcaga ggtatgaggg tagacgtatg aagatcaaga tcaaacatca catgccacat
      361 catgaacaat tttcagcata ttgcgttccg tagctctttt cgaacctgca accacctacc
      421 tgcagccccg atcggatcaa aaccagatcc cgaaacaagt tcgggatgac cctgaacaga
      481 agcatttcag ggcaggctca gtgaggggaa aactgacggt tatagcgaga aggctatatt
      541 tcgtcattcc gacaaagctc ctggtcggaa tcttgatgct attgatcaag gctcctctcg
      601 gcgaacggag aaccgtccaa cggtgatccg gttttcagtc gctctttcca acccccaacc
      661 cgctaccacc ggccacggtc ttcaaaagtg cagatcccga aacaagttcg ggatgacaga
      721 gtgaggggct tccacgaacc gctgcccgga taacttcctg ccgttgttat ccccacactt
      781 tgatctttcg tttctctacg gctctatctg tggtataaac tgtatatcag gtgttttaac
      841 gcggagcaac agtgccatgc ggctgtcgca ttaccttcaa atcgatcgga tagtgaaggt
      901 tgttcttttc gtgattaaaa gtcgctggtg atataattgt ggtgcaaata ccttaacgtc
      961 taaaaaagat aggtggaggt gttttatgct taaaagaggg tttttactgg gtgtatttgt
     1021 ggttctgtca gctttgatgc tagccggaat cgttggtgca gtagctgaaa ttgataagta
     1081 cggtaacgtt catactaata tcccgatgga agcagttgtt tacgccgcta ttgaagcggg
     1141 agatctggtt tacgtaacga tgggaacgac cactatcatg gttccgttcg tgaccacata
     1201 cggtgatgtc gacagaggga agccgcttgc gagatattcc gacgatcatg ttcttcttgc
     1261 gatcaactat ggcaattttg cgaagactta tggactggaa gttggaagtc ccgtctatat
     1321 gggtctagtc gagaaggggg cttacaagga tgagctggag ataagacatc ttgtgagaag
     1381 cgacgtaagg gaagattacg cgagcgatgc gattttcgcg aacttcagag agattaccct
     1441 cggagatatt gctccaggta agctttacag atgctcccat ccttcgatag acgatcccag
     1501 ggctccgtac gcatcggctc tcatcgagca ggccggcatc aagactgtca tcaatctctc
     1561 cgactcggat gaagagctcg catcaaatct tgaatactcg gagtactaca agtccatcag
     1621 cgaggccggc aacctaatca atttgaatat gggtgtcgat cctctcgcag acgactttgc
     1681 gaagaagctc ggagaaggcc ttagattcat gatagatcac gagcctccgt acctgattca
     1741 ctgtgtcgaa ggcaaggata gggccggaat cgcatctgct cttattggcg caatcatgaa
     1801 cgccaagacc gacgagatct atgccgatta tgtcaagtct tacgagaact actataatgt
     1861 agtaccggga accgcagctt acgaagcagt aagaaagatc atcgttgatc tcttcgttat
     1921 gatgaacgac ggcaatcccg tcgacgatct caacgtgaag agcgttgctt tagactacct
     1981 gacgaagaag gccggtctct cttacaccga gatcgctgca attcaggata ttctcagata
     2041 gttttataga gagtgttatt aacgaggggc ggtctagaac cgcccctttt cttagagatt
     2101 atggtggtgt tctagaatgt gggatgcgct taatcagatt gagctttcgg ttatggattc
     2161 gcttaatgca gctatgtctg gacagtttat ggatctcttt ctctcgttca tgctgtttct
     2221 cgagagggga gccttagtat ggatcctgct tacggtcttt ctcctcctag ataagaaata
     2281 ccgcagggca ggttatatga cttcgctggg actcggtatg agtttactga ttgccttcgt
     2341 tatccttaag cccgttttcg ggaggttgcg gccgctcgag tttatcgagg ggtatactat
     2401 catctttcca attcccgaga cttactcctt cccctcttct ccggttgccg tcgcttttgc
     2461 ctacactgca gtcttatgga acagcgtcac gatgctcagg gtcccgctag ctctactttc
     2521 ttccctaata gctctggcag aggtttacgt ctatttcagc tatttctcgg atgttattgc
     2581 cggtgccctt attggcatcg catgcggaat catcaccgtc tggctcttcg acaagtacac
     2641 tattatcaag cctgagagaa acgacaagtc cggcggctta gagtaatcac gcttctaata
     2701 agcgttttac caatcgacca cggccctaaa gaagtcggcg ttgcctggaa ctaatcccat
     2761 cagggttcct gtaaatgcat acgcgctgga aatcgctact actttccctc caccgatgtt
     2821 ggatgctgca acaatgggta tgggttcaga aacttgcggg acataggtca cttcgtcgcc
     2881 actggataca taggttgtga agattgcaga caattccgca aagaccgcga ccgaggcatt
     2941 cccttccaca gtcagcttgc tagacatcat catggcgatc ttcgcagaga ggggagatgc
     3001 aagcgtgtgg gtcgtaaagt tatcggatgt tatcatcatc ttgtttccgc cgatattgtt
     3061 gacggtatcc gttacaagcc cccatacaaa acctattccg gtattgaggc cgtcggaaag
     3121 agcatctaga aagaaggtgc ttgccggagg aacctcaaca atcatatcca tcataattat
     3181 cttgcctttt ttagttagct gtgtttccag tttgtcgatg tctgaaatag agtaacttgc
     3241 gccgggcatc gaaaggatca cgatcgcata gttctgcggt tcaaacccta ccacagagga
     3301 gtatctgtag tcgtaacctt tggcagcgag aattgcaatt attccatcga aaaattcgct
     3361 ctcttcccct ccaaatctgt ttgcatggga gtcatctata agaacaatct ttggaagagg
     3421 cttatctccc catatgcatc ccgaaagaag aaagaccaga acaactagca tgagcggtac
     3481 gaagaaacgt ctcattttct cacctcccat cgagatcata cgactgaagt cgaaagtaac
     3541 attatacgtg ttgaagtata gggaattaag gtggtgagac ggtaggctat ttatggtcta
     3601 agagatttcg gaatcgagtc taaagaaact tcattcagac gggaatgtgt acttggaagt
     3661 gctttgtgct tttcattcag atactgaaat aaatcggaca cttaaatgaa ttccttctgg
     3721 gaatgctccg gcatcatcag gctacctttg aaggctgtgt atcaacccga agatgtggac
     3781 tggccgtcga cacttgcggt caaatgtcgt ttctagcgtt cccggaaggg ccgaaagagg
     3841 ctagacagtt ttgcagatgg agatcaataa gtattttggc ttttatctac atcggatcga
     3901 ccggggttag atgataggct caagaattcc tgtgaactta gtctcattag tttataatgt
     3961 gtatgcggaa taggcttgaa ttcgattaaa tcaccagggg agtgtttata tgcagagagt
     4021 atcggatgat tttggaaaaa atctgagtgt ttcagcctcg gcggctatgg agtacgagtc
     4081 tcagggagag aagatgctta agagggttaa cgagctcatg gcggcgagag aggacatttc
     4141 tgaacttctc ggtatgaatc cgctatacat aatgtatgac aacaactcaa atcatctcca
     4201 attcatctca aacgtcatta agctgaacga ttacgatctc ttggcgaagt ctctaccctg
     4261 ggtatataag acttacacct cgagggattt ctctgttgag ttctttcctg aagtcttgaa
     4321 agcctggatg aaggcgatag atgagtttct aactccggaa agcgccgctc aagtaatcga
     4381 agtatatcgt gaaatgctgg attcacataa ttcggctgta gaacatgctc aagagggtct
     4441 gatccagatg aaggttgacg aggactggaa ggccatcgag aggcaaacgg tcttagccct
     4501 gctcacaggc gattataaaa agcttagaga gattgctaac cgtgaaatca atagcgaaaa
     4561 attgctcgcc gatttctatt tgaaggtagt tcagcctgca atgtatgaga taggttacct
     4621 gtggcagacc ggtgagatat ctgttgccga ggaacatctc gcgacttctc ttatgaatag
     4681 gcttatggca agcacataca gcatgatcga gaccgtacct tcgcacccca gaagaaaggt
     4741 cgtcgtgctc agttcgcaga acgaatacca cgagcttgga gcaagaatac ttgccgatct
     4801 tcttgagtta agcggataga atgtcagcta tctcggagca aatacaccgg tgtccgaagt
     4861 gctcaagcat ctgaagaaaa atctgccttt tctactggcg atctccgtta cgatgtcctt
     4921 caatctcgat ctcacgaaaa agctaattca gtccgttcac aatgaaagtg acttcgaaac
     4981 aatgaggatc atggtcggag gtctggtaat caacgagtca gaggatcttt ggaagagact
     5041 gggtgctgat ggaactgccg aatcggccgt tgaggctatc gagcttgcca aacaatggtg
     5101 gaaagagcgt gaatgaacta agaaaggtaa tcctttcaaa tatagatgat ttcttttctg
     5161 ctcttgagaa tctatctgga gcaatcctta ttctctttga caacgaaggc aagatcctcg
     5221 atcacagccc ctctctgatt tctttagtgg agaaaagtga tactctcaaa ggcgagaata
     5281 tatccgatct tgtggtagac aattcccagg ctctattgaa gggacatgga tcgaagcctg
     5341 taagaatcgg tcttagcgat tcaagatcga acattcatct agtgagcgga tttgtcatgc
     5401 ttgtcgacga gtcaaggagg ctgtttctgg gcgtgaagat cggcctttcg gccggagaca
     5461 ttatggaaag tatgtcgaag gttacggatg agctcgcatc gcttacgaga gagctagcgc
     5521 gtaagaacaa agagcttcag gctgcaaata aagagataga aaggctgatg aactgcgacc
     5581 cgctgactgg attgtcgaac agacgtgagt tcatgaacaa gctagataaa gagatagaga
     5641 gcttatgctc gacctgcaag cctctttcca taataatggc agacatagac gacttcaagg
     5701 aagtcaacga cactagaggc catcccgaag gcgacagagt tctacagctc ttcgccgaga
     5761 tcctaaacga aataatctca ccaagacgga ctaccgtgag atacggtggc gaggaattca
     5821 ttgtaatgct tcccgaaacg acgtcagaag aggctaccga gatcgcagaa gaggttcgga
     5881 agaacttcga gagagagact gagaaaaagc tcggattcac agtcacggcc agcttcgggg
     5941 tcgccacatt taagccttca gacacgaagt caagcatact aaaacgtgtc gacgacgctc
     6001 tctatagtgc aaaatccggt ggaaaaaaca gagtggtgcg ttgaaagcgg gcacatctgc
     6061 tgacgcaggc cagttcacat tgaaagaaca cgcaaggcgc cgaaaaacat cggcggacgc
     6121 attgccggcg aagaacatgc cgggacgtta ggccgactcc gtcgggaagt gatgcccgga
     6181 ggatcatccg gggaagtgag gctgcttcgc aggaggcaaa aaaccagtct gctgcgcagg
     6241 ccagtcaaaa ggcgagaatc atcgctgcca gtaccacttc gcgggagaag agccgctgca
     6301 cgcttaaaag tggagaaccc gctgatcgct gtgaagagcc ggttcttcga taagatcaaa
     6361 aaactaaaga ggtcagaggt atgagggtag acgtatgaag attaagatca aacatcttgt
     6421 gttccgtagc tcttttcgaa cctgcaacca cctacctgca accccgatcg gttcaaaacc
     6481 agatcccgaa acaagttcgg gatgacagag tgaggggctt ccatgaatcg ctgcccagac
     6541 agcttcctca gttgcagcaa aaccttgaac tggtgatccg gttttgttca cgaaacccca
     6601 gactcgaaac ccgagacccg ttttgttagg tctttttact tacaacttgg aacttgaatc
     6661 ttgcaactga ccctttgctc tttccacccc ccgacctccg acaccccaag cacggttctt
     6721 gaaaaaccca gatcccgaaa caagttcggg atgacagagt gaggggcttc catgaaccgc
     6781 tgcccagaca gcttcctcag ttgcagcaag accttgaatt ggtgagccgg ttctcttcgc
     6841 gagaccccga ccctgtaatc gaaaccccgt acttggcgtc atgttgaact tgtttcagca
     6901 tctactatct tctgcagaaa aacgaaagag agtcttgtag atagtttttc gtgctcgtga
     6961 gagaaggaaa acagagacca tagcgcatat tttttcttta aatgcctttg cttaggcaac
     7021 ttccccgatc aagtaaatta tactgataag caatatagta taattgtttg ggaaagaatt
     7081 atactcgaac ttgatttgga gggcaaaatg agaccggatg atttttcctc taattctcct
     7141 ggagaagtga agaagatagt tggtccgagc tttacatact gggcgtttgt acctaatccc
     7201 ttaccaccaa agctcgtata tgacaatgac cttctgtttc ttctcggtga agcaaatcga
     7261 gcgatgggag agttttcggg gtttctttct gggagtccag gaacgcgatt gttgatcagg
     7321 cctctctttc gcagggaagc cgttgcttcg tcacagatcg agggaacgac ttcggacatt
     7381 gaggatctct acttttttga agctgaccaa aatactgttc tcctggcaga agatgttcgg
     7441 gaggtgtcaa attatctaaa ggcacttgag tatggtcttg aacggctgga gtctttgccg
     7501 atttcaacac gacttttcaa ggagttacat tccgtgctca tgccgggagt gcgcggaggc
     7561 tattcatatc ccggagaatt aaggagaacg cagaactgga ttggaaaacc gggctgcact
     7621 ttgaatgaag ctgattatgt acctccgcct cccgaagagc tgaacgattg tgttggcgaa
     7681 ctggagaatt tcatcaactc cgaatctaga ctacctccac ttgttaggtt ggctttggtt
     7741 cattatcagt ttgaagccat ccatccattt gtggacggaa atgggagggt tgggaggcta
     7801 ttgatctctt tgctcaagac ttactgggga ctgatggatc aaccgggatt gtatctcagc
     7861 ggctatttcg aagctaatcg acaggagtac tattcaagat tgagaagcgt aagctttgag
     7921 ggcgactgga atggatggat tttcttcttt ctcaaggcgg tccttcagca gtctgagcta
     7981 ggaaagaata agctgaagga gatcatggat cttagaagca actggatagg tcgtctaaaa
     8041 cctgtttctt caccccttcc actagcgcta attgattctc ttttcgagaa tcctgtaatt
     8101 acaattccgc aagctgctga aagacttaac gtgacttacg ttagcgccag aagggcgatc
     8161 actgctttgt gtgagaaagg agtcctttca caaacagatc aaagcaagta cgggaagaaa
     8221 tacattgctt cagagttgat tgagattgtt aaatgaacac tcttcctcca ggaatatcaa
     8281 gagtattgga gatgctgcct ttggcaggaa gcattctcag gtccaccgtc aaaggttctc
     8341 cgtcctccga atcgttcgtt tccactatcg agatgaaatc ggtgtttatt tcggggaagg
     8401 tcgaaagttg ataacacacc ttgcaacgct tcagacgtaa ggaatctcac ttcttgttgg
     8461 gtggatacct ttatggattc tctcgtgaac tgaagatgga tgcagcagaa gagcttgaag
     8521 cgggacccta tctcttgaga atctttagtg cctggagtgt tagccagcga gaaggttctt
     8581 tcttttgacc gaactcccag cctttccagg ccatccatat cgattcaggt atgaatcttc
     8641 ccgaaggagt ctgtttggtg gctattatgt ctttcatttc actaaatcgt cggtcgtcac
     8701 ttttcagtga agatctcgag actacatcca gaacatgaag tatgtcgaac cagatttcag
     8761 gagatttgag cttacagaaa tctgtgccca tataaaatat atacggatga taatctcttc
     8821 ttctttccca tagtgagaga agtgactcta cgccgagttc gacttctgta cttcccagga
     8881 attcagggat ttctagtagg agcttcgtca tgacgagagt ggcgtacgga caagggtctt
     8941 ctcttcttcc aggtccgcgg aagcttccga gctcaggtga cgcgacgcat ggccagccat
     9001 tgtctcttat cagttttgct agatattcgg cggctccaag cactcttgca tcttcttgaa
     9061 gtcccattct tgctagcgaa tagagtacta ccggggcatc acacagcgcc caggcaaact
     9121 gatcagttcc caaaccgccg aattttacag ggatattcat gagaacctca aagggaccct
     9181 gtgaagagcg atgctcaagt atcttctcga tgatttcttc aaccccggga tcattttttc
     9241 ttatcccaag atcggccaga aatgtcaatt tgtgaatcag atggccggca ttcttgtggc
     9301 ttgtaaggac tgagccgggc cagtcttgaa gttcggcgat caatgatttt atctcagata
     9361 agttgagcat tttcatacgt gattcaacaa cgtcttcttc ctcttcatcc tgtgagagga
     9421 gatctaatct tgttctatat tcgatccatg aaggaccttc aaggagccaa tctataatct
     9481 cgtacatgat tcgcctccca aagagatgaa aagataactt gatggtttac tcagatctta
     9541 acaccgaaat ctatcaatgc aactgaataa ccgacctatt atgtgtagat tgaaaccaag
     9601 gcgagttttt ttctacaaaa atggtctttc cagcgtgaag ttgtgataaa atcacgatag
     9661 tagacggagt caagattgta ccagatacct gtgaacagga ggggtgattg ttatgtcaag
     9721 aactgtattg atgctcgccg gtgtggtagt cctggttatg gctcttctag cttcattcaa
     9781 cgtcggaggt attgtaatac ccgcctggta cgtttcattg aaatggatag gcggtgctat
     9841 tatgatcctt gttgcttata tggataaggg caagaaggct aagtcggact cttagtatta
     9901 caaggcccgg aggattctcc gggccgttta tttgtgatat ctcgttatga atcctttcgt
     9961 ttgaagtttc actttcttat ttcaagatct ttcagtatct ttggcgcttc atgaagctct
    10021 gaatttggca catcatactg gtcgccacgg ctgtctttct cttcaacgaa gaactccggt
    10081 attacattgt gcctgcgaat caaattatat tctgaggctt tgaggcgcta tgaccgttct
    10141 ttttctttaa gcggtttgac tagttttttg atttcggggg agtcgactcc tgacttttcg
    10201 attgccaaga ggatttctcc aatagcggga ttagatctct gaccgtcacc aagattaggt
    10261 actctgctca gtctgtctag ttctctttcg attgtttcaa cgactttcat aagaacagat
    10321 gtggaggttg ttcgaagaga gttagagatg atcttcgcag tttctgcttt cagaacggga
    10381 tttatcagcc acccattatc caggacgatc ctaacacggt catcgtcaga gcacgaaggg
    10441 tcttcggata cagcataaag cagtatacgc cacaggatag gggccctgga tcgtctccac
    10501 cattcgtaat agaagttcct gtctttgttg tcggttttta tcaagtgaag gatcgtatca
    10561 cgtacgaaat ctatatagat atgaagccat tccgggctcg attccggctc gctctcttcg
    10621 atggtctttc tcgcgctgca aatgctgtcg tactctctta ccgagccgtt gattaccgac
    10681 atgtctgtga cggccttcat tagctgcgag gtaagaatcg aaaggatgtc ttcatgtagt
    10741 atatcaaggt ttggttcaat gatctcggac caaaatttct tcaagcaatc ggttttgacg
    10801 agaggctcaa gatcgtattg cctgtgaaag agggaaagct tcgccggttt tccagtctga
    10861 gaaagagaga agaagctctg tatggccttc gtgattatcc atgggtttgt ggtgacatcg
    10921 accttcttca gggctttcag cattgcgaga catggagttt ctcctttgtg ccagttgttg
    10981 aggatcagat tcaaccattc tgcctgacag gtatctggaa tctccagatc actgtctgcc
    11041 atttgttcca cgatcgaaga gaccagatat gggttcaagg atgatcttct agaggccaag
    11101 aaagtcaggt gttcctggct gctgcttacg aaagcagttt ttacgaacca gcgggttaga
    11161 gacttttcga tctctgtgag cggctcttcc tcgcggaaga gattatccag catcgctttc
    11221 gaatcgagcc attccaccca tgcgaagccc tcagcgaaac gacaaaagac ctctgtgagg
    11281 tcttcacggt tgagacagta tgttaggtaa ctgtcgacct tttgatctat tccgggtttc
    11341 ttgccggcaa tgcttttaat tctgtcggta tggatttcaa gatctgaact ggccagggaa
    11401 gtccagtttt caagagcctt ctgaagttcg taatgaactt tcttcttttc gggtgttagg
    11461 ggataggaga cgggaatcac attgatctct ttccacctct cggcatcgct cgcgctgaca
    11521 agtgcgtatc tattcgattg ccttatgtcc agtcctcttg ccagataatc gactatggta
    11581 tctctgtgac tgtaccctat gaaaagaatc gtgtagtccg ataggtacag atccctaaga
    11641 aagtccctcg cccaaccttc ggttaggtaa gctcttccga agtccctgtt cgtaacgaca
    11701 agccatctag aatcctgttc cacgtttccg tgaagatata ctattcccct gaagtcatcg
    11761 cccctcggta gagcgggcgc tctgaagata tcgaagccgt cgtatctctc tccgtaattc
    11821 ttcaaaacgg ttagaaagtg cgcgtcgtaa tttgtcgtta cgattttgag gtcccgggcg
    11881 ctcttgaata gaagaggaat aaggttgtga agctgcgacg gtctagaggt ttctctactc
    11941 ataatctgtt ttacgatttc gtggacgccc tgtccgccat tcttggagtt tagactgccg
    12001 agaaagacgt ccggaggttg gactcgctgc tgtttgacgt agtatttttg gccgtagctt
    12061 ttcgcgacgg cttttgcgag gtcgacaaaa ccgggtatgt ttgaaggaga ggccattgaa
    12121 acgccggcac ccgcgaagac taccagcctt tcttcctcct gcgcccttat taattctgta
    12181 ggaatatcta catggtctgt taagagcatt attcctcccg ccgtctctct ttctaataac
    12241 cttcattcac aaattgagac ccttgtataa agagcctcct tgatgcaatg atactccatt
    12301 gccgcaccct gtgtcgggga agatgcttaa tcgaagaggc tttacctaga gagaaaaacc
    12361 gaatctggcg tctttcatgc ctgtcttgaa cggtgggaaa atgcgtaatt ctcacgccgt
    12421 tttcttcgta tgtaactccg cccaccttca tgtagggaaa tttctttgcg aacaaatggg
    12481 atctagattt ggtttgcctg atgactgtga gacaagctcc aggtgacatc ttgaacatc
//
LOCUS       DAOQYI010000041        12435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_5554_length_12435_cov_72.279402, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000041 DAOQYI010000000
VERSION     DAOQYI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5554_length_12435_cov_72.279402"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..154)
                     /locus_tag="PKL93_08895"
     tRNA            complement(80..154)
                     /locus_tag="PKL93_08895"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Glu,seq:ctc)
     gene            238..1029
                     /locus_tag="PKL93_08900"
     CDS             238..1029
                     /locus_tag="PKL93_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNQ71413.1"
                     /translation="MFVRRWFSSGKRASVVICHGIGEHSGRYDGFATYLNGKGFDVLA
                     ADFPGHGMHSGTRGFIKSFDDFTSLVKEVADRVKKIQPELPLFLFGHSMGGLIATRVI
                     EEHPDLFSAAALSAPHLFSAKDSVKNLLPLISIIRRVAPKTTFSSSSRFTPADLSNNE
                     RAVQRYIADPYVHDRVSPNLFFGLEDSIEQALKEADRIMTPTLIVYGSADRVVDPVGG
                     KELYEKINVEKKMLEIPGGKHELFADEERRSQFFGAISSFFLEHI"
     gene            complement(1003..1902)
                     /gene="ribF"
                     /locus_tag="PKL93_08905"
     CDS             complement(1003..1902)
                     /gene="ribF"
                     /locus_tag="PKL93_08905"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNQ71414.1"
                     /translation="MYVACIGNFDGVHLGHRAIMQKTVNIAANLDLQSTAISIVYPWG
                     YYFPNFPGIIYPVAQRLELILATGIERVLTVNMSEIRYLEPEDYISRLMSEGMRAIVV
                     GSDFTFGNGAKGNIELLERISKEGDLRVEIVPDALYDGRRISSSWIRESIAKGDIGLT
                     NSLLGKRYSIRGKVYKDKQLGAKIGFPTANIHRGDERLVIPRSGVYIIMSTIDSRDYF
                     GLLNVGFRPTVNTSEEVKYEVYFFDYSGNLYNRNLELEFLEFIRPELKFDSLGELIEQ
                     IKHDERVARRWLEIHLNMLKKER"
     gene            complement(1904..2824)
                     /gene="truB"
                     /locus_tag="PKL93_08910"
     CDS             complement(1904..2824)
                     /gene="truB"
                     /locus_tag="PKL93_08910"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNQ71415.1"
                     /translation="MSDGIILVDKPVGITSHDLVNLLRRRLGTKKIGHAGTLDPFASG
                     LVISGVNKGTRILEYFLEMDKTYRTELVLGRITDTFDITGRTVEERKVPGFCFNEVFN
                     ALKSFEGEYLQVPPAYSAKKYNGERLYKLAREGKIINLPPKPVKIYSIDDIGISYEKV
                     TFTAKVSKGTYIRSLVMDIGYKLGCGATVTELRRISQGNFSVDSAHQIDDVSDFSIIS
                     LEDSIDFLPGLLLNDSESSNVLLGKQIYASGVAGVMGKFAKNDLIRIIGSDERLLAVA
                     RSERTSSFIKTLLAKNSLERVAKLEKVLGA"
     gene            complement(2817..3188)
                     /gene="rbfA"
                     /locus_tag="PKL93_08915"
     CDS             complement(2817..3188)
                     /gene="rbfA"
                     /locus_tag="PKL93_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNQ71416.1"
                     /translation="MASSYRKEMLESEIKKVLTVTLSSYTSYNDSLGMVSIVRVELTK
                     DKRFATVFVSLMGPDESKKKLVKRLNEDKGIFRTAIAKNIRLFKAPEIRFKEDIGIEA
                     SLRVAQLLEKIERERTNTKDE"
     gene            3575..4267
                     /locus_tag="PKL93_08920"
     CDS             3575..4267
                     /locus_tag="PKL93_08920"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNQ71417.1"
                     /translation="MCSERRKFVVILALFFSTFAFSLTICIDPGHQKEADLTLEPIAP
                     GSEMTKARVSTGTRGSSTGIPEYVFNLEFSFMLRDRLLQEGYEVVMTRDSHDVNISNV
                     ERAKIANEANADLCIRVHADYSSDSSLKGFMLLIPSETSAQSPLIYSDSKKAAEKIMA
                     SLLQIDGIDSLGIRPRDDITGFNWSEVPVLIFEAGFMSNPEEDVLLSTRDYREKLVEA
                     LVTGIEDYFLTK"
     gene            complement(4467..5774)
                     /locus_tag="PKL93_08925"
     CDS             complement(4467..5774)
                     /locus_tag="PKL93_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ71418.1"
                     /translation="MKKGKAAFVLFLLLFMMVFLNADQMVMSPNIGEIEAEFGITKAD
                     IGLIQGSFTIVGALISLLWGFLADKYSRKLLLLFSVLVGEIPCFLSAFVQTFPQLFLA
                     RALTGIGVGALFPVVFSYAGDAFKESQRAKVNSFLSTAISLGAIVGMVIAGFTGTSLG
                     WRTPFIIVSLPNIVLAFLFFAFAEEPKRGAAEVAVGELVDQGINYIGKVRLSDYKNLF
                     KVKTNLILFIQGILGTIPWGAIPYYLVNFFETSKNLSKESATLIFIFFGVGNVLGIFF
                     GGLIGGLLYKKKPAYMPLFSGIMTVIGTFVALLALNFPPIEGAGGFVMLGALGMVAAA
                     SASMTGPNMKTMLMNVNPPENRGRIFSIFNLTDSLGTGFGQFFAGTLATAVGSLGVAM
                     NVSALFWLPCGLILLTAALAFPRDIDHLHRKMKEAAQTMEKEK"
     gene            complement(5790..6686)
                     /gene="era"
                     /locus_tag="PKL93_08930"
     CDS             complement(5790..6686)
                     /gene="era"
                     /locus_tag="PKL93_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNQ71419.1"
                     /translation="MKSGTVALVGKPNVGKSTLINTIIGEKIAIVSDKPQTTRNRIGG
                     ILTSDDGQIVFYDTPGIHKPLHRLGQYILKVATSSLVGSDLLLVIVDPTDGLRESDRL
                     VSNHVNQSHIPVFLAINKIDEYEDKRLIEEFRQKAEQLFENISRTFFISAIKAMGVDE
                     LISAIFDFLPEGELLFPEDVITDRSSRFMASEVVREKILENTRQEIPHSIGVVVQEFK
                     DDGTILKIRADIIVERSSQKPIILGKAGSMIKKIGTDARKDLEYIFDQKIFLDLFVKV
                     REKWRDKDSMIQEFTNLKDELH"
     gene            complement(6694..8730)
                     /gene="glyS"
                     /locus_tag="PKL93_08935"
     CDS             complement(6694..8730)
                     /gene="glyS"
                     /locus_tag="PKL93_08935"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNQ71420.1"
                     /translation="MSDHKALLEVGIEELPSSEVTGIRDQLNDKIGEALENYHLRFDR
                     LEVFVASRRFGVLIHGIDGKQADFMEQRKGPSEKIAYKDGVPTKALLGFLRGSNAKLE
                     DVIVKDSYVYIEREITGETAEALLPQIFSDSLKSMDFKKPMRWGDGIYRFVRPVRWIV
                     AMLDSKTLEMELFGKKSSNKSRGHRFFFDEVEVSPYNYFEKLSEALVIARESDREERV
                     LSEIRRIESNLQSKIPVDKELVAEVVSLTEYPSAVLGRFLEKYLELPPEVIIVTIKHH
                     QRTFPVDKDGKLTNNFVAFQDGPDDPMGNIRQGYEEVINARLEDAFFYFIKDKEKPFE
                     AYVNALEGILFQRGLGSLKDKTDRTVKLSTEISRMLGANNDELLEVQRTALLAKADQT
                     TRVVQEFPELQGIMGRIYALYSKESHDVAMGIEEHYLDGSIPTTLTGAVVGVADRLDT
                     LVGNFMIGNIPSASKDPYALRRKASFIFRTMHHLGWKISLDSLISKAMENLGTISKES
                     DELIRDFFSSRFEAFLIEKGFSINISRSVNKWWNVPYLGVRAAEAIEEYIKKEDFNNL
                     LIAYQRVHNISKNHLENIFDGSKFVEQAERDLLNDYLRCYDDVMDALERDNFEKSLEL
                     LTSLKPQIDRYFDDVFVMADQEDIRLNRLGFLKSLDLLFLKIGDLSLLLEEERT"
     gene            complement(8723..9577)
                     /locus_tag="PKL93_08940"
     CDS             complement(8723..9577)
                     /locus_tag="PKL93_08940"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNQ71421.1"
                     /translation="MYLQDVIGKLNSYWSMQGCIIDQPYDLEMGAGTFHPSTFLRSLG
                     KKPWKVAFIQPSRRPTDGRYGENPMRVQRYFQYQVIIKPNPEDSQELYLGSLEALGIN
                     PEEHDIRFVEDNWESPTLGAWGVGWEVWLDGMEVSQFTYFQQVGGIDVDLVSLEITYG
                     LERITMYLQKKANIFDIDWNGEFKYGDVFLENEKEFSAYNFDVAETSRLFELYRLYRE
                     EFELCLNSGLVRPSYDYMIKCSHVFNLLDARNAISVSQRQSYIKSIREMAKAVAETYV
                     AKESDSDE"
     gene            complement(9600..11264)
                     /locus_tag="PKL93_08945"
     CDS             complement(9600..11264)
                     /locus_tag="PKL93_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNQ71422.1"
                     /translation="MKRINGKFFVAAFRKAAERLLANKDEINALNVFPVPDGDTGSNM
                     AAAMIEACEYLDRLKKDDLVSVLEAVKTGMLMGARGNSGVILSQIFRGFAEGIGNRKF
                     VNTKAFTEGLVKAKEIAYRSVMKPVEGTMLTVMRVTADTANEQFGDIEDFEEYFENLI
                     HVAFDAVEKTPTLLPKLKEAGVVDSGAKGLAYILEGFVLAVKGDLELEGPIQQMPQAM
                     GSSTERIVEIVREELKFTYCTELIVSLNDSENPEEVSEEMKSYLDAMGDSIVMVHQDD
                     IIKIHVHTDHPGDVIEKFLGVGLLQKVKIDNMKVQHEHIVDIQSKGPEMYGKGKRHGV
                     IVVSPGEGLADVLKSLGVDHTVKGGQTMNPSLKDLYEAINRIAADKVIVLPNNPNIIL
                     TAKEAANAIKDENPGKEVYIIPTRTVQEGIAAMTVYNDEMDSEALIAEMKEAADSISP
                     ISITYAVRDSSMKGKKIRKGEYIAIGRDGLISSGRKLEKLVHDSIKATIGKDSEKEVV
                     TIFYGSEVTEESAGKLLESLSGSFSDLEFEIHSGGQPYYYYLISIE"
     gene            complement(11280..11627)
                     /locus_tag="PKL93_08950"
     CDS             complement(11280..11627)
                     /locus_tag="PKL93_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNQ71423.1"
                     /translation="MQVTTEFGKIDISLQAISSIVKKVVSESYGPVNVGSPQTGFLAK
                     LFGTTEDSKKGIKVTEEIDGTLEIDIDLILEYGVRIPTVVENIQENVFHKLKELTEAT
                     NIKVNIHVVGLQD"
     gene            complement(11602..>12435)
                     /locus_tag="PKL93_08955"
     CDS             complement(11602..>12435)
                     /locus_tag="PKL93_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ71424.1"
                     /translation="HPARFFDLGITEQLCTTFAGGMAAAGVKPVFAVYSTFLQRALDQ
                     LIHDVALQDLPVLFAIDRAGIVGQDGATHNGIFDISFLSMIPNMRVLAPASLKELANM
                     LFTIFDQNWLTHPTAIRYPRETELADIDEITSSLKKIEIFKWEKLIDGNEIAILATGS
                     MVSRSREAAYINGCTLFNCRSIKPLDIETLRQIFSDYETIITVEEGIKSGGFGSNVVL
                     EAIRHNYSGRIDLIAIDDAFSSHGTREEVLNELGLDARGIENRISKLRGEKNASNYRI
                     R"
BASE COUNT         3250 a         2940 c         2573 g         3672 t
ORIGIN      
        1 caaaaaagag ctgaaaaagc gaaaatctcc caactgggag attttcaatg gaacttacgc
       61 cgatggagaa ctaaaatgtt ggcagcccca cggggaatcg aaccccgacc ttcggactga
      121 gaatccggtg gactagccgt tatcctatgg ggccgaacag aaataatatt atcatgctct
      181 ctggcttttg taaacacccc tgttgaagag atataatcag tttgaggagg tgttgcggtg
      241 tttgttagaa gatggttttc ttcgggcaaa agggcaagcg tagtgatttg tcacggaatt
      301 ggtgaacaca gtggaagata cgacggtttt gctacttatc ttaacggaaa gggattcgat
      361 gtgcttgcgg cggattttcc aggtcacggc atgcattcgg gaactcgggg tttcattaag
      421 tcatttgacg acttcacgtc gcttgtaaag gaagttgccg atcgagtgaa gaagattcag
      481 cccgagcttc ccctcttcct tttcgggcac agtatgggag gacttatagc tacaagagtc
      541 atagaggagc atccagatct tttcagtgcc gccgccctca gcgctcccca tctcttttcc
      601 gcaaaggatt ccgtcaagaa tctgcttccg ttgatttcga ttattagacg agttgcacca
      661 aaaactacat tcagtagttc ttctcgtttc actcccgctg acttgtcaaa taatgagaga
      721 gctgtacaac gatacattgc agatccttat gtgcatgatc gagtatcacc gaatctcttt
      781 ttcggtcttg aagatagtat cgagcaggcg ctgaaagagg cggatagaat aatgacacct
      841 actctaatag tctatggttc cgccgacagg gttgttgatc cagtaggagg aaaggagctc
      901 tacgaaaaga ttaacgtgga gaagaaaatg ctggaaatcc cggggggaaa gcacgaactg
      961 tttgccgacg aagaaagaag gtctcaattc ttcggggcga tttcatcgtt ctttcttgag
     1021 catatttagg tgtatctcca gccagcgacg ggcaactctt tcatcatgtt ttatctgttc
     1081 tattagctcg cccagtgagt cgaatttcaa ctcgggccta ataaattcaa gaaactccag
     1141 ttcaaggttt ctattgtaga gattgccgga atagtcaaaa aagtatactt catacttgac
     1201 ttcttccgat gtgtttaccg tgggtctgaa accaacattt agcaaaccaa agtaatctct
     1261 agagtcaatc gtcgacataa ttatgtagac acccgatctt ggtattacta gcctttcatc
     1321 tccccgatgt atgtttgcag tcggaaaacc aatcttggcg ccaagctgct tgtctttgta
     1381 aaccttgcct cgtatcgagt atctctttcc tagaagggaa ttcgtgaggc cgatgtcccc
     1441 tttagctata gattccctta tccagctgct gctaattctg cgcccatcat atagcgcgtc
     1501 gggtacgatc tcaactctca agtctccttc ttttgagatc ctctccaata gctcgatatt
     1561 tccctttgca ccgtttccaa aggtgaaatc acttccgact acaattgctc tcatgccttc
     1621 actcatcagc ctggagatgt aatcttccgg ttcaagatac ctgatttctg acatgttgac
     1681 agttagtacc ctttcaattc cggtagccag aatcagttca agtctctgtg caacaggata
     1741 gattatcccc gggaagtttg gaaagtagta tccccaggga tagacaattg agattgctgt
     1801 actctgcaaa tctagattgg ctgctatgtt taccgtcttt tgcatgatcg ctctgtgacc
     1861 caaatggacg ccgtcgaaat ttcctatgca ggcaacatac atatcaagca ccaagaacct
     1921 tttccagttt tgccactctc tcgagcgaat tcttagctag gagtgtcttg atgaatgaag
     1981 aagtgcgctc tgatcttgcg actgccaata gtcgttcgtc gctaccgatt attctgatta
     2041 agtcgttctt cgcaaacttt cccataacac ctgccactcc gctggcatag atctgtttgc
     2101 caaggaggac attgctgctc tcagaatcgt ttagcagaag acctggaagg aagtcaatcg
     2161 aatcttcgag tgaaattatt gagaaatcag acacatcgtc gatctggtgt gcagaatcaa
     2221 cggagaaatt accctgagaa atccttcgta gttcagtaac cgttgcgccg cagccaagtt
     2281 tgtacccaat atccataacc aacgatctga tgtaagttcc ctttgagacc ttagctgtaa
     2341 aggtgacttt ctcataagaa atgccaatgt catcgataga atagatctta accggttttg
     2401 gcggaaggtt gattattttg ccctctctag cgagtttgta aagcctctct ccgttgtact
     2461 tctttgcaga gtatgcggga ggaacttgaa gatactctcc ttcaaatgat ttgagtgcat
     2521 taaaaacctc attgaaacag aaacccggca ccttccgctc ttcaacagtc ctgcccgtaa
     2581 tgtcaaatgt atctgtaatt cttcctagca cgagctcggt cctatatgtt ttgtccattt
     2641 caagaaagta ctcgagaatc cgagttccct tgtttacccc agagataacg agcccactgg
     2701 caaaagggtc cagtgttccc gcatggccta tcttctttgt ccccagtcga cgtcgaagga
     2761 ggttgacaag atcgtgtgag gtaattccga ccggtttgtc tacaagtata atcccatcac
     2821 tcatctttcg tgtttgtcct ctccctttcg atcttctcca gcaactgagc tactctcaag
     2881 cttgcttcta ttccaatgtc ttctttaaaa cggatttcag gggctttgaa cagacgaatg
     2941 ttctttgcaa ttgcagtcct gaagattccc ttatcttcat tcagcctttt aaccagcttt
     3001 ttcttgcttt catctggtcc cataagactt acaaagaccg ttgcaaaacg cttatctttg
     3061 gtaagttcta ccctgactat tgaaaccatt cctagcgagt cgttgtaact tgtgtaagat
     3121 gatagagtta ccgtgagaac cttctttatc tcagactcca acatctcttt cctgtaacta
     3181 cttgccatgg ctctcacctc cgggataatt ataacctaca acggagctaa gagggagttc
     3241 cttgggaagt agcggtttta gcgaaatgta catccattat tagctgcaat tttacttaga
     3301 aacggttttc agtaacttgt tgactgaagg acaagaatcc accaagaaat tggatgatat
     3361 tgaatgaacg atgtattaaa cgcaattgat atttattggg cgggcatgac acagtgcgcg
     3421 ttctgttgtt agatcaaaaa tatgattccg ataattggag agagtgattg tgcctccctg
     3481 agcttgcata aacggaactc tttgccgata gcttaacccg attaatctgt aaagaagtta
     3541 taatctgtag tgatcaacag ttaggagtga ttctatgtgt tcagaaagaa gaaagtttgt
     3601 cgttattctt gctctctttt tttccacatt tgctttttcg cttactatct gcatcgaccc
     3661 cggtcatcag aaagaagcgg acctgactct tgaacctata gctccaggtt cggaaatgac
     3721 taaggcaagg gtttccactg ggacacgcgg atcctcgacc ggtattccag aatacgtttt
     3781 taatttggag ttctctttca tgttgaggga taggcttctt caggaagggt atgaagttgt
     3841 catgaccaga gatagtcacg atgtcaacat aagcaatgtt gaaagggcga agatagcaaa
     3901 cgaggcgaat gccgatcttt gtattcgagt ccatgctgac tatagctccg atagcagcct
     3961 caaaggattc atgttactaa ttccatctga aacctctgct caaagtccat tgatctattc
     4021 ggacagcaaa aaggccgcgg aaaagatcat ggcaagtcta ttgcagatcg atggtataga
     4081 ttcacttgga attaggccac gcgacgatat tactggattc aactggtcag aagttcccgt
     4141 actaatcttc gaagcgggct ttatgtcaaa tcccgaagaa gatgtccttc tctctaccag
     4201 agattacagg gaaaagctcg tggaagctct cgtcacagga attgaggatt actttctgac
     4261 gaagtaatgt gattcaaagg tcgaacagaa aggatctttt gtcatggttc ttttctggtt
     4321 gttcctcaat aaataaaaat tgctgccctt ttcgagggca gcgaagcggt ctgagggtta
     4381 cttcagcaat tagattatac aactttcttc tcgatattcc aaatcaaagc cactcctgaa
     4441 atcatctttc acctcttgac agacatctac ttttcttttt ccattgtttg agcagcttcc
     4501 ttcatctttc tatgaaggtg atcgatgtct cttgggaaag ctaatgctgc agtaagcagt
     4561 atcagcccgc aaggcagcca gaatagagcg gaaacattca tagcaactcc aagagatcct
     4621 actgccgtgg ctagagttcc agcaaagaac tgtccaaatc ccgttccaag agaatctgtc
     4681 aagttaaaaa tcgagaatat ccttcctctg ttctcaggag gattgacatt catcagcata
     4741 gtcttcatgt ttggtcctgt cattgaagcg gaggctgcgg ctaccatgcc cagggctccc
     4801 agcatcacaa atccgcctgc tccctcgata ggtgggaagt tcagagccaa tagagcaaca
     4861 aatgtcccta tgacagtcat tatcccgctg aagagcggca tgtaggcagg tttcttcttg
     4921 taaagcaaac cgccgatgag accaccgaag aatatcccga gtacgtttcc gactccgaag
     4981 aaaatgaata ttaacgtggc cgattccttt gaaagattct tagaggtttc gaagaagttt
     5041 acaaggtagt acggtattgc tccccaggga atcgttccaa gaattccctg aatgaagaga
     5101 atgagattgg tcttcacctt gaagagattc ttgtagtcag acagtctgac ttttccaatg
     5161 tagtttatcc cttgatccac gagttcgccg actgcaactt cggcggctcc tctttttggt
     5221 tcttcggcaa atgcaaagaa tagaaatgct agaacaatgt ttggaagaga tacgatgatg
     5281 aacggtgttc tccagccaag tgatgttcct gtgaatccgg cgattaccat tcccacaatg
     5341 gctccaaggg aaattgccgt agaaagaaag gaattgacct ttgctctctg agactctttg
     5401 aaagcatctc cggcgtaaga aaaaacgact gggaagagcg ctcctacgcc tataccagtt
     5461 agggcccttg caagaaagag ctgcggaaaa gtctgtacga aggcggagag gaaacagggt
     5521 atttcaccga ctagaacact gaaaagaagc agaagctttc tgctgtactt gtctgccaga
     5581 aaaccccaga gaagagaaat cagtgcgccc actatagtga aagaaccttg tataaggccg
     5641 atatctgcct tagttattcc gaattccgcc tcgatttcgc cgatgttggg agacatcacc
     5701 atctgatcag cattaagaaa gaccatcatg aagagcaaaa gaaagaggac aaaagctgcc
     5761 tttccctttt tcatctgcat caccccgatt taatgaagtt cgtctttgag gttagtaaac
     5821 tcctgtatca tggaatcttt gtctctccat ttttctctaa ctttgacaaa caaatccagg
     5881 aaaatctttt gatcgaaaat atactcaaga tctttcctcg catcggtacc gatttttttt
     5941 atcatcgatc ctgccttacc aaggattatc ggcttctgac tgcttctctc aacaattata
     6001 tctgccctga ttttcagtat agtgccgtcg tctttgaact cctgaaccac aacgccaata
     6061 gagtgcggga tctcctgccg ggtattctca aggatctttt cgcgaacgac ctccgatgcc
     6121 ataaatctcg aagaccggtc agttatgaca tcctcgggaa aaagtaactc tccctccgga
     6181 aggaaatcga atattgctga aatcagctca tcgactccca tagcctttat cgctgaaatg
     6241 aagaacgtac ggctgatatt ttcgaagagc tgttcggcct tttgtctgaa ctcctctatg
     6301 agtctcttgt cttcgtactc atcgatcttg tttatggcga ggaagacagg aatgtgactt
     6361 tggttcacat gattcgaaac cagcctgtcc gattctcgaa ggccgtcggt tgggtcaaca
     6421 atgaccagca gtagatcaga ccctactagc gatgaagttg caactttcag tatgtattgc
     6481 ccgagtctgt gaagcggttt gtgaattcca ggtgtgtcat agaagactat ctggccgtca
     6541 tcagatgtca ggatcccccc gattcgattt ctagttgtct gaggtttatc agaaacaata
     6601 gcgatcttct cgccgatgat ggtgtttatg agggttgact tccccacatt gggttttcca
     6661 acaagagcaa cggtcccgga cttcattgac ggttcacgtt ctttcttctt caagaagaag
     6721 cgaaaggtcg cctatcttca ggaagagcag atccaacgat ttaagaaagc cgagtctgtt
     6781 aaggcgaata tcctcctggt cagccattac aaagacatcg tcaaaatatc tgtctatttg
     6841 tggcttgaga gatgtgagaa gctcaagaga cttctcgaaa ttatctcgct ctaaggcatc
     6901 cattacatcg tcataacacc tcagatagtc gttcaacaga tcacgttcgg cctgttccac
     6961 aaactttgag ccgtcgaata tattctccag gtgatttttg cttatgttgt gaaccctctg
     7021 ataagcaata agaagattat tgaaatcttc cttttttatg tactcttcga ttgcttctgc
     7081 cgctcttacc cctagatatg gaacgttcca ccacttgttc acggatcgag atatattgat
     7141 tgaaaagcct ttctcaataa gaaatgcttc gaatctagat gaaaagaagt cccgaatcaa
     7201 ttcatcggat tctttagaaa tcgttccgag attttccatg gctttgctta ttagactatc
     7261 cagggagatc ttccagccga gatgatgcat agtccggaag atgaagctag cttttctcct
     7321 cagcgcataa ggatccttcg aagcggaagg tatatttcct atcatgaagt tgcctacaag
     7381 cgtatccagc ctatctgcaa cccctacgac tgcgccagtc agtgttgtgg gaatggagcc
     7441 gtctagatag tgctcttcaa ttcccatagc aacatcgtga ctctctttgg aatacaacgc
     7501 atatatcctt cccattatgc cttggagctc ggggaactcc tgaacgactc tggtggtttg
     7561 atccgcttta gcaaggagag ccgttctctg aacctccaga agttcgtcgt tatttgctcc
     7621 gagcatcctg gaaatctctg tggaaagctt aactgttcta tcggttttgt ccttcagcga
     7681 gccaagacca cgttgaaaca aaattccctc cagcgcattg acgtacgctt cgaaaggttt
     7741 ctctttatcc ttgatgaagt agaaaaaagc atcttccagc cttgcattga tgacctcttc
     7801 ataaccctgt ctaatattac ccatcggatc atctggacca tcctgaaaag ccacgaagtt
     7861 attcgtcaac ttgccatcct tgtcaaccgg gaaagttctc tgatgatgct ttatggttac
     7921 gattattact tcaggcggga gctcgaggta cttctccaag aaacgcccaa gaacagcgga
     7981 gggatattcc gtcaaggaga ctacttcggc aaccagttct ttatcgacag gaattttgct
     8041 ttgaagattc gattcaattc ttctgatttc tgagagaact ctctcttccc tatcgctttc
     8101 tcgagcgatt accagagctt cactcaattt ctcgaagtaa ttgtaaggtg atacttcaac
     8161 ttcgtcaaag aagaacctgt ggccccttga cttgttcgat gacttctttc caaacagttc
     8221 catctcaagc gttttggaat caagcatcgc tactatccat cttacaggtc tcacgaatct
     8281 gtaaatccca tctccccagc gcatcggttt cttgaaatcc atggacttca acgagtcgga
     8341 gaagatctga ggcaaaagag cttcagcagt ctcgccagtt atttctctct caatgtaaac
     8401 atagctgtcc tttactatta cgtcctctag cttcgcattg cttcctctca aaaaacctag
     8461 aagcgccttt gtcggcacac cgtccttata tgcgatcttc tcggacggtc ctttcctctg
     8521 ctccatgaaa tcggcttgtt ttccatcgat accgtgaatc agcactccga atcttctgct
     8581 cgcaacgaat acttccaatc tgtcaaaccg cagatgatag ttttcaagag cctctccgat
     8641 cttatcatta agttggtctc tgatgccagt cacttcactt gatggcagtt cttcaattcc
     8701 gacttctagc agagctttgt gatcactcat ctgaatcgct ctcctttgct acgtatgttt
     8761 ctgcaactgc ctttgccatc tctctaatgg attttatgta gctctgacgc tgtgaaacgc
     8821 tgattgcatt tctcgcgtca agcaaattga agacgtgaga acatttaatc atgtagtcgt
     8881 aagaaggtct gacaagacca ctattaaggc agagctcgaa ttcttcgcga tataatctgt
     8941 acagctcaaa aagccttgaa gtctcggcca catcgaaatt gtaagcggag aattctttct
     9001 cgttttcgag aaagacgtct ccatacttga attctccgtt ccagtctata tcaaatatgt
     9061 tcgctttttt ctgaagatac atggtgattc tttccagtcc ataggtgatt tccagagaaa
     9121 ctaaatcaac gtctatgcca cctacctgct ggaaataagt aaactgactg acttccattc
     9181 cgtctagcca gacttcccag cctactcccc aggcgccgag agtcggagac tcccagttgt
     9241 cttcaacaaa tctaatgtca tgttcctcag gattaatgcc cagtgcttct aaagaaccga
     9301 gataaagttc ttgcgagtct tcgggatttg gctttattat tacctggtac tggaagtatc
     9361 tttgaactct cattggattc tcgccatatc ttccatctgt tggtcttcta ctgggttgaa
     9421 tgaaagctac cttccagggt ttcttcccaa gtgacctaag aaaggtcgaa ggatggaagg
     9481 ttccagcccc catctctaga tcataaggct gatctatgat acatccctgc atagaccagt
     9541 atgagttaag cttccctatc acgtcctgta agtacattct tccctcctga gctactagtc
     9601 tattcaattg agattaagta gtaataatag ggctgtccac cactatgaat ttcgaattca
     9661 agatctgaga aacttcctga aagggattct agaagctttc cagcactttc ctcagttact
     9721 tccgatccat aaaatatcgt tacgacttcc ttctcactgt ccttaccaat agttgccttg
     9781 atcgaatcgt gaacgagctt ctcaagtttc ctgccagagc ttatcaggcc gtctcttcct
     9841 attgcaatgt attcgccttt tctgatcttc tttcctttca tgctagaatc tctcacagca
     9901 taggtaatcg aaatcggcga aatcgagtcg gccgcttctt tcatttctgc aatgagtgct
     9961 tcactatcca tttcatcgtt atatacagtc atagccgcaa tgccttcttg cacagtacga
    10021 gttgggatta tgtaaacctc tttcccggga ttttcgtcct taatagcatt agcagcctct
    10081 ttggcggtta gaatgatatt cgggttattg gggagcacaa tcaccttatc tgcagctatt
    10141 ctatttatgg cttcgtaaag gtcttttagg ctcggattca ttgtctgacc gcccttaact
    10201 gtgtgatcta caccaaggct cttcagaacg tcggcaagac cttcaccagg ggagacgaca
    10261 attactccat gacgcttacc tttaccatac atttctggtc ctttcgactg aatatcaact
    10321 atgtgctcat gttgaacctt catgttgtca atcttaacct tttgaagtaa tcccacccct
    10381 aagaacttct cgatcacatc tccagggtgg tcggtgtgga cgtggatctt aataatatca
    10441 tcttgatgaa ccataactat cgaatctccc attgcatcaa gataactctt catctcttct
    10501 gatacttctt cagggttttc tgagtcgttg aggctcacaa ttagttccgt gcaatatgta
    10561 aatttaagtt cttccctgac gatctcaact atccgttccg ttgacgaccc catcgcttga
    10621 ggcatctgct gaataggacc ttcaagctct agatcgccct tcactgcaag cacaaaacct
    10681 tcgagaatat aagccagccc ctttgctcca gagtcaacaa cgccagcttc tttaagtttg
    10741 ggaagtaaag ttggagtctt ttccactgca tcgaaagcga catgaattag attttcgaag
    10801 tattcttcaa aatcctcaat atctccgaat tgttcgtttg cggtgtcggc cgttactctc
    10861 attacggtga gcatggtgcc ctcaacgggt ttcattacgg atctataggc aatttccttt
    10921 gctttaacta gtccttcagt gaaggctttg gtgtttacga attttctgtt tccaattccc
    10981 tcggcaaatc ctctaaaaat ctgtgagagg ataacgccag aattgccacg ggcgcccata
    11041 agcattccgg tcttgactgc ttccagaacg ctcaccagat catctttctt caatctatcc
    11101 agatattcac aggcctctat cattgcagcg gccatattag atcccgtgtc tccgtctgga
    11161 acgggaaaga cattcagcgc gttgatctca tctttatttg caagaagtcg ttcagcggct
    11221 ttccggaagg cggcgacaaa aaacttgccg ttgatacgtt tcatatcttg agcctcccat
    11281 caatcctgca agccgacaac atgaatgttc acttttatat tggtagcttc agtaagttcc
    11341 ttaagcttgt gaaatacgtt ctcctggata ttttctacaa ccgttggtat tcttacgcca
    11401 tactctagaa ttagatcgat atcgatttca agggtgccat cgatttcttc tgtcactttg
    11461 ataccttttt tcgagtcttc tgtcgttccg aataactttg ccaaaaaacc cgtttggggg
    11521 gatcctacat tgaccggtcc gtaagactcc gatacaacct tcttgactat tgaagaaatg
    11581 gcctgaagag aaatatcgat cttaccgaat tctgtagtta cttgcattct tttcacctct
    11641 cagtttagag attctattct ctattcccct ggcatccaac cccagctcgt ttaacacctc
    11701 ttctcttgta ccgtgagaag aaaaggcatc atcgattgca attaaatcga ttctaccact
    11761 gtagttatgt ctgatagctt caagtaccac atttgagccg aagcctccgc tctttattcc
    11821 ttcttctact gtgattattg tctcgtaatc cgaaaagatt tgccttaaag tctcaatgtc
    11881 cagtggtttt attgaacgac agttgaaaag ggtacatccg ttgatgtaag cagcctctct
    11941 gcttctggaa accatagaac ctgtagcgag aattgcgatt tcatttccgt caataagctt
    12001 ttcccactta aagatttcta tcttcttcaa gctggatgtt atctcatcaa tgtctgcgag
    12061 ttctgtttcc ctggggtacc tgatggcagt cgggtgagtc aaccaattct ggtcaaatat
    12121 ggtgaatagc atgttggcga gctctttcag tgaagcggga gcaagaacac gcatattcgg
    12181 aatcatcgaa agaaaagaaa tgtcgaagat cccgttgtga gtcgcaccgt cctgaccgac
    12241 tatgcccgcc cgatcaatag caaataagac ggggagatct tgaagagcta cgtcatgaat
    12301 tagctggtca agagctctct gtagaaaagt cgagtagaca gcgaagacgg gttttacacc
    12361 tgccgcagcc attccacctg caaaagtagt acacagctgt tcggttattc caagatcgaa
    12421 aaaccttgcc gggtg
//
LOCUS       DAOQYI010000042        12326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_5623_length_12326_cov_78.250917, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000042 DAOQYI010000000
VERSION     DAOQYI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 12326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12326
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5623_length_12326_cov_78.250917"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /gene="heR"
                     /locus_tag="PKL93_08960"
     CDS             <1..608
                     /gene="heR"
                     /locus_tag="PKL93_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HNQ71425.1"
                     /translation="ATENFMDLKMGPFIASFLFMSAIAHFIVSTFGFGWYARNLQRGI
                     NYARWYEYSISSSVMIVAIAMFSGMLDIVSLMLLFTVNALMNLFGLMMELHNQTTERT
                     NWTAFIFGCIAGAVTWIAVFIYLFTSLGKANIEFPSFVYAILITFLVFFNSFAINMFL
                     QYKKIGPWKDYLFGESMYVLLSLVAKSVLAWQVFAGTLRPV"
     gene            680..1417
                     /locus_tag="PKL93_08965"
     CDS             680..1417
                     /locus_tag="PKL93_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ71426.1"
                     /translation="MPNLYFYAGSTNPELYELQNKLIDPERKIEKVMEEFCDFESGVI
                     VDVGAGSGFHSHMYASTASMVYAVEPDSGMLKQLISRQARDFRSNFCVVKGFAEDLPF
                     RSDLADIVHSRLAYFFGPPMKYTESCEEGIVEVKRVLKTDGYFFNIQNNYSSGQYAEF
                     LELSYGRDLKNLQKEISDYFVSRGFLERVVRTEWRANNRGEIEEALTLEFPIEKVGQI
                     MKAFSGNEFSHDFSIFVFRKRISGENR"
     gene            1644..2135
                     /locus_tag="PKL93_08970"
     CDS             1644..2135
                     /locus_tag="PKL93_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNQ71427.1"
                     /translation="MALQFISLLVISFIIWLGFTGSFALAEILLGLVVSFVVAIVLSK
                     YSRFRIGLDFPVRLFRFVFSFLPIFIFEMVKANIDVAKRVLNPSLPINPSMVEVETDL
                     KGEISKLTLANSITLTPGTLTVDIGEDRLLVHWIDKKADEPERIREEISGKFEKRIGG
                     IFE"
     gene            2132..2386
                     /locus_tag="PKL93_08975"
     CDS             2132..2386
                     /locus_tag="PKL93_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ71428.1"
                     /translation="MIGFSIFFSLILIGAGLTLYRLLRGPTVPDRVAALDILNVMITS
                     AIVLFSLIDGNPLFLDIALVYAALSFLETIVVARYLEGRR"
     gene            2383..2727
                     /gene="mnhG"
                     /locus_tag="PKL93_08980"
     CDS             2383..2727
                     /gene="mnhG"
                     /locus_tag="PKL93_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNQ71429.1"
                     /translation="MIILDILGYALLSIGAFFFFMGGLGMLRMPDVFNRLQAGTKATT
                     LGSFSVILGVGLINPQWLLKTIIIVAFIAITNPVGSSAIARTALKKGITPIAYEESHK
                     KADSLPEGGEEE"
     gene            2724..2966
                     /locus_tag="PKL93_08985"
     CDS             2724..2966
                     /locus_tag="PKL93_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNQ71430.1"
                     /translation="MNVFSFIIGVVMVGLAIFAIEAKKLLTSVIMLSAMSLLSVILFV
                     IMKAPDVAITEASVGAGLTTAIFLLSLRKLKRADVE"
     gene            2963..3721
                     /locus_tag="PKL93_08990"
     CDS             2963..3721
                     /locus_tag="PKL93_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HNQ71431.1"
                     /translation="MKRMISALIALLFFFMIVTVLNSDSSDSMGIPRYGEVKLSERVS
                     AKYIEKSVNGNEDEINFGVTADAETGSANMVTSIVVNYRSFDSLGEVTVLFISATGVG
                     LLLGGGRKRAGSAISVSPVVKIAARVIAPLLMVLGIYVFVHGHLTPGGGFPGGAIIAA
                     GFLLLVAVDDEKRASKGLKILEGTMGLLYVLIGIAGLLISGSFLKNFLPTGVVGELFS
                     AGIIPVVYSVIGIKVGAELSGIVDDFISEGGDEA"
     gene            3718..>3944
                     /locus_tag="PKL93_08995"
     CDS             3718..>3944
                     /locus_tag="PKL93_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HNQ71432.1"
                     /translation="MIQYFSMMLVAVGLYGLLSQKNLVKLIISLNIAEIGVNLFIVSI
                     GYIEGGAAPILSSVSDNSSLLFVDPLPQALVL"
     assembly_gap    3945..4044
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4045..5088
                     /locus_tag="PKL93_09000"
     CDS             <4045..5088
                     /locus_tag="PKL93_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNQ71433.1"
                     /translation="ASTGEGSGSKVASFKYLMIGSVGSLLYLLGIAILYGAAGTLNIS
                     ELAARVSSGQISGDVTLVSSLLIVAGLGVESKLLPFNGWVPDVLTKSSRVATLVLASV
                     YPLAMVNAFSKVLLAVGDDRAMTLVILLGTATVLVGEVLAFSQKSLRRTLAYSSIAQS
                     GLAIFLVGIGSIEAITASIMLLINNALSKFMLFSVDDQVIDELGRDDRNTLNGYGRKS
                     PIVGVTFVVSALSIAGMPLFFGFRGKLNAISASFEASIAVPVIILIAAAIEVTYYFSW
                     IFSFFKPVGTAQTPEKRMVPSVEFVAFGLVLSTVIVFLGVSSGEAFTMFERAADSLVN
                     GVLAIGKAILGGM"
     gene            5089..6921
                     /locus_tag="PKL93_09005"
     CDS             5089..6921
                     /locus_tag="PKL93_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNQ71434.1"
                     /translation="MNILFVIAASLFAAPLFFLVSKFKKSAAYVAYAAFQIFIFVYVF
                     FWGGNGASYEVVGITGRLNFNFSMTPVNWFFASIVMLIISMTAVFMLPLKKSPTKVFL
                     LSLVTSGVIGAVFSADFLTLMIFWEISTWASLILIIQDKEKSTREAIKYAAIAAVGSY
                     SMLFAIFYMDSRLGTVEFSSVALKIESQPIGVQLTIFIALAVMVLAKMGTFPLHTWLR
                     GSHSSAPDEFSPILSGGLTKLGGFLLFTMTIVFPSFKVFGKLPLFNGIPIVNYGFALL
                     GGISIVVGTVMAIRMEDAKELIAFSTVSNSGYIVLALAIGGTYATAGGMMHILNHAMA
                     SAAMFMAIAAVAYRTKTTKMHEMGGLIMKMPVTFAVYLVAIISVAGIPPTSGFVSKWL
                     IYQQLVQDGLPFLAFAAFFGSIGSFMYVFKPLAGIFLGQLKPEHEKVREAPLIMLAPM
                     AFLTFLTVFWGVFPSNAIRSINRITESVGGGTVDVTFSRIVALTGEWDSVLVTTAFAI
                     GFVISLLIFMRAKKARQVDLLDNYTGGDFLYTAELYHFSYRMYRPFDRLFEKWPSMEN
                     WLSSTSEKIKELGALFKAVFFNRNPQVYIALTVIVLLGAFWWLR"
     gene            6926..7834
                     /locus_tag="PKL93_09010"
     CDS             6926..7834
                     /locus_tag="PKL93_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNQ71435.1"
                     /translation="MFMNIVITILSGIALLIVAFVYTVTLEGIARKIDARIQRRYGPP
                     FWQNFIDIFKSLTKHSISHGFIFDFGLLMALGGTIATVFFVPAGSLAVFPGIDNVFVV
                     IYLLAIGLLGMAMSAVGSGNPNASIGIGRALAQMLGYELPFMIVILGVFFHHGTSSLS
                     DLVSIQIAQGAYNAWLMPIGGVVAFISLIGMLGKKPFDTFIAPAEIASGPLVEYSGKY
                     LGMLMIQHAFATFIEIGLFVNLFLGGGRTLWEFLLKFLIVYFSIVIISATIPRFRVEQ
                     AIKFYWKWPLILSFVQVIIVVFVMGR"
     gene            7839..8378
                     /gene="nuoB"
                     /locus_tag="PKL93_09015"
     CDS             7839..8378
                     /gene="nuoB"
                     /locus_tag="PKL93_09015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNQ71436.1"
                     /translation="MSEERKAWEKVANMLRSRSLWMLYYCTGCGAIELPPTMTSRFDM
                     ERLGIGPMATPRQADIMLITGYLSTKTLRRVIYSYEQMQSPKYIVGFGSCTLNGGIYY
                     DSYATINKLDLYVPVDLYLAGCMPRPEAIVSGFTALMSKIDRGEANGWKEYQEKNDWY
                     KKNQIEALGEVYVHDEFHE"
     gene            8362..8877
                     /locus_tag="PKL93_09020"
     CDS             8362..8877
                     /locus_tag="PKL93_09020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNQ71437.1"
                     /translation="MNSMNEILAEVTTIAGELSVKEVTARESIVSVEASMLNAVLELL
                     KRKGFSHLSLITGVDRIKDGVFEVFYTLFRWETGETLLVKSSVSRDKPVITSVMHLWP
                     TARFYERDVHEFFGIVFDGNPDLKPLILENWKEMPPMRKDFDPQKYSNEHFPDRSYET
                     EFLVEGGDENE"
     gene            8870..9976
                     /locus_tag="PKL93_09025"
     CDS             8870..9976
                     /locus_tag="PKL93_09025"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNQ71438.1"
                     /translation="MSKEVKLFLGPNHPGMHGNSSVHLYVEGDTVVRSRLVPGFLHRG
                     FEKLMERRGWMQNLALIPRICVPEPDINEMVYAMAVEALTGTEVPERAQWIRMIILEL
                     ARIASHLMALGGVGGSTGLYTISHWTLADRDRILDIFEKITGARVYHMYIVPGGVRKD
                     LPEGIESEIVEFLDYIESRSDEFENLLLKNRIIRTRTEGLALLTREEALEIGVTGVGL
                     RATGLPYDIRKIDPYAKYDSVAFDIPTATEGDAFARFTLKYYEMMQSIRILRQVLEKM
                     PEGPVNAKISSGSALRWRVPEGTVYTHIESSRGEYGYFVVSDGGERPYRINVRGASFP
                     QGLYGVEKLLAGTRIEDVALWLTTMDFCPPEIDR"
     gene            9989..11818
                     /locus_tag="PKL93_09030"
     CDS             9989..11818
                     /locus_tag="PKL93_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ71439.1"
                     /translation="MSVYDDFKKTFWGPTVAWKRLFTKPVTIKVPQVYREAAERYRGF
                     HVNDWDKCSGCGTCAKICPTDAIKMVPVDITVDIGKKAQRPAIDYGRCSFCGMCVDIC
                     TTGSLNMTREYIHISDDPDSFFFLPDETGIHHDSQPIGYSRDENSELLDLERVEMEEL
                     SGEERVQSFIEFVKGFSKEQAIAEAARCVDCELCIDACPAKMDIPRYIESVFKNDTKK
                     GVEWIYRTNPLPSVCGRVCTHKCETVCSIGNRGEPVAIRWLKRYLMDQEKTEDVIRYA
                     KEQEIVRKGSGRVAIIGSGPAGLSAAYYLSLMGYSVTVYESKEHAGGVMRYGIPRYRL
                     PDEALDADLEVIEALGVKIECGKTVGKDVTIEHLHEKFDAVFLGTGFMKGRSTGVKGA
                     DSEGVLMAMPLLESIRDYLRGDSSKKPEVPESLVVIGGGNVAMDVARSVARLQKMEGM
                     PIDVKVTCLESSDEMPADLEEIVEGREEGIKFFPSRGPKEVCLKGGKVFGLSTIACTA
                     VFDKDGRFNPRFDESDESMIEGKMIVEAIGQAPDYDYLPESIREKIQFVRGRILVNEK
                     GQTDLPWLFAGGDIVNGPDIIHGVADGHKAAVGIDEYLSKGVK"
     gene            11818..12315
                     /locus_tag="PKL93_09035"
     CDS             11818..12315
                     /locus_tag="PKL93_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNQ71440.1"
                     /translation="MSDFKSILEVALSFEKSGREFYKNNMQKVNQSVAKKTFEYLMEM
                     EDSHVKFIEGMIEALEDNKKVDFVLQEEAEEIFVERLESQALSDNSYSSDLADLSILR
                     MAYLIERDFVDYYENASKKVEDTSAKKLMVKLMNWEKGHVTLVKDLMEKIYEKNAIDL
                     GFYPF"
BASE COUNT         3105 a         2604 c         3204 g         3313 t
ORIGIN      
        1 ccgcaaccga gaacttcatg gatctgaaga tgggaccttt catcgcgagt ttcctcttca
       61 tgtcagccat agcgcatttc atagtctcaa cctttggctt tgggtggtac gcaagaaatc
      121 ttcagagagg tattaattac gccagatggt acgagtattc catcagctct tcggtgatga
      181 ttgttgccat cgctatgttc tcgggtatgc ttgacatagt gagccttatg ctgctcttca
      241 ctgtcaacgc tctaatgaat ctctttggac ttatgatgga acttcacaat cagacaaccg
      301 aaagaacaaa ctggacggcc tttatcttcg gctgtatagc cggggcggtc acatggatag
      361 ctgtcttcat atatttgttc acatctcttg gaaaggctaa tattgagttc ccatcttttg
      421 tttatgcaat tctcattact ttcctcgtct tcttcaacag ctttgcgata aacatgttcc
      481 ttcagtacaa gaagatcgga ccctggaagg actacctctt cggcgagagt atgtatgttc
      541 ttctcagcct tgttgccaag agtgtccttg cgtggcaggt tttcgccgga acactcaggc
      601 cagtttgata gaagtttcta aggagtattt gaggccccga aaggggtctc tttgttagga
      661 taattccagg agggtataga tgccaaatct ctacttctat gccggttcaa caaatccgga
      721 gttgtacgag cttcagaata aattgatcga cccggaaagg aaaatagaga aggtcatgga
      781 agagttctgc gatttcgaaa gcggtgtgat tgtagatgtt ggagcaggct caggctttca
      841 ctcacatatg tacgcaagca ctgcctctat ggtttacgcc gtggagccgg attcgggaat
      901 gctgaagcaa ctgatttcaa ggcaagcccg tgacttcaga tctaacttct gtgtggtgaa
      961 gggatttgcc gaggaccttc catttagatc tgatctggca gatatcgtgc actccagact
     1021 tgcgtatttc tttggtccgc ccatgaaata cacagaaagt tgtgaagagg ggattgtgga
     1081 agtcaaaagg gtactgaaga ccgatgggta tttcttcaat attcagaaca attactcttc
     1141 aggccagtat gcggagttcc tcgagctctc atatggtcgg gaccttaaaa accttcaaaa
     1201 agagatttcc gattacttcg tatctagggg ctttttggaa agggttgtga gaacagaatg
     1261 gagagcaaat aaccgtgggg aaatcgaaga ggcccttacg ctagagtttc cgattgagaa
     1321 ggtggggcaa atcatgaaag ccttctccgg caacgagttc tcacacgact tttcaatatt
     1381 cgtcttcagg aaaagaatca gcggcgaaaa tagatagact ctatcttttg ggtcattcga
     1441 gttgcttatt gaattatcat tgacacatag tttcttcccg gatcgatagg gtttcgtctg
     1501 ttcagtaatt ttcggcttat tgtcgagaaa atgatgtgaa attttgaaag atgaaggatt
     1561 gttataccct ataccgggtt atggtatgat actttcattg gtaagtggaa tatttcacaa
     1621 atttcacata aaggggtggt gcaatggccc tgcaattcat ctctctactg gtaatctcct
     1681 ttatcatctg gctgggcttt actgggagtt ttgctcttgc cgagatactt cttgggctcg
     1741 tggtttcgtt tgtcgttgcc attgttttga gcaagtattc aagattccgg atcggtctgg
     1801 attttcctgt tcgcctcttc agattcgtgt tctccttcct tccaatcttc attttcgaaa
     1861 tggttaaggc aaatatcgac gtggccaaaa gagtcttgaa tccttctctt ccgataaacc
     1921 cttcgatggt cgaagttgaa acggatttga aaggggagat ctcgaagctt actcttgcca
     1981 actcaataac tctcacaccg ggaaccttga ccgttgacat tggtgaggac cgtctcttgg
     2041 ttcactggat agacaagaag gccgatgagc cggagagaat aagagaagag atctcgggaa
     2101 agtttgagaa gagaataggg gggatcttcg aatgataggt ttctccatct tcttttcact
     2161 aatactcatt ggcgctggac ttactctcta tagattgttg agagggccga cggttcccga
     2221 cagagtcgca gcactggata tattgaacgt gatgattaca agtgcgatag tgttattttc
     2281 tctaatcgac ggcaatcctc tctttctaga tatagcgctt gtttatgctg cgctctcctt
     2341 tcttgagacc atagtggttg ccaggtatct ggaggggaga agatgatcat attagatatt
     2401 ctggggtatg cgctcttatc tataggcgca tttttcttct tcatgggagg ccttggaatg
     2461 ctgaggatgc cggacgtttt caacaggctg caggccggaa cgaaggcaac tacactgggc
     2521 tcgttctccg tgatactcgg tgtaggactg ataaatccgc agtggctgtt gaagactata
     2581 atcatagtcg ccttcattgc tattacaaat cctgtaggga gttcagcgat cgccagaacg
     2641 gcactgaaga agggaataac accgatagct tatgaggaat ctcataagaa agcagacagt
     2701 ctcccagaag gtggtgagga agagtgaacg tattctcgtt cataatcgga gtagtaatgg
     2761 tgggactggc tattttcgcg attgaagcga agaagctgct gacttccgtg ataatgctct
     2821 cagcaatgag tcttctgtct gtcatacttt ttgttattat gaaggctccg gatgtcgcaa
     2881 taacggaagc ttctgttgga gccggcctta ccacggccat atttctcctc tcccttagga
     2941 agctgaaacg agctgatgta gaatgaagcg gatgatatct gcacttatag cacttctttt
     3001 cttcttcatg atcgtaaccg ttctgaattc cgactcttcc gattctatgg ggattccaag
     3061 atatggtgaa gtgaagctct ccgaaagagt ctctgcgaaa tacatcgaga aatcggtgaa
     3121 tgggaatgaa gatgagatta actttggagt gactgcggac gccgagacgg gttcggctaa
     3181 catggtcact tcgattgtcg tcaattacag gtcattcgac agcctcggag aggtgacggt
     3241 actcttcatt tcagcgaccg gagtcggact gcttcttgga gggggaagaa agagggccgg
     3301 ttcggcaata tccgtcagtc ccgttgtcaa gatcgccgct cgcgtgattg ccccattgct
     3361 tatggtgctg gggatatacg tattcgttca tggccatctg acgccgggcg gagggtttcc
     3421 cggaggagcg ataatcgcgg ccggtttctt gcttctcgtt gcggtcgacg atgaaaagag
     3481 ggcttcaaag ggactgaaga tactggaggg tacaatggga cttctctacg ttctcatagg
     3541 tatagccggc ctgttgatct cgggttcctt ccttaagaat ttccttccca ccggtgtggt
     3601 aggagagctt ttcagcgcag gcattatacc ggttgtttac tcggtaatcg gtatcaaggt
     3661 tggagcagag ctttccggaa ttgtggatga cttcatttcg gaagggggcg atgaggcatg
     3721 atccagtact tttctatgat gctggtcgcc gttggcttat acggccttct ttcgcagaag
     3781 aatctcgtta agctaattat ctctttgaat atagcagaga tcggagtcaa cctcttcatt
     3841 gtctctatag gttacataga agggggagcg gctcctattc tttcttcggt cagcgacaac
     3901 tcgtctcttc tctttgtcga tcctcttccc caggctctag ttctnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnngcatct actggagaag gcagcggttc aaaggttgca
     4081 tctttcaagt atctgatgat aggatcggta ggatcgctat tgtatcttct tgggattgcg
     4141 attctttacg gggcagcggg cactctgaac atttccgaac ttgctgccag ggtatcatcc
     4201 ggccagataa gcggggatgt gacactagtt tcttcacttc ttattgttgc gggtcttggc
     4261 gtcgaatcga aattgctccc cttcaacggt tgggttcccg acgttctgac gaaatcgtcg
     4321 cgtgtggcga cactagttct tgcgtcggtc tatccgctcg caatggtgaa tgcattttca
     4381 aaggtccttc tcgccgttgg agatgatcgc gcgatgactc tcgtcattct tcttggaacg
     4441 gcaactgtcc ttgtaggtga ggtccttgct ttcagtcaga agtcgttgag aagaacgctt
     4501 gcctattcaa gcatcgctca gtcgggccta gcgatctttc ttgtagggat cgggagcatt
     4561 gaggcaatca cggcatcgat aatgcttctt ataaacaacg ccctctctaa gtttatgctc
     4621 ttctccgttg acgaccaggt tatagatgaa ctgggccgag acgatagaaa cacccttaac
     4681 ggttacggcc gaaagtcacc gatagtcgga gtaacattcg ttgtctccgc gctttcaatc
     4741 gcaggtatgc cactcttctt cggattcagg ggaaagctga acgcgatctc ggccagtttc
     4801 gaggcctcta tagccgttcc tgtaataatt ctcattgcag cggccattga agttacatat
     4861 tacttcagtt ggatcttctc cttcttcaag ccggttggaa cagctcagac gcctgagaag
     4921 agaatggttc catcggtgga gttcgttgcc ttcgggcttg tgctaagtac ggtcattgtc
     4981 ttcctcgggg taagctccgg ggaggctttc acaatgtttg agagagccgc ggattctctt
     5041 gtaaatggcg ttctggcaat cggaaaggcc attctgggag ggatgtagat gaatatactc
     5101 tttgtgatag cggcatctct ctttgccgct ccgttgttct ttctggtttc gaaatttaag
     5161 aagagtgctg cctatgttgc gtacgccgcg ttccagatat ttatctttgt ctatgtattt
     5221 ttctggggag gtaacggagc atcatatgaa gtggttggga taactggaag actcaacttc
     5281 aatttctcta tgacccccgt taactggttt ttcgcttcga tagtgatgct aataatatcc
     5341 atgactgcgg tcttcatgct tcctctaaag aaaagcccca ccaaggtctt tctgcttagt
     5401 cttgtaactt caggagtgat cggtgcggtc ttctcggccg atttccttac tttgatgatc
     5461 ttctgggaga tttccacatg ggcctctctg attctcataa ttcaagacaa ggaaaagtcg
     5521 actcgagagg cgataaagta tgccgctatc gcggcggtgg gaagttattc aatgttgttc
     5581 gcgatctttt atatggacag caggctggga accgtcgaat tttccagtgt ggctttgaag
     5641 atcgagagcc aaccgatcgg tgttcagctc acaatcttca tagccttggc ggtaatggtt
     5701 cttgcaaaga tgggaacttt ccctctccat acatggctta ggggctcaca ttcttctgca
     5761 ccagacgaat tcagccccat cctttccgga ggactcacaa aactcggtgg ctttctgctc
     5821 ttcaccatga cgattgtttt cccctccttt aaggttttcg gcaaacttcc cctatttaac
     5881 ggcattccga tcgtaaatta tggatttgcc ctccttgggg gcatttcgat agtcgtggga
     5941 acggttatgg ctatacgaat ggaagacgcc aaagaactca tcgccttttc gactgtaagc
     6001 aattcaggct atatagtgtt ggccctcgcc attggtggaa cctacgcgac tgccggagga
     6061 atgatgcaca ttctcaacca cgcaatggca tctgccgcca tgtttatggc gatagctgcg
     6121 gtagcctaca ggacaaagac tacgaaaatg cacgagatgg gaggactcat aatgaagatg
     6181 cccgtcacct tcgccgttta tctcgttgcg attatttcgg tggctggaat tcctcctaca
     6241 agcggattcg tgtcgaagtg gttgatctat cagcagctag tgcaggatgg tttgccgttt
     6301 ctagcctttg ccgctttctt tggaagcata gggtcattca tgtatgtctt caaacctcta
     6361 gcggggattt tcctgggaca actgaagccc gaacatgaga aagttagaga agcaccgctg
     6421 attatgcttg ctccgatggc ctttctgaca tttcttactg ttttctgggg agtctttccg
     6481 agcaacgcta taagatcgat caacaggatt acggaatcgg ttggcggagg aactgtcgac
     6541 gtgactttct cgagaattgt cgcacttact ggggaatggg actcagtttt ggttacgact
     6601 gccttcgcga taggcttcgt gatatcgttg ttgatcttca tgcgcgcaaa gaaggccaga
     6661 caggtagatc ttctagacaa ttacacgggc ggggattttc tgtacaccgc agagctttat
     6721 cacttttctt acaggatgta caggccgttc gacaggttat tcgaaaagtg gccttcgatg
     6781 gaaaactggc tttcatcgac ttctgagaaa atcaaggaac ttggagccct gttcaaggca
     6841 gtctttttca accgtaatcc tcaggtatac attgctttga ctgttattgt tcttcttggc
     6901 gccttctggt ggttgaggtg atgacatgtt tatgaatatc gtaatcacaa tactttcggg
     6961 gatcgcgctt ttaatagtcg cgtttgtata taccgtaacc cttgaaggaa ttgcaagaaa
     7021 gatagatgca agaattcaga gaagatacgg cccgcctttc tggcagaact tcatagatat
     7081 tttcaagtct ctgacgaagc actcgatttc ccacggcttc atcttcgact ttggtttact
     7141 tatggctctg ggagggacga ttgcgacggt cttcttcgtc ccggccggca gtctcgccgt
     7201 ttttccggga atcgacaatg tctttgtagt gatctatctt ctggcaattg gccttcttgg
     7261 aatggcgatg tctgccgtag ggtcgggaaa tccaaatgca tccataggaa tcggaagggc
     7321 actggcccag atgcttggat acgaacttcc cttcatgata gtgattctcg gggtcttttt
     7381 ccaccatggg acttcatctc tatccgatct tgtaagcatc cagatagcgc agggcgctta
     7441 caacgcttgg ttaatgccga ttggcggagt agttgccttt atttctctga tagggatgct
     7501 cggaaagaag cccttcgaca ccttcattgc accggccgag atagcttccg gaccacttgt
     7561 ggagtacagc gggaagtatc ttggtatgct gatgatccag cacgcatttg cgacattcat
     7621 tgagatcgga ttgtttgtga acctcttcct tggaggcggt aggactttat gggaattcct
     7681 cttgaagttc cttatagtgt acttctccat agtaatcatc tccgccacta ttccaagatt
     7741 cagggtcgag caggcgatca aattctactg gaaatggccg ctcattctct ccttcgttca
     7801 agtaatcata gtggtctttg tgatggggag gtgatggcat gagcgaagaa cgcaaagcat
     7861 gggaaaaagt agcaaatatg ctcagatcac gctcgttgtg gatgctttat tactgtaccg
     7921 gatgtggagc tatagagctg cctccaacga tgacttcgcg cttcgatatg gagcgtctcg
     7981 ggattgggcc tatggcaact cctaggcagg ccgacattat gttaatcaca ggatacctaa
     8041 gtacgaagac tctcaggaga gtcatttatt cgtacgaaca gatgcagtcg ccgaagtata
     8101 tagtcggctt tggttcttgc actttaaatg ggggaatata ctatgactcg tacgcgacga
     8161 taaacaaact ggatctttat gtgcctgtgg atctttatct ggccggttgt atgccccgcc
     8221 cggaagctat agtaagcgga ttcaccgcgc tgatgagcaa gattgacaga ggagaggcca
     8281 acggctggaa agagtatcag gagaagaacg attggtacaa gaagaatcag atagaggctc
     8341 tcggggaggt gtatgtacac gatgaattcc atgaatgaga tacttgcaga agtgacgact
     8401 attgccggag aattgtcggt caaggaagta accgcccgtg agtcgatcgt atccgttgaa
     8461 gcttcaatgc tgaacgcggt tcttgagctc ttgaagagaa aaggcttctc tcatctctca
     8521 ttaataaccg gcgtcgacag gataaaagac ggagtcttcg aggtgttcta cacccttttc
     8581 agatgggaga cgggagagac tctgttggtg aagagttctg ttagtagaga caagcccgtc
     8641 attactagcg tgatgcacct ttggcctaca gcaaggtttt atgaaagaga cgttcacgag
     8701 ttctttggta tagtgttcga tggcaacccc gatctcaaac cactcatact cgaaaactgg
     8761 aaggaaatgc caccgatgag aaaagacttc gatccgcaga aatattcaaa cgagcacttt
     8821 ccggacagga gttacgaaac agagttcctg gtcgaagggg gcgatgagaa tgagtaagga
     8881 agtcaagctg ttcttgggcc ctaaccaccc cgggatgcat ggcaattcaa gcgttcacct
     8941 ctacgtggag ggtgacaccg tcgtcagatc gagactcgtt cctggctttc ttcacagggg
     9001 cttcgagaag ttgatggaga gaagaggctg gatgcaaaac ctggccttga tcccccgaat
     9061 ctgcgtgccc gaaccggaca taaacgaaat ggtttacgca atggcagtcg aagcgctcac
     9121 cgggacggaa gtgccagaaa gggctcaatg gataaggatg ataattctcg agctggcgag
     9181 aatagcttcc catctaatgg ctttgggtgg agtcggagga tctacgggtc tctacacgat
     9241 ttcgcactgg actcttgccg acagggacag gattctagat attttcgaga aaattacagg
     9301 cgcaagggtc tatcacatgt atatcgttcc tgggggtgta agaaaggatc tgcccgaagg
     9361 gatagaatct gagatagttg aattcctgga ttacatagaa agcaggagcg atgaatttga
     9421 gaacctgctc ctgaaaaaca ggataatcag aacgaggaca gagggtctcg cgcttctaac
     9481 cagagaagag gcgctggaaa taggtgtaac cggcgtggga ctgagagcaa caggtctgcc
     9541 ctatgacata agaaagatag atccgtatgc aaaatatgac agtgtggctt tcgacattcc
     9601 caccgcaact gaaggggatg catttgccag attcactttg aaatactatg agatgatgca
     9661 gagtatcagg atactgaggc aggtacttga gaaaatgccg gagggacctg tgaacgcaaa
     9721 gatctcatct ggcagcgctc tcagatggag ggtgccggag ggaactgtct acactcacat
     9781 agagtcatcc aggggcgagt acggatactt tgttgtctca gatggagggg aaagacccta
     9841 caggataaac gtcagagggg cttcctttcc tcagggttta tacggtgtcg agaaactgct
     9901 agcaggcaca agaatagaag acgttgctct ctggctaacc actatggact tctgtccgcc
     9961 agagatcgac agataggggg ctggtaacgt gagcgtatac gatgatttca aaaagacttt
    10021 ctggggtcct actgtagcct ggaagagact gttcacgaag ccggttacaa taaaagttcc
    10081 acaggtttat cgcgaagcag ccgaaagata cagggggttc cacgttaacg attgggacaa
    10141 atgctccgga tgtggaactt gcgcaaagat atgccccacc gacgccatta agatggtgcc
    10201 agtggatatt acggtcgata tcggaaagaa ggcacagcga ccggctatag actatggaag
    10261 atgttcgttc tgcggaatgt gtgtcgacat atgtacgacg ggttcactga acatgaccag
    10321 ggagtatatt catatatccg acgaccctga tagttttttc ttcctaccag atgaaactgg
    10381 catccatcat gatagtcaac ctataggcta ttcacgcgac gaaaactcgg aactgctcga
    10441 tcttgaaagg gtagaaatgg aagagctctc gggtgaagag agagttcagt cgtttataga
    10501 atttgtcaag ggattctcga aagagcaggc catagcggag gcggcaagat gtgtggactg
    10561 cgaactctgc atagatgcct gcccggcaaa aatggatatt cctagataca tagagagcgt
    10621 cttcaagaac gatacaaaaa agggcgtcga atggatatac aggaccaatc ctttgccgtc
    10681 ggtttgtgga cgcgtctgta cccacaagtg tgagaccgtc tgttcaatag gtaacagggg
    10741 agagcccgtt gccataagat ggctgaaaag atatctcatg gatcaggaga agacggaaga
    10801 cgttataaga tatgcgaaag aacaagagat agtcagaaag ggaagtggca gagttgcgat
    10861 aataggcagt ggcccggccg gtctgtctgc cgcatattac ctttcgctaa tgggctactc
    10921 tgttacagtc tatgaaagca aagagcatgc gggcggtgtc atgaggtatg gtataccacg
    10981 ctatcgtctc cccgatgaag cattggatgc agacttagaa gtaatagaag ccttgggtgt
    11041 gaagatcgaa tgcggcaaga cagttggaaa ggacgtcact atagaacacc ttcatgagaa
    11101 gttcgatgcg gtctttctcg gaacggggtt catgaaggga agaagcaccg gagtcaaagg
    11161 agctgacagc gaaggggttt tgatggccat gcctcttctc gagtctattc gcgattatct
    11221 cagaggggat tcaagcaaga aaccggaggt gccagaaagc cttgtcgtaa taggcggagg
    11281 caacgttgca atggacgtgg ctcgtagcgt tgcccgtctc cagaagatgg aaggaatgcc
    11341 gatagatgtc aaagtaacat gtctcgaatc ctcggatgag atgccagccg accttgagga
    11401 gatagtagag ggaagggaag agggaatcaa attctttcct agtcgaggac cgaaggaagt
    11461 ctgtctcaag ggcggcaaag tcttcggatt atctacgatt gcttgcacag cagtctttga
    11521 taaagacgga agattcaacc caagattcga tgaaagcgac gaaagtatga tcgaaggaaa
    11581 aatgattgtt gaggcaatag gtcaggcgcc ggactacgat tatcttccag aatcgatcag
    11641 agagaagata cagttcgtaa ggggaagaat actcgttaac gagaaaggtc aaacagatct
    11701 tccctggctt ttcgcgggag gagatatcgt gaatggtccg gacatcattc atggcgttgc
    11761 cgacggtcac aaagcggcag ttggtatcga cgagtacctt agtaaagggg tgaagtgatg
    11821 agcgatttca agagcattct ggaggtagca ctcagtttcg aaaaatcagg ccgggagttc
    11881 tataaaaata atatgcagaa ggtcaatcaa tccgttgcca aaaagacctt cgaatatctt
    11941 atggaaatgg aagactctca tgtgaagttc attgagggta tgatcgaagc tcttgaagat
    12001 aacaagaagg ttgattttgt cctacaggaa gaagcggagg agatattcgt agagagactg
    12061 gaatcgcagg cgctctcaga caactcttac agcagcgatc ttgccgatct ttcgatttta
    12121 agaatggcct acctcattga gagggatttt gttgattact acgaaaatgc ctccaaaaag
    12181 gtggaagaca cgagtgcaaa gaaactcatg gtaaagctaa tgaactggga aaaagggcat
    12241 gttactcttg tcaaagacct catggaaaag atatacgaaa agaacgcaat tgatcttggc
    12301 ttctatccct tttgattgga gagaac
//
LOCUS       DAOQYI010000043        12078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_5749_length_12078_cov_79.530483, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000043 DAOQYI010000000
VERSION     DAOQYI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 12078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12078
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_12078_cov_79.530483"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1810
                     /locus_tag="PKL93_09040"
     CDS             182..1810
                     /locus_tag="PKL93_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71441.1"
                     /translation="MRRLFLVGILLILLTSSFAEVLKRRGTEEEFSYSELAGGSITLP
                     VDTTFRFIPGFELSEGDFWVTFAIQDPLFRWASGDELSPCLVEKYELLNSNKSLIIKL
                     RDDIFWYDGTPITSADIEYSIKAWKSTESSPVYEALNDPRFGWFSVVDGKTVVFNFND
                     SYPEFLNSLRTGILAKHIYSDRLGIEPESLEENMEMDKPPVEATSGSFMLDPASVKGN
                     IILKRNPNWHGGSGDDLFPLVFESWPGKSLLDEIRLVEVAEPLDRIEMFDAGELHLLF
                     SEPDHNESLLSEAASGKFKSGSFPDGSYHVILINHRNALLAERGVRQALKMSLDYESL
                     LEALPNSIGTFIPVDPRTSIFKTLQAQIVDLPYDPDKAKTFLSNAGYPGSITLSIKVY
                     HGVENILLEELQKSWDKIGVKLEVERLDWGSLLRDIDEGDYELVYLRVEAFDYPEIAP
                     WTNEYEHDLKTGWEVGYVNPQIFRIMRRAAIETDWRKRTPYYLGSYRIWTNDVPVLVL
                     AYNLSYAFWNSQLSGPVPGRGELYENLAEWYLQK"
     gene            1838..2758
                     /locus_tag="PKL93_09045"
     CDS             1838..2758
                     /locus_tag="PKL93_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71442.1"
                     /translation="MLYLEKEPFRRAQYTILENGRELEKRLFAYYFMDGPANIVVEEI
                     ASYQNVDGGFGNAIEPDFRLPASSPMATSVALQHLVHFDDLPLAKGVISKAIAYLDNT
                     YVSERKGWFAVPRTVNDYPHAPWWGCDEEKEICAIDANWGNPTAEIIGYLTRYREFTN
                     LDVDELISQATRYLESMNTFTSFHEILCFVRLYRLLDEKTAASLRPKLYEAVCTLISK
                     DEEEWRTEYVAKPLDFVTSPDGFFCVDRELIEKNIEYYINELQTHGKIKPTWSKSFYD
                     GEMSRAWDEWVAIITLRVLLILKSFGRILL"
     gene            2784..3392
                     /locus_tag="PKL93_09050"
     CDS             2784..3392
                     /locus_tag="PKL93_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNQ71443.1"
                     /translation="MKNIILQERIMAVIRSDSVERCVEIGRSCFRGGVKILEITFTVP
                     DAPKAIAILSKEIEGAYVGAGTVITDDQFSKAVKSGAKYVVSPHLSEELAIQSRALET
                     LYLPGIMTPTEYVKATALGSSMVKVFPGDVLSPSFVKSLRGPFPEALCVVSGGVNLDN
                     IDTWFKAGTAAVGIGSDLTKGSPEEIEEKARQYVAKVKNFFG"
     gene            complement(3399..4808)
                     /locus_tag="PKL93_09055"
     CDS             complement(3399..4808)
                     /locus_tag="PKL93_09055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HNQ71444.1"
                     /translation="MIGFLAIDIGASSGKAFVGKYGRNRLVLEEVHRFSNEPIHVADL
                     THWNLISLYKNVVESIKAASAMGIEIRSVGIDTWGVDFGLIDSSGSIQGDPRHYRDMF
                     KDDSMDYAIKKAGKRWIFDRAPTQFQPFNTLYQLISLKRKNSRYLEKADALLPMPSLL
                     TYFLTGEKLTEFTFATTTQLFDPDRNDWSEELIRFFDLPDIMTPIVDPGEIAGELLPS
                     VAEELGIKGLKVILPASHDTASAVSSMDLSDDSMFVSAGTWCLEGVLIDKPSRNDDVI
                     ENNLANEGCYGRRYRLLKNLTGLWLVQELLREWRRSDPNLDHYALTEMALTARSYERK
                     IDVESETFRKPRSMEKAILSEAEKLGISLKNRGEIVRAAIEGIAYQTEQTRKQLQSIT
                     GRSIRNIRMVGGGIRNSLLCQLVSDYTGLPVVAGPAEGTATGNIIVQMLGLGELSDLS
                     QAHDLIQRSFNFQEYTPKK"
     gene            complement(4805..5794)
                     /locus_tag="PKL93_09060"
     CDS             complement(4805..5794)
                     /locus_tag="PKL93_09060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HNQ71445.1"
                     /translation="MRDRSKPVLCSGEILFDFISREPMKGLEGTTLFEKKPGGAPFNI
                     SVGLHRLGVPMEFLAKIGMDEFGNALLEYLKSLGISTSYVIREHGTKTPIAFAALDGE
                     GKPEFRFYWDHAAHLSLKSEEIEEIDPMNFSLFHFGAIVLLEEPSASTYLELFERFSS
                     RGVKTSFDPNIRRSAIEDHDSFRATVREIISRVDILKLSDDDLLYICDSDNLDEALSR
                     LKVRPDTLVFLTLGSRGSTVYRGRTSVKREGFKVKVAETTGCGDSFMAAVISKLYNMG
                     DEELRSLTEEDLGSILTFANAEAAIVATRYGGASSMPAESEVNQFLKERGMDH"
     gene            complement(5791..6438)
                     /locus_tag="PKL93_09065"
     CDS             complement(5791..6438)
                     /locus_tag="PKL93_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNQ71446.1"
                     /translation="MLYIDGICEKSVELVEQPVFAGITTNPTILKRDRPGWGLMDAMK
                     FLSKVPGERHFVQGSLCSTEWIQKVKEFIKKSDFDPEFFTIKLPWDPQKASNIVPQLN
                     DIGVGVCATAVYTLQQYYAAVSMEVEYVAVYFDRMVKAGIDADLRITEMLDIGDWHSN
                     APRIIAASIKDIESANKLISLGVHDLTLPIEIAAEYVQGSFPADDLNRFEADFKL"
     gene            complement(6482..7900)
                     /locus_tag="PKL93_09070"
     CDS             complement(6482..7900)
                     /locus_tag="PKL93_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HNQ71447.1"
                     /translation="MKKRKVGVITFSDGRDFVHNDLIEMNKGFQDRLVKALEATGEVE
                     VVTASDIVWKPSLAKKAGKELMKAEVEATIFNYAIWCWPHLSVMASLYAPGPFLTYGQ
                     INPSYPGMVGQLAAAGALEQVGIMPERVWGNPEDSDVIERVMHFVRAASAAARLKGER
                     YGMFGGRPMGMYTASANGDQWLKEFGVDVEQIDQYELVVRAEKVPKDVTKAAREWLEK
                     LATVKYDGKQLTPEKLEKQIALYEAALSIIKEYELDFVGFKGQPEMTNNYATMDIVEA
                     FLNDPYDWHGSKETIVAATETDMDGALTMEIFKHIAQSPVLFADVRHYFEEEDLLDLC
                     NSGTHATYFAGKSMNPEDNLKHVELYPEDFYFPAGGAAVKHFAAPGRVTLARLARQNG
                     EYIMTIVPGEFVKLSEAEEKKLSEKVQIEWPHAYVKLDTDMDTFLRYYPCNHTHGVYG
                     DFVEELVQFCDIKGIDYQILGE"
     gene            complement(7941..8966)
                     /locus_tag="PKL93_09075"
     CDS             complement(7941..8966)
                     /locus_tag="PKL93_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ71448.1"
                     /translation="MKKTTIKDIANELNISPSSVSRALSDEPGVSPSLRKEIVDAAER
                     LNYIPNSSAKSLKTRKTQTVGLIVSDIRNPFFLDFMSGVETVLFPRGYKFIVCDIGED
                     FEKERIYIKWLLEHGVEGILSSPCGGEDGRNNSRLYREAVRRNIPVVFYDRILEGLDR
                     ISSVTLDNRQAILQGVLHLKENGHRKLGIVLHKKGIYTIEERRMGFLEACKMLDIEIN
                     ADWIIENAVDAERASLSLESVLKSDRRPDAIISTNQFLNEVIVATVRRMGLKIPGDLS
                     IVGFDDHSMNELIEPPLTTIRQPVKDIGRIAATIMLSEIDGTRGDKSKVVLKAELLQR
                     KSVSKLI"
     gene            complement(9086..9985)
                     /locus_tag="PKL93_09080"
     CDS             complement(9086..9985)
                     /locus_tag="PKL93_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ71449.1"
                     /translation="MPAVDIAIDVGGTKTLVSAFDEKEVEPYSSEEFPTRPAEGIDSF
                     FERLSNICLRMKGSRNINRWGLCTAGAVSPEKGKLIWSPNLGWKDVELFKRASSFLGT
                     EGVIENDCNAAAFGEWEFRKDIESLIYVTVSTGIGMGMVVRGKILRGASNAAGEIGHT
                     IVKADGPLCTCGRRGCLQAISGGRGLEIRAHDSLGIEIGTKEITDRAELNEPVFKEMI
                     TEASETLGRFLCNLIDSLDPSVLVIGGSLGKNRYYFDRILKTIEENYYRVPGKKVKIE
                     LSSIEPNPNILGILSLSRKTHKD"
     gene            complement(9972..10598)
                     /locus_tag="PKL93_09085"
     CDS             complement(9972..10598)
                     /locus_tag="PKL93_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNQ71450.1"
                     /translation="MFERLMTEKVLTVIRGLTLDQSRKLCEMLLENDLTILEVSFSDE
                     SSSEILHELKKEFYGKLVIGAGTVYDDTAYSRALRSNADFVLSPGFSEEIAKLSTRDG
                     ISYIPGVYTSTDIQTALNNGFSFLKLFPGDAEGLNLLKAYRGPFPTVKFMPFGGVTAE
                     NATEYMKAGAVALGIGSYIANRNLFDEEREGEVLERILEIRRAVNASS"
     gene            complement(11345..11554)
                     /locus_tag="PKL93_09090"
     CDS             complement(11345..11554)
                     /locus_tag="PKL93_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71451.1"
                     /translation="MAFKDYHIISLIMTIVFVTFYGTLLAGIGDSEESNIPIIKQNQL
                     RTTLEGPTCGESFDRLYMALLFIRS"
     gene            11866..>12078
                     /locus_tag="PKL93_09095"
     CDS             11866..>12078
                     /locus_tag="PKL93_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71452.1"
                     /translation="MESVYKKLRIYNLIMGFLHLVQGIIMLIVSNDSSLTITRNYLEF
                     NQEIMRLVPVSSPGGFHPQALSEPDLN"
BASE COUNT         2959 a         2911 c         2828 g         3380 t
ORIGIN      
        1 tctatcctat ctgttcttct atttatctcc aatatccttg cggggtaaat atgctctatt
       61 cagtgaacgg tgaaccattc aacgaagaac cattttctcc tgctctttgt ctttccgaac
      121 gaaatcgaat taggtttatc taatataata gtggagagac ttgaagcttt ggaggtaccc
      181 tgtgcggaga ttatttctag tcggtatatt gctaatcttg ttgaccagta gtttcgccga
      241 ggttcttaaa agaagaggta cggaagaaga attctcttac tcggagttag ccggaggttc
      301 gattacactg ccggtcgata ctacgttcag attcattcca ggctttgagc tttcggaggg
      361 tgatttctgg gttacttttg cgatccagga tccgcttttc agatgggcaa gcggcgacga
      421 attgagtcct tgcctggtgg aaaagtacga gctgttgaat tcgaacaagt cacttatcat
      481 aaagctcaga gacgatattt tctggtatga cggaactcct atcactagcg cggatattga
      541 gtactcgata aaagcctgga agagcaccga aagctctccg gtctatgagg ctctcaatga
      601 tccgcggttt ggctggtttt ccgtcgtcga cggcaagacc gtggttttca atttcaatga
      661 ttcgtatccc gaattcttga actctttgag aacgggcata ctcgccaaac atatttactc
      721 cgacagactt ggaatcgaac cggagagcct ggaagagaac atggagatgg ataaaccacc
      781 cgtggaagct acatcagggt cgttcatgct cgatccggca agcgtgaaag gcaacattat
      841 tctaaagaga aacccgaact ggcacggagg atctggagac gatctctttc cgctagtatt
      901 cgagagttgg cctggaaaga gccttctaga tgaaataagg ctcgtggaag tggcagagcc
      961 tcttgacaga attgagatgt tcgatgcggg cgagctgcat cttctcttca gtgaaccgga
     1021 ccataacgag tctcttctta gtgaagcggc ttccggaaag ttcaaatcgg gaagcttccc
     1081 ggatggtagt tatcacgtta tcctcataaa tcatagaaac gcgctcctgg cagaaagggg
     1141 agtgagacag gcactcaaga tgtctcttga ctacgaatct cttcttgaag ccctccccaa
     1201 ctccattggg actttcatcc ctgtggatcc caggacgtcc atttttaaga ctttgcaagc
     1261 tcagatagtg gatcttccgt acgatccgga taaggcaaag acctttcttt caaatgcagg
     1321 ttatccgggt agcataactt tgtcgatcaa ggtctatcat ggagttgaga atatattgct
     1381 ggaggagctc cagaagagct gggacaagat aggtgtgaaa ctcgaagttg aaaggctcga
     1441 ttggggaagc cttcttcgtg acatagacga aggtgattac gagctggtct atcttcgagt
     1501 cgaggccttt gattatcccg agatagcccc atggaccaat gagtatgagc atgatttgaa
     1561 gacaggctgg gaagtgggtt atgtaaatcc acagattttc aggattatga gaagggccgc
     1621 cattgaaact gactggcgaa aacgtactcc ctattatctt gggtcataca ggatatggac
     1681 gaatgatgta ccggttctag ttcttgccta caatctctct tacgctttct ggaatagcca
     1741 gctcagcggg ccggtcccgg gcagagggga gctttacgag aatcttgctg aatggtattt
     1801 acagaaatga aaggacaaca taaagggggt attaaaaatg ctctatcttg aaaaggagcc
     1861 ttttaggaga gcacaatata cgatccttga gaatggtaga gaactggaaa agagactatt
     1921 cgcctattat ttcatggatg gcccggcaaa cattgttgtt gaggagatag ccagttatca
     1981 gaatgtcgat ggcgggtttg gaaacgctat agagccggac ttcagattgc cagcgtcttc
     2041 tccaatggca acgtccgtag cgcttcaaca ccttgttcac ttcgatgatt tgccacttgc
     2101 aaagggagta atctcgaaag cgattgccta tttggacaac acatatgttt cggaaagaaa
     2161 gggatggttt gccgttccac gaaccgtcaa tgactacccc cacgctcctt ggtggggatg
     2221 cgatgaagag aaggaaattt gcgcaatcga tgcaaactgg gggaacccaa cggccgagat
     2281 cataggatat ttaaccaggt acagggagtt caccaatcta gatgtagatg agcttatttc
     2341 tcaggcaacc aggtatctgg aaagtatgaa cacgtttacc tcttttcacg agatattatg
     2401 ctttgtaagg ctctacagat tacttgacga aaagacggcc gcaagtttaa ggccaaagct
     2461 ctatgaagca gtctgcacat tgatcagcaa ggatgaggag gaatggcgaa cggagtatgt
     2521 agccaagccg ctggattttg ttacctctcc cgatggattt ttctgcgtcg atagagagct
     2581 gattgaaaag aatatcgaat actatattaa cgagcttcag acgcacggta agatcaagcc
     2641 cacttggtcc aagagcttct acgatggcga gatgagcagg gcctgggatg agtgggttgc
     2701 tataataacc ttgagagttc ttttgattct caaatcattt ggaagaatat tactctaaat
     2761 agaattcgaa tggggggata gaagtgaaga atattatcct gcaggaaagg ataatggctg
     2821 tcatcagaag cgacagcgtg gagagatgtg tcgagattgg aagaagctgt ttcagaggag
     2881 gcgtgaagat cctggaaata accttcacgg ttccagacgc ccccaaggcg attgcaattc
     2941 tctcaaaaga aatagagggt gcctacgttg gagctggaac ggttatcact gacgaccagt
     3001 tcagcaaggc agttaagagc ggcgcaaagt acgttgtaag tccgcatctt tccgaagagc
     3061 ttgcgattca gtccagagcg cttgagacgc tatatcttcc cggaataatg acgccgacgg
     3121 agtatgtaaa ggcaactgcg ctgggcagct cgatggtcaa agtcttcccc ggcgatgtcc
     3181 tttcgccatc ctttgtgaag tcacttaggg gtcccttccc cgaggcgctg tgtgtggtgt
     3241 cgggaggagt caatcttgac aacatagaca cgtggttcaa ggcaggcacc gccgccgtcg
     3301 gtataggatc cgatctgact aaggggagcc cggaagaaat agaggagaag gcaagacagt
     3361 acgtcgccaa agtcaagaat ttctttggat aaatcttgtt actttttcgg agtgtattcc
     3421 tgaaagttga atgagcgttg aataagatcg tgagcctgcg ataggtcgct tagttctcca
     3481 agaccaagca tctgcactat gatgttaccc gtggcagttc cttccgccgg tcctgcgact
     3541 accggaagcc ctgtgtagtc tgacactaac tggcagagaa ggctgttgcg aatgccaccg
     3601 cccaccattc taatgttcct tattgaccgg ccggttattg actgcagttg ttttcttgtc
     3661 tgctctgtct ggtaagcgat tccttcgata gcggctctca cgatctctcc cctatttttc
     3721 aaagagattc cgagcttctc ggcttcggag agtatagcct tctccatgct tctcggtttt
     3781 ctgaaggttt cggattccac atctattttt ctctcatagc ttctggcggt cagcgccatc
     3841 tcggtcaacg catagtgatc gagattcgga tccgatctcc tccactccct gaggagttcc
     3901 tgaaccaacc atagaccggt tagattcttg agcaggcggt accttcttcc gtagcagccc
     3961 tcgttggcaa ggttgttttc gataacgtcg tcatttctgg aaggtttgtc gatcaacacc
     4021 ccctctaggc accaggttcc ggcgctgacg aacatcgaat catctgaaag atccatggac
     4081 gaaacggccg acgccgtgtc gtggctcgct gggagaataa ccttcaagcc ctttatgcca
     4141 agctcttccg cgactgaggg gagcagttct ccggcgattt cccctggatc tacgatcgga
     4201 gtcattatgt ccggtagatc gaagaatctt ataagctctt cgctccagtc gtttcgatcg
     4261 ggatcgaaga gttgtgtggt agtggcgaaa gtgaactctg tcagcttctc ccctgtgagg
     4321 aagtaggtca ggagtgaagg catcgggaga agggcatctg ccttttcaag gtaacggctg
     4381 tttttacgtt tgagagatat gagctgatag agagtgttga atggctgaaa ctgtgttgga
     4441 gctctgtcga agatccatcg cttgccggcc ttctttattg cgtaatccat ggaatcatct
     4501 ttgaacatgt ctctgtaatg tctgggatcg ccctgaatcg aaccggaact gtcgatgaga
     4561 ccgaaatcca ctccccatgt gtcgattcct acgctccgga tctcaattcc cattgctgaa
     4621 gccgccttta tactttccac gacattcttg taaagcgaaa tgaggttcca gtgagtcaaa
     4681 tccgcgacat gaataggctc gttcgaaaag cgatgtactt cttccagaac cagcctgttt
     4741 cgaccgtact ttccaacgaa ggccttgccg ctggaggcac cgatgtctat ggcgaggaat
     4801 ccgatcaatg gtccataccc cgttctttaa ggaactggtt cacttcgctt tcggctggca
     4861 tcgagcttgc tccgccatat cttgtcgcga caatcgccgc ctctgcgttt gcgaaagtga
     4921 gaatgcttcc cagatcttct tcggtaagac tacgcagttc ttcatcgccc atattgtaga
     4981 gcttagagat tactgcggcc atgaaagagt ctccgcagcc ggttgtctcg gccactttca
     5041 ccttgaaccc ctctctctta acagaagtcc ttccgcgata aacagtggat cctctgctgc
     5101 cgagggtgag aaagacgagc gtgtccggtc tgactttgag ccgagacagg gcctcatcta
     5161 aattgtcgct gtcacatatg tatagcagat cgtcgtcgct gagcttgaga atgtcgactc
     5221 ttgagatgat ttctctgact gtggccctga atgaatcgtg atcttctatt gcgcttcttc
     5281 gaatgttggg gtcgaatgaa gtcttgactc ctcttgaact gaacctttcg aacagctcta
     5341 gataagtgga tgccgacggt tcttctagga gtactatcgc gccaaaatga aatagggaaa
     5401 agttcatggg gtcgatctct tcgatttctt ctgatttgag cgatagatgg gctgcatgat
     5461 cccagtagaa tctaaattcc ggctttccct ctccgtccag agcggcaaag gctatcggtg
     5521 tcttcgttcc atgttctcta atcacgtagc tggtagatat tccaagagac ttcagatact
     5581 caaggagtgc gtttccaaat tcgtccatgc cgattttggc gaggaattcc atagggactc
     5641 ccagtctgtg aagaccgaca gatatgttga aaggtgcacc acccggtttc ttttcaaaca
     5701 gggtggtgcc ttccaggcct ttcataggtt ctctagagat aaaatcgaag aggatctctc
     5761 cagagcataa aaccggtttg gaccgatctc tcataacttg aagtcagcct cgaacctgtt
     5821 gagatcatct gccggaaaac taccctgaac gtattcagct gcgatctcga tcggcaatgt
     5881 gagatcatgg acacccagcg agataagttt gtttgcgctc tcgatatcct ttatacttgc
     5941 cgcaatgatt ctaggggcgt ttgagtgcca gtcaccaatg tctaacatct ccgtaattct
     6001 caagtcagca tctattcccg ccttcaccat tctatcgaag tacacggcca catactcaac
     6061 ttccatgctg accgcggcgt agtactgctg taaagtgtaa acggccgtag cacatactcc
     6121 aactccaata tcgttcagct gagggacaat attagaggcc ttctgcggat cccagggcag
     6181 tttgatagta aagaattcgg gatcgaaatc gctcttcttt atgaattcct tcaccttctg
     6241 aatccattcg gtcgaacaga ggctaccctg aacgaagtgt ctctcgcccg ggacctttga
     6301 gagaaatttc attgcatcca tcaggcccca tcccggccta tctcttttca gtattgtagg
     6361 gttagtggtg attccggcaa agacaggctg ttcaacgagt tcaacgctct tttcacaaat
     6421 accgtcaatg tataacatta tcaggctcct ttcttagtaa ctatatgtcc cgaaatgcag
     6481 tttactctcc aagaatctgg taatcaatac cctttatatc acagaactgt accaattcct
     6541 ctacaaaatc gccatagaca ccgtgagtgt ggttgcacgg gtagtatctc aggaaggtat
     6601 ccatgtccgt atccagcttc acgtaggcat gtggccattc gatctgaacc ttctccgaca
     6661 gcttcttttc ttctgcttcg gagagcttaa cgaactctcc cggtactatt gtcatgatgt
     6721 actcgccgtt ctgtctcgca agcctggcaa gggtaactcg cccaggtgcc gcaaaatgct
     6781 ttacggcagc tcctccggcc gggaagtaga agtcctcggg atacagttct acgtgtttga
     6841 gattgtcttc gggattcata gattttcctg caaagtaggt cgcgtgagtg cccgagttac
     6901 agaggtcgag aaggtcttct tcctcgaagt agtgtctcac gtctgcaaac agaactggag
     6961 actgagcgat atgcttgaag atttccatgg tcagagctcc atccatatcc gtttcggtgg
     7021 ccgcaacgat tgtttccttt gaaccgtgcc agtcatacgg atcattgagg aaggcctcta
     7081 ctatgtccat cgttgcgtag ttgtttgtca tctcgggctg acctttgaat ccaacaaaat
     7141 ccagttcgta ctcttttatg atacttagcg cggcctcgta gagcgcgatc tgcttctcca
     7201 gtttttcagg tgtaagctgt ttgccgtcat actttacagt tgcaagcttt tcaagccact
     7261 ctctagctgc ttttgtgacg tcttttggta ctttctccgc cctgactacc agttcatact
     7321 ggtcgatctg ttccacatcg acaccgaact ccttcagcca ctgatcaccg ttggccgaag
     7381 ccgtgtacat tcccatcgga cggccgccga acataccgta tctttcaccc ttcagtcttg
     7441 cggccgctga agcagccctc acgaagtgca ttaccctttc aattacatct gaatcttcgg
     7501 gattgcccca taccctttca ggcattattc cgacctgttc gagggcacct gctgccgcca
     7561 gctggcctac cataccgggg taagagggat tgatctgtcc ataggtgagg aaaggacctg
     7621 gtgcataaag cgaggccatt acggaaagat gcggccagca ccagatagcg tagttgaata
     7681 tcgtggcctc aacctcggcc ttcatcagtt ccttgccggc cttcttcgcg agggaaggct
     7741 tccagactat atcggaggct gtgacaactt ccacttcacc cgttgcttca agggccttaa
     7801 ccagcctgtc ttgaaacccc ttgttcattt cgatcagatc gttgtgaacg aaatcgcgcc
     7861 cgtcggaaaa agtgatcact ccaacctttc tctttttcat tgagcattcc tcctcttttc
     7921 ggtgttttgt tcgagcttgt ctatatgagc tttgacacag attttctttg aagcagttcc
     7981 gccttcagca caactttcga tttatcacct cttgttccgt ctatttcgct cagcataatc
     8041 gtggcggcaa tcctgccgat gtccttcaca ggctgtctga tcgtcgtcaa gggtggctcg
     8101 atcagttcgt tcatggaatg gtcatcgaag cctacaatcg acagatcacc ggggattttc
     8161 aggcccatcc gccttacggt tgcaactatg acctcattga gaaactggtt tgtggatatg
     8221 atcgcatccg gtctcctgtc cgatttcaga acagactcca gtgacagcga agccctttcc
     8281 gcgtccactg cattctcgat aatccagtct gcgtttattt caatgtccag catcttgcaa
     8341 gcctccaaaa agcccatacg tctctcttct attgtataga ttcccttttt gtgaaggact
     8401 attccgagtt tcctgtgacc gttctccttt aagtgcaaca ctccctggag aatcgcctgt
     8461 cggttgtcga gggttaccga gcttatccta tcaagtcctt cgagaatcct atcgtagaag
     8521 actacgggga tgtttctcct gactgcctct ctgtaaagtc gtgagttgtt tcttccatcc
     8581 tcacccccac agggcgagga aagtatcccc tcgacgccgt gttcgaggag ccatttgatg
     8641 tagattctct ctttctcgaa atcctcgccg atgtcgcaga cgatgaattt gtagcctcta
     8701 ggaaagagca cggtctcgac tccgctcatg aaatccagaa agaatggatt cctgatatcc
     8761 gacacgatga gacccacagt ctgggtcttt cttgtcttca gggattttgc ggaggaattg
     8821 ggaatgtagt tcaatctctc ggccgcgtcg actatctctt tcctcaggga aggactcact
     8881 cccggctcat cggaaagcgc tcgcgatacg gacgacggtg atatgttcaa ttcgtttgct
     8941 atgtctttta tcgttgtctt cttcatcact ggggatcgca acctttcgca aacgtttgcg
     9001 ttcattttca ttatactatg ggtcgtcctt gattgaaaag caaagtggta gcagacagac
     9061 gtagagagag ctcaagtaga ataagtcagt ctttgtgtgt tttccttgat agggagagta
     9121 ttccaagtat gtttggattg ggttctatag aggaaagctc aatctttacc ttctttcctg
     9181 gaactctgta gtagttctct tcaatggtct tgagtattct gtcgaaatag taccggttct
     9241 tcccgagact tcctccgatt accagtaccg aaggatcgag agaatctatg aggttgcaga
     9301 gaaagcgtcc aagagtttca gaagcttcgg ttatcatttc tttgaagacc ggctcgttca
     9361 actctgcgcg atctgttatc tcttttgtgc cgatctcgat tccaagtgaa tcatgtgctc
     9421 taatttcaag gcccctgccg cctgaaattg cttgcaggca accgcgacga ccacaagtgc
     9481 aaagcgggcc atccgctttc actattgtgt ggccgatctc tcccgctgcg ttgcttgctc
     9541 cccgcaagat tttcccccgg acgaccattc ccattcctat tcccgtactg acggtaacgt
     9601 agatgagcga ctcaatatcc ttcctgaatt cccattctcc aaaagccgct gcattacagt
     9661 cgttttcgat tacaccttcg gtaccgagaa aggaggaagc tcttttgaag agctccacgt
     9721 ctttccaccc gaggttcggc gaccagatca acttgccctt ttcgggactg acggcacctg
     9781 ccgtgcaaag cccccagcgg tttatatttc tacttccttt catgcgaaga cagatgttac
     9841 taagtcgctc gaagaaactg tcaattcctt cagctggcct ggtgggaaat tcttccgatg
     9901 aataaggctc cacttctttc tcatcgaaag ccgatacgag agtcttggta cctccaacgt
     9961 cgatcgctat gtcaactgct ggcatttact gctctccgaa tttccaggat cctttcaaga
    10021 acctcccctt ctctttcctc atcaaaaagg tttctgtttg ctatgtagga accgattccg
    10081 agcgcaacgg cgcctgcttt catgtattct gtagcgtttt cggccgttac tccgccgaaa
    10141 ggcatgaatt tgactgtggg aaacggaccc ctgtacgctt ttagcaaatt cagaccctct
    10201 gcatcgccgg ggaagagttt cagaaagctg aatccgttgt tcagcgccgt ctgtatatct
    10261 gtagaagtgt agactccggg tatataactt attccatccc ttgtcgacag ctttgcgatt
    10321 tcctcactaa atccagggct gagaacgaaa tcggcattcg agcgaagcgc tctcgaataa
    10381 gccgtatcat cgtatactgt accggccccg attacgagct ttccgtagaa ttccttcttg
    10441 agttcgtgca gtatctcaga tgaagattcg tcggagaaac tgacttccag aatcgtcaag
    10501 tcgttttcca gcagcatttc acacaacttc ctcgattgat caagcgtaag acccctaatt
    10561 actgtcaaga ccttttctgt cattagtctt tcaaacacgg taaaataccc ctcctttcgc
    10621 ttaccggcag aagataatct ctgcatttag tatatcagga ggtatcgcca catttccccg
    10681 ctgaaaagga aactgtccat ttgagtatta tgtcttttgt ggcatatcgt tgctcatttt
    10741 gatgtaataa tgtgcttctg aaaacatttt cagatctctc atcgctattc aacagagaca
    10801 cccgcgtaaa gtgtcgaaac atttccatgg ttttcaaccg tgctatagaa ttcgaactct
    10861 attagagaga ctcctatttt tagagctttg aaatcgaata accgctttcc gggttcggtg
    10921 cttgctattg gaaagttcga gatgtcaccg ggaaaggatt ggctcttcga agggtcgatg
    10981 tacctgttat ctatgagtct caggatttcg ctatcgcccc gcacgctcca tgaaaatcca
    11041 gccgataagt cttcctcagt agtggctgcg aatagatcac ccgacttatg aaacccggag
    11101 aactgtcaag gaccgttaca taatcaggaa tacactcact taaagttctg tcatccattt
    11161 ctacattgaa tacccttatg tcgacagctt tttcttcgcc ttcccattct ctgtagagct
    11221 tgaaaataat tgcaaagctt cccaaggttc tggactgaat aaccaggtca ctttcgaacc
    11281 gccaccttgg acagcttttt cagattggtc aacggagctt tccttcgaaa aaagtgaaaa
    11341 ggcgttagct tctgatgaat agtagtgcca tgtataaccg gtcgaagctt tctccacaag
    11401 ttgggccttc aagcgttgtt cgtagctgat tctgtttaat tatcgggata tttgattctt
    11461 ctgaatctcc tatgccggca agaagagttc catagaaagt cacaaataca atcgtcatta
    11521 ttaaagagat tatgtgatag tctttgaaag ccataagtac cacctattct gcttctgtta
    11581 gaaaacctaa caatgtaggt atttgacgat ctccagagag aaaggatcaa acgggaagaa
    11641 aggatccaaa gaagaagtcc tgttttcagc taaactcttc tcttgtattt catagaggag
    11701 gaatgtcaaa acgaccgaat tcaatcgcgt ctaatcgtga aagacaagct ccaatgatgt
    11761 ctttcgactt ttcatctttt atctaatagt gtagattttt atgactcaac aaatcaatca
    11821 tttgtgttaa tatttcttca tgctatgaaa aaggggggaa agtaaatgga atccgtttac
    11881 aaaaagcttc gtatatacaa cttgataatg ggttttctcc accttgttca gggaatcata
    11941 atgctgatag taagcaacga ttcttctttg acgattacga gaaactacct ggagttcaat
    12001 caggagataa tgcggctggt tccggtgagt agcccagggg gatttcaccc ccaggctctc
    12061 tcagaaccgg acttgaat
//
LOCUS       DAOQYI010000044        11922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_5850_length_11922_cov_78.658886, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000044 DAOQYI010000000
VERSION     DAOQYI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5850_length_11922_cov_78.658886"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1001
                     /locus_tag="PKL93_09100"
     CDS             99..1001
                     /locus_tag="PKL93_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ71453.1"
                     /translation="MNRSKEKSSGTKSVILLILTGLLWSTGGLLIKNVDSNPLAISGI
                     RSMIASLVILVALGRPKFTWSLSQIASALSYSATVILFVSANKMTTAANAILLQSTAP
                     IYVALLSVWILKERAKLLDWVTIIMVFGGMALFFFESLDSRGMTGNILAVASGVTFAL
                     FNIFMRLQKDGSPLESVLLGNILTAAIGVPFIVGSLPSPSGWISLFILGVAQLGLSYV
                     FYSEAIKHSTALEAILILMIEPILSPVWVFLFLGEIPGKLPMIGGAVVIGAITIRFVL
                     RNLTGRPIFLKYGVPRRSRRRNST"
     gene            1088..2473
                     /locus_tag="PKL93_09105"
     CDS             1088..2473
                     /locus_tag="PKL93_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNQ71454.1"
                     /translation="MHSDEAYSAVINKMLSGGADFAEIFLEERYTGSINMVQGEVEGS
                     VSGKLFGAGLRAFKGTRSIYAYTNELSLDGLMKVAERLRAVISQDRVADSVIDFRNEE
                     FVDVCPVLFSPNNVPKKEKVRVMQLAHEGASTFASTISQVVVNFVEYDQKVWIYNSEG
                     VKAHDRRVRTRLAISAVATKDGQMESGFYGPGAAMGFEFFNMVDPRAAGERAARIADR
                     MVRAEFAPAGRMPVIISNEFGGVIFHEACGHALEATGVAKGASVFAGKLGQKIANECV
                     SAVDDPTIPNAWGSANVDDEGTPTRRNLLIDKGVLKSYMIDRLGGIKMKMQPTGSARR
                     QDYTFAPTSRMSNTFLLPGEYYPEEIIAATDYGLYAKSLGGGSVMPSTGEFNFAVKEG
                     YMIEKGRITRPVRGATLIGKGNEVLTKIDMVGNDLERGQGMCGSISGSIPADVGQPTV
                     RVSELIVGGRN"
     gene            2473..>2909
                     /locus_tag="PKL93_09110"
     CDS             2473..>2909
                     /locus_tag="PKL93_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNQ71455.1"
                     /translation="MDFNTFADKAFKISKEKGFNEAEIYYSKGGEFQLSSLNGEIDSY
                     TDASSIEVYFRGLKNGKIGTAFSEILSEEAATLLVEEAFENCSIASGEDVYIFHDGTG
                     EYRDFEGYSGAFQKKTVKEKIDTVVSLEKLALEYDKRIVMVPT"
     assembly_gap    2910..3009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3010..3692
                     /locus_tag="PKL93_09115"
     CDS             <3010..3692
                     /locus_tag="PKL93_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNQ71456.1"
                     /translation="NVKSGKYRVVFRNDVFGQLFGTFTSMYSADNVQRGLSVLKGKMG
                     TVIGSSKLTVYDDPFLSESPYSKPFDDEGVPTRKKALVEKGELKTFLYDLRTAAKDGT
                     KSTGNSVRGTYRSKPSIAPVNTVVRSGDLSFDALLESMGEGILVTDLTGLHSGTNQVS
                     GEFSLGASGFFVKDGKISNPIEQFTISSNILKVYSGILEVGSDSVSSIYRVSSPSVLI
                     DEIDVASE"
     gene            complement(4075..5400)
                     /gene="glnA"
                     /locus_tag="PKL93_09120"
     CDS             complement(4075..5400)
                     /gene="glnA"
                     /locus_tag="PKL93_09120"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNQ71457.1"
                     /translation="MTEKELIQKCESGEIGFVRLQITDINGMLKNVEVPARMLPTILE
                     KGAMFDGSSIDGFVRIEESDMYLRPDLSTFAFLPWDNCQGKTIRLICDVYKPDGTPFE
                     GDPRYVLKRVIQRAKSMGFETYAGPEPEFFIVKRDHDRGKPIGAFLDTGSYFDLLPVD
                     GGEEVRKQIVLSLQEMGFEVEAAHHEVAPSQHEIDFRYTDILKTADNIQTFKWVVKTI
                     AIADGFHATFMPKPFSGVNGSGMHIHMSLFSDGNNAFYDSSKDHGLSQTALSFVGGII
                     SHAKEITAVTNPTINSYKRLTPGYEAPVNISWALGNRSAMIRIPSTRGEGTRLEFRSP
                     DPTCNPYLALALTLAAGLDGVEKRIDPPNPVDENIFKMKGSRRKELMIENLPGTLINA
                     LEFTRNSEFVRSTLGDHIFNTFVRSKEGEWSEFCASVTDWEISKYLELY"
     gene            5536..6960
                     /locus_tag="PKL93_09125"
     CDS             5536..6960
                     /locus_tag="PKL93_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HNQ71458.1"
                     /translation="MNIKRAFVSVHDKSRVEKIAGYLVSNGVEIVATDHTAEYLMDHG
                     VEAIKVADYTGFPSIFDGRLKAIHPKIIGGILAIQDLPAHIEEMREHSILPFDMIVMN
                     LKPFESAVKESPDESNLSKEIDISGPALLMAGAKNHRDIVVISDPMDYDEVIDSLEDC
                     GDVPLLKRRRYALKAIYAALLYNSSIHKALSQIFASEKYDYTVMEHVMQLLYGDNPSQ
                     KAHLLKLTKEPGLLDEVQIGNPAIGLKKSQLRNINVFMELYYYSDNISLFLDKGKITK
                     VFAGKVPDDFSMGGPDTMFASTFAIDGDTLKRLSDNGVEAFGGVFDRDAIRAARERDI
                     MILTVPAHEAVTLSKETYSGFGNYFVRELTFEMKKYDLDTDGRLALYCSRVNRANNFS
                     IFKDGESILLKSGLSYEELQSYDFGSHDLRDSVMASDNDLTDDLIVRILASGVRQIIL
                     PGTKRTYSNRNILVLGIDFTRLNN"
     gene            7006..7267
                     /gene="ffs"
                     /locus_tag="PKL93_09130"
     ncRNA           7006..7267
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKL93_09130"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            7349..7816
                     /locus_tag="PKL93_09135"
     CDS             7349..7816
                     /locus_tag="PKL93_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNQ71459.1"
                     /translation="MLLKGEKAIDFSLFGSDLKEYKLNEYLGKKVVLVFYPGAFTSVC
                     QKELCTFRDSLANFESLNATILGISMDSPFANKAFKEQNSLSFQLLSDIGGEVSGKYG
                     GVHQDFAGVRGLTVSKRAVFIVNKDGKISYTWVSDDPRVEPDYAEISNHLKKI"
     gene            7861..8364
                     /locus_tag="PKL93_09140"
     CDS             7861..8364
                     /locus_tag="PKL93_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNQ71460.1"
                     /translation="MTEFSFPEHSTELLETLRDGRVLIGVTDGVETNLITVGWGFLGY
                     TWNRPVFIAMIRPSRFTHDFFRSSDGFSVNFMSAKWQEALDFCGTRSGKYFDKFKETG
                     LTAREGLSPSIVVVDEADKVLECRVVSKEALTPLALNGSIASTFYGDNSYHSLIFGEI
                     TDSYTLP"
     gene            complement(8347..8820)
                     /locus_tag="PKL93_09145"
     CDS             complement(8347..8820)
                     /locus_tag="PKL93_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71461.1"
                     /translation="MIHLKKRDRGAISVAYARRKEKAYKYFTAAGVIVGLLSIVLIVT
                     ANQMSTNIVQDADSIRPIFIAGIVFAPISFFIYVFALITSLSAGIRNWNLVLGFLSSF
                     QAVISLIFARDILLLDSSLKLSLQTPNWSSYILWSILVASLLLTFFVGVFSRKSV"
     gene            9828..10751
                     /locus_tag="PKL93_09150"
     CDS             9828..10751
                     /locus_tag="PKL93_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ71462.1"
                     /translation="MDILKKKWMAALTAVFCSILWGSAFPVLKLSYEEMSIAPDDVSA
                     KLVFAGIRFLGAGIMVLMLSIPLGRKGRTRFAKKDYLHLLALGTLQTFLLYFFFYNGL
                     SYTTGMKSSIIMAGENFLVILLAHYMYKNDRLSWKKTLGMILGFVGVFLANFEVGFTL
                     SFVFLGDGFMLIAATIGAFGTMYAKWMSARRSPFLVSGWQLSIGGFLLLIAGFPGMKE
                     DSLVFTLKGNLLLVYSMFLSAIAFSLWYAILSFHKAGEITMFRFVIPVAGVFLSAAFI
                     PGEVLNPYMAFALVLVSAGIVVVNWRNRGVS"
     gene            complement(11050..>11922)
                     /locus_tag="PKL93_09155"
     CDS             complement(11050..>11922)
                     /locus_tag="PKL93_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ71463.1"
                     /translation="MVVAHFLLNLSLIQGGMLGFIIAAVSPAVVVPEMLRLKERDFGK
                     RNEIPSTILAAASIDDVVAITLFTVFLGIEGGQDVSIGMSFLMVPVEIVLGISLGAII
                     GFLFSLLFKRFHIRDTKKVLLILSVAFVFHKLEEVLPAATLLGVMAIGFVLRERLPVA
                     ADRIAGKMERIWVVAEIFLFVLVGASVNISAIGDSWLMGLLVILLGLIFRSAGVLAAT
                     LGSKLDIRERLFCMISYLPKATVQAAVGGIPLAMGVAYGDVILSVSVLSILTTAPLGA
                     IGIRLAARRFAKTS"
BASE COUNT         3155 a         2569 c         2871 g         3227 t
ORIGIN      
        1 cccaacctcc aaccccgcga aagtctaata cttgtctact ttgagcttct gatgtattct
       61 tcttccatgg ggattgaagc agaatcggca ggtgaattat gaaccggagt aaagagaagt
      121 cttctggaac aaagtctgtg atcttattga ttcttaccgg tcttctttgg agcaccggag
      181 gtctcttgat caagaacgtc gactcaaatc ctcttgcgat atcggggatc aggagcatga
      241 ttgcatccct ggtaatactc gttgcccttg gaagaccgaa attcacatgg tccctgtcac
      301 agatagcctc tgccctctct tattcggcca cggtgattct ctttgtttcg gcaaacaaga
      361 tgacaacggc tgcaaatgct attctgcttc aatcgactgc gcctatatat gttgctctgt
      421 tgagtgtatg gatattgaag gagagggcga agctcctcga ctgggttacc attattatgg
      481 tctttggcgg tatggccctc ttcttctttg aaagcctgga cagcagggga atgacgggaa
      541 atattctggc ggttgccagt ggagttacct tcgctctttt caacatcttc atgaggcttc
      601 agaaggatgg ttcacctttg gagtcagtgc ttctagggaa catactgacg gctgcgatcg
      661 gagtgccttt cattgtggga tctctaccga gtccttccgg atggatcagc ctctttattt
      721 tgggcgtggc acaactggga ttatcgtatg ttttctattc tgaggcaatc aaacattcca
      781 cagccctaga ggccattttg attctcatga ttgagcctat tctcagtccc gtttgggtct
      841 ttttgttctt gggcgagatt cccggaaagc ttcccatgat tggaggagcc gtagtcatag
      901 gtgccattac gattaggttt gttcttagaa atctcaccgg cagaccgatt ttccttaaat
      961 atggagttcc caggagatcc agaaggagaa attccaccta accttagatt tcaattcctt
     1021 tgttaagacc tcaatctcga aaatgttgta gaattgtaag aatacctaac aagattggag
     1081 gtgcatcatg cattcagatg aagcatactc cgcagttatc aacaagatgc tgtctggcgg
     1141 agcggatttt gcagagatct ttttggaaga gagatatacg ggaagtataa acatggttca
     1201 aggtgaagta gaagggagcg tgtcggggaa gttattcgga gctgggttac gtgcctttaa
     1261 aggtacaaga agcatctatg cttacacaaa cgaactttcg ctcgatggtc tcatgaaagt
     1321 tgcagaaagg ctgagggccg ttataagtca ggatagggtc gcggattcag ttattgattt
     1381 tagaaatgaa gagtttgttg acgtctgtcc agttctcttt tcaccgaata atgtaccgaa
     1441 aaaagagaag gtaagagtta tgcaacttgc ccatgaagga gcatcgacct tcgcttcgac
     1501 catatctcag gttgtcgtaa atttcgttga atacgatcaa aaagtctgga tctacaattc
     1561 cgaaggcgtg aaggctcatg atcgccgcgt aagaacaaga ctggcgatat ccgcagttgc
     1621 cacaaaagac ggacaaatgg aaagtggttt ctacggaccg ggagcagcaa tgggctttga
     1681 gttctttaat atggtcgatc ccagagccgc cggagaaagg gctgcaagaa tcgcagacag
     1741 aatggtaagg gcggagtttg ctcctgctgg acgaatgcct gtgataatct ccaacgagtt
     1801 tggaggagta atattccacg aggcctgcgg ccatgcgctc gaagctacgg gagtagccaa
     1861 aggcgcatct gtatttgccg gtaagctagg tcagaagata gcaaatgaat gtgtgtctgc
     1921 tgtagacgat ccgacgattc ccaatgcctg gggatcggca aatgttgacg atgaaggtac
     1981 tccgacaagg aggaatctat tgatcgataa aggagttcta aaaagctaca tgatcgatcg
     2041 ccttggtgga ataaagatga aaatgcaacc tactggaagc gcaagaagac aggattacac
     2101 gtttgccccg acatcaagaa tgagcaatac gttccttctt cccggagagt actatcccga
     2161 ggagataata gctgccacag actatggtct ctacgcgaag tctctcggtg ggggatcggt
     2221 tatgccttcc acgggtgaat tcaactttgc ggtaaaggaa ggatacatga tagagaaggg
     2281 aaggataacc agaccggtaa ggggtgcgac acttatcgga aagggcaatg aggtccttac
     2341 gaagattgat atggttggca acgatctcga acgggggcag ggtatgtgcg ggtcgatctc
     2401 gggttcgatc ccggccgatg tgggccagcc aaccgtgaga gtgtctgaac tgattgttgg
     2461 ggggcgaaat tgatggattt caataccttt gccgacaagg ctttcaaaat ctccaaagaa
     2521 aagggcttta atgaagccga gatctattac tcgaaaggtg gagaatttca gttaagctct
     2581 ctgaatggag aaatcgattc atacacggat gccagttcga tagaagttta cttcagaggc
     2641 ctcaagaacg gaaagatcgg cacggcattt tcagagattc taagcgaaga ggcggcgaca
     2701 ttgctggtcg aagaggcttt tgagaactgc tcaatagcga gcggagagga tgtctatatc
     2761 ttccatgatg gaactggtga gtaccgtgac tttgaaggtt attcaggagc cttccagaaa
     2821 aagaccgtga aggaaaagat cgataccgtg gtatctctgg agaagctggc tctcgaatac
     2881 gacaaaagga tcgtgatggt tccgacatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnng aaatgtgaag agtggaaagt acagagtcgt tttcaggaac gacgttttcg
     3061 ggcagctttt cggaactttc acctctatgt actcggcgga caacgttcaa agaggccttt
     3121 ccgtcttgaa aggaaaaatg gggaccgtaa ttggatcaag caagcttacg gtgtacgatg
     3181 atcccttcct ttccgagagt ccttacagta agccatttga cgacgaagga gtgccgacgc
     3241 gtaaaaaagc gcttgttgag aaaggcgagt tgaagacttt cctgtatgac ttgagaacgg
     3301 ccgcgaaaga cggtacgaag tccacgggga actccgtaag gggtacttac agatcaaagc
     3361 cttcaatagc cccagtgaac actgttgtta gatcgggaga cttgagtttc gatgcactcc
     3421 tcgaaagtat gggcgaaggc attctggtca ctgatcttac cggtcttcat tccggcacga
     3481 atcaggtttc gggcgaattt tcgcttggag cgagcggctt tttcgtgaag gacgggaaga
     3541 taagtaatcc catagagcaa tttacgatat cttcgaacat attgaaggtc tatagtggca
     3601 ttttagaagt tggcagcgac tcggtttcat ctatctatcg tgtgagttct ccttcggtct
     3661 taatagatga aatcgatgtg gcttctgaat aataacggag gtgactcttt tgcccttata
     3721 cagttttgtc tgcaaagaat gtggtcacga ggagagtctt cttttgggga tgagcgctac
     3781 ggtgcctccc tgtgaaaaat gcggggggat tctctcaaag cagataacta atatttcgag
     3841 cttcggatca tcctcgagtt ccgatagctg cggctcatgc tccggcggaa gctgttcaac
     3901 ctgcggacac tgattctttc ggacatttaa gtgcccggat aatctgcccg ggcacttctg
     3961 tttcagtaaa tccgaatgaa ttcacttgac ttcaggataa tttgactgaa aatctaaatt
     4021 catcgagaaa agttagactg cttttatcct tccaacctct tggtgagact ttcctcaata
     4081 cagctccaga tacttactga tctcccagtc ggtgaccgaa gcgcagaatt cgctccattc
     4141 gccctctttt gatctgacaa aggtgttgaa tatatgatcg ccaagtgtcg acctgacgaa
     4201 ttcgctgttt ctggtaaact caagagcatt gatcaaagtc cctggaaggt tttctatcat
     4261 cagttccttt cttctcgagc ctttcatctt gaaaatattc tcatcgactg gattaggcgg
     4321 gtcgatcctc ttctcaacac cgtccagacc ggcggcaaga gtcaatgcta acgcaagata
     4381 aggattgcat gtgggatcgg gacttctgaa ttccagcctt gttccttctc cccttgtgct
     4441 gggtattctg atcattgcgc tcctgtttcc cagcgcccaa gagatattca cgggggcctc
     4501 gtatccaggt gtaagtctct tataactgtt tatagttgga tttgtgacgg cggtaatctc
     4561 ttttgcgtgt gagatgattc ccccgacgaa actcagagcg gtttggctta acccgtggtc
     4621 ttttgaggaa tcgtaaaagg cattgttgcc gtccgagaag aggctcatat ggatatgcat
     4681 tcccgatccg ttgacgcccg aaaagggctt cggcataaac gtggcatgaa atccgtcggc
     4741 tatagctatc gtctttacaa cccatttgaa tgtctgaata ttatctgcgg ttttcagtat
     4801 gtcagtgtat ctgaagtcaa tttcgtgttg tgaaggggcc acctcatgat gtgccgcctc
     4861 aacttcaaac cccatttcct gaagagaaag cacgatctgt ttccttacct cctcacctcc
     4921 atcaactgga aggaggtcga aataacttcc cgtatccaga aatgctccga tcggttttcc
     4981 acggtcatgg tctctcttga ctataaagaa ttctggttca ggaccggcgt aagtctcaaa
     5041 ccccatactc ttggctcgct ggataactct cttcaaaaca tatcttggat ctccctcaaa
     5101 tggtgtaccg tcaggtttgt agacatcaca gatgagtctt atagtttttc cctgacagtt
     5161 gtcccaggga aggaaggcga aagtcgaaag atccggccgt aggtacatgt cggactcctc
     5221 gattcgcaca aagccgtcga ttgaggatcc gtcgaacatt gcaccctttt ccagaattgt
     5281 aggcagcatt cttgcaggaa cttccacatt cttcagcata ccgtttatat cggttatctg
     5341 aagcctgacg aaaccaattt ctccagattc gcatttctga atcaattctt tttcagtcat
     5401 gttactcccc cgctatcctt gcccgaccca agggcatgag atactaaagt atacgccaaa
     5461 ctgataacat tataaattat tagttgaatt tttcagggat tgattgatga taaactaatc
     5521 acatggaggt gcccgatgaa cataaagcgt gcctttgtca gcgtacatga caagtcaaga
     5581 gtggaaaaga tcgctggata tctagtctcg aacggtgtag aaatcgtcgc cactgatcat
     5641 accgcggaat accttatgga tcatggtgtc gaggcaataa aggtcgctga ttacactggg
     5701 tttccttcga tattcgacgg aaggctgaaa gctatccacc caaagattat aggtggaatt
     5761 cttgcgatcc aggatcttcc tgcccatatt gaggaaatga gagaacattc gattttgcct
     5821 ttcgacatga tagttatgaa tctcaagcct tttgagtccg ctgtaaagga atcaccggat
     5881 gagtctaatc tctcaaaaga gatcgatatc agtggaccgg ccctgctgat ggcgggcgcc
     5941 aagaaccaca gggacattgt agtcatatcc gatcccatgg attatgatga agtgattgac
     6001 agtttagagg actgtggaga tgttcccctt cttaaaagaa gaagatacgc tcttaaggcg
     6061 atctacgctg ccttgcttta caattcttcc attcataagg cgttatcgca gatttttgct
     6121 tccgagaaat acgactatac ggtcatggaa cacgttatgc agctgcttta cggggacaac
     6181 ccctctcaga aggctcattt gctaaagctt acgaaggaac ctggtctgct tgatgaggtt
     6241 cagatcggta atcccgccat tggactcaaa aagtctcagc tgagaaacat taatgttttc
     6301 atggagcttt attactacag cgacaacatt tcgcttttcc ttgataaagg taagatcaca
     6361 aaagtctttg cgggcaaggt accggacgat ttttccatgg gcggtcccga tacgatgttt
     6421 gcttcgacct ttgccatcga tggtgatact ttgaagagac tgagtgacaa tggcgtagaa
     6481 gcttttggag gcgttttcga ccgcgatgcc atacgcgctg caagagagag agatataatg
     6541 atactgaccg taccggccca tgaagcggtg acactatcga aggaaactta ttcgggcttc
     6601 gggaactatt ttgtaagaga actcacattc gagatgaaga agtatgacct tgatacagac
     6661 ggaaggctcg ctctctattg ttcacgcgtg aacagggcga acaatttctc aattttcaaa
     6721 gacggtgaga gtattcttct caagagcggt ttgagttacg aagaacttca gagttacgat
     6781 ttcggcagtc atgatttgag ggattctgta atggcttctg acaatgattt gaccgacgat
     6841 ctgatagtaa gaatacttgc ctccggggtt agacagataa tccttcccgg tacgaaaaga
     6901 acctattcga acagaaacat tctagtgctt ggaatagact tcaccagact gaacaactag
     6961 agatcacttg aaagctgtga aagcagaata tataatgtat atacaccatg ctaggtgggg
     7021 agctggcggt tccctgtacc cgaaatccgc catacgcggg gccgaatgcc ttttgaggct
     7081 ttgccggttt ggtgtcggtt tgtctaaagg tgttgaggat tgagtcctgc gcaacgggaa
     7141 cttgcgaacc tggtcaggtc cgggaggaag cagccataag cgagcgctct cgtgtgccgc
     7201 ggggttgctc agtctgagcc ggaaggcaaa tacggccagg tcgggaatgt cgatggagga
     7261 tgcatggtgt ttcttcttct atgtatcgca cctaatcatc tgttagaatt aatatgacaa
     7321 taatagacgg ttttaggagg tacatgggat gttattaaaa ggagaaaagg cgatagactt
     7381 ctcactattc ggttcggatc ttaaggaata caaactcaac gaatatctag gaaagaaagt
     7441 tgtactcgtt ttctaccccg gtgcgttcac gagtgtatgt caaaaggaac tatgtacatt
     7501 cagagattct cttgcgaact ttgagagcct taacgctaca attctcggga taagtatgga
     7561 cagccctttt gccaacaaag ctttcaagga gcaaaattcc ctttcctttc agcttctctc
     7621 agatattgga ggagaagtat ccgggaagta cggcggcgtg catcaggact ttgccggcgt
     7681 aaggggtctc actgtttcaa agagagccgt atttattgtg aataaagacg gaaagatttc
     7741 ctacacatgg gtttcagacg atcccagagt tgagccagat tatgcagaaa tctcgaatca
     7801 tcttaagaag atttagcgat ggggggataa cccccaccgt ttttgttaga ggaggttagt
     7861 atgacagaat tcagctttcc ggagcactcg acggagcttc ttgaaaccct cagggacggg
     7921 agagttctga tcggagtaac agatggcgtt gaaacgaacc tgataaccgt tggctggggt
     7981 ttcctgggtt acacatggaa tcgacctgtc ttcattgcca tgataaggcc cagcagattc
     8041 acgcacgatt ttttcagaag cagcgacgga ttctcggtca atttcatgtc agcaaagtgg
     8101 caggaggctc tggatttctg tggaacaaga agcggaaagt atttcgacaa gttcaaggaa
     8161 accggattga ctgctagaga ggggctgtca ccttcaatag tcgtagtaga tgaggctgac
     8221 aaagttttgg agtgtcgcgt tgtttcaaag gaagcgttga cgcctttggc tctgaatggt
     8281 agtatagcca gcacattcta tggcgataat tcttatcatt cgctcatctt cggcgaaatt
     8341 acagattcat acactcttcc ttgagaatac tcctacaaag aatgtgagca agagagaggc
     8401 tactagaatt gaccagagaa tgtatgacga ccagttggga gtctggaggc tcaacttcag
     8461 agaagagtcc agcagaagaa tatctcttgc gaaaatcagg gagattacgg cttgaaagga
     8521 agacaggaat cctagaacga ggttccaatt ccttattccc gcgctcagtg aagtaatcaa
     8581 cgcaaaaacg tagataaaga atgaaatagg cgcgaacact attcctgcga tgaagatagg
     8641 cctaatcgag tctgcatctt gcacaatgtt cgtcgacatt tggtttgccg tgactatcaa
     8701 aactatcgaa agcaaaccaa ctatgacacc tgcggccgta aagtatttgt aagccttttc
     8761 ctttctgcga gcatatgcaa cgcttattgc cccgcgatct ctcttcttca aatgaatcac
     8821 ccagacagct ttttagaaaa ctaaaacggc ttaaagccgc aaaacacaca ttatttctgc
     8881 attaactcta acgaataaat cttgcagaaa cattatcatt atactgcaat ttatagtgat
     8941 tatcaaaaac taatttagaa ccaataattg tatcgtttat gcctatgcat tcgcttgact
     9001 ttaatgctta ttttcttttc ctcttcgtag agaaaagttc aggactgctt caagtgaaac
     9061 aagtgatctt ctctccgggt attttttgtt agggaaaggc atcttcatgg gtagaaaaac
     9121 cttgagaggc tcagtttgaa gacatcattg aacaggtata aggaaatttc tctttaagat
     9181 aagaaactta aaagagcttt cactgggtga gattcccgtg ctgatatgat caaaccacaa
     9241 aattcagcaa aggccttatt ttttccagtt cttctattcc gatccctcga ttgaaaatct
     9301 tcaaggtcac atttgtctgc cagatcgcca gaaatcatct agctcagatt gttgtgcggt
     9361 tttctggaag ctcgagaagg gataggcatg acgaagcgca tactgattat cacactgctg
     9421 gtaattctct tgaccttatc ttcttgtgta aaaaaggatc agcatctcat aacccttact
     9481 gcaaatcctg cagaggtctg aacaagggac ctctttgctg cggttgcgag acagcctttg
     9541 ggattgattg catagttatg cgtgttggca aactatcacc agttcttact tgcctggtgc
     9601 agaatacgat gtaagacaga gatcaaatga cacttcagca aagctttccc taacttcatg
     9661 catgtgctgc agtatatcat cgaagccctt attgctgcgt aaactgggat cttgattttc
     9721 atatgacggg tgtcactaaa gaattttcgg gaccatctca atgattgttc atgtttagcc
     9781 ccgctcattt tgcagttcac cttacatttt tagtgtagac taatttcatg gatattttga
     9841 aaaagaagtg gatggcggcc ttgaccgcgg tattctgctc tattctctgg ggcagtgctt
     9901 ttcccgtgct gaaactaagc tacgaggaaa tgtctatagc tccggacgac gtttcagcca
     9961 aacttgtctt tgcgggaatt cggtttctgg gggccggtat catggtgctc atgctttcaa
    10021 taccccttgg tagaaagggt agaacaagat tcgcgaagaa ggactacctc catttgctgg
    10081 cgctcggaac actacagact tttctacttt acttcttctt ctataacggg ctgagctaca
    10141 cgacgggtat gaagtcttca ataataatgg caggagagaa ctttctcgtt attcttcttg
    10201 ctcattacat gtacaagaat gaccgtttga gctggaaaaa gacgcttggt atgatactgg
    10261 gattcgtcgg tgttttcctt gcaaattttg aagtcggctt cactctgagc ttcgtatttc
    10321 tgggagacgg attcatgttg atcgcggcga ctatcggtgc attcggcacc atgtatgcca
    10381 aatggatgtc agctaggcga tctccctttc tcgtgtcggg atggcagctt tcgatcggcg
    10441 gctttcttct cctgattgca ggctttcccg ggatgaaaga ggattcgctt gttttcacac
    10501 tgaagggaaa tctccttctg gtatattcta tgtttctttc ggccatagcc ttttccttat
    10561 ggtatgcgat tctcagcttt cacaaagccg gtgagatcac tatgtttcgt ttcgtgatcc
    10621 cggtggccgg tgtctttctc tcggcagcct ttattccggg agaggttcta aatccgtaca
    10681 tggcatttgc cctcgtattg gtttcggccg gtatagtggt tgtcaactgg aggaacaggg
    10741 gagtctcata agataattcc gcttgataaa ttcgaatacc gctctggaaa ttcacatcgt
    10801 gttgctgtgg gttctttacg agccgtgacc ccgccctatc accaaataga aatgcaagcg
    10861 atttttttcg agcagaatcg ttccggcaga aagatgcggc gaagaaaaga aaactgaagg
    10921 aagacgactc aattctaatt ttaacgtaac taggtaaagg atagccgacg gcttctgacg
    10981 aatctagaac agtctttaca tttgcatata acgagaaaga tggtcgaaga gaaaactgaa
    11041 tggattctct caactggttt tcgcaaacct tcttgcagcg agtcttatgc caatggcgcc
    11101 aaggggggcc gttgtaagga tcgatagaac cgaaaccgag agtatcacat ctccgtaagc
    11161 tactcccatt gctaagggta ttcctccaac tgcagcctga actgtggctt tgggcagata
    11221 ggaaatcatg cagaagagtc tttcgcggat atccagtttc gaaccgagag tggccgccag
    11281 aactcctgcg cttctaaata tgagcccaag tagaattacg agcagtccca taagccagga
    11341 gtcgcctatc gcacttatgt tgacacttgc gccaaccaga acgaataaga atatttctgc
    11401 gacaacccag attctctcca tcttccccgc aatcctgtcg gcggctaccg gaagtctctc
    11461 tcgaagtacg aaacctatcg ccatcactcc caggagagtt gctgccggga gtacttcctc
    11521 gagcttatgg aagacgaatg caacagatag gatcaagaga accttcttgg tgtccctgat
    11581 atggaatctc ttgaacagca agctgaagag aaacccaatt atcgccccaa gagatattcc
    11641 gagaacaatc tctaccggca ccataagaaa agacattccg atactgacgt cctgtcctcc
    11701 ctcaattccc aaaaacactg taaagagtgt aatggccaca acgtcgtcta ttgaagctgc
    11761 cgcaagaatt gtagaaggta tttcgtttct ctttccgaaa tccctttctt tcagtctcag
    11821 catctcggga actactaccg caggcgacac cgccgcaata atgaagccta gcattccgcc
    11881 ttgaatgagg ctgagattca gcaggaagtg agccactacc at
//
LOCUS       DAOQYI010000045        11846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_5886_length_11846_cov_75.470274, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000045 DAOQYI010000000
VERSION     DAOQYI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5886_length_11846_cov_75.470274"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1397)
                     /gene="mggS"
                     /locus_tag="PKL93_09160"
     CDS             complement(93..1397)
                     /gene="mggS"
                     /locus_tag="PKL93_09160"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglucosylglycerate synthase"
                     /protein_id="HNQ71464.1"
                     /translation="MRVGLIHYRAGLMDGVSLEMEKWRKVFSRMGHSVEIIAGNNAPG
                     VDIVIPEIEYNDEKNEILNSKLYGSTPSSESKLLDELSIRTAEILGSFRSVLSDFDLL
                     IPNNIWSLGWSIPAGLALHDFAEESGKPFISHNHDFWWDRPYYSSPYRSVMELLENYF
                     PPDLPNVKNLTINSISASDLFERRGIRATVVPNVMDFSGLVSISDERIRTLKSEAGIE
                     KGDIVFLQATRITERKAVEISIRLIAAFNNIAGSLKGKKLYNGEPFTGRAVLVYSGLT
                     ERQSMDYRKKLDELANKLGVKAIDISSSSSLSQAAFFESYGVADIVIYPTVFEGWGNQ
                     LLEALVYRKPLALFRYSVFKSDIQNSGISFVDLGDRYIYSDGLAEIPEQTILSAVKEI
                     TELLFDREKYEETAEKNFQIGREKFSLETLGGIVKGLITDLV"
     gene            1592..2263
                     /gene="elbB"
                     /locus_tag="PKL93_09165"
     CDS             1592..2263
                     /gene="elbB"
                     /locus_tag="PKL93_09165"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNQ71465.1"
                     /translation="MKCGVVLTGCGSEDGTAVDEAIMTFLALDKAGVEVVSLALDKDQ
                     YEVINHQTGKPAELEQKRNQLVESARLLSGPVNNIDTFNWEDLDLLVIPGGKGVLKSL
                     STFEDEEDHYRVNKSLERLIVNCFFGKKPLGAIGEGVLILARALENVASNLTVTASGN
                     PEDRMTVVEKLAIDHVPCEVGEMCVDKTNKVVTAPLLVRENGLMEKNESIETFVKRLF
                     DLLPG"
     gene            complement(2285..3232)
                     /locus_tag="PKL93_09170"
     CDS             complement(2285..3232)
                     /locus_tag="PKL93_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNQ71466.1"
                     /translation="MIDIRKVFLSLRRLLPFLGIALFTVSIYVLVRELSRYTISDIAA
                     KVGEIPASRLVIAAFLTFLTYLNLSFYDLIASRRTGVKLKFASTALVSFIGYTFSKNI
                     GFTFLSGTSVRFRLYGKWGVAGGKILAVITLNYLTFWLGFFALSGILFTLWPPVLQNA
                     VTVPFGSLKAIGIASAGIYVLYVIVVSFRKRPFRIKGRSFTLPGKSFTLVQTGISILD
                     WLLSAGVFFMLLPPEIGYLEVLGVFLLSQFAGLSSQVPGGLGVFEALTVVILSPSLKP
                     DILVAALIIYRIIFYLVPFIIAVVSFAVNELLYRGSEKA"
     gene            complement(3232..4107)
                     /locus_tag="PKL93_09175"
     CDS             complement(3232..4107)
                     /locus_tag="PKL93_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HNQ71467.1"
                     /translation="MLHRIGRDRVVYDMSPEHKPVLEVSPGDTVIVETEDCFCHRIVL
                     ESQTVGGDFDFTHVNPATGPIAVKGAMPGDTIVVSIESIELDSQGVVETCPFWGPVSN
                     VSRECVTEIVKIDGNFMSFLGERIEIKPMIGVIGNSPAEGSVSCTTPGSHGGNLDTKD
                     IAAGSRVYLPVFVKGGNLSLGDVHARMGDGEVGGTGVEIRALVRLNVDIDKMPVESPT
                     VETEEAFYLLFSAKTLEEASRGAVKRAIEFISDWKSIPAERAYMLTSITCDLMISQVV
                     NPLVTVRVRVPKEIL"
     gene            complement(4175..5068)
                     /locus_tag="PKL93_09180"
     CDS             complement(4175..5068)
                     /locus_tag="PKL93_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HNQ71468.1"
                     /translation="MLYLLLAILSSSSIAMIFKVTEGRSYNRLAVTTFNYLSAFLVAL
                     VMIAVERPAVGPGGGSPAEVIVKGERLFSLTSSVVWGLSLGLVSGLFFFLSFIFYQKS
                     VWESGASLSGAFGKLGILIPMILSLLVWKEYPEALQWVGIGLAIFSIVLVNNPFGSGR
                     QNFKAALIMLFITGGLAEFMNKLFQNYTVSGYKNVFLFSVFASAFSISAFFLKRSGKS
                     FRKKEAIIGLIVGLPNLFSSFFLIMALEEMKGSVVFPVYSAGSVVMISLGSLMFFGEK
                     LKKLEMISLGMVLISLIIINI"
     gene            5173..6192
                     /locus_tag="PKL93_09185"
     CDS             5173..6192
                     /locus_tag="PKL93_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HNQ71469.1"
                     /translation="MAERLIEVFSGSDPEKDLKEMLEEKSIIDYWTVKTSEELWQTKI
                     LALAENSEKILDSLEQRFTQDESFRAILFQVEASLPRAQEEEKEEEKQATEEEKKPEK
                     EIFKRISREELYHDISEMISNDAVDIAMIVLSAIVATIGLVRNSPAIIIGAMVIAPML
                     GPNVAQSFATTLGDFGLLAKSVRTNLIRIGLGFAFALVFGLLFRLDYTTNEITSRTVA
                     NLGDIVLAFASGTAAALSISSGVSTSLIGVMVAVSLMPPLAAAGLLLGSGNIDGFSGA
                     ILLFFVNIVCINLASIITFRLQGIEPRSWWEANKAGKAVRVTIIGWVVVLGLLALSLF
                     LSEVL"
     gene            6263..6586
                     /locus_tag="PKL93_09190"
     CDS             6263..6586
                     /locus_tag="PKL93_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ71470.1"
                     /translation="MKLVKSSIEKGKVALDRPGLKGRIIYDRENAQAVVIDIAEGMEL
                     AEHKTPVDVFILVLAGRGIITIGGDKHEVMKDDIIDSPKMIPHGIVNTGEETLRVLVV
                     KAPHP"
     gene            complement(6669..7700)
                     /locus_tag="PKL93_09195"
     CDS             complement(6669..7700)
                     /locus_tag="PKL93_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71471.1"
                     /translation="MENKKAGNIHVRFASIDDNEKLLKIERESAQEGSIWLVAFREDF
                     FGRLKYFEEGFIMIAENAYDIIGCIGVGIDNLIVNGEIKKGIYLFGLRTNPQYRLKVA
                     RWLKSIIQELQNLLGPTDFDFGYASVKADNTASKKILEHMGFSTTATLDFYACPVRKT
                     SQEKSVFVEREVDLETILDLYKPLEKDHDLLLQGTKAFEVMVTERRLRLFKTEGAYAL
                     VLDTSGEQDFGITRLSKGLRAFQLLAQGTISPLVRIPKMNERLRSWDVLLFGWEDIRS
                     ARKIVRKIHRCAWEEGITLINFSRDRSLGSMKGAVGPLSFRIPFEIMIYEKNSINRGS
                     RPIIRTPTI"
     gene            complement(7708..9657)
                     /locus_tag="PKL93_09200"
     CDS             complement(7708..9657)
                     /locus_tag="PKL93_09200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNQ71472.1"
                     /translation="MKKLKLEDLYKYSAVGDLHIFPGGENFVFVRKTMNKKENQYESN
                     IWLGNMKNEEVRQLTRGGKDGSPVVSPDGKSILFVSKRDKEAKGTGLYLLPLEGGESR
                     LVKTLKGGFSSVSWIDSETVLFMTKHAPGEDPEKIEEDEPPEKKVYEIDRIPFVSNGA
                     GFTENRVGHLYKMKLENGKMEPIEAVGGDIESIVLSPERKSVAVITTEDREKRPIWSS
                     LYLLDLQSETAKRIGDDSLSFYHCEWSDDSELFAVATDFKKGFPTNPFIVHVDLESFT
                     LTTLAREMDLYFGNSLNSDVRGVSPNTSMKVVENKLYSLVTAGSQIKLISMDKEGKVE
                     EIAVSSGSIDCFDIAAEALLLSEMTTTEPLEIYSLVKGKRKKLTDFNSWMKGIKLSKP
                     ESFEVEASDGQKIEGWIMRPFNFEEGKKYPAVVEIHGGPKTAYGGGYIHEFQTLASEG
                     YAVIYCNPRGSAGYGTDFADIRGHYGERDFEDIMEIVEYVINECDFVDEERLGVTGGS
                     YGGFMTNWIVGHTDAFKAAVSQRSISSWISFFGTTDIGYFFASDQTGGDFFDNLEGYL
                     RQSPLMSAPNVVTPILFIHSLEDYRCWVPEAMQFFTALRYLGKEAKMVLFPKENHELS
                     RGGLPIHREKRLKAILEWFDSHLKI"
     gene            9756..10358
                     /locus_tag="PKL93_09205"
     CDS             9756..10358
                     /locus_tag="PKL93_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HNQ71473.1"
                     /translation="MILVTHFDPFGGSKINASQIVVELLADIREDIELMSLPTVFDDC
                     FSRLELRLLDNVPEALIMVGQAAGRSLVTPEKIAINWKESPTPDNNGFIATGEKIIPA
                     SPDAYFSRLPVDGIVSKLKDEGLPIEKSFSAGAFVCNYLFYRCMDFLTKRAINIPAGF
                     VHIPCIPEQIGPGEERPAIEAEVSARVLSRVIDLAVEENR"
     gene            10355..11104
                     /locus_tag="PKL93_09210"
     CDS             10355..11104
                     /locus_tag="PKL93_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNQ71474.1"
                     /translation="MRIGGVPLNIVWESPEINMKIIERVFAGASEAGVELIVFPETTL
                     SGFTNNAERAYVKDACGFFRKQVDHWGIPAVYGFIDRRDSKFYNCAAFLAPGSEPLIY
                     RKRKLFSYGGENIVYSPGDTNLSFEMKNIHFSLNICYDLRFPELFRANLPAEVMIVIA
                     NWPEKRANHWQALLKARAIENQSFVLGLNRTGVDGNGIEYDEAFSVLFDQDGNEVPFE
                     AEKGLLLWELDYERMSILRDWRERFPALKDI"
BASE COUNT         3022 a         2999 c         2857 g         2968 t
ORIGIN      
        1 agatcggcct gggcgaaagg agctacaaac tcgagaaagt agcctgcttc ggcagataga
       61 aacttggatt cgtgttagcc tgattactta gtctaaacca gatcggttat caatcccttt
      121 actatgccgc ccagcgtctc caggctgaac ttttctcttc ctatctggaa gtttttttcc
      181 gcggtttctt cgtatttctc tctgtcgaag aggagttccg tgatctcttt aacggcgcta
      241 aggatagtct gctccggtat ttctgccaat ccgtcgctgt agatatacct atcgcccaga
      301 tctacgaaag agatcccgct gttctgtata tcgctcttga atacgctgta tctgaaaagg
      361 gcaaggggct tcctgtacac aagcgcctcc agaagctggt tcccccagcc ctcgaatacg
      421 gttggatata taacgatgtc tgcgacgccg tacgattcga agaaggccgc ctggctcaag
      481 gaggatgaag aggagatgtc gatcgctttt actccgagct tgttcgcaag ttcatctagc
      541 ttttttctgt agtccatcga ctgcctttcg gtcaatccgg aatacacgag gacggccctc
      601 ccggtaaacg gctccccgtt gtaaagtttt ttccccttca aagatcccgc gatattattg
      661 aaagccgcga ttagccttat agagatctcg accgcctttc tctccgttat cctggttgcc
      721 tggagaaaga cgatatcccc cttctctatc ccggcctcgc ttttgagcgt tcgaatgcgc
      781 tcatcggaga tagagacgag cccgctgaag tccatcacat tcggcaccac ggtagccctg
      841 atacccctcc tctcgaagag atcggaagcg gagatcgagt tgatcgtcag gttcttgacg
      901 tttgggagat cgggagggaa gtagttttcc aggagctcca tgacgcttcg atagggactg
      961 ctgtaatagg ggcggtccca ccagaagtca tggttgtgag agataaacgg cttacccgac
     1021 tcttccgcga agtcatgcag ggccagtccc gcaggtatag accatccaag cgaccagatg
     1081 ttgttcggta taagaagatc gaagtcagag agaaccgatc tgaagctgcc tagaatctcg
     1141 gcggttctga tggagagttc atcgagcagt ttgctctccg atgaaggcgt actgccgtag
     1201 agcttcgaat tcaggatttc atttttctcg tcgttgtatt ctatctccgg aatcacaatg
     1261 tcgacaccgg gagcgttgtt tcccgcaatg atctccaccg agtgacccat tctcgagaaa
     1321 accttgcgcc acttttccat ttcaagggaa acaccgtcca tcagtccggc cctgtaatga
     1381 atcaaaccca ctctcataag accaccccct tgccgaatag tcacatgaac tcactactaa
     1441 tatccaataa tctcgccggc atgtcaacag tccagatggc caacacactt acgagccgtt
     1501 tggaacgggg ggcaaagatg ataaaatcca ttaatattta tattttccta aggaaaaacg
     1561 actcttgatg cttctataag gagggaccga attgaagtgt ggagtagtgc ttacgggttg
     1621 tggctctgag gatggaacgg ccgttgacga ggccataatg accttcctcg ccctggataa
     1681 ggcaggggtt gaagttgttt ctctggcact ggacaaggat caatacgagg ttattaacca
     1741 ccagacagga aaaccggctg aacttgaaca aaagcgaaac cagctcgtag aatcggcaag
     1801 actgctcagc ggaccggtaa acaacattga tactttcaac tgggaggatc tggacttgct
     1861 cgtcattccc ggcggtaagg gagtcttgaa gagcctatct acttttgaag acgaagagga
     1921 tcactacaga gttaataaga gccttgaaag gcttatcgtg aactgcttct tcggtaagaa
     1981 acctctggga gcgatcggag aaggtgtttt aatacttgca agggctctcg agaacgtagc
     2041 ctccaatctt accgtcaccg cttcgggcaa tcccgaagat aggatgaccg tcgtcgaaaa
     2101 gctggccatc gatcacgtcc cctgtgaagt cggagagatg tgcgtcgata agacaaacaa
     2161 agttgtaact gccccgcttc ttgttcgaga aaatggactc atggaaaaga atgagagcat
     2221 cgagactttc gtcaagagac tgttcgacct gctgccaggt taaacccacc ataacggttc
     2281 tccctcaggc cttttcagaa cctctataaa ggagttcgtt gactgcaaaa gaaaccactg
     2341 cgataatgaa gggaacgagg tagaagatta ttctatagat tattagcgct gccacaagaa
     2401 tatccggctt taaggagggc gagagaataa ctactgtaag cgcctcaaag actcccagac
     2461 ctccggggac ctgactcgac agacctgcaa actgcgacag caggaagacg ccaagaacct
     2521 caagataacc gatctccggg ggaaggagca tgaaaaacac tcctgcggaa agcagccaat
     2581 cgagaatgga tatgccggtc tggaccagcg tgaaagactt tccaggaaga gtgaagctcc
     2641 ttccctttat tctgaacggc cgttttctga agctaaccac tatcacgtag agaacataga
     2701 tcccggcaga ggcaattcca atagccttca atgatccaaa gggtaccgtt acggcattct
     2761 gaagaacggg aggccataga gtgaacagga tacccgaaag ggcgaagaaa ccaagccaga
     2821 aagtcagata gttgagggtt attactgcga gaatcttccc tcctgcaaca ccccactttc
     2881 cgtataacct gaagcggacg cttgtacccg aaagaaacgt gaacccgata ttcttgctga
     2941 aagtgtatcc aatgaaagaa acaagagcgg tcgacgcaaa tttcagtttg actcccgtcc
     3001 ttctggaagc gatcagatca tagaaactga gattgagata ggtcagaaag gtcaggaagg
     3061 ccgctatcac aagcctggaa gccggaatct ccccgacctt tgcagcgata tcgctgatag
     3121 tgtatctact caactcacga acgagcacgt agatcgagac agtgaaaaga gctattccca
     3181 gaaagggcag aagccttctt aaagagagaa atacctttct gatgtctatc actagagaat
     3241 ctctttcgga actctaactc tcacagttac cagtggattc accacctggc ttatcattag
     3301 atcgcacgtg atgcttgtca gcatataggc tctttcagcg ggaatagact tccagtccga
     3361 gataaattcg atggctctct tcaccgcccc gcggctcgcc tcttccagag tcttcgcgct
     3421 gaacaagagg tagaaagcct cctcggtttc gacggtggga ctttcaacgg gcatcttatc
     3481 tatatctaca tttagcctca ccagggccct tatctcgact cctgtgccgc cgacttcacc
     3541 atcacccatc ctcgcatgaa cgtcgccgag ggagagattc ccccccttca cgaagaccgg
     3601 aagataaact ctcgaaccgg ccgcaatatc ttttgtgtcg aggtttccgc catgacttcc
     3661 cggagttgta caggaaacgg aaccttcggc cggagagttt ccgattacgc ctatcatagg
     3721 ttttatttcg atgcgctctc ccagaaacga catgaaattg ccgtcaatct tgacgatttc
     3781 ggtaacgcac tccctggaga cattgctaac cgggccccag aagggacagg tctcgacaac
     3841 gccctgactg tcaagctcta tgctctctat actgactact atcgtgtcac ccggcatcgc
     3901 tcccttaacg gcgatgggcc cggtagccgg attcacatga gtgaagtcga agtctcctcc
     3961 gacggtctgt gactctaaga cgatccggtg gcagaagcaa tcctccgtct ccactattac
     4021 agtatccccc gggctgactt ccagaacggg cttgtgttca ggcgacatat cgtacactac
     4081 tctgtctctt ccaattctgt gtaacacggg acgcacctcc actgactgat attgtctcaa
     4141 tactacctcg acgttgtttt aatccaaaga gatatcatat gtttattata ataagggaaa
     4201 tcagcaccat gcctaaggat atcatctcga gcttcttgag cttttcaccg aagaacataa
     4261 ggcttccaag acttatcatc acaacgcttc cggcactgta aacgggaaag accacggaac
     4321 ccttcatttc ttcgagggcc attatgagaa agaaggatga gaagaggttt ggcagtccca
     4381 cgatcagtcc tatgattgct tccttcttac ggaaagactt accggacctc ttcagaaaga
     4441 acgcgcttat cgaaaatgcg cttgcaaaga cgctgaagag aaagacattc ttgtatccgc
     4501 tgacggtgta gttctggaac agcttgttca taaattcggc cagtccgccg gtaatgaaca
     4561 acatgattaa agcggccttg aaattctgcc tccccgatcc gaaggggttg tttacgagta
     4621 cgatcgagaa gatggcaagg ccgatcccga cccactgcag cgcttccgga tactccttcc
     4681 agactagcaa agaaagaatc atgggtatta gaatccccag ctttccgaag gctcccgaaa
     4741 ggcttgcccc gctttcccag acactcttct gataaaagat gaaggagaga aagaagaaga
     4801 ggccggaaac gagtcccaaa gacaaacccc agactacgct gctggtgagg ctgaaaagac
     4861 gctctccctt cactatgact tcagccggag atcccccgcc gggacctacg gcaggtcttt
     4921 caaccgctat catgacgagc gctaccaaga acgcgctcag gtaattgaac gtcgtaaccg
     4981 cgagcctgtt gtagcttctt ccctccgtca ctttgaaaat cattgctatg gaggaactgg
     5041 aaagtatcgc cagcaaaaga tataacactc caacctccct gatccgatgc cttctcttct
     5101 tatttgcagg tgtaatatat tcttggcgag caagacaatt atgccacagt ctctctacgg
     5161 gatggtgatt agatggccga aagactgata gaagtattct caggttccga ccccgaaaaa
     5221 gatctgaagg aaatgctgga agagaaatcg ataatcgact actggacggt taagacatcg
     5281 gaagagcttt ggcagacgaa gatactggcg cttgccgaga acagcgagaa aatcctcgac
     5341 tctctcgagc agcgcttcac ccaggatgaa tccttcaggg caatactctt tcaggtagaa
     5401 gcctcgcttc caagagctca agaagaggaa aaagaagaag agaaacaggc tacggaagag
     5461 gagaagaagc ccgagaagga aatcttcaag cggataagca gagaagagct ttatcacgac
     5521 atctcggaga tgatctcgaa cgacgcggta gatatagcga tgatagtatt gtcggccatc
     5581 gtagctacga taggccttgt gagaaacagc cccgcaataa tcatcggggc gatggtgatc
     5641 gctccgatgc tagggccaaa cgtcgcgcag tcctttgcaa cgacgctcgg agacttcgga
     5701 cttctcgcaa agtcagtgag aacgaatctg atccgtatcg ggctgggctt tgcctttgct
     5761 cttgttttcg ggctcttatt cagactagat tacactacta acgagataac cagtcgaaca
     5821 gtggccaacc tgggcgacat tgtgctggcg ttcgcatcgg gaacggctgc tgccctttcg
     5881 atcagctctg gggtatccac ttcgctcatt ggggttatgg tagccgtttc gcttatgccg
     5941 ccgctggctg cggcagggct gctcctcgga agcggaaata tcgacggatt ctccggagcg
     6001 atactcctgt tcttcgtcaa cattgtctgt ataaacctcg cttcaatcat aacgttcaga
     6061 cttcagggga tagaaccgcg ctcctggtgg gaagcgaata aggcgggcaa ggctgttagg
     6121 gttacgataa ttggctgggt agtagtcctc gggcttctgg ctctctcgct attcctgagc
     6181 gaagtcctat gatacggtaa catatgttac cgatcggggc tcttcggttt gctaagatag
     6241 tattaacatt agaggcgagg tgatgaagtt ggtgaagagc tcaattgaaa agggcaaagt
     6301 tgcacttgac agacccggac taaagggccg gataatctac gacagagaga atgcgcaggc
     6361 ggtcgtcatt gacatagccg aggggatgga gttggccgag cacaagacac ctgtcgatgt
     6421 tttcatactg gtgctcgccg gtagaggaat aatcactatc ggcggagata agcatgaagt
     6481 aatgaaagat gacatcatag acagtccgaa aatgataccg cacggaatag tcaacaccgg
     6541 agaagagact ttgcgcgttc tggtagtgaa ggcacctcat ccatagatat taaggccggg
     6601 tctaagcatt tccgacccgg cctctcattt actaagtctt cagttcatcc gaaaaaagaa
     6661 ttttcaagtt atatcgtcgg agttcttatt ataggtctgc ttcctctatt tatgctgttt
     6721 ttctcataga tcattatctc aaagggaatc ctgaaactga gaggacctac cgcacccttc
     6781 atactcccga ggctcctgtc cctcgaaaag tttatcagcg ttatgccctc ttcccaggcg
     6841 catctatgga tctttcttac gatctttcgc gcgcttcgga tatcttccca gccgaagaga
     6901 agaacgtccc aggacctcaa tcgctcgttc attttcggga tcctcaccag cggtgaaata
     6961 gtcccctgag cgagcagttg aaaggccctc aacccctttg aaaggcgtgt gattccaaag
     7021 tcctgctcgc cgctcgtgtc gagaaccagg gcgtatgcac cttcagtctt gaataacctc
     7081 aatctcctct ctgtaaccat cacttcgaag gccttagtac cttgaagaag aaggtcatga
     7141 tccttctcga gaggtttgta aaggtccaaa atcgtttcca ggtcgacctc cctttccaca
     7201 aaaaccgact tctcctgaga ggtcttccta accgggcagg cgtagaagtc cagagtcgcc
     7261 gtagtggaga aacccatgtg ctcgagaatc ttcttcgagg cggtgttgtc ggccttaacc
     7321 gaggcatatc cgaaatcgaa atccgtgggg ccaagaagat tctgcagttc ctgaatgatc
     7381 gacttcagcc agcgggcgac tttcagcctg tattgcgggt tcgtccgaag cccaaagaga
     7441 tatatgccct ttttgatctc tccgttgacg atcaggttgt cgattccaac accgatacat
     7501 ccgatgatgt cataggcgtt ttcggcaatc ataatgaaac cttcttcgaa gtacttcagt
     7561 cttccaaaga agtcctctct gaatgcaact agccagatgc tcccctcctg agccgactct
     7621 cgctcgatct tgaggagctt ttcgttgtca tcgattgagg cgaatcttac gtggatgttt
     7681 cctgccttct tgttttccat caacggctca tatcttcaga tgggaatcga accattccag
     7741 tattgccttc aaacgctttt ctctgtgtat gggaaggccg cctctggaaa gctcgtggtt
     7801 ctccttcggg aaaagcacca ttttggcctc tttgcctaga tatcttaacg cagtaaaaaa
     7861 ctgcatcgct tcggggaccc agcatctgta atcttcaagg gagtgaatga aaagaatagg
     7921 agtgacgacg ttgggcgccg acattagagg tgactgcctt agataaccct cgaggttgtc
     7981 gaagaaatcg cctccggtct ggtcgcttgc aaagaaatat cctatatccg tagtgccgaa
     8041 gaaggaaatc cagctcgaga tagacctctg ggagacggcc gccttgaagg cgtcagtatg
     8101 tcctacaatc cagtttgtca tgaagccgcc gtatgagccc ccggtgacac cgagcctctc
     8161 ctcgtcgacg aaatcgcact catttatcac gtactcgacg atttccatta tgtcttcgaa
     8221 atccctttcc ccgtaatgcc cccttatatc ggcgaagtcg gttccgtaac cggcgcttcc
     8281 cctcgggtta cagtagataa ctgcataccc ttcggaggcc agagtctgga attcatgtat
     8341 atatccgccg ccgtatgccg tctttggccc tccgtggatc tccactacgg ccgggtattt
     8401 cttcccttcc tcgaagttga aaggcctcat tatccagcct tcgatctttt gaccgtcaga
     8461 ggcctccact tcaaagctct ccggcttcga cagctttata cccttcatcc acgagttgaa
     8521 gtctgtgagc ttcttcctct tgcctttgac aagagagtag atctccagtg gctctgtcgt
     8581 tgtcatctcg gaaaggagaa gcgcctcggc cgcgatatcg aagcagtcta tactccccga
     8641 agagacagcg atttcctcga cctttccctc tttatccata gaaatcagct ttatctgcga
     8701 cccggcagtt acgagtgaat acaacttgtt ttctactacc ttcatggacg tgttcggcga
     8761 gacgcccctc acatcgctgt tcaggctgtt gccgaagtac aggtccattt ccctggcaag
     8821 agtagtaaga gtgaaggatt ccagatcaac gtggactatg aagggattgg ttgggaagcc
     8881 ctttttgaag tctgtcgcga cggcgaagag ttcgctatcg tctgaccact cgcaatgata
     8941 gaaagagaga ctgtcgtcac cgattctctt tgcagtttca ctctgcaaat cgaggaggta
     9001 tagactgctc catatcggcc tcttctccct gtcttcggtt gttattaccg cgacgctctt
     9061 tctctcggga gataaaacga tgctttcgat atcgcctccg acggcctcaa tcggttccat
     9121 ctttccattt tcgagtttca tcttatatag atggcccact ctgttctcgg tgaatccagc
     9181 gccgttagag acaaatggta ttctatctat ttcgtaaacc ttcttctctg gcggctcgtc
     9241 ttcttcgatc ttctcgggat cttccccggg ggcatgcttc gtcatgaata gaaccgtctc
     9301 cgaatcgatc cacgagacag aggagaatcc tcctttaaga gtcttcacca gtctgctttc
     9361 accgccctca agaggcagaa gataaagccc ggttccttta gcctccttgt ctctcttcga
     9421 gacaaagaga atgcttttgc cgtctggact tacgacggga gaaccgtcct tcccgccccg
     9481 ggtcagctga cggacttctt cattcttcat gttgcccaac cagatgttgc tttcgtactg
     9541 gttctccttc ttgttcatcg tctttcttac aaagacaaag ttttccccac cgggaaagat
     9601 gtgaagatcg cccacagcgg agtatttgta gagatcctca agcttcagct tcttcaacta
     9661 gatcacccct ttcagaatca ttctaccaga atgacggctt ttcattctcg acttccttat
     9721 gatgctaaaa ttatagggaa tgagaggtgt aaaagatgat actcgtgacc catttcgacc
     9781 ccttcggggg aagtaaaata aacgcttcgc agatagtcgt ggagcttcta gcggatatca
     9841 gggaagacat tgagctcatg agcctcccaa cggtcttcga cgactgcttt tctcgactgg
     9901 agctgcggct cctagacaac gtcccggagg cgttgatcat ggtcggccag gcggccggga
     9961 gatctctcgt tactccagag aagattgcga tcaactggaa ggaatcccca actccagaca
    10021 acaacggatt tattgcgacc ggtgagaaga taattcctgc ctcgcccgac gcatactttt
    10081 cgaggcttcc ggtcgacggg atcgtctcga aacttaagga cgagggtctt cccatcgaga
    10141 aatctttttc agccggcgca tttgtctgta actacctctt ttacaggtgt atggactttc
    10201 tcacgaagag agccataaac atccctgccg ggttcgtaca tataccgtgc attcccgaac
    10261 agattggtcc gggagaagag agacctgcaa tagaggcgga agtctcggct agggtccttt
    10321 cgagggtaat agatcttgca gtggaggaga atagatgaga ataggcggcg ttcctctcaa
    10381 catagtttgg gagtctccag agataaatat gaagataatc gaaagagttt tcgccggggc
    10441 ctcggaagca ggtgtcgagc taattgtttt tcccgaaact accttgagcg ggttcactaa
    10501 taacgccgaa agagcttacg tgaaagatgc gtgtggtttc ttcagaaagc aggtagatca
    10561 ctggggtatt ccggcagttt acggctttat agacagaaga gactcgaagt tctacaactg
    10621 cgcggctttt cttgccccgg gttcggagcc tttgatatat aggaagagaa agctgttttc
    10681 gtacggaggg gagaatatcg tttacagccc cggtgacact aatctctctt tcgagatgaa
    10741 aaacatacac ttctctctga acatctgtta cgatcttcgt tttccggagc tcttcagggc
    10801 caacttgcct gcagaggtca tgatagtgat tgccaactgg ccggaaaagc gagccaatca
    10861 ctggcaggct cttctcaaag cgagggcgat cgagaaccag tcatttgttc tggggctaaa
    10921 caggactggg gtcgacggca acggcataga atacgacgag gccttttcag tactcttcga
    10981 tcaagatggt aacgaagtac cctttgaagc agagaaggga ttgcttctct gggaactgga
    11041 ctatgagaga atgagtattc taagagactg gcgggagaga ttccccgccc taaaggatat
    11101 atgaggctat aacccctagt atagggtcct atctgattcc cgtttcaata cctctctcat
    11161 gaagaattcg tcgacgtttt tcatcgaaat tccggtgaat cttttctcgt ctatgtcgac
    11221 tacaactccg tcgcaacagg gtcctgtaca atgcggagac tttacagtag taagattgcc
    11281 tgaactatgt tcactaacca gatccatgag cctcttactg acctcggaag agcattacat
    11341 aaggcacacg cttccagtgc aaacttcgat aatcaagggc aatcttccgt ttatgactcc
    11401 ttccacaaca aattataagc ttacagaaga tgaagtctaa atctaactca aaggtacata
    11461 ttcttaatct ccttcatctc gactctaagt aaattctatt ctgatgcatt aactctcttg
    11521 agtagactta atcatgatcc acttcacctg tcaggatcac cccgaacttg atccgggatc
    11581 cgggctttga aatacagtga tcggggattg aaggttcgtt gtaggaagag caagaaagac
    11641 cggttgtccg ttgaatggtt ctccgttctc aaagaagaac ggttgcaagt tccaagaggc
    11701 aagcgtaaga ataaatgacg aggttcgggg taggtggttg cgggttggta agagcaaaag
    11761 aaactggttg accgttgagc ggttctccgt tctccgagga gaacccgttg ctcgttcaag
    11821 agcgaggatc tgtctttgtt cttggt
//
LOCUS       DAOQYI010000046        11552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_6043_length_11552_cov_66.850135, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000046 DAOQYI010000000
VERSION     DAOQYI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6043_length_11552_cov_66.850135"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..2210)
                     /locus_tag="PKL93_09215"
     CDS             complement(357..2210)
                     /locus_tag="PKL93_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71475.1"
                     /translation="MRRTFLFLIVLSLAIIFLVSCPALKYALTIQIGGSGTTDPSEGV
                     HEFNKNQVVTVKAFPESGWQFDSWEGEVSTPDSTTTTVTMDKSKTIKAVFVLDQIGII
                     IDKLDPLTPLSFRHGEELILLVPNNIQRFLNLLEDDDPLNRWAAAYALQRNQLDQSTL
                     DDLEDYLDDENLTIRALIAVAFLLSGDESGKSVLVNLLTEDTVMMYSIPPVLLSDFAE
                     VILNAYYPAEYPLTSFSPQTTTVSGGPCEYTVTVKVVFHGAGASETQVEAWETDAEAI
                     WNGADDSREWGDGCCSVKFEFDFSVLAEGAQPPDDAHVVEVKKVKSSHTSWVATPLPT
                     PGSTETTTGEWDSLDTGPVIAHEMGHMMGLDDEYHNDEDGNYVNDNPQPEGSPPSIMA
                     QTWGGAQPLTEHFDAIMEAADIECDCDYTLTIDPPYDVNISPGSHTVTVTVAKEDGKP
                     AKNCDLTLNITGLHPKSDVKLKTDDAGKSQYTYQCVRPDHTGIDNINVTGECGALGDA
                     VKKWIALWDLFPFGITPFDGQEDYPYSIPMAIIFGVEPALALEAENLAFNVSVMMGTE
                     TLFSTKTHHTEIETPLLLEPGSDFTLEIVPISLPDTEGTPTVINFSTLPLE"
     gene            3109..3591
                     /locus_tag="PKL93_09220"
     CDS             3109..3591
                     /locus_tag="PKL93_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ71476.1"
                     /translation="MKYLEFRKMRRSDKELPENEAREMLKKGSFGVLSTADINGMPYG
                     VPINYVYEDGRIFIHCASSGHKLDNISQNSHVSFCVVEYAKVIPERFSTNFRSAIALG
                     KASVVDNVEEKIEALKKLVSKYSSDYIQEGITEINNSLEATSIIRIDVLHLTGKFANG
                     "
     gene            3766..4248
                     /locus_tag="PKL93_09225"
     CDS             3766..4248
                     /locus_tag="PKL93_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71477.1"
                     /translation="MMNRDTVIRITIGTIMVLLGVLTIFVKSFTLNLWLLFIWIPAVF
                     MEYRAFSGRDRNLFVPAGVLITVAVILTLNVIFPGFITEGGWALFIAAPAVGLFQSYF
                     GREEKDKGVLTAASFLSALSILFLLISLAPTVLGIFIGIVLLAVGGLVVYKNLRTIRR
                     "
     gene            4260..5381
                     /locus_tag="PKL93_09230"
     CDS             4260..5381
                     /locus_tag="PKL93_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNQ71478.1"
                     /translation="MKAMILAAGAGTRLRPLTNRLPKPMLPIIEKPVIEFILELLSKY
                     DVKEIMINVSHLAGVLQNYVRSGYRFGVRVGYSFEGHFEKGQLVPEPVGSAGGLKKIQ
                     EESGFFDETFVVLCGDAIVDFDLAEAFEFHKASRSVATIISKEVQIDKVSNYGIIVSD
                     SSGRVESFQEKPPAEKAKSNLANTGIYIFEPEVLEYIPKNQFFDIGGELLPLLVQKKE
                     RVFSLHTKIDWYDIGRNSDYLEILAKALGGEIKGFKPSGKEVTEGLWRGTGSLLEKTT
                     KIITPVYIGPGSVVQKNVKLEGPVMIGANCRIHDDVELSNVFLGDYVRIKPGFKAKNL
                     LITQEYYVGSDGKGGMLADSNLKRYVSDVRTMEASPANK"
     gene            5433..6644
                     /locus_tag="PKL93_09235"
     CDS             5433..6644
                     /locus_tag="PKL93_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71479.1"
                     /translation="MLKIVRVTSRLDRKEFTDLPERLYRDYPLWVPPFSHEMKAILRS
                     RGSQLLANGPHVFFIAKDDNEVKGRIAVGIEQVMNNEKSVKHAYFTLFESVDDREVAE
                     ALLRTAESWARNKGMNYLKGPVSPTNGDDYRGLLVDNFDDPPAVLMPYNPPYYASFFD
                     SFEIYLKYLGFQYDLANVISEREIKFTEIAMDRYKFRVEEANFRRIREVSADLQKIME
                     ESIPNWEEDILPPTYDELYDMAKTLKLVADRRMVIIARSGERPIGFFVALPDFSPIIR
                     EIEGRLLPFGWYKFFRLRKSLERARAAILFVVPDFRNKGVPSAMFLRAYRNIQNMGFK
                     EIEGSSISSMNSTMIANARRAGGKEYKHYIVYGKSLLKRPLSLSEIYGPAASRFVSKG
                     QRNSERVLERI"
     gene            complement(6562..7908)
                     /locus_tag="PKL93_09240"
     CDS             complement(6562..7908)
                     /locus_tag="PKL93_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ71480.1"
                     /translation="MREIIAVVLFFAIASTAMPVAVVFSGGGGRGAYEVGVYGALLDL
                     GQDIEGIYGSSVGALNAAGLISEGFEAARDLWCSMDYSELMNASPQLYELIQGNIAQL
                     ETLELASAMRTLTSNNGIDIEPLRNKMKEIIAEEKVRESEIELGIVLYSLSEMKPYAL
                     YKASIPEGKLIDFVLASANFPAFTREEIDGEVFIDGGVYSNFPLFMAKERGHRDVVAV
                     DLIGMYFGDSLAYVNIFTDGMDVTLIQPSEQFGTVMTFDSEVSKNYLICGYLDTMKAY
                     EVLRGEKYFIFGDEDPLRDHFLSLDSESRIEALKLLGLDGVKDECAEYHYYRQFIPWL
                     ESTLKTPYMKTSELVSSLLEEMADFLEVERLIAYSPSGLLRAVLAAYRADDFRDERDL
                     DYLTIYRRLLVFLEYLHRQKPFEVQSSAEFRSFQELFQSSVGLLKQTYSPRGHISH"
     gene            8073..9803
                     /locus_tag="PKL93_09245"
     CDS             8073..9803
                     /locus_tag="PKL93_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71481.1"
                     /translation="MIRDFLKHNWPRYLYGSIFLILVDLLMLVTPRVIGMIVDEIRGG
                     AQDLNLVGLLIMAVIGVAFGLFITRLFWRVFIMGAARRFEYYTRKTLFEKLLTLPPTF
                     YDKTRVGDLMARFTNDVNAVRMAMGPAVVMIIDAVFLVTVTIFAMGLFTNWSLTWISI
                     APLPALAIVSTFFGKMIHKRFKLVQASFSDLTDTIEESISGVRLIKSYGIEDLRNETL
                     TEKSNLYVDKNMKLVKVWGMFFPLTQTLSMLGSVLATLFGGRMVILGQITLGQYITFT
                     SYLGMLVWPMTAFGWVINIIQRGRASYRRLMEVLEEENNITTNDPVEVGSFEGHVVMK
                     NLTFTYPTGSRPALENIDFEVKPGSKIAIVGTTGSGKSTMAKLIARLYPVEDGMLFID
                     GVDINNLRPETIRDNVAYVPQETFLFSETVGNNIRFGNMEISDEEVERYAKVASIHED
                     IEGFSKGYKTIVGERGVTLSGGQKQRISIARALIKEAPLVILDDCLSAVDTETEAEIL
                     KSLKESETNRSIIVISHRLKAVVDSDEIYVLHDGRIVEKGVHTQLVEARGLYQRMYER
                     QMLEEKLEEE"
     gene            9806..>11552
                     /locus_tag="PKL93_09250"
     CDS             9806..>11552
                     /locus_tag="PKL93_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71482.1"
                     /translation="MAETVYSEVEEKLKVEDWRVVFRLWKYTKPYVPILILALLLIAA
                     STALDLLPPLLMRKAIDDYMDNQYSLVVVEEGDSYNFVQSEEGTFYLHQDESGMYSVT
                     DGTTTYPITEKQLAELRVEDLNGIGQIAIFMILILFSLFFANYGQVYATSYMGQKITH
                     GIRVDLFQHLIRVPMRFFDTNPSGRLATRVANDTQNLAEFFTSVITSMVKDVLLLGGI
                     IVIMLNLSTYLGLITVAILPVIAGAIFVFRYFDRVAYRKVRTRLAIINAFLAEHISGM
                     SITQLFNQEERKKKEFDTVNKSHFKSLMEQLWVFAIFRPLLDLLYYVTIAIVIWFGVK
                     SIIGNTLAFGVLVAFISYIDMFFRPLQDIAEKYDIVQNAMASAEKIFKLMDEEEEKYN
                     SNEPGVKSLEGTVEFENVTFAYDGEHKVLKDISFSVRPKEKIAFVGETGAGKTSIISI
                     LTGLYKIQSGKIKIDGRNIYDYNLQSFRKKVGVVLQDVFLFSGSILDNIRLFDESISR
                     EKVIEAAKYVYAHHFIERLQDKYDTVILERGGTLSAGERQLIALARAVVFNADILVLD
                     EATANVDTETEALIQR"
BASE COUNT         3143 a         2552 c         2828 g         3029 t
ORIGIN      
        1 aaatcattcc ttagaattca agtcatagct tatatatcta caacagacca caggacccgg
       61 tcagtcccta ttttcgctct cgccttctcg tgagcgaatc gaactcacaa atatcctatt
      121 tctcagctct tccaagcgtt cagcgaccag cgaaaagcgg ttcttttatg ggagatcccg
      181 cacagacgca ctgcgagatg acagtccttc acgtcatatg aaaagctcct gttaaggatc
      241 tgggcttggg tctcagcgaa ggacgggtcc atgatctggg acgaaccaag gacggctctt
      301 cacagcgctc agcgtctgct ggcaagcctc ctgtggttct ttgataatga ttcaatttac
      361 tcaagaggca aggtcgaaaa gttaattact gtcggtgttc cttcagtatc cgggagagaa
      421 ataggcacaa tctccaaagt aaaatcgctg ccaggttcta gaagcagagg ggtttcgatc
      481 tctgtgtgat gcgtcttcgt ggaaaagagt gtctctgttc ccatcattac tgagacgtta
      541 aatgctaggt tctctgcttc aagggcgagt gcaggttcaa caccaaagat aattgccatt
      601 ggaattgaat aaggataatc ctcctggccg tcaaagggcg tgatcccaaa tgggaatagg
      661 tcccatagag ctatccactt ctttactgca tctcctaatg ctccacattc ccccgttacg
      721 ttgatgttgt ctattccagt gtggtctggc ctaacgcact gataggtgta ctgtgattta
      781 ccagcgtcat ctgtctttag cttaacatct gatttcggat ggagaccggt aatgttcagg
      841 gtgagatcac agttcttggc tggctttccg tcttctttcg caactgtaac tgtgactgta
      901 tgagaaccgg gagagatatt gacatcatat ggcggatcga tagtaagcgt gtagtcacaa
      961 tcgcattcaa tatctgcggc ctccattatc gcatcaaagt gttcagttag aggctgggct
     1021 ccaccccagg tctgagccat tatactcgga gggctccctt caggctgagg attatcattg
     1081 acgtagttcc catcctcgtc attatgatac tcgtcatcca gacccatcat gtgtcccatt
     1141 tcatgagcga taacgggacc tgtgtcgagg ctgtcccact cacccgtcgt ggtttctgtc
     1201 gatccaggag tcggcagagg agtagctacc caagaggtat ggctactttt cactttttta
     1261 acttctacaa catgggcgtc atccggtggc tgagcccctt ctgcgaggac ggagaaatca
     1321 aactcgaatt tcacactaca gcatccgtct ccccattcac gtgaatcgtc agccccattc
     1381 caaattgctt cggcatcagt ctcccatgct tcaacctgtg tttccgaggc tcctgcacca
     1441 tgaaagacca ctttcacagt gacagtgtat tcgcagggtc caccagagac agtggttgtt
     1501 tgtggtgaaa agctcgtcaa cggatattcg gcagggtagt aagcgttgag gataacttcg
     1561 gcaaagtctg aaagcaaaac aggcgggata ctgtacatca taacggtatc ttcagtcaaa
     1621 agattcacta aaacagactt tcccgattca tctccgctca gcagaaatgc tacagctatg
     1681 agagccctga tagttagatt ttcgtcatcg agataatcct ccagatcatc aagagttgac
     1741 tggtcgagct gatttctttg aagagcatag gctgcagccc atcgattcaa aggatcgtcg
     1801 tcttcaagaa gattcaggaa cctctggata ttgttcggaa ccagcagaat aagctcttca
     1861 ccatgtctaa aggataatgg ggtaagcggg tctagcttat caatgatgat gccaatctga
     1921 tccaaaacga agacggcttt gatagtcttg ctcttgtcca tagtcacggt cgttgtagtt
     1981 gaatcaggcg tagagacttc accttcccac gagtcgaatt gccaaccgct ttcgggaaat
     2041 gcttttactg taactacttg gttcttatta aattcatgga ctccttcgga tggatccgta
     2101 gttccactgc ctcctatctg aattgtgaga gcgtatttta gggcgggaca actaacaaga
     2161 aagatgattg ctaaagaaag cacaatcaag aaaagaaatg ttcgacgcat tgtagcaccc
     2221 ccttcaaatt agtgagcgct tgataggaat caatcgaggt tggaaaatgt aggacacagc
     2281 aaatgactgc ttgacaaagt gattatacat tagtaaaggt gtacgtcaaa gaattcgaac
     2341 ctcacctctt tacaccagtc gaagaactgg gtatcgagca tggagtctcg ggtctggtga
     2401 aaagaaacgg tttgctctac aagaagtgtt atggagcagt atgcaaaggc tatcatcctg
     2461 tataggcttt tggaaccctc tcacactgtc atcccgagct tgtttcggga tctttgtttt
     2521 gctcctctcg gaggacggtg gaccgttgac ggacaacgtt gtcttcacag cgttcagcgg
     2581 gttttcgttt tttagcgtat agcggttctt agtgcgagat cccgtgcaga gtcatcacgg
     2641 gatgacaatt tcagacgatt atgcagttct cttgtagggg cgaacggccg ttcgcccgaa
     2701 ggaggtcttg gttggaatct ctgcctttta cgcgaaaaag ggaccatagg agccggtcag
     2761 tccctatttt cgctcgcagc gttcagcggg tcttcgtact taagcgcaga gcggttctta
     2821 gaacgggatg ctgaaacaag ttcagatgac tgaaaacggc gttttcataa tgacctcaca
     2881 ctgtcatccc gagcttgttt cgggatcttg ttttgatcct cttggaggat ggaggaccgt
     2941 tgacggacaa cgttgtcttc agcagcgttc agcgggtatt acggtcttga gcaagtagcg
     3001 acttgtactt gggttatacg aatgattgag aagaaccttg ctgtgtcttc aaagcaaaat
     3061 gagcggatca tttcgaacat aaggtaaaat acagggtata ggctgggggt gaagtatttg
     3121 gaatttagaa agatgaggcg atcagataag gagcttcctg aaaacgaggc tcgagaaatg
     3181 ctcaaaaagg gttcgtttgg agttctttcg actgccgata taaatggaat gccttacggg
     3241 gtgccgataa attatgtgta tgaagacggt aggattttca ttcactgtgc ttcttccggg
     3301 cacaagctcg ataacatttc tcaaaacagc catgtttcct tttgtgtggt cgaatacgca
     3361 aaagtgattc ctgagcgttt ctctacgaac ttccgcagcg caattgctct aggaaaggct
     3421 tcagttgtcg ataatgttga agagaagatc gaagccctga agaagctggt ttcaaagtac
     3481 tcgtccgact atatccaaga aggaatcacc gaaatcaata actctctgga agccacttcc
     3541 attatcagaa tagatgttct tcatttgact ggaaagttcg caaatggata gtttttctgt
     3601 taaggattag caagatagct tctggatgaa attatgttcg gaaaatcgaa aactgctagt
     3661 tggggatttg atcagattgc gcagtttgaa gtatttcgat tcttctgact aaagaagcca
     3721 tcttcttgtt ataattccga tgaatgaact ctctggaggc gatggatgat gaacagggac
     3781 acagttatta gaattacaat tgggacaata atggttcttc tcggtgttct cacaatattc
     3841 gtaaaatcct tcactttgaa tctctggctt ttattcatat ggattcccgc agtattcatg
     3901 gagtacagag ccttctctgg aagggacagg aatctcttcg tcccggcggg agtactgatt
     3961 acggtagcgg taattctaac tctaaatgtc atcttcccgg gtttcattac cgaaggcggt
     4021 tgggcactgt tcatcgccgc acctgcggtg ggtctttttc aatcttactt cggaagagaa
     4081 gagaaagaca aaggtgttct gacggcagct tctttcttgt ctgctttgtc aatactgttc
     4141 ctccttattt cgcttgcacc cacagtgctg ggaattttca ttgggatagt gttgcttgca
     4201 gtcggaggtt tggtagttta taagaatctg agaacaatca gaagatagga ggaacataca
     4261 tgaaggccat gattttagca gccggggccg ggacgagatt gagaccgttg acaaacaggc
     4321 taccgaaacc gatgctcccg atcattgaga aaccggttat cgaattcata ttggagctcc
     4381 tttcgaagta tgacgtaaag gagatcatga taaatgtctc gcaccttgcg ggcgttcttc
     4441 agaattacgt gaggagtgga tatagattcg gggtgagggt cggctattcc ttcgaaggtc
     4501 actttgaaaa gggacagctc gtccctgagc cggtaggatc tgcaggtggg ctgaaaaaga
     4561 tccaggaaga gtcgggtttt ttcgatgaga cgttcgtggt actctgcggt gacgcgatag
     4621 tcgacttcga tctggccgaa gccttcgagt ttcataaggc cagcagatca gttgccacaa
     4681 ttatctctaa agaggttcag atagacaagg tttctaacta cgggataatc gtatctgact
     4741 cttctggccg agtcgagtcc tttcaggaga aaccacccgc agagaaagca aaatccaacc
     4801 ttgcaaatac ggggatatat atctttgagc ctgaggtttt agagtacatt ccaaagaatc
     4861 agttcttcga cataggggga gagttgctgc cacttctcgt ccagaaaaag gaaagggttt
     4921 tctctctcca tacaaagata gactggtacg atatcggccg aaattctgat tatctcgaaa
     4981 tacttgcgaa ggcgcttggg ggagaaatca agggattcaa accttctgga aaagaagtga
     5041 ccgagggcct gtggagaggt acaggatcgc tcctcgaaaa aacgacgaag ataataactc
     5101 cggtttatat tgggccggga tctgtagtac aaaagaacgt gaaactggaa ggtccggtaa
     5161 tgatcggcgc gaattgcagg attcatgacg atgttgaact aagcaatgtc ttccttgggg
     5221 attacgtcag gataaagccg ggtttcaagg cgaaaaatct tcttattaca caggagtatt
     5281 atgttggctc cgatggaaag ggcggaatgc ttgccgattc caatctgaag cgctatgtct
     5341 ctgacgttag aactatggag gccagtcccg ctaacaagta gagaggtttc tcatgctaca
     5401 attgtccata cgtttttttg aggagtagcc acatgttgaa gattgtcaga gtcactagcc
     5461 ggctggatag gaaagaattc actgatctgc cagagagatt gtacagagat tatccgctct
     5521 gggtcccgcc ttttagccat gaaatgaagg ccatactgag aagcaggggt tcacagcttc
     5581 tcgcaaatgg ccctcatgtt tttttcatcg cgaaggatga taacgaagtt aagggaagaa
     5641 tcgccgttgg catagaacaa gtaatgaaca acgaaaagtc agtaaaacat gcttacttca
     5701 cattattcga atcggtagac gacagggagg ttgccgaggc gcttctgagg actgccgaaa
     5761 gttgggcgag gaacaaagga atgaactatc tgaagggacc cgtttctcca acgaacggag
     5821 atgactacag aggactcctg gttgacaact tcgatgaccc tcctgccgta ttgatgccat
     5881 acaatcctcc ttattacgca agcttcttcg acagtttcga gatctatctg aagtatcttg
     5941 gtttccaata tgacctcgca aacgttatct cagagcgaga gataaagttc acagagatcg
     6001 ccatggatcg ctacaagttt agagtggaag aggcaaattt cagaagaata agggaagttt
     6061 cggccgatct tcagaagatt atggaggaat cgattccaaa ctgggaggaa gacatactgc
     6121 cccctacata cgacgagcta tatgacatgg caaagacgct taagctggtg gccgatcgaa
     6181 gaatggtgat tattgcaaga tcaggagaga gaccgatcgg cttcttcgtt gcgcttccag
     6241 atttcagccc gattattaga gagatagaag gaagactttt gccatttggc tggtacaaat
     6301 tcttcaggct acgtaagtcg ctggaacgag cgagggcagc aattctcttc gtagttcccg
     6361 atttcagaaa caagggagta ccctcggcga tgtttttgag ggcttataga aacatacaga
     6421 acatgggttt caaggagata gaaggttctt caatcagttc aatgaattcc accatgattg
     6481 ccaatgcgag gcgcgccggc ggaaaggaat acaagcatta catcgtttac ggaaagagtc
     6541 tgttgaagag gcccctttct ctcagtgaga tatatggccc cgcggcgagt aggtttgttt
     6601 caaaaggcca acggaactct gaaagagttc ttgaaaggat ctgaactcag cggaactctg
     6661 cacttcaaaa ggcttctgcc gatgaagata ctcaaggaaa acgagaagcc tcctgtaaat
     6721 agtgaggtag tccagatctc gctcatctcg aaagtcatcg gctctgtatg ctgccaggac
     6781 cgccctcagc agacccgaag gcgaataggc gatcagcctt tcgacttcga ggaagtcagc
     6841 catctcttcg agtaatgatg agaccagctc acttgttttc atgtatggcg ttttcaacgt
     6901 actctccagc cagggaatga actgtcggta gtaatggtac tcggcgcact cgtcttttac
     6961 tccgtcaagt cccagtagtt tcagggcttc gattcgagac tcactgtcaa gggacaggaa
     7021 atggtcccgc aagggatctt catccccgaa tatgaagtat ttttcgcccc gtaagacttc
     7081 ataagccttc atcgtatcaa gataaccaca aataagatag ttcttcgaga cttcggaatc
     7141 gaaggtcatg actgttccaa actgttcgga aggttgaatg agcgtcacat ccattccgtc
     7201 ggtaaagata ttcacgtaag caagagagtc tccgaagtac atgccaataa ggtcaacggc
     7261 tacgacatcc ctgtgacccc tttctttcgc catgaagagc ggaaaattcg agtaaacacc
     7321 gccgtctatg aagacctctc catctatctc ttctcttgtg aaggcaggga agtttgcgct
     7381 tgccagaaca aagtcaatta gctttccttc tggtatggag gctttgtaaa gagcataggg
     7441 cttcatttca ctcagtgagt aaaggacaat tccaagttca atctcgctct ctcttacctt
     7501 ttcttcggca ataatctcct tcatcttgtt tctcaacggt tctatatcaa tgccattgtt
     7561 cgaagtaaga gtcctcattg cagatgccag ttcaagagtc tcaagctgcg cgatattgcc
     7621 ttgaatcagt tcatatagtt gcggtgaggc gttcatgagc tccgaataat ccatgctgca
     7681 ccacaagtcc ctggccgcct caaatccttc gcttattaat ccggcggcat tcaaggcccc
     7741 gacagaggac ccatagatcc cctcgatatc ctgtccaaga tccagcagag ctccataaac
     7801 cccgacctcg tacgcccctc tacctccgcc acctgaaaag acgactgcaa cgggcatggc
     7861 agtacttgca atggcaaaaa atagcacaac ggcaataatc tctctcataa tccttctccg
     7921 gtgttggttt agattcggaa aacctgaatt gatgatatta ttatacattg gagaggaaag
     7981 actcttgaaa tgcttttcgg aaattcatcg cactttcccc tgaaatgtag tatgatatta
     8041 agttagttta gcggactaga gaggcgatag ttttgataag agacttcttg aaacacaact
     8101 ggccaaggta cctctatggt tcaatctttc ttattcttgt ggatctgctc atgttggtta
     8161 ctccaagagt tataggaatg attgtggatg agatcagggg cggcgcccaa gatcttaacc
     8221 tagtcggact gctgataatg gcagtcatag gtgttgcgtt cggccttttt ataacaaggt
     8281 tgttctggcg cgtcttcata atgggagcgg ctcggagatt cgaatactat actcggaaga
     8341 ctcttttcga gaaacttctt acacttcccc ctacgttcta cgacaagacc agagtgggcg
     8401 acctgatggc cagattcaca aacgatgtca acgccgttcg aatggctatg ggcccggcag
     8461 ttgtaatgat catagatgct gtctttctgg ttaccgtcac aatcttcgcc atggggctct
     8521 tcacgaactg gagtttgacc tggatctcga ttgcccctct tccggctctc gcaattgttt
     8581 ctacgttttt cggaaagatg atccataaga gattcaaatt ggttcaggcc tccttttccg
     8641 atctaaccga tacgatagag gaatcgattt cgggtgtgcg cctgataaaa agctacggta
     8701 tagaagacct tagaaacgag actctcacgg aaaagtcgaa tctctacgta gacaagaata
     8761 tgaaactggt caaagtctgg ggaatgttct tcccgttgac tcagacccta tcgatgctgg
     8821 gctctgtact ggccacgcta ttcggtggac gaatggtaat actcgggcag ataactctgg
     8881 gacagtacat aacattcaca tcgtatcttg gaatgcttgt ctggcctatg accgcgtttg
     8941 gatgggttat aaacatcatc cagagaggaa gggcgtccta cagaaggctc atggaagtcc
     9001 ttgaagaaga gaacaatatt actacgaacg atccagtcga agtcggcagc ttcgagggcc
     9061 acgtcgtgat gaagaacctg accttcacct atccaaccgg cagtagaccg gcgctggaaa
     9121 acattgattt cgaagttaaa ccgggctcca agattgccat agtaggaacg acgggatccg
     9181 gcaaatcgac aatggcaaag cttatagcca ggctgtatcc cgttgaagac gggatgcttt
     9241 ttatcgacgg tgtagatatc aacaatctta gacccgaaac gattagagac aacgttgcat
     9301 atgttccaca ggagacattc ctcttttcag agacagtagg aaacaacatt cgattcggaa
     9361 atatggagat ctcggatgaa gaggtcgaaa ggtacgctaa ggttgcctcg attcatgaag
     9421 atattgaagg ttttagcaag ggatacaaaa ccattgtcgg agagagaggc gtaacgcttt
     9481 ccggaggtca aaaacagaga atctcgattg caagagccct tataaaggag gcgcctctcg
     9541 taattcttga cgactgtctt tctgccgtag ataccgagac agaggcagag atcttgaagt
     9601 cccttaaaga gtcggaaacc aacaggtcga taatcgttat ttcccacagg ctgaaggccg
     9661 ttgtggattc cgacgagata tacgttctcc acgacgggag aatcgtggaa aagggagtcc
     9721 acacgcagct cgtagaggcc agagggttgt atcagagaat gtacgagaga cagatgctcg
     9781 aagaaaagct cgaggaggaa tagtcgtggc agaaaccgtg tacagcgaag tagaagaaaa
     9841 actcaaagtt gaagactgga gggtagtctt tagactttgg aagtacacaa agccatacgt
     9901 tcctattctt attctcgcat tgcttttaat cgctgcctcg actgctctcg acctactgcc
     9961 tccactgctt atgagaaagg caatagacga ttacatggac aatcagtatt ctctcgtcgt
    10021 tgtcgaggag ggtgattcat acaatttcgt ccagtcggaa gagggtacct tttatcttca
    10081 tcaggatgag tctggaatgt acagcgtcac cgacgggacg accacttatc caattaccga
    10141 aaagcagctg gccgaactaa gagttgagga tctcaacggt attggacaga tcgcaatttt
    10201 catgattctg attctctttt cgctcttctt tgccaattat ggacaggtct acgcgacatc
    10261 ttacatgggg cagaagatca cacacggaat tagagtcgat ctctttcaac acctgatcag
    10321 agtccctatg agattcttcg ataccaaccc cagcggaagg cttgccacaa gggtggcaaa
    10381 cgatacacag aatcttgcgg agttcttcac cagtgttata acatctatgg tgaaggatgt
    10441 tctcttactc ggcggaataa tcgtaataat gctcaacctc tctacgtatc tcggcttgat
    10501 aacggtggca atcctcccgg tgattgccgg cgcgatcttt gtcttcagat acttcgatag
    10561 agtagcgtac agaaaggtga gaacccggct ggcaataatc aatgcattcc ttgccgaaca
    10621 catctccggg atgtccataa cgcagctttt caatcaagaa gagaggaaga agaaggagtt
    10681 cgacaccgtc aataagagcc attttaagag ccttatggag cagctatggg tcttcgcaat
    10741 tttcagaccg ctgcttgacc tcctctacta cgtaacgata gcgattgtta tttggttcgg
    10801 ggtaaagagc attattggaa acactcttgc cttcggagta ctggtcgcct tcatctcata
    10861 tattgacatg ttcttccggc cacttcaaga cattgcagag aagtacgaca tcgtgcagaa
    10921 tgcaatggcc tctgcagaga agatcttcaa gttgatggac gaagaggaag agaaatataa
    10981 ctcgaacgag ccaggagtca aatcactcga aggcacggta gagtttgaaa atgtaacttt
    11041 cgcatacgac ggcgagcaca aagtcttgaa ggatataagc ttttcggttc gtccaaaaga
    11101 aaagatcgcc ttcgtcggag aaaccggtgc gggaaagact tcaataataa gtatcctcac
    11161 agggctttac aagattcagt cggggaagat aaagatagat ggcaggaaca tctacgatta
    11221 caaccttcag agctttcgaa agaaagtcgg cgttgttctc caggatgtct tcctcttttc
    11281 cgggagtatt ctcgacaaca tacgcctctt cgatgagagc ataagcaggg aaaaagtgat
    11341 cgaagcggca aagtacgttt atgcacacca tttcattgag cgtttgcaag acaagtacga
    11401 cacagttatc ctggagagag gcggtacgct ttcggcaggt gaaaggcagc tgatagctct
    11461 tgcaagggct gtagttttca atgctgatat tcttgtcctc gatgaagcca ctgcaaacgt
    11521 cgacacggaa acagaggcgc tcattcagag gg
//
LOCUS       DAOQYI010000047        10981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_6405_length_10981_cov_81.511349, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000047 DAOQYI010000000
VERSION     DAOQYI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6405_length_10981_cov_81.511349"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1063)
                     /locus_tag="PKL93_09255"
     CDS             complement(206..1063)
                     /locus_tag="PKL93_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ71483.1"
                     /translation="MNDVIQTIIEWAIRIGVSIAILLVARWLSGLFYKAFIKFTEKTS
                     VVSVQYRKTMRTLFNLAFYALAAFIIISVLFKNLAPVLAGLGVSGIIVGLAVKEPLEN
                     LISGILIMINKLIYEGEAVDIGGTSGGVQEIKLNHVLIKTWDGKLVTIPSKNVWAATI
                     IHFWPENIRRNDLNVGVSYSSDLNKVMKILEEAVNSYEKLYVDDNHKPMIHFTGYGAS
                     SIDFVVRFWVEKANFVDSSTELAKIIKSRFDENGIEIPFSQLDLHIKDGPIEGIKLSN
                     KERDAETSS"
     gene            1315..2220
                     /locus_tag="PKL93_09260"
     CDS             1315..2220
                     /locus_tag="PKL93_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71484.1"
                     /translation="MKRLILIGLLFIAFSGFALNGPITVASKIDTEGALLGQMIVIVL
                     EKNGFEVVDKTEFGTTSVIRKAITAGEIDIYPEYTGNGGFFFDDTDPTVWKNAKSGYE
                     TVKTLDFERNGLVWLTPAPANNTWALAIRKDLSDGEGIKTLEDLANYVNGGGFIKLAA
                     SEEFLTRADAMPAFQEAYGFELSNDQLLAFSGGNTAQTIRAAAQNIDGVNLAMAYGTD
                     GALSALRLVVLEDTKGVQPVYEPAPIVRKKIFEMYPEIGELLKPVFESLDLETLQSLN
                     ASIAIEGLDAGDVAEQYLKSKSLID"
     gene            2280..3401
                     /locus_tag="PKL93_09265"
     CDS             2280..3401
                     /locus_tag="PKL93_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71485.1"
                     /translation="MLLSILLPGFLSVKETRISAALPVSLASFSVIGLVVLLAVSGVT
                     ILLSFGGYYFLMGLAGGLAPVYGFFLAGSSAVQIASETGPFARASPSIGLWILLLAGY
                     VVVFAARRKLKDKRILSSLLVLIPIASFAVLLGSGTLRELAIMKEYANRSDRFLEEMA
                     RHLLIAGGAVVAGVIIGVPLGVAANRSPKVEKPVFGVVNFIQTIPSIALFGLLIAPLS
                     YLSRSLPFLRELGITGVGWAPAMIALVLYSLLPIVRNTYSSLKVIPADTVEAARAMGM
                     SRLQVLGKVEIPISLPVVLAGVRTAAVQAVGNTTMAALIGAGGLGVFVFQGLGQAAMD
                     LVLLGALPIVALALIVDSLMQLLIAILTPRGLVSEVADD"
     gene            3394..4488
                     /locus_tag="PKL93_09270"
     CDS             3394..4488
                     /locus_tag="PKL93_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71486.1"
                     /translation="MIRFENVTKEYDEETVAVRDITFEIPSRELTVLIGPSGCGKTTT
                     LKMINRIVKPSGGRILIDGEDVKDMNVKSLRRGIGYVIQNVGLFPHMSVKDNIATVPK
                     LLKWERSKIERRVSELLELVGLEPKSYANKLPKELSGGEAQRVGVARALAADPPIILM
                     DEPFGAVDPLTRVRLQDEFARIQEELHKTIVFVTHDIDEAIRLADRIAVMKAGRILQL
                     DTPEEILARPKEKFVHDFIGSDRALKRLSRIPVMEYIEKCETIEIDGGWDQLGMTKEE
                     FIWALDEDRKLVGWVDVSHSNRELPLREALTEMLEEEILSPDSTVKEALSRMLESGTV
                     ALPVVERSGRFLGQISLSKIQELTRSDREE"
     gene            4472..5215
                     /locus_tag="PKL93_09275"
     CDS             4472..5215
                     /locus_tag="PKL93_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71487.1"
                     /translation="MTGKNSRLSSWIFLGIFLLAFILLISNMALWENFLGWLFPEERE
                     VLHPRGTLLELVAEHMWMVLVSSGLATIIGISIGVLVTRPMGRQYLPLVSNLSSLGQT
                     FPPVAVLALAVPLLGFGFKPTVAALLLYGLLPIVRNTIAGIENIPPDVREAAYGMGMS
                     SWQALFKVEFPLALKVIMSGIRISVVINIGTATVGATIGAGGLGSPIIAGLVAENPAF
                     VLEGAIPAALLAFSVDAFLGNLEKTISSG"
     gene            complement(5234..6562)
                     /locus_tag="PKL93_09280"
     CDS             complement(5234..6562)
                     /locus_tag="PKL93_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ71488.1"
                     /translation="MIDIFAGWFSALGLSETFDLVLANIVVGIIMVVVAFLSKFLFEK
                     LLIKPIEIIVRKSAFKWDDYLIKHRLLYKIALMIPAIVIALFARAFPAIEDLIYKLVA
                     TYLIFIVAVVIDSFLDVLTSAYQSLETSKDKPIKSYITVVKIMVYIVAGVIAVSVLTG
                     ISPWGILSGIGAMTAVLLVVFRDSLLGLVASIQISKNNLVSIGDWIEVPKFQADGDVI
                     DITLTTIRIQNWDKTITTIPSYAIISESFKNWKGMFQAGGRRIKRSVFIDTSSIRFLD
                     DELYERLYRIEILRPYLESKKKEIEEFNKKNNVDVSEPVNGRKMTNIGTFRAYLMAYL
                     RNLPGTNKNMIIMVRQLQPTDTGLPLEIYAFSSDTSWVNYEALQSDIFDHVFAALPHF
                     GLRVFQNPTGRDVRALGELFVSGKMAVPHPSAGEKSATINNDEDRQIDYH"
     gene            6729..7187
                     /locus_tag="PKL93_09285"
     CDS             6729..7187
                     /locus_tag="PKL93_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71489.1"
                     /translation="MSELKCPECYSSDNRATPLKNPEDCLSNHVQYICSTCGRAICME
                     GDEREKHGPLSSFGSFNDAMLYLRAAEALFNGPCGIYELTDGAKVFYKIFKNKDGLMN
                     YLMENPEKRCPLGEALHKTEEFRPVVEGQIRRLDKDEVEEYLREKEEVDD"
     gene            7180..8442
                     /locus_tag="PKL93_09290"
     CDS             7180..8442
                     /locus_tag="PKL93_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNQ71490.1"
                     /translation="MTSFETVFILVVYGIAFFELFLLSLNFRNAKLSKVSLPDNLRDV
                     FSDDLIEKSVEYSRAKSLFVIVSSVVNLVALSIGIFWGFGVIESLSVKLAEGFALRGL
                     IFFSLIASIYFFISLPFSIYSTFVLENRYGFNRTTPKTFVSDKIKEILLAAGIGLPLV
                     YLALLAIDSFEYWWVYLLIGVVGFEILTQLIFPTVILPLFYKLKPLEDENLAKRIREI
                     ADKAGFGVKSILVMDASRKTRHTNAFFTGIGRAKRIVLYDSLLEKHSSEEIEAIFAHE
                     AGHFKRKHILKGMLISNAVAVLAVVLLWMIVESDTVAGIFGVSEKYAILLYAGIFLSS
                     IFTVLDWIDSFISRKWEFEADSYSAMITGDTQPMIRALKNLSVSNLSNLSPHPLYAAL
                     YYSHPPSWERIEKLNGIVSNIDERKSRG"
     gene            8447..9409
                     /locus_tag="PKL93_09295"
     CDS             8447..9409
                     /locus_tag="PKL93_09295"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNQ71491.1"
                     /translation="MKLKFINGVTPVNRLEWIEEYLGLPFELFCKHDELTGFITSGNK
                     IRKLEYLLKDALEKKADTVFTCGGIQSNHCRATAMAARSLGMQPVLFLRGRPMEIPQG
                     NVLLDTMVGSDIHYVTKEEYSRIDEIFARKKEEYENKGRKVYLIPEGGSNALGARGYV
                     DAVKELSGQINLDGVEAIFTAVGSAGTYAGILAGLRILGYNTGVIGINVTKDPSSIFV
                     EKTKRLIGEMREYEIDVSIDDGEIEIVDDFSGPAYAVPSEEDIELIKSLARERAFFLD
                     PVYTAKAFRGMLQISRERFAGKRVVFIHTGGLFKLFDNPATYVK"
     gene            complement(9429..9791)
                     /locus_tag="PKL93_09300"
     CDS             complement(9429..9791)
                     /locus_tag="PKL93_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ71492.1"
                     /translation="MRNMRGRRMGMGRSWIELYLLLLIAEKPAHGYELSSRINDFEIP
                     IFGVGQMGSLYRVLGSLEEMGLITSEWDTEDAGPAKKNYKITRAGLEYLKTAEVKIKR
                     FRENVDKFLDRLNNLNKI"
     gene            complement(9812..10183)
                     /locus_tag="PKL93_09305"
     CDS             complement(9812..10183)
                     /locus_tag="PKL93_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNQ71493.1"
                     /translation="MKIAIPTIDNKGLQSHISEHFGHSPFFAFVTVENNEIVSHEIEV
                     NPFEQHEPGEIPGYVKSKGADVIITRGMGGRARQFFETLGVHAITGASGTVEELVKTY
                     IEGELSSIEYEPEDHGKFHNH"
     gene            10351..10707
                     /locus_tag="PKL93_09310"
     CDS             10351..10707
                     /locus_tag="PKL93_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71494.1"
                     /translation="MKKTLAFLIIAALCVTALANGLLVVTLKDGSVYEFYLDEIESLV
                     YVAEDELPLEPKYMSFEKLVFVPGEEINLYFFGTSGLGLTPGSESFLRILRMEASRKT
                     TCTTFSMLTSVAWQTV"
     gene            10906..10981
                     /locus_tag="PKL93_09315"
     tRNA            10906..10981
                     /locus_tag="PKL93_09315"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10939..10941,aa:Pro,seq:ggg)
BASE COUNT         2689 a         2562 c         2885 g         2845 t
ORIGIN      
        1 acccgcaacc gtggtcttca caagagcaga tcccgaaaca agttcgggat gacagtgtat
       61 gaagatttca aaacgtcgca tcccgccatt ctgaactcca ttgagaatct cggactttga
      121 taggatcaaa atctacaaag atgtgagagt tgtgaggagg atagaggtat gaagatcaat
      181 atcgttcaga gaacctcaca atccatcatg aacttgtttc agcatcgcgc tccttatttg
      241 acagctttat gccttctatg gggccgtcct ttatatggag atcgagctgg ctgaaaggaa
      301 tctctatgcc gttttcgtcg aatctagatt ttatgatctt tgccagttcc gtgcttgagt
      361 ccacgaagtt cgccttctct acccagaatc taacgacgaa gtctatggaa gatgcgccgt
      421 atccggtgaa atgtatcatt ggcttgtggt tgtcatcgac gtaaagcttc tcgtaagaat
      481 ttacggcttc ctccaggatc ttcatgactt tgttgaggtc gctcgaatag gaaaccccga
      541 cgttgagatc gtttcgtctt atgttttcgg gccagaagtg aataatcgtt gctgcccaga
      601 cgtttttgct ggggatcgtg actagctttc cgtcccatgt ctttatgagc acgtgattga
      661 gtttgatctc ctgaactcct ccggatgtcc ctccgatgtc aactgcctcg ccctcgtaga
      721 ttagcttgtt tatcattatt agtatcccgc tgatcaggtt ttccagaggc tccttgacag
      781 cgagaccgac aatgataccg gatactccga gaccggcgag aacgggagcc agattcttga
      841 agagaaccga gataattatg aaggccgcca gagcatagaa ggcgaggttg aacagcgttc
      901 tcattgtctt gcgatattga acggagacaa cggatgtctt ctcagtgaat ttaatgaatg
      961 ctttgtagaa gaggcccgaa agccatcttg ccaccagaag gattgcgatg ctgactccga
     1021 ttctaatcgc ccattcgatt attgtttgga ttacatcatt catgattttc cctccttctt
     1081 ccatgataac tattcttaca taagatctgg ccgaagccaa gtaggaacgg acccatcgct
     1141 ttgacctgaa aaagggatct tgtctgtgag gcacagtgcc tttacaaaga tcagcatttc
     1201 gttgagtcga attgtttaga tacactccaa aatacttttc aaagatattt gaaggtagtg
     1261 tacaattaaa tggagtataa ttatcggcaa taaaaatgat ggaggttgtt tattgtgaag
     1321 agattgattc taattggatt attgttcata gcattttcag gttttgcgct caatgggccg
     1381 attactgtgg catctaagat agatacggaa ggcgcgctcc tgggccagat gattgtgata
     1441 gttctcgaga agaatggatt cgaggttgta gacaagaccg aattcggtac gacgagcgtc
     1501 attcgaaagg cgatcactgc cggagaaata gatatctacc ccgagtacac gggcaacggt
     1561 gggttcttct tcgatgacac cgatcccacg gtatggaaga acgctaaatc cggatatgag
     1621 acggtgaaga cgctcgattt tgaaaggaac ggactcgttt ggcttacccc cgccccggca
     1681 aacaatacct gggctctggc tatccgaaag gatctttccg acggtgaagg aataaagact
     1741 ctcgaggacc ttgcgaacta tgtgaacggt ggaggcttca tcaagcttgc cgcttccgag
     1801 gagtttctga cgagagccga tgcgatgcca gccttccagg aggcatatgg ctttgagctt
     1861 tccaacgatc agcttctggc cttctccgga ggaaacacag ctcagaccat cagagcagca
     1921 gcgcaaaaca tcgacggggt aaatctggct atggcctacg gtacagatgg agccctctcg
     1981 gctctgaggc tggttgtcct tgaagatacc aaaggcgtgc agccagtata tgaacctgcc
     2041 ccgatagtga gaaaaaagat cttcgagatg taccccgaga tcggagaact gctaaagccg
     2101 gtcttcgaat ctctcgatct ggagacgctt cagtccttga acgcttcgat agcgattgag
     2161 ggtctggacg cgggcgacgt agccgaacag tatctcaagt cgaagtcttt gatagattaa
     2221 agcctatggc gtcctttgcg cgacgaaaag acatgatggc cattcttgga gtggccatca
     2281 tgttgctttc tatactgctt ccaggttttc tttcggtgaa ggagacgaga atttccgcag
     2341 ccttgccggt cagcctggcc agcttttccg taattggcct cgtggttctt ctggcagtct
     2401 ccggtgtcac catcttgctt tctttcggcg gatactactt cttaatgggc cttgcaggtg
     2461 gactcgcccc ggtatacggt ttcttccttg ccggtagcag cgcggttcaa atcgcctcgg
     2521 aaactgggcc gtttgccaga gcgtcccctt ccatcgggtt gtggattctc cttctggccg
     2581 gttatgtggt cgtctttgcg gcaagaagga aactgaaaga caagagaata ctgtcatcac
     2641 tgctagttct gatcccaatc gcttcgttcg cagttcttct cggctccggt actctcaggg
     2701 agctagcgat aatgaaggag tacgcaaaca ggagcgacag gtttctcgaa gagatggcaa
     2761 ggcatctctt gatcgccggc ggagccgtag tggcaggagt gataatcgga gtgccccttg
     2821 gagtggcggc caacagatcg ccaaaggtcg agaaaccagt ctttggtgtg gtgaatttca
     2881 tacagacgat tccgagcata gcgcttttcg ggcttctcat agcgccgctg tcgtatctct
     2941 ctcggagtct tcccttcctg agggagctcg ggataacggg agtcggctgg gcgccagcga
     3001 tgatagccct cgttctctat tcactccttc ctatcgttag aaatacttac tcgagtctga
     3061 aagttattcc ggccgatact gtggaagccg ccagggcaat gggaatgagc agattgcagg
     3121 tccttggaaa ggtggagatt ccgatctcgc tgccggtagt tcttgcagga gtaagaacgg
     3181 cggccgtcca ggcggtgggc aatacgacga tggccgcgct cattggagcc gggggtcttg
     3241 gggtcttcgt ttttcagggt cttggccagg ccgccatgga tctcgtactt ctcggagcgt
     3301 tgccgatcgt ggcgctcgcc ctgatagtcg attctcttat gcaattgctg atagctattc
     3361 tgacgcccag agggctcgtt tcggaggtgg ccgatgatta gattcgagaa tgtcacgaag
     3421 gaatacgatg aagagactgt agctgtgaga gatataacat ttgaaatacc ctcgagagag
     3481 ctcactgttc ttattgggcc ttctgggtgc ggtaagacga cgactctcaa gatgataaac
     3541 agaatcgtta aacctagcgg cggcaggatc ctcatcgatg gagaagatgt gaaggatatg
     3601 aacgtcaaga gtctaagaag agggataggc tacgtaattc agaacgtggg tctctttccc
     3661 cacatgtccg tgaaggacaa tatcgcgacc gtgcccaaac tgctgaaatg ggaaagatcg
     3721 aagatagaga gaagggtaag cgagctgctc gaattagtcg gactggagcc gaaatcttat
     3781 gcaaacaagc tcccaaaaga gctttcggga ggggaggccc agagagtggg agtcgcaaga
     3841 gcgcttgcgg ccgacccgcc gataatcctt atggatgaac ctttcggggc ggtcgatccc
     3901 ctcacgaggg ttagactgca ggacgagttc gccaggattc aggaggagct tcataagacg
     3961 atagtcttcg taacccatga tatagacgag gcgataagac ttgccgacag aattgcggtc
     4021 atgaaagccg gaaggatact tcaactcgat accccagaag agattcttgc aagacctaaa
     4081 gagaagttcg ttcacgattt cattgggtcc gacagggccc taaagaggct ctccagaata
     4141 ccggtcatgg aatacataga aaagtgcgag actatagaga ttgacggcgg ctgggaccag
     4201 ctgggtatga cgaaagagga gttcatctgg gcgctggacg aagataggaa actcgtcggt
     4261 tgggtggatg tttcgcattc aaacagggag cttcctctca gggaggcatt gacggagatg
     4321 ctcgaggagg agatcttgag ccccgacagc actgtcaaag aggcgctttc caggatgctt
     4381 gagagcggta cggtggcgct ccccgtcgtg gagagatcgg gacgctttct cggtcagata
     4441 agcctatcga agattcagga gttgacgaga agtgacaggg aagaatagca gactttcgtc
     4501 atggatcttt ctggggatct ttctgctggc gttcattctc ctcatttcca acatggcttt
     4561 atgggagaac tttctaggct ggctctttcc ggaagagagg gaggtcctcc acccgagagg
     4621 aaccctgctt gaactcgtgg cagaacatat gtggatggtc cttgtctcga gcggacttgc
     4681 cacgattatc ggcatatcga ttggggtatt ggtcacgcgg ccgatgggga gacagtatct
     4741 gccgcttgtg tcgaacctga gctctcttgg ccagaccttt ccgcccgtcg cagtgctggc
     4801 tctcgccgtt cccctactcg gctttggatt caagccgaca gtggccgctc tgcttctcta
     4861 cggacttctg cctatagtga ggaacacgat tgccggcatc gagaacattc ctcccgatgt
     4921 tcgcgaagcc gcatacggta tggggatgtc ttcatggcag gcgctgttca aagtggaatt
     4981 cccacttgcg ctgaaggtca taatgtcggg aataaggata tccgtggtga taaacatcgg
     5041 cacagcgact gtcggtgcaa cgataggggc aggagggttg ggatcgccca tcatcgcagg
     5101 actcgttgcg gagaatcccg cctttgtgct cgagggggct attccggcgg ctcttctcgc
     5161 cttttcggta gacgcatttc tggggaattt agagaagact atatcgtcgg gatgactgcg
     5221 gacctgtcgt taactaatga taatctattt gccggtcttc gtcgttgttt atagtggcgg
     5281 atttctctcc ggcagacggg tgcggaacag ccatcttgcc gcttacgaaa agttcgccca
     5341 gagccctcac atctcttccc gtaggattct ggaagacccg gagaccgaag tgtggcagtg
     5401 cggcgaagac gtgatcgaag atgtccgact gcaaagcttc gtagttcacc caagaagtat
     5461 cgctgctgaa ggcgtatatt tccagaggaa gcccggtgtc ggttggctga agctgcctta
     5521 ccatgattat catgttcttg ttcgttcccg ggagatttct cagatatgcc atgagatagg
     5581 ccctgaaggt tccaatgttg gtcattttcc tgccgttgac gggttcgctc acatctacat
     5641 tgtttttctt gttgaattcc tctatctctt tctttttgct ttccagatag ggtctgagga
     5701 tctcgatcct gtaaagcctt tcgtaaagct cgtcgtcgag aaagcgaatc gacgaggtgt
     5761 caatgaagac cgatctcttt atccttcttc ctccggcctg aaacattccc ttccagttct
     5821 tgaaggattc cgagattatc gcgtaggagg ggatggtggt gatcgttttg tcccagttct
     5881 ggatcctgat tgtggtaagc gttatgtcta taacgtctcc gtcggcctga aatttcggaa
     5941 cctctatcca gtcgcctatg ctgacgagat tgttcttcga aatctgtatg cttgcgacga
     6001 gtcctagaag cgagtcgcgg aagactacca gcagaactgc cgtcatcgct ccgatcccgc
     6061 taaggattcc ccatggagag attccagtca gcactgatac cgcaataacc ccggccacaa
     6121 tgtagaccat gatcttcact acggtaatat agctctttat aggtttgtct tttgaggttt
     6181 caagggactg ataggcgctc gtcaagacgt ccaggaagga gtctatcacg acggcgacga
     6241 tgaatattag atacgtggcg accagtttgt agatcaagtc ctctatcgcg gggaatgccc
     6301 gggcgaagag agcgataact attgccggta tcatcagtgc tatcttgtag agaagtctgt
     6361 gcttgattaa ataatcgtcc cacttgaacg cggactttct cacaatgatt tctatgggct
     6421 tgatcagcag cttctcgaag aggaatttcg agagaaaggc cacgactacc attattattc
     6481 cgacaactat attggcaagt accaagtcga aagtctcgct gagtccgagc gccgaaaacc
     6541 acccggcaaa gatgtctatc acctgatcgc ctccagagac aatgataaca tcactggatc
     6601 gacacagcta cttttacggc gttctgggta agtctgattc ttgatagttg cgggagtctt
     6661 ccaccaaaca ttcgtcggta cagagggtta cagagatcgc cacaatgtcg taaaatagtg
     6721 tattggaggt gagtgagttg aaatgtcccg aatgctactc tagtgataac agggcgacgc
     6781 cgcttaagaa tcccgaagac tgcctgtcga accatgttca atacatctgt tccacttgtg
     6841 gcagggcgat ttgcatggag ggcgatgaaa gagaaaaaca cggtccctta tcctccttcg
     6901 gttctttcaa tgacgcaatg ctttacctcc gggcagccga ggcattattc aacgggcctt
     6961 gcggcatcta cgaattgact gacggtgcga aagtcttcta taagatcttt aagaataaag
     7021 acggactgat gaactaccta atggagaacc cggagaagag gtgtcctttg ggagaagcgc
     7081 ttcacaagac agaggaattc aggccggtgg ttgaagggca gatccggaga ctggacaagg
     7141 acgaggtcga agagtatctg agagagaaag aagaagttga tgactagctt cgagacagtc
     7201 tttattctcg ttgtctatgg aatcgctttc ttcgaactct ttttgctgtc cctaaacttc
     7261 agaaatgcga aactctcgaa ggtctcactt ccagacaact tgcgagacgt cttcagcgat
     7321 gatctcatag aaaagtctgt ggaatattca agggcgaaga gtctttttgt tatcgtttcc
     7381 tctgttgtca accttgtagc actcagcata ggaatcttct ggggattcgg agtaatcgaa
     7441 tctctttcgg tgaagttggc cgaaggtttt gcactgcgtg gcctcatctt tttctccctg
     7501 atcgcctcga tttacttttt tatctctctc cccttttcga tatactcgac tttcgtcctg
     7561 gagaacagat atggattcaa tcgaacaaca ccgaaaacat tcgtctcgga caagataaaa
     7621 gagatccttc ttgcggctgg gatcggcctc ccactcgtat atcttgccct ccttgcgatt
     7681 gattccttcg aatactggtg ggtctatctg ctgataggcg tcgttggatt cgagatactt
     7741 actcagttga tattcccgac agtgatcctg ccgcttttct acaaactgaa gccgcttgaa
     7801 gatgaaaacc ttgcaaaaag gataagagaa attgcggaca aggcaggatt cggagtcaag
     7861 tcgatccttg tcatggacgc ctcgaggaag acgcgccaca caaacgcctt tttcacgggt
     7921 ataggcaggg caaagagaat tgttctgtac gacagcctcc tggaaaagca cagttcagag
     7981 gagatagagg cgatcttcgc ccacgaggcc ggccacttca agcgtaaaca cattctgaag
     8041 gggatgctca tatccaacgc ggtcgcggtc ttagcagtgg ttcttctgtg gatgatagtc
     8101 gaaagcgata ccgtcgccgg tattttcggt gtctcagaga aatacgcgat tctgctttat
     8161 gccggtatct tcctgtcttc gatctttacg gttctggact ggatagactc gttcataagc
     8221 agaaagtggg aattcgaggc cgacagttat tcggcaatga ttacaggcga tacccaaccg
     8281 atgataagag ctttgaagaa cctatccgta tctaatttgt ctaacctcag cccgcatcct
     8341 ttgtatgcag cactatatta ctcgcatccg ccctcgtggg agcgaattga aaaactcaac
     8401 ggaatagtct ctaatataga tgaaagaaag agtagggggt gagaacttga aactgaagtt
     8461 cattaacggt gttacgcccg tgaacaggct tgaatggatc gaagagtacc ttggccttcc
     8521 attcgaactc ttctgcaagc acgacgagtt gaccggcttc atcacgagcg gcaacaaaat
     8581 cagaaagctc gagtatcttc tgaaagatgc tctggaaaag aaggcagaca ccgtcttcac
     8641 ctgcggggga attcagtcga accactgcag ggcgacggcg atggcggcga gatcgctcgg
     8701 aatgcagccg gtactcttcc tcagggggag gccgatggag attcctcaag ggaacgtcct
     8761 tttagatacc atggtgggaa gcgacatcca ttatgtgacc aaggaggagt attcgagaat
     8821 agacgagatc ttcgccagga agaaggaaga gtacgagaat aagggaagga aggtttacct
     8881 gatcccggag ggcggttcaa acgcgctggg agcgagaggt tacgtggacg ccgtgaaaga
     8941 actctctggc cagatcaatc tcgacggagt tgaagccatc ttcactgcag ttggcagcgc
     9001 cggaacctat gcgggaattc tggcagggct aagaatactt ggctacaaca ccggagtcat
     9061 aggaataaac gttacgaagg acccttcttc aattttcgtg gaaaagacaa agagactaat
     9121 aggcgagatg agggagtacg aaatagacgt atcgatagat gacggcgaga tcgagatagt
     9181 cgacgacttc tcgggccctg cttatgccgt gccttcggaa gaggatatag agcttataaa
     9241 gagccttgcc agggagagag ccttcttcct tgaccccgtc tatacggcga aagccttcag
     9301 gggaatgctt cagatctcaa gggaaaggtt tgccgggaag agagttgttt tcatccatac
     9361 tggtggattg ttcaagctgt tcgacaatcc ggccacttat gtaaagtaaa gccctgcatc
     9421 gcagggcttt atattttatt caggttgttc agtctgtcaa gaaacttgtc gacgttctcc
     9481 ctgaatctct ttattttgac ctccgctgtt ttcaggtatt cgaggccggc ccttgtgatc
     9541 ttgtagttct tctttgccgg gcctgcgtct tcagtatccc attcggaagt tatgagtccc
     9601 atttcttcca ggctgcccaa cacacgatag aggcttccca tttgaccgac accaaagatc
     9661 ggtatctcga aatcgttgat tctagaagaa agctcataac catgcgcagg cttttcggcg
     9721 atgagaagaa gcagatacag ttcaatccag gatctgccca tccccattct tcttcccctc
     9781 atatttctca tgccgtactc catgtcatta attagtgatt gtggaacttc ccgtggtctt
     9841 cgggctcgta ttcaatactg gaaagttcac cttcgatgta agtttttacg agctcttcta
     9901 cagtcccact ggctcctgta atcgcatgta cgccaagagt ttcgaagaac tgcctcgccc
     9961 ggccgcccat gcctctcgtt atgatcacat cggcgccttt actcttcaca taacccggaa
    10021 tttcaccagg ctcgtgctgt tcaaagggat ttacctcgat ttcgtgagag acaatttcgt
    10081 tgttctcaac agtcacgaat gcaaagaacg gagagtgacc gaaatgctcc gatatatgtg
    10141 actggagtcc tttgttatca attgttggaa tagctatctt cacaaaccaa cctccttgca
    10201 tatattcaac taccatatat tatagcatct gaattggact ggattctgac atcatcgacg
    10261 atctaggttc aactgtcatt ttaggaaggc acaaaacagc agccctccgc ttaagcaatt
    10321 gtgagagaat cttcatggat gaggtccggc atgaagaaga cgcttgcttt tttgataatt
    10381 gcggcattgt gtgttactgc gcttgcgaat ggtttgctgg tcgtaacttt gaaggacggt
    10441 tcagtctacg aattctacct cgatgaaatc gagagtctag tatacgttgc ggaagacgaa
    10501 ttgccccttg aacctaagta catgagtttc gaaaaacttg ttttcgttcc cggtgaagag
    10561 atcaatctct acttctttgg aacttcggga ctggggctta cgcctggatc ggaatcgttc
    10621 cttcggatat tacgcatgga agcgagtcgg aaaacgacct gcacgacatt cagtatgctt
    10681 acgtcggtgg catggcagac ggtttgatag tattcaccgc tccatgggat gccggattct
    10741 acgacttcag gttgaatgat tccgagagcg gcggcatcga gatcgacttc ctgaccttcg
    10801 aggtccgaga gtagcctgca gagccaattt taattatcct aaagttgccc tgggcgtcgt
    10861 tctgctcttg aaaggttatc ccaactgtga taaaatcttc gagtacgggg catagcgcag
    10921 ttggaagcgt gtctgcatgg ggcgcagaag gtcgctggtt caagtccagt tgccccgacc
    10981 a
//
LOCUS       DAOQYI010000048        10820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_6541_length_10820_cov_72.960334, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000048 DAOQYI010000000
VERSION     DAOQYI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6541_length_10820_cov_72.960334"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKL93_09320"
     CDS             <1..272
                     /locus_tag="PKL93_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71495.1"
                     /translation="HDAENGVSITGYGSCEQNWLTIQGFAATEEAIPTSAFGSPLLCH
                     PELVSGSVPRTKNPDMYRRRTRILGGDGRPLTGNVLFMSVQRFLG"
     gene            836..2401
                     /locus_tag="PKL93_09325"
     CDS             836..2401
                     /locus_tag="PKL93_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ71496.1"
                     /translation="MTEKRSIKEIMKAYSEVPDKDFRQKLKILNELSQTMFKEGNPLL
                     ASETVKAELSRIEAPACIELANITHNMIKYSCVVGNYDSSIEYALKAVVLFKEFGTAD
                     DVNDVIGNIGGVYVYMGRFEESLEYTDKALEYVRIKGDDLAVANFLNNKAVALKSLER
                     FEEAVDCLIKAIEIKLSLGKKADLCNSYFNLSEVLISAERYDEVPAIFKKTEPLVKEI
                     EDPAGSAEFDFLKAQYYMGTGNYAKALEKIESYIEYQKESGSMSKVPGALRNKIKIHE
                     ELGEEGLALETYRELDLLSQRTYKETCSARAAELAASFRFQQKMHEIQLLSNQNLELQ
                     EAKTTIEKQYEELLEIDSKLKKANELLEKQAEIDPLTGLLNQKKMYPIIEREINRATR
                     YGTPLSMIMIDLDNFKNVNDTYGHLQGDILLKNVAGIIRSSLRKVDFVFRYGGEEFLV
                     LLPSSDLQMASSTADRLRDELAKTTYPKVTMSAGVSSWSGEDATHFIQKTDHLLYAAK
                     GKGKNRVEFQKGS"
     gene            complement(2493..3224)
                     /locus_tag="PKL93_09330"
     CDS             complement(2493..3224)
                     /locus_tag="PKL93_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71497.1"
                     /translation="MSGVAYWTILMKDMKNYYLKPPNISWGIVFPISWTLMFFVRSGS
                     DVEIRDILPGVMALSVLFGTTSMLAVTITFERKSRSFERLLLAPISLKLLMLAKTTGA
                     ILFGIINAFVPVLFALLIVDLSGINWFLVSVSVLLIAVTSTFLGLFIAVSASEVFEAQ
                     TFSNFFRFPMMFLCGLFIPIENLPAFLRPFSFVLPLTYGADSLKHAINRNGIIDPLAS
                     VFILLGFAALLFLISTRNIERRWIY"
     gene            complement(3221..4186)
                     /locus_tag="PKL93_09335"
     CDS             complement(3221..4186)
                     /locus_tag="PKL93_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71498.1"
                     /translation="MGIIEVSGLSKNYNGFTAVKSIDFSVEKGEVFGFLGPNGAGKTT
                     TINMLTGLARPTSGRIDIAGKDGIREIKKVQRLIGIVPDESNLYDDLSGYENLVFCSA
                     LYGVRKSEREEKARSLLKQFGLDDTGKRPFKAFSKGMKRKLTIAAGLIHDPEILFLDE
                     PTTGIDVESARQIRSLVRELNDRGTTIFLTTHYIEEAERLCDRIGFIVGGRIVKIGTV
                     ADLMKEAQRENIVEFTLGSDPVDLKKQLIEEFSNVTINLRPGNTLRITSLEPIKLMPF
                     MSFFGSRGIDVYEAKIVRPSLEEVFVKVTGIEVEKMKTEKEGKKK"
     gene            complement(4246..4596)
                     /locus_tag="PKL93_09340"
     CDS             complement(4246..4596)
                     /locus_tag="PKL93_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ71499.1"
                     /translation="MNLEINVLKALADNTRYEIIKLLLRREFCVRALSRRLGISEPAV
                     SQHLKVLRSAHLVKGHKKGYFTHYSVDRGKLKELSNQLAELCEESSDTLLSDSIAILQ
                     ESISGSLDSSGESD"
     gene            4973..5215
                     /locus_tag="PKL93_09345"
     CDS             4973..5215
                     /locus_tag="PKL93_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71500.1"
                     /translation="MRKLINRILLAVALSPLVPAIVTLFPWEASKPNCVGYYSVCSFA
                     PYSSIILAGIAFATLGLVIATKRLLKRFLRLSDDLR"
     gene            5202..5639
                     /locus_tag="PKL93_09350"
     CDS             5202..5639
                     /locus_tag="PKL93_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNQ71501.1"
                     /translation="MIFDDKIGSLFEKIASLHINLSRHARLALEPHGITYPQFGALLV
                     LSSRGEMTQKQLSSHLETDTTNTMVICVGLEKKQLITREQSSEDKRANIVSITAKGRE
                     IFQKSMAVLRESYGPTANSISEKKRDTVNSVLDKLLESLNTLR"
     gene            complement(5658..6896)
                     /locus_tag="PKL93_09355"
     CDS             complement(5658..6896)
                     /locus_tag="PKL93_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HNQ71502.1"
                     /translation="MAEGAVDRRTRESINAFHVMVKPTGALCNLDCAYCFYLHKQELI
                     NSRNVISDEVLERFIYEYINSQTVPEIVFTWQGGEPTLLGIDFFKKVVDYERLYCPPG
                     KKIANDLQTNGTLLDEKWCSFLKEHNFLVGISIDGPERLHNVYRKSRSGGPTFQKVLN
                     AVRLLHKYEILFNTLTAVNRINAKYPGEVYRFLRDTAGSTRMQFIPIAEPKVFKSSPT
                     TFIPEDSMPITGSTEALPGKHASLVADWSVVPEDYGSFLNGVFDIWYREDIGKVFVFL
                     FECALAQWLGMEASLCIFSETCGKALALEADGSLYSCDHFVYPEHRLGNIKDRRLLEM
                     VTSTSQTVFGVSKAATLPEYCNKCEYLFACHGECPKNRFVKTPDGEPGLNYLCSGLKK
                     YFSHIDPYMKKLAEEYRSRK"
     gene            complement(6937..8547)
                     /locus_tag="PKL93_09360"
     CDS             complement(6937..8547)
                     /locus_tag="PKL93_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNQ71503.1"
                     /translation="MEIQSSRLLRKVLIGATGSALALTSALAANYEDTMPLETVKPAP
                     NLEEVLPHPEQREEAMAKLAEFESRTGKRPNILVYLMDDVGWGDLGVYGGGVAVGAPT
                     PNMDALARDGLMLTSAYSQPSSSPTRATLLTGQLPVRHGILRPTLYNEPGGLTNAVTL
                     AQLLSDAGYVTQAVGKWHMGENPGSQPQNVGFDDFSGFLSVSDLYTEWRDPYFYPEIA
                     NSEERTEMIREWAFEKYWVHAEKGGTIEQVKEIDIEVSSHLDEYWKDYSIEFIEKMAD
                     SDQPFFLYHCTRGAHFDNYPRDEYEGSSPAKQVYKDTIVELDDILGELVAALERTGQL
                     ENTLIFIASDNGPEMESWPDSAYSPFRGAKGSTWEGGVRVPAIFYWKGMIEPGRASDG
                     LFDIADIFNTCLTLAGATDSIPTDRFIDGVDQTSFLLAEEGLSNRKYIYYWLMSYFSA
                     VRVAEYKYVQFAESDSGEGIVNPGGFTGYIMAFPYYHLYNLYLDPKEEHNVSIRKLVF
                     TDLFLEAMKKHIDTFLEYPPVVEIITGH"
     gene            complement(9120..9467)
                     /locus_tag="PKL93_09365"
     CDS             complement(9120..9467)
                     /locus_tag="PKL93_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71504.1"
                     /translation="MSLAVIMVLLLGGCFSFDDMLDGTWSGVLNDYYGNHNVVLIVNS
                     NNTGWISFDNDPYNVDIVSRRANRSFVGEYGWYDSYWIERTIQAEMINYGTLRIKIYD
                     NFGDLISDGFLYK"
     gene            9649..10158
                     /locus_tag="PKL93_09370"
     CDS             9649..10158
                     /locus_tag="PKL93_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNQ71505.1"
                     /translation="METIVFKFSLPTSAIMLNIGIVVFVAVIFVFSLFKTKGWQKTIS
                     LIIIGSLFVFFIFLFLIFPFTNAVKFDGENVVLNALPFGRKTIAIDSAEIVGIIDWTE
                     ERDFEPTVRTNGASTGLYKVGWFRLRNGSKAFIMTARSEAFVMKSEDIYYLISPDELQ
                     EFADVIYSN"
BASE COUNT         2949 a         2543 c         2447 g         2881 t
ORIGIN      
        1 ttcatgacgc cgagaacggg gtatcgatta cagggtatgg gtcttgtgaa cagaactggc
       61 ttacaattca aggttttgct gcaactgagg aagccatccc gacatcggct ttcggaagtc
      121 ccttactctg tcatcccgaa cttgtttcgg gatctgttcc aagaacgaag aacccggaca
      181 tgtaccgtcg aagaacccgt attcttggcg gggacggtag accgttgacg ggcaacgtgc
      241 ttttcatgag cgtacagcgg ttcttaggat agagatgctg aaacaagttc agcatgacgc
      301 cgcgaacgag gtttcgatta cagggtatgg ggtctcgtga acagaatcgg ccatcagttt
      361 agggtttttc tgtaattgag aaagctaccc cgacatcggc ttttggaagt cccttactct
      421 gtcatcccga acttgtttcg agattaattc caagaacgaa gagcctggac gcgtagcgtc
      481 ggaacgagga accgcgattc tcggcgggga cggtggaccg ttgacggaca gcgtggtctt
      541 catcagcgta cagcgtgtac tctcaagcgt gaagtggctt ttctcggcga accagtttct
      601 cttgctcttc ctacttctga ccaccgacgt caaacctcgg taaacgatgg catgaaattg
      661 cttggtgagt gtggcatagc tttcaatcat atgaaataac cttgtgtaat catgtaataa
      721 attatgcaat cgaacaattt tcaagtcact ctctgtttgt ttgcaagcgc ttctaatttc
      781 tgtatcgttg aagaagagca aagaattttg cacggcagat gtgtgggagg caatgatgac
      841 cgagaagaga tcgatcaaag agattatgaa agcctattca gaagtccccg ataaggattt
      901 ccggcaaaag ctcaaaattt tgaatgagct ttcacagaca atgttcaaag agggcaaccc
      961 tcttctggcg tcggagactg ttaaagcgga gctttcaaga attgaagccc cggcatgtat
     1021 cgagctggca aacattacgc acaacatgat taagtactca tgcgttgtgg gcaattacga
     1081 ttcatcgatc gaatatgcct tgaaggcagt tgttcttttc aaggaattcg gaacggccga
     1141 cgatgtcaac gacgtgatcg gaaacattgg tggtgtgtat gtctacatgg ggcggtttga
     1201 agaatccctc gagtacaccg ataaggctct agaatacgtt cgaataaagg gtgatgatct
     1261 cgcggtagct aactttctga acaacaaggc cgtcgcattg aaaagtctgg aaagatttga
     1321 agaagccgtg gattgcttga tcaaggcaat agaaataaag ctctctctag gaaagaaagc
     1381 agatctctgc aacagctact tcaatctctc cgaagtgctg atcagcgcgg aaaggtatga
     1441 cgaagttccc gcgatcttca agaaaaccga acctctagtc aaagaaatcg aagatcctgc
     1501 agggtctgct gaattcgatt ttcttaaggc tcagtactac atgggaactg gcaactatgc
     1561 aaaggctctg gaaaaaatcg aaagttatat agagtatcag aaagagtctg gaagcatgtc
     1621 gaaagtgcct ggcgcactaa gaaataagat taagattcat gaagagcttg gagaagaggg
     1681 gctggctctc gaaacgtaca gggaacttga cctcttgtcc caaagaactt acaaggaaac
     1741 ctgcagtgcc agggccgctg agctggcggc ttcattcaga tttcagcaga agatgcacga
     1801 aatacagtta ctgagcaatc agaacttaga acttcaggaa gcaaagacaa ctatcgaaaa
     1861 gcaatatgaa gaacttctcg aaatcgacag caaactcaag aaggcaaatg agctactcga
     1921 gaaacaggcc gagatagatc cattgaccgg cctcctcaac cagaagaaga tgtaccccat
     1981 aatagaaaga gagataaata gagccacaag atacgggact cctctatcca tgatcatgat
     2041 agatcttgac aacttcaaaa acgtcaacga tacttatggt cacctgcagg gggacatcct
     2101 tctcaagaat gtggcgggca taatcaggtc ttctctaaga aaggttgact tcgttttcag
     2161 gtatggtggc gaggaatttc ttgttctttt gccctcaagc gatttgcaaa tggcaagttc
     2221 taccgcagat cgtctccggg atgagctggc caagaccaca tatcctaagg tgacgatgag
     2281 cgctggtgtc tccagctggt caggcgaaga tgcgactcat ttcattcaaa agaccgacca
     2341 cctgttatac gcagcgaaag gcaaaggaaa gaacagagtc gaatttcaga aaggctcttg
     2401 acagagtaat cggcgcagct tcaaccgaaa agaagacatt gtacttggtc ccaaaggatt
     2461 actgaaaaat cagactcatt taaccctgga ctctagtaga tccatcttct ctcaatattc
     2521 cgcgtactta ttaaaaagag cagtgcggca aagcccagca atataaaaac gctcgccaga
     2581 ggatctatga ttccatttct gtttatagcg tgtttaagag aatcggcacc ataggtcagt
     2641 ggaaggacga aggaaaatgg tctcaaaaac gccggcagat tctctatagg gatgaagaga
     2701 ccacaaagaa acatcatagg aaaacggaag aagttagaga acgtctgcgc ctcgaatact
     2761 tcgcttgccg aaaccgctat aaaaagacca agaaaggtcg aagtcacggc aatcaacaga
     2821 acactaactg aaaccaggaa ccagtttatt ccagataggt cgacaatcaa gagggcaaag
     2881 agaaccggaa caaaagcgtt gataattccg aaaagtattg ccccagtggt ttttgcaagc
     2941 attagaagct tgaggcttat gggagctaga agcaagcgtt caaatgacct actttttcgt
     3001 tcgaaagtta tcgtaactgc aagcatcgat gtagtcccaa agagaacgga gagagccata
     3061 acccccggca aaatgtctct tatttctacg tcgcttcccg atcgcacaaa gaacatgagg
     3121 gtccatgaga taggaaacac aattccccag cttatgtttg gtggcttcaa atagtagttc
     3181 ttcatatcct tcataaggat tgtccagtag gctactccac tcattttttc ttcccctctt
     3241 tctctgtctt catcttctca acctctattc cggtcacttt aacaaagacc tcctcaaggg
     3301 acggtctgac tatcttcgcc tcgtacacat caatcccacg ggaaccgaag aagctcataa
     3361 acggcatcag ttttattggc tccaaagagg ttattctaag tgtgttacca ggtcggaggt
     3421 ttatagtcac gttgctgaac tcttcaatca attgcttctt gagatcaacg ggatcacttc
     3481 caagagtgaa ttcaacaata ttttctctct gagcttcttt catcaggtcg gccacggtac
     3541 ctatctttac aattcttccc ccgacgatga aaccaatcct atcgcacaac ctttcggcct
     3601 cttctatgta atgcgtggtc agaaagatcg tggttccccg gtcgttcagt tctcttacca
     3661 aagaccttat ctgccttgcg ctttcgacgt cgatacctgt agtgggttca tcgaggaaga
     3721 gaatctctgg atcgtgaatt aagcccgcgg cgatcgtgag ctttcttttc atcccctttg
     3781 aaaaagcctt gaaaggtctc tttcctgtat catcaagacc aaactgcttc agcagagacc
     3841 tcgccttttc ttctctttca gactttctaa ccccatagag agccgaacag aagacaaggt
     3901 tctcatagcc gctaagatca tcgtaaaggt tgctctcatc aggaacaatc ccgatcaatc
     3961 tctgaacctt cttgatctct ctgataccgt ctttgcctgc aatatcgatc cttcccgaag
     4021 tgggtcttgc cagacccgtg agcatgttta tcgtagtagt ctttccggct ccgttaggtc
     4081 caaggaaacc gaatacctct cccttttcaa ccgagaagtc gatacttttc acggcagtga
     4141 agccgttgta gttttttgaa aggccggaga cttcaataat tcccaaagcg attacccccc
     4201 aaacttgtat atttcaccaa atccctatag acttatccta gaaacctaat cgctttcccc
     4261 actggagtcc aaactaccgc taatcgattc ctgcagaatt gcaatgctgt cagagagaag
     4321 agtatctgaa gattcttcac agagctcagc aagttgattc gacaactcct tcaactttcc
     4381 tctgtccaca gaataatggg tgaaatatcc tttcttatga cccttgacaa gatgcgcgct
     4441 ccgaagcacc ttaagatgct gagagacggc tggctcggaa ataccgagtc ttcttgaaag
     4501 agctctaacg cagaactccc tccgaagaag cagcttaata atttcgtacc ttgtattatc
     4561 tgcaagtgcc ttcaatacat tgatttcgag attcacaaaa atgttttctc caatccaatt
     4621 aagtgaatac ttaaccaaat tctagtccat tttttctact ctttcaacta agggtcgccc
     4681 acgttttctc atgtctgctt atttcctcaa gtgctttctt ctagcggtca tgtctatcta
     4741 ccgactggtg tctcacaaaa aactctttgt ttctcattca atactgatta tcgtaatcat
     4801 gattattatt aatatcggtg ttgaatgaag aaggtgaatc cgatatgaat gactgctcat
     4861 gcagagaaac gaagtgcgag ttccacggta tatgcgacag atgccgggaa aaacacgaaa
     4921 gactcgggtc gcttcctgcc tgtgaaagga agagaaggag gaatcggagg aaatgagaaa
     4981 actgatcaac cgcatcctgt tggctgtggc gctttctccg ctcgtaccag cgattgtcac
     5041 gcttttcccg tgggaagctt caaagccaaa ctgcgtcggc tactattctg tctgctcttt
     5101 tgcaccgtat agctccatta ttcttgcagg gattgcattt gctactctag gacttgttat
     5161 agcaaccaag aggctgctga agaggtttct gaggttgtcc gatgatcttc gatgacaaga
     5221 ttggaagcct attcgagaaa attgcttcgt tgcacataaa cctcagcaga cacgcacggc
     5281 ttgccctgga acctcatggt attacctacc cacagttcgg cgcactgctc gttctttcaa
     5341 gccgcggaga gatgacccag aagcagctct cctcacatct cgaaacggac acaacgaaca
     5401 ccatggtgat atgtgtcgga ctggagaaga agcagctcat aacgagagaa cagtcctctg
     5461 aagacaagcg ggcaaacatc gtctcaataa cagcaaaggg aagagagata tttcaaaagt
     5521 ccatggcagt gcttagagag agctatggtc cgactgctaa ttcaatttcg gagaagaaaa
     5581 gagacacggt gaactcagtc ctggacaagt tacttgaatc actgaacaca cttcggtaag
     5641 atgagttccg gactctctca ctttctcgac cgatactctt cggcaagctt tttcatgtat
     5701 ggatctatat gggagaagta ttttttcagt ccggagcaaa ggtagttcag tcccggttct
     5761 ccgtctggcg ttttgacaaa tctgttcttc gggcattctc cgtgacaggc gaaaaggtat
     5821 tcgcacttgt tgcagtattc cggcagtgtg gcagctttcg aaaccccgaa gacagtctgt
     5881 gatgtcgaag tcaccatttc gagcaacctc cggtccttaa tgtttccgag cctgtgctcg
     5941 ggatagacaa agtgatcgca ggagtacagg cttccatcgg cctccagcgc aagtgccttt
     6001 ccgcaagttt cagagaagat gcacaacgat gcctccatcc cgagccactg ggcaagggca
     6061 cactcaaaga ggaagacaaa aaccttgcca atatcctccc tgtaccagat atcgaaaacc
     6121 ccgttgagaa aactcccgta gtcttcagga acgaccgacc agtcggcaac gagcgaagcg
     6181 tgcttacctg gcagggcttc agtagatcct gtaatcggca tactgtcttc gggaatgaat
     6241 gtagtgggcg aacttttgaa taccttcggt tctgctatag ggatgaactg cattctggtt
     6301 gaaccggctg tatccctcaa aaagcgatac acctctccag gatacttcgc gtttattctg
     6361 ttaaccgcgg tcagggtatt gaaaagaatc tcgtacttgt gcaaaagtct tacagcattg
     6421 aggaccttct ggaaggtcgg accgccgctc ctgctttttc tgtagacgtt atgcagtctc
     6481 tccgggccgt cgatgcttat tccaacgaga aagttgtgtt ctttgagaaa cgagcaccac
     6541 ttttcatcca gcagcgttcc gtttgtctgg agatcgttgg caatcttctt gcccggagga
     6601 cagtaaagcc tctcgtaatc gacaaccttt ttgaagaagt cgattccaag tagtgtgggc
     6661 tcccctccct gccaggtgaa gactatctct ggaactgtct gtgagttgat gtattcatag
     6721 atgaacctct ccaggacctc gtcgcttata acgtttctgc tgtttataag ctcctgttta
     6781 tgcaaataaa agcagtaggc gcagtcgaga ttgcataggg ctcccgtggg tttgaccata
     6841 acgtgaaacg catttatact ttccctcgtt ctcctatcga ctgcgccctc tgccatttac
     6901 tgctctccct ctatgcgagc tatttcctta ttcgttctag tgacccgtaa tgatttcaac
     6961 tacaggtgga tattcaagga atgtgtcaat atgtttcttc atggcttcca agaagagatc
     7021 tgtgaaaacc agttttctta tggagacgtt gtgttcttcc ttcggatcca gatagagatt
     7081 gtacaggtga taatacggga aggccattat gtaaccggta aagcctccgg gattaacgat
     7141 tccctctccc gaatccgatt cggcaaactg tacgtatttg tactcggcta cccttacggc
     7201 agagaagtag ctcataagcc agtagtagat gtacttcctg tttgagagtc cctcctcggc
     7261 taggaggaat gaagtctggt ctactccgtc gataaacctg tctgtaggga tagaatcggt
     7321 tgcgcccgca agtgtaaggc atgtattgaa gatatcggca atgtcgaaga ggccgtcgga
     7381 ggcccgtcca ggttctatca ttcccttcca gtagaagatt gcgggtactc tgacgccacc
     7441 ttcccaggtt gaacctttcg cacctctaaa cggcgaataa gcgctgtccg gccatgattc
     7501 catctctggg ccgttgtcgg acgcgatgaa aatcagtgtg ttctcgagtt gtccagttct
     7561 ctcgagcgct gcgaccagct cacccagtat gtcgtcaagt tcgactattg tgtctttgta
     7621 aacctgcttg gccggagacg acccctcata ctcgtctctg ggatagttgt caaagtgagc
     7681 tcccctcgtg cagtggtaaa ggaagaacgg ctggtcgcta tcggccatct tttcaataaa
     7741 ttcaatcgag tagtctttcc agtactcgtc tagatgtgaa gatacctcga tatctatctc
     7801 tttaacctgt tcgattgtgc cgcccttttc tgcgtgaacc cagtacttct cgaaagccca
     7861 ctcccgtatc atctcggttc tctcttcgct gttggcaatt tcgggataga agtatggatc
     7921 ccgccactct gtgtacaggt ccgacacact gaggaaacct gagaagtcat caaatccaac
     7981 gttttgaggc tgagaaccag gattctctcc catgtgccat tttccaacag cttgagtaac
     8041 gtagccggca tcgcttaaca actgggcaag tgtaactgca tttgtcagtc ctccgggctc
     8101 gttgtacagc gttggcctta gtattccatg cctgactggg agctgtcccg taagaagagt
     8161 cgcccttgtt ggtgaagaag aaggttgtga ataagctgaa gtaagcatga gtccgtctct
     8221 tgcaagtgca tccatgtttg gagtcggcgc tcccacagct acacctccac cataaacacc
     8281 gaggtccccc caaccgacat catccattag gtatacaagt atgttgggcc gcttcccagt
     8341 tctgctctca aactcggcca acttcgccat tgcttcttct ctctgctccg gatgaggtaa
     8401 tacctcctcc agattcgggg ccggtttcac ggtttcgagc ggcatagtgt cttcatagtt
     8461 ggccgcaagc gcagatgtga gagcgagagc gcttccagtg gctccaataa gcacttttct
     8521 cagtaacctg gaactttgaa tttccataca accatctcct cttggattca ctttctaata
     8581 acctttctgg aagtgaaaaa ccacgaaata atttttgaaa gcagcattcc gggaccgttc
     8641 tcggataacc ttattggcat agaagaaaac gatcgaagat agttaatata gtcacaagat
     8701 aaatgccttc aagatactat cataaagacc ttcgaaatca gagcaaatgc cagatatcta
     8761 ctcgaagaaa acttcgcgct tcgaataatg aacaaatgca agccaaatct ctttcattga
     8821 ctactcctgg caggttccag atatctgcaa taactcgctt atgaagagat cttcggaaat
     8881 taacacgttt tgacatctaa aatttgcaaa gcaatagcgg aaagaaaaag tgataaggtc
     8941 gtttgagtta tattgattaa acttgcttgc cctgactaga accggtgaaa tataatgctt
     9001 ggattcggaa tctaccgtac aaaagaaaac tcccagaccg aagtttgaaa gttatagttt
     9061 caggaataat actagctttt ttgtatcagg gcggccgtac ttaagccgta agtacaaggc
     9121 tacttataca ggaagccatc gctgatgaga tcgccgaaat tatcgtatat ctttatccgc
     9181 aatgtgccgt agtttatcat ttcggcctga atggttctct cgatccaata ggagtcgtac
     9241 cagccgtact ctccgacaaa actccggttg gctcttctac ttactatgtc cacgttgtac
     9301 gggtcattgt cgaaagatat ccatccggta ttgttcgagt tcactatgag aacaacattg
     9361 tggttcccat aataatcatt cagaacacca ctccacgttc cgtctagcat gtcgtcaaat
     9421 gaaaaacatc cgccaagcag caataccata atcacggcta gagacaccag gtattgcttc
     9481 ttcatggagg tcgaacggcg ttacgataga agttttgcag tttgaaccct tcagataaag
     9541 agctctctgt ccgaaccgcc acaaagtcat gatgtcaacg atcatatgaa gatcaggagc
     9601 actcgaatgt gtaataattg aatcgaattt cctgtgagga ggcaagatat ggagacaatc
     9661 gtgttcaagt tttcattacc cacatctgcg attatgctga atattggaat cgttgttttt
     9721 gttgcggtga tattcgtttt ctctctattc aagacaaagg gctggcagaa gactatttca
     9781 ctaataatca taggcagtct gttcgtcttc ttcatatttc tcttcctgat atttccattc
     9841 acaaacgccg tgaaatttga cggcgaaaac gttgttctga atgcacttcc cttcggaaga
     9901 aagacgatag caatagattc agctgaaatc gttggaatta tcgactggac tgaagagagg
     9961 gacttcgagc cgacggtaag aaccaacgga gcatctacag gcctatacaa agtgggctgg
    10021 ttccgactca gaaatggctc caaggccttc atcatgactg caagatcgga agccttcgtc
    10081 atgaaatccg aggatattta ctacttgatc tctccggatg agcttcagga atttgccgat
    10141 gtaatttatt caaattagga atgctttatt gtaagaaacg tgttaggggg taacgggttt
    10201 gtggcatcaa gatcagaagt gaggccgact tcgtctggaa gtgatgctcg ctagcgcaag
    10261 aaaagacacc ctctcctctc tggtcgtaaa acgttgtgaa aaggcgtcct tcgagaagga
    10321 aagagccacc tggataagaa acactgaagg cttacgctgg aaaaatgctt cttcgacact
    10381 ttgtgtcaaa gttcttcgtt aaggaccacg agataaggag aaccgggcga gacgtttctc
    10441 tcgccttcta gtgagcatag cgaatgtctc gaaattatct tcctcgtcct atctcgatct
    10501 tgtctgagga caggtctatg atctgggacg aagggccaag gatggctctt gcttaagcat
    10561 gatgagatat gacgttctca gaattatcaa ctctcttata ttctccatgc gaagctctca
    10621 acaatcttct ttctcactaa ttctgatctt cgctcctaaa tcccctcttt agagctcttc
    10681 attacctata cgtcagggag tcaaatacca taaattagct gcttttttgc acgagtttat
    10741 atttggctcc taatgatcct gatcttaaat cctcccttga gggaggaggg ccacgtagtg
    10801 gcggagggtg ttggtctttg
//
LOCUS       DAOQYI010000049        10521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_6776_length_10521_cov_76.761131, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000049 DAOQYI010000000
VERSION     DAOQYI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6776_length_10521_cov_76.761131"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="PKL93_09375"
     CDS             complement(<1..1889)
                     /locus_tag="PKL93_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNQ71506.1"
                     /translation="MKRLGMILLFVLAVTSAFSNGVVIPQFPLEQDSGGFVMNTHNVH
                     ITVEDGIATVIIEEEFENTSTRLLEAVYLFPIPSSAVISDFTMKIGDQILKGEVLPAD
                     EAREIYQEIVAKMKDPALLEYVGTQLIRMSIYPFEPGERRQFFIEYSQPLEISSNSYS
                     MIYPLKIDSFLSAPIGSVSVRISVPLQIAEVYSPTHPFVETTGVSGREYVFIGNDLIP
                     NSDVALVLTSSEDEIPSSLATHWDESTNEGYFLLTLIPRIKEEIVIPKDVVFVLDISG
                     SMYGEKIEQAKRALEQVLQMLRPGDRFALVTFDGRVHNLTGSLLDASEKAEWIEKVRR
                     IQADGMTNIYDALQTSIDMFSKYDTGRFKALLFLTDGEPTEGITDIGRIISDATPEAR
                     ARNVHLFSFGVGTGVVAELLDRLVQENAGRVSYIVEGESIEAKVTDLYRSIETPALEN
                     VTVSIENLDVRKTLPEGPYSLFSGQALRISGIYFDEGDMRVTVEGTRGGERYRYEYLF
                     KTSKRSDSPFIERIWAQKRIAHLANLYRYDPSLTEAEKDELKLEIIALSKKFNIINEF
                     TSYLIAPERVQTYTAGDRGGLGAAPPADSVMAAKSVAEMEQDQFVEGGSSDYTFVDGI
                     GFTLE"
     gene            complement(2083..3045)
                     /locus_tag="PKL93_09380"
     CDS             complement(2083..3045)
                     /locus_tag="PKL93_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71507.1"
                     /translation="MKKAFVLTILVVFILSAFAMAAEKIKIGVAIPSADHGWTGGIVW
                     WAQRAIKDWSEKDPDIEFFLVTADSPAKQVGDVEDLMVKGIDALVILAHDSEPLTPVV
                     EKAYNSGVFIVSVDRGLTKPVENVYVAGDNPGLGRVSGEWLAEALNYKGDIVVLEGIP
                     CVINSERVDAFNEVMAKYPDIRILDSQPAYWDTARGLEIMENYLQKYPKIDAVWAQDD
                     DVLVGVLQAYKESGRNDIKLFLGGAGSKQMIKKIIDNDPLVKADVTYPPSMIATGISM
                     AVFALRGQPLEGFYQQQIPSKIILAAELITAENAELYYEPDSIF"
     gene            complement(3082..4056)
                     /locus_tag="PKL93_09385"
     CDS             complement(3082..4056)
                     /locus_tag="PKL93_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71508.1"
                     /translation="MAGENVKRRFEYERFGPLIALAILFVVSAIASPYFLQTQNLLNI
                     LRQVSYTGIIALGMTFVIISGGIDLSVGSMVALVGGVVILALNWLLTVFGEGGEAWAI
                     LFALIIGLLFGAGLGALNGLMVTIGKIAPFIATLGTMAIFRSMALYIGSAGVFRSESN
                     LFPDLGMGNVLGIPNPVVVFISLAVIFAIILNKTRYGRYLCAVGSNPKVAKYSAIKVN
                     LTRFISYVAVGITVGFSAVFLSARLNSMSSTNGGLNYELDAIAAVIIGGTPMTGGAGS
                     IFGTVIGAITLGIINNMLNMLGVSPYLQGTVKGVVIIGAVLIQRKRSQ"
     gene            complement(4057..5550)
                     /locus_tag="PKL93_09390"
     CDS             complement(4057..5550)
                     /locus_tag="PKL93_09390"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNQ71509.1"
                     /translation="MGEVLIEAKGISKEFSGVRVLDNIDIGIERGEIFGIIGENGAGK
                     STLVKILSGIYTPTEGKIFFEGKEVEIKDPLHAKMIGISIIPQEFNLINELSVYENIF
                     LGSEITRKGFLNTREMKRRTISLLEELKKSIDPEEKIERLSVAQKQMVEIAKAIAYDS
                     KLLIMDEPTTVLTLNEIEILFDLMRSLKERGVTIIYISHKLKEVRKICDRVMVLRDGQ
                     MISVSQTGEVDEEEMAKRMVGRELSQIFPDKVSPSDEVVFEVENLTINGLLENISFGL
                     REGEILGFAGLVGAGRTELAEAIIGIRKRDSGKISIKGKPVEIKSPNDAVKNKIAYLS
                     EDRQGSGILTSFDLIKNTTLISLPAYSKILINSKKEREKTKKYVDKFNIRAASLKTRL
                     EFFSGGNQQKVSLAKSLDPEPEIFIFDEPTRGIDVNAKREIYRFMNELVNEGVSCILI
                     SSELEEIIGMCNRVVVMKEGRITGILEGEHINEEEIMLHATGLKGVA"
     gene            complement(5562..6554)
                     /locus_tag="PKL93_09395"
     CDS             complement(5562..6554)
                     /locus_tag="PKL93_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNQ71510.1"
                     /translation="MGNRPVTLFTGQWADLTFEEICKKASSWGYDGLEIACWGDHMDL
                     EKAAKDPNYVEKRKEILEKYNLKCWALGAHLMGQCVGDLYDVRLDGFAPASCKGNPEK
                     IRAWGIEQMMYAPTAAKNMGCYTVTGFTGSPVWKFWYSFPQTTEKMVEDAYREIVDLW
                     TPIFEKFDAEGITFSLEVHPTEIAFDYYTAKRLLEVFNYRKTLGFNFDPSHLIWQGVK
                     PHLFVRDFAERIYHVHMKDAAVTLDGRAGILGSMMTFGDTRRGWNFRSLGHGHVNFEE
                     IIRELNNIGYSGPLSVEWEDSGMEREFGAKEAAEFVRKVDFEPSNIAFDDAMKS"
     gene            complement(6570..7733)
                     /locus_tag="PKL93_09400"
     CDS             complement(6570..7733)
                     /locus_tag="PKL93_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ71511.1"
                     /translation="MIEGKEVASAGQLRYGMVGGGEGSFIGDVHRKAIAIDGLAKLVA
                     GCFSIYYDDTVRTAKKLGVSDDRVYKDYKEMARKESEREDGIDFVSICTPNVTHFPIA
                     KEFLEANINVVCEKPLTIDESEARELADLAKKKNLLFAVAYAYSGYAMVKHAREMIQN
                     GAIGEIRVVMGEYAQDWLATRLEDTGNMQAAWRTDPSQTGKSNCVGDIGSHIENTVSY
                     ITGLKLKSLCASLDSFVPGRTLDDNAEILVRFNNGARGVFWASQVAVGHDNGLKVRIF
                     GSKGSIEWEQENPNYLKVAYVGEPVQILSRGGGYMLPNAASVSRIPAGHPEGYYEAFA
                     NIYRNFATTLIKKKSGEDISGADFPTVEDGLAGVRFIGKAVESSQKGSIWVDI"
     gene            complement(8050..9060)
                     /locus_tag="PKL93_09405"
     CDS             complement(8050..9060)
                     /locus_tag="PKL93_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71512.1"
                     /translation="MRMKRILSVFRIDTINGVRDKMVLYILVAPIIFSFFFRIIAPDF
                     QSLSLSFVSLKDQKATLEALEKFGSVEPVSSEAEMKERVGRSDDSVAIYKKDESFNLF
                     LQGNESEEIEEIAKIILTGLQSDTTRYIDFKESDQGRFVAPVTVFGFTFVVIISFVLG
                     GMVIGFNIIEEKESGAMRALMVTPIKKSELILGRSILGIIIPLIHSFLAVLILDLPTM
                     DYLKLIIVTISSSIIGIVVGFLIGVISSSQMSGIANMKVSGWLLLMPVILGFILPERL
                     HFIFFWSPTYWSFVVLKDIVNRSINWSSFAIQILWICLTTATLFLLMMSRIKKGLQTY
                     LN"
     gene            complement(9065..10111)
                     /locus_tag="PKL93_09410"
     CDS             complement(9065..10111)
                     /locus_tag="PKL93_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71513.1"
                     /translation="MRLFTNISKEIILGWRSHFFLLTVMLAVAYFLLMTFLIPEDLST
                     STQLILLTENEEIARAIEASDRSSESSLSFVNSRDELYESLNRSFNSVGVIMRGDTAS
                     PSFELVFQGHEDTKTRELVKLVVAHLLHGSDNQPISSNSVQYLQGKQDDGLDIPFNKS
                     FLPIFLMFEAVMMGMIMVFAMVFSEKSQKTLDAFAVTPGRVWEYLGAKVIFLMVLGIV
                     FAIILTVLTVGLQAKYLQLVLIVSVGSFLSTSVSLIAASFYENLSQSMAAILTLTVIF
                     SLPILSYYLEGFSPWYLRILPTFPILFSLQGAVFPHQGSTGMSYLILVAVEAVLAFVI
                     AVNLYKLRIRRVRM"
     gene            complement(10108..>10521)
                     /locus_tag="PKL93_09415"
     CDS             complement(10108..>10521)
                     /locus_tag="PKL93_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ71514.1"
                     /translation="PKVLFLDEPVSGLDPSTSAAIKELIKEKKKKGTTIFLTTHNMHI
                     ADELCDRVAFIVSGKISLIDSPRNLKLQHGKKLVEIEYRKNSSLKRESLSLVYEADRQ
                     KLNSLLDHEVIETIHSKEATLEEIIIKVTGEKLEI"
BASE COUNT         2796 a         2562 c         2121 g         3042 t
ORIGIN      
        1 tcttcaagag tgaagcctat tccatcaacg aatgtgtaat cagaagagcc accttcaacg
       61 aactggtcct gctccatctc cgcaacggat ttcgctgcca tgaccgaatc tgcaggaggt
      121 gcagcgccca ggcctcctct gtctcctgcc gtgtatgttt gaactctttc aggtgcgatc
      181 aggtacgaag taaactcgtt tatgatgttg aacttttttg agagagcgat aatttcaagc
      241 ttcaactcat ctttctctgc ttcagtgagg gaaggatcat atcggtaaag atttgcaaga
      301 tgagcaattc tcttctgagc ccagattctc tcgataaatg ggctgtcaga tctcttggaa
      361 gttttgaaga gatactcata tctgtacctt tcaccgcctc tggtaccttc aactgtcact
      421 ctcatatctc cctcatcaaa gtagattccc gatatcctca aagcttgccc tgaaaagaga
      481 gagtatggac cttcaggaag agtcttcctt acatcgagat tttctatgga aacagtcacg
      541 ttttccagag caggtgtctc tatggatctg taaagatcgg tgacttttgc ttcaatgctt
      601 tctccttcaa caatataact tactcttccg gcattttcct ggacaagtcg gtccagcagt
      661 tcggcaacaa ccccggtgcc tactccaaaa gagaagagat gaacatttct ggctcttgcc
      721 tcgggagttg catcgctaat aatccttcca atatcggtta ttccttctgt cggctctccg
      781 tcagtcagga acagaagtgc cttgaatcta ccagtatcat atttgctgaa catatctatg
      841 cttgtttgaa gtgcgtcgta gatatttgtc attccatctg cctgaatcct tctgaccttt
      901 tctatccact cagccttttc tgaagcatcg agaagacttc cagtaaggtt gtgcactctt
      961 ccatcgaagg taacaagtgc aaagcggtcc cccggtctta gcatctgaag tacttgttct
     1021 aaagcgcgct tagcctgttc aatcttctct ccgtacatcg aaccggaaat atcgagaacg
     1081 aagacgacat ctttggggat aacgatttcc tccttaattc tagggataag tgttaagaga
     1141 aaatatcctt catttgttga ttcatcccaa tgggttgcca gagaggaagg tatctcatct
     1201 tcggatgaag tgagaaccag agcgacgtct gaattgggaa tcagatcatt tcctatgaag
     1261 acgtactccc tgccgctgac tcctgttgtt tccacgaatg gatgggtcgg cgaataaacc
     1321 tcggcgatct gaagaggaac cgaaattcta acactcactg agcctatggg agccgaaaga
     1381 aaactgtcga tcttcagagg ataaatcatc gagtatgaat tagaagatat ttccagtggc
     1441 tggctgtatt caataaagaa ttgccttctt tcgcccggtt caaagggata gatactcatc
     1501 ctgataagct gagtcccaac gtactccagc aatgccgggt ccttcatttt cgccactatc
     1561 tcctggtata tctccctcgc ttcatctgct ggaagtactt cacccttcag aatctgatct
     1621 ccgatcttca ttgtgaaatc acttattaca gctgaagagg gaattgggaa cagataaaca
     1681 gcttccagca atctggttga tgtgttctca aattcctcct caattattac cgtcgcaatt
     1741 ccatcttcga ctgttatatg aacgttgtgg gtattcatca cgaatccccc tgaatcctgt
     1801 tccaaaggaa actgcgggat tactacaccg tttgaaaacg ctgaagtcac tgctagaaca
     1861 aaaagcaaaa tcattccaag tctcttcatt aaatcgcccc ctcttttgtc tagtacttat
     1921 gacatctcaa agagcctgaa ggtttatgca gtcgattttt tagaagagat atggggagga
     1981 gaaaaactcc ctttctaatt actctggata gttagtaagg cttttagttg aaaaaacccg
     2041 gagaccttgg tctccgggcg tctagattcg tgaggtgaaa atttagaata ttgaatcagg
     2101 ctcgtagtaa agctcggcgt tttcagcagt aatcagctct gcagcgagga ttatttttga
     2161 tgggatctgc tgctgataga atccttcaag gggctgacct ctcagtgcaa ataccgccat
     2221 ggaaattcct gtagctatca ttgatggagg atatgtaacg tctgccttca cgagaggatc
     2281 attgtcgatg atcttcttta tcatttgttt agatccggcg ccgccgagga atagcttgat
     2341 gtcatttctt ccagattcct tgtatgcctg aaggactcca acaagaacat catcgtcctg
     2401 tgcccaaaca gcgtcgattt tcggatactt ttgaaggtag ttctccatga tctctaatcc
     2461 acgtgcagta tcccagtaag ctggctgtga atccagaatt cttatgtccg gatacttcgc
     2521 cattacttcg ttgaatgcgt ctactctttc gctattaata acgcaaggga tgccttccag
     2581 aacaactatg tcgcctttgt aattgagagc ctcagccagc cactcgccag atactcttcc
     2641 aaggcccggg ttgtcaccgg ctacgtagac attctcaacg ggtttcgtca aacctctgtc
     2701 gactgacacg ataaaaacac cactgttata ggccttctcg acaacaggtg tgagtggttc
     2761 cgaatcgtga gcaagaataa ccagagcgtc gatgcccttt accatgaggt cttcaacgtc
     2821 tccaacctgt tttgcaggtg agtcggccgt caccaggaag aactcaatgt cgggatcctt
     2881 ctcactccag tcctttatgg ctctttgtgc ccaccatacg attccacctg tccaaccatg
     2941 gtccgcactt ggaatggcaa caccaatctt gatcttctcg gcagccatcg caaaagccga
     3001 gagaatgaac actacgagga ttgtaagaac aaatgccttt ttcacaagaa acacctcctg
     3061 tttcctttga cccccttggg gttattgaga tctctttctc tgtattagta ccgcaccgat
     3121 aattaccaca cctttgactg taccctgaag atatggcgat acccccagca tattaagcat
     3181 attgtttatt atgccgagtg tgatggcacc gataacagtt ccgaatatcg aacccgctcc
     3241 acctgtcatt ggcgttccac cgataatgac tgcggctatg gcatcaagtt catagttcaa
     3301 gccgccattc gtggaactca ttgaattcaa tctcgcagag agaaagacgg cggagaatcc
     3361 aacggtaata cctactgcaa cgtatgaaat gaaccttgtg aggttcactt tgatggcaga
     3421 atacttggca acttttggat tggagccgac ggcgcaaagg tatcttccat aacgagtctt
     3481 attgaggata atggcaaaaa tcactgcaag acttatgaat acaactacag gatttggaat
     3541 gcccaggaca ttacccattc ccagatcggg aaagagattg ctttcagatc taaaaacgcc
     3601 ggcgcttcct atatacagcg ccattgatct gaaaatggcc atagtaccga gtgttgcgat
     3661 gaagggagcg atctttccaa ttgttaccat cagtccgttc aacgcgccca atcctgcacc
     3721 aaaaagcaaa ccaattatca gcgcgaacag tatagcccat gcttctccac cctcgccaaa
     3781 tacggtcaga agccagttta gcgcaagaat cacaactcca ccaacaagag cgaccataga
     3841 tccaaccgaa aggtcgatcc ctcccgaaat aatcacaaaa gtcattccta gagctatgat
     3901 ccctgtatag gaaacctgtc tcaggatatt caaaaggttt tgagtctgaa gaaagtacgg
     3961 gctggcaata gcggatacta cgaaaagtat cgcaagggct attaacgggc cgaatctttc
     4021 atattcgaag cgtctcttta cattttcacc tgccattcaa gcaaccccct tgagtcccgt
     4081 ggcatgaagc atgatctctt cctcgttgat atgttcacct tcaaggattc ccgtaattct
     4141 tccctctttc atcacgacga ctctgttaca cattcctatt atctcttcaa gctcggagga
     4201 aatgagaata caagacacac cctcatttac cagttcgttc atgaatctgt atatttctct
     4261 cttagcgttc acatctattc ctctggtggg ttcgtcaaaa atgaaaatct cgggctccgg
     4321 gtcaagggat tttgcgaggg agactttctg ctggtttcca ccactgaaaa actccagtct
     4381 tgtcttgaga gatgctgctc tgatattgaa tttgtcgaca tacttcttcg tcttttcccg
     4441 ctctttctta ctgttaatga gtattttaga ataagccgga agcgaaataa gcgttgtatt
     4501 ctttatcaaa tcgaaggatg ttaatattcc actcccctga cgatcttccg aaaggtaagc
     4561 aatcttgttc ttcacagcat cgttgggtga cttgatttcc accggtttgc ctttgatact
     4621 gatcttgcct gaatctctct ttctgatacc aattatagct tcggctagtt cagttcttcc
     4681 cgcacccact agaccggcaa agccaagaat ttccccttca cgaagtccga aggaaatgtt
     4741 ttctagaaga ccattaattg tcaggttctc gacttcgaat acaacttcgt cagacggtga
     4801 aactttatct ggaaagatct ggctcagctc gcgtcctacc attctctttg ccatttcttc
     4861 ttcatcaacc tcaccagtct gacttacact gatcatttgg ccgtctctta gaaccatcac
     4921 tctatcacag atttttctga cttctttcag tttgtgagag atgtagatga tggtaacccc
     4981 tctttccttt agacttctca tgaggtcaaa gaggatttca atttcattga gagtaagaac
     5041 agtcgtcggt tcgtccatta tcaggagctt tgaatcgtaa gctatggcct tcgcaatttc
     5101 aaccatttgc ttctgggcca cgctcaacct ctcaatcttc tcttcgggat cgattgattt
     5161 cttcaactcc tccagaagag aaatggttcg cctcttcatc tctcttgtgt tgaggaaccc
     5221 cttgcgtgta atctcacttc ctaggaagat gttctcatag acactgagct cattgatcaa
     5281 gttgaattcc tgagggataa tagagattcc tatcattttc gcgtgcaaag ggtctttgat
     5341 ctccacttcc ttgccctcga agaagatctt tccttcggta ggtgtgtaga ttccgctgag
     5401 gatcttaacg agagtcgact ttccggctcc attttcacct atgatcccga aaatctcgcc
     5461 cctctctatt cctatgtcta tgttatcgag aaccctaacc cctgagaatt ccttagaaat
     5521 ccctttggcc tctatcagga cctcacccat tagttcactc ctcagctttt cattgcatcg
     5581 tcgaaagcga tgtttgaagg ctcgaaatca actttcctga caaactcggc cgcttccttg
     5641 gcaccgaatt ctctttccat tccactgtct tcccattcca ccgaaagagg accgctgtaa
     5701 ccgatgttat taagctctct gatgatttct tcaaaattga cgtggccgtg accgagagac
     5761 ctgaagttcc atccccgtct agtgtcacca aaggtcatca ttgatccaag tatccctgcc
     5821 cttccgtcca atgtaactgc tgcatccttc atgtgtacat gatagatcct ttctgcgaaa
     5881 tctcttacga agaggtgggg ttttactccc tgccagatga gatggcttgg atcgaaattg
     5941 aatccaagcg tctttctgta attgaatacc tctaacagcc tctttgcggt gtaataatca
     6001 aaggcgattt cggtgggatg aacttcaagt gagaatgtaa tgccttcagc atcgaacttc
     6061 tcaaaaatag gtgtccagag atcaacgatt tcccgatagg cgtcctcaac catcttctcc
     6121 gttgtttggg ggaacgagta ccagaacttc catacaggag agcctgtgaa gcctgttaca
     6181 gtataacagc ccatgttctt tgcagctgta ggagcgtaca tcatctgctc tattccccat
     6241 gctcgaatct tttcgggatt acctttacag ctggcaggag caaatccatc aagccttacg
     6301 tcataaagat ctcccacaca ctgccccata agatgggcac cgagggccca gcatttcaga
     6361 ttgtactttt cgagaatctc cttccttttt tcgacataat ttggatcttt cgcggccttt
     6421 tcgagatcca tgtgatctcc ccaacaggca atctcaaggc catcatatcc ccacgaactg
     6481 gctttcttgc agatttcctc aaaagtgagg tcggcccact gccccgtaaa caatgtgact
     6541 ggtctatttc ccatatcata acctcctgac tagatgtcta cccagatact ccccttctga
     6601 gagctttcaa ctgcctttcc gataaacctc actcctgcca agccgtcttc gactgttgga
     6661 aaatcggctc cagatatgtc ttctccactc ttcttcttta taagagttgt tgcgaaattc
     6721 cggtagatgt ttgcaaaggc ttcataatat ccttctggat gtccggccgg aattctagaa
     6781 acgcttgcag cattgggcag catgtatcca ccaccccttg agagaatctg gaccggttcc
     6841 ccaacgtatg ctaccttcag gtagtttgga ttttcctgtt cccactctat tgatcccttg
     6901 cttccgaata ttctgacttt aaggccattg tcatgaccaa cagcgacctg ggaagcccag
     6961 aagacacccc gtgccccatt gttgaatcta acgaggatct cggcgttatc atccagggtt
     7021 ctgcccggga cgaacgagtc aagactggca caaagcgact tgagcttgag gcccgtgatg
     7081 taggaaactg tattttcaat gtgacttcca atatcgccga cacagttcga ttttccggtc
     7141 tgtgagggat ccgttctcca tgctgcctgc atgttgccgg tatcttccag tcttgtagcc
     7201 agccagtcct gagcgtactc acccatgaca acccgaattt caccaattgc tccattctgt
     7261 atcatttccc ttgcgtgttt taccattgca taaccggaat aagcgtatgc aacggcaaaa
     7321 agcaaattct tcttttttgc caaatccgca agttcccgtg cctcagattc gtcgattgta
     7381 agaggctttt cacaaacgac gtttatattt gcttcaagaa attccttagc aattgggaag
     7441 tgagtgacat ttggagtaca gatacttacg aagtcaattc catcttctcg ctcagattct
     7501 tttctcgcca tttctttgta gtctttgtaa actctatcat cagaaactcc aagctttttg
     7561 gccgttctta cagtgtcgtc atagtaaatc gaaaaacatc cggctacaag ctttgcaagg
     7621 ccgtcgattg ctattgcctt tctatgaacg tcaccgataa acgagccctc gccaccgcct
     7681 accattccat atcttagctg accagcagag gcgacttcct tgccttcaat catctctctc
     7741 acctctcgta tatgatgttt tcttacgaac aaagcactaa gaagataagg aatttctcct
     7801 attctctaaa attatagcta aaaccagacc taattcttgc atcgcatctg accaaaagaa
     7861 cgagtgaaaa acgaaagaat tttgcgagaa actgcattga cttttgctgt tttcattaga
     7921 tgaagaataa tcatctcaga tcgttcctga agcgcctata ggatgttcaa gtgaattctg
     7981 gcagcatcga tttaattttt tgccccataa aaatatttat cagtttaaga caaaggcggg
     8041 ctacccgcct tagttgaggt aagtctggag tcccttcttg attctgctca tcatcaagag
     8101 gaataatgtg gcagtcgtta gacatatcca gagaatctga atggcgaaac ttgaccagtt
     8161 aatgctgcga tttacgatgt ctttgagtac gacaaatgac cagtatgtag gtgaccagaa
     8221 gaatatgaaa tgtagtcttt cgggaagtat gaagccaagt attacaggca tcaggagtaa
     8281 ccagccgctt accttcatgt ttgcaatccc gctcatctgt gaagacgaga ttactccgat
     8341 gagaaaaccg acgacaatac cgataattga gctagaaatg gtaactatta ttagtttcag
     8401 ataatccata gtgggcaagt cgagaatcag aacggccaga aatgagtgaa tcaaaggaat
     8461 gataatccca aggatgcttc ttccgagaat gagttcgctc ttttttattg gtgttaccat
     8521 gagagctctc attgctcctg attctttttc ttcgatgata ttaaacccta ttaccattcc
     8581 tcccaaaaca aaggagatga ttacaacgaa ggtgaatccg aatactgtta ccggtgcgac
     8641 aaatcttcct tgatcgcttt ctttaaaatc gatatatctc gtcgtatcag attgaagtcc
     8701 tgtgaggatg atctttgcaa tctcttctat ttcttccgat tcgttaccct gaagaaagag
     8761 attaaatgat tcgtctttct tgtatatagc cacagagtca tcagatcgtc cgactctttc
     8821 tttcatttcc gcttcggatg agacgggttc gacgctacca aacttctcaa gagcttccaa
     8881 agtggccttc tggtctttca gagaaacgaa cgataaagaa agactctgaa aatcaggagc
     8941 tatgattctg aaaaagaatg agaagattat cggggccacg agtatgtata gcaccatctt
     9001 atctctaaca ccgttgatgg tgtctattct gaaaacactg agtatccttt tcattctcat
     9061 gatctcacat ccgaaccctt cgtattctga gcttatagag attgactgct atcacaaaag
     9121 ccaacaccgc ttcgaccgct accaatatca ggtaggacat acctgttgaa ccctgatgag
     9181 gaaaaacagc tccttgcaac gagaagagaa tgggaaaagt aggaagtatt ctcagatacc
     9241 agggagagaa tccttccagg taatatgaaa gtatcggtaa tgaaaaaatt accgtcaacg
     9301 tcagaatagc ggccattgac tgagataggt tctcatagaa acttgcagct attagagaaa
     9361 cagatgtcga gaggaagctc ccaactgaga caatcaaaac aagctgcaag tacttagctt
     9421 ggaggccaac cgtaagaacc gtgagaatga tcgcgaaaac tatccctaaa accataagaa
     9481 agatcacttt ggctcccaga tattcccaga ctcttcccgg tgtcaccgca aatgcgtcga
     9541 gggtcttctg agatttttcg ctgaatacca ttgcaaagac cattatcata cccatcatta
     9601 cggcttcaaa catcaggaag atcggaagaa aagacttgtt gaaggggata tcgagtccat
     9661 catcttgttt tccctgaagg tactggacac tgttgctgga aatcggctga ttatctgaac
     9721 catgtagaag atgggcaacc actagtttca caagctctct ggttttcgtg tcttcatgtc
     9781 cctggaaaac cagctcaaat gatggggaag ctgtatcacc tctcataatt acgccaacac
     9841 tattgaaact tctattaagt gattcataga gctcatcacg actgttgaca aatgaaagtg
     9901 acgattcgga actacgatca ctggcttcga tcgctctcgc gatttcctcg ttttcagtta
     9961 gaagaattag ctgtgtgctg gttgaaagat cctcgggaat cagaaatgtc atcaaaagga
    10021 agtaggccac agccagcatt acggtaagca ggaagaagtg actcctccaa cctagtatta
    10081 tttccttaga gatgttcgtg aagagtctca tatttctaac ttctctccgg tcactttaat
    10141 gatgatctct tcaagggtag cttctttaga atgaatcgtc tcaatcacct catggtcaag
    10201 aagagaattg agcttctgcc gatcagcttc gtaaacaaga gaaaggcttt ccctcttcag
    10261 agaactattt ttcctgtatt caatttccac cagtttctta ccatgctgta gttttagatt
    10321 ccttggggag tcaatgagcg aaatctttcc agaaacaatg aatgccaccc tatcacatag
    10381 ttcatcggcg atgtgcatat tatgcgtcgt cagaaagatc gttgtgccct ttttcttctt
    10441 ctctttgatt aattctttta ttgctgcgga agttgatggg tctagcccgg aaacaggttc
    10501 gtccagaaac aagactttcg g
//
LOCUS       DAOQYI010000050        10382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_6886_length_10382_cov_60.877215, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000050 DAOQYI010000000
VERSION     DAOQYI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6886_length_10382_cov_60.877215"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="PKL93_09420"
     CDS             complement(<1..1535)
                     /locus_tag="PKL93_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ71515.1"
                     /translation="MKDLLREQLIRIVQEYGVDILKDTGKVKGLLKDFCPEEPRGKIF
                     AITQCLREGIHRELIDSSKRKTIRIELSRLIRKIHDNFGLKEDISRWAVETIALALGI
                     ISEKETVITTERSQVIEQKRVETHKVISHERIVEERGKRTTNGDELIYVERGSFIMGD
                     TWGDGFPYEKPTHKVTLTYDFHIGKYPVTFEEYDKYCMRTSAKRPGDFSWGRGRRPVI
                     NVSWWDAISYCNWLSEKEGIPKAYDDKGNILDKYGKTTTDITEVVGYRLPTEAEWEYA
                     ARGGNKSKGYKYSGSDNADDVAWCYQNSGDELHEVGTKLPNELGLHDMSGNTWEWCSD
                     WDDSYSSSPQTNPVDCRPGQYRVTRGGSWDDTSIDVRISCRDGGITADKHDVVGFRIC
                     RTNTPTNLGVEVDVSSLGLESPSSALSEAVEVMRDNSAGRDENKGPANVESQSEGNTA
                     QNPSIEVVESLDDKTLSANSMEGDPIGANQSLSIENISTSYDKSTKEKVLRTTNGDEL
                     IYV"
     gene            complement(1583..3010)
                     /locus_tag="PKL93_09425"
     CDS             complement(1583..3010)
                     /locus_tag="PKL93_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNQ71516.1"
                     /translation="MVQEVRDQLMRLVDSYGCNILEHPIRVRNLLNDLCKSSFKKEIH
                     AIFVVLKAGAYTKLVDSAKSSSVSTTIDSLIERIQDDYAIEDSLSSWSVECICESIGI
                     IYQKDSSVSNPPIIDGVALPNSTVEAFLEDEREITGIQDLEAKETEFEIVRKTSENPP
                     RNDESKIDFKQTPETSLSKRKKRKHLRTAVFIFIVAGLVIMAISVSNLAGNKTFQYSY
                     KASDLVPTMVFVKKGSFMMGDSMSAGSDNENPAHNVTFTYDYEIGMQEITFEDYDKFC
                     LDRGRKEPSDQYWGRENRPVIYVNWFDAIAYCNWLSEKEGLPKSYDLSGALIDKNGRS
                     TLDPSEVAGYRLPTEAEWEYAARGGNKSKSYVYSGSDSIDSVGWYDLNTEWRTEETGR
                     KLANELGIHDMSGNVAEWCTDSYAKYLSSSLTNPYVADGSKRVIRGGSWYNSADSCRV
                     TSRSNSSPETTFLSYVGFRICRTLF"
     gene            complement(3070..4305)
                     /locus_tag="PKL93_09430"
     CDS             complement(3070..4305)
                     /locus_tag="PKL93_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNQ71517.1"
                     /translation="MGKGVREALISIVKEKGFSVFDNPRELESLLVNLCNDHKNELSA
                     ILPAIREKFSDRLVEAAKTKSLSLAISDLIIILCKDYALSSEAAKWAVESLAVSLDLE
                     SEREPKNSISKDNSLRKVESEKKTTNGDYLILVMSGSFVMGNTLDNVDHVSANDEAKG
                     DLKRQKPPHKVNLTYDFYIGRFPVTFEEYDRFCEELGIEKPNDQGWGRGKRPVIDVTW
                     YEAIAYCNWLSDKEDIPRAYDNGGNLLNENGSVTNDPSLVVGYRLPSEAEWEYAARGG
                     KKSVGFRYSGGNDVDRVAWFIANSCRRYNPKKLERALLSGKTNEVGLKAANELGLNDM
                     SGNVWEWCSDWAAKYKAIPRINPYESKGTFKVTRGGCWGSTKAGVTVSKRGGDLPTNR
                     SQSLGFRICKTLILESFQS"
     gene            complement(4374..5210)
                     /locus_tag="PKL93_09435"
     CDS             complement(4374..5210)
                     /locus_tag="PKL93_09435"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNQ71518.1"
                     /translation="MHKKKGLPNQDAFASKISERGKHVMIAVADGHGDSKCFRSQVGS
                     RIAVKIAIDLLEEVSKCSSKSSNLSILKGNLESSFSIKLHSLWKKEVEKSFLTAGFSR
                     LNVTRPEISFDLRSEKPPCISEDSYLAYGTTLVSALVTRNLLMFAQIGDGDVLVISSN
                     GEIYSPVPDDERLLGNATTSLCSPEASKDFRFFIERLSGKPPLLIMLSTDGLSNSFAS
                     EEEFKEIARTLLDILNTQGMEYLKSNIGCFMNDLSSDRVGDDITIGIIFNEEVLHRKS
                     GH"
     gene            complement(5254..5961)
                     /locus_tag="PKL93_09440"
     CDS             complement(5254..5961)
                     /locus_tag="PKL93_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ71519.1"
                     /translation="MSFKREEQSARPLYLFLMCDSSGSMHGIKINSLNTAIREAIPEM
                     RRIAEENPFAGLFVRVVEFSQGARWLSDEAVPIEEFVWADLSAANQTDMGAAFKMVAE
                     ALKIPPMPKRAYPPVLVLVTDGQPTDEYETGLSEILNTVWGEKAVRIAIGIGEDCDFD
                     VLEEFMDGMPIAPLEARNAAALVNHIKWASTTVIKEVSQPSSKPKARELDRRKESDSV
                     WIPQPPAPGTDPDDFEF"
     gene            complement(6148..7479)
                     /locus_tag="PKL93_09445"
     CDS             complement(6148..7479)
                     /locus_tag="PKL93_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71520.1"
                     /translation="MGQLLKPGQKLLMNNSRIACTVEEFIGGGGQGEVYKVIGDQNYA
                     VKWYFPEYLREDTDLKRRIETLINYGAPDENFLWPLDLMISAEEQEETEKSFGYVMLL
                     RQQRFKSFKDFINVKVHPKFRTLASIGYELARSFLDLHAKGLSYKDINFGNVFFDPDT
                     GEIRICDNDNVDEDKAPGTIIGTPKFMAPEIVRGEAGPSSYTDLYSLAVLLFYLLMAN
                     HPLEGNLDSKIQCFDLEEQQKLYGEHPVFIYDPEDDSNRPDPERHKNVIRLWDFYPRF
                     IRKLFEQAFTTGLTNPESRIRESEWKNALIRLKDSIHYCSNCSEEIIHDFENSDETSV
                     CWFCKKPLASQMYLEIGSQKIIINHDTELFLHHVDKDQQFEFSEPIAKVVRHPNDPSR
                     SGFRNLSDSEWKVAASGKEFTIEKGRATVLVPDMEINFGKQTGTIKVLNKE"
     gene            complement(8411..9271)
                     /locus_tag="PKL93_09450"
     CDS             complement(8411..9271)
                     /locus_tag="PKL93_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71521.1"
                     /translation="MKKKGFAVLAVIIIVCVAGVGIGGAVWWFSRPSGAVELLSIKKT
                     LSDMGNKIEANEKAIKAAEQLVVELEKEQIKQVEKLAEASLKEREVEDDFKAALDDYN
                     LSAKRANRLQEELSKFVGESDLNKIVEIGGVEASLREHQMNLSHINTETSLKSQTADL
                     KRQYYEGTQQHVKNFRAAIDKLRFDIMEWNNKIVQFKIILQNNELQRTFIEDQADLYA
                     GSKSKLKSLIDTAESIENAFLQQQEVNSIISEIEGVQKNPYQWDDIQEPMPEEVQIPE
                     GGWEEMQYEF"
     gene            complement(9956..10141)
                     /locus_tag="PKL93_09455"
     CDS             complement(9956..10141)
                     /locus_tag="PKL93_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71522.1"
                     /translation="MTEKIIIEIHVELVFPENILRILEMVQEYELPQHSNVSLRIWIV
                     SVTESLDYIELFKETSD"
BASE COUNT         2868 a         2432 c         1784 g         3298 t
ORIGIN      
        1 tcgacataga ttagctcgtc tccgtttgtt gttcttaaca ccttttcttt agttgactta
       61 tcataagaag tcgatatgtt ttcaatcgaa aggctttggt tggctccaat gggatctcct
      121 tccatagaat ttgcagaaag ggttttgtca tcaagcgatt caactacttc aatagaagga
      181 ttttgagccg tattaccttc actttgtgat tcaacattcg cgggaccctt attttcgtct
      241 ctgcctgccg aattatctcg cataacctct accgcttcac ttaacgctga tgaggggctt
      301 tctaatccta atgatgacac atcaacttcg actccaagat tagtgggggt attcgtcctg
      361 catatgcgaa agccaacgac atcatgctta tccgcagtga ttcctccatc ccgacatgat
      421 atacgtacgt cgatggaagt atcatcccag cttcctccgc gagtaactct gtactgacca
      481 ggtctacaat caacggggtt tgtttgtggt gaacttgaat atgagtcatc ccaatccgaa
      541 caccattccc atgtattacc agacatatcg tggaggccaa gttcgttagg cagctttgtt
      601 cctacttcat gtaattcatc tccagagttc tgataacacc aggcaacatc gtctgcatta
      661 tcgctaccag agtatttata tcccttgctc ttgtttcctc cccttgcagc gtattcccat
      721 tcggcttcag tgggtaacct gtaaccaact acttcagtta tgtctgtagt tgtctttcca
      781 tatttgtcca ggatgttacc tttatcgtca tacgccttag gaattccttc cttttcactc
      841 aaccagttgc agtaagatat agcgtcccac cagcttacgt ttattaccgg tctccttccc
      901 cttccccaac taaagtcacc cggtcttttt gcacttgtac gcatacagta tttgtcatat
      961 tcttcaaaag tcactggata cttgcctatg tggaaatcat atgtgagcgt aaccttatgt
     1021 gttggcttct cgtatgggaa accgtctccc catgtgtctc ccattatgaa gcttcctctc
     1081 tcgacataga ttagctcgtc tccgtttgtt gttctctttc ctctttcctc aacaattcgt
     1141 tcatgagata ttaccttatg agtttctaca cgcttttgtt cgattacttg agatctctcg
     1201 gttgttatta cagtttcctt ttcgcttatt atccccagag ccagagcaat agtttcgact
     1261 gcccatctgg atatatcttc tttgagtcca aagttatcat gaatctttct tatgagtctg
     1321 ctcaactcta ttcttatcgt ctttctcttg cttgaatcta taagctctct atgtattcct
     1381 tctcttaggc actgtgtgat tgcaaatatc tttccccttg gttcttcagg gcagaagtct
     1441 ttcaggagac ccttcacctt tcctgtgtct tttagtatgt ctactccata ctcttggact
     1501 attctgatta gctgttccct taatagatct ttcatgttta tctccccgaa aacaggaata
     1561 tgacttcctt gattttgatg cactaaaata aggttctaca aatccgaaat cccacgtaac
     1621 tcaagaatgt tgtttctggt gaactatttg atctcgaagt aactctgcaa ctatccgccg
     1681 agttgtacca acttcctccc cttattacac gtttagatcc atcggcaacg taagggtttg
     1741 tcaacgatga actcaagtat tttgcataac tatcggtgca ccattctgcc acgttgcctg
     1801 acatatcatg tattcctaac tcgtttgcga gttttcttcc cgtttcttcg gtacgccatt
     1861 ctgtatttaa gtcataccag cctacagagt ctatactgtc tgaaccagag tagacataac
     1921 ttttactttt gttgccacct cttgcggcat actcccattc tgcttccgta ggaagcctgt
     1981 aacctgctac ttctgaaggg tctagcgtag atctaccgtt tttgtctata agagctccgg
     2041 atagatcata ggatttaggt aagccttctt tttcgcttaa ccagttacaa taagcaattg
     2101 catcaaacca atttacatat atgaccggcc tattttctct tccccagtat tgatcacttg
     2161 gttcttttct tcccctatct agacagaact tatcgtaatc ctcaaaagtg atttcctgca
     2221 tccctatctc gtagtcataa gtgaaggtca cattatgagc aggattctca ttgtcagatc
     2281 ctgctgacat cgagtcaccc atcatgaagc ttcccttttt gacaaatacc attgttggaa
     2341 ctaaatctga tgctttgtat gaatactgaa aagtcttgtt ccctgcgaga tttgacactg
     2401 aaatggccat aattaccaaa cctgcaacta taaaaatgaa cacggctgtt cgtaaatgtt
     2461 tccgcttttt tctcttggaa agagaagtct caggtgtttg cttaaaatct attttcgatt
     2521 catcatttct tggggggttc tcagaggttt ttcggacaat ttcaaactca gtttccttgg
     2581 cttctagatc ttgtattcca gttatctctc tttcatcttc taaaaaagct tctacggtcg
     2641 aatttggcag ggcaacacca tcaataatcg gagggtttga aacagaagag tctttctgat
     2701 aaatgatccc gattgattcg caaatacatt ctacgctcca cgaagacaag ctgtcctcaa
     2761 tagcgtagtc atcctggatt ctctcaatga gactatcaat cgtagtgctc accgaactac
     2821 ttttagcaga atctactaac ttagtatatg ccccagcttt taacacaaca aatattgcat
     2881 gaatctcttt cttaaaactg cttttgcaga gatcattcag aagattcctg actctaatgg
     2941 gatgctcaag aatgttacat ccatagctat caactaatct cataagctga tctctaactt
     3001 cctgtaccat ttgaaatctc ctatttgacg agatgacaga gaagtttagc cttcacgaga
     3061 tgcctattct cacgactgaa atgattctaa gatgagcgtt ttgcaaatcc taaatcctag
     3121 ggactgtgat ctattagtgg gtagatcacc accccgtttt gaaacagtta ctccagcctt
     3181 cgtacttccc cagcaacctc cacgagtaac cttgaacgta cccttgcttt cataaggatt
     3241 aatccgggga atggctttgt actttgctgc ccagtcggaa caccattccc aaacattccc
     3301 ggacatatcg ttaaggccga gttcattagc ggcttttaat ccaacctcat tagtttttcc
     3361 agataataag gctctttcaa gttttttggg attataccta cgacatgaat tggctatgaa
     3421 ccaagcaact ctatccacat catttcctcc agaataacga aatccaacgc ttttcttacc
     3481 accacgagct gcatactccc attctgcttc agagggcaat ctatagccca caaccaaaga
     3541 tgggtcgttg gtcacagaac cgttttcatt gagaaggtta cctccgttat cgtatgctct
     3601 cggaatgtct tctttatcac tcaaccaatt gcagtacgca attgcttcgt accaagtaac
     3661 atcaatcact ggtctctttc ctcttcccca gccttggtcg ttaggctttt caataccaag
     3721 ctcttcgcag aatctgtcgt attcttcgaa agtaaccggg aatcttccaa tatagaagtc
     3781 ataggtcaga ttgaccttat gaggaggttt ttgccttttc aaatctcctt ttgcttcatc
     3841 gttcgcacta acgtgatcta cgttgtccaa agtgttaccc ataacaaaac ttccactcat
     3901 aacaagaatg agataatctc catttgttgt ctttttttca ctttctactt ttctcagcga
     3961 attgtctttt gaaatactat tcttaggttc tctttcgctt tctaaatcta gtgaaactgc
     4021 aagcgattca actgcccatt ttgcagcctc tgaagaaagt gcgtaatcct tgcacaaaat
     4081 gataatcaga tcactaatag ctaaactcaa tgactttgtc ttggcagctt caaccaatct
     4141 atctgaaaac ttctctctta tagcaggaag aattgcagaa agttcattct tatggtcgtt
     4201 acatagatta acaagaagcg attctagttc tctaggatta tcgaaaacac tgaaaccctt
     4261 ctctttgaca atactaatta gagcttccct tactcctttg cccacgcctg ccaacccctt
     4321 tcgaagaaca acaactgtag taaacactaa agtttagaca aaataggtta ggtttagtga
     4381 cctgactttc tatgcagaac ttcttcattg aaaataattc caatagttat gtcatctcca
     4441 actctgtctg atgaaagatc gttcataaaa catccaatat tactcttaag gtattccata
     4501 ccctgtgtat ttagaatatc aagtaaggtt cttgcgattt ccttgaattc ttcctcgcta
     4561 gcaaatgaat ttgaaagacc atcggtcgaa agcattatta gcaatggcgg ctttcctgat
     4621 aacctttcaa tgaagaatct gaaatcttta ctagcttcag gtgaacataa tgaagtggtt
     4681 gcgttaccca aaagacgttc atcatccgga acaggggagt atatctctcc attgcttgat
     4741 ataacaagga catcaccgtc tccaatctgt gcaaacatca ggagattcct tgtcacaagc
     4801 gcagagacaa gagttgttcc gtaggccaga tatgagtctt ctgatataca aggaggtttc
     4861 tcgctgcgaa gatcaaaaga tatttctggt ctggtcacat tcaatctgga aaaacctgcc
     4921 gtaagaaagc tcttttcaac ttcctttttc caaagcgaat gcagttttat agaaaaactt
     4981 gattcaagat ttcctttcag aattgataga tttgaactct ttgaactaca ttttgatact
     5041 tcttcaagca aatcaatcgc aatcttcaca gcaattcttg aaccaacttg gctccgaaaa
     5101 catttcgagt cgccgtgacc gtctgccact gcgatcatga catgttttcc tctttcagaa
     5161 atcttagaag caaatgcatc ttggttcggc agcccctttt tcttgtgcat agctccgcga
     5221 accgactcac atatgacttt ccactttgtt tccttagaac tcgaagtcat ctggatcagt
     5281 acccggtgct ggaggctgtg gaatccacac tgaatcactt tcctttcttc tatctaactc
     5341 tcttgctttc ggtttgctgg aaggctggga cacttccttt atcactgtag tgcttgccca
     5401 ctttatgtga ttaacaagtg ccgcagcatt tctagcctct agaggtgcaa ttggcatccc
     5461 atccatgaat tcttccagta catcgaagtc gcagtcttca cctattccga tggcaattcg
     5521 tacagccttc tctccccaaa cggtattcag aatttcagaa agaccggttt catactcgtc
     5581 ggtgggctgg ccatcggtca caagaactaa aactggagga tatgcccttt ttggcatagg
     5641 tgggattttt aaggcctctg caaccatttt gaatgcagct cccatatctg tctgattagc
     5701 ggcgctaaga tctgcccaaa cgaattcctc tattggcacg gcttcatccg agagccagcg
     5761 tgccccttgg gaaaactcta cgactctaac aaacaaaccc gcgaaaggat tctcttcagc
     5821 tattcttcgc atttccggaa ttgcttctct tattgctgta ttcaatgagt ttatctttat
     5881 accatgcatt gaccctgaag aatcgcacat caagaacaga tacaatggtc ttgcgctctg
     5941 ttcttcgcgt ttgaaagaca ttccacacct ccagtaaata tcgttcagtt ttgcttatta
     6001 gtcgcgctat tggaaaaggt cttatatatg aattggcaat ttttgatttt gctatgtcaa
     6061 taatcttaga gatttagatc aatgaaaagt ctgtcatttg gtttttccaa agtcgcattt
     6121 gaagctcaag gtcatcatct cgatctacta ttctttgttt agcactttga tagtgcctgt
     6181 ttgttttcca aaattgattt ccatatccgg gacaagaacc gtagctctac ctttctctat
     6241 agtgaattct tttccagaag cagctacctt ccattccgaa tcactgagat ttctaaaacc
     6301 tgatctactt gggtcattag gatgtctcac aaccttggcg attggttcag agaactcaaa
     6361 ttgctggtct ttgtcaacgt gatgtaaaaa gagctcagta tcatgattaa tgatgatttt
     6421 ttggctccca atttccaaat acatctggct tgcaaggggc ttcttgcaga accaacaaac
     6481 agaggtttca tcagaattct caaagtcgtg aattatttcc tcgctgcagt tagagcaata
     6541 gtgtatactg tctttcagtc ttataagagc gttcttccac tcgctttctc ttattctgct
     6601 ctctggattc gttagaccag ttgtgaaagc ttgttcgaaa agtttcctaa taaaccttgg
     6661 ataaaaatcc catagtcgta taacattctt gtgcctttct ggatctggtc tgttggaatc
     6721 atcttccgga tcatatatga aaactggatg ctcaccgtaa agcttctgct gttcctccaa
     6781 atcaaagcat tgtattttgg aatccagatt gccctctagt ggatgattgg ccattaagag
     6841 atagaaaagt agtactgcga gcgaatacag gtccgtataa gaactcgggc cagcttctcc
     6901 tctaacaatc tctggtgcca tgaattttgg tgtaccaatt attgttccag gtgctttatc
     6961 ctcgtctacg ttgtcattgt cgcagattct tatttccccg gtatctggat caaaaaagac
     7021 attgccaaag ttaatgtctt tgtaggataa tcccttagca tgtaaatcga gaaagcttct
     7081 tgccaattcg tatcctatac tggcgagagt tctgaatttc ggatgaacct tcacatttat
     7141 gaagtcctta aaactcttaa acctctgttg tcttagaagc atcacatatc cgaaactctt
     7201 ctcagtttcc tcttgttcct cggcggagat catcagatcg agcggccata ggaaattctc
     7261 atctggagct ccataattta taagggtctc tatccttctt ttcaaatcag tatcctcgcg
     7321 gaggtattcc gggaaatacc attttactgc ataattctga tcccctatta ccttgtagac
     7381 ttcaccctga ccgcctccac ctatgaattc ttcaaccgta caagcaatcc gagaattgtt
     7441 cataagtagt ttctggcctg gcttcaaaag ttgtcccatg cagataacac ctccgaatag
     7501 tatttgatgc caagacacct ctacttccaa tcgctgttta atgatgtgtt taaaggttat
     7561 gcaacactca agaccatgta tgagatctat tagacattcc gaccagaaag aaacacataa
     7621 ttaaatactg aactgtcacg aacagaaaca tgaaccctac atagtcacat tcccaaacat
     7681 ctaatcaaat atgttataag ctctccatct agggagaaga atccatggaa ctaaataagc
     7741 tgaagcagtt ccaaaaattg agactgccta accacacgta caaaaacgtt atacattgca
     7801 tactaagctt tttaagggcg aagatcatca tcatgctgta cagataatct tgtctatctt
     7861 tatagatttg tgataaaccc aagacaaaag ctataaatac tgaaactcag actgtcaaac
     7921 agacttttca cctaatgaat gaacatcgct taataacata tcatggtaac ttaaaaatcc
     7981 aatagcaaga atcaaataga tcaccaccta tcaggcaatt tcatctgaaa tcgcaaatca
     8041 atttgcttta cgcaatttcg aaatactatt caacattctc aaattagccc ctgctatgaa
     8101 ccgccggtaa caaaactatt tctttcgaac ctcggttggt tctgatagga gcttgtagcc
     8161 acaattgtat aggacgatca aaacagatcg agggtgctct atctacatca gctgaaaacc
     8221 ccgaggaaca aaggaattcg aaatgttctg aactccttca actcctgcta caaagcttgc
     8281 caagccgaac tattacgaag gcaaaaaaat ctcctacctt cagcagcggc gacagcaaaa
     8341 ccgtataact atgcaaaaca agaacagcta taggaggaaa cgaaaatagg gagtagagtc
     8401 aagactaggt ctagaattca tattgcattt cctcccagcc accttccggg atctgaacct
     8461 cctcaggcat tggctcttgg atatcatccc actggtatgg gtttttctgc actccttcga
     8521 tttcagaaat aatactgtta acttcctgct gttgtaaaaa tgcgttctct attgattctg
     8581 ccgtatcaat taaactcttc aatttcgatt tgcttcccgc atacaaatca gcctgatcct
     8641 caataaatgt tctttgtagt tcgttgttct gtagtattat tttgaactga acgatcttgt
     8701 tattccattc catgatatcg aatctcaatt tgtctatagc tgctctaaaa ttcttgacat
     8761 gctgttgagt gccttcatag tactgtcttt tcaaatcggc agtctgggat ttcaaagatg
     8821 tttctgtatt gatgtgagaa aggttcatct gatgttctcg caagctagct tctacgccac
     8881 caatttcaac aatcttgttg agatctgatt ctcctacgaa tttactcagc tcttcctgaa
     8941 gtctatttgc ccttttagct gaaagattgt aatcgtccaa agcagctttg aaatcatcct
     9001 ctacttctct ttctttaagg ctcgcttctg cgagtttctc aacttgtttg atttgttctt
     9061 tttcaagttc aaccactaac tgctctgcgg ctttaatagc tttttcatta gcctcaattt
     9121 tgtttcccat gtcacttagc gttttcttaa ttgataagag ttccaccgcc ccgcttggtc
     9181 tgctgaacca ccaaacagca ccaccgattc caactccggc aacgcacacg ataataatca
     9241 ccgcaagtac tgcaaatccc ttctttttca tgcaacgcac ctcccttcaa tctaaaacgc
     9301 ttcacattct ttcactgaac ttaaaccctt gaggtaaatg tagattgctt cccgactaca
     9361 aaatcagaac caaaaaagtc cagaactacc taacattagt atgtctgttc tctcattcct
     9421 tggacaagta agtaatttcc taccgacaaa gtatccaaca cttttaggac tttgttcatc
     9481 gcaagtatcc aatcacaatc tagaaggtag ttctatcatc ttcaaaaact accgcaccat
     9541 ctcataaaaa ttcgcaacag ccgcttcaaa tgccaagatt gcgaagaagc gatcgtgcca
     9601 tgcaagagtt gccacataag ttgacctaag tgttctgcaa aatgggtgaa gcctaacaaa
     9661 ctgatcaact atctagtcat cgattatata tacaaataga ctcgaaacca gacggaaata
     9721 atacaaaatc gtagtagact cataccacaa atatatcact tgttacattc aattcaaaca
     9781 aaaacagttg gagcactttt tctacattat tgtctaactc attttgttca aaactgcaaa
     9841 gtgcttttct tcaagtcatc ttgttattat tgttttattg aaagaagtga ttcttttcca
     9901 ggaatcactt ccccaagtag tgagaacgga caatggaaca cattttgaag ccaattcagt
     9961 cagaagtctc tttgaaaagc tcaatataat ccagggattc ggttacagac acaatccaga
    10021 ttctcaagct cacatttgaa tgctgaggga gttcgtattc ctgaaccatt tcgaggattc
    10081 gcaagatgtt ctcaggaaat acaagttcta catggatctc tataataatc ttttcagtca
    10141 taaagagcgt aatctgatta tctgttgcca aacaagcgat aaataaaggg acttgccaca
    10201 gcttttcaag ggagcaaaag gtaactggta tcgccgcaag cactagaaga ctaataagtg
    10261 ccaatgattt gtttcacata acttcactcg aaaggtgaac atgatgaaag atctattaag
    10321 ggaacagcta atcagaatag tccaagagta tggagcagac atactaaaag acccaggaaa
    10381 gg
//
LOCUS       DAOQYI010000051        10167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_7064_length_10167_cov_69.607298, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000051 DAOQYI010000000
VERSION     DAOQYI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7064_length_10167_cov_69.607298"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="PKL93_09460"
     CDS             <1..1683
                     /locus_tag="PKL93_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNQ71523.1"
                     /translation="ADVLMEIDENGLLTVTETIDYVFKKPYRGIYRELPADRAGSQYS
                     AIEVTAVGKEIKYIESFGSQDARSVRIWFVPYNTGAVSPVKGEERVSVTYKYTVRRAV
                     EVGTDIAQIFRKIWGEDTASWVKKITATIVFPENLEILEFYTHPDAEVYREGNTYELE
                     VTNVPPLTYVEFRAVIPKDQAMAMNMRNVALGGAFDKNYIDGVESGVRRKILFGKWII
                     PIAIGLLTPFVFILAHRKLGREIDVGYYAEYERDLPTQHAPDVINAAVKNLAGMVDND
                     GIGSTIMELYRREYIDFEKGKGDKVEGIVIKNRDVSKLRPTEAAFLEFLDKYDTGNVF
                     DFSAVKKTVTKKQSEARIFTSDFSKYKSKVHDSVKGMHLIDLKGDAIAKGYSILMFVV
                     CVILLAFYSGADLAPLRIFSLIFFGAIWVLCWIVMVLPKDVFGRWTREGRLFYLKWLG
                     LKNYLEDYSLLNEKPPESIILWEEYLVYATALGIADTVRKNLNKIVPEDVWNEQSRHP
                     YMYYPVTAYAVSSFTGVTRAAYSASSKGSGGGGGFSGGGSGGGGGGGGTGGF"
     regulatory      1823..1989
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2068..3033
                     /locus_tag="PKL93_09465"
     CDS             2068..3033
                     /locus_tag="PKL93_09465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNQ71524.1"
                     /translation="MRIAIVGATGEVGRMMLKKLEEENIPAEVIDLYASSRSVGKKFT
                     LKDKTLEVVELREDSFDRGYDYVLFSAGASISRHYSPIAAEKGALVIDNSSAFRSDAE
                     VPLVVPEINGFLVKGYKGIIANPNCSTIQMVLSLSGIYRRYGVKTIVVSTYQAVSGAG
                     NKGVAELEKQEAGEIVSTVFIRQIHRNVVPVIGDLLETGFSTEEMKMVNETRKIFGDD
                     SISVWPTTVRVPVAHGHSESIFVETIQPFTPDGLKSEIENSQNVVYSEDHLTPLEVAG
                     SDLVYVSRLRTFDSSRFLFWNIADNIRVGAATNAVRIMKAHMGGV"
     gene            3033..3749
                     /locus_tag="PKL93_09470"
     CDS             3033..3749
                     /locus_tag="PKL93_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNQ71525.1"
                     /translation="MMRGAYYTATGNTFLVVDSRERKLSDMEKRLLVLKYVDGRDGAI
                     FVERDFMDYYNCDGNRAAFCGNGARTYVYFIHEKEKRNLVEFSSHAGNVLGKVDDLVS
                     VRMPSPRFLGTFEEEDYSGEIVVVGVPHLVIEGDTDKVDWNALLPLRHLYDANINVFT
                     VKKEGSLRIRTFERGVEGETGACGSGATSVVWLYSRRTGCEKVILQANGGDLTVSFRD
                     GSAYLGGGVEKCSEELELQL"
     gene            3719..>4227
                     /locus_tag="PKL93_09475"
     CDS             3719..>4227
                     /locus_tag="PKL93_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNQ71526.1"
                     /translation="MFRGTGTAVITPFKDGAVDFGSFRRFLRFQLENGIEALIVLGTT
                     GESPTINESERTKLIRIAVDEAGGKVPVIVGTGTNSTEKTIKSSLKAFDEGADGVLVV
                     VPYYNKPTQEGLYRHFGEIATRAQGPVIIYNVPGRTGANILPDTVIRCLEFNNIVGVK
                     EASGDQYQV"
     assembly_gap    4228..4327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4328..5002
                     /locus_tag="PKL93_09480"
     CDS             <4328..5002
                     /locus_tag="PKL93_09480"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ71527.1"
                     /translation="EGFAGEWCPVRYGLTGSTGRMGKEIIEVFKGHDLVLEVNDTGIF
                     RHGEPEVIVDFSNREVLKTTIELAAQFGAGVVIGTTALSQSDLTSLRTLSEKVPVIQS
                     YNFSTGINIMKMILREFSPLLKDFEAELTETHHSGKKDAPSGTALMLKDATERDLPIH
                     SLRVGGVPGDHSIVFANEGEVIEITHRAISRKVFAIGALTAARFCLEHEKGFYSFEEV
                     IIWTQR"
     gene            4987..5694
                     /gene="dapD"
                     /locus_tag="PKL93_09485"
     CDS             4987..5694
                     /gene="dapD"
                     /locus_tag="PKL93_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HNQ71528.1"
                     /translation="MDAEMIIRMIRDAKKKTPAVCYLSGDLRGLKHENVRFVGGKDFG
                     ILFGDLKEIEAILELHSERISAHYIEVKARNSALPLADLTKYHARIEPGAIIRDLVEI
                     GDNAVIMMGAVLNVGAVIGEATMIDMNVVIGGRAIIGANCHIGAGAVVAGVVEPPSAT
                     PVVIEDNVLVGANAVILEGVRVGDHSVIAAGAVVTKDIPPYSVAVGMPAKVVKRFDEK
                     TASKTSLVQELREIQES"
     gene            5695..6894
                     /locus_tag="PKL93_09490"
     CDS             5695..6894
                     /locus_tag="PKL93_09490"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNQ71529.1"
                     /translation="MIVQKYGGSSVSDTERIRNVARRIRKKVDAGEKVIVVVSAMGKT
                     TNELISLAESVTENPDPRELAMLLSTGEQVSAALLSMTLIEMGVRSISQNAMQLEIST
                     FGDFDNARIADLNLDKIYAELEKHDVIVVTGFQGVDPEGNLTTLGRGGSDTSAVAIAA
                     KAGVQCEILSDVPGIFTCDPKFHKDAKKLDYITYDEMLELAALGAKVLHSRSVEIAKK
                     YNVEILCLSSFSEEGGTRVVNRLPEWLEQPVVTGATIDTNQLKIAINKLPGNTDIVSK
                     IFQAVAGSSFNVDMISIVNDNGYIHLAFTVLSASPAAIKSTIERVLVDVDNWELAIDE
                     DVAKISTVGVGMRSSAGVAARFFLALQKAGAKIIATTTSEIKISVLVPKSQAGKALKA
                     LLDEFEL"
     gene            6904..8010
                     /locus_tag="PKL93_09495"
     CDS             6904..8010
                     /locus_tag="PKL93_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ71530.1"
                     /translation="MLSNVYSPFPLKIRSAHGIRIETDSGEYLDTFAGIGVLAFGHTD
                     RKTVDAVTSKVQRFSHTSNYFLDEDAISLSKFILDFSNSEGEVYFANSGTEATEAAIK
                     AVRRLKKGKLISFEGNFHGRTMGALSLTYSERLRKPFEPLLPDVRFIPKSVQDFEKAV
                     LSDEIAAVFVEVIQGNSGVHLYPPELLQAIEKLRKERGFLLVADEIQSGLCRTGEYFG
                     YQNYAIEPDIIILGKAVGGGLPLGAAVFRGVSPFYPGDHGSTFAPNPVSLAAGLSVLS
                     RMDSALLESVRICGAHLKENLERLPWAREVRGMGLMIGVSTENPERIKSLAFERKVLL
                     NVTAGSIRFLPALNITEEEIDEITERLDFGGLND"
     assembly_gap    8038..8137
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8138..8875
                     /locus_tag="PKL93_09500"
     CDS             <8138..8875
                     /locus_tag="PKL93_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNQ71531.1"
                     /translation="DVDARTHPHISTGLRAHKFGVPIEKVSEVADRIDGIHLHIGSQI
                     TDVEPFFDAYSKALDCAKQYGFKHIDLGGGWGIDYEGRELDIERLQKGISGIFAGFDG
                     KLIAELGRFIIGPAGYLVTKVVSVKPSEKIFIVTDAGMNALIRPVLYDAHHRIQVLAS
                     TKKTVTADVVGPLCESGDILARGRKLEVPIPGTAIVLENAGAYGFSMANNYNGMPFPA
                     EVLVDGEEVKLIRRRQSIEELFTNVKM"
     gene            complement(9031..10032)
                     /locus_tag="PKL93_09505"
     CDS             complement(9031..10032)
                     /locus_tag="PKL93_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ71532.1"
                     /translation="MKVTRRASLVVLLIVLLGGMLFGAGSEYTVVKGDTLWEIAKAYG
                     VDWKEIAELNGITDEFALQVGTVLKIPEAYTEISVMIPNIDHEIPAVICIPNGDGPFP
                     LVVMLHGTGSDKNEAGGGYLLAAPALAKAGIASVRFDFIGNGESTADYINYNFTSAVD
                     DTNIAFAYAASLPRVDGHRAGIMGWSQGGTIALLAAGQNPAYKSVLCWAGAPDLSGVG
                     SLEAYEIAKQNGYYELTFEWRSPLKLGLQWFDEAYGTDVLQVFSNSSAPVLAINGSED
                     TVVDPVNAQRIVDASRNEKSKVLIIEGADHTFNIFTGDMTAFNQLIEATVDWFSKTL"
BASE COUNT         2758 a         2204 c         2655 g         2350 t
ORIGIN      
        1 gcagatgtgc tcatggaaat agatgaaaat ggtcttctga cggtcacgga gacgatcgac
       61 tacgttttca agaaaccata tagaggcatt tatagagagc tgcctgccga cagggccgga
      121 agtcagtaca gcgcgattga agtaactgca gttggaaaag agatcaaata catcgagtcg
      181 tttggttctc aggacgctcg atctgtaagg atttggtttg tcccgtataa cacgggtgcg
      241 gtatctcctg tgaagggaga agaaagagta agcgttactt acaagtacac ggtaagaaga
      301 gctgtagaag tcggtacaga tatagctcag attttcagga agatatgggg cgaagatact
      361 gcaagttggg tcaaaaaaat cacagctaca atagtctttc ctgagaatct tgagatcctt
      421 gaattctata cacatcccga tgctgaagta tatagagaag gaaataccta tgaattggag
      481 gttacaaatg tgcctccact aacatatgtt gaatttagag cagtgattcc taaagatcaa
      541 gcgatggcca tgaatatgag aaatgttgct ttgggcggag cctttgacaa gaattacata
      601 gacggggtcg agagcggagt caggcgaaag atcctctttg gaaaatggat aatcccgatc
      661 gcgatcggtt tgctgacgcc ctttgttttc attcttgctc acaggaagct tggcagggaa
      721 atagacgttg gatattacgc ggaatatgaa cgagatctgc cgacacaaca cgcgcccgat
      781 gtcataaacg cagcggtcaa gaacctggcc ggtatggtag acaacgacgg gattggatct
      841 acaataatgg agctttatcg gagggagtac atcgatttcg agaagggcaa aggcgacaag
      901 gttgagggaa ttgttataaa gaaccgcgac gtttcaaagc ttaggcctac agaagctgca
      961 ttcctagagt ttctagacaa gtacgacaca ggaaacgtat tcgatttctc tgcagtcaag
     1021 aagacggtta ctaagaagca atcggaagca aggatcttca cgagcgattt ctcaaagtac
     1081 aagagcaaag tacacgactc ggtcaagggg atgcatctta ttgatctgaa gggagacgca
     1141 atcgccaaag gttactccat acttatgttc gtagtctgtg tgatcctcct cgctttctat
     1201 tcaggcgccg atcttgcgcc tttgagaatc ttctctctta tcttcttcgg tgcaatctgg
     1261 gttctctgct ggatagtgat ggtcctgccg aaggatgtat ttggtcgatg gacaagggag
     1321 ggaaggctct tctatctgaa gtggctcggg cttaagaact atctcgagga ctactctctg
     1381 ctcaatgaaa agccgcccga gtcgatcatc ctctgggagg agtatctcgt ctatgcaacc
     1441 gctcttggaa tagcggacac cgttagaaag aacctgaaca agattgttcc ggaagatgtc
     1501 tggaacgagc agagcagaca tccatacatg tactatcccg ttactgccta tgctgtaagc
     1561 tcttttacgg gtgttaccag agcagcctat tccgcctcga gcaaaggctc cggaggcggc
     1621 ggaggattca gtggtggagg ctccggcggt ggcggaggag gaggcggtac tggcggcttc
     1681 taggagtctc cgcctcttgc gactaggcta agcatatagc gatttgtgat gtcgcatcgg
     1741 acttaaatga gatgatcact taacgatatg ttttcattaa gggagttgag caccagacca
     1801 gcgtgatata atcaatcgaa agttacccaa cggaggagca cccgagatga gtagacgtcc
     1861 atcagggaat cgggacggat cgaaaggcga gggtgccgaa gaaggcggaa gccccagcga
     1921 ccgacttctg ggcatacggg aaatacctgt atgactgtca cgaagaatgc gtggagagcc
     1981 ggtgggttgg aataagagat tatttcaaac cattgaagtg ttcccgtttt cttcgggaac
     2041 actttttgtt attcaggagg tggtttagtg agaattgcaa tagtcggcgc aaccggggaa
     2101 gttggccgaa tgatgttaaa aaaactcgaa gaagagaaca ttccagcaga agtgatcgat
     2161 ctctacgctt ccagcagaag tgtgggaaag aagttcactc tcaaagacaa aactttagag
     2221 gtcgtcgagc ttcgcgaaga ttcttttgac aggggttacg attacgtgct cttctcggca
     2281 ggggcgtcga tatcgaggca ttactcgcca atagctgcag aaaaaggggc tctggtgatc
     2341 gacaattcgt cggccttcag aagcgatgct gaggttcctc tcgttgtgcc agaaatcaac
     2401 ggttttctcg ttaagggcta caaaggaatc attgcaaacc cgaactgctc tactatacaa
     2461 atggtccttt ctttaagtgg catatacagg agatacggag tcaagacgat agtcgtctct
     2521 acttatcagg cggtatcagg tgcagggaac aaaggagtcg ccgaactcga aaagcaggag
     2581 gccggcgaga ttgtttcaac cgtcttcatc agacagatac atagaaatgt cgtacctgta
     2641 ataggcgatc tacttgagac cggtttttca accgaagaga tgaagatggt caacgaaacg
     2701 agaaagattt tcggcgacga ttcaatttcc gtctggccta cgactgttcg agttcccgtt
     2761 gcacacggcc actcggaatc catattcgtc gaaacgatcc agccctttac gcctgacggc
     2821 ctgaaaagtg agatcgaaaa ctctcagaac gttgtttaca gcgaagatca tttaaccccc
     2881 ctcgaagttg ccggttccga tctcgtctat gtatcacgac tcaggacctt cgattccagc
     2941 agattccttt tctggaacat agcggacaac atccgcgttg gtgccgcaac aaacgcggtc
     3001 agaataatga aagcccatat gggaggcgtt taatgatgag aggagcatac tacaccgcaa
     3061 cgggaaacac ctttcttgta gtcgactcta gagagagaaa actgagcgat atggagaaac
     3121 gccttcttgt gttgaagtat gtagacggaa gagacggagc tatcttcgtc gaaagagatt
     3181 tcatggacta ctacaactgc gatggaaaca gagccgcttt ttgcgggaac ggggcgagga
     3241 cctacgttta tttcattcac gagaaggaga agcgcaattt ggtagaattc tcctctcacg
     3301 cgggcaacgt tttgggtaaa gtggacgatc tagtcagcgt acgaatgccg tctcccaggt
     3361 ttctcggtac ctttgaagag gaagattatt ctggagagat agtcgtcgta ggcgtcccgc
     3421 atctagttat cgaaggagac acagataaag tagattggaa cgctcttctg cctctgagac
     3481 atctttacga tgcaaatata aacgtattca ccgttaagaa agaaggaagt ctcaggatac
     3541 ggacattcga acggggtgtt gaaggcgaaa ccggtgcctg cggaagtggt gccacctcag
     3601 tagtatggct ctactcgcgg agaactggct gtgaaaaagt cattctgcag gcaaatggcg
     3661 gggaccttac cgtgtcattc agagatggat ccgcctatct tggaggaggt gtggagaaat
     3721 gttcagagga actggaactg cagttataac gccctttaaa gacggagccg ttgatttcgg
     3781 ctcattcagg cgattccttc gtttccagct tgagaacgga atagaggcat tgatagttct
     3841 cggcacgacc ggcgaatcac cgactataaa tgaatctgaa cgaaccaaac tgataagaat
     3901 tgcagtggac gaggcaggcg gcaaagtgcc cgtaatagtg ggtaccggga cgaattcgac
     3961 ggagaagaca ataaagagct cgctgaaggc ttttgacgaa ggtgccgacg gcgtgcttgt
     4021 ggttgtaccc tattacaaca aaccgacaca agagggcctt tacagacact ttggagagat
     4081 agctacgcgc gcacagggac cggttatcat ctacaacgta ccggggagga caggggcaaa
     4141 catcctgccg gacacggtaa tcagatgtct agagtttaat aacatcgttg gtgtaaagga
     4201 ggcgagtggg gaccagtatc aggtcgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnngaa ggctttgcag gagagtggtg tcctgtgaga tatggtttga ccggttccac
     4381 gggccgtatg ggcaaagaga ttatcgaggt ctttaaagga catgatctcg tgctcgaagt
     4441 taatgacaca gggatcttcc ggcacggcga accggaagtg atcgtggatt tttcgaatcg
     4501 cgaagtactt aagactacga tcgaactggc cgcgcagttc ggggcaggtg tggtgatcgg
     4561 taccaccgct cttagtcagt cagacctcac aagtctcaga acgctttctg agaaggttcc
     4621 tgtaattcag agctataatt tctccacagg cataaatatc atgaagatga ttctcagaga
     4681 gttctctcca ctactgaaag actttgaggc agagttgact gaaacccacc acagcgggaa
     4741 gaaggacgcg ccttcgggaa cagccttgat gctgaaagac gctacggaga gggatcttcc
     4801 cattcattcg ctgagggtgg gtggagttcc gggggatcat tccatagtat ttgcgaacga
     4861 aggcgaggta attgagataa ctcacagagc gatctcgaga aaagttttcg ccattggcgc
     4921 actgaccgcg gctcgattct gcctggagca tgagaagggt ttctactcct ttgaggaggt
     4981 aataatatgg acgcagagat gatcataagg atgattagag acgcaaaaaa gaagacccct
     5041 gcggtctgct acttatccgg tgatttgaga ggtctgaaac atgagaatgt ccgatttgtc
     5101 ggcggaaagg acttcgggat tctcttcggc gatctgaaag agatagaagc tatcctcgag
     5161 ttacattcag agagaatttc ggctcattac atcgaagtaa aagctcggaa ctccgctctt
     5221 cccctggccg atctgaccaa ataccacgca aggatagaac cgggagcgat tatacgcgat
     5281 ctcgtagaga ttggcgacaa tgcggtgatt atgatgggcg ccgttctgaa tgtcggagct
     5341 gtaatcggtg aggccaccat gatagatatg aacgtcgtaa tcgggggaag ggcgattatc
     5401 ggagcaaact gccatatagg ggctggggcc gttgttgcag gcgtggtcga accgcccagt
     5461 gccactcccg tcgttattga agacaacgtg cttgtcggag ccaacgcggt tattcttgaa
     5521 ggtgtaagag tcggcgatca ttccgtaata gctgccggtg ctgtggtcac aaaggatatt
     5581 ccgccctatt ccgttgcggt gggaatgcca gcaaaagtag ttaagcggtt cgacgagaag
     5641 acggcaagta aaacgagtct cgttcaagag ctgcgtgaga tacaggagag ctagatgatc
     5701 gttcaaaaat acggaggttc atctgtttcc gataccgaaa gaataagaaa cgttgcaaga
     5761 agaataagga agaaggtcga cgcaggggaa aaagtgatcg ttgtcgtctc tgcgatggga
     5821 aagactacga atgagctaat atctctagca gaatccgtaa ctgaaaatcc cgatcccagg
     5881 gaactggcga tgcttctttc caccggggaa caggtttcgg ccgctctact ctcgatgact
     5941 ctaatagaga tgggagttag atctatctcg caaaacgcca tgcagctgga gatctcaacg
     6001 tttggcgatt tcgataacgc gcgaatcgcc gatcttaatc tcgacaagat atacgccgag
     6061 cttgagaagc acgatgtgat agttgtcact ggatttcagg gtgtcgaccc ggaagggaat
     6121 ctaacaacgc taggccgagg aggttccgac acttcggcag tcgcaatcgc tgccaaagcc
     6181 ggtgttcaat gtgaaatact cagcgatgtc ccaggtattt tcacctgtga tccgaagttt
     6241 cacaaggatg cgaaaaaact ggactacata acatatgacg aaatgcttga actggcagcc
     6301 cttggggcaa aagtcctcca ttcaaggtct gtggaaatag caaagaagta taacgttgaa
     6361 atcctgtgtt tgtcttcatt cagcgaagaa ggaggtacaa gagtggtgaa caggctaccg
     6421 gaatggcttg aacagcctgt agtaactgga gcgactattg acacaaacca gttgaagatc
     6481 gcaataaaca aacttcccgg aaatacggac atcgtaagca agatcttcca ggcggttgct
     6541 ggatcgagtt tcaacgtaga catgatctcg atagtaaacg ataatgggta cattcatctg
     6601 gccttcactg tgctttcagc aagtcccgct gcaataaaga gcacgataga aagggtcctg
     6661 gtcgatgtag acaactggga gcttgctata gatgaggatg ttgcgaagat ttccaccgtg
     6721 ggagtaggca tgaggtcgag tgcaggagtc gctgctcgtt tcttcttggc ccttcagaag
     6781 gccggcgcga aaataatagc aacaacaacc tccgaaataa agatctctgt gttggttcca
     6841 aaatctcagg caggcaaggc cttgaaggcc ctgctcgacg aattcgaact gtgagatgat
     6901 gagatgctgt caaacgttta ttcgccattt ccattgaaga tccgcagtgc ccacggtatc
     6961 agaatcgaga cggatagtgg cgagtatctc gatacctttg ccggaatagg ggtccttgct
     7021 ttcgggcaca cggatagaaa gaccgtcgat gccgttactt caaaggttca gcggttctcc
     7081 cacacttcga attacttcct cgacgaagac gcgatttccc tcagcaaatt tatcctcgac
     7141 ttctcaaatt ccgaaggcga agtttacttc gctaattccg ggacggaagc cacagaggcc
     7201 gctataaaag ctgtaagaag gctaaaaaag ggcaagctga tttcgttcga ggggaacttc
     7261 cacggaagaa cgatgggcgc tctgtcccta acatacagcg agcggcttcg caaacccttt
     7321 gaaccgctac ttcccgacgt tcggttcatt cctaagagtg ttcaggattt tgaaaaggca
     7381 gtattgtctg atgaaattgc cgcggtcttc gtggaggtca ttcaggggaa cagcggtgtt
     7441 cacttatacc ctcccgagct tcttcaagcc atagagaagc taagaaaaga gcgaggtttt
     7501 ttgctcgtag cagacgaaat ccagagtggc ctctgcagaa cgggagagta tttcggttat
     7561 cagaactacg cgatcgagcc ggacataata atactgggaa aggctgtggg aggtggacta
     7621 cccttggggg ccgccgtgtt cagaggcgta tcgccctttt atcccgggga tcacggatcc
     7681 acatttgcac cgaatccagt cagcctcgca gcagggctct ccgttttgag tagaatggac
     7741 tcggctcttc tagaaagtgt tagaatatgt ggggcccatc tgaaggagaa tctggagaga
     7801 cttccctggg caagagaagt gagaggcatg ggactcatga ttggcgtttc aacggagaac
     7861 cctgaaagaa taaagagtct tgcctttgaa aggaaagttc ttctcaatgt cacagctgga
     7921 agcataaggt ttcttccggc gctgaatatt acggaagaag agatagacga gataactgag
     7981 cgactggatt ttggaggact aaatgattga ttttcagaaa cttgcaaatg agtttggnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat gtagatgccc gtactcaccc
     8161 gcatatatcg acaggcttga gggcccataa attcggcgtg cccatagaaa aggtctcgga
     8221 agttgccgac aggatagatg gaatacatct tcacataggc tctcagatta cggacgtgga
     8281 acccttcttc gatgcctatt ctaaggccct cgattgtgca aagcaatacg gattcaagca
     8341 catcgatctt ggtggaggct ggggaataga ctacgaagga agagagcttg acattgaaag
     8401 acttcaaaaa ggaatatccg gaatctttgc aggcttcgac ggtaaactga tagccgaact
     8461 ggggagattc atcataggtc cggccggcta tctggtcacc aaagtggtca gcgtcaagcc
     8521 ttcggaaaag atatttattg tcaccgacgc tggaatgaac gccttgataa gaccagtgct
     8581 gtatgatgcc catcacagaa ttcaggtcct cgcaagcaca aaaaagaccg tcactgccga
     8641 tgtggtcggt ccgctgtgtg aaagcggtga cattcttgcc agaggccgaa agctcgaagt
     8701 accgattccc ggaaccgcaa tagtcttgga gaatgccggc gcgtatggat tctcaatggc
     8761 aaacaactac aacggaatgc ccttccctgc ggaagtgctc gtggacggag aagaggtcaa
     8821 gctcattcgc cgcagacaga gcattgagga actatttact aacgtaaaga tgtgaagatc
     8881 gagatccgcc ctttcgataa atcgctgaat caaacgaaag ctcgcgactc attgggtaga
     8941 gactcagata aagaaaagca aaaagaggga ctgactcatt tcttttgtcc ctcttttttg
     9001 gtgacttata gcgctatcga agaacgattt ttacagtgtc ttgctaaacc aatctacagt
     9061 ggcttctatt agctgattga atgcggtcat atctccggtg aatatattga aggtgtggtc
     9121 tgcgccctcg attataagaa ccttcgattt ctcatttcta gatgcatcga cgattctctg
     9181 tgcattcacg ggatcgacta ctgtgtcttc tgaaccattg atagcaagca ccggtgcact
     9241 tgagttagag aagacctgca agacgtccgt tccgtaggcc tcgtcgaacc actgaagtcc
     9301 cagtttaaga gggcttctcc actcaaaggt cagctcatag taaccattct gcttcgcaat
     9361 ttcgtaagct tctaagcttc caacgcctga caggtcagga gcgccagccc agcagagaac
     9421 agacttgtaa gcgggattct gtcctgccgc aagcaaagcg atcgttccac cctggctcca
     9481 gcccattatc ccggcccgat gaccgtccac tcttggaaga cttgccgcgt aagcgaacgc
     9541 gatgttcgtg tcgtctacgg ctgaagtgaa attgtagtta atgtagtcgg cagtactctc
     9601 gccgttccca atgaagtcaa agcgaacact cgcaataccg gcctttgcaa gcgctggagc
     9661 tgccagcagg taaccaccac cggcctcgtt cttatcagag cctgttccat gaagcataac
     9721 aactaatgga aatggaccgt caccgttggg tatacagata acagcgggaa tctcatgatc
     9781 gatattggga atcatgacgg atatttccgt ataggcttca ggaatcttca agaccgttcc
     9841 cacctgaagt gcaaactcat cagtgattcc gttaagttcg gcaatttcct tccagtcgac
     9901 accatacgcc tttgcgatct cccatagagt atctcccttt acaacagtgt actcgcttcc
     9961 ggctccgaaa agcataccgc caagaagcac aatcaaaaga acaactagag aagctctcct
    10021 cgtaactttc aacgctctca cccctcaaaa aagatatggt ttcattttta gtatacaccc
    10081 gctttgttgc cggactgata ataagctcct cttgaaaacg ctcctgcaag aagagagaga
    10141 aagagaggga gagaagagag agagaga
//
LOCUS       DAOQYI010000052         9844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_7369_length_9844_cov_62.685770, whole genome shotgun sequence.
ACCESSION   DAOQYI010000052 DAOQYI010000000
VERSION     DAOQYI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7369_length_9844_cov_62.685770"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PKL93_09510"
     CDS             complement(<1..775)
                     /locus_tag="PKL93_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ71533.1"
                     /translation="MNNMVERMKSNIKKNYLFSFLMNMRLSSGLWMIYMAFKGMSLTQ
                     IGFLEGIFHVTSLMMEVPTGSVADLFGRKLSRTIGRCLSLASILVLIGATSFFHFTIF
                     MVLAALSYNLESGSGEALVYDSLKYLKTEENFISVLGRQEAIFQVSSVAALLLGGFLG
                     TYNYHYAFWVSAGIIAFSAVYSLSFTEPPILESVGRNSKTFRGFLKQIADSFSIIARD
                     RKVAFLIFFVQTILAFSACIFFYIQNYWKGLGRTEFQIGI"
     gene            complement(1081..2799)
                     /locus_tag="PKL93_09515"
     CDS             complement(1081..2799)
                     /locus_tag="PKL93_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNQ71534.1"
                     /translation="MLRIYNLSISFGSVDVLKKISFDLAAGEVLAVTGANGAGKSTLL
                     KSIIGEITPSDGKITIEQGIKPLLVNQETSDFHGTVKEYLLGAREELFDIYEKLGGSV
                     NDPMAYAELINEFHNVGGFEFETKISSSLNEFGIDVDNLDFPYMNLSHGQKRILSLIR
                     GVLSGSNLFLLDEPTNHLDIEMTLRLEEIITSLSERGVGVIVVSHDRRFIDKVAHKTI
                     CLKRGEAVGVRGGYSEMLAHLERDFLSRQKTSEEISKKIRQLELDATRRKSWSNSREA
                     SKRFASDKGHEGHMAAKLAKRALAVQKRTERLIGELECEKPFVEKPVNVRIPKYGVPK
                     RKMVMVESLSFSYGENEVIREASVNIDTSDRVGLIGPNGSGKTTLIKCLVGILELSSG
                     KIYRNESVNWMYIPQNVAELFERKTPYEEIADLELEEPAVRSHLANIGLKRERAFSEI
                     KTMSYGELMRLALLKSLLSKVEFIFMDEPTNHLDIESLEVLDKLLNDFSGGFFFISHD
                     RQFIAEHGEKILTIEEGMLKSFEYSEEIDIDSFTRTKEILADSYDESIFRRSSKNLLH
                     KSEEED"
     gene            complement(3008..3409)
                     /locus_tag="PKL93_09520"
     CDS             complement(3008..3409)
                     /locus_tag="PKL93_09520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ71535.1"
                     /translation="MAKENYSFTVEPDLYKIDAFSAKVSEIVKRTKGNETGFRAGLIV
                     IEACSNIVKHGELKEDDLISVNLEVGEEAVTITIEDASTKFNPLEVDDPNLENIELLQ
                     KGGMGVYLIKRFSKRVDYRYENGKNLLKITI"
     gene            complement(3414..3758)
                     /locus_tag="PKL93_09525"
     CDS             complement(3414..3758)
                     /locus_tag="PKL93_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNQ71536.1"
                     /translation="MFEFKKANDGIGVISLDSRARITGEMSANFRKWQETVDLKSCST
                     VIVDGNNIEFIDSMGIAALISIYKTVSAKGSDLILVNLSEEIKKLLNTLRLDRLFIVK
                     DCSVAEAIKDLC"
     gene            complement(3807..4928)
                     /locus_tag="PKL93_09530"
     CDS             complement(3807..4928)
                     /locus_tag="PKL93_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNQ71537.1"
                     /translation="MSRILVVDDDLSVRVLLKSILSRDNHDVVESADGRSAFSSLAVQ
                     KPDIILLDLMLPDYNGLDILAQLKESEELEPIPVIVLTGSADRESKLTALSSGAVDFI
                     SKPFLPEEVLLRVNTHLKLHDLIRSLRVAVDNLESDVIAAGKIQNALVPKSNPQGLSV
                     EWIYEPSYRVGGDIFDVFRLDENRYFVYLADMSGHGVNAAMLSVMVHRFIEDFRTSID
                     DGEFDLSSFMKELDRNFIFERFNLFFTIVSAIVDVKKGFVLLSNAGHPTPLMQREGSV
                     QFLEGKREALVGINMIHGDVSKIPFRNGDRLLLYTDGLIEVMNEKGEMYGEERLKNLM
                     ERSSDTDIRSASLNLKSSYESFKGSALAEDDITALLIEF"
     gene            complement(4941..7955)
                     /locus_tag="PKL93_09535"
     CDS             complement(4941..7955)
                     /locus_tag="PKL93_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ71538.1"
                     /translation="MIIQIISLFVGVAVIAILVSMIRNPIILKIGYRNIYRRKSDTFL
                     VIVGSLIGTALIMGSMAMNDSFQNFLYSQIERTQGEIDELVYIPSDNQNIGKELIPNS
                     KIEILVDSLLKNEQVDGVLPVLSRTVSIGLPGEARSQTGKSFQVSMIGVKADQLSKWP
                     DVDKVDLVLPSPTDELPEVVINKELAEAAGVAVGDTLEILVDPGQRLLFWIPLPEVRV
                     AEIVEGSGILHYQIETQGSNGFTMLMDVEEAREVLKIGVEDYYNALIVSNRGDFLAGE
                     RLTDQVVASIKSLVGEEVVVREVKKDSLSMVDQGNIGLLFLMLSVFAIFAGVLLLTNI
                     YLMLAQERRTELGTLRAIGYSRKKVSRTILYEGFFYSIFSSGIGVLAGLGIARFILGS
                     FVNLFEDAVSLIPFEGANIAFNSMQNSFVFFVRIDSIAYGFLLGLIIPMIIIVYTGRK
                     ISRTNIVTAVRNIPEELDERKRLLLNVIAAVGVLVSVVMAYSGYSLGNATGFFTGVML
                     AGLLIPVAIPMKNKRWIESFFSIAVIVFTMFSNSFDLIASNSGSSIYLTIAKGAAILF
                     AGLFLIVYNLKTFEYLLNKLFQKARAAAPVFKISIAFSARNRLRTGLTIAMFAVVIFV
                     ITLISIIPYSMEQMLVKSRDAIFAGFDAGAFSFTNESAITLTELKSQLEVREISTVSG
                     INVALRKDGRYDLEQVFALDDNFIDYNRMTELDFVEGLGISDVKDLWNYLRDNSGTVI
                     VSNSVLPDVRPGDVLELRGIADQGDAGNGAAAFTQKKLSSEMIDSGPIYLEVIATIPE
                     NTISFLNGLLIYIGNTPAELTGSSAARHFLFNLSGDTEVEKKANFDSLQDKISSRSPF
                     LLYVDDIMNLTSTMLQGTISILRSFLYFGMLVGIVGIAIIMFKALHERKRIIGMLKAI
                     GFTKAMVFSSFLLETSFIAIIGILLGMVTGTLTSVEIFASPLMEGMKLYIPWDQLISM
                     TLMFYIASLVSTIVPSYSASKIAPAEALRYFE"
     gene            complement(7958..8656)
                     /locus_tag="PKL93_09540"
     CDS             complement(7958..8656)
                     /locus_tag="PKL93_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71539.1"
                     /translation="MAFVEVRDLSKTYRSGEVLVEALKGVSFDIHEGEILAILGPSGC
                     GKSTLLNCLSGIDTPTEGKVTVKGVDLHSLKDDEKTRFRAMNMGFVFQFYNLIPVLKA
                     VENVELAMLTMGSNEKKAREAAMEILAKVNLREREHYLPSRLSGGERQRVSIARALVH
                     KPAIVWADEPTGALDTKTSNDLMNLITELNETFNQTFVIVTHDERVSAFSNRVLHMDS
                     GSILKIEENKELKV"
     gene            complement(8687..9223)
                     /locus_tag="PKL93_09545"
     CDS             complement(8687..9223)
                     /locus_tag="PKL93_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71540.1"
                     /translation="MYYLASAAILFVLNFSKGAYFHPVFFFLPFLIIVDYLIVLGIPG
                     RSYSLRVSAFLRNIQSILTLRRTFDESTKGKIIDSENLRNLEKVVSSLEEKLKKPSEL
                     QRKLYIFSAYVAPLFPLAVMLSSVIVQRRVEIVAGLFSYVASLVIVLLSRKAFSNLEK
                     TIEKLNGEIRKAVDDIAQ"
     gene            complement(9237..>9844)
                     /locus_tag="PKL93_09550"
     CDS             complement(9237..>9844)
                     /locus_tag="PKL93_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNQ71541.1"
                     /translation="RWGAKAISSDGKDLGKVIRVVIHPKNNEVTHLVIEKGIFNRVAK
                     LVPIATVFFAAPDEIRLRIESKDVETLQDYEETFFVTGEEIENLESGVTPVYWLRPVG
                     DYAELYPLPPLNTSINVPKDSKSLEPGCDIVTAEEKIVGRVKSFVLNDEGKITHLIGE
                     CGGFGSRSKKLIPIDWVEEIDECKVRVSASSVMVEKLPEMD"
BASE COUNT         2797 a         2409 c         1840 g         2798 t
ORIGIN      
        1 atatgccaat ctggaactcg gtacggccaa gtcctttcca gtagttctga atgtaaaaga
       61 atatgcaggc actgaatgca agtattgtct gaacaaaaaa gataagaaat gcaacctttc
      121 tatctctagc gattattgag aagctatcag caatttgctt caggaagccc cggaaggttt
      181 ttgagttacg gcccacagac tcgaggattg gtggttctgt gaacgaaaga gaatagaccg
      241 ccgaaaatgc gatgatcccg gcggaaaccc aaaatgcata gtgataattg tacgttccca
      301 ggaacccgcc tagtagaagc gccgcaactg aagacacttg aaatatggcc tcctgcctcc
      361 caagaaccga tatgaaattc tcttcagttt tcagatattt gagagaatca tatacaagag
      421 cctctccaga acccgattcc aggttatacg aaagagcagc tagtaccatg aaaatggtga
      481 aatgaaagaa gctggtcgca ccgatcaaca ccagaatact cgcaagagag agacatctac
      541 caatagttct gctgagcttt ctaccgaaca agtctgctac agacccggta gggacctcca
      601 tcatcaaaga ggtcacgtgg aatattcctt caaggaaacc gatctgcgtc aaactcattc
      661 ccttgaaggc catgtagatc atccatagac cgctcgacag tctcatgttc ataaggaaag
      721 agaataaata attcttcttt atgttgcttt tcattcgctc aaccatatta ttcataacat
      781 tttctcccaa aagaaagagg ccgtgacgct cgtcacggcc tcttgattca cagctaagat
      841 ttcagagcaa cccaggctcg acgagatagc attcacccac ggaactcgaa aatccgccca
      901 aaatagctct tctataggcc tgaataactt ctagcatgtg ggttcgctcc ttctcctgga
      961 ttgagttacg aagattatac atcaaatagg ctaataaaaa aactcccgat gtggatgagg
     1021 tatttcttgc aattctctgg gttgttcagc cgctggaaaa actccaccgg tgccgagctc
     1081 tcaatcttct tcctcagact tatgaaggag attttttgag cttctcctga agatcgactc
     1141 atcgtaggag tccgcaagaa tctcttttgt gcgggtaaag gaatcgatat caatttcctc
     1201 cgaatactcg aagctcttca gcataccttc ttcaatggtg agaatttttt cgccatgttc
     1261 tgcaatgaac tgcctgtcgt gactaatgaa gaaaaaccct cccgagaagt cgtttagaag
     1321 tttgtcaagt acttcgagag attcaatatc caaatgattg gttggctcgt ccatgaagat
     1381 gaactcaacc tttgaaagaa gagattttag taaggcaagc ctcatcaact ctccatagct
     1441 catcgtttta atctccgaga aagccctctc tctcttgaga ccgatattgg ccaggtgact
     1501 tcttactgcg ggctcttcca gttcaaggtc ggcaatttct tcatagggag tttttctttc
     1561 gaagagttct gcgacattct gtggaatgta catccagttt acactctcat ttctgtagat
     1621 tttgccactc gatagttcca gaattcctac aaggcattta atcagagttg ttttccccga
     1681 accattcggc ccaatgagac cgactctatc ggaagtgtct atgttaacag aggcctccct
     1741 tatgacttca ttttctccgt aagagaacga caggctctcc accatgacca tttttctttt
     1801 cggaactcca tatttgggaa tcctgacatt aactggcttc tcaacaaacg gtttttcgca
     1861 ctccagttca ccgatcagtc tttcagtcct tttctgtacg gctaacgccc gtttggctag
     1921 tttggccgcc atatgtcctt catggccttt atcgcttgca aatctctttg aagcctctct
     1981 tgaattggac cagctctttc tcctggtcgc atcgagttcc agttggcgta tcttcttact
     2041 tatttcttca cttgttttct gtcttgagag gaaatctctt tcaagatgcg ccagcatctc
     2101 agaataacct cctcgaactc ctactgcctc tccccttttc agacagatcg tcttgtgagc
     2161 gactttgtcg atgaatctcc tgtcatggct cactacaata actccaacac ctctctcgct
     2221 tagtgaggta atgatctctt caagtctcaa ggtcatttct atatcgagat ggtttgtcgg
     2281 ctcgtcaagc aagaaaagat tcgaaccgga gagaactccc ctaatcagtg agagaattct
     2341 cttctgccca tgggaaagat tcatgtacgg gaaatccagg ttgtcaacat cgataccaaa
     2401 ctcatttaga gatgaagaaa tcttcgtttc gaactcgaat cccccaacat tatgaaattc
     2461 atttatgagt tccgcataag ccataggatc atttacagac ccacccaatt tctcgtagat
     2521 atcgaagagc tcttctctcg ccccgagaag atactccttc actgtcccat gaaagtctga
     2581 cgtctcctgg ttcacaagaa gcggtttgat accttgctct atagttattt tcccatccga
     2641 gggagtgatt tcacctataa ttgattttag gagagtcgac ttgccggcac cgtttgcccc
     2701 ggtgacagcc aaaacctctc ctgcagcgag atcgaaactg attttcttaa gaacgtctac
     2761 acttccaaag gaaattgaaa gattgtatat tcgaagcaaa gtgatcacct ccagtaaggc
     2821 ttaaagctag aaatcattca aattgaaagg tcaaatagaa tcgaagtctt tcgatgcttt
     2881 tttgaggaag tattcagttt tgtcgattaa agaagtctat tcttcatttc agcacctgct
     2941 ggtttgtgat cttttattta tatcacatcg aaaccaactt ggtcaaacag cagctgtact
     3001 gccagtacta gatagtgatc ttcaaaaggt ttttcccgtt ctcgtaacga taatcaaccc
     3061 tcttcgaaaa cctctttatc aaatagacac ccatcccgcc cttttgcaaa agttctatgt
     3121 tctcaagatt tggatcgtct acctcaagag ggttgaactt tgtggaggca tcttcaatgg
     3181 tgattgtcac tgcttcttct ccaacttcga ggtttaccga aataagatca tcttctttga
     3241 gctcaccgtg cttgacaatg ttcgagcacg cttctattac tataagccct gctctgaatc
     3301 ctgtttcatt tcctttagtt cttttgacta tctcggacac ttttgcagaa aaagcatcaa
     3361 tcttatatag atcgggttcg accgtaaaac tgtagttctc tttggccatt ctttcaacac
     3421 agatccttga tggcctctgc aacgctgcag tccttaacga tgaagagcct gtcaagtcgc
     3481 aaagtattca gcagtttctt tatctcttca gagagattca ccagaatcaa atcacttcct
     3541 tttgcggaaa ccgtcttgta aatgcttatg agagctgcaa ttcccatgga atcaatgaat
     3601 tcgatgttgt tgccatccac aataactgtt gagcaacttt tcagatcaac agtctcttgc
     3661 cattttctga aatttgccga catttccccc gtaattcttg ccctactatc aagggaaatt
     3721 actccgatcc catcgttagc cttcttgaat tcgaacattc ctcactcagc tcctcatcct
     3781 agactttacg acaacagctt atcgaactag aactctatca gtaaggccgt aatatcgtct
     3841 tccgcaagtg cactcccctt aaaactctca taagaggatt tcaagttcaa agaggcactc
     3901 cttatatcgg tatctgaact cctctccata agattcttca gtctttcctc accatacatt
     3961 tcacctttct cgttcattac ttcgatcagc ccatcggtat acaaaagcaa tcgatcaccg
     4021 ttacggaaag gtatcttaga aacatctccg tgaatcatat ttattccgac aagagcttcc
     4081 ctcttccctt caagaaactg tacagatccc tctctctgca tcaaaggagt cggatgtccc
     4141 gcatttgaaa gcagaacgaa gcctttctta acgtccacta ttgcggaaac aattgtgaag
     4201 aataggttga acctctcaaa aatgaaattt ctatcaagct ccttcatgaa actgctcaaa
     4261 tcaaactcac cgtcatcgat actggtcctg aaatcttcaa tgaacctgtg taccattacc
     4321 gatagcattg ccgcatttac gccgtgtcct gacatgtcgg cgagataaac aaaataccga
     4381 ttctcatcca acctgaatac atcaaagata tccccaccaa ctctgtaaga gggctcatat
     4441 atccattcaa ctgagagtcc ttgaggatta cttttcggaa caagagcatt ctgaatttta
     4501 cccgctgcta tgacatcgct ttcaagatta tccaccgcca ctctcaagga tctgataaga
     4561 tcatggagct tcagatgcgt attcaccctt agcagaacct cttcggggag aaagggcttt
     4621 gaaatgaaat ctactgcacc ggaactaaga gctgtgagct tgctttctct gtcggcggac
     4681 cctgtaagaa caataacggg tataggctct agttcttcac tttctttgag ctgagctagt
     4741 atatcaagtc cattgtagtc aggcaacatt agatcaagaa gtataatatc cggcttctgc
     4801 acagccagcg atgagaatgc cgacctgcca tccgcacttt ctacaacgtc atggttgtct
     4861 ctcgaaagaa tcgatttcag cagaactcta acagacaggt catcatccac taccagtatc
     4921 ctactcaacc ggatcacttc ctattcgaag tatcgcaaag cttctgcagg tgcgatttta
     4981 gaagccgaat aactgggaac aatagtcgaa acaagagagg caatatagaa cataagcgtc
     5041 attgagataa gctgatccca aggaatgtaa agcttcatgc cctccattaa aggagaggca
     5101 aagatttcaa cgcttgtaag cgtaccggtc accatcccaa ggagaattcc aatgatcgcg
     5161 ataaacgatg tctcaagtaa gaaagaagaa aacaccattg ctttggtgaa accaattgct
     5221 ttgagcatgc cgataattct cttcctttca tgaagagctt tgaacataat gatcgctata
     5281 ccaactatgc cgacaagcat tccgaagtac agaaaagacc ttagaatgct tatcgtccct
     5341 tgcaacattg ttgaggtcag gttcattatg tcatctacgt atagaagaaa aggacttcta
     5401 gatgatatct tatcctgaag cgaatcgaag tttgcctttt tctctacctc ggtgtctcct
     5461 gaaaggttga aaaggaaatg tctcgcggca ctgctgcctg tcaactccgc aggagtattc
     5521 ccaatgtaaa tgagcagtcc attgagaaag gagatggtgt tttccggtat cgttgctatc
     5581 acttcaagat aaatggggcc ggaatcaatc atctctgaag atagcttttt ctgcgtgaaa
     5641 gcagcggcac cgtttccggc atcgccttga tcggctatcc ctcttagttc aagcacatcc
     5701 cccggtcgaa catccggcaa aacactgttt gaaactataa cagtgcccga attgtctctc
     5761 agataattcc acaagtcttt cacgtctgaa atcccaagtc cttcaacgaa atcgagttcc
     5821 gtcatcctgt tatagtcaat gaaattatcg tcaagcgcaa agacttgctc caaatcatat
     5881 cttccgtctt ttctcagtgc aacgtttatc cccgacacag tcgaaatctc cctgacttcc
     5941 agctgagatt tcagttcagt caaggtaatt gcactttcat tagtaaaaga aaaagctccg
     6001 gcatcgaagc cagcaaagat tgcatctctg cttttgacga gcatctgttc catagagtag
     6061 ggaattatcg aaatcagggt aataacaaaa atgacaacgg caaacatggc aatagtgagg
     6121 cctgttctca aacgatttct tgccgaaaat gcaattgaaa tcttgaatac aggagctgcg
     6181 gctcttgctt tctgaaagag tttgttcaaa aggtactcaa aggtcttcaa gttgtagaca
     6241 atcaaaaata gcccggcaaa aagaattgct gcaccttttg caatcgtgag atagatggat
     6301 gagccactat tcgatgctat tagatcaaag gaattgctga acattgtgaa tacgattacc
     6361 gctattgaga agaaagattc tatccatctt ttgttcttca tcggaatcgc aaccggaatc
     6421 agcaatcccg caagcataac gccggtaaaa aagccagtcg catttcccaa cgaatatcca
     6481 ctgtaggcca tcaccactga aacaagaacc cctacggctg ctatgacatt cagcagaagt
     6541 ctcttccttt catcgagctc ttcaggtata tttcttaccg cggtcactat attcgtgcgt
     6601 gaaatctttc ttcccgtgta aacaatgatt atcataggta tgataagccc caaaagaaat
     6661 ccataggcaa ttgaatcgat ccgcacgaag aacacgaatg agttctgcat ggagttaaaa
     6721 gcgatatttg caccttcgaa aggtatcagt gagacagcat cctcaaatag gtttacgaaa
     6781 ctacctaaaa taaacctcgc aattcccaac ccagcaagga ctccaatacc tgacgagaaa
     6841 atagaataga aaaaaccctc atagagaatc gtcctggaca ctttctttct tgaataacca
     6901 atggctctga gggtaccaag ctcggtcctc ctctcttgag caagcatcaa gtaaatgttt
     6961 gtaagaagaa gcacacctgc aaatatggca aagacactga gcatgaggaa taagagaccg
     7021 atgtttccct ggtcaaccat actcaaggaa tccttcttga cctccctgac cactacttcc
     7081 tcaccaacta aggactttat gcttgcaaca acctgatctg taagcctttc acctgctaga
     7141 aaatccccac ggttcgaaac gattagtgca ttataatagt cttcaacacc tatcttaagt
     7201 acttctctgg cttcttcaac atccattaac atggtaaatc catttgaacc ctgggtttct
     7261 atctggtagt gaagaatccc actcccttca acgatttcag ccacccttac ctcaggaaga
     7321 ggaatccaga aaagcaatct ctgcccaggg tctacaagaa tttcaagagt atccccgacc
     7381 gccaccccgg cagcttcggc cagctctttg ttgataacga cctcgggaag ctcatctgta
     7441 ggagagggga ggaccagatc cactttatcg acgtcaggcc attttgagag ttgatcggct
     7501 tttactccta tcatgctcac ctgaaaactc tttccagttt gacttctggc ctctccgggg
     7561 agtccaatag agacagttct actaagaacg ggaaggacac cgtctacctg ttcgttcttc
     7621 aaaagcgaat cgacaagaat ctcgattttg gaattgggaa tgagctcttt accaatgttt
     7681 tgattatctg aagggatgta gaccagttca tcgatctcac cctgagttct ttcgatctga
     7741 ctgtaaagga aattctgaaa agaatcattc atggccatcg accccatgat aagcgccgtt
     7801 cctatcagag atcccacaat caccagaaaa gtatcggatt ttctcctgta aatatttctg
     7861 taaccgattt ttagaattat cggattcctt atcattgaaa ctagtatcgc aatgacggca
     7921 acacccacaa agagactaat tatctgtatt atcactgtta caccttcaat tctttgtttt
     7981 cttcaatctt caaaatgcta ccactatcca tgtggagaac tctgtttgag aatgcagaaa
     8041 ccctttcgtc gtgcgtaaca ataacaaaag tctgattgaa cgtttcattc agctcggtga
     8101 tgagattcat aagatcattg ctggttttcg tatccagggc tcccgtaggc tcgtcggccc
     8161 atacaatagc cggcttgtga acgagcgccc ttgctattga gactctctgt ctctcgccac
     8221 cactcaatct tgaagggagg taatgttctc gctcccttaa attgactttt gcaagaattt
     8281 ccatcgcagc ttcacgggct ttcttttcat tacttcccat tgtgagcatc gcgagttcca
     8341 cattttcgac tgcttttaag acaggtatca gattgtagaa ttgaaagaca aaacccatgt
     8401 tcattgccct gaagcgagtc ttttcatcat ccttcaaaga gtgaagatca acccccttga
     8461 ctgtaacctt cccctccgta ggcgtatcta ttcctgaaag acagttcagg agtgtacttt
     8521 ttccacatcc ggacggtccg agaatcgcta gtatctcgcc ttcatgaata tcaaaagaaa
     8581 ctcccttgag tgcttcgacc aacacctcac ccgacctgta agttttacta agatctctga
     8641 cctcaacaaa tgccattttg aatctccttc caataaaaac tctcaattat tgagcgatat
     8701 cgtccacagc tttcctaatt tctccattca gtttttcgat agtcttttca agattggaaa
     8761 aggcctttct tgacaagaga acaattacta acgatgccac atacgaaaaa aggcccgcca
     8821 caatttcaac tcttctctga acaatcacgg aggataacat aaccgcgagc ggaaaaagcg
     8881 gcgctacata agctgaaaaa atatacagct ttctctgtag ctcagatggc ttcttcagtt
     8941 tttcttcaag tgaggaaaca accttctcaa ggtttctcag attctcagaa tcgataatct
     9001 taccttttgt actctcatcg aatgttcgtc tgagggtgag tatggattgt atatttctca
     9061 gaaaggctga aactcttagt gaataactcc tccccggaat acctaaaact attaaataat
     9121 caacaatgat taagaaaggc aagaaaaaaa agactggatg aaaataggca cccttagaaa
     9181 aattgagcac gaataaaatc gcggctgatg ccaaatagta caccgtcact ttttcatcaa
     9241 tccatttccg gcagtttctc taccataact gacgatgctg aaactcttac tttacactca
     9301 tcgatttcct caacccagtc tatcggtatc agtttctttg acctagaccc aaatcctccg
     9361 cattcaccta tcaaatgggt gatcttacct tcgtcattta gaacgaagct ttttaccctt
     9421 cccacaatct tctcttcagc tgtaacaatg tcacatcccg gctcaaggga tttgctgtcc
     9481 tttggaacat ttatcgaagt gtttaacggc ggaagagggt atagctctgc gtaatcccca
     9541 actggtctta accagtaaac cggagtaact ccgctttcga gattctctat ttcttctcca
     9601 gtaacgaaga acgtctcttc ataatcctgc agtgtttcaa cgtctttcga ttcaattctc
     9661 aacctaattt cgtcgggagc tgcaaagaac acagtagcta ttggcaccaa ttttgccact
     9721 ctattgaaga tccccttttc tatcactaga tgagtgactt cgttgttctt aggatgaatg
     9781 actacccgaa tgacttttcc gaggtctttg ccgtcggaag aaatcgcttt ggcgccccat
     9841 ctga
//
LOCUS       DAOQYI010000053         9772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_7432_length_9772_cov_59.467428, whole genome shotgun sequence.
ACCESSION   DAOQYI010000053 DAOQYI010000000
VERSION     DAOQYI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7432_length_9772_cov_59.467428"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKL93_09555"
     CDS             complement(<1..211)
                     /locus_tag="PKL93_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HNQ71542.1"
                     /translation="MDKVVKEIAEIIDEKLGEDIVILDVSKVSNLADYFVVTTANSDP
                     HMDSLREAILQYIEREAVEIIYYDKG"
     gene            complement(552..2378)
                     /gene="glmS"
                     /locus_tag="PKL93_09560"
     CDS             complement(552..2378)
                     /gene="glmS"
                     /locus_tag="PKL93_09560"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNQ71543.1"
                     /translation="MCGIVGMVGKDLTIRKLVDALKKLEYRGYDSAGVAVNSRDGLRV
                     MKAVGMISSLEKLLGDDLDSSVVQGIAHTRWATHGGPSDFNAHPHTDCTGKIAVVHNG
                     IIENYDVLRLDLEKKGHIFKSVTDTETIAHLIEDHYSGDIISAVRHALLDLEGAYAIG
                     VVHQDHPDVIVAARKGSPLVVGSTGESGFLASDVTPLLKYIRDVYFVDDGEFVVIRPE
                     GISISRMDGTSVRKPSTKITWSEDSAEKSGYEHFMLKEIFEEPQTLRNALMGRIRGGV
                     PNFNEIEHLREEIEKARSLTILACGTSFHAGLVFQRFLQDYTEIRAEVEVASEFRYRR
                     LQEGFSDLVVAISQSGETADTLEGIRKAKKLGTKIISLTNVVGSTISRESDAVIYINA
                     GPEIGVAATKTYVAQLAVLLLLGAAIADIAGRSGFDIPKIVNELEGMPTVFENTLPTA
                     DEQCKRLAAEHSDYKHFMYIGRGYSYASALEGALKLKEISYIHASGYQAGELKHGPIA
                     LLDKDFPVFAIVPEDELKSKMISNIMETRARDAKVLALCSENDKEVAKSVNSRIEVPR
                     VLDPIYPLVMSPYLQLFAYYVAVKKGLDPDKPRNLAKSVTVE"
     gene            complement(2380..3378)
                     /gene="plsX"
                     /locus_tag="PKL93_09565"
     CDS             complement(2380..3378)
                     /gene="plsX"
                     /locus_tag="PKL93_09565"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNQ71544.1"
                     /translation="MQSIRIALDAFGGDNAPQVNVDGAFLALKEFQDLEILLVGREED
                     LKPLIKESPETSRISIVDAREVFPMSEKPSLLLRKKETSLYKAAIQVKDANADALVSA
                     GNTGGVLAAALFVVGRIKGVDRGAIAVLVPSKNGFTILIDAGANAEARAEHLRDFGTM
                     GYEYAKLMGREKPKVGLLNVGEEQEKGTELTKLAFDYLKNDLGDSFAGNVEGRDINYG
                     DVDVVVCSGFDGNIAMKTMEGTGKLISATLKKEIKKSGLFGIIGALFLKRALGRLKKT
                     MDPSEYGGAFILGVKGAVVKAHGNSNALAIKNAIRVAYQGVKGDLIKKLEEKLGGK"
     gene            complement(3382..3564)
                     /gene="rpmF"
                     /locus_tag="PKL93_09570"
     CDS             complement(3382..3564)
                     /gene="rpmF"
                     /locus_tag="PKL93_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNQ71545.1"
                     /translation="MAVPKQKRSRSRTHLKRAKMYSSIKVAVSTCPNCGEPKQPHRVC
                     LHCGYYGGRQILEIGE"
     gene            complement(3582..4118)
                     /locus_tag="PKL93_09575"
     CDS             complement(3582..4118)
                     /locus_tag="PKL93_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNQ71546.1"
                     /translation="MAKIHELIVDLNSFEFKKTIDLVLDPGFTDEIKLLSNVDVHIEL
                     YKDKDSIIVTGSVKTVVEDRCARCLKVVSIPVEGTVEATYVQRDSLPLVREGAGDDLE
                     NLLPLEGDLLDLSDRVIEAIIVEVPQKVLCKKDCAGLCPFCGADLNEEPNHSCQKKEE
                     MPDGWHKAIAELKGKLKS"
     gene            complement(4177..4866)
                     /locus_tag="PKL93_09580"
     CDS             complement(4177..4866)
                     /locus_tag="PKL93_09580"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71547.1"
                     /translation="MHLRISSLSKAFDGETIVDKFSYSVEGDFFLTILGSSGCGKTTL
                     LRMISGILKPDAGRVELQSERLGFVFQDDRLIPWLNAFQNISIVSPKSNPVEYLSFVG
                     LKGHENKFPSQLSGGMRRRLNIARALAFNPDLILMDEPFNSLDVVIKDRLIEDIQKLW
                     TGRKISIIMVTHDPSEAARLSTDIILVQDRFSKVDEIKLGDPLERSPDDIDLISRDLL
                     SRMKKFSSYLH"
     gene            complement(4850..5584)
                     /locus_tag="PKL93_09585"
     CDS             complement(4850..5584)
                     /locus_tag="PKL93_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71548.1"
                     /translation="MAPIVSVGFLLLGWFIASVIVSSDLLLPGPLETMEEFLRIISSR
                     KGWSDIAETCAKAFLGLFIALGLGLAVGFVMGLIDVLYELIKPIVVILQSIPIVSWLA
                     LAIFWWGVGFSSPVYIVFLTLFPIFTINIAEGVRNVDKKLVEMAKIYKIPRNTVFGKI
                     YLASAFPFIVSSMRAGIGIMWKSVAVAEFMVGASGIGRAMSDAKYSINIERVFAYTLV
                     LVVLGILTERLLDMIIKRGSRYALKN"
     gene            complement(5595..6515)
                     /locus_tag="PKL93_09590"
     CDS             complement(5595..6515)
                     /locus_tag="PKL93_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71549.1"
                     /translation="MRKFVAVSTLLFITLFSFGVNYINPVGPTLIPIAELLSSNLSEE
                     IPGLEINLWRSVDEAISMMVTEKAQIALLPVTVGVKLASSGVDIKLAAVSMWNGFYFI
                     STEKTIESIEDLVDREVYTLQAPGQTADTILRGALESKGYEVGRDLDIVYVGGAEAVQ
                     LLAGGKAKVILVPEPFASLAETRVQGSIRSMPIEDLWESFNEQRINIPTSGIFVSGTL
                     DRGVAESFLLLYQQSMNLSLTNREKTAATVSEKMGGFPAPVLQKAMDTAGFLFLTAED
                     AREDIAIYLNRLKELDPELTGDFDIDALFF"
     gene            6732..7004
                     /locus_tag="PKL93_09595"
     CDS             6732..7004
                     /locus_tag="PKL93_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ71550.1"
                     /translation="MNKKELIAEIAEKTGVTKKDAGKTLDTVIDIIEKTLSKGDVVRL
                     VGFGTFMVASRKARKGVNPRTKKPITIPGGKVPKFVPGKELKEKVK"
     gene            7053..7949
                     /locus_tag="PKL93_09600"
     CDS             7053..7949
                     /locus_tag="PKL93_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HNQ71551.1"
                     /translation="MKDLKGLWFFLIVLIIVVALGSFFNIYFLKQFGDEMISSLSTRE
                     IESKLQLMSERVSLLNTNLDSLSSVEVREDLSRIITDFERIIADINYVSNSLSSNPVL
                     DSIAQLSSDLKLIKRSIDSLDSGESVDYSTQIEGVRGKIVSLEWQVEELKKLLESSTA
                     DLKKSVMNVNAFSSQADQSYKEEVGKGVRIRIESGFKGSSILHDSDILMILNELYALR
                     ATEISLNGKRIMPYTYVRCVGATVIINDEPTQISPIVIEVLGEYDYLVSGLGLLKEFF
                     AGREIDMTFLPLEFITIPAGGG"
     gene            7955..9772
                     /locus_tag="PKL93_09605"
     CDS             7955..9772
                     /locus_tag="PKL93_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNQ71552.1"
                     /translation="MRKSALLFLLVLIMVFFTGCDFPFITRDDFTQRMDALEQKIDSL
                     AAGQKKLAQLEDAVEELAFRINFVQAIEPTYAGYEELEAVRAELQIIKNMLAESVIDS
                     ASSAAIVKTLYDKIDGVLTSTVENNIITREIDDLNRKVQALESLNSSRYSELVQRISE
                     IGTYDSSIDEAKYEELLQRIEEIKSAEVVVVQQESDEDDRRYEELVNKVEELAARTDI
                     SVLDSAKYDSLARRLAELEQTVGSSVIDSEVFNELVSRIDEMDSRTRELADNLQTIER
                     QEEPEEKNFVTMATFLGEISDIRSRLGAAAYETIEPTSHVSYIVKSGDNLWSIAQAYG
                     VTVEQLKAMNPEIKNWDLIYRGDEIKIPLSLDNLMAKASIATHFGLNLGVDFLVDSIE
                     SNFGSYDYGYANPGMDLTVPPGSRITAFLPGKVILSERVNDLYGEMVVVDHGNNMKTV
                     YARLGSRMVMKGDFVRVGDTIGSASDAKGNLHFEFWKADVPVNPADIIFENVGTFEVT
                     MYTEWDDAKNPTSPSFKMTASGDFVKGYRTVAADPVVIPLGSIVYIPFFSSSPNKGFF
                     VVEDTGSSIKGNKIDVYTHDFDTASNFKEDLLVYVVKKP"
BASE COUNT         2540 a         2368 c         2171 g         2693 t
ORIGIN      
        1 tgcctttatc gtagtagata atctctacgg cttccctttc aatgtattgc agtatcgcct
       61 cccggagcga gtccatatga gggtcggaat ttgccgtagt caccacgaaa taatctgcaa
      121 ggtttgacac cttcgagaca tcaagtatta ctatgtcttc tccaagtttt tcgtcaatta
      181 tctcagcaat ttccttaact actttatcca agattgattc ccccattata gacacgttct
      241 cagttctccg ttgaccgtcc gcctaattca caaaattctc aagcccactt cactgatcat
      301 gaatagacac ttgcccatga acgaatagcc gatcttcctt tcctaacttc cttactactc
      361 ctgaaggtcc ctttgatcga acaaacgacg agtatccaca gaggaagcac tttctgaaga
      421 tcacactccc ccggttcaag caggactcag tagatttctt tcgctttcat tccagagtag
      481 tagcttcaac tcatatgttt catacaatgc ctgtttcatt tatcatgtta cactaatcgt
      541 cagatttaac tctattcaac tgtgacgctc tttgctagat ttctaggttt gtcgggatca
      601 agtcccttct tgacggcgac atagtatgca aagagctgta gatagggtga cataacgagc
      661 ggataaattg gatccagaac tcgcggcacc tctattcggc tgtttaccga ctttgctact
      721 tccttgtcat tctcggagca aagagccagt accttggcgt ctctagcccg tgtctccatg
      781 atatttgaaa tcatcttcga cttcagttcg tcttcgggga caattgcaaa gaccgggaag
      841 tctttgtcaa gcaacgcaat cggcccatgt ttcagttctc cggcctgata accgctggcg
      901 tgaatatagc ttatctcttt gagcttcaaa gctccttcaa gagcggatgc gtagctatat
      961 cctctaccta tgtacatgaa atgcttataa tccgagtgtt cggccgccaa tctcttacac
     1021 tgctcgtctg ctgttgggag agtattttcg aatacggtgg gcattccttc cagctcgttt
     1081 acgatcttcg ggatgtcgaa cccgcttcta ccggcaatgt cggcaatggc cgctcccagg
     1141 agaagcaaga ccgccagctg ggcaacataa gtcttagtgg cagcaacacc tatctcggga
     1201 ccggcattta tgtagattac cgcatcgctt tctcttgaga tagttgaccc aacgacattt
     1261 gtaagggaaa tgatctttgt tccaagcttc tttgctttcc taatgccctc aagagtgtct
     1321 gccgtctctc cggattgtga aatcgcgacc acaagatcgc taaacccttc ctgcaatctt
     1381 ctgtatcgaa attctgaagc gacttcaacc tcggctctta tttcagtgta atcctgcaga
     1441 aatctttgaa agaccaatcc ggcgtgaaaa ctcgtaccac atgcaagaat agtgagcgat
     1501 ctggccttct ctatttcctc tcttaaatgc tcgatctcat tgaagttcgg cacgccaccc
     1561 cgtatcctgc ccatcagggc attcctcaaa gtctggggct cttcgaatat ctccttgagc
     1621 atgaagtgct cgtaaccgct cttttccgca gagtcttcac tccaggttat cttggtggag
     1681 ggttttctga cgctcgttcc gtccattctg gatatgctta tgccttccgg gcgaataacc
     1741 acgaactctc catcatcgac gaagtatacg tctcttatat acttcaataa aggtgtgaca
     1801 tctgaggcga gaaacccgct ctccccggta gatcctacaa cgagaggact gccttttctt
     1861 gctgcgacaa ttacgtcggg atgatcctga tgaactacgc cgatagcgta ggcgccttca
     1921 agatccaaca gagcgtgtct gactgcggaa attatatctc ccgaatagtg atcttcgatg
     1981 aggtgggcga tagtctctgt gtcagtcaca gatttgaaga tatgcccctt tttctcgaga
     2041 tcgagacgaa gaacatcgta gttctcgata atcccgttgt gcacaactgc aatttttccg
     2101 gtgcaatccg tatgagggtg cgcattgaaa tcggaggggc ctccgtgagt cgcccatctg
     2161 gtgtgggcaa ttccctgaac tacactcgag tcgagatcgt ccccaagaag cttctcgagg
     2221 gaggaaatca tcccaacggc cttcattacc cggagaccat cacgactgtt cactgcgacg
     2281 ccggcagaat cataacccct gtattcgagc tttttcaagg catcgacaag cttccttata
     2341 gtaaggtctt tccctaccat cccgactatt ccgcacaaat cactttcccc cgagtttctc
     2401 ttctagcttt tttatcaagt cgcctttcac tccctggtaa gcgactctga ttgcattctt
     2461 tattgcaagg gcgtttgaat ttccatgagc cttgacaacc gctcccttga ctccgagaat
     2521 gaatgcgccg ccatattcgc tgggatccat cgtttttttc agtcttccga gcgcacgttt
     2581 gagaaagaga gcacctataa ttccaaaaag accacttttc ttgatctctt tcttaagcgt
     2641 ggcggaaatc aacttgcccg ttccttccat agtcttcatc gcgatattgc cgtcaaaccc
     2701 gctgcaaact acgacatcaa cgtctccata attgatgtcg cggccttcga cgtttcccgc
     2761 aaaggagtcg cccagatcat tcttgagata atcaaaggca agtttcgtga gttccgttcc
     2821 cttctcctgc tcttctccga cattgagcag ccccaccttc ggtttttctc tccccatgag
     2881 ttttgcgtac tcataaccca tagtgccgaa atcccttaag tgttctgccc tcgcctcggc
     2941 atttgctccc gcatcgatca gtatggtgaa gccattcttg gaaggaacaa gaaccgcaat
     3001 tgctccacga tcgacgccct tgatcctgcc cacgacaaag agcgccgctg caagcactcc
     3061 accggtgtta cctgcagata ccagagcatc ggcattcgca tcttttactt gaatagctgc
     3121 cttgtataga gaagtctctt ttttcctcag caaaagagaa ggtttctccg acattggaaa
     3181 aacctctctg gcatctacta ttgaaattct tgaggtctca ggagattctt ttatcagagg
     3241 tttgagatcc tcttctctgc ctaccagtaa tatttcgaga tcctgaaact ccttcaaagc
     3301 aagaaaggca ccgtctacgt tgacctgagg agcattatct ccaccaaagg catcgagggc
     3361 aattcttatg ctttgcattt attactcacc gatctcaaga atctgtcttc ctccatagta
     3421 gccacaatgt agacaaaccc tgtgtggctg ttttggttca ccgcagtttg ggcacgtaga
     3481 aacagcgacc tttattgagc tatacatttt tgctcgcttc aagtgagtcc tgcttctgct
     3541 tcttttttgc tttggaacgg ccacgctaat ccctccaata tctaactctt caattttccc
     3601 ttaagctcag ctatcgcttt gtgccagcca tcaggcattt cttccttctt ctggcaagaa
     3661 tgatttggct cctcgttaag atccgctccg caaaacggac aaagacctgc acaatccttt
     3721 ttacagagaa ccttctgagg aacctcaaca attatagcct caattactcg atctgacaag
     3781 tccaggagat cgccttcaag tggcaagaga ttctccaaat catcgcctgc gccttctctt
     3841 actagtggaa gagaatccct ttgcacatac gttgcctcaa ctgttccttc aactggaatg
     3901 gatactactt tcagacagcg ggcgcatctg tcttctacga cggtttttac ggaaccggta
     3961 acgataatcg aatctttatc tttgtaaagc tcgatgtgca catctacgtt gctcaagagc
     4021 ttgatctcat cagtgaaacc cggatccaga actaaatcta tagtcttctt gaattcgaaa
     4081 ctgttgagat caacaatcaa ctcatgaatc ttagccatac aaatcacctc tgtgccagca
     4141 aatactacag caagggtatt gcaaaacact ctcgtttcaa tgaagataag aagaaaattt
     4201 cttcattctg gatagcagat ctctagagat caagtcgata tcgtcgggac tcctttccag
     4261 gggatctccc agtttgattt cgtccacctt agagaaccta tcttgtacaa gtattatatc
     4321 cgttgaaagc ctggctgcct ctgaagggtc atgggtaacc atgatgatgc tgattttcct
     4381 gccagtccat aatttctgaa tatcttcgat gagccgatcc tttatcacga catcaagaga
     4441 gttgaaaggc tcgtccatga gaatcagatc ggggttgaat gcgagtgctc ttgcgatatt
     4501 cagtctcctc ctcattccac cgctcagttg tgaaggaaac ttattctcat gaccttttag
     4561 gcccacaaac gagaggtact ccacaggatt ggattttgga gacactatcg agatgttctg
     4621 gaaggcgttc agccatggaa tcaatctgtc gtcctggaaa acaaatccca gcctctcgct
     4681 ttggagctcg accctacctg catccggttt cagtataccg cttatcattc tcaaaagagt
     4741 cgtttttccg caccccgagc ttccaagaat tgtgagaaag aaatcgcctt caacagaata
     4801 ggagaatttg tctactattg tttctccgtc gaaagccttg ctgagactgc taattcttaa
     4861 gtgcataccg actacccctt ttgatgatca tatcgagaag cctttccgtt aaaataccca
     4921 gaacaacaag aacaagtgta tacgcgaaga ccctttctat gtttatggag tattttgcgt
     4981 ctgacattgc tcttcctatc cctgatgctc ccaccataaa ctcggctacc gcaactgatt
     5041 tccacattat tcctattccc gctctcatgg aagatacgat aaaaggaaat gcagaagcga
     5101 gatagatctt tccgaagact gtattcctgg gaatcttgta gatcttggcc atttcgacaa
     5161 gcttcttatc cacgtttctt acgccctctg ctatgtttat tgtgaagatc ggaaacaagg
     5221 taaggaatac tatatataca ggggaggaga agccaactcc ccaccagaat attgccaggg
     5281 ccagccacga cacaattggt atagattgca ggataactac tatcggctta atcagctcat
     5341 ataatacatc gattagtccc ataacgaaac caacagcaag gccaagaccc agcgcaatga
     5401 acaatccgag gaatgccttt gcgcaagttt ccgcgatgtc cgaccaaccc ttcctgctcg
     5461 agatgattct tagaaattct tccattgtct ctaacgggcc gggcaacaac aggtcgctcg
     5521 agactattac ggatgcgatg aaccacccca gcagcagaaa gcctaccgag acgattgggg
     5581 ccaacgtcct ctttctagaa gaagagagca tcgatatcga aatcacccgt tagctctgga
     5641 tcaagttcct tgagtctatt aaggtagatc gcaatgtcct ctctcgcatc ttcggccgtc
     5701 aaaaagagga aacctgccgt atccattgcc ttttgcagta ccggtgcagg gaatccgccc
     5761 atcttttccg atacggttgc agcggttttt tccctgtttg taagagagag attcatagac
     5821 tgctgataca gaagcaggaa cgactccgca acgcctctgt cgagagtgcc gctgacgaat
     5881 attcccgatg tagggatgtt aatcctctgt tcgttgaaac tctcccaaag atcttctatt
     5941 ggcatcgatc ttatcgaacc ctgtacacgt gtttcggcaa gcgatgcgaa aggctcaggc
     6001 acgagaatta ccttagcctt gccccccgca agcaactgaa ctgcctctgc cccaccgaca
     6061 taaacaatat ccaggtctct tccgacttcg tatcccttcg actcgagagc accgcgaaga
     6121 atagtatctg cagtctgtcc cggtgcttga agagtatata cttctctatc gaccaggtcc
     6181 tcaatgcttt ctatcgtctt ttctgtagat atgaaataga aaccgttcca catgctgacg
     6241 gctgctagct ttatgtctac accggacgat gcgagtttaa cccctacagt gacgggaaga
     6301 agggcaattt gtgccttttc ggtaaccatc atcgaaatag cctcatctac gcttctccag
     6361 agattgatct cgagaccggg aatctcttct gataaattag aggagagcag ctcggctatc
     6421 ggaatcaacg ttgggcctac tggattgatg taattcaccc caaaagaaaa gagagtgatg
     6481 aaaagcagag ttgacactgc aacgaatttc ctcattaaga accacctcca tacccaatta
     6541 tacactgaat tagcgtctcc gacgacggct tatcagctat gaaggccaaa ttgagagccc
     6601 ggagccgaat ttgcgatctc agtgtgcttt tttctcccgc aaaggccaag atcgccctct
     6661 atgaggagtt tggtaatttg agctctgagt ggtagaataa tagcgacatc tcatgcgaag
     6721 gggggaacag aatgaacaaa aaagaactca tcgctgaaat cgctgagaaa acaggtgtta
     6781 ctaagaagga tgctggaaag acccttgaca cggtgatcga catcatcgag aagactcttt
     6841 caaagggtga tgtcgtaaga cttgttggct ttggcacatt tatggtggcg tcaaggaagg
     6901 ccagaaaggg tgtcaatccg agaaccaaga agcccatcac tataccgggc ggaaaagttc
     6961 cgaaatttgt acctggaaag gaactgaagg aaaaggtaaa atagtgtcgt ggactctgcg
     7021 gcggccggag ttgccgccgc ttttgttttg gagtgaagga tttgaaagga ttatggtttt
     7081 ttcttattgt cttgatcatt gttgtggcct tgggttcttt ctttaatatc tattttctga
     7141 agcagttcgg cgatgaaatg atttctagcc taagcacgag agagatcgag agcaaacttc
     7201 agctgatgtc tgaaagagtc tccctcctta acacgaattt agattctctt tcctctgttg
     7261 aagtgaggga ggatctttcg agaatcatta cggacttcga aagaataatc gcagatatta
     7321 actatgtctc gaactccttg agcagtaatc ccgtgcttga ttccatcgca cagctttctt
     7381 cagatttgaa gctgataaag agatccatcg actcactgga cagtggagaa agcgtagact
     7441 attcgactca aattgaaggg gtacggggaa agatcgtatc tctcgagtgg caggttgaag
     7501 agctgaagaa gctgctcgaa tctagcacag ctgatctgaa gaaatcggtc atgaatgtta
     7561 acgcgttttc aagtcaggct gatcagtctt ataaagagga agtcggaaaa ggcgttcgca
     7621 taagaataga atcgggcttc aaggggagca gcattcttca tgacagcgat atcctgatga
     7681 tcttgaacga gttatacgct ctgagggcca cggagatctc gctcaacggt aaaaggataa
     7741 tgccatacac atatgtacgc tgtgttggtg caactgttat aattaatgat gaaccgactc
     7801 aaatttcgcc cattgtgatt gaggttttgg gcgagtatga ttatcttgtt tccggtctgg
     7861 gtctcttgaa ggagtttttt gcaggcaggg agattgacat gacttttctg cctcttgaat
     7921 ttatcactat tcccgcggga gggggttgat tcagttgaga aaaagtgccc ttctatttct
     7981 tctggtactt atcatggttt tcttcacggg gtgtgacttc ccgtttatta cgagggatga
     8041 ttttacccaa agaatggatg cgctcgaaca aaaaatcgat tctctggcag ctggccagaa
     8101 gaagcttgcc cagctagagg atgccgtcga ggagcttgct ttcaggataa atttcgttca
     8161 ggcaattgaa cctacatatg caggttatga agaacttgag gctgttaggg cggagcttca
     8221 gataatcaag aacatgcttg ccgagtcggt tatagattca gcatcttccg cagcgatcgt
     8281 taagacactc tacgacaaga tcgacggagt tctaaccagt actgtggaaa acaacataat
     8341 tactcgggaa atcgatgact tgaacagaaa ggtacaggcg cttgaatcac taaactcttc
     8401 gaggtactcg gagttggttc agagaatctc cgagattggc acttacgata gttccattga
     8461 cgaggcgaag tatgaagagc tgctccagag gatcgaggag ataaagagcg cagaagttgt
     8521 cgttgtacag caggaatccg atgaagatga tcgcaggtac gaagaactcg ttaacaaggt
     8581 tgaggagctc gccgccagaa ccgatatctc cgttcttgac agcgctaagt atgattcctt
     8641 ggcaagaaga cttgccgagc tggagcagac cgtaggttca tctgtgatag attctgaggt
     8701 cttcaacgaa ctcgtttcta gaatagacga aatggattcc agaacgcgcg aactcgccga
     8761 taatctccag accattgagc ggcaggagga gcctgaagag aagaactttg tgacgatggc
     8821 cactttcctg ggagagattt ccgatataag aagcaggctc ggtgcagccg catacgagac
     8881 aattgaaccg acttcccatg tatcttacat tgttaaatcg ggcgataacc tgtggagcat
     8941 tgctcaagcg tatggggtaa ctgtggaaca gcttaaggcg atgaatcctg aaatcaagaa
     9001 ctgggatctg atctatcgtg gcgatgagat aaagatcccg ctttctctgg acaatctcat
     9061 ggccaaggct tccattgcca ctcattttgg actgaatctt ggcgtggatt ttcttgtgga
     9121 ttcgatagaa tctaatttcg gaagctatga ctatggttat gcaaatcccg gtatggacct
     9181 taccgtgcct ccaggctcaa gaatcacggc ttttcttccg ggaaaggtca tactttcgga
     9241 aagagtaaat gatttgtatg gcgagatggt tgtggtggac cacggaaaca acatgaagac
     9301 cgtatacgcg cgtctgggca gccgcatggt tatgaagggt gattttgtta gggttgggga
     9361 tactatcgga tcggcctcgg acgccaaagg aaatcttcat ttcgagttct ggaaagccga
     9421 tgtgcctgtc aatccagccg atataatatt cgagaatgtc ggaactttcg aagtgactat
     9481 gtacaccgaa tgggatgatg caaagaatcc tacgtcgcca tcattcaaga tgactgcgag
     9541 cggcgatttc gttaagggat atagaacggt agcagcagat ccagttgtaa ttcctcttgg
     9601 ttcaattgtc tatattccgt tcttctcatc ttctccaaac aagggtttct ttgttgtgga
     9661 ggataccggg agctcgatca aaggaaataa gatcgatgta tatacccatg acttcgatac
     9721 ggcatcgaat ttcaaagagg atcttcttgt gtacgtggtg aagaagcctt ga
//
LOCUS       DAOQYI010000054         9383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_7817_length_9383_cov_79.006539, whole genome shotgun sequence.
ACCESSION   DAOQYI010000054 DAOQYI010000000
VERSION     DAOQYI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7817_length_9383_cov_79.006539"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1483
                     /locus_tag="PKL93_09610"
     CDS             77..1483
                     /locus_tag="PKL93_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ71553.1"
                     /translation="MNYGRLNAGLIEELQALLKIPVKYDEESLDRYSRDETAELRAVK
                     PEVVTFPVSTQEVSEIMKFANRHLIPVTPRGAGTGLSGGAVPSCRGIVMSFEKMNRIL
                     EFDEANMMITVEPGVITGEIQKLADRHNLVYGGDPCSSESSSIGGNVAENAGGNKVMK
                     YGPTGYHVYGLEVVLPSGEVTFFGGKRLKDVSGLDFVHLMVGSEGTLGIVTKITLRLL
                     PKPKYSVALLVPYPDIDTAIAAVPGLMSGSGVVPTSLEFMDGSSIRATCSFLNIEFPY
                     ADAGAHLIIEVEGNSKDSIFDDYVKLGEAAIEAGGLEAFVADNRNTRDKLWKARKSIA
                     EALAAISPVHSMEDVSVPMANIPKLIKRSEEIAKKRGLEMIAFGHAGDGNVHVTFIKG
                     DLTQEEWNRNLPLAEKELFDETTELGGCISGEHGIGIKRKKYLASIVDEAQLDLIRGI
                     KMAFDRNNILNPGKIVDL"
     gene            2625..3254
                     /locus_tag="PKL93_09615"
     CDS             2625..3254
                     /locus_tag="PKL93_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNQ71554.1"
                     /translation="MDLRKELVLFARLAWDRRLTESTGGNMSVRNGDHFVITPTTMVK
                     HFLTEEDLVEVDLNGRKVSGRREPSSEYRMHLKIYRECEDVVSVFHAHPVYATTIAVQ
                     QKKFPVNVLPETALMLAPITYLPYRMPGTDDFAEIFDEGLKLGSRTFVLRNHGVTVGG
                     SSIEEAYAKLETLEFLAQITLLSGAKPGYLEIPSEDVEKFLKKIRGSKE"
     gene            complement(3642..6140)
                     /locus_tag="PKL93_09620"
     CDS             complement(3642..6140)
                     /locus_tag="PKL93_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71555.1"
                     /translation="MKKLTYVLLVMLLLLGGLALSSQKGPYPDLVYFNVRMSEEIALK
                     DTAEGLTDIFMWGLSGPQIFGLDQATRDKLDLYAVPSGSWSLLMNPIPNKAPYVVEVG
                     GKEYFNAFAIRDIRFAMNDLINRQYLVDEILGGAGTPAFTMATTGQPGTYRYNLLATK
                     FGLTAEGNEARALEVIESSMQAAAALPENVGRLEKKDDGFWYFDNEPVTIKIAIRVDD
                     PQGRLKEGDYIADQLEKAGLKAERLYWDRIKCSNVVYGGDPAEFTWQMYTEGWGAGAT
                     RAFWEHIVAQMYAPWYGYMPGGMTEGFWNYEQDEIDEVTLKAYTGNFLTEEEYWELAL
                     RGLELGLEEAVRIYVVNQLDFFVANASRLKQRFAYGLGDGLNTWSIRTAVTDDRTLFI
                     TQFSAMGSLFMDAWDPIGTDGFDSVYSNYIAENLYDPAMFESPASAIPTPLRAVPIEV
                     QTKARRNEEGDVVGDYEVPANAINYCPFNEAWLQVGEGKKSISMATYEFKFGKLHHGQ
                     PISVVDLLYAQAFQMEWASKEGDDDLQYEQAYSSSLKSGLDTIIGWVLNEDDTITVYF
                     DYNFPASKERVASWAAPGISVSSSGHPVGCAWEIAEAMSLLVTEGSESGTAYSITMDP
                     AFTEVDAIAPACVKDIRAKLVEMKERKHIPNYIKEFVDEEYVLGRYEAAIEFIDNYGH
                     AYISNGPFYLSKFDSTSNYMELRAFRDPDYPYEDTYWIEELKAVRMQIDSLETPAFVA
                     KGSDLPVSVYVSEVTYPIDEAVPATTGKVTLSLIVGQEAREFEAAMTEAGLFECVIPA
                     SAIEDLGAGSYDIMAIAELEGAIPASASTSIVIY"
     gene            complement(6675..7844)
                     /locus_tag="PKL93_09625"
     CDS             complement(6675..7844)
                     /locus_tag="PKL93_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNQ71556.1"
                     /translation="MSETVHRIMLDDKELIIIGTAHVSKNSAEEVKAIIEEEKPDSVA
                     IELCNSRYQSIQDQDKWKKTDIAKVVKEKKSFLLLANLILSSYQKRMAKQIGIQAGQE
                     MLQAIESAKETGAELVLADRDIQVTFARIWGNLGFWGKTKLFFTLVLSIFSDEKITEE
                     DLEKMKSGDMLTSALSELAKSFPQLKESLIDERDKYLAQKIKTAPGKKVVAVVGAGHV
                     PGLKEAIKEDQDLVALTKIPPKKKTGKIIGWTISIAIIALIVSTLIVNRDAGFDQILS
                     WILWNGSLSALGTLIAFGHPLSILTAFFVAPVSSLNPLLAAGWFAGLVEAIVRKPKVS
                     DFENLNEDVNSFKGFWRNRVTKILLIVVFANVGSSVGTFIGGAEVVRIFINSFFG"
     gene            7962..8921
                     /locus_tag="PKL93_09630"
     CDS             7962..8921
                     /locus_tag="PKL93_09630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71557.1"
                     /translation="MSNPARAEIEDLQGMVDLASLVFKSEMGKIFPVLFSQENVENMT
                     IVKEEGRPVSMIGLLPREISFFGHRMKVGLIGSVCTHPDYRGKNYGAVTLAKAEEHAI
                     DMGLSLLIISGGRGLYQRFGAVRPPGTKRILVKPGRTASMREAKISDIGKILDLYRMK
                     PLRYIRNFTDFEKTFSTGYAEARKAKAYLSEKAYITVVEREDNHYVIEYGGGSKDVIS
                     LTRGYIEKLGLKGCIIVAERHFKAEESLPIEFPGTAKIISKRSFFDQMEGYFTEIMPS
                     EDYDRFVETVERLSLAEFNQEVFCCSKFKGLPLSLPNYGFDYI"
BASE COUNT         2479 a         2220 c         2259 g         2425 t
ORIGIN      
        1 cagatattgg gcttgttgga gacgctgtcg agattctgaa cgaacttgca aagtcccttg
       61 agtcggaggt gaagagatga attacggacg tcttaacgcg ggcctgatag aagagcttca
      121 agcactactc aagatcccag tcaaatacga tgaggagtca ctggatagat attctagaga
      181 cgagacggcc gaactgagag ccgtaaaacc ggaggttgtc acattccctg taagtactca
      241 ggaagtgtcg gagatcatga aattcgcaaa caggcatttg attccagtta ccccaagagg
      301 tgcgggaacg ggcctttcgg gaggcgcggt gccttcatgc cgtggaatcg tcatgagctt
      361 cgagaagatg aacagaattc tcgagttcga cgaagcaaat atgatgatta ccgtcgaacc
      421 gggagtgata accggcgaaa ttcagaaact ggcagacagg cataacctag tttacggagg
      481 tgatccctgc agcagcgaga gctcctcgat cgggggcaac gttgcggaaa acgccggagg
      541 aaacaaagtc atgaaatatg gaccgacagg ttaccatgtt tacggccttg aggtagtcct
      601 tccctcaggc gaggtcacat tctttggagg aaaaagactg aaggatgtca gcggcctgga
      661 cttcgtccat ctcatggtcg gatctgaagg cacgcttgga attgtcacga aaataacgtt
      721 gaggctgctg ccaaagccaa agtattcagt ggccttgctc gtaccatacc cggatatcga
      781 tacggcaatt gcagcagtac ctggattgat gagcggttca ggtgttgtcc ctacttctct
      841 ggagtttatg gacggctcgt cgataagggc gacctgcagc ttcctgaaca tcgagtttcc
      901 gtacgctgac gccggagctc atctgataat agaagtcgaa ggcaacagca aggattcgat
      961 attcgacgat tacgtcaaac tcggagaagc cgctatcgaa gcgggcgggc ttgaagcctt
     1021 tgtggcagac aaccggaata cgagagacaa actatggaag gccagaaagt cgattgcgga
     1081 ggcactggcc gcaataagcc cggtgcacag catggaagac gtctccgttc cgatggcgaa
     1141 tatacctaag ctaataaaga ggtctgaaga gatcgcaaag aagcgcggac tcgaaatgat
     1201 tgcctttggc cacgcaggag acggaaatgt ccatgtcact tttataaagg gagatcttac
     1261 tcaggaagag tggaacagaa atctcccgct ggccgaaaaa gagctctttg atgaaaccac
     1321 agagttaggg ggctgcataa gcggagaaca cggtataggt ataaagagga agaaatacct
     1381 cgcatcaata gtagacgaag ctcaacttga tctaattcga ggcataaaaa tggcctttga
     1441 tcgaaacaac atactcaatc cggggaagat agtggatctt tgaaaaaccg gggtcggggg
     1501 tagcgggctg ggggtcggaa gagcaaaaaa accagttgca agttccaagt tgtgagttgt
     1561 aagaccgagg tcggaggtcg gaagatcaag agaaactggt tgaacgttga gcggttctcc
     1621 gttcgccggg agagccgtga agcaattcat gaagattccg accaggagct ttgtcggaat
     1681 gacggaattg ggcgttttcg gaataaccgt tcttggtcaa accattattc agagagtaga
     1741 gagaatggaa aagcaaagag ggcaggtaag gttccgcgat attgaaaagc tcttaatccc
     1801 ctctcgagag gggtgtcgac ggctccatct tcgacgtggt gtgtgcacat caagaactga
     1861 cctcattttc agtcatgacg gaattcttta ccaagaacga acaacctgga cgcgtggcgt
     1921 cggaacgaag aaccggtctt ttctcggcgg aggacgcagg accgttaatg ggcaacgcgg
     1981 ttttcatcag cgaccagcgg gttcttgctc ttaagcatac agcgaatctt tggatcgaga
     2041 tgctgaaaca agttcagaat gacggaatta gtagtggatc tcgggtctcg attctggggt
     2101 ttcgcgaaca gacccggctc atcaattcag ggttttaccg taactgagaa agttatccgg
     2161 gcagcgggtt tgggaagtcc ctcaaattgt catcccggac ttgatccggg atctggttca
     2221 aaaaacgaac aacgaagaac cggttttctt ggcggaggac cgttgacggg caacatggtt
     2281 ttcatcatcg tacaggggtc ctgcaaagga tctcgctccc aaacctctct ttctttgttt
     2341 cttgcctcct cgcgtgagcg ccagcgtcac ttcctgagta acagcctcac ttccccggac
     2401 gcttttccgg gcatcacttc atgggatgtt cttcccagca tcacttcttt agaggcttgc
     2461 cgtcattgcg tcatctgatg ccccttagtg caatgcatcc cgccgaaggg gagactgagg
     2521 caaagcctga tggtcacgtg tagcgtgact ggctataata gaatcgactt atagttattt
     2581 catgctatat atggagcaaa atatagaaca aaatggaggt cactatggat ttgagaaaag
     2641 agctggtcct tttcgcgagg ctggcatggg acagaagact taccgagagt accggtggaa
     2701 atatgagcgt tagaaatgga gatcattttg tgattacccc cacaacaatg gtgaagcact
     2761 ttctcacgga agaggatctt gtcgaggtag atctgaatgg aaggaaagtt agtgggaggc
     2821 gcgaaccatc atcggaatac aggatgcatt tgaagattta cagagagtgt gaagacgtgg
     2881 tttccgtttt ccatgctcat cccgtttacg cgaccactat cgcagttcag cagaagaagt
     2941 ttcctgtgaa tgttcttcct gaaactgcgc tcatgctggc tccgataacc tatcttccct
     3001 acagaatgcc tggtacggat gattttgcgg agatattcga cgagggtttg aagctgggct
     3061 cgcgaacctt cgttttgaga aaccacggtg ttacagtcgg cggaagtagt attgaagagg
     3121 catatgcaaa gctcgagacg ctggagtttc tggctcagat caccctcctt tccggtgcca
     3181 aacccggtta cctcgaaatc ccttccgaag atgtggagaa gttcttgaaa aaaataagag
     3241 gatctaagga gtagtttcag ccattcttta gattttcacg cagaagcacc cgagattgcc
     3301 tataaagtcg cgtttcaggg tgaatttatt ctctccagtt gcttttcaaa ggaaggagtt
     3361 ttgtctcttt actcgaaaaa tatacttgtc aaaagagttt atccactgga aaccgctgct
     3421 gctgccttca attagacttc acgattgttt ttggttcgat tctcttttga ctgaattact
     3481 gatatttcag aaggctttat tcagtttgaa aaaaacagac tgtgcacgag aagtccatct
     3541 ggtgggtttg tagacttcca ctccatcttt agtcgctgta caggacaaat gaaagaagag
     3601 gcagtgtcgt agtagacact gcctcttcaa cttgaacctt atcagtagat tactatagaa
     3661 gtcgaagcag aagcgggaat ggccccttct aactcggcaa ttgccatgat gtcgtacgat
     3721 cctgcgccga gatcctcaat agcactcgcc ggtatcacgc actcgaacaa tccggcttcg
     3781 gtcattgcgg cttcgaactc ccttgcctcc tgtccaacta tcagggacag agtcaccttt
     3841 ccggttgttg cgggaacagc ctcatctatt ggataagtga cttccgagac gtaaacactt
     3901 acaggaagat cgctgccctt agccacaaag gcaggcgttt ccaagctgtc tatctgcatc
     3961 cttacagctt tcagttcctc tatccagtat gtgtcttcgt aagggtagtc gggatctctg
     4021 aaagctctca attccatgta gttggaagtc gagtcgaatt tcgacagata gaaaggtcca
     4081 ttgcttatat aggcgtggcc gtaattgtct atgaattcga ttgcagcctc gtatctgcca
     4141 agtacgtatt cctcgtcgac aaattctttg atgtagttcg gaatatgctt tctttccttc
     4201 atctcgacaa gtttagctcg gatgtctttc acacaagcag gagcaatggc atctacttct
     4261 gtgaaggcgg gatccattgt aattgagtaa gcagtaccgg attcgctgcc ttcagttaca
     4321 aggagagaca tggcttctgc tatctcccag gcacagccaa ctggatgacc cgacgaagaa
     4381 acgcttatac ctggtgctgc ccagctagca actctttcct tgcttgccgg gaagttgtaa
     4441 tcgaagtata cggttatggt gtcgtcctcg ttcaaaaccc aacctatgat tgtatcgaga
     4501 ccactcttaa gagaagaaga atatgcctgt tcgtactgga gatcatcgtc accctctttg
     4561 ctcgcccatt ccatctggaa tgcttgggca tagaggaggt caacgaccga aatcggctgc
     4621 ccatggtgaa gtttgccaaa cttgaattca taggtagcca tggagatcga cttctttcct
     4681 tctccaactt gtagccaggc ttcgttaaac gggcagtagt tgatagcatt tgcgggcact
     4741 tcgtaatctc cgacaacgtc tccttcttca ttacgtcgcg cctttgtttg aacttcaatc
     4801 ggtaccgctc tcaaaggtgt tggaatagcc gaagctgggc tttcgaacat tgccgggtca
     4861 tataggtttt cggcaatgta gttcgaataa acactgtcaa aaccatccgt ccctatcggg
     4921 tcccaggcat ccatgaagag agatcccata gccgagaact gtgttatgaa cagcgttcgg
     4981 tcatctgtaa cggccgttct tatcgaccat gtgtttagac cgtctcccag cccatatgcg
     5041 aacctctgct tgagtcttga tgcgttcgcg acaaaaaagt cgagctgatt gacaacgtat
     5101 atcctaacag cttcttccaa accgagttca aggcctctta gcgcaagttc ccagtactct
     5161 tcctcagtca agaagttacc ggtataagcc ttgagagtga cttcatcgat ctcatcctgt
     5221 tcgtagttcc agaagccttc tgtcattccg cctggcatgt atccatacca gggagcatac
     5281 atctgcgcga cgatatgttc ccagaaggcc ctcgttgcac cggctcccca gccttccgtg
     5341 tacatctgcc aggtaaactc cgccggatcg cctccgtaga cgacgttact gcacttaatc
     5401 ctgtcccagt acaacctttc cgccttcagt cctgcctttt caagctgatc cgcaatgtag
     5461 tctccttcct ttagacgacc ctggggatca tcgaccctaa tggctatctt gattgttacc
     5521 ggctcattat cgaagtacca gaatccgtcg tccttcttct ccagcctgcc aacattttcc
     5581 ggtagtgcgg ctgcagcttg cattgagctc tctatgactt ccaaagccct tgcttcgttt
     5641 ccttcagccg tcaaaccaaa cttcgtggcg agaaggttgt acctgtacgt tcccggctgg
     5701 ccagtagtag ccattgtgaa ggccggtgtc ccggctcctc cgagaatctc gtcaacaagg
     5761 tattgcctgt tgatcaggtc gttcatcgcg aatctgatat ccctgatcgc aaaggcgttg
     5821 aagtactcct tccctccaac ttcaacgaca tatggcgctt tgttagggat cggattcatc
     5881 agtagcgacc aggatcctga tggaacagca tacaggtcaa gcttgtctct agtggcctga
     5941 tccagcccga agatctgtgg ccctgaaaga ccccacatga atatatctgt aagaccttca
     6001 gccgtgtcct tcagcgcgat ctcttccgac attctcacgt tgaagtaaac aagatcagga
     6061 taaggtccct tctgcgagga cagagcaagt ccgcccagca gcaatagcat gaccaacagc
     6121 acataagtta gcttcttcac ttccttcgcc tccttaagaa attagcgttt cgatcgagtc
     6181 acaacttgaa ctgggataaa gttgtttcgt ctacgtagtg gcaagaaacg agagagataa
     6241 atatagaacg aaaaatcagt gtgtttgatt gtcgatgctg aagtaatcca tgcttcacga
     6301 agaagctaat gtatttgcaa gattattata ttacattcac atgcaaaatc agaagaacaa
     6361 ataggcccaa tttacggtgt ctttgccaga taaaaccagc cctgttactt tgtctttcta
     6421 taactcctta ttgtgcgaga atacttcatg aaatagcttt tcgataacgc tgagaagttc
     6481 attttccagc tttagaacat tacataaata cttattattc ctttttcaat gatttgataa
     6541 tttcttgtta tccagattct tggtctcaag acctgaaata ccaatctatc cgacaatatt
     6601 gaccaaactc aaataattag aattcctcac cttgattcaa ttaattagta cgatatgata
     6661 gcactttcgt gctactaccc gaagaaagaa ttgatgaaaa ttctaactac ttcggctccc
     6721 ccaatgaaag tgcctacact gcttccaacg ttcgcgaata ccacaatcag aaggattttc
     6781 gtgactctgt ttctccaaaa acccttgaag gagtttacat cttcgttcag gttctcaaaa
     6841 tccgagacct tgggctttct tacgatcgct tccacaagtc cggcaaacca tcccgcagcc
     6901 agaagcggat tcaatgagct tacaggggca acaaagaacg ccgtcagtat agagagaggg
     6961 tgaccaaaag ctataagagt tcctagagca gaaagcgatc cattccatag gatccagctt
     7021 agaatctgat caaaccctgc atcgcgattc actattaacg ttgaaacaat caaggcgatg
     7081 atcgctatcg agatcgtcca gccgattatc ttaccggtct tcttcttcgg agggatcttc
     7141 gtgagtgcaa caagatcctg atcctctttt atggcctctt tcaaacccgg gacatgcccg
     7201 gcaccaacta cggcgacgac tttctttccc ggagccgtct tgatcttctg ggcgagatac
     7261 ttgtcccttt cgtcaatcaa tgactccttg agctgaggaa aggacttggc aagctcactg
     7321 agcgcagagg tcagcatatc ccccgacttc attttctcga gatcttcttc tgttatcttc
     7381 tcatcgctga agatagacaa aactagagtg aagaagagtt tcgtctttcc ccagaagcca
     7441 agatttcccc atattcttgc gaaagtgacc tgaatatctc tatctgcaag aacaagctcc
     7501 gctcctgtct cttttgccga ctcaatagcc tgcagcatct cctgtcctgc ctgtattcct
     7561 atttgttttg ccattctctt ttgataagag gaaagtataa ggttagctag cagaagaaaa
     7621 gacttcttct ctttaactac ctttgcgata tcggtcttct tccatttatc ctgatcctgt
     7681 atcgattggt atcgggagtt gcacagttct attgcaacgg aatcgggttt ttcctcttcg
     7741 ataatcgcct ttacttcttc ggcgctattc tttgatacgt gagcggtacc gataattatc
     7801 aattctttgt catcaagcat tattctgtgt actgtctcgg acaacgaaat caccctttcg
     7861 atttgactat caaattctat ttaatcacaa tcttgcaccc aactcttctt tgtgagatta
     7921 tcaattatat agctcattag atattcatgg gaggtgttct cttgagcaat cccgcacgag
     7981 cagaaatcga agacctacaa gggatggttg accttgcatc tcttgtcttc aaaagtgaaa
     8041 tgggaaagat ctttcctgtg ctgttttcgc aagagaatgt ggaaaacatg acaatcgtta
     8101 aggaggaggg aagaccggtg tcgatgatcg gcttactccc tagggaaatc tctttcttcg
     8161 gacacaggat gaaggtcggt ctcatcggtt cagtatgcac tcacccggac taccgtggga
     8221 agaactacgg agccgtgact cttgcgaagg ccgaagaaca tgctatagat atgggcctgt
     8281 cactactcat aatttccgga ggcagaggcc tataccagcg gttcggagcg gtcaggccgc
     8341 caggcacgaa aagaattctc gtgaagccag gaagaacggc atctatgagg gaagcaaaaa
     8401 tttcggacat tggtaagatt ctcgatcttt acagaatgaa accactgaga tacataagga
     8461 acttcacaga tttcgagaaa accttctcaa cgggttacgc ggaggcacgt aaggcaaaag
     8521 cgtacttgag tgaaaaggct tacattaccg ttgtcgagag agaagacaat cattacgtga
     8581 ttgaatacgg cggaggcagc aaagacgtaa tctcattgac aaggggctat atcgaaaaac
     8641 tcggactcaa gggatgcata atcgtcgccg aaagacactt caaggcagag gagagccttc
     8701 cgatcgagtt tcccggcacc gcgaaaatca tctccaagag aagcttcttc gaccaaatgg
     8761 agggttactt cacggagatt atgccttcag aggactatga cagattcgta gagaccgttg
     8821 aaagactgtc attggcggaa ttcaaccagg aagtcttttg ttgcagcaaa ttcaaaggac
     8881 tgcctctttc acttccaaac tacggatttg attacatttg aaaaccggcg gaggcgaggc
     8941 cgactacgtc gggaagtgag gcttgctggc gcgaggaagt aatgctggga aaagcgtccg
     9001 gggaagtgag gctgctacgc agaaagtgat gcaagaaaca aagaaaaaga ggtttgggag
     9061 cgagatcctt tgcagaaccc ctgtacgctt atgaaaaacc gttttccgtc aatggttctc
     9121 cgtccccgcc aagaagcatc ggttcttcgt tccgacgctg cgcgtccagg ttcttcgttc
     9181 ttggaacaga tcctggatta agttcgggat gaccctgaac agagacccca aacaggggca
     9241 ttttggggca ggcttcaggg caggctcagt gggtggacaa ctgacggtta ttccgaaaac
     9301 gcccaattcc gtcattccga caaagctcct ggtcggaatc tctatagtat tgcttcatgg
     9361 ctctcctcgg cgaacggaga acc
//
LOCUS       DAOQYI010000055         9170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_8057_length_9170_cov_79.763467, whole genome shotgun sequence.
ACCESSION   DAOQYI010000055 DAOQYI010000000
VERSION     DAOQYI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_9170_cov_79.763467"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKL93_09635"
     CDS             complement(<1..655)
                     /locus_tag="PKL93_09635"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNQ71558.1"
                     /translation="MALGVDRITNTVYVESNSFDPWLNLAIEEYLLNSYARDSSVLYL
                     WQNEKTVVIGRNQNAWQECRLDNLERDGGRLARRLSGGGAVYHDLGNLNFTFLMPRKE
                     YNLHRQLSVIVDAVKELGIEAHFSGRNDILAGGKKFSGNAFYHGQFASYHHGTILIDV
                     DTGKLSEYLNVSKEKIESKGVKSVVSRVVNLKEMKPDLTIDTMKKAMYRGFIEEYGQI
                     "
     gene            complement(636..1475)
                     /locus_tag="PKL93_09640"
     CDS             complement(636..1475)
                     /locus_tag="PKL93_09640"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ71559.1"
                     /translation="MKAKFALILYVLFIFANACGVGSIRYLVRPGDRLFNIIRDYNLR
                     ISTVLDLNCIANPRQLEPGEFINLPTGDGFFYEVQYGDSIDFIAKLFFALAEEIMAAN
                     GLKCTSVIYPGQRLFIPLESINVCGSINPGTQFTWPVYGKISSEFGWRKDPFTGVSTF
                     HSGIDIAVQKDAPVFASKDGMVMEAQESGGYGLNIIIQHYDGSKTRYSHLNHISVYVG
                     QRVLRGELIGRVGETGRATGPHLHFEIIDSNDQCRNPRSYLTGINYMYVRREADSLGV
                     GGR"
     gene            complement(1693..2013)
                     /locus_tag="PKL93_09645"
     CDS             complement(1693..2013)
                     /locus_tag="PKL93_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71560.1"
                     /translation="MFEKIDYWVSAASYSDRNGTWLLEAALIHPNIGESHDYGEEWTR
                     EEIIDKCDVFVFCLITKDAKGNWKRGGQLRKIETDKGVFIRVDGLEKTGDYLGDIPFI
                     EEIE"
     gene            2103..3968
                     /locus_tag="PKL93_09650"
     CDS             2103..3968
                     /locus_tag="PKL93_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNQ71561.1"
                     /translation="MTFVNDTSRSPRAQVRPLAIEKVELQGFVGRYQGLMKSTSLPMQ
                     YEYLESTGRIDNFRKAAGNMEGSFTGWFFNDSDVYKWIEAASYSLSYNEDPEIRARIE
                     SLITLIEKAQEISGDGYINTYFVGQKAAERWKDLKNMHELYCAGHLIQAGIANKRASG
                     DETLFKVCVSAADNILDSFRDDDCKVTTGHPELEMAMIELHRETGNRDYLKFAQMLID
                     NRGRGYVGGDEYHIDHVPFRELKELTGHAVRMLYLLAGAADIFLETGDETLLAVLERL
                     WIDLTARKMYVTGGAGSRYEGESFGEEFELPSRRAYAETCAAVGNVFWNWRMYMISGD
                     AKYLDLFEQSFYNGVLSGISLDGKRYFYVNPLEDAGKREREEWFECACCPPNIARLLT
                     SFGGYIYGTTLNEIRVNFYEESKATIPFRDGEVSIIQKTSYPHSEEVQLTVATDLDTE
                     LSILLRIPEWTEGEFEVQVDGIKQKLRPEKGFVRLEGNWKGKTEVYLALPMRIRLMTA
                     NPLLRENTDKVSVQRGPLVYCAEGVDNPDFDVRTLSVPSRKNFEVTQSDALPGNPVIL
                     SGKGVAFDLDGWEKKLYNPLGSVKSKGNNVRFSLVPYYAWNNRGKSTMCVWLHVH"
     gene            4102..5985
                     /locus_tag="PKL93_09655"
     CDS             4102..5985
                     /locus_tag="PKL93_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ71562.1"
                     /translation="MSYLRTFFVIIILAFSASCLAYEGQILFSGNEIHYEVEEADGEV
                     VILIGDGPGFSSSYMKGILSGRKTLRYDQLESGSSKNHSDVAIDFQYFVAELASLIFS
                     LEINSFHLLGHGFGSAIAVGFALTNPEGLLSLVMINPRLNLPAIDSALSDLADGESSQ
                     SFDKFHYLFGDGPTSELKPVLSRDIINEEIYNLFWGETPTFISGILRGHDFSSGLLDI
                     NVPILICSGLNSFPGVKNLLTFQEGSPNIEFVTFMNSGHLPMLEEKKAFKTIVEDFLQ
                     RAEENFSHQGQQEMNLDDMSEVPGDMVFGWEDLEKVHELHVGDDFSVKLPSNRGAGYF
                     WKVKSLDENSIRLISDPYYEELKESGGGRDVFDFRVVGSGKSSIEFSFGSLWEETSIR
                     TSSFSINVEKPNIDPIIIDSSDVGKTIVVGLNEPIEVILDSPIDSGLRWRISATTPEV
                     LRQPRENEVKITEPSPYSAGKVAQTFYFEGMNYGQARLRFDYGSLWEDVAPEKTFEAT
                     IVVAEPVREYVFVQDSDYGRTISIRIDQTVLVRLKKGNEEDCEWQLSSYSAVEPVGDI
                     TEVEYMGVRYSVFRLKPTDSGDSMLEFLYFEGEKRREQVDAFKIGLVITEESGQEMSP
                     DQK"
     gene            complement(6059..6643)
                     /locus_tag="PKL93_09660"
     CDS             complement(6059..6643)
                     /locus_tag="PKL93_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HNQ71563.1"
                     /translation="MQLSSFGEGTIKHFTWLNEPKRWELQDGNLIVESEESTDFWQRT
                     HYGFRNDNGHFLFVPLMEDFSFVLGAKFFPVNQYDQAGVMVRVSPSCWLKSSIEFEGS
                     APSKLGAVVTNSGYSDWSTQDVSCETRSLRVKVDRKGSDYSVFAWQDEWVQLRLARLI
                     EDDCKLPVMVGAYCCSPRGSGYRVVFDEISLNCR"
     gene            7002..8042
                     /locus_tag="PKL93_09665"
     CDS             7002..8042
                     /locus_tag="PKL93_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71564.1"
                     /translation="MYWKYAIKRVLYGTLMYAILVFIFSALFNTVMEQTLRAQIEEQI
                     RGETMRMTSLNESQLEDYVINRRNDLYSLYRLTRPVAERILWRTWDTLTLNLGNSTII
                     RSSNGSRNVWDVVSEAIPKTILLFSVAMLVDIVLGLLLGLKKAQKAGGLLDKSTSVGT
                     MVVFGMPSWWLGMIMIMFFAYGVKIFPSGGLHSTPPPEGISYFFDLLYHLALPVLTLV
                     VIGFWGRAFLTRNIVLGVLQEDYIMAARARGIPERKVLYGHTMRTSAPPIVTMSLLAL
                     LASVGGNIVYEGIFSWPGMGNLYWIALQQNDVPVLMGNLAITTGLYICGLVLLDLIYG
                     LLDPRIKVGGKQ"
     gene            8045..>9170
                     /locus_tag="PKL93_09670"
     CDS             8045..>9170
                     /locus_tag="PKL93_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71565.1"
                     /translation="MSDLRRSASDFWKEFRKVRSGMIGLIFLVVFICILIFEPIIVPY
                     PEVNEKWRDITYWQDLPSSAPPSWINLFTSKKRAVSETIEKYTFSETLAGAMRIQEYV
                     FEYDYSASVAPSDVVFHASGVGKPTVVISIERPDGLSVDLYRKQLETSPSQDARVSID
                     RSAQTSAMNFGKKYSPSEGVPLQTIKTTDVLFSKAQEGIFRNPEPLVGTYRLKVTLLL
                     QKPDERIDNVFISIAGRVHGILGTDNSKRDIWSGVIAGVKWAMLIGLLTALVSVSIGV
                     VYGVISAYVGGWKDSLMQRIFEIFISVPMLPVLIVMSAVFKPNIWVMIGIMCIFYWVG
                     PVKTVRSMGLQIKEETYIEASRALGASNTRIIFKHMVPLLI"
BASE COUNT         2557 a         1996 c         2047 g         2570 t
ORIGIN      
        1 ctatctggcc gtactcctca atgaaacctc tatacattgc ctttttcatt gtgtcaatgg
       61 ttaaatccgg tttcatttcc ttgagattga caactctcga cacaactgat ttaactccct
      121 tggattcgat cttttccttt gagacattta gatattcgga aagctttccg gtgtctacgt
      181 ctatcaaaat tgttccatgg tgataggagg caaattgccc atgatagaaa gcatttcctg
      241 agaacttctt tccgcccgcc aaaatatcgt ttctaccact gaaatgtgct tcaattccta
      301 gttcctttac agcatctaca attacagaaa gttgcctgtg gaggttgtat tcctttctcg
      361 gcatgagaaa cgtaaagttg aggtttccaa gatcatgata gacagcccca ccgccagaca
      421 atcttcttgc cagtcttcca ccatcccttt caagattgtc gagtctgcat tcctgccatg
      481 cattctgatt tctgccgatt accaccgttt tttcgttctg ccacagatac agcacgctgc
      541 tatcgcgggc gtacgaattc aaaaggtact cttcaatggc aagattcaac cagggatcaa
      601 aagagtttga ttcaacgtat actgtgtttg taattctatc tacccccaac gccaaggcta
      661 tctgcttctc ttctaacgta catgtagttt atacctgtaa gataacttct tggattcctg
      721 cattgatcat tggaatcgat aatctcgaaa tgcagatgag gtcccgtagc tctcccggtt
      781 tctcccactc taccgattaa ctctcccctt agcactctct gaccaacata tacactgata
      841 tgattcaggt gtgaatatct tgttttgctg ccgtcataat gttggattat tatgttcagt
      901 ccatacccac cgctttcctg cgcctccatt accattccat ccttggaggc aaaaacgggc
      961 gcatcttttt gaactgcgat atctataccg gaatgaaagg tagaaactcc ggtaaaggga
     1021 tcctttcgcc aaccaaattc cgaagagatc tttccatata ccggccaggt aaactgcgta
     1081 ccaggattaa tacttccgca gacatttatt gattcaagcg ggataaacag cctttgtcca
     1141 ggataaatta ccgaggtaca tttcaaacca tttgcagcca tgatttcttc ggcaagagca
     1201 aagaaaagct tcgcgatgaa gtcaattgaa tcaccatact gaacttcata aaagaaaccg
     1261 tctccagtag gaagatttat gaattcccca ggttcaagct gacgaggatt ggcgatgcag
     1321 ttcaggtcga gtactgtact gattctcaga ttatagtctc tgatgatgtt gaacaatctg
     1381 tcgcccggcc taaccaggta tcttatagaa cctactccgc aagcgttcgc aaaaataaac
     1441 aaaacataaa gaatcaaagc aaacttggct ttcatgagaa cccccaaacc gtatttttgt
     1501 ccgcaactaa gaaatcaatt aatataagat gttgtaataa tcttaataat atcacagtgt
     1561 gtatcgtcag aagaagctgc aaaggaacct cttttatttg gagaaatatc gaccacttct
     1621 gaccaacata aaactgaaca tatttggatc ttcttaatgg ctcaagttag gatcagacct
     1681 aattcaagag agtcattcaa tctcctcaat aaaaggaatg tctccaagat aatcccccgt
     1741 tttttccaaa ccgtcgactc ttatgaaaac tcccttgtca gtttcaatct ttctaagttg
     1801 ccctcctctt ttccagttcc ccttcgcgtc ttttgttata agacaaaaca caaatacgtc
     1861 gcacttatcg attatttctt ctcgcgtcca ttcttcccca tagtcgtgag actctccaat
     1921 attcggatga atcagcgctg cctcgagtag ccatgtacca tttctatccg aatagctcgc
     1981 agctgaaacc caatagtcta ttttctcgaa catggcaaaa tacctcctta cctaaggata
     2041 caaaagtgtg accgttttct gctactcttg acaagagagg atcttctttg gaggtgagaa
     2101 cagtgacatt cgtgaatgac acgtcacggt caccaagagc gcaagtaagg cccctggcta
     2161 tcgagaaagt ggaactacag gggtttgtcg gaagatatca gggactcatg aagtctacca
     2221 gtttgcctat gcaatacgag tatctggagt caactggaag aatagacaac tttcgaaagg
     2281 cagcagggaa catggaaggg agcttcaccg gctggttttt caatgattcc gatgtttata
     2341 agtggataga ggcggcgtct tactctcttt catataacga ggaccccgaa atccgtgcca
     2401 gaattgagtc tttgataact cttatagaaa aggcacagga gataagcggt gacggttata
     2461 tcaacactta ctttgttggc cagaaggcgg cggaaagatg gaaagatttg aagaacatgc
     2521 acgaactcta ctgtgcgggc catcttatcc aggcaggtat agccaacaag agagcctctg
     2581 gtgacgagac gctgttcaag gtttgcgtaa gtgctgccga caatattctt gacagcttta
     2641 gagatgacga ctgcaaagtg accacaggcc atcccgagtt agagatggca atgatcgaac
     2701 tacacagaga aacaggcaat cgcgactatt tgaaatttgc tcagatgctt atcgacaatc
     2761 gcggtagagg ttatgttggc ggcgatgagt atcacataga tcatgtaccg ttcagagaac
     2821 taaaagagct taccggtcat gcggttagga tgctgtactt actggcgggc gcggcagaca
     2881 tatttcttga aactggagac gagaccctgc tggcagtcct tgaaagacta tggatcgatc
     2941 tcacagcaag gaagatgtac gttactggtg gcgccggctc aagatacgag ggagaatcct
     3001 tcggagaaga gttcgaatta cccagcagaa gagcttatgc ggaaacatgt gctgcagttg
     3061 gaaatgtctt ctggaactgg cgcatgtata tgatatctgg tgatgccaag tacctggacc
     3121 tattcgagca gtctttctat aatggagtgt tgtcgggaat ttcgcttgat ggtaaacgct
     3181 acttctatgt caatcctctg gaagacgcag gtaaacgcga gcgagaggaa tggtttgaat
     3241 gcgcgtgctg tcctccaaac atcgctagat tgctgacctc tttcggaggt tatatctatg
     3301 gaacaactct gaacgaaatc agggtcaatt tctacgaaga gagcaaagcc acaattcctt
     3361 ttagagacgg agaagtttcc attattcaga aaacttctta tccacactct gaagaagtgc
     3421 agttaactgt tgctactgat ctggatactg agctctctat tctcctaaga attcctgaat
     3481 ggaccgaagg agaattcgaa gtgcaggtcg atggcataaa acaaaaactt cgtccagaga
     3541 agggatttgt gcggctggaa ggcaactgga agggaaagac tgaagtatat ctggcccttc
     3601 cgatgcgcat tcgattgatg accgccaacc ctcttcttag ggaaaacact gacaaagtct
     3661 cggtccaaag ggggcctctc gtttactgcg cagaaggtgt tgacaacccg gatttcgatg
     3721 taagaacctt gtcagtgcct tcaagaaaga acttcgaagt cacacagagt gatgcactgc
     3781 caggaaaccc agtaattctt agtgggaagg gagtcgcctt tgatctggat ggatgggaaa
     3841 agaagctata caaccccctt ggatcagtga agagcaaggg caacaatgtt agattttctc
     3901 ttgttccata ctacgcctgg aataacaggg gaaaatcgac aatgtgtgta tggttgcatg
     3961 tccactaggg aaagctccat ctattattct ttgggaactc aatgctgtga attcaagcaa
     4021 ccaataagat ccatagacgg acttttcgaa atcgcgagtt cattgaaagg agcgttctta
     4081 catctgtttt gtggcaggag agtgtcttat ttgcgtactt tctttgtgat cataatatta
     4141 gctttttcag cgagctgtct agcttatgaa gggcagattc tatttagcgg aaacgagatc
     4201 cactatgaag tcgaagaagc cgatggagaa gtggttatcc tgattggcga cggtccaggc
     4261 ttcagcagtt cttatatgaa aggaatactc agtggaagga agactttgag atacgatcag
     4321 cttgaaagtg ggagttcaaa gaatcactcg gatgtagcta ttgattttca atactttgtt
     4381 gctgaactcg cttcacttat tttttcgctc gaaataaact cttttcatct tcttggccat
     4441 ggatttggat cagcaatcgc cgttggattt gcccttacaa atcccgaagg actgctcagt
     4501 ctcgtaatga taaatccaag attgaacctc cctgcaatcg attccgcatt atccgatcta
     4561 gccgatggtg aatcgtctca gagtttcgac aagttccatt atttgtttgg ggatggtccc
     4621 acatctgaac tgaagccggt actttccaga gatattataa acgaggaaat ctataacctg
     4681 ttttggggag aaacccctac cttcataagt ggaattctta gaggacatga tttttcctca
     4741 ggacttttag acattaacgt accgattctt atctgctctg gattaaacag ctttcccggc
     4801 gtcaagaatc tcttaacgtt tcaagagggg tctccaaata tagaatttgt aaccttcatg
     4861 aacagcggac atctacctat gcttgaagag aagaaagcct tcaaaaccat tgtggaagat
     4921 ttccttcaaa gagccgaaga aaactttagt catcaaggac aacaggaaat gaatcttgat
     4981 gacatgtcgg aagttccagg agatatggta ttcggatggg aagatcttga gaaagttcac
     5041 gaacttcacg ttggagatga tttttccgtg aagcttcctt caaatcgagg tgcaggctac
     5101 ttctggaaag tcaagagttt agacgaaaac tcgataagac tgatttcgga tccatactac
     5161 gaagagctta aggaatcagg cggtggacgc gacgtcttcg atttccgggt agttggatct
     5221 gggaagtctt ccattgagtt ttcctttggt tcattgtggg aggaaacttc aataaggacg
     5281 tcttcatttt ctattaatgt agaaaaaccc aacatagatc cgataattat tgattcttca
     5341 gatgtgggaa aaactattgt agtcggtctc aatgaaccta tagaggttat tctcgattcc
     5401 cctattgatt ccggattaag atggcgtatc tctgccacca ctcctgaagt actccgacag
     5461 ccaagagaaa atgaagtgaa aattacggag ccatcgccgt attcagccgg gaaagttgca
     5521 caaaccttct actttgaagg catgaattac gggcaggcta ggcttaggtt tgattatggc
     5581 agtctgtggg aagatgtcgc tccagagaaa acctttgagg ctacaatagt cgttgcagaa
     5641 cccgttaggg aatatgtttt tgttcaggat tccgactatg gaagaacgat ttcaataagg
     5701 atcgatcaaa cggtcttggt tagattgaag aaaggcaacg aagaagattg cgaatggcaa
     5761 ctttcttcat attcggcagt agaacctgtt ggtgacatca ctgaagtaga gtatatgggt
     5821 gtaaggtatt cagtgtttcg attaaaaccc acagactcag gagattctat gcttgaattt
     5881 ctctattttg aaggcgaaaa aagaagggaa caggttgatg ccttcaaaat aggcttggta
     5941 ataactgaag aatctggtca agaaatgtcg cccgatcaaa aatagataac ctcacttccg
     6001 gcggagaagg ctaaattacc ggatcagtct cggatctcct tgatatcctt cgagagggct
     6061 atctgcagtt caaggagatt tcatcgaaga ctactctgta gccacttcct cttggactac
     6121 agcaataagc ccccaccata actggaagtt tgcagtcgtc ttctattagt cttgccagcc
     6181 ggagttgaac ccactcatcc tgccaagcaa aaactgagta gtcagatccc tttctgtcga
     6241 ctttaactct taggctcctt gtttcgcaag atacatcttg agtcgaccag tctgaatagc
     6301 cagagttagt aacaacggca cctagtttgg atggggcact tccttcaaac tcaatagagc
     6361 tcttcaacca gcaggaaggt gagaccctca ccatgactcc ggcctgatcg tattgattga
     6421 ccgggaagaa cttcgcgcca aggacaaacg agaaatcttc cattaatggg acaaaaagaa
     6481 aatggccgtt gtcgtttcta aatccgtaat gcgttctttg ccagaaatcg gtagattctt
     6541 ctgattccac tatcaggtta ccatcctgca attcccagcg cttcggttca ttcagccatg
     6601 tgaaatgctt aattgttccc tcgccaaatg aagaaagctg catgacattt cctcccttag
     6661 tctcttgaac tataccgtgc aacacaactg attatttaac agtgagtata taattgttat
     6721 gtaccgtaac ccatcatatc tatttgcatt tgtcggcaga actatttgtt atcattgttt
     6781 atgttgtgtt gtgggtgtga tttacctctg accactgtgt tttcaatcga ttcacaacac
     6841 caatgaactt ttctcatagg cgagatcttt tcggctatga aagctcgtag acttaataag
     6901 gataacatat tctttctact cgtcatttgt ttttgggaaa tacacgtttt tgaatcccaa
     6961 tagagatcga acagaatttt gcttgttgat cgaggtgaat tatgtactgg aagtatgcaa
     7021 ttaagagagt gctctatggg acacttatgt acgcgatctt ggttttcatt ttctctgctc
     7081 tgttcaatac ggtaatggaa cagaccctaa gagcgcaaat tgaagaacag atcagaggag
     7141 agactatgcg gatgaccagt ctgaacgaga gtcagctgga agactacgtt attaatcgaa
     7201 gaaacgatct gtactcactg taccgcctaa caagaccagt agccgaaaga attctctggc
     7261 gaacctggga caccctcact ttgaatcttg gtaattcgac aatcattaga tcctccaatg
     7321 gaagcagaaa cgtatgggat gtcgtttcag aagctatacc gaagaccatt cttctcttct
     7381 ctgttgcaat gctggtggac attgttctcg gccttcttct gggactgaaa aaggcacaaa
     7441 aggccggagg actactggac aaaagcactt cagttggaac catggtggtc ttcggcatgc
     7501 cgtcgtggtg gctgggcatg attatgatca tgttcttcgc ctatggagta aagatctttc
     7561 cctccggtgg actacactcg actccaccgc cagagggaat ttcgtacttc ttcgatcttc
     7621 tgtatcacct cgcgctccca gtacttacgc ttgttgttat cggtttctgg ggaagggcct
     7681 ttcttaccag aaacatcgtt ctcggagtac ttcaggaaga ctacataatg gcggcaagag
     7741 caagaggcat ccctgaaagg aaggttctct acggacacac tatgagaact tctgcaccgc
     7801 cgattgttac aatgtctctg ctggcattgc tggcttctgt tggaggaaac attgtctacg
     7861 agggaatttt ctcctggcca ggaatgggaa acctttactg gatagctttg cagcagaatg
     7921 atgttccagt tcttatggga aacctcgcga taactaccgg cttatatatc tgtggtctag
     7981 tccttctaga tctcatttat ggcctacttg atccaagaat caaggtaggt ggtaaacaat
     8041 gactatgagt gatttaagaa gatcggcaag cgatttctgg aaggaattca gaaaggttag
     8101 gtcgggaatg attggtctca tatttctcgt agtatttatc tgcatcctaa tctttgaacc
     8161 tatcatcgta ccatatcccg aagtcaacga gaagtggaga gatattacct attggcagga
     8221 tcttccttcc agtgcacctc cttcgtggat taatctcttt acctcaaaga agcgcgcggt
     8281 atcggagact atcgagaagt ataccttttc cgaaaccctt gccggtgcaa tgagaataca
     8341 ggagtatgtt tttgaatatg attactccgc gtctgttgct ccctcggatg tcgtgtttca
     8401 tgcttctgga gtcggcaagc caactgtggt tatctctatc gaaagaccgg acggcctttc
     8461 agttgatttg tacagaaaac agcttgaaac ctctccaagt caagatgcca gggtctcaat
     8521 cgatagatcg gcccaaacat cggcgatgaa tttcggcaag aaatactccc cttctgaagg
     8581 agtccctctc caaacaatca agactaccga tgtgttattc tcgaaagccc aggagggaat
     8641 attcagaaat cctgagccgc tcgttggaac atacagactc aaagtaacac ttctactgca
     8701 aaagccagac gagaggatcg acaatgtctt tatcagcatt gcgggcagag tacatgggat
     8761 tctgggaacg gacaactcga agagagatat ctggagtgga gtaatagctg gagtgaaatg
     8821 ggccatgctc atcgggctcc tcacggctct tgtttcggtt tccataggtg tcgtttacgg
     8881 tgtcatcagt gcttatgttg gaggctggaa ggattcttta atgcaacgga tattcgagat
     8941 attcataagc gttcccatgc ttcccgtact cattgtcatg agtgctgtct ttaagcctaa
     9001 tatctgggtg atgattggaa taatgtgcat cttctactgg gtaggaccgg ttaagaccgt
     9061 caggagcatg ggactccaga tcaaggaaga gacatatatt gaggcctcca gggctctcgg
     9121 agcctctaat acgagaataa tcttcaagca tatggttcct ctgctcatcc
//
LOCUS       DAOQYI010000056         9048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_8186_length_9048_cov_74.107528, whole genome shotgun sequence.
ACCESSION   DAOQYI010000056 DAOQYI010000000
VERSION     DAOQYI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8186_length_9048_cov_74.107528"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..739)
                     /locus_tag="PKL93_09675"
     CDS             complement(317..739)
                     /locus_tag="PKL93_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71566.1"
                     /translation="MKRIILITSSMAILLMLLSGCFFIDPMVKVTKLVSDFKKYWEDE
                     NAVGLAGLYTDPALINTAMKSYSQIKDAYSSRFEGRDVIFFNRGDVEVDFNVGATEAT
                     VGFSAVINWTPDPDDHRTYSWTVKKMSGNWFISRSNEY"
     gene            1004..1957
                     /locus_tag="PKL93_09680"
     CDS             1004..1957
                     /locus_tag="PKL93_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNQ71567.1"
                     /translation="MKMLFFMLLSILVIVPTGLAQENVGVLIVGEPDGQYKSIQAAID
                     AANPGDTIRILPGTYKESLSISKEIKLEGSSRDEVTIMPEEGKDLGIFVRGAKNFSIR
                     SVTVIASGAAINVSRSSGQIEDAFIAGGRFGISFSGTGMTLEVLDSHITCYDGMDNEE
                     YIETRLAGIYAYGNATLIAENCVFERNGVGISLTNDLKYKITGCTFKRNTVGVSLGGN
                     ATGSLSENVVTENIENGILINSSSETRLENNIFFGNIWHGLDLYLNRCTECECGGDEF
                     NGTVVGSGNRFESEDEICPIDFWDESFYSFDENLGKLADKE"
     gene            2225..2404
                     /locus_tag="PKL93_09685"
     CDS             2225..2404
                     /locus_tag="PKL93_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71568.1"
                     /translation="MRKMRRDRFYKTVLIVFTIGLAIAVMTMVIALINLTDANTSSKA
                     FREYLDESNVIESNP"
     gene            complement(2870..3013)
                     /locus_tag="PKL93_09690"
     CDS             complement(2870..3013)
                     /locus_tag="PKL93_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71569.1"
                     /translation="MLSLHGTLQMQGKYGEMAEWIGTNGFAPTGSVYENYYNGPEFPE
                     SKY"
     gene            complement(3724..5340)
                     /locus_tag="PKL93_09695"
     CDS             complement(3724..5340)
                     /locus_tag="PKL93_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71570.1"
                     /translation="MFRKALIAIVLVASVFAFSTQFKRTIEDKIVVQHDGSAIITRTE
                     YVPASDLSKVYIQHRDALRERETADEAFANFYDEISKGYFFLYRSAPGFGDNDVKFKI
                     EGNGNVTSTISMKVTGLISESKEHPGSYQFSSKNFSEEKIMLKYLEDLIDGKAFETAF
                     LGSSKNSLLTTKTTTIVLPEGSEIIDLLSPHGEKPSKDWSIDLGGGTKFKASLDLEKN
                     TIILKEEITTGGGAPKNLMNEDNEELMAKLRDYAAYIAVFNNEKIGGKLSQPEPYFFK
                     EDYSGSWNFGISHNFSTQFAYQTLSVEPGLNVSFTFGTSLLWEHQWKKVSWWKYKYVL
                     KKFQTTVSLSPSLTPYIEVSSGAALSKTWEKNITTKTKWITFWVSCVPVTLVMEVKFD
                     AKAEAGISGVIGFTASTTLSANTTLTVKYENGWSKNVNYSANYSGLQFDADAKVNAWA
                     KGSLPLTLSAYVYYVAGPFVQFVPWLKGETNASAGSSTQVGYKVTAGFDVNGGVHMAG
                     WLKDLCDGVPSVSYTFWNKSWTIANSTLTF"
     gene            complement(6037..6456)
                     /locus_tag="PKL93_09700"
     CDS             complement(6037..6456)
                     /locus_tag="PKL93_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71571.1"
                     /translation="MILGIFLKDREVSFFDGCLEVKIDRMEAFYAKVVLGGETPGVSF
                     EDYMTTASVIVKRDENNMVILDFEDFAPPGEIRELRFTADDITLSVTLEDKETEEISQ
                     GFRDYRPPEDFLSYLKRNMKKSDNPLVTGDVEQVASC"
     gene            7138..8346
                     /locus_tag="PKL93_09705"
     CDS             7138..8346
                     /locus_tag="PKL93_09705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71572.1"
                     /translation="MEIRRIDSSYKDKIVEIFLMSFEYFEPEESEFFFSDPSLWKYVW
                     GAFDDEKLVAAYISYKYLAKIRTKPFECRYVEAVATLPEYRNKGITKAILLKDIQEAL
                     QSGVNFLMLDPFKHDFYVRFGFGLGFESLDFKFDFSLLSDEIEEDNLNIVSGKLLNSA
                     EMQELIEKARKVLWETSRYTEAIEINACSQEIFHMKDLFGAVAVDKAGMPHAMMVYRK
                     KERKMKVSNFSFIDLAGLFALKRFMLNHRDQIATFEMNRMPPDFPVELFFHSRWQAGK
                     DLKIEDASSRMVRILDPLPVIKELMNRSISETVVIKIKDKLLPQNNLTIEIGKGTARL
                     SEAPNDLEITISDLTPLLTGRLSPTRLWRHGRLRTDSWKNVPWGISEVPEKVKVLESI
                     FPPFITHNAN"
     gene            8380..8898
                     /locus_tag="PKL93_09710"
     CDS             8380..8898
                     /locus_tag="PKL93_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HNQ71573.1"
                     /translation="MRGKPVIVAEYDSSWPELFRREAELIYAALEGVEMSIEHIGSTA
                     VPGLQAKPVIDIMIGVSSLEQADSCVPFIERTGYLYRPEHEDSMPERRYFERSGSEID
                     CHVHLVVFGSKFWRGHIFFRNCLRENPEIVQQYAALKKELAEKYRDNREAYTNGKAEF
                     IQGILRRQKDPL"
BASE COUNT         2521 a         2107 c         1970 g         2450 t
ORIGIN      
        1 aacgaatcga attctgggca gaatcatgtc tgaataacgc aaagaatgtc atcccaaact
       61 tgtttcggga tctggtttcg atcctctcgg cgggggacgg aggacctttg acggacaacg
      121 ttgtcttcac agcgatcagc gggttctttc tcttaagctt acagcggttc ttatccttgc
      181 aactgctctt tcgaaggacg ggtccatgat cttggacgaa agaccaagga cggctcttca
      241 cagcgtaaaa aatcaattag gctcaatgca gagtcagtat tctttctatt ctgctaactc
      301 ctgcagaaaa aatcgactag tattcattgg atctcgagat gaaccagttg ccggacatct
      361 ttttcactgt ccaggaataa gtgcgatgat catcgggatc aggtgtccag tttattacag
      421 cgctgaatcc tactgtcgct tctgttgctc caacgttgaa gtccacttcg acgtctcccc
      481 tgttgaagaa gataacatct cttccttcga atctgcttga ataagcgtct ttgatctgtg
      541 aataagattt cattgccgtg tttattagcg caggatctgt atataatcct gcaagaccga
      601 ctgcattttc atcttcccaa tacttcttga aatcgctaac aagctttgta acttttacca
      661 taggatcaat gaagaaacat cccgaaagaa gcatcagtag aatcgccata gaagacgtaa
      721 tcagtatgat ccgcttcatt ttctcgcctc cagctttcac ttggcctctg agaactcatg
      781 aaaaaccaca acggaccacg aattttcctt acaacaagtg attttagcac cattccaaat
      841 agaacagtca cgagatgtaa actagacaat ttaagaaagc gagtcaaatc attggcaaaa
      901 cctcggctat atggttatct gtaatgaccc gatagtgaaa accatcgaat acttgaacca
      961 aattaagata aactaatggt aggaaaggaa tgggggtgcg tttatgaaaa tgcttttctt
     1021 tatgctttta tctatacttg taattgttcc gacaggcctg gcacaagaga atgtcggagt
     1081 tctaattgtc ggggaacctg acggccagta taagagcatt caggcagcta tcgatgctgc
     1141 aaatcccggc gatacaataa gaatacttcc gggaacatac aaggagagcc tttcaatcag
     1201 caaagagata aagctagagg gctcctcaag agacgaagta acaataatgc ccgaagaagg
     1261 aaaggatctc gggatcttcg taagaggtgc caagaacttc tcgataagaa gcgtgactgt
     1321 catcgccagc ggcgcagcca taaacgtctc caggtcctcc ggccagatag aagacgcctt
     1381 catagcgggc ggccgctttg gaatctcttt cagcggcaca gggatgactc tagaggttct
     1441 cgattcacac atcacctgct atgacggcat ggataacgag gagtatattg aaaccagact
     1501 ggccggaatc tacgcgtacg gtaacgccac cctgattgcc gaaaactgcg tcttcgaaag
     1561 gaacggagtt ggaataagcc tgacaaacga cctgaagtac aagatcacag gatgcacttt
     1621 caagagaaat acagtagggg tttcacttgg tggtaatgct acgggatctc tatctgaaaa
     1681 cgtagtcacg gagaacatcg aaaacggtat cttgatcaac tcatcttccg aaaccaggtt
     1741 ggagaacaac atcttcttcg ggaatatttg gcatggtctc gacctctatt tgaatcgatg
     1801 cacggagtgt gaatgcggcg gcgatgaatt caacgggacc gttgtcggtt caggaaacag
     1861 atttgagtcc gaagatgaga tctgcccgat agatttctgg gatgaaagct tctacagttt
     1921 cgatgagaat ctgggcaagt tggctgacaa agaataatca agagtatcac ctttcgattt
     1981 gctccaatgt accggcaact atgttctcca tatcactgcg aggagcattc gcaacattct
     2041 tcttcccaag actttccaaa aaacggttcg atttcatata attcctttgt atatctatta
     2101 tttaagttca catagtaatt caacactaaa cttcctttca ggaatagatt tacacactaa
     2161 ctgatagtta attacagatg catctctcaa ttaagcatgg tatactaatg ttcgaaggag
     2221 cgagatgcgt aagatgagac gggacaggtt ttataaaaca gtcttgatag tctttacgat
     2281 tggtttggcg atagcggtga tgacaatggt aatcgccctg atcaacctta ctgacgcaaa
     2341 cacctcttca aaagctttca gagaatatct cgacgagtca aacgttatcg agtcgaaccc
     2401 ctaagaatct ctacgattct gctatttcta ggcctacgga ttggcacgta tctagctaat
     2461 tcatcccata ctgtatatct caaaccaaag aataatctca tgcaaagtca ttagccagtt
     2521 tctcttgctc tcttgcgtaa gaattctgca agtagaaaga ttcagagaac attagctttc
     2581 tatgatcctc ctcatagatc ttcaagccga aatttgtgta accaattctc gaaaaaggca
     2641 atccttaact agcaatgggt tgagctccta ctggagcaaa atcagttgac aagaacaaga
     2701 acagaggccc tgaaagaggc ctctgttaac taactatccg attctcattt aagtatttcc
     2761 ataggcaatc tgcagaacta tactgaatct aggtctgctt cattctcatc caggaagtct
     2821 tctaagaccg atgctcactc aggtcttctt acatgcatcg aaatcttcgt caatacttac
     2881 tctccgggaa ctcgggcccg ttgtaataat tctcatagac actacctgtt ggtgcaaatc
     2941 cgttcgtgcc tatccactcg gccatttcac cgtatttacc ctgcatttgc aaggtcccgt
     3001 gtaagctgag caaacgtata gacctcccgg aatcgatccc ctaatggtat ctcctcttcc
     3061 cggaagatca gcggagatgg gaaatccaat ctccacttca aggttctcca tatccatgtt
     3121 gtgataagct acaggaggaa tccctgccgg aaatcccttc gttttaccca gaataggccg
     3181 ctatctctcc gtagtacctt cctattatag tgggaagatc ctgaacattc gtccaggctc
     3241 gtataaacag cgctggttgc tcttcctgct tcaatactgc aatcgacgat gatctggtat
     3301 tccttcctcc ttgcatccct tagtaattgt cttctcagaa cggaccctct cgataactgc
     3361 ctaacaaaaa attagatact gattacatca gctagaatac tctttttcaa cgaattccct
     3421 atgacttcga cagcatcgca cgttgccatc catccacgga caagtcacct taagcaagac
     3481 tagatctgct cctgtgcaac acggatatct ttcgccgaaa agtcaaaatc atctactttg
     3541 cgaattctgc atccagcaaa tagcattgca atttgtgctt cttggatcta aggagcttga
     3601 agcgcaacgt taaacaatat ctctgggaat cggtcaattc ttacagtatc tctacagcag
     3661 gcgattcata agaaatccca atcttgacaa agagccctgt taatatttga cggtcatatt
     3721 gctctagaaa gtgagtgtcg agttagcgat tgtccagctc ttgttccaga aagtatagct
     3781 tacactcggg acaccgtcac aaaggtcctt gagccagccc gccatgtgta ctccgccatt
     3841 gacgtcgaag ccggccgtta ctttgtatcc aacctgggta gagctaccgg cagaggcgtt
     3901 agtctcgccc ttcagccatg ggacgaactg cacgaatggg cccgcaacat agtaaacgta
     3961 tgccgaaagg gtaagaggaa gagatccctt tgcccaggca ttgacctttg catctgcatc
     4021 aaattgcaga ccggagtaat ttgccgagta gtttacattt ttactccatc cattttcata
     4081 tttgacagtt agcgtcgtgt ttgcagaaag cgtcgtagaa gccgtgaaac ctataacacc
     4141 tgaaattcct gcctcggctt tcgcatcgaa tttgacctcc ataacgaggg taaccggcac
     4201 gcatgaaacc cagaacgtaa tccatttcgt cttcgttgtg atgttctttt cccatgtctt
     4261 gctcagcgct gcgccggaac tcacttcaat ataaggcgtc agagaagggc tgaggctcac
     4321 ggtcgtctgg aatttcttca gaacgtattt gtatttccac cagcttacct tcttccactg
     4381 gtgctcccag agcagagaag tgccaaatgt gaagctcacg ttaagccctg gttcaacgct
     4441 caaagtctga tacgcgaact gtgtcgagaa gttatgtgaa ataccgaagt tccaagaacc
     4501 tgaatagtct tccttgaaga aatacggctc cggctgcgac agtttaccgc cgatcttttc
     4561 gttattgaag accgctatat atgcggcata gtctctgagc ttcgccatga gttcctcatt
     4621 gtcttcattc atgaggtttt tcggagcgcc tcccccggta gttatttctt ccttcagaat
     4681 gatagtattc ttttcgaggt ctaagctcgc tttgaactta gtccctccgc caagatcgat
     4741 cgaccaatct ttggaaggct tttcaccatg cggagagaga agatcgatga tctcggaccc
     4801 ttcgggtaag acgatagtcg ttgtctttgt cgtaagaagc gagtttttcg acgatcccag
     4861 aaaggccgtc tcgaaggctt tgccatcgat gagatcctcc aaatatttga gcatgatctt
     4921 ctcttctgag aaattcttgc tagagaactg gtagcttccg ggatgctcct tcgattccga
     4981 aattaggccc gtgaccttca tcgaaatcgt actcgtgacg ttcccgtttc cttcaatctt
     5041 gaacttaaca tcattatctc cgaagccggg tgccgatctg tagagaaaga aatatccctt
     5101 gctaatctcg tcatagaagt tagcgaaggc ttcatcggcc gtctctcttt ctctgagggc
     5161 atccctgtgt tggatgtata ccttagaaag atcggacgct ggaacatatt cagtacgggt
     5221 gatgatcgcc gatccgtcgt gctgaacaac gattttgtct tcgattgttc gcttgaactg
     5281 tgttgaaaag gcaaatactg aggccacgag aacaatcgct attagcgcct tcctgaacat
     5341 actttcaccc cctttagaag tcgttcgaac gagtatagca ccggctactt tttgaaatat
     5401 acaaatagaa agacagtcga ctatttggat tgtcttattt agattttgac ggtgttctaa
     5461 aatcaatgct tctcacaaac gcttcttaag ttctagtaga acccgctctc actggaaaat
     5521 caacgtcctc tatcgtttat ccaaaaattc cctttccggg ttcccgtgaa ttgtttagaa
     5581 agtattgact gcaaaaaagc aggctttagt tgaactaatt ccgacagaat gaactgtaga
     5641 attatctctc aagaaatagc atatgaccga gcaattcttc gtgaaatcaa atggattttg
     5701 agagatggat cgttggatcg tttttcacct ttgctgatct tcatccatct tgattcgatt
     5761 catgaagatt ctctggctga ttcccggtta atataattca aaagcgcaaa ggaaggccct
     5821 gctgaccaaa tgcagtggcc ttctcacgag acttgcaatc cgtaaagcaa attgggaaat
     5881 tgaagtcgct ttctgacatg gtagggagat aatgaagaag tcgatcggag acacaaacaa
     5941 gataggtatg ccgcaaatat agctttgctt agtatcactg agtttctcct tgttgttctt
     6001 tcgttcagat ggatcaacag accgttgata tttcaatcag cagcttgcta cctgttcgac
     6061 atctcccgtc acgagggggt tatcgctctt tttcatattc ctcttgagat aagataggaa
     6121 atcctccggt ggacgataat ccctaaaacc ctgcgagatt tcctccgtct ctttgtcttc
     6181 aagggtaact gacaatgtaa tatcatccgc cgtgaaacga agctctctaa tctcgcccgg
     6241 aggggcgaag tcttcaaaat cgagaatcac catattgttt tcatctctct tcacaattac
     6301 tgacgcagta gtcatgtaat cctcaaaaga aactccaggc gtttcacctc ctagtacaac
     6361 ttttgcgtaa aaagcttcca ttctgtcgat cttcacctct aaacaaccat cgaagaaact
     6421 aacctctctg tccttcagaa agatccccag aatcactttc aacctccttc agttaaccgg
     6481 tcagcagata gagacgaccc tctttttttg ccgcaaccgc catcggtttt catttcattg
     6541 gatagaggac tattcaatct cttctgtttc agctttcgat agcttttctg acctttctcc
     6601 ttcctgcctc cttggtcatc ttcaatcaag acttgattct gaatccccat tgttgactca
     6661 acatcgaacg ccagttctgc aaacttcgga attacggcag caagaaaggc cggaaacggt
     6721 cttaccaaat ccgagtaatc tggcggaata ctactggtcg tttgcagaac taatcgggga
     6781 caatacacaa ctacgatata tatgctacga ttcgctttct caaaatttac cgttatgttt
     6841 actaagattt attctggcga aaatactgca actgtcaaaa acaaaatgct tatcgctaag
     6901 agttttgttg cattgcgctt tttaagctca gaaaacgcag ctttcggacg aatgtacctt
     6961 tctttatctg ctcctgaatt gcgagcttcg taatcggggt tgttgtttgc ccatattgaa
     7021 agatcgacag aaacatatag aaaactcgag aattgagaag tctactaatc aagtctataa
     7081 agagcctgag agagccgatc tggtatcatt ggtagagatg attgtttgag gtgcactatg
     7141 gagataagaa gaatcgacag ttcgtacaaa gacaagattg ttgagatctt cctgatgtca
     7201 tttgaatact tcgaaccgga agagagcgag ttcttctttt ccgatccgtc tctctggaaa
     7261 tacgtctggg gagcattcga tgatgagaaa ctggttgcag catacataag ctacaagtat
     7321 ctcgcgaaga tccgcaccaa gccttttgaa tgcaggtacg tcgaggctgt ggcgacgctg
     7381 cccgagtatc gcaacaaagg aataactaag gcaattctcc ttaaagacat acaggaggcg
     7441 ctgcagagcg gcgtcaattt cttgatgctc gaccccttca agcatgattt ctatgtgagg
     7501 tttggattcg gccttgggtt tgaaagcctg gacttcaagt tcgacttttc tctcctttcg
     7561 gatgaaattg aagaggacaa tctaaatatc gtttccggca agctcctgaa tagcgccgag
     7621 atgcaggagc tcatcgaaaa ggctagaaag gtcctctggg aaacctctcg ctacaccgaa
     7681 gccattgaaa tcaacgcctg cagtcaggag atcttccaca tgaaagacct cttcggggcc
     7741 gtggccgtag acaaggcagg tatgcctcat gcgatgatgg tttacagaaa gaaagagaga
     7801 aagatgaaag tcagtaactt ctctttcata gaccttgcgg gtctcttcgc actgaagaga
     7861 ttcatgctca atcacagaga ccagatcgcg accttcgaaa tgaacaggat gcctcccgat
     7921 ttccccgtgg aactcttctt ccattccaga tggcaggccg gaaaggacct gaaaatcgag
     7981 gacgcctctt cgcgaatggt gagaatcctt gatccattgc ccgtgatcaa ggaacttatg
     8041 aacaggtcaa tttccgaaac agttgtcatc aagataaaag acaagctgct gccccagaac
     8101 aatctcacta tcgaaatcgg aaagggtact gcaaggttga gtgaagctcc taatgatctg
     8161 gagattacga tttccgacct tacacccctc ctgacaggaa ggctttcacc cacaagactc
     8221 tggagacatg gaaggcttcg tacggactca tggaagaacg ttccttgggg gatctccgaa
     8281 gttcctgaaa aggtgaaggt cttggaatcg atctttcccc cgttcattac ccacaacgcc
     8341 aactaacgtt caattgaagg acagttgagg agggatccct tgagaggaaa gccagtgata
     8401 gtcgctgagt atgattcatc gtggccagag cttttcagaa gggaagcgga actgatctat
     8461 gccgcgctcg aaggtgttga gatgagtatc gagcacatag gaagcacagc agttcctgga
     8521 ctgcaggcca aacccgtgat cgacataatg attggcgtat catcacttga acaagccgat
     8581 tcctgcgtgc cattcattga aagaacgggc tatctttaca gacccgagca cgaggattca
     8641 atgccagaaa gaaggtactt tgaaagatcc ggcagtgaga ttgactgcca cgtacacttg
     8701 gtcgtttttg gaagcaagtt ctggagaggc cacatctttt tcaggaactg tttgcgtgaa
     8761 aacccagaga tagtacaaca atacgctgcg ctcaagaaag agctcgccga aaaatacaga
     8821 gataacagag aagcttatac aaatggcaag gctgagttca tccaggggat actgagacgg
     8881 cagaaggatc cactttagga aactaacgtt ttgaccacag cgcccgtagt ggaaatggaa
     8941 acgcgcgtag aggttagagg tcagtggtca gaggttggaa gagcaagaga gtggtcaccg
     9001 ttcgtcgagg agaagttgtt cgttccagag agaggatctg ttcttcat
//
LOCUS       DAOQYI010000057         8897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_8332_length_8897_cov_82.885207, whole genome shotgun sequence.
ACCESSION   DAOQYI010000057 DAOQYI010000000
VERSION     DAOQYI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8332_length_8897_cov_82.885207"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..1530
                     /locus_tag="PKL93_09715"
     CDS             289..1530
                     /locus_tag="PKL93_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HNQ71574.1"
                     /translation="MRLKRVSIVIVAALVAMLMISSCIPKDPVVAATRKFIQFIEEEG
                     EPEGPFTGLYLGEVVKGDVIGSESAGGQQLQYQLQGINEAGYFFYLDEAPGAFYDHPG
                     KLVVISKGGQAIFEEDTEGWPTINGNIVSPLSDRNLYAKAVIWDKWKYVREIITGIVV
                     DWTQVFFRIKGAVITSGLTPTQNLYAEARDVRDLMSTAFKTIMGTDKVKDVKYLSGAA
                     LPNWTAVRAAMDDLFTTKKVTDMTLYFIAHGNTNLMNLGGTTFYANQLRSYILEHSSK
                     RFLIIIESCHSGSWLEGLKSDGVIPSNVEIIITTTTATKGAYPDWDSAGGAVDYNPDD
                     MYVEWSGDFLQKLSSFTGTGWPEVTSYASSKSIDTLPALLYKCYTAIKGGSPSTTSWT
                     LTERSVAGPVQQPMIYTKWTP"
     gene            1636..2106
                     /locus_tag="PKL93_09720"
     CDS             1636..2106
                     /locus_tag="PKL93_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71575.1"
                     /translation="MVKPEKQKGYLVRLKVLKDETDLLRVEIELYKTSTHPVIMDSLF
                     DTSIIRASKLVRNSGFTMKSFREYIRQGCPKHFRRELYRIMDDFDREEALLAERIKKL
                     KNRRDRVIVHMDPRFAFHPEREDENRVDLEDIEAICLHLERQVAFFSGKTLDEG"
     gene            complement(2530..3219)
                     /locus_tag="PKL93_09725"
     CDS             complement(2530..3219)
                     /locus_tag="PKL93_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ71576.1"
                     /translation="MKAIAFNGSPRMERGYTSRLLSSLLKGIQSAGCDTELFYTNTFS
                     PLLCTCGSMHCWDIEPGICCMKDSMQPIYEKIKESEILVLAAPVYIPLSGAMQNLINR
                     LCPMIDPVLSIVDGRTRAKLRAGWKTKKIMLVSTGGWWEKENFDTLIRIVRELAENAS
                     VVFSGALIRPHVHLMFDRGDPTDEGKEILLKVEEAGYELVRKETIDDDLLKFISRPLI
                     SHETFVEMWNR"
     gene            3494..3697
                     /locus_tag="PKL93_09730"
     CDS             3494..3697
                     /locus_tag="PKL93_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ71577.1"
                     /translation="MKNRLKEFRFKNGEMTQDNMARIAGVSRQTIIAVEKGKFNPSAK
                     LALKLANILHCTVEELFELEEGD"
     gene            3698..4342
                     /locus_tag="PKL93_09735"
     CDS             3698..4342
                     /locus_tag="PKL93_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71578.1"
                     /translation="MLLKTGILIFDVFIIILSVSLIRTAGGWYSLLLLGLYLFPSLRM
                     LRLIPGFDEREKAIDSFSSNMALGISFVLAIFYIGMSYTLTIDAAISFILIPFVAKAV
                     FFSGFTLERKQLVSIIGRSVGLIFVGFALFSHGFSTVGLIEMLPGIVIIICTELSRRY
                     RWFGLSYLFLIGIATWVYLPNFGRITSVLVYSLFVIPFSFLLVRSLMNTLNYEL"
     gene            complement(4339..5850)
                     /locus_tag="PKL93_09740"
     CDS             complement(4339..5850)
                     /locus_tag="PKL93_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNQ71579.1"
                     /translation="MRYSRVRTVTTVGLKVLDVLVELDIDSRAVIQEMDIVGLGDTVI
                     KESKKRVKSAVKNSGIELPNGRITVNLAPSDVKKEGSYLDLPIAVGLLKATSKINADL
                     SDFVFFGELGLDGSLRRVRGVLPILLSLSRRAKGMKIVLPAPNRQEASIVDNLTIFTA
                     DNLNDLVGFLNGYIEKSPIAYSQPELGNVSGPDMSEIKGQEFAKRASEVAAAGGHNIS
                     LRGSPGCGKTMLARRIPSILPPLTMEEAIETTMIYSVAGLLGEKGLIKERPFRSPHHT
                     ASTTSIVGGGNDAKPGEISLAHNGVLFMDEFPEFRRDVIEALRQPLEDGVITVARAKL
                     TATYPARFMMVAAQNPCPCGWYGDKTRECTCSWYDIQRYNRKISGPMEDRIDIFVDMP
                     RLDFDKYMENSTTESSDEIRKRVIAARKRQSERYEGMSIFSNSQLGHALLKEYVPLDT
                     ASRSLLSAAMDKMKLTARAIDRVLKVALTIADLEESKVEARHIAEALQYRRKT"
     gene            5939..6400
                     /locus_tag="PKL93_09745"
     CDS             5939..6400
                     /locus_tag="PKL93_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ71580.1"
                     /translation="MKNTHRIKVRALIIKNESVLLVRHEHHDRPPFWCFPGGFVESDE
                     DLFSAIKREIREETQVVVSPRSVIALQEFKRESLLEVIFSCDYVSGKLKLGSDPDNPG
                     IPTLVDAKWVRIDELDRFKILPVQLASLLKNSWGNLCSVELFQIETMREVP"
     gene            6496..7029
                     /gene="raiA"
                     /locus_tag="PKL93_09750"
     CDS             6496..7029
                     /gene="raiA"
                     /locus_tag="PKL93_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNQ71581.1"
                     /translation="MDYNIYSKDVTVTDSMKDYIDKRFSKVSKVVNEEKLISMDLRLS
                     KERAFYKIEATAHLPGVMVRVEEKGSDFYTVVDSSSDAFERRIKRFKERTRYKHKNGL
                     KENLDSIPSKFEEDHSTMITRRKKFTIKPMTEEEAVLQMEMLGHTFFVYRNIDTDEVN
                     VLYTRKNGSIGLIEMTE"
     gene            7131..8603
                     /locus_tag="PKL93_09755"
     CDS             7131..8603
                     /locus_tag="PKL93_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNQ71582.1"
                     /translation="MARIQNNRTQTIVFSRIEDEVRIASLDGDQLEEIFFEDMDSESV
                     TGNIYLGKVENVVPSLEAAFVNIGIGRNAFLRFKDASGQPKLEKNNKVLVQVSKDPIG
                     SKGPQITLKVSIPGRSLVYTPFSKHIGISKKITDQQERQRLTAVAKSTLENKEGVIFR
                     TAAFGVGDETIKEELESLRTNWNQICETFRRSRKPKILYEEPSFVEYILRERLTENTK
                     EIVVDSEELWHDVVAGISKFKSGFKPVVRYVEGDAFASEEVYEKMKILYTRMVSLPGG
                     GNIYIDRTEAMTVIDVNSASNVSGNDVSETSLNTNLEAASEIARQLKLRNIGGIVVID
                     FIDMKSDEDRQKIISSLTGEFKKDKARTMIMGFTRLGLLEMTRKRSSAAIGSKLYSSC
                     PICKGTGRIESPRAVYQRLLKDLARGFEDETISSATVQVYQNMSGILTPENQKNLAKS
                     LKREISVGFTWPIPTSYEIKFAKKQEKPKRTRRKTKKESN"
     gene            complement(8619..8693)
                     /locus_tag="PKL93_09760"
     tRNA            complement(8619..8693)
                     /locus_tag="PKL93_09760"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8659..8661),aa:Val,seq:cac)
BASE COUNT         2585 a         1953 c         1938 g         2421 t
ORIGIN      
        1 aagagggtgg aaacaccctc tttttcattt tgaaatatct gctttctctt aaaagtaccg
       61 ctgcgaattt acttccctct ttttgttttg ttattactac gatactcacg ttaaacacag
      121 aatcttacaa gaaaaactct aacaatcgaa tttataacgt aatcgatcgt tgagtcaata
      181 taaacgtaaa tatcgttctt tcaatactat aatctgcctg cacaatgact tattgacata
      241 cagcttgaat atgaacagga aagctccgca tgacgggaag gtgatattat gcggctgaaa
      301 agagtatcga tagttattgt tgcggctctc gttgcaatgc ttatgatatc ttcctgtatt
      361 ccaaaagatc ctgttgtagc tgccacaaga aagttcattc agttcataga ggaggaagga
      421 gaaccggaag ggccatttac cggactgtat cttggagaag ttgttaaggg cgatgtcatt
      481 ggatctgagt cggctggagg ccaacaactc cagtatcagt tgcaagggat aaatgaagca
      541 ggttatttct tttatctgga tgaagctccc ggtgcattct acgatcaccc tggaaaactc
      601 gtcgtcattt ctaaaggcgg ccaggctatt tttgaagaag acaccgaagg ctggccaacg
      661 attaacggaa acattgtttc acctttgagc gatcggaatc tctatgctaa agcagttatt
      721 tgggacaagt ggaagtatgt cagggaaata atcactggaa ttgttgttga ctggacacaa
      781 gtgtttttca gaataaaggg ggccgtaatt accagtggac tcactcccac acagaatctt
      841 tacgcggagg cccgagatgt aagggatctc atgtccaccg ctttcaagac aataatggga
      901 actgacaaag taaaggatgt gaaatattta tctggcgcag ctttaccaaa ctggactgct
      961 gtgcgagctg caatggacga ccttttcacc acaaaaaagg ttaccgacat gaccctatat
     1021 tttattgcac atggaaacac gaacctcatg aatctcgggg gaacgacttt ctacgcaaat
     1081 caacttagaa gctatattct cgagcactct agcaagaggt tcctaataat aatcgagtct
     1141 tgtcattctg gtagctggct tgagggtcta aaatccgacg gagtaatccc ttcaaatgtc
     1201 gaaataataa taactactac taccgctacc aagggtgcat atccagactg ggacagcgct
     1261 ggtggtgcgg tggattacaa tcctgacgac atgtatgttg agtggtcggg tgacttcctg
     1321 caaaaactct cttcctttac cggcacaggc tggccagaag tcacatcata cgcctctagc
     1381 aaatccatag acacactgcc cgcattgttg tacaagtgtt atacggcaat aaagggtgga
     1441 agtccttcga ctacgtcatg gactttgaca gagcgttctg tggcaggccc tgttcagcaa
     1501 cctatgatat acacgaagtg gacaccttaa ggttattatt gaaaagtaag tgaagagcgg
     1561 gtgattcccg ctctttattt ttctcctagc tttgttcttc gcaaatagca taattgaaat
     1621 atggatgttc tttctatggt caaaccggaa aagcaaaagg gttaccttgt aagactgaag
     1681 gttcttaaag acgagactga tcttctcagg gttgaaatcg agctctacaa aacttcaacc
     1741 caccccgtga taatggattc acttttcgat acctctataa taagggcgtc caaactggta
     1801 cgcaacagtg gcttcaccat gaagagtttt cgagagtata tccgacaagg ctgcccaaaa
     1861 cactttcggc gagaattata tcgaataatg gacgattttg atagagagga agccctcctt
     1921 gcagaacgaa ttaagaaact aaagaatcgt cgtgatcgag tgattgttca catggatccg
     1981 cgttttgcct ttcatcccga gcgagaagat gaaaacagag tagatctaga agacatagaa
     2041 gcgatatgct tacatcttga aagacaggtt gctttcttca gtggaaagac tctagacgaa
     2101 ggatgaagtt agcgttagag cacgtatgga gcatccgcag aacttcagtt gaatcctatc
     2161 tccgatgaac gaacccagta aaccgaaacg gccagcaaga gttatcagca gtgaaacact
     2221 tcggatgaca cacacctaat gaaagaataa ccgttaaaca actattcgaa gaattcccct
     2281 ttgacaaatg cagatcagaa ttcacgatcg atttgttctt tttctatgta taagaatctg
     2341 caagaatatg caaccaagag taatatctac aagctatgtg cccatgagag atctcataac
     2401 gtatgataca agaattacca gaaaactatc acttttcgaa catgaagcgc tcacttctat
     2461 caacacttac tgagttcccc aaagaaaatg tctcctatgt agattcatga atgttttccc
     2521 ggagaatcgt cacctgttcc acatttcgac gaatgtctca tgagagatca gcggtcggct
     2581 aatgaatttc agaagatcat cgtctattgt ctcttttctt acgagttcgt agcccgcctc
     2641 ctcaactttg agaagtatct cttttccttc atctgtcgga tctcccctgt caaacattag
     2701 gtgaacatga ggcctgatca aagcaccgga aaacactacg ctagcattct ccgccaattc
     2761 acgtacgatt cttataagag tgtcgaagtt ttccttttcc caccagccgc ctgtagatac
     2821 cagcataatc ttcttcgttt tccagccggc tctcagcttg gcccgagtcc tgccatcaac
     2881 aatacttagt actggatcta tcataggaca tagcctgttt atgagattct gcatcgctcc
     2941 cgacaacgga atgtaaactg gagctgccag tacgagaatc tcagattctt tgattttctc
     3001 ataaatcggt tgcatactat ccttcatgca gcagattcca ggctctatat cccagcagtg
     3061 catacttccg caagtacaaa gtagtggtga aaaagtgttt gtgtagaaga gctcagtatc
     3121 gcatcccgcg ctttggatgc cctttaagag cgaagataac agtcttgaag tgtagcctct
     3181 ctccatcctt ggacttccat tgaaggcaat tgctttcact atttcacctc ctcaaacgag
     3241 gattcacaga cttgaaagca agatcaatct tttctaagat tccagatttc atgtatccac
     3301 cccccggaac atctcaaaaa caatctagtc ttttagtctt atagtgtgac ttatgcgtta
     3361 tgacaaaaga aactctttac tcctttcttc aaacgttata tctttacctg agtaggctga
     3421 tctacaaaga tgaaatgttt ataatacttg acattatgta aagtgttatg tacacttgac
     3481 ccaggtgatg gctatgaaga acagattgaa ggaatttaga ttcaagaatg gagaaatgac
     3541 acaagacaat atggcaagaa tagctggagt aagccggcag acaataattg cagtcgaaaa
     3601 aggaaaattc aatccttcag caaaacttgc tcttaagctc gcaaatatcc ttcactgtac
     3661 tgtcgaagaa ctctttgaac tggaagaggg tgattaggtg ttgcttaaaa cgggtatctt
     3721 gatatttgat gtcttcataa ttatcctatc agtttctctc attagaacgg cgggaggctg
     3781 gtattctcta ctcttactgg gcctctatct ctttccttca ctgaggatgc tccgattgat
     3841 ccccggattt gacgaaaggg agaaggcaat cgacagtttt tcatctaata tggctttagg
     3901 aataagcttc gttttagcca tcttttatat tggtatgagt tacaccctta cgatcgacgc
     3961 tgcgatctct ttcatcctta ttccttttgt tgcaaaggcc gtctttttct ccggattcac
     4021 ccttgagaga aagcagttgg tttcaatcat tggaagatcg gtgggcttga tatttgtagg
     4081 attcgctctt ttttcccacg gtttttctac ggttggtttg atagaaatgc tgcccggtat
     4141 cgttattatt atttgcactg agctttctcg cagatacaga tggttcggtc tcagttatct
     4201 gttcttaata ggtattgcaa catgggtcta tttaccaaac tttggaagaa tcacgtcggt
     4261 gcttgtatac tcattgttcg tcattccgtt tagttttctt ctggtaagat ctcttatgaa
     4321 taccttgaac tatgaactct aggtctttct tcgatactgc aaagcctctg caatgtgcct
     4381 ggcctcgact ttgctttctt cgagatcggc aattgtgagt gctactttta gaactctatc
     4441 aatggccctt gcggttaatt tcatcttatc catggccgcc gaaagaagtg atctcgatgc
     4501 agtatcgagt gggacgtatt ctttcagcaa ggcatgacca agctgactat tcgaaaatat
     4561 gctcattccc tcatatctct ccgactgtct ctttcttgcc gcaatgaccc tcttccgaat
     4621 ttcgtccgag ctttctgtcg tcgagttttc catatacttg tcgaaatcta gtcttggcat
     4681 atccacaaag atgtctattc tatcttccat tggacccgag atctttctgt tgtacctctg
     4741 tatgtcatac caagagcatg tacactctct agttttatct ccgtaccacc cgcagggaca
     4801 gggattctga gcagcaacca tcatgaacct tgctggatat gtcgcagtta gctttgccct
     4861 ggccactgtt atcactccat cctctagcgg ctgtctgaga gcctcgataa catctcttct
     4921 gaattccggg aactcgtcca tgaacaaaac tccattatga gccagactaa tctcgcccgg
     4981 ctttgcgtca ttgcctcctc cgacaatgga cgttgtcgac gcagtatgat gcggcgatct
     5041 aaaaggcctc tcctttatta gtcctttctc tccaagaagg cccgcaacac tgtaaatcat
     5101 cgttgtctca atagcctctt ccatagtcaa aggaggaaga attgacggaa tcctgcgtgc
     5161 aagcattgtc ttgccgcatc ctggtgaacc tcgaagagag atgttgtgac cacctgcagc
     5221 agcaacttcg gaagctcttt tggcaaactc ctgacctttg atctcagaca tgtccggccc
     5281 actaacatta ccaagttctg gctgcgaata agcgatggga gacttctcta tatacccgtt
     5341 tagaaaaccg accagatcat ttagattatc ggccgtaaat attgtcaaat tgtctactat
     5401 tgaagcctcc tgtctgttag gagcaggtag cactatcttc ataccctttg cccttcttga
     5461 aagagaaagc aaaatcggca atactcccct tactcttctc aagcttccat caagacctag
     5521 ctctccaaag aagacaaagt ctgataggtc ggcgttaatc tttgaagtgg ctttgagaag
     5581 ccccacagct attggaagat caagataaga accctccttc ttcacatcgc taggagcgag
     5641 gttcacagtt atccttccat tcggaagctc aatcccagaa ttcttgacgg cactcttcac
     5701 cctcttcttg ctctccttaa taacggtgtc tcctagacct acaatatcca tttcctggat
     5761 gactgcccta ctgtcaatat ccagctcaac aagaacatcc agaactttca gaccaacagt
     5821 tgtaaccgtt ctaactcttg aatagcgcat tccatgacct cctatctcat ctcacattat
     5881 atcaatcaat gagcggagag gtgatatact taagttgagg tatggtcgag gtgtgcatat
     5941 gaagaataca catcgtatta aagtgagagc tctcattatt aaaaacgaat ctgtccttct
     6001 tgttcgtcat gagcatcacg acagacctcc cttctggtgt ttccctggcg gctttgtcga
     6061 atcggacgaa gatcttttca gtgcaatcaa gagagagata agggaagaga ctcaagtagt
     6121 ggtttcccca cgctctgtca ttgcacttca ggaattcaaa agagaaagcc ttctggaggt
     6181 gatattctcc tgcgattatg tctcaggaaa gttgaagctg ggatcagatc cagataatcc
     6241 gggaatccca actctggtcg atgcgaagtg ggtaagaatt gatgaacttg acaggttcaa
     6301 aatacttcca gttcaactcg cttctctttt aaagaacagc tggggtaatc tctgctcagt
     6361 agaactgttc cagatcgaaa ccatgagaga ggttccctag aaggaccatg atcccggtgc
     6421 actgtaacgg tctttgatat tgtgataaca tgtaataacg caacttgagt cacatcacgc
     6481 gagaggaggg ttatcatgga ttacaacatc tactctaaag acgtaactgt aacagactcc
     6541 atgaaagact acatcgacaa gaggttctcg aaagtgtcga aagttgtcaa cgaggagaag
     6601 ctaatctcga tggatctcag gctctcgaag gaaagagctt tttacaagat cgaagcgacc
     6661 gcccacctgc caggtgtcat ggttcgtgtc gaggagaagg gcagtgactt ctatacggtc
     6721 gttgattcat catcggatgc ctttgagaga aggattaaga gattcaagga gagaactcgc
     6781 tacaaacata aaaacggcct taaggaaaac ctcgattcaa ttccctccaa gtttgaggaa
     6841 gaccactcca caatgataac aagacgcaag aagttcacta ttaaacctat gaccgaggaa
     6901 gaggccgtgc ttcaaatgga gatgttaggc cacacttttt tcgtttacag aaacattgac
     6961 acagatgaag tgaatgttct ctataccaga aagaatggat ccataggact aattgaaatg
     7021 actgaatagc gaaaggccac ctgaaggtgg cccgcttttt tattatccga tgagatatac
     7081 tggttgtaga aaaactgaaa tctagttctg tctatagaaa tgaaagggcg gtggcaagaa
     7141 tacagaataa cagaacacag actatagtct tcagtcgaat cgaagacgag gtcaggattg
     7201 cttcgcttga cggtgatcaa ctcgaagaga tattctttga agatatggac agcgaaagtg
     7261 tcactggaaa tatctatctg ggtaaggtag agaatgttgt tcccagtctg gaagcggctt
     7321 ttgtaaatat tggaattgga agaaatgctt ttctaagatt caaggatgca tcgggacaac
     7381 caaaacttga aaagaacaac aaggttctcg tacaagtcag caaggatcct atcggctcga
     7441 agggacctca aataactctg aaagtcagca tccccggaag gagtttggtg tacactccct
     7501 tttcaaaaca tatcggaatc tctaagaaga ttacggacca gcaagaaaga caaagactta
     7561 ccgcagttgc gaagtccact ttagaaaaca aggaaggtgt gatattccgc acagccgctt
     7621 ttggtgttgg cgacgagaca ataaaagaag agcttgagag tctgcgtacc aactggaatc
     7681 agatctgcga gacgttcagg agaagcagaa aaccaaagat tctatacgag gaaccatcct
     7741 ttgtcgagta cattcttaga gagagactta ctgaaaacac gaaagaaata gtggttgact
     7801 cggaagagct ttggcacgac gtagtggccg gaatcagcaa gttcaaatcg ggcttcaaac
     7861 cagtcgtaag atatgtcgag ggcgatgcct tcgcaagcga agaagtgtac gagaagatga
     7921 agatccttta cacgagaatg gtttctctac cgggcggagg aaatatctac atagacagaa
     7981 ctgaagccat gacggtaatc gacgtcaatt cggcaagcaa tgtttccggc aacgacgttt
     8041 ccgaaacctc tctaaataca aacctggagg cagcttcaga aatagcaaga cagctcaaac
     8101 tccggaacat aggcggaata gttgtaatcg acttcattga catgaaaagc gatgaagatc
     8161 gtcaaaagat tatttccagt ctaacaggtg aattcaagaa agataaggcc aggacaatga
     8221 tcatgggatt cacgaggctg ggacttctag aaatgacaag gaagagatcg tctgcagcca
     8281 ttggatccaa gttatactct tcttgcccga tttgcaaggg aaccgggaga atcgagtctc
     8341 cgagagcagt ttatcagagg ttgcttaagg atctcgcgag aggctttgaa gatgaaacta
     8401 tcagctcggc aaccgttcaa gtttatcaga acatgtcggg aatccttacg ccagaaaacc
     8461 agaagaatct tgccaaatct ctgaagagag aaatctcggt gggctttacg tggcctattc
     8521 ctacctcata tgagatcaag ttcgcgaaga agcaagaaaa accaaagaga acacgaagga
     8581 aaacgaaaaa ggagtccaac tgactccctt atctattctg gtggatgcga cagggattga
     8641 acctgtggcc tcttccgtgt gaaggaagcg ctcttccgct gagctacgca tccgtatagt
     8701 agattctatc accggccaga tacagtgtca aggtttgaaa cctggcttcg tggtggcata
     8761 tggtggtgat ggcctgaaag aggggaagat cggtgttggg ggtcggaggt tggtcaggag
     8821 caaagaataa gagcttgaac cagtcctcaa gaagaaaatt acgcccgcgg atcagaacga
     8881 acatattttg tcatcct
//
LOCUS       DAOQYI010000058         8709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_8553_length_8709_cov_72.469841, whole genome shotgun sequence.
ACCESSION   DAOQYI010000058 DAOQYI010000000
VERSION     DAOQYI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8553_length_8709_cov_72.469841"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="PKL93_09765"
     CDS             <1..1829
                     /locus_tag="PKL93_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNQ71583.1"
                     /translation="LGLLMLSIAWWSLFYALELLSYDLESMRLLNKISYPGIVSVSVF
                     YFLFSMALVDKRDWFRWKRIVMLFIVPIAVLIAMWTNEQHWLFYSLSEIDHTSTFPLQ
                     RLVHGPLFWIAMLYNYLLLLSGIIAVFRKYIHSTGLYKGQLGTVLLGTVFPILVNINY
                     LFGFVDVGNLDLTPFAFTVAGVFAAAGVFRYRLFDIRPVARETVIERLPDGIVILDDG
                     GRIVDLNPMACKMLGIDASDAIGNSVQEVFSDIESVVSFFTSGSEKRELNVRERVIEI
                     KQSPIYSNKGKIRGHIALLTDITERKEVEDALRQSEERLALAVKGGKIGLWDLKMKKG
                     EFSINDRYYQIMGYSAGGSVITFQQWKEFVHPEDLLPCLEEMDKCYEGQTDYFDIEYR
                     MLSKGGEWIWVHDRGEAAERDEDGRATRILGTHIDISPTKQIEEALRESQMMYKKLAT
                     TDMLTGVMNRYSLTELLKLETERSRRYSAPLSLIMFDLDNLKTVNDNFGHLAGDDALK
                     LTCSFVQSNIRSCDYIGRWGGDEFLIVATNTDLEGAIKMAEKLRSGLSSVDKKEYGPL
                     TISIGVTTLLENESDFDGLLRRADEALYESKRSGKNRVTSVI"
     gene            2066..2944
                     /locus_tag="PKL93_09770"
     CDS             2066..2944
                     /locus_tag="PKL93_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ71584.1"
                     /translation="MRRGTREALTGWVTVLPALTVQVIFIYLPLAWAFYVSFFSWNMI
                     RPMRYVGLRNYERLFSSPDFWNSMGVTGIYILGTVIPSVIIGLLLAALLNIEWIKGRG
                     LFRTLFYIPVVTSMAAAAVIWGWLYEPNFGLVNYFLSIFGISNIKWLSDPNYALLALI
                     IVGVWKRIGYNMVLFLAGLQTIPKTYYEAAEIDGATPWRKFVNITLPLLSPTTLFVFI
                     MQFIASFRVFVSVSVMTSGGPAKSTQVITYYLYENAFKYLKFGYASAVAVVMFAMMVV
                     FTLIQFRVSKGRVHYQ"
     gene            2941..3771
                     /locus_tag="PKL93_09775"
     CDS             2941..3771
                     /locus_tag="PKL93_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ71585.1"
                     /translation="MRRKPALPIRIIVYLAILFVAIIINLPFFWMLVTSFKTEDAAFS
                     IPPSFLPTIFDLRNFVKAFELIPLARYIWNTLFVALTVTLLQLIFNSLAAYGLARIRF
                     RGANVVFMILIGTLMVPPEVTMVPLYVIVKQFGFIDKYSALIVPFMSSAFGIFLLRQF
                     FMGIPKELEEAAIIDGASRMKIFVRIILPLSKPALYTMALYTFLAHWNEYMWPLIVIN
                     DAKKQMIQVGISQFVSGWETQWTLRMAASTTAVIPIIILFFFVQKQFVEGISISGLKE
                     "
     gene            3800..5050
                     /locus_tag="PKL93_09780"
     CDS             3800..5050
                     /locus_tag="PKL93_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71586.1"
                     /translation="MKRTFVITLLVVAIAAIGFAAPVKVTMWYAQTGIYSQTLLDIAA
                     EFNKLHEGEIEVEAVYTGNYQDTMQKLLAAMVAGDLPTLAQIEQSRIGQFVDGGAFQD
                     LNYFIDKDPEFKATLDDFWPRFIQANTYEQGLLGFPLNCSTPLMYINRDLFRQAGLDP
                     DNPPKTWTEVYAASKQIKTLGEDIFGYRFGKDDWLLEAYIWQFGGEIISEDGRKMLIY
                     SPETVAAWNFFQKGVREGIFIFGVSGGNELDLSGRIAMVVRSTGSLGYLKQNAKYDLG
                     ATTMPGQVKEVVPIGGANVFMFSARPQAEKDAAWEFLKFLTSTENTKRWSLATGYMTS
                     RISAYESPEIQKIMTDDPRYALTYQQLRDCAVRRPWYGPYPEVHAMITSAWEDAMSNP
                     TKDVDAILKNLHEEAQDVLDDYYF"
     gene            5108..6898
                     /locus_tag="PKL93_09785"
     CDS             5108..6898
                     /locus_tag="PKL93_09785"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNQ71587.1"
                     /translation="MKKILIAVLVIVSSLSFGQVFFGNIHAHTSYSDGQGTPEEAFEH
                     ARNSGVVDIQAITDHDHDLNYPLPDGSWKLDRIIEMAEKYTVEGEFIAISGFEWTLTS
                     RGHITIYNTSEWIDRSTTDLYDIYQWIVDRKATAQFCHPGKKYGDFFDFEYFPEVDLY
                     INTIEVGNGAGANSNNVIKDEYFERYQRALNRGWHVGASANQDNHHKEWATANDARTA
                     FVIDELTTEKVSEALKTRNIYATEDRDAFISFSTEGAKMGDVLYDIPEATLKVEYSDP
                     GERTQRAYIYSNNGVMELNVSGDEWAIEIDVKNPLSYNWYFVKIDQIDGNEIVSSPIW
                     FQSSTNKYLLNARTIDPRPVKGLPFDVGFDLINSLREDQVISVEVRSGESVVFTKKYD
                     ISAMTCLEITETFDTVDKDLDFYVDDELAFRVNVDFVSFTAHLDTSHENYFVSELKPL
                     TDILMGLGGEVKPVIGELTEKTTFEGQVLILPLPDADTFMKDFMAISEEQIGFIVNYV
                     EKGGLLVVVLAKKEITHPSVESYKQLFEKLPWSVELENGGRSVSGTSTGNLEIGNGGG
                     VVILSWDEATGKSTIDEETMGFIELKLGLK"
     gene            6913..7662
                     /locus_tag="PKL93_09790"
     CDS             6913..7662
                     /locus_tag="PKL93_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ71588.1"
                     /translation="MSKLESFTLGEDGKRIFGICEYPRSGDSFPTVMMFHGFTGEHIV
                     STFKFPRLSRRLVEKGIATVRFDFRGSGDSEGEFYEMSPLTELRDAEEVHSFVRSRSW
                     CSGKVAIVGYSLGGMVASLFAGRHPEISSLVLWSPVIMNQEFFNREDYSFKDGEEYKD
                     VLGLKLGSIFFEDGRSVDASEELRNYEGDLLIVHGSDDESVPYLPVKKYANARRLKIH
                     TVEGANHKYQRIDWIEELFSVSTDFFERTLL"
     gene            complement(7670..>8709)
                     /locus_tag="PKL93_09795"
     CDS             complement(7670..>8709)
                     /locus_tag="PKL93_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71589.1"
                     /translation="FVLDKQKGPELLLSLFRLNNHEKMTPSDGESLEALSDWVFKARL
                     SLTEESLTSLARLFSWEAFFGLKIIPYFFLEGSPTPSGLLKMIETISPERLLSLFLSS
                     DFMPLEKRNDVFLKSLEENEKGALEYVSSIPWLLSSDRYEAFQMLTDQAKTHESFLEL
                     LRFAEKEIASMVELENLVNYGEEILLENIERFESGFLAFLLDIEPPSLSGKMIRLHVS
                     AFLGGSVITIEVPEKNSVISLIGVDRVRKRSLSYEKRSAAGVISAMSGQNSLRILKIV
                     MRSSQCIDEIAFLSGVHRHEVIEILAALSRQGLVIPEIHEKELTFTSEERLIDCALEE
                     IRKAIKEGAGD"
BASE COUNT         2319 a         1842 c         2193 g         2355 t
ORIGIN      
        1 ctcttggcct attgatgctg tcaatagcgt ggtggtcact tttctatgcg ctggagctgc
       61 tttcttatga tctcgaaagt atgaggcttc tgaacaagat ttcttatcca ggaatagtgt
      121 ccgtttcggt cttttacttc ctcttttcca tggcacttgt ggacaaaaga gattggtttc
      181 gttggaagag gattgtgatg ctatttatcg tacccattgc tgttttgatt gcgatgtgga
      241 caaatgaaca acactggcta ttttactcgc ttagtgagat agatcacact tcaactttcc
      301 cccttcagag attggtgcac ggtccgctct tctggattgc aatgttgtac aattacttac
      361 tgcttttatc aggaatcatt gctgtcttca gaaagtatat ccattcgacg ggtctatata
      421 agggtcagtt agggactgtt ctactgggaa cggtattccc catcttggtc aacattaact
      481 atttgtttgg tttcgttgat gtggggaatt tggacttgac tccttttgcc tttacagtgg
      541 ccggcgtatt tgccgctgca ggagttttca gatataggtt gttcgacata cgtcccgtag
      601 ccagagagac cgtaatagaa aggcttccag acgggatagt cattcttgac gacggtggaa
      661 gaatagttga tcttaatccg atggcttgca aaatgctggg cattgatgct tcagatgcca
      721 ttggaaattc agtccaggag gtcttcagcg atatcgagtc tgttgtaagt ttcttcactt
      781 ccggctccga aaagagggag ttgaacgtgc gagagagagt catagagata aagcagagtc
      841 ccatctacag caacaagggc aagatcagag ggcatattgc gctgttgaca gatataaccg
      901 aaagaaaaga ggtcgaagat gcgctccgcc aaagtgaaga gaggctcgca cttgcagtga
      961 aaggcgggaa gataggtctc tgggacttga aaatgaagaa aggcgagttt tcgataaacg
     1021 accgatacta tcagatcatg ggctattcgg caggcggatc tgtcattacc tttcaacaat
     1081 ggaaggaatt cgtacaccct gaggaccttc ttccctgcct tgaggaaatg gataagtgct
     1141 acgaaggtca gactgactac ttcgacatcg aatacaggat gctttcgaag ggtggagagt
     1201 ggatctgggt tcatgatcgt ggcgaagcgg ccgaaagaga tgaggatgga agggcgacca
     1261 ggattctcgg aactcacatc gacatttctc cgacgaagca aattgaagaa gcgcttcgcg
     1321 agagccagat gatgtacaag aagctggcca ctacggatat gctaaccggg gtgatgaaca
     1381 ggtattcgct aaccgagctt ctcaaactgg aaacggaaag gtctagaaga tattctgctc
     1441 ctctatcgct gataatgttc gatcttgaca acctgaagac cgtaaacgat aattttggcc
     1501 atctcgccgg tgacgatgcg ctgaagctga catgttcgtt cgttcagtca aatatcagga
     1561 gctgtgacta tatcggtagg tggggaggcg atgagttctt gattgtcgca acgaacaccg
     1621 atctcgaggg cgcaattaag atggccgaaa agctcagatc ggggctcagt tctgttgata
     1681 aaaaagaata tggacctttg accattagca ttggtgttac gacgcttctg gaaaatgaaa
     1741 gcgattttga cggtttgctt cgaagagcgg atgaggcact atatgaatca aagaggagtg
     1801 gaaagaacag agttacgtct gtgatttgac aattagaatg gagatttttg aagtggtttc
     1861 gaacctgaaa gcagtaagaa tcgaagagtt gattttgctt ttccaattat gctagactat
     1921 ccctagcgtg acagagatga tgagaaagtc gtgccagcta taaggcggcg actttttttg
     1981 ttttgcttga tcttccttta acgtattttg gtcataatgg ttgtgttata gattctttca
     2041 tatttacaac tgtggggtga tacgattgcg tcgcggaacc agggaagcgc ttaccggctg
     2101 ggtgacagtc ttgccagctt tgactgttca ggtgatcttt atctatttgc cactagcgtg
     2161 ggctttctat gtatctttct ttagttggaa catgataaga ccgatgaggt acgttggact
     2221 ccgtaattac gaaaggctct tcagctcacc ggatttctgg aattcgatgg gggttaccgg
     2281 gatatatatc ctcggcaccg taatcccttc ggttattatc ggactgttgc ttgctgctct
     2341 gctcaatatt gagtggataa agggaagagg gcttttcagg acgctcttct acattccggt
     2401 tgtcacttcg atggccgccg cggccgttat ttggggctgg ttatatgaac cgaatttcgg
     2461 actggtaaac tacttccttt ccatttttgg tattagtaac ataaagtggt taagcgatcc
     2521 gaattacgca ctccttgcgt tgatcatcgt tggtgtctgg aaacgtatcg gctacaacat
     2581 ggtgctcttc ctggcgggtt tacagactat cccgaagaca tactacgagg cggccgaaat
     2641 cgacggggcg acaccttgga ggaagtttgt aaatattacg cttccgctcc tatctccgac
     2701 gactctcttt gtgttcatca tgcaattcat tgcatcgttc agggtcttcg tctcggtatc
     2761 cgttatgact agcggtgggc cggcgaagag cactcaggtt atcacatact acctttatga
     2821 aaatgccttc aagtacctta agtttggata cgcgtcagca gttgccgtcg tcatgtttgc
     2881 gatgatggta gtattcactc tgatacaatt cagagtaagc aaagggaggg tgcattatca
     2941 atgagaagaa aaccggccct tccaattaga atcatcgttt acctggcaat acttttcgtg
     3001 gcgataatta tcaatcttcc ttttttctgg atgctcgtta cttcctttaa gacagaggat
     3061 gctgccttct ctattccacc gagctttctg ccgactatct ttgatttaag gaattttgtc
     3121 aaagccttcg agctgatacc gcttgccaga tatatatgga atacgctttt cgtagcgctt
     3181 acggtgactt tgctacagtt gatcttcaat tctctcgcag cttatgggct ggcgagaata
     3241 agattcagag gagctaatgt ggtcttcatg atcctgatcg ggacgcttat ggttccgccg
     3301 gaagtcacta tggtccctct gtatgtaatc gtcaagcaat ttgggttcat agataagtat
     3361 agtgctctaa tagttccatt catgagttct gcattcggaa tattcctgct acgtcagttc
     3421 ttcatgggaa ttccaaaaga gcttgaggaa gctgcaatta ttgacggggc cagcagaatg
     3481 aagatcttcg tcaggatcat tctgccactt tcgaaacctg cgctttatac tatggcgctg
     3541 tacactttcc tcgcccactg gaatgaatat atgtggccgc tgatcgttat aaatgatgca
     3601 aagaaacaga tgattcaggt cggtatatct cagtttgtaa gcggctggga gactcaatgg
     3661 acgttaagaa tggccgcttc tacgacggcg gtaattccaa taatcatctt gttcttcttt
     3721 gttcaaaaac agtttgtaga aggaattagc atttcagggt tgaaagagtg acccaaatct
     3781 cgaaagggga ggtttttttg tgaaaagaac atttgtaatc acgctactgg tagttgctat
     3841 cgctgcaata ggttttgcgg cgccggtaaa ggttaccatg tggtacgcac agactggtat
     3901 ctattcccaa acactgctcg atattgctgc ggagttcaac aagttgcatg agggagaaat
     3961 cgaagtagag gcagtctaca ccgggaatta ccaggataca atgcagaagc tccttgccgc
     4021 tatggtagcc ggcgacttac ctactctggc tcagattgag cagtcgagga tcgggcagtt
     4081 tgtcgatgga ggcgctttcc aggacctcaa ttacttcatt gacaaggatc ccgagttcaa
     4141 ggcaacgctc gacgacttct ggccgagatt cattcaggcc aatacgtatg aacagggtct
     4201 gcttggattt cctctcaact gcagcacccc gttgatgtac atcaacaggg atcttttcag
     4261 gcaggccgga cttgatcccg acaatcctcc gaagacctgg accgaggtct atgctgcctc
     4321 gaagcagatc aagactcttg gtgaagacat tttcggttac agattcggga aagatgactg
     4381 gctcctggaa gcttatatct ggcagtttgg aggagagata atctccgagg acggcagaaa
     4441 gatgcttatc tactccccgg aaactgtggc cgcctggaac ttctttcaga agggtgttag
     4501 agaaggaata tttatattcg gagtcagtgg cggaaacgaa ctcgatctct ccggaagaat
     4561 tgcaatggtc gtccgttcca ccgggagtct tgggtatttg aagcagaacg cgaagtacga
     4621 tctcggcgca actaccatgc ccggtcaggt aaaggaagtt gttccgatag gtggagcaaa
     4681 tgtcttcatg ttctccgcaa gacctcaggc cgagaaggat gcagcatggg agttcttgaa
     4741 gttcctcact tccacagaga atacgaagag atggtccctc gcaaccggtt acatgacgag
     4801 caggatttct gcgtacgaat ctcccgagat tcagaagatc atgacagatg acccgagata
     4861 tgctcttact tatcagcagc tgagagactg tgctgtaagg agaccatggt acgggccata
     4921 tccagaagtt catgcaatga tcacttctgc ctgggaagat gcaatgtcta acccaacaaa
     4981 agacgttgat gcaattctga agaatttaca tgaagaagca caagatgtgc tcgatgatta
     5041 ctacttttga tcgatagcat gacgcacggg ggcagtcatt ggctgcccct tttttagagg
     5101 tgattttatg aaaaaaattc tgatagcggt tctcgtaatt gtttcatctc tctctttcgg
     5161 tcaggttttc tttggaaaca ttcacgccca cacatcttat tcggatgggc agggaactcc
     5221 tgaggaggcc tttgaacacg caagaaattc gggtgtcgtt gacatacagg caataacaga
     5281 tcatgatcac gatctgaact accccctgcc tgacggctct tggaagctcg acaggattat
     5341 cgagatggct gagaaataca cggtagaagg tgaattcatt gccatatccg gcttcgagtg
     5401 gacgctgacc agtcggggac acataacgat atacaatacc tctgaatgga tcgacaggag
     5461 cactactgat ctttacgata tttaccagtg gattgtcgac aggaaagcga ccgcccagtt
     5521 ctgtcatccg gggaagaagt acggtgattt ctttgacttc gagtacttcc ctgaggtaga
     5581 tctttacatc aatacaattg aggtcggaaa cggagcggga gctaactcca acaatgtcat
     5641 aaaagatgag tacttcgagc gttaccagcg agcgctgaac agaggctggc atgtaggcgc
     5701 ctctgcgaat caggacaatc atcataagga gtgggccaca gcaaatgatg caaggaccgc
     5761 cttcgtaatt gatgaactta caacggaaaa ggtctctgaa gcgctgaaga caaggaacat
     5821 ctacgcgaca gaagatagag atgctttcat ctcgttctcc actgaagggg cgaagatggg
     5881 agacgtcctt tatgacattc cagaagcaac cttaaaagtg gaatacagtg acccgggaga
     5941 aagaactcaa cgggcgtata tatacagcaa caacggagtg atggagctga atgtttccgg
     6001 ggacgagtgg gctatcgaaa tagacgttaa gaaccctctg tcgtataact ggtacttcgt
     6061 aaagatcgat cagattgacg gtaacgagat agtatcgtca ccgatctggt ttcaaagcag
     6121 tactaacaag tatctcttaa atgcaagaac aattgatccc aggccggtaa agggcctgcc
     6181 ttttgatgtg ggcttcgatc tgataaattc cttgcgggaa gaccaggtaa tctctgtcga
     6241 agtccgaagt ggcgagagtg ttgtcttcac gaaaaagtac gatatttccg ctatgacctg
     6301 tcttgaaata accgagacct tcgacactgt tgataaggac cttgacttct atgtcgacga
     6361 tgagcttgct ttcagagtca acgtcgattt tgtaagcttc acagctcatc tagatacctc
     6421 tcacgagaac tactttgtct ctgagctgaa acctctaacg gacatcctta tgggtctcgg
     6481 cggggaagtg aaaccggtga tcggagagct cactgagaag actacttttg aagggcaggt
     6541 cttgatactt ccgcttccgg atgcagatac ctttatgaaa gacttcatgg cgatttcaga
     6601 agagcagatt ggcttcatcg tgaattatgt cgagaaaggt ggtctacttg ttgttgttct
     6661 tgcaaaaaaa gagatcactc acccatctgt agagagttac aagcagctct ttgagaaact
     6721 tccctggtcg gtcgaacttg agaacggcgg aaggtcggtg tctgggacga gcacaggaaa
     6781 ccttgaaatc gggaacggcg gcggagtcgt aatactcagt tgggacgaag cgactggaaa
     6841 gtctacaata gatgaagaaa caatgggctt cattgaatta aaattgggtt tgaaataaag
     6901 cgagggatag atatgtcaaa attggagagc ttcacactag gggaggatgg aaagaggata
     6961 tttgggatct gcgaatatcc aagatccgga gatagctttc ccacagtaat gatgttccac
     7021 ggattcacgg gagaacacat cgtttccacg ttcaaatttc cccgtctctc gagaagactc
     7081 gtcgagaagg gaatagcgac tgtgaggttt gatttcaggg gttccggaga tagcgagggt
     7141 gaattctacg agatgtcacc tttgactgag cttcgcgacg cagaagaagt ccattccttt
     7201 gtcagatcga gaagctggtg ttcgggaaag gttgccattg tcgggtacag cctgggtggt
     7261 atggttgctt cgctttttgc cgggagacat cccgagatta gttcactggt tctgtggtcg
     7321 cctgtaataa tgaatcagga gttcttcaac cgcgaggatt actccttcaa agatggcgag
     7381 gagtataagg acgttctggg gctgaagctg ggatcgatct tcttcgaaga cggtcgcagt
     7441 gtcgatgctt cggaggaact cagaaactat gaaggagatc tgctaattgt tcatggttcc
     7501 gacgatgaat cggtgccata tcttcccgta aaaaagtatg caaatgcgag acggttgaag
     7561 atacatactg tagagggagc caaccacaag tatcagagga ttgactggat tgaggaactc
     7621 ttctcagttt caacggattt ctttgaaaga acattgctgt agcgggcgat caatcgcccg
     7681 ctccttcctt aattgccttt ctgatttctt ctagagcaca gtcgataagc ctttcttcgg
     7741 aagtgaaagt cagctctttt tcatggattt ctggaatcac tagaccttgc ctgctcaacg
     7801 ccgcaaggat ttcaatgacc tcatggcgat gaactccgct caaaaaggca atttcatcga
     7861 tacattgact tgaccgcatg acaattttaa gaatacgaag cgaattctgg ccagacatcg
     7921 cactaataac tcccgcagcg ctccttttct catatgaaag actccgcttc ctaactctgt
     7981 ctactccaat gagtgatatg actgagtttt tctcaggaac ttcaatcgtg atcactgagc
     8041 ctccaagaaa cgccgaaacg tggagcctga tcatcttacc agacagagat ggaggttcaa
     8101 tatccagtag gaaggctaag aacccgcttt caaatctctc gatgttctca aggagaatct
     8161 cttccccata gttgacaaga ttctccagtt caaccattga agctatctcc ttctcggcaa
     8221 aacgaagcag ttcaagaaag gattcatgtg tcttggcctg gtccgttagc atttgaaacg
     8281 cttcatatct gtcagaagat aagagccagg gaattgacga aacatactca agagcgccct
     8341 tttcattttc ctctaaagat tttaaaaaaa catcattcct cttttcaagt ggcatgaaat
     8401 cgctgctaag aaacagcgaa aggagccttt cgggagatat cgtttctatc atcttcagta
     8461 gacctgacgg agtaggggaa ccttcgagaa agaagtaagg gataatcttc aaaccaaaga
     8521 aggcctccca gctgaaaagt cttgcgagtg atgttagact ctcctccgtg agagaaagcc
     8581 ttgccttgaa aacccagtca gagagggctt ctagggactc tccgtcgctt ggagtcatct
     8641 tttcatggtt attcagtctg aacaaggata gaaggagctc aggtcccttc tgcttatcaa
     8701 ggacaaaat
//
LOCUS       DAOQYI010000059         8612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_8679_length_8612_cov_74.082389, whole genome shotgun sequence.
ACCESSION   DAOQYI010000059 DAOQYI010000000
VERSION     DAOQYI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8679_length_8612_cov_74.082389"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1328
                     /locus_tag="PKL93_09800"
     CDS             33..1328
                     /locus_tag="PKL93_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71590.1"
                     /translation="MKKFLVLVLLCLSIFAFGKTTINFWHAMSGSRLGAVEAIVEGFN
                     AENPDIEVVAQFTGSYAETLTKAIASYRAGDQPHIVQVYEVGLQTMLDSGAIKPVFEL
                     SGPDYDWGDVIGPILDYYTVGDRLYSMPFNSSSAILYYNKDIFRAAGLDPSEPPSTFE
                     EVYEMGKKIVESGAAQGGISFGWPAWVFEQMHSVHGQFYANNENGRAAKATEVFFNDD
                     FGVKVLAEWIKWAQAKVFLYGGREYDANQAFLSKQVAMLIQSTSSVSSIESKADFEMG
                     TTFLPVMPGYPKGNSVIGGATLWVMKGHTDEEYEAIWKFFQYLMRTEVTAEWHKSTGY
                     FPTTNSAVKKLMDQGWFSENPNHLTAFLQILSGTRIPEAQGVRLGNFVAIRDIVDGAI
                     ENAIQYTGTDYEGEAKRILDDAAEKANTVLEEYTLIYGK"
     gene            1392..2273
                     /locus_tag="PKL93_09805"
     CDS             1392..2273
                     /locus_tag="PKL93_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ71591.1"
                     /translation="MNSRFPNKLLPYLLLLPSVVVVIVFLIIPTIQSLYLSFFRVSPF
                     GDRLIFVGWYNFTKLFKSSDYVDSLMITLVFAVFVVIIGLTVGMLLSVLLNQKLRGLQ
                     FYRTLFIWTYAISPAVAGTIWALMFSPSSGPVTYLLKTFFGAEVNWMMDGRVALIAVI
                     IAASWKMLGYNIIFFLSGLQTIPQELTEAAAIDGASSVRRFFRITLPLLSPTTFFLLI
                     MNTLYAFFQVFGLIDIMTKGGPGDATQVLVYKLYRDGFINLDTGFASAQSIVLFVFVA
                     ILTVLQFRFAERKVFYG"
     gene            2290..3120
                     /locus_tag="PKL93_09810"
     CDS             2290..3120
                     /locus_tag="PKL93_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ71592.1"
                     /translation="MKQRKSSVFLSYLILTVAIVAIMFPIYYAFTMSTFNDKEAYSFP
                     PKFLPSVHAFDNYVKAWKTVNMGRLIFNSAFIAVTVALAKIFLSMLAAFAFTYFGNFR
                     GKFFFFAVILITHMLPLPIRIVPTYELMRTFGWVDTYWALTIPFFASATGTLLFRQLF
                     MTVPSSLSDAARIDGAGPMRFLFSVLIPLSKTNVGALFLIEFTYMWNEYLWPMIVTNS
                     NNMRVVQIGIKMLLASEAQAAEWNIIMAGAIIAMIPPLIMLLLFQKTFMEGFSLKDEK
                     "
     gene            complement(3136..3537)
                     /locus_tag="PKL93_09815"
     CDS             complement(3136..3537)
                     /locus_tag="PKL93_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNQ71593.1"
                     /translation="MKRRILIRILLVAGIVVVSLIVIIMVFVVPKLEADLKKLDNLEF
                     EQLNLSTLKDGIYEGSFEAGIVSASVRVEVSGHEVKSVEIVKHNHGRGKAAEAITNSV
                     VEKQSVQIDVVSGATYSSKVILKAIENAINR"
     gene            complement(3589..4050)
                     /locus_tag="PKL93_09820"
     CDS             complement(3589..4050)
                     /locus_tag="PKL93_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNQ71594.1"
                     /translation="MVYATRSGTSEKCSIILSEKLSGEKTIIDIKRNPSPDLTKFDAV
                     IVGASIRIGKVQRTISEFVQRNLATLIKKRLGVFLCMGSGEENFSEYLSQNFPESFLN
                     NCKAKGFFGGEFNLERLGFLSRMMVRTAFKGKPQPHVVSSNIEKFVKEFEN"
     gene            4296..4769
                     /gene="rpsF"
                     /locus_tag="PKL93_09825"
     CDS             4296..4769
                     /gene="rpsF"
                     /locus_tag="PKL93_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNQ71595.1"
                     /translation="MQRIYETMFIVSPKLDEENRNATAEKVRDYIVERVGGTVEKFDR
                     WGMRKLAYRVSKGFSEGDYTVIQFKADPESVDTLERFFGVTPEVFRWQTFRREDLEKA
                     EKKALLKQEEAVDETQAPDENLDDEAVLENSEETATDGAVDVESEKIEEVKQTED"
     gene            4773..5234
                     /gene="ssb"
                     /locus_tag="PKL93_09830"
     CDS             4773..5234
                     /gene="ssb"
                     /locus_tag="PKL93_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNQ71596.1"
                     /translation="MSISYNHVVLIGRLTRDPEIKFAASGTQIATFTLAVDRNVPSSN
                     NDNTDFIRIVTFGKTAEFAGNYLTKGRLILVEGSLRINKWKTQDGEPRSNAEVAASNI
                     RFMETKAQAQQSSGGFDRSIQDTNVVESTENDDITFFGSETEDSGSDDIPF"
     gene            5250..5483
                     /gene="rpsR"
                     /locus_tag="PKL93_09835"
     CDS             5250..5483
                     /gene="rpsR"
                     /locus_tag="PKL93_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNQ71597.1"
                     /translation="MVRDNRRRGRSRRKCRLCGSKTTYIDYKNISLLRDYVTEKGKII
                     PKRITGNCAKHQRMVKEAIQRARFMALLPYTKE"
     gene            5533..5988
                     /gene="rplI"
                     /locus_tag="PKL93_09840"
     CDS             5533..5988
                     /gene="rplI"
                     /locus_tag="PKL93_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNQ71598.1"
                     /translation="MKVILLKDVSNIGKTGEVKNVSDGYGRNFLIPKGFAVEANAKEL
                     AKLKQIQKQKEEKEERIRKSSEDLLHELQKHHFIIKAKSGTSGKLFGAVTSGDIANHI
                     RNSLGVEIDKKEIDLGDHIKQTGEYRIDIKLPGNVKGKIALKVEGLEEE"
     gene            5990..6841
                     /locus_tag="PKL93_09845"
     CDS             5990..6841
                     /locus_tag="PKL93_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ71599.1"
                     /translation="MEFQIFSKALYSTWILYRPERILFDVGEGISTILGNSVYAIKDI
                     FLTHGHVDHISGLWGLINTRNTAMGDRNKQLRINFPSGNRAIEAYLDFIIGMNPRLRY
                     NMIINPLNIGDEVFLRTAGSFRRHVTPFRVKHTIGEISYGYHIYEERRKLKEAYSGLT
                     SKEIARVVKEKGREEITDTYLKKIVTISGDTFALSPETVSYSETLLHECTFLVSKDRR
                     NQNHSSIDEVIDTVRRSRGIKRLILYHISGRYNSQIKKWHNIIKKELEDTEIDVFLVH
                     PEHVFEL"
     gene            6875..7318
                     /locus_tag="PKL93_09850"
     CDS             6875..7318
                     /locus_tag="PKL93_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNQ71600.1"
                     /translation="MGYIGWMIFGIILLVAEILTPTFFSLWFSIGSFLAGLAAMFSLG
                     LGWQVLIFASSSTFLVLLSRPIAKKLSKGDSPKKMYIDGMVGSLGRVTVEINPVMGKG
                     MVRIDGEDWRATSANGEIIPVDTVVKIVRLEGTLVYVERQNNNNQ"
     gene            7340..8272
                     /locus_tag="PKL93_09855"
     CDS             7340..8272
                     /locus_tag="PKL93_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNQ71601.1"
                     /translation="MVFWLILAVVIFIIAASGIKIIRPFEKGLVERLGKYRRDAEPGL
                     QFIIPFMERMVKVDLRETVIDVPPQEVITKDNVVVTVDAIIYYQITDAFRVVYNVSNF
                     EIAAIKLAQTNLRNVIGEMELDQTLTSRERINVTLREVLDEATDKWGVKVTRVEIKKI
                     DPPQDIMDAMSKQMKAERTKRAVILEAEGYKQSEITKAEGDKMSAILQAEGQSESIKR
                     VAEANKFKLVTEAEGEANAIINVFKAIHEGDPTRDVIAIRYLDALKQIADGKANKVFL
                     PYESSAMLSSLGSMAEIFKDGKDLSGGNEKVSKK"
     gene            8278..>8612
                     /locus_tag="PKL93_09860"
     CDS             8278..>8612
                     /locus_tag="PKL93_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71602.1"
                     /translation="MLISETLRIFFSTLLIAGLATVLSVASIFAFQRQIKKANFLLLF
                     MNVAFASGVLFLCNIVFVRENRLHNVILFLISYLYIYGSLGRHTSRYDDTFRMLFLSM
                     GYTRQEFFK"
BASE COUNT         2476 a         1708 c         2059 g         2369 t
ORIGIN      
        1 ccgttttcaa tttttgaaaa gggaggggta ttatgaaaaa gtttcttgtg ttagttcttc
       61 tgtgtctatc gatctttgct ttcggaaaaa ccacgatcaa cttctggcac gctatgagtg
      121 gtagcagact gggagcggtc gaagcaattg tcgaaggttt caatgccgaa aatcccgaca
      181 ttgaagtggt ggctcagttc acaggttcat acgcagagac acttaccaag gccatagctt
      241 cgtaccgagc gggagatcaa cctcacattg tccaggtcta cgaagtgggc cttcagacta
      301 tgctggacag tggcgcgatc aaaccggtct ttgagctctc tggcccggac tatgattggg
      361 gagacgtaat tggaccaatt cttgactatt acactgttgg tgatcgactt tactctatgc
      421 ccttcaattc ctcaagcgcg atcctctatt acaacaagga tatcttcaga gctgcaggac
      481 ttgatccaag cgaacctcct tctacttttg aagaggtcta cgagatggga aaaaagattg
      541 tcgaatcggg agctgctcag ggaggaatat ctttcggctg gccagcgtgg gtattcgagc
      601 agatgcattc cgttcacggg cagttctacg caaacaacga aaacggtaga gcggccaagg
      661 caactgaggt atttttcaat gatgattttg gtgtcaaggt tcttgcagaa tggatcaaat
      721 gggctcaggc aaaggtattc ctttatgggg gccgggagta tgacgccaat caggccttct
      781 tgagcaaaca ggttgcaatg ttgattcagt cgacttcgtc tgtgagttcg atcgagtcaa
      841 aggccgattt tgaaatggga acaaccttcc tgccggttat gccaggctat ccaaagggaa
      901 actctgtcat tggaggagcg accctgtggg taatgaaggg ccatacagac gaagagtatg
      961 aggcgatctg gaaattcttc cagtacctca tgagaacaga agttaccgca gagtggcaca
     1021 aatctaccgg ctatttcccg acaactaaca gcgcagtgaa gaaactcatg gatcaaggct
     1081 ggttctcgga aaatccaaat caccttaccg ccttccttca gatactttct ggaaccagaa
     1141 ttccggaagc acagggagta aggctaggaa atttcgttgc aataagagac attgtcgacg
     1201 gtgctatcga aaacgcaatt cagtacacag gaactgacta cgagggagag gcaaaaagaa
     1261 ttctcgatga cgctgccgaa aaagcaaaca ctgttcttga agaatacaca ttgatttacg
     1321 gtaagtgaag attaagatcc cctgacagat ttgtcagggg atccttagta ttttccggat
     1381 ggtgattaag catgaacagt cgtttcccca acaagctatt gccgtacttg ttacttctac
     1441 cttcggtagt cgtggtaatt gttttcttga ttatccctac tattcagtct ctttacttga
     1501 gttttttcag agtttcccca tttggagaca ggctaatctt tgtgggttgg tacaatttca
     1561 cgaaattgtt caagtcttcc gactatgttg acagtctgat gattaccctt gtcttcgcgg
     1621 tcttcgtagt gataattggt ttgactgtgg gaatgctcct ttcagtactt ctaaaccaga
     1681 aactaagggg actacagttc tacagaaccc tttttatttg gacgtacgcg atttcgccgg
     1741 ccgttgcggg aacgatttgg gcactgatgt tcagtccttc gagcgggccg gtaacatatt
     1801 tactaaagac gttttttgga gccgaagtca actggatgat ggacggaaga gtcgctctga
     1861 ttgcagtaat tatcgctgcc tcctggaaaa tgcttggcta caacattata ttctttttgt
     1921 cgggtcttca gacaattcct caagagctta ctgaagcagc tgcgatagat ggtgcatcca
     1981 gcgtacgcag attttttaga attacgttac ctctgctgtc cccgacaaca ttctttcttc
     2041 tgattatgaa tacactttac gcattcttcc aggtctttgg gctgatagac ataatgacta
     2101 aaggaggacc cggcgatgct acacaggttc tagtttataa gctgtacaga gatggtttca
     2161 ttaacctgga tacgggcttt gcttctgctc aatcgatagt tctttttgtt tttgtggcga
     2221 ttttgaccgt tttgcagttc agatttgctg agcgcaaggt cttctatgga tgatggtggg
     2281 gtggactaga tgaagcaaag aaagtcttca gtattcctga gctacctaat attgacagta
     2341 gcaatagtag caataatgtt tccaatatac tatgcgttca ccatgagcac tttcaatgat
     2401 aaggaagcct actcgttccc cccgaagttt cttcccagcg ttcatgcttt cgataactat
     2461 gttaaagcgt ggaaaacagt caacatgggg aggttaatat ttaacagcgc ctttattgca
     2521 gtcactgtgg cgcttgccaa gatctttctt tctatgcttg cagctttcgc tttcacgtat
     2581 ttcggcaact tcaggggcaa gttctttttc ttcgcagtca tactcataac acacatgctg
     2641 ccgctaccaa tcagaatcgt gcctacttat gagcttatga ggacatttgg ctgggttgac
     2701 acttattggg ctttaacaat ccccttcttt gcaagtgcga caggaacgct tcttttcagg
     2761 cagctgttca tgacggttcc ttcttctctt tccgacgcag ccagaattga cggggcggga
     2821 ccaatgagat tcttgttcag cgtattgatt ccactatcga agacgaacgt cggagcactt
     2881 tttctcattg agttcaccta tatgtggaat gaatacttat ggccgatgat cgttaccaac
     2941 agcaataata tgcgtgtagt tcagatagga atcaaaatgc tgttggccag cgaagcgcaa
     3001 gctgcagaat ggaacataat aatggcggga gcaataatcg caatgatacc ccctctaatt
     3061 atgttgctac tcttccagaa gaccttcatg gaaggattca gcctaaaaga cgagaagtag
     3121 aagtttataa aggttctacc tgttgatagc gttttcaatc gctttcagaa tcactttgct
     3181 gctataggta gcacctgaaa ctacatcgat ctgaaccgat tgtttttcta caaccgagtt
     3241 agtgattgct tctgcagcct tccctcgacc gtggttatgc tttactatct caactgactt
     3301 gacctcatgc ccggaaacct ctactctcac agatgcgctt acgattcccg cttcaaaact
     3361 gccctcgtag attccgtctt tcaatgtaga tagatttagc tgctcgaatt caagattgtc
     3421 aagtttcttt agatctgctt ctagttttgg aacgacaaag accattatta ttactattaa
     3481 ggaaacaaca acaattcctg ctaccaagag tattctgatc aatatcctac gcttcatctc
     3541 ttccccctga aattgaattc tttcgatatc ctaaaagaaa tcggatttct agttctcgaa
     3601 ctccttcaca aatttctcaa tattgcttga aacgacatga ggctgaggtt tgcctttgaa
     3661 agcggttctt accatcatcc ttgaaagaaa gcccagtctc tcaaggttaa actcaccccc
     3721 gaaaaagccc tttgctttgc agttatttag aaaactctcg ggaaagtttt gagaaaggta
     3781 ctcgctgaag ttttcttctc cagaccccat gcacaaaaag acgcctaacc gtttctttat
     3841 tagtgtggca agatttctct gaacgaattc ggagatagtc ctttgaacct tacctattct
     3901 gattgaggct ccgacaatta cggcatcgaa tttcgtgagg tctgggcttg ggtttctctt
     3961 aatgtcgata atcgtctttt cacctgaaag cttttctgag agaataatgg aacacttctc
     4021 ggacgtaccg cttctagttg catagactat tgccgtgata aagactcctc ccttgttgat
     4081 agtgcggtcc agttttcttt caatttagtt tacattattt gcagctctag agattgcata
     4141 tgcgaaaagc aagcatttgc gattcgtttc cgaggaggaa cgaattgtta gacagcgcgc
     4201 tcttgaagaa ttctcgcatt gtgttataat tctatgcgtt gaaatcccgc tttgcttaag
     4261 aaggcaaagc cacagtccaa gggaggagga agttaatgca gaggatttac gaaacgatgt
     4321 tcattgtctc tccgaagctt gatgaagaga atcgtaatgc gacggctgag aaggtgagag
     4381 actacattgt cgaacgagtt ggaggaacag ttgagaagtt cgacagatgg ggtatgagga
     4441 aactcgctta cagagtttca aagggtttca gtgaaggtga ttacacagtt atccagttca
     4501 aagccgatcc cgaatcagta gacacgcttg agaggttctt cggggttacg cctgaggtct
     4561 tcagatggca gacgttcaga agagaggacc ttgaaaaggc tgagaagaaa gcgctcttga
     4621 agcaggaaga ggcagtcgat gaaactcaag ccccagacga aaatcttgac gatgaagcag
     4681 ttctcgagaa ttctgaagaa actgctactg atggagcagt cgatgtagaa tctgaaaaga
     4741 tagaagaagt aaagcagacg gaggactaag agatgtcaat tagctacaat catgttgtcc
     4801 tgattggcag actcactcgt gatccagaga tcaagtttgc tgccagtggt actcaaatag
     4861 cgacgtttac acttgcggtc gacagaaacg taccttcatc caacaacgac aataccgatt
     4921 ttataaggat tgtgacattt ggaaagacgg ccgagtttgc cggaaactat ttaactaaag
     4981 gtcggctaat acttgtcgag ggttcactaa gaatcaacaa atggaaaacg caggatggtg
     5041 aaccaagatc aaatgccgaa gtcgccgcct cgaatattag attcatggaa accaaagctc
     5101 aggcccaaca gtcttcgggc ggtttcgata gatcgattca ggatactaat gtggttgaaa
     5161 gcactgaaaa cgatgacatt actttctttg gaagtgaaac tgaagattcg gggagcgacg
     5221 atatcccgtt ctaaatcagg aggaatatta tggtaagaga caataggcga agaggaagaa
     5281 gcagaagaaa gtgcagactc tgtggttcaa agacaactta cattgattat aagaatatct
     5341 ccttgctacg cgattatgtt actgaaaagg gaaagatcat ccctaagaga atcacgggaa
     5401 attgtgccaa gcatcagcga atggttaagg aagctattca aagggcgaga ttcatggcat
     5461 tgcttcctta tacaaaagag tgatcggatc ttgagggagg cgaagagcct ccctttttta
     5521 aggaggttct cgatgaaggt tattcttctt aaggatgtaa gtaatattgg aaaaaccggt
     5581 gaagtgaaga atgtgtcaga tggttacggt agaaacttcc ttattccgaa aggttttgcg
     5641 gttgaagcca atgcgaaaga actggccaaa ttgaagcaga tccaaaagca aaaggaagaa
     5701 aaggaagaac gcataaggaa atccagtgaa gatctactgc atgagctgca gaaacatcac
     5761 ttcataataa aggcgaagtc aggaaccagc gggaagcttt ttggagcagt tacatccggc
     5821 gacatcgcga atcacataag aaattctctt ggtgttgaaa tagacaagaa agagatagat
     5881 cttggggatc atataaaaca gaccggtgag tacagaattg atatcaaact tccaggcaac
     5941 gtaaagggta agattgcttt gaaagtagaa ggcctggagg aagagtgaat tggagtttca
     6001 aatattctcc aaggctctgt attcaacttg gattttatat aggcctgaaa ggattctctt
     6061 cgacgtcggt gaagggatct ctactatact tggaaacagc gtctatgcaa taaaggatat
     6121 ctttttgact cacggtcacg ttgatcacat atcggggctt tggggtctaa taaatactag
     6181 aaacactgct atgggagacc gaaacaagca gctaagaata aattttccgt ctggtaatcg
     6241 cgccattgaa gcgtatctgg atttcataat tggaatgaat cccagactca gatacaatat
     6301 gattataaac ccgcttaaca tcggcgatga agtctttcta aggacagcag gaagtttcag
     6361 aagacacgta accccattca gggtaaagca cacaattggt gaaatcagct atggttatca
     6421 catttacgaa gagcggagaa aacttaagga agcctattca ggactgacat cgaaggagat
     6481 cgctcgtgtt gtcaaggaaa aaggcagaga ggagattacc gatacttacc tgaagaagat
     6541 tgtcacgata agcggagata cctttgcact ttctcccgaa acagtgagtt attctgaaac
     6601 actcttgcat gaatgtactt tcctggtttc gaaggacaga agaaaccaaa accattccag
     6661 tatcgatgaa gtcatagata cagttaggag aagcagaggt ataaagagat tgatcctgta
     6721 tcatatatct gggcgatata actctcagat taagaagtgg cataatatca taaagaagga
     6781 gcttgaggat acagaaatag atgtttttct tgttcacccg gaacatgttt ttgaactgta
     6841 gtacttttct gtgtactccg ggaggaggta aagaatgggt tacataggct ggatgatttt
     6901 tggtattata ttattggtgg cggagatact aactcctact tttttctctc tgtggttttc
     6961 aataggttct ttcctggcag gccttgcagc aatgttttcg cttggactcg gttggcaagt
     7021 tctgattttc gcctcgagca gtacgttcct ggtgcttctt tccagaccga ttgccaagaa
     7081 gctatcaaaa ggagattcac caaagaaaat gtatatcgat gggatggtcg gttctctggg
     7141 acgtgtgaca gtggagatca atccggttat gggaaaaggc atggtccgga ttgatggaga
     7201 ggactggcga gctacttcgg cgaatggaga gataatacct gtagatactg tggtcaaaat
     7261 agttaggctg gaaggtactc tagtttacgt cgaaaggcaa aacaacaaca atcagtaata
     7321 gaagaaaggg ggattagaga tggttttttg gttaatactt gcagtggtaa tcttcatcat
     7381 tgcggcttca gggataaaga taattcgtcc tttcgagaaa ggactcgtag agaggttggg
     7441 aaagtataga agagatgccg agcccggtct ccagttcata atacctttca tggagagaat
     7501 ggtgaaggtt gaccttagag aaaccgtaat agatgttcca cctcaggaag ttatcacgaa
     7561 agataatgtg gtggttactg ttgacgcgat tatttattac cagataaccg atgcattcag
     7621 agttgtctac aacgtttcta atttcgagat tgctgcaata aagcttgcac agacgaacct
     7681 cagaaacgtc ataggagaga tggagcttga ccagaccttg acctcgaggg agaggattaa
     7741 cgtcaccttg agggaggtcc ttgatgaagc aaccgacaaa tggggcgtca aggttacaag
     7801 agttgaaatc aagaagatcg atccacctca ggacatcatg gatgccatgt cgaagcagat
     7861 gaaggccgag agaacaaaga gggcggttat actggaagcc gagggttaca agcagtcaga
     7921 aataacgaag gccgagggcg ataagatgtc ggcgattctg caggcagagg gtcagagtga
     7981 atcgataaag agagtggccg aggctaataa gttcaagctt gtgacagagg ccgagggcga
     8041 ggcaaacgct atcataaatg tcttcaaggc gattcatgaa ggagatccaa cgagagacgt
     8101 tattgcaata aggtatcttg atgctttgaa acaaatagcc gatggtaagg caaacaaggt
     8161 cttcctaccc tacgagagta gtgcaatgct tagttcctta ggttccatgg ccgaaatttt
     8221 taaggatgga aaagatcttt ctggcggaaa cgagaaggtt agcaagaagt gatttgattg
     8281 ttgataagtg aaacgctgag aatcttcttc agcacattgc ttattgcagg tttggccacc
     8341 gttctatcgg tggcctcaat ttttgcgttt cagcgacaaa tcaaaaaagc taattttctt
     8401 ctcctgttca tgaatgttgc cttcgcgagt ggcgttctct tcctctgcaa tatcgttttc
     8461 gtcagagaga atagacttca taatgtaatt ctctttctta tttcgtactt gtatatctat
     8521 ggatcactgg gaagacatac ttccagatat gatgacacct ttcgaatgct atttctttcc
     8581 atgggataca ctcggcagga gttcttcaag aa
//
LOCUS       DAOQYI010000060         8549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_8750_length_8549_cov_73.257123, whole genome shotgun sequence.
ACCESSION   DAOQYI010000060 DAOQYI010000000
VERSION     DAOQYI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8750_length_8549_cov_73.257123"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1317..1745)
                     /locus_tag="PKL93_09865"
     CDS             complement(1317..1745)
                     /locus_tag="PKL93_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71603.1"
                     /translation="MKGIYIFLGNLLMIVAVVIALLFGGGQIVNSVLEGKSIDIDIMD
                     FLFPITLFVTGFFLQYCSLAKRIALPVLEELLLAATLVLYIRESLAGGFSWWLLPLRD
                     YIPSSLLFTIMGYLLPGIVVFTMAALIVLMIMISGEKEEK"
     gene            complement(1801..3057)
                     /locus_tag="PKL93_09870"
     CDS             complement(1801..3057)
                     /locus_tag="PKL93_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71604.1"
                     /translation="MKKLVGILVVLLAISVAFSATITLSPTIKGTIWGHVELDKGGLD
                     TALGIKKLSVSFGAKADVTDITLDLTIVLPSDKTTVNLKSFTVENDAAALSWYAAKSL
                     AGGTDANPGLNWFKWYDDDVANVTMELVLKDLGLSIGTQEAATDKIALFGSWDIFSFA
                     VQTGLDALKWSEDFIAEATLTPLPGLDISGGVKATDLLGTATFNYVVDAKYTLNVGLL
                     TLTPRARYSDTLGQYVGLKAAYEIGVLKITGDVKYDIEANAIETWIQPVISKDGIGWA
                     GVKFGYDYDFGAATQAMTLGFLVKSNGWAAGPVSVDLYVGSGDFNTFYGNGSRKFGAE
                     IIGDVLADAKDISVYGEAALDLAMGGFNPSITAAGGYYFLDASKELVLGLTFPVLIDG
                     FNFEAGVDVFDPADTWYAGLYYNYKF"
     gene            complement(3174..4862)
                     /locus_tag="PKL93_09875"
     CDS             complement(3174..4862)
                     /locus_tag="PKL93_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNQ71605.1"
                     /translation="MKKLSILTLIVVAFASLSLGVEYSDLSENHWAYDSVIKTTAGGL
                     FIGFPDGTFRGSEFATRYQLAMTLARFMDYSDAGDAKLQEVVFSLTKKVAGLSLEISD
                     NKKNLVDLTAELRALEATVASLKQSGMTGDFVSLKTFDDTVAFLYDEIDRLSMEAVTA
                     NEGIVLISERLDSMSSVPSDVEALKNRATDLEVTVKLLGQAIANLKNDNSEQAETVAD
                     LTSRFEATENVLNSVTSDLFAMRRSMATKDDLSALESKLAGEMPDLEPYVTNEALNDK
                     LSLMYTRILKIQDSIAKMPEVKYYDTEIEEIEVALFGIDETMEVLFDQVDLNIARLDS
                     LDNQLSELKTELSAKASAGDVEAVEGRVTDLEVTTKMLSNAIIAANKRISELDYVTPE
                     QLTTKMNFLYKKLGLAEESLKAELGYLEGELALLGDEVTDNYEMLEVAFDQIDANIAV
                     LDDHADLIASLEGRTGTLEGKVGVLEGAVDSLESITNTMAIQMFKLDRNKADKSALEA
                     AETRIAALEENDVIIEGKISALEETQKQNVINTNAAIILSFVALAFGAVGLILK"
     gene            complement(4952..6148)
                     /gene="tyrS"
                     /locus_tag="PKL93_09880"
     CDS             complement(4952..6148)
                     /gene="tyrS"
                     /locus_tag="PKL93_09880"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNQ71606.1"
                     /translation="MLTPEEQLKELSRNHVSLISEEELLRKLKSKRSLRVKLGVDPSR
                     PDLHLGHAVVLRKLRHFQKFGHQVVLIIGDFTARIGDPSGRSKTRPMLSREEAKINAE
                     SYSTQAFKILDRELTEIRFNSEWLDAMSFEDVIRLSSKYTVARMMERHDFARRYEENE
                     PIGISEFLYPLAQAYDSVVVKADVEIGGDDQFFNLVVGRKIQEEHGLEAQAILTVPLI
                     EGTDGKLKMSKSYDNYIAFDDTPRDMFGKLMSIPDTLILKYFRLLTDKSEVELSEIET
                     MIKSKSVNPRDLKLELGVEITSQFYGEETALSARDEFIKIFRNKELPEEIPEIKISTE
                     SVSIVDLLVSHANISSRSEARRLIDQGGVKVNDEVLNDIHSVIEISDGDVLRIGKKRF
                     YRLVKC"
     gene            complement(6159..6410)
                     /gene="secG"
                     /locus_tag="PKL93_09885"
     CDS             complement(6159..6410)
                     /gene="secG"
                     /locus_tag="PKL93_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNQ71607.1"
                     /translation="MVVLAYVLISLHIAISVGLGIAVMMQMSKSAELGGAFGGGASHT
                     MFGRKKGLDTMGKITLGLAIGFMVNSILIAFVISRAFGA"
     gene            6507..8204
                     /locus_tag="PKL93_09890"
     CDS             6507..8204
                     /locus_tag="PKL93_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71608.1"
                     /translation="MNDKTVIVYLPLKPEMAKANNLPIKLPILVQDMGNVLDLDRIDL
                     DIIIRGLEAQCLLESDDYYESYLIYYYYEAFKRSLNSGNFDESKKFLERAGKIKKDYR
                     YHFYLGLLLREEGKIELSEIELRQAIEMNREFYIGYYELGRLFQLQEEYDEAVSSYVK
                     SVEKSGGQFPLPFVATIDCFISKGDYSAAMEVIDNVNEQFPLYSEILLRKGVILNETQ
                     KYSLAEATFSENIGLGEDWKSFYNRSFSRARQGKLFEAYDDLKTAFKISGNSEVLYEL
                     AISEKNMGMLEDSLSHCERYYNETLDEKALVLKARILDMMGDYDSAIEATEERYVELK
                     NVLLIHKYLAEGKIKRDLPFEEPLNEFYYRSLQNKIARDGFSDCRLIESGRINIERLT
                     ELLTELGIDDLTERISLLSEGLLPGKTREVTLPEVGLFTTLIFEMQFFPSQQEYLSYL
                     MSFLVSGKGKTTAIFKTLLYLERWSLSGLPFTIEAFVDEYIEEIKDLSFDFGLNIARI
                     IEYGGSDIDTFEEEQDVDPEEAVLTIIYSLEKGTISELRTPDCSLNSYITRLSGAGG"
     gene            8208..8477
                     /locus_tag="PKL93_09895"
     CDS             8208..8477
                     /locus_tag="PKL93_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNQ71609.1"
                     /translation="MNNVDELLERFFNALENDTSDLTDEDKISSILSSEFNQKERETL
                     TGVLDSLFGEVTKMVENPKEYFFSNEQEKLDEYLKEKNKKLDNPD"
BASE COUNT         2405 a         1979 c         1759 g         2406 t
ORIGIN      
        1 catatttgat ttccgaggcc atcttttcct ggtctatctt cacatctgtt ttctggatat
       61 taagatttcg ctttcttttt cttccaaaaa agcccagaag agtcaacatt ttgagagtag
      121 acctgaacct atggagcaga acaattgttt ttcagctagg aaattttgcc tgaaacagac
      181 aatctcgtct agattctgaa tgaaaggagg tgcgttgaac ataaacataa taaactttct
      241 tcagggaaca ttcgcgactc tttcggagaa atcttacaca atcgaacagg ttttcttaat
      301 ccgggcagcg caccaacgtt tagcagattg cttgctctct cttaccgtta atctcttaca
      361 aagagtagac gacacgcaat ttgaatgatc agtttgaaat gaaaattgtg aactgatctt
      421 ttggcaaagt tcaatcaaaa ctgatctgtg ttagactctt cccgaaaaac tgtatatact
      481 ggtttgttca gttcgaccct tcagtgaggt tccgtctagc agattaagaa taaataatca
      541 ctaatggaag ggctgaagag agtatctcaa ccagaaacaa ctaaggctgg atgacctgtg
      601 gtaaagttcc tgaagataca gaagatattt tcagactgac tataatgaag aattcctgcg
      661 taatctcttt ttcctccccc agtctaacac tcttgagatg tcttgctaaa gacgttcttt
      721 agtatagatt cctctgtcct ttcttccata atctcctttg ctcgccaatt ttcgaatgtc
      781 tttcagcaaa tctattttgc ggtgcttctt gtacttcaaa cgctctttaa cattcttctc
      841 cgtctgttgt ctggtcattt cacacttagc gttgtaaaaa aaggaattca agcgtttttc
      901 tgttttttgc tgaagatttg agcttcgaaa tgagaccacc tacataccta aaaagagcta
      961 tcataaggcc gactgacgct tccttctccc ttctgcagta tctctaaaac tcttttactt
     1021 caagactcta aaatccgcgc aacgtaccct caggggaaaa ctttcggttg ggttgactag
     1081 tctttcccaa caccgcactt ttcggggatt cctaagcaga gggaagcaag ctcgccaata
     1141 cttttgtatg tctgagcagt gaggaacttt ggacagctct atttatattg gctaggatag
     1201 cttctagctg ctaaagattt aaccacggaa agtttgacaa ggtttctatt gtgctagacc
     1261 ttcttacttg tttctcctaa ctgactactg cgaacagctg atctcgtgaa agcagtctat
     1321 ttctcttcct tttcaccgga gatcattatc attaatacta tcagcgcagc catagtgaac
     1381 acgactattc ccggcaacaa ataacccatg atggtaaaca gcaaagaaga aggtatgtag
     1441 tctctcaatg gaagaagcca ccagctaaac cctcccgcaa ggctctccct tatatagagc
     1501 actagagtcg cggcaagaag aagctcttct aaaaccggaa gtgctattct cttggctaaa
     1561 gagcaatact ggaggaaaaa acccgtcacg aaaagtgtaa tcggaaacag aaagtccatg
     1621 atgtcaatat caatgctctt cccttcgagc actgagttta cgatctgacc ccctccaaaa
     1681 agaagtgcaa taaccactgc aacaatcata agaagattcc cgagaaatat gtaaatgccc
     1741 ttcatatgat cacctcaaaa aaaaccggcc cgtgttgggc cggctcattt tcaacataaa
     1801 ttagaatttg taattgtaat aaagacccgc gtaccaagtg tctgctggat caaatacatc
     1861 gaccccagcc tcaaagttga aaccatcaat gagaactggg aatgtcaaac ctaatacaag
     1921 ctctttagaa gcgtcaagga aatagtagcc acctgcggct gtaatcgaag ggttgaaacc
     1981 gcccattgct agatcaagag ctgcctctcc atacacagat atatccttag catcggcaag
     2041 aacatctcca atgatctctg caccaaattt ccttgatcca tttccgtaga aggtattgaa
     2101 atcaccagat cctacataaa ggtcaacact gacaggtcca gcagcccaac cgttggactt
     2161 caccaagaaa ccgagagtca tagcctgtgt tgctgctccg aaatcataat cgtaaccaaa
     2221 cttcacgcct gcccagccta taccgtcctt ggaaatgacc ggctgtatcc aagtctcaat
     2281 ggcattcgcc tcaatatcat acttcacgtc gcctgtgatc ttgagaactc caatttcata
     2341 cgctgcttta agcccaacat actgacccag tgtgtcggag taccttgcac gaggagtcaa
     2401 tgtcaacaaa cctacgttca gcgtgtactt ggcatcaaca acatagttga atgtagctgt
     2461 accaagaaga tccgttgctt ttactccacc agaaatgtca aggcctggca aaggagtcaa
     2521 agttgcttca gcgatgaaat cttcgctcca cttgagagca tcgagtcctg tctgaacggc
     2581 aaaagagaag atatcccaag aaccgaacaa tgcaatcttg tctgttgcag cttcctgagt
     2641 accgatgctc aatccgaggt ctttcagtac caactccatc gtgacatttg ccacgtcgtc
     2701 atcgtaccat ttgaaccagt ttaaacctgg atttgcatct gtcccgccgg ctaaagattt
     2761 cgcggcgtac cagctcagtg ctgccgcatc attctccact gtgaaagact ttaggttgac
     2821 agtggttttg tccgatggga gaacaatcgt tagatcgaga gtgatgtccg taacatcggc
     2881 cttggcaccg aaactaactg ataatttttt gattccaaga gcggtatcaa gcccgccttt
     2941 gtcaagttcc acatggcccc agatcgtacc tttgattgta gggctcagag taatggtagc
     3001 cgagaaagca accgagatag ccagaagtac cactaagatt ccaacaagct tcttcatgca
     3061 cacttacccc ctttataaga gtgtttttgg tttttggtga tgcgcataac gtaaatatac
     3121 cacattttat gtaacaaaag caaactcaca tgttgctaag ttgcacagaa agatcatttc
     3181 aggattagcc cgaccgctcc aaatgcaagt gctacaaaag aaaggataat cgctgcattt
     3241 gtattgatca cattctgctt ctgagtctct tcgagagctg aaatcttacc ttcgataatt
     3301 acatcgttct cttcgagggc ggctattcta gtttcagctg cctcaagcgc agacttgtct
     3361 gccttgtttc tgtcgagctt gaacatctga attgccattg tgttagtgat gctctccagg
     3421 ctgtctactg ctccttccaa aactcctacc ttaccctcaa gagtgccagt tctaccttcg
     3481 agggaagcaa tcaaatcggc atgatcatca agaactgcga tattagcatc gatctgatca
     3541 aaggcaactt caagcatttc gtagttatcc gttacctcat ctcccaggag agcgagctcg
     3601 ccttcaagat atccaagctc tgctttcaga ctctcttctg cgagaccaag cttcttgtag
     3661 aggaaattca tctttgtcgt tagctgctcg ggtgtcacgt agtcaagctc cgaaatcctc
     3721 ttgttagctg caataattgc gttactaagc atcttagttg tgacttcgag atccgtgacc
     3781 cttccctcta ctgcttccac atcgccggct gaagcctttg ccgaaagctc tgtcttcaat
     3841 tcagaaagct ggttgtccag agaatcaagt ctcgcaatgt tcaggtcaac ctgatcaaat
     3901 agaacttcca tggtctcgtc aatcccaaat agagctactt caatctcttc aatttcagtg
     3961 tcatagtact tgacttcggg catcttggca atgctgtcct gaatcttgag gatcctcgtg
     4021 tacatgagcg aaagcttgtc atttaaggcc tcattggtta cgtaaggctc gagatctggc
     4081 atttcgcctg ctagtttcga ctcgagagcc gaaagatcgt ccttcgtagc catacttctc
     4141 ctcatagcaa aaagatctga cgtcacgctg ttaagaacgt tttcagtagc ctcaaatctg
     4201 gaagtcaaat cagcaactgt ctctgcctgc tcgctattgt cgttcttcaa attggcaata
     4261 gcttgaccga gaagcttaac tgtaacttcg agatcggttg ctctgttctt aagagcctca
     4321 acatccgaag gaacgctgct catcgaatca agtctttcgc tgattagcac gattccttca
     4381 tttgcggtga ctgcttccat ggatagtctg tcgatctcat cgtaaaggaa agcgaccgta
     4441 tcgtcaaagg ttttcaacga cacaaagtct ccagtcattc cactttgttt taacgatgcg
     4501 accgttgcct caagtgccct cagttccgct gttaagtcga ccaaattctt cttgttgtca
     4561 gagatctcca gcgaaagccc cgcgactttc ttcgtaagag agaaaaccac ttcctggagt
     4621 ttagcatccc cggcatcaga atagtccata aacctggcaa gagtcattgc tagctggtaa
     4681 cgcgtagcaa actcactacc tctgaatgtg ccatcaggga aaccaatgaa caatccaccg
     4741 gctgtcgtct taataacgct gtcgtaggcc cagtgatttt ctgacaaatc gctgtactcg
     4801 acacccaaag ataaggatgc aaaagcaaca acgatcagtg tcagtataga aagcttcttc
     4861 atggatccta ccccctttca aaatgttgtc attttctaca gcaaactacg aatttattat
     4921 acagtaaaga cacgtgcata ttcaagagta actagcactt gacaaggcga tagaaacgct
     4981 tttttccgat tctcagaaca tctccatcgg aaatttcgat aacggaatga atatcattca
     5041 agacttcatc attgactttc actccgccct gatctataag tcttctcgct tcgcttcttg
     5101 atgaaatatt agcatgagaa accagaagat cgacaattga aacactctct gtcgatattt
     5161 ttatttccgg tatctcttcg ggcagctctt tgtttctaaa gatttttatg aactcgtctc
     5221 ttgcactgag cgccgtctct tccccataga actgagatgt tatctccacc ccaagctcga
     5281 gctttaaatc ccgtgggttt accgacttgc tcttgatcat tgtctcgatt tcggaaagtt
     5341 ctacttcgct cttgtcggtc agcaatctga aatatttcag tatgagggtg tcgggaattg
     5401 acatgagctt tccgaacatg tctcttgggg tatcgtcaaa agctatgtaa ttatcgtagc
     5461 tctttgacat cttgagtttt ccgtccgtcc cttcaataag aggaacagtg agaatcgctt
     5521 gggcctcaag gccatgctcc tcctgaatct ttcttccgac gacgaggttg aagaactgat
     5581 catctccacc aatctcgaca tcggctttca caacaacaga atcgtaggcc tgagcgagag
     5641 gataaagaaa ctcggaaatt ccaattggtt cgttctcctc gtacctcctg gcgaagtcat
     5701 gtctttccat cattctggca accgtgtact ttgaggaaag ccgaataaca tcttcgaaac
     5761 tcatcgcatc cagccattcg gaattgaatc tgatttctgt aagctctctg tcgagtatct
     5821 tgaatgcctg agtgctgtaa gattcagcat tgatcttcgc ttcctctctg gaaagcattg
     5881 gtctcgtctt tgaccttccg gaagggtcac cgatcctagc cgtaaaatct ccaataatca
     5941 atacaacttg atgaccgaac ttctgaaagt gacgcagctt tctcaagacc acagcatgac
     6001 ccaagtgaag atccggtctg gacggatcta caccaagctt aaccctcaac gaccgtttac
     6061 ttttaagctt tctaagaagc tcctcctctg agatcaacga tacgtgattc ctggatagct
     6121 ccttaagttg ttcctcaggg gtcaacaact ctaccacctt atgcgccaaa tgctctcgaa
     6181 attacaaatg cgatcaaaat actgttcacc atgaatccga tagcaaggcc aagcgtgatt
     6241 ttacccatag tgtccagccc cttctttctg ccaaacatgg tatgagacgc accgcctcca
     6301 aaagccccgc ccagctccgc ggacttggac atttgcatca ttacggcaat tcctaatccc
     6361 acacttatcg caatgtgcag ggaaatcagt acatatgcaa gaacaaccat caaagaatac
     6421 ctcctgtatc ttctaacgct cttctggtat tatacaatat gtgcggattt gtagcaaacg
     6481 agaagcctga gttggcgggt ggtattttga atgacaaaac ggtaattgtt taccttcctt
     6541 tgaagccaga aatggctaag gccaataatc tcccgataaa gctcccgatc ctcgtccagg
     6601 acatggggaa tgttcttgac ctggacagaa tagaccttga cataatcata aggggtcttg
     6661 aggctcaatg ccttttggag agcgacgatt attatgagtc atacctaatt tattactact
     6721 atgaagcctt caaaagatcg ttgaattccg gcaatttcga tgaatcgaag aagttcctgg
     6781 aaagggcagg aaaaatcaaa aaggactata ggtatcattt ctatcttgga ttgttgctta
     6841 gggaggaagg aaagattgag ttgtcggaaa ttgagctgag acaagcgata gaaatgaatc
     6901 gggaatttta cattggttat tatgagcttg gcagattgtt tcagcttcaa gaggaatacg
     6961 acgaagcggt ctcttcatat gtgaagtccg ttgagaagtc tggaggacag tttcctctac
     7021 cttttgtcgc cacgattgac tgtttcattt caaagggaga ttacagtgcc gcaatggaag
     7081 ttattgacaa cgtgaatgaa cagtttcctc tttattcgga aatccttctc aggaaggggg
     7141 ttatcttgaa cgaaacccaa aagtattcct tagccgaggc gactttttcc gaaaatattg
     7201 gactcgggga agactggaag agtttctaca accgctcctt ttcaagggct agacagggga
     7261 agttgtttga ggcttatgat gatttgaaga ctgcattcaa gatcagtgga aattccgaag
     7321 tgctttacga actagccatc tcagagaaga atatgggaat gcttgaggac tctctttctc
     7381 actgtgagag atactataat gagaccctag acgaaaaggc tctggtcctg aaagcacgaa
     7441 tacttgatat gatgggtgat tatgatagcg cgatcgaggc aacagaggaa agatatgtgg
     7501 agctcaagaa tgtgcttctg attcacaaat atcttgccga aggtaagata aagagagatc
     7561 ttccctttga agaaccactg aacgagtttt actatagatc attgcagaac aaaattgcta
     7621 gagatggatt ctctgattgc agattgatcg aatccggaag gataaatatc gagaggctta
     7681 cagagcttct tactgaactg gggatcgatg atttgactga gagaatctct ctgctatctg
     7741 aagggcttct tccaggcaaa acgagagagg tcactttacc cgaagttgga ctgttcacca
     7801 cacttatttt tgagatgcag tttttccctt cacagcagga gtatctttcg tatctgatgt
     7861 ctttcctcgt ttccggcaag ggtaagacga ctgcgatttt caagactctg ttgtatcttg
     7921 aaagatggtc tctctccggt ctgcctttca caatcgaagc gttcgtggac gagtatatag
     7981 aagaaatcaa ggatctcagc tttgactttg ggctgaacat tgccaggata attgaatacg
     8041 gaggatcgga tatcgacact tttgaagagg aacaggatgt agatcctgaa gaggcggtgc
     8101 ttactatcat atattcactt gaaaagggaa caataagcga gttgaggact cccgattgct
     8161 cgctaaacag ctatattacc aggctttctg gagcaggagg ataaattatg aataacgttg
     8221 acgagttact ggaaagattt ttcaacgcac ttgagaatga tacgagcgac ctaaccgatg
     8281 aagacaaaat ctccagtatt ctgagttcgg agtttaatca gaaggagaga gaaacgctta
     8341 caggtgtcct tgattctctc ttcggcgaag tcacaaaaat ggttgaaaac ccaaaagaat
     8401 acttttttag caacgagcag gagaagctgg acgaatattt gaaagaaaag aacaaaaaac
     8461 ttgataatcc tgactgaata ggtgtataat cctttcgtag ggttacttca gcgcaaagta
     8521 ttgccggtgt tatgcaccgg tcttttttt
//
LOCUS       DAOQYI010000061         8430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_8899_length_8430_cov_79.994985, whole genome shotgun sequence.
ACCESSION   DAOQYI010000061 DAOQYI010000000
VERSION     DAOQYI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8899_length_8430_cov_79.994985"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PKL93_09900"
     CDS             <1..1326
                     /locus_tag="PKL93_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71610.1"
                     /translation="KKYPGVHYSSMWNWMKEVEIVDRLTVRVHFTEPLYQQWSFQLYQ
                     LPMVPEHIWKNKTEAEILTGANEGPIGTGCYTAEGYGQDRMIYLRNENWWAIEQLGIK
                     PTPKRIVYLTVSGNNVALGMIFKGELDISNFFLPGVPAVKSAYGIHTYFDGAPYMLSD
                     NTAVLFLNNSRKPMDDVNFRKAVAWAINADDIVTRVFENQVIKSNPLGFLPIDAWMKY
                     YDEKVVEQYGFKYDPSVSKKLLADAGYKDINGDGFVEAPDGSKIELSIIVPFGWTDWM
                     ESIKIIANNLNAVGINAKAEFPDYSRYQDELYGGNFDMAINNFNSNLSNTVWSYYYWL
                     FWDIREQQTQGNYGKYNNPKAFELMEAFDRTPVDDYEAGQKIMSELEELFLKEIPYVP
                     LWFNGMWFQASTDAWTNWPSEHGPNYYPCTWNGKWQLGGIFMLTALEAK"
     gene            1808..2800
                     /locus_tag="PKL93_09905"
     CDS             1808..2800
                     /locus_tag="PKL93_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71611.1"
                     /translation="MGAYFRRKIIIYLLTFVFAVTIDWMIPRFMPGNPITSLLSRFST
                     RSDTTEVIAGYLNEIYGLDKSPFQQYVAFWAGLFRGDLGVSIYVTGAPVTRVIARALP
                     FDLALLIPAIMLSYIAGNKFGAFAARKKKLDGIVLPIWYVLTATPYMWLGILLAWFFG
                     VALDILPIAGAYSFSMVPNWSWTFIWDFLKHWILPFSSLFLVQFGGWAIGMRNMIIYE
                     LEAEYSRYLESLGASRRLVRRYAYRNAVLPQITGLALQLGVIVAGALATEVVFSYPGI
                     GHLLKEAIMNQDYFLIQGCFLFIIVGVLLANIILDIAYILIDPRIRHTVGGETV"
     gene            2797..3645
                     /locus_tag="PKL93_09910"
     CDS             2797..3645
                     /locus_tag="PKL93_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71612.1"
                     /translation="MKIKNEFLYFALRNGRLKVGFFILVFFILLAIFGPLFAKHGPLD
                     YAGPGYRPPSKEFWLGTTTFGQDVFSQTVYGLRSTFLVGLVAGGLATILGIVIGFVAG
                     YRGGMVDELLNMLTNVVTVIPTLALLLIVAAYLPYRGILVQSIFIGLTSWPWAARAIR
                     AQTFTLRTRDFVDLARITGMKPLRIIFGEIAPNMMSYLFMTFILQFGGAILAAVTLDF
                     IGLGPTQGISLGLMMQNAVLWSAIQLGMWWWLIPPGVAITAIVGALYFMNTGLDEVFN
                     PKLREL"
     gene            3648..4598
                     /locus_tag="PKL93_09915"
     CDS             3648..4598
                     /locus_tag="PKL93_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71613.1"
                     /translation="MSLSVDGLRVYYQTLKGDIKALDGVSFDLRDGEIAGIAGESGCG
                     KSTLSNSLIMLRLPMKLVEGSASLDENPLPIGDQRKMNDFRYKRISIIPQYAMNALNP
                     TVKLKRLISDLLACHGVGYEKTLPELNRRLDLVGLDRSVLNMYPVELSGGMKQRAVMV
                     ISTLLNPSLLIADEITSALDVSTQRAVAEMIVEFRDKGFVKSVIFVTHDISILYQIAN
                     SILIMYAGKLVERASTEVIIERPVHPYTKMLISSLPEVGVHCTDRRLKGIPGKPPQLL
                     SPPRGCRFKDRCPIAFEKCEEEPPMVEIEPSHWVACWGVS"
     gene            4599..5501
                     /locus_tag="PKL93_09920"
     CDS             4599..5501
                     /locus_tag="PKL93_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71614.1"
                     /translation="MLELINLTKVFKTGMFSSGRLKAVNNVSFKIEEGEVTSLIGESG
                     SGKSTIGKMILRLLTITEGEVRLDGRDISGLKGAELRNYYRKVQGVFQDPFSSYNPIF
                     KVDRVFENLRSVFFAKMHFGDWMDKVHTVLKLVGLDPDQILGKYPHQLSGGQLQRILI
                     SRALLLDVNLLVADELISMLDASTRIDVLNLLADLKKKGLSILFITHDLSLGYYLSER
                     AVILFKGHVVEMGRTDRVFFNPQHHYTCKLLSSVPRLDKKWEDFESDHKTKIDGRKCF
                     DPIVDAEHEMVEVEEDHYVAVQRR"
     gene            5479..6873
                     /locus_tag="PKL93_09925"
     CDS             5479..6873
                     /locus_tag="PKL93_09925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HNQ71615.1"
                     /translation="MLPYKGDEDMFGEDFMFGASLSGFQFEMGRNSPESIDRGSDWYV
                     WAHDKLNIENGIVSGTFPESGPNYWTGFEEFHRLAADAGMKIIRIGLEWSRILPKPTF
                     ERSTDELNDICNNEAIEHYRRIILDIRNLGIKVMVNLNHFSLPIWLHEPIRVNRYSDF
                     TAGGWADPRAAEEFRKFAGLCARRFGDIVDMWSTENEPNVVATLGYRNRASGFPPSII
                     RPELAGVARDNLINAHLLGYEELKKETSSPVGLIYAISWIDGEEKAVDSALKAQFEFI
                     DRIRDRTDFLGLNYYSRMVVEADEESDIGYKLLPGFGQGCKPNSLSRYGRPTSDFGWE
                     IYPEGLYNILKTTMRRYDIPVFVTENGIADATDGLRPYYLIGHLKAVEKLIEEGFSVK
                     GYMHWSLTDNYEWASGLGMRFGLVSVDFTDGSLTPRPSYYLLKEIIKQGSVNGFNKML
                     KEPYDIFDESLLIE"
     gene            6888..>7234
                     /locus_tag="PKL93_09930"
     CDS             6888..>7234
                     /locus_tag="PKL93_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ71616.1"
                     /translation="MATIKDVAAEAEVSIATVSRVLNGSGYVSEDTKLRVLRVINRLN
                     YHPMPSIRKKSLYRTLGVLLPNTMGNHYGEIFMGVEDYAHKNGFNVMLAMAREMVSRE
                     QEILNEYFQRKVDG"
     assembly_gap    7235..7334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7335..7556
                     /locus_tag="PKL93_09935"
     CDS             <7335..7556
                     /locus_tag="PKL93_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ71617.1"
                     /translation="VRVPDDVSVIGFDDSVHAPYTIPALTTVSQPRIEMGSAAAQLLI
                     ERLRLTQKRVFRNIVLPTALVERDSVKAI"
     gene            7580..>8430
                     /locus_tag="PKL93_09940"
     CDS             7580..>8430
                     /locus_tag="PKL93_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HNQ71618.1"
                     /translation="MSITILGHKLSNLPWEERPKDCLEPVWRYSKNPIITRETVRGAN
                     SIFNSAVVAYKDEFRGVFRVDTKELVMELHSGRSEDGLSWNIDQERIEFNAEDPEIGR
                     FVYGYDPRVVFLEDRYYVTWCNGYHGPTIGVAYTYDFERFYQLENAFLPFNRNGVLFP
                     RKIKGKFAMLSRPSDNGHTPFGDIFYSESPDMIHWGCHRHVMSPTAGWQSTKVGAGPI
                     PIETSEGWLLIYHGVWTSCNGFVYSAGVALLDLERPWKVIKRSKHYILNPRMPYENIG
                     DVPNVTFP"
BASE COUNT         2207 a         2020 c         2166 g         1937 t
ORIGIN      
        1 aagaagtatc ccggagtcca ttacagctct atgtggaact ggatgaaaga agtggagata
       61 gtggacagac tgactgtacg tgtccacttc accgaaccac tgtatcagca atggtccttc
      121 cagctctatc agttgcctat ggttcccgag cacatatgga aaaacaagac ggaagccgaa
      181 attctgactg gagctaatga ggggcctatt ggaacgggtt gctacacagc cgaaggttac
      241 ggtcaggaca gaatgatcta cctgagaaac gaaaactggt gggctatcga gcagctggga
      301 ataaagccta cgcccaagag aatagtctat ctcaccgttt cgggaaacaa cgtggctctc
      361 ggtatgatct tcaaagggga actggatatc agtaacttct tccttccagg agtcccagcc
      421 gtcaaatccg cttacggaat ccacacatat ttcgatggcg cgccctacat gctttccgac
      481 aacacggcag ttctctttct aaacaacagc agaaaaccga tggatgatgt caacttcagg
      541 aaggccgttg cctgggcaat aaatgctgac gacatagtca cgagggtttt cgagaaccag
      601 gtaatcaagt ctaacccgct tggtttcctt cctatcgacg cctggatgaa gtactacgat
      661 gaaaaggttg ttgagcagta cggtttcaaa tatgatccct ctgtctctaa gaagctcctt
      721 gccgatgcgg gctacaagga cattaacgga gacggattcg tcgaggcgcc cgacggctcc
      781 aaaattgaac tctctatcat agttccattc ggctggaccg actggatgga gtcgataaag
      841 ataatagcca acaacctgaa cgccgtgggg atcaatgcaa aagccgagtt cccagactat
      901 tcgaggtatc aggacgaact ttacggcggc aacttcgata tggcgataaa caacttcaac
      961 agcaacctgt ctaacacagt atggagttac tactactggc tattctggga tatcagagag
     1021 cagcagactc agggcaacta cggaaaatac aacaatccca aggcctttga gttgatggaa
     1081 gccttcgaca gaacgcctgt cgatgattat gaggctggtc agaagatcat gtcggaactg
     1141 gaagagctgt tcctgaaaga gatcccctac gttcccctct ggttcaacgg aatgtggttc
     1201 caggccagta ccgatgcatg gaccaattgg ccgagcgaac atggccctaa ctactatcca
     1261 tgtacctgga acggaaaatg gcagctcggt ggtatattca tgctgacagc cctggaggcc
     1321 aagtagcaaa gtatgatctc cgcccccatg gacttccgat ccgtgggggc ctttggcaga
     1381 ccaagatcga aatatttggc tcatgtgaga ttcaacctgt caagtccggg ctcccagtgt
     1441 tgtttttcgc tgagcgagta aagagaaagt tcctgacaat gcgtctgtat tccgaaaaac
     1501 gataacgata aaggcacaaa ctggaaagat cgagataccg gactgcctat gagtataaga
     1561 ttgcacccgg atcttcacaa gcgcagtctc taaacattat cgaagcgact ttcagcatca
     1621 gtgaagaagg aaaaacctgt ccgatccacc ctctctgtca ggcgagagcc tgaagagcct
     1681 cgaaatctaa atgggtgatc ggaagggatt gagtatcgca ctttcgtcag agaatcactt
     1741 ttggttgggc aagaagatga tgaagctctc catcacgtgg gtagggtaaa ggaaggagac
     1801 tcagactgtg ggagcctatt tcagaagaaa gataataatc tacctgctga catttgtatt
     1861 tgcagttacg atagactgga tgataccgcg attcatgccc ggcaatccaa ttacgagtct
     1921 actgtccagg ttttccacca gatccgatac aaccgaggta atcgccggtt atcttaacga
     1981 gatctacggg ctggacaaat caccgtttca acagtatgtc gccttttggg cgggcctttt
     2041 cagaggtgat ctcggagtca gcatatatgt tacaggggct ccggtcacaa gggtaatagc
     2101 ccgggccctg ccctttgatc ttgccctgtt gattcccgca ataatgttga gttatatagc
     2161 cggaaacaag ttcggcgcct ttgccgccag gaaaaagaaa ctagacggca ttgtcctgcc
     2221 catctggtac gtgctgacgg caacgccgta catgtggctg ggcatacttc tggcgtggtt
     2281 ctttggggtc gcgcttgaca ttcttccgat agcgggggct tacagttttt caatggttcc
     2341 caactggagc tggaccttca tctgggattt ccttaagcac tggatacttc ccttcagctc
     2401 tctctttctc gtgcagtttg gaggttgggc tataggaatg cgcaacatga tcatctacga
     2461 acttgaggcc gaatattcca gataccttga atctctggga gcctcgagaa gactggtcag
     2521 acgttatgcc tacagaaatg cggttcttcc tcagatcaca ggactggctc tccagcttgg
     2581 tgtcatagtc gccggggccc ttgctacaga agtcgtcttc tcataccccg gtatcggaca
     2641 tcttctgaag gaagcgataa tgaatcaaga ttactttctc atacagggct gttttctgtt
     2701 cataatcgtc ggcgtgttgt tggctaacat cattctggat atcgcgtata tactgatcga
     2761 tccgagaatc aggcacacgg tagggggaga gactgtatga agattaagaa tgagtttcta
     2821 tacttcgccc tgaggaacgg gcgtttgaag gtcggctttt tcatactcgt gttctttatc
     2881 ttgctggcga tcttcggacc tctcttcgcg aagcacggac cgctcgatta tgcgggtccg
     2941 ggctaccgtc cgccttcgaa agagttctgg ttagggacca caaccttcgg gcaggatgtt
     3001 ttctcgcaga cggtttacgg gctgaggtca accttcctgg tcggattggt tgctggaggt
     3061 ctcgcaacca ttctgggaat cgtaatcggc ttcgtagcag gttatcgcgg aggcatggtc
     3121 gacgagctgc tcaatatgtt gaccaatgtg gtcaccgtga taccgacgct ggccctcctt
     3181 ctaattgttg cggcttatct gccatacagg ggaatactcg tccagagcat atttataggg
     3241 ctcacttctt ggccgtgggc cgcgagagcg ataagggccc agacgtttac tttgagaacg
     3301 agggactttg tggacctcgc aagaatcact ggcatgaaac ccttgagaat aatctttgga
     3361 gagatcgctc ccaacatgat gtcctatctt ttcatgacct tcatactcca gttcggagga
     3421 gccattctcg cggccgttac gcttgatttc atcgggctag gtccgactca ggggatttct
     3481 ctaggactaa tgatgcagaa cgcggttttg tggagcgcaa tccagctggg aatgtggtgg
     3541 tggctgatac ctcccggagt agccattacg gctatcgtcg gcgcgctcta ctttatgaat
     3601 actggactgg atgaagtctt caatcctaaa ttgagggagc tgtgacgatg tctctaagcg
     3661 tggatggcct tagagtctat taccagactc tcaagggaga tattaaggca ctggacggcg
     3721 tgagcttcga tcttcgtgat ggtgagatcg caggaattgc cggagaatcg ggctgcggaa
     3781 aatctactct cagcaactcc ctcataatgc ttcgccttcc gatgaaactc gtggagggca
     3841 gtgcctctct tgacgaaaat ccactcccaa taggcgatca aaggaagatg aacgacttca
     3901 gatacaagag aatctcgata atccctcagt atgccatgaa cgccttgaat cccaccgtga
     3961 agctcaagag actcatctca gatcttctcg cctgtcacgg cgtgggttac gaaaaaactc
     4021 tccccgaact caacaggaga ctggatctcg ttggtctgga tagatcggtc ctgaacatgt
     4081 atcccgtaga actgtcggga ggaatgaagc agagggccgt aatggttatc tcgactcttc
     4141 tgaatccctc tctgctgata gccgacgaaa tcacatcggc cctggatgtc tcgactcagc
     4201 gagcagtggc cgagatgatc gtggagttca gggataaggg cttcgtcaag agcgtcattt
     4261 ttgttacaca cgacatctcc atcctctacc agatagccaa ttctattcta ataatgtacg
     4321 ccggtaagct cgtagaacgg gcctccacgg aagtgataat tgaacggccc gtccatccct
     4381 atacgaagat gcttatctcg tctctgccgg aggtcggcgt tcactgcaca gatagaagac
     4441 tgaaagggat ccctggaaaa ccccctcaac tcctctcacc tccgcggggt tgcagattca
     4501 aggacagatg cccgatcgcc ttcgaaaaat gcgaagagga accacccatg gtcgaaattg
     4561 agccttcaca ctgggtggcc tgctggggag tgagctagat gcttgagctc attaatctca
     4621 cgaaggtctt caaaacggga atgttttctt caggcaggct aaaggccgtg aacaatgtct
     4681 cattcaagat cgaagaggga gaggtcacct cgctcatagg cgaaagcggg agcggtaaga
     4741 gcaccatagg aaagatgatc ttgagattgc ttacaatcac cgaaggggaa gtaagacttg
     4801 acggacgtga tataagcggg cttaagggcg cagaactaag aaactactac agaaaggtcc
     4861 agggcgtctt tcaggatccc ttcagctcct ataatcctat cttcaaagtt gacagagtct
     4921 tcgagaatct tagatcggtc ttctttgcga agatgcactt cggcgactgg atggataagg
     4981 tccacactgt cctgaaactg gttggcctcg atccggatca gatcctgggc aagtatcctc
     5041 atcagctgag cggcgggcag ctccagagaa tcctaatatc cagggccctg ctgctggatg
     5101 tgaaccttct cgtcgccgac gaactgatca gcatgcttga cgcctctaca agaatagacg
     5161 ttttgaacct gctcgcagac ctcaaaaaga aggggctttc aatcctcttc ataactcatg
     5221 acctgtctct cggctactat ttaagcgaaa gagctgtgat actgttcaag ggtcatgttg
     5281 tagaaatggg aaggactgac agggttttct tcaaccccca gcaccattac acatgcaagc
     5341 ttctctcttc agtaccgagg ctcgacaaga agtgggagga ttttgaaagc gatcacaaaa
     5401 cgaaaataga cggacggaag tgtttcgatc ccatcgtgga cgcggaacat gaaatggtgg
     5461 aagttgaaga ggatcactat gttgccgtac aaaggagatg aagacatgtt tggcgaagat
     5521 ttcatgttcg gtgcctctct ttcgggattt cagtttgaaa tgggtaggaa ctctcctgag
     5581 agcatcgacc gcggctcgga ctggtacgtt tgggctcatg ataaattgaa catcgaaaac
     5641 gggatagtca gcggaacgtt tcccgaaagc ggaccgaatt actggacagg tttcgaggag
     5701 tttcacagac ttgcggccga cgcgggaatg aagataatac gaataggtct ggagtggtct
     5761 agaatcctcc ccaagccgac tttcgagaga tccacagatg aactgaacga catatgcaac
     5821 aacgaagcga ttgaacacta cagaaggatc atcctagaca taaggaattt gggaatcaag
     5881 gtgatggtaa atctcaacca tttctcactc cccatttggc tccacgaacc aattagggtc
     5941 aacagatact ccgatttcac cgccgggggc tgggccgatc cccgtgcagc ggaggaattc
     6001 cgcaaatttg ccggtctctg cgcgaggaga tttggagaca tcgtcgacat gtggtcgacc
     6061 gagaacgaac cgaacgtcgt ggctacgctt ggctacagaa acagggcctc cgggttccct
     6121 ccctcgataa tcaggcccga actggcgggg gtggctcgcg acaatctgat caatgcccat
     6181 ctacttggat acgaggaact aaagaaagaa acaagctctc cagtgggact gatctacgct
     6241 attagctgga tagacgggga ggaaaaggcg gtcgattctg cgctgaaagc gcaatttgaa
     6301 ttcatcgacc gtataagaga tagaacggac tttcttggct tgaattacta ctcgcggatg
     6361 gtagtcgaag ccgacgaaga gagcgacata ggttacaaac tccttccggg attcggacag
     6421 ggctgcaagc ccaactcgct ctccagatat ggcagaccga ctagtgattt cggctgggag
     6481 atctaccccg aagggttata caatatattg aagaccacta tgagaaggta cgacatcccg
     6541 gtcttcgtaa ccgagaatgg aatcgccgac gccacggacg gactgaggcc atactacctc
     6601 ataggtcacc ttaaagcagt ggaaaagctt attgaagagg gcttcagcgt gaagggatac
     6661 atgcactggt ctctcacaga caactacgaa tgggcctccg gcctcgggat gcgtttcgga
     6721 ctggtcagtg tggacttcac agatggaagc cttactccga gaccctccta ctaccttttg
     6781 aaagaaatca taaaacaggg ttcggttaat ggtttcaaca agatgctgaa agagccgtac
     6841 gacatatttg acgaaagcct gctcatcgaa tagggggaaa agactggatg gcaacgataa
     6901 aagatgtggc cgccgaagcc gaggtttcga tagcgaccgt ctctcgggta ttgaacggaa
     6961 gcggctatgt ttccgaagat acgaagctgc gcgtgttgag ggtaatcaac agactcaact
     7021 accatcccat gccttctatt cgaaagaaga gcctctacag aaccctgggt gtccttctgc
     7081 ccaacacaat gggaaaccat tacggcgaaa tcttcatggg agtggaggat tatgctcaca
     7141 agaacggctt caacgtgatg ctcgccatgg ccagagaaat ggtcagtcgt gaacaggaga
     7201 ttctgaacga atacttccag cgaaaggtag atggnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnngttcgt gtcccagacg acgtatccgt gatcgggttc gacgattccg
     7381 ttcacgcgcc ctatactatc ccagcattaa ccaccgtctc gcaaccgaga atcgaaatgg
     7441 gaagcgccgc agcccagctg ctaatagaaa gattgaggct tacccagaag agagtattca
     7501 ggaatatagt tctgcccacg gccctcgtcg aaagggactc ggttaaagcc atataggaga
     7561 gaggaaggag aggatctctt tgtcgatcac aatactcggt cacaaactca gtaatctccc
     7621 ctgggaagag agacccaaag attgtctaga accggtctgg agatactcga aaaaccccat
     7681 catcacacgg gaaacggtga gaggcgcaaa cagcatcttc aacagcgccg tcgtagccta
     7741 caaagatgag ttcaggggag tattccgtgt agatacgaaa gagcttgtca tggagcttca
     7801 ctcgggaaga agcgaagacg gattgtcgtg gaacattgat caggagagaa tcgaatttaa
     7861 tgccgaagac ccggaaatcg gccgattcgt ttacggatac gatcccagag tcgtcttcct
     7921 ggaagacaga tactacgtaa cctggtgcaa cggttatcac gggcctacca taggcgttgc
     7981 ctacacttac gatttcgaga gattttatca gcttgagaat gcattccttc ccttcaacag
     8041 aaacggggtc ctcttcccga ggaagatcaa aggaaagttt gcaatgttga gccggccgag
     8101 cgacaacggc cacactccct ttggagacat cttctacagc gagagccccg acatgattca
     8161 ttggggctgc cacaggcatg taatgtcacc caccgccggc tggcaatcga ccaaggttgg
     8221 ggccggacct attccgatag aaacctcgga aggttggctg ctaatctatc acggagtatg
     8281 gacatcctgc aacggcttcg tgtacagtgc cggagtggcc ctcctcgatc tggaaagacc
     8341 atggaaagta atcaaaaggt cgaaacacta catactcaac ccgagaatgc cttacgaaaa
     8401 cattggcgac gtccccaacg tcaccttccc
//
LOCUS       DAOQYI010000062         8200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_9221_length_8200_cov_62.912584, whole genome shotgun sequence.
ACCESSION   DAOQYI010000062 DAOQYI010000000
VERSION     DAOQYI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9221_length_8200_cov_62.912584"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..1485)
                     /locus_tag="PKL93_09945"
     CDS             complement(229..1485)
                     /locus_tag="PKL93_09945"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNQ71619.1"
                     /translation="MRFLEQYLEKEVRPALGCTEPGAIAFCVALAASKLEGKTEKVHV
                     TTSVNVYKNGMYVGIPGTNEKGNEFAAALGAICGDADLQLEALRPCRVESVGIAREMI
                     DKGLVSVICDQDLSGVFIEATVSNHNHSVLCRITGSHTNVVSLEIDGEKVRSSERFVP
                     SDALEFTPEEIFESIREITELEIEKIFEGIEMNLEIARFGLKKMLPSKETFSYHENVS
                     LGYKIRNYCFAASAARMSGVPLPVMSSSGSGNQGIVATLPVALTGEYFQKSRKDIAKA
                     VALSHLFSGYIKNKLGRVAPICGCVTAAGTGAAAGITYLLGGARETIEQAMLTILSST
                     TGIMCDGAKESCSLKAGLGGQEAYSCALLAMDNLGVNSEQGFISNTLDRSIENIQVLL
                     SKGMSKIDSAIVEVLENRKRKTKSPE"
     gene            complement(1957..3081)
                     /locus_tag="PKL93_09950"
     CDS             complement(1957..3081)
                     /locus_tag="PKL93_09950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNQ71620.1"
                     /translation="MSRKFAMEFVLAIFLMAHGLVFSFPVVCGDLGQFTFTDAIFYLR
                     KLIFGTISRPYEENYQAELPDSACAITITALGDLMYSRGLVIAGDPYSSVEKSLFQSE
                     IVFANLEFPIDTRFPESAFPKFNGSSEYFEKVVTPLGINVMNIANNHSLDRGIEGLRS
                     TRTFLNDRGIHCVGFGEESRFSIISFDEVRIAVSGFTFGTNGATVNSESSLNVANLNT
                     FEDAETPLSRIVPIVESMSEEASVIVLSLHWGLEFENKPTEMQVEVARRLCDEGVDLI
                     IGHHPHVLQPLEVYLNARGSTSLIIYSLGNWISSMKAKDCRITASLKIELSKDGSVCG
                     IKVFPYFFNLSKMQLVPLSNPALIPESFAYYLDSSPYCSH"
     gene            3851..6199
                     /locus_tag="PKL93_09955"
     CDS             3851..6199
                     /locus_tag="PKL93_09955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ71621.1"
                     /translation="MESDSRNLFSSVRRIISVLVGNEKSLEKIKKALSTVGDSLSVNS
                     LTLCTVERAHHGSSFLKMVCDWRKESDSVMCVCGSRTGDSRKSELEKLMRKGRSNTFS
                     EAGKGILLIKSPPGLPECVLVLEIELHERNLSVEEKDFVKCAIQLLALMTGEEFSEET
                     GNNEKSLLQERLLRSDELLKKFEGNVLDILAVVDGRLVFTYVSPSVVNTLEFVAEELV
                     GKNALDLIDFRDRERVIEESKVLLREKKHGCFRYRARTKSGGILWMETAVSPLFNGKE
                     VEGWTMSSRDVTGEMKLQAELKARDSMMVFMNDLYVLILSGDNLHEAASKIVNRAYEE
                     KGMVGCKITFSEDEYLDLEVSRGDTEAFPLNLMKDSKAQETLRENRIIVQNTIIEGKE
                     LRYICIALESNNEIAGSFETLTERVTGLTEESMSLYRLVGTTVSLSLTKIRNLVATKR
                     NHITSQSLIKATEAITKDLNMRTVIESIVGGLRQVIPFYSAAVELVEGDHLIIVGGSW
                     PGKDSFVGTRFTMEEGSPGFDVVKNKKIILAEDAQRAYQQFNKPDFIFIKSWMGVPLV
                     IGDETIGMIAVDSEEGGKFKKRHLDALKAFADIAAIGINNARLHEEVRDLSVRDYLTG
                     AYNRRGLFELGECEIEKAKRYDTKMGLIMFDIDNFKNMNDMLGHLAGDFVLKRVANIC
                     TKILRAADIFARYGGDEFIILLPMTDITNTEEAATRICRSLEDSRIIYKRIPIKVRAS
                     LGIASLEGHGDELEDMIRRADRNLYVSKRSGGNKVTGGRNYG"
     gene            6192..6392
                     /locus_tag="PKL93_09960"
     CDS             6192..6392
                     /locus_tag="PKL93_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71622.1"
                     /translation="MAKREWQIRSERLGLEAQLRERNKELKLLYDFSRLVDKAEKSID
                     DILMGLLRILSDAFQNLRARVP"
     gene            6607..7035
                     /locus_tag="PKL93_09965"
     CDS             6607..7035
                     /locus_tag="PKL93_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ71623.1"
                     /translation="MLIENKEQYRANLSSIGDAVLSMDAFGIVTFVNEVACSLIGLSE
                     SSTIGKRVSEVMEIFSEDSDEPAIIPVEHVLETGKKVGLANHTALRSKNGKIYSIADS
                     AAPISREDGETTGVILVFKDLTEERKKKRKSYKVRPDSKS"
     gene            7168..>8200
                     /locus_tag="PKL93_09970"
     CDS             7168..>8200
                     /locus_tag="PKL93_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ71624.1"
                     /translation="MFFTGVEKSSLFEAMRKVYNTGTPEKVEAEKYVYGDQEGWWTNY
                     VNKLPSGEVVSLCYDLSESLRIQEFRKKTNDGLLALSKMAAGSGFSLDEFKKSAERIV
                     GSCLCVHSVGIYMLKRIGEMDLIISSDLVGGEAREDKSRAMDHDLPSEYLQLIQAKRF
                     FSISEVNEEEKSNFIASLQDVGEKAILDIPLRIRGVIVGPLFCDMKIARQWTSNEKNF
                     AVSVGDVLTLALEEDELVNGEHRYKSFFKMNGATMLLIDSTTGSIVDSNEAACRFYQY
                     DLDTLKGMSMKDLAANNESLIEIRKLAAGRSPSVVSRQKKSSGKIVDVEIFASPFSME
                     DMDLINLIVV"
BASE COUNT         2436 a         1610 c         1917 g         2237 t
ORIGIN      
        1 cacctcaaat actggaaccc actggttgca tccagtaagg ccctttctct ttagtgatca
       61 aagtatagaa cgcgaagaac agaacgtttc atgtataggg taatccttat tttatgtgtg
      121 attatgtgga gatttctctt attgacattt agaacgtcaa gagttccttg atcttcggcc
      181 ttgcataagt agagttttct tcaaggatcg acatatagtc catccctttc actcagggga
      241 tttcgttttc ctttttctgt tttcaagtac ctcaactatt gcggagtcga tctttgacat
      301 gccttttgaa agaaggacct ggatattttc tattgacctg tctaaagtgt tggagataaa
      361 tccctgctct gagttgactc caagattgtc cattgccagt aatgcacagc tatatgcttc
      421 ctggcctcca aggcccgcct tcaatgaaca gctctctttt gcaccatcgc acatgatgcc
      481 tgtcgtgctc gatagtatag tcagcattgc ctgttcgatt gtctcccttg ctcctccaag
      541 aagataggta attcctgcag cggctcccgt tccggcggct gtaacgcatc cacagatcgg
      601 ggcaactctg ccgagcttgt tctttatgta accagagaaa aggtggctca aagctactgc
      661 ttttgcaatg tcttttcggc tcttttggaa gtactcgccg gttagagcca ctggaagtgt
      721 ggcgacaatt ccctggttac cgcttccgct actgctcatc acaggaaggg gaacaccaga
      781 catcctggcg gccgaagccg cgaaacaata gttacgaatc ttgtatccga ggcttacgtt
      841 ttcgtggtag ctgaaggtct cttttgaagg caacattttc ttaaggccaa atctggcaat
      901 ctcgagattc atttctattc cctcgaagat cttctcgatt tccagttcag tgatttctcg
      961 aattgactca aatatctctt caggagtaaa ttctaatgca tcggagggga cgaatctctc
     1021 acttgaacgg accttttctc cgtctatctc taatgaaact acattcgtgt gactgccagt
     1081 tatcctgcaa agtacagaat gattatgatt agacaccgtg gcttctatga atactccaga
     1141 tagatcttga tcacagatca cactgaccag acccttgtct atcatctctc ttgcaatacc
     1201 gaccgattca actcggcaag gcctaagtgc ctcgagctgg agatctgcat ccccgcatat
     1261 ggcgcccaaa gcggcagcaa actcatttcc cttttcattc gtacctggaa ttcccacgta
     1321 cattccgttc ttgtatacgt tgacgctggt agttacatgc actttttctg tttttccctc
     1381 aagtttgctt gctgcaagag caacacaaaa tgcaatcgct ccaggttccg tacacccaag
     1441 tgcaggtcta acttcttttt caaggtattg ttcaagaaag cgcatctttc acctccaatg
     1501 tccgataact tagtttagca ttggaaatgt tttatgcaat taatagcagg cctcaaaggt
     1561 tattgttttg aagtaagtat gagctcttaa ctccctcttc cacttggaac tgaaagtata
     1621 ctcaacattg tggagctgga aaatcatctc ttggaaggct ttcttttctc gttttaaaga
     1681 gaaagacgaa ctttccagag ttctttatct agaatcggtc atgtctcata ctgaattaaa
     1741 ctcttccaag agggctaaag cagtgttcct gatagtcact tcaatctcct ttgatgttac
     1801 ataaaaaatc tggaaggaca aatttgacaa ttattggatt gtgttagaat ccctttattc
     1861 gctgaaatac tgactgagaa aggcaatgac ggaagattgt tatcagaaaa gaccataaat
     1921 cagtcaggtg aggataacta gtcggtactg gaatccttag tgtgaacaat atgggctact
     1981 atccaaatag tatgcaaatg attcggggat aagggcagga tttgacagag gcacaagctg
     2041 catcttgctt agattaaaga aataagggaa aaccttgatt ccacaaacac tcccgtcttt
     2101 ggaaagctcg atttttagcg atgctgtgat tctgcaatcc ttagctttca tagaagaaat
     2161 ccagttgccc agagaataga tgattagcga agttgatcct ctcgcattta agtacacctc
     2221 cagtggttga aggacatgag gatgatgacc gataatcaag tcaactcctt catcgcagag
     2281 ccttcttgcg acttccacct gcatttcagt aggtttgttc tcaaactcaa gtccccagtg
     2341 aagactaagc acgataacgg atgcttcttc cgacatagat tcaacgatag gaacgatcct
     2401 tgacagcgga gtctctgcat cctcaaatgt gtttaggttg gcaacattca gactggattc
     2461 actattcacg gtggctccgt ttgtgccgaa cgtgaaacct gagacagcga ttctaacttc
     2521 atcgaatgaa attattgaga atcgactttc ttctccaaat ccaacacaat ggattccacg
     2581 gtcattaaga aaagtacggg tcgatctcag tccctcgata cccctgtcaa gagagtgatt
     2641 gtttgcgata ttcataacat taattcccaa aggcgtcact actttctcaa agtattcgga
     2701 agaaccgttg aattttggga atgccgattc ggggaaccga gtgtctattg ggaattcgag
     2761 attggcgaac acaatttcac tttgaaaaag actcttctcc acacttgagt aaggatcgcc
     2821 cgcgattaca agccctctac tgtacattag atcgccaagt gcggttatag ttattgcaca
     2881 tgccgaatca gggagctctg cctgatagtt ttcttcgtaa ggtctcgaaa tcgtaccgaa
     2941 gatcagcttc cgcaggtaga aaatggcatc tgtaaaagtg aattggccaa ggtcgccaca
     3001 aacaacgggg aaggaaaaaa caaggccatg tgccattagg aaaatagcca gaacaaactc
     3061 catagcaaac tttcgagaca tctgtaccac ttcctgtaag tgattagaaa tttatagtct
     3121 atatgagtca agtccatccc acacatgatt atggttttcg agaaccatct cgccggaaac
     3181 tcagctaccg gataatcagc taccggataa atagatatca gttctcgact aaactttaac
     3241 actatacaca cacagatgat aagcatttga tggtcaatat ccttcgtcgg tgagaaagag
     3301 attctggtcg gagatgtttc tcggcaaaat taatagttca gttggcacca aattggaaga
     3361 tgagacttga ggattatgta ctcagtgcct tggtcgaaga tgggtataaa aaattctttt
     3421 aagcttctca ttgctaagtt gaatcattgg tctgaagtat cagcggcatt agatgagagc
     3481 cacaaccgat gacaatggag agttagcctt cggtatctca gagaacagat aggtcaatgg
     3541 aatgtgcgtg actctaagta ataaatgtca ttaacaggaa ggtgtcactt ggtcatctgc
     3601 agggtgacac aaacagtttt gatgagagaa cacgttaatg tccccgaagg atgcagagga
     3661 gcaatcagat cgcaccaaaa gaatgactct aagatatctt agaggtcaaa cagaaaatag
     3721 atcacagcta aactgcggct tcttccatgt ttacaaggac attatctctg cgtttgattt
     3781 ttggcacgaa atggcaatgc tataatcaac tgagaacatt acagcgcctc atcattcctc
     3841 ggagggtcga atggagtccg acagtagaaa cctgttcagt tcagtgagaa gaataatctc
     3901 ggttctcgtt ggcaatgaga agagcttgga aaagattaaa aaggctcttt caactgttgg
     3961 tgattctctt tctgtcaata gtcttactct ttgcacagtt gaaagagctc atcatggttc
     4021 ttctttcctc aagatggttt gcgactggag aaaagagtct gactcagtaa tgtgcgtctg
     4081 cggttcgaga actggagata gtagaaaatc ggaacttgag aagctaatga gaaaaggcag
     4141 atcaaataca ttttcggaag ctggaaaagg gatcttgtta atcaaaagcc cccctggatt
     4201 gccggagtgt gttctggtct tagagatcga attgcatgag agaaatctgt ctgtggagga
     4261 aaaggacttt gtgaaatgtg cgattcaatt gttggcgctc atgactggag aagagttcag
     4321 cgaagagact ggaaataacg aaaagtcact tcttcaggaa agactactaa gaagcgatga
     4381 actgctgaag aagttcgaag gcaacgtctt ggacattctt gccgtagttg atggaagact
     4441 ggttttcacc tatgtttcgc cctcggtagt aaacacattg gagtttgtgg ctgaagagct
     4501 tgtagggaag aatgctctag atcttattga ctttagggat cgtgaaagag ttattgagga
     4561 gtccaaggtt cttctgagag aaaagaaaca cggttgcttc agatatagag caagaactaa
     4621 gagcggcgga atattgtgga tggagacagc tgtaagtcct ttgtttaatg ggaaagaagt
     4681 cgagggttgg acgatgtcgt cacgggatgt caccggtgag atgaagctgc aggccgaatt
     4741 gaaagccaga gatagtatga tggtcttcat gaacgatctt tacgttctga ttctgagcgg
     4801 tgataatctt catgaagcgg cttccaagat tgtgaatcgc gcatacgaag agaagggaat
     4861 ggtcggttgc aagatcacat ttagtgagga tgaatatctt gatcttgagg tttcgagagg
     4921 cgacaccgag gcatttccgc tgaatctcat gaaggactca aaggctcagg aaaccctcag
     4981 agaaaatcga ataatcgttc aaaatacaat catagaagga aaggaactga gatacatatg
     5041 cattgcactt gaatcgaata atgagattgc tggttccttt gaaaccctta cagaacgtgt
     5101 gacaggtctt accgaagaaa gtatgagtct ctataggttg gttgggacta cggtttctct
     5161 ttctctcacg aaaatcagaa atcttgttgc tacgaagaga aaccacatca cgtcgcagtc
     5221 attgattaag gcgaccgaag cgattactaa ggatctgaac atgagaacgg taatcgaatc
     5281 tattgttggg ggtctaagac aagtaatacc attctacagt gccgcagttg agttagttga
     5341 aggggatcat ctaatcattg tcggcggtag ctggcctgga aaggattctt ttgtcgggac
     5401 cagattcaca atggaagaag ggagtccggg cttcgatgta gtaaaaaaca aaaaaatcat
     5461 tctagcagaa gatgctcaga gagcgtacca gcaattcaac aaaccagact tcatattcat
     5521 caaatcctgg atgggcgttc ctctggttat tggagatgaa acgattggaa tgatcgccgt
     5581 tgacagcgaa gaaggcggaa agtttaagaa gcggcatctt gatgccctca aggcatttgc
     5641 agatattgct gcgattggca taaacaatgc ccgtcttcac gaggaggtga gagatctctc
     5701 ggtgagagac tatctcactg gtgcttacaa caggcgaggt ctgtttgagc taggagaatg
     5761 tgaaatcgag aaggcaaaac gatatgacac aaaaatggga ttgataatgt tcgacataga
     5821 taattttaag aatatgaatg acatgcttgg acatctagcc ggagactttg tcttaaaaag
     5881 ggttgcaaac atatgtacca agattctacg agccgcagat atttttgcga gatatggtgg
     5941 cgatgaattc ataattctgc tcccgatgac tgatatcacg aacaccgaag aagctgccac
     6001 aaggatttgt cgttctcttg aagattcaag aataatatat aagagaatcc caataaaggt
     6061 tcgtgccagc ctgggaattg ccagtcttga aggacatgga gatgaacttg aagacatgat
     6121 caggagggca gacagaaatc tgtatgtctc caaaagatcc gggggcaaca aagttaccgg
     6181 gggacgaaac tatggctaag agagaatggc agataagaag tgaacgttta ggccttgaag
     6241 cgcagctaag ggaaagaaat aaagaactta aacttctata tgatttctca agattagttg
     6301 acaaagcaga gaaatctatt gacgacattc tgatgggtct cctgagaatc ctttccgatg
     6361 cttttcagaa cctgagagcg cgtgtgccgt gatcgtctac agtgccagag tgtatgaatc
     6421 gagaagagtc ttcaagaagt gatcgcttta tacgtaacca atgatagtaa gaggcaatga
     6481 ggtcgggagc gtaacggtct attattattg agacgggaaa atcgccttcc tctatgagta
     6541 gtctcaaatg gttctctcgg taacagagag actcgggaaa ataattcaat catcgaggtg
     6601 tcaaggatgc tcattgaaaa caaagagcag taccgcgcta acctctcaag tattggtgat
     6661 gcagttcttt cgatggatgc ttttggaata gtcacttttg ttaacgaagt tgcctgcagt
     6721 cttattggtc ttagcgaaag cagcaccatc ggcaagagag tttctgaagt tatggagatc
     6781 tttagtgaag attcagatga acctgcaatt attccagtag agcatgtgct agaaaccggc
     6841 aaaaaggttg gactggcaaa tcatactgcg ctcagatcaa agaacggaaa aatctactca
     6901 attgccgatt ctgcagctcc gatatctaga gaagacggtg aaacaactgg tgttattctt
     6961 gtcttcaaag atctgaccga agagagaaaa aagaagagaa aatcatacaa agtgaggccc
     7021 gattccaaga gttgatagaa aataagaatg gaggcctgtt aattttaaac agttaagatg
     7081 gagaacgatt tattctgaag gaattcaaag cgcctcgcga aaatcttaat agaactggac
     7141 tcgtaacctt tggctaggag ttaattgatg ttttttactg gtgtagaaaa gagtagtctc
     7201 ttcgaagcaa tgagaaaggt ttataatacc gggacccctg agaaagtaga ggccgagaaa
     7261 tatgtctacg gagatcaaga aggctggtgg acaaattatg ttaataaact tccttctggt
     7321 gaggttgtaa gcctttgcta cgatttgtct gaatcactta gaattcaaga attcagaaag
     7381 aaaacgaacg atggcctgct tgcactgtca aaaatggctg ctgggagtgg tttctcgctt
     7441 gatgagttta agaagagcgc cgaaagaata gtaggctctt gtctttgtgt tcatagtgtc
     7501 gggatatata tgctaaagag aattggtgag atggatctga ttattagctc tgacctggtt
     7561 ggtggagagg caagagaaga taagtcgaga gctatggatc acgacctccc ttccgaatat
     7621 cttcagttga ttcaagcaaa gaggttcttc agtatttccg aagtgaacga agaagaaaaa
     7681 agcaacttca ttgctagtct gcaagacgtt ggagaaaaag cgattctaga tatccccctg
     7741 aggatcagag gagtcatcgt gggacctctc ttttgcgata tgaagattgc aaggcaatgg
     7801 acttcaaatg aaaagaactt cgccgtgtcc gtaggtgacg ttctgacgct tgctcttgaa
     7861 gaagatgagc ttgttaatgg tgagcatcgc tacaagagct tcttcaaaat gaacggtgca
     7921 acaatgcttc tcatagattc gacaacgggt tcaatcgtag attctaacga agctgcatgc
     7981 agattttacc aatatgatct tgacacgcta aaaggaatgt caatgaagga tttggctgcg
     8041 aataacgaat ccctaattga gatacgtaag ctcgctgctg gaagatctcc tagtgtagtc
     8101 tccaggcaga aaaagtcctc tggaaaaata gtcgacgtcg agatttttgc ttctcctttt
     8161 agcatggaag atatggatct gattaacctg atagttgtcg
//
LOCUS       DAOQYI010000063         8174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_9257_length_8174_cov_82.791230, whole genome shotgun sequence.
ACCESSION   DAOQYI010000063 DAOQYI010000000
VERSION     DAOQYI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9257_length_8174_cov_82.791230"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1058)
                     /locus_tag="PKL93_09975"
     CDS             complement(72..1058)
                     /locus_tag="PKL93_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ71625.1"
                     /translation="MKILSTEGLKKFFPIKAGVFFQVVGHVRAMQSVTLEISRGETIG
                     VVGESGCGKSTLGRTIVKIYEPTGGKILYHDENGEEHDITKGLGKNVRSKFRRDVQMI
                     FQNPFDSLDPRMTVRDIIREPIEAHNLLSKKEAEDYVGELLMKVGLYPEYAQRYPHEF
                     SGGQRQRIAIARSISVRPRLIICDEPTSALDVSVQSQIINLLQELREESEMSYIFISH
                     NLDVVHHMSDRIMVMYLGNVVETAEARELFNNPAHPYTKALMASIPNWDPEDRKLQNI
                     KLEGEPPSPINPPSGCPFHPRCPYKMDICSEEMPGNFEVAEGHKVACWLHKT"
     gene            complement(1055..2032)
                     /locus_tag="PKL93_09980"
     CDS             complement(1055..2032)
                     /locus_tag="PKL93_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71626.1"
                     /translation="MSAILEVKNLKTYFRTPDGLVKAVDDISFYLEKGEILALVGESG
                     CGKSVTVQTILGLIKVPPAKVEGEILYKGKNLVGLSNKAYRDIRGKEISMIFQEPMSA
                     FDPLYTIGFQLTEVARAHMNWDQTRTYDEIISILRRVNIPEPEKRYREYPHEMSGGML
                     QRIMIAMALITNPEIIIADEPTTALDVTIQAQVLNIMRELQGEFGSSIIFITHDLGVV
                     AELADRVHVMYAGKIVEKAGVKELYSSPSHPYTKGLLSSRVTRSFKGKKLPYIEGYVP
                     RAYEFPEGCRFNPRCPHAMEICRQKDPPEFYVGDGHTAACWLFEGGSEK"
     gene            complement(2029..3120)
                     /locus_tag="PKL93_09985"
     CDS             complement(2029..3120)
                     /locus_tag="PKL93_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71627.1"
                     /translation="MQSTKSRIAKNFFKHKLGVVGLIALSIMYLLVIFSDFIAPYNYV
                     TSHRNFVYAPPSKIRFFDEEGKWIGPFVYGMKKVRNPVTYRLEFSEDTSRPVPVKFFV
                     RGEEYRFWGIFKTDLHLFGVEESSDIAMVLLFGGDRFGRDLFSRVLIGGKVSMTVGLV
                     GTLISVFIGAIVGALSGFYGGTIDVVIQRFIELLRSFPRIPLWLALATILPPQWPSTW
                     VYFGIVIVLSLIGWMGVARVVRGMVLSLREKEFILAARVSGVSNMKIITRHLIPNTIS
                     YLIVVSTLSIPGMILGESAISFLGLGIKEPMTSWGLLLKQAQSISELEAHPWLMVPGI
                     FIIVAVLSFNFVGDALRDAVDPYRAVEKV"
     gene            complement(3122..4090)
                     /locus_tag="PKL93_09990"
     CDS             complement(3122..4090)
                     /locus_tag="PKL93_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71628.1"
                     /translation="MWSFIARRLLIMIPMMFFISVICFVITELQPGDFISQYLDNPRI
                     SPAQIELLRKNLGLDEPAHIRYWNWIKGIVTRGDFGYSFAFQRPVGELIWERMGWTVF
                     VALGAIVFQWILAVPMGIYSALHPYSFGDYSLTVIGFIGLSVPDFFLALILMYLMLQM
                     GATSVGGLFSPQFVGAPFSLEKFVDLLKHLWIPLVVVGMSGLAGLMRVMRGNMLDVIS
                     SPFVTSLRAHGLDEKTVKRHAIKNALNPMVSIAGTELPNVFSGTIIAAIVLNLPTMGP
                     FFYNALLNHDQYLVMTFLMFIAIITQIGNLFADIALAVLDPRIRMS"
     gene            complement(4147..5892)
                     /locus_tag="PKL93_09995"
     CDS             complement(4147..5892)
                     /locus_tag="PKL93_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71629.1"
                     /translation="MRRIFLLVLAVLFTAMLMAAPFVEDSMLTPDPNPRMGGTLYLAL
                     TGSPQSFNFYGTLDSNAYSVAGQMLTGLVEAHPVTSAIRPALAESWEVSEDNKEVTFH
                     LRDVKWSDGVPLTADDVIFTFEHFVMNPVARGNSVARFTIEAKPIEWVKVDDSTVKAI
                     LPAPYAAFFTVLSHAYIYPSHALESKIDKNRPDSVNDVWLTNTPLNEIPANGPFMLSE
                     YIIDQKVVMTKNPNYWKVDEYGNKLPYVDKIEYLVVSDAQVRLAKFMAGELDYMAVSG
                     NDFPILKERELAGGPFRVFLAEPTQPTPSPIHIAFNFDVENPKLREVFRTTEFRQAME
                     YALDRERIIDEVYNGLAVLGGVPVLPSNKAFYNPKIEEIRRPFNLAEAARILDELGLK
                     DNDGDGFREYPDGTKFEFVLTLQSSPQEYQDIAYIYSQDLQSIGVKVNLQILDSSLTG
                     QMFGAGSFEAGIRAFGNQPDPQLRKAIWQPGTQLYYWHRTTRDPDTNLPIMENMLDWE
                     KRVYELFEKGQVAMDPVERKSYYDEWQEIYAEYLPVIFVCKGMNLYAANKSLGNVEQN
                     EEGLLSYSSWTVFKK"
     gene            complement(5933..7048)
                     /locus_tag="PKL93_10000"
     CDS             complement(5933..7048)
                     /locus_tag="PKL93_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNQ71630.1"
                     /translation="MIWNEIRDLLSSGTRSILGVMSGTSADGLELAIVNCSGSGKSMK
                     VKLLEHSSVQFESSFHDEIVKTFDPSLSGVDRVNSMNFLIGKRHADAIKSFLNGTEVK
                     VDAIAYHGQTVYHDPAVHATLQIGEADIVSSETGLPVVFDFRKKDMVFGGEGAPIIPY
                     FDWIYFESGIYAVNLGGIANVSYVAEKLEDMKAFDTGPANCLIDLVAKRYFNMEFDEG
                     GKIAARGSVDSAILDVLIERDRRYFERQPPKSTGREIYNETFLEGISFSSPENLVRTL
                     VRFSVFQLVQSINSYLPLGKRIVVTGGGALNPVMIEDLKQLSGLDVEVPDKTFLQAKE
                     AIGMAVLAQEFFNGVEANVPSVTGARKRVVLGKLALP"
     gene            complement(7045..8064)
                     /locus_tag="PKL93_10005"
     CDS             complement(7045..8064)
                     /locus_tag="PKL93_10005"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNQ71631.1"
                     /translation="MIWNRVDEIVSCGIEKAVYPGAVVLVGKGTEVLYKNTYGFIAVD
                     DNTPTTFETVYDIASMSKVVATTSAIMILVSRGELSLWDTLGDFFDVPESKKAISIFH
                     LLTHTSGMQPYSEAWREFKGRELLKSIIDLEPETSPMEQINYSCLNFITLMAIVEKVT
                     SVKFDEFCSQQIFEPLGMNSTTYLPKSLDHVAPTSEREGKVLRGLPDDELAYYLGGVS
                     GNAGVFSSVEDLFKFVSSLMYARLVPSRVFETFISETVSIGESKRHLGWMAPARGSSA
                     GDMLGEEAFGHTGFTGTSIWVDPLTGLFVIMLSNRTNISRRDTIPQIQQIRRRLHNLV
                     FGSLG"
BASE COUNT         1955 a         2117 c         1958 g         2144 t
ORIGIN      
        1 cttacaactc acaactttga acttgcaact gatcctttgc tcttccaacc tctgactact
       61 gacctctaac ctcaggtttt atgcagccag caggcgactt tatgtccctc ggccacctca
      121 aagtttccgg gcatttcttc gctgcagatg tccattttgt aggggcatct agggtgaaac
      181 gggcagccag aaggtggatt tatcgggcta gggggctccc cttccagttt gatattctga
      241 agcttccggt cctcgggatc ccagtttgga atcgaagcca taagcgcctt tgtgtaaggg
      301 tgagcggggt tgttgaagag ttccctcgct tcggcagtct ctacgacgtt tccgagatac
      361 attaccatta ttctgtcgct catgtggtgt acgacgtcta agttgtgcga gatgaagata
      421 taggacatct cgctttcttc cctcagctcc tgcaacaggt ttatgatctg gctttgaacg
      481 gaaacgtcga gggcagatgt aggttcatca catataatca gtctcggtct aaccgagatc
      541 gaacgggcta tcgcgatcct ttgcctctgt cccccggaga actcgtgagg gtaacgctga
      601 gcgtattcgg gatagagacc gactttcatc agaagctctc caacgtaatc ttctgcttct
      661 tttttggata agagattgtg tgcctcgatc ggttcccgta taatatccct taccgtcatt
      721 ctggggtcaa gtgaatcgaa gggattctga aagatcatct gaacatctcg ccggaatttc
      781 gagcgcacgt tctttccaag ccctttggtt atgtcgtgtt cctctccatt ttcgtcatgg
      841 tagagtatct tcccaccggt cggctcgtag atctttacta tggtcctgcc cagagtactc
      901 ttgccgcagc ccgactcccc gacgactccg attgtctctc ccctgcttat ctcaagggta
      961 acagactgca ttgcccttac atgaccgact acctggaaga agacaccggc ctttatcgga
     1021 aagaacttct taagtccttc tgtagaaagt atcttcattt ttcggatccc ccctcgaaaa
     1081 gccagcaggc agcggtgtgg ccgtcaccaa catagaattc gggcgggtct ttttgtcggc
     1141 aaatctccat cgcgtgaggg catctagggt tgaatcgaca gccttctggg aattcatatg
     1201 ccctgggaac gtatccttca atgtaaggca gtttcttccc cttgaaggat cttgtcactc
     1261 tagagctgag aagacccttg gtgtacggat gcgagggcga ggaatatagt tccttcaccc
     1321 cggccttctc gacaatcttt ccggcgtaca ttacgtgtac tctgtcggcc agttcggcaa
     1381 ctaccccgag atcatgggtg atgaagatta ttgaactgcc gaattcaccc tggagctctc
     1441 tcataatgtt caggacctga gcctgaattg tgacatccag tgcggtggtt ggttcgtccg
     1501 ctattattat ctccggattt gttattaagg ccatcgcaat cataattctt tgaagcattc
     1561 cgccgctcat ttcgtgggga tactctctgt acctcttctc cggttctggg atatttaccc
     1621 tcctgagaat tgaaatgatc tcatcgtagg ttctggtctg gtcccagttc atgtgtgctc
     1681 tggcaacttc cgttagctgg aaccctatcg tgtacagggg atcgaatgcc gacataggtt
     1741 cctgaaagat catactgatt tccttgcccc ttatgtccct gtacgccttg ttagaaagtc
     1801 ccacgaggtt ctttcccttg tacaagatct ctccctcaac cttggccggc ggcactttta
     1861 tcagaccgag aatcgtctgg acggtaacgc ttttaccaca gccggattct ccgacaagtg
     1921 cgaggatctc tcccttctcg agatagaagc ttatgtcgtc cacagctttg accagaccgt
     1981 ccggggtcct gaaataagtt ttgagattct tcacctcgag tatcgcgctc ataccttctc
     2041 caccgccctg taaggatcga cggcgtctct caaggcgtct cccacgaagt tgaaggagag
     2101 aaccgcaacg attatgaaga ttcccggaac cattagccag ggatgggcct caagttcaga
     2161 tatagactgg gcctgtttca acagcaggcc ccagcttgtc ataggttcct ttataccgag
     2221 ccccaggaag ctaatcgcgc tctctcccag aatcattccc ggtattgaaa gagtagagac
     2281 gactatgagg tagcttatcg tgttaggaat caagtgacgc gttattattt tcatgttaga
     2341 tacaccggat actctggcgg ccagaatgaa ctccttctct ctcaggctca ggaccattcc
     2401 tctgacaaca cgcgcaacac ccatccagcc gattagtgaa agaacgatta cgattccgaa
     2461 atagacccat gtacttggcc attgaggagg tagaatcgtg gcaagcgcga gccagagggg
     2521 aatcctcgga aaggatctca gaagctcaat gaaacgctgt atgactacgt ctatagtccc
     2581 gccgtagaat cccgaaagcg ctccaactat cgctcctatg aatacgctta tcagagtgcc
     2641 gacgaggccg actgtcattg agaccttccc cccgatcagg accctggaga atagatccct
     2701 gccaaatctg tcgccgccaa aaagaagaac catcgctata tcggaagatt cttcaacacc
     2761 gaagagatgg agatcagtct tgaaaatccc ccagaatctg tattcctccc ctctgacgaa
     2821 gaacttcaca ggaactggcc ggctggtgtc ttcggagaat tccagcctgt aagtcacggg
     2881 gtttctgacc ttcttcatcc cgtacacgaa ggggccgatc catttacctt cttcatcgaa
     2941 gaatcggatc ttcgaggggg gcgcatagac gaaattcctg tgggaagtca catagttgta
     3001 tggcgctatg aagtcggaaa atatgacgag cagatacatg atagaaaggg ctattaagcc
     3061 aacaacacca agtttatgtt tgaagaaatt ctttgcaatc cggcttttcg tgctctgcat
     3121 atcagctcat ccttatccgt gggtcgagaa cggcaagcgc tatgtcggca aatagattcc
     3181 cgatctgggt tatgatggcg atgaacataa ggaacgtcat tactagatac tggtcgtggt
     3241 tgagaagcgc gttatagaag aacggtccca tcgtcggcag attcaggacg atagcggcta
     3301 ttatggtgcc gctgaatacg ttaggaagct ccgttccggc tatgctgacc atcggattga
     3361 gtgcgttttt tatcgcgtgc ctttttacgg tcttttcatc gagcccgtga gccctgagcg
     3421 aggtgacaaa gggagaagaa atgacatcta acatgttccc gcgcatgaca cgcatgagtc
     3481 cagcaagacc gctcatgcct actacaacga gaggaatcca caggtgttta agtagatcca
     3541 caaacttttc caggctgaaa ggcgccccaa cgaactgtgg cgaaaagagg ccaccgaccg
     3601 aggtcgcccc catctgcagc atgaggtaca taaggatcag tgccaggaag aagtccggta
     3661 cagaaagacc tatgaagcca ataactgtta gcgaatagtc accaaatgaa tatggatgca
     3721 gcgcagagta gattcccatc ggaacggcaa ggatccattg aaaaacgatg gctcctagtg
     3781 caacgaagac cgtccagccc attcgttccc atataagctc tccaacaggt ctttgaaagg
     3841 caaaggagta gccgaagtcc cctctggtga ctatcccctt gatccagttc cagtatctta
     3901 tgtgggccgg ttcatcgagc ccgagattct ttctgagtag ctcgatctgc gccggtgaaa
     3961 ttctcgggtt gtccagatac tggcttatga aatcaccggg ttgtagctcg gtgatcacga
     4021 agcagatcac ggatatgaag aacatcattg gaatcattat aagcaatctt ctagcgataa
     4081 aagaccacat aatcttcctc cggagaagta aagcgggagg agaatctcct cccgcagatg
     4141 aagcacttat ttcttaaata cggtccagga agagtaagag agaagcccct cttcgttctg
     4201 ttcgacgttt cctagcgact tgttcgcggc gtaaagattc attcctttgc acacgaaaat
     4261 cacgggaaga tactccgcgt agatctcctg ccattcgtcg taataggatt ttctttcgac
     4321 cgggtccatt gcgacctggc ccttttcaaa gagttcgtac actctctttt cccaatcaag
     4381 catgttttcc atgataggaa gattggtgtc ggggtctctc gttgttctgt gccagtagta
     4441 gagctgagtt cccggctgcc agatcgcctt tctcaactgg ggatcgggct ggttcccaaa
     4501 ggccctgatt ccagcttcga aactgccggc gccaaacatc tgtccagtta gcgagctatc
     4561 gagtatctgg aggttgacct ttactccgat actctgcaag tcctgagagt aaatgtatgc
     4621 gatgtcctgg tactcctgcg gagagctctg cagggtaagc acgaactcga acttcgtccc
     4681 gtcgggatac tctctgaagc cgtctccgtc attgtctttg agtccaagct cgtctagaat
     4741 tctcgcggcc tctgccaggt tgaagggtct gcgaatctct tcgatttttg gattgtagaa
     4801 ggccttgtta gagggaagta caggcactcc tccaagaacg gccagtccat tgtagacctc
     4861 atcgatgatc ctctctctgt caagggcata ttccattgcc tggcggaact cggtagttct
     4921 gaaaacctct ctgagtttcg ggttctcaac atcgaaattg aatgcgatgt gaatcgggct
     4981 tggagtaggc tgagtaggct cggcaaggaa gactctaaat ggaccgccgg caagttccct
     5041 ctccttgagg atcgggaagt cgtttccact cacggccatg tagtcgagct ctcctgccat
     5101 gaacttggca agcctcacct gtgcatcgct tacaacgagg tactcgatct tgtccacgta
     5161 aggaagcttg ttgccatatt catcgacctt ccagtagttt ggatttttcg tcatgacgac
     5221 cttctggtct attatgtatt ccgagagcat gaagggtcca tttgctggaa tctcgttcag
     5281 aggagtgttt gttagccata catcgttgac cgagtcgggt ctgttcttgt cgatcttgct
     5341 ttcaagcgca tgggacgggt agatataggc gtgcgataga actgtgaaga aggcagcgta
     5401 cggcgcgggc agaatagcct tgacagtgct gtcgtcgacc tttacccact caattggttt
     5461 tgcttcgatt gtgaatctgg ctacggagtt tcctctggct acgggattca taacgaagtg
     5521 ctcaaatgtg aagattacgt catcggcggt cagagggacg ccatcagacc acttcacatc
     5581 tctcaggtgg aaggtgacct ccttgttgtc ttccgagact tcccacgact ctgcaagggc
     5641 gggcctgatt gccgacgtaa ccggatgagc ctcgacgagt cctgtgagca tctgtcctgc
     5701 aacggagtaa gcgttgctat cgagagtgcc gtagaaattg aatgactggg gggaaccggt
     5761 aagtgccaag tacagcgtcc ctcccattct ggggtttgga tcaggtgtca gcatgctgtc
     5821 ttccacgaat ggagctgcca ttaacatggc ggtgaaaaga actgcgagaa ctaacagaaa
     5881 gattcgcctc acggtaaaaa cctcctttat gttttctctc ccgtcgggga gatcaaggta
     5941 aagccagttt tcctaggaca actctctttc tcgctcctgt tacagacgga acgtttgcct
     6001 ctaccccgtt aaagaactcc tgggctagaa ctgccatgcc aatcgcttcc tttgcctgca
     6061 agaaggtttt gtcaggcact tctacatcaa ggccgctaag ctgtttaaga tcctcgatca
     6121 taacggggtt aagggcccct cctcccgtaa ctactattct tttccccaaa ggtaaatatg
     6181 aattgatgct ttgaaccaac tggaatacgc tgaacctaac gagtgttcta acaaggtttt
     6241 ccggagagga gaaggagatc ccctccagga aggtttcgtt gtagatctct cttcccgtcg
     6301 attttggggg ctgcctctca aaatatcttc ggtctctctc tatgagaacg tcgagtatcg
     6361 cgctgtcgac tgagccccgg gcggcgatct tgcccccttc gtcaaactcc atgttgaaat
     6421 acctttttgc tactaaatct atcaggcagt tggcgggacc tgtgtcgaag gctttcatgt
     6481 cttcaagctt ctcggccaca tagctaacgt tcgctatacc acctaaattt actgcgtaga
     6541 tgccgctttc aaaatagatc cagtcgaaat aaggaatgat aggcgctccc tcacccccga
     6601 agaccatgtc cttcttcctg aaatcaaaga ccaccggaag gccagtctcg gacgacacga
     6661 tgtccgcttc accaatctga agagtggcat gcactgcggg atcgtggtaa acggtttgtc
     6721 cgtggtaagc gattgcgtca accttgactt ccgttccatt tagaaaagac ttgattgcat
     6781 ccgcatgtct tttcccgatg aggaagttca tcgaattgac tctgtccaca ccagaaagcg
     6841 aagggtcgaa agttttcact atttcatcgt ggaaactcga ctcgaactga acggaagagt
     6901 gctcgagaag cttcaccttc atggactttc cgctcccgga acaattaaca attgcaagct
     6961 ccaaaccgtc ggccgacgtt cccgacatga ctccgagaat gcttcttgtt ccgctggaga
     7021 gaagatctct tatctcattc cagatcatcc gaggctcccg aagacaaggt tgtgcaggcg
     7081 tcttctgatc tgctgaatct gcggtatcgt atcccgcctg ctgatattgg ttcggttgga
     7141 cagcataata acgaatagtc ccgtaagtgg atcgacccag atcgacgttc cggtaaagcc
     7201 ggtgtgtcca aaagcctctt ctccaagcat gtcacctgca ctggaacctc ttgcaggtgc
     7261 catccagcct agatgccgtt tgctttcacc tatcgatact gtttcagata tgaacgtttc
     7321 aaagaccctg gaaggtacca gtctagcata cataagactg gagacgaact tgaaaagatc
     7381 ttcaaccgat gaaaaaacgc cggcatttcc gctcactcca ccgagataat acgcaagttc
     7441 gtcatccggc aaccctctta gaacctttcc ttcccgttcg cttgtcggcg ccacgtgatc
     7501 taagctcttc ggcagatatg tggtcgaatt catcccgagc ggttcgaaaa tctgttgtga
     7561 acagaactcg tcgaatttca cagaagtgac tttctctaca atggccatga gagttatgaa
     7621 gttcaggcat gaatagttta tctgttccat cggcgaggtt tcaggttcga gatctatgat
     7681 tgatttcagc aattcacgtc ccttaaactc tcgccatgcc tcggaatatg gctgcatacc
     7741 tgaagtgtgc gtgagcagat ggaagatcga tatcgccttc ttcgactccg gaacatcgaa
     7801 gaaatctccg agcgtatccc aaagagaaag ctccccgcgc gaaacgagaa tcattatcgc
     7861 gcttgtggtt gcaaccacct tgctcatgct tgcgatgtcg taaacagttt caaaagtcgt
     7921 gggggtattg tcgtctaccg caatgaagcc ataggtattc ttgtaaagga cttcggtacc
     7981 ctttcccacc aaaactacgg ctcccgggta cactgctttt tcaataccac acgagactat
     8041 ttcatcaacc ctattccaga tcaaagcgag cctccttatg tattacttag tccgtaaacg
     8101 gtctaccgtc ctccgagaag accaagtcgt ccttggtcct tcgtcccaaa ccatgaaccc
     8161 gtccttcggg aaga
//
LOCUS       DAOQYI010000064         7980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_9514_length_7980_cov_74.255268, whole genome shotgun sequence.
ACCESSION   DAOQYI010000064 DAOQYI010000000
VERSION     DAOQYI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9514_length_7980_cov_74.255268"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1352)
                     /gene="rlmD"
                     /locus_tag="PKL93_10010"
     CDS             complement(33..1352)
                     /gene="rlmD"
                     /locus_tag="PKL93_10010"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNQ71632.1"
                     /translation="MEELRIEKLIAGGFSLARTADGKVALLDAGYPGEVVEASKKMGR
                     SDFHTMKVERVLVPSNSRRERLCRNFPVCGGCDWQTLNYSEQLFWKREVIREQFKRVG
                     KIELEDFEIVPSPRETNYRLKMEYVCHRGKDGLSLGLYRRNSNSPVSCQGCVLGLKDF
                     EKARSGFEEILRKTSIMAYNRVTGKGELKHVILRGNGREIMAILVTKGEYLPDENRIV
                     FEVKKRLPYVSTLVHLMNANDRVVMRGVSRTLFGEGVIDQELSGRKFEIPPVAFFQNN
                     LEVTESIVSHLSNEMKDTACDSLLDLYSGIGTFSITVGKKMGSVTAVESNPVSVKALK
                     ANSNLNGMFGIEIVSSDVIEFLKSDRRHYSTIILDPPRSGVGSEIGLLDKFGASMVFY
                     VSCDPTTLARDTAKLRESGYKISSVKAFDMFPQTWHVETVVCLVKKR"
     gene            complement(1352..2983)
                     /gene="argS"
                     /locus_tag="PKL93_10015"
     CDS             complement(1352..2983)
                     /gene="argS"
                     /locus_tag="PKL93_10015"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNQ71633.1"
                     /translation="MFKEKLENSIKKVLEEFDIYDDKIDFKVEIPPEGFGDLSTNAAF
                     ILSRILKKSPREIANIICERLGKETDYSRVEVAGPGFINVDFSITYLSSVLAELIDAP
                     DFWKRKGDTRIQFEFASANPTGPFTVGHGRQAVFGDVLCRIFSSRGYRVQREMYINDA
                     GRQIGLLGRSLWVRYNQYLGVEEELPEDGYQGEYLIDIARGLAGEIGERFKAVWNSEA
                     EAFFKKYALDKMLEDIMGTLKRLRVEFDNVFFESSLIDDGTVDSVIGMLDRKELIYES
                     EGARWLKVSNFVEDDDKVLVRSNGSYTYFMTDIAYHYNKHNRGFKKVFDIWGADHMGH
                     IPRMNAAMKALGIEDGFLNVIVHQYVNLKREGEVVKMSTRRGEFTTLDELVEAVGVDS
                     TRYFFAMFDPDTHMLFDIDLARQRSNDNPVFYVQYANARISNVFRTAKEKNVKRSRDS
                     LELLDSQEDRRIIKLLTIFPEILDSVVADYRTNRLTSYLEDLSRAFHGYYNKNIIVDP
                     ENPALSGARLALCEAIQNILRSGLELLGVEAPDSM"
     gene            3147..4088
                     /gene="argF"
                     /locus_tag="PKL93_10020"
     CDS             3147..4088
                     /gene="argF"
                     /locus_tag="PKL93_10020"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNQ71634.1"
                     /translation="MGVNLKGKSFLTLLDFTTDEIRYLLDIARQVKVEKRAGTPTSRF
                     AGRTLAIVFEKRSTRTRTAFETSFGEEGGHAVFLSKDDIHLGVKEDLKDTARVLGRMF
                     DAIAFRGFKQETVEVLAQWSGVPVYNGLTDLYHPTQILADFMTIEESLGSLKGRKLVF
                     VGDSRNNMANSLMIGSAKMGLHFVACGPESLHTDPAIVSKCKEIAKQSGATVEVTEDI
                     DSALKDADAVYTDVWASMGEEDKLQERIALLKPYQVNAEMMKKTGKAETIFLHCLPAV
                     KGNEVTEDVFESPASRVFEEAENRKHTIKAVMIATLL"
     gene            4097..5035
                     /gene="arcC"
                     /locus_tag="PKL93_10025"
     CDS             4097..5035
                     /gene="arcC"
                     /locus_tag="PKL93_10025"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNQ71635.1"
                     /translation="MKRIVVAIGGNALNKPNEKPTAEVMEKNLLSTTAYLADLVEEGY
                     ELVITHGNGPQVGNLLVQQDIAKDVFPPFPIDVNDAMTQGSIGYLITQTLNNELVKRN
                     TRKNIACVLTQIVVDKNDKGFENPSKPVGPFYDRETAKELERTKGWTVKEDAGRGYRR
                     VVPSPMPLDVLEIEPIREMIKDGTIVVAAGGGGIPVVRDESGRIKGVEAVIDKDRASA
                     LLAKLIEADALVILTGVDYAYINFGKDNQRELKELTVKEGRELMEAGHFAKGSMLPKI
                     ESAIEFVSLTGKEAIITSLENVDLALKGESGTRIVA"
     gene            5130..5681
                     /locus_tag="PKL93_10030"
     CDS             5130..5681
                     /locus_tag="PKL93_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ71636.1"
                     /translation="MRGQETRERIMEAAIQVIAEETIEGLSTRKVSGIAEVNLAAIHY
                     HHRSKEGMLIDLSRHILNNYVLPRIEPLIDQGKQPVEITRDLYAELLKLYSERPDIMV
                     SLVYLWLHGMKNKRVREILINARADFYDRLERALGSSMSMRRASKVSHRLLSYIAGKI
                     MEMAVDGEMPDEKEVDEIAKLLS"
     gene            complement(5749..6396)
                     /locus_tag="PKL93_10035"
     CDS             complement(5749..6396)
                     /locus_tag="PKL93_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71637.1"
                     /translation="MKRVYIILTVFFLIAFVSFGSMHSSRMMTPGSVRAMAGFKDIGL
                     RIGILSFLEAGWFTDEGPYFTVGYDKNFHFASRVSFSSLQEARVIAELGYDFNVVYLE
                     VGGLYEYAAPDASFLGGQLKAEVFFDNDLSIVNATLGRFQKVAGETQKQSYTSIGFSA
                     RRKLEIGKQFYFINLESVELVGTLKWEIEDDLSSFSPFPAYSFVGIQLNLGFLSQ"
     gene            6473..7564
                     /locus_tag="PKL93_10040"
     CDS             6473..7564
                     /locus_tag="PKL93_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNQ71638.1"
                     /translation="MKKTKFWVLGYFIAVFMTLFLFPTTSRVLGLAFIFSLIINTPAQ
                     FVSRKIPRRALSIITGIALLSFMFFLIYRVVPITINGVKGLSLELDSLFNGGTFDKWL
                     EKLPPFISEAIVDIVESASQWLSDAAIMIGQYVASNITSWITGVVLLIIAGFVIARKT
                     NMIQKSVPVLFPGCDQDKVSSFFHSLYKDFQTYIGGQLIIALVVGTIIAVGSAILGVP
                     NAIFLGLLAGITNFIPYLGVVVTSIPMIVLSFVSKGLWGVVGAIVLLVFANQMEIWFL
                     SPKILSNRVKINWFIVLVSIIGFGELLGVFGVVFAVPILIFIKRYWMDFILSERSGSN
                     WESTIETGIKKAKIRKEHHPEEPTGSQED"
     gene            complement(7693..>7980)
                     /locus_tag="PKL93_10045"
     CDS             complement(7693..>7980)
                     /locus_tag="PKL93_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71639.1"
                     /translation="FVYEYFKQGEVTLKTPYLNMEEPEDDPGEFTAPEDWGGPVGETV
                     NANILKLNVMIPYRDFTFGSDFSLYFGDYFKKNGGYERPIFEIRPYMSYVF"
BASE COUNT         2252 a         1857 c         1755 g         2116 t
ORIGIN      
        1 agctatgtga agcaaactcc actcggtcga ctttatcgct ttttgacaag acacacaact
       61 gtttccacgt gccatgtctg gggaaacata tcgaaagcct ttaccgaaga gattttgtag
      121 cctgactctc tgagctttgc ggtatctctc gccagggtag tagggtcaca ggacacataa
      181 aacaccatgg aagcgccgaa cttatcgaga agcccaatct ccgaaccgac tccggatctg
      241 ggcggatcca gaattatcgt agagtagtga cgcctatcgg acttgagaaa ttcaataaca
      301 tcgctcgaca cgatttcgat cccaaacatt ccgttaagat tcgaatttgc cttgagagcc
      361 ttaacagaca cgggattcga ttctaccgcg gtgacactac ccatcttctt cccaacggtg
      421 atagagaatg ttcctatgcc gctgtaaaga tcaagaagag agtcacaagc cgtatccttc
      481 atctcattag atagatgact gacaatcgac tccgtaactt cgagattgtt ttgaaagaag
      541 gctacgggcg gaatctcaaa ctttcttccg gaaagctcct ggtcgattac cccttccccg
      601 aaaagcgttc tagaaacgcc tctcataact accctatcgt tcgcattcat aagatgaacg
      661 agtgtcgaga catagggaag cctcttctta acctcgaaga caatacggtt ttcatcggga
      721 agatattctc cttttgtaac gagtattgcc atgatttctc ttccgttgcc tcttagaatt
      781 acatgcttga gttcgccttt tccagtaacc ctgttatatg ccatgattga agttttccta
      841 agtatctcct cgaaacccga tctggccttt tcgaagtctt tcaggcccaa cacacaaccc
      901 tggcaactaa cgggagagtt gctattcctc ctataaagac cgagagaaag cccgtctttc
      961 cccctgtgac acacatactc catcttaagt ctgtaattcg tctctcttgg cgaaggaaca
     1021 atctcaaagt cctccagttc aatctttcct actcttttga actgctccct aatcacctct
     1081 ctcttccaga aaagctgttc tgaatagtta agtgtctgcc agtcgcatcc gccgcatacc
     1141 ggaaagttcc tgcaaagcct ttctctccgg ctatttgaag ggacaagcac tctctcaacc
     1201 ttcatcgtat ggaagtcgct tcttcccatt ttcttgctag cttcaacaac ttcgcctgga
     1261 taacccgcat cgagcaaagc tactttcccg tcagctgttc tggcgagaga gaaacctcct
     1321 gcgattagct tctcaatacg aagttcttcc attacatact atcgggagcc tcaacgccaa
     1381 gaagctctaa tccgctcctt aaaatgttct gaatcgcttc gcacaaagct aaccttgcac
     1441 ctgaaagtgc cgggttctcc gggtctacaa tgatattttt gttgtagtaa ccgtgaaatg
     1501 ccctggacag atcctccaga taagacgtta gacggtttgt tctgtaatct gcgactaccg
     1561 aatccaaaat ctcaggaaaa atcgtcagca gcttgatgat cctcctgtct tcctgagaat
     1621 cgagaagctc aagcgaatct ctcgatcttt tcacgttctt ctccttagca gttctgaata
     1681 cattgcttat tcttgcgttg gcatactgga cgtaaaagac aggattgtcg ttcgatctct
     1741 gcctcgccag atctatgtca aagagcatgt gagtgtcagg atcgaacatc gcgaagaagt
     1801 atctcgtcga atccactcca acagcttcaa ccagctcatc cagggttgtg aattcccctc
     1861 ttctggtcga catcttcacc acttcaccct ctcttttgag atttacgtac tgatggacaa
     1921 tcacattcag aaatccatct tctattccca gggccttcat agcagcattc atcctaggaa
     1981 tatggcccat gtgatccgca ccccagatat caaatacctt tttgaaaccc ctattgtgct
     2041 tattgtaatg ataagcaatg tcggtcatga agtaagtata gcttccgttc gatctcacga
     2101 gaaccttatc atcatcctcc acaaagtttg agactttaag ccacctcgcc ccttccgatt
     2161 catatatcaa ctcttttctg tcaagcatgc caataacaga gtcaacagtt ccatcgtcta
     2221 tcagagagct ctcaaagaag acgttatcaa actccactct cagcctcttg agtgttccca
     2281 taatatcttc gagcatcttg tcgagagcat atttcttgaa aaaagcttcg gcctctgagt
     2341 tccaaaccgc cttgaaacgc tctccgatct caccggcaag tccccgagcg atatcgatca
     2401 aatactcacc ctgatagcca tcctcaggta gctcctcttc tacaccgagg tattgattgt
     2461 atctaaccca caaagagcgg cccaggagtc caatctgcct tcccgcgtca tttatgtaca
     2521 tctctctttg gactctgtat cctcttgagg aaaagattcg gcacaaaacg tctccaaata
     2581 ctgcctggcg accgtgaccg actgtgaagg gtcccgttgg gttagcactt gcaaattcaa
     2641 actgaattct cgtatcgccc tttctcttcc agaaatccgg cgcatcgatc aactccgcca
     2701 gtacagagga tagatatgta atggagaaat caacgtttat gaatcctggt ccggcaacct
     2761 ccactctgct atagtcggtt tctttgccga gtctctcgca gattatatta gcgatttctc
     2821 tcgggctctt cttcaagatc ctcgaaagaa taaacgcggc atttgtagaa agatcaccaa
     2881 acccctcagg aggtatctca accttaaaat cgattttgtc atcataaatg tcaaattcct
     2941 ccagcacttt ctttattgag ttctccagct tctccttaaa cacaatccac ctcctaatct
     3001 ggccaatata ccttagcata caaaagcata acaccgcatt gttgtgatat ggtttaatct
     3061 gcgtttcctc gtacgaataa acgcaattga aagcatagtc ttatatatgt attatattag
     3121 agatgactaa aacaaggagg gttaagatgg gcgtaaacct taaaggaaag agctttttga
     3181 cactgcttga tttcacgaca gacgagattc gctatctact ggatattgca agacaggtga
     3241 aggtcgaaaa gagggccggt acaccgacaa gcagatttgc agggagaacg cttgcaattg
     3301 tattcgagaa gcgctcaaca aggacaagaa ctgctttcga aacttccttc ggtgaagagg
     3361 gcggccatgc ggtctttctc tcaaaggacg atattcatct tggagtgaaa gaggatttga
     3421 aagacacggc aagagtacta ggaagaatgt tcgatgcgat agcgttcagg ggcttcaagc
     3481 aagagactgt tgaagttctg gcgcagtggt caggcgttcc agtctataac gggcttaccg
     3541 acctatacca tccaacccaa attctagctg atttcatgac tatcgaagaa agcctcggca
     3601 gcctgaaagg acgaaagctg gtctttgtgg gggattccag aaacaacatg gctaactccc
     3661 tcatgatagg gtccgcgaag atggggttgc atttcgtagc gtgcggtccg gagagccttc
     3721 atactgatcc ggctattgtc agcaagtgca aggagatcgc aaaacaatcc ggagctacag
     3781 tcgaggtcac cgaagatatc gactctgcac taaaggacgc cgatgcagtc tatacagacg
     3841 tctgggcatc gatgggtgaa gaggacaagc ttcaagaaag gattgctctg cttaaaccct
     3901 atcaggttaa cgctgagatg atgaaaaaga ccggaaaggc agagactatc ttccttcact
     3961 gtctgcctgc cgtgaaggga aatgaagtaa ccgaagatgt cttcgaaagt cctgcctcta
     4021 gagtcttcga ggaagccgag aacagaaaac atacaataaa agcagtaatg atagccacac
     4081 tgctataggg ggaagtatga agagaatcgt agttgcaata ggcggcaacg cgctcaacaa
     4141 accaaacgaa aaacctacag cagaagttat ggagaagaac ctgctctcga cgactgccta
     4201 tcttgcagat ctggtagagg aaggttatga gcttgtaatt actcatggca atggaccgca
     4261 ggttggaaat ctccttgttc agcaggatat cgcgaaggat gttttccctc cctttccgat
     4321 cgacgtaaac gatgcaatga cacagggctc aattggttac ttgataaccc agactttgaa
     4381 caatgaactt gtcaagagaa acacaagaaa gaatatagcg tgcgttctga ctcagatagt
     4441 tgtggacaag aacgacaagg gtttcgagaa tccttccaaa cccgtcggac cgttttatga
     4501 cagagagacg gccaaagaac tcgagagaac caaaggctgg accgtcaaag aagatgccgg
     4561 aagaggttat cgacgtgtag taccttcacc tatgcccctt gacgttcttg aaatcgaacc
     4621 tattagggag atgataaagg acggcaccat cgtcgtggcg gctggcggcg gtggaatacc
     4681 ggtggtcaga gacgaatcgg gacgaattaa aggcgtcgaa gccgtcattg acaaagacag
     4741 agcttctgcc ctgctggcga agctaattga agcggacgca cttgttatcc ttacaggagt
     4801 agactacgcg tacataaact ttggaaaaga caaccagagg gaattgaagg aattgactgt
     4861 aaaagaagga agagagttga tggaagccgg tcactttgct aagggaagca tgttgccaaa
     4921 gatagaatcc gcaattgaat ttgtctctct aaccgggaag gaagcgatca ttacttccct
     4981 ggaaaatgta gatctcgcgc tcaaaggaga gtcgggcaca agaatcgttg catagacaga
     5041 agccggagag attctccggc tttttttgtt tttcctgtaa cgatgtgata taatctgtgt
     5101 tatacgaatg attgaaagga gtgacgacag tgcgcggtca agaaacaaga gagagaataa
     5161 tggaggctgc tatacaggtc atcgctgaag aaacgatcga aggcctgagc acacgtaagg
     5221 tttcagggat agcagaagtg aaccttgcgg ctattcacta tcaccaccgt tccaaagagg
     5281 gaatgctaat cgatctatca aggcatattc tcaacaacta cgtcctgccg agaatcgaac
     5341 cactcatcga ccaaggcaaa cagcctgtag agattacaag agatctttac gcagaactgt
     5401 tgaagctcta ttctgaaagg ccagatataa tggtttctct tgtatatctc tggcttcacg
     5461 gaatgaagaa caagagagtg agggagatcc ttatcaatgc tagggccgat ttctacgata
     5521 gattagagag agcgctaggg tcttcaatgt caatgagacg tgcttccaaa gtgtctcatc
     5581 gtcttctatc ttacatagca ggcaaaatca tggaaatggc agttgatggt gagatgcctg
     5641 acgaaaagga agtagacgaa atagccaagc ttctttcata gtttgatttc tgcggataat
     5701 ctatcaggaa aggcaacgta aaatcccttc cgattatcgg aagggtttct attggcttaa
     5761 aaatcccaga ttaagctgga ttcccacaaa actgtacgca ggaaaggggc tgaaggaaga
     5821 gaggtcgtct tctatctccc attttaatgt accaacaagc tcgacacttt cgaggttgat
     5881 aaagtaaaat tgcttcccaa tttccagttt tcgcctggcg gagaaaccaa tagaggtata
     5941 actttgcttc tgcgtctcgc cagcaacctt ctgaaatctg ccaagggtag cgttgacaat
     6001 gctcaaatca ttatcgaaaa acacttccgc cttcagttgt ccgccaagaa acgaggcatc
     6061 gggagcggcg tattcataaa gccctcctac ctctagataa acaacgttga aatcatatcc
     6121 taactccgcg attactctcg cttcttgaag cgaagagaaa gaaacgcgcg aagcaaaatg
     6181 aaagttttta tcgtagccca cggtaaagta agggccttca tcagtgaacc aaccagcttc
     6241 aagaaaggaa agaattccaa tcctcagtcc aatgtctttg aaaccagcca ttgctctgac
     6301 agatccagga gtcatcatcc ttgatgaatg catcgagcca aatgaaacaa atgcaataag
     6361 aaaaaagact gtcaggataa tatatactct tttcataaag gcacctcaga agatattata
     6421 catattgagt atacaacaga tttgtcacta ttctccggtg gagggcttta ttatgaagaa
     6481 aacaaagttc tgggttcttg gatatttcat agcagtgttc atgacactct ttctttttcc
     6541 tacgacctca agggttctcg gactggcctt tatattttcg ctaattatca acacaccggc
     6601 tcaatttgtc agtagaaaga tccccaggag agccctaagc ataataaccg gcatagctct
     6661 tctatcgttt atgtttttcc ttatctacag agtagttccg ataaccatca atggtgtgaa
     6721 aggcctttcg ttggagctgg attctctttt caatggcggt acattcgaca agtggctgga
     6781 gaaacttccc ccattcattt ccgaagcgat agtcgacata gtcgagagcg catcccagtg
     6841 gttgagtgat gcagcaatca tgattgggca atacgttgcc tcaaacatca cttcatggat
     6901 tacaggtgtt gtcttgctga ttattgcggg ttttgtgatc gcaagaaaga ccaatatgat
     6961 ccagaaaagc gtgcccgtac tttttcccgg gtgtgatcag gacaaggtga gcagcttttt
     7021 tcattcgttg tacaaggatt ttcagaccta catcggcggc cagctgataa ttgctcttgt
     7081 tgttggaaca ataatcgcag taggatcggc tattcttggt gttcccaatg cgattttcct
     7141 tggtttgtta gcgggaataa cgaattttat cccttatctt ggtgtggtag ttacgtcaat
     7201 tccgatgatc gtgctttctt tcgtaagcaa agggctttgg ggggttgttg gagccatcgt
     7261 cttgctggtc ttcgccaacc agatggagat ctggttcctg tcgccgaaaa ttctctcgaa
     7321 cagagttaag attaactggt tcatcgtgct ggtatcgata atagggtttg gcgaactgct
     7381 gggcgtcttt ggcgttgttt ttgcagttcc aatcctgata ttcataaaga ggtactggat
     7441 ggatttcatt ctcagtgaaa ggagtggtag taattgggaa tctacgatag agactggtat
     7501 aaagaaagcc aaaatcagga aagaacacca ccccgaggaa ccgacaggtt ctcaagagga
     7561 ttgattgtag gactgataat cggattccta ataggattcc ttgtatttgg cttcatatag
     7621 tcttgttaga aaaaagggac gggtcatttc ccgtcccttt ttataaaggc atttatagga
     7681 gtaacacact ttctagaaga cgtaggacat ataaggtctg atctcgaaaa tcggtctttc
     7741 ataccctccg ttcttcttga aataatctcc aaagtataga gagaagtcgc ttccgaacgt
     7801 aaagtccctg taagggatca ttacattgag cttcaagatg ttcgcattaa cagtttcacc
     7861 aactggccca ccccaatctt caggagccgt aaactcaccg gggtcgtctt ccggctcttc
     7921 catgttcaag taaggggttt tcagagtaac ttctccctgt ttgaagtatt catatacaaa
//
LOCUS       DAOQYI010000065         7959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_9550_length_7959_cov_73.608679, whole genome shotgun sequence.
ACCESSION   DAOQYI010000065 DAOQYI010000000
VERSION     DAOQYI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9550_length_7959_cov_73.608679"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..1172)
                     /locus_tag="PKL93_10050"
     CDS             complement(327..1172)
                     /locus_tag="PKL93_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNQ71640.1"
                     /translation="MPLVPLTEVLKDAQAKKYAVPGFNFHLYEDLVAIVEAAQEARSP
                     VILMAAGTCMKHWGPTLAAALIKEVADSVDIPVVAHLDHASSLELIFKSIHAGFTSVM
                     YDGSMLPVEENIANSKIVVKVARAFDVSVEAELGRVAKGEEGESAVEILTDPSDVVMF
                     CEATDVDALAVAVGTVHGMQKQEAKIHLDLVDAISEVSPVPLVLHGSSGVSDNDLRYI
                     ATTGFSKINIGTRLKTVFTEGIREVLQNDPDLNDQLKLLKLAVPRVKDTVKEKIELLG
                     SGNRA"
     gene            complement(1174..2631)
                     /locus_tag="PKL93_10055"
     CDS             complement(1174..2631)
                     /locus_tag="PKL93_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNQ71641.1"
                     /translation="MNVFLGIDIGTTNIKCLVLGEDGRILEVIHSSTPKNKERGVEFL
                     DLELTRSYVESIIGKVGTLHSLAAIAFSSFGETVIPVRQRKALSKPVMWYDRSTYPLW
                     ESHRESVDNLAPYRITGVENSYTFSLYKILLQKELFRPEGVENWLPVSSYLAYSLGGV
                     PAWDMSQACRSFMVNIHSRRWNSQLIKYIGESEETMGELRYTGEPVGHTKSGIPLISA
                     GHDHITGLYAARAFARGKEFLFDSMGSASVIAAVVSGTAESLDFAGPFMPGGTVGIAY
                     EDHQYYIESNVRYYGKLLQSLMDLTQTDATKEGYERLNNEIEKLGQLNSRPLFLVNGD
                     LVVGEGLQGITLLEMPIPLNKEQLMQSAYIYLSSISKRIVDNIEKITRKELPIISGGG
                     GSLNSLLMKYKASLLGKEIWVLPTSELTALGGAFAAAHGVGADEVIEKCVNLLEPEEI
                     HPDEHLVEPLKEIYEKNAKEYLAIERNKKLDIQEG"
     gene            complement(2628..3584)
                     /locus_tag="PKL93_10060"
     CDS             complement(2628..3584)
                     /locus_tag="PKL93_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ71642.1"
                     /translation="MKEDNNIDTCILNLNPCYDHWVILKNPTKIPNVVRGDKVVTLVD
                     GKGLNIARVLSKVLGHSEYFCINILGGQVGTIIEKECDRLGIVTENFWIEDSNRINTA
                     LVYEYENKMLMINEPGPLMKPGEIEGFMEFFKGHVRPGMNLVISGSAPRGFENGSLLQ
                     LVRIAREAGCSLKVDIAGSWLSKIVTVSPELLKINADELKVAFGINKDDFKSMDDFRR
                     EYSIRDLIITNGKMGSVWLSESEKLHARSMKVFSDFSVGSGDSFFAGLIYGQEAKMSK
                     REALKIATACGAANTMHYGAAIFEYSDVEKVIGDVVVTEVVL"
     gene            complement(3568..4536)
                     /locus_tag="PKL93_10065"
     CDS             complement(3568..4536)
                     /locus_tag="PKL93_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HNQ71643.1"
                     /translation="MISDELLFEVATDYYVKGMLQKDIANKYGVSRVQISKYLKMAQE
                     RGIVHIEVEQPSVKTSIRREYEDFFRERYGLKKMFIARGARNEKTVLKALSREVHKYL
                     RTLPEKPMNIGLGWGNTIFTVAESIERLERPDWQLIPLSGGTARLADKRFNINHIVQN
                     FATRLSGKAVPMYLPFIFENARQLENTKQSLEYMNIQKLWNSLDIIICSVGYSIARSP
                     LFRENLLEVSYADELEKNNVVGDVLTHYFDINGKRFEKSILDKCINLSFEQYMNAGER
                     VIVAAGHHKVDGLVGMLRGGFADVLITDEFTAKFVKEYIVYDEGGQ"
     gene            complement(4540..5376)
                     /locus_tag="PKL93_10070"
     CDS             complement(4540..5376)
                     /locus_tag="PKL93_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ71644.1"
                     /translation="MKRSTKKKVKKSITYSLLIFLSVVIAFPFVWLILTAIKTYPDIY
                     AYPIKYFIFEPTREHFDKIADMNFWSYFKNSVIVGTGTMFFSILIGLFPAYAFARYEF
                     RFKRPLLTGVMVFQMLPVVVFLLPIFKTLNNIGILNTYFGLILSYLPFTTPISIMFMR
                     TFFLSIPKSLEEAARIDGCTFGQAFRKVILPITLPGIAAVGVYAFLFSWSELMYSMSI
                     LTSKAKQTIPTFLQLFVGQYQTRWGPLFAGSILATIPPLIIFMILQKFFIAGLVSGSV
                     KE"
     gene            complement(5373..6239)
                     /locus_tag="PKL93_10075"
     CDS             complement(5373..6239)
                     /locus_tag="PKL93_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ71645.1"
                     /translation="MSKLAREEKTAFKMVLPYLLIIVLLFVYLIGSNIYSSFTTEEGA
                     FTLQNYSNVFTDPVVGRSMINTVVWVIGSVLGQLLLGLLVALLLNESTKGQIIFRSII
                     LILPWATLDIVAGVMWKWMYNDMYGVLNDVLVKMNIINDYIPWLATENMAMISVIIAN
                     IWKGFCLSGMFFLAGLQTIPPDLYEAAEIDGANSIRRFWRITIPQLKPVIMTTLMLTV
                     IWTINYFPLIYIMTGGGPGYGTETVVTYIYKLGFRFLEFNKAAALSNILFVAIFAIAF
                     VFLRSIAKEEAR"
     gene            complement(6307..7548)
                     /locus_tag="PKL93_10080"
     CDS             complement(6307..7548)
                     /locus_tag="PKL93_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ71646.1"
                     /translation="MRKSTLLVFMMLVVLAFTGVSQVINIWIGGQVAELDETWEMIVG
                     KFEEETGLKVEVQLFGFDIYYDKLLTALQAGQGPDLAFADLGGWVPTFAEKGWLEPMG
                     ALLDSWDGTEQIWPNLWPTVLHNGERYGLPWYTDCRLLLYNQTMFDQAGLDRNDPPAT
                     WEELVYQAMKLTDASKRVFGYGVSGTKTEHTTLAYIIFLYGAGGQLLTDDYSAAAFNT
                     PEGLRALKFYTDLALTYAVSPNAVTYNEDDYRNMMAQNRVAMAVGGPWSFPLIETANP
                     DIVGNYSVSLHPYGATPASVLGGWALVIPSASKNKDNAWELAKYLTSYETWMTWIEAK
                     GGPMPSRQDVCFDSPVLQDPKWKVIFETFPHAVSRPPIPEYPQVSEQIQIMIQDVLLG
                     KVTPEEAIKTAEANVNAILAK"
BASE COUNT         2052 a         1950 c         1700 g         2257 t
ORIGIN      
        1 cgagactggc caggcggagc ctgactggcc tgcgttagca gactggctcc caaatgttcc
       61 taaggtccca ttttcgcggg aatttgtcag gtcctagaag aggcagaata acgcgaaaca
      121 agagaagact gtcatcctaa gtcgctcaat tctgcctctt acttgtcatc ctgtgaagcc
      181 tgccctgaaa tgctattgtt cagggttcct gtttgggggc tcctgtccaa ggggttctgt
      241 tcagggcatc tgcacgggat tgcagtcttg ctagacccat accaaaccca cttctcaacg
      301 tagcactgat ttagatcagg cgataactag gctctgttgc cgcttccgag gagttctatt
      361 ttctctttta ctgtgtcctt gactctcggc acggccagtt tgagtagttt cagctgatca
      421 tttagatctg gatcattctg tagaacctct cttatacctt cagtaaagac ggtcttcagt
      481 cttgtcccga tatttatctt ggagaaacca gttgtggcaa tgtacctaag gtcgttgtcg
      541 gatacgccgc tggaaccatg cagcacaagt ggtacaggtg agacttcgga tatcgcgtcg
      601 accagatcta gatgaatctt ggcttcctgc ttctgcattc cgtgcacagt gcccacagcc
      661 acggcaagcg cgtcgacgtc ggttgcctcg cagaacatta ctacatccga aggatcggta
      721 agtatttcaa cggccgattc cccttcttca ccctttgcaa ctcttcccag ttcagcttca
      781 actgatacat cgaaagctct tgcaaccttg actacgatct tgctgttggc aatgttttct
      841 tcaacgggta acatggagcc gtcgtacata actgaagtga accctgcgtg gatggacttg
      901 aatatcagct caagactact agcgtgatcg agatgggcaa caaccggtat atcgacactg
      961 tccgccactt ctttaataag agccgctgca agcgtcggcc cccagtgttt catacaggtt
     1021 ccagcagcca tgagtattac tggtgatctc gcttcctgcg ctgcttctac aatcgcaacg
     1081 aggtcttcgt atagatgaaa gttgaaacca ggcacagcgt atttctttgc ttgtgcatcc
     1141 ttgagaactt ctgtaagcgg tactaacggc attttatccc tcctgaatgt ctaacttttt
     1201 gtttctttct attgccagat attctttcgc gttcttctca tagatctctt tcagcggctc
     1261 aactagatgc tcgtccgggt gaatctcttc cggttcaaga aggtttacgc atttttcaat
     1321 aacctcatcg gctccaacgc catgagccgc tgcaaaagct ccacccagag cggtcaattc
     1381 gcttgtaggc aaaacccaga tctcttttcc gagcaatgaa gccttgtatt tcatcagcag
     1441 gctattgagg cttcctcctc ctccgctgat gattggtagc tcttttcttg taatcttctc
     1501 aatgttatcc acgattcgct tgctgatcga ggaaaggtat atgtaagcag attgcatcag
     1561 ttgctccttg ttcaaaggaa taggcatttc aagaagtgtt attccctgca aaccttcccc
     1621 aacaacgaga tcgccattca caaggaagag cggtcttgag ttcagctggc caagtttctc
     1681 aatttcatta tttagtctct cgtatccttc cttcgtggca tcggtctggg ttaggtccat
     1741 tagggattgg agtagttttc cgtaatatct aacattgctt tcgatgtagt actgatgatc
     1801 ctcgtatgct attcctaccg ttccccccgg cataaatggg ccggcaaaat ccagagattc
     1861 ggcggtaccg gaaacaacgg cggcaattac ggaggccgaa cccattgaat caaagagaaa
     1921 ttcctttcca cgcgcaaatg cccgtgccgc gtaaagccct gtgatatgat cgtggcctgc
     1981 gctaataaga ggaattcccg atttagtgtg accaactggt tcaccagtgt atctgagttc
     2041 gcccatcgtt tcttcgcttt cgccgatgta ctttatcagt tgcgagttcc atctgcgaga
     2101 gtgtatgttg accatgaagg atctgcatgc ctggctcata tcccaggcag gtacacctcc
     2161 cagcgagtaa gcaagatatg aagacactgg tagccagttc tccacacctt ctggcctgaa
     2221 gagttccttc tgaaggagta tcttgtagag cgagaaggtg taactatttt ccacccccgt
     2281 tattctgtaa ggagcgagat tgtcaaccga ttctctatga ctttcccata agggataagt
     2341 cgatctgtca taccacatca cgggttttga cagtgctttt ctctgcctaa cagggataac
     2401 cgtctctcca aagctggaaa aagctatggc agcaagagaa tgaagggtac cgacctttcc
     2461 gattatactc tcgacgtatg acctggtgag ttctagatct agaaactcga ctcctctctc
     2521 tttattcttc ggagtcgacg aatgtatcac ttcaaggatc ctcccatctt ctccaagaac
     2581 caggcacttg atgttcgtcg ttccaatgtc tatcccgaga aagacattca taataccact
     2641 tccgtaacaa ctacgtcgcc gattaccttt tctacatcag agtattcgaa aatcgcggct
     2701 ccatagtgca tggtattcgc ggcgccacaa gcggtcgcga tcttcagcgc ctctctcttc
     2761 gacatcttcg cctcttgacc atatatgagc ccggcaaaga aagagtctcc cgatccaact
     2821 gaaaagtcag aaaagacttt catcgatctc gcgtggagct tttcgctttc cgagagccac
     2881 acactgccca tttttccatt cgtgattatg agatccctta tcgaatattc ccttctgaaa
     2941 tcatccatcg acttgaaatc gtctttattg attccaaagg ctaccttcag ctcatccgcg
     3001 ttgattttta agagttccgg tgaaaccgtc acgatcttag acagccagga accggcaatg
     3061 tccactttaa gactgcaacc ggcctctctg gcaattctca caagttgaag aagtgagccg
     3121 ttctcgaacc ctctcggggc actcccggaa attacaaggt tcatacccgg tctaacatga
     3181 cctttgaaga actccataaa accctctatt tcgcccggct tcatgagagg tccaggttca
     3241 tttatcataa gcattttgtt ttcgtactcg taaacaaggg cagtgttaat tctgtttgag
     3301 tcttctatcc agaagttttc tgtcactatt cccagacgat cacattcttt ttcaattatc
     3361 gttccaacct gcccgccaag tatgtttatg cagaaatact cactgtgccc gagaaccttg
     3421 gatagtactc tcgcaatgtt gagtccctta ccgtccacga gtgttactac tttgtctccg
     3481 cgtaccacgt tcgggatctt tgtaggattc ttgagaataa cccagtgatc ataacaagga
     3541 tttaaattaa ggatacaagt atcgatgtta ttgtcctcct tcatcgtaaa ctatgtattc
     3601 tttcacaaac tttgctgtga actcatctgt tataagaaca tccgcaaagc cacctctaag
     3661 cattcccacc agaccatcca ctttgtggtg gccagcagct acaatgactc tttcgcctgc
     3721 attcatatat tgctcgaagc taaggttaat acacttgtcg aggatgctct tctcaaatct
     3781 tttgccgttt atatcgaagt aatgggtcaa tacgtctcct accacattgt tcttctcgag
     3841 ctcgtccgca tagctcactt cgagcaaatt ctctctgaat aggggtgatc gcgcgatgga
     3901 gtatccgaca ctacagatga taatatccag tgagttccag agtttctgga tgttcatgta
     3961 ctcaagggat tgctttgtat tctccagctg gcgggcgttt tcgaatatga agggcaaata
     4021 cattggaacg gcttttcccg atagccgagt ggcgaaattc tggacaatgt ggttgatgtt
     4081 aaaccttttg tcggcgagtc ttgcggtacc ccctgataaa ggtataagtt gccagtccgg
     4141 tctttcaagt ctctcaatcg attccgctac ggtaaagatt gtgttgcccc atccaagccc
     4201 gatattcata ggcttttcag gcaaagttct cagatattta tgaacctctc tcgacaaagc
     4261 cttcaagact gtcttttcgt tcctcgcgcc tctagcgatg aacatcttct tcagaccata
     4321 tctctccctg aagaaatcct cgtattccct tctaatggat gtctttaccg atggctgctc
     4381 gacttcaata tgaacgattc ctctttcttg cgccatcttg agatatttgc taatctgtac
     4441 tctggaaacg ccgtacttat tagcaatatc cttctggagc attcctttca cgtagtaatc
     4501 ggtcgcaacc tcgaaaagca gttcatcgct aatcatctct tactccttca cagatccgct
     4561 taccagtccc gctataaaga acttctgcaa gatcatgaat attatgagcg gcggaatcgt
     4621 cgccagtatc gatcctgcaa agagcggacc ccacctagtc tgatactgac ccacgaaaag
     4681 ctgaaggaac gtcgggattg tctgttttgc ctttgatgtc agaatagaca tcgagtacat
     4741 gagttcactc catgaaaaca agaaggcgta gacgcctacg gctgctatcc ctggaagcgt
     4801 gattggcaga ataacctttc tgaacgcctg gccaaaggta cagccgtcta ttcgtgcagc
     4861 ttcttccagc gatttcggta ttgataagaa gaaggttctc atgaacataa tagaaattgg
     4921 cgttgtgaat ggcaagtaag ataggatcaa gccaaaatat gtattgagaa ttccaatatt
     4981 gttgagcgtc ttaaaaatcg gtagaaggaa cactacgaca ggaagcatct gaaataccat
     5041 aactcctgta agaagaggcc tcttgaatct gaactcatat ctggcaaacg cataggcggg
     5101 aaacaatcca ataagtatgg agaagaacat tgtcccagtt cctacgatta cactgttctt
     5161 gaagtaagac cagaaattca tgtcggctat cttgtcaaag tgctctctcg ttggttcgaa
     5221 aatgaaatat tttattggat aggcatagat atccggatag gtttttatcg cagttaggat
     5281 aagccacaca aaggggaagg ctatcaccac tgataggaat attagcaaag aataggtaat
     5341 tgacttcttg acctttttct ttgtgcttct cttcatctcg cttcctcctt tgcaatgctt
     5401 ctcaggaaga caaatgcgat agcgaatatt gcaacgaaca ggatatttga aagtgcggct
     5461 gctttattga actccaggaa cctgaatcca agtttgtaga tataggtaac tacagtctct
     5521 gtaccgtatc ccggtccgcc tcccgtcatg atgtagatca gcgggaagta gttgatcgtc
     5581 cagatcacag taagcataag tgttgtcata atgaccggct tgagctgagg aatagttatc
     5641 ctccagaatc ttctgataga gttggcgccg tcgatttctg cagcttcata aagatcgggt
     5701 ggtattgtct gaagccctgc caggaaaaac attccagata ggcaaaagcc tttccatatg
     5761 tttgcaatta ttaccgagat catagccata ttttcagtag cgagccaggg tatgtaatca
     5821 tttattatgt tcattttgac cagaacatca ttgagcactc catacatatc gttgtacatc
     5881 cacttccaca tcacaccggc aacaatgtcc agcgttgccc agggaagtat gagaatgatg
     5941 ctcctgaata ttatctgccc ttttgtcgat tcatttagaa gcagagcgac gagcaagccg
     6001 agaagcaact gtccaagtac gcttcctatt acccagacaa cggtgttaat catcgatcta
     6061 ccgacaacag gatcggtgaa cacattggag tagttctgaa gtgtgaaggc accctcttcc
     6121 gttgtgaaac tgctgtagat attagagcct ataagataaa cgaagagcaa gactattatc
     6181 aaaagatagg gcagtaccat tttgaaggct gtcttctctt ctcttgcaag tttactcatc
     6241 tgttttcaac cctttcatca gggagtaatg aaagggcggg ctcttgcccg ccccatggaa
     6301 agtttatcac ttagccagta tagcgtttac atttgcctca gcagttttaa ttgcttcttc
     6361 aggtgttact tttccaagaa gaacgtcctg aatcatgatt tgaatctgct cagaaacctg
     6421 aggatattcg ggaattggag gtcttgagac tgcgtgtggg aaagtctcaa aaatgacctt
     6481 ccactttggg tcctgaagaa ctggtgaatc aaaacaaacg tcctgtctgg aaggcattgg
     6541 cccgccttta gcttcgatcc aggtcatcca ggtttcatag cttgtcaggt acttcgcgag
     6601 ttcccatgca ttatccttgt ttttacttgc agatgggata accagtgccc aaccaccgag
     6661 aacacttgcg ggtgttgctc cataaggatg aagagaaacg ctgtagttcc caactatatc
     6721 gggatttgct gtctcaatga gcgggaaaga ccaaggtccg ccaaccgcca ttgcaactct
     6781 gttctgagcc atcatgtttc tgtagtcatc ttcgttatac gtaactgcgt tggggcttac
     6841 ggcataagtc agcgctagat cggtatagaa cttgagagct ctgaggccct caggagtgtt
     6901 aaaggccgct gccgagtagt catccgtcaa aagctgacca ccagcgccgt aaaggaagat
     6961 tatgtaagcg agcgttgtgt gctcagtctt ggtaccgctg actccgtaac cgaacactct
     7021 tttgctggca tctgtaagct tcatagcctg atagaccagt tcttcccatg ttgccggtgg
     7081 atcgttcctg tcaagtccgg cctgatcgaa catcgtctga ttgtaaagaa gaagtcggca
     7141 atctgtatac cacggaagac cgtatctttc gccgttgtgc agaaccgtcg gccacagatt
     7201 cggccagatt tgctcagttc cgtcccagga gtcaagaaga gcgcccattg gttctaacca
     7261 gcccttctct gcaaatgttg gaacccatcc acctaggtcg gcgaacgcaa gatcagggcc
     7321 ctgacctgcc tgtagtgccg ttagaagttt gtcgtagtaa atgtcaaagc cgaacagttg
     7381 aacctctacc ttaagccccg tttcctcttc gaacttcccc acaatcattt cccaggtctc
     7441 atcgagctca gcaacttgtc cgccaatcca gatattgatt acctgggata ctcctgtgaa
     7501 agccagtaca accagcatca tgaataccag tagcgtagat tttcgcatgt aataacctcc
     7561 ctttttgtat taagttacat atgtaactta cttatgtaac catttaattc tataatcaac
     7621 aagctctaaa aaccaattcg aactagaatc gatgttgacc gattaaaacc attctaggtc
     7681 gaatagaagc aagaaactca tctctaataa gaattctctc tgaaaatgca ataagaaact
     7741 ctgatagaat cgctaaatta aaatgagcct taaaggtctg aactcaactg agagaaaaac
     7801 agaggattag ttgcaagaac aaaaagggga gcgcaagtcc agcgtcttgg ctctgggttt
     7861 ggtaaacaga agtcattgcc caccaatgat tatcagtgct actccagacg gaatttgaca
     7921 atttcgtaat tgcctaacat tgtcatcccg aacttgttt
//
LOCUS       DAOQYI010000066         7843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_9706_length_7843_cov_72.852594, whole genome shotgun sequence.
ACCESSION   DAOQYI010000066 DAOQYI010000000
VERSION     DAOQYI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9706_length_7843_cov_72.852594"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            485..1243
                     /locus_tag="PKL93_10085"
     CDS             485..1243
                     /locus_tag="PKL93_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNQ71647.1"
                     /translation="MIDRKEGRAFLGNKVWEEANSQLKKTDQMEGKPQPPLEKSSEGY
                     ELIKLPDPNVVNLHMKDLREIIFSRESRRIFSDDKISLESLSFLLWSTQGVKGVLRNN
                     YATLRTVPSAGARHPFETYLFVRLVKGLKKGLYRYVALSHSLILLKEGDFAKEVAEAA
                     FGQKFVGQCAVTFVWSVIPYRTEWRYDLTSYKPILLDAGHVCQNLYLACEALNLGTCA
                     IAAYDQTKTDELIGLDGYDEFAIYMAPVGKPKKY"
     gene            complement(1924..3306)
                     /locus_tag="PKL93_10090"
     CDS             complement(1924..3306)
                     /locus_tag="PKL93_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ71648.1"
                     /translation="MKKLLPIRIKLTLWYVLFLGVILFAFSILIYMILSAQLHSETDS
                     SLRIVSSQLLANIEIVQNYPVFKKTVEMDSLTNRLLAEGFAVRLMDNSGSIVEGFGPF
                     GILKASIDLTDKEFSSVSTSVGVWRIFTLQIGQWGWIQVGESLSSVRVALFRLSRLMM
                     LIGPTIIILAVVGGFYLAKKSLEPVERITLLAEEISVEKLNKRLNLTLPDDELGHLAS
                     TFDGMLERLEESVKKQRQFTSDAAHELRTPLTAIRSIVDVTLHRDRSSEEYVSALLQI
                     QNEIERLSSVIDDLLIITRLENTLPKREFAVFSVSALIWEVLETMMVLAKEKNIEIET
                     DLQENLEIAGDRKHLSRAVFNLVDNAIKYTPENGKIRIAAKLSESKERIEIIVEDNGI
                     GIQLEDMERIFDRFYRVDRARSQTRGTGLGLSIAKEIVKAHGGVISVKSSPGKGSTFK
                     VSLPSKIRKL"
     gene            complement(3311..3994)
                     /locus_tag="PKL93_10095"
     CDS             complement(3311..3994)
                     /locus_tag="PKL93_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ71649.1"
                     /translation="MSGGVVLRILIVEDEETISAFLQKGLREEGFVVDSAKDGNEAIR
                     LSSSNPYDVIILDVLLPIKNGFEVCSELRASGNHTPVLMLTALDSVDERVKGLNTGAD
                     DYLVKPFAFKELLARVNALLRRPRESFDRVLRVSDLSIDTVSHTVKRGDKEIELSPKE
                     YRLLEHLARNAHQVLSRTQIAESVWDQDFFSDSNVVDVYIRYLRKKIDDPFPTKLINT
                     VRGFGYKLG"
     gene            complement(4060..5748)
                     /locus_tag="PKL93_10100"
     CDS             complement(4060..5748)
                     /locus_tag="PKL93_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNQ71650.1"
                     /translation="MFRKGFVALIVILFVGFTAFSLTFEVSMSQEAIDTVASLGLETP
                     INGRVFVIVSKDSSSEPSMQTDVRGVPFWGKDVFKMTSESVVKISDGDMAVAGYPIDL
                     SDLPAGEYFVQALINVYTTFNRADGHTILMHDDTGDGQWFWESIGNATSKTKKVYLDP
                     ANPGSVELAITEVIMPDYDLEPGMVLQQGNYTDTEWVKYVKIKSDVVSEFWGKDMYIG
                     ANVLLPEGYYDNPDVYYPVIYYQGHFPGGGAPVGFRVDNDVYKFWTEDGNARFIAVSF
                     RDATPYYDTSYSVDSENSGPYGTAITEEFIPYLESQFRMIPEKWARVLAGGSTGGWEA
                     LAMKVFYPDFFGRTYVWYPDAVDFNYYQLINIYNDDNAYYSDFGWVKTERPSARDTHG
                     NIRFTVKQENYYEMAAGPSNRSGGQWSVWESTYSPVGVDGFPQPIWDQITGEINKDVA
                     EYWKENFDLNYILQENWEEIGPKIAGDVHVAVGDMDNYYLNEAVYLLKAAMEKMENPV
                     SDMTFDFGPNQGHGWKGWSPTDPNKALALQEWLLQLSDYMKENVPEGVETDWIY"
     gene            complement(6140..6376)
                     /locus_tag="PKL93_10105"
     CDS             complement(6140..6376)
                     /locus_tag="PKL93_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNQ71651.1"
                     /translation="MYYRSKENGIFNFNLYSNYKAVGSRYIATRPTEQEDVVEELNSE
                     DDAKLFEEFIWASLQRVRDYIDFKDFDSFCHGRR"
     gene            complement(7475..7651)
                     /locus_tag="PKL93_10110"
     CDS             complement(7475..7651)
                     /locus_tag="PKL93_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71652.1"
                     /translation="MTIKDYCEKAISRHSDNGPSQNLGLLGERTVLSSDQRIFVLKGI
                     AVLSREIPYRGTTE"
BASE COUNT         2090 a         1844 c         1723 g         2186 t
ORIGIN      
        1 cgaattctct gactttcatt tcttttgagc atcctgtcac aaaagtgtta gcatcgccgg
       61 gaaacgttgt aatcagtaat gatcagcgga gaccggttat ttaccctacg tagtccgaac
      121 tggcctcgcc gaagacgaga ctggccaccg aaggtgactg gcctgcgaag cagactggct
      181 acgcagatct tccccgcgaa gcgggcattg cgacctggca aagccagcct tgcatcccgc
      241 cgcaggcggc attgcgaccg ccgatgatat tcggcgcctt tcgtctttcg accaaatatg
      301 ctcttatagg aggacggtgg accgttgaca gttgattcga ggctcttctc gaaatgttct
      361 tgccagtttt gcgtccacga tttcttatca gaatcacgtt ttcgagattc ctctaatttc
      421 ttctgaatct gctattcttt gttatgctaa acttatttat atagctgagg aaggggaggg
      481 ttgcatgatt gatagaaagg aaggaagagc cttcttgggc aataaggtct gggaagaagc
      541 gaatagccag ctaaagaaaa cagatcaaat ggaaggaaaa cctcagcctc cactggaaaa
      601 aagctcggag ggatatgaac taatcaaact gcccgacccg aacgttgtta acctccatat
      661 gaaagacttg agggaaatca tcttttccag agaaagcagg agaatattct cagatgataa
      721 gatctctctt gagtcgcttt catttcttct ctggtccaca cagggagtca agggtgttct
      781 gagaaacaac tatgcgacac tgaggacggt cccctcagca ggcgctcgtc acccctttga
      841 aacttacctt ttcgtcagac ttgttaaggg tctcaagaaa ggtttatatc gctacgtagc
      901 tctgtcacat tcgctaatcc tcctaaagga aggagatttc gcgaaagaag tggcagaagc
      961 cgctttcggt cagaagtttg tcggccagtg tgcagtcacc tttgtttggt ccgtcattcc
     1021 ttacagaaca gagtggagat acgacttgac ttcatataag ccgattctcc tggatgcagg
     1081 tcacgtctgt caaaacctct acctggcgtg cgaggcactc aatttaggga catgtgctat
     1141 tgccgcttac gatcagacaa agacagacga gttaatcggt ttggatggtt atgacgaatt
     1201 cgcaatatac atggctccag taggaaaacc aaagaagtat tgattttgag tatctctcaa
     1261 ccagaaaagc tggttctaga aagtatatcc agaaaagtgt ttggaagaga ggagaggtat
     1321 tttccagtag aataagtagt tagggagtca gaccgttacc caggtgaact aaatgagatc
     1381 gtgcaagtcc tcggcgagaa gaactctatg atataaagcc cttcaaaatg catggaaata
     1441 cacgcaaagg tattctagaa gtagaagaag agaacaaacc cgaaaaggaa ggaattatgc
     1501 gggcattgtt gtcaaagata atggaattaa cttcggcaga ccgttctatt ttcatcaaca
     1561 ttgatgctct ggcaggaaag atgaacatga tgagatatga aacgttcgat gctctgaatt
     1621 tccttcacgg gcgatggtct tgtaaagatt cgctctagaa tctccgtgtc attaccagca
     1681 gaacagagaa agaagtcagt ctcctggctt ccatttgggg atgatgaaaa gggcattttt
     1741 cactttctca gaagcggagc ctgatttact atcgctttac cggagtagct taatcatcgt
     1801 caggctccct cttcgtaatt tgcattacct actctgcgta cagggatgga atctatgtga
     1861 atcttctatc gctgcatcaa cttaactata aggctcttac attagagaac aattagacta
     1921 tcattagagt tttctaatct tcgaaggtag gcttactttg aaggtagatc cttttcctgg
     1981 agagcttttt accgagataa cgccaccatg cgcttttaca atttccttag caatcgagag
     2041 gccgagacct gtgcctctcg tttgcgatct tgctctgtcc actctgtaga atctatcgaa
     2101 aatcctctcc atatcttcta actgtatgcc aatgccgtta tcttcaacga ttatctcgat
     2161 cctctcttta gattcactaa gttttgccgc aattcttatc ttgccgtttt caggcgtgta
     2221 ctttattgcg ttgtccacaa gattgaacac agctctcgac agatgctttc gatcacctgc
     2281 tatctctagg ttctcctgta agtcggtttc gatctcgata ttcttttcct tcgcgagaac
     2341 catcatcgtt tccagaacct cccagattag agcgctcaca gaaaagaccg caaattccct
     2401 tttcggaaga gtattctcca gccttgtaat aattagcaga tcgtctatta cgctgctcaa
     2461 cctctctatt tcgttctgaa tctgcaaaag agcacttaca tattcctcgg aactgcgatc
     2521 tctatgaagc gtaacatcca ctatgcttct gattgccgtt agcggtgtcc tgagctcgtg
     2581 agcggcgtca gatgtaaatt gtctctgttt cttcacagat tcctcgagcc tttcgagcat
     2641 tccgtcgaat gtactggcga gatgacccag ttcatcatcc gggagggtga gattcaacct
     2701 cttattgagc ttttcaacac tgatttcctc tgcgagaagc gtgattcttt caacgggttc
     2761 cagtgatttc ttcgccaaat agaagccccc aactactgca agaatgatta ttgtagggcc
     2821 aatcaacatc atgagccttg ataaacgaaa cagtgcaacc ctgacgcttg aaagagattc
     2881 gcccacttgt atccatcccc attgacctat ctgcagggtg aagattctcc aaactccaac
     2941 ggaggtcgaa acgcttgaaa actctttgtc agtcaaatct atcgaggctt tcagtattcc
     3001 aaacggtcca aaaccctcta ctatgcttcc agaattgtcc atgagtctta cggcaaaacc
     3061 ctctgccaga agcctgttgg ttagcgaatc catttcgacc gtctttttga atactggata
     3121 gttctgaaca atctctatgt tcgccaatag ctgtgaagag acaattctca gcgaagaatc
     3181 tgtttcgctg tgcagttgag cactcaaaat catgtagatg agaatgctga aagcaaataa
     3241 aatcacacca agaaagagaa cataccagag ggttaacttt atccgtatcg gaagtagttt
     3301 cttcattcta tcaacctagc ttgtaaccga atcctctcac agtattgatg agtttcgtag
     3361 ggaacgggtc gtctatcttc tttctcaaat acctgatgta gacatctact acattagaat
     3421 cactgaagaa gtcctgatcc cagacgcttt cagcaatctg agttctgctc aacacctgat
     3481 gtgcattcct cgccaaatgc tccaaaaggc gatactcttt tggcgaaagt tctatctcct
     3541 tgtcgccacg cttgacggta tgagaaacgg tatctataga cagatcgcta actctcagga
     3601 ccctgtcgaa actttctcgg ggtctcctga gcaacgcgtt cactctcgca agaagctcct
     3661 tgaaggcaaa gggtttcaca agatagtcgt ccgccccagt gtttagtccc tttactcttt
     3721 catctacgga gtcaagagcc gtgagcataa ggaccggagt gtggttgccg ctggctctaa
     3781 gttcgctgca aacttcaaac ccgtttttaa taggcaagag aacatccagt ataattacgt
     3841 catacggatt ggatgacgat agtcgtatcg cttcatttcc atcctttgcg gagtcaacta
     3901 cgaatccctc ttctctcaaa cccttttgga gaaaagcaga tatcgtctct tcatcttcga
     3961 caatcaagat tctcagaact acacctccag aaatcaaaaa accggagggg agaccctccg
     4021 gtcaattcta gcatcccaaa tcagtatctt ggatctatat cagtaaatcc agtcagtctc
     4081 cactccttca gggacatttt ccttcatgta atctgaaagc tgtaatagcc actcctgaag
     4141 agcaagggct ttattaggat cggtcgggct ccaacccttc caaccatggc cctggtttgg
     4201 accgaaatcg aaggtcatgt cggagactgg attttccatc ttctccatag ctgccttcaa
     4261 aagatagacg gcctcattga gataatagtt gtccatatcg cccactgcaa cgtgaacgtc
     4321 tccagcaatt ttcggaccaa tctcttccca attttcctga agaatatagt taagatcaaa
     4381 gttctctttc cagtattcgg caacatcctt gtttatttcg ccggtaattt ggtcccaaat
     4441 gggttgaggg aatccatcta ctcccacagg gctatatgtt gattcccata cggaccattg
     4501 cccgccggat ctgttgctcg gtcctgcagc catctcatag tagttctcct gcttaacggt
     4561 gaatctgata tttccgtgag tgtctcttgc actcggtctt tccgtcttta cccagccgaa
     4621 gtcagagtaa taagcattgt cgtcattgta gatattgatt agctggtaat agttgaaatc
     4681 aaccgcatcc ggataccaaa catatgttct cccgaagaaa tctggataga agaccttcat
     4741 tgcaagagct tcccatccac cggtagaacc gccagcaaga actcttgccc acttctcggg
     4801 aatcattctg aactggcttt ctaggtaggg aatgaattct tctgtaattg ccgttccgta
     4861 tgggcctgag ttttccgaat caactgaata agaggtgtcg taataaggtg tcgcatctct
     4921 gaaagaaaca gcgatgaatc tcgcattgcc gtcctctgtc cagaacttgt agacatcgtt
     4981 gtctactctg aatcctaccg gcgcgcctcc gccagggaag tggccctgat agtaaatgac
     5041 agggtaatat acatctgggt tatcgtaata gccttcagga agaagaacat tggcgccgat
     5101 atacatgtct tttccccaga attccgacac cacgtcactc ttgatcttta cgtatttcac
     5161 ccattctgtg tctgtgtaat ttccctgctg aagtaccatt cccggctcca gatcgtaatc
     5221 aggcattatt acttcagtga tagcaagttc tactgaccct gggtttgcag gatcaagata
     5281 gactttcttt gtcttgctcg tagcattacc aatggattcc cagaaccatt gaccgtcgcc
     5341 ggtatcgtca tgcataagaa ttgtgtgacc gtctgccctg ttaaatgtcg tgtagacatt
     5401 aatcagtgcc tgaacgaaat attctccagc aggcaagtca gatagatcga tgggatagcc
     5461 ggctactgcc atatctccat cggagatctt cacaaccgat tcagaggtca ttttgaatac
     5521 atctttcccc cagaagggaa ctcctctaac atctgtctgc atacttggct cactggatga
     5581 gtctttgctg acaataacga agacccttcc atttatcggt gtctcaaggc ctagagaagc
     5641 cactgtatca atggcttcct ggctcattga cacttcgaaa gtcagtgaaa acgccgtaaa
     5701 tccaacaaaa agaataacaa ttagggcaac aaaacccttt ctaaacatat ttcccacctc
     5761 catagaatcg tgagtatttg tggaattatt gtcaaaaatc gcaggtcaat aacggtcgta
     5821 ctcaaatgat tgccgagatt ggaattgagg gatgctagct ttgaaagact ttccttagaa
     5881 gcaacgacga cgagcgcgga gagagcgaag tgagtcttca cacgaatgaa gtttcacact
     5941 ttgaggttgg ttaagtttca taaattcgca ttcaacaaca gaaagaaaga tgaactccat
     6001 tgtttacctc ctcttccaaa ccctattagg tcggtctttg ctattataca ctcctgaagg
     6061 ttatgaaagt acactatgcg aatttattta catcatctta tgttttccgt tgctttgctc
     6121 gttctttctt caaacctcgc tatcttctac cgtgacagaa actatcgaaa tccttgaagt
     6181 cgatataatc tcgaactctt tgcagagaag cccatataaa ctcctcaaag agctttgcat
     6241 catcttcgct gtttagttct tcaacaacat cctcttgctc cgttggacgt gtagcgatgt
     6301 accgactccc tactgccttg taatttgaat agaggttgaa attgaaaatc ccattttcct
     6361 ttgatctgta atacacagct tacacctcct gcatttcata acaattatat catcctctgt
     6421 aacaagcgta attgggggct tcaggcaggg gggaggaaag agcaagggat cagatgcaag
     6481 ttgtgagttg tgaattgtcg taaaaaaggc cggggttgga ggttggtggt tggaggtccg
     6541 taagagcaag agaaactgat tgaccgttga gcggttctac gttcgccggg gagagccgtg
     6601 aagcaatagc atcgagattc cgaccagtag ctttgtcgga atgacggaat gaggcgtttt
     6661 cgggaaaacc atctgctgtc atcccgaact tgtttcggga tctcggtttt cgaatataac
     6721 gtggtttgcg atagcacgta gggcgttgga aagagccaac tgaaaccggt tcgccgttga
     6781 gcggttctcc gttctccgag aagagccgat caccgcttgc taggagacgc ttaacgctgg
     6841 aaagagcaaa agatcagttg taacttgtga gttgtaagcc cgtagttggg ggctcgaaag
     6901 agcagattga aaccggttcg ccgttgagcg gttctacgtt cgccggggag agccgtgaag
     6961 caatagcatc gagattttga ccgggagatt tgtcggaatg acggaatgag gcgttttcgg
     7021 aatgaccaag cgttgtcgtc acgatggtag ttttgggcga cttttcgcat cgttatcacg
     7081 aaacggtttt cggtataacc attcgctgtc atcccgaact tgtttcggga tctggttttt
     7141 atcctctcaa cggaggacgg tgtactgttg actggctaac gtttttcata agcgttcagc
     7201 gggttcttgc tcctaagcgg acagcggctt ttagacagaa attctgacca ggggcattgt
     7261 cagaatgacg ggatatggcc ttttcgcaat aatctttaat tgtcattccg tagtgctcct
     7321 gtacgggatc tcgcaactaa gaacagtcat cccctaagaa cgaagacccg ctgatcgctg
     7381 gaaagaacag tcctttcacc caaaagaccg agattctgac taggaccatt gtcagaatga
     7441 cgggatatgg ccttttcgca ataatcttta attgtcattc cgtagtgccc ctgtacggga
     7501 tctcgcgact aagaacagct atccccttaa gaacgaagat ccgctgatcg ctggaaagaa
     7561 cagtcctttc acccaaaaga ccgagattct gactaggacc attgtcagaa tgacgggata
     7621 tggccttttc gcaataatct ttaattgtca ttccgtagtg ctcctgtacg ggatctcgca
     7681 actaagaaca gtcatcccct aagaacgaag acccgctgat cgctgggaag aacagtcctt
     7741 tcacccaaaa gaccgagatt ctgactagga gcattgtcag aatgacgaca tgtgttgctt
     7801 tcagaataac cgaagagcca tttaacatag tcattctttc tcg
//
LOCUS       DAOQYI010000067         7801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_9758_length_7801_cov_83.556029, whole genome shotgun sequence.
ACCESSION   DAOQYI010000067 DAOQYI010000000
VERSION     DAOQYI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9758_length_7801_cov_83.556029"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKL93_10115"
     CDS             complement(<1..217)
                     /locus_tag="PKL93_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNQ71653.1"
                     /translation="MEKRLPILEHDNERPAVIEPTMVISSVSEMPERAIILFYQDVIE
                     TLLNKGLLKKIIDRRSEVGLYPVYEIEY"
     gene            336..1157
                     /locus_tag="PKL93_10120"
     CDS             336..1157
                     /locus_tag="PKL93_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71654.1"
                     /translation="MKTRFVLIVVLLIAVVSSAGMLNKPENRFKLELDYEIGFTGVIF
                     HTIQFGQTGTVLNYVKDGGQDVLFPYQRYTAGLSIGKHTVYFLYQPLTVQSKVQLKND
                     LIVDGVTFPAGSGILLNYGFPFYRISYTYDVLRKGNSYLALGLSLQLRNADIYFESLD
                     GTKFVDNKNVGPVPIIKVKGAYWLNEKVYLATDIDGFYASSSFINGADFDFEGSVLDA
                     SLRAGLLLTDYLETYFNVRFIGGTAKGTSENSDSPDGFTDNKLATINFSIGFQLK"
     gene            complement(1232..1888)
                     /locus_tag="PKL93_10125"
     CDS             complement(1232..1888)
                     /locus_tag="PKL93_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNQ71655.1"
                     /translation="MCRMIAFKADKNIDTNWILDILKGISEKGISSPHSDGWGYAVCG
                     EGRETYNSSGAIFDSDKRLPQSRVGIIHSRKASKGYVVNLEHVHPFYATVRGRNYAFC
                     HNGTIYDFASDEGTTDTQRYLELLVSNLEIYETKRALEITAAFVAENYRYTSINALLT
                     DYENVWAIRQNAETNDTEHALFLYERDGVRIVSSEPVEKYGDLPGNSVEIKNGESIVL
                     "
     gene            complement(1876..2478)
                     /gene="pdxT"
                     /locus_tag="PKL93_10130"
     CDS             complement(1876..2478)
                     /gene="pdxT"
                     /locus_tag="PKL93_10130"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNQ71656.1"
                     /translation="MKIGVLGIQGAIQEHLSILRKAGVEPSWVKDRNELDSVEALVMP
                     GGESTTMIKLLKRFEMWEALRNRIEDGMPVLATCAGMILLSKKIENVVNQDSLGVLDI
                     SVKRNGYGRQINSFEVDLQIDEIGPEPFRAVFIRAPKIESIGDEVRVLTSYDGSPVLV
                     RQNNMLAASFHPELTGDLRIHRYFLKMAEAAGQRIEKCAE"
     gene            complement(2482..3360)
                     /gene="pdxS"
                     /locus_tag="PKL93_10135"
     CDS             complement(2482..3360)
                     /gene="pdxS"
                     /locus_tag="PKL93_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNQ71657.1"
                     /translation="MEARGTWTVKKGFAEMFKNGVIMDVTNPEQAKIAQDAGATAVMA
                     LERVPADIRKEGGVARMAKIGLIKEIMETVTIPVMAKARIGHIAEAKILEAIGVDFID
                     ESEVLTPADDKYHIDKRIFTVPFVCGARNLGEAVRRIAEGAAMIRTKGEAGTGNIIEA
                     VKHMRTVNEEVRKVQMMNDAELVHYGKEIGAPVEILSQVRELGRLPVVNFAAGGVATP
                     ADAALMMMLGCDGVFVGSGIFKSKDPARMAKAIVEAVLHYDNPEALAEISENVGDAMD
                     GLEIETLEVRMEERGW"
     gene            complement(3363..4679)
                     /locus_tag="PKL93_10140"
     CDS             complement(3363..4679)
                     /locus_tag="PKL93_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNQ71658.1"
                     /translation="MDKRRIDPRVFKVPIDKMRAGYYSDKYFTRLVEVLKKADKKSRV
                     LYQLFPRRDATIVGIDEALAILRFGTGYYSNEEEAKRLFEEVLEIEKAVNHAAWEMDR
                     HALVELTAQKWDLKMMLNDLWVDKWPELEVRALYDGDEAKDMEPVMTIEGDPRYFSYL
                     ETILLGVIARASSTATAVKSVVKAARGKEILFFSARFDHFWTQATDGYAALKAGAFGV
                     STDANADYWGVESMGTIPHALIAVYNGDTAEAALAFDRYIEGGVNRIILVDWDNDVIG
                     TTVDCVARTYKAETGENFVPGKTDPSVVIGPGKGKIWGVRFDTSGSLRDVSVVPKDAS
                     SLGVCPELVWRARREFDRYGMKDLKIVVSGGFTAEKIDLFEKLNVPVDVYGVGSSLLK
                     NKLDFTADIVEVEGRPCAKVGRGKIETPRLEKVASDYWNNDKEERC"
     gene            complement(4856..5821)
                     /locus_tag="PKL93_10145"
     CDS             complement(4856..5821)
                     /locus_tag="PKL93_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71659.1"
                     /translation="MKFGALFAIFLGMLIILSVTHVIPSFSFAIEIFLAIIFLYQGLK
                     VFKRFKPEHMGSLIFGGILFTDLLIGWNVIKGLRGWGFWELVVAMIGSYIVGWGIVTL
                     FKRSFKLGETPNSTRQVLNVSRPKEFEELEIDIDANLTKVLLMDNTSNGFDANVSYDK
                     QSFSGDLKYDMDKGKGSLRARCKARSGVSSVLSKSRMNFELNSEPILRLDATLDGADS
                     VFDFSKLNLDSARIKTSLSRLSIIPSQLRDSIIDIDCEVTSLNIRTPKDVGLSIIHEG
                     ELNWSNFNNLMEREKGYVSTNIDRAVTTCQIIVKSDMSKISIDWI"
     gene            complement(5846..6940)
                     /locus_tag="PKL93_10150"
     CDS             complement(5846..6940)
                     /locus_tag="PKL93_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNQ71660.1"
                     /translation="MEKKFEFDSAGIEILKISSASADLQIDLVEDGNVIAYVEVDSND
                     YVPEAESSGKRLSIKFQKGTNISIPFIKNLFDGGAEVKSVRLLVPKSVRELDASSASG
                     DIFITDFDLRSLKISNVSGDVKIENCSVENLNFNTVSGDLNFIASNYRRGKFGSVSGD
                     LRIKGLPPMERETTVSSVSGDAIVSYSGEPKFDATISSVSGDISSDVGLVKVDKKHYR
                     SGNGESQEYLKMNSVSGDLIISTKYTGTPAKEVKVESRTVSSEEKKGHRSAVVEENLQ
                     DQETRKILLLFKEGKLTKEYTFEMLGILGYSEQEIEEMLEVDEELQKFKDSVDVVIKN
                     EDVESEKSFEALEKPETPEKPDTPEEPKSI"
     gene            complement(6953..7315)
                     /locus_tag="PKL93_10155"
     CDS             complement(6953..7315)
                     /locus_tag="PKL93_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71661.1"
                     /translation="MDREEVLKILKLVADGKVSPEQGRDLLEEFFESYEEVKLGRKFS
                     IEVVDNHSGKVEAHIRLPVRLAKFVGNFVKEKDANFKIGDRVVDYNLKEVLDEVIKSG
                     ESVEIETGDKRVTIKIED"
     gene            complement(7325..7699)
                     /locus_tag="PKL93_10160"
     CDS             complement(7325..7699)
                     /locus_tag="PKL93_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNQ71662.1"
                     /translation="MKYSISNCPVCGGKMTITEYKCDSCGTTIRGRFELDDIMKLSAE
                     QLDYLKTFIKNRGNLSEVQKELDISYPTARNRLEDIVRAMGYQVVDKDREEASRILER
                     LESGELQPDEALEMLRKVRKKK"
BASE COUNT         1803 a         2139 c         1618 g         2241 t
ORIGIN      
        1 tgtactcgat ctcataaacg ggatacagtc cgacctcgct tctcctgtca atgatcttct
       61 tcagaagacc cttattcagg agagtctcaa taacgtcctg ataaaagagg attatcgctc
      121 gttccggcat ctcggagacc gaagagatga ccatcgtcgg ttctattacg gccggtcttt
      181 cattatcatg ttccagaata ggtaaacgct tctccatata tgcccccttt acgacaattt
      241 tagcagtgaa gaagagatcg acagtcgccg aatgacctga cacttttgta accggaagtt
      301 ctgttattgt aatcagtata aacggaggtg gaatcgtgaa gacaaggttc gtactgatag
      361 tggttcttct tatcgcagtt gtttcatcgg ctgggatgtt gaacaaaccc gaaaacaggt
      421 tcaaactcga gcttgattat gagatcggat ttacaggagt gatcttccac accattcagt
      481 tcggtcagac gggaaccgtt cttaactacg tgaaagatgg cggtcaggac gttctctttc
      541 catatcaaag gtacaccgcc gggctgagca tcggcaaaca tacggtctac tttctgtatc
      601 agccgctcac cgttcagtca aaggttcagc ttaaaaacga cctgatagtc gatggagtca
      661 ccttccccgc cggaagcgga atcctcttga actacggctt ccccttctac aggatcagtt
      721 acacttacga cgtcttgaga aagggcaaca gctatcttgc cctcggtctc tcacttcagt
      781 tgagaaatgc agatatctat ttcgagtctc tcgatggaac gaagttcgtc gacaataaaa
      841 atgtcggtcc cgttccgatt atcaaagtga agggcgcgta ctggctgaat gaaaaggttt
      901 acctagcaac agacatcgac ggcttctacg cctccagcag tttcattaac ggagccgact
      961 tcgactttga gggatctgtg ctggacgcct ctctaagagc cggtctgctt ctaacggatt
     1021 atctcgaaac ctatttcaat gtacgtttca taggtggaac cgctaagggc acttctgaaa
     1081 actccgacag ccccgacggc ttcaccgaca acaaacttgc gaccattaac ttcagcatag
     1141 gcttccagtt gaagtagaat agctacaagt atataagaac cggccataat ggccggttct
     1201 tttctttaat ccgcaatttt attaggtctt actaaaggac tatcgattcg ccgtttttta
     1261 tttcgacaga gtttccgggg agatcgccgt atttctcgac aggttcgctg gagactattc
     1321 ttaccccgtc tctctcgtaa agaaacaggg cgtgttccgt gtcgttcgtc tctgcgtttt
     1381 gccttatcgc ccagacgttt tcatagtccg tgagaagcgc gtttatcgac gtgtagcgat
     1441 agttctccgc cacaaaggcc gcagttattt ccagagctct cttcgtttca tatatctcaa
     1501 gattgctgac cagaagctcg agatatctct gcgtatcagt cgttccttca tcgctagcga
     1561 agtcgtatat cgttccgttg tggcagaagg cgtagttcct tcccctgact gtggcgtaga
     1621 agggatgaac gtgttcaaga ttgacaacgt atccctttga ggcctttcga gaatgaataa
     1681 tgcctaccct gctctgagga agcctcttgt cgctatcaaa gatcgcgccg gaactgttgt
     1741 aggtctctct tccttcgccg cacacggcat agccccaacc gtcgctgtga ggactggaaa
     1801 tgcctttctc ggagatgccc tttagaatgt ccaagatcca gttagtatct atgttcttat
     1861 ctgccttgaa ggcaatcatt ctgcacattt ttcaatcctc tgtccagccg cttcggccat
     1921 cttcagaaag taccggtgaa tcctcagatc tccggtgagt tcgggatgaa aggaggctgc
     1981 cagcatgtta ttctggcgaa cgagaaccgg tgatccgtcg tagcttgtaa ggactcttac
     2041 ttcgtcaccg atcgattcga tctttggagc tcttatgaat actgccctga atggttcggg
     2101 tccgatctcg tcgatctgta gatcgacctc gaagctgtta atctgcctgc cgtaaccgtt
     2161 tctcttgacg ctaatgtcta gaacgcccag agagtcctga ttgacgacgt tttcaatctt
     2221 cttggacagg aggatcatac ccgcgcaggt tgccaggacc ggcattccat cttctatcct
     2281 attcctgagg gcctcccaca tttcgaatct cttcaagagc ttgatcatag tggtcgactc
     2341 tccacccggc atcaccagcg cctcgacgga gtcgagctca ttccgatcct tcacccagga
     2401 gggttccact cctgccttcc tgagaatcga tagatgttcc tgtatagcgc cttgaattcc
     2461 gagaaccccg atcttcaacc cttaccagcc tctttcttcc attcgaactt caagagtctc
     2521 gatctcgagt ccgtccattg catctccgac gttctccgag atctctgcca gcgcttcggg
     2581 attgtcataa tgcaaaactg cttcgacgat cgctttggcc attcttgcgg gatccttcga
     2641 cttgaagatt cccgaaccta cgaagactcc gtcacagccg agcatcatca tgagagctgc
     2701 gtcggccggt gtagccactc cgccggcggc gaaattgacg actggaagtc ttccgagctc
     2761 tcttacctgg ctgagaatct cgacaggcgc accgatttcc tttccgtagt ggacgagttc
     2821 ggcgtcgttc atcatctgga cctttctcac ttcttcgtta acggttctca tatgcttgac
     2881 tgcctcgatg atatttccgg tgccagcctc tccctttgtt ctaatcatag cggcgccctc
     2941 ggcgattctc ctgactgctt ctccgagatt tctcgctcca caaacgaagg ggacggtgaa
     3001 tattctcttg tctatgtgat acttgtcatc tgcaggtgta agaacctccg attcgtcgat
     3061 gaagtcaacg ccaatagcct caaggatctt cgcttcggcg atgtgaccga ttctagcttt
     3121 ggccattaca gggatcgtta cagtttccat gatctctttg atcagaccaa tctttgccat
     3181 tcgggctact ccgccctctt ttcgaatgtc cgccggtact ctttcaagag ccattactgc
     3241 cgtggctccg gcatcctgag ctatctttgc ctgctcggga ttcgtaacat ccatgattac
     3301 gccgttcttg aacatttcag cgaacccttt ttttactgtc caggttcccc ttgcttccat
     3361 tttcaacacc tctcctcttt atcgttgttc caatagtcac tcgctacctt ttccagtctt
     3421 ggagtctcta tcttccctct tcccactttt gcgcagggtc ttccttcgac ctccacgata
     3481 tctgccgtga agtccaactt gtttttcagc agggacgaac cgactccgta aacatccacg
     3541 ggtacattca gcttttcgaa aagatcaatc ttctccgccg tgaaaccacc cgagacgact
     3601 atcttcaaat ccttcattcc gtacctgtcg aattcccttc tagccctcca gaccagttcg
     3661 ggacagactc ccagcgaaga agcgtcctta gggacgaccg aaacgtctct caaactgcca
     3721 gaagtatcga atctcacgcc ccatatcttt ccctttcccg ggcctatgac aaccgaaggg
     3781 tcggtcttgc cgggtacgaa gttttcaccg gtttcggcct tatacgtgcg tgcaacgcaa
     3841 tcgaccgtcg ttccgatcac atcattgtcc caatcgacca aaattatccg gttgactccg
     3901 ccctctatat aacgatcgaa agccaatgcg gcctcggccg tatctccgtt atacactgcg
     3961 ataagagcgt gaggaatcgt ccccatgctc tcaacgcccc agtagtcggc attcgcgtcg
     4021 gtagagactc caaaagcccc ggccttgagc gccgcgtatc cgtccgttgc ctgagtccag
     4081 aagtggtcga atctcgcgct gaagaagagt atctccttgc ctctggcggc cttcacgacg
     4141 gacttgacgg cagtcgcagt tgaggatgct ctcgctataa cacccagcaa tatggtctcg
     4201 agatacgaga aatatctggg gtcgccttct atcgtcataa ccggttccat atcctttgct
     4261 tcatcgccgt catacagcgc gcgaacttcg agttcgggcc atttgtcgac ccagagatca
     4321 ttcagcatca tcttcaggtc ccatttttga gcggtcaatt ccaccagggc gtgtcgatcc
     4381 atctcccatg ctgcgtggtt cactgccttc tcaatctcga gcacctcttc gaagagcctc
     4441 ttcgcctctt cttcattcga gtaataaccg gtcccaaaac gaagaatggc gagcgcttcg
     4501 tcaattccca ctattgtcgc gtctcttctc ggaaagagct ggtatagcac ccttgacttc
     4561 ttgtctgcct tcttgagaac ttcgacaagt ctcgtgaagt atttgtcaga atagtatccg
     4621 gctctcatct tgtcaatcgg tactttgaag accctcgggt cgattctgcg cttatccacc
     4681 gaacgccccc tgtctatgcc ctcaatcgcg agcaaaaatt ccggaaaaaa cacatccggt
     4741 accctgatta tataataata aacgactttc gactcttgaa ccgggcagat ctttgccaat
     4801 tggaccagtc caaaaaaata aaccggccat aaggccggca gagaagatga cgcttctata
     4861 tccagtcgat agagatcttc gacatatctg acttcacgat gatctggcaa gtcgtaacgg
     4921 ccctgtcgat attggtcgag acgtatccct tttccctctc cataaggttg ttgaagttgc
     4981 tccagttgag ctcgccttcg tggataatgc taagaccaac gtctttgggt gtcctaatgt
     5041 tcaaggaggt tacctcacaa tctatgtcta ttatagagtc tctcagctgg gaaggtatta
     5101 tagaaagcct ggagagcgag gtctttattc tcgcgctgtc aagatttagt ttggagaagt
     5161 cgaagacact gtcggcgcca tcaagtgtag catccagcct gagaatcggt tccgagttga
     5221 gttcgaaatt cattcttgat tttgacagga cactggatac tcctgacctt gccttgcacc
     5281 tggcccttag agaacccttt cccttgtcca tatcgtactt tagatcgcca ctgaaactct
     5341 gcttatcgta actcacattt gcatcgaagc cgtttgaggt gttatccata agcaatacct
     5401 ttgtgaggtt ggcatcgatg tcgatttcca attcttcgaa ctctttgggc cttgaaacgt
     5461 tgagaacctg tctcgtagag ttcggtgttt ctccaagttt gaagcttctt ttgaaaagag
     5521 taacgatacc ccagccaacg atatacgatc caatcatggc cactacgagc tcccaaaagc
     5581 cccaacccct gaggcccttt ataacattcc agcctattag tagatctgtg aagaggattc
     5641 ctccaaaaat cagacttccc atgtgctctg gcttgaacct tttgaagacc tttaatccct
     5701 gatacaggaa gataatcgca aggaatatct caattgcaaa ggaaaagctg gggatcacat
     5761 gcgtgacgga aaggataatc agcattccaa gaaaaatcgc aaatagtgct ccgaatttca
     5821 ttttactatc accttctttt ccctatcaga tcgatttagg ttcttcggga gtatccggtt
     5881 tttcgggtgt ttctggcttt tctaaagctt caaaagactt ttcactttca acatcttcgt
     5941 ttttgattac tacatctacc gagtccttaa acttctgaag ctcctcgtcc acttcaagca
     6001 tctcttcaat ctcctgctca gagtatccga ggatgccgag catctcaaag gtgtactcct
     6061 ttgtaagttt gccttccttg aagagaagaa gaatctttct cgtctcctga tcctgaagat
     6121 tctcctcgac aacggccgat ctgtggccct tcttctcttc cgaagagacg gttctggact
     6181 caacctttac ttccttcgct ggagtccccg tgtacttcgt actaatgatg agatctcccg
     6241 aaacactgtt cattttcaaa tactcctgcg attctccgtt tcccgatcta tagtgtttct
     6301 tatctacctt cacaagcccg acgtcagatg agatgtcacc gctcacagag gagattgtgg
     6361 catcgaactt aggctctccc gaataggaga caatggcgtc tccactgacc gaagaaactg
     6421 tcgtttccct ttccatagga ggaagcccct taatcctgag atcacccgat accgagccga
     6481 atttacctct tctataattc gaggctatga agttcaagtc tccgcttacc gtattgaagt
     6541 tcaggttttc tacagagcag ttctcgatct tcacgtcacc gctgacgttg cttatcttaa
     6601 ggcttctaag atcgaagtcg gttatgaaga tatcaccgga agcactgctg gcgtctaact
     6661 cccttacaga cttaggcacg aggagtctta cgctcttgac ctctgctccg ccgtcaaaga
     6721 gattctttat gaagggaatg cttatattcg ttcccttctg gaactttata ctcagcctct
     6781 tccctgaact ctcagcttcg ggcacataat cattagaatc tacttcgacg tatgcaatca
     6841 catttccatc ttccacaagg tcgatctgaa gatccgcgct agctgaagag atcttaagaa
     6901 tctcgattcc ggcgctgtca aattcaaact ttttctccac gaccataccc cctcaatcct
     6961 ctatctttat cgttaccctc ttgtccccgg tttctatttc aacggattcg ccgctcttga
     7021 ttacctcgtc caggacttcc ttcagattgt agtcgacaac cctgtctcct atcttgaagt
     7081 tcgcgtcctt ttcctttaca aagtttccga cgaattttgc tagcctcacg ggaagtctta
     7141 tatgggcctc gaccttcccc gaatggttat ccacaacctc tatagagaat ttccttccca
     7201 actttacttc ttcatatgat tcaaagaact cttcaagcaa gtcccttcct tgctcgggag
     7261 aaaccttacc atctgcaacg agcttcagga tttttagaac ctcttcccta tccaattaga
     7321 tcacctactt cttttttctg actttcctga gcatctcgag agcttcatct ggctgcagtt
     7381 ctccggattc aagcctctct aggattctcg acgcctcttc cctgtccttg tcgacaactt
     7441 gatagcccat tgccctcact atatcttcga gtctgtttct cgccgtcgga taggagatgt
     7501 ccaactcctt ctgaacctcg gagagattgc ctcgattctt gataaaggtc ttcagataat
     7561 caagctgttc ggcggagagt ttcataatat cgtcaagctc gaatcttccc cttatcgtgg
     7621 taccgcaact atcacatttg tactcagtga tcgtcatttt ccccccgcag acgggacagt
     7681 tgcttataga gtatttcatc ggtccacctc cacaacttaa gttgaaccta aagatacttt
     7741 caaccacatt cattatacaa cttctaactt gaaaagtcaa acattacctt aaatatattt
     7801 a
//
LOCUS       DAOQYI010000068         7630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_10014_length_7630_cov_77.595908, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000068 DAOQYI010000000
VERSION     DAOQYI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10014_length_7630_cov_77.595908"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1176)
                     /locus_tag="PKL93_10165"
     CDS             complement(4..1176)
                     /locus_tag="PKL93_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNQ71663.1"
                     /translation="MDRISNFEYFLPTRLVFGVGSLNRVGEFAKSLGKKALIVTGKSS
                     TKKTGLLDRVIAVLEKNGVETAVFDEIVPNPLSSTVDKGAEFANNEGCDLIIGLGGGS
                     PIDSAKLIAVVAKDGGKCWDYTGSGGGRIPKTALPVIAIPTTHGTGTESDPFAVVTNT
                     ETDEKIGVGFDQTFPTVSIVDPEVMKTLPPYQTAATGMDAFYHAIESYINTNHQPTSD
                     LLALEAMSLINHYLPIAYRDGNDIEARTALAWASTAAGICETLSGCIANHSIEHPISA
                     HYNATHGAGLCATGPAFFDYIRPHTKERLARVAQIMGAPESEIDIDKLSKMSIELIHR
                     LQKSVDIDITLKDLGVEKSMLGRLAEDAMRTMGALVEVTPGNLKTKDLERILEMSY"
     gene            complement(1243..2043)
                     /locus_tag="PKL93_10170"
     CDS             complement(1243..2043)
                     /locus_tag="PKL93_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ71664.1"
                     /translation="MKITVLCNDKGLEGFKSEHGISFFIELNGKKFLFDTGSTDVAVK
                     NAEKLGIDLSEVESIIISHGHYDHLGGLGSVLKKTGAKKVMVGEGCLERKFSGSTESS
                     PGGCGSEYEQLGASIETVRASREVTNGLHVMTAAPFVTEERPGDKHNRIKGGTRIRDF
                     FEDELSLAVVQNGVVTVITGCSHRGIGNIVKQASGFGKVKNVVGGLHLLHKTEDELEE
                     IFEYLLGFDIDKFHIGHCTGDNVVKKIAERFPAEVRELMAGDSFEFHG"
     gene            complement(2191..3018)
                     /locus_tag="PKL93_10175"
     CDS             complement(2191..3018)
                     /locus_tag="PKL93_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNQ71665.1"
                     /translation="MIQDFISDIISYSFLRNAILAAVLVSALTGIFSSVVVLRKIEFI
                     GDGAAHATFGGIAFGLLLGTNYLLMAAVTAVIFAVAVSYFTRKNKLSESSVIGMLLPL
                     SMSLGVIALSFIRGYTPDVMGLFFGNILMVTSEDVWMLLIANVIAWVFFAVFYRELLY
                     YSYDEGMAKHYGVPIGFIHYGVLIGISLSVVGSVKIAGIILVTAFLVIPAVTSKTIAG
                     SFRGMILISLAMAVVASVMGIFVSYVLDMPPGPVIVVMLFMEFLITISLKALIRVRF"
     gene            complement(3015..3755)
                     /locus_tag="PKL93_10180"
     CDS             complement(3015..3755)
                     /locus_tag="PKL93_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNQ71666.1"
                     /translation="MENKILSVENLSYRIGSHWILRDVSFSISRKEFVGIIGPNGAGK
                     TTLIKAIVGDLEGYSGKIVVSGKIGYLSQNPDRKRDFPIKVREVAAMGLYGDRGILRP
                     FRKSDWIRVDDLLRTVGILDLRDRKAGTLSGGEYQRLMLARALASDPDLLILDEPEAG
                     VDEMGKASFYRLLESLKNEKSIAILMVSHDIGMVFDACDTVMCINKTLHCYNDADNIT
                     PEELQSAFSSDLDFFIRSRDHFEREHHL"
     gene            complement(3745..4629)
                     /locus_tag="PKL93_10185"
     CDS             complement(3745..4629)
                     /locus_tag="PKL93_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNQ71667.1"
                     /translation="MKKRTLLTITALMILFSTTLLPLKIVATINPYYLIVREIVGQEG
                     QVDLLIGPGQNPHIFSPKISDVRKLNEADLVIANGLDLESFLESYLEDLAAQGKHVLY
                     MGNQLPDDFLVSEDEIGEANTDLHHFNPHIWLDPVILSDYLIPVIVEKLSNIDPANSV
                     LYKVNAEKLISELHDFDEKASSYLERFRGSIVIVAHPSFTYFFRRYGIELEPVLEGVG
                     DEPSIGEIKELVDFVREKDVIGIFAEYQHSKRAIDILKSETAVGHGELDSLGLSMGSI
                     IELLQWNLDEMMRVFDGK"
     gene            complement(4626..5030)
                     /locus_tag="PKL93_10190"
     CDS             complement(4626..5030)
                     /locus_tag="PKL93_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNQ71668.1"
                     /translation="MKRLTSLRREVLDLINNSESPLSADSIYGKLKEKPNLSSVYRSL
                     SFLEDEGLIRSISFECETRFYFSSEREPVHFLHCKKCHKTEPFYEGFADSFAKKVAES
                     RDFTITDHVFYFIGICGECKRKMLSDIEDGKL"
     gene            complement(5119..5967)
                     /locus_tag="PKL93_10195"
     CDS             complement(5119..5967)
                     /locus_tag="PKL93_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNQ71669.1"
                     /translation="MKRSVFIVLSLVFFFSFFLLQGCDRSKLTTAVTSVIDGDSITVK
                     ALQGTVRLIGIDAPEVTSGSKPAGQFAEEAREFLKQMVLSNGKVTLELHGKDSYGRHL
                     AYLFNSEGEFVNAELVRQGLARPLTYEDTSHNSAKIREAYRQAFNNRSGIFSSYNDFQ
                     VYDASEVREALYPYQLGGFLGKIVWVEFTVIDVNGFTVVGDDIVVRVRPEEATLFGQD
                     SEDLQRLYRKKIRVFGEIWQDDSGKALILLRDPAIEITGISQFANSSPFFFQKLLEIL
                     PMRKQS"
     gene            6133..6921
                     /locus_tag="PKL93_10200"
     CDS             6133..6921
                     /locus_tag="PKL93_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ71670.1"
                     /translation="MKDNISSEQLSELLTQALALIEDGVAVLDEFSNVLVANSRASIL
                     IEEVQEEVKKKIRDLTRRSEEALEYHHIRKNGRWLRYYLKKLSVPNALYFLFVVEDIS
                     DLKLAEQTISELAHFDGETGLPNYVLFRDRLNMAIFRSRRNNLFSMVAAFELSNREAV
                     QSIQEATLVEITEELVKGIRKSDTLCRFSRREFLLLAEDLKNPDSAARIIGKVLSSFE
                     KWKLDQGDSTLELKAGSVLLPRDGVEAELLVNKAFASISSQGVE"
     gene            6924..>7630
                     /locus_tag="PKL93_10205"
     CDS             6924..>7630
                     /locus_tag="PKL93_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ71671.1"
                     /translation="MKFIVIAVSLLSVVLIAPFLIPVRDLEGTVNPILLAEEDSMFVN
                     IKNINIHYKSAGEGSTLVLLLHGFGASTFSWREVIGPLAEEYFVVAFDRPGFGFTSRP
                     LGEDLEIFNPYSMEGQVELTVSLIEYLGYEEAILIGNSAGGLTALEVAASYPQKVKGL
                     VLVDAAVYTNDENNPFFKLLTNTPQGRHLGPLVSRIFLGNSRNLLDLAWYDTGKLTPD
                     ILEGYEKPLKAENWDRA"
BASE COUNT         2158 a         1805 c         1512 g         2155 t
ORIGIN      
        1 agatcaatag gacatttcca atattctttc cagatctttg gttttcaggt taccaggtgt
       61 cacttcgaca agagctccca ttgttctcat agcatcctca gcaagtctac cgagcataga
      121 cttctcgact ccaaggtcct taagcgtaat gtctatatcc acgcttttct gaagcctgtg
      181 tattaattct attgacattt tcgagagctt atcgatatct atctcacttt caggagctcc
      241 cattatttga gctactctgg ccaatctctc ttttgtatga ggccttatgt aatcaaagaa
      301 tgcagggccg gttgcgcaaa gtccagctcc atgggtagcg ttataatggg cgcttatagg
      361 atgctcaatg gaatgatttg cgatgcagcc cgaaagtgtt tcgcaaattc ctgcggctgt
      421 acttgcccat gcgagggctg ttctagcttc aatgtcgttg ccatccctgt aagctatggg
      481 tagataatga tttatgagcg acatggcttc cagagcaaga aggtctgaag ttggctgatg
      541 gtttgtattt atgtaagact caattgcatg atagaaagcg tccattccgg ttgccgctgt
      601 ctgatagggt ggaagggtct tcataacttc gggatcgaca atagaaacag taggaaaagt
      661 ttgatcaaat ccgacaccta tcttctcgtc agtttcagta ttggttacca cggcaaaggg
      721 gtctgactcg gttccggtac cgtgagtagt gggaatagcg ataacaggta gcgccgtttt
      781 gggaattctt cctcccccag atccagtgta atcccagcat ttaccgccat ccttagcgac
      841 cacggctatt agttttgccg agtctatggg gcttccccca ccgagaccga tgatcagatc
      901 gcatccttca ttgtttgcaa actcagcgcc tttatcaacc gtagatgaaa gggggtttgg
      961 gactatctca tcaaatacgg ctgtttctac tccattcttc tctagaacag ctatgaccct
     1021 gtcaagaagg ccagtctttt tcgtactgct cttacccgtt acaattagtg ccttcttgcc
     1081 caaggacttt gcaaactctc caactctgtt taatgatcca acaccgaata ctagccttgt
     1141 cggcaaaaaa tactcgaaat tcgaaatcct atccacttca ttaacctcct caatgtctat
     1201 aaatcgaaaa caaattgttg atttaactca cattacagtg tttcaaccat gaaactcaaa
     1261 actatcgccg gccataagct cccggacttc ggcaggaaat ctttctgcga tcttcttcac
     1321 tacgttgtca cctgtacagt gaccaatatg gaacttatct atatcgaaac ccaatagata
     1381 ttcaaagatc tcttcaagct catcttcagt cttgtgcaac aagtgcaggc ctccaactac
     1441 gttctttact tttccaaatc ccgatgcttg ttttacgatg ttaccaatcc ccctgtgaga
     1501 acagcccgtt ataactgtca caacaccatt ttggacaaca gcaagagaaa gttcgtcttc
     1561 aaagaaatct cgaattcttg tacccccttt gattctgttg tgcttatctc cgggcctttc
     1621 ctcggtaacg aacggagcag cagtcattac gtgtaggcca tttgtgacct ctcttgaagc
     1681 gcgcacagtt tcgatagaag ctcccagctg ctcatattca ctcccacaac ctcctggact
     1741 ggattccgtt gaaccgctga atttcctctc aagacatcct tcgccgacca tgaccttttt
     1801 tgcacctgtt tttttcaaga cacttccgag cccaccaaga tgatcatagt gaccatgact
     1861 gataataatc gactcaacct cagaaagatc tatacccaat ttttcggcat tcttaactgc
     1921 aacgtcggta gatccagtat cgaaaaggaa cttctttcca ttcagttcga taaagaatga
     1981 aattccatgt tcagatttaa aaccttcgag acctttgtca ttgcaaagaa cagttatttt
     2041 catgttaatt ctcctctctg cgaccggggg tttcacctct tccccgagcg acaagtttat
     2101 tcacacttcc gaagtcctaa caagcggaaa aaaccaatca acacgaatct cactaatcaa
     2161 tcaagatcca ttttggaatt taccggtcga tcaaaatctg actctaatca atgctttcaa
     2221 agagatagtt ataagaaatt ccatgaataa cattacaaca atcacgggcc ctggaggcat
     2281 gtcaagcacg tatgatacaa agatgcccat cacgcttgcc actactgcca tagctagtga
     2341 aatcagaatc atacctctga atgaaccggc aatggtcttt gaagtgactg caggaatgac
     2401 caaaaaggca gtgacaagaa ttattcctgc aatttttacc gagcctacta cgctcagact
     2461 tattcctatt agcactccat aatgtatgaa tccaataggt acaccgtagt gttttgccat
     2521 cccctcatcg taagagtaat acagcagttc tcggtaaaac accgcgaaga agacccaggc
     2581 aataacgtta gctatcaata gcatccaaac gtcttcagaa gtgaccataa gtatattacc
     2641 aaagaaaaga cccataacat caggagtata gccccgtata aatgagagag caattacgcc
     2701 cagcgacatc gaaagaggga gaagcattcc aataacacta ctctcggaca gtttgttctt
     2761 cctcgtaaaa taactgacgg caactgcaaa aatcacggcc gtaaccgcag ccatgagaag
     2821 atagttcgtt ccgagcagca gaccaaatgc aataccgccg aacgtcgcgt gagctgcccc
     2881 atcaccaatg aattcaatct ttctaagaac aaccacgctt gaaaaaatac ccgtgagtgc
     2941 acttacaaga actgcggcta gaatagcatt acgcaagaag ctgtatgata tgatatcact
     3001 tataaaatcc tgaatcataa gtgatgctcc ctttcgaaat gatctcttga tcttatgaag
     3061 aaatcaaggt cgctagagaa cgcggactgc agttcctctg gagtaatatt gtccgcatca
     3121 ttatagcaat gtagagtctt gttgatgcac atcacagtat cacaagcgtc aaaaaccatt
     3181 ccaatgtcat ggcttaccat tagtatagca atactctttt cattcttgag agactcgagt
     3241 aacctgtaaa aagaagcttt acccatctca tcaactccag cttccggttc atcgagtata
     3301 agcaagtcgg gatcggaggc caacgccctt gccagcatca gtctctgata ctcaccgccc
     3361 gaaagcgtgc cggcctttct atctctcaga tctaaaatac ctaccgtcct gagaagatca
     3421 tccactctaa tccagtcgct ttttctgaag ggcctcaaga ttccacgatc accgtatagc
     3481 cccattgcag ccacttccct tacctttata gggaaatctc tcttcctgtc tggattctga
     3541 gatagatatc caatcttccc ggaaacaacg atctttcccg aatagccttc aagatctcca
     3601 actatcgctt ttatgagagt tgttttccct gcaccattcg gccctattat cccaacgaat
     3661 tcctttcttg agatactgaa agaaacgtcc ctcagaatcc aatgacttcc aatcctgtag
     3721 ctcaaatttt cgacacttag aattttattt tccatcaaat accctcatca tttcgtcgag
     3781 attccattgt agaagctcaa taatgcttcc cattgagaga cctaaactat ccagttcgcc
     3841 atgaccgacc gctgtttcgc ttttcagtat atctatggct ctctttgaat gctggtactc
     3901 cgcgaatatt ccaatgacat ccttttctct tacaaagtca acgagttcct ttatttcccc
     3961 tatactgggc tcatcgccaa ctccttcaag aacaggctca agctctattc catatcttct
     4021 aaagaagtaa gtgaaacttg ggtgagcaac tattacaatc gaccctcgaa atctctccaa
     4081 ataagagctg gccttctcat cgaaatcatg aagctcggaa attagttttt ctgcgtttac
     4141 tttgtacaac accgagttag ccggatcaat attggacagt ttttccacga taacgggaat
     4201 caagtagtcg gacagaatta ctggatcaag ccagatgtga gggttgaaat ggtgtaagtc
     4261 ggtgttcgct tcaccaattt catcttcaga tacgagaaag tcatccggca actgattccc
     4321 catgtataac acgtgctttc cctgcgctgc cagatcttca agataactct ctaggaaact
     4381 ctccaaatct aaaccattcg caatgactaa gtccgcttca tttagctttc tcacatccga
     4441 tatcttggga gagaatatgt gtgggttctg acctggtcca atcagaagat cgacctggcc
     4501 ttcttgaccg actatttctc taacgatcaa atagtaaggg tttattgttg ccacgatctt
     4561 tagaggcaac agggttgttg aaaacagaat cattagcgct gtgatagtta gtaaagtccg
     4621 ttttttcata gcttcccatc ctcgatgtcg gagagcattt ttctcttgca ctctccacaa
     4681 attccaatga agtaaaacac atgatcagta attgtaaaat cgcggctttc cgcaactttt
     4741 tttgcaaaag aatcggcaaa accctcataa aatggttcgg ttttgtggca ctttttgcag
     4801 tgcaaaaaat gtaccggttc tctttcgcta ctgaaataaa atcttgtctc gcactcgaag
     4861 gatatcgacc ttataagccc ctcatcctcc aggaaggaaa ggcttctata aacgcttgaa
     4921 agattcggtt tttccttcaa ttttccataa atggaatcgg cgctcaacgg cgattcggaa
     4981 ttatttatca ggtccaaaac ttctcttcta agtgatgtca gtcttttcaa ttgacacctc
     5041 cgtattgcaa ttcatttgcg caaactcatt gcaatctatt tttgcatata cttttcttct
     5101 tgtcaattct cttggattct aagactgctt tcgcatcgga agtatttcaa gcagcttttg
     5161 aaagaaaaaa ggcgaggaat ttgcaaattg cgatatcccg gtgatctcta tggccgggtc
     5221 tctcaaaaga atcaaggcct tgccagaatc atcctgccag atctcaccga aaaccctgat
     5281 tttctttctg tacagtcttt gcaaatcttc ggaatcctga ccgaaaagtg tcgcttcctc
     5341 aggccttact ctcacgacaa tatcatcacc aactaccgta aagccattta cgtcaatcac
     5401 agtgaactcg acccatacta tctttccgag aaaaccgccc aattgatacg gataaagggc
     5461 ttcacgaacc tcagaggcat cataaacttg aaaatcattg taggatgaga agatcccact
     5521 tctgttattg aaagcctgtc tgtaggcttc tctgattttc gctgaattat gggaggtatc
     5581 ctcataggtt agaggtctcg ccagaccttg cctgactagt tctgcattca cgaattcacc
     5641 ttcagagttg aagaggtaag caagatgcct tccgtaagaa tcctttccat ggagttcaag
     5701 ggtaaccttt ccattgctca gaaccatctg cttcaagaac tctcttgctt cttcggcaaa
     5761 ttgaccggcc ggcttgcttc ctgaagtgac ctctggtgca tctataccaa tgagcctaac
     5821 cgttccttga agagccttta cagtaataga gtcaccgtca atcacagaag ttactgctgt
     5881 cgttagtttg gaacgatcgc atccttgaag aagaaagaat gagaagaaaa aaacaaggct
     5941 tagaactatg aaaacacttc tcttcactac ttatcccctc tttatcggtt tctaactgtg
     6001 taaaccaatt atacaatcgc actttgatct ctggctttca cagatggaag aatggtcctt
     6061 taatgagcaa atatgtaaat ttgtacttta aaggtagaat agaaacgact gaaaagcaaa
     6121 gggggtgaaa gaatgaaaga caacatttcc tcagagcagc tctcagaact tcttacgcag
     6181 gctttggctc ttatcgagga cggagttgct gtgctcgatg aattctcgaa tgtgcttgtg
     6241 gcaaacagtc gggcctccat tctgatagaa gaggtgcagg aagaagttaa gaagaagata
     6301 agagatttga cacgtagaag tgaagaggct ttggaatacc accacataag aaagaacggg
     6361 agatggttga ggtattattt gaagaaatta tcggtaccaa atgcattgta ctttctcttt
     6421 gtggtcgaag atatctcaga cttgaagcta gccgaacaaa caatttctga acttgctcat
     6481 tttgacggcg agactgggct cccaaattac gtgcttttca gagacagatt gaacatggca
     6541 atatttagaa gcagaagaaa caacctgttt tcgatggtcg ctgcatttga actaagtaat
     6601 agagaagcag ttcagagcat ccaagaagca acgcttgtag agataacaga agagcttgtc
     6661 aagggcataa gaaagagcga tacgctgtgt aggttttcta gaagagaatt tcttctgctt
     6721 gccgaggacc ttaaaaatcc tgattccgcc gccaggataa ttggaaaagt ccttagctct
     6781 tttgagaagt ggaaattgga ccaaggcgat tcaacgctgg aactcaaagc gggatctgtc
     6841 ttgctcccaa gggacggagt ggaagcggag ttgcttgtca acaaagcctt cgcttcgata
     6901 agttcgcaag gagtcgaata gccatgaaat tcattgtcat tgcggtttct ttgctctcgg
     6961 tagtcttaat tgctcccttt cttatccccg tgagagatct ggagggtact gtgaatccta
     7021 ttctacttgc agaggaagat agtatgtttg tcaacataaa gaacataaac attcattaca
     7081 agagcgccgg tgaaggttct actttggtcc ttcttcttca tgggtttggg gcaagcactt
     7141 tttcgtggcg cgaggtgatt ggtcctttag ctgaggagta tttcgtagtt gcgttcgaca
     7201 gacctggatt cggttttacc tcaagaccgc tgggtgaaga tcttgagatt ttcaaccctt
     7261 attcaatgga aggccaggta gaacttacag tatcactcat cgagtatctg ggatatgaag
     7321 aagctatctt gatcggtaat tctgccggcg gccttactgc actggaagta gcggcatcgt
     7381 acccacaaaa ggtcaaagga ctcgtgctag tggatgctgc cgtatataca aacgatgaaa
     7441 ataacccatt tttcaagtta ctgacaaaca caccacaggg tagacatcta ggtcctctcg
     7501 tatcgagaat tttcctggga aactcacgaa atcttctaga tctggcttgg tacgataccg
     7561 gcaagcttac cccggatatt cttgaaggat acgaaaaacc actgaaggcg gaaaactggg
     7621 acagggcttt
//
LOCUS       DAOQYI010000069         7390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_10427_length_7390_cov_73.356237, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000069 DAOQYI010000000
VERSION     DAOQYI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10427_length_7390_cov_73.356237"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..2924)
                     /gene="uvrA"
                     /locus_tag="PKL93_10210"
     CDS             complement(87..2924)
                     /gene="uvrA"
                     /locus_tag="PKL93_10210"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNQ71672.1"
                     /translation="MDRYIYVKGARVHNLKNVNVAIPKNSLTIITGLSGSGKSSLALD
                     TIYAEGQRRYLESLSTYARQFLGEMKKPDVENIEGLSPAIAIEQKTVSHNPRSTVGTV
                     TEIHDYLRVLFARVGIPHCPSCGRVVQKQSLDEIVEGVLKEYGAEDRIAIYAPVANGK
                     KGEFKKELEALRKKGFVRIEIDGETYDLDEDLKLDKNKRHTINLVVDRLRADADNASR
                     LADSIEMALHEGDGFVEVGLVDSEERKIFSENFACPDCGISLPEISPKIFSFNNPFGA
                     CPKCHGLGYTMEFSKDLLVDESLSVLKGAIKTMSSNRDTFTLKMIVKVIESLGDDPAK
                     PFKDLRDDVKNAILFGTDSDISMKYKSDRLTYELKRPYEGIINNLRRRYNETQSEDMR
                     FWMESNFMVQQICTQCNGQRLRPEALAVTLGGLNIAQISDLTIQEIYDFMTGIHFTDF
                     QREIVGELLSEIEKRLKFLVDVGLDYITLSRYAMTLSGGESQRIRLATQIGSGLTGVT
                     YVLDEPTIGLHSRDNDRLIKTLKNLRDLGNTVIVVEHDEEVIRSSDYIVDVGPGAGVH
                     GGEIVYQGTTQALLDNPPERSLTGKYLNGELSVPRFESANNSNGKFLTVKGAEKNNLK
                     HIDVSFPLGKFITVTGVSGSGKSSLVMDTVYPSLKVALGGSNSSSEKLAELLGWEEID
                     NVIVIDQNPIGRTPRSNPATYTGLFDHVRDLFAKTQEARARGYNKGRFSFNVKGGRCE
                     ACQGHGLIKIEMQFLPDVYVECDVCKGRRYNKETLEIRYRGKTISDILNMTVEEALTF
                     FERIPTLNRILGLLNDVGLGYIRLGQPATTLSGGEAQRIKLASELKKTSTGRTFYILD
                     EPTTGLHFDDVAKLVQVLKRLVEKGNTVVVVEHNMDVVKNADYIIDLGPEGGANGGEV
                     VISGTPEEVAEYSHSHTGYYLKKQLSQLEAL"
     gene            complement(2917..4233)
                     /locus_tag="PKL93_10215"
     CDS             complement(2917..4233)
                     /locus_tag="PKL93_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNQ71673.1"
                     /translation="MKKLFGTDGIRGVINEELTPELAMKLGNAIGRYYTGKYNRFIIA
                     KDTRSSGDLLESAMAAGAASAGMNVEFAGVIPTPALAYITNKEGTLGAVISASHNPAV
                     YNGIKVLARGMKISDEDEVEIENLIIDTPFHYTVYSGVGKISYKDHYRDEYIDYVIGL
                     YRNERLPSDGIVVDGANGAISTVISMVYESLGIGAELREIEPNGININDKCGSLFPNF
                     LGDSLKKGQIGVLFDGDADRCLFVLPGSKLIDGDMLMALNSRKLVNQGRLKGNRVVAT
                     VMSNLGFEKYLTSKNISLDRTKVGDKYVLERMLQTGGVLGGEQSGHIIFLDRSTTGDG
                     LITSLETLNSLEELDESLEEFAASFPVYPQLLKNVPVSNKKLVMEDMNLKNRLEELKQ
                     NDDLRIVLRPSGTEPYIRVMVEGAEQLLVEEICQELVELVQECSNG"
     gene            4333..4423
                     /locus_tag="PKL93_10220"
     tRNA            4333..4423
                     /locus_tag="PKL93_10220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4367..4369,aa:Ser,seq:gga)
     gene            4502..5299
                     /gene="rsmA"
                     /locus_tag="PKL93_10225"
     CDS             4502..5299
                     /gene="rsmA"
                     /locus_tag="PKL93_10225"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNQ71674.1"
                     /translation="MGERSINIRLNKALGQNFLKSDRVSRRIVESVELNPSSTIVEIG
                     VGSGSLTSILLEKGLRVIGFEIDTRFSEGNRRLEGERCELRYEDFLKADLSTLPDSVT
                     YVANIPYYITSPIIERIMFDGPSFDRAVLMVQKEYADRLTATPRTKEYGILTVNVNTF
                     SEVRELFQVSRKEFIPQPEVDSMVIELSLLENPPIGEARRDQYRKFVRHCFSQRRKKL
                     KNNLKSIVDSPEELLGSMAIGIDVRAEELSVDDFVNLFKNIYPEGGV"
     gene            5317..7026
                     /locus_tag="PKL93_10230"
     CDS             5317..7026
                     /locus_tag="PKL93_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNQ71675.1"
                     /translation="MERELFERILNSLIEGIIVVDSDERIAFINDEALEILGVNGEEA
                     TGALVRETIPNTRLHIVLKSGEPELNRIQYLKEKTIVTSRFPIVNSQGEVHAAMAVFR
                     DITNVEKMAEEVINHKEMEGLLTSIIDATYDAISVADEKGKIVLVNRAYTRITGMSPD
                     FVIGKMATVDIAEGSSLHLEVVRTRKPIYNARLKVGPNRKEVLVNVTPLFVKGEFKGS
                     VGVVHDISEIERLARELEDTKRLLRHMKAKYTFDDIAGSSKLMQVAIDQARKVSKTRA
                     TVLLNGQSGTGKELFAHAIHNSSDRREQSFISVNCAALPENLLESELFGYVEGAFTGA
                     RRGGKKGLLQEANGGTVFLDEVAKMSLSVQSKFLKFLQDREIKPVGGNQTYKLDVRVI
                     AATNVSLEKLVASGEFLPDLYYRLNVVPIVIPPLKERPEDIPEIARVVMMRLNQEYGR
                     MVTEIDPAVLRMFEKHDWPGNVRELENVIGRAMISMELEEKTIRLSHIKGILEKPDKR
                     ERLPEGSLSALLDDYEKEIIKDSLQRNDWNKTKTSNELGISIRSLYYKLEKHGITDTH
                     ENR"
     gene            7040..7390
                     /gene="ssrA"
                     /locus_tag="PKL93_10235"
     tmRNA           7040..7390
                     /gene="ssrA"
                     /locus_tag="PKL93_10235"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1899 a         1745 c         1790 g         1956 t
ORIGIN      
        1 gactggcgtg cgtcagcaga ctggcttcga aatcgcttgt tccctaaagt agcatcacac
       61 ctaggccgat agtctccggt gtagaactaa agagcttcaa gttgtgagag ttgtttcttg
      121 agatagtatc cggtatgact gtggctatat tcggccactt cttcgggggt tccggaaatc
      181 acgacttctc cgccgtttgc accgccttcg ggtcccaaat caattatgta atcggcgttt
      241 ttgacgacgt ccatgttgtg ttcgacgact acgaccgtat tgcccttttc gactaggcgt
      301 ttcaaaacct gtacgagctt tgcgacatcg tcgaaatgca gcccggttgt cggctcatcg
      361 agaatataga aggttctgcc cgtggaggtc ttcttcagtt cagatgcgag ttttattctc
      421 tgagcctctc cccccgaaag cgttgtagcg ggctgaccaa gtcgtatgta tccaagtccg
      481 acgtcattca gaagtccgag gattctgttt agtgtaggta ttctttcaaa gaatgtcaag
      541 gcctcttcga ctgtcatgtt taaaatgtcg gaaatagtct ttccgcgata gcgtatctcg
      601 agagtctctt tattgtatcg cctgcctttg cagacatcgc actcgacgta gacatcggga
      661 aggaactgca tctctatctt tataagccca tgcccttgac aggcctcgca tctcccgcct
      721 ttgacgttga aggaaaaacg ccctttgttg tagccccttg ctcttgcttc ctgagtcttg
      781 gcgaagagat cccttacgtg atcgaagagc cccgtgtatg ttgcaggatt ggatctcggc
      841 gttctgccta ttgggttctg gtcgatcact attacgttgt cgatctcctc ccagccgagt
      901 agctctgcca gcttttcgga ggaggaattg gatccaccca gtgcaacttt gagtgaaggg
      961 tacaccgtgt ccattacgag agagctcttg ccacttccag atacaccggt taccgttatg
     1021 aatttgccta gcggaaatga aacgtcaata tgcttaagat tgttcttctc cgcgcccttt
     1081 acagtgagaa actttccatt tgagttgttc gccgattcga atctaggaac ggataactcg
     1141 ccatttagat actttcccgt aagagatcgt tccggcgggt tatcgagaag cgcctgagtt
     1201 gtcccctggt aaacgatctc tcctccgtga actcctgctc ccggtcccac atccacgatg
     1261 tagtcggagc ttcttatgac ctcttcgtcg tgttcgacaa ctattacggt gtttcccaga
     1321 tccctgaggt tcttcagtgt ctttatcaac ctgtcattgt ctcttgaatg tagccctatg
     1381 gttggctcat cgagcacata ggtaaccccc gtaaggccgg aaccgatctg tgtcgcaagt
     1441 cttattcttt gcgattcccc tcctgaaagc gtcatggcgt atctggacag ggtgatgtag
     1501 tcgagtccca catccaccag aaatttgagc cgcttctcta tctcactcag gagctctccg
     1561 acgatttccc gctgaaaatc cgtgaaatga atacctgtca tgaagtcgta gatttcctgg
     1621 attgtgagat cggatatctg cgcgatattc aaaccgccaa gagtaactgc aagcgcttcg
     1681 ggtctcaaac gttgaccgtt acactgcgta cagatctgtt gaaccatgaa attgctttcc
     1741 atccagaagc gcatgtcttc cgactgtgtt tcgttatatc gtctccttag attgttgatt
     1801 atgccttcat agggtctctt aagctcgtaa gtgagccgat cggacttgta tttcatagaa
     1861 atgtcgctgt ccgttccgaa aaggatggca ttcttcacat cgtctcgaag atctttgaag
     1921 ggtttggcag gatcgtcgcc tagcgattct attactttta cgatcatctt cagggtaaat
     1981 gtgtccctgt tgcttgacat cgtctttatg gcgcccttca aaacgctcag actttcatct
     2041 acaagaagat cttttgagaa ttccattgtg taaccaaggc catgacactt tggacaggcc
     2101 ccgaaagggt tgttgaatga aaagatcttc ggcgaaattt ccggaaggct tatcccacag
     2161 tcgggacagg caaaattctc actgaagatc tttctctctt cggaatcgac cagaccgact
     2221 tcgacgaagc catccccttc atgcaaagcc atctcgatgc tgtctgcaag acgtgaagcg
     2281 ttatcggcgt ctgccctcag tctatctacc actaggttta tcgtgtgtct tttgttcttg
     2341 tcaagtttga ggtcttcgtc gagatcgtat gtttctccat ctatctcgat ccttacaaag
     2401 cccttctttc tgagagcttc aagttccttc ttgaactcgc ccttcttacc attggcaaca
     2461 ggggcgtaga tggcgattcg atcttccgca ccgtattcct tcagaactcc ttctacgatt
     2521 tcatcgagac tctgtttctg aactacgcgc ccacaggacg gacaatgtgg aatacccact
     2581 ctggcgaaca aaactctcag gtagtcgtgt atctccgtaa cggttcctac cgtcgatcta
     2641 gggttatggc taacggtctt ctgctctatg gcaattgccg gagagagccc ctcgatattt
     2701 tctacatcgg gtttcttcat ctcccccaga aactgtctag cataagtgga aagagattcg
     2761 agatatctcc tctgaccttc tgcgtaaatt gtgtcgagtg ccagcgagga ctttcccgat
     2821 ccggacaggc cggtaataat agtcaaggaa ttcttgggga ttgcgacatt gacatttttc
     2881 agattgtgta ccctggcgcc tttcacatat atatacctat ccattactac actcctgcac
     2941 taactcgacc agctcctgac agatctcctc gaccaacagc tgctcggctc cttcaaccat
     3001 gactctaatg tacggttccg tgcctgacgg cctgagaacg attcgaagat catcattctg
     3061 cttcagttct tcgagtctat tcttaagatt catgtcttcc atgaccagct tcttgtttga
     3121 tacgggaacg ttcttcaaca actgtggata gacggggaaa gaggcagcaa actcttcgag
     3181 gctttcgtca agctcttcga gtgagttcaa tgtttcaagt gaagtgatga gaccatcgcc
     3241 ggtggtactc ctgtcgagga agatgatatg accagattgc tctccgccaa ggactccgcc
     3301 tgtctgcagc attctttcga gaacgtattt gtcgccaacc tttgttctat ccagcgagat
     3361 gttcttcgag gtgagatact tctcgaagcc gaggtttgac attacagttg caacgaccct
     3421 attgcccttc agccgtccct ggttcacaag ctttctcgag ttgagcgcca tcaacatatc
     3481 gccgtctatc aacttggagc ccggaagaac gaatagacat ctgtctgcat ctccatcgaa
     3541 aagaacccca atctgtcctt tctttaatga atcgcccagg aagttcggaa atagcgatcc
     3601 gcacttgtcg ttgatgttaa tcccgttcgg ttcgatttcg cgaagctccg cacctatacc
     3661 taggctttcg tataccatag atatgacggt tgaaattgct ccgttggcac cgtcgacaac
     3721 gatcccgtca gaagggagtc tctcattcct gtaaagacct ataacataat cgatatactc
     3781 atctctatag tgatctttat aactaatctt gccgactcca gaataaactg tatagtgaaa
     3841 cggggtgtcg attatgaggt tctctatctc cacttcgtct tcgtcggata tcttcattcc
     3901 cctggcgaga accttgattc cgttgtaaac agcgggatta tgggaagcag aaataacagc
     3961 tcccaacgtt ccctctttgt tcgtgatata cgctaatgct ggagtcggga ttacgcccgc
     4021 gaactcgacg ttcatcccgg cggaggcggc tcccgcggcc attgcgctct ctagaagatc
     4081 accggaactc ctcgtgtctt ttgctatgat aaacctgtta tactttccag tgtagtatct
     4141 gccaatggca ttgccaagct tcattgcaag ttcgggtgtg agctcttcgt taataacgcc
     4201 ccttatcccg tcggttccga acagcttctt cattaagaca cccccaaata taaacatttc
     4261 tatacctagc atttagttta ccacagaatc tcgttgacaa ctcaaactgc gaactatata
     4321 attgtgactg ttggagagat ggccgagcgg tcgaaggcgt acgcctggag agcgtatgac
     4381 gagcacaact cgtccgtggg ttcaaatccc actctctccg ccagaaaacc acccgtcttt
     4441 ccgacgggtt tttttgctta gatgggagag ttattgagag cctatctgaa acggaggagc
     4501 aatgggagag agatcgataa acataaggct aaacaaggcg ctggggcaga acttcctgaa
     4561 gtcagacaga gtctctcgca ggattgtcga atcagtggaa ctgaatccgt cgtcgactat
     4621 agtcgaaata ggtgtaggat cgggttcgct cacttctatc ctgttggaga aaggcttaag
     4681 agttatcggg tttgaaatag acacaaggtt ctccgaaggc aaccgaaggc tggagggcga
     4741 acggtgcgag ctccgttacg aggactttct caaggccgat ctatctactt tgcccgactc
     4801 tgtcacgtat gttgccaaca ttccttatta cattacctct cccattatcg agagaataat
     4861 gttcgatggg ccatcatttg acagggctgt gctaatggtt cagaaggagt atgcggatag
     4921 attgaccgca acaccccgta cgaaggaata cggtatactg acggtgaatg ttaatacttt
     4981 ttccgaagtg agagagcttt ttcaggtttc tagaaaggag ttcattccgc agccagaagt
     5041 ggactcgatg gtgattgagc tgtcgttgct tgagaaccct ccgataggag aagctagaag
     5101 ggatcagtac aggaaatttg tcaggcattg tttctctcag agacggaaga agctgaagaa
     5161 caatctgaaa agcatcgtgg actcaccaga ggaattactt gggtcaatgg ctataggaat
     5221 cgacgtcagg gcagaagaac taagcgtaga tgatttcgta aacttgttca agaacatata
     5281 tcctgaaggt ggtgtgtgac cgaaagaggt caagcaatgg agagagagct tttcgaaagg
     5341 attctgaatt cgctgataga gggaataata gttgtcgatt cagacgagag gatagccttc
     5401 ataaacgacg aggccctcga gattctagga gtgaatggag aggaagcgac cggtgctctc
     5461 gtaagagaga ccatacctaa cactaggttg cacatcgttc tgaagagtgg tgagccggag
     5521 ctcaacagaa ttcagtatct caaggaaaag acgatagtaa cttcaagatt ccccatcgtc
     5581 aactcacaag gtgaagtcca tgctgcaatg gctgtatttc gtgacataac aaatgttgaa
     5641 aagatggctg aagaagtcat taaccataag gagatggagg gtctattgac ctccatcata
     5701 gatgcaacat atgatgcaat ttcggtggcc gatgaaaagg gaaagatcgt tctcgtaaac
     5761 agggcctaca ccagaataac aggaatgagt cccgatttcg tcattggaaa aatggccaca
     5821 gttgacattg cggaaggttc ctctcttcat ctggaagttg taagaacgag gaagccgata
     5881 tacaacgcaa ggctgaaagt cggtccgaac agaaaggaag tgctggtcaa cgtaactccg
     5941 cttttcgtga agggtgagtt taagggaagc gtgggtgttg ttcatgacat ctccgagata
     6001 gagagactcg cgagggaact ggaggacacg aagcgcctcc ttcgacacat gaaggcaaag
     6061 tatacttttg acgatatcgc cggttcgtct aagctgatgc aggtagcgat tgatcaggcg
     6121 agaaaggttt caaagacaag agcaacagta ctgctgaacg gtcagagcgg aactggaaaa
     6181 gaactcttcg cgcacgcaat tcacaattcc agtgatagac gagaacaaag cttcataagc
     6241 gttaactgcg ctgccctgcc ggaaaacctc ctcgaatcgg agctcttcgg atatgtcgaa
     6301 ggtgctttca caggagcccg gagaggggga aagaagggtc ttcttcaaga ggcaaacgga
     6361 ggaaccgtat ttctcgatga agttgctaag atgagtctct cagttcagtc aaaattcctg
     6421 aagttccttc aagataggga gataaagccg gttggtggaa atcagaccta caaactcgat
     6481 gtcagagtaa ttgcggcgac caacgtgagt ctggagaaac tcgtcgcgtc cggtgaattc
     6541 cttcccgacc tatactatcg tctgaatgta gtgccaatcg taattcctcc gctcaaagag
     6601 aggccagagg atattcctga gatagctagg gtagtgatga tgaggctaaa tcaggagtac
     6661 ggaaggatgg ttacagaaat cgatcctgca gttctgcgca tgtttgagaa acacgactgg
     6721 cctggaaacg taagagaact tgaaaacgtc atcggaaggg caatgataag tatggaacta
     6781 gaagaaaaaa ctatacggct ttcgcatata aagggaattc ttgagaaacc ggacaagaga
     6841 gagaggctac ccgagggaag tctatcagcc cttctggatg actacgaaaa agagataata
     6901 aaagactctc ttcaaagaaa cgattggaac aagacgaaga cttcgaacga acttgggata
     6961 agcataagga gtctatatta caagctggaa aaacacggta tcactgacac tcatgaaaac
     7021 cgatgatata attatcaatg ggggcgaaac ggtttcgacg gggtcaatga acttgtagga
     7081 gcgagccgag gtcccggagc cgcgttaaaa aaccggaaaa gcagaaatgc caataacgaa
     7141 tttgcacttg ctgcttaata attaataagt agccgtccga atagcttttt ggtctggtgg
     7201 gctatgaagg gcgtcagaaa atcagacctt gcccgtgaat atgctccgta ttcccgggga
     7261 cacttaggag cttggacagt cggactctgc ctgtgaagtc ttgactgttg acgacgatca
     7321 caggatgcgc tcggagagcc tgcaaggaat ttggtttcgg acgcgagttc gactctcgcc
     7381 gcctccacca
//
LOCUS       DAOQYI010000070         7310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_10577_length_7310_cov_81.119090, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000070 DAOQYI010000000
VERSION     DAOQYI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10577_length_7310_cov_81.119090"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1135
                     /locus_tag="PKL93_10240"
     CDS             233..1135
                     /locus_tag="PKL93_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ71676.1"
                     /translation="MQIKLRYLLVFILPALILYSIFIIYPLFDSLILSFFSWSGVGPR
                     TFVGLKNFQTLFSGSFSKELFNAFFHNVYFFIMLTILELGLGFLIALMLASKIKGAGV
                     FRVVTYIPNMIPLVLVGFMWVLFLNPQAGLVNQLLRTVGLGSLAQPWLGQANTALTTI
                     ILVNVWRNLGFYVLVILAAILDISPELIEAAYIDGANNWRITWRIIFPLAFSTFRTLA
                     ILLFIWSFNIFDMVYALEGVQAGPYRSTDVLGTLFYRTAFGGLGSGAVDMGLGASVTV
                     FIFAIVMPVSILYVFIVEKRQRSS"
     gene            1139..1966
                     /locus_tag="PKL93_10245"
     CDS             1139..1966
                     /locus_tag="PKL93_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ71677.1"
                     /translation="MLKLSLFGKIVVYFLLVVYCLIILVPFFIMIMNSLKSMREIYLQ
                     PFSFPSKVLFENYSKAWQQAGIGTGYKNSLIVAGVSVIGIVIVSSMFAYAISKYTFKG
                     RRFLFIYSMLGLAFPARLAIIPIFILMRNFDLTNSLLGLIIIYTSINIPFSVFLLKNF
                     IDGVPNELSEAARIDGASPMQIYWRIILPLVKPALSIVSIVSFVNVWNDFFFPLIFIT
                     DRSKATITLAVSIFFGEYVNQWHLLFSGLTLAVAPTIILFLLFSRQFITGMTQGAIK"
     gene            1977..>2599
                     /locus_tag="PKL93_10250"
     CDS             1977..>2599
                     /locus_tag="PKL93_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNQ71678.1"
                     /translation="MKVKLGIDCLLESDILKKKRIGLLTNSSGVNGDLKINVDLMLNG
                     GFKIVKLFGPEHGISGAAADGVSVDNAVDPKYGIPIYSLFGDVIRPTEEMLSGIDVFV
                     YDIQDVGLRFYTYLYSLVYSMEECAKRGIEVVVLDRPDPLSCKIVGPTIKKRLDSIVG
                     GYGLALRYGMTVGEVAKYFNKVFDIGADLTVVPMENYEKYMYYDETG"
     assembly_gap    2600..2699
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2700..3013
                     /locus_tag="PKL93_10255"
     CDS             <2700..3013
                     /locus_tag="PKL93_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNQ71679.1"
                     /translation="QPCNGLEFYVTDRNAIKPLELALDIIATLMKLWPEEFDWDVHYH
                     GTSIPLNKMWDGRYHFDLIMGDEQYRERLLEGATAAELSGLWKDEQREFENLVREFRI
                     Y"
     gene            complement(3000..3866)
                     /locus_tag="PKL93_10260"
     CDS             complement(3000..3866)
                     /locus_tag="PKL93_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNQ71680.1"
                     /translation="MSRKIIAMDGGGTNLRVTCISSEARFRKNYGTGVNITAVSSANL
                     VSIFSLVRDDVGIPDYIVAAFSGAGDKTREEKLRQALDSVFGNIEIEVIMDIEGLYRA
                     AVGEARGVVVVSGTGSTVYGHDREGKAVKSGGWGHIFDDEGSAYWISKELITASLKYR
                     DGLIPHDPIFDKLVEFFEMSTIEELVNLTLMPDMKTEIASFSRVALENPIGLARRIAD
                     EGIEILAKRTLNVLEKTGEVETIHTFGGSFASAYYNNKYSEMINNHRTTLFTGNVDEI
                     LAEQAFEKLNRS"
     gene            complement(3863..4588)
                     /locus_tag="PKL93_10265"
     CDS             complement(3863..4588)
                     /locus_tag="PKL93_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ71681.1"
                     /translation="MIEAGAPVYYRIYKELKRRIGKGVYSKRLPTEKELCNEFNVSRL
                     TLRRALDELKRESVIESAKGRGTFIVDEKREESISSLTGFTEEAARDKQRARSVVLSD
                     GLMVPPEEIAKLFGIPDGAMVVLLERVRYLNDEPYGIERAYLNPLADIRILNIVNRDM
                     SEASLYNILKNELSLVLDHALENIEVCRLTKEEAKHLKVNENSYGIRRERQTFTDKNI
                     CVEVVRSVYRGDRYRLKVVRRVE"
     gene            complement(4585..5493)
                     /gene="murQ"
                     /locus_tag="PKL93_10270"
     CDS             complement(4585..5493)
                     /gene="murQ"
                     /locus_tag="PKL93_10270"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNQ71682.1"
                     /translation="MKFDQLETERSNPRTRNLDELSTYEMLRIMNEEDATVPLSVREV
                     LNEVASAVELCVRSLEMGGRIVYAGAGTSGRLGVLDAAEVVPTFGVSKDLFVTMIAGG
                     QEALANAVEQAEDSIELGEEEAKKVDITEKDAVVGISASGRTPYVEGILKYARKQGAR
                     TILICNVGTPRLTELADVTIAVRTGPEVITGSTRLKAGTAQKMVLNMISTVTMVKVGR
                     VFGNYMIGVKILNRKLVDRATRIVSEISDLSYEESEKILEASGREVPLAILMALTGLG
                     KEKCEESLKRNRGHVRSALKELGGVS"
     gene            complement(5495..7033)
                     /gene="nagZ"
                     /locus_tag="PKL93_10275"
     CDS             complement(5495..7033)
                     /gene="nagZ"
                     /locus_tag="PKL93_10275"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNQ71683.1"
                     /translation="MNFDEAVGRLFLIGIPGPEIDSETERTLREVKPGAVILFSKNIV
                     DAVQVRALVDDIQRVLGYKPAIAIDQEGGIVSRLRDGFSVSPGAMAIAATGSTKNSFV
                     VGRILAREMNAIGVNWNLAPVVDINCNPKNPGIGVRSFGDSPDQVIAYARAFVEGMKS
                     ERVMSCLKHFPGKGRVDVDAHLDLPILDIPQSLLYEYELRPFKEIQADSIMPSHIYMP
                     QLQASRVPASMSREILTDLARNVLNYRGVLVADDLGMGGVSNYFTPEEAAIQGLRNGM
                     DYLTYCHDPVVQRRVKKILIREIEKSPELEKRFQESLQRVEKFRVEATSADRFSIEEI
                     ASQESLNAMQEISDRSITAILEDSSLLPLPIESVSGIFSVRLSRLVQVEDGPQKGVPT
                     VAREIAQMADCPIIDFSPNITVEEADRLAADSPGKGVMLVFTENAHLHEGQRKLLLKL
                     SQRTGRTLVIALRNPYDAFLEGVKNCIISYGYEAVSQRSLKKILDGTIKAQGKLPIKI
                     PQEV"
BASE COUNT         1665 a         1869 c         1650 g         2026 t
ORIGIN      
        1 tgggtctact gggtaggatc tgtcttcacg acacagaagc cttctctgta cgacgacgtt
       61 ctcagcccgg gaatgcaagc tctttacgca ggccagctta cccccgaagg actctctcag
      121 atggctcagg atgcaatatc tcagtggtac ccacctttga tgaatcagta aacgatagat
      181 ttcttctctt ccccgccggt aggcggggag gagtctatag aggagaacga agatgcaaat
      241 aaaactcaga taccttctcg tcttcatttt acctgctcta atcctgtatt cgatattcat
      301 aatctacccg ctgtttgaca gcttaattct gagcttcttc agctggtcgg gcgtggggcc
      361 gagaaccttc gtcggactga agaactttca gactctcttc tccggaagct tttccaagga
      421 gttgttcaac gcattctttc acaacgttta cttcttcata atgctcacga ttctcgaact
      481 tggactgggc tttttgatcg ctttgatgct ggcaagcaag ataaagggag ccggagtatt
      541 cagggtagtc acgtatatcc cgaatatgat accccttgtt ctggtcggct tcatgtgggt
      601 tctttttctg aatcctcagg ccggactggt gaaccagctc ttgagaactg tcggacttgg
      661 gagtttggcc caaccctggt taggtcaggc aaacacagct cttacaacaa taattcttgt
      721 aaatgtgtgg cgaaatctcg gattctatgt gcttgtaatt ctggcggcga ttctggatat
      781 ttcacccgaa ctgatagaag ccgcctatat cgacggagcg aacaactgga ggataacctg
      841 gagaatcata ttcccgctgg cattctccac attcaggacg ctagcaattc tcctcttcat
      901 ctggagtttc aatatattcg acatggtata tgctctggaa ggcgttcaag ccggtcccta
      961 cagatctaca gacgttctcg gaacgctttt ctacagaacg gccttcggtg gacttggtag
     1021 cggagccgtt gatatggggc tgggggcttc ggtaacggtc ttcatttttg ccatagtcat
     1081 gcccgtctct atattgtatg tcttcatagt cgagaagaga caaaggagtt catgagtcat
     1141 gctgaagctt agccttttcg gtaaaatcgt agtctacttc cttttggtag tttactgttt
     1201 gatcattctg gttccgtttt tcattatgat aatgaactct ctcaaatcaa tgagagagat
     1261 ctatttgcaa ccattttcat tcccgtcgaa agtcctcttc gaaaactatt cgaaggcatg
     1321 gcaacaggcc ggaataggaa cgggttacaa gaacagtttg atcgttgccg gggtatccgt
     1381 catcggtatt gtaattgtct cttcgatgtt cgcctacgcg atttcgaagt acactttcaa
     1441 aggaagaaga ttcctcttta tttactctat gctgggtctg gcttttccgg cgagactcgc
     1501 gattatcccg atattcatac tgatgcgaaa cttcgatctg acgaactcgc tactgggact
     1561 gataataatc tacacttcaa tcaacatccc gttctccgtc ttccttctga agaattttat
     1621 tgacggagtc cctaacgagc tttcagaagc tgcaagaatc gacggtgcct ctccgatgca
     1681 aatttactgg agaataattc ttcctctggt caagcctgcc ctgtcgatag tctcgatcgt
     1741 cagcttcgtc aacgtatgga acgacttctt cttcccgctc atcttcatca cggatagatc
     1801 gaaggcgaca atcactctgg cagtttcgat cttcttcggg gagtatgtga accagtggca
     1861 tcttttgttc tcgggactta cgctggccgt cgctccaacg ataatactct tcctgttgtt
     1921 ctccaggcag ttcataacgg gcatgacgca aggagcaata aagtgaggtg ctgggtatga
     1981 aagttaagct tggtatagac tgtctcttag agagcgacat actgaagaag aaacggatcg
     2041 ggttgctgac gaattcgagc ggcgtaaacg gagatctgaa aatcaacgtc gatctcatgc
     2101 tgaacggggg attcaaaatc gtgaaacttt tcggacccga gcacggtatc tcgggagccg
     2161 cagccgatgg agtgagcgtc gataacgcgg tggatccaaa gtacggtata ccgatttact
     2221 cgctcttcgg agatgtgata agaccgacgg aggagatgct ttccggaatc gatgtcttcg
     2281 tttacgatat tcaggacgta ggtcttagat tctatactta tctctactcc ctcgtctatt
     2341 caatggagga gtgcgctaaa agggggatag aggtagtcgt cctcgataga ccagatccgc
     2401 tttcatgcaa gatcgtgggt cccacaataa agaagaggct tgacagcatt gtcggcgggt
     2461 acggcctggc tctgagatat ggcatgaccg tcggagaggt ggcaaagtac ttcaacaagg
     2521 tcttcgatat tggcgccgat cttacagtag ttccgatgga gaattacgag aagtatatgt
     2581 actatgatga gaccggtcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2701 ccagccctgt aatggcctcg agttctatgt tactgataga aacgcaatca aacccctgga
     2761 acttgcccta gatattatcg ccactctcat gaagctttgg ccggaagaat tcgactggga
     2821 tgttcattat cacggaacca gcatcccact gaacaagatg tgggacggca ggtaccattt
     2881 cgacctgatt atgggcgacg agcagtatag agaaaggctc ctggaaggag ccacggccgc
     2941 cgaactttcc gggttgtgga aagacgagca gagagagttc gagaatctag taagagagtt
     3001 caggatctat tgagcttctc gaatgcctgc tcggcaagga tctcatcgac attgcccgtg
     3061 aagagcgttg tcctgtgatt gtttatcatc tctgaatact tgttgttata gtaggccgag
     3121 gcaaaggaac ccccaaatgt atggatggtt tctacctcgc ctgttttttc caggacattg
     3181 agtgttctct ttgcgagtat ctctattcct tcgtcagcga ttcttcttgc aagcccgatg
     3241 gggttttcaa gcgccactct gctgaaggac gctatctctg tcttcatgtc gggcatgagg
     3301 gtgaggttta cgagctcttc aatagtactc atttcgaaga actcgaccaa tttgtcgaag
     3361 atggggtcgt gaggaatcag gccgtcccta tacttcagag aggcagtgat caattcctta
     3421 gaaatccagt aggcgcttcc ctcgtcgtcg aatatatggc cccagccgcc ggacttgacg
     3481 gcctttcctt cccggtcatg gccgtagact gtcgatcccg tacccgaaac gaccacgaca
     3541 cctctagcct ctccgacggc ggctctgtac agaccttcta tatccatgat aacttcaatc
     3601 tcaatattgc cgaaaacaga atccagcgcc tgtctcagct tttcttcgcg agtcttgtct
     3661 ccagcgccgg agaaggccgc gactatgtaa tcgggaattc caacgtcgtc tcgaaccaac
     3721 gagaagatcg atactaaatt cgcgctactg acagccgtaa tattgactcc ggtgccatag
     3781 ttcttcctga acctggcctc gctggatatg caagtgactc taagattcgt cccgcctcca
     3841 tccatcgcta ttatctttct gctcattcta ctctcctcac aactttaagt ctgtaacggt
     3901 cgcctctgta tacggatctc accacttcaa cacagatgtt cttgtcggta aatgtctgtc
     3961 tctcgcgtct tattccatag ctgttctcgt tgaccttgag gtgcttcgcc tcttctttcg
     4021 tcagtctgca gacctcgatg ttctccaacg cgtgatcgag aaccagtgaa agctcgttct
     4081 tcaggatatt ataaagcgat gcctcgctca tatctctgtt cacgatgttg agaatcctga
     4141 tatctgcgag cgggttcagg taagcccttt caattccgta gggctcgtcg ttgagatatc
     4201 ttaccctttc cagcaacaca accatcgcgc catcgggaat tccgaagagc tttgcgatct
     4261 cttccggcgg aaccatcaac ccatcgctca ggacaacgga tcttgccctc tgcttgtctc
     4321 gggcggcctc ctcggtgaac cctgtaagag agcttatgct ctcctctcgc ttctcgtcga
     4381 caataaatgt tccccggcct ttggcagact cgataaccga ctctctttta agctcgtcca
     4441 aagcccttcg aagcgtcagc ctgctcacgt tgaattcatt gcagagctcc ttttcggtcg
     4501 gaagtctctt cgaatagact ccctttccta tccgtcgttt aagttcttta tatatccggt
     4561 agtagaccgg agctcccgct tctatcaact cacccctcca agttctttga gagccgaacg
     4621 cacatgaccc ctgttgcgtt tgagagattc ctcacacttt tcctttccga gaccggtaag
     4681 ggccatgaga atcgcaagag gtacctctct gcctgaggct tccagtatct tctccgattc
     4741 ttcgtaagag agatcgctga tctccgagac tattctggtt gccctatcca ccagtttgcg
     4801 attgaggatc ttcaccccta tcatgtagtt tccgaagact ctgccgacct tgaccatcgt
     4861 gacagtgctg atcatgttca agaccatctt ctgagccgtt cctgccttca accttgtgct
     4921 gccggtaatc acttcggggc cggtcctgac ggctatcgta acatcggcaa gctcggtgag
     4981 tcggggagtc ccgacgttgc atatcaagat ggttctggct ccctgctttc tcgcgtactt
     5041 cagtattcct tccacataag gtgtccgtcc cgaagccgag atccccacta ccgcgtcctt
     5101 ttcagttata tctacctttt ttgcttcttc ttcgcctaac tcgatgctgt cctcggcttg
     5161 ttcgactgca tttgcgaggg cttcctggcc cccggctatc atggttacaa acaggtcttt
     5221 cgaaacgcca aaagtgggaa cgacctccgc agcatcaagg acacctagac ggccgcttgt
     5281 gcccgcaccg gcatatacga tccttcctcc catttcaaga gacctgacgc ataactcaac
     5341 cgcagaggca acttcattga gaacctctct aacagacagt ggaactgttg catcctcttc
     5401 gttcataatt ctaagcatct cgtacgtcga tagctcgtct aaattcctcg ttctgggatt
     5461 agatctctcc gtctccagtt gatcaaactt catcctagac ctcctgcggt atctttatcg
     5521 gaagtttccc ttgcgctttg atcgtgccgt ctagaatctt cttaaggctc ctttgcgaaa
     5581 ccgcttcgta gccgtagctg attatgcagt tcttaacgcc ctccaggaaa gcatcatatg
     5641 ggtttctcaa tgctattacc agcgttcttc ccgtcctttg cgaaagcttc aaaagcaact
     5701 ttctctgacc ttcgtgaagg tgagcgttct ccgtaaagac caacattact ccctttcccg
     5761 gggaatcggc agccagtctg tccgcttctt cgacggttat gttaggcgag aagtcaatta
     5821 tcggacagtc tgccatctgc gcgatctctc tggccacagt gggaactccc ttctggggac
     5881 cgtcttcaac ttgaaccaat cttgaaagcc ttacagagaa tattccgctc acactctcga
     5941 ttggaagggg aagaagagaa ctgtcttcca gaatggccgt gatagatctg tcggaaatct
     6001 cctgcatcgc attcagactc tcctgggagg cgatctcttc gatcgagaac ctgtcggcac
     6061 tggtcgcctc tacccggaac ttctcaactc tttgtagaga ttcctggaaa cgcttttcca
     6121 gctctggcga tttttctatc tctctaatca gaatcttctt gactcttctc tggacaaccg
     6181 gatcgtggca gtaagtcaga taatccattc catttctcag accctgtata gcggcttcct
     6241 ctggcgtgaa gtagttgctc actcccccca ttcccagatc atctgcgaca aggaccccgc
     6301 gataattcag cacgttcctg gcaagatccg tcaatatctc tctcgacatc gacgccggaa
     6361 cccgggacgc ctgaagctga ggcatgtata tatgcgaagg cattatcgaa tctgcttgaa
     6421 tttccttgaa tggtctcagt tcgtattcat aaagcaaaga ttgggggatg tccagaatcg
     6481 gtaaatccag atgagcgtcg acatctaccc ttcccttgcc gggaaaatgc ttcaaacagc
     6541 tcatcactct ttcactcttc ataccctcta caaaggccct tgcgtaggct ataacctgat
     6601 ccggagaatc gccgaaactc cttactccga ttcccgggtt tttgggattg cagttaatat
     6661 cgacgacggg agcgagattc caattcacac ctatggcgtt catctctctg gcaagtattc
     6721 ttccaacaac gaaggagttt ttagtgcttc cagtggcggc tattgccata gctccgggcg
     6781 aaacagagaa gccgtccctc agtcggctta ctatcccgcc ttcctgatcg atagcaattg
     6841 cgggcttgta cccaaggact ctttgaatgt catctacgag agcccttacc tgaacagcat
     6901 caacgatatt cttcgaaaag agaataaccg ctccgggttt gacttctcga agtgtccttt
     6961 cggtctcgct atcgatttcc ggaccgggta ttccaatcaa aaacaaccgt cctacggctt
     7021 cgtcgaaatt cattctgaat tctccttggt tttcatacga tacaattcta gtttttatta
     7081 ccttgtattg tcaattgtaa atttgtatag tatgaatata cataacctta gattccgctg
     7141 cttatcgatc atatttgact atagttccat ttaagtaacc gagggaaaac ttggatacag
     7201 atagaaaatg cgatagcatt gctgttggaa gaaccgtggt tgggggtcgg agattggggg
     7261 ttggtggcat caagatcaga agtgatgctg cctccggcag gaagtgaggc
//
LOCUS       DAOQYI010000071         7220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_10739_length_7220_cov_79.614096, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000071 DAOQYI010000000
VERSION     DAOQYI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10739_length_7220_cov_79.614096"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..1113
                     /locus_tag="PKL93_10280"
     CDS             403..1113
                     /locus_tag="PKL93_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNQ71684.1"
                     /translation="MFVLAHRGMGKGRYENTLRSFKEGLSYGAHGVELDVRLTKDGVV
                     ILNHDPDLKRTMGLDVKISEKTFAELEEMGLVGFDALTTLEEIYRELPEDIFVDVEIK
                     AVEAVPEVIKIVKRFDALERTMFSSFHHDCLHEFKEELCCPMVAPIIDKELLRPGGEE
                     FLYKVIERYRPYSVNLNKDLFENIGLEKGLSLLRKIREEGTRVALWTLNDSDLFLKVR
                     DVCDFLITDRSDVMMEVV"
     gene            1110..1634
                     /locus_tag="PKL93_10285"
     CDS             1110..1634
                     /locus_tag="PKL93_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71685.1"
                     /translation="MKFDSRKSLIVVLVNLALLTILAVSSCGEKPNPAEEKLTLSREE
                     ILALCGLEESEISFTKIKLDVSGKPLLTVAAYDGEGRQIFSKDFPQSEVKGGYVTVAV
                     IKSEIIDALRFLYAVTDSKGSVFSFVQDLPYFQYQEVKVEKGDYSHISSKGEFRLLSV
                     TFGQDKQDIFLNVK"
     gene            1659..2327
                     /locus_tag="PKL93_10290"
     CDS             1659..2327
                     /locus_tag="PKL93_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HNQ71686.1"
                     /translation="MKKLILVVIMMASFSTFGASLPKERIQNALGVLQELTQTEDSGS
                     FIELLSKSNGIVIYPEMVKAALGFGGQFGEGFLLKRAEDGRWYGPLFLKLYGISYGFQ
                     AGVQKSGLVFVIMNEKGIEGFLGNNVTLGGSASISAGPTGKTLSADTDYKLEAAIYSY
                     SVSNGIFAGVSLGGSIIRQDTDTNREYYGLSLTPQEIIEKEAIGDDINELINFLESLI
                     VRLN"
     gene            2501..2983
                     /locus_tag="PKL93_10295"
     CDS             2501..2983
                     /locus_tag="PKL93_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71687.1"
                     /translation="MLIVFVVIASVGLSVDIQQIEPLIGSYSEFTSRGYEMEEGSFAT
                     VTAIPFDAISNLDFPIKDVVGLLFFELPEGPSRVSFLNVAVTGKCKRGEPVEGVWADL
                     FIIGEEYIFQTERYNPYIAIDSEGLLIAFCVYLDTSIYYSVTLEGRIMDVQWEFNVGK
                     "
     gene            3028..5835
                     /locus_tag="PKL93_10300"
     CDS             3028..5835
                     /locus_tag="PKL93_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ71688.1"
                     /translation="MRKLSRKISLQFLLFVLAVLTVLVALLSVTFVRTATDEYKSYLS
                     SKKQSVFQWFIDLRKDMKVFVDGYSLEGVIDDGCDILIRLGDERKTILNRPAISEGQL
                     NEIEEAENGFKMLEGQLVYFSTVESNESRYVAGIVFDNEDVEELSAFLGQDGIAFLLI
                     DDHFVIPREFSDSGLYVRTVLDEERWSSEIPFTVSEPRPAFSFFSSGSLFLADKVSIG
                     GADIYVLQSEGLMVMLRNRLLPILSIVLLGVFGFSFVLSFTLNAHISKALSELLKGFE
                     SIKKGSFVRVKLNSNDELGEIASELNNTMVFIEKTLDRLKNSNEMLRKVSKEAQQASK
                     MKSEFLANMSHEMRTPMNAILGFAELLMNDETNLEKMKYLKTIYRSGEHLLSLINDVL
                     DLSKIEAARFDLIISPYSPAKLVKEITETYLPMAYSKGLHFANSITERVPEYVDGDEF
                     RVRQVLTNLVSNGLKFTDKGYVSVLLDYDGHHLIYTVQDTGSGVSREEIDKIFEPFIQ
                     ADGTMSRKFGGTGLGLTITKKIVELMGGSIRFESKVGEGSKVTVRIPSNVSKEVPKQK
                     EKPELPPTGKVVVASDDEDFLTIVGAMLKRNGVYFESVKNLSNLSRAVRELGASLAVV
                     DAPKNADDALAALDGIDEAAFIVITDTTENEIHFGKKVNEIIQKPVKEDELIGKIGNY
                     MDLGPGQDNVKYRILLTEDNEANQLLIKEVLEKAGYSVDVAGNGKQAIEMVRNFNQYN
                     LILMDMQMPIMDGYEATKALRQEGYKIPIVALTAHTMQGDEEKTIEAGCDGFLGKPVR
                     QSDLLEIVRYHLGIFGKTGNKRSGVRPIQREGKHSPDNITVFAKDMGLSMEEASAMFE
                     EYGKHIDKTVATLESALERKDMGSIAREGHSLKGSGRMYGVEEISGVGFKIETAGKSG
                     DENQISDGIAELKRIYRKLWE"
     gene            6154..>7220
                     /locus_tag="PKL93_10305"
     CDS             6154..>7220
                     /locus_tag="PKL93_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ71689.1"
                     /translation="MSKVFFTNMETTPEMGLLKKVEALLKRAGLEDVVQKDKLVAIKL
                     HFGEYGNLAYIRPNYLRVIADQIKKLGAVPFLTDANTLYKGSRSNAVDHITTASLNGF
                     TMESVGVPVVIADGLRGSDEIDVEIEGDYIKKAKIASAIALADSLVVVSHFKGHEQTG
                     FGGALKNIGMGSASRAGKMEQHSESKPVVREKNCVACGMCEKNCPTGAITVDRVARID
                     YEVCIGCGQCIAMCNYGAMSAGAGGTPESLSKKIAEYALAASKGKNALYISFINNVSP
                     DCDCWHINRPPVVEDIGIVAGRDPVAVDKACIDLVIGRLGYDPFERAHPGISWRHQLE
                     HAEKIGLGERSYKLEKVACFG"
BASE COUNT         1953 a         1576 c         1934 g         1757 t
ORIGIN      
        1 atcttgcatc ccctgcctct cttattctaa caaacaacca aggacggttt ttacagcgtt
       61 cagcggttct tatgcttgca actaggaaca gacaacttgc aactgttctc actcgcgcag
      121 tggaactggc cgcttgaagt gcgacggtcc tgatgttctg catcacttgc gtttcttggt
      181 tgttcttgcc gctgcttgca gtgactggcc tcagcttgaa gctgagactg gcgccacgaa
      241 gtggtactgg cggagccttc actggcggtg atgattctcg ccttttgact ggtctgcgaa
      301 agcagactgg ctgttcttcc ctcacattgc cttgactgag tttccataac acagtagaat
      361 gatctcagcg aagtattttg ccgggttgcg gaggcgattt gtatgtttgt tttagcccac
      421 aggggtatgg ggaagggcag atacgagaac acacttagat ctttcaaaga ggggctatcg
      481 tacggcgcac atggagtaga gctcgatgtc aggcttacaa aggatggcgt ggtcatcctg
      541 aatcacgatc ccgatcttaa aaggaccatg ggactggatg taaagatctc ggaaaagacg
      601 ttcgccgaac tcgaagagat ggggcttgtt ggatttgacg ccctgaccac tcttgaagag
      661 atttaccggg aactgcccga agatatattt gtcgatgttg agatcaaagc cgtagaggct
      721 gtacctgaag tgattaagat tgtcaagaga ttcgacgccc tcgaaaggac tatgttttct
      781 tccttccatc atgattgtct tcatgagttc aaagaggagt tatgctgccc tatggtagcc
      841 cctataatag ataaggagct tcttcgaccg ggtggtgaag agttcctgta caaagtgatc
      901 gagagataca ggccttattc ggttaatctc aacaaggatc tcttcgaaaa cataggcctg
      961 gagaaggggc tttcattgct tcgaaagatc cgcgaagaag gaacgagagt tgctttgtgg
     1021 acgttaaacg attcggacct atttctcaag gtcagagatg tgtgcgattt cttgattact
     1081 gaccgctctg atgtaatgat ggaggtagta tgaaatttga cagccgaaag agtctgatag
     1141 tggttcttgt taatttagcc ttgctgacaa ttcttgccgt gtcctcctgt ggcgagaaac
     1201 ccaatccagc agaggaaaaa ctcacacttt ccagagagga gattctcgcc ctttgcggac
     1261 tggaagaaag cgagataagt ttcaccaaga tcaagcttga cgtttctgga aaacccctgt
     1321 tgaccgtggc ggcctacgac ggagagggta gacagatctt ctcgaaagac tttcctcaga
     1381 gcgaagtcaa ggggggatat gtgacggttg ctgtgatcaa atcagagata atcgacgcgt
     1441 tgaggttcct ctatgccgtg accgactcga aaggtagcgt tttctctttc gttcaggatc
     1501 tcccttattt ccagtatcag gaggtaaagg ttgagaaagg cgactattcg cacatctctt
     1561 ccaaagggga attcaggttg ttaagcgtca catttggtca ggacaagcag gatattttcc
     1621 tcaatgtgaa atagaatgaa caacggggag ggattgaaat gaaaaaactg atactggtgg
     1681 tgatcatgat ggcttctttc tcgacatttg gggcaagttt accaaaggaa cggatccaga
     1741 acgccctggg cgtccttcaa gagctcacgc aaacggaaga cagcggttct tttattgagt
     1801 tgttgagcaa gtcaaatggg atagttattt atcccgaaat ggtgaaggct gcgctaggtt
     1861 ttggcgggca gtttggagag ggctttctgc tgaagagagc agaggatgga cgctggtatg
     1921 gccctctttt tctaaagcta tacggtatca gctacggatt tcaggccggt gttcaaaaga
     1981 gcgggcttgt attcgtaata atgaatgaga agggtataga aggtttcctg ggcaacaacg
     2041 ttaccctcgg cggaagcgcc tctatttcgg ccggacctac cggaaagact ctctccgccg
     2101 acaccgatta caaactcgaa gcggctatct actcttactc tgtcagcaac ggtatattcg
     2161 ccggcgtttc cctgggagga tctataatcc ggcaggatac ggatacgaac agggagtact
     2221 acgggctgtc tttgactcct caggagatta tcgagaagga agcaatcgga gacgatataa
     2281 atgaactgat aaactttctc gaatctctta ttgttcggct taactaatcg tgtaactgtt
     2341 acggaaacac catttatcta ttgagatgca actggagctc acggaaagca atctaatctg
     2401 tgagctcctg ttcttatgat atccggccct tgcgtaatat aatctctttg agacaaggtc
     2461 acatttctta acgcgaagga gagattaggt tttgagactc gtgttgattg tctttgtggt
     2521 tatcgcatct gttggtttgt ctgtcgatat ccaacagatc gaaccgctta taggcagcta
     2581 ctccgaattt acttctagag ggtacgaaat ggaagaaggc agtttcgcca ccgtaacagc
     2641 gattccattc gatgcaatat ccaacctcga tttcccgata aaggatgttg tcggtttgtt
     2701 gttcttcgag ctgccggaag gtccctcacg tgtatcgttc ctgaatgtgg ccgttacggg
     2761 gaagtgtaag cgcggagaac ccgttgaagg agtctgggcc gatctcttca taattggtga
     2821 ggagtatatc ttccagacgg agaggtacaa tccttatata gctattgatt cagagggcct
     2881 gttaatcgca ttctgtgtct atctagatac gtccatttac tattccgtaa ctctcgaggg
     2941 tagaatcatg gacgtacaat gggagttcaa cgtcggcaaa tgaaattatg atgcagtctt
     3001 gtccagttga ggaggcagag tcattttatg agaaaactaa gtagaaagat atcgctacag
     3061 tttctgcttt tcgtcctggc agtcttgact gtccttgtgg ctctgctttc cgtcacattt
     3121 gtccgcacag cgaccgacga atacaagagc tatctaagct caaagaagca atccgtcttt
     3181 cagtggttta tcgacttgcg caaggatatg aaggtttttg tcgacggtta ttcgcttgag
     3241 ggagttattg atgatggttg cgatattctc attcgacttg gagacgagcg aaagacaatt
     3301 cttaacaggc cagccatctc agaagggcag ttaaacgaaa tcgaagaagc agaaaacgga
     3361 ttcaaaatgc ttgagggaca gctcgtctac ttctcgactg tagagagcaa cgaatcgcga
     3421 tacgttgccg gaattgtctt cgacaatgaa gatgtggaag aactgtcggc atttcttggg
     3481 caggacggaa tcgccttcct tctgatcgat gatcacttcg tgatcccgag agagttctcg
     3541 gattccggcc tctatgtgag aacggtgcta gatgaggaga ggtggtcaag tgaaatcccc
     3601 ttcaccgtga gcgaaccgag gcccgccttc tctttcttct cctcgggatc gttgttcctc
     3661 gccgacaagg tttcgatcgg cggagccgac atctatgtgt tgcagtctga gggtctaatg
     3721 gtgatgctca ggaataggct actcccaata ctcagcattg tgttactcgg ggtattcggc
     3781 ttttccttcg tgctgtcctt cactctcaac gcccatatat ccaaggccct gtcggagctg
     3841 ctgaagggtt tcgagtcgat aaagaagggc tcctttgtca gggttaaact caattctaat
     3901 gacgagctgg gcgagatcgc ctcagaactg aacaacacga tggtcttcat tgaaaagacg
     3961 cttgacagac tgaagaattc gaatgaaatg ctcaggaagg tttcgaagga ggcacagcag
     4021 gccagcaaga tgaaatcgga gtttctggca aacatgtccc acgagatgcg cacccctatg
     4081 aatgcgattc tcggttttgc cgagctactg atgaacgatg agacgaatct ggaaaaaatg
     4141 aagtatctca agacaatata caggagcggg gaacatcttc tcagtctcat aaacgatgtt
     4201 ctagacctct caaagatcga agcggcgaga ttcgatctga taatctcccc gtacagccct
     4261 gcaaagctgg tgaaagagat caccgaaaca tatctgccca tggcgtattc gaagggattg
     4321 catttcgcaa acagcattac agaaagggtt ccagaatacg tcgatggaga cgaatttagg
     4381 gtgagacagg tcctgaccaa tctggtctct aacggactca agtttactga caaaggttac
     4441 gtttcagtgc ttctcgatta cgatggccac cacctgatat acacggttca ggatacaggt
     4501 tcgggagtct ccagagaaga gatagacaag atcttcgagc cctttattca ggccgacggt
     4561 acgatgtcga ggaagtttgg cggaacggga ctcggtttaa ccataacgaa gaagatcgtt
     4621 gaactgatgg gcgggtcaat acgcttcgag agcaaggttg gcgagggttc gaaagtcaca
     4681 gttaggattc cttctaacgt ttccaaggaa gtaccgaagc aaaaggagaa acccgaattg
     4741 ccgccaacgg gaaaggttgt tgtcgcctcc gacgacgagg acttcctgac aatcgtcggc
     4801 gcgatgctca agagaaatgg agtatacttc gaatctgtga aaaacctttc gaatctctcg
     4861 agagctgtaa gggaattagg tgcgtccctt gcagtggtag acgcgccaaa gaatgccgac
     4921 gatgcccttg ccgcgctaga tgggatagac gaagctgcct tcattgtgat cacagatacc
     4981 accgagaacg aaatccattt cgggaagaaa gtaaacgaaa taattcagaa gcccgtaaag
     5041 gaagacgaac ttatcggcaa gatcggcaac tacatggacc tcggtccggg gcaggacaac
     5101 gtcaaataca ggatattgct aacggaagac aatgaagcca atcagctgct tataaaagaa
     5161 gttcttgaaa aggccggtta ctctgtcgac gttgccggaa atggcaagca ggcgatcgaa
     5221 atggtgagaa acttcaacca gtacaatctc atccttatgg acatgcagat gccgatcatg
     5281 gacggctatg aagctacaaa ggcccttaga caagagggtt acaagatacc aatcgttgcc
     5341 ctgaccgcac atacgatgca gggagacgag gagaagacga tcgaggccgg ttgtgacggc
     5401 tttttgggaa agccggtcag gcagtcggat cttctcgaga tcgtgcgata ccacctgggc
     5461 atattcggga agacgggtaa caagagatcc ggagtccgtc caattcagcg agagggaaag
     5521 cattcgccgg acaatatcac agtcttcgcg aaggatatgg ggctctccat ggaagaggcc
     5581 tcggccatgt tcgaggagta cgggaaacac atcgacaaga cagtggctac tctcgaaagt
     5641 gctcttgaaa ggaaagacat gggttcgatc gcaagagaag gccacagtct taagggttcc
     5701 ggcagaatgt atggggttga agagatttcc ggggtcggct ttaaaatcga gacggccggg
     5761 aagagcggcg atgaaaacca gatatcagac ggaatagcag agctcaagag gatttatcgt
     5821 aagctctggg agtgaggagc ggcgactgca acagaaggtc tctcgcttct acctgggttt
     5881 tcaaggatcg tcaatactct ctgaattttc tcagcagaga tgatcccaga caaatttcta
     5941 gaatgcaatt cacttcgcta taatccggtc tatgaggaac cctgaacctc agccagcaga
     6001 aattctccgt ttagagaagt actggaattt tcgatatgat aacattgaga tcattgtctc
     6061 taacgccgaa aaacctgtcg tgactggaaa gtcctagagc gtaggcattg gacagtccca
     6121 ggcgaaaatc ctttggaaag agaggggaac agtatgagca aggtcttctt tacaaacatg
     6181 gagacgactc ccgaaatggg acttctgaaa aaagtcgagg ccttacttaa gagagccgga
     6241 cttgaagatg tggtccagaa ggataaactt gtcgccatca agcttcactt cggggagtac
     6301 ggaaatctcg catacatccg gccaaactat ctgagggtga tcgccgatca gataaagaaa
     6361 ctgggggccg ttcccttcct cactgatgcg aacactcttt acaaagggag cagatcaaac
     6421 gcagtcgatc atatcacaac cgcctccctg aacggtttca caatggaatc tgtgggcgtt
     6481 cccgtagtga tcgccgacgg cttgagaggt tccgatgaaa tagatgtgga aattgaggga
     6541 gattacataa agaaggctaa gatcgcatcg gccattgctc tcgccgactc gcttgtagta
     6601 gtcagccact tcaaagggca cgaacaaacg ggttttggag gcgctctgaa gaacatcgga
     6661 atgggttcgg cgtcgagggc aggaaagatg gagcaacact cggaatcgaa acccgtagta
     6721 agagagaaga actgtgtagc ctgcggaatg tgtgaaaaga actgccctac tggtgctatt
     6781 actgtcgata gggtcgcaag aatagattat gaggtctgta tcggatgcgg ccagtgtata
     6841 gccatgtgca attatggcgc gatgagtgca ggtgctggag gaacgccgga atctctaagc
     6901 aagaagatcg cagaatacgc tctggcggcc tcaaagggaa agaatgcttt atatatctcc
     6961 ttcataaaca acgtatcgcc agactgcgac tgctggcaca taaacaggcc tccagtcgtg
     7021 gaagacatcg gcatagtggc gggcagagat cccgtagcgg tggacaaggc ttgcatagat
     7081 ctggtgatag gaaggctcgg atacgacccc ttcgaaaggg cccatcccgg aattagctgg
     7141 cgccaccagc ttgagcacgc cgaaaagatc ggcctgggcg aaaggagcta caaactcgag
     7201 aaagtagcct gcttcggcag
//
LOCUS       DAOQYI010000072         7158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_10854_length_7158_cov_72.933549, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000072 DAOQYI010000000
VERSION     DAOQYI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10854_length_7158_cov_72.933549"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1693)
                     /locus_tag="PKL93_10310"
     CDS             complement(29..1693)
                     /locus_tag="PKL93_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71690.1"
                     /translation="MKKKDIVDNNNTLQSTEISKEDLFEVKYMSRWQLVWRALRKHKL
                     GLLSLWILIIMYLIALFADFLAPNSPYEQTQGLSFAPPSKVHWTDEEGKLTAPYIYAW
                     VMERDPETYRTTFAEGVSVGALTAIDKSTGEEITFRKGENGVKDIFLTIKTERYALDA
                     DGNRANIGQPDYSIIQNIKLNELELFERTQVSETTNKTTIDSLSPSRFRELQKIGPPV
                     KVVTERTLHRVFAQKEDEIDDVVLEAISLYNEIIDLDGGDVELAQEFLAELEISPTLL
                     GVIVEPEVKTYETVKYPVKFFVKSWEYKLFWLIPTNIHLFGTDQPSKLLLFGADRYGR
                     DVYSRILAGSRISMSIGLLAIMITFSLGLSIGGAAGYFGGITDEILMRVTEVLMSIPS
                     FYLLISLRAILPTSIPSHLTYLLIVVILSFIGWPGMARVIRGMVLGLKETEFVQAAVA
                     MGYPSSRVILRHIIPNTATYIIVAATLSIPGYILGEAGLSFLGLGITEPSASWGLMLS
                     QAQSIKAMTEAPWLLIPGIFIFIVVMGFNLLGDALRDALDPRSLGY"
     gene            complement(1704..2675)
                     /locus_tag="PKL93_10315"
     CDS             complement(1704..2675)
                     /locus_tag="PKL93_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71691.1"
                     /translation="MLKYIVRRLILAIPVLLGVSILAFMIISAAPGDFLDAYRLNPSI
                     SREQITVLESQFGLDQNVFVQYFKWLGNVLTGNFGYSFSYRIPVFELVWRRLGATLLL
                     SISTLIFTWGIGIPLGIYSALHQYSPSDQAFSFLAFIGISIPNFFFALLWLFMAAKTG
                     WFPIGGIISQNFGDMSFFGKIGDYLWHVAGPMVTLGTSGLAGLMRQMRGQLLDQLRQD
                     YVLFARAKGMPEKNVIYKHALRNAINPIVTMFGYALSGLLGGAVLTETVFGWPGMGRL
                     VIEALNAQDLFLVMATLLLSAVLLVIGNLLADLLLAWVDPRIRYRLS"
     gene            complement(2775..4499)
                     /locus_tag="PKL93_10320"
     CDS             complement(2775..4499)
                     /locus_tag="PKL93_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71692.1"
                     /translation="MKKLSVLLVVLIAAGFLFAATEYHVEETWNGKPGGTFYYWGLGD
                     PKTFNYDWAQETSSTDPLGFILATLIESDEGGMPTLPGLAKKWWFSEDGLTFFVQIRE
                     GLQWSDGEPFTIDDVYWTFVDVSFIPENTANGNGSYLDSNDQLPVVEIVDDTTISFTW
                     TVPMVTALRQIGFRPIMPKHVLEEVVANGTYAEFWTIADFDKLVGMGPFVITDYIEGV
                     RIVFERNPYYWKVDGNGVKLPYFDKLNYELLADQNTSLLRFEAGELDLYGPTAEQFPR
                     LAEMAAEKGWITGVGGPALGSQFVTFNFNATDPVKREWFRNDGFRRAFVYAMDRDAII
                     ESLYNGLGSPLYGPVSPSSGFYNPEIEKFAYKYSITRARLELKRGGFDWLADGTCVDA
                     NGNPVEFELLTNAGNVVREAISNIIVDGAAKLGIKVNFRPIDFNTVVSRLTDATYEAV
                     VIGLTGSVDPGTGWNVYRLDGGLHFWNYPPDYNPDDHITEDIYVLPDWEKRVDEIYRL
                     QTSAVDDQDRYDLFAEFQMIFAEYQPVVFTMAQNFLYVYKNNIRMPVEKLTPATGLLY
                     QLEGWWKE"
     gene            complement(4496..5668)
                     /locus_tag="PKL93_10325"
     CDS             complement(4496..5668)
                     /locus_tag="PKL93_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ71693.1"
                     /translation="MKLLEVRNLKKYFPIKQGFLVERVVGFVKAVDDVSFSVDRGKTI
                     GIVGESGCGKTTIGKSIVRLLEVTDGEMLIDEEDTTFYFMKKRRAKQYLKAKYFDTAR
                     FNNGDNSEFEPYEKKMYDVYNRANRDASKAIDYLFEKADQKKRLLRRKAQIVFQDPMS
                     SLNPRMTVGQMLTEPLLFHELAKDLDEAIEKVKELLVQVGLKPYHVDRYPHQFSGGQR
                     QRIAVARAISVNPDLIVLDEPTSALDVSVQAQIVNLFQKLQEQLNAGYVFISHNLSLV
                     RFISQEVSVMYLGRIVEQGNSESIFKNPLHPYTKALLAAAPIPDPKKKRNRKDLVGGQ
                     VPSPINRPTGCFFNPRCKYRMDICTKEYPPMVRVDENHFVACHLYSTSQEQGGESK"
     gene            complement(5665..6702)
                     /locus_tag="PKL93_10330"
     CDS             complement(5665..6702)
                     /locus_tag="PKL93_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71694.1"
                     /translation="MQERPILSVKNLSTHFNMAEGVVKAVQDVSFDLHRDDVLGIVGE
                     TGSGKSVTVKSIAGMTDSPGFIAGGEILFFTDEFSKNGEKEYIDLVKLPKESFSKVRG
                     KHIGMIFQDPMTSLDPMYTIGNQMIETIVHHKKVTEEEARERSIRLLEQVGIPKPSER
                     INDYPFQLSGGQRQRVVIAIALSCDPEILVADEPSTALDVTVQAQILELMKDLQEQFN
                     SSMIFITHDLAVIAEIATKISVMYGSYQMEMADSMEIFENPMNPYTFALLECIPRLDI
                     KQDQLLPIPGQPPVMLNPPNLCPFLPRCSRATDRCYKELPKLEQLKDNHFVRCWNPIT
                     NRVLLVKEAEE"
     gene            6813..>7158
                     /locus_tag="PKL93_10335"
     CDS             6813..>7158
                     /locus_tag="PKL93_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNQ71695.1"
                     /translation="MLEAVREIWFVEGTTYIKICYGNVFVDGEAGQLGDRGEVDSFKI
                     KSVKKIEDEVVLEIDGKIEKNKGEMVDIRIDEERRLDISQQHTAQHLLSAVFLSEMDA
                     ETVGFQMGEEYTT"
BASE COUNT         1971 a         1677 c         1523 g         1987 t
ORIGIN      
        1 atacaatcaa tctattaggt tcttaggttt agtaccctag agatcttgga tcgagagcat
       61 ctctcaaggc gtcgcctaga aggttgaatc ccatgaccac aatgaatata aagataccag
      121 gaataagaag ccaaggagct tcagtcattg ctttaatact ctgtgcctgt gaaagcatca
      181 acccccagct tgcagaaggt tctgtgattc cgaggcccag gaagcttaac ccggcctcgc
      241 ccagtatata gcctggtata gacagagtag cggctacaat aatgtaggtt gccgtgtttg
      301 gaataatatg ccttagtatc actcgagaag aaggatagcc catagcaaca gcagcctgga
      361 cgaattccgt ctcctttaga ccgagaacca ttccacgaat gacccttgcc attccaggcc
      421 agccgatgaa gctaagtatt accactatca gcagataggt caaatgtgat ggaatgctgg
      481 tgggaagtat tgcccgcaat gagatcagca agtagaaact cgggatagac atcaaaactt
      541 ctgttactct cattagtatc tcgtcagtaa ttccaccaaa gtaaccggca gcacctccta
      601 ttgataggcc aagagaaaaa gttatcatta tagcaagtaa accaatactc atggagattc
      661 ttgagcctgc cagaattctt gaatagacat ctcttccgta tctgtctgct ccgaataaaa
      721 gcagcttcga aggctggtcg gtaccgaata gatgaatgtt tgttgggatc aaccagaaga
      781 gcttgtattc ccaagacttc acaaagaatt taacagggta cttcacggtt tcgtaagtct
      841 tgacttcagg ctcaacgatt accccaagaa gagtaggaga gatttcaagc tcggcaagaa
      901 actcttgagc aagctccaca tctcctccgt caagatcaat tatttcgttg taaagactta
      961 tggcttcaag aacaacatcg tcaatttcat cctctttctg agcgaagact ctgtgaagtg
     1021 tcctttcagt caccaccttc acaggtggtc caattttttg aagctctcta aacctgctag
     1081 ggctcaaaga gtctattgta gttttgttcg tggtttcgct aacctgtgtc ctttcaaaaa
     1141 gctccagttc attcagtttg atgttctgga taatggaata atctggttgc cctatgttgg
     1201 ctcgatttcc atctgcgtct aaagcgtatc tttcggtctt tattgtgaga aagatatctt
     1261 ttactccgtt ctcacccttc ctaaatgtga tttcctcgcc tgtggacttg tcgattgccg
     1321 tcagcgctcc tacggaaaca ccttcagcaa aagtcgtcct gtatgtttct ggatcccgtt
     1381 ccatgaccca ggcataaatg taaggagcag tgagcttccc ttcttcatct gtccagtgaa
     1441 ctttagatgg cggggcaaac gatagaccct gagtctgctc gtaagggctg tttggcgcaa
     1501 ggaaatctgc gaataacgca attaggtaca ttataatcag tatccagagg gaaagcaaac
     1561 ccaacttgtg ctttctcaag gctctccaaa cgagctgcca gcggctcata tattttactt
     1621 caaagagatc ctctttgctg atctctgtgc tttgcaatgt attattattg tcgacaatgt
     1681 ctttcttctt catttcacac ccctcagctt agcctataac gtattctggg atcgacccag
     1741 gcgagcaaca gatcagcaag caaattgccg attaccagaa gtacagcaga aaggagtaat
     1801 gttgccataa caaggaacaa atcctgagcg tttaaggctt ctataacaag tcttcccatt
     1861 cccggccaac caaaaaccgt ctccgtcagg acagcccccc caagcaagcc ggaaagtgca
     1921 tatccaaaca tagtaacgat aggatttatt gcatttctta atgcgtgttt atatatgaca
     1981 ttcttctcgg gcattccctt tgctcttgcg aagagaacat aatcctgcct gagttgatca
     2041 agaagctgac ctctcatctg tctcatcaga ccggccaacc cggatgtgcc aagagtgacc
     2101 atcggacctg caacatgcca aaggtagtct ccaatctttc caaagaaact catgtcgccg
     2161 aagttctgag atattatccc acctattggg aaccaaccgg tttttgccgc cataaaaagc
     2221 cacagcagtg caaagaagaa gtttggaatt gagatgccaa taaacgcaag aaaggaaaag
     2281 gcctgatcgc tgggagagta ctgatgaagc gcagaataaa tacccagagg aataccaatt
     2341 ccccacgtga aaatcagagt gctgatgctc aacaaaagag ttgccccaag tcttctccac
     2401 accagttcga aaactggaat tctatagctg aaagagtacc cgaaattacc tgtaagaaca
     2461 ttacccaacc atttgaaata ctgtacaaat acattctgat caagcccaaa ctgactttcg
     2521 agaacggtga tttgctccct cgaaatggaa gggttcaatc tgtatgcatc caaaaaatct
     2581 ccaggggcag cggatataat catgaaggcc agtatcgaga ccccaagcaa gacgggaatt
     2641 gcaagaatca gccttctaac tatatatttc agcacctcat tccctcctca atactttagc
     2701 tgttttgcga atgatttcga ctaatattag taaaagcggg aggaaagccc tcccgcttaa
     2761 tgaaacgcgc taaattactc tttccaccaa ccttcaagct ggtaaagaag accagtggca
     2821 ggtgtaagct tttccaccgg catccttatg ttgttcttgt aaacataaag gaaattctga
     2881 gccattgtaa agaccactgg ctgatactct gcaaaaatca tctggaactc cgcaaacaaa
     2941 tcatatctgt cctgatcgtc aactgcggac gtctggagtc tgtagatctc gtctactctc
     3001 ttctcccagt ccggaagaac gtaaatgtct tccgttatgt ggtcatcagg attgtaatcc
     3061 ggagggtagt tccagaagtg caaaccgcca tcgagcctgt aaacgttcca acctgttccg
     3121 gggtctacac tgccggttaa accgattact acagcttcat aggttgcatc agtcagtctt
     3181 gaaacaacgg tattgaagtc aatgggtcta aaattgacct tgattccgag cttcgcagca
     3241 ccatctacta tgatgttgct aatcgcttct cttacaacgt ttccagcgtt cgtaagaagt
     3301 tcaaattcaa ctgggtttcc atttgcgtcg acacaggtgc cgtctgcgag ccagtcgaag
     3361 ccgcctctct ttaattcaag tctagccctt gtgattgaat acttgtaagc gaacttctcg
     3421 atttcgggat tgtagaagcc gctggacggt gatacgggtc cgtacagtgg agaaccgagt
     3481 ccgttgtata gggattcaat gatggcatct ctgtccattg catatacaaa ggcccttctg
     3541 aatccgtcgt ttctgaacca ttctctcttg accggatctg tggcgttgaa attaaaggtc
     3601 acgaattggg atccaagagc agggccacca acacccgtta tccaaccctt ttctgcggcc
     3661 atttctgcta gccttgggaa ctgctcagca gttggtccgt aaaggtcaag ttcgcctgct
     3721 tcgaatctaa gaagagaagt gttctggtct gcaagaagct cataatttag cttgtcgaag
     3781 tagggcagct taactccgtt gccatcgact ttccagtaat atgggtttct ttcgaagact
     3841 attcttacac cttcgatata gtctgttatt acgaatggtc ccattccaac aagtttgtca
     3901 aagtctgcaa ttgtccagaa ttctgcgtaa gtcccgttgg caacgacttc ctcgagaacg
     3961 tgctttggca ttatcgggcg gaagccaatc tgcctcaatg cggtaaccat agggacagtc
     4021 catgtgaaag aaatcgttgt gtcatcgacg atctcaacca ctggcagctg atcgttcgag
     4081 tctaggtaag agccgttccc gtttgccgtg ttctcgggaa tgaaagacac atcgacaaat
     4141 gtccagtaga cgtcatcgat agtaaaaggc tcaccgtcag accactggag accttctctg
     4201 atctgaacga agaatgtcag tccgtcttca gagaaccacc attttttggc caagccagga
     4261 agtgtaggca tgccgccctc atcggattcg attagagttg ccagtatgaa tccaagagga
     4321 tcggttgaac tggtttcctg tgcccagtca tagttaaatg tctttggatc tcccaagccc
     4381 caataataga aagtgcctcc gggcttgccg ttccatgtct cttctacgtg gtactcagta
     4441 gcggcaaaaa gaaatcctgc tgcaatgaga accactagaa gcactgaaag tttcttcatt
     4501 tactttcccc tccttgttct tgagatgtgg aataaagatg gcacgcaaca aaatgattct
     4561 catccaccct gaccatgggt ggatactcct tagtacaaat atccatcctg tatttgcatc
     4621 ttggattgaa gaaacaaccc gtaggtctgt ttatcggact cggtacctgt ccgccgacaa
     4681 ggtctttacg atttcgtttc ttctttggat ctggtatagg agcggcggca agaagagcct
     4741 tggtataggg atgcaacgga ttcttaaaga tcgactcact attaccctgc tctacgatcc
     4801 ttccgagata catgacagat acttcttgag atatgaacct cactaaagaa aggttgtgag
     4861 aaatgaaaac ataacctgcg ttaagctgtt cctgaagttt ctggaacaga tttacaatct
     4921 gtgcctgaac ggatacgtct aatgcggaag tcggctcatc gaggacaatt agatcggggt
     4981 tgacgcttat cgcccttgct acggcaattc tttggcgttg gccaccactg aactgatgag
     5041 gatatctatc gacgtgatat ggtttcaggc ccacctgaac gagcaattcc tttacctttt
     5101 cgatggcttc atcaagatcc tttgcaagtt cgtgaaagag aagcggttcg gtgagcatct
     5161 gtcctaccgt cattcttgga ttcaaagagg acattggatc ttgaaagact atctgcgcct
     5221 ttctcctaag gagtctcttc ttttgatctg ccttttcaaa gagataatca atggccttcg
     5281 atgcatctct gttcgctcta ttataaacat cgtacatctt cttctcgtat ggttcaaatt
     5341 ctgaattatc accattgttg aatctcgcag tatcaaagta tttcgcctta aggtattgct
     5401 ttgcacgacg cttcttcatg aaataaaaag ttgtgtcttc ttcgtcaatg agcatttccc
     5461 cgtcggttac ttcaagcagg cggactatcg atttccctat tgtcgtcttg ccacagccgg
     5521 attctccaac gataccaatg gtcttgccac gatcgacgga aaacgataca tcatcaactg
     5581 cctttacgaa accaaccact ctttcaacca ggaaaccttg ctttattgga aagtacttct
     5641 tcagatttct aacttcaaga agcttcattc ttcagcctcc ttgaccagca acactctatt
     5701 ggtgattggg ttccagcacc tgacaaaatg attatcctta agttgttcaa gctttggtaa
     5761 ttccttatag caccgatcag ttgcccttga acaccttgga aggaagggac aaaggtttgg
     5821 aggattaagc attactggcg gctggccagg aatcggaaga agctgatctt gctttatatc
     5881 aagtctaggt atgcattcaa gaagagcaaa agtgtagggg ttcattggat tttcaaaaat
     5941 ctccattgaa tctgccattt ccatttgata gcttccatac atgacactga tcttagtagc
     6001 tatttcggcg atcaccgcca aatcgtgcgt gataaaaatc atgctactat tgaactgctc
     6061 ttgaaggtcc ttcatcagtt caagaatctg agcctggacc gtaacatcca gagcagttga
     6121 aggttcgtcg gctaccagaa tctctggatc gcacgaaagc gctattgcta ttactactct
     6181 ttgacgctgg cctccggaca actgaaaggg gtagtcatta atcctctccg aaggtttggg
     6241 aattcctact tgctcaagca atcttattga gcgttccctt gcttcctctt cggtaacctt
     6301 tttgtgatgg acaatagttt ctatcatctg gttcccaatt gtgtacatcg ggtcaagaga
     6361 agtcatgggg tcttgaaaaa tcattccgat gtgttttccc cttaccttgg aaaagctttc
     6421 tttagggagc ttgaccagat caatatactc cttttctcca ttcttgctga actcatccgt
     6481 gaaaaataga atttcacccc cggctataaa accgggactg tcagtcatcc cagcaatgga
     6541 cttgactgta acacttttcc ccgagccagt ctccccaact attcccaaga catcgtccct
     6601 atgaagatcg aaagacacat cttgaacggc cttaacaact ccctcggcca tgttgaagtg
     6661 agtagacaga ttctttaccg aaaggattgg cctttcctgc aaccaaatca cctcatattc
     6721 atttttcgcg ttatcaccca cggagttaat gaatgctact gtataattat tacatatatt
     6781 tgcacataat atcaagtggc cgggggcggt gtatgttaga agcagtaagg gaaatatggt
     6841 ttgtagaagg tactacctac atcaaaatat gttacggaaa tgtttttgtt gatggtgaag
     6901 cgggacaact tggagaccgt ggagaggtag attctttcaa aataaaatct gtgaagaaaa
     6961 ttgaagacga ggttgtctta gaaatcgatg gaaagattga aaagaataag ggcgaaatgg
     7021 ttgatattag gatcgatgag gaaagaagat tagatatctc acagcaacac acagctcaac
     7081 atcttctttc tgctgtcttt ctaagcgaaa tggatgccga aactgtagga tttcagatgg
     7141 gcgaggagta cacgacta
//
LOCUS       DAOQYI010000073         6966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_11224_length_6966_cov_85.031399, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000073 DAOQYI010000000
VERSION     DAOQYI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11224_length_6966_cov_85.031399"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKL93_10340"
     CDS             <1..186
                     /locus_tag="PKL93_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNQ71696.1"
                     /translation="EIGFELGMSLIRFVRAKGVKVALWTLNDPALLIKIRGEVDIIIT
                     DNLRGIMAALGGKGERR"
     gene            183..950
                     /locus_tag="PKL93_10345"
     CDS             183..950
                     /locus_tag="PKL93_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNQ71697.1"
                     /translation="MTALLYLEKSASAILTPPGLFILIFLIIAVILIKKSEERRTKIS
                     GVVMIILCFFAYLLSTGFGTHLYLQPLERAYTAPNNVSGQAIVVLSSGIIISPEGQVL
                     DNHSVARLSKAYSLHLQTKLPIIVTGSFMPGRSTVAVSELMRDWLLDRGVASSKVIVE
                     PKAQTTWENARYSADICSQFNWSSIVLVTSAVHMKRAVTSFEKFGLRVTPFPTDYLYD
                     HTKLSFVDFLPNRGALDANLASIHEYFGQLWYLFKSP"
     gene            963..2255
                     /locus_tag="PKL93_10350"
     CDS             963..2255
                     /locus_tag="PKL93_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HNQ71698.1"
                     /translation="MCDTFVVLGSSTPDGITLFGKNSDREPNEPQAVYFFPRSSNNPD
                     ELFTTNQKVDQVSETNAILISKPSWMWGGEMGVNDKGVVIGNEAVFTKERVRRDGLLG
                     MDILRIALERSENAEHAVRIIIGILEKYGQGSNGGFTKTLYYHNSFLVADRENAWIVE
                     TSDRFWVTKRVYGSAAISNCLTIETEIDEMHPQVVSNSQDRKWHNPAKEFDFAGSYER
                     RLFRKFSGAEIRLKRMRELIEEKEMNTERCFEILRDHNDRRPQSSMRNICMHAGAGVV
                     TSQTTASMVVAIGDKIEVWVTNSSLPCLSIYKPVWFNSQESSLSFGEGESGLNYWGNW
                     EIFNRLAILRDSKAAELWKEYCLPFEQDLLLSRGKTTEADLTKQAFEKSWKIARKMTS
                     ILRGEREEAGFLKKSYWKKQNAMLQRLKSRNFRKELPT"
     gene            complement(2248..>2625)
                     /locus_tag="PKL93_10355"
     CDS             complement(2248..>2625)
                     /locus_tag="PKL93_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ71699.1"
                     /translation="MDKPMLLDAEKSFLNLFGKNTLLLGGKKVLSTEVLRDRWKCLHT
                     PGHTEGSCCFLFDERFLFSGDTVFSTSVGRTDLPGGDHDQMKSSIDRLKELFTKMPDL
                     IVFPGHGPQTTARIILRENPFFM"
     assembly_gap    2626..2725
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2844..4517
                     /locus_tag="PKL93_10360"
     CDS             2844..4517
                     /locus_tag="PKL93_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ71700.1"
                     /translation="MKNSRICGLALRFNYDNPEDFRSELRSIYEQEDLSREEIGMLLA
                     LDFAYSSTFDEAREFDSSDTDLKLLAEGVYSLSYFYARNSEDKIAYLVSKLAVDHIAR
                     ECEREQEINLKILHMMTSFEMGFGEETLLLFEELVKLEKYVPIQKRFEYYNDLGLVST
                     QLQTGGNPLRFYEKAKELAGSPVRTLMVQLNMIDYLYSKNMFKEALKLLEKTESCNVV
                     SINGYKLMINLKILLQMSNLARAAAVADQLESLIRSYGEWTDIAVSYIFLGHFYVKIG
                     NISKAQNYLNLLKSLPDESLTNYIRGETLILEASIMQVKGEHFRALENSVGAFETLTL
                     YSTTSPHLKDFVNNLFGSISAVFTQLIRELRLKDSYTALHTLRVLKICYEFGKELGLE
                     KIDLFNLSIGAMLHDYGKVDIPFDLLNKPSTLTEEEFTEIMNHPVYGERYLRDLNFPK
                     AIRDIVRHHHERIDGNGYPDGLKGDQIHKLVQIVAVADVFDALTTDRPYRKAITKEKA
                     LEYLKEKGDQLISKGLLSRFIRFAQHKEIIIQEKEITALWRSIISELFK"
     gene            complement(4542..4688)
                     /locus_tag="PKL93_10365"
     CDS             complement(4542..4688)
                     /locus_tag="PKL93_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71701.1"
                     /translation="MKKMIAILLIGLVSFGTVALAGPGLWKIPEGEFTVDGGPGLWKI
                     PEGY"
     assembly_gap    4783..4882
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4887..5033)
                     /locus_tag="PKL93_10370"
     CDS             complement(4887..5033)
                     /locus_tag="PKL93_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71702.1"
                     /translation="MKKVAAILLIGLIVLGGVALAGPGLWKIPEGEFTVDGGPGLWKI
                     PEGY"
     gene            complement(5609..6142)
                     /locus_tag="PKL93_10375"
     CDS             complement(5609..6142)
                     /locus_tag="PKL93_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNQ71703.1"
                     /translation="MILLNLFWSFFKIGFLAFGGGYGALSLIQDQIVNVNKWIGIEEF
                     LTLISISQMTPGPIAINSATFIGYRIAGIQGSILATVGVVLPSVFWIFLILKILKILS
                     KWIDTAEVFNALRLGIIALILSATLRIGIESINSVFTIVTAVSAFYILYKFKLSVIWI
                     VFGTGILGVFWSFFLPL"
     gene            complement(6139..6672)
                     /locus_tag="PKL93_10380"
     CDS             complement(6139..6672)
                     /locus_tag="PKL93_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNQ71704.1"
                     /translation="MSPLRLFWIFVKVSTFTIGGGYAMIPVIKEFIVDKYRIMKEDEF
                     LDVIVTAQTVPGVIAINTAMILGSRLAGFWGAFFAVLGASLTPFIMILVIATFFTDFV
                     DLPVFEGFFAGARVGVTVILANLSLQLLRKSLKRYLIIIVIAAGTLVIVFLNVSSIWV
                     LLVCSLLIYFIDRRSTR"
BASE COUNT         2124 a         1397 c         1457 g         1788 t
ORIGIN      
        1 gagatcggct tcgagttggg aatgagtttg attagatttg taagagccaa aggagttaag
       61 gttgcactct ggacattaaa cgatccagca ttgctcatta agatcagggg tgaagtagac
      121 attataatta ctgacaatct tcggggtata atggctgcac ttggaggaaa aggcgaaaga
      181 aggtgacggc attgctgtat cttgagaaat ccgcttcagc tattcttaca ccaccgggac
      241 tattcatcct aatctttctt ataattgcag tgattcttat taagaaaagc gaagaacgac
      301 gcacaaagat ctctggagta gtcatgatta ttctgtgttt cttcgcatat cttctctcta
      361 cgggctttgg aactcaccta tatcttcagc ctttggaaag ggcatatact gcgccaaata
      421 acgtgagcgg tcaggccata gtggtcttga gcagcgggat aattatttct cctgaaggtc
      481 aggtactcga caatcactct gtcgctagat tatctaaagc ctactctctt catctacaga
      541 cgaagctgcc cataattgtg acaggatcgt ttatgcctgg cagatccacg gtggccgttt
      601 cagaacttat gagagactgg cttttggatc gtggtgttgc atcatccaaa gttatcgttg
      661 aaccgaaggc tcagacaacc tgggagaacg caaggtattc tgccgacata tgcagtcagt
      721 tcaactggtc cagcatagtg cttgttactt cggcggttca tatgaagaga gcggtcactt
      781 catttgagaa gttcggtttg agagtaacac cctttcctac agactattta tacgatcaca
      841 caaaacttag ttttgttgat ttccttccta atcggggagc tttagatgct aatctggcgt
      901 ccattcatga gtattttggg cagttgtggt atctttttaa gagcccttaa gaagctgaga
      961 ttatgtgcga tacttttgta gtacttggaa gttcaactcc ggacggtatt accctgtttg
     1021 gaaagaacag cgatcgagaa ccaaacgaac ctcaggccgt atactttttt cctcgctcat
     1081 caaacaatcc cgacgaactc ttcacgacaa accaaaaagt ggatcaggta agtgagacca
     1141 acgcaattct catttctaaa ccttcatgga tgtggggcgg cgaaatggga gtaaatgaca
     1201 agggagtagt tatcggtaat gaagcagtct tcaccaaaga gagagtgcga agagatggtc
     1261 ttcttggaat ggacatactg aggattgcgc ttgagagatc tgaaaatgca gagcacgctg
     1321 taagaataat aatcggaatt cttgagaaat acggtcaggg aagcaacggg ggtttcacga
     1381 aaacgctcta ttatcacaat tcatttctgg ttgcagatcg agagaatgcg tggattgttg
     1441 agacttccga tcgattttgg gtaaccaaaa gggtttatgg atcagcggcg atttcgaatt
     1501 gcctcactat tgaaacggag atcgacgaaa tgcatcctca ggttgtcagt aattcacaag
     1561 atcgaaaatg gcataatcca gctaaggaat tcgattttgc cggttcatat gaaaggagat
     1621 tattcaggaa gttctcgggt gccgaaatca ggctgaaaag aatgagagag ctaattgaag
     1681 agaaagaaat gaataccgaa agatgtttcg agatccttcg agatcacaat gacagaagac
     1741 ctcagagctc aatgagaaat atttgcatgc atgccggggc aggagttgta acttctcaaa
     1801 caaccgcttc catggttgta gctattggag ataagattga ggtttgggtc acaaatagca
     1861 gtctgccgtg cctttcaata tataagccag tttggttcaa cagtcaagaa tcaagcttat
     1921 catttggaga aggggaaagc ggcttgaact actggggaaa ctgggagatc ttcaaccgac
     1981 ttgccatatt gagagattct aaagccgcgg agttatggaa agaatactgc ctcccttttg
     2041 aacaggactt gttgttaagc agggggaaaa caactgaggc agatctaacg aaacaggcct
     2101 ttgaaaaaag ctggaagatt gcaaggaaga tgacctctat tcttagagga gaaagggaag
     2161 aggcaggttt cttaaagaaa agctactgga agaagcaaaa cgcaatgctt caaaggctga
     2221 agtccagaaa cttcaggaaa gaattgccta catgaagaac ggattttctc tcaagataat
     2281 tctagcagtt gtctgcgggc catgtcccgg gaaaactatt aaatcaggca ttttagtgaa
     2341 aagttctttg agtcgatcta tgctgctctt catttgatca tggtcgcctc caggcagatc
     2401 tgttcttccg acagaagtag aaaacacggt gtctcctgag aacaaaaatc tttcgtcgaa
     2461 aagaaagcag caagagcctt ctgtgtggcc tggagtatga aggcatttcc aacgatcacg
     2521 aagtacttca gtagataaca ccttctttcc tccaaggagg agagtattct tcccgaataa
     2581 attgagaaag cttttttcgg catccagtaa cattggcttg tccatnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnttcgg agtgaaaata cacaactctt ttgaaagctc
     2761 ccgatggctc aaagcaccac actttcgatt ttaccagata gccttcgcca tgatagaata
     2821 atggggggga agaggagttg gatttgaaga attctagaat ttgcggtctg gccttaagat
     2881 tcaattatga caatcccgag gatttcagat cagagctgag atccatttat gagcaagaag
     2941 acctttctag agaagagatt ggaatgcttc tggctctcga ttttgcttac tctagcacgt
     3001 ttgatgaggc gagggaattt gattcctctg atacagatct caagctcctg gccgaaggag
     3061 tgtattctct gtcctatttt tatgccagga attccgaaga caagatagcg tacctcgtat
     3121 cgaagcttgc ggtagatcac attgccagag aatgcgaaag ggagcaagag attaatctga
     3181 agatactcca tatgatgacc tcgttcgaaa tgggcttcgg tgaagaaacg ctacttctct
     3241 tcgaagaact agtaaagctt gaaaagtatg ttccaattca gaagcgcttt gagtattaca
     3301 acgatcttgg tcttgtatca actcagctcc aaactggcgg caatccgttg aggttctacg
     3361 agaaggctaa ggaacttgcc ggttcgccgg taagaactct gatggtacag ctgaacatga
     3421 ttgattatct ttactctaag aacatgttca aagaagctct taagcttctt gaaaagacgg
     3481 aaagctgcaa cgtagtttcc ataaacggat acaaactcat gataaatctc aagatcctgc
     3541 tgcaaatgag taacctcgca agagcagctg ccgttgccga tcaacttgaa tccctaattc
     3601 gttcttatgg ggaatggacg gatatcgcag tatcttacat ttttctggga catttctacg
     3661 tgaagattgg caacatctct aaggcacaaa actatctaaa tcttctgaaa tctcttccgg
     3721 acgaatccct tacaaactat atcagagggg agacgcttat tctcgaggcg tcgataatgc
     3781 aagtcaaggg agaacatttt cgagcacttg aaaactctgt tggcgctttt gagacgctta
     3841 cactttacag tacaacgtcg ccacatctta aagatttcgt aaataatctc tttggaagta
     3901 tttcggcagt tttcactcag ctcatcagag agctcaggct caaggatagc tataccgctc
     3961 ttcatacact tagagttctg aagatatgct atgagtttgg taaagagctt ggactagaaa
     4021 agatagatct gttcaatcta tcaattggtg caatgttgca tgattacggc aaggtcgaca
     4081 taccattcga cttgttgaat aagccatcaa cgttgactga agaagaattc acggagataa
     4141 tgaatcatcc cgtatacggc gaacgttatt tgcgggattt gaactttcct aaagcaataa
     4201 gagatatagt cagacatcat cacgaaagaa tagacggaaa cgggtacccc gacggtttaa
     4261 agggagacca aattcacaag ctcgttcaaa tagttgcagt agcagacgtg ttcgatgcac
     4321 tgacgaccga tagaccatac agaaaagcga ttacaaagga gaaagctctg gagtacctaa
     4381 aagaaaaagg cgaccaatta atctcaaaag gtctcctctc taggtttata agatttgctc
     4441 agcacaaaga aataatcata caagaaaaag aaataactgc actatggcgt tcaataatct
     4501 ccgaactctt caaataagac gaaagagctg cggctaaaaa atcaataacc ttcgggaatc
     4561 ttccaaagtc ctgggccacc atcaacggta aactcgcctt cgggaatctt ccaaagtcca
     4621 ggtcctgcaa gcgcaaccgt accaaacgat accaaaccta tcaaaagaat tgcgatcatc
     4681 tttttcatca aactaacctc cctttatccg tgaaaaaatc gatgttgaaa atcaataacc
     4741 ttcgggaatc ttccaaagtc ctgggccacc atcaacggta aannnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnaaaatcaa taaccttcgg gaatcttcca aagtcctggg
     4921 ccaccatcaa cggtaaactc gccttcggga atcttccaaa gtccaggtcc tgcaagcgca
     4981 actccaccaa gtactatcaa tccaattaga agaattgctg ctaccttttt cattcactcc
     5041 acctccaaaa atctgcttga tgaatttcgt tctgtttcag caccttactc ttagtgatct
     5101 tcttcttcag gtatcttcca catataccac acctcccggt caagtgttcg agtgataaat
     5161 taccttttga atctctctcc acctctaaaa tctcttatca aaactcaagc tcctacgaat
     5221 aaatgtttgg tcgggtggac aataaaaaac aacagtacct ctacataaac aagattacaa
     5281 ctagattcta gatcaagagc attcaaatac gtaaacatca atagaagaag aatgagagca
     5341 gtaatcaggt ttgactggtg ataaccgctt ataaacgact atgatgaagt gtagagcgca
     5401 taatcaaacg taagaaatat aaatatgaat aaatcatatg atgtactatt tattataacc
     5461 tttgcgaggc agcactagaa acttatctca caaaaattaa actaataatt catgcgagtg
     5521 caaaaatcaa aaagtgaaat cgaaagttcg cattgtctaa tgaggcgaat accttttata
     5581 agtttggtat ttgctagttt gttgggattt atagaggtaa aaagaagctc caaaaaacgc
     5641 ccaatatgcc cgtaccaaat acgatccaga tgactgaaag tttgaatttg tagaggatgt
     5701 agaaggcaga tactgctgta acaatggtga aaacactatt aatagactca atccctatcc
     5761 ttaaagtagc cgaaaggatc agtgcgatta tcccgagcct tagggcatta aagacctctg
     5821 ccgtgtcgat ccatttcgat agaattttca gaatctttag tatcagaaaa atccagaaca
     5881 cgcttggaag aacaactccc acagttgcga gaatcgatcc ttgtattccg gcaattctgt
     5941 agccaataaa agtggccgag ttgatcgcaa tcgggccggg agtcatctgt gagatggata
     6001 ttagagtaag gaactcttca attccaatcc atttgtttac gtttactatt tggtcctgga
     6061 ttagacttag agcgccatat cctcctccga aagctagaaa accgatctta aagaaagacc
     6121 agaagagatt gagtagaatc atctggtcga cctcctgtcg atgaaataaa tgagaagcga
     6181 gcataccagc aaaacccaga tagacgatac attaagaaag acaattacca aagtaccagc
     6241 agcgataacg attataatca ggtatctttt aaggcttttt cgaagaagct gaagggaaag
     6301 attggcaagt attacagtta cacccactct cgcgccggca aagaagcctt cgaagacagg
     6361 aaggtctaca aaatcggtga agaaagttgc tattacaagt atcataataa atggagtcaa
     6421 cgaagctcca agcaccgcaa aaaaagcgcc ccaaaaccct gcaagcctac ttccaaggat
     6481 catagctgtg tttatcgcta taacaccagg tacagtttgg gcagtgacaa tcacatcgag
     6541 aaactcgtct tccttcatta tcctgtactt gtctacaata aactccttta ttacaggaat
     6601 cattgcatat ccgccgccta tagtgaatgt actgactttg acgaatatcc agaaaagcct
     6661 aagtgggctc atgatttcaa cgtccatcta atccatgctg gagcgacgaa cttcctcctc
     6721 tcaccattat gagtgtacag tcaaatgcag taacctggaa agactctaag tcttctgttc
     6781 tccgcccatt tgctcctata ttttacaact cgttctggtt ttctgcagtc ataagtcaag
     6841 ttatcctttt gaatggagca tgtcttacgt gataaacttt actaaagggg gtgaacctat
     6901 ggaaaacaga atcagatggg gcgccaaagc gatttcttcc gacggcaaag acctcggaaa
     6961 agtcat
//
LOCUS       DAOQYI010000074         6936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_11289_length_6936_cov_53.268420, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000074 DAOQYI010000000
VERSION     DAOQYI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11289_length_6936_cov_53.268420"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            731..1129
                     /locus_tag="PKL93_10385"
     CDS             731..1129
                     /locus_tag="PKL93_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71705.1"
                     /translation="MKRVAFMVLVAIAVVILLSACPSVAKTRSLVGTWNLSVIKSESE
                     TFTAFMQITTHEKGNLSGYLGNHESYGNYGTISGTTSKDKIIFLYSSDEGGETIQFTG
                     KFDSNGMTGTATVYINGSYKSGPHNWTAHR"
     gene            complement(2588..3604)
                     /locus_tag="PKL93_10390"
     CDS             complement(2588..3604)
                     /locus_tag="PKL93_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ71706.1"
                     /translation="MILVTGSTGHVGNVLVRELVSRGESVRAVALPGEDTSMIELEGV
                     TVLRGDVRDRDFVIEACEGIDTVYHLAALISIMPTDKKRVQSVNVGGTENVIRACKKQ
                     KVERMIYTSSIHAFTELEPGSVIDENVPFNPARTSGVYGKSKAEAVLNVLTAAREGLN
                     AVVLCPTGIIGPFDYKLSEMGNMIRLFASGRLRVGVDGSFDFVDVRDVVHSEIEASVK
                     AKPGEVYIIGGESVSIQELTDILHQITGKKRIGTFLKSTSAYVLSAFSTLYYMISKTK
                     ACFTPYTVHTLTRNYKYSHEKASKVLGHNPRRVSESLRDAVEWLSGYGTIKLQSKLDS
                     HRPM"
     gene            complement(3601..4086)
                     /locus_tag="PKL93_10395"
     CDS             complement(3601..4086)
                     /locus_tag="PKL93_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNQ71707.1"
                     /translation="MTVRTKELGPDKILEAVFDLVRNFTKFFSLNVEAEEMKTIELYI
                     LLFVALKGPQSMSSLAKEYSMTKSNVTFLVDDMESKGYLARIRSEEDRRVIMITLTER
                     GKAFFESFLKNFSRLISTFIRKVGAEDLAIITDGFERITRIVIDEELVSSDEGGKPDK
                     S"
     gene            4208..>4802
                     /locus_tag="PKL93_10400"
     CDS             4208..>4802
                     /locus_tag="PKL93_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HNQ71708.1"
                     /translation="MINRRISIFIIALFAVVTVVMGLFILRVEINVSSEFLLPEKSPS
                     RENMRMMEDLFGREKQIIIVVKTDGLFEEENSLRIYDFLQKLSTIEGVVSYNSYQDAA
                     KVSLIGGTNVEPYFENGVAKENAREILQNPLYLDNLLDENGEVALIPINVTENADVKA
                     ILEIAAESFEGMEFYASGEPIIDEELYGSILSLLVVYP"
     assembly_gap    4803..4902
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4903..6453
                     /locus_tag="PKL93_10405"
     CDS             <4903..6453
                     /locus_tag="PKL93_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNQ71709.1"
                     /translation="IIDEELYGSILSLLVVYPPILFGLICFIYFLRLGSVRAAIIPSI
                     LSIVAAVWTYGFGVMIGLNINILTSTAGLFIIIISSSYGLHFIDRYITNRRFLERSAA
                     LKRTVREELIPVFLSALTTAVGFLTFLVSSLEAFRQLGIMVSLGIMVSSALVLIALPA
                     VLVFVDLPAVKRKLRFRAPTREVAKNIDTVVLVVILVFAVVSPFLISKIDRNFDQYDY
                     FKKSSSIVKSAETIKEEFGWNMPFYVILNKNGLFTGADASVLSKLIVEINELEEVRGV
                     SSIMDVSSAFGIPLPILQIAARNGDLPVEQYMVGNSIRLLVKTPNTDAVNSQRLENEL
                     KEILEDYPQYSPYVASPLLIISGVNNEILSSQVTTIIWALVVITLLLIIVFRSFKLAL
                     VSVLPIALSVFFNFSYMSILGIKLEISTAIVAGVLLGMTIDYAIHLINRFMDTKDIYR
                     AREEVRPAILSNTIALAAGFASLLFAPLRLFSGLGLLLAIGMLTGAIVTLTIIPKIVS
                     KWKMSTHS"
BASE COUNT         1735 a         1598 c         1636 g         1867 t
ORIGIN      
        1 aaatcattcc ttagaattca agtcatagct tatatatcta caacagacca caggatccgg
       61 tcagtcccta ttttcgctcg cagcgttcag cgcttctttg atccggatgg tgaaacaagt
      121 tcatgactga atgtggcgtt tccgaaatga ccacaccttg tcatcccgaa cttgtttcgg
      181 gatccggttt tgatcttgtc agcgcatagc ggttcatggg tacgagatcc cgtacaggat
      241 cactacggga tgacattcct aaacgtcatc ctgacaatgc tcctggtcag gatctctgcc
      301 cctcgcgaaa aagggacgta aggagaatcg tcagtccctg tttccgcact cgtctctata
      361 cagcataccg atttatcgga tctacctttt atcgaagttg caagcgcttc acgtgctccg
      421 attgttaccg ggaacgcaaa acattttccc aaggatttgg tgagagagat tagtccggca
      481 gtattagtaa agactattca taaaaagatt agaagcagaa agagtattat gagtccgtca
      541 taataactcc cattcggagt actatagtaa cgtcaagttc tgaaagcgtg gttttcgatc
      601 cctctgcttc tgggaaacgc ttagagattc ttgatgagca agctggttta tgatttcagt
      661 tcgttgccct ttgaatcact ttgattctta tccaagttgt atcattactc tttctagcga
      721 ggtgtcgatt atgaaacgcg tggcttttat ggtccttgta gcgattgcag ttgtcatttt
      781 gctcagcgcg tgtccatccg ttgcgaaaac cagaagtctt gtaggcacat ggaatctttc
      841 agtgatcaaa agtgaatcag aaaccttcac ggccttcatg cagattacca cccacgagaa
      901 gggcaatctc tccggttatc tggggaacca cgagtcctat ggtaattatg gcacaatctc
      961 cggtacgact tcgaaggaca agatcatctt tctctattca agcgacgagg gaggagagac
     1021 gattcaattc acaggaaaat tcgactcaaa cgggatgaca ggcaccgcaa ctgtttatat
     1081 taacggcagt tacaagtcgg gacctcataa ctggacggcc cataggtagg aatagcgaaa
     1141 agagccgttc ttcgagccta cgtatctaga ttcctggtta gttaaggagc gtaagagtcg
     1201 tccttggtcc ttcgggatca ttaacccgtc cttcagaaga gcaagaagtc gagagggcta
     1261 gacggacaac gcgcgaaaag cgggacagac cataggaaca tttgggaacc agtctgctaa
     1321 cgcaggccag tcaggctacg cctggccagt ctcgcctttg gcgaggccag ttccggctac
     1381 gctgggcaaa aagagacgct cgtataagag acgctgaagg ctgtacgctg gaaaacaaag
     1441 tatcgggtta gaagagcgcg ttaggtaata cgggtaacta gaggcgggtc atcagaacag
     1501 ggttttgaag agaaggatgc tgaaacaagt tcagcatgac gcgaagggca gggtatcgat
     1561 tacaggatat ggggtctcgt gaagagaacc ggcaatcaat tcagggtttc aatgcaactg
     1621 aggaagttat cccgacatga ctttttgaaa ctccctctcc ttgtcatccc gaacttgttt
     1681 cgggatcttg gtgttgatct tatccaagga cgggtccatg atcttggacg ggcgacgaag
     1741 gacggctttt tccagcgtcc agcggttctt agaacgggat gtgaaacaag ttcagatact
     1801 ctaagttgtc aagtatagaa ctaggaaaat tatgagagtg aaaggtcaaa tggaacgtta
     1861 tttatcaaaa gttggtagac catttcagct gcatgactga atgcggcgtt tccgaagtga
     1921 cctcacattg tcatcccgaa cttgtttcgg gatcccgttt tgatcctctc gaaggacagt
     1981 ggaccgttga cggacaacgt tgtcatcaca gcgttcagcg gtttttggtt cttaagcgta
     2041 cagcagttct ttgggcgaga tcgcgcacag gagcactacg ggatgacaaa gtgagttgat
     2101 ttggggcagg ctttcccttc acgtcatcct gacaatgctc ctggtcagga tctctgcccc
     2161 tcgcgaaaaa gggacgtaag gagaatcgtc agtccctatt ttggctctcg tcttctcgtg
     2221 agcgaagcga actctcgaca atgctctttc aagaacctgg atgctgaaac aagttcagca
     2281 tgactgaatg cggcgtttcc gaagtgacct cacattgtca tcccgaactt gtttcgggat
     2341 cttggtgttg atcttatcca tggacgggtc catgatcttg gacgggcgaa ggaccaagga
     2401 cggctcttca cagcggtcag cgtcttctag caagcgttca gcggttctta gaacgggata
     2461 ctgaaacaag ttcagacgtg atgtgatgtt gttaactggc gcgaataata gtaagaagct
     2521 tctcttttca gaataagttc tcgtgcattc tgtcgtaatc aaattactgt tgccagatgg
     2581 aaagggttta catcggacgg tgagagtcta gctttgactg cagtttgatc gtgccgtatc
     2641 cggatagcca ttcgacggcg tccctcaggg attccgagac tctccttgga ttgtggccca
     2701 atactttaga ggctttttcg tgagaatact tgtagttcct ggtgagtgta tggaccgtat
     2761 acggagtgaa acaggccttc gtttttgaga tcatgtaata gagtgtacta aaggccgaaa
     2821 gcacataggc gctagtgctt ttcaagaagg tgccgattct ctttttgcct gttatctggt
     2881 ggagtatgtc cgtaagctcc tgtatcgaga cgctctctcc gccgataatg tagacttcgc
     2941 ctggctttgc cttcacggag gcctctattt cagaatggac gacatcacgg acatcgacga
     3001 aatcgaagga tccgtcgacg ccaactctaa gcctaccgga ggcgaagagc cttatcatgt
     3061 tgcccatctc tgaaagcttg taatcgaatg ggcctatgat tcctgtcggg cagagtacaa
     3121 cggcgttcag gccttctctg gcggccgtca atacattcag cacggcttct gccttggatt
     3181 taccgtagac gccgctagta cgcgctgggt tgaatgggac gttttcatct atcacgctac
     3241 ccggttcaag ctcagtaaag gcatgtatcg agcttgtgta gatcatcctt tcgactttct
     3301 gcttcttgca tgcgcggatt acgttttctg tgccgccgac atttacactt tgaacccgct
     3361 ttttatctgt aggcataatg gatatgagcg ccgccaaatg atagaccgtg tcgattcctt
     3421 cgcaggcttc tatcacaaag tctctgtcgc ggacatcgcc tcggaggaca gttacaccct
     3481 ccagctctat catagaggta tcttctccag ggagagcaac tgccctcacg ctttcacctc
     3541 tgctgacaag ctctctgacg agaacgttac caacgtgtcc ggtggaaccg gttaccagta
     3601 tcatgactta tcaggtttgc ctccttcatc ggaactgact agttcttcgt ctataacaat
     3661 ccgggttatt ctttcaaatc cgtcagtgat tatcgccagg tcttccgcac cgacttttct
     3721 tataaacgtt gagataagtc tggagaagtt ctttaaaaag ctttcaaaga aagccttacc
     3781 gcgttcagta agtgtaatca tgatgaccct tctgtcttct tctgaacgga tacgggcgag
     3841 atagcctttg ctctccatat cgtcaacgag gaaggtgaca ttgctctttg tcattgaata
     3901 ttcctttgca agtgaggaca tgctctgggg ccccttgagt gcaacgaaca aaagaatgta
     3961 gagctctatt gtcttcatct cttcggcctc aacgtttaag gaaaagaact tcgtgaagtt
     4021 ccttacaagg tcgaacaccg cttccagtat cttgtccggg cctaactctt tggttcttac
     4081 agtcattcca cacctccaac gagataatta taacagttta actgctgaac tatcaacatc
     4141 ttttactgta catatacatt actttccaag aaatagtagt aaaattttgt aaagggggca
     4201 atccactatg attaatcgaa ggatctctat ctttataata gccttgtttg ccgtcgttac
     4261 ggtagtgatg gggctcttca ttcttagagt tgaaataaac gtttccagcg aattccttct
     4321 cccagagaaa tctccctcta gagaaaacat gagaatgatg gaggatctat tcgggaggga
     4381 aaagcagatt attattgtgg tcaagacgga cggtcttttc gaggaagaga actcactcag
     4441 gatttacgat ttcctccaga agctctctac gatagaggga gtagtcagtt acaactctta
     4501 tcaggatgcg gcgaaggtta gtttgatcgg cgggacgaat gtcgagccat attttgagaa
     4561 cggggttgcg aaggagaacg ctcgcgagat actgcaaaac ccgctctatc tcgacaacct
     4621 tcttgacgag aacggtgaag tcgcactgat tcctataaac gtaactgaga atgcagatgt
     4681 gaaagcgatc ctggaaattg ccgctgaatc atttgaggga atggagttct atgccagcgg
     4741 tgaaccgatt atcgatgaag aactttatgg ttccatatta tcgcttctgg tagtatatcc
     4801 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattatcga tgaagaactt
     4921 tatggttcca tattatcgct tctggtagta tatcccccca tcctcttcgg attaatctgc
     4981 ttcatctact tcctgaggct cggaagtgtg cgggcggcaa tcattccctc gattctttcc
     5041 attgtcgcgg ctgtttggac ctacggcttc ggcgtgatga taggtttgaa tataaacatt
     5101 ctgacttcaa ctgcgggcct tttcataatt ataatctcat cgtcttatgg gctgcacttc
     5161 atagataggt acatcacaaa caggagattt ctcgaaagga gcgctgcgct taagcggacc
     5221 gtacgcgagg aactgatacc agtttttctc tcggcgctta caacggccgt aggttttctt
     5281 acattcttag ttagcagctt ggaagccttc cggcaactgg gcataatggt ctctctcggg
     5341 ataatggtga gttcggcgct cgtactcata gcgcttcccg ccgtgctcgt tttcgtcgat
     5401 ctgcctgcgg taaaaagaaa gttaaggttt agggctccaa cacgagaagt cgcaaagaac
     5461 atcgacacag tggtgctcgt agtaatactc gtctttgcag tcgtctcgcc ttttctgatt
     5521 tcgaaaatcg atcggaactt cgaccagtac gattacttca agaagagctc gtcgatcgtc
     5581 aaatctgccg agacgataaa agaggagttc ggttggaaca tgccctttta cgttatcctg
     5641 aataagaacg gcctctttac gggcgccgat gcttcggtct tgtctaagct cattgtcgaa
     5701 ataaacgagc tcgaagaggt caggggagtt tcatctataa tggatgtttc aagcgctttc
     5761 ggtattccgc ttccgatact tcagatagcg gctcgcaacg gcgatctgcc cgtcgagcaa
     5821 tatatggttg ggaattcaat acggttgctt gttaaaactc ccaataccga tgcggttaac
     5881 tctcagcgtt tagaaaacga gctaaaagaa atactggagg actatccaca gtattctcca
     5941 tacgtagcgt cgccactgtt aatcatatcc ggtgttaata acgagatact tagcagtcag
     6001 gtcaccacaa ttatctgggc cttagtagtc atcactctgc tgcttattat cgttttcagg
     6061 tctttcaagc tggcacttgt gtcggttctt ccgatagccc tgtcggtatt cttcaacttc
     6121 tcttatatgt cgatactggg cataaagcta gaaatctcta cagcaatcgt tgctggagta
     6181 cttctgggaa tgacaataga ttacgcaatc catttgatca acagattcat ggacaccaaa
     6241 gacatctaca gggcgagaga ggaagtcaga ccagcgatac tctcaaacac gatagctctc
     6301 gctgcgggat tcgcctcgct cctgtttgca ccgttgagac tcttctctgg actcggactt
     6361 ctgcttgcga tcggaatgct taccggagcc atagtaactc tcacgataat accaaaaatc
     6421 gtcagcaaat ggaagatgtc cactcattca taactcgctt ataaagagat agaaaagtct
     6481 gtccgagcct ttgacagagg cctgtcatcc cgtaatgctc ctgtacggga tctcccatca
     6541 aagactcgct tttcgctgaa cgctctcaag acaatgttgt ccgtcaacag tccaccgtcc
     6601 tccgcgaaga aaaaacaaag atcccgaaac aagttcggga tgacagtgtg aggccacttc
     6661 ggaaatgtcg cattcagtat ctgaacttgt ttcagcatcg cgttctaaga cccgctggtc
     6721 gctgaaaacc tagaacagcc gagaaagaac gcatttgaga cgtccgcttc gctcacgaga
     6781 agacgagagc gaaaataggg actgacgatt ctcctcacgt ccctttttcg cgaaaggcag
     6841 ggatcctgac caggagcatt gtcaggatga cgtttagcgg tgtcatcccc taattccccc
     6901 atattgtcat cccgtagtgc tcctgtacgg gatctc
//
LOCUS       DAOQYI010000075         6715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_11762_length_6715_cov_68.818468, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000075 DAOQYI010000000
VERSION     DAOQYI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11762_length_6715_cov_68.818468"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKL93_10410"
     CDS             complement(<1..743)
                     /locus_tag="PKL93_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71710.1"
                     /translation="MKRFLLPFLIVISVVLSAKTGLFLSVYPVLSLNNYTVHDRGYQP
                     DFDDLRSYFSYPAFATAGVELSGSDYRALFRLDFRQDITSFLRGRSWSNFPIRHNAYT
                     LYMDTDLPRVGFAEFENDFLRISAGKRKLHFGPATYNFVLSETVPYFEHLWLDLGEEV
                     LSGKYFYSFFVISSDRSIFNSPRTLIGHSIGFEGSLARIGIVETNLISNTFPDLRDMS
                     PFMVFHNNYAKNSNVNAGLFVESKIGSLD"
     gene            complement(898..2187)
                     /locus_tag="PKL93_10415"
     CDS             complement(898..2187)
                     /locus_tag="PKL93_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71711.1"
                     /translation="MDKKFSMGRGVLLLIVFVLLSYIFTSMTVWTTDGKYLFVSRYLR
                     INQHKRVLSGENFAPDQYRFGSYYLVENLFKFIPLEWLDENSEELSMMLLSGDYWTEE
                     KKGMIDSYFPLVEREKLVSDLEKVIEELVDSVTGKNLLLNNALKAFASSLNWQVYLTD
                     PATTALLIGERLPEDIKSAVDLSSYENRILNGHITARFFFNVLTLLLLYNFCRLFSSP
                     SESLLSTVVFQAIMPLTTMYFGWETFHAAALFIGGLLLIAKGGRFYLLCLLMVLGSLF
                     RPDHMIFLSLIYLLFNFNKGLSWSKRALILSKSLIAGVIPAALTFVVSRFLYPNAEYS
                     VDLIQLRYNITYIWSWIYPLIFASIPLLFLREVKRYSFFRKTWFWILPFIVMNFLVAK
                     TAEVRLFTPVIVFFAPLIGIGLQRFFPGRSMENTAIE"
     gene            complement(2341..3654)
                     /locus_tag="PKL93_10420"
     CDS             complement(2341..3654)
                     /locus_tag="PKL93_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71712.1"
                     /translation="MADHHLKFALSAKARSTAVVFLFVFVLSYVFTSVSVWTTDSRFI
                     TVSRFIRVYGHENLIRGTGYAPEQYRFGGFYLVENFFKYIPLKWYDVYNSNLSGLLTS
                     EEAWTDEMQKNVDKFFPQDDREEMIGEVQRVIDETLESFFPGNALVQNLLRGMIDGLQ
                     WQSYLTNIEETLLTLGEMIPENIRNHLLEDSEETRLVNGYFTSRFFLFMILLTIIYFL
                     CREFLNPVQSLFGVVLFAALVPIALQDFLQAETVLSLLLFSSMLLLTKRDGSRLILFL
                     VTILCCTARTDHALFGALIYGLIHGTESLRRRQWSRALFSALLLIIPVVATALISGFL
                     FPEAEYYVDLIQFEFNITHIWSWIFPSILLLLPIVFFSQIKHFEFYRKTWPWIPLFVG
                     TNFVLGKTAEVRLFLPLVIYSIPLVIGGVIRSLEEEKNSANSREV"
     gene            complement(3697..4779)
                     /locus_tag="PKL93_10425"
     CDS             complement(3697..4779)
                     /locus_tag="PKL93_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ71713.1"
                     /translation="MKKTRVAIIKTKPETVLDDYRRLAKLAGMGEALDKTATTILKDN
                     ISWHLPMPGANSTPWQLEGVVLALKDEGMNDIVSVENKTVVTRPKYGEKQNKYDIVYD
                     KFDIPVLYNFESKDMTWIEYTPKSKLMVLPKIFPEGIKLPDYFFGKNIIQLPTMKCHI
                     YTTTTGAMKNAFGGLLNVKRHYTHSWIHETLVDLLKIQKEIHTGLFAFMDGTTAGNGP
                     GPRTVIPVKADYILASEDQVAIDAVAAKMMGFNPMDLKYIRLADEEELGNGRPENIEL
                     VGDDISGVNFKFHVGDNFASRVGDLLWFSPLKVFQKLFFHTPLVKIFVAASDTYHDKY
                     WWKKHGVKIFEEWKKESPWGRLWESY"
     gene            complement(4793..5164)
                     /locus_tag="PKL93_10430"
     CDS             complement(4793..5164)
                     /locus_tag="PKL93_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HNQ71714.1"
                     /translation="MNLTEALILVIAIVFNAVANILLKHGMQNAPDIGSVGLKGMLIN
                     SVTNISVWLGLISFGVAFIFYSVVLTKMRLGVAYPIMTSAGFAIVTVAAVFLFDERLS
                     AMKIVGIAVIALGIWLVSVAK"
     gene            complement(5178..6062)
                     /locus_tag="PKL93_10435"
     CDS             complement(5178..6062)
                     /locus_tag="PKL93_10435"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNQ71715.1"
                     /translation="MMAFSIFLRLIRVKQWLKNLFVFAPLIFSSSLTDTVSVLRAILI
                     FFAFCLASSGVYILNDIRDRESDSHHSRKKERPIASGQVRVSSAVIVSLLLFGSAAVI
                     SSLLPFLSSLFLLLYIVVNLFYTLKGKELVLIDAFCISAGFVIRVMAGAYAIETTPTG
                     WIVVTTFFLSLFLGFGKRRNELLSLKEGSNDHRKVLSLYNDRYLDYLMIATASITIIA
                     YTLYCLDPSTILKFNTDKFVYTVPFVTYGVFRYLLLLFRNGEGDPTEVVTRDTGIVLT
                     VLFWIVSVMLVIYLPSFL"
     gene            complement(6179..>6715)
                     /locus_tag="PKL93_10440"
     CDS             complement(6179..>6715)
                     /locus_tag="PKL93_10440"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNQ71716.1"
                     /translation="AVDLRDPYTAGHQRRVAELSVAIGKKLGLEEDRIRGLRLSAMIH
                     DVGKIQVPAEILSTPRRLTDLEFDLIKLYPAAGRELFKDIEFPWPVADAIYQHHERLD
                     GSGYPEGITGEKIILEARIIAVADVVEAISSHRPYRPSLGIDAALDEIRKGKGRLFDP
                     EVVDACLELFDEGFSFSD"
BASE COUNT         1952 a         1604 c         1408 g         1751 t
ORIGIN      
        1 aaatccagac ttcctatctt ggattcgacg aacaagcccg cgttaacgtt ggagttcttt
       61 gcgtagttgt tgtgaaagac catgaagggg ctcatgtctc tcaggtcggg aaacgtattg
      121 ctgatgagat tcgtctcgac gataccgatt cttgctaaac tgccctcaaa accgatgctg
      181 tggccgatga gcgttctagg gctattaaaa attgaacggt cagaagatat gacgaagaag
      241 ctgtagaagt acttaccgga taaaacctcc tcgccaagat cgagccagag atgttcgaaa
      301 tagggcactg tctccgacag cacaaaattg taagtcgcgg gaccgaagtg aagttttcgc
      361 ttaccggcag aaattctcaa gaagtcattt tcaaactcag caaagccgac cctgggaaga
      421 tctgtatcca tatagagagt ataagcgttg tgcctgatcg ggaagttcga ccagcttctt
      481 cctcgaagaa aagaggtaat gtcctgcctg aagtcgagcc tgaacaatgc ccgataatcg
      541 cttcccgaca actccacgcc ggccgtcgca aatgccggat aagagaagta actgcgcagg
      601 tcatcgaagt caggctgata ccctctatcg tgtactgtgt agttgttcag ggagagaaca
      661 ggataaacgc tcaaaaaaag ccccgtcttt gcgctcaaaa caacggaaat aacgatcagg
      721 aacggcagta gaaatcgttt catataaagc agcctcctct tttgattata cgagattatc
      781 ccataagttg gctagaattc ttctttcaaa aggcaatcga aaaaaacttg cagttcagcc
      841 caaaatgagt tgtatagaat acgatgcacc aatcgggctc gacagagagc ctctggatta
      901 ttcaatcgcc gtgttctcca tggatctgcc ggggaaaaac ctttgcagcc ctatccctat
      961 gagcggcgcg aaaaaaacga tgactggagt gaacaatcta acttcagcgg tttttgctac
     1021 aagaaaattc ataacaatga acggaagaat ccagaaccat gtcttcctga aaaagctgta
     1081 gcgcttcact tctctaagaa acagcagtgg aatcgacgca aagatcagcg ggtagatcca
     1141 tgaccaaata taggtgatat tgtatctcaa ctgaattaag tccacagagt actccgcatt
     1201 cggatataaa aaacgactga cgacaaaagt tagagcagct ggaatcacgc cggcaattaa
     1261 actcttggac agaattaacg ctcttttcga ccaggagagg cctttattga aattgaagag
     1321 gagatagatg agggagagaa atatcatatg atccggcctg aaaagcgatc ccagtaccat
     1381 caacaggcaa agaagataga atctgccccc tttggctatc aatagcagcc caccgatgaa
     1441 aagagctgca gcatgaaaag tctcccagcc gaaatacatg gtcgtaaggg gcattatggc
     1501 ctgaaagacc acggtcgaca gcaatgattc ggaaggcgaa gagaagagtc tgcagaagtt
     1561 gtacaggagt aggagggtca aaacgttgaa gaagaatctt gccgtgatat ggccgttcag
     1621 aatccggttc tcataactcg atagatccac agcacttttg atgtcctcgg gcaacctttc
     1681 accaataagg agagcggtag ttgcagggtc cgtcaagtag acctgccagt taagactgct
     1741 tgcaaatgcc ttgagcgcat tgttcaagag aagattcttg ccggttacag aatcgacaag
     1801 ctcttcgatg accttctcta aatccgagac gagtttctct ctttcgacca gcggaaagta
     1861 ggagtctatc ataccctttt tctcttccgt ccagtaatct cctgataaca acatcattga
     1921 aagctcttca ctgttttcgt cgagccactc taagggaatg aatttgaata ggttttccac
     1981 aaggtagtaa cttccaaatc tgtactgatc aggcgcgaaa ttctctccgg aaagtactct
     2041 tttatgctga tttattctca ggtatcttga tacgaagaga tactttccat ccgtagtcca
     2101 cacagtcatc gatgtaaata tgtaggaaag caagacaaaa acgataagga gaagaacccc
     2161 ccgtcccatg ctaaattttt tatccacggt tcacctccag atttcttcgg attactgcag
     2221 ttcgcgcttg aacgtagaga taataccatt gcatttttca aatcatgaaa ttctgatctt
     2281 tctgtgcgat gcctgctcca aagcttgtgc aacatattgg atcgaaagca gacttatcgc
     2341 tcacacctcc cggctgttcg ccgaattctt ctcttcttcg agagatctga tcacacctcc
     2401 tattacaaga ggaatggagt aaataactag aggaaggaac aacctgactt cggcggtctt
     2461 tccaaggacg aaatttgtcc ctacgaacag aggaatccac ggccatgtct ttctgtagaa
     2521 ttcgaaatgc ttgatctgtg agaaaaagac aatcggtaac agaagaagaa tcgaagggaa
     2581 gatccagctc catatatgag taatgttgaa ctcaaactga attagatcga catagtactc
     2641 ggcttcagga aagagaaaac cgctgataag agccgtcgca accacaggta ttatcagaag
     2701 cagcgcagag aatagcgctc ttgaccattg tcttcttcta agagactcag ttccgtgtat
     2761 cagaccataa atcaacgcgc caaacagcgc atgatccgtt cttgccgtgc aacagagaat
     2821 cgttacgaga aataaaatta gcctacttcc gtccctcttt gttagcaaga gcattgagga
     2881 aaacagcagc aatgaaagga ctgtctcggc ctgcaagaaa tcttgaaggg cgattggaac
     2941 cagagcggcg aagagaacca ctccaaacag cgactgtaca gggttcagga actctctgca
     3001 cagaaagtag attatcgtca gaagaatcat gaagaggaag aatctcgatg tgaaatatcc
     3061 gttcacgagt cttgtctctt cgctgtcctc tagcagatga ttcctgatat tttccggtat
     3121 catctctcca agagtgagca gggtctcttc gatattcgtc aaatagctct gccattgcag
     3181 accgtctatc atgccccgca gaagattttg aaccagagcg ttgcccggga agaatgattc
     3241 taaagtctca tcaattaccc tctgaacttc gccaatcatc tcttccctgt cgtcctgggg
     3301 gaagaacttg tcgacgtttt tctgcatctc gtctgtccag gcttcctcac tagtaagcaa
     3361 accggaaagg ttgctgttat atacgtcata ccattttaaa gggatgtact tgaagaagtt
     3421 ctcaacaagg tagaatccac cgaagcgata ttgctccgga gcataccccg tgcctctgat
     3481 taagttctcg tgaccatata ctctaataaa tcttgacacc gttatgaatc tgctgtctgt
     3541 cgtccagaca ctgacagagg tgaagacgta tgacagaaca aagacaaaca aaaagacaac
     3601 tgcggtgctt ctcgctttcg cactcagggc aaatttcaag tgatgatcgg ccatgatttc
     3661 cccacccaag ctttcattcg cacaatgaat tgtctatcaa tagctctccc ataaacggcc
     3721 ccagggactc tctttcttcc actcttcgaa tatcttgaca ccgtgcttct tccaccagta
     3781 cttgtcatga taagtgtccg aagcggcaac aaagattttc acaagcggtg tgtggaagaa
     3841 gagtttctga aagaccttca acggactgaa ccagagcaga tctccgactc tgctcgcaaa
     3901 gttgtctcca acatggaatt taaagttgac accggaaatg tcgtctccga caagttctat
     3961 gttctctggt cttccatttc cgagttcctc ttcatcggcc agcctaatgt atttcaggtc
     4021 catcgggttg aaacccatca ttttcgctgc gacggcatct attgcgacct gatcttcgct
     4081 ggcaagtatg tagtctgcct tcacgggaat cactgtccgc ggtcccggcc cattccctgc
     4141 ggtcgtaccg tccatgaaag cgaacagacc cgtgtgtatc tccttctgta tcttcagcaa
     4201 gtcaaccagt gtctcgtgta tccatgagtg ggtgtaatgc ctcttaacgt tgagcaggcc
     4261 accaaacgca tttttcatcg caccagtagt tgtcgtatat atgtggcact tcatcgttgg
     4321 gagctggatt atatttttcc cgaaaaagta gtctggcagt tttattccct ctggaaagat
     4381 ctttggaagt accatcagtt tgcttttcgg tgtgtactca atccaggtca tatctttcga
     4441 ttcgaagttg tacagaacag ggatatcgaa cttatcgtag acgatgtcat acttgttctg
     4501 tttctctcca tacttcggtc tcgtaacgac ggttttattc tcaaccgata cgatgtcgtt
     4561 cattccctca tcctttaatg ccagcacgac tccttctagc tgccatggtg tactgttggc
     4621 accaggcata ggaagatgcc aggatatgtt gtccttcaag attgtagttg cggtcttatc
     4681 taacgcttca cccattccag ctagcttggc cagtcttctg taatcatcta atacggtttc
     4741 aggtttggtc ttaattatag ctactctcgt ctttttcatc gaaatacctc catcattttg
     4801 caactgaaac aagccagatt cctaaagcaa taacagcgat tccgacaatt ttcattgcac
     4861 tgagtctttc atcgaacagg aaaactgctg caacggtaac gatcgcaaat cctgcacttg
     4921 tcattatcgg gtatgcaact cccaaacgca tctttgtcag aaccacacta tagaagataa
     4981 atgcaacacc aaatgagatc agtccaagcc atacgctgat gttagtaaca gagtttatca
     5041 gcatcccttt aagtccaacg cttcctatat caggcgcgtt ctgcatccca tgtttcagca
     5101 agatgtttgc tactgcatta aagactattg ctatgacaag aatcaaggct tcagtcagat
     5161 tcattcaaca tcccccttca cagaaaactt ggtaaataga ttaccagcat aacagatact
     5221 atccaaaaca gaacggtaag cacgatccca gtatctcttg tcacaacctc cgttggatct
     5281 ccttcgccgt ttctgaaaag gagaagcaga tatctaaaaa ccccgtatgt cacaaatgga
     5341 acggtgtata caaatttatc cgtgttgaac ttcagaattg tcgatgggtc caagcagtaa
     5401 agcgtatatg cgattatcgt tatagatgcc gttgcaatca tcaaataatc aagatatctg
     5461 tcattataaa ggctaagcac ctttcgatga tcattgctac cttctttcag agaaagcagt
     5521 tcgttccttc ttttgccgaa cccaaggaaa agtgagagga agaatgtggt caccacaatc
     5581 catccggttg gagtcgtttc aatagcgtat gcaccagcca ttactcttat cacaaaaccg
     5641 gcagaaatac aaaaggcgtc tatcagtacc agctctttac ccttcaaggt atagaaaaga
     5701 ttcacaacga tatatagcag caagaagagc gatgacaaaa aagggagtaa agaactaatc
     5761 actgccgctg atccaaaaag caataaagaa acaatcacag cgcttgaaac cctgacttga
     5821 ccagaggcta ttggtcgttc ttttttcctt gagtgatgac tgtcactctc tcgatctctt
     5881 atgtcattca aaatataaac gccgctagaa gcaagacaaa aagcaaagaa gatcagtatg
     5941 gctcttaaaa ccgataccgt gtcggtaagg cttgaagaga aaatcaaggg agcgaacaca
     6001 aagagattct tgagccactg tttcaccctt attaacctta gaaatatcga aaaggccatc
     6061 attttcaact cctacggcga caagacttca ccattcgcgt tatattcatc ttttctcaca
     6121 gtgtagcagt tctcactaga atacatagta ttcaaataat ctgaccggaa gcagtaaatc
     6181 agtctgaaaa ggaaaatcct tcatcgaaca attcaaggca ggcatctact acttctggat
     6241 cgaagagcct gcctttgccc tttctgatct cgtctaaggc cgcatcgata cctaacgaag
     6301 gcctgtaagg tctgtgcgat gaaattgctt ctacaacatc ggccacggca attattctcg
     6361 cttccaaaat gattttttct cccgtgatgc cttcagggta tccgctgcca tcgagtcgct
     6421 catgatgctg gtaaatcgca tctgcaacag gccaaggaaa ttcgatatct ttgaaaagct
     6481 cacgacccgc tgcgggataa agttttatta gatcgaactc caaatccgtg agccttcttg
     6541 gtgtactcag aatctccgcg ggcacttgaa tcttccccac atcgtgaatc atcgcagaaa
     6601 gccgaagtcc ccttattcta tcctcttcaa gtccaagttt ctttccaatg gcgacggaca
     6661 actccgcaac ccttctctga tgacccgctg tataaggatc tctgagatct acggc
//
LOCUS       DAOQYI010000076         6445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_12391_length_6445_cov_78.792645, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000076 DAOQYI010000000
VERSION     DAOQYI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12391_length_6445_cov_78.792645"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..280)
                     /locus_tag="PKL93_10445"
                     /pseudo
     CDS             complement(1..280)
                     /locus_tag="PKL93_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(453..653)
                     /locus_tag="PKL93_10450"
     CDS             complement(453..653)
                     /locus_tag="PKL93_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71717.1"
                     /translation="MPPVKYHVRFSRINPIESVRHSIALDKILIPEGFKQLLTTDARK
                     EFRVPSSSTEEGPDFSKARLQR"
     gene            complement(1039..1377)
                     /locus_tag="PKL93_10455"
     CDS             complement(1039..1377)
                     /locus_tag="PKL93_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71718.1"
                     /translation="MRCFETNLESFHRIARKGRDLDLKIAAEVMKKKPMEIIAEHEHL
                     DSLLEYIDLDCIETTFDIAHATSFFKNLVDGEALNGMIINCMRRLRKMHNVHLSSATS
                     AKVDTPLLRG"
     gene            2073..3191
                     /locus_tag="PKL93_10460"
     CDS             2073..3191
                     /locus_tag="PKL93_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNQ71719.1"
                     /translation="MKNVFLYVIGAVVFGYVLFTFIDASANDQSTIKGRVIEMISFDE
                     TGGYSAKGLQTLRVKVLTGTYAGEEVVIENHFTDNPAMDIRVEKGDTVVLYMDERDEV
                     TFHIIDFVRQRYVFAIVLVFICLLLLIGRISGLKSFITLTLTGLIVIKVMLPLILKGF
                     NPIGASSLSAAVITILTFAIVAGVNTKSLSAIFGTLCGVLSASLIAYVTGSLANLTGL
                     SSDEATMLVYIPQNIEFDFMGLLFAGIIIGTLGAVMDVAMSISSAMYEMKSINPEMTT
                     GKLVKSGFNIGRDIMGTMTNTLILAYTGSSIFLMLVFLAYETTVIRIINMDVIATEII
                     RAVSGSIGIILSIPATVLLSAFLLNKYGVRSGSTAGKQ"
     gene            complement(3475..5139)
                     /locus_tag="PKL93_10465"
     CDS             complement(3475..5139)
                     /locus_tag="PKL93_10465"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNQ71720.1"
                     /translation="MRKTLLVLIVVAAIVSLGWACTTVVVTKGASVDGSVMTSHSCDC
                     GECDFRYVYIPAADFEAGSKRPVYPFHEPYPRYIGKDMGPTYDDPNFSPYEPLGYIDQ
                     VEHTFAYYEAAYGVINEHQVAIGECTCSAKVYAQPVANECIFDISALSKVALERCTTA
                     RDAVKLMGDLAVEYGYYGWGETLTVTDPNEAWVFEVCASPDKKSALWAAKKVPDGEVF
                     VEANIFRIRELDPENPDMMFSPNLIEVATEAGWYDPSTGPIDWMATVSTGEYSQPYYS
                     LRRIWRVLDRVAPSKEFSPWVEDGFTTDYPFSVVPDEKLSVADVISLFRDFYEGTEFD
                     LTEGLAAGPFGNPNRYAGSSKLIKGSWERAISIFRCDYVFVSQVRSWLPDPIGGVIWF
                     GAAAPHESILVPLYCGINDVPYAFDHGNLHEFDRNVAGWAMNFMGNWAELKFSYIYPE
                     IQALQQKIEDKLFAVQPAIEAAALKLYEVDPELCKEFLTDYVAGITNDVMDQVWDFNE
                     YLITKYRDGYINIPNVGSSAGYPDWWLDAVGYDEGHIFGDDGYKAK"
BASE COUNT         1766 a         1452 c         1399 g         1828 t
ORIGIN      
        1 gattagttgt ataaaccaat cttctcagtt cctctggata ttgaaagaag gttgatagag
       61 tttcccagtt atccttccag ctttttattg acaagaggta tttgttcccc catttctctt
      121 ctagtgcaag taatgcattc catcctgcct ctcttgtagg tgcctgataa accgtcttta
      181 gatcgtgtgc aagaggttta cgatccctcc aggtaacata tctcatggtg ttccttatct
      241 ggtgaactat gcacttctga acctcagaga agtagctttg tcgattcctg tgcagccata
      301 accttcagct gcatatcggg aagagacgaa tgacatctcc cgcgggaaaa ttcacaagaa
      361 ctaaattgcg cctgtccatt gctcttgcaa cagaactgtc tacttgagtg tttttcatct
      421 cgggacgacc ggcaaaatct tgccagcagg tattatctct gcaacctggc cttagaaaag
      481 tctggacctt cttccgttga agaagaagga acacgaaact cctttcttgc atcagttgtc
      541 aggagctgct tgaagccttc agggataaga atcttgtcca gtgcaatgga gtgtctcact
      601 gactcaatgg gattgattct agaaaatctc acgtgatact tgactggagg cacgaatcta
      661 agacttccga aatgcgtaac tgaaaaacac tgaattcttc gtctacaggg ctttccatta
      721 gctggatttt cccctcgcct ttgcagcaga agagaatgat gctgtgttcg gagattctga
      781 ctattttgct tccacttcag caggcgatca gggggctgaa tccgtaatcg ctaatctcat
      841 aagcttcgta ctgccgatga ttcgcttctt caggagtcgg tagttttttg ttcgacatca
      901 cttagcatct ccttccactt agaagtgact acctcatctt tacgaagaat ctgcattcct
      961 tgtccaggag caaactcttc gattctgata atgccttcgt gattttcgca agattttaag
     1021 aaccgagaag aaatcgtatt aaccccttag caatggagta tctacctttg ccgatgtagc
     1081 actgcttagg tggacattgt gcatctttct aagtcttcgc atgcagttga tgatcatccc
     1141 atttaaggct tctccatcca cgagattctt gaaaaagctt gtcgcatgtg ctatgtcgaa
     1201 agtagtttcg atacagtcga ggtcaatgta ctcgagaagc gagtcgaggt gttcatgctc
     1261 agcaatgatc tccattggtt tctttttcat tacttcagct gcaattttca gatctagatc
     1321 tctacccttt ctggcgatcc tgtgaaaact ctccagattc gtttcgaaac aacgcacgaa
     1381 cctatccttc taagtgaaca tccatccggg atgaacagtt acaacaatag cttcaagttg
     1441 cgctgcaatc tcaagagctt ctatgctctg ttgcatggat tcccttcgta ttccaggatt
     1501 tgaagaggta atatttacat ccctgcttgg agaattaacg gtccgcttaa gaacaaggtc
     1561 gtccagcgtt tctcttgtag cttgcaaatc tctctttcct ttccagagat gtcagcgcag
     1621 atctcgacac ctgttacctt cacatttgcg aaaatcttcg gaaccttctt aaccgggaaa
     1681 tcaaagagaa tgttagaaat gaagagaaac tcaactcgcc agacactgca aatgctgcat
     1741 tgagaactga ctgtcagaaa ctaatgaagt tattgcttcg tcctagaaat accgtttgag
     1801 ttagcgagaa gagaagctcc caagagagcg atttcgttat gcatcaagat ctctgattaa
     1861 gatcactctt ttggaagatc cagtaagcgt cttttttaga acggtgctga tggatttcgt
     1921 ttctcacgtt agatgatcta tggcctcaaa caattcgatg gctatgagac gcatctgaag
     1981 ctaattaaaa gagcaatact acttccttgg cttcgagatg atgatataat ttctaacgtc
     2041 accatgggaa ttcaggagga ttcaagttga gcatgaaaaa cgtttttctt tacgtcattg
     2101 gagcggttgt gtttggctat gtgctattca cgttcatcga tgcttcggca aacgatcaaa
     2161 gtaccataaa aggaagagtg atcgaaatga tcagtttcga tgaaactggt ggctactcgg
     2221 caaagggact acaaactctc agagttaagg tgctgactgg gacctatgca ggagaagaag
     2281 ttgtgatcga gaatcacttc acagataatc cggcgatgga tatccgagtt gaaaagggcg
     2341 atactgtggt gctgtatatg gatgagaggg atgaggtgac ctttcacatt atcgactttg
     2401 ttaggcaaag atacgtcttc gctatcgttc tggttttcat ttgtctgctt cttcttatcg
     2461 ggaggatctc tggactcaag tccttcatta ctcttactct cacgggcttg atagtaatca
     2521 aagtgatgct gcctttgatt cttaaggggt tcaaccccat tggtgcttct tcgctgtcgg
     2581 cggcagtaat cactattctt acctttgcaa tcgttgccgg agtgaacaca aagtccctat
     2641 cagcaatctt tggtacgtta tgtggtgtgc tttctgcaag cttaatagca tatgtaaccg
     2701 gttcgctggc gaaccttacc ggcttaagca gcgatgaagc aacaatgctt gtctacatac
     2761 cccagaatat tgaattcgat tttatgggcc tgctcttcgc agggataatc attggaacgc
     2821 tcggtgctgt tatggatgtt gccatgtcta tctcttcggc aatgtacgag atgaaatcca
     2881 ttaacccaga aatgacaacg ggcaaactcg tgaagtctgg cttcaatatt ggcagggaca
     2941 taatgggaac catgactaac acccttattc tcgcctatac gggaagttca attttcctaa
     3001 tgctcgtctt tcttgcgtat gagacgacag ttataaggat aatcaacatg gatgtaattg
     3061 caacggagat tattagagcc gtttccggga gcataggaat aatactctca atacctgcca
     3121 ctgttcttct ttcggccttt cttcttaata agtatggagt tagaagcggt tcaacggccg
     3181 gaaagcaatg attttcgtac gcatcgattt cttgcacaca aacactactg tcttctcagg
     3241 cttgtaatgg taaaccttca acctgtactg tcaaccattt tcattatctc tagagaggct
     3301 cctgataatc gtttttatcg attgatcata tgtaaataca ggtcacaaca atcttctaat
     3361 tcaacgaaaa aaagtgcgat ttgcaatcag taataatttt cgaaaatcaa aggccgccca
     3421 ctagggacgg cctcttttga acaaccttta atacgaaagg agtgctatga gacgctattt
     3481 tgctttgtaa ccgtcatctc cgaagatgtg accttcgtcg tatccaactg catcaagcca
     3541 ccagtcagga tatcctgccg aagaaccaac attcggaatg tttatgtacc catctctgta
     3601 cttcgtaatt agatattcgt tgaaatccca tacctggtcc ataacatcat tagtgatacc
     3661 ggcaacgtaa tctgtgagga actccttaca gagttcggga tcaacttcgt aaagcttgag
     3721 tgcagcagcc tctattgcag gctgaaccgc gaagagcttg tcttcaatct tctgttgaag
     3781 ggcctgtatc tcaggataaa tgtagctgaa ttttagttct gcccagttgc ccatgaagtt
     3841 cattgcccaa ccggctacat tacgatcgaa ttcatggaga tttccgtgat caaatgcgta
     3901 ggggacgtcg ttgataccac agtatagagg aactagtatc gattcgtgcg gtgcagctgc
     3961 gccgaaccat attactccac cgattggatc gggaagccag cttcttacct gtgaaacaaa
     4021 gacataatca catctgaata ttgaaatcgc cctttcccag ctgcccttta ttagcttact
     4081 ggagcctgcg tagcgatttg gattgccaaa aggtcctgct gcgagtcctt cggtaagatc
     4141 aaattccgta ccctcgtaga aatccctgaa cagagaaatc acgtcggcaa cagagagttt
     4201 ttcatcgggg acaacggaga acgggtaatc agttgtaaag ccgtcttcta cccatggaga
     4261 aaactctttt gaaggagcca ctctgtcaag aactctccag attcttctca aactgtaata
     4321 tggctgagaa tattcacctg tgctaactgt tgccatccag tcgataggac ccgtgctagg
     4381 atcataccag cctgcttccg ttgcaacttc gatcagattt ggagagaaca tcatatctgg
     4441 attttccgga tctagctctc taattctgaa gatgttggcc tcgacgaaga cttcaccatc
     4501 gggaaccttt ttggctgccc atagtgcact cttcttgtcc ggtgatgcac agacttcgaa
     4561 gacccatgct tcgttaggat ctgtcactgt aagagtttct ccccatccgt aataaccgta
     4621 ttcaacggcg agatctccca tgagcttgac tgcatctctg gcggttgtac atctctcaag
     4681 agctaccttt gaaagagccg aaatatcaaa gatacactcg tttgctacgg gctgagcgta
     4741 gacctttgct gagcaggtac attcgccgat ggcaacctga tgttcgttga taactccgta
     4801 agcagcttcg taataggcga aagtgtgctc gacttgatca atgtagccaa gtggttcgta
     4861 aggtgagaag ttaggatcat cgtatgttgg acccatatcc ttaccaatat atcgaggata
     4921 cggttcgtgg aacgggtaaa ccggtctctt tgaacctgcc tcgaaatcgg ccgctggaat
     4981 gtaaacatac ctgaaatcgc actctccaca gtcgcatgaa tgactcgtca ttactgagcc
     5041 atcaacagat gcacccttgg tcacaactac agttgtgcat gcccaaccaa gacttacgat
     5101 tgcggcaacc acaatcagga ctaacaacgt tttgcgcaaa tgaaacccct cctacaaaat
     5161 gatttatggg gaacatcccc agtttagagg cctttgacct cttaaagcac atgaaaaaac
     5221 ctcgccatgt gactacgtca agaaaacaca gcccattagc tgtttcataa gaattgaggt
     5281 gtatcatctc tttcctagag aattgcgtcg tttgaaaggc gcaagtaaga atcattgtga
     5341 gacaagtccg tttcacaaaa ttcgatgctt tgatgagata gtatacaggt actccacagc
     5401 aatgcaagga taaatcgctc tattgtctca gaagcatttt gtctaggatt gcctgcaatg
     5461 agatctctca accacattat aacaggaaat actgtgacaa gctaactttt ttggaatttg
     5521 aaacagaagt tttaaatagg atatatttgc tccgatctaa agtgtggaaa atgaggagaa
     5581 gcagactcga tttgttcgaa tatgagatag cggaataaaa tgcactgaag tgcatccgtg
     5641 aaggcgaagc tggaacgaat tctccaggtg acaattataa cttcgtaggg agtcgattcg
     5701 aacctggtct gccgacgcag aataagaaac gaatttcgct gctcgtttaa cgcttcgaaa
     5761 agacgtcctc cgccgtccaa aatcaatgat ttgctttccg attggatcaa aatcgttagg
     5821 gaagaaagag gcatgagaac agaggcacga agatggagat caaacgacgc aatccgacat
     5881 actgaaagat cagtcgtaag ttctcagttc ctagttggag gttaaagacc cgaaagcaaa
     5941 aacacaacag cccgtcattc tgaacttgtt ttagaatctc gatccttaga gacgctgatc
     6001 gatgtgaaga gccatcccaa aaaagagcag ttcttcgttc cgacgctacg cgtccaggtt
     6061 cttggttctt cgtcagggat tccgtctgaa cttgtttcgg gatctttgga gacaattaca
     6121 acgaatgaat atataagtgg aaggatgaca cagcaaagaa ctgaatgtag tgtttttact
     6181 tttggcacac cgcagtttgc aactgctctc atgaaaaacc aaggacggct cttcacggtg
     6241 cttagcaact ctcagcaagt gcccagcggc tctcaaaacc gtgatgctga aacgagcatc
     6301 acgtgatttc acgttttcag aaagcccgcc gggatactga aacaagttca gacacgatgt
     6361 gacgtattcg cagtcacaac aatgtaatct cgaacttgtt tagggatctg cttttcgatc
     6421 caatcgaagg accggctttc tgagc
//
LOCUS       DAOQYI010000077         6420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_12455_length_6420_cov_76.672427, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000077 DAOQYI010000000
VERSION     DAOQYI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12455_length_6420_cov_76.672427"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKL93_10470"
     CDS             <1..234
                     /locus_tag="PKL93_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71721.1"
                     /translation="DIDIEDPLGMTDGWIIVVNENGELVESLSVGGSADDFILSGVNY
                     GEKYILCGCSTSSDGDLESNKGKKDIWLITLGK"
     gene            299..1963
                     /locus_tag="PKL93_10475"
     CDS             299..1963
                     /locus_tag="PKL93_10475"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNQ71722.1"
                     /translation="MRKILLVLIVAAAIVSIGFACTTIIVTKGASVDGSVMTSHSADC
                     GLCDFRYVYVPPADYEAGAKRAVYPFVEPYPRYVGADMGPTYNDPDLPATEPLGYIDQ
                     VEHTYGYFDAVYGVINEHQLAIGECTCSAKVYAQPKAGECIFDVAALSRVAMERCTTA
                     REAVELMGALAVEYGYYGWGETLTVTDPNEAWVFEICATPDKKSALWAAKKVPDGEVF
                     VESNMFRIRELDPESPDNMFSPNLIDVATEAGWYDPSTGPIDWMATVSTGEYSMPYYS
                     LRRTWRVLDRVSPSLGLSPWVEDSFTKDYPFSIVPDKKLSVADVIDLFRDHYQGTEFD
                     LTEGLAAGPFGNPNRYAGSSKLIKGSWERALSIFRCEYVFVSQVRDWLPDPVGGVVWW
                     GAAAPHETILVPMYCGITDVPYAYDSGSLQEFDYDVASWAFNFMGNWAELKWSYMYPE
                     IQELQKKIEGKLFAVQPAIEAAAAQLYETDPELCKEFLTDYVADVTDRVMAEVWDFNE
                     YLITKYRDGYINIPNVGSSAGYPDWWLDAVGYDEGHIFGEDGYKAK"
     gene            2080..2715
                     /locus_tag="PKL93_10480"
     CDS             2080..2715
                     /locus_tag="PKL93_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNQ71723.1"
                     /translation="MRRFLLVLIVVSLVSVFAFAEPEVKLGLPVVITSAGQSAEVETV
                     NYVADEVGLKYDYCDVLSKDELAAGVGLGGAKSAIGKHVTTLSPDPEGTKFQSLLLVL
                     GASLKGMGASGLSVETEVDRLKDLIEYAKANKIKIIAVAIGGEIQRGLPGSPIEVIID
                     TVVPHADVFIVTKDSNGDGKFTKAAQARNVPIVEIDSAFDLLDTFTQVFGL"
     gene            2781..4085
                     /locus_tag="PKL93_10485"
     CDS             2781..4085
                     /locus_tag="PKL93_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HNQ71724.1"
                     /translation="MSADAIYMQTIIAGIVMVGAYAVGKFLKFSTELCMFVAVIGGAI
                     AGGAGFDTFRHIAEGSVTYLDIGLIFVFATIFMNIIKESGGTNYIVRKIIGAFHNKRI
                     LMLILLMLVILVPGALTGAGSISVLVVGGTVAAALNAMGLSKVRISALIFIVAGLSAA
                     APPVNIWAMMTCAGTAIPYVGFTMPLLIPVLILGLFTVLFFGRGAGNIDKEKALKEIP
                     DPKGLSGWSVGVPFLVLIFLLGAPRIWPFDFPVLGLPLVFAICALVAWLMNFKRVNIL
                     KVSRETVAQLTPLIATTAVVGMLIQVMSFNGVKGLISMWIVTAPLAIVWILLPFIIPI
                     SEGLLTYGGAMVIGIPLIWMLNSNGINPVIVLAGLSLLWPLGDGLPPTALIGRLTVST
                     VGYKGSYGSFLKECVVPWIAITVVAMILVIFANKFNFLMMVG"
     gene            4101..4253
                     /locus_tag="PKL93_10490"
     CDS             4101..4253
                     /locus_tag="PKL93_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71725.1"
                     /translation="MTLIMFLYYVMTAYVVGMLIWNFIKTKDINSEILYALALLPLIL
                     RLLRMR"
     gene            4264..5412
                     /locus_tag="PKL93_10495"
     CDS             4264..5412
                     /locus_tag="PKL93_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HNQ71726.1"
                     /translation="MMRKLLILVVLALIVLAGGIPLYIQRSYKEEVVAGPSVTDVFKL
                     SKYFDGIKGTIADTDVFELKGAEEGGKTLIIAGTHANEPSAALLAYFFIENLEVEKGT
                     VYIIPHFNLSGSLGPQPGGGFPLYYFVDTPWGEKKFRMGDRGFQALDQWPDPDVYVHY
                     PDGQLLSYIDARNTNRSWPGRPDGYLAEKVSFAAMEMIRNEGIDVVIDLHEAEAMYPV
                     TNCIVAPEKSMPYAIGAAFYVKGREKFENHVESSPAGYRGLSHREIGDWSDAYPFLLE
                     SPGVHLDQITAAKTPELIIDGIDPFVLKAGEKGLLFVPYDENGFSMDRRVGQHSSVIQ
                     EILSQWTKKNPDRGFKVSAPKHADIVENGLGFYFKDPSEADENKVFLQ"
     gene            complement(5490..6116)
                     /locus_tag="PKL93_10500"
     CDS             complement(5490..6116)
                     /locus_tag="PKL93_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNQ71727.1"
                     /translation="MIENLIWDFDGSLFNTYPAMVRLFKQSLAIHGYGASEDEILSLM
                     KETLGKAVDFYLDRGVDYAFYKDFQRMEEELAPVEQPPFSGAREVCSRIKRDGGVNLI
                     ITHRARRTAFKLLDYFEMTSLFSEIVTKESGFKRKPDPDAFISAIRRHRLKPELTLSI
                     GDRDLDVMAARDAGTKTCFFSQDGSKPSIDVDYIVDDLLQLGRIIQEE"
     gene            complement(6109..>6420)
                     /locus_tag="PKL93_10505"
     CDS             complement(6109..>6420)
                     /locus_tag="PKL93_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNQ71728.1"
                     /translation="NYYNMLDLFSEIVSADSGFARKPDPQAIMYIIDKYGLERERVLT
                     IGDREIDIEAGKRAGIATCLFNPDKDFVKTSADFVLTSLSQVPSVLQVCNRFSGCEGD
                     D"
BASE COUNT         1634 a         1380 c         1627 g         1779 t
ORIGIN      
        1 gatattgaca ttgaagatcc gctcgggatg acagatgggt ggataatcgt tgttaatgaa
       61 aacggtgagc ttgtcgaatc gttgtcggta ggtggctcgg ctgacgattt cattctttcg
      121 ggtgtcaatt acggtgaaaa gtacatttta tgtggttgtt caacgtcatc tgacggagac
      181 ctggaaagca acaagggtaa aaaagacatc tggttgatta cgttaggaaa atagataagg
      241 tctagcgacc ttaaactggg gttaatcccc aataaaatca agttgggagg ggtttcaatt
      301 gcgtaagatc ctgttggtcc tgattgtggc tgcagcaata gttagcatcg gctttgcatg
      361 taccacgatc atcgtaacta agggtgcgtc tgttgacggt tcggtaatga cgagtcattc
      421 agctgactgc ggcctctgtg atttcaggta cgtctatgtt ccacccgcag actatgaagc
      481 gggagcaaag agagcagttt atccattcgt tgagccatat ccacgttatg tcggtgcgga
      541 tatgggtccg acctacaacg atccggacct tccggctact gagcctctgg gttacatcga
      601 tcaggtcgag cacacatatg gctactttga tgcagtctat ggtgtgatca acgagcacca
      661 gcttgccatt ggcgaatgta cttgttccgc aaaggtctat gctcagccaa aggccggaga
      721 atgcatattt gacgtagccg ctctttccag agttgctatg gagagatgta caaccgcaag
      781 agaagccgtt gaactgatgg gcgcacttgc cgttgagtac ggttactacg gttggggaga
      841 aacactcaca gtaacagacc ctaatgaagc atgggtcttc gaaatctgtg caacgcctga
      901 caagaagagc gcactttggg cggcaaagaa ggttccagat ggagaagtct tcgtggaatc
      961 aaacatgttc aggataagag aactcgatcc tgaaagccct gacaatatgt tctcaccgaa
     1021 tcttatcgat gttgcaacag aagccggctg gtacgatccc agcacaggtc ctatcgactg
     1081 gatggcaact gtaagcacag gcgagtactc catgccttac tacagtctta gaagaacatg
     1141 gagagttctc gacagagtct ccccatctct tggactttct ccctgggtag aagacagttt
     1201 tacaaaagac tacccattct ctatcgtgcc tgacaagaaa ctgtctgtgg ccgatgttat
     1261 cgacctcttc agggatcatt accagggaac agagttcgat ttgactgaag gacttgccgc
     1321 aggacctttt ggcaatccca atcgctatgc aggttcaagc aaactaataa aggggagctg
     1381 ggaaagagct ctttctatct tcagatgtga atacgtattt gtgtctcagg tcagagattg
     1441 gcttccggat cctgtcggcg gcgtagtatg gtggggcgcg gctgctcctc acgagacaat
     1501 tctagtgccc atgtactgtg gaataacgga cgttccttat gcttatgaca gtggtagcct
     1561 tcaggaattc gactacgatg tcgcatcatg ggcattcaac ttcatgggta actgggcgga
     1621 gttgaagtgg agctacatgt acccggagat tcaggaactg cagaagaaga tcgaaggaaa
     1681 gctctttgca gtacagccag cgatcgaagc ggcagctgca cagctttacg aaacagatcc
     1741 agaactgtgt aaggaattcc ttactgatta cgttgccgac gtcactgaca gagtaatggc
     1801 agaagtgtgg gatttcaacg aataccttat tacgaagtac agagacggat acataaacat
     1861 tccgaacgtt ggttcgtctg caggataccc cgactggtgg cttgatgcag taggatacga
     1921 cgaaggccat attttcggcg aagacggcta caaagctaag taatcgactt acccgccccc
     1981 aaagtggggc gggttttgct ctttcttgta tttcaaacct tgtagacgat tcttgattca
     2041 tatatagtgt tgaagatttc gaatgaggag gttactaaca tgagaagatt tttgttagtt
     2101 ctgattgtag tttctcttgt cagtgttttt gcgtttgccg aacccgaagt aaaactcggt
     2161 ttgcctgtcg taataacttc tgcaggtcag agcgcagagg ttgaaacagt caactatgtt
     2221 gccgatgaag tcggcctaaa gtatgattac tgtgacgttc tttccaagga tgaacttgct
     2281 gctggagttg gactcggtgg agccaagtca gcaataggaa agcacgttac gacgctttct
     2341 cccgatcctg aaggtactaa gttccagtct ctgttactcg ttcttggcgc cagccttaaa
     2401 ggcatgggtg cttccggtct ctctgttgaa actgaagttg acagactcaa ggaccttata
     2461 gaatatgcga aggcaaacaa aataaagatt attgcagttg ctattggagg agagattcaa
     2521 agaggtcttc caggaagtcc gatcgaagtt attatcgaca ccgtcgttcc ccatgctgac
     2581 gtattcatag ttacaaagga ttcaaatggc gatgggaagt tcacgaaggc ggcgcaagcc
     2641 agaaacgttc cgatagtaga gatcgattct gcatttgatc ttctcgatac ttttacacag
     2701 gtatttggct tgtagtttct tggtggccgg cgcaatagtt ttatctaccg cgccggctcc
     2761 aattaaaggg ggtaatcaga ttgtcagctg atgcaatata catgcaaacg atcatagcag
     2821 gaatagttat ggttggagcc tatgccgtcg ggaagtttct gaaattttca accgaactat
     2881 gtatgtttgt cgcagtaatc ggtggtgcca ttgccggggg agcgggattt gatactttca
     2941 gacatattgc cgaaggatcg gtaacctacc tcgacattgg actaattttc gtgtttgcaa
     3001 cgatatttat gaacatcatc aaggaatcgg gtggaacgaa ttacatagta agaaagataa
     3061 taggtgcctt ccacaacaag agaatactga tgctgatact tctcatgctt gtgattcttg
     3121 ttccaggagc cctaacaggt gccggaagca tctcggtctt ggttgttgga ggaacggttg
     3181 cggccgctct caacgcaatg ggactttcga aggtgagaat ctcggcgttg attttcattg
     3241 tcgcgggttt gagtgccgct gctccgccag ttaacatctg ggcgatgatg acctgtgccg
     3301 gaacggccat tccttacgtt ggatttacga tgcctcttct cataccggtc ctaattcttg
     3361 ggcttttcac cgtgctgttt tttgggcgcg gggcaggaaa tatcgacaaa gaaaaggctc
     3421 tcaaagagat tcccgatcca aaaggactct ccggatggag tgttggagta cctttccttg
     3481 tcttgatctt cctgcttgga gctcctagaa tctggccatt tgatttcccg gtcctcggat
     3541 tgcctctggt atttgctatc tgtgctttgg tagcatggct tatgaacttc aagagagtca
     3601 atatattgaa ggtcagcaga gaaacggtcg ctcagttgac gccgctcata gcgactaccg
     3661 ctgtcgtcgg tatgctgata caggtgatga gttttaacgg tgtcaaaggc ttgatttcca
     3721 tgtggattgt gactgcccct ctcgcgatcg tctggattct ccttcctttc ataataccaa
     3781 tatcggaagg tctcctgacg tatggtggtg ctatggtaat tggcatacct cttatatgga
     3841 tgttgaactc gaacgggatc aacccggtaa tcgttttggc aggactgagt ttactgtggc
     3901 ctcttgggga tggattgccc ccgactgcgc ttattgggcg cttgacggtt agcacagtag
     3961 gttacaaagg ctcatatggc tcattcttga aagaatgcgt ggtgccctgg atagccataa
     4021 ctgtcgttgc aatgatactg gttatctttg caaataaatt caacttcctg atgatggtag
     4081 gttgaggagg aggtgtcgtc atgactttga taatgtttct gtattatgtg atgacagcct
     4141 atgtggtagg catgcttatc tggaacttca taaagacgaa ggatataaac agcgaaatct
     4201 tatatgcact ggctctcttg cccctcattc tcagattgct tagaatgagg taggagggat
     4261 attatgatga gaaaactatt gatactggtt gttctggcac ttattgtctt ggcgggcgga
     4321 attcctttgt acatacagag gagttacaag gaagaagtag tcgccgggcc gagcgtgaca
     4381 gacgtgttca agctaagcaa gtatttcgac ggaatcaaag gaactattgc cgataccgac
     4441 gtcttcgagc ttaaaggagc agaagagggt ggaaagacgt tgataatcgc gggaacccat
     4501 gcaaatgaac cctccgctgc cttactagcc tatttcttca ttgagaatct tgaagtggaa
     4561 aagggaacag tatatattat tccgcatttc aacctgagtg gttctctggg gccgcagccc
     4621 ggtggcggtt tcccactcta ttacttcgtg gatactccct ggggcgaaaa gaaattcaga
     4681 atgggcgatc gaggcttcca ggcactcgac cagtggcccg atccagatgt atatgttcac
     4741 tatccagacg gccagttgtt gtcctatatc gatgcaagaa acacaaacag atcttggccg
     4801 ggaagacccg atggctacct tgcggaaaag gtttcctttg cagcaatgga gatgatccgt
     4861 aatgaaggaa ttgacgtggt tatcgatctt catgaggctg aggcaatgta tccagtaaca
     4921 aactgtatag ttgctcccga gaagtcaatg ccctacgcga taggagctgc gttctatgta
     4981 aagggaaggg agaaattcga aaaccatgtg gaatcttcac cggcgggtta caggggtctt
     5041 tctcatagag agatcgggga ttggtcagac gcatacccct tcttactgga atctcctggt
     5101 gtgcacttag atcagattac ggcagcgaaa actcctgagc tgatcataga cggaatagac
     5161 ccgtttgtac tgaaggccgg cgaaaaggga ctgcttttcg ttccatatga cgaaaacgga
     5221 ttctctatgg acagaagagt tggacagcat tcttctgtca tacaggagat tttgtctcag
     5281 tggacgaaga agaatccaga tcgcggcttc aaagtctcgg ctccgaaaca tgcggacatt
     5341 gttgaaaatg gtctcgggtt ttatttcaaa gatccttctg aagcagatga aaataaggtc
     5401 tttctccagt aggattttgt ctagggtgaa cgagtgcatg cgttatcgag gacgcatgca
     5461 cttttttagt tttcgtcacg gtttcgttat tattcttctt gaataatccg ccccagctgc
     5521 agcagatcgt cgactatgta gtcgacgtct atcgaaggtt tcgacccatc ttgcgaaaag
     5581 aagcaggttt ttgttcccgc atcccttgcg gccatcacat cgagatctct gtcgccaatg
     5641 gagagggtta attctggttt taatctgtgt cttctaatcg cgcttatgaa agcatctgga
     5701 tcgggctttc ttttgaatcc gctttctttt gtgacaattt cactgaataa ggaggtcatc
     5761 tcaaagtaat ctagcagttt gaaggctgtc cttctagctc gatgagtaat tattagattc
     5821 actccaccat ctcttttaat tctcgagcat acctcacggg caccactgaa tggaggctgt
     5881 tcgactggag ccaattcttc ctccattctt tggaagtctt tgtagaaagc gtaatcaact
     5941 ccacgatcta gataaaaatc tacggccttt cccagcgttt ctttcatcag cgagagtatc
     6001 tcatcttcag aagcaccgta tccgtgaatt gccagagact gcttgaatag tcttaccatg
     6061 gcgggatagg tgttaaataa cgacccgtcg aaatcccata ttagattttc aatcatcgcc
     6121 ttcacagccg ctgaatctgt tgcaaacctg gaggacagaa ggaacctggc ttaaagaggt
     6181 taagacgaag tccgcgctgg tctttacgaa atccttatct ggattgaaaa ggcacgtcgc
     6241 tattcccgct ctctttccag cctctatatc gatctctctg tcacctatcg tcaagactct
     6301 ctctctctca aggccgtatt tgtcaattat gtacattatt gcttgaggat caggctttct
     6361 cgcaaagccg ctatccgcag atacgatttc ggagaagaga tcgagcatat tgtagtaatt
//
LOCUS       DAOQYI010000078         6318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_12692_length_6318_cov_62.339773, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000078 DAOQYI010000000
VERSION     DAOQYI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12692_length_6318_cov_62.339773"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1544
                     /locus_tag="PKL93_10510"
     CDS             99..1544
                     /locus_tag="PKL93_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ71729.1"
                     /translation="MGIVESMKTSMLRQAGKYVGSVVRNSSTENIARLFGTVAALTKE
                     PTKSGLKKLTQMAKEEHPMIVSWQNVFKRSSPKAVEKAMTNLVVNEFALGEKIRQEKM
                     LEHEVVIPKLVVISPTYACNLNCVGCYAGLYGRKYQLSKEEVSSIIRQSNELGIYFFI
                     ITGGEPFVWPHLLEIFEEFNDSYFQVYTNGTLITKEVAQKLAELGNATFAVSVEGFEG
                     ETDWRRGRNVFQKIKQAWANLREAGVIYGTSVTATRLNHEVMMKDEFWQFLKDEGVSY
                     AWVFQFMPVGMNPSMDLVPTPEQRYERFYKTEQERLGGDFAFVADFWNHGFLTNGCLA
                     AGAKYLHVNAKGYVEPCVFQQFAVDSIREKSLLEVIKSPFFTAYKRMVPYSNNLFRPC
                     PIIDNPKVLRAMVNKFHAIPQHDGSENVVTELAGQLDELAEGWKKYADKLYYEEGFAE
                     THPAHRGVYDFDVRMRKYANNEEKLAIDKKP"
     gene            1561..2130
                     /locus_tag="PKL93_10515"
     CDS             1561..2130
                     /locus_tag="PKL93_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ71730.1"
                     /translation="MRAKLSSRERIIRAARKAFAERGHDGVSMSEIAQMAGVKKALIY
                     YYFPSKEDLFYEVWQYSIDELEDHVFSETSDENVYVSKLKRILRSYIDFITSKNEIKK
                     IMDQERANLSREDNESWKRVRDRYEALKEKLSSAINDAKAAQEISKDVDPDSAADLIL
                     NGFSITEDPDGLESIREIIWRGLVSNSGE"
     gene            complement(2153..>2611)
                     /locus_tag="PKL93_10520"
     CDS             complement(2153..>2611)
                     /locus_tag="PKL93_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ71731.1"
                     /translation="YAFGFDAVAGHDLYLKMLQSGKRVKLIENGYEQMISAYNLKDDS
                     VVVAISSTGTSVDLLDALKFSEKTGASVITVTPAGSRLSTYSNVNLESYYSKLIFPEG
                     GLITRIVQLMILDVLYLKFLQISGKNFEEKYSKFREVLDFKRRGTKKERD"
     assembly_gap    2612..2711
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2712..3153)
                     /locus_tag="PKL93_10525"
     CDS             complement(<2712..3153)
                     /locus_tag="PKL93_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNQ71732.1"
                     /translation="MSSLIQSYNEALGLFTQTEEKIASFIMTNPEAALELSVNDLASR
                     LNVSPSMVVKASQKMGFTGYSQLKLAVASELNVLVQREKSTILIEDLDAYEDLVSSTI
                     REAYSRLGEDDLKKAAELLVESENIDIYAFGFDAVAGHDLYLKML"
     gene            complement(3146..4138)
                     /locus_tag="PKL93_10530"
     CDS             complement(3146..4138)
                     /locus_tag="PKL93_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ71733.1"
                     /translation="MRRAILLFSLLLAVTFAASKFSVGILITGEIGGNAIYELVQRGA
                     LEMASEEIEIKIVEGGYNQSKWEQMLISLAALGRYDLLMTFTEGMPASVNKVAEMFPN
                     QKFALLDGIIENPLPNTYSVAFKDDEMTFLAGIFAALITSSSLPGANEEKVVGLIAGD
                     TYPAMTNVMRPSFENGVKTVHPSIRVFFGVVGSWSDPARGSELAAKQFDAGADVILSI
                     AGGSGIGVIEEASVRGKYVITVDSNMIALNPSVILGSFLKHIDVLVKNTIADAKAGTL
                     PFGTSKRVGIAEGMIDYTHDDPNFVRNVPDDIRAQLAEWFEKIKAEGLPKINNE"
     gene            complement(4227..5303)
                     /locus_tag="PKL93_10535"
     CDS             complement(4227..5303)
                     /locus_tag="PKL93_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71734.1"
                     /translation="MRRNYFRFLFLMIFIFIAISSLAGSSDSTDSADATVTTYFEDVA
                     REILAMDSFDDLFSLDGFFEDDPAAELTPSHPIPPTPVSTVSHQDPAMPSITPQATQS
                     ETESPESTLTWKYEPYADSLLPGGIAFEFEAGDPVMGIPDVVKFYKEGVVVDTLAGKT
                     IMSPVIVSDVPFPGQKTTIFRFLTDDYSTPLGGYYITVVDSEVYSLTVALDPTRAENI
                     VDLDCDGIVELIVVDDHMIKAQEETELSVFKRFFTWTEDGWQVDSPGDYVNVYSLMAE
                     ADLEKIEASSPKTPHVWSYAYHSYMAGEDLEEIQFRVAKLVEDFSLNWLINSTEMVKL
                     LDQNINQYNGYVMKRPTPDSSPTM"
BASE COUNT         1661 a         1455 c         1470 g         1632 t
ORIGIN      
        1 aatgttgcat aatcttgttg taaatgacat atagatgtag tattgaagga ctagtatttt
       61 accgaccggt aagtaaaaaa ctattggggg tgttattcat gggtattgta gaaagtatga
      121 aaacttccat gctaagacag gcagggaagt atgttggttc agtcgtcagg aattcttcta
      181 ctgagaatat tgcaaggtta tttggcactg ttgctgcttt gacgaaagaa ccgacaaaga
      241 gcggtctaaa gaaactgacg cagatggcca aggaagagca tccaatgatc gtgtcctggc
      301 aaaatgtttt caagagatct agccctaagg cagtagaaaa ggctatgacg aacttggttg
      361 ttaacgaatt tgctctcgga gagaagattc gtcaggaaaa gatgcttgag catgaggtcg
      421 tcattcccaa gctagttgtc ataagcccga cctacgcctg caatttgaac tgcgtgggtt
      481 gctatgccgg tctctacgga agaaagtatc agctttcgaa ggaagaggtc agttcaatta
      541 tcagacagtc aaacgagctg ggcatttact tcttcataat taccggcgga gagccttttg
      601 tatggccgca tcttctcgag atttttgaag agttcaacga ttcttacttc caggtctaca
      661 ccaatggtac tcttatcacg aaggaagtcg cccagaaact cgccgaacta ggtaatgcga
      721 cgtttgccgt ctcggtagaa ggctttgaag gcgaaaccga ctggagacgt ggaagaaatg
      781 tcttccagaa gataaagcag gcctgggcaa atctgcgaga ggcaggagta atttacggta
      841 cctctgttac tgcgaccagg ctgaaccacg aggtaatgat gaaggacgag ttctggcagt
      901 tcctgaagga cgagggtgtt tcttacgcct gggtcttcca gttcatgcct gttggtatga
      961 atccctccat ggatcttgtt cctactccgg agcagagata cgagagattc tacaagaccg
     1021 agcaagagag gctcggtggt gacttcgcat tcgttgcaga cttctggaat catggattcc
     1081 ttacgaatgg ttgtctggcc gccggcgcga agtaccttca cgtcaatgca aagggttacg
     1141 tagaaccatg tgtattccag cagtttgccg ttgatagcat cagggagaag agtcttctcg
     1201 aagttatcaa gtcaccgttc tttaccgcgt acaagagaat ggtgccctac agcaacaatc
     1261 ttttcagacc ctgtccaatc attgacaacc cgaaggtcct aagggcaatg gtcaacaagt
     1321 tccatgccat tcctcagcat gatggaagcg agaacgttgt tacagagctt gccggtcagc
     1381 tagacgaact cgccgaaggc tggaagaaat acgcagacaa gctatactac gaggaaggtt
     1441 ttgccgaaac ccatcccgcg cacagaggcg tgtacgattt tgatgttaga atgagaaagt
     1501 acgctaacaa cgaagaaaag cttgctatag acaaaaaacc ttgaggggat ggtggatccg
     1561 gtgcgagcaa agttatcttc tagagagagg attataagag ccgcccggaa ggcctttgcg
     1621 gagagaggtc acgacggcgt cagcatgtct gagattgcgc agatggctgg agtgaaaaag
     1681 gcacttattt actactactt tccgagcaaa gaggatcttt tttacgaagt ctggcaatac
     1741 tctattgacg agctcgaaga tcatgtcttt tccgaaacaa gcgacgagaa cgtttatgtc
     1801 tcaaagctca agaggattct gagatcgtac atagatttca taacgagcaa aaatgagatc
     1861 aagaaaatca tggatcagga acgtgcgaac cttagcaggg aagacaacga gtcctggaag
     1921 agagtgagag acagatacga agccttgaaa gagaagttgt cgtcggccat aaatgatgca
     1981 aaggctgctc aggaaatttc gaaggacgtc gatccagata gcgcagcaga cctgatattg
     2041 aatggtttca gtataacgga agaccctgac gggctagaaa gcataagaga aataatctgg
     2101 aggggcctgg ttagcaactc cggagagtaa ttaagaaaga agagagaggg actcagtccc
     2161 tctctttttt tgtgcctctt cgcttgaagt cgagaacttc tctgaacttc gaatactttt
     2221 cctcgaagtt cttcccagaa atctgaagaa acttcaggta aagcacgtcc aggatcatca
     2281 gctgcactat acgagttata agaccaccct ccgggaagat cagctttgag taatagcttt
     2341 cgagattcac gttcgaataa gtgctcagcc ttgatcccgc cggtgttacc gtgattaccg
     2401 aagctcccgt cttctcggaa aacttcagcg cgtctaacaa gtctaccgaa gttccagtgc
     2461 tggatatagc aacaacgaca gaatcatctt tcaaattgta tgcagatatc atctgttcgt
     2521 atccgttctc gatgagcttg actcttttgc cgctttgcag catcttcagg tagagatcgt
     2581 gtcccgctac tgcatcgaaa ccgaacgcgt annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn ngcagcatct tcaggtagag atcgtgtccc gctactgcat cgaaaccgaa
     2761 cgcgtagatg tctatatttt cgctctcgac cagaagctct gccgccttct ttaagtcgtc
     2821 ttcgccaaga cgggaatagg cttcacgaat tgttgaggat actaggtcct cgtaggcgtc
     2881 caggtcctcg atcagaatgg ttgacttttc ccgctgcaca agcacgttaa gctcgctggc
     2941 gacagctaat ttcagctgcg aatagcctgt gaaacccatt ttctgtgaag ccttcacgac
     3001 cattgacgga gacacattca accttgaggc aagatcgttc actgaaagct ctagagccgc
     3061 ttctggattt gtcatgatga agctggctat cttctcttcc gtctgagtga atagtcctaa
     3121 ggcttcattg taggactgta taaggctact cattattgat ctttggcagt ccttccgcct
     3181 ttattttttc gaaccattcg gcaagttggg cacgaatgtc atccggaaca tttctcacga
     3241 aatttggatc atcgtgagta taatcgatca ttccttctgc tattccaacc ctcttggaag
     3301 taccgaaggg aagtgtgccg gctttggcat ctgcgattgt attcttcacc aaaacgtcta
     3361 tgtgcttcag gaaactccca agaataacgg agggattcaa agcgatcatg ttagagtcga
     3421 ccgtgatcac atatttgcct ctgaccgagg cctcttcaat gactcctatg ccactgccac
     3481 cggcaatgga gagaataacg tctgctccgg catcgaactg cttcgcagcg agttcgctcc
     3541 ctctagctgg atccgaccag ctaccaacaa caccgaaaaa cactcgaatc gaaggatgaa
     3601 ccgtctttac gccattctcg aaagagggtc tcatgacatt ggtcatcgcg gggtaagtgt
     3661 cccctgcgat caaccccacc actttctctt catttgctcc tggcaaactg cttgaagtta
     3721 tcagtgctgc gaagattccg gcaagaaagg tcatttcgtc gtctttgaaa gcaacagaat
     3781 aagtgtttgg aagagggttc tcgataatcc cgtccagtaa tgcgaacttc tggtttggga
     3841 acatttcggc gactttattt acgcttgccg gcataccttc ggtgaaggtc atgagaagat
     3901 cgtacctacc caatgccgca aggctgataa gcatttgctc ccacttggac tggttgtatc
     3961 cgccttcgac gatctttatc tctatctcct cagaggccat ttcaagcgcg cccctctgta
     4021 caagttcgta aatggcgttt ccaccaatct ctcccgtgat aagaatccct acgctgaact
     4081 tcgaagcggc aaatgtaacc gccaatagca gggaaaaaag aagtattgct cttcgcatct
     4141 cttcacgctc ctctagcttc attcgtttcg attatagttc tttcgaagga aattctcaag
     4201 agcgcgaatt atattcttga tttggattac attgtaggtg aagaatcagg ggtaggcctc
     4261 ttcatgacat aaccattgta ctgattgatg ttttgatcga gaagttttac catttccgtg
     4321 gagttgataa gccagttcag ggagaagtcc tcgacgagtt ttgctacacg gaattgaatc
     4381 tcttcaagat cctcacccgc catgtatgag tgataggcat atgaccacac gtgaggcgtc
     4441 ttcggactgg aagcttcaat cttttcgaga tcagcctcag ccatcaaaga atagacattt
     4501 acgtaatcac caggagaatc gacctgccag ccatcttctg tccaggtgaa gaatctcttg
     4561 aataccgaaa gctcggtctc ttcctgagcc tttatcatat gatcgtcaac gactatcagt
     4621 tctactatcc catcgcagtc gagatcgacg atattctctg cgcgagtggg gtcgagcgct
     4681 acggtcaagg agtagacctc tgagtcgact acagtaatgt agtatccacc caaaggagtc
     4741 gagtaatcgt ctgtgaggaa cctgaagatc gtggtcttct gtcctgggaa gggaacgtca
     4801 ctaactataa ccggcgacat gatcgttttt cctgccaggg tatctacaac gacaccctct
     4861 ttgtagaact tcaccacatc cgggattccc attaccggat cgccagcctc aaactcaaat
     4921 gcgattcctc caggcagaag gctgtccgcg taaggctcat atttccaggt caatgtgctt
     4981 tcaggtgatt ccgtttcaga ttgcgtggcc tgaggtgtaa ttgaaggcat ggctggatcc
     5041 tggtgtgata ctgtgctcac cggagttggg ggaatcggat gcgaaggagt cagttcggct
     5101 gcgggatcgt cttcaaagaa gccgtcaagg gaaaagaggt cgtcgaaaga atccatggcg
     5161 agaatctcgc gcgcaacatc ttcgaagtaa gtagttacgg tagcatcagc agagtctgta
     5221 gaatcgctgc tccccgccag acttgaaatc gctataaaga tgaaaatcat caaaaacaag
     5281 aaacgaaagt aattccttct catactgacc aatctcccac atcgtaatta tacgctattt
     5341 tataatgcgt tagcccagct gactttcgct aactcgggaa cttgttcagc aagtatcttg
     5401 tttcattgtt tctgtctagg ctttatgaag ttcatcactt agtcatcacg aactcgtttc
     5461 gggatctaca atcctatgga gaaatgtctc tcaaatcacc gatcgagtag ctcattattt
     5521 gcatcaacat cttctctgat acttagtgat catgctcctt agttccagaa ttggttagaa
     5581 ttctcatgta gcctctgtct cgatatgtca cttattctct gtagctcttt tacgaaaaac
     5641 aaaggaactg gacgcgtaac gttggaacta taggctgctt ttctctcttg cgaaacccta
     5701 caccccacag ccctagaccc atttcctcct ggctcttgat cctaaatccc cgcttgagcg
     5761 ggggggaccg cttgcggtgg ggtgtgtatg ctcttaagcg cattgccaaa cgtgtgcctt
     5821 ctttaacacc gaaaggtatt acttttgtag gggacgcgtt agtatctcca ttacttctgc
     5881 cttaactaac gcaccaatat tcgtacgagt tttcttgtct taagatactc acaccctccg
     5941 ccactacgtg gccctcctcc ctcaagggag gatttaagat caggatcatt aggagccaaa
     6001 tacaaattcg cgcaagaacc atcgaagggt aaggttttaa tgaatgacgt tgagagtctt
     6061 cttcagcgaa aagctgatct tttgccgtgg agctgaagtg ggctgcgtta gcagactggc
     6121 tgattgttgc ccaaaatcca ctcactctat catcccgtag tgctcctgta cgggatctcg
     6181 catcaaagaa tcgctgtacg gttaagaacg aaaacccgct gaacgcttaa aaaacccgtt
     6241 caccgtcacc ggtcctccgt cctccgcata tttaccccgc aaggatattg gagataaata
     6301 gaagaacaga taggatag
//
LOCUS       DAOQYI010000079         6124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_13151_length_6124_cov_79.609326, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000079 DAOQYI010000000
VERSION     DAOQYI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13151_length_6124_cov_79.609326"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKL93_10540"
     CDS             <1..306
                     /locus_tag="PKL93_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ71735.1"
                     /translation="PVAVCIDYIPKSIVKDIDPDRIHNSVFEGLGKHYNIDIRYAECD
                     LIPSSCDEVLSERLKVDVGTSLLLLEQIHYDVLDRKVLYSKSYFPSGKFTFKLIRRR"
     gene            337..1581
                     /locus_tag="PKL93_10545"
     CDS             337..1581
                     /locus_tag="PKL93_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71736.1"
                     /translation="MRKLLLVTVLLLGVMLSATTIEVLWMGWPQDQVMQLVNAFKEET
                     GIDVDIQLVPFGQLFQTIEVRLAAGDGTPDVYIVDGPNTASYAARGYLLPLDEHFSDE
                     EMSAWFDASIEEGSYQGAFYSVPYGTSSAGIFYNKAIFEEYGVPFLPETIEERLTWEE
                     VAEIAKKLTKDTNGDGLTDIWGLVIEQIDRPYLIFPMVQSLGAKVLSDDGLESQGYIT
                     SDEFVEGTTFYWKLFNEWKVSPQGLNDSAISREYFGTGRAAMMLGNEWNLRRMLQYPD
                     VDYGLSPFPYFEGGVAVTPTGSWHVGINSKTKKLEEALAFTKYITGKEAVITWHKLNG
                     IAPARPDVYEALPEVFDNPMWQLFVKEMETTAVPRPRTPGYSQYELMLREAFNSIHYG
                     ADPRSTLEEVAVRIDRELRKYR"
     gene            1630..2496
                     /locus_tag="PKL93_10550"
     CDS             1630..2496
                     /locus_tag="PKL93_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ71737.1"
                     /translation="MFRNNKVAWLFVLPALVFLLVFKIWPIGVSVIESLTMTSYTGTK
                     SFVGFENYEYLFKHDPVFWKSFSVTLLYSIIVNPLIVLTSLLMALLLNSSHFFTKFFR
                     TVFFLPTAISFAVVSVIWMIVLDPYNGLANSFLTMFGFKPQPFFASPDQALWGLILLN
                     VWRSSGYWMMYFLAGMQNIPETLYEAAEIDGASTLSKTFKITLPLLTRTISFVLVANT
                     AFNFLSFAPVYIITKGGPMGSTNLLMYESYKSAFVNLDMGRASAITTILLGIILVFSF
                     FELRFTRARFEY"
     gene            2506..3348
                     /locus_tag="PKL93_10555"
     CDS             2506..3348
                     /locus_tag="PKL93_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ71738.1"
                     /translation="MKKRSLWKTLILFAVLGIISLFFLIPLIWVVASALRPASPLYEY
                     ANPLTWKSFIPTQPTLENFVHIFVNLNFGRALMNSLFVSVSTIVLTVLVASMAGFALA
                     KFEFRGKAALFTIVLITFMVPFESIVIPLYILIKQLHIDNTYWALILPGVANGLAIFL
                     FRQFFSEVPSEIMEAARIDGASWFRIYWRIVLPLSVPAIVTVIVMVFMFQWNSLFWPL
                     VATHSSRFEVVQVAIAAHRSTENTSWANLFSSAIAGSLPPVILFLFLQKYFVRGISGT
                     GLKG"
     gene            3353..4165
                     /locus_tag="PKL93_10560"
     CDS             3353..4165
                     /locus_tag="PKL93_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNQ71739.1"
                     /translation="MNNSKKAKNHLQISNDSLPEIALLPGDPARVYEIGECLSNVEEI
                     GNNRDYVTVTGTYKGLPLTVCSSGIGGPSTEIAVVELNRLGVKTIIRVGTSGGLADDV
                     KPGDLIVLSSCIRYSGTANLFIPENFPAVADYRLLTALISACEEAGVVYHVGIGLSLD
                     SFYATKPDLLREDFPSSIYGKLEEWIAAGALQLDMEAATLYVLSSLLKINAAAICTAG
                     SNISRGERPETPPSNKNAIRAACEAAYKFNNWKKISVKRGRNFTLPPIAERG"
     assembly_gap    4250..4349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4350..4706
                     /locus_tag="PKL93_10565"
     CDS             <4350..4706
                     /locus_tag="PKL93_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ71740.1"
                     /translation="GKIINLASVGGLFPEKEHAGHCAAKAGIIAISKVMALELAPANI
                     QVNVIAPGPTETVPFTSPFYSQHPEVLKRIEEKTPAGRIGHPEDHTGLMVFLASDESN
                     WITGQVIMSDGGLGLV"
     gene            complement(4778..5962)
                     /locus_tag="PKL93_10570"
     CDS             complement(4778..5962)
                     /locus_tag="PKL93_10570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71741.1"
                     /translation="MFECRRISNQKDYLDLMKFSFGITENWMRVASKHAGEIFNNDLR
                     DPFGAYDGSTLAAEYLLLSLKMRLRDSVIPMGGIGNVCTSPLYRGKGAVKFLLEKSLE
                     TMREKGQAVSLLYPFSRSFYRKLGWEEFDTMLRAKFSPGSIDLPDQKVEVKIEEMEDA
                     DGEIREFYNEYASSHYSMILRDEEMWKSDFEFRTDNDVSKKFVKFKREGRTTGMLRYV
                     FLYDKFDKDSGLKFVVTIFLADDVETRHAMFRFLKGLSLQIDQIHAFLPPDFLLWPYL
                     KERPSEMKIVPRTMIRIVDLQLLNGLRIDAPDIRVGIKIDDSQASWNDGVFELCVENG
                     ELGFSPSDSFDLECDISTLSSVVGGSTNFKEMIEFGRVKVSDGYKGQDLPKSLPFELQ
                     HF"
BASE COUNT         1526 a         1481 c         1456 g         1561 t
ORIGIN      
        1 cccgttgccg tttgcattga ttacattccg aagtccatag tgaaagatat agatcccgac
       61 agaattcaca attctgtctt tgagggactg ggtaaacact ataatatcga tatcagatat
      121 gccgagtgcg atttaattcc ttcaagttgt gacgaagtcc tatctgaaag gctcaaggtc
      181 gacgtgggta cttcacttct cttactggag cagattcatt acgacgtttt ggatagaaag
      241 gttctgtatt cgaagagtta ctttccgtcg ggaaaattca ctttcaagct catcagaaga
      301 cgttgagata gatagctaaa caggaggtgt agggaaatga gaaagctttt actggtgacg
      361 gttctattac tgggtgttat gctttcagcc acaaccattg aggtcctatg gatggggtgg
      421 ccccaggatc aggttatgca gctcgtcaat gcgttcaaag aagagacagg aatcgacgtg
      481 gatattcaac tggtcccctt cggccagctt ttccagacga tcgaagtcag acttgcggct
      541 ggcgatggta caccggatgt gtatattgtg gatggtccaa acacggcctc ttatgcggca
      601 cgaggctatc tccttcccct ggatgaacac ttcagcgatg aggagatgtc tgcgtggttc
      661 gatgcctcta tagaagaggg cagctatcag ggcgctttct attcggttcc ttacggaact
      721 tcatcggccg gcatattcta caataaagct atcttcgagg aatacggtgt tccatttctg
      781 ccggagacaa tagaagagag acttacgtgg gaagaggtgg ccgaaattgc gaagaaactc
      841 accaaagata ccaacggtga cggacttaca gatatctggg gtcttgtgat tgaacagata
      901 gacagaccgt atctgatctt ccctatggtt cagtcactag gcgcgaaagt cctttctgac
      961 gacggtcttg aatctcaggg atatatcact tcagatgagt tcgttgaagg aaccaccttc
     1021 tactggaaac tcttcaacga atggaaggtc agtccccaag ggctcaacga ctcggccatt
     1081 tcacgagagt atttcggaac tggcagagct gcaatgatgc ttggaaacga gtggaacttg
     1141 cgaaggatgc tccagtatcc cgacgtggat tatggacttt cccccttccc ctatttcgag
     1201 ggcggagttg cagtcacccc aacaggcagc tggcatgttg gaattaactc caagacaaag
     1261 aagcttgagg aggctctggc cttcacaaag tatattaccg gcaaggaagc tgtaatcacc
     1321 tggcacaagc tgaacggtat tgcaccggca aggccagatg tttacgaggc ccttcccgag
     1381 gttttcgata acccaatgtg gcagctcttc gttaaagaaa tggagaccac cgcagtcccg
     1441 agacctagaa cacccggata ctctcagtac gagctaatgc tgcgggaggc attcaactct
     1501 attcactacg gtgccgatcc aagaagcact ctcgaagagg tcgccgtacg aatcgacaga
     1561 gaacttagaa agtacagata gttttccagg gatcgtgctt gaatttagat tattccggat
     1621 ggtgtgcaga tgtttcgaaa taacaaagta gcgtggcttt ttgttctgcc cgcgctggta
     1681 tttcttcttg tattcaagat ctggccaata ggcgtctcgg ttattgagag ccttactatg
     1741 acgagttaca ccgggacaaa gtcattcgta gggttcgaaa actatgaata cctcttcaag
     1801 cacgatcccg ttttctggaa atccttttct gttactctct tgtactcaat aatcgttaat
     1861 cccttgattg tcctgacctc attgttgatg gcgttgcttc tgaattccag ccacttcttc
     1921 accaagttct tcaggactgt cttcttcttg ccgacggcga tctcgttcgc cgttgtctcg
     1981 gtaatctgga tgatagtcct cgatccgtat aacgggctgg caaacagctt tctgaccatg
     2041 ttcggcttca aacctcagcc cttcttcgcc tctcccgatc aggcgctttg ggggctgata
     2101 cttcttaacg tctggagaag ttcgggatac tggatgatgt actttctggc tggaatgcag
     2161 aacattcccg agacactgta cgaagctgcc gagatcgacg gggcctcaac tttgagcaag
     2221 actttcaaga taacgctgcc tctgcttact aggaccattt catttgttct cgttgcaaat
     2281 acggccttca attttcttag tttcgcgccg gtctacataa ttaccaaggg cggccccatg
     2341 ggctcgacaa atctcctgat gtacgagtct tacaagagcg ccttcgtaaa tctcgacatg
     2401 ggaagggcct ctgcgatcac tacgatcttg ttgggaatca tccttgtttt cagtttcttc
     2461 gagctgagat ttaccagagc tcgattcgag tattgaggtg gaatcatgaa gaagcgaagc
     2521 ctttggaaaa cacttattct ctttgccgtt ctcggcatta taagtctttt ctttctgatc
     2581 ccactcatct gggtcgttgc atccgctttg aggcctgcca gtccgctcta cgagtacgcc
     2641 aatcctctga cctggaagag tttcattccg actcaaccga cgctggagaa tttcgtacat
     2701 atatttgtca acctcaactt tggcagggct ctcatgaaca gccttttcgt ttcggtgtcg
     2761 accatagtcc ttacggtcct ggtcgcctcc atggcaggct tcgcgcttgc aaagttcgaa
     2821 ttcagaggca aggctgcgct cttcacaata gtgctaatca cttttatggt tcccttcgag
     2881 tctattgtca taccgcttta tatactcata aaacagctac atatagacaa cacatactgg
     2941 gcgctcattc ttccgggcgt agccaacggc cttgcgatat tccttttcag gcagttcttc
     3001 tctgaagtac cttcagagat aatggaagcg gcaagaatag acggcgcgtc ctggttcaga
     3061 atctactgga ggatagtcct tccgctgagc gttccggcca tagtcacggt aatcgtaatg
     3121 gtcttcatgt tccagtggaa ctcgctgttc tggcctcttg tagcaaccca ttcaagcaga
     3181 ttcgaagttg tacaggtagc catcgccgca catagatcaa cggaaaatac aagttgggca
     3241 aatctcttca gctcggccat cgccggcagt ctgccgccgg taattctctt cctattcctc
     3301 cagaagtact tcgtaagggg gataagcgga acgggattga aaggatgatt tcatgaataa
     3361 ttcgaagaag gcgaaaaacc acttgcagat ttccaacgac tcccttcccg agatagctct
     3421 tctgcctggt gatccggcaa gagtttacga gatcggagag tgtctgagta atgtggagga
     3481 aataggaaac aacagagact acgtaaccgt taccggaacc tataaaggcc tgccacttac
     3541 tgtatgttca agcggaatcg gaggaccgtc gactgagatc gcggtggtcg agctaaacag
     3601 gctaggcgtt aagacaatta tccgtgttgg aacctccgga ggacttgccg acgatgtgaa
     3661 accgggagac ttgatcgtgc tttcgagttg cataagatac tcggggacag caaacctctt
     3721 cattccggag aacttcccgg ccgtagccga ttaccggcta cttacggcgt tgatctcagc
     3781 ctgtgaagag gccggtgtgg tctatcacgt gggaatcggt ctttcactcg attccttcta
     3841 tgcaacaaag cccgatctct taagagaaga cttcccatca tctatctacg gaaaacttga
     3901 agaatggatc gcggcaggtg ccttgcagct cgacatggaa gcggctactc tctatgtgct
     3961 ttcttctctt ctgaagatca acgccgccgc catatgcacc gcaggatcca acatctcacg
     4021 aggtgaaaga ccggagactc ctccttcgaa caaaaacgct ataagagcag cctgcgaagc
     4081 agcttataaa ttcaataact ggaaaaagat ctcagtgaag cgggggagga atttcaccct
     4141 tcctccgatt gcagagcggg ggtaatgaac aatgagtgag agactgaccg gaaagacggc
     4201 ccttatcttc ggagctggca gagttggaag ctccgctgcc aatgcatttn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gaaagatcat caacctggcc tcggtcggag
     4381 gcctctttcc ggaaaaggag cacgcaggcc actgcgcagc aaaagcgggg attattgcga
     4441 tttcaaaggt aatggcgctc gaactagccc cggccaatat tcaggtaaac gtcattgccc
     4501 ccggtccaac agagaccgtt ccatttactt ctcccttcta ttcccaacat cccgaagtac
     4561 tgaaaagaat agaggaaaag actccggccg ggaggattgg tcatcccgaa gaccatacag
     4621 gactaatggt attcctggct tcggatgaat cgaactggat aacgggccag gtgataatgt
     4681 ccgacggcgg acttggtctg gtgtgaatct tcaaaacaca gacgaataag tatgccccgt
     4741 atcgagccgg aacatattca ttggtgtcag gataatacta gaagtgttga agctcgaagg
     4801 gcaagctctt cggaagatcc tgacctttgt agccgtccga taccttaacc ctgccaaact
     4861 ctatcatctc tttgaaatta gtgctaccgc caacaaccga agacaaggtc gagatatcac
     4921 actcaagatc aaaagagtca ctcggcgaga agccaagttc tccattctca acacatagtt
     4981 cgaaaactcc gtcgttccag gatgcctgac tgtcgtcgat tttgattccg acccgtatgt
     5041 ccggagcgtc gattctcaga ccgtttagta gctggaggtc tacgattctg atcatagtcc
     5101 tgggtactat cttcatctcg gacggccttt cctttagata cggccagaga aggaaatcag
     5161 gcggaaggaa ggcgtggatc tgatcgattt gaagagaaag acctttcaaa aaacggaaca
     5221 ttgcatgcct tgtttcgaca tcgtccgcaa ggaagatcgt gactacaaac ttaagtccgc
     5281 tgtccttgtc aaacttatcg tagagaaaga cgtagcggag catccccgta gtccttcctt
     5341 ctcgcttgaa cttcacgaat ttcttggaaa catcgttatc ggttcgaaac tcgaaatcgc
     5401 ttttccacat ctcttcgtct ctcaagatca tgctgtagtg actgcttgcg tactcgttgt
     5461 agaactccct gatttcgccg tcggcatctt ccatctcctc aatcttcact tcgaccttct
     5521 gatcgggaag atctatagaa cctggggaga acttcgccct gagcatcgtg tcaaactcct
     5581 cccatccgag ctttctgtag aagctcctgc taaagggata gagcagagat actgcctgcc
     5641 ccttctctct catggtctcg agtgatttct ccagaaggaa tttgactgcg cccttacccc
     5701 tgtaaagggg acttgtacac acgttgccga ttccacccat cggaatgacg gaatctctga
     5761 gtcgcatctt caaagaaagt agaagatact cggcggcaag agtagaaccg tcataggccc
     5821 caaaggggtc ccttagatcg ttattgaaga tctcaccagc atgtttcgaa gcgaccctca
     5881 tccaattttc tgtaataccg aaagagaact tcataagatc aagataatcc ttctgattgc
     5941 ttattctcct gcattcaaac atcatttcac catccctgaa tgtggtgtaa aaatgatagc
     6001 atatcgccgt cgtcgtacga agaccgtggc tgggggtagc aggttagtgg catcaagatt
     6061 agaagtgatg ctgcctccgg caggaagtga ggctcgctgg cgcgaggaag tgatgcccgg
     6121 aaga
//
LOCUS       DAOQYI010000080         6030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_13412_length_6030_cov_51.388619, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000080 DAOQYI010000000
VERSION     DAOQYI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13412_length_6030_cov_51.388619"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKL93_10575"
     CDS             complement(<1..403)
                     /locus_tag="PKL93_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNQ71742.1"
                     /translation="MYDYVIWDFGGTLFNTYPAAAKVFQEVLEKYDVEASIDEILEKL
                     KESTSKAASYFVNKYKLKNGFLEDFYRIENLLQPERQPPFDNAKEICVKINRNGGSNF
                     LFSHRSNYSMTKLLNYYNMLDLFSEIVSADSG"
     gene            complement(472..1029)
                     /locus_tag="PKL93_10580"
     CDS             complement(472..1029)
                     /locus_tag="PKL93_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNQ71743.1"
                     /translation="MIIRGCGLSGIIVVEVTALQEEIVLDIMIEIPKGSRNKYEYDKK
                     FQRIRFDRTLFSAVHYPMDYGFILNTLAEDEDPLDAMVLLWEPTFPGCLIEAKPIGAF
                     KMWDEKGPDEKVLCVPIHDPVWNYIETLDDVPPHLLKEIEHFFSVYKDLEKKKTGIEG
                     WVRREETLEIIAASRVRYLEETHDS"
     gene            complement(1079..2545)
                     /locus_tag="PKL93_10585"
     CDS             complement(1079..2545)
                     /locus_tag="PKL93_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNQ71744.1"
                     /translation="MKKILVALLSVLFLYSFATANVATQVVSIETEINSAISRMNLSW
                     KADLEESFVAKLETANIGDVEAIFEKLNGYIDLPDEVRERFIEYLRLAYIEEMDEFSI
                     QGMTTTDDLMMSTFVLLEPRVVAESSFVRLEPIRDQFIHGSCWSFATVGSFESALAVQ
                     SFGMNGNVDNTFDYSERWGTYHNIDWDIYSFSPDSYVQDKNSLEGGNSYFAMYNLIRY
                     GMMEEEYAPYSEVYLDPREEVPLPPSAYNAPLLKSSRTVMIPPSTSAAELGYSYEDYL
                     NMIKTALSDYGSLAVSFSVPYSFNYYSKGIYSPVEGDYSTGGHAVTLVGWASATDLDD
                     IILAGKTNPDATPILDEEIESYTYVDPTQAASPIFTTTTFWIIKNSWAYDWGDGGYFV
                     IPAISEEQYESGQLGFWEIEKREMYLPIFDDLAHHEGDDLDVNSDGVVDTKDFEYLVE
                     KIGSTEADDITACDISFPKDGKINGEDAAAWVFLFNNR"
     gene            complement(2559..3899)
                     /locus_tag="PKL93_10590"
     CDS             complement(2559..3899)
                     /locus_tag="PKL93_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71745.1"
                     /translation="MKKTLLITLLLGFMLFVSGCFKINNPPVISLTGSVSEGQEIAPM
                     TLVTFEWEVTDFDDTEVTSQFKLYKDGAEVLSVVDTGEAVYALDEEGKYIAEVVATDS
                     MGAQSKETISFKATNLKRELFQSGAGLMYFDYSDYFQYIEDEAFGIRFYKTASEEIVP
                     AEEVTVRKRLNLLDTDDDGIYDTWEEIPNDRPDLQTYVYLPGYLIDYDTLWVPYGFEY
                     GEKSIRAYYAGVEGPYSGYVLFYKFAEVGFDLNTVYARWVEAHVFGNTFNDYGEAYKA
                     FSLRERYDSDEKPVISLSTPDSDIGPGDSFTVGVMTENVASFADLYDVRYIQLSLWNS
                     EGIVLESVEFGNFMAGLKDVGTFKSSDEAVTLYKAFLNGEDEAEAADNVFATLTFSVP
                     EDYEGEIIAVGLAYEGYWDTYDIYPDLPNPAFRNVDNGSVDGFIVDHEPIVFLE"
     gene            4213..>6030
                     /gene="leuS"
                     /locus_tag="PKL93_10595"
     CDS             4213..>6030
                     /gene="leuS"
                     /locus_tag="PKL93_10595"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNQ71746.1"
                     /translation="MYDFRAIEKKWQEYYAKEKPFNIDLEKAERPFYNLMMFPYPSAA
                     GLHIGNMFSFIGSDVYGRFMKLKGYDVFEPIGFDAFGIHSENYALKVGRHPAELTPKS
                     IEYFREEQLKKIGNIFDWNSEVITSSPEYYKWTQWIFIQLFKNGLAEKRSSNVNWCPS
                     CKTVLADEQVIDGRCERCNSEVEKREMSQWFFKITEYAEKLLSNLEKLDWTDTTKNLQ
                     RAWIGKSSGATISFEVSGMNEKIDVFTTRPDTIFGVTYIVVAPEYGLVSKLITPSTLQ
                     AFENFKEEVKNMDLATRTSISRPKNGIFTGSYAVHPLTGEELPIWIGDYVLAEYGTGA
                     VMAVPAHDERDYVFAKKYGLEIKQVIECDPSQLPYTEKGKMINSGKYSDMLSVDFIER
                     IDEVSESIKGSVNYHLHDWCISRQRYWGPPIPIVYCDKCGTVPVPEDQLPVTLPNTDD
                     YIPDGSGKSPLAKNEEFVNTICPVCGGPARRETDVSDNFLDSAWYYLRYLSPKNNEFA
                     FERELVEKWCPVDMYIGGNEHATLHLMYSRFLSMALHDMGFLPFEEPFTSFRGHGLII
                     KDGEKMSKSKGNIVNPNEYFESHGVDALRTYLMFMGTFLE"
BASE COUNT         1663 a         1417 c         1276 g         1674 t
ORIGIN      
        1 agccgctatc cgcagatacg atttcggaga agagatcgag catattgtag taattgagaa
       61 gtttggtcat ggagtagttg cttctatgac tgaaaaggaa attgctgccg ccgttcctgt
      121 tgatcttcac acaaatctcc tttgcgttat caaatggcgg ctgtctctcg ggttgcagaa
      181 gattctctat gcggtagaaa tcctcaagga agccattttt cagcttgtat ttattcacaa
      241 aatagcttgc agctttcgac gtcgactcct tgagcttttc aaggatttca tctattgagg
      301 cttcgacatc atacttctcg agaacttcct gaaatacctt tgctgcagct ggataagtat
      361 tgaaaagtgt acctccgaaa tcccaaatca cgtaatcata catagtactt cccctttctt
      421 ggatctaaat caataataaa cgcttttggg gatttcgtcc tgtcgagtat cttaagaatc
      481 atgagtttct tcaagatatc ttaccctcga tgcagcgatt atttccaacg tttcttctct
      541 tcttacccag ccctcgatac ccgttttctt cttttcgaga tccttgtata cggagaagaa
      601 atgctctatc tctttcagca gatgaggcgg aacatcatcg agagtctcaa tgtaattcca
      661 gaccggatca tgaatcggaa cacaaaggac cttttcatcg ggtcccttct cgtcccacat
      721 cttgaatgcg cctatgggct tcgcttctat tagacagcca ggaaaggttg gctcccacag
      781 caaaaccatc gcatcgagcg ggtcctcatc ctcggcaagc gtattgagga tgaagccata
      841 atccattgga taatggaccg cagaaaacag cgtcctgtcg aatcttattc tttgaaactt
      901 cttgtcgtat tcgtacttgt tcctgctccc tttcggaatc tctatcatta tgtccagtac
      961 tatttcttcc tgcaaagctg tcacctccac aacaattatg ccagaaagac cacagcctct
     1021 gattatcata aaagaagaac cccataaggg gttcttctaa accgatttca aaacagtctt
     1081 acctattgtt aaacaagaat acccaggcag cagcgtcttc gccgttgatc ttcccgtcct
     1141 tggggaagga aatgtcgcaa gcggttatgt cgtctgcttc cgtagaaccg atcttttcaa
     1201 ctagatattc gaaatccttc gtatcaacaa cgccatcgct attgacgtca agatcgtcac
     1261 cttcgtgatg agcgagatca tcgaatattg gcaggtacat ctctcttttt tcaatttccc
     1321 agaagcccag ctgcccactt tcatattgtt cttcactgat agccggaatc acgaagtaac
     1381 caccatctcc ccagtcatag gcccaggagt tctttattat ccagaacgtg gtggttgtga
     1441 aaatcggtga ggccgcctgt gttggatcga catatgtgta gctttcaatc tcttcgtcca
     1501 gaataggagt tgcatctgga tttgtcttgc cagctagaat aatatcgtcg agatctgtgg
     1561 cacttgccca tccgactaga gtaactgcat gtccgcccgt actataatct ccttctactg
     1621 gagaatatat accctttgaa tagtagttga aactatacgg aactgagaaa gagacggcaa
     1681 gagatccata atctgaaagc gctgtcttga tcatgttcaa atagtcttca tagctgtacc
     1741 ctagctcagc tgccgatgtg gatggaggga tcataacggt tcgggacgac ttcaagagcg
     1801 gagcattgta tgccgaggga ggcagaggga cttcctccct ggggtccaaa tagacttcag
     1861 aatacggggc atactcttct tccatcattc cgtacctaat taggttgtac attgcaaaat
     1921 aactgttacc gccctcaagg ctgttcttat cctgaacata actgtctggt gaaaaactgt
     1981 agatatccca gtctatgttg tgataagttc cccatcgttc cgaataatca aaggtgttat
     2041 caacgttgcc gttcattccg aaagactgca ccgccaaagc gctctcaaaa gagcccacag
     2101 tggcaaaaga ccagcagctt ccatgaatga actgatctct tataggttcg agtcttacaa
     2161 aactgctctc ggctactacg cgaggttcca gaagcacgaa agtcgacatc atgagatcat
     2221 cagtagttgt cattccctgt atagagaatt catccatctc ttctatgtat gccaacctca
     2281 aatactcgat gaatctctcg cgcacttcat ctggaaggtc tatgtagcca ttgagtttct
     2341 cgaatatcgc ttctacgtca ccaatgttcg ctgtttccag tttagcaacg aagctctctt
     2401 ctagatctgc tttccagctg agattcattc ttgatatcgc tgagttgatt tccgtctcta
     2461 tggatactac ctgagttgca acgttagcag ttgcaaacga gtataagaac aaaaccgaaa
     2521 gtaatgctac caatattttc ttcatggtca tccccccttt actccaagaa gacaatcggt
     2581 tcgtgatcaa ctatgaaacc gtcaaccgaa ccattgtcta catttctgaa agcgggattt
     2641 ggcaaatcgg ggtaaatgtc atatgtatcc cagtaacctt cgtacgccag tccaacggca
     2701 atgatctcgc cttcataatc ctctggaact gaaaaagtga gagttgcgaa gacattatcg
     2761 gcagcttcag cttcatcttc tccgttcagg aaggctttgt aaagggtaac tgcttcatca
     2821 ctgctcttga atgttccgac gtccttcaaa ccagccatga aattcccgaa ctcaacgctt
     2881 tcaagaacaa tgccttctga gttccataga gaaagttgaa tataccttac atcgtaaaga
     2941 tccgcaaaac ttgcgacatt ctctgtcata acaccgacag taaatgaatc gccgggtcct
     3001 atatcgctat caggagtaga aaggctgatc acaggttttt catcggagtc gtatctttct
     3061 ctcagagaga aggctttgta tgcttctcca tagtcattaa atgtgtttcc aaatacatgg
     3121 gcctcaaccc atctcgcgta aactgtgtta agatcaaatc ccacttcagc aaacttgtag
     3181 aacaagacat atccactgta aggaccttca acaccggcat agtatgccct tatcgatttc
     3241 tcgccatact cgaatccata aggcacccat agcgtatcgt aatctatcaa ataaccaggt
     3301 agatacacat atgtctgaag atcgggcctg tcattcggta tttcttccca ggtgtcatag
     3361 atgccgtcat catcggtgtc gagcaggttt agtcgcttcc ttaccgttac ttcttcggca
     3421 ggtacaattt cctctgaggc agtcttgtaa aacctgatgc cgaaagcctc atcttcgata
     3481 tactggaaat agtcgctgta gtcaaagtac attagtccag ctccagactg gaagagctct
     3541 ctcttaagat tcgtggcttt gaaagaaatc gtctctttcg attgagcacc catcgaatcg
     3601 gttgctacaa cctctgcgat gtactttcct tcttcgtcta gagcgtacac cgcttcgccg
     3661 gtgtctacca cagacagaac ttcagcccca tccttgtaga gcttgaactg agaagttact
     3721 tccgtgtcat caaagtcagt tacttcccac tcgaaagtga caagcgtcat cggagcaatt
     3781 tcctgccctt ccgaaacgga gcctgtcaac gatattaccg gtggattgtt gatcttaaag
     3841 cagccggaga caaatagcat gaatcccagc agaagagtaa tcaacaacgt tttcttcatt
     3901 ttcttacccc cttttcaaag atttcgagtt caaacaatta tacattcaca ggcgccaatt
     3961 ctgaaaaccc catttgtgaa caattctgct ttcatggctt gaaaacatga tttctgcttt
     4021 gtctagatcc tacctgacac cgtcggaaaa tctctgaaaa acttcgataa aggactcggc
     4081 aacatgcttt tcaatgaaga aatttacttc actttcaacc gggttattca tcccaaattc
     4141 agtaacactc ccatgttata atccatctaa gttgcttttc taaagcttta cattgattcg
     4201 gggaggtagc acatgtacga tttcagggcg atcgaaaaga aatggcagga atattacgct
     4261 aaggagaaac cattcaatat agaccttgag aaggccgaac gacccttcta caacctcatg
     4321 atgtttccct atccctccgc agccggactt cacataggaa atatgttttc cttcataggt
     4381 tcggatgtat atggaaggtt catgaaactg aagggctatg atgtatttga accgatcggc
     4441 ttcgatgcct ttggaattca tagcgagaac tacgctttga aagttggtag acatcccgca
     4501 gaactcaccc cgaagagcat agagtatttc agagaagagc agctgaaaaa gatcgggaat
     4561 atcttcgatt ggaacagcga agtgataacc tcttctccag agtactacaa gtggacgcag
     4621 tggatattca tacagttatt caagaatgga ctcgcagaaa agcgttcttc aaatgtgaac
     4681 tggtgtccat cgtgcaaaac ggtgcttgct gatgaacagg ttatcgatgg tcgttgtgaa
     4741 aggtgcaaca gcgaagttga gaagcgtgag atgagtcagt ggttcttcaa aatcacagag
     4801 tacgcagaga agctgctgtc taacctggag aagcttgact ggaccgatac gaccaaaaat
     4861 cttcagcggg cctggatagg caaatcctcc ggagctacga tctcttttga agtatccgga
     4921 atgaatgaga agatagatgt ttttacaacc cgtccagata cgatatttgg agtaacttac
     4981 atagttgttg ctcctgaata tggtctggtc agtaagctaa tcacgccctc aacactgcag
     5041 gcctttgaaa acttcaaaga agaagtcaag aacatggatc ttgccacgag aacatctatt
     5101 tcgcgcccca agaatggaat attcaccgga tcttacgccg ttcaccctct tacaggtgag
     5161 gagcttccaa tatggatagg agattacgtt ctggcagaat acggaacggg cgctgttatg
     5221 gccgtcccgg cacacgacga gagagattat gtctttgcga agaagtatgg actggagata
     5281 aaacaggtta tcgaatgtga tccctcacag ttaccctata ctgagaaggg aaagatgatc
     5341 aacagcggaa agtattcgga catgctcagc gttgacttca tcgagaggat agatgaagtg
     5401 agcgagagta taaagggttc ggtgaattac caccttcatg attggtgcat ctcgaggcaa
     5461 cgctattggg gtccgccaat tccgattgtg tactgtgata agtgtggaac ggttccggta
     5521 cctgaagatc agttgccggt aactctacca aataccgacg attacatacc ggacggaagc
     5581 gggaagtctc ctttggcaaa gaatgaagag tttgtcaaca cgatttgccc tgtttgcggt
     5641 ggaccggcta gacgagagac cgatgtatcc gacaatttcc ttgattcggc ttggtactat
     5701 ctgagatacc tctctccgaa aaataacgaa ttcgccttcg aaagagagct cgtcgaaaaa
     5761 tggtgccctg tagatatgta tattggcggg aatgaacatg ctaccctgca cctcatgtac
     5821 tcgagatttc tttcaatggc acttcacgat atgggttttc ttccttttga agagccgttt
     5881 acctcattca gaggtcacgg actcataatt aaggacggcg agaagatgtc gaagtccaag
     5941 ggcaacattg tcaatccaaa cgaatatttt gagagccatg gagtagatgc cctcaggaca
     6001 tatctgatgt tcatgggtac gtttcttgaa
//
LOCUS       DAOQYI010000081         5965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_13590_length_5965_cov_62.505584, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000081 DAOQYI010000000
VERSION     DAOQYI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13590_length_5965_cov_62.505584"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..667)
                     /locus_tag="PKL93_10600"
     CDS             complement(5..667)
                     /locus_tag="PKL93_10600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ71747.1"
                     /translation="MMVRGIIFDLFGTIVSNARLFRPVCSKMAQESSAEVVEIERDFV
                     NLYGHYFKDCHKMPFQPERYYYYLLISDLIDKYDLPGDMEAYCNFMYDSFSKFRAYPD
                     ARILRAIVNKYSVAILTNADNSFAEKVVKRNKIPHHFLLTSESARSYKPCEVIFEKLL
                     SMMEIEKRHVIFVGDSIQVDMLGASAAGIKGILIDRSRSYLDFAPRIESLEELPSLLE
                     SL"
     gene            794..1471
                     /locus_tag="PKL93_10605"
     CDS             794..1471
                     /locus_tag="PKL93_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71748.1"
                     /translation="MSREMRIMWLHNRLLKNDFAAMKDYTQKFGISVRQAHRDFKYLR
                     ANLGAPMKYSRKRGEYFYSEPYHLPSLFEDSMKFQLRTEYRISSVFLNAIASKKAVKI
                     FQRGGKEFIFYPACFDERRELFCGLQEDGNVRFVRSDEIDKVIFSNKRYLEEPMLWNR
                     IFPREAEFHEVDLDFGGDRHKYHFFEIGDLVMFLASENSFKVIGPQEIIDELRKVAEN
                     LLKTIAD"
     gene            2362..2742
                     /locus_tag="PKL93_10610"
     CDS             2362..2742
                     /locus_tag="PKL93_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71749.1"
                     /translation="MVYAPKAGHGAEVFRVSQAISSMIHHLNTGKPLSSMSYVEEQSE
                     GTLKVRTLMEKENANLKELRLFRSSSPDGDFRKTRFESIHVKECNSKELTLKAHPVLF
                     RRRFRLWWKGALDIDIRESYGQMT"
     gene            2805..3155
                     /locus_tag="PKL93_10615"
     CDS             2805..3155
                     /locus_tag="PKL93_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ71750.1"
                     /translation="MSELCPSREIHVDVDKYRLIAKEVSGLSDLFKVIADETRTKIVF
                     LLSETELCTCDLAEILRLSLPTISHHLKQLKSYRLVKSRREGKSVFYSLEDFHVVELI
                     KLAREHFQELSEGD"
     gene            3157..5205
                     /locus_tag="PKL93_10620"
     CDS             3157..5205
                     /locus_tag="PKL93_10620"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNQ71751.1"
                     /translation="MKKYRIEGLDCPKCAAKLEDQLKRSGVDARIDFSSSTLIIDGQT
                     FEEVEEVIHKHSPEITLLESPDVEKDLPVKELVKLLFSGVLFFIALVFSPFIHGNNEL
                     LEYGLFVTAYLVAGGNTVLKAFRNLFKGDLFDESFLMTVATMGAFAIHELPEAVAVMI
                     FYSFGELLEAIALRKSRRSVKELIDLRAEEVHQIIDGKLRTVPASQIQPGDRFVVKPG
                     ERIPIDGLVNSGSTWIDKSLITGESSPVPVKEGDIVLAGTINKSGVIEVTASTNLQKS
                     YTSRMLELVEEGTSRKSNRERFITRFSRLYTPIVVSISTAIAVFPPLLFGGEFSDWVH
                     KALILLVISCPCALIISVPLSYFVGIGKASREGILFKGSNYLESLGKIDSIFIDKTGT
                     LTKGRLEISQIETSGRYDKRELLEYAALMESYSTHPVASSFRSFIPEIREELKISHYS
                     EIAGKGITAEINDKKMGIGNSSLLKDLGVDYKERGESGKIFLSIDGRVEGVFTLSDVL
                     KDDSADALIKLRELGIHEIVMLTGDSHDIAGSISAKLDLDGLISEATPEDKMRAVEDA
                     IKSGKRTAFVGDGINDAPVIARADVGIAMGFSGADAAIETADVVLSGESLSKVARAIE
                     ISRSTNRNAIQNITAALGVKMAFIALGAFGLMNMWGAVFADVGVTLLAVLNSLRMLNK
                     "
     gene            complement(5317..>5965)
                     /locus_tag="PKL93_10625"
     CDS             complement(5317..>5965)
                     /locus_tag="PKL93_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ71752.1"
                     /translation="KMRHSTRKFLPYGLSSEERERLVDFFEVIPTLHSLRLKWALKDL
                     NKGSSAIYAPCTNKPNCLVEYGFQGEIIVLEVTKMGFGSCWNAGIREAGSPAGIIIGK
                     EDQGKVSINDILTGMGRRKELRALIDGPVPEDERLLQILESCRLAPSSMNRQPWRFSV
                     LEGDLYIWTKANMIGGGHWLDLGIVLSHAYITALEFFKNVSIEKAAGDRYRVIMK"
BASE COUNT         1717 a         1264 c         1342 g         1642 t
ORIGIN      
        1 gctcttacaa ggactctagc aagctaggca gttcttccaa gctttcgatt cttggtgcga
       61 agtctaagta agatcttgac cgatcgataa gaattccctt aattccggct gcagaagcgc
      121 caagcatgtc tacttgaatg ctgtcaccaa caaaaatgac gtgcctcttc tcgatttcca
      181 tcatagacag tagcttctca aagataactt cgcatggttt gtacgatctt gccgattccg
      241 aagtcagcag aaaatgatgt ggaattttat ttctttttac aaccttctca gcaaaggaat
      301 tatcggcatt agttaggatt gcaacggaat atttgtttac gatcgccctt aggatccttg
      361 catccggata ggcgcggaac ttagagaagg aatcgtacat gaagttacag taggcttcca
      421 tgtctcctgg caaatcgtac ttgtcaatca aatcgctgat cagaagatag taatagtacc
      481 tctccggttg aaagggcatt ttatgacagt ccttgaagta gtgaccgtag agattcacaa
      541 agtctctctc tatttccacc acttcagcgg acgactcttg tgccatcttg gagcatacag
      601 gtctgaaaag cctcgcatta ctgacaatgg ttccaaacaa gtcaaatatg attcctctaa
      661 ccatcatgtc tccatttcat cttctagatt tacttttcga atttttgtat aatactattg
      721 caattctagc accttagaat tctgttctgc gatgttcaga tgtctctcgt ctgggccact
      781 ggaggtggca gatgtgtcta gggaaatgag gattatgtgg ctgcacaatc gcttgctaaa
      841 gaatgatttt gcggccatga aagactacac tcagaagttt ggcatctcag tccgacaggc
      901 ccatagagat ttcaagtatc taagagcaaa tcttggagcg cccatgaaat actctaggaa
      961 aagaggcgag tacttctatt ccgaacccta tcatttgcct tctctcttcg aagacagcat
     1021 gaaatttcaa ctgagaacag aatatcgaat aagctcagtc tttctgaacg cgatagctag
     1081 taagaaagct gtgaagattt ttcaaagagg aggcaaagag ttcattttct atcccgcctg
     1141 ttttgatgaa agacgtgagt tattttgtgg tcttcaagag gatggtaacg tccgatttgt
     1201 caggtcagac gagattgata aggtcatctt ttcgaacaag agatatttag aagaaccaat
     1261 gctctggaac cgaatattcc cgcgagaagc tgaattccac gaagtagacc tggattttgg
     1321 tggcgatcgc cataaatacc atttcttcga gataggagat ttggtgatgt tcctggcttc
     1381 tgagaactcc ttcaaggtta ttgggccgca ggagatcatt gacgaactca gaaaagttgc
     1441 cgaaaatctt ctgaagacaa tagctgactg agatgagagc aattccagtt ttcttgctct
     1501 tgattgttgc ggcctctctt tcatcggtta tggtggatag ttttctgaac tccactcaat
     1561 tccatcaact atggataatg caaatgtcaa gattgtggag tcgccttcag aggggcttat
     1621 cgaaatagat acgttaaact tcgactcggt tgtattgcag aggattaagt ggctaaacga
     1681 tccgatgatc ataaaaccac caaggataga gtcaaaagtt gcagtgatgt tcataactgg
     1741 tgaccacgga tactcgtagg aagagctggc attattcgga ctgctggctt tgcaaataga
     1801 gcttacgttg ccgtcctatt caacatccca aatcaaccac tttttggcgg tcccaaagag
     1861 gactggttac ttagctattc tcttgtaaga tgtcttaaga gttgtgatta cacatgaccc
     1921 gccttattgc ccatggttca agccactatt gcttcaatga acatcatgaa tgacttcata
     1981 gggaacaagg gggacggagt tgaaggcttc attttatccg ggagtccaag cgaggttgaa
     2041 ccacatggct aactgccccg atgcacaaaa gagtcaaagg aatcgttccc acagcctttg
     2101 acaatctcaa tattgctgta cagatggaac acaagcttga cttctgggga ggcttttgtc
     2161 actccattga ggaatatgta cagagatgaa tccttgatga tctaaataac ccggcataac
     2221 tagatttgcc agagtacacg gatctttcac atatcgatct caactcgtaa ttccaaagca
     2281 aattgctgtc ggaacgaatg atccatatgg cccatagact cagctaacta atacatagaa
     2341 gaactgcccg gctgttcatc aatggtctat gctccaaagg caggacatgg ggccgaagtt
     2401 ttcagagttt ctcaggcgat aagttcaatg attcatcacc tgaacacagg taagcctctt
     2461 tcttcaatga gttatgtaga agagcaatca gaaggaacgt tgaaggttag aaccttgatg
     2521 gaaaaagaaa atgcgaatct aaaggaactg aggctattca gatcgtcttc cccggatgga
     2581 gattttcgaa agacaagatt cgaatcgatc catgtcaaag aatgcaattc aaaagaactg
     2641 actctcaaag cccaccccgt tttatttcga aggcgttttc gactttggtg gaaaggagct
     2701 cttgatatcg acatccgcga aagttatggg cagatgactt gacttgagaa gtgaaagcaa
     2761 ttataatcaa ttagatgacc atctaattga tttggagaca tcctatgagt gaactttgcc
     2821 cgagtagaga aattcacgtc gacgtagata aatatagact gatagctaag gaagtttcgg
     2881 gtctctcgga tcttttcaag gttatcgccg acgagacgcg aacgaaaata gtatttcttc
     2941 tgtctgaaac ggaactgtgt acatgtgatc ttgcagagat tctccggcta tcactgccaa
     3001 cgatttccca tcacctaaag caactgaagt cttacagact cgtgaaaagc cgtagggaag
     3061 gtaagtctgt tttctactcc ttagaagact ttcacgttgt tgagctcata aaacttgcca
     3121 gagaacattt ccaggaactg tcggagggcg attagaatga agaaatacag aatagaagga
     3181 cttgattgtc ctaaatgtgc agccaaactg gaggaccagc taaagagatc gggagttgat
     3241 gcaagaatag atttctcttc ttcaacacta ataatcgatg gtcaaacttt cgaagaagta
     3301 gaagaagtca ttcacaaaca ttcaccggag ataacgctgt tggaatctcc ggacgttgag
     3361 aaggatttac ccgtcaaaga acttgtgaaa ctgctcttct caggagtcct gtttttcatt
     3421 gctttggtct tttctccctt tattcatggg aataatgagt tgcttgaata cggcttattt
     3481 gtaactgcgt atcttgtcgc tgggggaaac accgttttaa aggctttcag aaatctcttc
     3541 aagggggact tgttcgacga gagttttctt atgaccgtcg ctactatggg tgcatttgca
     3601 atccacgaac tgcctgaagc tgttgccgtg atgatctttt acagtttcgg agagcttctg
     3661 gaagcaatcg ccctgagaaa atcaagaaga tcggtgaagg aactgataga tttaagggct
     3721 gaagaggtcc atcagatcat tgacggaaaa cttcggactg taccggcaag tcagatccag
     3781 ccgggtgaca gatttgttgt caaaccagga gagagaattc ccatagacgg tcttgtcaac
     3841 tctggaagca cctggataga taaatcactt atcacaggag aaagtagtcc cgtgcctgta
     3901 aaagaaggcg atatagttct tgccggaacc ataaacaagt caggtgtaat agaagtcact
     3961 gcatccacaa atcttcagaa atcttacact agcagaatgc tggagttagt tgaagaggga
     4021 acttctagaa aatctaatag ggagcggttc attaccaggt tctcgaggct gtacaccccc
     4081 attgtggtct ccatctctac cgcaatagca gtatttcctc ctctgctgtt cggaggggaa
     4141 ttctcagatt gggttcataa ggccttgatc cttcttgtca tttcctgccc ctgcgcatta
     4201 atcatctctg ttcccttaag ctatttcgtc ggtattggaa aggcctcaag agagggaata
     4261 ttattcaagg ggagtaatta tctggaatct cttggcaaga ttgattcgat tttcatagac
     4321 aaaacgggaa cattgaccaa gggaaggcta gagatctctc aaatagaaac ttctggccga
     4381 tatgacaaga gagaattatt ggaatacgcg gcccttatgg aaagctactc cacccatcca
     4441 gtagcaagtt cgtttagaag ttttatccca gagattcgcg aagagctgaa aatctctcat
     4501 tattcagaaa tcgctgggaa aggtatcaca gcagaaatca acgacaagaa aatgggcatt
     4561 ggcaactcct ctctgttaaa agatctcgga gttgattata aagagcgagg cgaatcagga
     4621 aagatatttc tttccatcga tggcagagta gagggagtat ttactctgtc agacgttttg
     4681 aaggacgatt ctgccgatgc cctaatcaag cttagagaac tcggaattca tgagatagtt
     4741 atgctcacgg gtgatagtca tgatattgct ggttctatat cggccaaact ggaccttgac
     4801 ggtctgattt ccgaagcaac tcctgaagat aaaatgagag cagtagaaga cgctatcaaa
     4861 agcgggaagc gtactgcatt cgtgggtgat ggtattaatg atgcaccggt aattgcaagg
     4921 gccgatgttg gaatcgctat gggcttctct ggagccgacg cagctataga gacggccgat
     4981 gttgttcttt caggagagtc tctatctaaa gtcgcaagag ctatagagat atcgagatct
     5041 acgaacagaa atgccatcca gaatattaca gcagccctcg gagtgaaaat ggcatttatc
     5101 gctctaggcg cgtttggctt aatgaacatg tggggggcag tatttgccga tgttggggta
     5161 actttgcttg cagttctgaa ctcactcaga atgttgaaca aatgaggcac tgtgagtctt
     5221 caaagctctt cccgcataaa cctctcgggg atgctaccat atcaatgtgc acatcaagtc
     5281 actccaaatg tagaggatct ggtctggcat tagggattac ttcattatca ctctgtaacg
     5341 atccccggca gccttttcta tagagacatt cttaaagaac tctaaagcag ttatgtatgc
     5401 atgtgaaaga acaattccaa gatctaacca gtgacctcct ccaatcatgt ttgcctttgt
     5461 ccagatgtat agatcgcctt caagaacact gaatctccac ggctgcctgt tcatggacga
     5521 aggggctagt ctacacgatt caagtatctg aagcagtctc tcgtcttcgg gaactggtcc
     5581 atcaatcaga gctcgaagtt cctttctcct acccataccc gtaagaatgt cgttgatact
     5641 aactttcccc tggtcttcct taccgataat tatacctgcc ggcgatcccg cctctctgat
     5701 accggcattc cagcaactac caaagcccat tttcgtcact tccaggacaa taatctctcc
     5761 ctgaaagcca tactccacga gacagtttgg cttattggta caaggtgcat agatcgcgga
     5821 tgatccttta tttagatcct tcaaggccca tttcagacgc aaggaatgga gagtgggaat
     5881 aacttcaaaa aaatcgacaa gcctttcccg ctcctcagaa gagagtccat atggaagaaa
     5941 ctttctagtc gaatgtctca tcttt
//
LOCUS       DAOQYI010000082         5892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_13818_length_5892_cov_92.882817, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000082 DAOQYI010000000
VERSION     DAOQYI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13818_length_5892_cov_92.882817"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(820..1917)
                     /locus_tag="PKL93_10630"
     CDS             complement(820..1917)
                     /locus_tag="PKL93_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71753.1"
                     /translation="MSKTSVSVRIENVTKVFKDVKGKADVIAVNNANFEIEPGELVTL
                     LGPSGCGKTTTLRMIGGFELPTKGKIYLGNEDITYLPPNKRDTATVFQSYGLFPHMNV
                     FDNVAYGLKLRKLPSKQITEKVMSTLDLVGLKNLAQRAPSKLSGGQQQRVALARSLIV
                     EPSVLLLDEPLSNLDALLREQMRVEIKRIQKALGITTIYVTHDRVEAMSLSDRIIVMK
                     NGFIRQIGSPNEIYEDPNSKFVSGFVGKVAFLPVEVLEMNGKCKVRFEDKTVEVGKYS
                     TELSPGDSAVIMARPESLKLLDPGEGMIEGQVTTNIYLGGSLESFVTTGHGEILVQID
                     NPAEKKVYAEGEKVSIGIIPNLAKVLPDNDE"
     gene            complement(1941..4154)
                     /locus_tag="PKL93_10635"
     CDS             complement(1941..4154)
                     /locus_tag="PKL93_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ71754.1"
                     /translation="MIKLYSWRKLLSFGFISIAVFIALFWIFDTLKNSFFDVVRENQR
                     QLVTVIAESAPRDVETVKDWIVAVNEEFTEAEILYINGVPGWDELQVYAPNEQLKVFY
                     ESISSSEDFEKGLESVYYLENYYSSLDYTFEERNVSLVLAPLIDETRYNMTGFLVFLM
                     SAEASENYANLLNIFMFGAFAIFVIVFFISKFFRDPVMGYIILGLFLMVAIFVAYPLF
                     EAIRLTFLADGKFSMQTWVTILSSKQYLSALWGSVKLGILTASFSTIIGFIFAFVINR
                     TATRGKKFLSTMGLLPVISPPFSLSLSLILLFGSNGLITKQILGLKDFTIYGLGGLTA
                     VQTIGMFPIAFMTLSGVLQAIDSTLEEASLDLNGSRWKTFSKVTFPLAIPGVLSSWLL
                     VFTNSLADFANPLLLAGSYKVLSVEAYIEVTGRNRLGHGAALSILLLMPTLTAFLIQR
                     FWVSRKSYVTVTGKPSSRLSDLVSKPVKFGLITFIVIFIVFILGLYGTIVAGCFVANW
                     GIDYSFTLKNITEALQRGRDAIVDTFTLSAVATPIAGIIAMMAALVIVRKKFAGKRVL
                     EGLILAPFAIPGTLIGISYVLAFNKPPLILVGTGAIIVINYIIRELPVGVEGGVATLR
                     QIDPAIEEAAQDLGADSTTVFRTIVLPLIRPAFISGMSYTFVRSMTAVSAIIFLISAQ
                     WYHMTVLIYNFSESIRFGLASVLSTVLIIIVFGAFGLMRVLVRKNEYMEKNVTLN"
     gene            complement(4230..5231)
                     /locus_tag="PKL93_10640"
     CDS             complement(4230..5231)
                     /locus_tag="PKL93_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71755.1"
                     /translation="MRRFLILFTVICAAFSVTLLAADSFTVYTTLEELAAKQLFDKYE
                     EVTGIRVDWVRLSGGEAEARMEAEKLNPQASIWVGGVGLNHISAKLKGLTTPYFSRAA
                     ANTPDQYKDPEGYWIGLYLGPLAFATHNGRAAELGLEPPKGWFDIIDSKYKGLIRVAN
                     PNTSGTAYNLITCMISLFGRNEDLAFHYLKKLDENIEMYTRSGSAGGKSVAIGEIPFA
                     IGYAHDMVKLKAEGADITITIPEEGTGFEIASMSLIKGGPDPVNAKKLYDWILTEEAQ
                     AIIASWYVIPVSKIAPKHPLSFSMDEIKTVHQDYVWDAENKERLLDRWTKEIGGAAE"
     gene            complement(5335..5460)
                     /locus_tag="PKL93_10645"
     CDS             complement(5335..5460)
                     /locus_tag="PKL93_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71756.1"
                     /translation="MVDLRRLPAEGSIVSYVPSSFVGRPFFSKKVEKCYGSFKSS"
BASE COUNT         1536 a         1478 c         1385 g         1493 t
ORIGIN      
        1 ctgttgacat agtagaagcc catcgtccaa gagcaaggac ccgtccttcg aagagaagtt
       61 gcaagttgaa agcgctaaag tgaagaaaca tcaaatgccg tcattccgac aaggctcctg
      121 gtcggaatct taatgctctt gcttcattgc ctctaactaa gaacgaagaa cttggacgcg
      181 gagcgtcgga acgaggaaca agctttttct tcggcgaacg cagagccatt cagcggtgat
      241 gcggtttcac tctggtcttt caaacgtgca acccgctaac ctcggccatg gttttcaaaa
      301 aaccagatcc cggatcaagt ccgggatgac agacttaggt tattcggaaa acatcacatc
      361 acatgacagc gtcctgtaac tcttaaaaac acaacaaccc gtcattcaga caaagctcct
      421 ggtcggaatc tggatgctct tgcttcatcg ctctaaccaa aaacgaagaa cttggacgcg
      481 gagcgtcgga acgaggaaca agctttttct tcggcgaacg cagagccatt cagcggtgat
      541 gcggtttcac tctggtcttt caaacgtgca acccgctaac ctcggccacg gttttcaaaa
      601 acccagatcc cgaaacaagt tcagcatgaa gcagtgttag attctctgaa caatcttgat
      661 cttcctacat ctatcctcaa acctctgacc ccggcctttt tttcaactca caacttgtat
      721 cttgcaactg atcctttgct cttaccaacc cgcaaccccc taccccgaac ctcgcttgca
      781 acgcctacca atcaagggct tccacgattc cagcatctgt tattcatcgt tgtctggcag
      841 aactttggcc aggttaggga tgattccgat tgagactttt tctccttcgg catagacctt
      901 tttttcggcg gggttgtcta tctgcaccag tatctctccg tggccggttg taacgaagga
      961 ctcgaggctt ccgcccagat agatattagt tgtcacctgt ccttctatca tgccttctcc
     1021 gggatcgagc agcttgaggg attcaggcct cgccattatt acggccgaat ctcctggaga
     1081 caactcggta gagtactttc caacctctac ggttttgtct tcgaacctga ccttgcattt
     1141 gccgttcatt tcgaggactt cgactggaag gaaggcgact tttccaacga atcctgaaac
     1201 gaatttggag ttcgggtctt cgtaaatctc gttcggcgag ccgatctgtc taataaaccc
     1261 gttcttcatg acgattatcc tgtcagagag actcattgcc tctaccctgt cgtgggtcac
     1321 gtatatcgtt gtgattccca gtgccttctg gatgcgcttt atttcgactc gcatctgttc
     1381 tctcagcagg gcgtccagat ttgaaagagg ctcgtctaga agcaatacgg aaggttccac
     1441 gattaagctt ctcgcaagtg cgactcgttg ctgctggccg ccggacagct ttgagggagc
     1501 tctctgggca agattcttca agccgacgag atctagtgtg ctcattacct tttccgttat
     1561 ttgtttcgag gggagttttc ttagcttgag gccgtacgcc acattgtcga aaacgttcat
     1621 gtgagggaaa agcccgtaac tctgaaagac agttgctgta tcccttttat tgggcggcag
     1681 ataggttatg tcttcattgc cgagatagat ctttcccttc gtgggaagtt cgaatccccc
     1741 gatcattctc aaggtagtgg tctttccgca ccccgatggc ccgagaaggg ttaccaactc
     1801 tcccggttct atttcgaaat tggcattgtt gacggcaata acatctgcct tcccttttac
     1861 gtctttgaag actttggtta cgttttctat tcgaactgag acggaagtct ttgacatcaa
     1921 accacctcac aaacctagtg tcagttaaga gttacgttct tttccatgta ttcgttcttt
     1981 ctaacaagaa ccctcatcag cccgaatgcc ccaaatacga tgattattag aaccgtagaa
     2041 agaacgctgg caaggccgaa cctgatagat tcggagaagt tgtatatcag gactgtcatg
     2101 tgataccact gtgcagatat caagaatatg attgcgctaa cggcagtcat tgaccggaca
     2161 aaggtgtatg acatcccgga aatgaaggct ggacgaatca gaggcaagac gatagtcctg
     2221 aagaccgtgg tcgaatcagc gccgagatcc tgcgcggcct cttctattgc cggatctatc
     2281 tgccgtagag ttgctacgcc cccctcaacc ccaactggga gctctctgat tatgtagttg
     2341 atcacgatta tcgctccagt accgaccagg attaagggcg gtttgttgaa ggccaggaca
     2401 tacgaaatac ctataagcgt ccccggtata gcgaacgggg caagaataag cccttcaagc
     2461 accctctttc ctgcaaactt ttttctcaca atgaccagag ccgccatcat cgcgataatc
     2521 cctgcaattg gtgtcgctac cgctgacagt gtgaaagtgt ctactattgc atcccttccc
     2581 ctctgcaacg cttcggtaat gttcttaagg gtaaaggagt agtctatacc ccagttggcc
     2641 acgaaacagc cggccacaat cgttccgtaa agtccgagga taaagacgat gaagataaca
     2701 ataaacgtta taagaccgaa tttcacaggc tttgaaacca gatcgctcag tcttgaagag
     2761 ggttttccgg ttacagttac gtaggacttt ctactcaccc agaatctctg aattaagaag
     2821 gccgtcagcg tgggcatgag caggagtatt gaaagagctg ctccgtggcc aagtctgttt
     2881 cttcccgtaa cttcaatata tgcctcgact gaaagaacct tgtacgagcc tgcgagaagc
     2941 aggggatttg cgaagtcggc cagcgagttt gtaaaaacga gcagccagga actcagaacc
     3001 ccaggaatcg cgagagggaa cgtaaccttt gagaatgttt tccaccggct tccgttgagg
     3061 tcgagagagg cctcctccag agttgagtct atggcctgca ggacgcccga aagagtcatg
     3121 aatgctatag ggaacatgcc gatagtctgt acggccgtta ggcctccaag gccgtaaatg
     3181 gtgaagtcct tcagcccgag tatctgtttg gtaattaggc cgttactacc gaagagaaga
     3241 ataagcgata gagacagtga aaatggagga gagattacag gcagaaggcc catagtgctg
     3301 agaaacttct ttcccctcgt ggcagtcctg tttatcacaa aagcaaatat gaatccgatg
     3361 atcgtcgaga atgaggcagt aaggataccg agcttcacgc ttccccatag cgcactgagg
     3421 tattgctttg acgaaagaat cgtcacccag gtctgcatgg agaattttcc gtctgccaga
     3481 aaggtcaacc tgatggcttc aaagagcgga taagcaacga atatagctac cataaggaag
     3541 agaccgagga taatgtaccc cattaccggg tctctgaaga acttagaaat aaagaatacg
     3601 attacgaaga tagcaaaagc gccaaacata aagatgttca gtagattcgc gtagttctca
     3661 ctggcctccg cagacattag aaaaaccagg aacccggtca tattgtaacg cgtttcatct
     3721 attagtggtg caagcaccag cgaaacattc ctttcctcga atgtgtagtc gagggaggag
     3781 taatagtttt ccaggtaata gacgctttca agcccctttt cgaagtcctc tgaactgctg
     3841 atggattcgt agaagacttt cagctgttcg ttaggcgcat aaacctgcag ttcatcccag
     3901 ccgggaacac catttatata taatatctcg gcttctgtga actcctcgtt cacggccact
     3961 atccagtcct tcaccgtttc cacgtctctt ggtgcagatt ccgcaatgac tgtgacaagc
     4021 tgcctctggt tttctctgac aacgtcaaaa aacgagttct tcagtgtatc aaagatccag
     4081 aatagtgcaa tgaagacagc gatacttatg aacccgaacg ataagagctt tctccaggaa
     4141 tacaatttga tcacacccca agagtaacct tgattgtcga aggcagaatg aagaagagca
     4201 ggagactggc tcctgctcta atcaattact tattccgcag cgccgccgat ctctttcgtc
     4261 cagcggtcga gcagtctttc cttgttttca gcatcccaga cataatcctg atgaaccgtc
     4321 ttgatttcat ccatgctgaa tgagagcgga tgctttgggg cgatcttgga aaccggtatt
     4381 acgtaccagc ttgcgatgat tgcctgtgcc tcttcggtga gtatccagtc gtaaagcttc
     4441 ttggcattta cgggatccgg gccgcccttt atcaaagaca tagatgcaat ctcgaagccc
     4501 gtaccttcct cgggaattgt aatcgtaatg tcggcacctt cggccttaag ctttaccata
     4561 tcatgggcat aaccaatcgc gaacggaatc tctccaatcg caacgctctt accgcccgca
     4621 gagccggacc ttgtgtacat ctcgatgttc tcgtccagct tcttcagata gtggaatgcg
     4681 aggtcttcat tacgaccgaa gagggaaatc atgcaggtta tgaggttgta agccgttcct
     4741 gacgtatttg gatttgcaac ccttatcaaa cccttgtact tggaatcgat tatatcgaac
     4801 cagcctttcg ggggttctag accgagttcg gctgctcggc cattgtgagt cgcaaatgcc
     4861 agaggcccaa gataaagtcc tatccagtag ccctcagggt ccttgtactg gtccggtgtg
     4921 tttgcagcgg ctctggagaa ataaggcgtg gtgagacctt tgagcttggc gcttatatga
     4981 ttcagtccca ctcctccaac ccatatggaa gcttgtggat tcagcttttc cgcttccatt
     5041 ctcgcctcgg cctctccacc ggaaagtctt acccagtcaa cccttatgcc ggttacttcc
     5101 tcgtacttgt caaacagctg ctttgcagca agctcctcga gagtagtgta aacggtgaaa
     5161 gaatcagcgg caagaagggt taccgaaaag gcagcgcaga tgacagtaaa caaaataaga
     5221 aatcttctca ttgcaaagaa acctccttga aagatgtgtg gttttggaaa acgtttgcct
     5281 ttgttgattc aaatgaaagt tggatgaatt gtatgtcttg atgagattat accattacga
     5341 tgacttgaaa ctgccataac atttctcaac tttcttggaa aagaacggcc tgccaacgaa
     5401 ggaagaaggg acataagaaa caatgctgcc ttcggcagga agccttctca ggtccaccat
     5461 caaaggttct ccgagaaaaa taccggttct tcgttccgac gctgcgcatt caggttacag
     5521 gaaaaaccaa gatcagaagg ttgaggcggg agatttacgc gagggacaag acgttcgctg
     5581 cgctcttgag aagacgagag cgaaaatagg gactgaccgg ctcccatggt cccatttttc
     5641 gcgagggata gggatcctga ccacgagcat tgtcaggatg acgtttagcg gtgtcatccc
     5701 atgattgccc catactgcca ccccgtagtg cttctgtacg gggtcccaca cccatgagcc
     5761 gcagcgctga taaaatcaaa accggatcct gaaacaagtt cgggatgaca gagtgagaaa
     5821 cttcgaaaag ccgatgccgg gatgaccctg aacagagacc ccaaacagaa gccctgaaca
     5881 agagcatttc ag
//
LOCUS       DAOQYI010000083         5831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_14016_length_5831_cov_77.966066, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000083 DAOQYI010000000
VERSION     DAOQYI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14016_length_5831_cov_77.966066"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            560..1258
                     /locus_tag="PKL93_10650"
     CDS             560..1258
                     /locus_tag="PKL93_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ71757.1"
                     /translation="MIRLLRILLVEDDENLANGISYALTQEGWATTLAGSVSYALEML
                     ESGDFDLALLDVMLPDGNGFELCKKIRERSEMPIIFLTARDEEVNIVMGLESGADDYV
                     TKPFRLRELISRIKANARRIQKRSVEEGGSLKSGPLELNTSKLRAYRSGKEIILTPTE
                     FKILRTLMENEGIVLGRDRLLQKVWDVDGEFIDDNTLSVHIRKLREKVEEDPSKPKLI
                     ETLRGLGYRWNGGK"
     gene            1259..>1800
                     /locus_tag="PKL93_10655"
     CDS             1259..>1800
                     /locus_tag="PKL93_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNQ71758.1"
                     /translation="MLRFLFREKANRLASVAGIVISLAVVIIAVLLIWSALQEQRSRQ
                     YEREISLIGRLVSLGGLSDETFQRLLSESWPEDFEAALNLLKPYGYTEAPPESYGSTY
                     KNFRSRIITIISLSSITLVLLWVSLLSLIAKKQSDFLKETSLRLDALMSGRYDGKSIF
                     DGEGDTAILSAQLNALSRRL"
     assembly_gap    1801..1900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1901..2093
                     /locus_tag="PKL93_10660"
     CDS             <1901..2093
                     /locus_tag="PKL93_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ71759.1"
                     /translation="EDSSKIFQSFYRGSSAGRSKKGSGLGLSLAKAIIERHQGDIKLK
                     SSTERGSTFTIELPVRREE"
     gene            2187..2912
                     /locus_tag="PKL93_10665"
     CDS             2187..2912
                     /locus_tag="PKL93_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71760.1"
                     /translation="MEVLKTAGLRKVYGSGANAVTALKGAELSVEESEFIAIVGPSGS
                     GKSTLLHLLAGLDRPTAGSVHIDGKNLYTMTDTQLSIFRRRRIGFVFQFFNLIPVLTA
                     RENIELPLILDERKVDEPYLNELIRLMKLEDRIDHLPSALSGGQQQRVAIARALITKP
                     AIVFADEPTGNLDSKTSQEVMDLLKISARKFHQTLVIVTHEEGIAERADRIITIEDGE
                     IVSDYSNADVGNVFSSALGGQKV"
     gene            2909..5413
                     /locus_tag="PKL93_10670"
     CDS             2909..5413
                     /locus_tag="PKL93_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ71761.1"
                     /translation="MKRYTELSRKYLSRRLRRTLYTLLGVALAVAFITAIMITFESIE
                     ASQLQSMENSVGRYHGKVVETSESELMDLKIHVLIEKLGIQKIAGTIEFPEEKASLFV
                     EEANELTLNLRGKSIEEGRLPEKPGEIALDSFSAQLLGITPETGQMVSLDIGGKKRTY
                     EVVGIVSKARQESAMSKAFVEISPEEFSEISEESRVVKNAYFTVEAPNNGIRAAAIKV
                     ASDLKIRDKTTYNGSLIYFLENLSPVNWPAVVLGLLVAVASMVSIYNTVQISVLERIR
                     EFGLLRAAGATPAQIRKVVFRESILVSLVGIPLGLATGVLLSFAIALYAGSQLTGLGG
                     FATMITPLSLLLGAILGFLSVVVSSLIPALRAGKVSPVEAMRHYGVEPSDFEVKGIVK
                     GQSSSGEKIPLKLAKRNMRRNLRTTTISVISMTMAATLFIAFSYFAGNFDTEKIARGV
                     VKSDFSIRVASMYDDGPDEQTIEEILSFEDVQTVAAAKFITGRLLTEWEDRDLDNKSF
                     ATFSTPEAGMRATIVDNSGMCMALLAYNDLGIELMKTKVISGSIDLTRATSEPVIIID
                     IENSNRHGIKAGDRVLLKTYYLNESMVEIPVASEFVVAAVVQELPSVVGYTVEGGMLV
                     AACSEKIIEVFRPESDDVHLTMMDYMDHYSYVDIYLRRGSDAEALESTIKAIADRYRN
                     STFISYSEYKKETEDAISTLSTLVYGLIAIVGFIGICGITNTINTTIILRRREFGILR
                     AVGMTGKQLKAMLIYEGLIFGFISAVSSVVLGLILSYTVYSLLRSEMSHLNWSIPWIG
                     IVLAVAGAIGAGILTTLASSRKVTSLSITESIRTIE"
     gene            5512..>5831
                     /locus_tag="PKL93_10675"
     CDS             5512..>5831
                     /locus_tag="PKL93_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ71762.1"
                     /translation="MEIQVATISVSSLERARVFYEEILGFEPDIFYEQTNWQSYKLDG
                     TAGFGIIETPGLKRNRNSDILNFRITGVEELWNRIKDTVEIDTPLETTPYGILKFVIR
                     DPDG"
BASE COUNT         1580 a         1324 c         1491 g         1336 t
ORIGIN      
        1 agagcacact ccccaccgca agcggtcccc cccgctcaag cggggattta agaagggatc
       61 acaaaaaacg aggttagagg ttgggcgtat ggggtcggaa gagcaggaga cacttcttga
      121 ccgttgaatc gttctccgtt cgccggggag agccgtgaag caatagtatc gagattccga
      181 ccaggagctt tgtcggaatg acagaattgg gcgttttcgg aataaccata cgctgtcatc
      241 ccggacttga tccgggatct gttcctagaa cgaacaacct ggacgcgtag cgtcggaacg
      301 aggaaccgcg attctcggtg gggacggtgg acctgagaaa acttcctgcg aagcagcctc
      361 acttccccgg atgatcctcc gggcatcact tccgccggga tctttcggcg catcacttcc
      421 ggggatgctc ttcccagcat cacttctcaa tggagtcggc attgcgcccg ccgatgtttt
      481 tcagcgcttt tctatacatt tacattccat atataataga aacgccggat ctacaaggac
      541 cggtcatggc cattcggaag tgatacgatt gctcaggatt ctgctagttg aagacgacga
      601 aaaccttgcg aacggcataa gctatgcgct cacgcaggag ggctgggcaa cgaccttagc
      661 cgggagtgtg tcgtatgctc tcgaaatgct agagagcgga gactttgatc tggcgctcct
      721 agatgtaatg ctccccgacg gaaacggatt tgaactctgc aagaagatac gcgaaagatc
      781 agagatgcca ataatcttcc ttactgcaag ggacgaggaa gtgaatatcg taatgggtct
      841 cgagagcggg gcggacgatt acgtaacgaa accattcaga ctaagggagc tcatatcgcg
      901 aatcaaagcc aatgcacgga gaatccagaa gcgttccgtt gaggaaggtg gttcgctaaa
      961 gtcaggcccc ttagaactga acacctctaa gctgagggct tacagatccg ggaaagagat
     1021 catactcact ccaacggagt tcaaaatact cagaactctt atggagaacg aaggtattgt
     1081 cctcggaagg gatcggctcc ttcagaaggt atgggatgta gacggtgaat tcattgatga
     1141 caacaccctc tctgttcata tccgaaagct cagagagaag gtggaggaag atccctcaaa
     1201 gccgaagctt attgaaacgc tgagaggcct gggttacagg tggaatggag ggaagtagat
     1261 gttaagattc ctcttcagag aaaaagccaa tagactggcc tcagttgccg gcattgtgat
     1321 ctctctcgcg gtcgtcatta tcgccgtgct gctcatatgg tctgcattgc aggaacagag
     1381 aagtcgtcag tatgaaaggg agatctcgtt aattggaagg ctggtttcgc tgggtggtct
     1441 ttcggatgag acgtttcaga ggctgctttc tgagagttgg ccggaagact tcgaagctgc
     1501 gctgaatctt ctcaagccct atggatacac agaagcgcct cccgaaagct atggatctac
     1561 ttacaagaat ttccgctcca ggataattac gataatttcg ctctcttcta taactcttgt
     1621 tcttctttgg gtttcactgc tctccctcat agcgaaaaaa cagtccgact ttctgaaaga
     1681 aacatcgctg agattggacg ccctgatgag cgggagatac gatggtaaat cgattttcga
     1741 cggcgagggg gacacggcca ttctctcggc acaactgaac gcgctcagca gaagactgga
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agaagattca tcgaagatct
     1921 ttcagagttt ctaccggggc agttctgccg gcagatcgaa aaagggttca gggctcgggc
     1981 tatcgctggc taaagcgatt attgagagac accaaggcga cataaagctg aagtcatcta
     2041 cggaaagagg atcgactttc acaatagagc tccccgtaag acgagaagaa tgagggctta
     2101 accttgcgat ttcttaagct tatccttcat ctacgaataa gaaagactcc ttatactctg
     2161 aacagaaatc aataaggagg cggattatgg aagtactcaa aactgctgga ctgagaaaag
     2221 tatatggatc tggcgcgaac gcagtcactg cgctaaaagg ggccgaactt tccgtcgagg
     2281 aaagcgagtt catcgcgata gtcgggccta gtggctcggg aaagagtacg ctccttcacc
     2341 tgcttgcggg gcttgatcgg cctaccgcgg gaagcgtcca catagacggc aagaatctct
     2401 acaccatgac ggatacgcag ctttcgatct tcagaagaag aagaatcggg tttgtctttc
     2461 agtttttcaa cctgattccc gtcctgactg ccagggaaaa cattgaactc ccgctgatcc
     2521 tggacgaaag aaaggtcgac gagccctatc tgaacgaact cataaggctt atgaaactgg
     2581 aagacagaat cgatcacctc ccgtctgccc tttcaggcgg ccagcaacag agggtggcca
     2641 tagccagggc tctcataacg aaacccgcta tcgtctttgc cgacgaaccg acaggaaatc
     2701 tcgacagcaa gacgagccag gaagtcatgg acctgcttaa aataagcgcg aggaaattcc
     2761 accagactct cgtaatagtg actcatgaag agggtattgc agaaagagcc gacagaataa
     2821 tcactattga ggacggcgag atcgtttcgg actactcgaa tgcagacgtg ggcaacgtct
     2881 tttcttcggc cttgggaggt cagaaggtat gaagcgttat acagaactaa gcagaaagta
     2941 cctttcgaga cgacttagga gaacgttgta cacactcctg ggagtggccc tcgccgtagc
     3001 cttcataacc gcaatcatga tcactttcga aagcatcgaa gcttcgcagc ttcagagcat
     3061 ggagaactct gtggggagat accatggcaa agtagtagag acaagtgaat ccgagctcat
     3121 ggacttgaag atacatgttt tgattgaaaa gctgggtatt cagaaaatag ccggaacaat
     3181 tgaatttccc gaagaaaagg cttctctgtt tgttgaagag gcaaatgagt tgacgctcaa
     3241 cctgagagga aagagcatag aagaaggccg tttaccggaa aaacctggag agatagcgtt
     3301 agactcattt tcggcgcagc ttctgggaat cactccggaa accggccaaa tggtctcact
     3361 tgatattgga ggcaagaaac gtacctatga agtagtaggg atagtatcaa aggctagaca
     3421 ggagagtgcg atgtcaaagg cctttgtgga aatttcacct gaggagtttt cagagatttc
     3481 tgaagagtcg agagtggtga agaacgccta cttcaccgtt gaagcaccaa acaacggaat
     3541 aagagctgca gcgattaagg ttgcctcgga tctcaaaata agagataaga caacttacaa
     3601 cggttcgttg atctactttc ttgagaacct atctcccgtc aactggccgg ccgtcgttct
     3661 gggactgctc gtcgcagtcg cttcaatggt ctctatctat aacaccgtac agatatccgt
     3721 tctcgagaga atacgtgaat tcgggcttct cagggcggcc ggtgccactc ccgcacagat
     3781 aaggaaggtc gtcttccgag aatcgatcct cgtaagtctt gttggcattc ccttaggact
     3841 ggcgacaggc gttttgcttt ccttcgcaat agcgctctat gcgggatctc agcttacggg
     3901 gctaggcggc ttcgcaacca tgattacacc cctatcactc ttacttggag ccattctcgg
     3961 attcctgtcg gtagtcgtat cttctcttat tcccgcacta agggcgggga aagtctcgcc
     4021 cgtcgaggcg atgagacatt acggggtcga accctctgac ttcgaagtca aagggatcgt
     4081 taaagggcaa agttcgtctg gagagaagat cccgctgaaa ctggcgaaaa gaaacatgag
     4141 gagaaaccta cgcacaacga ctatttctgt tatttcaatg acgatggcgg caacactttt
     4201 catagccttc tcgtactttg ccggaaactt cgatactgag aaaattgcgc ggggcgttgt
     4261 gaagtcggat ttctcaataa gagtggcaag catgtacgat gacggacccg atgagcaaac
     4321 gatcgaagag atactctcat tcgaagatgt acagacagtt gcggctgcga agttcattac
     4381 gggaagactg ctcaccgaat gggaagaccg cgaccttgat aacaagtcat tcgcgacctt
     4441 ctccacccct gaggctggaa tgcgcgcgac aatagttgat aatagcggaa tgtgcatggc
     4501 tctcctcgct tacaacgatc tcggtatcga gctgatgaag acgaaagtca tttcgggatc
     4561 gatagatttg acgagagcaa cttccgaacc tgtaatcatt atcgacatag aaaacagcaa
     4621 caggcacgga atcaaggcag gagacagagt tctgctcaag acgtactatc tgaacgagtc
     4681 catggtagaa atcccagtcg cctcggagtt cgtcgttgcc gctgtagtgc aggagctgcc
     4741 atcagtcgtc ggctacacgg tcgagggtgg aatgcttgtt gcggcatgca gcgagaagat
     4801 aattgaggtt ttcaggcctg agagcgacga tgttcatctc accatgatgg actacatgga
     4861 ccactactcg tatgtagata tataccttcg aaggggaagc gacgctgaag ctctcgaatc
     4921 aacaatcaaa gcgatcgccg acagatacag gaactctacc ttcatctcat acagtgaata
     4981 caagaaagag actgaagacg cgattagcac actttcaacc ctagtgtacg ggcttattgc
     5041 aatagttggc ttcataggca tctgcggtat aacaaacacc ataaacacta ctattatcct
     5101 gcgtcgcaga gagtttggaa tacttagagc ggtgggcatg accggaaaac aactcaaagc
     5161 tatgcttata tacgaaggcc tgatcttcgg attcataagt gccgtatcgt ctgtcgttct
     5221 aggtctgatt ctctcttaca cggtctattc gctcttgaga tccgagatga gtcatctcaa
     5281 ctggtcgata ccatggatcg gaattgttct agcggttgcc ggcgcgatag gggcaggaat
     5341 actgaccacg ctcgcttcgt cgagaaaggt gacgtctcta agcataaccg aatcgatcag
     5401 aactatagag tgatgtagaa atgaatcttt cagtttgact gaggtcggac tctgataggt
     5461 tttatcgccg gcctcattct tcttaaaaca aattaatatt gggaggctta aatggaaatt
     5521 caagttgcta ctatatctgt tagctctctt gagagagcaa gagtcttcta cgaagagatc
     5581 ctcgggttcg aaccggatat tttttacgaa cagaccaact ggcagtccta taaactcgat
     5641 ggaacggccg gctttgggat aatcgaaact ccgggattga aaagaaacag gaattcagac
     5701 atcttgaact tccgaatcac cggtgtcgag gagctgtgga atagaatcaa agacactgtt
     5761 gagatcgata ctcctctcga aacgactccc tatggaatcc ttaaattcgt catcagagat
     5821 ccggacggtt t
//
LOCUS       DAOQYI010000084         5815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_14081_length_5815_cov_77.485069, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000084 DAOQYI010000000
VERSION     DAOQYI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14081_length_5815_cov_77.485069"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1970)
                     /locus_tag="PKL93_10680"
     CDS             complement(<1..1970)
                     /locus_tag="PKL93_10680"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ71763.1"
                     /translation="MNERVREQLTELVKTHGVDILDQPDKIETLLKTSFGESETEIMA
                     IVTALREGLLRELLRSEDSSTQTAAIEGFSRRLRKNCSMDEEAATWAIESIALALGKL
                     TPAETESSGMKTTNGDELVYVEGGSFVMGDTWGDGADVERPAHRVNITYDLYVGKYPV
                     TFEEYDRFCMETGATEPEDEGLGRGRRPVINVNWFQATWYCNWLSEKEGLPPAYYEDG
                     SLLDENGKKTNDIARVRGYRLLSEAEWEYAARGGRESRGHKYSGSDDPSLVAWFDEKP
                     SEAPTDLSKAKRLVAHEVGMKMPNELGLYDMSGNVWEWCSDRFDDYAGSEQTNPHSIS
                     EGGRVIRGGCWFRPAELTRIAHREELSPMEIHNGVSFRICRAADRKTKADSFSQINER
                     QADESPFIPAGEIPDLVLVERGSFKMGDTWGDGLIDERPVHEVSIMYDFYIGKHEVRF
                     SEFDVFCEVTGRIPPSDEDWGRGDLPVINVTWWDAVEFCNWLSEREGLPKAYDDDGRL
                     LDATGKATDKLERVPGFRLPTEAEWEYAARGGNKSKGHKYAGSDDVDEVAWYSENSGI
                     KTHEVGKKAPNELGIHDMSGNVWEWCSDWYEEDYYGYSPNVNPYNTEGDYERVMRGGS
                     VGLNESFARVTHRDYLEPSSAGNAVGFRICRTA"
     gene            complement(2068..4965)
                     /locus_tag="PKL93_10685"
     CDS             complement(2068..4965)
                     /locus_tag="PKL93_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ71764.1"
                     /translation="MNKNTFISASAGTGKTYRLVEEYMEILHSDEKLGIENILAITFT
                     EKAAAEMKERVAKAILEQIDKAASESERKRWKEINSRVMHAWISTIHSFCARILRESI
                     LNTNSDLDPGFSIITGTKKILELQALGRFFSRNLDSLDEMIDIYGLDKTYKILEDCLS
                     KQRHNMYLYSPIENKDKEENAEKISRATAVCHEAFKKLNKEYEELTLKKAQLDFEQLL
                     EETRKMLNDNPKIAAEYSKRFKYILVDEFQDTDELQAEIISKLTASGNVKVLFVGDDK
                     QSIYRFRGANVEIFNLTRRKFEESDDATDTLGKSYRSHPEIVKFHNIFFKKVMSNPTL
                     EAFASRYGKNVEALEPHGDKREKRVRILESKDKDHAGPVAAHIRSLLDEELDFREGES
                     VVTRKIRPGDIAILLRKFKNVEKYQEALEANRIPYYTVGSRKFFQRPEIAGLIAFVSF
                     LGNPADDRAFLSFFLSPAWGGTLQDAARLKEKYSHFYDCIMRSEDESFVDLRTIITRY
                     SRLARLLKPGEILDSFVGETDYLPKMTLLAMPEQCLANVIKFLQISKELDSLGISLRE
                     FSKNIKLYSEEADEGEAALENEKSDSVKIMTVHQAKGLEFPVVIVAEMTENPRKETPS
                     LLFDTASKGFTLYSKELTTDELYCVYRADLRKQVEEEKRTLYVALTRPREILVMNVDK
                     EAMEKELKKKRDDTDIGWLPQIVIGLYNDETGQLEKELEDLVTFVKAPTLLESAEIGR
                     EGTAWKEPDKDYMKRFDHLKFRRIVSPTTLDFDDKQTEILEIDSIERTSRSSTAEGLY
                     VHRIFEQLGNPAGRPKGKRLSEILKDSELLRESAAPERAKEILEQLKELGHHEIIREI
                     EDSEVCRSEVRFERAFDRFILAGVIDKLYKTSEGWKIVDFKLAEKRADMLERYKFQME
                     FYLYLLKNSLDPVSATILYLKDGDTETVTLQNTRDFEIRLENHVTDQTEEN"
     gene            complement(4958..>5815)
                     /locus_tag="PKL93_10690"
     CDS             complement(4958..>5815)
                     /locus_tag="PKL93_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ71765.1"
                     /translation="SFSTVGTYCKCKFKYFLNYVLGLQPVPEEILELSHMDAGSVFHA
                     VLSDHLGSGVPLDESLEKNLRRFSGGINKALFKRRYDQMLKILTEYVDYDQSEETSAG
                     RTVFRTELSFGFDTSDYGPLKLTPEICVHGIVDRIDTCEDGTLFVIDYKSGKSLGSAM
                     AEQLILYSMAVENAFSEENKVVSGGEFRLIKGKNKRNNFSIDYSSGERKWKFFGGLKF
                     NICDTAEKGKISDDEFIGAVEEIIKSIQSGDFSIETWKGKASSCYGCENEGIFNILKW
                     RQKGGPSDE"
BASE COUNT         1199 a         1645 c         1275 g         1696 t
ORIGIN      
        1 gctgttctgc atatgcgaaa gcctacggcg ttgcccgctg aagagggctc cagataatcg
       61 cgatgagtta cccgggcgaa actctcattt aggccaacgc tgccgccccg catgacccgt
      121 tcgtagtcgc cttcagtatt gtacgggttt acattggggc tgtatccata atagtcttcc
      181 tcgtaccaat cggagcacca ctcccagacg ttgccggaca tatcgtggat gcccagctca
      241 ttcggcgcct tctttcccac ttcgtgcgtc ttgattcccg aattttcgga gtaccaggcg
      301 acttcgtcca cgtcgtcgct tcctgcatac ttgtgacctt tgctcttgtt ccctcccctg
      361 gccgcatatt cccattcggc ttccgtaggc agcctgaatc cgggtactct ttccaatttg
      421 tccgtagcct ttcccgtggc atccagcagg cgtccgtcat cgtcataggc ctttgggaga
      481 ccttctctct cgctaagcca gttgcagaac tccacggcgt cccaccaagt tacgtttatg
      541 acgggcaaat ctcccctccc ccagtcctcg tcggaaggag ggattcttcc cgtgacttca
      601 cagaagacgt cgaattcgga gaacctgact tcgtgcttgc caatgtaaaa gtcatacatt
      661 attgagactt cgtgaaccgg tctttcgtct atcaagccgt cgccccatgt gtctcccatt
      721 ttgaagcttc ctctctcgac gagcaccaag tcgggaatct ctccggctgg aataaatggg
      781 gactcatcgg cctgtctttc atttatctgt gaaaatgaat ctgcttttgt ttttcgatct
      841 gccgctctgc agattcgaaa gctcacgcca ttgtggattt ccatcggtga aagttcttct
      901 cggtgagcaa tccttgttaa ctcggcaggc ctgaaccagc atccgccccg aatgacccgg
      961 ccgccttcgc ttattgaatg tggatttgtc tgttccgaac cggcataatc atcgaatcga
     1021 tctgaacacc actcccaaac gttgccgctc atgtcgtaca ggccaagttc atttggcatc
     1081 ttcatcccaa cttcgtgggc tacgagcctc tttgctttac tcaaatcggt cggagcttca
     1141 ctgggttttt catcgaacca ggcaacaagg ctcgggtcat cggagccgga atatttgtga
     1201 cctcggctct ctctcccgcc ccttgccgca tactcccatt cggcctcgct caacaacctg
     1261 tatccccgta ctctcgctat gtcattcgtc ttcttcccgt tttcgtccag aagactcccg
     1321 tcttcatagt aggccggagg taagccctcc ttctcgctta accagttgca gtaccaggtc
     1381 gcctggaacc agttcacgtt gattaccggt cttcttcccc ttcccagtcc ctcatcttcc
     1441 ggttccgttg ctcctgtctc catgcaaaac ctgtcgtatt cctcgaaggt cactggatac
     1501 ttacccacgt agagatcgta agtgatattc actctgtgcg ccggtctttc aacgtcggct
     1561 ccgtctcccc aggtgtcacc catgacaaaa ctaccgccct cgacatagac cagttcatct
     1621 ccatttgttg tcttcatccc ggaagattca gtttcggccg gcgttaactt cccaagcgct
     1681 aacgctatgc tttctatagc ccaggtcgcg gcctcttcat ccatggagca gttttttcta
     1741 agtcgtcggg aaaaaccttc aattgcagca gtctgtgtag aagagtcttc gcttctcagt
     1801 aactctctga gcaaaccctc tctcagagcc gttacgatag ccattatctc ggtttcagat
     1861 tccccaaagg acgttttgag caaggtctcg atcttatccg gttgatcgag tatatctaca
     1921 ccgtgcgtct tcaccagctc tgtgagttgt tctctaactc tttcgttcaa agagctcact
     1981 ccttctagac ggagactcac tgcgcaggca ataaagccct ccgtcatcaa gtctgttcca
     2041 tacagcaaag ccaatcagag actgttttca gttttcttct gtttgatcgg tcacatggtt
     2101 ttcgagtctg atttcgaagt cgcgagtgtt ttggagagtc acggtctccg tatccccgtc
     2161 tttgagatac agtatagtcg ccgaaacagg gtcgagagaa ttcttcagaa gatacaggta
     2221 gaactccatc tggaacttgt atctctcaag catgtcagct ctcttttcgg ccagcttgaa
     2281 gtcgacgatc ttccagcctt cagaagtctt gtagagtttg tcgattacgc ccgccagtat
     2341 gaacctgtcg aaggctctct caaagcgcac ttcgcttctg caaacctctg agtcctcgat
     2401 ttcgcgaatt atctcgtgat ggccaagctc cttcagttgc tccaggatct cctttgctct
     2461 ttcaggggcc gccgactctc ttagaagttc agagtccttc aggatctcac tcagtctctt
     2521 tccctttggt cttccagcgg ggttgccgag ctgctcgaag atcctgtgaa cgtacagccc
     2581 ttcggctgtt gaggaacgtg aagttctttc gatactgtcg atctccagaa tctctgtctg
     2641 tttgtcatcg aagtcgagcg tagtgggcga gacgattctc ctgaatttga gatgatcgaa
     2701 gcgcttcatg taatctttat cgggctcttt ccacgcggtt ccttcacgtc ctatttccgc
     2761 gctttcaaga agagtcgggg ccttcacaaa agtaaccagg tcctcgagct ctttctccag
     2821 ctgcccggtt tcatcgttgt aaaggccaat cactatctga ggaagccagc cgatatccgt
     2881 atcgtctctc ttctttttca gctccttctc catggcctct ttgtcgacat tcatcacaag
     2941 tatttcccgt ggccttgtaa gcgcaacata gagggttctc ttctcttctt cgacctgttt
     3001 tctcagatct gctctataca cacaatacag ttcatccgtc gttagttcct tgctgtaaag
     3061 agtgaagccc ttcgaagctg tgtcgaaaag caatgaaggg gtctctttcc tcggattctc
     3121 cgtcatctct gcaacgatca caacgggaaa ttccagcccc tttgcctggt ggactgtcat
     3181 tatcttcacg ctgtcgctct tctcgttctc cagggccgct tcgccctcgt ccgcctcttc
     3241 ggaatacaat ttgatgttct tcgaaaactc cctcaaactt atcccgagag agtcgagctc
     3301 cttggatatt tgaaggaatt tgatcacgtt ggccagacac tgctcgggca ttgcaaggag
     3361 cgtcatcttc ggaaggtagt ccgtctctcc gacgaaggag tcgagaatct cgcccggctt
     3421 cagaagtctc gcaaggcgcg aataccttgt tatgatcgtg cgaagatcga cgaaggattc
     3481 atcttcgctc ctcatgatgc aatcatagaa gtgactgtat ttctccttga gtctcgccgc
     3541 atcctgaagc gtgcctcccc atgccggaga aaggaagaac gacaggaagg ccctgtcgtc
     3601 tgcgggattg ccgagaaaac tgacgaatgc tatcagtccg gctatctcgg gccgctggaa
     3661 gaactttcta gagcccaccg tatagtaagg aatccgattt gcttcaagcg cctcctgata
     3721 cttttcaacg ttcttgaact tcctgaggag tatggctatg tcccctggcc gtatcttgcg
     3781 cgttacaaca gattctccct ctctgaaatc gagctcctca tccagaagcg aacgtatgtg
     3841 agcagcgacg ggtcctgcgt ggtctttatc tttgctttca agtattctta cccgcttttc
     3901 tctcttgtca ccgtgaggct caagggcctc cacattcttg ccgtacctcg acgcaaatgc
     3961 ctcaagcgtt gggttgctca tcactttctt gaagaatatg ttgtggaact tcacaatctc
     4021 cgggtgcgac ctgtatgatt ttccgagagt gtcagtagcg tcgtcgcttt cttcgaattt
     4081 ccttcttgtc agattgaaga tctcgacatt cgctcccctg aaccggtaaa tcgactgctt
     4141 gtcgtccccg acaaaaagca ccttcacatt tcccgaagcc gtcaacttac tgataatctc
     4201 cgcctgaagc tcgtccgtat cctggaactc gtctacgaga atgtacttga acctcttgga
     4261 atattccgct gctattttgg gattgtcgtt cagcatcttc cttgtctctt ccagcaactg
     4321 ctcgaagtcg agctgagcct tcttcaatgt cagttcctcg tattccttgt ttagcttctt
     4381 gaatgcttcg tggcagacag ctgtcgctct ggagatcttc tcggcgttct cttccttatc
     4441 tttgttttca atggggctgt agagatacat gttgtgtctc tgtttggaga ggcagtcctc
     4501 gagaatcttg taagtcttgt ccaggccgta gatgtctatc atttcatcga gagaatcgag
     4561 attgcgcgag aagaatcttc caagggcctg aagttcgagt atcttcttcg ttcccgtaat
     4621 gatcgaaaat ccaggatcca gatcggaatt cgtattcaga atagactccc tgagtattct
     4681 cgcgcagaag gagtggatag tagagatcca ggcgtgcatg acccttgaat tgatctcttt
     4741 ccatcttttc cgctcgcttt cgctggcagc tttatctatt tgttccagta tcgccttcgc
     4801 cactctctcc ttcatctccg cagctgcctt ttcagtgaag gtaatcgcca ggatgttttc
     4861 gatgcccagc ttttcgtcag agtgaagaat ttccatgtat tcctccacca gcctgtatgt
     4921 cttgccggtg cctgcagaag cggagatgaa tgtgttctta ttcatcgctt ggcccgcctt
     4981 tttgtctcca cttcagaatg ttgaagattc cttcgttttc gcacccgtag caactagatg
     5041 cttttccttt ccaggtttct atagagaagt cgcccgactg gatgcttttg attatctctt
     5101 cgacggctcc gataaactcg tcgtccgaaa tctttccctt ttcggcagtg tcgcaaatat
     5161 tgaacttgag tccgccaaag aacttccact ttctctcacc cgaagagtaa tcgatcgaaa
     5221 aattgttgcg tttattctta ccctttatga gcctgaattc tcccccgctc accactttgt
     5281 tctcttccga aaaggcgttt tcgactgcca tcgaatagag tataagctgt tcggccatcg
     5341 ctgatcccag acttttgccg ctcttgtagt cgatgacaaa tagcgtaccg tcttcacaag
     5401 tatcgatcct gtctacgatt ccgtgtacac aaatctcggg ggtcagcttc agcgggccgt
     5461 aatcggatgt atcgaaccca aaggagagct ccgtacggaa tacagtcctt ccggcactgg
     5521 tctcctccga ttgatcgtaa tccacatatt ctgtaagtat cttcaacatc tgatcgtagc
     5581 gccttttgaa gagcgccttg tttatgccgc cagaaaacct ccggagattc ttctcgaggc
     5641 tctcatctag cgggacgcca cttccgaggt gatcgcttag aacggcatga aaaacgctcc
     5701 ccgcatccat gtgagaaagc tccagtatct cctccggaac aggctgcaac ccaagcacat
     5761 agttcaagaa atacttgaat ttgcacttgc agtaggtccc gaccgtagag aagct
//
LOCUS       DAOQYI010000085         5509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_15051_length_5509_cov_67.022919, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000085 DAOQYI010000000
VERSION     DAOQYI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15051_length_5509_cov_67.022919"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2504)
                     /locus_tag="PKL93_10695"
     CDS             complement(<1..2504)
                     /locus_tag="PKL93_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNQ71766.1"
                     /translation="MSTALKSKYDWFGVLKMSGLLVSEVIYRDYFEKTPGATRDELFK
                     LRRFLDRFSSESYTVNDLVKALLFDLLGHDPNKWKYGREASAYSAKEIDHDEHLTPDF
                     VLLSEDKKTPFFIIKTLDKYTELDKRKPVGAGTWRASEHIKFETLLKLKGCPLGMLTN
                     GNEFRLFFVEEGLSAAYISWKQSDWLEEESTFEAFRNLLNANSLVSNKKKGLYNIVKE
                     SHEKQAEVTDALADQVYLVINRILDYINQSHIDIEPDKIYEAVIKVVMRLVFILYAEE
                     NSLLPHGFPEYDDNYGLIHLANSLESERAFLGDSDFESVSSYRLDAWYRILALFNLIY
                     FGSDHRELKITAHGGDLFSPDGILPLLKRIKLNNKGVRDILNDLLYMKGLRLSYAAID
                     VEQIGYVYEGLLGYTVSRASEPLVVTAKEKFVLPLSELEKRSPKEIKALIKMVDSKIS
                     RVPDSFPESGDDSVQSRVARYAEILAPEAERLIPEGAYYMSSSGARKSTGSYYTPKQL
                     TTYLVKETLGPLCTEEDTEKLKSSSEILSLKIVDPAMGSGAFLVQATNFVAEKLLKAW
                     EIEGKHTEIPDLDREILARRNVVEHCIYGVDINPTAVELAKMSLWLNTFSKDKPFTFL
                     DHKLKCGNSLVGVFHGWEDTSVIPPEVYKASGNASNEWKAKLNGLKRINSQYRPTRRD
                     SDLRRQHSMFSLTDSPDNEFARITSELAKVSHQIDVAGRETEDYFAKEQVYKEQFLRN
                     RDYEIEKLKADVWVAQWFANEIDGVLPLTTGELQELYQGIDKNQDNRSALMEVSEHLN
                     WTERISEEQRFFHWKLEFPDVFEAGGFDAVIGNPPW"
     gene            complement(2501..>5509)
                     /gene="drmD"
                     /locus_tag="PKL93_10700"
     CDS             complement(2501..>5509)
                     /gene="drmD"
                     /locus_tag="PKL93_10700"
                     /inference="COORDINATES: protein motif:HMM:NF038317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system SNF2-like helicase DrmD"
                     /protein_id="HNQ71767.1"
                     /translation="EAGRMARVRDRLWLIDDVSFPDDVVSNSSTLVTLESLEDLDQGN
                     KMTVVLEREVDFEVLPENTLPDIGNSLDSPTVFDSFVNALRWTNKSILGGEVIRAPFY
                     GSIQQQNFQLEPVVKALSMPRISLLIADDVGLGKTIEAGMIIQELLLRGKARRILLVC
                     PASLQIQWQWEMESKFGLDFKIVDSSQITKFKREYGINVNPWLAHPRIIASMDFIKME
                     GRKTEFLRSCEVNSSKYIRSWDLLVVDEAHNAMPQPTKKYYRDSDRTKMLNEIGRHFE
                     HKIFLTATPHNGRKESFVAFLDMLDPLSFNRGEDPAVNKENFKKRLDRIMVRRLKRGP
                     TGVKDSLGRAMFAGREIVSLDIDPSEDEKKSLSLLNQYIRQRVSGGGNGENNRPLEFA
                     MTILKKRLLSSPRAFYNSIVVHTQHLKDTKDSPLYLKLIVDRLDEDWDNDVVREQTEQ
                     ELISATATDNGKNYLSELVSVASRLRNKPDSKVKELIKWINNNLRDNGRWNRERVVIF
                     TEFKDTMEYITDQLIENGVEEERILQLYGGMEMGKREAIKALFEADPDEAPARILVAT
                     DAASEGINLQQHCRYMFHMEIPWNPIRLEQRNGRIDRHGQTRDVTIYHPVYENNSDSQ
                     FLKTIVRKVEQIREDLTTMNPVIADKIQKQMLGLEKMNEGFLDSMRDDLIKDLMDVRG
                     EAANLGQKIAASREKLNLTPEAVRELLDSAIKLNGVASGVRHVEGSLYKIERLPDSWK
                     RLQKYVMQDGKTRLLSFEPTTGGKAQHLHPNHPLVKRAITYFRANIWSRKIEGTDQKL
                     NKVTCKVVPSGKMAVPHVLLYVRAMAVNKLSQPLVEEVGVIGGSFDQGSYLPVSDSTY
                     LHYLDSLGAFDQRSAPLVLELKRLMKVNEKAILDNVNSFKKSWKERLNKELKEVTSSE
                     KKTLIEMIKDRTKEIEKTISRLNRYEGSMFEDQMQYSEDIRLLKNRQIYLKSQLDEIP
                     GKIEDKYTLKGDPLVNVVALCFLVPEEMIK"
BASE COUNT         1244 a         1371 c         1224 g         1670 t
ORIGIN      
        1 tcccaaggcg gattgccaat gaccgcatcg aaacctccag cctcaaaaac atctggaaac
       61 tcaagtttcc agtggaagaa acgttgctct tcacttatcc tttctgtcca gtttaagtgt
      121 tcggatactt ccataagagc cgatctgtta tcctggtttt tgtctattcc ctggtagagt
      181 tcctgaagtt ctccggttgt caatggaagg actccgtcaa tttcatttgc gaaccactga
      241 gctacccaaa catcagcctt aagcttctct atttcgtaat ctcggttcct aagaaactgt
      301 tctttgtaaa cctgttcctt ggcaaaataa tcttctgttt ctctaccggc gacgtctatc
      361 tggtgtgaga cttttgcgag ctcactcgtt attcttgcaa actcattatc cggagagtct
      421 gtaagtgaga acatagagtg ttgccttctt agatcactat ccctcctagt gggtctgtac
      481 tgagagttta ttcttttcaa tccatttaat ttggctttcc attcattact ggcgttaccc
      541 gaagctttgt aaacctcagg aggtataaca ctggtatcct cccagccgtg aaagactccc
      601 acaagagaat taccacactt cagcttatga tccaggaacg tgaatggctt atccttggaa
      661 aaagtgttca accagagaga catcttcgcc agttccactg ccgtcgggtt tatgtccacc
      721 ccgtatatgc agtgttctac gacgttcctt cgtgcgagga tctctctatc taaatccgga
      781 atctcggtgt gtttgccctc gatttcccag gccttcaaga gcttctccgc aacgaaattc
      841 gttgcttgta ctagaaaggc tcctgaaccc atagcaggat cgacaatctt cagagaaagg
      901 atctcggaag agctcttgag cttttctgtg tcttcttctg tacatagagg ccccaatgtt
      961 tcctttacga gataggtcgt cagttgtttc ggtgtgtaat aactcccggt tgactttctg
     1021 gcgccggaag aagacatata gtatgctccc tcgggaatga gccgctcggc ttcaggggcc
     1081 aggatctcgg cgtaacgcgc gactcttgat tgaactgaat catccccaga ttccgggaag
     1141 gagtctggta ctcggcttat cttcgaatcg accattttga taagtgcctt tatctctttt
     1201 ggagagcgtt tctcaagctc actaagagga aggacgaact tctcctttgc ggtaacaaca
     1261 agtggctcac ttgcacggga aacggtgtaa cccaggaggc cctcgtagac ataacctatt
     1321 tgctcaacat ctatggcagc gtagctaagc ctgaggcctt tcatatacag aagatcgttg
     1381 agaatgtccc ttacgccctt gttgttcagt tttattctct tgagaagggg aagaatcccg
     1441 tcaggtgaga acaagtcccc gccgtgagcc gttatcttca gttctctgtg atctgagccg
     1501 aagtagatca gattgaaaag agcaagtatc ctgtaccagg cgtccagtct gtatgaggaa
     1561 accgactcga agtcgctgtc tcccaagaaa gctctttcag actcaagtga atttgccaga
     1621 tgaatgaggc cgtagttatc atcgtactca gggaatccgt gaggcagaag ggagttctcc
     1681 tcggcgtaca gtatgaagac cagccgcata acgaccttta ttactgcctc gtatatcttg
     1741 tccggttcaa tatctatgtg gctctggttt atgtaatcca gtatcctgtt tattaccaaa
     1801 taaacctgat cggccagggc gtcagtaacc tctgcctgtt tctcgtggct ttccttgact
     1861 atgttgtaaa gacccttctt cttgtttgaa acaagactat tcgcgttgag aaggtttctg
     1921 aaggcctcga acgtagactc ctcttccagc cagtcgctct gtttccagga gatgtaggca
     1981 gcagagagac cttcctcgac gaagaagagc ctgaactcat taccgttggt tagcatacct
     2041 agaggacaac cttttagctt gagcagcgtc tcgaatttta tatgttcact cgcccgccag
     2101 gtgccagcgc caactggttt tctcttatcc agttcggtgt atttgtccag tgtctttatt
     2161 atgaagaatg gtgtcttctt atcctcagat aaaagaacaa aatcgggggt caggtgctca
     2221 tcgtgatcaa tctctttagc cgagtaagcg gatgcctctc ttccatactt ccatttgtta
     2281 ggatcgtggc cgagcagatc aaagagaagg gccttgacga gatcgtttac cgtatagctt
     2341 tcactgctga agcggtcaag aaatctccga agcttgaaga gttcgtcacg ggttgcaccg
     2401 ggggttttct cgaagtaatc acggtagatc acctcggata ccagtaagcc cgacatctta
     2461 agaacgccga accagtcgta tttactcttc aatgcagtgc tcatttgatc atctcctcgg
     2521 ggacgagaaa gcagagtgca acaacattga cgagaggatc acccttgagt gtgtatttgt
     2581 cctcgatttt tcccgggatc tcatctagct gggatttaag gtatatctgt ctgttcttca
     2641 gaaggcggat gtcttctgaa tactgcatct ggtcttcgaa catagatcct tcgtatctgt
     2701 tcagcctgct tatagtcttc tcgatctctt tagttcgatc ttttatcatc tcgataagtg
     2761 tcttcttctc actggaggtg acttctttca attccttatt gagtctttct ttccagcttt
     2821 tcttgaacga gttgacatta tccagtatcg ctttctcgtt gactttcatt aaccttttca
     2881 gctcgagaac gagcggggct gacctctgat cgaatgctcc aagagaatct agataatgaa
     2941 ggtaagtgct gtcagagacg ggaagataac taccctgatc gaaactccca ccaatgacgc
     3001 ccacttcttc aacgagcggc tgagagagtt tgttgaccgc catcgccctc acatacagaa
     3061 gtacgtgagg aacagccatc ttccctgaag gaaccacctt gcaggtcacc ttgttcaatt
     3121 tctgatcagt accttcgatc tttctcgacc aaatgttggc tctaaagtac gttatggccc
     3181 tcttgacgag tggatggttg ggatgaaggt gctgggcctt gcccccagtt gtcggttcaa
     3241 aggaaaggag ccttgtcttg ccgtcctgca tcacatactt ctgaagtctt ttccagctgt
     3301 cagggagtct ttctattttg taaaggctgc cttccacgtg tctaactccc gaagctacac
     3361 cattcagttt tattgcgcta tccaataact ccctcacagc ctcaggagtg aggttcagtt
     3421 tctctctcga ggcggctatc ttctgcccga gatttgccgc ttcgcctcta acatccataa
     3481 ggtcttttat gagatcgtct ctcattgaat ccaggaaccc ttcattcatt ttctccaggc
     3541 cgagcatttg cttctgaatc ttatccgcta tcacagggtt catcgtggtc aaatcttcgc
     3601 ggatctgctc tacttttctc acgattgtct tcaggaactg gctatccgaa ttgttctcat
     3661 aaacaggatg ataaatggta acgtctcgag tctgcccgtg cctgtctatt cggccgtttc
     3721 tttgctcaag tcttattgga ttccagggga tctccatatg gaacatataa cggcagtgct
     3781 gttgtaggtt tatcccctcc gaggcagcat ccgttgcgac gagtattctt gcgggtgctt
     3841 cgtctgggtc ggcttcgaac agtgccttta ttgcctccct ttttcccatc tccattccgc
     3901 cgtagagctg aaggattctc tcctcttcaa caccgttctc gatcaactgg tccgtgatgt
     3961 actccatcgt gtctttgaac tcggtgaata taaccactct ttcccggttc catctaccgt
     4021 tatctctaag gttgttgttt atccacttaa taagctcttt gactttactg tcgggtttgt
     4081 ttcttaggcg agaggctacg cttaccagct cgctaaggta gttctttccg ttgtctgtag
     4141 cagtcgcgct tatgagttcc tgctccgtct gttctctaac cacgtcgtta tcccagtcct
     4201 catccagtct atccacaata agctttaggt atagggggga gtctttcgta tctttcagat
     4261 gttgtgtatg cacgactatg gagttataga aggctctggg tgaagagaga agcctcttct
     4321 taagaatcgt catagcaaac tcgagaggtc tgttattctc gccgtttcct ccgccactaa
     4381 ccctttggcg tatgtattga tttagtaggc tcaacgactt cttttcatcc tctgaaggat
     4441 caatgtctaa ggaaacaatc tctctgcctg caaacattgc gcggcctaga gagtccttaa
     4501 cgccggtggg tcctcgcttc aatctcctta ccattattcg atccagcctt ttcttgaagt
     4561 tttctttgtt gactgctgga tcttctcccc tgttgaagct gagcggatcg agcatatcca
     4621 gaaaggcgac gaaggattcc tttcgaccgt tgtggggagt tgcggtaagg aatatcttgt
     4681 gctcgaagtg ccttccaatc tcattcagca tcttggttct atccgaatcg cgatagtact
     4741 ttttcgtcgg ttgaggcata gcgttgtggg cctcatctac taccagaagg tcccaggagc
     4801 ggatgtactt gcttgaattg acttcacagg aacgaagaaa ctctgtcttc cttccctcca
     4861 tcttgataaa gtccattgag gctattatcc tggggtgagc gagccaggga tttacgttta
     4921 tcccgtattc tctcttgaat ttagttatct ggctgctatc cactattttg aagtcaagac
     4981 cgaacttgct ctccatttcc cattgccact gtatctgcaa ggacgcgggg cacaccagga
     5041 gtatccttct tgcctttcct ctcagtagaa gttcctggat gatcataccg gcttctattg
     5101 ttttgcccag cccgacatcg tctgctatta gaaggcttat tctcggcata gacagagcct
     5161 tgactacggg ttcgagctga aagttttgct gctgtatgga gccgtagaat ggggctctaa
     5221 taacttcccc accaaggatg ctcttgttcg tccatctaag ggcatttaca aagctgtcga
     5281 ataccgtagg agagtcaagg gagttaccaa tgtcagggag tgtgttttcc ggaagaactt
     5341 cgaagtcaac ttctctttcg agaacaacag tcattttgtt tccttgatcc agatcctcta
     5401 agctttccaa tgttacaaga gtcgaggaat ttgacacaac atcgtctgga aaggaaacgt
     5461 catctattag ccagagacgg tcccgtaccc gggccattct cccggcttc
//
LOCUS       DAOQYI010000086         5396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_15467_length_5396_cov_50.522936, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000086 DAOQYI010000000
VERSION     DAOQYI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15467_length_5396_cov_50.522936"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..911
                     /locus_tag="PKL93_10705"
     CDS             423..911
                     /locus_tag="PKL93_10705"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNQ71768.1"
                     /translation="MKLKRVITICLLLFVLISIAYFAYDEIKLISQSSKSRESILEEQ
                     DDPFLPEDFVVLYYFHGTIRCKVCLDMEANAKKAVETHFSSQYGTGTLRWEVVNIDLP
                     ENYHYLEDYTIMYNTLVIQQYIDGKPGEWKELWHAWDLSEEEDQYIEYVRDEVASFLG
                     VN"
     gene            911..1594
                     /locus_tag="PKL93_10710"
     CDS             911..1594
                     /locus_tag="PKL93_10710"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNQ71769.1"
                     /translation="MDNFAFSMISALWFGILTSISPCPLATNIAAVSFISRNIGSPRK
                     SLLAGLLYTLGRVVTYVAIGAILVSSLTAAPGLSRFLQKYMGKLIGPIMIVVGMFLLE
                     LLSFRNSGRNLDTTSQEKIKSKGLLGALILGSLFALTFCPVSAALFFGSVIPLAVNEQ
                     SRLMIPAIYGIGTGLPVVVVAIGIAISTKLVAQMFRRISRFEFWARTITGAVFIVLGI
                     YLSIRRIFL"
     gene            complement(1655..2005)
                     /locus_tag="PKL93_10715"
     CDS             complement(1655..2005)
                     /locus_tag="PKL93_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HNQ71770.1"
                     /translation="MLRYIVKILVSSVIIVLVSEVSKKSGYIGGLIASLPLTSMLALF
                     WLYNDTNSSSKVAELSTGILLFVLPSLIFFVAMPLLLRRGINFYVALALSSGVMMAGY
                     AFFSLILSKFGVRL"
     gene            complement(2111..3289)
                     /locus_tag="PKL93_10720"
     CDS             complement(2111..3289)
                     /locus_tag="PKL93_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ71771.1"
                     /translation="MHNKRLPLLLTSLLVFFVMFGFGLLLQLKLRELGASLLMVGLLT
                     TVRGAVETFGSPAWGAISDGLKKRKLLMILLVLTPALLYFAYSVIEIPIVFILFSSLI
                     AFFTAGFEPIAMALSTEHSRDSVRNTSRELSILNTANSTGMLTGRIMLSILLIFFTVS
                     QAINWYAFVALIAVIPVLFLNEEEHLVSRRKGFLNRLFPLKEDSTPLWENGLWAVYAG
                     TFLRQLGTAGATSIIAIYMTERIGLSGAATAIITSINPFMQIFSHIFFGRVIYKTGPR
                     KSTLIGIGLTIFTMVFFAVAKSWVLIAIGYFCLGIAFGAFINGAGTMISLSSPPERRA
                     EFLGLLRSARAIGFMVGPLLAGTVAEYSYFGMFIMMASLIATGGLIVIVFTKDRFITS
                     "
     gene            complement(3435..4385)
                     /locus_tag="PKL93_10725"
     CDS             complement(3435..4385)
                     /locus_tag="PKL93_10725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71772.1"
                     /translation="MKTVSLISPIGGIMIEDFFVDKPSSEESKGIMEKMYHSIPESSN
                     GRYSLSELRPIEEVYFDFLKRGDKLLVLRGQGEIIGHVHFGPQRDERFSSSLSGHIYD
                     LYVKKEHRGEGAGRTLLRSALKELKPFFQKITGNTYRFGTGWHMLKSEGFSEKRITLS
                     TNKRLADSRTNAFEIFRGLPEDLGKVFVEISSSPSLVENQLQMTSSELFFNLNEIMDS
                     GGRILICRQDEETVGVLIYSVFEDIMTYSLLGSIEFVFVSKRFRRMGIGRNLLSFLNE
                     YLSISGVEESFFECAFESDYHLWSEAIGMKEFSVLLEKKL"
     gene            4432..5325
                     /locus_tag="PKL93_10730"
     CDS             4432..5325
                     /locus_tag="PKL93_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-binding protein"
                     /protein_id="HNQ71773.1"
                     /translation="MKRISLFLVLLVFSVIALGQSEFMIPMSTEDFGLRGPVKSAKIL
                     YPENEMDLGVDEEFFLSGYSVLSFNENGSLVSQIEYDKDGNEQSAILFNYDANGKLLS
                     VSGRENGVEENVDEIKVENGRIAGIIVEDSEGDSNLVMEYDEAGRLVAQKISGMSEGQ
                     EIEMTISMKYDDNGNLVEESFGMMGMVLTKTIFEYNDKNQSVRELEYMYMFAEPGTEP
                     EPIVSTLEYNEMGDVVLRVSDSSFGEEKEAVVYEYEYDSAGNYTHKVAYYVMDIAELQ
                     NETWKETAMIEEEETREIEYY"
BASE COUNT         1605 a         1129 c         1198 g         1464 t
ORIGIN      
        1 gtcgggctgc ctgcagaact tgctctagtt cttgtgctcg gcaaaggata caaaatgggg
       61 ccgtcgaaac ttgagaatac catggacttc agaatctaaa gctcttttgc tggagattga
      121 gatcgattgc aattgtttga aaagctttct aagaaagaga ttctgaattg agttcagatc
      181 acgggattag gagctgaata tagcgatgag gtatgggtat agaaagttcg cgaaatagcc
      241 aatttggccg tgaagtggaa ctggccacaa atatctctca ttaataatga ctgagttcta
      301 atcttcacca aaggaactaa tagccgtgcg tgcgccacta tccacctcgg ttgcaagccc
      361 accttggaag actggcgaag tcaaaccgta attgcataac tgcttttttg tgggggtgag
      421 tcgtgaaact gaagagagtc attaccatct gcttgctact attcgtcttg atcagtattg
      481 cttattttgc gtacgatgaa atcaaactca tttctcagtc ttcgaagtct cgagaatcca
      541 ttcttgaaga acaggatgat ccttttcttc cagaagattt tgttgttctt tattactttc
      601 acggaactat ccgttgtaag gtatgcctgg acatggaggc aaacgcaaag aaagcagtcg
      661 agacacactt ttcttcacaa tatggaacgg ggaccttgag gtgggaagtg gtaaacatag
      721 accttccgga gaattaccac tatctagaag attacactat catgtataac actcttgtca
      781 tccagcaata catagatggg aagcccggtg aatggaagga actgtggcat gcctgggacc
      841 tttcagaaga ggaagaccag tacattgaat atgtgagaga tgaagtagct tcttttttgg
      901 gggtgaattg atggacaact ttgcgttttc aatgatttcc gctctttggt tcgggatttt
      961 gacctccatt agcccctgcc ctctagctac gaacattgca gcggtttctt ttatcagccg
     1021 gaatatcggt agcccaagaa agtcactttt agcaggtctt ctctacacac tgggaagggt
     1081 agtaacatat gttgccattg gcgcaatact tgtttcaagc ttgacggcgg ctccaggtct
     1141 gtcgagattc ctccagaaat acatgggtaa gttaattggc ccaattatga tcgttgtagg
     1201 catgtttctt cttgagttgc tgagtttcag aaacagcgga agaaatctgg atacaacatc
     1261 tcaagagaag atcaagagca aaggtctgct tggggccctg attcttggct ctttgttcgc
     1321 ccttactttt tgccctgtat cggctgcact attctttgga agcgtaatac ccctcgcagt
     1381 caatgaacag tccaggctaa tgattcctgc aatctatggt atcggcacag gcctacctgt
     1441 tgttgttgtt gccatcggaa ttgccataag cacgaagctg gtcgcgcaga tgttcaggcg
     1501 aatcagcaga ttcgaattct gggcgaggac aattaccgga gcagttttca tagtcctggg
     1561 aatctatctg agtataagga ggattttctt gtgaaacgct cagtattaca ccgcttcaca
     1621 aacagaggtc aagcaattat tcaattcaag actgctataa tctcactcca aactttgaaa
     1681 ggatgagcga aaagaaggca tagcctgcca tcatgactcc actggacaaa gcaagtgcta
     1741 cgtagaaatt gatgcccctt ctgagaagca atggcatagc aacaaagaaa atcagagaag
     1801 gaagaacaaa aaggagaatg ccggtcgaga gttctgcaac tttacttgaa ctgtttgtgt
     1861 cgttgtagag ccagaatagc gccagcatgg aagtaagagg taaagatgct ataagtcctc
     1921 caatgtatcc agacttcttg ctaacttcag aaacaagaac gattattacc gatgacacaa
     1981 ggattttcac gatgtatctc agcaaactcg ttcacctccg ctttgactct ttttgtccac
     2041 actgccaatt gatttttatg atctcgtatc actcctgaga ttctaataga ttaccatacg
     2101 ccagtttagc tcagcttgta atgaatcggt ccttcgtaaa aacaattacg attagtcctc
     2161 cagtggcaat gagtgaagcc atcataatga acatgccaaa ataagagtat tctgcaaccg
     2221 ttccggccag taatggcccc accatgaaac caatggctct cgccgaccta agaagtccta
     2281 gaaactcggc tcttctctcc ggcggtgaac tgagagatat catagtaccg gcaccgttta
     2341 tgaaggctcc aaaggcaata cctaggcaga agtacccaat tgcaatgaga acccagctct
     2401 ttgccacggc aaagaaaacc attgtgaata tcgttagacc tatgccgatc agtgtgctct
     2461 ttcttggtcc ggttttatat atcacccttc cgaagaagat atgagaaaag atctgcataa
     2521 aggggtttat cgaagtgata attgccgtgg ccgctcctga caagccgatt ctttcagtca
     2581 tatatattgc aattatcgaa gtggctccag ccgttccgag ctgtctcaag aaagtgccgg
     2641 cataaactgc ccacagtcca ttttcccaga gaggcgtaga atcttctttc aagggaaaca
     2701 acctgttcaa gaagcccttt ctacgagaga cgaggtgttc ctcttcattc aaaaaaagta
     2761 caggtatgac agcaattagg gcaacgaagg cataccagtt aatagcctga ctcacagtga
     2821 aaaagatcag aagaatcgag agcataattc tgccagttag cattccagtt gaatttgcag
     2881 tattcaatat cgacagttct cttgaagtat ttctgaccga atcccgtgaa tgttctgtgc
     2941 tcagagccat tgctatgggt tcgaacccgg cggtgaagaa agcaatcaat gaagaaaaga
     3001 gaatgaatac tatgggaatc tcaattactg aataagcaaa gtagagaagg gccggcgtga
     3061 gtacgagtaa tatcatcagg agttttcgtt tcttaagtcc gtcagatatt gctccccagg
     3121 ccggagaacc gaaagtctcc acagctcctc ttactgttgt aagaagccca accataagaa
     3181 gtgaggcccc aagctctctt agcttcagct gtaagagcaa gccaaaaccg aacatcacaa
     3241 agaacactaa cagagatgtc agcaaaagtg gaagacgttt gttatgcaag ggagattctc
     3301 ctctagtttg attgacttat caattatagc atcagcttta aatccctcaa attaggcaaa
     3361 atcagcgtgt tacggatcca aattcgtatg taaaccatca tctcttatcg aagaatcctg
     3421 ttctcttcct ggtttcacag cttcttctca agaagaactg agaattcttt cataccgatg
     3481 gcttcagacc aaagatgata atcgctttca aatgcacatt caaagaatga ctcttcaacc
     3541 ccgcttattg ataggtactc atttaggaaa gaaagtaagt ttcttcctat gcccattcga
     3601 cgaaaacgtt tgctgacaaa gacaaactct atagatccaa gaagactgta agtcataata
     3661 tcttcgaaga cagaatagat tagaactccc accgtttctt catcttgccg gcaaattaga
     3721 attctacctc ctgagtccat gatttcattc agattgaaga atagttcact tgacgtcatt
     3781 tgaagctgat tttctacgag cgagggggaa gaggagattt cgacaaagac ttttccaaga
     3841 tcttcaggaa gaccgcgaaa gatttcgaag gcatttgtcc tagagtctgc gagtcgttta
     3901 ttggtactca aggtaattct tttttcgcta aaaccctcac ttttcagcat atgccatcca
     3961 gtcccgaatc tataagtatt gccggtaatc ttctgaaaaa aaggcttgag ttcctttaaa
     4021 gcagacctaa gaagagtcct tcctgctcct tctcctctgt gctctttctt gacatataga
     4081 tcatagatgt gtccagataa actggaagaa aaccgttcgt ccctctgtgg cccgaagtgg
     4141 acatgcccaa ttatctctcc ttggcctctc aaaacaagca atttatcgcc tctcttaaga
     4201 aaatcaaagt agacttcctc aatcggcctg agttctgaaa gcgaatacct accattgctc
     4261 gattcgggaa tcgagtgata catcttctcc ataatccctt ttgactcctc agaagagggc
     4321 ttatcgacaa agaaatcttc aatcataata ccgcctatag gggagataag tgataccgtt
     4381 ttcatccaac tcctgtatga ttatagtaac tttgcacatg gaggtgtagc agtgaaaaga
     4441 atctctctat ttttggtttt gctggttttc tctgtgattg ctcttggaca atcggagttt
     4501 atgattccta tgagcaccga ggatttcgga ctgaggggac ctgttaagtc agcaaaaata
     4561 ctatacccgg agaacgaaat ggatctggga gtcgatgagg agttcttcct ctctggatac
     4621 agtgttcttt cgttcaacga gaatggcagt cttgtttcgc agattgaata tgacaaagac
     4681 ggtaatgaac agagtgcgat actcttcaat tacgacgcca atggaaaact actttctgtg
     4741 tctggaagag agaatggtgt tgaggaaaac gtcgacgaaa taaaggtcga aaacggaaga
     4801 atagccggaa taatcgttga agactctgaa ggtgactcca atctggttat ggaatacgat
     4861 gaagcgggaa gactagtcgc tcagaagatc agtggaatga gcgaggggca ggaaatcgag
     4921 atgaccatat cgatgaaata cgatgacaac ggaaatctgg ttgaggaatc ctttggaatg
     4981 atgggaatgg tccttacaaa gaccattttc gagtacaatg ataagaacca gagcgttaga
     5041 gagctggagt atatgtacat gttcgctgaa ccaggcacag aacccgaacc aatagtatct
     5101 actcttgagt acaacgaaat gggtgacgtt gttcttagag tatctgacag tagttttggt
     5161 gaagagaaag aagccgttgt ttatgaatat gaatacgatt ccgcgggcaa ctatacacat
     5221 aaggttgctt actatgttat ggatatcgcg gagctccaaa acgaaacctg gaaagagacg
     5281 gcaatgattg aagaagaaga gacacgggaa attgagtatt actgattgaa tcaataagaa
     5341 aaagagggtg gaaacaccct ctttttcatt ttgaaatatc tgctttctct taaaag
//
LOCUS       DAOQYI010000087         5225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_16127_length_5225_cov_50.173308, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000087 DAOQYI010000000
VERSION     DAOQYI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16127_length_5225_cov_50.173308"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..945
                     /gene="sufC"
                     /locus_tag="PKL93_10735"
     CDS             193..945
                     /gene="sufC"
                     /locus_tag="PKL93_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNQ71774.1"
                     /translation="MTLLEAKNLHVVLAEDDVKILKGLSLTIGNGELHVIMGPNGSGK
                     STLANVLMGNPKYRVTGGTVKFMNKNISRLTVDERARMGMFMTFQHPQEIPGIRLRSF
                     LMSVSQSTGSRVPLLKMSKEIDELSLELGLESELIDRYLNVGFSGGEKKKAEIVQMNF
                     MKPRFSILDEIDSGLDVDAMKSIALSINRFRNPENSVLLITHYQRLLEYVEPDRVHIL
                     IDGLIAMSGGKELVREVEENGYEAIFDSLKGV"
     gene            948..2342
                     /gene="sufB"
                     /locus_tag="PKL93_10740"
     CDS             948..2342
                     /gene="sufB"
                     /locus_tag="PKL93_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNQ71775.1"
                     /translation="MREKVFLDSSRFDFLSNTKYRFMSERGFGESQVIDISKRKNEPE
                     WMLKKRLQSFKAFSLSSKPGFGVSTESLDLSRIISYLGPDSDKHRSWDDVPQEIRETF
                     EKLGIPEAERESLAGVGAQFDSEIVYQNIRKELEELGIVFLDMETAVKKYPELVRRFF
                     MKAVPPTDHRFAALHGAVWSGGSFVYVPKGVKVPLPLQAYFRMNVESSGQFEHTLIIA
                     DEGSDLQFIEGCSAPRFNDLNLHVGMVEIFVLKDAKVRYSTIQNWSKNTYNLNTKRAI
                     VEEGGTMEWISGSLGSMKTMLYPSSVLRGRGARAEHLGITYAGPGQHMDTGSKVIHLA
                     PDTSSLVDARSISVGGGWAFYRGFLDISRSAMNSRSSVKCTALMIDNESRADTVPIIQ
                     VMNNAAEVGHEARIGRLGEEEIFYLMSRGLSESEAKAIIVRGFMEPVSHQLPVEYAVE
                     LNRLIDTEMESSIG"
     gene            2353..3555
                     /locus_tag="PKL93_10745"
     CDS             2353..3555
                     /locus_tag="PKL93_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNQ71776.1"
                     /translation="MEMTTELVHKNFEMVKPTRREELSIKDYDNRDFLISAEASSPAM
                     LAFMSNRYSEYRTLAFPSWKRLKLTGMKLPEISPRRSLSYSDPFVRPLISMKSDEVEL
                     LDKLDFEGLDRKILLLGDIFFSEGSMISIPPNKSAEKSYSVAFNRDSRISSNMFVLGE
                     NSSLKVVIDSGRFGNWLLQNNRFLIKEGSSLELLVLNRVTERGHVFINNLYLLEKNAK
                     LRVFEVNCGNASTAAFHLALLEGERALASFMPLFVARGDTVLDMQYVVRHRANWSEGR
                     ISGSGVLLDNSRSVFRGTIDIKRGAKGVKGSEYSNILMLSESARADTIPSLLVGENQV
                     DAFHAATVGSIDAQKLFYLMSRGLTRDQSMSIIVQGKFEPTLREIDEIFPDHSGVVRD
                     EFERDIRY"
     gene            3527..4753
                     /locus_tag="PKL93_10750"
     CDS             3527..4753
                     /locus_tag="PKL93_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ71777.1"
                     /translation="MSLKETSDISIKEVRRDFPVFRHYPSLAYLDSAATAQKPQSVID
                     AVKGFYERENANVHRAVYPLAEKSTALYESSRSEVARFIGAERDELIFTKGSTESLNI
                     IANALATSGRYKTFVVPTFEHHSNFLPWQYHSLKNEIKFVALPIMGAELTMTEVEENL
                     TGVEAPFVFSIGGLINATGYRTPFEDLTELVHSFGGLIVIDGAQLVPHEIFDFKKVNS
                     DFLVFSGHKMLAETGIGCLVGRAELLDDLTPFVFGGGMVDRVSIETSSYAKERTSRLE
                     GGTQNISGAVSLASACRYLNKIGMSAIHKHVSYLTETAREMLKQISNVTVYSPPDSHA
                     ILLFSHKNIHSHDLAEFLGRTQEVAIRSGHHCAQLQMEALGITSACRASFYFYNTVED
                     VERLIEGISKAERWFL"
     gene            4750..5190
                     /locus_tag="PKL93_10755"
     CDS             4750..5190
                     /locus_tag="PKL93_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNQ71778.1"
                     /translation="MNFEQLYSDFIMYHFKNNSHRGRIEDCTYTVEGSNTSCGDSITL
                     YLIIGRGVVDTIGFEGEGCAISQASAAVMTEILEGKSLDEAAATIVNFEKMIRGEDYD
                     GSKIGDAAFFEGLKEHPMRAKCVILPWKTAAKVLVSKQQKKTGA"
BASE COUNT         1531 a          993 c         1320 g         1381 t
ORIGIN      
        1 ctggaataag attggcaaga cccatgagga cacctataaa gactacatag agcatcaagc
       61 acctccatac cttaatttct ccaattatat atccttgaaa cggtatcgta cttattggtg
      121 ttgattatcg gcaataattg tcggcattat gttataatcc agataataat taccgggttt
      181 ggagggaatg gaatgactct tttagaagct aagaacttgc acgtagtatt ggcggaagat
      241 gacgtaaaga tattgaaagg gttgagtctc acaataggaa acggggagct acacgttatt
      301 atgggaccca atggtagcgg taaatcaacg cttgcgaatg tgctgatggg gaaccccaaa
      361 tacagagtta ctggtggtac tgtgaaattc atgaataaga atatatctag attaaccgta
      421 gatgaaagag ctcgaatggg aatgttcatg acctttcagc atcctcagga aattccaggg
      481 atcagactca ggagctttct tatgtcagtc tctcaaagta cgggcagcag ggtgccattg
      541 ctgaaaatga gcaaggaaat cgacgaacta tctcttgaac ttggtctaga aagtgaatta
      601 atagacagat atttgaacgt aggtttttcc ggaggagaaa agaaaaaagc cgagatagtt
      661 cagatgaact tcatgaagcc aaggttttcg attcttgacg aaatagattc cgggttggat
      721 gttgacgcta tgaaaagcat agcgttgtca ataaacaggt tcagaaatcc agaaaactca
      781 gttttactaa tcacacacta tcagagactt cttgagtatg ttgaacccga ccgtgtccac
      841 atcttgatcg atggattgat agcaatgagt ggaggaaaag aacttgtcag agaagtcgaa
      901 gagaatggat atgaagcgat ctttgactca ctcaaggggg tctgatcatg cgagaaaagg
      961 tcttcctaga cagttcaagg tttgattttc tttcaaatac aaagtaccgt tttatgagcg
     1021 aaaggggatt cggagagagc caagtaatcg atatatcgaa aaggaagaat gagcccgaat
     1081 ggatgcttaa gaagcggctt cagtctttca aggctttcag tctctcgagc aaaccaggat
     1141 tcggagtaag cactgagagt cttgatctga gcagaataat ctcctatctc ggtccggatt
     1201 ccgacaagca tagatcctgg gacgatgttc cacaagaaat tagggagacc tttgagaagc
     1261 ttggcattcc cgaggcggag agagaatctc ttgctggagt cggtgctcag tttgactcgg
     1321 aaattgtgta tcagaacata aggaaggagc tcgaagagct tggtattgtc ttcctcgaca
     1381 tggagacggc tgtcaaaaag tatcctgaac tggtaaggag atttttcatg aaggctgttc
     1441 ctccgacaga tcacagattt gcggcactgc atggagctgt ctggagtggc ggttcattcg
     1501 tttatgtacc caaaggagtc aaagtcccac ttcctcttca ggcatatttt agaatgaacg
     1561 tggaaagcag tggccagttt gagcacacgt tgatcattgc agatgaaggt tccgatctcc
     1621 agttcataga aggctgttca gctccgagat tcaacgatct caatcttcat gttggcatgg
     1681 tagaaatctt tgttctaaag gatgctaagg tcaggtactc tacgattcag aactggtcca
     1741 agaacacgta caacttgaat accaagaggg caattgtcga agaaggcgga acgatggaat
     1801 ggatatctgg ttcactggga agcatgaaga ccatgctcta tccatcatct gttctaagag
     1861 gcaggggtgc cagagctgag catcttggaa ttacctatgc cggtcccggt cagcatatgg
     1921 atacaggatc gaaggtgatt cacctggctc cagacacaag ttcactggtt gatgccagaa
     1981 gcatcagcgt gggtggaggc tgggcctttt atcgaggttt tttggacatt tctcgaagtg
     2041 caatgaactc aaggtcgtcg gttaagtgta ctgctctaat gatcgataat gaatcaaggg
     2101 ccgatactgt tccaataatc caagtgatga ataatgctgc agaagtcggt catgaggcca
     2161 gaattggaag attaggagaa gaagagattt tctaccttat gagtcgtggc ttaagtgaat
     2221 cggaagccaa agcgatcata gtaagaggtt tcatggaacc tgtttcacat cagctacctg
     2281 ttgaatatgc agttgagctg aacagactga tagacacgga aatggaaagc agcataggat
     2341 gaggtggaaa caatggaaat gactacggaa ttggttcaca aaaacttcga aatggttaaa
     2401 ccaacaagaa gagaggaact gtcaattaag gactacgaca accgagattt tctgatttcg
     2461 gctgaggcat cttcgcctgc gatgttggct ttcatgagta acaggtattc agagtataga
     2521 acgctggcct tcccctcatg gaaaagattg aagcttaccg ggatgaaatt gccggaaatc
     2581 tctccaaggc gtagtttgtc ttactcagat ccttttgtca gaccgcttat ttcgatgaag
     2641 agcgacgagg tagagcttct cgataaactg gattttgaag gtcttgacag aaagatcctt
     2701 ctgctaggag atatcttctt cagcgaagga tcaatgattt ctatacctcc gaacaaatca
     2761 gcggaaaagt cttactcagt tgcatttaac cgtgattcaa ggatttcgag caatatgttt
     2821 gtcttaggag aaaattcgag tcttaaagta gtaatcgata gcggacgttt tggaaactgg
     2881 ctgcttcaga acaacaggtt cttgataaag gaaggctcat ctttagagct tttagtccta
     2941 aatagagtga ctgaaagggg acatgttttc atcaataacc tgtatcttct tgagaaaaac
     3001 gctaagctta gggtatttga ggtgaattgt ggcaacgcgt caactgcggc ttttcacttg
     3061 gctcttttag aaggagagag ggctcttgca tcttttatgc ctctttttgt ggcgagaggt
     3121 gatactgttc ttgatatgca atatgttgtt agacacagag caaattggtc agagggaaga
     3181 atttcagggt cgggtgtact acttgacaac agcaggagtg ttttcagagg gacaatagac
     3241 attaagagag gagcaaaagg agttaaggga agcgaataca gcaacatctt aatgctatcc
     3301 gagtctgcaa gggctgatac aataccttca cttcttgtgg gagagaatca agtcgacgct
     3361 tttcatgcag caactgtggg atccattgac gcgcagaagc tcttctatct tatgtcgaga
     3421 ggacttacaa gggaccagtc tatgagcatc atcgttcaag gtaagtttga gccgacttta
     3481 agagaaatcg atgagatctt cccagaccac tcgggggtgg taagagatga gtttgaaaga
     3541 gacatccgat attagcatca aggaagtcag aagggatttc cctgtctttc gacactatcc
     3601 gtcactagcc tatttggata gcgcggcgac cgctcaaaag ccgcaaagtg taattgatgc
     3661 ggtcaagggg ttttacgagc gtgaaaatgc aaatgtgcac agagctgtct acccacttgc
     3721 ggagaaatcg acagctcttt atgaatcaag tcgaagcgaa gttgccaggt tcatcggagc
     3781 agaaagagac gaattaatat tcacaaaggg ctccacagag agtctgaaca tcattgcaaa
     3841 tgcactggct acttccggtc ggtacaaaac ttttgtagtc ccaacgttcg agcatcacag
     3901 taacttcctt ccgtggcagt atcacagcct gaagaatgaa attaagttcg tggctttgcc
     3961 catcatgggg gcggagttga ctatgacaga ggttgaagag aacctaactg gggtagaggc
     4021 gccgtttgtc ttttctattg ggggactgat taatgccaca ggctacagaa ctccttttga
     4081 agatttgact gagcttgtcc actcttttgg cggtttgata gtaatcgacg gagctcaact
     4141 ggttccgcat gagatctttg atttcaagaa ggtcaactcg gactttctgg tcttctccgg
     4201 tcacaaaatg cttgcagaaa ctggaattgg ctgtcttgtt ggtagggcag aactcctgga
     4261 tgatctcaca ccatttgtat tcggaggcgg tatggtagat cgggtaagta ttgagacttc
     4321 gagctacgca aaggagagaa cttccagact ggaaggcgga acacagaaca tttccggtgc
     4381 cgtttcactt gcatcggcct gcagatacct gaacaagatc ggtatgtccg cgatacataa
     4441 gcacgtttca tatcttacag aaaccgctag agagatgcta aaacagatct ctaatgttac
     4501 tgtttacagt ccgccagata gtcatgcgat cttgctcttt tctcacaaga atatacactc
     4561 tcacgatctg gccgaattcc ttggaagaac tcaggaagtt gcgatcagga gcggtcatca
     4621 ctgtgcccaa cttcagatgg aggctcttgg cattacttct gcttgtcgag catcattcta
     4681 cttttacaat acggttgaag atgtggaaag acttatcgaa gggattagta aggcagagag
     4741 gtggttctta tgaacttcga gcaattatat tccgacttca taatgtatca cttcaagaac
     4801 aatagccacc gaggacggat agaggattgc acttacacag tcgaaggttc gaacacttct
     4861 tgtggagatt cgatcactct ttatctgatt attggaagag gagttgtaga tactattgga
     4921 tttgagggtg aaggctgtgc gataagtcag gcgtcagctg cagtcatgac agagattctt
     4981 gaaggtaagt cacttgatga agcagctgcg acaattgtga attttgagaa aatgatacgt
     5041 ggtgaggatt atgatgggtc aaaaataggt gatgccgctt tttttgaggg gttgaaggaa
     5101 catcctatga gagcgaagtg cgtaatcctt ccctggaaaa cagctgcaaa ggttttggtt
     5161 tccaaacaac aaaaaaagac cggtgcataa caccggcaat actttgcgct gaagtaaccc
     5221 tacga
//
LOCUS       DAOQYI010000088         5102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_16619_length_5102_cov_71.437686, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000088 DAOQYI010000000
VERSION     DAOQYI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16619_length_5102_cov_71.437686"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PKL93_10760"
     CDS             complement(<1..1074)
                     /locus_tag="PKL93_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNQ71779.1"
                     /translation="MDFPGSVQNFIYLLSKLGQQNKMLSEKAIEYFWSSLLLKKLSQN
                     VQFSDVFWRFVFFGKSGKERADLMESAISELIVEHPEFAFMDFKGELGGAFKEDSPLF
                     YRTCAYADGMIDGLSIRDIDLLIGPFYTGMFSFAIDSNLLYSDMIVPMEILDMMVDLL
                     EIEPGMTVYDPAFGYGRAFYTIFRREGFDYTSFAGLEIDPRKWRLTKIILSLVGIDVS
                     KLVRGDSLVKPTKETLYVEEQYDRVIIEPPTGERMVLQELKTDFFLRNILNISSGLIS
                     QWDFINHGMRSMKDDGKLVTLITRGELREGNKEIIDNDWLEAVIQLPLGAVHGGNDDC
                     CLLVLNKAKPIGRKGQVFMSVSKS"
     gene            complement(1074..>1751)
                     /locus_tag="PKL93_10765"
     CDS             complement(1074..>1751)
                     /locus_tag="PKL93_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ71780.1"
                     /translation="LTYDFYIGKYTVTCQEYSRFCRETGAEEPVDEESPNGTKPVTNV
                     KWLDAISYCNWLSEKEGLPPAYDELGKLLDCDGSHTTDLRRVAGYRLPTEAEWEYAAR
                     GGNKSRDYIFAGSNDVDEVAWYIENSSDRTREVGLKSPNELGLYDMSGNVWELCSDNS
                     DRYSEGLEINPYGETGVFKILRGGSVREDREEVTISARDFTGERYTNSSIGFRIAKTV
                     LVDGGEQ"
     assembly_gap    1752..1851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1852..2247)
                     /locus_tag="PKL93_10770"
     CDS             complement(<1852..2247)
                     /locus_tag="PKL93_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71781.1"
                     /translation="MMTELPSMEMPEWIRNPGEFDIRNILQDSLCYLASGLHGHFVKC
                     YMGNVYSFVYIDYFISREDLLQDMAEYGFTGYRVIRQESIDPYRLHRGGWDALTWYYR
                     EDDPRGERRFDRFRGDRERGYFAGYRDILN"
     gene            complement(2909..4708)
                     /locus_tag="PKL93_10775"
     CDS             complement(2909..4708)
                     /locus_tag="PKL93_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNQ71782.1"
                     /translation="MDLVSFAENFIYLLFKLREEDRSLSERAIEYFWTNMLLRKNSFR
                     ENYFDVFEEFALFGKSGIERAALIEKALKEIISRYPRFRFIDYKGKYVWIFSEGPELL
                     QKTCAYVDSFVDQLAMKDIFDKSGSLYEALLSVSMESDLLEGDSAAPPQVIDSMVELL
                     DVRHGMSIYDPSTGFGRTLYGIFKNNTLENLEYAGLEADPYRHRIGKIALILLGLNAV
                     NLKQGDPLTRPEDYSLLSNSQYDRVILEPPSSEKLLSYIIKTGYLTRGILEVSKVPGS
                     QWSYIKHGIECLKPTGKMVSLIETRALENPICEIVDNDWLEAVIELPVGKGNFLRRET
                     SILVINKSKPTQRKGKVLLVVPESEKQIDWFDIQNPIDWPSRFTEQYRGWLSEPNRSG
                     IVSIQTITANGYTLRPRDYAISAGMPRRKKVSFEELINAPMIELKEIGEFIIGRNPDR
                     VGYGPEHGLNSETPLHGKKESGRIYILERVKTDSEGVIDLYRSASQLRTPLEVTEPGT
                     LRKSDIVISVWSGEESVGIFGDILDDETVILSRDHVVLRVREEYDALEVLRVLRSDYG
                     KHLIHKAKADMFFGGMSFDALEEMRIPAIFGRK"
BASE COUNT         1150 a         1315 c         1127 g         1410 t
ORIGIN      
        1 gctcttggat accgacatga agacctgtcc tttcctgccg atgggcttcg ccttgttaag
       61 gacaagcaaa cagcagtcgt cgtttccgcc gtgaacggct ccaaggggga gttgtattac
      121 tgcttcaagc cagtcgttgt ctattatttc cttatttcct tctctcagct ctcctctcgt
      181 aatcagcgtc acgagcttgc cgtcatcctt catcgagcgc attccatggt taatgaagtc
      241 ccattggctt atgagaccgg acgagatatt tagaatgttt cggaggaaga aatcagtctt
      301 cagctcctgc aataccatgc gctcgcctgt tggtggctcg ataatcaccc tgtcgtactg
      361 ttcttccaca tagagagtct ctttcgtcgg cttcacgagg gaatcgcccc ttacgagctt
      421 cgagacgtct atcccgacaa gggaaaggat aatctttgtg agtcgccatt tcctagggtc
      481 gatttcaagt ccggcgaagg aagtgtagtc gaagccttct ctcctgaaga ttgtgtagaa
      541 ggcccgtccg taaccgaaag cggggtcgta aacggtcatt cccggttcga tttcgagcag
      601 atcgaccatc atatccagta tttccatcgg cactatcatg tcagaataga gaagattgga
      661 gtcgatcgca aatgagaaca ttcccgtgta gaaggggcct atcaacagat ctatgtccct
      721 gatggagagt ccgtcgatca tgccgtcggc gtaagcgcag gttctgtaga agaggggcga
      781 gtcttctttg aacgccccgc ccaattctcc tttgaagtcc atgaaagcga attctggatg
      841 ctcgactatg agctcggata tcgcgctctc catgagatcg gccctctctt tcccgctctt
      901 tccgaagaac acgaacctcc agaagacgtc actgaattgt acgttctgtg atagtttctt
      961 caacaaaagg gagctccaga agtattcgat tgccttttcc gagagcatct tgttttgttg
     1021 accgagtttg gaaagaagat agatgaagtt ctgcacgctg cccggaaagt ccatcactgc
     1081 tctcccccgt cgaccagcac tgtcttcgct atgcggaagc ctatagatga gtttgtgtaa
     1141 cgctcccctg tgaaatcacg agccgaaatc gttacttctt cacggtcttc acgtacgctt
     1201 ccgccacgaa ggattttgaa cacaccggtt tccccatacg ggtttatttc cagtccctcc
     1261 gagtatctgt cgctgttgtc gctgcacagt tcccagacgt tgccggacat atcgtaaagc
     1321 ccgagttcgt tcgggctctt caacccgacc tctcgagttc tgtcggatga gttttcaata
     1381 taccaggcga cctcatcgac gtcgttactt ccggcgaaga tataatctct gctcttgttc
     1441 cctcccctgg ccgcgtattc ccattcggcc tcggtcggca acctgtagcc ggcaactctt
     1501 cttaagtcgg tcgtgtgtga gccgtcgcaa tcaagcagct ttcccaattc atcgtacgcc
     1561 gggggcaggc cttccttttc gctcaaccag ttgcagtagg agatggcgtc taaccatttc
     1621 acattcgtaa ccggttttgt gccgttcggg gattcttcat ccacgggttc ttctgcgccc
     1681 gtttccctgc agaacctcga gtactcctga cacgtgacgg tgtatttgcc gatatagaaa
     1741 tcataggtaa gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattcagaat
     1861 gtctcgatat cctgcgaagt aacctctttc gcggtctcca cggaagcgat cgaaccgcct
     1921 ctctcctcgc ggatcgtcct cgcggtaata ccacgtaagc gcgtcccatc caccccggtg
     1981 gagtctgtac gggtctatcg attcttgtcg gatcactcgg taacctgtga acccgtactc
     2041 ggccatatcc tgcagcagat cttctcggct tatgaagtag tcgatgtaca cgaagctgta
     2101 aacgtttccc atatagcatt tgacaaaatg gccgtgaagg ccgctggcaa ggtagcagag
     2161 agagtcctgc agaatgttcc ttatgtcgaa ctcccctggg tttctgatcc attccggcat
     2221 ttccattgac ggtagttccg tcatcaaact gaccactcct ccttatgatt tttcgcgatc
     2281 gaaatcgcta cccgttaaac gggtctattt tcttctctga cctcactaca accgcacaat
     2341 gggattgtca ctggatcgga aacgggagaa agtcttatga gttgatattc tgtggagctg
     2401 agtgatattg ctgttaggac catgcgatgc ttaaaaaagc tacaagaaag acagcaccgg
     2461 ctggatacaa cggcgaacag aaatgtccgt gtgggatttg gattctgaaa gtcgacttaa
     2521 ggatactatg gtgtccgtgg tttctggatt cgacacaagc aattgattcg atagggtttg
     2581 atctaccttg gagaccttaa ttaaacggtt tttgagtagc ggagttaatt cacatcctgt
     2641 tgattcgaat tgtctagaat aactaagtta attatacagt aaagttcatg ccagtaagtt
     2701 acggtgttat atctataata atcatctatt cattacgaaa cacttacata attgagggat
     2761 gaatccgctg actcgtctat ctcgcacgaa agggacggcc ctcatagatc tcaaacagtc
     2821 tcgcaatgct agaggtgtca agtccggggc cgatcgattc ttccccaagt ttggcgggcg
     2881 tgtttacgga agagtctttt tcaattcgtt acttcctccc aaaaatagcc ggtatcctca
     2941 tctcttccaa tgcgtcaaac gacatgccac caaagaacat gtctgccttc gccttatgga
     3001 tcaaatgttt tccgtagtca ctacgaagga ctctcaagac ttcaagagca tcatactctt
     3061 cccttactct cagtactacg tgatctctcg aaagaatcac cgtctcatcg tcaaggatat
     3121 ctccgaaaat acctacgctc tcttctcccg accagacaga gataacgatg tcagactttc
     3181 tcagggtccc tggttctgtc acctccaacg gtgtcctgag ttgactcgcc gagcgatata
     3241 aatctatgac gccttccgaa tcggtcttca ctctctccag aatatagatt ctgcccgatt
     3301 ccttcttgcc atgaagcggt gtctccgagt tcaggccgtg ctcagggccg taaccgactc
     3361 tatctggatt cctgccaata atgaactccc caatctcttt aagctctatc attggggcgt
     3421 tgatgagttc ttcaaaactc actttctttc ttctgggcat acccgcagat atagcatagt
     3481 ctcgcggtct cagggtgtac ccgtttgcag ttatcgtctg aatcgagaca atgccgcttc
     3541 gattcggttc cgagagccat cccctgtact gctctgtgaa tctcgatggc caatcgatcg
     3601 ggttctgtat gtcgaaccag tcgatctgct tttccgattc gggaaccaca aggagcactt
     3661 ttcccttcct ttgagtgggt ttggatttgt ttattacgag aatgcttgtc tctcttctta
     3721 ggaaattgcc cttgccgaca ggaagctcta ttacggcttc cagccagtcg ttgtcgacta
     3781 tctcacagat cgggttttca agtgctcttg tctcgataag gcttaccatt ttccccgttg
     3841 gcttaagaca ttcgattccg tgtttgatgt agctccactg gcttccagga accttcgata
     3901 cctccagaat gcctcttgtc agatacccgg tcttgattat gtaagagaga agcttctcgc
     3961 tggatggcgg ctcgagtatc accctgtcgt actgagaatt tgacaggagc gaatagtctt
     4021 ccggcctggt tagcgggtct ccctgcttca gattcacggc gttcaatccg agtagaatca
     4081 aagctatctt tccgatcctg tgtctgtatg ggtctgcctc caggccggcg tattcgaggt
     4141 tttcgagggt attgttcttg aagatgccgt aaagggtcct tccgaagccc gttgatggat
     4201 cgtatatgct cattccatgc cggacatcca gcagttcgac cattgaatcg atcacctgcg
     4261 ggggcgccgc cgaatctcct tcaagcaaat cggattccat ggataccgag agcagagcct
     4321 cgtacagaga gccgctcttg tcgaagatat ccttcatcgc caattgatcg acgaacgagt
     4381 cgacgtaagc gcatgtcttt tgaagtagct cgggaccttc gctgaagatc cacacatact
     4441 tgcctttata atcgatgaat ctgaaccggg gatacctgct gattatctcc ttcaacgcct
     4501 tctctatcag cgctgccctt tcaattccac tcttgccgaa aagagcgaat tcttcgaaga
     4561 catcgaagta gttctctcga aacgagttct ttctcagcaa catgttcgtc cagaagtact
     4621 ctatggccct ctccgacagg cttcggtctt cctctcgcaa tttgaacagg agatagataa
     4681 agttctctgc aaaacttact agatccattc caagccccct ttttcatttt tagtttaatc
     4741 atgttgtctt gctctgactg ttttaacctg cgttccatgg aatctccctt aacatgtttc
     4801 cccgttcttc acctcctgaa atctcactgt tcgtatcttc gcagacttag ccataggcga
     4861 cacaccggag cggcctattg ctcgcgcttg accgatagat cgatgaaaaa agcattttca
     4921 ctatatgtga atagagagtt tcgccgagaa tcaatctcgg aaccttacgg aggataacct
     4981 tctctgatga ctctggaaat gcaatacgat tcaactaccg caagccttcc ttcgcacaag
     5041 aatatattcg ggtgagtgac gacaatgaat aaagttgctg gacgtaacgg caagactatt
     5101 aa
//
LOCUS       DAOQYI010000089         4877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_17644_length_4877_cov_58.524886, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000089 DAOQYI010000000
VERSION     DAOQYI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17644_length_4877_cov_58.524886"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..241
                     /locus_tag="PKL93_10780"
     CDS             2..241
                     /locus_tag="PKL93_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71783.1"
                     /translation="MIGGMTLAVGYGTKAMQRVQDPTTCENFVDADGDGINDNCPNDG
                     VRELKNAQDGTGYRHGASGEKGPGLGRGFGKAAQL"
     gene            1061..2425
                     /locus_tag="PKL93_10785"
     CDS             1061..2425
                     /locus_tag="PKL93_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ71784.1"
                     /translation="MDFSTVAFYNPWWDEKSKNTDLTFHLVDKYESSSFKRDYNGFFD
                     LTGNNLYVVRGPRKIGKSTLLKFTIAKLLKSGERRSVLYLPLDTISRVDELRSLLFQY
                     LQFTRNEKKRYVFIDEITMVDQWQRAVKEVRDNTEMVNDFFALTGSSAIDLKRSSERL
                     PGRKGQSGKDILLLPLTFREYLSCKKVEGLPKCQPEDILKMVPADAFELLIKDEILKT
                     EFFNYVASGGFPSAIEAYSKGESRETLINTFWEILIGNIERYGLSRAKLIQILTYVSS
                     RLSSRFSWSSAASETEIDTKTFQKYVEVLGIDYLLLTLKHMDQKTHLPSEKKQKKIYY
                     CDRLISDVVSQKLGVQLERSAILENIMTTNCAFAFGKNLDNGLDSITDFGYWYSKEGK
                     EIDLLVKDVPVELKYQNNITNQDLSTIKRHFKKGIILSKHTIDLSGDIKILPLHIFLA
                     ITGQ"
     gene            3395..3727
                     /locus_tag="PKL93_10790"
     CDS             3395..3727
                     /locus_tag="PKL93_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71785.1"
                     /translation="MKERRGIFELVDLNSWRELGVAAPGRNEKYWFVNHFGEEWLFKI
                     PKVGTTEHVSEKLAYEIAKLVGIEAAETEFAIYKGRLGTVSKNFVEVDKGENLIEMLD
                     LIQKMQPG"
     gene            3740..4285
                     /locus_tag="PKL93_10795"
     CDS             3740..4285
                     /locus_tag="PKL93_10795"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNQ71786.1"
                     /translation="MKDTLTGREYSLELVIDVIRATREALTTKVMQYLIFDALIGNSD
                     RHHGNWGIIYGGLGEIRMAPSYDHSASLGSKIREEDIENILKDKRRFMANVETKAKSL
                     LVFSGKRKVTHKELLIYIRDNFADSELVSTILRMASKINRTNVNDIIDLVPCEILSRT
                     TKDLLLELIIAKRQLVEEVFG"
     gene            4282..4626
                     /locus_tag="PKL93_10800"
     CDS             4282..4626
                     /locus_tag="PKL93_10800"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNQ71787.1"
                     /translation="MRKAVLYYRAKPDRKIPIGFLVFDGKHYSFEYDETALKNSETSS
                     LIDILPFSRQTVTYSNKLFPFFSRRLPDKKRRDYHTILDRFGIRNNAELELLFVNNGR
                     LPTDNFEITEIR"
BASE COUNT         1415 a         1072 c         1118 g         1272 t
ORIGIN      
        1 tctgatcggt ggaatgacat tggcagtagg ttacggaacg aaggcaatgc agagagttca
       61 ggatcctaca acctgtgaga actttgtgga tgcagacggt gacggaataa atgacaactg
      121 cccgaatgac ggtgtaagag aactgaagaa tgcccaggac ggaacaggct acaggcacgg
      181 agcctctggc gaaaagggac cgggactcgg cagaggattc ggaaaagcag cacagctgta
      241 aaattaaacc cctagtaatt caagtcaagg gcccgaaagg gtccttgttc ttataatgat
      301 gaaatcagaa taacagcctt ccaaacccac agactcctcg tttcttctgc tctgtcaccc
      361 cgaaacgagt tcgggatgac acagcgaggg acttccaaaa gccgctgtcg ggataatttc
      421 ctcagttgca ttgaaacctg aattgattgt cgctctcatc acgagaccca gtatcctgta
      481 ctcgataccc tgcccttcgc gtcatctgaa cttgattcag catcccgttc taagacccgc
      541 tgtacgctta agaacgaaga cccgcttttc gctgatcgct gaacgcttac aagagccgag
      601 aaagcgtatg ttcgagagtt cgcttcgctc acgagaagac gagagcgaaa tagggactga
      661 cgattctcct agcgtctgtc ccgtttttcg cgtgggccag ggatcctgac caggaccatt
      721 gtcaggatga cgtttggggg agtcatcccg taatgcctcc gctgctgtca tcccgtaaag
      781 tttgccctga atccactcac atagtcatcc cgtaatgctc ctatacggga tctcgttcct
      841 tttcttcctt ctctccctct ctcttttttc tccctctgtc tctctctttc ctaaaaaatg
      901 gagttcttaa aggtatgcat tcctctggcc tcttacgtct tcttctagga ctgttgacga
      961 tctctgtatc atggatgatc aatccacata attcgtataa tttatggagt aatgatccat
     1021 aaaaagcatt caaggtggta ttatttaatt ggtgatgaag atggattttt cgacagtagc
     1081 attttacaat ccatggtggg acgaaaaaag caagaataca gacttaactt tccatctagt
     1141 tgacaagtac gagagtagct cattcaagag agactacaac gggttttttg atcttactgg
     1201 aaataacctg tatgtcgtca ggggtccacg taagattgga aagtctactc tgctgaagtt
     1261 taccatagca aaactcctga aatcgggaga gagacgctca gtgctctatc tcccactgga
     1321 tacaatatcc agagtagatg agctgagaag ccttttgttt caatatcttc aattcaccag
     1381 aaacgagaag aagcgatatg tatttattga tgaaatcacg atggtcgatc agtggcaacg
     1441 agcagtcaaa gaggttcgag acaatacgga gatggtcaat gacttctttg ctttaactgg
     1501 ctcctccgca attgacttaa agaggtcatc tgaaaggctt ccgggtagaa aagggcaaag
     1561 cgggaaggat attctacttc ttccgttgac tttcagagag tacctgagct gcaagaaagt
     1621 tgagggactt ccgaaatgtc aacccgagga cattctaaaa atggtcccgg cagacgcctt
     1681 tgaactgctc attaaggacg aaattctcaa gactgaattc ttcaactatg ttgcttcagg
     1741 aggattcccc tctgcaattg aagcttattc aaaaggtgaa agtcgcgaaa cgttaattaa
     1801 cactttctgg gagatcctca taggaaacat agagaggtat ggtctttcaa gagcgaaact
     1861 catccagata cttacctatg tctctagcag actttcatca agattctcct ggagcagtgc
     1921 ggcaagcgag acggaaatag acacaaagac atttcaaaaa tacgttgaag tattgggaat
     1981 cgactactta cttctaaccc tcaagcacat ggatcaaaaa acacatctac cttcagaaaa
     2041 gaaacagaag aagatatatt actgcgacag actaatctca gatgtagtat cgcaaaagct
     2101 tggtgttcag cttgagagaa gcgcaatact ggagaacatc atgacgacca actgcgcatt
     2161 cgctttcgga aagaaccttg ataatggact tgattcgatt actgacttcg gttactggta
     2221 ttcaaaagaa ggaaaggaaa tcgacttgct agtaaaagac gttccggtcg agctgaagta
     2281 tcagaacaac ataaccaacc aggatctatc gaccataaaa agacacttca aaaaggggat
     2341 catcctttcc aagcatacta ttgatctttc tggagatata aaaatactgc ctcttcacat
     2401 ctttctcgcg attactggac agtaaatcct tccgggatta cccccggaaa aaatggggac
     2461 tgacaaatct tctaatgtct gtacccattt ttgcgaggga cagggattct gaccagaagc
     2521 acgtcagatg acatgaggcg agagtatcct gccgcgaaaa catgttgctt catccagtat
     2581 atccccactg tcatcccaca gtgttcctga gcgggatctc agtccttctc ttccttctca
     2641 cctctccctt ttctctcggg ctcattacga aggaaaaaag aacctggacg cgacgtgtcg
     2701 aagaatcgtt cttccctgcg taaagcggtc agtgtttctc atccactcgg ctccttcccc
     2761 gcgaagcggg ccttgcgtcc tggcatgatt tttgccagcc ctgcgtcccg ggatgtactt
     2821 cccggccttg cgtccgccga cgatcttcgg cgccttgcgt cttgcaactt ctagtcggaa
     2881 gacggagaac cggtgactgc tgacagcaaa tctgttctaa tgaatcaaga actatattac
     2941 aggaaggtaa ataatggagt ctgtcccgaa aaacacgaaa ggttcgtcat tcttctcgtc
     3001 cttatcgccc tctctctttt ctctcgggtt cattaagtaa gaaaaaagat gctaggcgtg
     3061 aagtgtcgaa gaatcgtttt ccccagcgta cagccttcag cgtttgttgt ccaagcggct
     3121 ctttccccgc gaagcgggca ttgcgaccgc cgatgctctt cggcgcattg cgtcttgcaa
     3181 cttctagtcg gaggacggag aactgatgac ggttgacctg agaggctcct tgcgaagcag
     3241 catcacttct tgaattaacc ctcgccgctc cggtgtttga aggacctgca agcaagagta
     3301 tgcaaattct tgagcaaatg ctcagtgaca atcacgtgtg ttaattcgtc atagtgattg
     3361 atataattca tgttgagtaa gaggaggtat tttgttgaaa gaacgtcgtg gcatttttga
     3421 actcgttgac ctgaattcgt ggagggaatt aggagtggct gcgcccggaa gaaatgagaa
     3481 gtactggttt gtcaatcatt ttggcgagga gtggctcttt aagataccga aagtgggaac
     3541 aacagaacat gtatctgaga aactggctta tgaaattgct aagcttgttg gaattgaagc
     3601 tgcggagact gagtttgcaa tatataaggg gagactcggt acagttagta agaacttcgt
     3661 agaagtcgat aagggagaga acctgataga gatgttggat ctgattcaaa agatgcaacc
     3721 aggataagat cccgaattaa tgaaagatac gttgacaggt agagagtatt ctctggagct
     3781 tgtaatagac gtaatccggg caacaaggga agcacttaca accaaagtta tgcagtattt
     3841 aatttttgat gctttgatag gtaacagcga tagacaccat gggaactggg gaataatata
     3901 tggtggtctc ggagaaataa gaatggcacc ctcatatgat cacagtgcat cgcttggttc
     3961 gaagataagg gaagaggaca ttgagaatat tctgaaggat aagagacgct tcatggcaaa
     4021 tgttgaaacg aaggcaaagt cactattggt attctccgga aaaagaaaag tcacacataa
     4081 agaattgctc atttacataa gagacaattt tgctgacagt gagttagtta gtacaatact
     4141 tcgcatggca agcaagatta acagaaccaa cgtgaatgat ataattgatc tagtaccttg
     4201 cgagatactt agcagaacaa cgaaagactt attgctggaa ctgattatcg caaaaaggca
     4261 actggtagag gaagtgtttg gatgagaaaa gccgtgcttt attatagagc aaaaccagac
     4321 aggaagattc ccattggatt cttggtcttt gatgggaaac attattcgtt cgaatatgat
     4381 gagactgctc tgaagaactc agagacttca agcttgatcg acatcctacc gtttagtagg
     4441 cagactgtca catatagcaa caagcttttt cctttcttct cgagacgttt accagataag
     4501 aagagaaggg actaccatac cattcttgat agatttggga tcaggaacaa tgctgagctt
     4561 gagctactat ttgttaacaa cggcagatta ccgaccgata acttcgagat taccgagatc
     4621 agatagtctt caaaagaaca ttctgttctg acaatgtgct gtgccccttt gcatatctgc
     4681 tgttcactta accacaattc ttgacgtgcg tcatcagaac gaaagatccg ccctacgctt
     4741 aaaagacgga aatccgctga tcgctgtaaa gacaacatta tccgtccacg gaccgccgtc
     4801 ctccgagaaa agagttcgct tctctccagt ctcttcctct ctcctattct catagttctt
     4861 aacaaagaac ctgtacg
//
LOCUS       DAOQYI010000090         4740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_18306_length_4740_cov_74.859125, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000090 DAOQYI010000000
VERSION     DAOQYI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18306_length_4740_cov_74.859125"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /gene="nusB"
                     /locus_tag="PKL93_10805"
     CDS             complement(<1..353)
                     /gene="nusB"
                     /locus_tag="PKL93_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNQ71788.1"
                     /translation="MTAGPNKSRRKMREIVFSAIYQFDFNEDMETSSEYLERELSFFS
                     METEMKLRTRKYFDGILRYRDEIDSVIRKHLTNWTFERLASTDRNVLRLGAYEIIYEP
                     DIPIEVTLNEAIDIA"
     gene            complement(358..816)
                     /locus_tag="PKL93_10810"
     CDS             complement(358..816)
                     /locus_tag="PKL93_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNQ71789.1"
                     /translation="MDFEETDLGRIEISEAVIRDIAIHSYMEFLKFDPKEGKARKEAK
                     STVDIDLDEKVDGTKTVKINVNTKIKYGVSIPSYARKMQEKLKNDVESFSGIKVEDVS
                     ITIEDVYEEAQKPAVIEEEEEFESVEAKPELEEMASQDSTVEEEDREKEG"
     gene            complement(836..1393)
                     /gene="efp"
                     /locus_tag="PKL93_10815"
     CDS             complement(836..1393)
                     /gene="efp"
                     /locus_tag="PKL93_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNQ71790.1"
                     /translation="MIEVGKIRKGMPLIIEEEIYIVTEANKHSMGRGDGIIRTKMKNL
                     KTGLVRQFTFQASEKVEEAALSFRHVQYLYSEDELFHFMDLETYEQYTIGVDEMGDAL
                     DYIKENEEVDLQFHEDRPIGVILPNTVVLKVTDTAPAYKGDTVSGSGKPAVLETGLKV
                     SVPFFVENGELIRVDTRTGEYIERA"
     gene            complement(1415..2491)
                     /locus_tag="PKL93_10820"
     CDS             complement(1415..2491)
                     /locus_tag="PKL93_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNQ71791.1"
                     /translation="MSRIEQFREKLKERNLDGFVLYNMESSNRSSSWYLSGFSGSFSV
                     LIITPKSEYIVTDSRYFTQVEMESKFKLIPYKNGDELKDIIKAALLDDGANHVGFEEE
                     TISFGLYKRVFSHLGVELEPADSILKDMRMVKTADEVEKIKAAVKVAEDALTETLNFI
                     RPGRTEEEICATLEYEIRKRGGYLAFETIVGSGPRSAIVHGRPSKRKLQIGDFLLIDF
                     GARVDGYNSDITRTFSIGNVTDEMVKVYETVVKAQTEAKRVAKAGVIGSYVHELAASI
                     IEEAGYGDYFGHGLGHSLGMDTHDGQGLSPKNSAPIPAGAVITVEPGIYLPGKFGVRI
                     EDDCYFRENGIDVLTNLDRELKIL"
     gene            complement(2527..>4740)
                     /gene="mutS"
                     /locus_tag="PKL93_10825"
     CDS             complement(2527..>4740)
                     /gene="mutS"
                     /locus_tag="PKL93_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNQ71792.1"
                     /translation="QTEDPALAKGLVKREVTRVVTPGTIVEDELLPEAENNYIIAIGK
                     SGEFFIIVLADVSTGEVIISEAEDLISLKDFIVASQPSQVLLRESLKDLKKEIAGVTS
                     SMVELIEDWHFALSSGINYVKEFYSITSIDHLELDDKQVEVLGALFKYLETINFSPLK
                     HLSLPRILRKTDSMQLDASTVENLNLFRLERKGSLLEILDETITGMGKRRLRQWLLSP
                     LTDRHKIDERLDVVQALYSDRHLLDELREYFHSVYDIERITSRLSTERITPRDLQSLR
                     TSLAVLPYIKELISNETAFEGFCERFELFPDELELLRRSIVDEPSSSIGDGNVIRKGF
                     DAQLDSLRDLLDNSVEKIRAIETSEKKSTGIPSLKVKFNKVFGYFLEVPKSQSGKVPE
                     SYTRKQTLVNSERYITPELKEFEDKILSANEKIQSLEKALFLCVCSQLMNSLQRLKDA
                     AIVLSEIDALQSLSLVARKYNYCRPRFSVDGSARISNSRHPIVERFVPDFTPNDISMD
                     SSENFFIVTGPNMSGKSTFIRQVALLSIMAQMGSFIPAKDAMLPIYDRVFTRIGARDE
                     LASGKSTFLVEMMETATILSKATEDSLVILDEVGRGTSTFDGISIAWAVSEFIYEAIG
                     CHTIFATHFTELTELSSMYPGIKNKTARIVETEKGIIFLHKVIDGVANSSHGIEVAKL
                     AGVPETVLSRAKEILRVISKQSALDKAVRVLTAEDLREIRQSKKGKMNRNQISLF"
BASE COUNT         1184 a         1149 c          921 g         1486 t
ORIGIN      
        1 tttgcgatgt ctatcgcttc attaagagtt acttcaattg gaatgtcagg ttcataaatt
       61 atctcgtatg ctccgagtct gaggacgttc ctatcggtcg aggcaagtct ctcaaaagtc
      121 cagttcgtga ggtgttttct aataacacta tctatctcat cacgatacct caaaatgcca
      181 tcaaaatact tccttgtcct aagcttcatc tcggtttcca ttgagaagaa actgagctct
      241 ctctcgagat attcagacga agtctccata tcttcattga agtcaaactg atagatcgcg
      301 ctgaaaacaa tctccctcat ttttcgtctg cttttgtttg ggcctgccgt caccttttca
      361 accctctttt tctctatctt cttcttctac ggtagaatct tgtgaagcca tttcttctaa
      421 ctcaggtttg gcttctacgc tttcaaactc ctcttcttcc tcgatgacag caggcttctg
      481 ggcttcttcg taaacatctt caattgtaat cgacacgtct tcaaccttta tgccgctgaa
      541 gctttctacg tcgttcttca gtttctcctg catttttctg gcgtacgagg gtatcgagac
      601 accatacttg atttttgtgt ttacgttaat cttgacggtc ttggtcccat cgaccttttc
      661 atcaagatca atgtcaactg tcgacttggc ctcttttctt gcttttcctt cctttggatc
      721 gaacttgagg aattccatgt aggagtgaat agcaatatcc cggattacag cctccgagat
      781 ttcgattctt ccgagatcgg tctcttcaaa atccatcatt ctccctcctt tccacctatg
      841 ctctttcgat atattcgcct gttcttgtat ctacacggat cagctctccg ttctcgacga
      901 agaatgggac agaaaccttc aatcccgttt cgagaacagc cggtttgccg cttccagaaa
      961 ctgtatcacc tttataggca ggtgcggtat ccgttacttt gaggacgaca gtgtttggaa
     1021 gtataacgcc tataggtcta tcttcatgga actgaaggtc cacttcctcg ttttccttga
     1081 tgtagtccag agcatctccc atctcatcta caccaattgt atactgctcg taggtctcaa
     1141 ggtccatgaa atgaaagagc tcatcctccg agtaaagata ctgaacatgt ctgaatgaaa
     1201 gcgcagcttc ttcaactttt tcgcttgctt gaaacgtgaa ctgcctaaca agaccagttt
     1261 tcagattttt catcttggtc cttattatac cgtcccctct tcccattgaa tgtttgttag
     1321 cttctgttac aatgtagatc tcttcttcaa ttattagggg cattcccttt ctaatctttc
     1381 caacttctat catctagaag ccacctccgg atcgctaaag aatcttcaac tctctatcta
     1441 gattagtcaa aacatcaatt ccattttcac gaaaataaca atcgtcttct attctaaccc
     1501 caaattttcc gggaagatag atacccggct caactgttat gactgctccg gcaggaatag
     1561 gagcagagtt ctttggagac agaccttgac catcgtgagt gtccattccc aggctgtgcc
     1621 caagaccgtg tccaaagtaa tctccgtaac cagcctcttc aattatactt gcagccaact
     1681 catgcacgta actgcctatc acgcctgcct tagcgactct tttagcttct gtttgggcct
     1741 taacgactgt ctcgtaaact tttaccattt catccgtcac attgcctata ctgaaggttc
     1801 ttgttatgtc agagttgtaa ccgtctaccc ttgcaccgaa gtcaatcaac agaaagtctc
     1861 cgatttgaag ctttctcttg gaaggtcgcc catgcactat ggcgcttctc ggaccgctac
     1921 ctacaatagt ttcaaaggcc agataacctc cgcgttttct gatttcatat tcgagggtgg
     1981 cacaaatctc ttcttccgtc cttcccggac gaatgaagtt gagagtttca gttagtgcat
     2041 cttctgcgac tttgacagcg gcttttatct tctctacttc atcggcagtt ttaaccattc
     2101 tcatatcttt gagaatagag tctgcgggtt ccaactccac tccaaggtga gagaaaaccc
     2161 ttttgtagag gccaaaacta atagtctctt cctcgaatcc gacgtgattg gctccatcgt
     2221 cgagtaaagc ggccttaatt atatccttaa gctcgtctcc gtttttgtaa ggaataagtt
     2281 tgaattttga ttccatttca acttgagtga aataccttga gtcggtcact atgtactcac
     2341 tcttaggtgt tatgattaga actgaaaagg agcccgaaaa gccagataga taccatgaac
     2401 tggaacggtt gctagactcc atgttgtaca gcacaaaacc gtcaagattt ctctccttaa
     2461 gcttttcacg aaattgctca attctcgaca tgatcaacct ccggattgcc aaattctttg
     2521 tgtaccctaa aacaaactga tttggttcct gttcattttc cctttctttg attgtctgat
     2581 ttccctgagg tcttcggcgg ttagcactcg cactgctttg tccaatgccg attgtttgga
     2641 aatgaccctt agaatctctt tagctctcga aaggacggtc tcgggaacac cggccagctt
     2701 tgccacttct attccatgag aactattcgc tacaccgtcg atcaccttat ggagaaagat
     2761 aattcccttt tccgtctcaa ctatgcgagc tgtcttgttc tttatacctg gatacatgct
     2821 agagagttca gtaagttctg tgaaatgagt tgcaaatatc gtgtggcaac caatcgcttc
     2881 atatatgaat tccgaaactg cccatgcgat tgaaatccca tcaaacgtgc ttgttcctct
     2941 accaacttca tccaggatta ctaagctatc ttcagtggcc ttagatagaa tcgttgctgt
     3001 ctccatcatt tcaacaagaa aagttgattt gccgctggca agttcgtctc tggcgccaat
     3061 tctggtgaag accctgtcgt aaatcggtag catcgcatct tttgccggta tgaatgaacc
     3121 catttgagcc attattgaaa gcaaggctac ttgtctaatg aaagtggact taccgctcat
     3181 atttggccca gttacgatga agaagttctc ggagctatcc attgatatat cattcggagt
     3241 gaaatcaggc acaaacctct caactatggg atgtctagaa ttcgaaatcc ttgcacttcc
     3301 gtcaacggaa aagcgcggcc ggcagtaatt gtattttctg gcaacaaggg atagtgattg
     3361 cagtgcatcg atctcagaca gaactatagc tgcatctttg agcctttgca acgaattcat
     3421 aagctgcgag cagacacaaa gaaaaagtgc tttctcaaga gattgtattt tctcgtttgc
     3481 actgaggatc ttgtcttcga attccttgag ctccggagtt atgtatctct ctgaattaac
     3541 caacgtttgc tttcttgtgt agctttcagg gacctttccg gactggcttt ttggaacttc
     3601 gagaaagtat ccgaaaacct tgttgaattt caccttcaac gaagggattc ctgtagattt
     3661 cttttcgcta gtttcaattg ccctgatctt ttcaacggag ttatcgagaa gatcacggag
     3721 agagtccagc tgagcgtcga acccctttcg gataacattt ccgtctccta tggaggaaga
     3781 gggttcatca acaatactgc gccgcagcaa ttccaactca tcgggaaaaa gttcgaatct
     3841 ttcgcagaat ccttcaaatg ccgtttcgtt agaaatcagc tcttttatgt atgggagaac
     3901 agcaagagag gttctgagag attgaaggtc tctcggtgtt atcctttccg tcgagaggcg
     3961 actcgttatc ctttcgatgt cataaacact gtgaaagtat tctctcaact catcgagaag
     4021 gtgacgatcg gaataaaggg cttgaactac gtctagcctt tcatcgattt tatgcctgtc
     4081 agtcaagggc gaaagcagcc actgcctcag ccgtcttttt cccatccctg tgatcgtctc
     4141 atcaagtatt tccaaaagag aaccctttct ctcgagacga aagagattaa gattttcaac
     4201 tgtagaagca tctagctgca tcgaatccgt ttttctgaga attcttggga gtgaaagatg
     4261 cttcagggga gagaaattta tcgtttcaag gtacttaaac aaagcgccta gaacctcaac
     4321 ttgcttatca tcaagttcga gatggtcgat agaggtaatc gaataaaact ccttgacata
     4381 gttaatgcct gaagataacg caaaatgcca atcctcaata agttcaacca ttgatgatgt
     4441 aacgccggcg atttccttct tcagatcttt caagctttct cttaagagaa cctgagaagg
     4501 ttggctcgcc acaataaagt ccttgagcga aattaggtcc tctgcttcgg aaattatgac
     4561 ttcacctgtc gatacgtcgg ccaatacgat tatgaaaaac tctccagact tgccaatagc
     4621 gattatgtag ttgttttcgg cttcggggag cagttcatct tcgactatcg ttcccggcgt
     4681 aactactctc gtcacttctc tcttaacgag tccttttgca agggctgggt cctctgtttg
//
LOCUS       DAOQYI010000091         4739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_18315_length_4739_cov_84.851196, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000091 DAOQYI010000000
VERSION     DAOQYI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18315_length_4739_cov_84.851196"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            858..1454
                     /locus_tag="PKL93_10830"
     CDS             858..1454
                     /locus_tag="PKL93_10830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ71793.1"
                     /translation="MTFDQWKEELEKLSNEGPFAIADIDIDKFSLLNKEYGRDCGDRV
                     FEILDKTLKENLPEKLSFVRFGDEFFVYSSEYTLETLFMEMQDLAQTISKLSFECKGK
                     TVRFTVSGAVGEFPRNASTVEKLLNLLSEGMRKAKVTGRAQIIFAPLEKESKMVMKSN
                     YYLRSQLDGLAKLTALLNRTESSLLREALDDLLRKYKL"
     gene            1519..2736
                     /locus_tag="PKL93_10835"
     CDS             1519..2736
                     /locus_tag="PKL93_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ71794.1"
                     /translation="MREKMNRNISVYYRFKFSQNMLIIGPVLVPYMLFKGLSYSEMMF
                     LQSISAVAVVLFEVPTGSIADRVSRKFSLVISSVITAFGLSLYIVCESFLAFALAELL
                     FGLGMTFSSGADSAILYESLSRMEREKEYSKIEAKAMSNIFVGQAVGSIASGLLYSVS
                     PYIPFWISLGMLFLSSGYSLFFTEPDHGERSRESYLVHVARSFGDAVKKPRVRWALYF
                     SALMGVMLRASFWLYEPYFKLVRLDIALYGIVFAGFNVVCALSSRYLMKLFENVRPRK
                     SLTFLGLLMGGAYLMPLILLNVGSLAFLALGQIVRALYQPIMRFYINRQIEDAFRATI
                     ISIVSLTASLSFAVFSPLIGLGLDRAGAISVYLVMGLVSVGGSLFLWRVRNLQKRKKA
                     AADRKSALDETVS"
     gene            complement(2826..4331)
                     /locus_tag="PKL93_10840"
     CDS             complement(2826..4331)
                     /locus_tag="PKL93_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ71795.1"
                     /translation="MKKGKFLLLVVLLLSILSFSTTIKMYLVTDYHSHALPFYSEGQH
                     GFGGIAKIIAFLKEKAGDEDTLVFGGGDMINLGTPAWSDKFHAIEMPWFNNIFDAMAY
                     GNHDSEYGPADFQEVWKNVTYPILGANVLDASGVPVFQYFGKRYLILEVEGKKIGVFA
                     LAGSDFDSLVKPAARPVEGATFGDFMTTAGEIVDEMEAEGVDLIVFIGHAEYEETLKL
                     AREIEGIDLIFGTHSHLKIPLTKIEGTETYFISPYQYGTYAAEVVVYFGADGQNSVSG
                     SLIPMDDSMPEDPVIADKVEKLQYELESDPEFAHLFEWIGDVETELSVANVNTGESVL
                     GNFVMDIIREKSGANVALSTSSSFRASIAPGSTVYEDLKNALPYVNTIYVYEVKGETL
                     EKILNHSVSQSGTGFFSQVSGVRFIISNGKATSIEVQADLECPDNFTLLNPEATYLVA
                     TTNYQGLFAPGYKDLFAGLPYTDTGLDVQKIVKEYMQNNSPVSARLDGRIR"
BASE COUNT         1236 a         1117 c         1158 g         1228 t
ORIGIN      
        1 ctgttgacat agtagaagcc catcgtccaa gagcaaggac gcgtccttcg aagagaagtt
       61 gcaagttgaa agcgctggag tgaagaaacg tcaaatgccg tcattccgac aaagctcctg
      121 gtcggaatct cgatgctatt gtttcattgc tctaaccaag aacgaagaac ttggacgcgg
      181 agcgtcggaa cgaggaacaa gctttttctt cggcgaacgc agagccattc agcggtgatg
      241 cggtttcact ctggtctttc aaacgtgcaa cccgctaacc tcggccatgg ttttcaaaaa
      301 accagatccc ggatcaagtc cgggatgaca gacttaggtt attcggaaaa catcacatca
      361 catgacagcg tcctgtaact cttaaaaaca caacaacctg tcattctgaa cttgattcag
      421 aatcttggtc ttgagagccg ctggacactt gctaagaaaa gctgatggct gatcgctgtg
      481 aaaaaccatc cttagtcttt catgaagagc agtagcaagt tgaaagcgct aaagtgaaga
      541 aacgtcaaat gccgtcattc cgacaaggct cctggtcgga atctcgatgc tactgcttct
      601 ttgctctaac caagaacgaa gaacctggac gcggagcgtc ggaacgagga acaagctttt
      661 tcttcggtga acggagaacc gctcaacggt caaccagttt cttttctctt acgaaccccc
      721 aaccacctac cccgaacctc gtcatttatt cttccgcgtc tggcttgcaa gacttaacct
      781 tccggaaatc ggatataaat acacttggtg ataatatatc catatacata tatcagtata
      841 tacataggag gatatttatg acttttgacc agtggaaaga agaactcgaa aaactctcaa
      901 acgaaggccc ctttgctatt gcagatatag atatcgacaa gttttcgctt ttgaacaagg
      961 aatatggaag agactgcggg gacagggtct tcgagattct agataagacg ctgaaggaga
     1021 atctcccaga gaagctctcg tttgtcaggt ttggagacga gttttttgtc tacagcagcg
     1081 agtacaccct tgagactctg tttatggaga tgcaggattt ggctcaaact atttcgaagc
     1141 tctcatttga atgcaaggga aagacggtta ggtttaccgt cagcggtgcg gtaggagagt
     1201 ttccaaggaa cgcgtcgacg gtcgagaaac tgctgaacct cctttcggag ggaatgagaa
     1261 aggcaaaggt cacagggaga gcacagataa tctttgcacc gcttgaaaag gaatcgaaga
     1321 tggttatgaa atccaactac tacttgagat cgcagctcga cggactagca aaactaactg
     1381 cccttttgaa cagaaccgag tcatcactct tacgggaagc gctggatgat ctactgagaa
     1441 aatacaaatt gtagaaatct caaaataccg aagagagggt ggccggggag ccaccctttt
     1501 tgtttaggag atgatctcat gagggagaag atgaatcgaa acatctcagt ttactaccgt
     1561 ttcaagtttt cacagaacat gctcatcatt ggtccggttc ttgttcctta tatgcttttc
     1621 aaggggctga gttattcgga gatgatgttc ctgcagtcga tctcggcagt ggcagttgtt
     1681 ctcttcgaag tgccgaccgg ttcgatagcc gacagggttt caagaaagtt ctccctcgtg
     1741 atttcatcgg taataacggc cttcggactg tctctgtata ttgtctgcga aagctttctg
     1801 gcctttgccc ttgcagagct gctctttggg cttggaatga ctttcagctc aggcgcggac
     1861 tcggctatac tttatgagag tctttcaaga atggaaagag agaaggaata cagcaagata
     1921 gaagcgaagg ccatgtcaaa tatcttcgta ggtcaggccg ttggatcgat agcaagcggg
     1981 cttctatatt cagtttcacc ttacattccc ttctggatat ctctggggat gctgtttcta
     2041 tcgagcggct attccctctt cttcacagag cctgaccatg gagaaagatc aagagagtcg
     2101 tatctggttc acgttgctag gtctttcggc gatgccgtaa agaagccgag agtcagatgg
     2161 gccctatact tctcagcgtt gatgggagtg atgttgagag caagcttctg gctgtatgag
     2221 ccgtacttta aacttgtcag gctggatatt gccctctacg ggattgtctt tgccgggttc
     2281 aacgtcgttt gtgcgctttc gtctaggtat ctaatgaagc tcttcgaaaa tgtcagaccg
     2341 agaaaatcgc tgacattcct tgggttactt atgggaggcg cgtatctaat gcctctgatt
     2401 cttctgaacg tcggttcgct tgcatttcta gcgcttggtc agatagtcag ggctctatac
     2461 cagcccatca tgagattcta tatcaacagg cagatagaag atgccttcag ggcgacgata
     2521 atctcgatag tgagtctaac cgcaagcctc tcctttgcag tcttttcgcc gctaatcggg
     2581 ttggggctgg acagggcagg cgcaatttcc gtttatctag taatggggct tgtttcggtc
     2641 ggcggatcac tgttcctctg gagagtcagg aatctccaga aaagaaagaa ggccgcggcc
     2701 gatagaaaat ctgcgctgga cgagacagtt agttaaactg aaacgggtca gaagtccggg
     2761 agactatatt tcttgcacaa gagaaaaccc ccacctgagt gggggtttgt acttgcggtt
     2821 atctattacc taattcttcc gtcaagtctg gcgcttaccg gactgttgtt ctgcatgtac
     2881 tccttgacta tcttctgtac atccagtcca gtatcggtgt atggaagccc cgcaaagaga
     2941 tccttgtaac cgggagcgaa gagtccctga taattcgttg tggctacaag ataggttgcc
     3001 tcgggattca gcaaagtaaa gttatccggg cattccaggt ctgcctggac ctcaattgac
     3061 gtcgcctttc cgttgctgat tataaatcgc acaccggaga cctgagagaa gaacccggta
     3121 ccgctctggc taactgaatg gttcagaatc ttctcaagcg tttctccctt tacctcgtag
     3181 acatagattg tgttgacata aggaagggcg ttcttcagat cctcgtaaac agtcgatccc
     3241 ggtgcaatag atgccctgaa gctgctcgaa gtagaaaggg cgacgtttgc ccctgatttc
     3301 tctctaatga tgtccatcac gaaattgcct agcaccgatt ctcccgtgtt cacattagct
     3361 acagatagtt cggtttcaac atccccgatc cattcgaaca ggtgagcgaa ctcaggatcg
     3421 ctttccaatt cgtattgaag tttttctacc ttatccgcaa tgactggatc ctctggcatg
     3481 gaatcatcca tgggtattag cgaccccgag acggaattct gaccgtcggc tccaaagtaa
     3541 accactactt ccgccgcata tgtcccatac tggtaaggcg aaatgaagta tgtttcagtt
     3601 ccctcgatct ttgtaagagg gatcttgaga tggctgtgtg taccgaagat caggtctatg
     3661 ccttcgatct ctcttgccaa tttcagtgtc tcctcgtact ctgcatgtcc aatgaagacg
     3721 ataagatcga ctccctctgc ctccatttcg tcaacgatct cacccgcagt cgtcataaaa
     3781 tctccgaaag tcgctccttc gacgggcctc gcagctggct tgacaaggct atcgaaatcc
     3841 gatcctgcaa gagcaaagac accgattttc ttgccttcaa cttctaggat caaatacctc
     3901 ttcccgaagt actggaatac aggtactcca ctcgcatcga gaacgttggc gccaagaatc
     3961 ggataagtga catttttcca tacttcctga aaatctgccg gtccgtattc cgagtcgtga
     4021 ttgccgtaag ccatggcatc gaagatgttg ttgaaccagg gcatctctat ggcgtggaac
     4081 ttgtcggacc acgccggggt cccgaggttt atcatgtctc caccgccaaa gacgagagtg
     4141 tcttcatcgc cggctttctc tttcagaaaa gcgattatct tggcaatccc cccgaacccg
     4201 tgttgtccct cagaatagaa gggaagtgcg tgggagtgat aatcggttac gagatacatc
     4261 ttgatggtag ttgagaacga aaggattgaa agaagcaaaa caaccagtaa aaggaattta
     4321 ccctttttca ctgtcaacac ctcctcgtgg tagtcaagcc aattctagca ccgttaggtt
     4381 aagtcaaatt tctccaggta aatatattgc cttaaagatt gatgaggcaa agggcatcgc
     4441 tggaagtaag gctcgctagc gcgcggaagt gatgttacta caatcagacg caaagcgccg
     4501 aaaaagatcg gcggtcgcgc aatgccggca aagatcgtgc cgggacgcaa tgcactgaag
     4561 accatcagtt gacgcaatac tgccttcggc aggaagcctt ctcaggttca ccatcaaagg
     4621 ttctccgaga aaaataccgg ttcttcgttc cgacgctgcg cattcaggtt acaggaaaaa
     4681 ccaagatcag aaggttgagg cgggagattt acgcgaggga caagacgttc gctgcgctc
//
LOCUS       DAOQYI010000092         4687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_18586_length_4687_cov_74.669689, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000092 DAOQYI010000000
VERSION     DAOQYI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18586_length_4687_cov_74.669689"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(817..1638)
                     /locus_tag="PKL93_10845"
     CDS             complement(817..1638)
                     /locus_tag="PKL93_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71796.1"
                     /translation="MRKYLFSAGIVLFSVLFFAGCFSLNGTPDKVKISVEAVMNDWNL
                     LFSNSLELISWDYVGIIEDYDDLKETLELLTDPDSVRLFQIDIMDPSFASIILLSSIP
                     SADQDEFAEAVEELVELGQHKVKLNWRTVGSGSEFTTIALVDENNIVWDSIIGSVVVE
                     EEFLPVAGEKTGSVIIKWLWGAERGKISWSLECSGPPLACNYTSESKISSGEARIETK
                     LVKGEECCTLYYAWAYRTPTGSISIDWNPETEQFDVEVSSWGSTGSGQGIFECCP"
     gene            2146..3783
                     /locus_tag="PKL93_10850"
     CDS             2146..3783
                     /locus_tag="PKL93_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNQ71797.1"
                     /translation="MFSDMIVDLMFVEKDLVAHAQTYKGWAFVIFSGILFYFLIYREF
                     SEKNKTQLELVKQKDLSDAVLDTVGVFVAVLDSEGVIVFTNETFEEILSLKSEDIIGK
                     NCSEVFASPDLANWIENTASKTLDREIENFYEADLETSSTTLHIRWALSNLTSWRGEH
                     DYFVLTGVDITQLVESERSATHRLANIRALHEIDMAVSYHLELEKMLDVFLERLISRL
                     GVDGADVFLIDEERSVLRFAHGKGIVTGEMDFRELPMEGTIPSSVASSGKPYSGPLDT
                     SSNPECPRLKNLIDMKVKDYHAVPLETRGKILGVLETFDMMQIHRDSEWNDFLQMLAA
                     QASLAIDVALMIDNLKDSNRKIAEAYDQTLEVLVGTLEMRDMNTKKHSRRVADLTVWL
                     AEKMGASEEEIENMYRGALLHDIGKISIPDSVLLKEGPLSEDEWKVMKTHPTTAYEVL
                     SQVEYLRPSLDIPYCHHERWDGSGYPRGLKGEEIPLAARIFAVVDVYDALTSDRPYRK
                     AWSKEKTVEYLLENSGKLFDPAVIEKFLEILRVSSPS"
     gene            complement(3807..>4687)
                     /locus_tag="PKL93_10855"
     CDS             complement(3807..>4687)
                     /locus_tag="PKL93_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HNQ71798.1"
                     /translation="VGDHKYDAGKSLTLQATPAEGWLFEHWLVEDAVYSSEHEAELTM
                     NTDRNVKAVFVRKTHTLTMLEPSGSGNVEPAVGISIYKEGSVVTLVATPSNGWDFDFW
                     QIDGALFSYNSQITITMNASKTAKAFFVEEPPTVFTLTMVEPIGQGSVSPPVGDHTYI
                     EGTSVPLSATPADGWEFEAWYLDGTFYSDSETTSIIMDRDKSVSAEFVQKAEKVTLEI
                     YKYGRGTVSPPEGTYVYDKGSSVDLSATPDSGYHFEEWLVDGTTYEVPEITITLEWDT
                     IAQAVLRCNCGGSCEK"
BASE COUNT         1315 a         1063 c         1064 g         1245 t
ORIGIN      
        1 caagagaaac tggttgaccg ctgagcggtt ctccgtttgc gagaagagcc tgaagcagta
       61 acatcgagat tccgaccagg agctttgtcg gaatgacgga atctggcctt ttcacaataa
      121 ccgttagttg tcatcccgaa cttgtttcgg gatctgggct tacgcaagtg gtcggaggta
      181 gcagtttggg ggtcggaaag ggcaaaggat cggtcccagg atgcaagttg taagttgtaa
      241 aaaaggctgg ggtcggagca tggcggaatc cctgacgaag aactaagaac ctggacgcgt
      301 agcgtcggaa cgaagaaacg gtattcttgg cggggacgga ggaccgttga cggacaacgt
      361 tgtcttcaca gcgatcagcg ggtttttgct cttaagcgca cagcggttct ttccttgcaa
      421 ctaggaacag tcaacttgca actgctcttt cgaaggacgg gttcatgatc ttggacaaag
      481 gaccaaggac catcctcact ccagcatgat tagatatgat gtctgatgtt agtttttaaa
      541 cccttgtcct cacctatctg gcgtctagat tgctttccat aagtgcttca ccacaaaacc
      601 tttcaggagc gaaactcaaa aagtcgctaa cttaatgaag aaagagcgtt gtctatgata
      661 atcaaaattc tgaaactaag gatagtactt cggaaaaact cactccaatt cttgtcactg
      721 aatttctgcg attggacaaa tgaatggttc ttttgaaaga gatgacttcg aatacttgca
      781 gcaagaaaat tctataatca ttcgtcaaca atcaactcat gggcaacact cgaaaatccc
      841 ctgtccgctt cctgtgcttc cccagctgct aacctctaca tcgaactgct cagtttctgg
      901 attccagtcg atgcttattg aacctgtcgg agttctgtag gcccacgcat aataaagtgt
      961 acagcactcc tcacccttaa ctagcttggt ctcaatgcgg gcttcacccg aagaaatctt
     1021 tgactctgaa gtgtaattgc atgccagagg tggaccgctg cattcaagac tccagctgat
     1081 cttgcctctc tctgctcccc agagccattt gatgataacg ctgccagtct tctctccagc
     1141 aaccggtaag aactcctctt ccacaacgac acttccaatt attgaatccc agactatgtt
     1201 attctcatcc actagggcga tggttgtgaa ctccgaaccg gaaccaacag ttctccagtt
     1261 caatttgacc ttgtgctgtc caagttcaac aagctcctca accgcctcgg caaattcgtc
     1321 ctggtcagca gacggaattg aagagagaag aattatcgat gcaaaggaag gatccattat
     1381 gtctatctgg aaaagccgaa cggaatcggg atctgtaagc aactcaagtg tttcctttag
     1441 gtcatcataa tcctctataa ttccaacgta atcccatgaa atcagctcta gtgaattcga
     1501 gaatagaaga ttccaatcgt tcatgacagc ttctacggag atcttcactt tgtcgggagt
     1561 tccatttaaa ctgaaacacc cggcgaaaaa caaaacactg aaaagtacta ttccagcgga
     1621 aaaaaggtat ttcctcataa caatcgtctc ctttatcccg atcgtttctc tcctatttaa
     1681 gtttatcagt tcttcctgaa aagtgattct ctccagttga gtctgtaatc agttctcatt
     1741 tcgttcttca agtaaacaga gcaatcatat gaaacatcaa agagatcatt aatagttgcc
     1801 ggatttcttg tccctctaag ccttttcgta tgcttcggta atcacgtttt catcagcatc
     1861 attgaagatc atcagaccgg gacttacgag actccaggat cttcaatatt ttcgtccaat
     1921 tcgcaatcct tatggaaagg gatattgaag tcgagaaaaa cccggtagca gtggcgaaag
     1981 aggggtaaac actgcgtgct ttcacatcta atccatatct tctatcagaa cgagatctct
     2041 ccactttcgt tgcttttccc accatcggtc gaataaaatt ctagtatgaa gaatccagcg
     2101 ctcaaagttg cgttactata cgttgtattt ggtgttatat ggatcttgtt ttctgatatg
     2161 atcgtcgatt tgatgtttgt cgaaaaagat cttgttgctc atgctcaaac ctacaagggc
     2221 tgggcctttg ttatcttcag tgggattctc ttctactttc tcatttaccg tgagttcagc
     2281 gagaaaaaca agactcaact cgaactcgtt aagcagaaag acctttccga cgcagtcctc
     2341 gacacggtgg gcgtttttgt tgcagtatta gatagcgaag gggtcattgt cttcacaaat
     2401 gagacttttg aggagattct ttcactcaaa tcggaggata taattggaaa gaactgctcc
     2461 gaagtcttcg catcccctga tcttgcaaac tggatcgaga acaccgcaag caagacactg
     2521 gatagagaga tagagaactt ctatgaggcc gatttagaaa cctcgtcgac tactctacac
     2581 atccgatggg cattgagcaa tcttacaagc tggagagggg aacatgatta cttcgttttg
     2641 acaggtgtag atataactca actggtagaa tcagaaagaa gcgcaacgca tagattggca
     2701 aatattcgcg cactgcacga aatagatatg gccgtcagct atcacctcga acttgaaaag
     2761 atgctggatg tctttctcga aagattgatc tcgaggcttg gcgtagatgg cgccgatgtc
     2821 ttcttaattg acgaagaaag aagtgttctg agattcgctc atggaaaggg aatagtcact
     2881 ggagaaatgg acttcagaga gcttccaatg gaaggaacta tacctagttc ggttgcttcg
     2941 tcgggaaagc cttactccgg tccgttggac acctctagca atcccgaatg cccgaggctc
     3001 aagaacctga tagatatgaa agtgaaggac tatcatgccg taccccttga aacgagagga
     3061 aaaattctgg gagtccttga aacattcgac atgatgcaaa ttcacagaga cagtgaatgg
     3121 aatgattttc tgcagatgct tgcggcacaa gcttcactag cgatagatgt tgctctgatg
     3181 atagataatt tgaaagacag caacaggaag atcgcagaag cttacgatca gactttggaa
     3241 gttcttgtag gaacactgga aatgagagac atgaacacca agaaacactc aaggagagta
     3301 gccgatctta cagtttggct tgccgagaaa atgggggcat cggaagaaga aatagaaaac
     3361 atgtacagag gcgccctgct gcacgacatt gggaagattt ctattccgga cagcgtgtta
     3421 ctgaaggaag gacctctatc tgaagacgaa tggaaggtaa tgaaaactca tccgacaacg
     3481 gcgtatgaag tgttgtccca agtcgagtac cttagacctt cgctggatat tccttactgt
     3541 caccacgagc gttgggacgg aagcggatat ccccgcggac ttaaaggaga agagattcct
     3601 ctggctgcaa ggatatttgc cgttgtagac gtttatgacg ctctgacttc agataggccg
     3661 tatagaaaag cctggagcaa agagaaaacc gtagagtacc ttctagagaa ctccggaaag
     3721 ctattcgatc cagccgtgat agaaaagttt cttgagatat tgagagtcag ttcaccatca
     3781 taagtcgacg caaaaacaat tattcatcat ttctcgcatg agcctccgca attacatctg
     3841 agaactgctt gcgcaatagt atcccattca agcgttatag ttatttccgg gacctcatag
     3901 gtcgttccat caacgagcca ttcttcaaaa tgatatccgg aatcaggcgt cgcgcttaga
     3961 tcaactgagg atcctttatc gtaaacgtag gttccttcgg gaggactgac ggtccctctt
     4021 ccatatttgt agatctcaag agttactttc tctgcttttt gcacaaactc tgccgagact
     4081 gatttgtcac ggtccatgat aatcgaagta gtttcgctat ctgaataaaa agtaccgtca
     4141 aggtaccagg cctcgaactc ccatccgtct gcgggagtag ccgaaagggg cacggaggtt
     4201 ccctctatgt atgtgtgatc tccgaccgga ggactgaccg aaccctgacc gattggctca
     4261 accattgtca gggtgaaaac tgttggcggt tcttcaacaa aaaacgcctt agctgtcttg
     4321 ctggcattca tggtgatcgt tatctgcgaa ttgtatgaga agagagctcc atctatctgc
     4381 cagaaatcaa agtcccagcc gttggaaggt gtagccacaa gggtaacaac tgaaccctct
     4441 ttatatatac ttataccgac cgcaggttcg acatttccgc ttccggaggg ctcaagcatt
     4501 gtaagcgtgt gtgtcttacg gacaaagact gccttaacat ttctatcggt gttcattgtg
     4561 agttcggctt catgttcaga ggaatagact gcatcttcaa caagccagtg ttcaaagagc
     4621 cacccttctg ccggtgtcgc ttgaagtgtt aaagactttc ctgcatcata cttgtgatct
     4681 ccgacag
//
LOCUS       DAOQYI010000093         4501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_19650_length_4501_cov_52.158570, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000093 DAOQYI010000000
VERSION     DAOQYI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19650_length_4501_cov_52.158570"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PKL93_10860"
     CDS             <1..1191
                     /locus_tag="PKL93_10860"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNQ71799.1"
                     /translation="NIAISSRVETGSRLMKWKETEEESMKTMDKIGVSLDPRREVSEL
                     SIAERQIVAICRAINAKARLVIMDEPTASLSKKEVQSLIRVINELQNREISTIFVSHK
                     LDEIMEVAQRVTVLRDGKKVGAFDASELTRQRLSFLMTGKEFEYSKLPPYSGDEVVLE
                     VRKLSRRNNYKEIDLKVHKGEIVSITGLMGSGRTEFVLSLFGMNPPDEGEIFVEGKRV
                     SPGSAVDAMRAGIAYVPEDRLQHGLVMEQPVSKNIVLTVLKRILSRIRLLSKVKERTE
                     VRRWIDELSIKIPSIDSPVNTLSGGNQQRVVIAKWLAINPKVLILDSPTVGIDVAAKD
                     SIYKIIRELAAEGISIIMITDEAEEAIYHSNTTYIMSAGRIIGKYNSIELTEKELYEK
                     INGQ"
     gene            1169..2176
                     /locus_tag="PKL93_10865"
     CDS             1169..2176
                     /locus_tag="PKL93_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71800.1"
                     /translation="MRRSTDSNSGLRKAFGKSEFYLLLVIIVVSLFFTVMKPSFLTFT
                     NLYGMVESNSFLAIMAAGVLVVLISGGIDISFTAIATVAQYIMATIVINWGGNMFLAF
                     AIGGVVGIALGLVNALLIYFLKTPPIIVTIATMNLFYGLLIFISGGTWIYGFPMWFME
                     RNLVRFGDSPGITIPILVLVLSFILTWLILKYTALGRNIYAVGGNQEAARRVGISVLK
                     TQLFVYCYMGFLAGIASTVQANMMLTVAPNALMGRELEVLAAVVLGGASLAGGTGSIL
                     GTVLGFGLIVIVQNGLTLLGISSYWHKVFVGAIIVISVSITAYQRKVRERKGAIINVE
                     D"
     gene            2163..3155
                     /locus_tag="PKL93_10870"
     CDS             2163..3155
                     /locus_tag="PKL93_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71801.1"
                     /translation="MLKTDTGRGRVSKHSEILSLGAILVILVLLFSLALPGKFLRPSN
                     LQSMAFQLPELGVLAFAMMITMLTGGINLSIISSANLSGIIMAMILTGDLASGPGGAG
                     LGWTIFLAVLAGLSISLLVGLVNGMIIAYIGVSPILATLGTMTFLEGISLVITRGYVI
                     SGLPRELLVIGNGTFLGVPVPMFILVAVAVGVAIILNKTRLGLSTYMIGSNIKATGFS
                     GINTNKVTILVYMISGLLAGIASLIMIARFNSAKAGYGSSYLLVTVLVSVLGGINPNG
                     GFGKVSGVFLGLILLQVISSGLNLLGISQFLTLALWGALLLGVEALRLTRRRVR"
     gene            3180..3920
                     /locus_tag="PKL93_10875"
     CDS             3180..3920
                     /locus_tag="PKL93_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNQ71802.1"
                     /translation="MALKKEDRRQNILSVLSERESINTQELASLMDVSLVTLRRDLRD
                     LQKSGLVINGYGVVRAVNQETDPNSFFLKRLRLAREAKERIAEKAIEFVEENDVLFID
                     ESTTCYVFALKLSRVFKKLHIITNGINILLALSKVQGFSVESSGGSLQYGFDSLIGPR
                     AESLVGSIYANKFFFSCASLRKKIGTFELSPFSASIKRKMLLNSSTKILLVDNSKFDV
                     IAPFKMAEIDEIDRVITEDRDFLPSEVI"
     gene            3990..4367
                     /locus_tag="PKL93_10880"
     CDS             3990..4367
                     /locus_tag="PKL93_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71803.1"
                     /translation="MPTPSGSDAPKSISGSDAAAQEVMPGVSSGEVRPLSAGSIQEPV
                     KRRESSPPVKASPLPVPLRGASFSFKLRPVNASSGKNNRKTQLMQNIRTVALRASSGS
                     REKSRCALKRKNPLVAERKNSSF"
BASE COUNT         1224 a          965 c         1151 g         1161 t
ORIGIN      
        1 aacatagcta tttcttcgag ggtggaaacc ggaagtcgtc tgatgaaatg gaaagagacc
       61 gaagaagagt caatgaagac gatggacaaa attggggttt cactagatcc cagacgcgaa
      121 gtcagcgagc tatctatagc ggaaaggcaa attgtggcga tatgcagggc tataaatgcc
      181 aaggcgaggc ttgtaataat ggacgaacct acggcttctc tcagcaagaa agaggttcag
      241 tcgctaatta gagttataaa tgaactgcag aacagagaaa tctcgacgat cttcgtcagc
      301 cataagctcg atgagataat ggaggtagcc caacgggtaa ccgttcttcg tgacgggaaa
      361 aaagtcggtg ccttcgatgc ttccgaactg acgagacaga gactctcatt tctcatgaca
      421 ggcaaggagt tcgagtactc caaattgcct ccgtattctg gcgacgaagt tgttctagaa
      481 gtcagaaagc tctcaaggag aaataattac aaggaaattg atttgaaggt ccacaagggc
      541 gaaatagtaa gtatcacagg actgatgggt tcaggcagaa ccgagtttgt cctttcactc
      601 ttcggaatga atcctcccga cgaaggagag atcttcgtag aagggaagag agttagccct
      661 ggaagcgccg tggacgctat gagagctgga attgcgtatg tgcctgagga tcgactgcaa
      721 cacggactcg ttatggaaca gcctgtaagc aagaatattg tgctgacggt tctaaagaga
      781 atactttcga gaatcaggct cttgagtaaa gtcaaagaaa gaactgaagt gagacgttgg
      841 atcgacgaac tctcaatcaa aattccctcg attgactctc cggttaacac gctttccggc
      901 ggcaatcaac agagggttgt aattgcaaag tggttggcaa taaatccaaa agtattgatt
      961 ctcgactccc caacagtggg aatagacgtt gccgcaaagg acagtatata caagataatc
     1021 agagagctag ccgcagaagg tatttcgata atcatgatca cggatgaagc cgaagaagca
     1081 atctatcatt caaacacgac ttatattatg agtgcgggaa gaataattgg aaagtacaac
     1141 tccatcgagc tcacggagaa agagctgtat gagaagatca acggacagta attcgggatt
     1201 gagaaaagca ttcggcaaga gcgagttcta tctcttgctt gtaataatag tcgtctcact
     1261 cttcttcacc gttatgaagc caagttttct tactttcacc aatctctacg gaatggttga
     1321 gagcaactcc tttctggcga taatggccgc aggtgtgctg gttgttttga tttcaggagg
     1381 aatagatata tcatttacgg caattgctac cgttgctcag tatatcatgg ctactatcgt
     1441 tatcaattgg ggcgggaata tgttccttgc ctttgctata ggaggggttg tcggaattgc
     1501 tctgggtttg gtcaacgctt tactgattta tttcctcaaa actccgccaa taatcgtgac
     1561 gatcgcaacc atgaacttgt tctatggcct tctaatattt atcagcggcg gtacctggat
     1621 ttacggcttt cccatgtggt ttatggagag gaatctagtt agatttggag acagcccggg
     1681 aattactata ccgattctag ttcttgttct ttccttcatt cttacttggc tgatactaaa
     1741 gtacactgct ctgggcagaa atatctacgc agtgggcgga aatcaggaag ccgcaagacg
     1801 tgtgggaata agcgttctca agacgcagct atttgtctat tgctacatgg gctttctggc
     1861 aggcatagcc tcaaccgttc aggcgaacat gatgttgact gtcgccccca atgccttgat
     1921 gggcagggaa ctggaagttc tggcggctgt tgttcttgga ggggccagcc ttgccggagg
     1981 tacgggcagc attctaggga ctgttcttgg ctttggtttg attgtcatcg tacaaaatgg
     2041 ccttactttg cttgggattt cctcttactg gcataaggtt tttgtgggag cgatcatagt
     2101 catcagcgtg agtataacgg cctatcagag aaaagtccgc gagagaaaag gagcgataat
     2161 caatgttgaa gactgacaca ggcaggggaa gagtatcaaa gcactccgag attctctcgc
     2221 ttggagcgat cttggtcatc ctggtactat tgttctcgct cgctcttcct gggaaatttc
     2281 taaggcccag caatctccaa tcgatggcct tccagcttcc cgagctcgga gtccttgctt
     2341 tcgcgatgat gataacaatg cttacgggtg ggattaacct ttcaataata agctcggcaa
     2401 atctctctgg gataatcatg gcgatgattt tgaccggtga tttagcatct ggtccgggag
     2461 gggccggact gggctggacg atctttctgg ccgtactcgc ggggttgtcg atttcactcc
     2521 tagtgggact cgtaaatgga atgataatcg catacatcgg cgtatctccg atactcgcga
     2581 cgctcggtac tatgaccttt ctcgaaggaa taagcctggt tataaccaga gggtatgtca
     2641 tttcaggcct accgagggaa ttacttgtga ttggcaatgg aaccttcctg ggagtaccgg
     2701 taccgatgtt cattctagta gccgtcgcgg tgggtgtcgc gataattcta aacaagacgc
     2761 ggttgggttt gtcgacctac atgatcggat ccaacatcaa agctaccggt ttctctggaa
     2821 taaataccaa caaggtaacc attctcgttt atatgatttc gggccttctg gccggtatag
     2881 cctctttgat aatgattgcg agattcaatt ccgcaaaggc cggttatggt tcttcgtatc
     2941 ttctggttac cgtactggtt tcagttctag gtgggatcaa tccaaatggt ggcttcggga
     3001 aggtgtccgg tgttttcctt gggttaattt tgctgcaggt tatctcaagc ggtttgaatc
     3061 ttcttgggat aagtcagttt ctcacacttg cgctttgggg tgcactcctc ctaggtgtag
     3121 aagctctaag attaaccagg aggagagtca gataagacag atatgctgga gttgagcaca
     3181 tggctttgaa gaaggaagac agaagacaaa acatacttag cgttctttcc gagagagaat
     3241 cgatcaatac tcaagaactt gcgtctctaa tggatgtatc tctcgtcacg ctgagaagag
     3301 acttgagaga tctgcagaaa tccggccttg ttatcaacgg atacggagtc gtaagagcgg
     3361 tgaatcagga aacagatcca aacagctttt tcttgaaaag gctgaggtta gccagagagg
     3421 caaaagagag aatcgctgag aaggcaatcg agtttgtaga agagaacgat gtccttttca
     3481 tagatgaaag cactacatgt tacgttttcg ctttaaaact ctccagggtt ttcaagaagc
     3541 tgcacataat caccaacgga atcaacatac tgctcgcgct ttcaaaggtt caggggtttt
     3601 ctgtcgaatc tagtggcggc agtctacagt acggatttga ttcgctcatc ggccccaggg
     3661 cggagagtct tgtcggttca atctatgcaa acaagttctt cttttcatgc gcctcgctaa
     3721 gaaagaaaat aggaacattc gagttgagcc ccttttccgc cagtataaag agaaaaatgc
     3781 tgctcaattc cagcacaaag attcttcttg ttgacaacag caaattcgac gtaatcgctc
     3841 cgttcaaaat ggccgaaatc gacgaaatag acagggtcat cactgaagac agagacttcc
     3901 tgccctctga agttatatga gaaggctgct tcgcaggaag tgatgctttt tgattggacg
     3961 caaggcgccg aaaaacatag gcggacgcaa tgccaactcc gtcgggaagt gatgcgccaa
     4021 aaagcatcag cggaagtgat gctgctgcgc aggaagtgat gcccggagta tcatccgggg
     4081 aagtgaggcc gctttcagcg ggaagcattc aggagccagt caaaaggcga gaatcatcgc
     4141 cgccagtgaa ggcttcgcct ttgccagtac cacttcgtgg tgccagtttc agcttcaagc
     4201 tgaggccagt caatgcaagc agtggcaaga acaaccgaaa aacacaactg atgcagaaca
     4261 tcaggaccgt cgcacttcgt gccagttccg gttcgcggga gaagagccgc tgtgcgctta
     4321 agagaaaaaa cccgctggtc gctgagcgaa agaattcgtc attctgaact tgattcagaa
     4381 tctctcgttt tttcacttcg accgataacg aagaacaggt tttttattcg gtgaacggag
     4441 aaccattcaa cggccaacca gtatctttgc tctttcctac caccaacccg ctaacccaga
     4501 c
//
LOCUS       DAOQYI010000094         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_20605_length_4350_cov_85.154831, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000094 DAOQYI010000000
VERSION     DAOQYI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20605_length_4350_cov_85.154831"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4350
                     /locus_tag="PKL93_10885"
     CDS             <1..>4350
                     /locus_tag="PKL93_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNQ71804.1"
                     /translation="DLQFIVMDEVHSYVGELGSEVACLLRRLKLVVPNPENITFIGTS
                     ATVASRDEEPETVVKRFGSRLFGVEQEDIRLVTESYIELEREPDEYVPLFPRDPGEIL
                     KQMVFEVSQIPEEGEPGSEFYKLLTQLTYKTINRDNAYATLKKNVIISELQELFSKPT
                     LLEEAVERIKALSGRKDASKDALAAEIYSYLLAGLVVKEDNEPLLRPKIHFFARGIQN
                     LKVAFVDGQRQIILNDIDELQDHYYFSLYGCARCGQHYFVIPSASESQVEGVTLYTPV
                     GMGDEPEESRMVLLTDVVTGDEQAYPSQNVWVCEKCGTVHDSPVQKCMNTLCGSDRIV
                     QMKKVPTAARLRCLSCGVSLGKDDVDEERSSNLMPSVSNEALDILILAQNMLNSVSKD
                     AAKLLVFTDNRQEAAFQAAFINQRSKRFRFRDYLYSVLEEGRAYTSDELAGEIAERIR
                     EQGEYRDFEVENSMRPDYFKDSVEWFLLEDFFMPRYRRGSLENLGLVKLTYGNVVSSK
                     EFVNRWAEEFGAPVEEILAIIENLLDHMRVNTGISHTLLGVTNRDPRVARGYINLSKW
                     FGPEVYILNAPAYNAQSNLKWWISNNGTSYYQRFMKKAFPENPDLADDFLTELWHFLV
                     EERVLVERENSHFAINSEAIYFTKAPKREEDNLPHLYECRKCGTRTFRKTPGLVCPRH
                     NCDGKLTDVSVDTDDYNVKRHLSNREIRSLKAFEHSGQVRRPERERIEKEFKEENGTV
                     NCIVATPTLELGVDIGKLDMVLLRNAPPTPANYDQRAGRAGRRHRIAVVTTYCGRSSH
                     DLYFFSRPEEMITGKIKVPAFSMVNIPLIAKHCHSAITTKLHSTTSKDEEKALLEAYL
                     PNYINAFFNGINLFGGNAAETLKNLFGFSKPLEELINRHYDVLRDELERFFRYWPPEA
                     LKTLRESVEGELEKFLIDGTPQQLENILVHIRDDLIFLIEERNRLHADQFGAREHTAI
                     TNYLRSFSNPQGNEASLNDFYTISVLVKYGFFPGYSLARGHVTARCLDNEGFVEIDRN
                     YNIALREFAPLSRLYAVGKKYRPTRYNFYNADTKAGFRQKFIIIEDYIMPENPDEPAF
                     DNPAQLKLDSIHITDPKLTLEGRPGDSENRRVIMGYDIRGIVKGNHLGGVTAKTDGMT
                     TSFRRKDVVVLINVGSKRDMPNYGYGFYICPECGVIEENTPNGKDHFMRHLETVHNKH
                     IQGERLNEYRRGLHAEFVSDVMVVEPFKSFEEAANYMEAMKVGASIELDISTSEIDSF
                     INGLEENYHVTFFETVPGGSGYIEVLFQYRESVMKTARTLLDSCDCKSACYRCLLSFW
                     NQPYHSVLNRNKAIEVLDYLLTSGATTAVKVPGTRREKSSDGNPESPAEVKFLNIVKQ
                     FGLPDPHLQYQVTISNMTTIADFAYPDKKLLIYIDGYKYHTGQGQEKIDLDKRQELIL
                     KTSGYEVIRIGAKD"
BASE COUNT         1302 a          951 c         1057 g         1040 t
ORIGIN      
        1 gatttacagt tcatcgttat ggacgaagtg cacagctatg ttggagagct cggctcggag
       61 gttgcctgtc tgctgagaag actgaagctc gttgttccga acccagagaa cataaccttt
      121 attgggacct cagcaacagt cgcctcacgt gacgaagaac ctgaaactgt agtaaaacgc
      181 ttcggttcaa gactctttgg tgttgaacaa gaagatatta gactggttac cgaatcttac
      241 attgagctcg aaagagaacc ggatgaatac gttccgctgt tcccaaggga tcctggagaa
      301 atcctgaagc agatggtatt tgaagtttct cagattcctg aagaaggaga accagggtca
      361 gagttctaca aactgctgac gcagttgacc tataagacca taaacagaga caacgcttat
      421 gcgacactga aaaagaacgt tattataagc gaattgcagg agcttttcag caagcccacg
      481 cttctggaag aggctgtcga gagaatcaag gcattatccg gaagaaaaga tgctagcaag
      541 gatgctctcg cggcggagat atactcctat ctacttgccg gcctcgttgt aaaagaagac
      601 aacgaacctt tacttcgccc gaagatccac ttcttcgcaa ggggcataca aaacctgaag
      661 gtcgcatttg tagatgggca gagacagata attctgaacg atatagatga gcttcaggac
      721 cactattatt ttagcctcta tggatgtgcc agatgtggcc aacattactt cgttatccct
      781 tcggcatctg aaagtcaggt tgaaggagtc actttataca cgcccgttgg tatgggtgac
      841 gagcctgaag aaagccgaat ggttttacta acagatgttg ttactggaga cgaacaggcc
      901 taccccagtc aaaacgtatg ggtgtgtgag aaatgcggta ccgtccacga ctcacctgtg
      961 cagaaatgta tgaacactct ttgtggttca gacagaattg ttcaaatgaa gaaggtgccg
     1021 actgcggcaa ggcttagatg cctctcctgt ggtgtttcgc tcgggaaaga tgatgttgat
     1081 gaggagagat cctcgaacct aatgccatct gtgtcaaatg aagctttaga tattctcatt
     1141 ctcgcccaga atatgctcaa ttccgtttcc aaagatgcgg cgaagcttct ggtattcaca
     1201 gacaatagac aggaagccgc cttccaggca gccttcataa accagagatc gaagaggttt
     1261 agattcagag actatcttta ctctgttcta gaagagggtc gagcatacac gagtgatgaa
     1321 ctcgcaggag aaatagctga acgcattcgc gagcagggtg aatacagaga ctttgaagtt
     1381 gaaaattcaa tgaggccgga ttacttcaaa gattctgtag agtggtttct tctggaagat
     1441 ttctttatgc cccgctatag acgcggaagc ctcgagaatc tgggactcgt taaacttact
     1501 tacgggaatg tagttagcag caaggaattc gtgaatcgct gggcagaaga attcggagct
     1561 cctgtggaag aaatactggc aataattgaa aatcttctag accatatgag agtcaacaca
     1621 ggtataagcc acacccttct cggagtaaca aacagggacc cccgagtcgc cagaggttat
     1681 atcaacctta gcaagtggtt cggccccgaa gtttacatac tgaacgcacc tgcctacaat
     1741 gctcagagca atctcaaatg gtggatttca aataatggaa cttcgtatta tcaaaggttt
     1801 atgaagaagg catttccgga gaacccggat cttgcagacg atttcctaac ggagctgtgg
     1861 cactttctgg tagaagagag agtcctcgtt gagcgcgaga actcccattt tgcaataaac
     1921 agtgaggcaa tttacttcac taaggcgcct aagagagaag aagacaatct tccccatctt
     1981 tacgagtgca gaaagtgcgg aacgagaaca ttcagaaaga cccctggctt ggtttgcccc
     2041 agacacaact gtgatggtaa attaactgat gtatcggtag atacagacga ttacaacgtc
     2101 aagagacact tatccaacag agaaatcaga agtctgaagg cctttgaaca ttccggtcaa
     2161 gtccgtagac ctgaaaggga gagaatcgag aaggaattca aagaggagaa tgggacggta
     2221 aactgcattg tggccactcc cacccttgaa ctcggtgtgg atataggtaa actggatatg
     2281 gttcttctta ggaatgcacc gccaactccg gcaaactatg atcagcgagc cggaagagcg
     2341 ggaaggcggc acaggatagc cgtcgttact acttactgcg gacgaagctc ccacgatcta
     2401 tacttcttct cgagaccgga agagatgatt accggcaaga taaaggtccc ggccttctct
     2461 atggtgaata tcccgctaat tgctaagcac tgccactctg ctataaccac aaaattacac
     2521 agcaccactt caaaagacga agaaaaagct ctgctggagg cctatcttcc aaattacata
     2581 aatgccttct tcaacgggat aaacctgttt ggtggtaatg cagctgaaac gctcaagaac
     2641 ttattcggtt tctctaaacc cctggaagag ctgataaata gacattacga cgttctcaga
     2701 gatgagttag agcgtttctt taggtactgg ccaccagaag ctctaaagac attgagggag
     2761 tcggtggagg gagagcttga gaaattcctc atagatggca ctccacagca gctggaaaat
     2821 attcttgtcc acattcgcga cgatcttatc ttcctcattg aagaaagaaa cagactgcac
     2881 gccgatcagt ttggtgctcg agagcacacc gctattacaa attacctaag aagtttttcg
     2941 aatccacagg gcaatgaggc ttcactcaac gatttctata ctatctctgt cctggttaaa
     3001 tatggcttct tccccggtta ttcactcgca aggggtcacg ttactgcccg ctgcctggac
     3061 aacgaaggct tcgtcgaaat agacagaaac tacaatatcg ctctcaggga gtttgcaccg
     3121 ctttccaggc tttacgcagt ggggaaaaag tacaggccaa cccggtacaa tttctataat
     3181 gccgatacta aggcgggctt taggcagaaa ttcatcataa tcgaagacta cataatgcct
     3241 gaaaaccctg atgaacctgc tttcgacaac cccgctcagt tgaagcttga ttcaatacac
     3301 atcacagatc ctaagttgac tctcgaagga aggcccggcg atagtgagaa tcgtagggtc
     3361 attatgggct acgacattag gggaatcgta aagggaaacc acctaggagg agtcactgcc
     3421 aagactgacg gaatgactac ttcgttcagg agaaaggacg tagttgtcct cattaatgtt
     3481 ggttcgaagc gtgatatgcc caactatggg tatggtttct atatttgccc ggaatgtgga
     3541 gtgatagaag agaatactcc taatgggaaa gatcacttta tgagacattt ggaaaccgtc
     3601 cataacaaac atattcaagg agaaagactc aacgagtata ggagaggcct tcacgcagaa
     3661 ttcgtgtctg acgtaatggt tgtggaacct ttcaagagct ttgaagaggc agctaattat
     3721 atggaagcga tgaaggtggg agcatcgatc gagctagata ttagtaccag tgaaattgat
     3781 agcttcataa acgggcttga agagaactac cacgttactt tcttcgaaac agtaccgggt
     3841 ggctcggggt acattgaggt gttgttccag tacagagaaa gcgtgatgaa gactgccagg
     3901 acacttcttg attcctgcga ctgcaagagt gcctgttaca gatgcttgct gagcttctgg
     3961 aatcaaccat accattcagt tctcaatagg aataaagcaa tcgaagtact tgactaccta
     4021 ctaacttctg gagctaccac agcggtgaaa gtaccgggca ccaggcgaga gaagagttcg
     4081 gatgggaatc cagagtcgcc ggccgaagtg aagttcctta acattgtgaa gcagttcggg
     4141 ctcccagatc ctcatcttca ataccaggta accatctcaa atatgacaac catcgcagat
     4201 ttcgcctatc ccgataagaa actccttatc tacatagatg gctacaagta tcatacgggc
     4261 caagggcaag agaaaataga cctggataaa aggcaagagc taattctcaa gacgagtgga
     4321 tacgaagtta ttaggatcgg cgccaaagac
//
LOCUS       DAOQYI010000095         4208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_21521_length_4208_cov_69.327715, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000095 DAOQYI010000000
VERSION     DAOQYI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21521_length_4208_cov_69.327715"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKL93_10890"
     CDS             complement(<1..555)
                     /locus_tag="PKL93_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Two component regulator three Y domain protein"
                     /protein_id="HNQ71805.1"
                     /translation="MKKYILFVLSLSLIFLLVGCPAVNKSPEVVKLDGASGKVAADSW
                     TFLWSGSDADGSIIRYEFRKDFADWESNGNETGYTWSEYSEGEHTFEVRALDNEGAYS
                     EIIVWTFNYDPPNVPPAVTKTGGLEGETEESSNTFFWTGNDPDGEIVRYEIRKDSGEW
                     VDAELSTEYTWNGYSEGEHTFEVRA"
     gene            complement(761..1615)
                     /locus_tag="PKL93_10895"
     CDS             complement(761..1615)
                     /locus_tag="PKL93_10895"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNQ71806.1"
                     /translation="MNRKRGIGKIVTGRAAVDGVGVKLVRVLGHRDVEDFDPFLLLDA
                     FDSTDPEDYAKGFPWHPHRGIETVTYLVSGIIEHGDSIGNKGEILDGDCQWMTAGSGI
                     IHQEMPKPAERMLGVQLWLNLPAKDKMTDPRYGDIRSENVPIVTEEGASIKVTAGSYK
                     ELAGAFEGRYVKATYLDVLLDAGKEWSYKSEDGSTLFIYILLGSGSLDPGQPEVIENK
                     RAVLFGEGEQFWVKAGTEGIRFLLLSGKPLKEPIAWGGPIVMNTNEELELAFRELRED
                     SFVKNDGG"
     gene            2549..2884
                     /locus_tag="PKL93_10900"
     CDS             2549..2884
                     /locus_tag="PKL93_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71807.1"
                     /translation="MKRLLVLLFVVLSTIVYCNDPYFTEENVNLFIEQLESEGFIVQQ
                     GTFYSFDMPDLFSNYITPSCYGNNADTPYCVYYMPPVPSQTAKNTFPFTFRLREDEAV
                     VFLGWTPQR"
     gene            2881..3246
                     /locus_tag="PKL93_10905"
     CDS             2881..3246
                     /locus_tag="PKL93_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71808.1"
                     /translation="MKYFSNETLLMFRYLPNVEGPVRIFGGVGDTVNITNVKAGDSIL
                     EKTAGTVYNAPLIIITTPDRNIDRIIRETAREVGFTEDIMTTDVVHSVLLNLGIEEDC
                     DELIIGARFAFFNNPDDKA"
     gene            3286..4035
                     /locus_tag="PKL93_10910"
     CDS             3286..4035
                     /locus_tag="PKL93_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71809.1"
                     /translation="MEPPYSSNRRGWVLRLTQSDPIELDPYPVPNLTPRDTGDYSELN
                     LKSTMDSLEEAIIDHYSDLNADVLRTYRWVDISYHAIQNNTDVLGDSRDAIYLKSDPF
                     ALEEDPDDFAIVFGPIHSLTGKAIYSSFVVYSNDLVKDLLPYESRILYGFLSINSERS
                     AETKLGLPGSAERFLPDDPNAKYFYVWKVSKSNPDNEDYCLLVPEPTLKRITYDELMI
                     GFRAYVNPTTGVGPSYEEILMDKVIHFRPKK"
BASE COUNT         1147 a         1013 c          895 g         1153 t
ORIGIN      
        1 agcccttact tcaaatgtat gctcgccttc ggaataaccg ttccatgtgt attcagttga
       61 aagctctgca tcgacccatt ctccagagtc ttttctaatt tcgtatcgca ctatttcacc
      121 atctggatcg tttccggtcc aaaagaatgt attactcgac tcttccgttt ctccttcaag
      181 gccaccggtc tttgtcactg ctggtggcac attgggaggg tcgtaattga aggtccagac
      241 gattatctcc gaataggctc cttcattgtc gagcgccctt acttcgaaag tgtgttcgcc
      301 ttcggaatat tcgctccatg tatacccggt ttcgtttccg ttgctctccc agtcagcgaa
      361 gtcttttctg aactcatatc ttatgattga accgtcggcg tcacttcccg accataagaa
      421 tgtccagctg tctgcagcta ctttgccgct agctccatcg agcttcacaa cttcgggcga
      481 tttgttcact gctggacaac caaccagcag gaaaatcaaa gacaaagaca gaacgaaaag
      541 gatatatttc ttcaagtttc tctcccccta ctaaatgctc caaacagagc tggctaaata
      601 acaagtctgc acattcaaga aacaggtgag gtaaatccaa tcgttaatta cataacatga
      661 cgatgcaata cgaaagaacc gtcagaacga cttctatgag tagtttatca tctgaagcat
      721 tcaacgggtc ttctcgaaat gtcatttcag ctaccggtag ttatccgccg tcgttcttga
      781 cgaaggaatc ctcgcgtagc tccctgaaag caagttcaag ttcctcattt gtgttcataa
      841 ctatggggcc tccccatgca atgggctctt tcaaaggctt cccagacaaa agaagaaacc
      901 taattccctc tgtccctgct ttgacccaaa attgctcacc ctcaccaaag agaactgctc
      961 gcttgttctc gatgacttcg ggttggccag gatcaagact gccactgcca agcagaatgt
     1021 agatgaacag agtggatcca tcctcagact tgtatgacca ttccttcccc gcatcgagca
     1081 acacgtcaag atatgtggct ttcacgtacc tgccttcaaa ggctcctgcc aattccttgt
     1141 aagacccggc ggttactttt atacttgcgc cttcttcagt tacaatcgga acgttttcac
     1201 ttcttatatc accgtatctc gggtcagtca tcttatcctt tgccggaaga ttcaaccaca
     1261 actgaacccc aagcatcctc tctgctggct tgggcatctc ctgatggata attcccgaac
     1321 ccgcggtcat ccactgacag tcaccatcta gtatctctcc tttgttacca atgctgtcac
     1381 cgtgttcgat aatcccgcta acaagataag ttacggtctc gataccccta tgaggatgcc
     1441 aagggaaacc ctttgcatag tcctccggat cagtggaatc aaaggcatcc agcagaagaa
     1501 agggatcaaa gtcctcaacg tctctatgac ccaaaaccct aacgagtttc accccgaccc
     1561 catcaacggc agctctcccc gtcacgatct tgccaattcc ccgttttcta ttcattggca
     1621 ttctcctctt tacttcgcaa cggattctca tgcatcaata ctcagaagcc tgtcacaaaa
     1681 atggtatctg aagatcccca gtcataaatg acttccatct gaaccccatg atctattatc
     1741 ctagataagc ctgcaagaat atccttgttt atcagatcaa agacagtagg aaggccggac
     1801 cttgtttggc ccggctttaa gggatggggt gagaaaggta atgaaggttt gctctgtcag
     1861 ttctgctgcg cctggtaatt agtcgttgct aactttactt ctatgtttca tactcgcctt
     1921 agagttcttc agttcatctt aaaacagatt ataagaaagg attttgggag tattgtcgca
     1981 gaagtatggg aaaagatgag agataacctg aaagttgtct tcatttctcg gcaaacctcc
     2041 ttgaaacagc atggcatgcc cagaaaggga agattgagaa atctgcagat cactgagtgc
     2101 ataatgttcg atctctttgg attcacagga gatgatgata gaattccggt gtgcactaaa
     2161 tatctaggat caattttcca aggaagatga accggtcgga aaggtgtcgt tgccgggggt
     2221 catagacacc atccggatgc aatgaatatg aattagtatt cggcaacagt cgctatttgt
     2281 gaatcccctt ctagatgccc gttatccttt catactttta caaaatcgta tacacctacc
     2341 tgtcaatact tacagagaaa ttgttcgaag ttaatgcgaa tttccctttg ctttgcagtt
     2401 ttctctttgt tttctaattg gcgacgtatt tttggatact tatctgaaag catattctga
     2461 tcttgaaaag aacagttttc ttcagccgag tttacagaga tattttgaaa tcgtctgata
     2521 atcttgaaaa cttcaaggag gtatgaatat gaaacgtctg ttggtccttc ttttcgtggt
     2581 gctttcgacc atagtctatt gcaacgaccc ctacttcacc gaggagaatg ttaacctgtt
     2641 tattgaacag ctcgagtcgg aagggttcat cgtgcagcaa ggtacatttt actcattcga
     2701 tatgcccgat ctcttctcaa attacattac gcccagctgt tacggaaaca atgccgatac
     2761 accttactgt gtttactata tgccgccggt tccctctcaa actgccaaga ataccttccc
     2821 tttcactttc agacttaggg aagacgaagc tgtggttttt ctgggatgga cgccccagag
     2881 gtgaagtact tcagtaacga aacacttctg atgtttcgat acctgccaaa tgtcgaagga
     2941 ccagtaagga tattcggagg tgtgggagac acggtgaaca taacaaacgt aaaggcagga
     3001 gattccattc ttgagaagac agccggcact gtgtacaatg cccctttgat aataattaca
     3061 acccccgaca gaaatatcga caggattatc agagagactg ccagagaagt cggttttacc
     3121 gaggacataa tgaccacaga cgttgtgcat tcggttctgc tgaatctagg aatcgaagag
     3181 gactgtgacg aactcataat aggcgcacgt ttcgcattct tcaataatcc ggacgacaaa
     3241 gcctaagcat tggatccatc cgctctcgaa gacgggaaat tctggatgga gcccccctac
     3301 agttcaaacc gtagaggatg ggtcttacgt ttaacacagt cggatccaat tgaacttgac
     3361 ccctaccctg ttccgaatct gacccctaga gatacgggag attattcgga gttaaaccta
     3421 aaatcgacaa tggacagtct tgaagaggca attatcgatc actactcgga tctgaacgca
     3481 gacgttttgc gaacttacag gtgggtagat ataagttacc atgcgatcca gaacaatacc
     3541 gacgtcctgg gagacagcag agatgccatc tatctcaaaa gtgatccatt tgccctcgaa
     3601 gaagacccgg atgatttcgc tatagtattc gggcctattc actctcttac gggaaaggct
     3661 atttactcga gttttgttgt ctattcaaat gatcttgtaa aggacctgct tccttacgaa
     3721 tcacgtatcc tttatggatt cctcagcatt aacagcgaaa ggagtgccga aaccaagctc
     3781 ggattgccgg gaagtgcgga gagatttctt cctgacgatc caaatgcaaa gtatttctac
     3841 gtttggaaag tatccaagag caatcccgat aatgaagact actgcctctt agtaccggaa
     3901 ccgacattga aaaggatcac atatgacgaa ttgatgatag gcttccgtgc ttatgtaaat
     3961 cccacaaccg gtgtggggcc atcttatgaa gagatactga tggataaagt aatacatttc
     4021 cgtccaaaaa agtagtccgt caatgacacg tggtcagggg taggaggttt ggggttggtc
     4081 aggagcaaac aattagttgc aagttccagt gtgccaagaa ggcgaagaag gaaacttcta
     4141 gccatgctta attgaagatg agggagggcc cctcgaagcc gtcatgcagg tggaggcttc
     4201 aaaccgcc
//
LOCUS       DAOQYI010000096         4134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_22034_length_4134_cov_66.242952, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000096 DAOQYI010000000
VERSION     DAOQYI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22034_length_4134_cov_66.242952"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PKL93_10915"
     CDS             complement(<1..1370)
                     /locus_tag="PKL93_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71810.1"
                     /translation="MSKDSREHGRGHLGRQGGPVGPGMMRGGEKAKDLRGTLKKLVQY
                     LRKYQVLIIVVLCFAAAGALFSIMGPKILGTVTTEVFEGIMAKITGTGEGINFESIAR
                     TMFILLGLYVLSALFMYIQGWIMSGISAKVTYKFRQEISQKINRLPLKYFDKSSQGDV
                     LSRVTNDVDTISRTLNQSLSQIITSVTTVVGVTIMMFSISWQLTLIALLMLPVSMGLI
                     AFVVRLSQRYFKKQQQYLGKVNGHVEEMYGGHVVVKAFNGEAKSIQTFDKLNAELYDS
                     AWKSQFLSGMMMPIMTFVGNLGYVLITIVGGWFAVRKMITVGDIQAFIQYVRSFTQPI
                     AQIANIMNVFQQTAAAAERVFEFLEEEEEVAETTDPVRGVDHHGHVEFKNVRFGYDPE
                     KIIIKDFSCEVDKGHNIAIVGPTGAGKTTIVKLLMRFYDVNSGEILLDGHNIKDYTRH
                     DLRENFG"
     gene            complement(1367..3592)
                     /locus_tag="PKL93_10920"
     CDS             complement(1367..3592)
                     /locus_tag="PKL93_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71811.1"
                     /translation="MLKLIRYLKPYTVFIIVAVALLFVQAMAELALPDYMSNIVNVGI
                     QQGGIEDAVPEAISKEAFDNVSLFMSGEDKQKALSHYDLITKDSATYEENLKKYPALE
                     SKDVYVLKPGEIEDRQTLSLIFGKALMAYSGVKNGMTGSSGSFAPPEGFKIPEGANVF
                     LLMRLMPEAQRLEMLNQIDSMVEVMGENIVSQSAALVVKEIYEELGVDTTKLQSAYVL
                     STGLVMVLVTLLSTLSTIMVAFIASKIAAASARRMRRDVFEKVENFSNSEFARFSTAS
                     LITRTTNDITQIQLVIVLIIRMAFYAPIIGVGGVIRALEKSTSMSWIIALAVIIMLGL
                     IGIVFAIALPKFKKIQKLIDRLNLVTREHLSGLMVVRAFNAQEFEEERFDAVNKDLTR
                     TDLFVHRVMVVMMPAMMFVMNGTMLLIVWVGAHQIEASTMQVGDMMAYIQYTMLIIFA
                     FLMFTMMFIMIPRASVSAARISEILEVDPQIKDPKKPKKFGSDFKGTVEFKNVCYRFP
                     GAEKNILNDISFTALPGQTTAIIGSTGSGKSTLLNMIPRFYDVCGGQVLVDGIDIREV
                     NQHELRDKIGYVPQKGILFSGTIESNLRYANENASDEEIKRAAEIAQALEFIRAKEEG
                     FDSPISQGGTNVSGGQKQRLSIARALLKRPKILLFDDSFSALDFKTDAALRRKLKRET
                     GDSTMIIVGQRIATIKNADQILVLDEGNLVGIGKHHELMKSCQIYREIAQSQLSEAEL
                     A"
     gene            complement(3580..4053)
                     /locus_tag="PKL93_10925"
     CDS             complement(3580..4053)
                     /locus_tag="PKL93_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNQ71812.1"
                     /translation="MEESSRIQEMLTLVKRIMSVVKQNFESDFKKMGVTQSQILVMRV
                     LNHYGDMKVSDISKELDLSNSTVSGIIDRLVEKKIVQRKRSDEDRRIVMISLADEYRQ
                     PVKKGLNAFAQKMRKALSTMTEEDVDSIIRGLEKLEKILEESQEPRERGRSRCSN"
BASE COUNT          898 a         1082 c          969 g         1185 t
ORIGIN      
        1 ccgaagtttt ctctcaggtc gtgccgggtg tagtctttta tgttatgccc gtccagaagt
       61 atttctccgg agttgacatc gtagaagcgc atcaggagtt tgacgatggt cgtcttcccc
      121 gctcctgtgg ggccgacgat ggcaatgttg tggcccttgt caacttcgca ggagaaatct
      181 tttatgatta tcttttccgg gtcgtagcca aatctgacgt tcttgaactc gacgtggccg
      241 tgatgatcga ctcctctgac aggatcggtt gtctcggcaa cttcctcttc ttcttccagg
      301 aattcgaaga ctctctccgc tgcagctgcc gtctgttgga agacgttcat gatatttgcg
      361 atttgcgcga tgggctgagt gaacgacctc acgtactgta taaaggcctg aatgtcgccg
      421 actgtgatca tctttctgac ggcaaaccag ccgcccacta tggttatgag gacgtatcca
      481 aggttgccga cgaatgtcat tatgggcatc atcatgccgg aaagaaactg tgatttccag
      541 gcggagtcgt acagttcggc gttgagcttg tcgaaggttt gtatgctttt cgcctctcca
      601 ttgaaggcct tcacgactac gtgacctccg tacatctcct ccacgtggcc gtttaccttt
      661 ccgagatact gttgctgctt cttgaagtat ctctgggata gtctgacgac gaaagcgatg
      721 agccccatag aaacaggcag cattagcaag gctataagcg ttagctgcca gcttatcgag
      781 aacatcatta tggtgacgcc gacaactgtc gtgacggatg ttatgatctg gctcagactc
      841 tggtttagcg ttctacttat ggtgtcgaca tcgtttgtta cccttgacag gacgtctccc
      901 tgggaactct tgtcaaagta tttcagggga agcctgttta tcttctggga gatttcctgc
      961 ctgaatttgt atgtgacctt cgcggatatg cccgacatta tccatccctg gatgtacata
     1021 aacagggcgc tgagcacgta cagtccgaga agaataaaca ttgttctggc gatcgactca
     1081 aagtttattc cttcgccggt cccggtgatt ttggccatga ttccctcgaa tacttcagtc
     1141 gtcaccgtcc cgagtatctt cggccccatt atggagaaga gagcaccggc cgctgcaaaa
     1201 cagagcacta cgattatcaa aacctggtac tttctaagat attgaacgag tttcttcagt
     1261 gtccctcgaa gatctttggc cttctctcct cctcgcatca tgccgggccc cactggaccg
     1321 ccctgacgac ctagatggcc tcttccgtgt tctctactat ccttgctcat gctagttccg
     1381 cctctgaaag ctgtgattgc gcaatttctc tgtaaatctg acagcttttc atcagttcgt
     1441 ggtgtttgcc gattccaaca aggttgccct cgtcaagaac gagtatctgg tcggcgttct
     1501 ttatcgttgc aattctctgg ccaacaatta tcatcgtgct gtcccccgtt tcccttttga
     1561 gctttctcct cagcgctgcg tcagtcttga agtcgagggc cgagaagcta tcatcgaaga
     1621 ggagtatctt cggcctcttc agaagcgccc tggctatgga gagcctctgc ttctgccctc
     1681 cagacacgtt tgtcccgccc tgggaaattg gcgagtcaaa cccttcttcc ttcgccctta
     1741 tgaattcgag ggcctgggcg atctccgcag cccttttgat ttcttcatcg gaggcattct
     1801 cattggcgta tctgaggttc gattcaatgg ttccgctgaa gaggatgccc ttctggggga
     1861 cgtagcctat cttgtctcta agctcatgtt gatttacttc ccggatgtca attccgtcca
     1921 ccagcacctg accgccacag acatcgtaga acctcggtat catgttcaga agcgtagatt
     1981 tccccgagcc ggtcgagcct atgatcgccg tcgtctgtcc cggcagggcc gtgaagctta
     2041 tgtcgttcag aatgtttttc tcggcaccag ggaagcggta gcacacgttc ttgaattcga
     2101 cagttccctt gaaatcacta ccaaatttct tcggtttttt tgggtctttt atctgggggt
     2161 cgacctcgag gatctcggag attctggctg ctgaaacaga ggcccttggg atcattatga
     2221 acatcattgt aaacatgaga aaggcgaata tgatcaacat ggtgtactgg atataggcca
     2281 tcatatctcc gacctgcata gttgaggcct ctatctggtg cgcgcccacc cagacgatga
     2341 gcagcatagt accgttcatc acaaacatca tggccggcat catcactacc atcacccggt
     2401 gaacaaagag atcggttctt gtcaagtcct tgttcacggc gtcgaatctc tcttcttcaa
     2461 actcctgggc attgaaagcc ctgacgacca tgagaccgga aagatgttcc cttgtaacca
     2521 ggttcagcct gtctataagc ttttgtatct tcttgaattt cggcaaagct atcgcgaaca
     2581 cgattccaat caggccaagc attatgatga cggcaagtgc aatgatccag gacatggaag
     2641 tactcttctc aagagccctt atgactcctc cgacaccgat gatcggggcg tagaatgcca
     2701 ttctgattat gagaacaatc acgagctgga tctgggttat atcgtttgtt gttctagtga
     2761 tgagcgaagc cgtagaaaac ctggcaaatt cagagtttga gaagttctcg accttctcga
     2821 aaacgtctct tctcatcctt ctggccgagg cggctgctat cttcgaagcg atgaaggcaa
     2881 ccatgattgt ggacagtgtg ctcagcagag tcacgagaac catgaccaag cccgtgctga
     2941 gcacgtaggc gctctggagc ttagtagtgt ccacgccgag ctcttcgtag atttccttca
     3001 cgacgagcgc agccgactgg ctgacgatgt tttcacccat gacttcgacc atgctgtcga
     3061 tctgatttag catttcgagc ctctgagcct cgggcatcag cctcataagg aggaatacgt
     3121 ttgccccctc ggggatcttg aaaccctcag gaggcgcgaa gctaccgctt gacccagtca
     3181 tgccgttctt gactccggag taagccatga gggccttacc gaagatcaga ctgagggtct
     3241 gccggtcttc aatctcaccg ggttttagga catagacatc tttgctctcc agcgcagggt
     3301 acttcttgag gttttcttcg taagtggcgg agtcttttgt tatgagatcg tagtggctca
     3361 gagctttttg cttatcttct ccgctcatga acagcgagac gttatcaaaa gcctctttgc
     3421 taatggcctc cgggactgcg tcttcgatac cgccctgctg gattccgaca tttacgatat
     3481 tcgacatgta gtctggaagg gccaattccg ccatcgcctg aacgaagagc agggccactg
     3541 cgacaatgat gaagacagtg tagggtttaa ggtaccgaat cagtttgagc atcggcttcg
     3601 tcccctctct ctgggttctt gcgattcttc aagtatcttc tccagctttt cgagccctcg
     3661 aatgatggaa tcaacgtctt cttctgtcat ggttgacaat gcttttctca tcttttgcgc
     3721 aaaggcattg aggcctttct taactggttg gcggtactcg tccgcaagac ttatcatcac
     3781 gattcgccta tcttcgtcac ttctctttct ttggacgatc ttcttttcga cgagcctgtc
     3841 gattatccca gacaccgtgc tatttgaaag atcgagttct ttacttatgt cgcttacctt
     3901 catatcgcca taatggttca gcactctcat aacaaggatc tgtgattggg tcacgcccat
     3961 ctttttgaaa tcactttcga aattctgctt tacaaccgac attattctct tgacaagcgt
     4021 cagcatctct tgtattctag agctttcctc caacaaaacc accttctatg cacatcattc
     4081 gaactttcgc atacgaatac ttagacttcg aatatttcgc atacgaaata tatt
//
LOCUS       DAOQYI010000097         3946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_23483_length_3946_cov_55.423799, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000097 DAOQYI010000000
VERSION     DAOQYI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23483_length_3946_cov_55.423799"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="PKL93_10930"
     CDS             <1..1625
                     /locus_tag="PKL93_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71813.1"
                     /translation="DDTKEESSDDATVTIIVPKTVKLEAEVAAEFFWIREKIYEWEID
                     KKANPTQITFQVNEFEKDIEYTITATKTLKSEVDTHSYSGSVKVTNNGNSEAQNVKLT
                     ITLQYYDGSDWITIQTLPEVELGSISSGGDNTYGFGPIAFTPVDTNAQHRVKVEVSAD
                     APANTDEAYDDSFLSITGTTETNATAVLDDEFTYVPDGFAIVATDPDPLPFPMNLTDS
                     AVVQFSITIRKFSFGGSGTVTYEYPVTDAYISATVTGYMAGTYPGWCIQVNVPGIGGP
                     YTIQDVYNGGAPDSKMAKANYILNRYRDGDYPGVDYRHIQIAIWAIMEGSLNWNSWRK
                     MFDPNVPDGDKPLVQAIIDTAKEDFLPGCGDVVLMKALSANKQDVILEIIFPCEEEEF
                     RLKNTATLISDLQEIWDDAVVEIKLTLTDPETWIGDETAWGGNYEGDGASWWYYFDTN
                     GPATQHIYAGQNKVAGASVTYKDGKLTIVLGPNMRLQSGSETVKIQGYAEGKLPTERP
                     AGGQFTYKGTDLVVPVSPARYFAIHLDVEVLQ"
     gene            complement(1706..>3946)
                     /locus_tag="PKL93_10935"
     CDS             complement(1706..>3946)
                     /locus_tag="PKL93_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71814.1"
                     /translation="TAPQVEGFSFLNWTVNGETLNDNPLQININSPKKVIARYNAIPT
                     LEIPDQTVSTGTNLVLDLRNFASDSDGDPIVFSKVSGVGTISGNQFSYNATTAGTYSV
                     TVRASDGRGGEATDTFSITVESSNNAPLKPSNPAPSSGATGVGTSITLSWNCSDPDGD
                     ALVYDIYFGTTTNPARVSAGQTARSYTPSNLAEGTKYYWKVVARDSKGLETTGDLWNF
                     TTKSNQPPAKPSSPNPANNSSDIAVSITLSWNCSDPDGDSLKYDVYFGESSNPSKVAS
                     GITTKSYNPGNLQNGKDYYWKIVAIDAKGATTQGDVWKFRTVAAEIPPEDGIDMNGPV
                     YTGNVLLVSNESESANTTEYTGTLSSDYLPSGDILPKNLPIEAYAINPEIPLPDDIDL
                     SMKMRPDLVEAGFELAAVGSTRSFHVRNFKTNSNYQITATLQYIGETCEIWVENPSEI
                     TVSKATQLGQEFDNVIYPLVTEYFYTPSDVNGDGRVAILCFDIQDDFDNTGAYVGGYF
                     SSGDLFNTSTSNKMEIFYIDTYPTMHYPASNPVDVSRAYSTLVHEFQHMVNFNRNYFV
                     EKGSSMPTWLNEGLSMAAEHIYKGTLTSRISYYNYATSIKNGQSLLYWNDNGDVLANY
                     SLSYLFLQYVRTQGGGDPAIFRDILLDSCNDQRAVTAALAKRGINIGFGDLMTAFRIA
                     LLLKKTTGLYGFRDDSSFDGITVSYYTGGAKNLRGGSAVFKAINPSFTDPGNSGSSIQ
                     YVGICK"
BASE COUNT         1027 a          937 c          955 g         1027 t
ORIGIN      
        1 aggacgatac caaagaggaa agctccgacg atgctacggt aactatcata gtacccaaga
       61 cagtcaagct cgaggcagaa gttgcagctg aattcttctg gataagagag aaaatctacg
      121 agtgggaaat cgataagaaa gcgaatccta ctcagataac cttccaggtg aatgaattcg
      181 aaaaggacat tgaatacacc ataacggcaa caaaaactct caaatccgaa gtggacacgc
      241 actcttacag tggttcagta aaggttacca acaatggcaa cagcgaggct cagaacgtca
      301 agctgaccat tacacttcag tattacgacg ggtctgattg gataactatt cagactcttc
      361 cggaagtcga gcttggaagt atctcttctg gcggagacaa tacttatgga tttggaccga
      421 tagccttcac acctgttgac actaacgctc aacacagggt aaaggtcgaa gtctctgccg
      481 atgcgcctgc caataccgat gaagcgtacg atgattcttt cctgtcaatt acgggaacaa
      541 ccgagactaa tgccactgca gttctcgatg atgaattcac ttatgtacca gacggattcg
      601 cgatcgtcgc aacagatcca gatccacttc cattcccgat gaaccttact gattcggccg
      661 tagttcagtt ctccataacc ataagaaagt tctcatttgg aggaagtgga actgtcacct
      721 acgaatatcc agtaacagat gcatatatct cagccacagt caccggctac atggcaggta
      781 cgtatcccgg ttggtgtatc caggttaatg tacccggaat cggtggtccg tacacaatac
      841 aggatgttta taacggtggc gcgccagatt cgaagatggc aaaagccaac tacatactca
      901 acagatacag agacggtgat tatcctggag ttgactacag gcatattcag atcgcaatat
      961 gggcgataat ggaaggctct ctaaactgga attcatggag aaaaatgttt gatcctaacg
     1021 ttccagatgg agacaaacct ctcgtccagg ctattattga tactgctaaa gaagacttcc
     1081 ttccaggttg cggcgatgta gtcctcatga aggcactgag cgcaaacaag caggacgtaa
     1141 tacttgagat aatattcccg tgtgaagaag aggaattcag acttaagaat accgcgacac
     1201 tcatcagtga tctacaggag atatgggacg acgcagtagt tgaaataaag ctgactctta
     1261 ccgacccaga aacctggata ggcgatgaga ctgcctgggg cgggaactat gaaggagatg
     1321 gagcttcatg gtggtactac ttcgacacaa acggtcccgc cactcagcat atttacgctg
     1381 gtcagaataa agtcgccgga gcctcagtga cttataagga cggcaagctg acaatagtgc
     1441 tcggacctaa tatgcgcctg cagagtggaa gcgagactgt gaagattcag ggatatgccg
     1501 aaggtaaatt accgacagaa agaccagcag gagggcaatt tacatacaaa ggcactgatc
     1561 tggtcgttcc tgttagcccg gcaagatact ttgcgattca tctggatgta gaagtactac
     1621 aatagccata cagtaacttc tataactcaa aagccctcct gacggagggc ttttcatttc
     1681 tagaagacag ggattggtta ttcgatcact tgcatattcc gacatactgt atggaacttc
     1741 cagaattacc cggatccgta aagctcgggt ttattgcctt gaaaactgcg cttccacccc
     1801 tcagattctt cgcaccgccg gtgtagtatg agactgttat gccgtcgaaa ctgctatcgt
     1861 ctctgaagcc atacaatccc gtcgttttct tgagaaggag cgcaatccta aacgccgtca
     1921 tgagatctcc aaaccctata tttatccctc tctttgccag cgccgccgtc actgccctct
     1981 gatcgttaca actatccaga aggatatctc tgaagatagc ggggtctcct ccaccctgtg
     2041 ttctgacata ctgcaagaaa aggtaggata gcgagtagtt ggcgagaaca tcgccgttgt
     2101 cattccagta aagcagcgac tgtccgtttt ttatgctggt ggcgtaattg taatagctta
     2161 tcctgcttgt cagagtcccc ttgtagatat gttcggcagc catcgacaaa ccttcattca
     2221 accacgtcgg cattgaactg cccttctcca caaagtagtt tctgttgaag ttgaccatgt
     2281 gctggaactc gtgaaccaga gtgctgtaag ctctcgagac atctaccggg ttagatgccg
     2341 ggtagtgcat cgtcggatag gtgtctatat agaagatctc catcttgttt gaagtgctgg
     2401 tattgaagag atctccagag ctgaaataac cgcccacata tgctccggtg ttgtcgaaat
     2461 catcctgaat atcgaaacag aggattgcga ctcttccgtc tccgttcaca tctgatggag
     2521 tgtagaagta ttcagtaacc agaggataga tgacgttatc gaattcctga ccaagctgcg
     2581 tcgccttgga aacagttatt tccgaaggat tctcgaccca tatttcgcag gtctctccta
     2641 tatactgaag agtcgcggtt atctggtaat tcgaattcgt tttgaaattc cgaacgtgga
     2701 atgatctcgt gctgcctacg gctgcgagct cgaagcctgc ctcaactaga tccggtcgca
     2761 tcttcatcga tagatcaatg tcatcgggga gaggtatctc aggatttata gcatacgctt
     2821 caatcggtaa attcttggga agaatatctc ctgatggaag ataatcagaa ctgagcgttc
     2881 ccgtgtactc tgtagtgttt gcgctctctg attcgttgct tacgagaagg acattccccg
     2941 tataaaccgg cccgttcatg tctatgccgt cttcgggtgg tatttcggcg gcaactgttc
     3001 tgaacttcca gacatctccc tgggtagttg ctcccttagc gtctatagcc actatcttcc
     3061 agtagtaatc ctttccattc tgaagattgc cggggttgta agatttcgtc gttattcccg
     3121 aggccacctt ggatggattt gaactctctc cgaagtagac atcgtatttc agagagtctc
     3181 cgtctgggtc gctgcaattc catgaaagag ttatcgaaac agcgatatca ctgctgttat
     3241 ttgcgggatt ggggcttgaa ggcttcgcgg gtggctgatt gctctttgtg gtgaagttcc
     3301 agagatctcc ggtcgtctca agccccttcg aatccctggc aacgactttc cagtaatact
     3361 tcgtgccttc agcgagattc gaaggcgtgt agcttcttgc tgtctgcccg gccgaaactc
     3421 tggcagggtt tgtcgtggta ccgaagtaga tgtcatatac aagtgcgtcg ccgtcgggat
     3481 cagaacagtt ccatgaaagt gttatagatg ttccgacacc cgtagcaccg ctcgatgggg
     3541 ctggatttga aggcttcaga ggagcgttat tggaagactc aacagttatg ctgaaagtat
     3601 cggtcgcttc tccacccctg ccgtcgctcg ctctaacggt caccgaatag gttcctgcag
     3661 tggtcgcatt gtagctgaac tggtttccgc ttatcgttcc aactccggat actttggaga
     3721 acacaatagg atcaccatct gagtctgatg cgaagtttct caggtccaga accagattcg
     3781 ttccagtgct caccgtctga tccgggatct ccagagtggg gatcgcgttg tatctggcga
     3841 taactttctt cgggctgtta atgtttatct gaagagggtt atcatttagt gtctctccgt
     3901 tcaccgtcca gttgaggaag gagaagccct cgacctgcgg ggcggt
//
LOCUS       DAOQYI010000098         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_24363_length_3849_cov_77.128888, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000098 DAOQYI010000000
VERSION     DAOQYI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24363_length_3849_cov_77.128888"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PKL93_10940"
     CDS             complement(<1..1020)
                     /locus_tag="PKL93_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNQ71815.1"
                     /translation="MEPIRVYIVDNGTAEIKTLSERISKLRWNMHVETVSEDPLVGET
                     SWSNKFYTLNKYLKRKYEGFASLIDDMIFLVSLDDPMRPGRIVEANNSAAEKLGYSYD
                     ELLEMSFYSVAGDLDSQIKKIRADLQIKGMCTTDSFLTMKSGEKLQVEICLKVLDLQN
                     SIVTMVVARDTSAYRKVAAELQWRQNFERVISEVSGALKEYENIDDAIEKALGQLGEL
                     TSADRSNVFLFDYRTNSTRNTHEWCKEGVKSAKPHLQNMPFEEFSWWIKEMKKGGVHS
                     YVVSELPEEASREAEILAQQGVVSLVVIPIFSRQMPVGFVGLERLSGQFDWSRADIEL
                     LEVFAG"
     gene            complement(1055..2164)
                     /gene="nagA"
                     /locus_tag="PKL93_10945"
     CDS             complement(1055..2164)
                     /gene="nagA"
                     /locus_tag="PKL93_10945"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNQ71816.1"
                     /translation="MRFENVLVVDPIDGEFVGSVETEENVISEIKRVKGTQEFERILM
                     PGFVDPHTHGSVGVDTLSMSQKDLEKWENFLYSQGVTYFLPTTMSSTPSAILSAARVV
                     RDYIKNNSLTSVGGIHYEGPYLSLKRKGAQNPELIRSIDLKEIEETLIESVKLITMAP
                     ELEGFSHAQELIQKRGITLSLGHTDATYEDMEKAYNQGCDRITHFPNGMNTLHHRKLG
                     CVGAVLSLPFSVEMIMDGIHSLPGFVKLIFGIKGAEKIMIVTDTIDATAMPDGEYELG
                     GQKVILKNGRPTLEDGTIAAAVLVFSGAVRNFREFTDCTLKELAMVSSLNALNSMGIK
                     DRGRISKGYRADMVLLDENLGVRETILNGKTVFKS"
     gene            complement(2195..3250)
                     /locus_tag="PKL93_10950"
     CDS             complement(2195..3250)
                     /locus_tag="PKL93_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ71817.1"
                     /translation="MTIEDFLNNAKQSVLFTAPSLEKDLSAFLEEHTERIKNLAKTAL
                     DKGYKQIYWVGSGNSWCNLYSGDYILKKMTDLPSDYYKSYDFIWTNPSRLDKDALVIL
                     ASFSGNTEDTAAALRFANGKGATTISFTSKPDSILAREADESIVYDSNGLFILPLAGA
                     FIFSLEIARLKGRDVSKLYEQIEIMPELLGRIYKEEESRAYRLARKYQESDLFYVLAS
                     GAAYAIGYKFGLTVFMENMRVNASFIETSEFRHGPAEILDGHKPLMVFLVGSDESRDM
                     SERVIKIAESNGAEIIRFDVKDYGDFDPLFAPFLLMIPLQWFAVYSAYYRGIFDLDER
                     VLMGRGKMSTGNQITWP"
     gene            complement(3286..>3849)
                     /locus_tag="PKL93_10955"
     CDS             complement(3286..>3849)
                     /locus_tag="PKL93_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ71818.1"
                     /translation="IEGLDVDMIVSDDLGGGFEAAQYLLELGHRRIGFLSDTLDISTN
                     YDRIAGYREALSKYGIEEKQSWVKLGGFTIEGAYKSGVSLLGQNKDITAVIATNNYMA
                     AGLLLAAKDMGIEIPQDLSVISFDDIVWFDLCNPPITSVAQDTREIGSIAAKRILMNM
                     RGQRYEKGLTRLPTRLIIRESCTSPRD"
BASE COUNT          965 a          989 c          766 g         1129 t
ORIGIN      
        1 tccggcgaag acctcaagaa gctctatgtc tgctctgctc caatcgaatt ggccactaag
       61 cctttccagt ccgacgaaac cgacgggcat ctgccgagag aaaatcggaa tgacgacaag
      121 tgaaacgact ccctgctgcg caagaatttc ggcttctctg cttgcctcct ctgggagttc
      181 actgactaca taagagtgta ccccgccttt tttcatttcc tttatccacc aggagaactc
      241 ctcgaacggc atgttttgaa gatggggttt agcgctcttg acgccttctt tacaccattc
      301 gtgggtgttc ctggtcgaat tcgtcctgta gtcaaacaag aagacattgg atctgtcagc
      361 ggaagtcagc tcgcccagtt gtccaagcgc cttttctatc gcatcgtcta tgttctcata
      421 ctcttttagc gctccggaaa cctctgaaat cactcgttca aaattctgtc tccattggag
      481 ttctgcggcc accttcctgt atgcactcgt gtctcgggcc acgaccatag tgactatgga
      541 gttctgaaga tcaagcacct tcaagcaaat ctctacttga agtttctccc cgctcttcat
      601 agtgagaaac gaatcagttg tacacattcc cttaatctga agatcggccc ttattttctt
      661 tatctgagaa tcaagatcac cggccaccga atagaaactc atctcaagaa gctcgtcgta
      721 ggagtatcca agtttttcgg ctgcactgtt gttagcttcg actatcctgc cgggcctcat
      781 aggatcgtcg aggctcacaa ggaagatcat atcgtcaatc aggctggcaa acccttcata
      841 ctttctcttg agatacttgt tcagcgtata aaacttattg gaccatgagg tttctccgac
      901 caatggatcc tctgaaaccg tctctacgtg catattccat ctcaacttgg aaatacgttc
      961 agacaatgtc ttgatctccg ccgtaccgtt gtcaacgatg tacactctta ttggttccat
     1021 aagaccctcc gcagctgaat agataagaat ctattcagga tttaaataca gtcttcccgt
     1081 tcagaatggt ttctctaaca ccaagatttt catccagtaa taccatatct gcgcgataac
     1141 ccttggagat tcttccgcga tcctttatac ccattgaatt cagagcgttg agagatgata
     1201 ccattgcaag ttccttcaat gtacagtcag tgaattctct gaaatttctt acagctccgc
     1261 tgaagacgag tacggccgcg gcaattgttc cgtcctcaag agtaggtctt ccatttttca
     1321 gaatgacctt ctgtccgccc agttcatact ctccatcggg catggctgtc gcatctatag
     1381 tatcggtgac tatcataatc ttttctgcgc cttttattcc aaagataagc ttcacaaaac
     1441 ccggcagaga gtgaatacca tccattatca tctctaccga aaaaggcagc gaaagaacag
     1501 ctccaacgca gcccagtttt ctgtgatgaa gagtgttcat cccgtttgga aagtgggtta
     1561 ttctatcgca gccttggtta tatgctttct ccatgtcttc gtaagttgca tcggtatgtc
     1621 ccaacgaaag agttataccg cgcttctgta tcagttcttg cgcgtgtgag aatccctcga
     1681 gctccggagc catagtaatg agcttcaccg attcgattag cgtctcctca atttccttca
     1741 ggtctatgct tcgaatgagt tctggattct gagctccctt tcttttcaga ctcaggtagg
     1801 ggccttcata gtgaatgccc ccaactgaag tcagactgtt attctttatg taatctctca
     1861 ctactctagc agcagaaagg atggccgaag gagtcgatga catagtagtg ggcaggaaat
     1921 aagtcactcc ctgagaataa aggaagttct cccacttttc gagatctttt tgactcatgg
     1981 aaagagtgtc cacacccaca gaaccatgag tatggggatc gacaaatccc ggcatcagta
     2041 ttctctcgaa ttcttgagtc cctttaactc gcttaatttc cgagattacg ttttcttcag
     2101 tttctacact tcccacaaac tctccatcga tagggtcaac aaccaatacg ttctcgaatc
     2161 tcatcttcct tccatcctcc ctgtcataga tgaatcatgg ccaggttatt tgattacccg
     2221 tcgacatctt tcctcttccc ataagaactc tctcatcgag atcgaagatg cctctgtagt
     2281 acgccgaata tactgcgaac cactggagag ggatcatcag aagaaagggg gcgaagagtg
     2341 ggtcgaagtc tccataatcc ttcacatcaa agcgtattat ctccgcgccg ttagattcag
     2401 cgatttttat cacccgctcg ctcatatctc tcgactcgtc gctccccaca aggaagacca
     2461 tcagtggctt gtgaccatca agtatttccg cagggccatg tctgaactcc gacgtttcaa
     2521 tgaatgaagc gttgactctc atgttttcca tgaagacagt cagtccgaac ttatagccta
     2581 tggcgtaggc cgctccgctt gctaacacgt aaaagagatc actctcctga tacttcctgg
     2641 ccagtctgta agctcttgat tcctcttcct tgtatattct gcccagtagc tcaggcatta
     2701 tctctatctg ttcgtataac ttcgaaacgt ctctcccttt tagcctggct atttcaagac
     2761 tgaatatgaa ggctccggcg agtggaagta taaacagtcc attagaatcg tagactatcg
     2821 attcgtcggc ttcccttgcc agaatgctgt cgggtttgct tgtaaatgaa atcgttgtcg
     2881 ctccctttcc gttcgcaaat ctaagtgctg ccgcagtatc ttcggtattt ccagagaagg
     2941 aggcaagtat taccagcgca tccttgtcaa gtcttgaagg attagtccat atgaaatcat
     3001 agctcttgta gtaatcagag ggaagatcgg tcatcttctt gagtatataa tcacctgaat
     3061 agagattaca ccaggagttc ccgctaccca cccagtaaat ctgcttatag cctttatcta
     3121 aagcagtctt cgcaagattc ttgatccttt ctgtatgctc ttcaaggaag gcagaaagat
     3181 ccttctcaag agaaggggca gtaaagagta cgctttgttt tgcattattc aaaaaatctt
     3241 caattgtcaa ttcaaacacc tccgaatatc tatcaagaag tttagttagt ctcttggtga
     3301 ggtacatgat tcccttatga tgagtcgtgt aggcagtctt gttagcccct tttcatatcg
     3361 ctgacccctc atgttcatca atattctttt tgcggctata ctgccgattt ctctcgtatc
     3421 ctgagccact gatgttattg gaggattaca tagatcaaac caaacaatat catcgaagct
     3481 tatgaccgat agatcttgcg gaatctcaat tcccatgtcc ttcgcagcaa gaaggagtcc
     3541 cgccgccata taattgtttg ttgctattac cgctgtaata tctttgttct gacccaggag
     3601 agagacgccg cttttgtaag ctccctcaat cgtaaagccg ccaagcttga cccacgactg
     3661 tttttcttct atgccatact ttgaaagcgc ttccctgtat ccagcaatcc gatcgtagtt
     3721 tgtacttatg tcgagcgtat ccgaaaggaa accgattctc ctgtgaccaa gctccaaaag
     3781 atattgagct gcctcaaaac ctccaccgag gtcatcgctt acaatcatgt caacatcaag
     3841 gccttcaat
//
LOCUS       DAOQYI010000099         3847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_24390_length_3847_cov_55.969409, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000099 DAOQYI010000000
VERSION     DAOQYI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24390_length_3847_cov_55.969409"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..1897)
                     /locus_tag="PKL93_10960"
     CDS             complement(353..1897)
                     /locus_tag="PKL93_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNQ71819.1"
                     /translation="MKHLLLVLIVLSLFLFSGCGLLQYFTVAEAVEQETVEESVVEKL
                     KNSNGIEVPVFRGYFLNVDYSNPELFLQCGPQTRITDEIAAIADQFNDKKDLSTIKEI
                     YGWMMRNLRGVEGEKFGRTSHEIITSGGVTGCTDYGLAFASLARAKGIPSVFIQTARI
                     DWIEARKSSNSGMIVGHILVEVFIEGKWYLVDSTSGRLYLNYDRNNFSLPDGYYVFAK
                     SIEVWDSGAKNERENWQMMMDLFWIFDPSVYNNPGYDYIDLTTGETKSSGVFTGHSTV
                     IGSSALIFGKRSPVGLFDDKFTAHLSNKRLLDLYFADKDEVMSTQRKVVLHSLDQPAT
                     MPDYMLGIIPEFSSSETPVILNEIRDGRRIVLIMTDTTDELMKIIKELPEDFLDGPLN
                     DENVPDVEIMKDSVLVYGTSNSVGVFEERFIKGFSRKGFLSLGNSQYERFLETELVVV
                     LYSLDYPDKMPFYIEEYLPEFSSSELPVIVNEWREGKQIILIKTSTEAELINLIGQLP
                     DDFLLR"
     gene            complement(2060..>3847)
                     /locus_tag="PKL93_10965"
     CDS             complement(2060..>3847)
                     /locus_tag="PKL93_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71820.1"
                     /translation="VFLSLFTLIALGGCSLFRAIKIEIPDQQVTEGQELVVDLNQFVL
                     SSVSDGISFSVSSGPGRIDGKNYVFTSLPGDSENSPFNVRVSASDEGKSGVAEFLINV
                     NPIPEGEKAEIKLSGETAEESEEIIDDFVKRNPENPEGHAIRGAMKAIPELLKLLEIY
                     NSQIGSLDIAGTFDRSEILSGFEELVSNPDQVVQSILADIRARLLNMKLRSALILRNP
                     ELLVNFLASSSSVAENSGYSTCGEIGDFQDEMEEYADSLQEVWDDISYLSGEMKIVVK
                     VFLNEFDWDKDGVVESDKELMLEVVSEDNSITHTESFRLYTDILFLEKEPKGDHIVSI
                     EVDPDGGDAVFDLDFWFMISQGLEIEYAPGFDENDYLVIDEGEIAILKMLVGSALSSA
                     RAFTVYDLEPSEAFMEYLEDADDEIDPPEIDTNNDGNIDASEVKAFIGTSFLTFRNPS
                     KSPLWLSSIRDILLEAPETTMLLADDLLSDTQTEDNKHNLTSQWYIPFSQNAYDAMEE
                     NADEQRELLDYIRPNGEDLDTEIPVDEDEKTIILRLYNIFDHPERFSDLLSFFPTLIN
                     YEMLEGIEFPDPTFGGVVVNLPKTISPDN"
BASE COUNT          978 a          950 c          819 g         1100 t
ORIGIN      
        1 tgcacgggat ctcggttttt gaaggcgcag ccgggcggag cgggtaggtg gtaggaagag
       61 caaaagaaac tggtttgccg ccggttctcc gttcgccggg aaatgaagtg aaaaaccgca
      121 gcgataacag gtgaagattt catgcgtcga gtcacccatt tctgttcgta ccgagactct
      181 tttacctaat caagtacatg aaatcggcag tcctgaacca cttgtatctc gctttatatt
      241 agcgttttcg agtgtttctt caaattagta tactggtcag taacttactg tgcagtatac
      301 agatcgtttt ttcgctgtat cgggactttt attagtgaag cttaatcctt ctctacctca
      361 aaagaaaatc atccggaagt tgtccgatca aatttatcag ttccgcctcg gtagatgtct
      421 tgattaagat tatctgctta ccttctctcc attcgttcac tattacaggc agttccgaag
      481 acgagaactc cggcagatat tcttcgatat aaaatggcat tttgtcggga taatcgagtg
      541 agtagaggac tacgacgagt tccgtctcca gaaatctttc gtattgtgaa ttgccaagac
      601 tcagaaagcc ctttcttgaa aatcctttta tgaatctctc ttcgaatact cccacagagt
      661 tgctcgttcc ataaactaga actgagtcct tcattatttc gacatcaggg acattttcat
      721 cgtttagcgg gccgtccaaa aagtcctcgg gcagttcttt gattattttc atcaattcgt
      781 ctgttgtatc tgtcattata aggacaattc tcctgccgtc cctgatttcg ttcaggatta
      841 cgggcgtttc cgaagacgag aactccggta taatgcccaa catgtagtcg ggcatcgtgg
      901 cgggctgatc aagagagtga aggactactt tcctctgagt actcataact tcgtctttat
      961 cggcgaagta tagatcgaga agcctcttgt tagaaagatg cgccgtgaat ttatcgtcga
     1021 acagtccaac cggactccgc ttcccaaaaa tcagagccga gcttcctatg acggttgagt
     1081 ggccggtgaa gactccggat gacttcgtct cccctgtcgt gaggtcaata tagtcgtatc
     1141 ccggattgtt atagactgaa ggatcaaaga tccagaagag atccatcatc atctgccagt
     1201 tctctctctc gttttttgcc ccggaatccc atacctcaat ggatttcgca aacacatagt
     1261 acccgtcggg gagtgagaag ttgtttctgt cgtagttaag gtaaagcctg ccagaagtcg
     1321 agtctacaag ataccatttt ccttctataa agacttctac aagaatgtgc cctactatca
     1381 tgccgctgtt tgagctcttt cttgcctcga tccagtcgat ccgtgcggtc tggatgaaga
     1441 cggaaggaat cccctttgct cttgcaagcg aggcgaaagc tagtccatag tctgtgcagc
     1501 ccgtcacccc tcctgacgtt atgatctcgt gtgatgttct accaaacttc tctccctcaa
     1561 cccccctcag gtttcgcatc atccagccgt atatctcttt aattgttgaa agatccttct
     1621 tgtcattaaa ctgatcggcg atcgcggcaa tctcatctgt gattcgggtc tggggaccac
     1681 actgcaaaaa caactccgga ttagaataat cgacattgag aaaataccct ctgaaaacgg
     1741 gaacttcgat tccgttgctg ttctttagtt tctcaacaac tgactcctct acagtctctt
     1801 gctcgacagc ctcggctacg gtgaagtact gtaataggcc gcacccagaa aagagaaaga
     1861 gactcaatac aatcaaaaca agtagcaggt gcttcattgt aacacctccc gatgatcttc
     1921 tttcagaagc cttaagaaaa cccattactc ctttggaaac tcaacaagca gtatcatgtt
     1981 ttgattactt ctaacaaatg gggcaacgaa ccgttttcta caactcgttg ccccacaatt
     2041 atctcgattg agtccgacct cagttatccg gcgagatcgt cttcggaagg ttgactacga
     2101 cgccaccaaa cgtcggatca gggaattcaa ttccctcgag catctcatag tttatgaggg
     2161 tgggaaagaa gcttaagaga tctgaaaaac gttcggggtg atcgaagatg ttgtagagtc
     2221 tcaggataat agtcttttca tcttcatcga ccgggatctc agtatcgaga tcttctccgt
     2281 tcggccttat gtagtccaga agttcccgct gttcgtccgc attttcttcc atggcatcgt
     2341 aagcattctg actgaacggg atgtaccact gtgaagtcag gttgtgctta ttgtcttcgg
     2401 tctgagtgtc gctcaagaga tcgtctgcca gaagcatcgt agtttcaggt gcttccaaaa
     2461 ggatatccct tattgaactc aaccatagag gacttttgga ggggttcctg aatgtcaaga
     2521 aggatgtgcc gataaatgcc ttgacttctg acgcgtcgat atttccgtca ttatttgtat
     2581 cgatctccgg aggatctatc tcgtcatcag cgtcttctaa atactccatg aaagcctccg
     2641 atggctcgag atcataaacg gtgaatgctc tagcgcttga aagggcactg ccgaccagca
     2701 ttttcagaat tgctatttct ccttcatcga ttacaagata atcgttttca tcgaatccgg
     2761 gagcgtactc tatttcaagt ccctgtgaga tcatgaacca gaaatcgaga tcgaacaccg
     2821 catctccgcc atcgggatcg acttcgatcg agactatgtg atctcccttc ggctctttct
     2881 ccaggaaaag gatatccgta tacagacgga agctctctgt gtgggttata gaattgtctt
     2941 cgctcactac ttcgagcatc agctctttat cgctttcgac aactccatct ttgtcccagt
     3001 cgaattcgtt gagaaatact ttcacaacaa tcttcatttc tccactcaaa taggagatat
     3061 cgtcccagac ttcctgaagg ctgtcagcgt actcctccat ctcatcctga aaatcgccga
     3121 tctccccgca agttgaataa ccggaattct ctgctacgga gctgcttgaa gcaaggaaat
     3181 ttaccagaag ctccggattt ctgagaatca acgcagatct cagcttcata ttcagcagtc
     3241 ttgcacgaat atctgctaaa atgctttgaa caacttgatc tggattcgaa acaagttctt
     3301 cgaaaccaga cagtatttca cttcgatcaa atgttcctgc aatgtcaaga gaaccaatct
     3361 gtgaattgta tatctccaga agtttcagta attccggaat cgccttcata gctccacgaa
     3421 tggcgtggcc ttcgggattc tcaggattcc tcttcacgaa gtcgtcaata atctcttcag
     3481 actcttcggc agtctcccca gataacttga tctcggcctt ttcaccttcc ggaatgggat
     3541 ttacgttgat caggaactct gctactccgc tcttgccttc atcacttgcg ctcactctca
     3601 cattgaaagg gctgttttcg ctgtcaccgg gcaaagacgt aaatacatag ttctttccgt
     3661 cgattctccc gggtccgctg cttaccgaga atgatatccc gtcggagaca gatgataata
     3721 cgaactgatt gaggtcaaca accagttctt ggccctctgt cacctgctga tcgggaatct
     3781 caatcttgat agctctgaat agagagcagc cgccgagcgc tatcaaggtg aatagagaaa
     3841 gaaacac
//
LOCUS       DAOQYI010000100         3802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_24814_length_3802_cov_80.894049, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000100 DAOQYI010000000
VERSION     DAOQYI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24814_length_3802_cov_80.894049"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1723
                     /locus_tag="PKL93_10970"
     CDS             320..1723
                     /locus_tag="PKL93_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNQ71821.1"
                     /translation="MKKALLLFSLVVLFALTALAGPKYVFLFIGDGMALPQINAAQLF
                     LSSSFEDAVTPKLNMLRFPVQGMTTTYSSNSFITDSASAGTAIATGRKTNDGVISMDP
                     TLTESYQTVAEFAKSRDMKVGIISSVSIDHATPAVFYAHEKSRNNYYSIGLQAVTSNY
                     DLFGGGTFNRPDNKGKDRNLFEIAVENGFTVVRTQEEFESLESVEGKVIITNEVIPGS
                     GAMAYEIDRENEFSLADITAKAIQLLSNDNGFFIMVESGKIDWACHANDAAASVGDTV
                     AFDKAIAEAIKFYNEHPEDTLIVVTGDHETGGLTLGFAGTKYVANVPTIGNQKMSYEA
                     FDALLSSEKALGKLNTFEDLLVLVEDNFGLVTTGKPQGIPLNDTEIERLREAFGYYVN
                     GSVPKDSRTYILYGGYNPVSVTLTHILNEKAGLGWTSYSHTGVPVATFAMGVGQEMFA
                     GFYDNTDIGKNLFKLLD"
     gene            1788..3011
                     /locus_tag="PKL93_10975"
     CDS             1788..3011
                     /locus_tag="PKL93_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNQ71822.1"
                     /translation="MRKVSISGSKKRYELLFVLILVAVNIVLIIIPNRYDKSSNYSYT
                     VGKIVEVDNTELKQYGLIKQGSQYLTVEIKRGELKGQVFETDNNLIGKMELDKVLSEG
                     DTVYVEYSTIGDGSIYVKVVDFYRLGTEMLLFTVFGVMLILFAGFTGLKTLLSFTTTL
                     LVLWKVMIPLYLQGFNPVLIAFAIVSALTLAIIFLVGGFTRKGFVAFLGSISGVLVTL
                     ILSLSFSDPFYINGSVMPFAETLLYSGFAHLDLNQLFLASIFIGSSGAVMDIGMDLSA
                     SMQEVVFKNPDITTRELMFSGFRVGRAVVGTMTTTLLFAYSGGYLTLLMVFMAQGVNT
                     SAMLNLNYFSSEVLITVIGSFGLVLTAPLTAIIGAFVFTRKGSELLIPANQEAGQSVD
                     KKARELGPAINDSVD"
     gene            3041..3634
                     /locus_tag="PKL93_10980"
     CDS             3041..3634
                     /locus_tag="PKL93_10980"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71823.1"
                     /translation="MSEDLPYTFNTSDWLPTQGVTDTLPIVAWFPPVFYHFNVVANPP
                     EGGEVMESGVFIYGAPMTLGAIPNDNYVFRNWTFEGAEFSDNPLWEGNSTFFLARSTC
                     IGCTEFTIVGNFDLDVPDTITVNLRAEPPEGGEVSGGGKFSKGSSTTISAVPNSGYEF
                     SGWYFDPCGSPFSDSQTLTLSNLQVDWALFASFHKPF"
BASE COUNT         1036 a          834 c          878 g         1054 t
ORIGIN      
        1 agatttggtt tgcctgatga ctgtgagaca agctccaggt gacatcttga acatcagtta
       61 ttgatttacc cagtgatttg tcgagaagac tatcaagcgg agattacctg aaagcctttc
      121 ttcggtgcct tgtcaagacg ctcttttcaa atgtctcgca gtagttctag aagccacttg
      181 tttcgcatac atgacttact tttgctttcc attaagtgat taggcatctc gaatattcag
      241 aatctactgt ggaaaaaact gcactctaga attctctatg gatagtaatc gggtcaaatt
      301 ctttcaggag gtaagtatta tgaagaaagc actgctactt ttctcgcttg tggttctatt
      361 tgcgttaacg gctcttgcgg gtccaaagta tgttttcctg tttattggcg atggcatggc
      421 tcttccacag atcaacgcag cacaactatt tctaagttca tcatttgagg atgcggtaac
      481 tcctaagcta aacatgctta ggtttccagt ccagggtatg acgacaacct actcctcaaa
      541 ctccttcata accgactcag cctcggccgg tactgcgata gcaacaggaa ggaagacgaa
      601 tgatggcgtt attagcatgg atccaacact tactgaatcc taccaaacgg ttgcagaatt
      661 tgccaaaagc agggacatga aagtcgggat catttcaagc gtctccatcg accatgccac
      721 gccggcagtt ttttacgctc atgagaaatc aagaaacaat tactactcaa ttgggcttca
      781 ggcagtaaca agtaattacg acctcttcgg tggcggaact ttcaacaggc ctgacaacaa
      841 aggtaaagac agaaatctct ttgagatagc tgtagaaaat ggattcacgg ttgtaagaac
      901 tcaggaagag ttcgaatcac ttgaatcagt tgaaggaaag gtcattatca caaacgaagt
      961 aattcctggc agcggagcga tggcttacga aatcgataga gagaacgaat tctctctggc
     1021 agatattact gcaaaagcaa tacaacttct ctcaaatgac aatggcttct tcataatggt
     1081 cgagtcaggc aagatagact gggcgtgtca cgcaaacgac gcagcagcat cagttggaga
     1141 tacggtagct ttcgataagg caatagcgga agcaataaag ttctacaatg agcatccgga
     1201 agataccctt attgttgtta ccggtgacca cgaaacgggt ggactcacac ttggtttcgc
     1261 tggaacaaag tacgttgcca acgttccaac aatcggaaat cagaagatgt cctacgaggc
     1321 cttcgatgct ctcctctctt ctgaaaaggc tctgggcaaa ctcaacacgt tcgaagatct
     1381 tctcgttctt gttgaggaca actttggtct tgtgaccact ggcaaacctc agggcatccc
     1441 tctaaacgat acagagattg aaagacttcg ggaagccttc ggttactacg tgaatggtag
     1501 cgtaccaaag gatagcagaa catacatcct ctacggtggt tacaatccag ttagcgtaac
     1561 actcactcat attctgaatg aaaaagccgg actcggttgg acatcttact cgcataccgg
     1621 tgttccagtt gcaacattcg ccatgggtgt tggccaagaa atgttcgcag gtttctacga
     1681 caataccgat atcggcaaga atctcttcaa actccttgac taatagtatc gataaatgta
     1741 gtggccagtg cgcagaaaaa cgcactggtc tcttgttctg attggagatg agaaaagtga
     1801 gcattagtgg atccaaaaaa cggtatgagc tcctctttgt attgatcctg gtggctgtga
     1861 acattgtgct catcatcata ccaaacaggt atgataagag ttcaaattac agctacacag
     1921 ttggaaagat tgttgaagta gacaataccg aactcaagca gtatggactg ataaaacagg
     1981 ggagtcagta tttgaccgtt gagatcaaac gcggcgaact gaaagggcag gtttttgaaa
     2041 cagacaacaa ccttataggt aaaatggaac ttgataaagt actcagtgaa ggcgataccg
     2101 tctatgtcga atactcaacc atcggtgacg gatcaatcta cgttaaggtc gtcgatttct
     2161 accgtcttgg tactgaaatg cttcttttca cagtattcgg agtaatgctt atactcttcg
     2221 cggggttcac cggactaaaa acactactct cgttcactac cacgcttctg gttctgtgga
     2281 aagtaatgat tccgctctat ctacaaggct tcaatcctgt attgatagct ttcgcaattg
     2341 tttcagcact cactttggca atcatctttc tggttggagg attcacccgt aagggatttg
     2401 ttgccttcct tggatcgata tcgggagtct tggtaacact gattctttct ttaagttttt
     2461 cagacccatt ttacataaac ggttcggtga tgccgtttgc ggaaacactg ctttattcag
     2521 gttttgcaca ccttgatctg aaccagctct ttctggccag catttttatc gggagttcag
     2581 gggcagtaat ggatatcggt atggatcttt ctgcatctat gcaagaagtg gtattcaaaa
     2641 accccgacat taccacaaga gaactgatgt tttccggatt tcgtgtaggt agggcggtgg
     2701 taggcacaat gacgaccact cttctttttg cctactcagg tggctatctg actcttctca
     2761 tggtcttcat ggctcagggc gtgaatacat cagcgatgct gaatctcaac tatttttcgt
     2821 cagaagtact cataactgta atcgggagtt ttgggctggt tcttaccgcg cctctcacag
     2881 ccataatcgg agccttcgta ttcaccagaa aagggagcga gcttcttatc ccagcgaacc
     2941 aagaagccgg acaaagtgtg gacaagaagg ctcgagaact tgggcccgcc attaacgatt
     3001 ccgtcgattg aggaatggct ggtacaccga cggggaaaag ttgagtgagg atcttccata
     3061 cacatttaac acatctgatt ggctgccgac acagggtgtg accgataccc ttccgatagt
     3121 agcctggttc ccgccagtct tctatcattt caacgtagtt gcaaatcctc ccgaaggcgg
     3181 ggaagtcatg gaaagtggtg tattcatata cggcgctcca atgacgctgg gtgcaattcc
     3241 taatgacaat tacgtattca ggaactggac tttcgaggga gctgagttca gcgataatcc
     3301 attgtgggaa gggaattcca ccttcttcct tgctagatcg acttgtatcg gctgcactga
     3361 attcaccata gtcggcaatt tcgacctgga tgtgcccgac acaattactg tgaacttgag
     3421 agccgagcca cctgagggtg gtgaagtaag cggcggaggc aaattttcaa aaggatcgag
     3481 cactactatc agcgccgtgc caaattccgg atatgagttt agtggctggt actttgatcc
     3541 atgtgggagc cctttcagtg attctcagac tttgactcta agcaatttgc aggttgattg
     3601 ggctcttttt gcgtctttcc acaagccttt ttagatcgaa gctctctttt tgagacatag
     3661 ctttttagtt tatttgattt tgtgtacaaa gaaggagcct gaggagagaa tcctcaggct
     3721 tctatctttt caacaaaacc gattctgaaa ccgtccggat ctctgatgac gaatttaagg
     3781 attccatagg gagtcgtttc ga
//
LOCUS       DAOQYI010000101         3760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_25224_length_3760_cov_79.550067, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000101 DAOQYI010000000
VERSION     DAOQYI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25224_length_3760_cov_79.550067"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKL93_10985"
     CDS             <1..810
                     /locus_tag="PKL93_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNQ71824.1"
                     /translation="MLYVVFIGVLMGLANLIPGVSGGTIALLGGLYERFVGSISSVSA
                     FKIRKEDVVFLIEIVAGIVIGILGFSTLIDVSLSRVPSLMYGIFSGLVIGGTPVVFKR
                     IEKFDSSSFSFLVIGGLLVYLVSILASGPGKEAVLNHNTASLLYDVFAGFFGAAAMVL
                     PGLSGAFILLIMGEYTRAIAALRHFDFIIIVFIGVGVILGIVFVSRLMKFLMKRFRSQ
                     TFAFLLGLMIGSLPDLFTRPGSNSNVLQIIIGLVSGLAISYLLSMFEKKTE"
     gene            complement(819..1829)
                     /locus_tag="PKL93_10990"
     CDS             complement(819..1829)
                     /locus_tag="PKL93_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNQ71825.1"
                     /translation="MKKGDLGIDLGTASLLVFQKGKGIVIDEPSVIAVEAKTGKIIAI
                     GSDAKEMIGKTPKDIKAVRPIRDGVIADYQIIEQALKDLVKRTRTKFSFSRPAVVVGV
                     PAKVTSVERRAVIEATTAAGASKVYLVLEPIAAAIGAGLHIFDSVGNMVVDIGGGTSD
                     IAVISLGGIVVSRSLRTAGDAMDDAIIKFVKRKYKFLIGSSTAEDVKTKIGKAFPTLE
                     SFELEVRGRDALNGLPGNIRITSDDVHEAISQILQEIVLNLRQILEDTPPEIAADIMD
                     TGVVLTGGGSLIRGLPDLIIQETGIKTVVAEDPRTCVVKGIGELLDCDKRLHRVAINH
                     SK"
     gene            complement(1854..1929)
                     /locus_tag="PKL93_10995"
     tRNA            complement(1854..1929)
                     /locus_tag="PKL93_10995"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1894..1896),aa:Arg,seq:ccg)
     gene            complement(1908..2618)
                     /gene="ruvX"
                     /locus_tag="PKL93_11000"
     CDS             complement(1908..2618)
                     /gene="ruvX"
                     /locus_tag="PKL93_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNQ71826.1"
                     /translation="MIRLGIDFGTSRIGLALQVENIEIPLFAIDHTGYKKNLLRIIEE
                     KGIEEIVIGLPISMSGRFSESTLKAVSFAEKVKSIFPGRVFLVDETLTTETARRLSSE
                     AGQDFSKARDVFSAIQILRNYSSGMSKKWEVKEERGVCRDLPRLASESRVLFYRPRSA
                     MIEGLDCLETEPGVLVEDPQVFLSFVRKGMKPVNIVDDIDFSSYDIIVIACGEELDGM
                     VDLNSEGPQVIECSWLNG"
     gene            complement(2611..2871)
                     /locus_tag="PKL93_11005"
     CDS             complement(2611..2871)
                     /locus_tag="PKL93_11005"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNQ71827.1"
                     /translation="MVDTRKLDALIVVSQKDIIKVLSLLRNHGFQTKVFPTPPGLFPG
                     CSLSIAVSSNVVDSVLVLLKQHNVEVMYSALCDENPVKSFYD"
     gene            complement(2864..>3760)
                     /locus_tag="PKL93_11010"
     CDS             complement(2864..>3760)
                     /locus_tag="PKL93_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNQ71828.1"
                     /translation="VLGISGFSGSIFCDDLKEFFLDKRISHVYKPSPIKRVGIIDNRG
                     SRKKVEQVMSIVGHGENVAVIVDSPHKTVSLAKSMGSRLSHALQNGELIFYNYLLKDF
                     QRSVIYSLVERQKIRVLITTPSSDGLGVTLGNSNIVFYSAPRNFLEVIDAVATRPGED
                     SELFLNLSFNKADLVSNTSEMDRLFPTVEELEAVYQDLKEVLPASERDIKKALSFEDG
                     ISKVFLSMLEEMELLSADNGVWYSNSEAALDTTQVKKTLRYREGVAEKRMTRWFATKL
                     STMTTRGLLRSLTNAEEVLKVG"
BASE COUNT          947 a          920 c          800 g         1093 t
ORIGIN      
        1 atgctctatg tagtctttat aggtgtcctc atgggtcttg ccaatcttat tccaggagtg
       61 agcggaggca ctattgcact ccttggaggc ttatatgaac gtttcgtggg ctcgatttcc
      121 tcagtgtccg cttttaagat tcgcaaagaa gatgtcgttt tcctcatcga aattgttgcg
      181 gggattgtta taggaatcct tggcttttca accctaatag atgtttctct ttcgagagtc
      241 ccttctttga tgtacggtat cttttcggga cttgtcatag gaggaactcc cgttgtattc
      301 aagagaatcg agaaattcga ttccagctca ttttctttcc ttgtaatagg gggtttgctc
      361 gtctatcttg tttccattct tgcttcaggg ccgggaaaag aagctgttct aaatcacaat
      421 acagcgagcc ttttgtatga tgtttttgct ggattcttcg gtgcagctgc gatggttctg
      481 ccaggtctca gcggtgcctt cattttgttg attatgggtg aatacactag ggctatagct
      541 gcattaagac acttcgattt cataatcata gtgttcatag gagttggagt tatccttgga
      601 attgtattcg tgagcaggtt aatgaaattt ctgatgaaga gattccggag tcagacattc
      661 gcttttctct taggtctaat gataggttca cttcctgatt tatttacgag acccggaagc
      721 aattcaaatg ttcttcagat tattatcgga ttggtttctg gactggcgat ttcgtatctt
      781 ttaagcatgt tcgaaaaaaa gacagaatag aaccctgact acttactgtg attgatcgcg
      841 actctatgaa ggcgcttgtc acaatcgagc aactcaccta taccttttac gacacaggtt
      901 ctcggatcct ctgcaacaac tgtctttatt ccagtctctt gaataatcaa gtctggaagt
      961 cccctaatta gggaaccacc acctgtaaga accacaccgg tatccattat gtccgcagcg
     1021 atctcaggag gcgtatcttc caaaatctgg cgcagattca atacgatctc ctgaagaatc
     1081 tgagaaatgg cttcgtgaac atcatcggaa gtgattctta tgttcccggg aaggccattc
     1141 agtgcgtctc ttccacgtac ttctagttca aaactctcca gcgttggaaa ggcctttcct
     1201 attttggttt ttacgtcctc tgcggttgag ctgcctatga gaaacttgta cttgcgtttt
     1261 acgaatttga ttattgcatc gtccatagca tctccagcag ttcttaggga gcgcgaaaca
     1321 actatcccgc caaggctgat caccgcgata tcggaggtcc cccctccaat atcgacaacc
     1381 atgtttccta ccgaatcaaa aatgtgaaga ccggctccga tggcagcagc gatgggttca
     1441 agaacgaggt aaaccttact tgcacctgcg gcggtggtgg cttcaataac ggctcttctc
     1501 tcaacactag ttaccttcgc cggaaccccg acaacaacgg ccggcctcga gaaagagaac
     1561 ttcgtacggg tcctcttaac aagatctttc agggcctgtt ctattatctg ataatcggcg
     1621 atcactccgt ctcgaattgg ccttaccgcc ttgatatcct ttggcgtctt cccgatcatt
     1681 tccttagcat cggatcctat cgctataatt tttccggtct tggcttcaac agctataaca
     1741 gacggttcgt ctatgacgat gcccttgccc ttctggaaga ccaagagaga agcggtaccc
     1801 aaatcgattc ctaaatcgcc ttttttcata caagacctcc cgcgttgcct atatggcgtg
     1861 ctcggtggga tttgaaccca caatctctgg atccggagtc cagcgctcta tccgttgagc
     1921 cacgagcact caataacctg cggaccttcc gagttcagat cgaccatccc atcgagctct
     1981 tctccacaag cgattactat tatatcgtaa gatgaaaagt caatatcgtc aacaatattg
     2041 acgggtttca tcccttttct tacgaaagaa aggaagactt gaggatcctc gacaaggaca
     2101 cccggctcag tttcaagaca atcaagacct tcaatcattg cagatcgcgg cctataaaac
     2161 aacactctac tttctgaagc cagcctgggt aggtctctgc aaacgcctct ttcttctttt
     2221 acctcccatt ttttcgacat tccggacgaa tagttccgca ggatctggat agcactgaaa
     2281 acatccctcg ctttagagaa gtcttgtccg gcctcactcg aaagacgcct ggcagtttcg
     2341 gttgtaaggg tctcatcgac caggaatact cgtccaggga agatactctt gactttctct
     2401 gcaaatgaaa cggccttcag agtggactca ctgaatctac cggacattga tatggggagg
     2461 ccgattacaa tctcctcaat tcccttctcc tcaataatcc tcagaagatt ttttttgtag
     2521 ccggtgtgat ctattgcgaa tagaggaatt tcgatgttct caacctgtaa tgccagtcct
     2581 attctggatg ttccgaaatc aatccccagt ctaatcatag aatgacttca cgggattctc
     2641 atcgcacaaa gcggaataca taacttctac gttatgttgc ttgagaagca ccagaactga
     2701 atcgacaaca tttgaagaaa ctgcgatgga aagcgaacat ccggggaata gaccgggtgg
     2761 tgtggggaaa acctttgtct ggaaaccatg attcctcagt aatgatagaa ctttgattat
     2821 atctttttgg gagactacta tcagagcatc cagcttacgg gtatcaacca accttcagca
     2881 cctcttcggc attggttaga ctccttaata gcccccttgt agtcatagta gataactttg
     2941 tagcgaacca tctcgtcatt ctcttctctg ccacaccttc tctgtatctc agggtcttct
     3001 tcacctgtgt cgtgtcgagg gctgcttccg agttactgta ccagacaccg ttgtccgcac
     3061 tcaacaactc catttcctca agcattgaga ggaagacctt agagataccg tcttcaaagc
     3121 tcagagcctt ctttatgtct ctctccgacg cagggagtac ctctttcaag tcctgataaa
     3181 cggcttccaa ctcctcaaca gtgggaaaca aacggtccat ttcgcttgtg tttgaaacga
     3241 ggtccgcctt gttgaaggag agattcaaga aaagttcgga atcttcaccg ggccttgtag
     3301 caacggcatc gattacttcc aggaagtttc tcggagcact gtaaaagaca atatttgaat
     3361 tgcccagtgt aaccccgaga ccatcacttg aaggagtcgt tataaggacc cgaatcttct
     3421 gtctttcgac aagagagtag ataaccgatc tctggaaatc cttgagaaga tagttgtaga
     3481 aaatcagttc accgttttgc agagcgtgag acaaccttga acccatagat tttgcaagag
     3541 aaaccgtctt gtgaggagag tcaacaatta ctgccacatt ttccccgtgg cctactattg
     3601 acatcacctg ctccactttc tttctcgagc cgcggttgtc tataatgcca actctcttta
     3661 ttggacttgg tttatacacg tgcgagattc tcttgtccag gaagaactct ttgaggtcat
     3721 cacagaaaat gcttccagaa aaaccggaaa tgccgagaac
//
LOCUS       DAOQYI010000102         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_27213_length_3568_cov_67.717051, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000102 DAOQYI010000000
VERSION     DAOQYI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27213_length_3568_cov_67.717051"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PKL93_11015"
     CDS             complement(<1..824)
                     /locus_tag="PKL93_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNQ71829.1"
                     /translation="MNDNYKDEISQKVARAYEQIAKTHCSSKSYSLGHIKLKTVELIH
                     LIRKTFCPQLSHTRPVDGEELLLEGLNTLREMIMEIRPELGEEGASQVVKEFLAELPS
                     VALTLVTDVRAAYEGDPAAKSVEEIMIAYPAYEAISTYRLAHILYKLDVPLIPRIMTE
                     YAHQKTGIDIHPGAAIGNYFFIDHGTGVVIGETCTIGHHVKIYQGVTLGAKSFELDEN
                     GNPIKGIKRHPDIGNHVVIYAGATVLGGNTVVGDNCVVGGNVWLVHSLKPGEKIYN"
     gene            1076..1630
                     /locus_tag="PKL93_11020"
     CDS             1076..1630
                     /locus_tag="PKL93_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71830.1"
                     /translation="MRRLLTVCFLALSIGLFARFEIGLPVFSMPQSDSKNGIVEVLTD
                     LSEENMNLEITVVFTDEDYPCPVINFLYDIYRYFKYGRIEDIESFYIRFDSDGKIIAL
                     DFPGVFAADHAFHDTKNLHGSAVLMPDVITFVDGRPLIFINTWNHMFGVIPSFDRANE
                     TLLSDYPVTEGTRSDAEHKYSWHH"
     gene            2200..2781
                     /locus_tag="PKL93_11025"
     CDS             2200..2781
                     /locus_tag="PKL93_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71831.1"
                     /translation="MKNLQKATSAEKRNELDELRNLVGSYSQSADDNHVHLNLRGPDL
                     TGDDAERAIDVLEEKTKYLPLKGNLTVMVDCDCGDLHDFSIKIKDSIRKQLIPLLYYV
                     DLDLRRLSRVSVVLMIFGVLAMGTASLLSELSFNAYALHEILVIASWVFIWASVETFF
                     FDRRRLKNKKHRLQRIYFADYVITDCQRENDYH"
     gene            2822..3175
                     /locus_tag="PKL93_11030"
     CDS             2822..3175
                     /locus_tag="PKL93_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="HNQ71832.1"
                     /translation="MAKKMPVVYWEINAVDGESLSEFYEEVFEWETEVSDTGFHSFKS
                     GDPKGINGGIFTGKGVLPTHRALYIEVEDMNEIIKRVQDHGQEILQGPFKSGNTVLAF
                     FTDPEGHMIGLIQRE"
BASE COUNT          964 a          751 c          829 g         1024 t
ORIGIN      
        1 ttgttgtaga tcttttctcc cggcttgagc gaatggacga gccagacgtt tccgcctacc
       61 acgcagttat ctccgacaac ggtgttcccg ccaagcacag tcgctcccgc gtatatcacg
      121 acatggttac cgatgtctgg atgacgtttg attcccttta tcggatttcc attctcgtcg
      181 agttcgaagc ttttcgcgcc aagcgttacg ccctgatata tctttacatg gtgtcctatg
      241 gtgcaggttt ctcctattac gacaccggtg ccgtggtcaa taaagaagta attcccgatt
      301 gctgcacccg ggtgaatatc tattccggtc ttttgatgag catactccgt cattatcctg
      361 gggatcagcg gaacgtccag tttgtaaaga atgtgtgcga gcctgtaagt gcttatcgct
      421 tcgtaggcgg gataagcgat catgatttct tccaccgatt tggcggcggg gtctccttcg
      481 tatgcggccc gcacgtctgt gactagagtc aaggcaacgg agggtagttc agccaaaaac
      541 tccttcacaa cttgacttgc tccttcttct cccaattcag gtcgtatttc cataatcatt
      601 tcccttagtg tattcaaacc ctccaacagt agttcttccc cgtcaacggg tcgggtatgt
      661 gaaagctgcg ggcagaaggt ttttcgaata agatgaatga gctctaccgt ctttagcttt
      721 atatgtccaa gtgagtaaga ctttgatgaa caatgagtct tggcgatttg ttcgtatgct
      781 cttgctactt tttgtgaaat ctcgtctttg taattatcat tcataagcat tatctcctaa
      841 aaaactcagt tgatttcttt cagtatagac gagaaatcat gagattgctc aacgagtttt
      901 gatcaatcaa tgtcattctg aaacctcggt agtcgatcgc gccgaggagc gtcgacttca
      961 aaggatacca aagttggcct aacataaggt cacgatattt gtggttaccc gagcgcacat
     1021 ggggtataaa atatgagtcg agaagcgaag aattattcat ggaggatgag gttatatgag
     1081 aagattgctc acggtatgtt tcctggcact gtcaattgga ttgttcgcaa gatttgagat
     1141 tgggctaccg gttttttcca tgccccaaag cgattctaag aacggaattg ttgaagtctt
     1201 gacggattta tctgaagaga atatgaatct cgagataact gtagtcttta ccgatgagga
     1261 ttacccgtgt ccagttatca atttccttta tgatatctat cgctatttca aatatggccg
     1321 tattgaggat attgagagtt tctacatcag gttcgattcg gatgggaaga taattgctct
     1381 agatttcccg ggtgtttttg cagctgatca cgcctttcat gacacgaaga atctacatgg
     1441 gtctgcggtc ctgatgcctg atgtaataac atttgtggat ggaaggccct tgatcttcat
     1501 caatacttgg aatcacatgt tcggagtaat cccgtccttt gacagagcta acgagacgtt
     1561 gctgtcagac tatcccgtca ccgaagggac caggtcggac gcggaacata agtactcctg
     1621 gcaccattag tagtctcgtt caggttgaag ggttttgctc taagctgagt gaattaccgt
     1681 tgctttcatt cttcttgaaa tgtttcaaga acagctggac gatcgaagtg cattctagat
     1741 tttttcttgt gtcgacacag ctttgcgaac aaggatatgc aagcagcgtc caagggaccg
     1801 cacgattata atccgttaag tcagcttgaa tggcagtttc ttgatctgaa tttctgtttg
     1861 tagaatagta atcgctgaag tcaaactgtc agaagaattg caagctggat tttgcttccc
     1921 tagcgcttag agtaatcatt cagctgcttt taagtcacaa tacctctcta agtcgctgtc
     1981 attgccataa aggaaaggga ataagaaacc tgtgatgtcc gaacactgat tggtcgcaga
     2041 gagtcaaatg gtttggaaga ggttcttttc cggttcactg tacatttcat actccccact
     2101 taacttcgtt ttgatgccca actagattta taatcattca ttcggaagaa aaactaaagg
     2161 gttattgaat aattagaaag tttagttcac ccgatggaga tgaagaactt gcaaaaggct
     2221 acctctgcgg aaaaaagaaa cgaactcgat gaactacgca atctagtcgg aagttattcc
     2281 cagagcgcgg atgataatca tgtgcatctc aacctaaggg gaccggatct tacgggcgat
     2341 gatgcggaaa gagcaataga tgtgcttgaa gagaaaacca aatatctgcc tttgaagggt
     2401 aatttaactg tgatggtcga ctgtgattgc ggtgatctgc acgatttctc gataaagata
     2461 aaagactcca taagaaagca gctgattcct ttgctttact acgtcgatct tgatctgagg
     2521 aggctttcca gagttagcgt tgtcctgatg atattcggtg ttctagctat ggggacggct
     2581 tcactgctct ccgagctttc tttcaacgcc tatgcgcttc acgaaatact cgtcattgcg
     2641 agctgggtct tcatatgggc atcggtcgag actttcttct tcgacaggag aaggctgaaa
     2701 aacaaaaaac accgtcttca acgcatctac tttgcagact atgtgattac tgattgccaa
     2761 agagaaaatg actatcacta atacgctgta aagcatttct aaataaatgg ggggaattaa
     2821 gatggcaaag aaaatgccgg ttgtgtactg ggaaatcaat gcagtagatg gggagtcgct
     2881 ttccgagttc tatgaggaag tctttgaatg ggaaacagaa gttagcgaca cgggatttca
     2941 ttctttcaag agcggagatc ccaaaggcat aaacggcgga atattcaccg gaaaaggtgt
     3001 acttccgact catagggcac tgtatattga agttgaagac atgaacgaga ttataaagcg
     3061 ggttcaagac cacggccagg agatacttca aggccctttc aaatctggta acactgttct
     3121 tgcctttttc accgatcccg aaggacacat gatcggattg attcaaagag agtgatgctc
     3181 tctggagcga agctccctct tggttacgct caacgtcttg acaacatgag ataagagcca
     3241 gagagagaat aacggttctt ccgcgctacc atcgcaagtt cttcctaaag agcgaagata
     3301 atagttccgg tgcttcgcac ctaagattct cgttccgaca gttagtctcc aggtttttgg
     3361 taaaaaaagc tgtatgagat cagacgtaaa aactctgaag agcgtggaaa tcttctcttc
     3421 tatcctcctt cctggcacct ctgaccataa ttgtgcctct tcaaatctct aagcaatctc
     3481 taagcggctt ttaagcgggc tccaagaaac ggaccgtaga ctgagaatgt accaaaaatc
     3541 atatggaggt gtaagagatg aaaaagat
//
LOCUS       DAOQYI010000103         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_27741_length_3519_cov_71.974885, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000103 DAOQYI010000000
VERSION     DAOQYI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27741_length_3519_cov_71.974885"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..1573
                     /locus_tag="PKL93_11035"
     CDS             266..1573
                     /locus_tag="PKL93_11035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HNQ71833.1"
                     /translation="MDRNLHRFISQTQGYILSLCDGTNTLNDIINQVARLYDKSFDEC
                     LEKVSGFLQEEVLSGAVNYLDRKLSAKINPYEEMKFMFVPQMADDFIVSEELESIGII
                     LTERCDYNCIYCFGSYGRASTQAKELTLDKAISIIREARSLSAEHIILSGGDPFEYRG
                     ILDILNVVRLEGFRSCEISSKGIRLTKEFVKDLKKTGLKRIQISIDSLNADSNDYLSG
                     TARSLEYAFRAIFWSRWYGIDVSARSTVTRMNYKEIGAFIDSLCHMGIKQIRLVSVIP
                     VGKALAEMALSSKEANYVSGLVEAARTEHKEAQIEFFSPKSGSPLYCSGGKTSLFVYP
                     DGDVSICDLASQIKNNFHLGNVYNDSLKNCLSRSRTVNTNSIEACRQCAAREVCNGGC
                     RIISYSFYRNLDSPYPLCNMAGNNSSTANNSFLDFQEEKGYEE"
     gene            1570..2844
                     /locus_tag="PKL93_11040"
     CDS             1570..2844
                     /locus_tag="PKL93_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ71834.1"
                     /translation="MKFGLRTKNRNFTIYYFGRSVSLLGSGIQSLALSLYILDITGSG
                     RAMSTFLVISILPKIIFAPLAGVLGDRINRKSIMVNLDLLRGAIILFMCILTFNGHMG
                     ITYIYLFQLIISTIDIFFDPVTRSILPDIVKESDLIYANSIVNATDSLSYVIGPILGG
                     VLYPIGMGFVFLVNSVTFIASGISEIFIDYKKSSLNGKLTMEQVWKDFKNGLIALKRL
                     SGVLRMMSFITLTNLIMGPVVSLVVPFFAREVAKFDSYQYSIISSSWLVGITLGNIIL
                     SAIVGKLARKKIVLIGLLLQLIFFYIFCCLSFPWFVSFFGERSWAYILVLTTLFAAIG
                     FFNAFVNTPVGVYFQMAAPSEIRTRVLSVIALIAQISMPVGMALYGFVIDRFPAHLVI
                     LFSLILTTCITLFYSLTNSFELLESEQKYDNP"
     gene            2924..>3519
                     /locus_tag="PKL93_11045"
     CDS             2924..>3519
                     /locus_tag="PKL93_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ71835.1"
                     /translation="MRLPGDKLIWRTLRPILRGPFIKRYNLHAINSERIPDPPFLLVG
                     NHAYFIDAILIEAFVKYPVVWAVAAGNFKHPLAAPILKASGAIEKRKGIPDTAALRKV
                     LQVLKDGGIVGLMPEGSVTWDGEFGVVPPGTDKFLDRVDVPVVAARMNGGYLTRPRWA
                     DHHRLGWILRGIACKGFRSSWKGSSRSRSDSFRPHALET"
BASE COUNT          987 a          730 c          783 g         1019 t
ORIGIN      
        1 gcaatagtta tctggtaatc aattacctga gtacaacaat tcccgaactt tcaaacgagt
       61 atattcaatc aagttgtaaa aaaatactgt caagatctcc tggttattac atccatatct
      121 ggactttagg ggtaatttca tcatagaaat gcgtcagaat actcctatcg aagaataaag
      181 gagatagcat atggctgatt cggcgtatct tttatcacct gaagtacgat tgataagtac
      241 caacaaaggg ttttgggaga ttcgtttgga tagaaacctg caccgcttca tctcacaaac
      301 acagggttac atactgtcct tatgcgatgg aactaacact ttgaacgaca taatcaacca
      361 ggttgcaaga ctttatgaca agagttttga tgaatgctta gagaaggtaa gtggattcct
      421 acaggaagag gtcttaagcg gtgcagtaaa ctaccttgat agaaaactat ccgcaaaaat
      481 caacccctat gaagagatga aattcatgtt cgttccacag atggcagatg attttatagt
      541 ctctgaagaa ttagagagca taggcattat cctgacagaa agatgtgact acaactgtat
      601 ctattgcttt ggaagttatg gaagagcctc aacacaggca aaggaactta cactagacaa
      661 ggcaattagc ataattagag aggctcgtag cctctcggct gagcacataa tactctcagg
      721 aggagatcca tttgaatata gaggaatcct tgatattcta aatgttgtac gtttagaagg
      781 attcaggtct tgcgagatat cgtccaaagg tatcagattg accaaagaat tcgtaaaaga
      841 cttgaagaaa accggactga aaagaattca aattagcata gattcactca atgccgatag
      901 taatgactac ttatctggaa cagcgagatc attagaatat gcgttccgtg caatcttctg
      961 gtcaagatgg tatgggattg atgttagtgc aaggtctacc gtcactcgaa tgaactacaa
     1021 agaaataggg gcctttatag atagtctttg tcacatgggt ataaagcaaa taaggttagt
     1081 atctgtaatt ccggttggta aggcacttgc tgagatggct ctctcatcca aggaagcaaa
     1141 ctatgtttct ggactcgtcg aagcagccag aactgagcac aaggaagctc aaatcgaatt
     1201 tttctctcca aagtcagggt caccactata ttgctctggt ggaaagacat cattgttcgt
     1261 gtaccctgat ggagatgtta gtatatgtga tctcgcttct cagatcaaaa acaacttcca
     1321 tctaggaaat gtctacaatg actctctaaa gaattgtttg agtagatctc gcactgtcaa
     1381 tacgaacagt atagaagctt gccgacaatg tgctgcaaga gaggtttgca atggaggttg
     1441 ccgaattata tcttatagct tctatcgcaa tcttgatagt ccctatcctc tatgcaacat
     1501 ggcaggtaac aactccagca ctgctaataa tagttttctc gactttcaag aggaaaaggg
     1561 gtatgaggaa tgaagttcgg tcttcgcacc aagaacagaa acttcacgat ctactatttt
     1621 ggtagatctg tatctctcct cggaagcgga atacagtctc ttgccctttc gctgtacata
     1681 ttagacataa ctggctcggg tagggccatg agtacttttc tcgttataag catcttgccc
     1741 aaaattatct ttgcccctct tgcaggagtt cttggagaca ggatcaatag aaaatcgata
     1801 atggtaaatc ttgatttgct tcgcggcgct attattcttt tcatgtgcat tctgaccttt
     1861 aacggacata tggggatcac ttacatatat ttatttcagc tgataatttc cacaatagat
     1921 atattctttg atcctgttac aagatctatt cttccagaca tagttaaaga gagtgatcta
     1981 atctatgcga attctatcgt aaacgccaca gatagcctaa gttacgtaat tggaccgatt
     2041 ctgggcggag tcctctatcc catcggaatg gggtttgtct ttctggttaa ttcagtgact
     2101 ttcatagcat ccggtatttc tgaaatcttc atagactaca agaagagttc tctcaatggg
     2161 aaattgacca tggagcaagt ctggaaggac ttcaagaatg gactgatagc cctgaaaaga
     2221 ctatcaggtg tcttgaggat gatgtctttt ataacgctca cgaacctcat catgggtcct
     2281 gtggtttcgc tcgttgttcc tttttttgca agggaagttg ctaagttcga cagttatcaa
     2341 tatagtatta tcagttcttc gtggctcgta gggattactc tgggaaacat tatcctttca
     2401 gcaattgtgg gaaagctcgc aagaaagaaa atagtcctga tcggattgct tcttcaactc
     2461 atattcttct atattttctg ctgccttagc tttccgtggt ttgtaagttt cttcggagaa
     2521 aggtcgtggg catatattct ggtgctaacg acactgtttg ctgcaattgg attcttcaac
     2581 gcgtttgtca atactcctgt tggagtgtat ttccaaatgg cagcgccttc tgaaatcaga
     2641 acccgggttc tttcggtaat cgctttgatt gcccagataa gtatgcctgt agggatggct
     2701 ctttatggat ttgtaataga cagatttccc gcgcaccttg ttatactgtt ttctctgatt
     2761 ctcacaacct gcataactct cttctattcc ttgacgaatt ctttcgagct tcttgaatct
     2821 gagcaaaagt atgataaccc ataaaacatc gctaagcttt tcatcccgta gatgctggta
     2881 taataatcat attcttttcg gggaagtgag cgctttgcga aaagtgagat tgcccggcga
     2941 taagctgata tggcggacat tgagacctat tcttagaggc cctttcataa agcgctacaa
     3001 tcttcacgcc ataaactccg aaaggatccc cgatccgccc ttccttcttg tgggaaacca
     3061 cgcctatttc atagacgcta tactgatcga agcctttgtg aagtatccgg tcgtgtgggc
     3121 cgtggccgcc ggcaatttca agcaccctct ggccgccccg attctgaagg cgtcgggcgc
     3181 aatcgaaaag agaaagggca ttcccgatac agccgcgttg agaaaggttt tgcaggtctt
     3241 gaaggacgga ggaatcgtcg ggcttatgcc cgaaggctcc gtaacctggg acggtgagtt
     3301 cggtgtggtc cctcccggaa ccgacaagtt tctcgacaga gtcgatgtcc ccgtagtcgc
     3361 cgcgcgaatg aacgggggtt atctgaccag acccagatgg gccgatcacc accggctggg
     3421 ttggatactt cgtggaattg cttgcaaagg ttttcgaagc agctggaaag gaagttctcg
     3481 ctcaaggagc gattccttca gaccccatgc tcttgagac
//
LOCUS       DAOQYI010000104         3470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_28321_length_3470_cov_89.886384, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000104 DAOQYI010000000
VERSION     DAOQYI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28321_length_3470_cov_89.886384"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1814)
                     /gene="brxL"
                     /locus_tag="PKL93_11050"
     CDS             complement(<1..1814)
                     /gene="brxL"
                     /locus_tag="PKL93_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HNQ71836.1"
                     /translation="MELDALDLKVLKIYPEYVIKKSLVRNLKIGYNVPTYVLEHLLGT
                     YADLSDPRDIKGVESVREILEKHFLRPDMAELVKMQLRDKGFFRVIDRIDAWLDITKN
                     EYIAGLQNLSIKNGVITDELVKEHPKMLLGGMWAIIDLIYDHNTYPGRPFVVEKIHPI
                     QLSNFIRDQFVSRRKEFTVGEWTDLIIRSIGLEPKKMDDRQKLFQLLRLVPLVEKNYN
                     LVELGPRATGKSYIYREISPYSFLLSGGNTTVAQLFYNIATRKVGLVGEWDLIAFDEV
                     AGIQFKDKQALQMLKDFMESGTFARKVEVVAEAGIVFNGNINDDVQTLLKMSHLFEPF
                     PVEMQDTALLDRIHAYLPGWEVAKLRAELFTDHFGFAIDYFSEVMRSLRGSSAVNAPD
                     SYFNFGSQLNRRDEKAVRKTLSGLLKLLYPDGEYTKEAIEEVLAFSIECRRRVKEQLK
                     KLGGLEFWDTNFSYIDKESKKETYVSVNEGGSKALISQDPLPPGVVYTVSITGGKTTL
                     LKVETIVVPGSGRQNISGGGTKAKEALKTTISCIGSNQRKYLPSNSALKDYDIAVQLT
                     PMIGSEVGDDITVAIFVSILSAIHKNTLKKGLGIIGDMT"
     gene            complement(1830..>3470)
                     /locus_tag="PKL93_11055"
     CDS             complement(1830..>3470)
                     /locus_tag="PKL93_11055"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNQ71837.1"
                     /translation="EEASTLIAEKILEASYKSRPGDELHEYYSEWTRESEWEESLHLH
                     AEKFSKVRKEELLSNIELFEDDYKHPFTVIEKELFDRRIQAILQEEDAAQAIEFAKER
                     ARKRKKRDEDREAGVYWEELLWLEPLLQEPDLSSIGSLESFLSVYSNTLWKYDSLDRK
                     LRHSHLPDKLKTWAVKKVSGINKIAENHWKSHYDPKIQTEQPGLLYRILKGEGKKAVI
                     VVDALRYELSCELSLKRKANIQNKPILAVTPTETPVGMGALFSSGEIEKILKDKRVLI
                     VDKKTGKTLDSVPNREENLKELLPGVEIIQLGSELPETEKLVVKTRDIDSMGHEELME
                     FYGDIMTKLSDLADKLVKAGYEVHFTSDHGFYLPVPGETVKQDKTVSYSSGIRYSLNS
                     AKPEEGKYEQTGSSYILYANSGNVFKDYGGHFWHGGITYQEVIIPHLVITPVVGERRK
                     RVMIGNKDRLKVVQKDHFEVFLFTEIDMFGIAPRVYIQCLDQKIEIEEPVSEETRQTI
                     KVNAKSGETFKIEVRDLEDGTLMDWVECEYLPTRERIF"
BASE COUNT          666 a          940 c          802 g         1062 t
ORIGIN      
        1 gtcatatctc ctattattcc caatcccttc ttcagggtgt ttttgtgtat ggccgagagt
       61 atagagacga atatggcgac cgttatgtca tcgcccactt cgctgcctat cataggggtg
      121 agctgcaccg ctatgtcgta atccttcaac gctgagttgg atggcaggta ctttcgctgg
      181 ttagagccta tgcaggagat cgtggtcttc aaagcttcct tcgcttttgt cccgccgccg
      241 cttatgttct gtcttcccga accgggaaca actatagttt cgaccttcag gagtgtcgtc
      301 tttccgccgg ttatcgagac tgtatagacc acgcccgggg gcagtggatc ctgcgagatc
      361 agggccttgc ttcctccctc gttcacggat acataggtct ccttcttgct ctccttatct
      421 atgtacgaga agttcgtgtc ccagaactcg agccctccca gtttcttcag ctgctccttg
      481 actctcctcc tgcattctat ggagaaggcc aggacttctt ctatcgcttc tttcgtgtac
      541 tctccgtccg gataaagaag tttcaacaaa ccggatagag tctttcggac cgccttctcg
      601 tccctcctgt tcagctggct tccgaagttg aagtaactgt ccggtgcgtt gactgcggaa
      661 cttcccctta aggacctcat aacctcggag aagtagtcta ttgcgaaacc gaagtgatct
      721 gtgaagagtt cggctctgag tttcgccact tcccagcccg gaagatatgc gtgtattctg
      781 tcgagcaggg ccgtgtcttg catctctacg gggaagggct caaatagatg agacatcttc
      841 aacagagtct gcacatcatc gttaatgttt ccgttgaaga ctatgcccgc ctcggcaacc
      901 acctctacct ttctagcgaa agtcccagat tccataaagt ctttcagcat ctgaagcgcc
      961 tgtttgtctt tgaactgtat cccggcaact tcgtcgaagg ctatcagatc ccactcccct
     1021 acgagtccga cctttcgtgt ggcgatattg tagaaaagct gggcgacggt agtgtttccc
     1081 ccggatagga ggaatgaata aggcgagatt tctctgtaga tatacgactt accggtagcc
     1141 ctcggtccca gttccacgag gttgtagttc ttttcgacca gagggacgag tctgagaagc
     1201 tggaagagct tctgtctgtc gtccatcttc ttcggttcga gacctatcga gcggattatg
     1261 agatcggtcc actcgcccac cgtgaactct tttcttctac tcacaaattg atctcgtatg
     1321 aagttgctca actgtatggg atgtatcttc tctactacga agggtcttcc ggggtaggtg
     1381 ttgtggtcgt agatcaaatc tattatcgcc cacataccac cgagaagcat tttaggatgc
     1441 tccttcacca gttcatccgt aattaccccg ttctttatgc tcagattctg cagtccggcg
     1501 atgtattcgt tcttggttat gtccaaccag gcgtctatcc tgtctatgac acgaaagaag
     1561 cctttatcgc ggagctgcat cttcacgagt tccgccatat ccggcctgag aaagtgtttc
     1621 tccagtatct ccctgacgct ctccacgccc tttatgtctc tgggatcgga caggtcggcg
     1681 taggttccta gcaggtgctc gaggacgtaa gtcgggacgt tgtagcctat cttcaagttc
     1741 ctgacgagcg acttttttat cacgtactcg ggatatatct tcagaacctt caggtctagg
     1801 gcgtccagct ccactcttct ctcccccaat cagaatatgc gctctctagt tggcaaatac
     1861 tcacactcca cccagtccat aagagtgccg tcttccaaat ctctcacttc tatcttgaag
     1921 gtttcgccgc tcttcgcgtt cacctttatt gtctgtctcg tttcctcgct caccggttct
     1981 tctatctcga tcttttgatc caggcactgt atatacaccc tgggagcgat accgaacata
     2041 tctatttcgg tgaagaggaa gacttcgaag tgatccttct gaaccacttt cagtctatct
     2101 ttatttccga tcatcactct ctttcttctc tcccccacaa caggggttat gactaggtga
     2161 gggattatta cctcctggta agttattcct ccgtgccaga agtggccgcc gtagtctttg
     2221 aatacgttcc cgctgttggc gtagagtatg tagctgctgc cggtctgttc gtatttgcct
     2281 tcctcgggtt tggcgctgtt taggctgtac ctgattccgg atgagtagct tacagtcttg
     2341 tcctgtttga ccgtctctcc ggggacggga aggtagaagc cgtggtcact cgtgaagtgc
     2401 acctcatagc ccgccttaac cagtttgtct gccagatcgg agagtttggt cattatgtct
     2461 ccatagaatt ccatcagctc ttcgtggccc atagagtcta tgtctcgagt ctttacaacc
     2521 agcttttctg tctcgggcag ttccgagcct agctggatta tctccactcc cggaaggagt
     2581 tccttcaggt tttcctctct attcggaacg ctatccagcg tcttgccggt cttcttatcc
     2641 actatgagga ctcttttgtc cttcagtatc ttttcgatct ctccggatga gaagagtgct
     2701 cccattccca cgggcgtttc cgttggcgtt accgcgagta tgggtttgtt ttgtatgttc
     2761 gcctttctct taagactaag ctcacaggag agttcgtatc tcagcgcatc taccacgata
     2821 actgccttct tgccctcacc cttcagtatt ctgtaaagga ggccgggctg ttccgtttga
     2881 atcttaggat cgtaatggct tttccagtgg ttctccgcta tcttgtttat cccggatact
     2941 tttttcacag cccaggtttt cagtttgtcg ggcaggtggg agtgtctcag tttcctgtcg
     3001 agagagtcgt atttccagag agtgttcgag taaacagaga gaaagctctc cagagagcct
     3061 atgctcgaga ggtcgggctc ttgcaggagc ggttctagcc agagtagttc ctcccagtag
     3121 actcccgctt ctctgtcctc gtctctcttc tttctctttc tcgccctctc tttcgcgaac
     3181 tctatcgcct gtgcggcatc ttcttcctgt aagatggcct gtattcttct atcgaatagc
     3241 tctttctcaa taaccgtgaa tggatgcttg taatcatcct cgaagagctc aatgttggac
     3301 agcagttctt ccttgcggac cttggagaat ttctctgcgt ggaggtgaag gctctcttcc
     3361 cactcgctct ctctcgtcca ttcactgtag tattcgtgaa gttcatcgcc cggtctgctc
     3421 ttgtatgagg cctcgagtat cttctcggct ataagggtgg aggcttcctc
//
LOCUS       DAOQYI010000105         3342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_29938_length_3342_cov_55.940067, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000105 DAOQYI010000000
VERSION     DAOQYI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29938_length_3342_cov_55.940067"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(716..985)
                     /locus_tag="PKL93_11060"
     CDS             complement(716..985)
                     /locus_tag="PKL93_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71838.1"
                     /translation="MKSEEISYEILQIRDFGKAMRRRNVKIRIFNYSQSEDSLKKLAE
                     SIWLQHGQDVEELTTVFYLPGMNPKSTGFALGGCMKGKGCYVTLD"
     gene            1181..2428
                     /locus_tag="PKL93_11065"
     CDS             1181..2428
                     /locus_tag="PKL93_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNQ71839.1"
                     /translation="MRGFDELRYIRPTLVIFIFLVTILLMMLPYHVFQNEAISYTQEI
                     YSGIFETSVENLGSALSLGYFQWNAMYDAVLSKDDLFIEEMLQEIQNEFPAVKSARLV
                     SRPPEIPAKDVYKLATIEQEVYLYFNVYDSHMDNEISDAVVIVSIDKNVLLSEMSLDD
                     RITFSPGGSKSFAYGLRTEIKGYDLTKWMILHSVAAGIVGVLFMMEALALGLSRYYEM
                     SGLQEIMYLWEMEDSYTAFHSKNVAKIAEFLGKSLGLRRKKVMELVNGAKLHDIGKMG
                     ISPNILRKHGPLDEIEKEVIHNHPQHGKEVLQHFKYLERFIPYAYLHHEREDGSGYPQ
                     GLKGDEIPLEAKILAVADVFEALTADRPYRTAYSFGQAVDMMTEMPLDQDIVSELISI
                     LPELENELCGRNPRKCGIIRSQS"
BASE COUNT          951 a          717 c          734 g          940 t
ORIGIN      
        1 gtcaccgggc ttctttccaa tcttggctct tatatccttc cttatgacga ttacgtagca
       61 gaccgtacca ccaggttctt cattcccata ttaacaacgc ttccgccgta cggttagccg
      121 tcaaaactcg cgcggaccct taatcctgcc cctgccaaat tccttcctta aatcattagg
      181 gaattcgcta aatgccccat ctatgtcggg agcttcgaaa agatggcatc agactcatat
      241 actttcgggt tcttagtcaa taactccctt cagaaaagat cttcagtagc ctctccgcgg
      301 cgaaaataaa caagtacgtt atttcacggc tcttatcaca gagcgcctcc atcaatgttc
      361 aggctcataa tcctgtaatt ctttgtcccg atcgctgaat ctgaatctaa tagtcaaacc
      421 gagaacaaat caaactgaat atgtatatag ataatacacg gttgctctta tgttgtgaag
      481 aagcgatcgt tctctatggc agtcaagaaa ctcatcgaga aaagtgcgat aattctaggg
      541 ttttatcttt caaaaaagaa atcggaaatc cgatttcaag cagcaacaaa agtcgttatg
      601 tgaaaccagt attcaatgaa ctgagcttta acctgagctg catggtagat agaaaagtcc
      661 cgctttcgac cgtactctaa ctcatccatt tatcccgggc cgagcgttcc tagagttagt
      721 ccagtgtaac gtagcatccc tttcccttca tgcagcctcc tagcgcgaat cctgttgatt
      781 ttggattcat tccaggcagg tagaaaacgg ttgtgagttc ctcaacatcc tggccatgtt
      841 ggagccagat gctttctgcc agtttcttca ggctgtcttc ggactggctg taattgaaga
      901 tccgtatttt gacgtttcta cgtcgcattg cctttccgaa atccctaatt tgaaggattt
      961 cgtagctaat ctcctcgctt ttcactgttt atccccctaa gaataattac aacacggata
     1021 ttattttaca gcagttaaca ccaaaagcaa aatctccaat ttaattgtct ttacagtagt
     1081 agttcgccaa attactatca agacattgtt gaatcaatag attaagtgat atgattaagt
     1141 agatattttg aggggtggat ataacagaag gaggttttaa atgagaggat ttgatgaact
     1201 ccggtacatc agacctactt tagtcatatt catctttcta gtaacgattc ttctaatgat
     1261 gctaccgtac catgtctttc agaatgaagc tatcagctat actcaggaga tctattcggg
     1321 aatctttgag acatctgtcg aaaatctggg atccgctttg tctttgggat atttccaatg
     1381 gaatgccatg tacgatgccg ttttgtcgaa agacgactta ttcatcgaag agatgctcca
     1441 ggagatccaa aatgagtttc cagcagtgaa gtcggccagg ctagtaagca gaccgccaga
     1501 gataccggct aaagacgtct acaaactagc aaccatagag caggaggtct atctctactt
     1561 caacgtttac gacagtcata tggataatga gatctcagat gcagtagtga ttgttagtat
     1621 tgacaagaat gttctgcttt cggagatgag tttggatgat agaattacct tctctccggg
     1681 cggatcgaag agtttcgcct acggcctgag aaccgaaata aaaggatatg accttactaa
     1741 gtggatgatt ctgcattccg tggcggctgg gatagttggt gtcttgttca tgatggaagc
     1801 gctggcgctc ggtctgtcta gatactatga gatgagtggt cttcaggaaa ttatgtattt
     1861 gtgggagatg gaagattcct acacagcctt tcactccaaa aatgtggcaa aaattgctga
     1921 gtttttgggc aagagtcttg gattgagaag aaagaaagtc atggagcttg tgaacggagc
     1981 aaaacttcat gatattggta agatgggtat ttcaccaaac atactgagaa agcatggccc
     2041 tctcgatgaa attgaaaagg aggtaataca caatcaccct caacacggta aagaggttct
     2101 tcaacatttc aaatacctcg agagattcat tccttacgcc tatctacacc acgaaagaga
     2161 agacggttct ggctatcccc agggcctaaa gggagatgag ataccgctcg aagcaaagat
     2221 cttggccgta gctgatgtgt ttgaagctct cacagcggat aggccctaca ggactgctta
     2281 ctcttttggt caagcggtgg acatgatgac agaaatgcct ctcgatcaag atatagtatc
     2341 cgaactgata agtattcttc cggaactcga gaacgagctt tgcgggagga atccgcgtaa
     2401 gtgcgggatc attagaagtc aaagttgatt ttctggatag cctcatcaaa caagtctgtg
     2461 acgcaagcaa ctcatacact cttctgcttc tggtcggagc gatatgatgg gcgttgattt
     2521 cgttgtgcgg gttttctgtc tagaaggctt agcagatcga cactttctga agattgactc
     2581 aatacaaagg agaagcatag aataatagac aggggtggtg aaaatcacag tttcaagcgt
     2641 tatcccgatt ttggtcgtag ggcttgttgt cacggtaata gccatattcg ttcgtggttt
     2701 tacgatagtc atggacaata tctagatgcc ggagccattc tccaaatttg gatttctttc
     2761 gcagtgatgg actgtaaaaa aactttcgat gatgcacttt cttgtctcca tcgcttcttc
     2821 atttgaagga aattggcagt atagtattga ttgtgatttt ctggtgaaga acatgagtca
     2881 taccttctgc aaatacattc ctgcagaaac aaaagcccat gaagacccgt ccccccatct
     2941 tactcgaata gtcctcagga tgagaaatag agtacatagg ctctcattgg caactcttca
     3001 gcaacagtaa gtgaatacat ctacagtcta tatttcgcga agtcacttct cattatgcag
     3061 ggaaatctaa aacgggttgg ttattttcag ctcagtgcat cgccatgaga gcaaaaatcg
     3121 gatgtttagt gaaaactgct gatcacagcc actggcaatt ctaaaagcct tagagctaat
     3181 gaaacagtgg tcttggatcg ccgaaaaact cgcccaaaaa caaacaagcc tcccaacggg
     3241 aggctagatt cattgatctg agcgcttcat acacgcacgt aggccctaaa tgcttggtgg
     3301 cggcggtcag aatcgaactg acgactctgc gggtatgaac cg
//
LOCUS       DAOQYI010000106         3282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_30751_length_3282_cov_72.853114, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000106 DAOQYI010000000
VERSION     DAOQYI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30751_length_3282_cov_72.853114"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..394)
                     /locus_tag="PKL93_11070"
     CDS             complement(197..394)
                     /locus_tag="PKL93_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HNQ71840.1"
                     /translation="MQCEVDRNLEKCNCTYPGCPRKGKCCECIAYHRRNGELPACYFT
                     EEQEKTWDRSIEYFVKVNSKR"
     gene            554..880
                     /locus_tag="PKL93_11075"
     CDS             554..880
                     /locus_tag="PKL93_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71841.1"
                     /translation="MRKVLLILLFVSMIFGLTLANNGKGPGDGIPDGQEPGAGFGSPG
                     TGKGEGPGAGKVDSGGSTTLLLENLEAETASSEPVLVRSMNRLVEMIKNMLRNLFRVR
                     TALSAK"
     gene            1182..1394
                     /locus_tag="PKL93_11080"
     CDS             1182..1394
                     /locus_tag="PKL93_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71842.1"
                     /translation="MKRITAIVLFLLLAFGIALSINGSGPGDGVPDGPDDDAGYGAPD
                     DSCEGNGPGQGKPALDEDEIVIGEIS"
     gene            2274..2519
                     /locus_tag="PKL93_11085"
     CDS             2274..2519
                     /locus_tag="PKL93_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71843.1"
                     /translation="MKPASIRKQMIDELENIPEDKLQLLLELIRSSKSDLSDLQPKRK
                     LITDYAGSWKEIDSDQYNTMIKELRDRREKSRRERNV"
     gene            2512..2661
                     /locus_tag="PKL93_11090"
     CDS             2512..2661
                     /locus_tag="PKL93_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71844.1"
                     /translation="MSDLALVDTDILSMFFRGHSPVVSRMEEYATYHEKVYLSIITYT
                     KYSAV"
     gene            2658..2918
                     /locus_tag="PKL93_11095"
     CDS             2658..2918
                     /locus_tag="PKL93_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNQ71845.1"
                     /translation="MRHIEATRQIEQFLEFRLFNEVLPITLESASIAATHYAYLRRLG
                     TPLDDMDLLIAGIAIENDMTLVTHNMKHFSRIPGLKLQDWIE"
BASE COUNT          910 a          746 c          744 g          882 t
ORIGIN      
        1 gaagagaatt tggtaacact ccttaaaagt gaagaaccgc ttatagaact tctgattcaa
       61 ctcttcctcc agaagaatct cgatcccgaa tcgagattag gcaatttata atcattccat
      121 tttagcatct aatgctgaat ctcatttgga agaggatata cattccactt gttggagatt
      181 tcattatatc tgaatgctat cttttgctgt tgaccttcac aaagtactca atgcttctgt
      241 cccaggtttt ctcctgttct tcagtgaagt aacaggccgg aagttctccg tttctacggt
      301 gataggcgat gcactcgcag cactttccct ttcttggaca gcccggatag gtgcagttgc
      361 atttctcaag atttctgtcg acttcacatt gcaagatcag tacctccctt tgttcatttc
      421 attcctgatt ttatcagagg aatcctcctc aaccgattct gcatagccgg cttcccagaa
      481 gattcttcac cattaatctc tctttaatca agaatgctct aacatactaa tatatgagaa
      541 ggggggaacc gtaatgagaa aagttctact gattttactg ttcgtctcca tgatatttgg
      601 cttgaccctg gcaaataacg gcaagggtcc cggtgatggt attcctgatg ggcaggagcc
      661 cggggcaggt tttggttctc caggaacagg aaagggagaa ggtcccggcg ctggtaaagt
      721 cgactctgga ggcagcacga cattgctgct ggaaaacctg gaggctgaaa ccgcgagttc
      781 tgagcctgtt cttgtccgct ccatgaatag attggtcgag atgatcaaaa acatgttgag
      841 aaatcttttt agagtgcgta ccgctctctc agcaaaataa aaaccaatta cccctgttta
      901 ctcctagact aaaccgccgc taccgtatta ccaggacagc ggcggccttt tcttttatgt
      961 gtagtcacta tagagcgcag gcaaattagg gtgcgtcaac ccccccccgg gagagagaat
     1021 ccgctttaag ataagagtgg agtcgtttca aaatctgcct tgtcttcctg aaacttccgt
     1081 tatcgagacg gtcagacagt tcaattagcg aagtctttaa tcactcttta atgaggtaag
     1141 aaataggatc taatcatttg aatacaacgg gggtgatgaa aatgaaacga attaccgcaa
     1201 ttgtgctttt cttgttactt gcctttggaa tcgctctttc aatcaatggc agcggtccgg
     1261 gcgacggtgt gcccgatggc cctgatgacg atgcgggtta tggagcacca gacgattcct
     1321 gcgagggaaa cggtcccggt caagggaaac cggcactcga tgaagatgag atcgttattg
     1381 gagagatttc ataaaagggt acatttctct gaattttgaa gcttgagggg gaccgaagaa
     1441 agtcggcccc tctaaacttt ctatatgagc atttttcatc gacaataaat gccttcggaa
     1501 attccattac tctatcttta gaaaaggaag gaaacagcca acaataagtc gcaaatcaca
     1561 gggcatccta attctctagt ctaaaagaat tatcttaggg caattcttgc acttgtaact
     1621 ggaactgaca actgttctaa gagccgtggt tggtggtacg cggttggtgg ttggttggag
     1681 ctaaaggaag agaagacagc aatcaccccg cggaagaata tcaacttgtt tgaaccaact
     1741 caatttgcca tcctaatgtg atcttgatca ggatctaatc tttgatctta taggtgaacg
     1801 gattcatgat ctgggacggt caacggtgaa cagctcctcg cagcgatcag aggctctcag
     1861 gatcgagatg ctgaaacaag ttcagatgac gaattattac gctttcggaa caagcattcg
     1921 ttgtcattcc acggagcctg tcctgaagcc tgccccaaaa tgctcttgtt tggggtctct
     1981 gttcagggct cctgtttggg gtctcctgtt gggctcctgt tggagcctct gttaaaggtc
     2041 atcccgaact cgatcccgga tctggtcttg aaaacacgtc aaggatcgag atcctggcca
     2101 gaagctcgcc agcatgacgt taagcggtgt cattcgccaa ttcctacgta ttgtcatccc
     2161 gcagtgttcc tgtgcgggat ctcgctcttg tcatccgtct cgccctttcg atatgaatgc
     2221 tttgctccaa acacgtgtta aaataatacc gagaattctt gggaagtgat cttatgaaac
     2281 cggctagcat aaggaagcaa atgattgacg agcttgagaa tatcccggaa gataagcttc
     2341 aactgctact ggaactaatc agaagcagta agtccgatct ttcggatctt cagccaaaga
     2401 gaaaattgat taccgactat gcaggttcat ggaaagagat cgattcggat caatacaaca
     2461 ctatgatcaa agaactaaga gaccgaagag aaaagtcaag aagggagcgc aatgtctgac
     2521 ttagcacttg tggatacaga catactttcc atgttcttcc gcggccattc tccagtagta
     2581 tccagaatgg aagaatatgc aacatatcac gagaaagtct atctgagcat aataacgtat
     2641 acgaaatact cagcggtttg agacacattg aagcgacaag acaaatagag caattcctgg
     2701 agtttcgtct tttcaatgaa gtacttccta ttacgcttga atcagcttct attgctgcga
     2761 ctcactacgc gtatcttaga agacttggaa cgcctttgga tgacatggat cttctcattg
     2821 ccggtatagc catagaaaac gacatgactt tagtcacgca caatatgaaa cacttcagca
     2881 gaattccggg attgaagctg caagactgga tagaataact gaatccccga tattgttcag
     2941 cgaccctccc acttgatgtc ccccatgtca ttgtgaatag ttagcacaga atatcgtcag
     3001 agaagcgcta cacgcctcaa agaaatgtcc tttgtcagcc atccgagaga aagggagtcc
     3061 tacgtccgat gggatcaatc gagaaagagc acattcgaca atccgctccg ctcactgcag
     3121 taaaagaatt aacatttgtc gaccgacgaa ggttctccgt tctccgcaaa aagcaaaata
     3181 cttttgaagc atcacctaat gaagtcatcc cgaagagcct gccttgaagc ctgccctgaa
     3241 gcctgcccca aaatgctctt gtttggggtc tctgttcagg gg
//
LOCUS       DAOQYI010000107         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_32376_length_3167_cov_79.581620, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000107 DAOQYI010000000
VERSION     DAOQYI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32376_length_3167_cov_79.581620"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1194)
                     /locus_tag="PKL93_11100"
     CDS             complement(292..1194)
                     /locus_tag="PKL93_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ71846.1"
                     /translation="MLFTFQDGDSPMGGETSVYLIEGGDRFYLCDTFLGSRFMNVVKD
                     RIKESPRKDLVIFNSHSDYDHVWGNGAFDGCEIVAHELARRRMEERWEYDFENLERFR
                     DGDVLKRLPNVTFSDRLVFEDDEIEFRYLPGHTLCSSVCIDRRDSVIFVGDLVEDPLP
                     LTLWSDLVTFTESLKTVLKMSCQTVISGHSGIVAESLIRRNLEYLKSLFLGEEIDVEE
                     GEETHSFNTKYLEFLHYEDKAREILGERFDFVQFMREFWQMLGVERESLRFESRLIRQ
                     TPSERLEEIWPQFEEQLKNRKDFE"
     gene            complement(1221..1856)
                     /locus_tag="PKL93_11105"
     CDS             complement(1221..1856)
                     /locus_tag="PKL93_11105"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNQ71847.1"
                     /translation="MTRYVDLTRVIEDNQPVYPGDDQTRLHRSRVLSKDGYNNHRLEI
                     SMHSGTHIDGPMHLTQSNLYVDQIEIDRLIGKGILLDVRGETEITIKPQYEEAISEDS
                     VVLFWTGRDSLFGSEDYFLKNPYLTSDLAEFLVKRKTRMVGFDSSSPDRYPYDIHRIL
                     FRGGCFIAENLTGLENLSDSKELEIFAIPLKIHADSSIARIFAAVKQGGDP"
     gene            2038..2415
                     /locus_tag="PKL93_11110"
     CDS             2038..2415
                     /locus_tag="PKL93_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71848.1"
                     /translation="MRRILAYLLVLLFVVSSASSLIGSSLDVAKEIAESANERIESLI
                     ETAMEKAESLTERFEETGLSLEAYENLIDDLGNDLVNTALRISQRAIEKIEEIGYNAI
                     CYYVPVKLGYKIFMVDPILIIDD"
     gene            2555..>3167
                     /locus_tag="PKL93_11115"
     CDS             2555..>3167
                     /locus_tag="PKL93_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ71849.1"
                     /translation="MEGFKVCKSSETIETVAQKNSSLSLLAHGDGVEISMYELRSGVV
                     TFLDTFSGGDLLEFFYIIEGTISCNYSKKEVNLGPGDYFYAHNLKSPVELKPQTDVRL
                     LHVSSRPLFKYVSNNMGVMKDILSKVEIKDSYTHGHGKRVRDVSLAIARKLGVPSEKQ
                     EVIAMGALFHDIGKIEIDDSILKKPSRLSREEFEIIKLHPVTGC"
BASE COUNT          894 a          718 c          658 g          897 t
ORIGIN      
        1 gctgcccttc aatcgtttct tgcgttagag gggcagcagt ttaagatgga agagcaatga
       61 tgcccgaaaa taacatttca tgccaattaa tcaaaaagaa atttcacttg accgtgataa
      121 ggaaaataag atctatgttg gcttatgaac ctctgtctct cgtttcacct tgcaatcgtg
      181 aagtacataa tcagtcccac aaccaccgtg ataccgataa tcaaaggaat aatcgcaaaa
      241 ccaaacatcc tctcacctcc atctaagagt tgctcaatat tctactctct ttcattcgaa
      301 atccttcctg ttcttcaact gctcttcaaa ttgcggccag atctcttcaa gtctttcaga
      361 aggagtctgt cggatcaatc tcgattcaaa tctcagagat tccctttcca ctccgagcat
      421 ctgccaaaac tctctcatga actgcacgaa gtcaaacctc tcgccaagaa tttctcttgc
      481 tttgtcttca taatgcagaa actcgagata ctttgtgttg aatgaatgtg tctcttcgcc
      541 ttcttcaaca tctatttctt caccaaggaa aagagatttc agatactcca gattccttct
      601 tataagactc tcagcgacga ttccagagtg tccagaaatt acggtctgac acgacatctt
      661 aagtacagtc ttcaaactct ccgtgaaggt taccagatcg ctccagagag ttagtggtaa
      721 aggatcttcc acaagatctc ctacgaagat caccgaatct cttctgtcga tgcatacaga
      781 agaacagaga gtgtgccccg gcaaataacg aaactctatt tcgtcatctt caaagactaa
      841 cctatcgctg aaggtgacat tgggaagtct cttcaaaaca tctccgtctc gaaatctttc
      901 cagattttcg aaatcgtact cccatctttc ttccattctt cttcttgcaa gttcatgagc
      961 gacgatttcg cacccgtcga acgctccgtt tccccagacg tggtcgtagt cagaatgtga
     1021 gttgaagatt acgagatcct ttctggggct ttctttaatt cgatccttaa caacgttcat
     1081 aaaccgactt cctagaaacg tgtcacacaa ataaaagcga tctccgcctt ctatcaaata
     1141 gacggacgtt tccccaccca tcggtgaatc tccgtcttga aaggtaaaca acactcccct
     1201 tctgccgact gtccgaatcc tcaagggtct cctccctgtt tgaccgcggc aaagattcgg
     1261 gctatagagg aatcagcatg gatcttcaac ggaatcgcga agatttccag ttctttcgaa
     1321 tccgacaggt tttcaagacc ggtaagattc tctgcgatga agcagcctcc acggaaaaga
     1381 attctgtgga tatcgtaagg gtatcgatcc ggagaagatg agtcgaaacc aaccattctc
     1441 gttttacgct ttaccagaaa ttcggccaga tcggaagtca ggtatgggtt cttcaagaaa
     1501 tagtcctcgc taccaaacag ggaatccctt ccagtccaga aaagaacaac cgagtcttcg
     1561 gagatcgctt cttcatattg cggcttaatt gttatctctg tttcgcccct cacatctagg
     1621 agaattccct tgcctataag tctatcgatc tcgatctgat ctacatagag atttgattgg
     1681 gtgagatgca tgggaccgtc tatatgggtt cccgagtgca tacttatttc aagtctgtgg
     1741 ttgttgtatc cgtctttgct caagactctc gacctgtgga gcctcgtctg atcatcacct
     1801 ggatagaccg gctgattgtc ttcgattact cttgtaagat cgacatatct agtcaaagca
     1861 tcacctcgct attattatac gaaaatacaa caactacaga atatcacagc aaaggcctta
     1921 cagagcgaaa ctaatgaaag gtagtttttc attaatgaag ggaagatctt catttgaaat
     1981 caagagatta tatgatatga ttgatacgtt cgaaagaaat tgaaggagga gatttgtatg
     2041 agaagaattc ttgcttatct attagtgctc ctcttcgttg tgtcgtctgc atcgtctcta
     2101 atcggttctt cattggacgt agcgaaggag atagcggaat cggcaaacga gcggatcgaa
     2161 tcgctaattg agaccgccat ggagaaagca gagtccttga cagagcgttt tgaagaaacc
     2221 ggtctttctc tggaggccta tgagaatctg atcgacgatc tggggaacga tctagtgaac
     2281 acggctctac gcatttcaca aagagccatt gagaaaatcg aagagatcgg ttacaatgct
     2341 atttgttact atgtgccggt aaaacttggt tacaagatct ttatggtcga tcccattctg
     2401 ataatcgacg actagaagag ccaaataaac taattgtgcc agaaatgccg tagtcatcta
     2461 cggcatttct gattagctaa aaccttcaga gtatatgtga tacactgtat ttgagcatca
     2521 acttattcta taggctttat ttccggggga agagatggaa ggtttcaaag tttgcaagtc
     2581 aagcgaaacc atcgaaacgg ttgcccagaa gaattcttca ctctctctgc tggctcacgg
     2641 cgatggcgta gagatttcga tgtatgaatt gcgctccgga gttgtcacat ttctcgatac
     2701 attttcgggc ggggacctcc tcgaattctt ctacatcata gagggaacta tttcctgcaa
     2761 ttactccaaa aaagaagtca atttaggtcc cggagattac ttttatgccc ataatctcaa
     2821 gtctcctgtc gaactgaagc cccagactga cgttaggttg cttcatgtat cttcaaggcc
     2881 gctgttcaaa tacgtaagca ataatatggg ggtaatgaag gatattcttt ccaaagtgga
     2941 gattaaggac tcgtacactc atggccacgg aaagagagtg agagatgttt ctctggcgat
     3001 tgcaagaaaa cttggtgttc cttcagagaa acaggaagtt attgcgatgg gagctttgtt
     3061 ccatgacatt ggaaagattg aaatagacga ttcgatattg aagaaaccct ctcgattgag
     3121 tagggaggag ttcgagataa tcaagcttca tccagttact ggttgcg
//
LOCUS       DAOQYI010000108         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_32569_length_3156_cov_69.808449, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000108 DAOQYI010000000
VERSION     DAOQYI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32569_length_3156_cov_69.808449"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /gene="iscB"
                     /locus_tag="PKL93_11120"
     CDS             complement(<1..886)
                     /gene="iscB"
                     /locus_tag="PKL93_11120"
                     /inference="COORDINATES: protein motif:HMM:NF040563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HNQ71850.1"
                     /translation="MLVYVISKHGKPLMPCNPQKARRLLKEQKAKVVKRTPFTIQLLY
                     GSSGYKQDVILGVDAKSKTIGVSASTENKELFSAEVELRTDIVDLLSTRRTLRRSRRN
                     RKTRYGQSRFLNRRKPEGWVAPSVQNKIDTHIKVVKLVHAILPITRVVVEVAQFDVQK
                     IKNPDILGEDYQQGEQLGFYNVREYVLFRDKHTCQHCNGKSRDPILNVHHIESRKTGG
                     NSPDNLITLCETCHKRYHKGGIELKVKRNSSFRDAAFMGIMRWASYNKLKEQYSNVHL
                     TFGYITKNVRIKHNLEKSH"
     gene            complement(1084..2712)
                     /locus_tag="PKL93_11125"
     CDS             complement(1084..2712)
                     /locus_tag="PKL93_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ71851.1"
                     /translation="MNRKIPSLLNGEFGIFYSRPIVNMYMDKLPTSEWREFIKVLDCM
                     YAGEDSSAIVSMIEHGLNRSTRNRSLFYLLLSIKLLVSNGENDELYNKLRREFGRIPS
                     GVRKEVAWALTNYYGLYHPEREIRVFRVWSEVYEKESSARILLLVGRARRAAQRGNRD
                     QARDLFEEALKMAKKLEDPQGLINVLNDYSWYMKQDNIEYSLKLAERAAYYSGYYDEK
                     LSSASCVLDTLSIIQHESKDKGLYKTIELQRMTGFEKDGPLQTLRDRLAVVELEQSEY
                     PNEDLTRSYLKKHAKNVKNTSRKTCVAWDNLSYLLNGRTRRIRGVTLRKIIIGLEIAV
                     DLLNDPFPIVNEYVKSKVEESFEIAINELGKLSSYNAKKLVLSTYSALRDRMGSFPYL
                     CRKGVLLRIVEACSIDLHTLKEIAKRRYELMKFIGQLFDLHPFLEGRRDAAMKFLDVM
                     PKRKSEEFISGYLQLTEKERVIIDTFMRDYARYNRDWGVRLEPISELKLFVQEYGLKN
                     VPSTLAYWVLDTKRCRTKLVNLLVKYPTAKAVSL"
BASE COUNT          848 a          713 c          582 g         1013 t
ORIGIN      
        1 ggtgtgattt ttcgagattg tgtttgattc gaacattttt ggttatatag ccaaaagtca
       61 aatgcacatt tgaatactgt tcttttagtt tgttatagga agcccatcgc attatgccca
      121 taaaggctgc atccctgaat gaagagttgc gcttgacttt aagttcgatc ccccctttat
      181 ggtatctctt atgacaggtt tcgcatagag taatcaaatt atccggagag ttcccgcctg
      241 tctttctcga ctcgatgtgg tgcacattta gaattggatc tcttgactta ccattacagt
      301 gttgacaggt atgtttatcc ctgaacaaaa catactccct gacattatag aatcctagct
      361 gttcgccttg ctggtaatcc tcgccaagaa tgtcggggtt ctttattttc tgtacgtcga
      421 attgagccac ttctactacc actctagtta ttggcaggat tgcgtgaacc agcttaacca
      481 ctttaatgtg agtgtcaatc ttattctgta ccgacggtgc tacccaacct tcaggctttc
      541 ttcgattaag gaaacgagat tgtccataac gagtctttct gtttcttctg gaccttctaa
      601 gcgtccttct ggtagataac agatctacaa tatctgttct caattcgact tctgccgaaa
      661 acagctcctt gttctcagtt gaggccgata ctcctattgt cttgcttttg gcatctacac
      721 cgagaattac atcttgtttg tatccagagg aaccgtacaa cagttgaata gtaaagggtg
      781 ttctttttac tacttttgcc ttctgttctt tcaatagtct tcgtgctttc tgtggattac
      841 acggcatcag tggtttcccg tgtttactaa taacatagac tagcatatag cctacctcct
      901 tacggagtaa tctttccatc gccaatgtta ttcggcagtt tctcgttagc agcactgtcc
      961 ctacccatca ggactgttta accaccaacc gcagagcata agacttggga acgcatccta
     1021 tggtgcctat acatttgccg ataacgtagt gctcgaagca ctcaggctag tcaaatagag
     1081 cttttacaag ctcacggctt tagccgtggg gtatttgacc aaaagattca ctagctttgt
     1141 tctacatctc ttagtatcta gtacccagta tgctagagta gaaggaacat ttttcaatcc
     1201 gtactcttgc acaaacaact tgagctcgct gataggttct aatctaactc cccaatctct
     1261 gttgtagcgc gcatagtctc tcataaaagt atctatgatc actctctctt tctccgtaag
     1321 ctgaagatac ccagagatga attcttcgct ttttcttttc ggcatgacat ccaagaactt
     1381 cattgcagcg tctcttctac cttcaagaaa gggatgaaga tcgaaaagtt gtccaatgaa
     1441 cttcattagc tcgtatcgcc tcttagctat ctctttaagc gtatgcagat caattgaaca
     1501 agcttcaact attcttaata acacgccttt tcggcacaaa tagggaaagc tacccattct
     1561 atctcgaaga gcagagtaag ttgaaagaac tagcttcttc gcattatatg atgaaagctt
     1621 tccgagttca ttaatggcta tctcgaagct ttcttcgact ttggatttca catactcgtt
     1681 aacaatcggg aaaggatcat ttaagagatc tacggctatt tccagtccga tgattatctt
     1741 cctaagggtc acacctctta ttctccttgt tctgccgttc agcaagtaac tcaaattatc
     1801 ccacgccaca catgtttttc tgctcgtgtt cttaacattt tttgcgtgtt ttttcaggta
     1861 tgatctcgtc aagtcttcgt ttggatactc cgattgttca agctcaacaa ctgcaagcct
     1921 atcccgcagc gtttgcagtg gaccgtcctt ctcaaagcca gtcattcttt gtaattcgat
     1981 ggtcttgtat agccctttat ccttactctc atgttggata attgaaagag tgtctaatac
     2041 acacgaagca gagctaagct tttcatcgta gtaacccgaa taataggcag ctctctccgc
     2101 tagcttcaac gagtattcta tgttatcctg cttcatgtac catgaataat cgttgagtac
     2161 attaattaac ccctgtggat cttcaagttt ctttgccatt ttcaaggctt cttcaaacaa
     2221 atctcgagcc tgatctctgt tccccctctg agcagctctt cttgctcttc caactaggag
     2281 aagtattctc gcagagctct ctttctcgta tacttcactc cagaccctaa aaaccctgat
     2341 ttctctttct ggatggtaga gaccgtaata attggtaagc gcccatgcga cttctttgcg
     2401 aaccccagaa ggtattcttc caaactctct tctcagtttg ttgtatagtt cgtcattttc
     2461 accatttgac acaagcagct ttatagaaag caatagatag aagagactac gattcctcgt
     2521 gcttctattt agtccatgtt caatcatcga gacaatagca gaagaatctt ctccggcata
     2581 catgcaatca agaaccttga tgaactctct ccattctgag gtggggagtt tatccatgta
     2641 catattgaca ataggtcttg agtagaatat tccaaattcg ccattaagta gactaggtat
     2701 cttgcgattc aactcgactc tccttcagct actgcaaaga aagaaatagg tttgttcttg
     2761 agttttcgcc aatgtaacaa gaacaagctc taacacatga agaactatag tgttatgcat
     2821 caaaaaatac aactgtcttt gagtgcaaag aaacatagac ctataattgg ctaattaaga
     2881 gcattatatc agataggaac ttgaaaatca tatccaaaga tctataatct cgactgtact
     2941 gaaaaaattg gctgtccgga ggtcttggga aaatgaatgg gtccatcatg ttctgaagga
     3001 ggttatgaag tgtggaagat ctgaaggaag aaatcaaaga gttttacgag ttcttcgact
     3061 attcttggat gaaaaacatt gaagagccct ggtgtcacac agcaatagtt atctggtaat
     3121 caattacctg agtacaacaa ttcccgaact ttcaaa
//
LOCUS       DAOQYI010000109         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_33298_length_3110_cov_61.580687, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000109 DAOQYI010000000
VERSION     DAOQYI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33298_length_3110_cov_61.580687"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            597..1439
                     /locus_tag="PKL93_11130"
     CDS             597..1439
                     /locus_tag="PKL93_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ71852.1"
                     /translation="MGTVLTIANNKGGVGKTLVTLAVASTITWLQKNDPTKLPQHMRK
                     APILVVDTDPQANTTEALGIDPVEVAVNILELYENPSVIETTSGLSLLQASPKHKNIV
                     VIPSSIKLESISNTLPSQIDGRTRLKDAIDRVRDNYSLIIIDTPPSLGIFTQNAVVAA
                     DNILIPLTLTKHALLGVSNMLTFLVTAKKSQNPRLNVLGLLVNAYDKRYRLQTSLLKA
                     IQKEYDSFSFDTIIPTSSRMVENIAARRNLLHNIPADHRGILIDLLNELFHRVDTPVE
                     FHKG"
     gene            1445..>1691
                     /locus_tag="PKL93_11135"
     CDS             1445..>1691
                     /locus_tag="PKL93_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71853.1"
                     /translation="MANKEKKKRDTTLDDILESTENVNQIGVEQARSLSRAETYEKNV
                     ESIWGVPAPVATKRDATQTPLKDSRDIGGEDHPSLTKN"
     assembly_gap    1692..1791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1792..3110
                     /locus_tag="PKL93_11140"
     CDS             <1792..3110
                     /locus_tag="PKL93_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71854.1"
                     /translation="GEDHPSLTKNKGEDIVRSKEDSSIELPSIEESSIEVSSSKEQSS
                     IESPSIEENSIEESGMEEHTSIELSSIEEQSSIEQKAIDDPSQLTTDSHIILYTIRNF
                     PTFIRQRTDLNPNEKLVLEHIFDRALLEGSHIITMTNSDIETNLSISYKWAGKTLQSL
                     VQKNWIDHKLSLSKTNKSKVSVEKALANFVEEKSLNIDLPTLLNQMFNVESMEESSID
                     PYMFVLLVSKNIYLRTNKQNIVQSMEQNSIDNVDTKNLLLYFSSYQSLKQILTYAWLK
                     GFDIQQISRKLLFGISDMFSDNTYGDKIPLRSQKVEEIVAQGIFYAAPKAKKNKWGYL
                     EKTLKDGWAMDMTIDEHDRSIEELEMANHMCESPEKVSLFGLDEIRDLLSAFPALTKT
                     KDVNAKNIEKIRKDIKDFFIFSPKAVEDFLEKNGFKSRKPSDDLRA"
BASE COUNT         1010 a          591 c          584 g          825 t
ORIGIN      
        1 agagctaatt ctcaagacga gtggatacga agttattagg atcggcgcca aagacctgga
       61 tgatgacgag atgatggcat tgtatcttca aaatatctca aactctatcc aataaagagt
      121 ttgctaatct atagaattta gttctataga tgtttattct atagaataag attccataga
      181 attatcgtct ctagactatt attctataga acaatattct atagattatt gatacccctt
      241 tatgaatttc gaatagcgac tcagaaacct agacaagttt ggttattatg atcacaaatc
      301 acacagtatc caaaagtgaa tttgtcgccc tcaattcaag ctactcaaca ctataactat
      361 acaaatctat tctaattgag aacctcatca tatcaattga agggcacgct ttgtgataat
      421 ttcgcccgaa caaccattga atacctgctt aaagttacgt tcgaaatata tatagaagta
      481 cgcatatttc cttatttaca gccctctttg cgcttgattg cttgcattta tattttacaa
      541 atggtttgtg atataattct gaggaatgct ggggatctac tgggggctgt ttttaaatgg
      601 gtactgttct cacaattgca aacaataaag gcggggtcgg gaaaacactg gtaacgcttg
      661 ctgtagccag cacaattacc tggttgcaaa agaatgaccc gactaagctt ccacaacata
      721 tgcgtaaggc gcctatcctt gttgtagaca ctgatcctca agcaaatacc accgaagctc
      781 tgggaatcga tccagtggaa gttgctgtca atattcttga gttatacgaa aatccgtcgg
      841 taattgagac aacgagtggt ctatcacttc ttcaggcctc tccgaagcat aagaatatcg
      901 tcgtaattcc ctcttccata aagcttgaga gtatcagtaa tactctaccc tcgcagatag
      961 atggaagaac tcgcttaaaa gacgctattg atcgtgtgag ggacaactat tcactaataa
     1021 ttattgatac gcctccttca ttagggattt ttacccagaa tgcagttgta gctgcagata
     1081 acattcttat tcccttgacg ctaacaaaac acgcactcct cggcgtaagt aatatgctca
     1141 catttcttgt aactgcgaag aagagtcaga atccacgatt gaatgtatta ggattgttgg
     1201 ttaatgcgta tgataagcga tatcgtcttc agacatcatt actaaaggcc attcagaaag
     1261 aatacgattc cttctccttt gacacgatta tcccgacatc ttccaggatg gttgaaaaca
     1321 tagctgctcg gagaaaccta cttcacaaca tcccagctga tcatcgcggt attctgattg
     1381 atttactgaa cgaactcttc cacagagttg acaccccagt agaatttcac aagggatgac
     1441 gcttatggca aacaaagaaa agaaaaaacg agatactacc ctggatgaca ttctcgaatc
     1501 aaccgaaaac gtgaatcaaa taggcgttga acaggcgcgc tcactctcgc gcgctgaaac
     1561 ttatgagaag aatgttgaat caatttgggg tgtccctgca cctgttgcaa cgaagcgaga
     1621 tgccacacaa actcctttga aagattcccg agatattgga ggggaagatc acccatcatt
     1681 aacaaaaaac annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaggggaag
     1801 atcacccatc attaacaaaa aacaaaggag aagacatcgt tcgttctaag gaagatagtt
     1861 ctatagaatt accatctata gaagaatctt ctatagaagt atcttctagt aaggaacaaa
     1921 gttctataga atcaccgtct atagaagaaa attctataga agaatccggt atggaggaac
     1981 atacttccat agaactaagt tccatagaag aacaaagttc tatagaacaa aaagccatag
     2041 atgatccttc ccagctaacg actgattcac atattatcct atatacgatc aggaatttcc
     2101 caacctttat aaggcaacga accgatctga atcccaacga aaaactggta ctggaacata
     2161 tctttgatcg cgctcttctt gaaggatccc acattataac aatgacaaac agcgatattg
     2221 aaacaaatct ttccatctcc tataaatggg caggcaaaac actgcagtct ctagtgcaaa
     2281 agaactggat tgaccataag ctatcgttga gtaaaacgaa taagagcaaa gtctctgttg
     2341 agaaagcact tgcgaatttc gtagaggaga aatcgttaaa cattgatctt ccgacattac
     2401 ttaatcaaat gtttaacgtc gagtctatgg aagaaagttc catagatccc tatatgtttg
     2461 tattgttagt tagtaaaaat atatatctaa gaactaacaa acaaaacata gtacaatcta
     2521 tggaacaaaa ttccatagat aatgttgaca ctaagaattt gttgttgtat ttttcttcgt
     2581 accagtcctt gaagcaaata ttgacttatg cctggctcaa aggattcgat atccagcaaa
     2641 tatcaaggaa gcttcttttc gggattagcg atatgttctc agacaatacc tatggggaca
     2701 aaattccttt gaggtcccaa aaagtcgaag aaatagtagc ccaggggata ttttatgctg
     2761 ccccaaaggc aaagaagaat aagtggggat atctcgaaaa gactttgaag gacggctggg
     2821 cgatggatat gactattgat gagcacgata ggagtattga agagctagaa atggctaatc
     2881 atatgtgtga aagtccggaa aaagtgtctc tctttggcct ggatgaaatt cgggatttac
     2941 tgagtgcctt tccggctcta acaaagacca aggacgtaaa tgcaaagaac atagaaaaaa
     3001 ttcggaagga tatcaaagat ttctttattt tttcgcccaa agcagtggaa gacttcctgg
     3061 agaagaacgg ctttaagtca agaaagcctt ctgatgactt gagggcataa
//
LOCUS       DAOQYI010000110         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_34237_length_3052_cov_87.558892, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000110 DAOQYI010000000
VERSION     DAOQYI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34237_length_3052_cov_87.558892"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKL93_11145"
     CDS             complement(<1..416)
                     /locus_tag="PKL93_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ71855.1"
                     /translation="MELRGKLLDVSNLKVSFYTYRGVVKALNGVELWMDSGERLGVVG
                     ETGCGKSVTSLAVMRLIEQPGEIQEGKIIFNGRNLVELSEEKMNNIRGVEMSMIFQEP
                     RSSLNPVMKVGYQIGEAIAKSKKIRIKNTYPEVKEML"
     gene            complement(421..1293)
                     /locus_tag="PKL93_11150"
     CDS             complement(421..1293)
                     /locus_tag="PKL93_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71856.1"
                     /translation="MEKTVKVSKPFMDDLKHTAFLWRKSRLTMIGSAIILVFLLMAAF
                     APLIAPYDPVHQDLQIKLQAPSWQHLFGTDQFGRDIFSRVIMGSRIALWIIFLVSVIS
                     GSIGIIVGVTAGYFGGIVDEVLMRITDMFLAFPSLVLAMAFAAMLGPNLTNTIIAISI
                     VTWTTYARLSRAEAIKVKSQPYIEAIRAVGGGNLRIMFLHVLPMCISPVLVQLTLRMG
                     TIILTAASLGFLGLGVQPPTPEWGAMVSDGRNYLIDQWWISTFPGIFIAFVVLGFNLL
                     GDGIRDMLDPRLRR"
     gene            complement(1299..2309)
                     /locus_tag="PKL93_11155"
     CDS             complement(1299..2309)
                     /locus_tag="PKL93_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71857.1"
                     /translation="MNFGQFLLRRLFLMIIVLFGVACIVFFIANVIPADPVGAILGGN
                     APPEAVDRLKAKLGYDQPLIIRFGNFITGAVRGDFGISLKTSNPVMEDIRNYFPATME
                     LALVAITISIILGITLGILSAVHRNKAIDQFSRIFSILGVSMPVFWIGLLLLLVFYFK
                     LGWLPGSGRLGFFTYPPPRVTGMYLIDSIIAGQWDTFKEALMHILLPAFVLGYNATAS
                     IARITRASMLDVLRQDFIRTAKSKGLKKNVVIYRHALRNSLIPTVTIIGLVFGSLLEG
                     AVLTETVFSWPGLGRYITTGMLFLDYPAVMGGTLYIALIYSIANLIVDILYALLDPRM
                     RM"
     gene            complement(2387..>3052)
                     /locus_tag="PKL93_11160"
     CDS             complement(2387..>3052)
                     /locus_tag="PKL93_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ71858.1"
                     /translation="REAVRYSINYEEIVEDIMLNNALLVQGFINKGYFGYVEENPFYQ
                     DIEKAKALLAEAGYPDGFEVELLTSNTDTRKAEAEKIQADLALAGIKANIVIMQSSQM
                     YAKYRAQGHQMIIAGWGNDYPDPDNLAMAHASYRANQLAWRNAWFDDYAATLCEWGQV
                     EPNPDKREQIYKDLTEYWFHNGPFAMLYQTVEFWGIRKEVKNFEEAAFGYGMLFDFTK
                     VSK"
BASE COUNT          789 a          764 c          691 g          808 t
ORIGIN      
        1 agcatttcct taacctccgg gtaggtattc ttgatcctga tcttctttga cttggcgatt
       61 gcctccccta tctggtaacc tactttcatt acgggattta gggaggatct tggttcctgg
      121 aagatcatcg acatttctac gcctcgtatg ttgttcattt tctcttcaga caactctaca
      181 aggttcctac cgttgaagat tatctttccc tcctggatct ctccgggttg ttcaatcaag
      241 cgcattaccg ccaacgaagt gaccgacttt ccacagcccg tctcccccac gactccaagt
      301 ctttcgccag agtccatcca gagctcgaca ccgttgagcg ctttcactac tcctctgtat
      361 gtgtagaaag atactttgag gttagataca tctaatagct ttcctctaag ctccatataa
      421 ttacctcctc aaccttggat cgagcatgtc tcttattccg tcaccgagga ggttgaatcc
      481 aagaacgaca aaggcgatga atatccccgg aaaggtagat atccaccact ggtcgatcag
      541 gtagtttctg ccgtcggaaa ccatggctcc ccattcgggt gttggaggct ggacacctaa
      601 cccgaggaag ccaagacttg ctgcggtgag gattattgtg cccattctga gagtcaactg
      661 aacaagaacg ggagaaatac acattggcaa aacgtgaagg aacattattc taagattgcc
      721 accgccgacc gcacgtatag cttcaatata tggctgcgat ttgactttta ttgcctcggc
      781 tcgggagagc ctggcgtagg ttgtccacgt gacaatggat atggcaataa tcgtgtttgt
      841 cagattcgga cccagcattg cagcaaaggc cattgctagg accagactgg gaaaggccag
      901 aaacatgtcc gttatcctca tgagcacttc gtcgacaatg ccaccgaaat aacctgccgt
      961 aacaccaaca ataattccta tggacccgct gatgaccgat accaggaaga ttatccagag
     1021 cgcgatcctg gatcccatga taaccctgct gaatatgtcc ctcccgaact ggtccgttcc
     1081 gaagagatgc tgccagctcg gagcctgcaa ttttatctgg agatcctgat gaaccgggtc
     1141 gtaaggagct ataagtggcg caaaggctgc cataagaagg aaaacaagga ttatagccga
     1201 accgatcatc gtgaggcggc tctttctcca gagaaaggct gtatgcttca aatcatccat
     1261 gaatggctta gatactttta cggttttttc catctagatc acatcctcat ccttggatcg
     1321 agaagagcgt aaagaatgtc cacgataaga ttcgcaattg aatatatcaa ggctatatac
     1381 aatgtaccgc ccattactgc cggataatca aggaagagca ttccggtggt tatatatcta
     1441 cccagacctg gccaggagaa gaccgtttcc gtaagaacgg ctccttccag cagacttcca
     1501 aatacgagtc cgattatcgt aacggtcggt atcaaagaat tacgcagtgc atgtcggtaa
     1561 ataactacgt tttttttcaa gccctttgat tttgccgttc ttataaaatc ctgcctcaac
     1621 acgtcgagca tactggctct ggtaattcta gctatggagg ccgtcgcatt gtaaccgagg
     1681 acgaacgctg gaagaagaat atgcataagg gcttctttga atgtgtccca ctgccctgca
     1741 atgattgagt ctataagata catgcccgtc actcttggag gcggataggt gaagaagcca
     1801 agtcttccgc tcccggggag ccagcccagc ttgaaataaa acacaagcaa cagaagaagc
     1861 ccaatccaga agaccggcat cgagacccct agtatagaga agattctaga aaactggtct
     1921 atggctttgt tacggtgaac ggccgacaag atccccagag ttatccccag tatgatcgag
     1981 atcgttattg cgacaagagc cagttccatc gtggcaggga aatagttcct gatatcttcc
     2041 ataactggat tggacgtctt caaagaaatg ccgaaatctc ctctgactgc tccggtgata
     2101 aaattgccga accttattat tagaggctga tcgtagccca gctttgcctt caatctatct
     2161 accgcttccg gcggagcatt tcctccaaga atggcgccga ccggatcggc aggaatgaca
     2221 tttgctatga agaagacaat acaggcgact ccaaagagaa ctatgatcat taagaatagt
     2281 cttctgagta agaattgtcc gaagttcata gaattacccc cgcatcaata tctgattttt
     2341 ttgggaaaga atacgccccg ctcaaaagag cggggcgggg gatactttac ttagatacct
     2401 tggtgaagtc gaacagcatt ccgtaaccga aagcggcttc ttcaaagttc ttgacttctt
     2461 tgcgaattcc ccagaactct actgtctggt aaagcattgc aaagggtcca ttgtggaacc
     2521 agtattctgt aagatccttg tagatctgtt cacgcttgtc tggattgggc tcgacctgac
     2581 cccattcaca aagcgtcgcc gcgtaatcgt cgaaccatgc atttctccat gcgagctggt
     2641 ttgccctgta gcttgcgtga gccatcgcaa ggttgtccgg gtctgggtag tcgttacccc
     2701 agccggcaat tatcatctga tgaccctgtg ccctgtactt tgcatacatc tgagaagact
     2761 gcattatcac gatatttgcc tttatacctg caagagccag gtcggcctgg atcttttcgg
     2821 cttcggcctt tcttgtatcc gtgttgctgg tcagcagttc aacttcgaat ccgtcaggat
     2881 aaccggcctc tgctaggaga gccttggcct tctctatatc ctgatagaat ggattctctt
     2941 caacatatcc gaagtagccc ttgtttatga aaccctgcac gaggagagcg ttgtttagca
     3001 taatatcttc aactatctct tcgtagttga ttgagtacct tacggcttct ct
//
LOCUS       DAOQYI010000111         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_35017_length_3004_cov_75.624619, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000111 DAOQYI010000000
VERSION     DAOQYI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35017_length_3004_cov_75.624619"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PKL93_11165"
     CDS             complement(<1..1106)
                     /locus_tag="PKL93_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNQ71859.1"
                     /translation="MRQGVLSIDLGTMGVKLSLVSLEGEIQKSAYTEYPIISESPGQA
                     EQDSALWWEGIIDCVKKLTDSDNNLPHLIKAISICGQMHTHVYLDSEDRPIGPSITWL
                     DQRSSEIIEEWQSDGRAARLFDLTWNFPTTTYAAPQICWVKKHRPGVFSRTKSILIAK
                     DYIKFLLTGNKVTDPSDASGTAVFDIRTNQWSREALELIDLDGDLLPEVIPSARIIGH
                     VTEKAARETGLIQGTPVVNGGSDHSVAELGSGLLGEGEVSCIVGTAGVVAACTSKPVI
                     DPKKRIMCWSYPLEGYWDILGITQTAASSLTWFRNTFDKERNSEVFDEYSSLAKGVSL
                     GSEGLVFLPYLMGERTPLWDSKARGVFFGLTMKHS"
     gene            1602..2606
                     /locus_tag="PKL93_11170"
     CDS             1602..2606
                     /locus_tag="PKL93_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ71860.1"
                     /translation="MNKKILMLLFCVLFTVAVLAANYEIAVVVKIGGIPWFNRMEVGV
                     KDAADELGVNAYQIGPSDADPAQQVKIVEDLIAKGVDAICVVPNDAKALEPVFEKARA
                     NGIIIITHESPEQKGGDWDVETIDNVKFGEANFEKLAQLMGGEGEFAVFVGGLTVPLH
                     NFWADVGLAYVAEKYPNMKLVTERIPCGESVELSYQKTLELLNAYPNLKGIVGFGSLG
                     PIGASQALNARNMKGKVQIVGTVIPSHADPYLKQGLMQWGYLWDPRDAGFAQVYLAKT
                     LLDAKAALAEAGIPEGIVPAVVDGFEIPDLGKATLEGNIVKFDAQVDITAENALSYGF
                     "
     gene            2650..>3004
                     /locus_tag="PKL93_11175"
     CDS             2650..>3004
                     /locus_tag="PKL93_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ71861.1"
                     /translation="MAEKLLEMRNISKRFGGVLALDSVDFEIEKGEIHCLVGENGSGK
                     STLIKIISGIHSPDPGGEIYVDGRRISHQKSSNSVREGIQVIYQDLSLFPNLTVAENI
                     AISSRVETGSRLMKWK"
BASE COUNT          797 a          682 c          746 g          779 t
ORIGIN      
        1 gagtgtttca tggtcagtcc aaaaaagacg ccccttgctt tggaatccca cagaggggtt
       61 ctttcgccca tgagataagg tagaaaaacc aggccttccg accccagaga aacacctttt
      121 gccagggagg agtattcgtc aaagacttcg ctgtttctct ctttgtcgaa ggtatttcta
      181 aaccaggtta agctggaagc ggccgtctga gttatcccca gaatatccca gtaaccttca
      241 agtggatagc tccaacacat gatccttttc tttgggtcta ttaccggctt ggatgtgcaa
      301 gcggcaacga caccggccgt gccgactatg catgaaacct ctccttcacc gagaaggccc
      361 gaacccaatt cagcaactga gtggtccgaa cctccgttca ccaccggtgt cccttgaatc
      421 aggcctgtct ctcttgccgc tttctcggtg acatgaccga ttattctcgc tgaaggaata
      481 acctccggaa gaagatctcc atccagatca attagttcaa gagcctccct gctccattga
      541 ttcgttctta tgtcgaatac agccgttccc gaggcatccg agggatctgt aaccttgttc
      601 cctgtgagca gaaactttat gtaatccttt gcaatcaata tcgacttcgt acgggaaaaa
      661 acacctggcc tatgcttttt cacccagcat atttgtggag cggcgtaagt tgttgtgggg
      721 aagttccagg tcagatcgaa gagccttgcg gccctgccat cgctttgcca ttcttctatt
      781 atttcgctgc ttctctgatc tagccaggtg attgaagggc ctattggcct gtcttcggag
      841 tctagataga catgggtatg catctggccg caaatagaaa tcgctttgat tagatgaggg
      901 agattgttgt cactatcggt caacttcttt acacaatcta tgatcccctc ccaccatagc
      961 gccgaatctt gttctgcctg tcccgggctt tctgagatga tggggtactc cgtgtatgcc
     1021 gatttctgaa tctcaccttc aagagagacc aatgaaagtt ttactcccat ggttcccaga
     1081 tcgattgaaa gaactccttg cctcatcgtc tcctccacag gtacattcca aaatcactat
     1141 tgagtaaaac ggatcactaa agtgataact tcatatcata gtctattacc tagagcttac
     1201 cagataatct gcatttgaag aaatccgatt aggaacaaat atcccatagt tcaaacgatt
     1261 aggagtgatt tccgacgaat aatactatgt aatgttagct tagacagtgt caataaaaac
     1321 agatctttga ctggtaatac agctgcggga aggcgataga tcaagttgat catccacaat
     1381 tgtcccggaa caatggatga gataatccgg aaaacgagag tatttatcgc tacagggtct
     1441 tcatcagccg taagttcgaa taaccagtaa tcttcggata tggatctgtc agagtgaatc
     1501 gatataattg aaatgttgat cacaagtaat catgtgaact acgatgcttt gtcttatgat
     1561 ctttgggccg gcctaccggt cacatagaag gagggatgcg aatgaacaag aagattctga
     1621 tgctcctgtt ctgtgtcttg tttacagttg ccgtcttagc agcgaactat gagattgccg
     1681 tcgttgtcaa gatcggcgga atcccctggt tcaacagaat ggaagtcggt gtaaaagatg
     1741 cagcagatga actcggcgtg aatgcctacc agatagggcc ttccgatgcg gatcctgctc
     1801 aacaggtgaa gattgttgaa gaccttattg ccaagggcgt cgatgccatc tgtgtagtac
     1861 ctaatgatgc gaaagctctt gaacctgtat tcgaaaaggc aagagcgaac ggtatcatta
     1921 tcatcacaca cgagtcacca gagcagaagg gtggcgactg ggacgtagaa acgatagaca
     1981 acgtcaagtt tggagaagct aacttcgaga aacttgctca actaatgggc ggagaaggtg
     2041 agtttgccgt tttcgtcgga ggactcacag ttcctctcca caacttctgg gcggacgttg
     2101 ggcttgccta tgttgcggag aagtatccaa atatgaaact ggtaaccgag agaatcccat
     2161 gtggtgagag tgttgagctt tcctatcaaa agacactcga gctgctaaat gcgtatccta
     2221 atttgaaagg tatagtcgga tttggttcac tgggaccgat cggagcgtcc caggcgctca
     2281 acgcgagaaa catgaaggga aaggtacaga tagtcggaac ggttatccca agccatgctg
     2341 acccgtatct caagcaagga ctgatgcagt ggggttacct gtgggatcca agagacgctg
     2401 gattcgcgca ggtctatttg gcaaagacat tgctcgatgc caaggccgct cttgctgagg
     2461 cgggaatccc cgaaggcata gtgccggctg ttgtcgatgg attcgagatt cccgatcttg
     2521 ggaaggccac gctggaagga aacattgtga aattcgacgc gcaagtcgat ataactgctg
     2581 aaaacgccct ttcttacggg ttctgatttc gagagggggt acgccccctc tctttctggt
     2641 gatctgatta tggccgaaaa actgttggag atgagaaata tttcgaagag gttcggggga
     2701 gtattggccc tcgattcagt cgattttgaa atagaaaagg gggaaatcca ctgtctcgtt
     2761 ggcgaaaacg ggtctggaaa gagtacccta atcaagataa tctcgggtat tcactcaccc
     2821 gatcccggtg gtgagatcta tgtcgacgga agaaggataa gccaccagaa gtcttctaac
     2881 agtgtgaggg agggaatcca ggttatctat caggatcttt cgctctttcc aaatctgacc
     2941 gttgcagaga acatagctat ttcttcgagg gtggaaaccg gaagtcgtct gatgaaatgg
     3001 aaag
//
LOCUS       DAOQYI010000112         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_36136_length_2937_cov_77.738376, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000112 DAOQYI010000000
VERSION     DAOQYI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36136_length_2937_cov_77.738376"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..1305
                     /locus_tag="PKL93_11180"
     CDS             448..1305
                     /locus_tag="PKL93_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNQ71862.1"
                     /translation="MKIDLRCIPCNLNSLLRILDQNHAGEEEKKQVVRTFMKEVAEMD
                     WSDTPIDIGRKVGEVLEPLFGDSDAFRDIKKMSNDNLLEIYDDLKTELLKLDDPLIGA
                     LKLSVAGNIIDVAPGHKIDIHSTMKDALSRPFAIDDLQELADRIRKANSLLIVGDNCG
                     EAVLDKLLLDIAKVPNSYFSVRTKPALNDITMKEALEIRMDEVATLMDSGADAPGAQE
                     KTMKRAFSEVYFSADVVISKGQGNLEGLSSVKREIFFLLMAKCEVIGGFLGVPKGSFV
                     AYKKAESRG"
     gene            1307..>2937
                     /locus_tag="PKL93_11185"
     CDS             1307..>2937
                     /locus_tag="PKL93_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ71863.1"
                     /translation="MELFKDFPVLVVDDDLRSENTGGRATREIIKELQKRGFSVIESY
                     SGYDCRIEFMSHSNVSCVLLDWDLVIKPDAEFLGPGEIIEIIRGRNMLIPIFLMTEKL
                     RVKEIPLEIVSQIDGYVWKLEDSPSFIAGRIEEATERYMDELLPPFLKELIRYVDEFK
                     YSWHTPGHSGGEAFLKSSTGKIFHKFFGENIFRSDLSVSVPELGSLLEHTEAIGESEK
                     SAAKIFGSDETYFVTNGTSTSNKIVFHYCVTPGDIVLIDRNCHKSIMHSIIMTGAIPI
                     YLTPSRNSLGIIGPIHEENFEWSEIEKAIKESPLVEDKENYRIKLAVITNSTYDGLCY
                     NARTILDRLEKVVDFVLFDEAWYAYAKFHPMYLGRFGMSSDIDRERSPVVFSTHSTHK
                     LLAAFSQGSMIHVKDGRKRVDHGRFNEAYMMHMSTSPQYAIIASLDVAAKMMAGNAGR
                     FLIDETIQEAIIFRKKMKHLKKEIESKETDRKRRWWLEIWQPDKVSIETESGERKTFD
                     LEDIDESILKDRPDCWYLKANEDWHGFGKLDNDYAL"
BASE COUNT          842 a          646 c          717 g          732 t
ORIGIN      
        1 cgaattctct gactttcatt tcttttgagc atcctgtcac aaaagtgtta gcatcgacgg
       61 aaaacgttgt aatcagtaat gatcagcgga gactggttct ttacccgaag cagtaggaac
      121 tggcatgcga agcagactgg ctacgtagat cttccccgcg aagcgggcat tgcgacctgg
      181 cgtgaccttt gccagccttg cgtcctgccg caggcggcat tgcgaccgcc gatgattttc
      241 ggcgccttgc gtctagtgtt ttatggtagc atcacttccg gcgatgttct tcgcgcatca
      301 cttaagctct tgccagtctg ctgatttcgg atgacggaga accgttgacg gttgacctga
      361 taggcctttt gcagatgtag tttgcatgtc aaatgaacac aggttgcgat tactaaaaca
      421 ctgtataatt cattgtgagg tgattgaatg aagatagatc tcagatgtat tccctgcaat
      481 ctaaacagcc ttctcaggat tcttgaccag aaccatgccg gagaggaaga gaaaaagcaa
      541 gtagtaagaa ccttcatgaa agaagtcgcg gaaatggact ggtcagatac tccaatagac
      601 atcggccgaa aggtcggtga agtacttgaa ccactctttg gtgatagcga tgcctttaga
      661 gatataaaga agatgtctaa tgacaatctt ctggagatct acgatgattt aaaaactgag
      721 ctcctcaaac ttgacgatcc gttgatcggt gcgctgaagc tttctgtcgc aggtaatatc
      781 atagacgttg ctcccggtca caagatcgat atccactcca cgatgaagga tgctctttcc
      841 agaccttttg cgatagacga tcttcaggaa cttgccgata gaatccgcaa ggcgaactct
      901 ttgctgatcg tcggagataa ctgtggcgaa gccgttttag acaagctcct tctggatatt
      961 gcgaaagtgc ccaatagcta cttttctgtc agaacaaaac ctgcgctgaa cgatattact
     1021 atgaaagagg cgcttgaaat aagaatggat gaggttgcga cactaatgga ttccggggcg
     1081 gacgcgcctg gcgctcagga aaagacaatg aagagagcgt tttccgaggt ctattttagc
     1141 gccgatgtag taatctctaa aggacagggc aaccttgagg gtttgagttc cgtgaaacgt
     1201 gagattttct tcttgcttat ggcaaaatgc gaggtaattg gaggcttcct cggagttcca
     1261 aagggttcgt tcgttgccta caaaaaggca gaatcgaggg gatagaatgg agcttttcaa
     1321 agattttccg gtcctagttg tcgatgacga tcttcgttca gaaaatactg gtggaagagc
     1381 tacaagagag ataatcaaag agcttcagaa acgaggtttt tccgtaattg aatcctattc
     1441 aggctacgat tgcagaatag agttcatgtc acactcaaat gtaagctgtg tcttgcttga
     1501 ttgggaccta gtcataaaac ccgatgcgga atttctaggc cccggagaga taatagagat
     1561 aatccggggt agaaatatgc ttattcccat ctttcttatg accgaaaaac ttcgagtcaa
     1621 ggaaattccg ctcgaaattg taagccaaat cgacggctat gtctggaaac tcgaagacag
     1681 cccctctttc attgcaggca gaattgaaga agcaactgaa cgctacatgg acgagcttct
     1741 tccgccattc ttgaaggaat taattagata cgtagatgaa ttcaagtatt cttggcacac
     1801 tcccggccac agtggtggag aggcttttct gaagtcttct accgggaaga tctttcataa
     1861 gttcttcgga gaaaacatat tcagaagtga cttatccgta tcagttcccg agcttggatc
     1921 gctccttgaa cataccgagg cgatagggga gtccgagaaa tccgccgcaa aaatctttgg
     1981 ttccgacgag acctacttcg taacgaacgg cacatcgaca tcaaacaaga tagttttcca
     2041 ttactgcgtt acacccggag atatcgttct cattgacaga aactgtcaca agtcgattat
     2101 gcattcaatt atcatgaccg gtgcgattcc catctatctc acgccgagca gaaactcact
     2161 gggaataatc ggaccgatac atgaggagaa tttcgagtgg agcgaaatag aaaaggcaat
     2221 caaggaaagc ccccttgtag aagacaaaga gaattacaga ataaaactgg cagtcattac
     2281 aaattccacg tacgatggtc tctgctacaa cgcaagaacg atcctcgaca ggcttgagaa
     2341 agtggtcgat ttcgttcttt ttgacgaggc ctggtacgcc tacgcaaaat tccaccccat
     2401 gtatcttggc cgattcggaa tgtcttccga tatcgacagg gagagatccc cggtagtctt
     2461 ttcaacacac tccacccaca agctcctggc agccttctct caaggctcca tgattcacgt
     2521 gaaggacggg agaaagaggg ttgatcacgg gagatttaac gaagcctata tgatgcatat
     2581 gtcgacttcc ccccagtacg cgattattgc atcgctcgac gtcgctgcaa aaatgatggc
     2641 cgggaatgct ggaagattcc ttatagatga aacgattcaa gaggcaatta ttttcagaaa
     2701 gaagatgaag cacctcaaga aagagataga atccaaagag accgatcgaa agagacgctg
     2761 gtggttggaa atatggcagc cggacaaagt ctctattgaa accgaaagcg gcgagcgaaa
     2821 gacctttgat ctcgaggaca tagatgagtc catcttgaag gacaggcccg actgctggta
     2881 tctcaaggct aacgaagact ggcacggatt tggaaagctc gacaatgact atgccct
//
LOCUS       DAOQYI010000113         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_36317_length_2929_cov_47.632220, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000113 DAOQYI010000000
VERSION     DAOQYI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36317_length_2929_cov_47.632220"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..1742)
                     /gene="miaB"
                     /locus_tag="PKL93_11190"
     CDS             complement(435..1742)
                     /gene="miaB"
                     /locus_tag="PKL93_11190"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNQ71864.1"
                     /translation="MKVAFRTFGCQMNISDTEAMMGLLRLAGHSIVNSEEEADAVIVN
                     TCAVREKSEDKLYGKLGQLKAQKKRNAKLIVGVCGCVAEKNSDELLTHREVSFVFGTR
                     AISRVAEMLSRASAGERFIELGDFVDELDSETPRLRTSNHHAWITIIYGCDKFCSYCI
                     VPYTRGREKSRRMEDILAEAHELAKLGYREITYLGQNVDSYGKDLGDGSSLATLLKET
                     LKIEEIERLWFLTSYPKDFSDELIEVIASSERISRSIHLPVQSGSDRILKAMNRGYTR
                     EYFLNLTRRVKERVQDSSLSTDIIVGFPGETDDDYNDTVSLIKEVRFERVNLAMYSPR
                     EGTLSARKLADDVPQQVKTKRLNDLLALQKHINREENEKYIDEVLDVIGEGKIKGNGK
                     IYGRTMNNKIVIYEADLDKIGVPVKIRIESVSAGPLYGEIVGR"
     gene            1904..2680
                     /locus_tag="PKL93_11195"
     CDS             1904..2680
                     /locus_tag="PKL93_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNQ71865.1"
                     /translation="MFARAYETASSFTRPVVVLNRRGDGKVSATCGTFVILNSRGWII
                     TVAHLWQSYFLFKKQQANPNETPEAQRITNHSFWWGKDGITLKDIRPFPEYDLVVGRL
                     EPFETSEVKEYPEFIKAEDMRIGTSLCRVGYPFSRIKATFDDKQSRFLMDSKSLPSLY
                     PIEGIYTRSVSAPAFKGAKKIAKFIETSSPGLLGQSGGPILDVTGRVWAVQSRTVHLP
                     LGFSPKARKEGREIEENQFLNAGWGVHIENINEVVQSGIG"
BASE COUNT          782 a          684 c          645 g          818 t
ORIGIN      
        1 cgagaccgag tgctttcata tatgaacctc ccatatacag ggtttcgtcg aaattgcttt
       61 ctattaagtc agatggcgta gtaagagatc tccgaagaca ttatcgacat gaaagtgttt
      121 tacagttctg agaaccttac gtaccttcat gctgataaga gccgtccttg gtccttcgcc
      181 caggatcatg aacccgtact tcgaagagca gttgcaagtt gcctgttcca ggttgcaagc
      241 cctaaatcaa agaaacgtca aatgccgtta tgctgaaacc agagtcgccc tttgagaaaa
      301 acggttcttc gttccgacgc ttcgcgtcca ggttcttggc aaagatcaga ggttagaggg
      361 ggagaggtca gaggtcggaa gagcgaaaag atcttggtct tcacaactct atcctcatac
      421 ctcttatttc tgagtcaccg tccaactatc tctccgtaca acggtcctgc agatacactt
      481 tctattctga ttttaacagg aacccctatt ttatccaggt cagcctcata gataactatc
      541 ttgttgttca tagtccttcc gtagatcttg ccgtttccct ttatctttcc ctcgccgata
      601 acatcgagca cttcgtctat gtacttctca ttctcttctc tatttatgtg tttctgaagg
      661 gccagaagat cgttaagtct cttggtcttt acttgctggg gaacatcgtc tgcgagcttt
      721 ctggccgaga gcgttccctc tctcggagaa tacatggcaa gattgactct ttcgaaccgg
      781 acctctttta ttagcgaaac cgtatcatta taatcatcat ctgtctctcc gggaaatcca
      841 acgattatat ctgtgctgag tgaagaatct tgaactctct ccttgacccg gcgagtaagg
      901 ttcagaaaat actctcttgt atagccccta ttcatggcct tgagtatcct gtcgcttcca
      961 gattgaacag gcaggtgtat tgaacgcgaa atcctttctg aagaggcaat cacctcgata
     1021 agctcatcac tgaaatcttt gggataagat gtcagaaacc acaggcgctc gatctcttct
     1081 attttcaaag tctccttcag gagagttgca agcgatgacc cgtctccgag atctttaccg
     1141 tacgaatcga cattctggcc tagataggtg atctccctgt atccgagttt ggcaagttca
     1201 tgtgcttcgg cgagaatatc ttccatcctg cgactcttct ctctgcccct agtataggga
     1261 actatacagt aagaacagaa cttgtcgcaa ccgtatatga tggttatcca tgcgtgatga
     1321 ttgctggtcc ttaaccttgg agtctcagaa tcgagctcat ccacgaaatc tccaagctcg
     1381 atgaacctct ctccggcgga agccctggaa agcatctcag caactctgga gattgcgcgg
     1441 gtaccaaaga caaaactcac ttctctgtga gtgaggagct catcagagtt cttctctgcc
     1501 acacagccgc atactccgac aatcagtttt gcatttctct tcttctgggc ctttagttgt
     1561 ccaagttttc cgtagagctt gtcttcggat ttctctctta cggcgcatgt gtttacgatg
     1621 actgcgtccg cttcttcttc actgttgact atactatggc ccgccagacg caagaggccc
     1681 atcatcgctt cagtatcgct tatattcatc tgacaaccaa aagttctgaa cgctaccttc
     1741 aaaagatcac ctctaacccg atattgattc tcagctaaat tctacatcat tgactgagtt
     1801 caggaaaccg cattatgtta ccattcaact gtagagttcc agaatatctt cgacagcgga
     1861 taatctaata attgccgatt actataaaac ctggaggtga aatatgtttg caagagctta
     1921 cgaaacggcc agctccttta ctcgtccagt cgtagttctg aacaggagag gtgatggaaa
     1981 agtaagtgcg acctgtggaa cgttcgttat cttgaacagt agaggctgga taattacagt
     2041 cgctcatctg tggcagtctt actttctctt caagaagcag caggcaaatc caaacgaaac
     2101 tcccgaagct caaaggataa ccaaccactc attctggtgg gggaaagacg gtataactct
     2161 caaggatata agaccgtttc cggaatacga tcttgttgtc ggaagacttg agccattcga
     2221 aacctcagag gtaaaggagt atccagaatt cattaaggcc gaagatatga gaataggtac
     2281 cagcctctgc cgtgttggct atccattctc cagaatcaaa gcgactttcg acgacaagca
     2341 gtcacgtttt cttatggact cgaagagcct tccgtctctc tatccgattg agggcatata
     2401 cacgaggtca gtgtccgcac cagcattcaa aggggcgaag aaaattgcca aattcataga
     2461 gacttcatct cctggacttc ttggacagag tggcggtcct attctggatg taacgggtcg
     2521 ggtctgggcc gttcagagtc gtaccgtgca tttgccgcta gggttcagtc caaaggcgag
     2581 aaaagagggc agagaaatcg aggagaatca atttctgaac gcaggttggg gggttcatat
     2641 tgagaacatc aatgaagtag ttcagagtgg tattgggtaa aatcatgaag atttgagaaa
     2701 caaatgtagt aaaatatgag ataggcaaac ccatgtttat tttcagatct caatactgct
     2761 cgatccatat caaatgatca gagggtgatc tgctttgtgc aaaaatgaaa atgctatggt
     2821 ttctgaacat ctcgttcaaa tgaatacgac tctagagaag gtgttttacc ttttgaaaag
     2881 aggcgttcaa aaggcaagcg aagagttcgt aaacagcgag aggaacgta
//
LOCUS       DAOQYI010000114         2875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_37357_length_2875_cov_78.441844, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000114 DAOQYI010000000
VERSION     DAOQYI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37357_length_2875_cov_78.441844"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..468
                     /locus_tag="PKL93_11200"
     CDS             217..468
                     /locus_tag="PKL93_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ71866.1"
                     /translation="MVVREYQGVKLDDLSAFRENSIKGVQYVNIEEYALKIGGLAETP
                     YFMNYTELQELQHVERLVTLHSVEGWTAKMLWEGIPLMN"
     gene            441..683
                     /locus_tag="PKL93_11205"
     CDS             441..683
                     /locus_tag="PKL93_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ71867.1"
                     /translation="MGRDSADELIEKADPDDRATNVIFKSTDGYTTILTLDYIRERNS
                     ILAGSLNGIGLPPAQGFPFIVVAEDRWGYKWARWVE"
     gene            complement(1351..2070)
                     /locus_tag="PKL93_11210"
     CDS             complement(1351..2070)
                     /locus_tag="PKL93_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71868.1"
                     /translation="MRNRSLDLNYKSFTRNRLTMFEYRKKPLLDQLERIENDMERLIP
                     QNDSEKLRRPGWVRSAERDVSQLLSKLSQFDQRRYADLQERMKETEERLDAVEIEEED
                     IELEAIADEIKHFEEFQKEYMEEVEKAKKKFEEEWDLTDIELKYSENDYIYKVMSKLE
                     ELRGRQTEINKGKALRELRKKKEVEGVLKFDNEEDKGTYVRQVAQRPVSSVEPPTKPE
                     TEEPPTIMRRRGGGRIKRDES"
     gene            complement(2401..2832)
                     /locus_tag="PKL93_11215"
     CDS             complement(2401..2832)
                     /locus_tag="PKL93_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thrombospondin type 3 repeat protein"
                     /protein_id="HNQ71869.1"
                     /translation="MKKVVLITIALLLIGGMTLAVGYGARQMKRVQDVAACENFVDED
                     GDGVNDNCPVDGEKVYMNRRGPAGKEVEGGFGTRTMQRLQDPTTCENFVDANEDGIND
                     NCPNGGKRLMENKSAQDGTGYRYGASGERGPGRGKGFGKTR"
BASE COUNT          702 a          715 c          621 g          837 t
ORIGIN      
        1 tcagaagact cgacggttgc tgccacccgt ttgactcatg aagaccttaa gcgaagtcat
       61 tagctcttga acaaagccat ctaatttgga acgaaactag atacgaggaa gagaatgttt
      121 tgaacttgcc ggttcttatt tcttactgga attatcgcag tggtatctat tgagtttgga
      181 gtcacattcg gcacaaatga tgggccctta gactaggtgg tggtaaggga gtaccagggc
      241 gtcaagctgg acgacctgag cgccttcaga gagaactcga taaagggcgt tcaatatgtg
      301 aatatcgaag aatacgcctt gaagataggt ggccttgcag aaacccctta tttcatgaac
      361 tacacggaat tgcaggagct tcagcatgta gaaaggctcg taacactgca ttctgtggag
      421 ggatggacgg cgaagatgct atgggaaggg attccgctga tgaattgatc gaaaaggccg
      481 acccggacga tagagcgaca aatgtgatat tcaagtcaac cgacggctac acaaccattc
      541 tgaccctcga ctatatcagg gaaaggaata gcatactggc cggcagtttg aacgggatcg
      601 ggctgccgcc ggctcagggc tttcccttca ttgtggtcgc cgaagacaga tggggctaca
      661 agtgggcgcg ctgggtcgag tagatcgaac tgtcagacga cgacaagtat agaggttact
      721 gggaagaatt cggatacagc aaagacggaa gccccgacaa accgatgctc gacaggtaga
      781 acgagaatta actctgacag acatctgttt cgtttcaatc tggccggata catccgaaga
      841 ccctgaatag acgatgcttc ttgacctgtg agtcgagtct gttctctttg gagcaagaag
      901 tgtccttcga gacaggattc gcggggataa ttaaccaaga gatgaaacta aatcagataa
      961 gagacttaag ttaagacgct ggttttcaag aaattgccgc tggaggtgaa actaacgccg
     1021 catcgcttag aaactacaca tgcgatgttt tttaagaaat cgtcttcttt tttcactcac
     1081 cttcgggaat cttccacatc gagcaacctc tcaactaaaa aagctctcaa ctcgaccgcc
     1141 tcaaagaaaa aagcgcagcc atcatgatag tcactccagc gcgcttcatc gcaaatcacc
     1201 catatacgaa atgaaaaaaa gtcaaaacaa accaattcga ttactcattc cttaaaatta
     1261 ttatacataa acgaacgcca aaatcaaaac aggcaaattt tatcggacaa ttcaagctga
     1321 gcttccgtga attcgccggt tatgcgagac tcaactttcg tcccgcttta ttcttccgcc
     1381 tccccttctt ctcattattg tgggcggttc ttccgtttcc ggcttagtcg ggggctctac
     1441 ggagctgacc ggtctctgcg cgacctgtct tacgtaagta cccttgtcct cttcattatc
     1501 gaatttaaga acgccttcga cctctttctt ctttcggagc tcccgcagtg ccttaccctt
     1561 gtttatttca gtttgtcttc cgcgaagctc ttcaagttta gacatcacct tatagatata
     1621 gtcattttcc gagtatttca gctcgatgtc tgttagatcc cattcttcct cgaatttctt
     1681 tttggccttt tctacttcct ccatatactc tttctggaac tcttcgaagt gcttgatttc
     1741 gtcggcgatc gcttccagct cgatatcttc ctcttcaatc tcaactgcat ctagtctctc
     1801 ttcggtttct ttcattcttt cctggagatc ggcatatctc ctttgatcga actggctcaa
     1861 cttgcttagc aactgagata catctctttc tgccgacctt acccaaccgg gcctccgcag
     1921 cttctcgctg tcgttctgag gtatgagtct ttccatatcg ttctcgattc tttccagctg
     1981 atctagcaac ggcttcttcc tgtactcaaa catcgtaagc ctgtttctgg tgaacgattt
     2041 gtaattaagg tccagagatc tgtttctcat agctgtgctc atcctcctcc attaagggat
     2101 tttttgtctt gattttcaga acggcggcag aaatgacctt cgctcaaaat gaaagatact
     2161 gatttctcat cgttagaaag attatataca tttgacaagt tgttccgttc ttctggagag
     2221 tcttgtcgag atgcttccga aagcgaaacg ttcatgcaac tttgttcgag acggataagt
     2281 tcttgaaggc atggttttct gggcagaagt atcttgaata aagcacttct gaagaattag
     2341 cttttgattg caagaaaaag ggcccatata gggccctatc tagatctttg gaggatgggg
     2401 ttatcttgtc tttccgaagc ctttaccgcg tccggggcct ctttcgccag aagcgccgta
     2461 tctgtatccc gttccgtcct gtgcgctctt gttttccata agtcttttgc cgccgttcgg
     2521 gcagttgtcg ttgatgccgt cttcatttgc gtccacaaag ttctcacagg ttgtaggatc
     2581 ctgaagcctc tgcattgttc ttgtgccgaa gccgccttca acttcttttc cggccggtcc
     2641 tcttctgttc atgtagacct tttcaccgtc aaccgggcag ttatcgttta ctccgtctcc
     2701 gtcttcgtcc acgaagttct cacatgccgc cacatcctga actctcttca tctgccttgc
     2761 accgtaacca acagccaaag tcattccgcc gatcaagaga agcgctatcg taatcagtac
     2821 tactttcttc atctgctttc acctccatct gattttcggt acattctcag tctaa
//
LOCUS       DAOQYI010000115         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_38673_length_2811_cov_64.887155, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000115 DAOQYI010000000
VERSION     DAOQYI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38673_length_2811_cov_64.887155"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(710..1816)
                     /locus_tag="PKL93_11220"
     CDS             complement(710..1816)
                     /locus_tag="PKL93_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HNQ71870.1"
                     /translation="MIDHSTRSIEIPKYIIVKKKATEELAPFLEIMRFKNVCVATQEN
                     LLDLMPDIQKAVNRNDYSIFYVEERPNLSHLMNRLDSVHDLHPDALVGIGGGSVLDTV
                     KFFAHILNIPLILVPTLASNDGICSPVVSLKLENGRSDSFRARMPFALFADTGVIYNA
                     PKKYLYAGLGDAISNVTAILDWEFATNRTGEQIDYFAKTLSVMSYKSLLNFKNANFAD
                     EDFVETLVNSLITSGISMEYAGNSRPASGSEHMLSHAADVIFDMAFLHGHQTGFYTCV
                     ILKAYGTELYEKVLEFLSDLGFFACAPEVLFKRDRFAEVVKLARSIRKRYTILNTLND
                     DQLLRIFDDTVSEIKYVISHANPEVPAGKSLRTL"
     gene            2120..2665
                     /locus_tag="PKL93_11225"
     CDS             2120..2665
                     /locus_tag="PKL93_11225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71871.1"
                     /translation="MDDYPEIRPIALKDLEDKALESFDRYQTTSEIKLKEPDGSFVYI
                     HDEFIDDWDSEKKVEVVQRLRSCMESGRFVAGAFLQNSIVGFASVDNSFFGSENQYLE
                     MPFMHVSSEMRGKGIGRKLFALCCENAKKRGAKKLYISTHPSIESQGFYESLGCLPAA
                     EINEEIYSREPFDIQLEFTLE"
BASE COUNT          800 a          577 c          676 g          758 t
ORIGIN      
        1 tgttcagggt catcccctaa ttccctcata ttgtcatccc gtaatgctcc tgtgcgggat
       61 ctcgtaccca tgaaccgcta tgcgctgaca agatcaaaac cggatcccga atcaagttcg
      121 ggatgactaa gtgagagacg cccgattagg cgagacgggt atgacagcgt ttgctgattc
      181 tgaaaacacg ataatttgtc atgctgaact tgtttcagca tcccgttcta agacacgcta
      241 tgcgctgaaa gaacaaaact gagtcccgaa acaagttcgg gatgacttgg agagggactt
      301 ccgcaaaccg atgtcgggat aacttcctca gttgcattga aacctgaatt cattgccggt
      361 tctcttcatc caggtcctga aagccgttgg gcgcttacaa ggagacgctc tccgctgcga
      421 agagccaccc ttcgctgcca gcgcaaaatc atggtgccgt tcttcgcttg ttagaaagaa
      481 agagtcatgg aatggaagat aactctggtc cctcaaagat cacagaccag atcccgagca
      541 aatttgggta atgaagtaag cgacatcgga acacgcatct tgccgcgtga gtaaaaaagg
      601 tgaaaaatga cgggagccgg tcaggctctc tttccggatt ttgcgcaagc acgatttgca
      661 gagacgaaag gctggttggg ggcgtaagtt agctattgta tcttagcagt tacagagtgc
      721 gaagtgattt tcctgcagga acttcaggat ttgcgtgaga tatgacgtat ttgatttccg
      781 agactgtgtc gtcgaagatt ctcaggagct gatcatcatt tagcgtattc agaattgtat
      841 atcgctttct tattgaacga gccaacttca ccacttctgc aaatctgtct ctcttaaata
      901 acacttcggg tgcacaggcg aaaaagccaa gatcggaaag aaattcaaga accttttcgt
      961 acagctcggt tccataggcc ttaaggatta cgcatgtgta gaagccagtc tggtgaccat
     1021 gaagaaaggc catgtcgaaa attacgtctg cagcatgaga tagcatatgc tcggaaccac
     1081 tagcaggtcg ggaatttcct gcgtactcca tggatattcc acttgtgatt aatgagttga
     1141 caagagtttc gacgaaatcc tcatcagcga aattagcgtt cttgaagttg agaagacttt
     1201 tatagctcat aactgacaac gtctttgcaa agtagtcaat ttgctcgccg gtcctattgg
     1261 ttgcgaactc ccagtcgaga atggctgtga catttgagat tgcgtcgccc agcccagcat
     1321 atagatactt ctttggcgca ttgtaaatga cacctgtatc tgcgaacagg gcgaacggca
     1381 ttctcgctct gaagctgtcg cttctaccgt tttcgagctt cagagacaca accggggagc
     1441 aaatcccatc gtttgaggca agagtgggaa ctaaaatcaa cggaatgttg agtatgtgag
     1501 cgaaaaactt gacagtatca agaaccgagc ctcctcctat tccgacgagc gcatcggggt
     1561 gtagatcgtg aactgaatca agtctgttca ttagatgact gagattcggt ctctcttcta
     1621 cgtagaaaat ggaatagtca ttgcgattaa ctgccttttg aatatcaggc attagatcta
     1681 gcagattttc ctgggttgca acacaaacat tcttgaacct cattatttca agaaaaggag
     1741 ccagttcttc cgttgctttt ttcttgacaa taatgtattt gggtatctct atagatctcg
     1801 tcgaatggtc gatcaattca tcacctttag tattggataa tgagaagatc aaatgtgcat
     1861 gagcttccag aaaaattata acagtcggta tagaaaaacg cgatttcgat gatttttcct
     1921 tttgctaaag aagctcctca taagtggaaa aaggaggcct gaccaacgca tttatgaagc
     1981 ctcgacactc ttagaaattc tgcaagtgca taaaactaat tgtatcggat ttttatagaa
     2041 ttttgccttc accagcgtat acaattgagt gggcacaatt gtgcccctga tgccctatca
     2101 gatgaaagga gagagtcctt tggatgatta tccggaaata agaccaatag cattaaagga
     2161 tctagaagat aaggctctgg agagttttga taggtatcag actacgagcg aaatcaagct
     2221 taaggagccg gatgggagtt ttgtctatat tcatgatgag ttcatagatg attgggattc
     2281 cgagaaaaag gttgaagtgg tacagcgttt aagaagctgt atggaatctg gacgattcgt
     2341 tgctggtgcc tttctccaga attcaatcgt aggctttgca tctgtggata actctttctt
     2401 cggaagcgag aatcagtatt tagagatgcc gtttatgcac gtctcaagcg agatgagagg
     2461 gaaaggcatt ggcagaaaac tcttcgcgtt gtgctgcgaa aacgctaaga aaagaggcgc
     2521 aaagaaattg tatatttcaa cacatccatc aatagagagc cagggtttct atgaatcttt
     2581 gggatgcctt cctgctgccg aaataaacga ggagatctat tcgcgagaac ccttcgatat
     2641 acagcttgaa ttcactttag agtgattagt ttgtgagata tttgaactcc gcgcaggtca
     2701 ttaaaaagta tcgagatata gttgagtgca tggagcggct aatcgacaaa gattcgacgt
     2761 ccattggaat agggatcctg accaggagca ttgtcaggat gacgtttagg g
//
LOCUS       DAOQYI010000116         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_39962_length_2751_cov_71.817136, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000116 DAOQYI010000000
VERSION     DAOQYI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39962_length_2751_cov_71.817136"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..734
                     /locus_tag="PKL93_11230"
     CDS             90..734
                     /locus_tag="PKL93_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ71872.1"
                     /translation="MAKLLPDNITKQVKEIFSSMEGSVKALLFKSEKNCDYCSTAEQM
                     LQELSEIESKISYEVHDLDSEMAEKYGIELAPSMVLLTPEGDDKGVRFLGIPAGHEFG
                     TLLQDIISFSKGAISDLSKDSIQKIEKINSPVDIKVFVTTTCPYCPKAVLTAHNIAMA
                     NKNITAYMVEANEFQELSRKYGVSSVPQIVINDKVTFVGAYPEGQFVDQVMKAV"
     gene            746..1705
                     /gene="trxB"
                     /locus_tag="PKL93_11235"
     CDS             746..1705
                     /gene="trxB"
                     /locus_tag="PKL93_11235"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNQ71873.1"
                     /translation="MSFFELGSAKKSELKDFYDVTIIGAGPGGITAGIYAVQAGLNPL
                     IIEKALDGGQMNNTQTVENWTGFASISGMELSEKMADHAREFDVPFTYAEVVDIDGEG
                     QNKEVILDNGKKIKSRVIVIATGSNPRKLGVMGEEDFKGRGVSYCATCDGHFFADKHI
                     AVIGGGNSALDESLYLSKMVSKITVVQNLPKLTADKLLQKKLNDTGKVEYIYSSVVDS
                     IEGDSQVRKINIKNVDSGEITSLEVDGIFVFIGLIPNTQFLKGKLEMNDFGYIKTDDF
                     METKTKGVYAIGDVREKEVRQIVTAAADGAIAIYHAARHYFSE"
     gene            complement(1763..2707)
                     /locus_tag="PKL93_11240"
     CDS             complement(1763..2707)
                     /locus_tag="PKL93_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNQ71874.1"
                     /translation="MRRVWLTVMMVVGMLLVSPVLFSGEIRTIFVTAPASFPDRSKLE
                     AGIAALRNVGYRVVVGDSCQISLSPREKANELNKAFANPEYDAIITARGGYGSYNLLD
                     WLDWEVIASNPKPLVGYSDITALLLSVYFKTGQTTYHGPMVAVELGTDSQSVQNIAAI
                     FNGEKTICFNYQSIPVIAGKMTGRLIPANLSLFQALQGTEYLGSLKDAILVLEDVSET
                     KESLERMIWNIAHLDEFSSLRGVVFAGFTEIKNGDLDDIKRMIHDYFLGKEIPVWIGL
                     PSYHGDFPKVVLPVGQWVEIDMEKGTIEILPVPEAKIK"
BASE COUNT          817 a          561 c          650 g          723 t
ORIGIN      
        1 tgtaaacaag ttgtcaaagg aagagtaata taaaagtgtt accatacccc tagtgggtat
       61 aaaaatatta gtccttggga gggatataag tggcgaagct tctgcccgac aacataacca
      121 agcaagtaaa ggaaatcttc agcagcatgg agggctctgt aaaggcactt ttgttcaaga
      181 gcgagaaaaa ttgtgattac tgttcaactg cagaacaaat gctgcaagag ctttccgaaa
      241 tcgaaagcaa gattagttac gaagttcatg atcttgattc agaaatggcg gagaaatatg
      301 gtatagagct tgcacccagc atggtacttc tcactccgga aggtgacgac aagggtgtca
      361 ggttccttgg aatacctgca ggacatgagt ttggaacgct tctgcaggat atcatttcct
      421 tttccaaggg tgcgatttct gatctctcaa aggattcgat tcagaagatc gaaaagataa
      481 actctccggt ggatataaag gtctttgtta ccaccacgtg cccttactgt ccaaaagcag
      541 ttcttacagc tcataacatc gcaatggcga acaagaacat aacggcttat atggttgagg
      601 caaacgagtt tcaggaattg tcgaggaaat atggggtctc atcggtcccg caaatcgtga
      661 taaatgacaa ggtaaccttt gtgggagcct accctgaggg tcagtttgta gatcaagtaa
      721 tgaaggcggt ttgaggaggt tcaaaatgtc attcttcgaa cttggtagcg ctaagaaatc
      781 cgaactgaag gacttttacg atgtgacgat cattggagcc ggtcctgggg gcattacggc
      841 tggtatatat gccgtccagg caggtttgaa tcccttaatt attgaaaagg cacttgatgg
      901 cggacagatg aacaatactc agaccgtcga aaactggacg ggtttcgcaa gtattagcgg
      961 catggaattg agtgagaaga tggctgacca tgctagagaa tttgatgtcc ccttcacata
     1021 tgcagaagtt gtagatatcg atggcgaagg tcagaataaa gaagttatcc ttgataatgg
     1081 taaaaagatc aaatccagag taatagttat tgctaccggt tccaatccca gaaagcttgg
     1141 agtaatgggt gaagaagatt tcaaaggcag aggtgttagc tattgtgcaa cgtgtgatgg
     1201 tcacttcttc gcagacaagc acattgcggt gattggaggc ggaaacagcg ctctggatga
     1261 gtcactttat ctgagcaaaa tggttagtaa aataaccgtt gttcaaaacc tgccgaagtt
     1321 gactgcagac aaactactac agaagaagct taacgatacg ggaaaggtcg agtacatcta
     1381 cagttcagta gtggattcca ttgagggcga ttcacaggtc agaaagataa acattaagaa
     1441 tgttgacagt ggcgagatta cttctctaga ggttgatgga atctttgtgt tcattggttt
     1501 gattcctaac acacagtttc tgaagggcaa actcgagatg aatgattttg gatacatcaa
     1561 gacggacgac ttcatggaaa cgaaaacaaa aggggtttac gccataggcg acgtaagaga
     1621 aaaggaagtc agacagatag taaccgcggc agcagatgga gcaattgcca tctatcatgc
     1681 cgctagacac tacttcagtg aatgatcagt aaagaataga acaaatatag agggagaaga
     1741 gcgttttccg acgctcttct ctttatttga tctttgcttc aggaaccgga agtatttcga
     1801 tcgtgccctt ctccatgtcg atttcaaccc actggccaac cggaagaacg actttcggga
     1861 agtccccatg ataactcggt agaccaatcc aaacaggtat ttcctttccc aggaaatagt
     1921 cgtggatcat tcgttttata tcatcgaggt ctccattttt tatttctgtg aatccagcga
     1981 ataccacacc tcttaaagat gaaaattcgt caagatgtgc aatattccag atcattcttt
     2041 ccagcgactc tttggtctcg ctcacatctt caagaacgag aattgcatct tttagtgaac
     2101 ccaaatactc tgtcccctga agagcctgga atagagaaag attggccggg atcaacctgc
     2161 cagtcatctt cccggcgatc acaggaattg actggtagtt gaagcagatc gttttttcac
     2221 cgttgaaaat ggctgcaatg ttctgtaccg attgagaatc tgttcctagt tcaaccgcaa
     2281 ccattgggcc atgatatgtg gtctggcctg tcttgaaata gactgaaaga agcagtgctg
     2341 tgatgtcgct gtaaccaaca agaggttttg gattcgaagc tattacctcc caatcgagcc
     2401 agtcgagaag gttgtatgaa ccataaccgc cccttgcagt tatgattgcg tcgtattcag
     2461 ggttagcaaa ggctttgttg agttcatttg ctttttctct cggggaaagt gaaatctggc
     2521 aactatctcc gacgacgacc ctgtaaccca cgttcctaag tgctgcgatg cccgcctcca
     2581 gctttgatct gtctggaaaa cttgcaggcg cagtgacgaa aatcgttcgg atctctccag
     2641 aaaacaatac aggtgaaact aaaagcattc caacaaccat cataactgtc aaccataccc
     2701 ttctcatgaa atcctccaag ttgatctgtc tactatagtt tgccattcat g
//
LOCUS       DAOQYI010000117         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_40386_length_2732_cov_70.439671, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000117 DAOQYI010000000
VERSION     DAOQYI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40386_length_2732_cov_70.439671"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKL93_11245"
     CDS             <1..665
                     /locus_tag="PKL93_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ71875.1"
                     /translation="VETLEVVGIFDLGVASLNRNWLISTIDTAQSVGDLGDAVTSIEM
                     QVSEVFRADENASLISEALRNPSLQVTDWKSQNEDLLSGLNGQSISSIMIQVFVIIAV
                     SLGIASVLAVTVVQKSRQIGILKAMGLRDSTTSFVFLFQGLALGVVGAVVGIAFGLLL
                     IIMFSTFAVGPDGEPILSISLNYGFVMLSAIIAISASTIAAMVPARRSSKLSPVEVIR
                     NG"
     gene            658..1344
                     /locus_tag="PKL93_11250"
     CDS             658..1344
                     /locus_tag="PKL93_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71876.1"
                     /translation="MADIIKLEEIKKEYGTVVKTEVLHGINLAIEESSFISIVGQSGS
                     GKSTLMNIIGTLDKPSSGEVTISGRKTNKMNKNELAKVRNETIGFIFQFHYLLPEFTA
                     FENVILPYRIKGLKPTKEVVERARELMDIVEISMVRNNLAPNMSGGQQQRTAIARALI
                     NNPKIILADEPTGNLDSDTSSKVFNLMRSLNKKYGTTFVIITHDRRIAEETDRIIEIK
                     DGNIVNDITR"
     gene            complement(1388..1972)
                     /locus_tag="PKL93_11255"
     CDS             complement(1388..1972)
                     /locus_tag="PKL93_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNQ71877.1"
                     /translation="MEEFLSGDLFKWVIMPLIIFFARIVDVSLGTTRIIMVSRGKKEI
                     ASLIGFFEVIVWLLVASKVIQSVDNVLYILAYAGGFAAGSYIGILIDERLAMGTVSIR
                     LITSVEPTELINKLCQAGFGVTKIDAQGSRGKAYIIYSIINRKEVSEFERIAQECVPK
                     AFMSVEDIRKVREGVFLPRDTLRIRRESPLRKSK"
BASE COUNT          777 a          609 c          586 g          760 t
ORIGIN      
        1 cagtcgaaac cctcgaagtc gtcggcattt ttgatctggg agttgcaagt ctcaatagga
       61 actggctgat ctcaacgatc gatactgctc aatccgtggg ggatctgggc gatgcggtca
      121 cttcgatcga aatgcaggtg agcgaggttt tcagagccga tgaaaacgct tcactaattt
      181 cggaagctct tcggaatcct agtctacaag ttacagattg gaagtcccag aatgaggacc
      241 tcctttccgg gctgaacgga cagagtattt caagtataat gattcaagtc ttcgtaatta
      301 ttgccgtttc gcttggaata gcaagtgtcc tggcagtaac tgtagtccag aaatcgagac
      361 agataggaat actaaaagca atgggtttga gagattctac aacaagcttc gtcttcctat
      421 ttcaggggct cgcactggga gttgtgggtg ctgttgttgg aatagccttt ggcttattgc
      481 ttataataat gttttctacc tttgccgttg gaccagacgg agaaccaata ttaagcatct
      541 ctcttaacta cggatttgta atgctttctg cgatcatagc cataagtgct tcgaccattg
      601 cagccatggt tcctgcaaga agatcctcca aactcagtcc ggttgaggtg ataagaaatg
      661 gctgatatta taaagcttga agagataaag aaagaatacg gaaccgttgt caaaaccgaa
      721 gtgctgcatg gaataaatct tgcgatcgaa gaatcttctt tcatctcgat agtcggccaa
      781 tctggaagcg gaaagtctac actgatgaat attattggaa ctcttgataa gccatctagc
      841 ggagaagtaa cgatttccgg caggaagaca aacaaaatga ataagaatga actcgcaaaa
      901 gtaagaaacg aaacaatagg atttatcttc cagtttcatt acctccttcc ggaattcact
      961 gcttttgaga acgtaattct cccgtacaga ataaagggtc tcaaaccaac gaaagaagta
     1021 gtagaaagag cgagagagct tatggatatt gttgaaatca gcatggtcag gaacaatctt
     1081 gcgccaaata tgtccggagg tcaacagcag agaacggcaa tcgcgagggc actcataaat
     1141 aatccgaaaa tcatcctcgc ggacgagccg acaggtaatc tcgattcaga tacttcgtca
     1201 aaggtcttca atctcatgcg cagccttaac aagaaatacg gaacaacctt cgtgatcatc
     1261 actcatgaca gaaggatcgc agaagaaact gacagaataa tcgaaataaa agacggtaac
     1321 atcgttaacg acataacaag atgagggccg aacccttatc tctttctcgt agtaatccat
     1381 acagaggtca cttcgatttt cttaaaggcg attctcttct tattcgcaat gtatctcttg
     1441 gcaagaagac tccttcccta accttccgga tatcttcaac agacataaac gcctttggta
     1501 cgcattcttg agctattctt tcaaactctg agacttcctt cctgtttatt atactgtaga
     1561 ttatgtaagc ctttccgcga gagccttgcg catctatctt tgtcacccca aatcccgcct
     1621 ggcagagttt attaatcagt tcagtcggtt caactgatgt gattaacctt atagagacag
     1681 tacccattgc cagtctctca tctatcaaga tccctatgta gctaccggca gcgaaaccgc
     1741 ctgcataagc aagaatgtaa agtacgttgt caacactctg gataaccttg ctggcaacga
     1801 gcaaccatac aataacttca aagaaaccga tcaggctggc aatttccttc ttccctctag
     1861 aaaccattat gattctcgtt gtccctagag agacatcaac gattcttgcg aagaagatta
     1921 tgaggggcat tatcacccat ttaaagagat ctccactgag aaattcttcc attagtaacc
     1981 ctcctaccgc atttcaaaat aattatctgc ccgtccgtgc cgattataag atattagtcg
     2041 agatgtgggg tgattgagag attgccaaag ttacggttaa cctccattaa ctctcattat
     2101 tacacataag tcaggaaatt taatcccgct aattggcttt ttcttgcgcg aatttgtatt
     2161 tgcgtactaa tgatcctgtt tctttaatcc tcacatgagg gagctcatca ttacccttga
     2221 gtctgggagt taaattccac taatttgctg tttcttgcat gagtttgttt tttgctccga
     2281 atgatcccca tcttaaatcc acctttgagg gagcagggcc acgtagtggc ggagggtgtg
     2341 agtccagcta aatctggcca actacatagt ttggaagaag cgcttccgaa ggcggaagtt
     2401 acttccggca tcggaagtat tttccaggac cttcgtggtc tttctggact ccgtactttt
     2461 gcgttgatgt tcgatcctgt taacttcttg gctttgtaga ccggttgcaa gttctcggtt
     2521 ccaagttgct ggctgcggcc atgaaaccga cacaatctgt catctgaact tgtttcagaa
     2581 tcaagttcct aagagacgct atacgctgaa agaccagttg caagttctcg gttccaagtt
     2641 gcaggttaaa gacccgtagg caaaaacgcc acagcccgtc attctgaact tgtttcagaa
     2701 tctagttcct aagtgccgct gagcactgtg aa
//
LOCUS       DAOQYI010000118         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_41107_length_2703_cov_83.545317, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000118 DAOQYI010000000
VERSION     DAOQYI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41107_length_2703_cov_83.545317"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1186)
                     /locus_tag="PKL93_11260"
     CDS             complement(287..1186)
                     /locus_tag="PKL93_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HNQ71878.1"
                     /translation="MIISASRRTDIPAFYSDWIIERIRSGFALVRNPFNPRQVSKVTL
                     NPEAVDCLVFWTKDPKNMLDKLSYLKDYCYYFQFTITPYDRSVEPGLRDKGNIIETFK
                     KLADRIGRERVIWRYDPIIVSEQFNLAYHFRAFEKMVDLLQGHTEKCVISFLDYYKKI
                     SSGLRRLGLRRPERDEIRDVVRFFAGKAKDAGIRIETCSEEEDLDEFGITHGSCIDGN
                     LINRLTGRNKQYSKDRYQRSACRCVESVDIGSYNTCLHRCSYCYANFSQKMIYRNYGR
                     YDSKSPILCSRLDAGDTITERKE"
     gene            1342..1839
                     /locus_tag="PKL93_11265"
     CDS             1342..1839
                     /locus_tag="PKL93_11265"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ71879.1"
                     /translation="MSRYLETMRAFFDRGADSCESHMKNNVKESAAFYREMARLVPVT
                     TGIRILYLGCGTGLEIDEIFKLNSTARVTGIDLSEKILEILRSKYEGKKGTTNLLPVP
                     ISKSNCRWRDSTLPFRFNRCTTSDKNKSYRSTGRFSQSSGNGTYIEADYVVGTAIEEM
                     ELLNR"
     gene            complement(2091..>2703)
                     /locus_tag="PKL93_11270"
     CDS             complement(2091..>2703)
                     /locus_tag="PKL93_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNQ71880.1"
                     /translation="HGVDALRTYLMFMGTFLEGGDFRDSGMDAMRRFLNRVWDIATMP
                     EGRSSTELKIKMSETVEKVEYSIKNIKPNTAIAAIMEFVNEASKEASIERQLVIDMAK
                     LLSPFAPFVCEEIYSMKGGKKKTILDDGFPYGYEKYASMAKTELPVQITGKMRGKVVL
                     PQNASQEEAMEAVMADEKLSKMLEGKSIRKIIYVQDKIINIII"
BASE COUNT          759 a          621 c          558 g          765 t
ORIGIN      
        1 gtcattctga acttgtttca gaatctagtt cctaagtgcc gctgagcact gtgaagaacc
       61 gtctttggat aagatcaaaa ccaagatccc gaaacaagtt cgggatgaca aagtgagaga
      121 aacgcgagaa ggcaagactg aaaagacgcg agattttagc tgattctgca acgaagggac
      181 aagatcactg agctccagaa aagatgatgt ggctaataat gaacccatta aaggcaaaaa
      241 gacactaaat ctagatcttg gaacctagaa cgccttcagg aatcggctac tcttttcttt
      301 ctgttatggt gtctcctgcg tctagacgac tgcagagaat cggagacttt gaatcgtacc
      361 tcccgtaatt tctatagatc atcttctggc tgaagttcgc gtagcagtaa ctgcagcgat
      421 gaagacaggt attatatgaa ccgatgtcaa cgctttcaac gcatctacat gcagatcttt
      481 gatatctgtc ttttgagtac tgtttgtttc tgcccgtaag cctgtttata agattgccgt
      541 caatacaact gccatgggtt attccgaatt cgtcgaggtc ttcctcttca gaacacgtct
      601 cgatacgaat ccccgcgtcc tttgctttcc cggcaaaaaa ccgaacaaca tctctgattt
      661 catctctttc cggtcttctt aaacctaatc tcctcagacc tgaagagatt ttcttgtagt
      721 aatctagaaa actaattaca cacttctccg tgtgcccttg aagcagatcg accatcttct
      781 cgaaggctct aaaatggtaa gctaaattga actgctccga aactataatc ggatcatatc
      841 gccagattac tctttcccta ccgattctat ccgccagctt cttgaaagtc tcaatgatgt
      901 ttcccttgtc gcgtagtccg ggctctacag atctatcata gggtgtgatt gtgaactgga
      961 aatagtagca gtagtctttc aagtaagaca gtttgtcgag catgttctta gggtctttgg
     1021 tccagaaaac aagacaatct actgcctccg gattcaaagt taccttactt acctgtctgg
     1081 gattgaaagg attcctgacc agagcgaaac cgcttctgat tctttcaata atccaatcgg
     1141 aatagaatgc cgggatatcc gtcctcctgc ttgcgctgat tatcaatgtt gtctcctttc
     1201 gggcaaaagc tcatttagac ttccgtgaaa acccccacct taatagttac ggcaaactcg
     1261 gcttcatact cgttcggcac ttctataata taagaaagca gattgacctg ccaaagaagc
     1321 aaaaaaacgg agcgagagaa aatgagccgt tacttggaaa caatgagagc tttcttcgat
     1381 agaggagccg attcatgcga atcccacatg aaaaacaatg tcaaggaatc ggctgcattc
     1441 tacagagaaa tggcaaggct agttccagtt actacaggaa tcaggatact ttatctcggt
     1501 tgcgggacgg gcctggaaat tgacgagata ttcaaattga attccacggc cagggtaacc
     1561 ggcatcgacc tatcagagaa gatcctcgaa attcttagaa gcaaatacga aggcaagaag
     1621 ggaaccacga atttgttgcc ggttcctatt tcgaagtcga attgccggtg gagggattcg
     1681 acgttgccat ttcggttcaa tcgatgcacc acttcggaca agaacaaaag ctatcgctct
     1741 acaggaaggt tttcgcagtc tagcggtaat ggaacatata tcgaagcgga ttacgttgtc
     1801 ggtactgcga ttgaggagat ggagttgctc aacagataag gttctctgaa aagtgagaat
     1861 aacttacctg acggtttcta tcacttcgat ttgccacttt ccacagataa tcaacaagca
     1921 gttcaagaat aggctgggtt caggcctgta agacaccatg caagatacgg gaataccagt
     1981 atttttgttt cgcgaaagcc cgtatcaaaa tgaagatgaa tctatggaag acaatggaga
     2041 acaactggct ctccattttt gtattcagaa cagaatattg tttactgcgt tcaaataatt
     2101 atgttgatta tcttgtcttg aacgtagatg atctttctta ttgactttcc ttcaagcatt
     2161 tttgagagtt tttcatcagc cattaccgct tccattgcct cttcctggga tgcgttctga
     2221 ggaagcacga cttttcccct catctttcca gtgatctgga caggcaattc cgtcttggcc
     2281 atagatgcgt acttctcata accatatggg aatccatcgt caagaatcgt cttctttttt
     2341 ccacccttca tactgtaaat ctcttcacag acaaacggtg caaatggaga aagaagcttt
     2401 gccatatcta taacaagctg tctttcgata gaagcttctt tcgaagcttc attaacaaac
     2461 tccatgatag cggcaattgc cgtgttcggc ttgatattct tgattgaata ctctactttc
     2521 tctacagtct ctgacatctt tatcttcaac tcggtagaac ttctcccttc gggcatagtg
     2581 gcgatgtccc atacacgatt gagaaacctc ctcatagcgt ccatcccgct gtccctgaag
     2641 tctcctcctt caagaaacgt acccatgaac atcagatatg tcctgagggc atctactcca
     2701 tgg
//
LOCUS       DAOQYI010000119         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_42410_length_2647_cov_69.817515, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000119 DAOQYI010000000
VERSION     DAOQYI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42410_length_2647_cov_69.817515"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            473..718
                     /locus_tag="PKL93_11275"
     CDS             473..718
                     /locus_tag="PKL93_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71881.1"
                     /translation="MRRKKGSPARDPKRYKGYFSRRDSSTGRFVSSYRDRFRNISSEE
                     RVHREYHRIMNEIYDRNRDSYPNVVEERRELIESLKI"
     gene            699..1439
                     /locus_tag="PKL93_11280"
     CDS             699..1439
                     /locus_tag="PKL93_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71882.1"
                     /translation="MRVSRSNTTSIFRVKKQRNFVAIDKSFLSDPSLSCKAKGLLAFF
                     LSKPDDWEFRLSEIRRNFKDGKESIRRGIEELQKAGYVKKVKLRGTKGKFDGVRYLIY
                     ETPYLSEVHPQTDFPASGKPKADNPAAENPSLLNNDITKDTSYRAGTSEDISKWGKND
                     FIKRTASLIKERLEIEDNYKLAAALIGDSDRQIDWYFPMWLVEHFTEAINRADDKKKY
                     LFSLLKKPELWKLFMSFLESESFNGRGL"
     gene            1443..>2647
                     /locus_tag="PKL93_11285"
     CDS             1443..>2647
                     /locus_tag="PKL93_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ71883.1"
                     /translation="MMILDVRSERQVLKALIHYINLRCRIDEIDRRFLSQESQIIYDI
                     ISKKRDKDPTLILMEMYNRVERFDVDDVELVDENHANNLIDYFNETLRNKKFRFLLNQ
                     ISRDMGQGSMGIEAATERIMSFLHESSSGFKYVDMKQANMLLKQFHTDVWANKSKKLH
                     WPFRYFNTVLNPLRGKELVVIAGETATGKTSFMLNTADRWAAEGKTIGYISLEIDVVD
                     LKNRMHQKDFDINLSAESDKLSEEGRLLLNDAIDRTMDQKMYFCDSGVRSVHEIIAGI
                     KLMNMLHQFDVVFIDYLQLMSCPGKQSKDAEIGEIMGSLKALAMDIKVPIIVASQLNR
                     EVGKNDKGIPKLSNLRESGTIEHSADVVILIYRPILYNKSQSLNEYHAIIGKQRNGKT
                     GTIYMDFNMT"
BASE COUNT          838 a          499 c          657 g          653 t
ORIGIN      
        1 catctataaa atagtgactg ataaacgaag accggtcttc ggattatcaa gaagagtctc
       61 ttcgattttc tgaactctca agaggagtgg caagagaatt cggctcagcg gattctatct
      121 ttttcatggg aatttggtaa taagttctgc ttctaaaaag cagctccgct tgttggatga
      181 tagatattct caaaacggat tcaggttttg cttaaggagg tgattttgaa tgcagctctg
      241 cgtgggaaac aactgaacac tatatagata tggagggaga aaatacggaa tttgactgcg
      301 ttattctttt cgagcactcg aagggccttt gaagcgaaca atcttaacaa aacttaaaga
      361 atatttaagt gcttggaatc agggtttctt tgcgattatg atatgcccag tcggtgtttc
      421 ctgttccgat tccgtatgaa aaatctcaaa ccgaaagaag gtgggcaagt tggtgagaag
      481 aaaaaaagga tctcctgcaa gagatccgaa aaggtacaaa ggatattttt cccgaagaga
      541 ttctagcaca ggaagattcg ttagttccta tcgtgatcgt ttccgcaaca tttcctctga
      601 ggaaagagtt cacagggagt accacaggat tatgaacgag atctacgaca ggaatcgcga
      661 ttcctatccg aatgtagttg aagaaaggag agaattaatt gagagtctca agatctaata
      721 ccacatctat tttcagagtg aagaagcaaa gaaatttcgt agcgatcgac aagagctttc
      781 tgagcgatcc ttctctttca tgcaaggcga aaggacttct cgccttcttt ctttccaaac
      841 ccgatgactg ggagtttcgg ctctccgaga tcaggaggaa ttttaaggat ggaaaggaga
      901 gtattagaag gggaattgaa gaactccaga aggccggtta tgtaaaaaag gtgaagttac
      961 gaggaacgaa gggcaagttc gacggggtga ggtacctgat ttacgaaact ccttatttga
     1021 gcgaggttca cccgcaaacc gatttcccgg cttcgggaaa accaaaagcg gataatccgg
     1081 cggcggaaaa cccgtcacta ctaaataatg atattactaa ggatacttcg tatcgtgcag
     1141 gcacgagcga agatatatct aagtggggaa agaatgactt catcaagaga actgcaagtc
     1201 tgataaaaga gaggctggaa atcgaggaca attacaaact tgcagctgcg ctaataggag
     1261 attcagatcg acagatcgac tggtatttcc ccatgtggct tgtcgaacat ttcaccgagg
     1321 caataaacag ggcagatgat aagaaaaagt accttttctc gctcctgaag aaaccagagt
     1381 tatggaagct ttttatgagc tttctcgaga gcgagtcgtt caatggaagg gggttatgag
     1441 agatgatgat tctcgatgtc aggtccgaaa ggcaagtgct aaaggctttg atacactaca
     1501 taaatcttag gtgtaggatt gacgagatag atcgaaggtt tctttcccag gaatcgcaga
     1561 ttatctacga cattatttca aaaaagagag acaaagatcc cactttgatc ctgatggaaa
     1621 tgtacaacag ggtggaacgc tttgatgtcg atgatgttga actggtagat gaaaaccatg
     1681 ccaacaatct gatcgactac ttcaatgaga ctttaaggaa caagaaattc aggtttctgc
     1741 taaaccaaat ctctcgtgat atgggacaag gcagcatggg aattgaggct gcaaccgaaa
     1801 gaatcatgtc cttccttcat gagagctcgt ctggattcaa gtacgtggac atgaagcagg
     1861 caaatatgct tctaaagcag tttcacacgg atgtctgggc gaacaaaagc aagaagcttc
     1921 attggccgtt cagatatttc aacacggttt tgaaccctct taggggtaag gaacttgtgg
     1981 taatagcagg agagacagca acgggaaaga cttcctttat gctgaatact gcggatcgct
     2041 gggctgccga gggaaagact ataggatata tctctctgga aatcgatgta gttgatctta
     2101 agaacagaat gcaccagaag gatttcgata taaacctcag tgcagagtcg gacaagctga
     2161 gcgaagaagg acggttgcta ctgaacgatg cgatcgacag gacgatggat cagaaaatgt
     2221 atttctgtga cagcggggtg agatctgttc acgagatcat agcaggcatt aagctgatga
     2281 atatgcttca tcaattcgat gttgtcttca tagattatct ccagctcatg agctgcccgg
     2341 gaaagcagtc gaaggacgca gagatcggcg agataatggg gtcgctgaag gctcttgcca
     2401 tggatatcaa agtcccgatc atagtcgcct cgcaactgaa cagggaagtc ggcaaaaacg
     2461 acaaggggat tccaaagctc tcaaatcttc gagaatctgg aacgatagaa cacagcgccg
     2521 atgtagtaat tcttatctac agacctattc tgtacaacaa gagccagtcg ttgaacgaat
     2581 atcatgcgat aatcggcaaa caaagaaacg gaaagacagg cactatctac atggacttca
     2641 acatgac
//
LOCUS       DAOQYI010000120         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_43180_length_2615_cov_55.388281, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000120 DAOQYI010000000
VERSION     DAOQYI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43180_length_2615_cov_55.388281"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKL93_11290"
     CDS             <1..422
                     /locus_tag="PKL93_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71884.1"
                     /translation="ASKLLQNFKLSDAIKDPVSTYSHGMKQKLSLIAALIHNPDLWIL
                     DEPIVGLDPESAFILKQMMRNHAKAGNTVFFSTHVMEVAERICDRIGIINKGKLEFVG
                     TVEELRKLRGKGSLEELFLEVTGSETENGDFAYLDAD"
     gene            379..2049
                     /locus_tag="PKL93_11295"
     CDS             379..2049
                     /locus_tag="PKL93_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71885.1"
                     /translation="MRPKMVISLTSTLINAHYNIPRSLYHFKKRERLWEPALIIGVVL
                     LMVFSLGPLYMSLLNTMYDQYDAAGMRQLFLAGPFLIANLFGLIMGVFLMISTFFFGD
                     DMSVLIPLPLKSTEVLMSKFLVVLMDLMVISIAVILPAYILYGIRSGAGISYWIFMAI
                     IFLLSQVLPIMLSAIIVLPLSRLFRFKRYRDSIVYLISALVIIGSLVFVSLTSRVDPN
                     MSAEEFARIFSSEDAILNKTAGAYPPTILVTKALSKPFLEGLLWLLAFIGLHAAALGI
                     FLFFGHKFYYSVYSSLQENFAKRSKLKEGEIAGLMGLQQTKFSALYKREWWYFLKVPA
                     FAFNGFGNVLVFPILLVIAAVAGQTDQLGQMLDQFQQYKPLFVPVGALVAAVAGGING
                     LASSMFSREGDLIRELKVLPVDVNEIVKVKFLHIETLSLIGPAFGAVALSLILGLSFL
                     EALVVFVVGALTLTFLNILQMIIDSIKPILEWENPQRAMKQNVNVALSIPVVFGYVGG
                     LGYLAFLLKDNISGTIMTIILTAIALVGIVFTWPILIKKANRLFARDL"
BASE COUNT          674 a          584 c          637 g          720 t
ORIGIN      
        1 gggcatctaa gcttctgcag aatttcaagc tctcagatgc aatcaaagat cccgtctcca
       61 cttactctca tgggatgaaa cagaagcttt cgctgattgc cgcactgatc cacaatcccg
      121 atctctggat actcgacgaa ccgatagtcg gcctcgatcc ggaatcggca ttcattctca
      181 aacaaatgat gagaaaccac gcgaaggcag gaaatacggt attcttctcc actcatgtca
      241 tggaagtggc agaaagaatt tgcgacagga tagggataat aaacaaagga aagcttgagt
      301 ttgtaggcac cgttgaagag cttaggaagc tcagaggaaa gggaagcctt gaagaactct
      361 tcctggaggt gacgggtagt gagaccgaaa atggtgattt cgcttacctc gacgctgatt
      421 aacgctcact acaacatacc tagatctctc tatcatttca agaaaagaga aagactctgg
      481 gaacctgcat taataatagg cgtggtactc ttgatggtct tttctcttgg gcctctttac
      541 atgagtctgc tgaacacaat gtacgatcag tatgatgctg cgggaatgag gcagctcttt
      601 ctggcaggac cgtttctcat agcgaatctc ttcgggctga tcatgggcgt cttccttatg
      661 ataagcactt ttttcttcgg ggatgacatg agcgtcctga tacccctgcc cttaaagtca
      721 acagaggttc tgatgtcgaa gttcctggtt gtgcttatgg atctcatggt aatttcgatt
      781 gcggtcattc ttcctgccta cattctctac gggatcagat ctggagccgg tatttcatac
      841 tggatattca tggcgataat ctttctactc agtcaggtgt tgcccataat gctctcggcg
      901 ataatcgttc tgccgctttc gagactcttc cgcttcaaga gatacagaga cagcatcgtc
      961 tatctgatca gcgcgcttgt cattataggc tcgcttgttt ttgtttccct taccagcagg
     1021 gttgacccga acatgagcgc agaggagttc gcgagaatat tctcgagcga agatgcaata
     1081 ctaaacaaga cggccggggc atatccgccg actatactcg ttacaaaggc cctgagcaaa
     1141 cccttcttag agggtttact gtggttgctt gccttcattg gactgcacgc tgccgctctc
     1201 ggaatctttc tcttctttgg tcacaaattt tactactcgg tctattcgag cctccaagag
     1261 aattttgcca aacggtcgaa acttaaagaa ggggagatag caggtctgat gggcttacaa
     1321 cagacaaagt tctctgctct ctataaaaga gaatggtggt acttcctgaa ggtccctgca
     1381 ttcgccttca acggattcgg aaatgtactg gtcttcccaa ttctgctggt catagcggcc
     1441 gttgctggcc aaaccgatca gttaggtcag atgctggatc agtttcagca gtacaaacca
     1501 ctcttcgttc cggtcggcgc actggtagca gccgtcgcag gaggaataaa tggacttgcc
     1561 tcctccatgt tcagcagaga aggagatttg atcagggaac tgaaggttct tcccgttgac
     1621 gtaaatgaaa tcgtgaaagt caaatttctt catatcgaga cgcttagttt gataggcccg
     1681 gcattcggcg cagtcgctct tagtctgatc cttggtctgt ctttcttaga agcgcttgtt
     1741 gtatttgttg ttggagcatt gactctcaca ttcttgaaca ttctacaaat gataatcgac
     1801 tcgataaagc cgatccttga atgggagaac cctcagagag ccatgaagca gaacgttaac
     1861 gtcgcgcttt cgattccggt cgtctttggt tacgtgggcg gtcttggata tctcgcgttc
     1921 ttacttaagg acaacatatc cgggacaatt atgactatca tattgaccgc gatcgcttta
     1981 gttggtattg tattcacgtg gcctatactt atcaagaaag cgaacagact ctttgctaga
     2041 gatttgtgaa aaaccgtggt cggtggtagc aggtaggggg ttcgtaagag caagagaaac
     2101 tggttgattg ttgagcggtt ctccgttctc ccgaacgaaa gattatgagt gactagaaag
     2161 aaacgtcgag attctgaaac aagttcagaa tgactgtatg tgacgttttc cgagaaccct
     2221 ccgggatgct gaaacaagtt catgacacga tgtgacgttt tttgcgaaag gcgtttagaa
     2281 gttgaaacaa gttgagaata acgtgatact gaacaaagaa ccaagaacct ggacgcgtag
     2341 cgtcggaacg aagaacaggt cttctcgaag gacgggtcca cgatcttgga cgaaggacga
     2401 gggacgtttc tttactttag gtcttgcaac tgagaacttg taactgatct tttggcgcac
     2461 agcggctttt tcgaaggacg tgtcattgat tttggacgaa ggccaagaac ggcttctttc
     2521 agcatgattg caagtgccgt tctcgcaatt acctaacacc gtcatcccga acttgtttcg
     2581 ggatctagac ttaaatactc gaacttaaat tagac
//
LOCUS       DAOQYI010000121         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_44728_length_2558_cov_82.018777, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000121 DAOQYI010000000
VERSION     DAOQYI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44728_length_2558_cov_82.018777"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..1538)
                     /locus_tag="PKL93_11300"
     CDS             complement(324..1538)
                     /locus_tag="PKL93_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNQ71886.1"
                     /translation="MKFDREKIKSLIEKNGFENVGDVQEAMKELFGDVIKEMLEGELE
                     DELGYSKHDYKNKETDNSRNGHSKKTVRSDYGEIELEIPRDRKGEFEPVVVKKHQRDI
                     SGIEDQIISMYARGMTVRDIQSHIEDIYGSRLSAESVSRITDKILPLVSEWRNRPLES
                     AYAIVYMDAVFFRVVESNQVRKKALYIALGVSLNGHKDILGMWISETEGASYWLSILN
                     ELKNRGVEDVAIFSIDALSGMEEAIEAVYPFSEVQKCIVHQIRNTMRYVTWRDRRPLA
                     RDLKTIYQAPTREAGWNALLALEEKWGDKYHLSIKSWKDNWETLSTFFQYPEELRRLV
                     YTTNPIESVNRQLRKVTKNKGVFPTDNSLLKMAYLAIMNITKKWTVRTLDWSRILTQL
                     VIKYERLAKYIS"
     gene            complement(1807..2235)
                     /locus_tag="PKL93_11305"
     CDS             complement(1807..2235)
                     /locus_tag="PKL93_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71887.1"
                     /translation="MRRKASVDAMPTPSGSDAPKSIGGSDAAAQEVMPGVSSGEMMLV
                     RKRQEEIEKTKSSDAGQQGSAWQVSPSARPVPLRGRRAAARLKVENPLSAVKSHPKKE
                     QFFVPTLRIQVLRYWSSTSRYLNLFQHLAPKSRWTLAERR"
BASE COUNT          605 a          588 c          534 g          831 t
ORIGIN      
        1 agttcaagat tgtcagaaga atatgaaccg tggttggggg taggaggtta ggggtcggaa
       61 agagcgaagg atcagtttca agttccaagt tgtgagttgt aaaaaaggcc ggggtcggag
      121 gttggtggtt ggaggttcgt aagagcaaaa gaaactggtt gaccgctgag cggttctccg
      181 ttctccgaga agaacggttg tgagttccta gatgcaagtt gcaagaaaaa gaaaggagac
      241 ctgttttaat ggcaatgaag taagctcgtt acggttctgt ctcttttttt gtgtaaaccc
      301 cttttttgta atgggtttaa gaatcagctt atgtacttag ctaatctttc atactttatc
      361 acaagttgag taaggatcct ggaccaatca agagttctta ctgtccattt ctttgttatg
      421 ttcattattg ctagataagc catctttaag agagaattgt ctgttggaaa gactcctttg
      481 ttcttcgtta ccttcctgag ttgtctatta actgattcaa tgggattagt tgtataaacc
      541 aatcttctca gttcctctgg atattgaaag aaggttgaga gagtttccca gttatctttc
      601 cagcttttta ttgacaagtg gtatttgtcg ccccacttct cttctagtgc aagcaatgca
      661 ttccatcctg cctctcttgt aggtgcctga taaattgtct tgagatcgcg tgctagaggt
      721 ctacgatccc tccaggtaac atatctcatg gtgtttctta tctggtgaac tatgcacttc
      781 tgaacctctg agaatggata tacagcctct atggcttctt ccattccgct taatgcatct
      841 attgagaaga ttgccacgtc ttcaaccccg cgattcttca attcgttgag aatgcttagc
      901 cagtagcttg ctccttctgt ttcacttatc cacatgccta gaatgtcttt gtggccattt
      961 agggatactc ctaaggctat atagagtgct ttcttcctta cctggttgct ttcaacgacc
     1021 ctgaagaata ccgcatccat gtagactatt gcgtacgcag attctaatgg tctgtttctc
     1081 cattcggaga ccagtggcag tatcttgtcc gttatcctgc ttacgctctc tgcagacaat
     1141 ctggaaccgt agatatcctc tatatgactc tgaatatctc ttactgtcat tccccttgca
     1201 tacatggaga ttatctgatc ctctatccca gaaatgtccc tctggtgttt cttcacaaca
     1261 accggttcaa attcaccttt tctgtctctt ggtatctcaa gttctatttc tccgtaatcg
     1321 cttctaactg tcttcttgct atgtccattc ctggaattgt cagtctcttt gttcttgtag
     1381 tcatgcttcg aatatccaag ctcatcttcc agttctcctt caagcatttc tttgattacg
     1441 tcaccaaaca attccttcat tgcctcttga acatctccga cgttttcaaa tccattcttc
     1501 tcgattaacg acttgatctt ttctctgtca aatttcatct tcttttccct ccattcttct
     1561 cttccattct agagggttta cacaatctta tttacatact cctcgttact gacattcaac
     1621 aagagaacat catcgagatt ctgaatcaag ttcagacgga ttctttcgct cagcgaccag
     1681 cgggtttttt ctcttaagcg cacagcggtt cttatgctcg caactggaac agtcaacttg
     1741 caactgctct tcgaaggacg ggttcttgct ctttaacgat agaccaaaaa cggctcctca
     1801 cagcgctcag cgtctctcgg caagtgtcca gcggctctta ggagcgagat gctgaaacaa
     1861 gttcagataa cgggatgtgc ttgaccagta acgaagaacc tggatgcgga gcgtcggaac
     1921 gaagaactgc tcttttttgg gatggctctt tacagcgctc agtggattct ccacttttaa
     1981 gcgtgcagcg gctcttctcc cgcgaagcgg aactggcctc gccgaaggcg atacttgcca
     2041 ggcggagcct tgttggccag cgtcagaaga cttggtcttt tcgatttcct cctggcgttt
     2101 acgcaccagc atcatttccc cggatgatac tccgggcatc acttcctgcg cagcagcatc
     2161 acttccgccg atgctttttg gcgcatcact tcccgacgga gtgggcattg catcgaccga
     2221 tgcttttcgg cgcatgctct tctctttcgg aggtggacca tcaacgggca acgaccatta
     2281 gcctcgtatt atcgaggtga agaattcgaa atctctttct tattgctttc tgaactaatc
     2341 ttaccagcca gtgtgtcaaa gaaaatgatt acgacggaaa gattagccct agttcaatct
     2401 aatgaatggc aacagcttta atatgatgat gcttaggtgt tacataattt atgttataac
     2461 taagtatcgc aaaaccgagt aggttactct attttggaga gaataaccat ttaaggtatt
     2521 attgtataga tgttttttca ctattgaaag tgggttat
//
LOCUS       DAOQYI010000122         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_47990_length_2446_cov_83.311585, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000122 DAOQYI010000000
VERSION     DAOQYI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47990_length_2446_cov_83.311585"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(417..2237)
                     /locus_tag="PKL93_11310"
     CDS             complement(417..2237)
                     /locus_tag="PKL93_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HNQ71888.1"
                     /translation="MKILKNGNLMAVTDERGIIDCESEKAAGLYLEDTRFISRMILKA
                     SIQLKRLHVDFSWDRIEARYLGRSRPDVSNFDIFLSETLRVEGNTLYAELTVRNYSLE
                     EVQLTFDYEISCAFDDIFTIRGENDAYSGLETSRTVSASSLNSLEYESDYEKDVIENS
                     LPSLSLKLRPGQSATASGKLRLSKAVKKEEIFKDMLSERPVGNLQLTRNVNQIGEREI
                     GDLKMLMIPTVYGDFPGAGLPWYATVFGRDSLIFGLQTVDLLPEITRNILKIHSLLQS
                     CEEEIYSEAQPGKIVHETRLNELSLSGRLPFERYYGTIDATLLFIMLSHRYYRQTGDL
                     DFIKSIEKNIMAAAKWIDEYADPDNDGYVEFAPSGGGLVNQGWKDSADSVNFADGRLA
                     EPPLALVEVQGYLYDAFKCLEKLVVLFDKQEMASLYASKAATLKPNFNRDFWLENESY
                     FATALDKNKTPVDSITSNPGHCLMTGIVDEDKADALADRLFSEDMYTGWGIRTLSSKM
                     KRYNPFSYHNGSVWPHDNSLIMLGLTNYGFSEKAKRLASDLLRAKEKYRDNRLPELFS
                     GLSILETSGKLIEYPASCSPQLWSIGTLFVISSVLAPDSA"
BASE COUNT          626 a          575 c          578 g          667 t
ORIGIN      
        1 aagtggaagg cgcccgtcga tgtctggata agagaaatgt acgataaggt aaaagaaaag
       61 agtgcaaaga aagcaaaagc taaggcaaag cgattgcgga aagaaagtga gaagttggcc
      121 gacagttcgt ctcgattgga ttacagcgat aaatcagaca agcaagacta agggtcgtca
      181 ttatagagat acacagccta atggcagtca aaggacacgg gtcccgattg gttattttgt
      241 atctaagaga cgaggaccag gaagcgtaga tcgaataacg ggactttcta tagctccgag
      301 atgaaagatc ttccgagcaa aagcctctac agctagtgga aactatcata gttctcttaa
      361 gttagttcga tagccagaat ggtttttcag gtctttcgaa tgtctagaga ttcatttcag
      421 gccgaatcag gagccagaac acttgagatc acaaagagcg ttccaattga ccagagctga
      481 ggggaacaac ttgcgggata ctctatcagc tttccggatg tctccaatat tgacaggccg
      541 ctgaagagtt cggggaggcg attgtcacga tacttttcct tcgctctcaa gagatctgag
      601 gcgagcctct ttgccttttc ggaaaagccg taattggtaa gcccaagcat aatcagcgag
      661 ttgtcgtgcg gccagacgct tccgttatgg tacgagaagg gattatagcg cttcatcttg
      721 ctcgagagag ttcttattcc ccagcctgtg tacatatcct ctgagaagag tctgtctgcc
      781 agagcatctg ctttgtcttc gtccactatt ccagtcatga gacagtggcc ggggttagag
      841 gttatcgaat ctaccggagt tttgttcttg tcgagagccg tagcaaagta gctctcattt
      901 tcgagccaga aatcacggtt gaaattgggc ttcagagttg ccgctttcga agcataaaga
      961 gaggccatct cttgtttgtc aaagagaaca acgagcttct cgaggcattt gaaagcatcg
     1021 tacagataac cctgaacctc tacgagggca aggggcggtt cggcaagcct gccgtctgca
     1081 aagttcaccg aatcggcaga atccttccag ccctgattca cgagtccgcc gccagaagga
     1141 gcaaattcga catatccgtc gttatcgggg tcggcatatt cgtctatcca ctttgcggct
     1201 gccatgatgt ttttctcgat ggatttaatg aagtcaagat cacctgtctg ccggtagtac
     1261 ctgtgagata gcattatgaa gagaagggtc gcatcgatcg ttccatagta tctttcgaaa
     1321 ggcaatcttc ctgacagaga aagctcgttc aggcgggtct cgtggacaat ctttcccggc
     1381 tgagcctcgg aataaatctc ttcttcacaa ctctggagaa gagaatggat cttcagtatg
     1441 ttcctcgtga tctctggaag aagatcgacc gtctggagtc cgaagattag actgtctctt
     1501 ccgaagacgg tggcgtacca tggaagtccc gcgcccggaa aatcgccata gaccgtcgga
     1561 atcataagca tcttcagatc tccaatttcc ctttcgccta tttgattaac gttcctagtc
     1621 aactggagat tccccacagg tctctctgaa agcatatcct tgaagatctc ttctttcttc
     1681 acggccttgc tcaatctcag tttgcccgag gccgtcgcgc tttgccccgg cctgagcttt
     1741 agagagagcg agggtagcga gttttctatg acatcctttt cgtagtcgct ttcatactcg
     1801 aggctgttga gtgaagaagc agataccgtt ctggacgttt ccagcccact gtaagcatcg
     1861 ttctctcccc ttatcgtgaa gatatcatcg aaggcgcagc tgatttcgta atcgaaagtc
     1921 agctgaacct cttcaagcga atagtttctt accgtcagtt cggcatacag ggtattgcct
     1981 tctaccctga gggtttcaga caggaagatg tcgaagttag aaacgtcggg tcttgatctc
     2041 cccagatagc gggcttctat tcggtcccat gaaaaatcta catggagtct ctttaactga
     2101 atcgaggctt taagaatcat tctggatatg aagcgggtat cttccagata aaggcctgcc
     2161 gccttttcgc tctcacagtc tataatccct ctttcatcgg taactgccat gagattcccg
     2221 ttcttcaaga tcttcacgag ttcacctctc ttgccgatcg agctcgtaga ttccgaaatc
     2281 tttttttgca tcaaaattgg ctgctttcag gactttactt tcaataattt cgactagccg
     2341 ccgtattttc gattcgaaat caaataaaac aactacgata agtatttcta ctggttactt
     2401 ctcttcggtc ttctcaatct ctgactgttt aagcattaac gcctct
//
LOCUS       DAOQYI010000123         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_49048_length_2412_cov_70.497242, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000123 DAOQYI010000000
VERSION     DAOQYI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49048_length_2412_cov_70.497242"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="PKL93_11315"
     CDS             <1..90
                     /locus_tag="PKL93_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ71889.1"
                     /translation="FDFSKAVPYVSSRLNLSRYTIYKYIKESS"
     gene            116..586
                     /locus_tag="PKL93_11320"
     CDS             116..586
                     /locus_tag="PKL93_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNQ71890.1"
                     /translation="MMLIEKRIKELGIELPPSSPPGAMYIPVKRLGNALFVSGQVPMK
                     DGKPAYTGKVGKERSIEYAEEGARLCIVNMLAAVKAYLGDLDRVVNIVKLQAFVNSET
                     GFDRQHIVANAASELLYQISGEAGRHARTAFGTNQLPLDFTVEIEAVIEIREND"
     gene            588..1799
                     /locus_tag="PKL93_11325"
     CDS             588..1799
                     /locus_tag="PKL93_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ71891.1"
                     /translation="MDYDFDTLVNRENQGNMKCMLTPDIVKKMNLISYAGAEMDFKTA
                     PAIIDALVKRARNGLLGFTLADEKYLSSVQWWMKNMRNWEIERDWIVPTYGTIHSVAT
                     AIRAFTKEGDGVIVQPPVYNRYEQAVRRTNREIVSNPLIYGDGKYSMDFDDLERCMRV
                     EKNRLFILCNPHNPIAKVWQRSDLEKISSLAQRYNVLVFSDEIFGEITFNGNSAIPYS
                     TVAGKEGNSIVATSLGKVFNLTGVNSANIVIPDPSTRKRFRIQRDADHYGSIDPMVHA
                     AVCAGYGPDRADWVREMREYVWENVSIMKDFFTRNIPAVTMTKVEGSFVIWIDWRRLK
                     LDDEELHSFLLNEAYLDLDRGVNYGEGGKGFTRMNIAAPRRKIEESLGFLFEAAARRG
                     YAVSETLNRWR"
     gene            1811..>2412
                     /locus_tag="PKL93_11330"
     CDS             1811..>2412
                     /locus_tag="PKL93_11330"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HNQ71892.1"
                     /translation="MKKLIEDYFEQNRELMIRDIMRLVRIKSDKQEAKEGKPYGEGPA
                     EVLAVALEMASEMGFKTRNYDNYVGAIDFDDQKNRLDILAHLDVVPGGDGWSVTEPFN
                     PVVKDGRLYGRGTIDDKGPAVAALYAMKAVKDLNIPLKAGVRLILGTDEECGSSDIRY
                     YYGIEEEAPMTFSPDASFPVVNIEKGGLQGKFESRFEEST"
BASE COUNT          719 a          510 c          666 g          517 t
ORIGIN      
        1 ttcgactttt ctaaggcagt cccgtacgtt tcaagcaggc tcaatctatc tagatacacg
       61 atatacaaat acataaagga aagcagctga cataaataaa gaaaaagagg agaagatgat
      121 gcttatagag aagaggataa aggaactggg aatcgagctg ccaccgtctt caccgccagg
      181 agccatgtat attcccgtaa aaaggctcgg aaatgctctt ttcgtttctg gtcaggtacc
      241 gatgaaggac ggaaagcctg cttacacggg aaaagtggga aaggagagat caatcgaata
      301 cgccgaggaa ggggccagac tgtgtattgt caacatgctg gcagccgtaa aggcttacct
      361 cggtgatctc gacagggtag ttaacatcgt gaagctccag gcatttgtga acagcgaaac
      421 tggcttcgac aggcagcata tcgttgctaa tgcggcttcg gaactccttt atcaaatatc
      481 cggtgaagct ggaaggcacg cacgcactgc atttggaacg aaccagctgc cgttggattt
      541 cacggtcgag atcgaggcag taatcgaaat cagagagaac gattaatatg gactacgact
      601 tcgatactct ggtaaacagg gagaatcagg ggaacatgaa atgcatgctc actcccgata
      661 tagtcaagaa gatgaatctg atcagttatg ccggcgctga gatggatttc aagactgctc
      721 ctgcaataat cgacgctctt gttaagcggg ccaggaatgg ccttcttgga ttcacacttg
      781 ccgacgaaaa gtatctttcc agcgtacagt ggtggatgaa gaacatgcgt aactgggaga
      841 tagagagaga ctggattgtg cctacctatg gtactattca ctcggttgcg acggccatca
      901 gggcattcac gaaagaggga gacggtgtaa tcgtacagcc tcccgtatac aacagatacg
      961 aacaggctgt aagaaggaca aaccgagaga tagtgagcaa tcctcttatc tacggggacg
     1021 gaaagtactc aatggacttc gatgacctag agagatgtat gagggttgag aaaaaccggc
     1081 ttttcatact gtgcaatcct cataacccca ttgcaaaagt ctggcagcgt tcagatctgg
     1141 agaaaatctc ctcgcttgca cagagatata acgtccttgt attcagcgac gaaatattcg
     1201 gggaaataac cttcaacggc aattcggcta ttccgtactc cactgttgcc ggaaaggaag
     1261 gaaactcgat agttgcgacc tcacttggaa aggtcttcaa tctgactggc gtcaacagcg
     1321 caaatattgt tatacccgac ccctccacaa ggaagagatt caggatccag cgagacgcag
     1381 atcactacgg aagcatagat cccatggttc atgcggcagt atgcgcaggc tacggacccg
     1441 acagggccga ttgggttaga gagatgaggg agtacgtgtg ggagaatgtc tccataatga
     1501 aggacttctt caccagaaat attccggcag tcacaatgac caaagtagag ggttcattcg
     1561 taatctggat agactggcgg aggcttaaac tagatgatga ggagctgcac tcattcctgc
     1621 tgaacgaggc atatcttgat ctggatagag gcgtaaacta tggagaaggt ggaaagggtt
     1681 ttacgcgaat gaacatcgct gcacccagaa ggaagataga agagtcactg ggattccttt
     1741 tcgaagctgc cgcgagacgt gggtatgcag tatccgagac tttgaacagg tggaggtgaa
     1801 gatcattcct atgaagaaac tgattgaaga ctacttcgaa cagaataggg agctcatgat
     1861 aagagatatc atgagactgg taaggataaa gagcgacaag caagaggcga aagagggcaa
     1921 gccatatggc gaaggaccgg ccgaagtgct ggctgtagcc cttgagatgg cgtctgagat
     1981 gggattcaag accagaaact acgacaacta tgtaggagcg attgacttcg acgatcagaa
     2041 aaacaggctt gatattctgg ctcatctcga tgtggtaccg ggaggagacg gttggtcggt
     2101 cacagaaccc ttcaacccgg tagtcaagga cggaaggctc tacggcagag gaacgataga
     2161 cgacaagggc cccgccgtag cagctcttta cgccatgaag gctgtcaagg atctcaatat
     2221 tcctctgaag gccggcgtga ggttgatact gggaaccgat gaggaatgcg gatcgtccga
     2281 cataagatac tattacggga tcgaagagga agctccaatg accttctcgc cggacgccag
     2341 tttcccggtt gtgaacatag agaagggggg acttcagggc aagttcgaat cgagatttga
     2401 agagtcaact ga
//
LOCUS       DAOQYI010000124         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_51137_length_2348_cov_80.201483, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000124 DAOQYI010000000
VERSION     DAOQYI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51137_length_2348_cov_80.201483"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PKL93_11335"
     CDS             <1..985
                     /locus_tag="PKL93_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNQ71893.1"
                     /translation="KAHSLSEEVINVMTLMENVQVSKLFISSKKDLFQSYIWSQGYLY
                     VVARRGKMTKYSMKGFSGLKGAEILDEMDEYVEKVVENARMLLSAERINPGTYDVICS
                     PDVAGLIAHEAFGHGVEMDMFVKERAKAVEYLGKQVASELVTMHDGAAGVREVSSYAF
                     DDEGCEAKDTVIIDRGILLTGISDVISASVLGTEPTGNGKRQSFERKAYARMTNTYFS
                     PGKDDLEEMITSIDYGFLLEDYYSGMEDPKNWGIQCVIAYGREIVKGKLTGRIVSPVM
                     MTGYVPTLLKSISMVSKDFRLSGTGACGKGHKEFVKVSAGGPYIKAKARLG"
     gene            985..2259
                     /locus_tag="PKL93_11340"
     CDS             985..2259
                     /locus_tag="PKL93_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNQ71894.1"
                     /translation="MIDKISRQLKSVAISGWKIKEENVESEEYFFVKDKVDLGRSKKV
                     SKYEVTLYKDFEDKNEKYRGSSVFYISPGMEDAEISQAIEEAFFAAGFVKNPWYPLAP
                     ALSRKFCSEEYELSELASEAVGAVFSQSKPVNSWLNSVEIFVIKNRDRILSSEGVDVS
                     LSKYRTYIESIVSSSGREEVELYDQLLLSVPDHERISMRISRLLEMVRERANATATPA
                     VKGVPVILTGEPVKEALRYYLMQSNAHLKYDKISEAELGNSVQSEEAGDRITLEVVPE
                     MEGSYYSLPFDNDGLLMKKRKVIENGVLNSFWGDIRYSYYIGIEPTGAVMNFSVEEGS
                     LSVEEMRKEPHLEVTHFSAVDVDETTGDFGGEIRLGWHFDGSKRIPVTGGSVTGNLKS
                     LDSLYLSRETELNGDYYGPVSLMMEGMKISGE"
BASE COUNT          712 a          399 c          628 g          609 t
ORIGIN      
        1 taaggctcat tcattatctg aggaagtaat aaacgtaatg actttaatgg agaacgtaca
       61 ggtgagcaag ctcttcatat caagtaagaa agacctcttt caatcttaca tatggtcgca
      121 gggatacttg tatgttgttg cgagaagggg aaagatgaca aagtacagca tgaagggatt
      181 ttcaggtctc aagggtgcag agattctaga tgaaatggat gagtatgtgg agaaagttgt
      241 agaaaacgca agaatgctgc tctcagcgga acgaataaat cccggtacgt atgacgttat
      301 atgctcgccc gacgtagccg gactcattgc tcacgaagct tttggccatg gagtggaaat
      361 ggatatgttt gtgaaggaac gcgcaaaggc tgtcgagtat ttggggaaac aggttgcgtc
      421 tgagttagtt actatgcatg atggcgcggc cggtgtaaga gaagtgtctt cctacgcttt
      481 tgacgatgag ggatgcgaag caaaagatac tgtgataatt gataggggga ttcttttaac
      541 gggaatatct gatgtgatct cagcatccgt tcttggaact gagccaacgg gaaacggaaa
      601 gaggcagtcc tttgagagga aggcgtatgc tcgaatgacc aatacttact tctcgccagg
      661 gaaggatgat ctggaggaaa tgataacttc gatagattac ggatttcttc tcgaagatta
      721 ttatagtgga atggaagatc ccaagaactg gggtatccaa tgtgtcatag cttatggtag
      781 agaaatcgta aagggaaagc tgacaggaag aatcgtatca ccggttatga tgacgggata
      841 tgttccaact ctgcttaagt cgatttcaat ggtgtcaaag gatttcaggc tttctggcac
      901 cggagcctgt ggcaagggac acaaagagtt cgttaaagtc tctgctggtg gtccatacat
      961 aaaagcgaaa gcgaggttgg gataatgata gataagattt ctagacagct taagtcggtt
     1021 gcgataagtg gatggaagat caaggaagaa aacgtcgaaa gtgaggaata cttttttgtt
     1081 aaggacaagg ttgaccttgg aagatccaag aaggtaagta agtacgaggt taccctgtat
     1141 aaggatttcg aagataagaa cgagaaatac aggggtagtt ctgtattcta cattagcccg
     1201 gggatggaag atgctgaaat cagtcaagca attgaagagg cattcttcgc tgccgggttt
     1261 gtcaagaatc cctggtatcc attagcccca gccctttcga gaaaattttg ttcagaggag
     1321 tatgagttat ccgaactagc aagcgaggca gttggggcag tgttctctca atcgaaaccc
     1381 gtaaattcct ggttgaactc cgtcgagatt ttcgtcataa aaaacaggga cagaattctc
     1441 agttccgagg gagtagatgt ctcactttcg aaatacagaa cctatataga aagcatagta
     1501 agctcatccg gtagagaaga agtggagctt tatgatcaac ttctcctatc agttcctgat
     1561 cacgagcgga ttagtatgag aatttcaaga ctgcttgaaa tggtccgcga gagagctaat
     1621 gccacagcca ctcccgcagt taaaggagtt ccggtaatac tcacaggaga acctgttaaa
     1681 gaggcattga ggtattactt gatgcagtct aatgcccatc tcaagtacga caaaatttct
     1741 gaagccgagc ttggtaactc tgttcagtct gaggaagcag gggacagaat aacactagag
     1801 gttgtaccgg aaatggaggg ttcatactac agccttccat tcgataatga tggtcttctt
     1861 atgaagaaaa ggaaggtaat tgaaaacgga gtcctgaata gtttctgggg tgacataaga
     1921 tactcttatt acataggaat cgaacctact ggcgctgtta tgaacttctc tgtcgaagaa
     1981 ggaagtctca gtgttgaaga gatgagaaaa gaacctcatc tagaagtcac acatttttct
     2041 gcagtggatg ttgatgagac gacaggagat ttcggtggag aaatccgact gggatggcat
     2101 ttcgatggtt caaagagaat tcccgttact gggggttctg tgacaggaaa tttgaagagt
     2161 cttgattctc tttatctttc gagagagaca gagctaaatg gagactacta cgggcctgtt
     2221 tcgctgatga tggaaggaat gaagatctcg ggagaatagg gtttctggag gttcatatga
     2281 taatcgagac aataaagatg agacattcga ctagaaagtt tcttccatat ggactctctt
     2341 ctgaggag
//
LOCUS       DAOQYI010000125         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_51460_length_2338_cov_58.061323, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000125 DAOQYI010000000
VERSION     DAOQYI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51460_length_2338_cov_58.061323"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..1018
                     /locus_tag="PKL93_11345"
     CDS             422..1018
                     /locus_tag="PKL93_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNQ71895.1"
                     /translation="MNEFEKIKQIAAENNGFVETCDVVAEGIRKEKLRRLLELGQLEK
                     VSRGVYVLSERLIDEYKILQMRCKSCVFSHTTSLYLLGLSDRAPSKYHITVPQGSNVT
                     HLSKERSDLVFHYVKRSLFNLGLASVKSPFGNTLRSYDSERTICDLLKEKDRFDIQIV
                     STALRSYFRGTHDISKLSKYSRILGVEDLLHTYTEVLL"
     gene            1015..1875
                     /locus_tag="PKL93_11350"
     CDS             1015..1875
                     /locus_tag="PKL93_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNQ71896.1"
                     /translation="MINPEKLKNEIRKIAAEKDLKAGEILHMFMFERFIERVARSSYK
                     EFFILKGGLLIASILGIGQRTTIDMDTTVKNLSMKEDTLKRVVTEIIDTAVDDGVSFK
                     FERIVTIREEDEYNNFRVSLEARLGRIRIPMRIDMTTGDAITPGEIDYKYRSIFGEEV
                     IVIKAYTLETILAEKYETILRRSVLSSRPRDLYDVHTLFRLKKDQISTPTLKKAIENT
                     ARRRGSSSILAHYESVVYELIDSEYQKRLWEKYQNENSYAKGIQFNDAVETVKIIGDL
                     LKTSDRLGFH"
     gene            complement(1987..>2338)
                     /locus_tag="PKL93_11355"
     CDS             complement(1987..>2338)
                     /locus_tag="PKL93_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71897.1"
                     /translation="VPIFAYSVQRVFRSLANSGSFTRDPVQVHHGMTALPVILTMFLV
                     RIPVLPPAQRFYHSDLADRSVKFWTVPTIARRAVLGRDAKIGTDARIPSVPIFAYGIQ
                     LAAKNGSFTRDPVQ"
BASE COUNT          688 a          499 c          568 g          583 t
ORIGIN      
        1 tattagcggc gagaaaaaaa ggagtgacga ttctcctaat gtctgtcccg tttttcgcgg
       61 gagaaaggga tcctgaccag gaacgttgtc aggatgacag acaaggctgt catcccgtga
      121 tgcacctgca cgggatctca ttcttcttgt tgccttgctc ttcaagcgat tagtgtcttc
      181 tagcaagcgg ccagcggttc ttagccggtg gacccatgac ggttggcctg gcaggcttgc
      241 cacacagcag catcacttct tgaagggaaa agttcttttc cggcattgta tccgaaggtg
      301 atttacggcg catcatgtca aatggcatat agtagcctca cctggcgtca aataaaaccc
      361 ggttgtacta acgtgcgtta atatgatatt attaaagcac gttagtacag aaggtgaaac
      421 gatgaatgag tttgaaaaga tcaaacaaat cgctgccgaa aacaatggtt ttgttgaaac
      481 ctgtgatgtc gtagcagagg gaattagaaa ggaaaagcta agacggcttc ttgagttggg
      541 acagcttgag aaggtctcgc ggggggtgta cgtcttatca gaaagattaa tagatgagta
      601 caagatactc cagatgaggt gcaaatcctg tgtcttctct catacaacat ccctctatct
      661 actcggtctc tcagaccggg caccatccaa ataccacatt actgtacctc agggatcaaa
      721 cgtaacgcat ctttcgaaag agcgcagtga tctggtcttc cattacgtga aacggtcgct
      781 ttttaacctg ggtttggcca gtgttaaatc gccttttgga aacactttaa gatcttacga
      841 ttccgaaagg accatctgcg atctgctaaa agagaaggac cggtttgata ttcagatcgt
      901 ctcgactgcc ttgagatctt acttcagggg cacgcatgat atttcgaaac tatcgaaata
      961 ctctcgcatt ttgggagttg aagatctctt gcatacttat acggaggttc tgctgtgata
     1021 aacccggaaa agctgaaaaa cgagattcgt aaaatcgccg cggagaaaga cttgaaggcg
     1081 ggagagattc ttcacatgtt catgttcgaa agatttattg agcgagtggc taggtcctca
     1141 tacaaagagt tcttcatatt gaaaggtggg cttctaatcg catctattct aggtattggg
     1201 cagagaacga caatagatat ggatactaca gtcaagaacc tttcaatgaa agaagatacg
     1261 ctcaaaagag ttgtgaccga aataatcgac acagcggttg atgacggggt tagtttcaaa
     1321 tttgagcgaa tagtgacaat ccgcgaggaa gacgaataca acaacttccg tgtttccttg
     1381 gaagcaagat tgggtcgaat cagaattcca atgagaattg acatgacaac tggagatgct
     1441 ataactcccg gagaaattga ctacaagtat cgctcaatct tcggagaaga ggtaatcgtc
     1501 ataaaagcat atacattgga aacgatactc gccgagaaat acgaaactat cttgagaaga
     1561 agtgtcttga gttctcgtcc tagagacctg tacgacgttc acacgctttt cagactgaag
     1621 aaagaccaaa tctccacccc cacactaaag aaagcaattg aaaacaccgc gcgtagacga
     1681 ggatcatcga gtatactagc tcattacgaa agcgttgtct acgaactcat tgactctgag
     1741 taccagaaaa gactttggga aaagtatcaa aatgaaaaca gctatgccaa agggattcag
     1801 ttcaacgatg cggttgagac agtgaagata atcggcgacc ttctcaaaac ctctgataga
     1861 ctgggttttc attgagttgc agcggaacgt tctttcgatc ctgagttcag gattctgatc
     1921 acgagcatcg tcagaatgac agtatgtgac gacttctgaa taacctagac cgccatgaca
     1981 tagtgtctac tgcacgggat ctcgcgtaaa agatccgttt ttcgctgcaa gctgaatgcc
     2041 gtaagcgaaa atagggactg acgggatcct agcatctgtc cctattttcg cgtctcttcc
     2101 aaggacggct cttctcgcta ttgtcggaac agtccagaat ttgacggacc tgtcggccag
     2161 gtcggagtga taaaatctct gtgcaggagg aagaaccggg atcctgacca ggaacattgt
     2221 caggatgacg ggcagggctg tcatcccgtg atgcacctgc acgggatctc gtgtaaaaga
     2281 tccgctgttc gctaaagaac gaaaaacccg ctgaacgctg taagcgaaaa tagggact
//
LOCUS       DAOQYI010000126         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_52316_length_2314_cov_87.397078, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000126 DAOQYI010000000
VERSION     DAOQYI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52316_length_2314_cov_87.397078"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PKL93_11360"
     CDS             complement(<1..974)
                     /locus_tag="PKL93_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNQ71898.1"
                     /translation="MSDLLVIAERRGNEIHSSTFELLGKARELSIKRPLTVSVVVLSD
                     KPVEEESAYALFSGGADRIILAVDRSFSRFNFEPYTKTLASIVREFAPEIVLAPATTS
                     GRTYMPGLAALLKTGLTADCTGLDIEEETGNLLQTRPAIGGNIMATIKTPNHRPQMAT
                     VRPKTFSALKEAYESKSELVNFEVTPDLTQTEAKLIEFHPIGAGQKGVQDAEVIIAGG
                     MGLRKVENMEPIFRLSKMINGTVGASRKIVDSKWIGHEAQVGLSGHTVKPKIYIAAGI
                     SGAVQHIAGMQTAEFIVAINRDRNAAIFNFADIGLVGDAVEILNELAKS"
     gene            complement(967..1764)
                     /locus_tag="PKL93_11365"
     CDS             complement(967..1764)
                     /locus_tag="PKL93_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNQ71899.1"
                     /translation="MKILVLLKQVPDSDEVKLNPETGTMIREGVGTVINPLDLHALET
                     ALRIKETGDHSVTVVSMGPPAAEEAVREAIAMGADRAVLLTDRKFAGADTWATSMALA
                     RFAEKEGPFDLILAGEKATDGETGQVGPETGAMLGIPVATYVSRIAELDESGITIERE
                     VEDGKEIWKLPLPSLVSVTKDVNEPRLPTLNGKKLARNASVERIGNDFLELDPSEIGL
                     KGSPTRVVRIGTPKLSRKVEMYEGSSIRDGIDEIKKRLAPYLEVNHE"
     gene            2151..>2314
                     /locus_tag="PKL93_11370"
     CDS             2151..>2314
                     /locus_tag="PKL93_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNQ71900.1"
                     /translation="MKNGKAFLSFFLVLLSTFAFADKLTFGGDSAYPPYEFVDENGEY
                     TGFNVDLMKAV"
BASE COUNT          525 a          585 c          505 g          699 t
ORIGIN      
        1 gactttgcaa gttcgttcag aatctcgaca gcgtctccaa caagcccaat atctgcgaag
       61 ttgaaaatcg cagcgttcct gtctctgttt atcgcaacga tgaactctgc ggtttgcatc
      121 cccgcaatat gctgaacggc gccggaaata cccgcagcga tgtagatctt cggtttaact
      181 gtatggccgc tcaatccgac ctgagcttcg tgtcctatcc acttcgaatc aacgatcttc
      241 cttgaagcgc caacggttcc gttgatcatt tttgaaagcc tgaagatcgg ttccatgttt
      301 tcaacctttc gaagtcccat gcctccggcg attatcactt cggcgtcctg aactcccttc
      361 tgccccgctc ctattggatg aaactcgatt agttttgctt cagtttgggt caaatctgga
      421 gtcacttcaa agttaaccag ttcacttttg ctttcatacg cttccttcag agccgagaat
      481 gtcttgggcc tcacggtggc catctgaggc ctgtgattcg gggtctttat cgtcgccatt
      541 atgttgccgc ctatggcagg tcttgtctga agaagatttc cagtttcctc ttctatatct
      601 aggccggtgc aatcggccgt caggccagtc ttcaagagag ccgccaggcc gggcatatag
      661 gttctgcctg aagttgtggc cggtgcaagt acgatctctg gagcgaactc tcttactata
      721 gaagccagag tcttagtgta tggttcgaaa ttgaatctgg agaagcttct atccactgcc
      781 aggataattc gatctgcccc tccagagaag agagcgtaag ccgattcctc ttcgacaggc
      841 ttgtcggaaa gaaccactac agaaaccgta agaggccgct ttatggataa ctcccttgcc
      901 tttccaagta gttcgaatgt acttgagtgg atttcatttc ctcttctttc tgcgattacc
      961 aggagatcac tcatggttca cctccagata gggtgccaac ctctttttta tctcatcaat
     1021 tccgtctctg atactgcttc cctcatacat ctctaccttc ctagagagct tcggggttcc
     1081 tattcttact acccttgtgg gtgatccttt caagccgatc tcagatggat ctaattccag
     1141 aaaatcgttg ccgatcctct ctactgatgc gtttctcgcg agtttctttc cattcaaggt
     1201 agggagtctg ggttcattga catccttagt caccgaaaca agcgaaggta ggggtagctt
     1261 ccatatttcc tttccgtctt ccacttcgcg ctcgattgta atgcccgatt catctagctc
     1321 agctattcta gaaacatatg tagcaacggg aatacccagc atagcaccgg tttcggggcc
     1381 cacctgaccg gtttctccgt cggtagcctt ttcgccggcc aggattaggt cgaagggacc
     1441 ttccttttcc gcaaacctcg ctagagccat agacgttgcc cacgtatcgg cacctgcgaa
     1501 cttacggtca gtcagcagaa ccgccctgtc ggcacccatt gctattgctt ccctcacggc
     1561 ctcttctgcc gctggtggtc ccatagaaac aaccgtaacg gagtgatctc cagtctcttt
     1621 aattctcaat gccgtttcca gcgcgtgcag gtcgagagga ttgattacgg ttccaacacc
     1681 ctctcttatc attgttcccg tctccggatt gagcttcacc tcatcggagt ccgggacctg
     1741 ttttagaaga acgagtattt tcaatacaat tccccccagg cgtgtattct ctcttcaatc
     1801 ttaatacacg tgtcgattac tttcgcggcc ttagtctaga ttatggaccg aaagtccatg
     1861 aaagacttgt ttttagccac ttgattatta ttgactgtta tgctttgaaa accgatgaaa
     1921 aattaatcaa gtgatgtaat tgttagcaca aagtggaagt gcacaaatgt agaccgcaaa
     1981 aacttttagg tatttttcca taatattgct tttgagttaa tggaaagtac tttgtagtct
     2041 gcttgcgagc gataaatatt acccacaaat gattccactg aattccgggt ttttagaact
     2101 tcaattattc gtaatgattg tgctattata tctctaatcg gggtgaatcg atgaagaacg
     2161 ggaaggcttt cttatcattt ttcttggttc tactaagtac ttttgctttt gcggacaagc
     2221 tcactttcgg cggtgatagt gcctatcctc cgtatgagtt tgttgatgaa aacggtgagt
     2281 acacgggatt caatgtggac ttgatgaagg ccgt
//
LOCUS       DAOQYI010000127         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_52701_length_2302_cov_63.843347, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000127 DAOQYI010000000
VERSION     DAOQYI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52701_length_2302_cov_63.843347"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKL93_11375"
     CDS             <1..252
                     /locus_tag="PKL93_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ71901.1"
                     /translation="LDEATANVDTETEALIQRAMERISKEKTMITIAHRLSTIRNADR
                     IMVIHKGMLVESGTHEELLSKGGIYADLYRLQYELGDIA"
     gene            complement(249..1724)
                     /locus_tag="PKL93_11380"
     CDS             complement(249..1724)
                     /locus_tag="PKL93_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ71902.1"
                     /translation="MDDYLGYNKHEYRRTRKLAIIEGSFFNLAFLVTQGFIVTGLALE
                     YGASEMLIAIIGVLPTLAQLVQLAAPLVLKLFKDRKKAMLFSALLGRIPIAFIPVTLA
                     LGIRAQSMLLVLLGLIAFGNSLVGTFWASIMGDVIDPATTGKYYGRRNLILSLSTMLI
                     TPVYSLILDRYEGSLGFIIVTSMATAFAMITIFLLKMHYAPPVKTFGRGRMFKEVFSN
                     LKFRQYLKFAFVWNFAITISGPFYAYHQLVNLKVSYSYLSILSIVASLTSMLMYVLWG
                     KISDQIGHQSVAEFGILGATCLALMWVFVTPQTAVFLLPVDAIVTGVVWSAINLCLFT
                     MMMGMIRGYSVESYVAVQAFLNGIGALGGSLLGGFIASYLKGKSITIFGMEFFGIQVI
                     FLVGASFRFLSFLLLRRVQTPKIKTVTQVFFNTMSTVGRRMATRPYEFPVLQLRSRKK
                     REEAEPLKLDLPTSMIDLSDIEGIEPEETTDDSDHEERSDS"
BASE COUNT          659 a          512 c          579 g          552 t
ORIGIN      
        1 ctcgatgaag ccactgcaaa cgtcgacacg gaaacagagg cgctcattca gagggctatg
       61 gagaggatat caaaggaaaa aacaatgatc acgattgccc acagactttc gacgatcaga
      121 aatgccgaca gaatcatggt gattcacaag ggcatgcttg tagagtcggg aacacacgag
      181 gagctgcttt caaagggcgg aatatacgcg gatctttaca ggcttcagta tgagctaggt
      241 gatatcgcct aggagtcaga gcgttcttcg tgatcgctgt catccgttgt ctcttcaggt
      301 tctatccctt caatatcgct gaggtcaatc attgaagtgg gcaggtcgag tttcaaaggc
      361 tcggcttctt ctctcttctt tctagatctc agttgaagga ccgggaattc gtagggcctg
      421 gtggccattc ttctgcctac tgtagacatt gtgttgaaga agacctgagt aacggtcttg
      481 atcttaggtg tctgtacacg cctaagaaga aggaaagaca agaacctaaa cgatgcaccg
      541 accaaaaaga taacctggat accaaagaat tccattccaa aaatcgtaat gctcttgccc
      601 ttaagataag aggctatgaa tcctcccagc agggaaccac cgagagcacc tatgccgttg
      661 agaaaagcct gtactgcaac gtacgactca acggagtaac ctctaatcat tcccatcatc
      721 attgtgaaga gacagaggtt tattgcgctc cagacaactc cagttacgat ggcatctacc
      781 ggtaatagaa agaccgctgt ttgtggagtc acaaagaccc acatcaatgc tagacaagtc
      841 gccccgagaa ttccgaattc cgccaccgac tggtgaccaa tttgatcgga tatctttccc
      901 cataatacgt acatcagcat cgaagtgaga gaggcgacta tgctcaaaat gctcagatac
      961 gagtaactga ctttaagatt gaccagctga tggtatgcgt agaatgggcc cgaaatagtt
     1021 atcgcaaaat tccagacaaa ggcaaatttg aggtactgcc tgaactttag atttgaaaat
     1081 acctccttga acattcgtcc acggccaaag gtctttacgg gcggtgcgta gtgcattttc
     1141 aaaaggaata ttgttatcat cgcaaaggcc gtagccatag acgtcacaat aatgaatccg
     1201 agagagcctt cgtaacggtc gaggatcaat gaatacactg gtgtaatcag cattgtgctg
     1261 agtgagagaa tcaggtttct tctaccgtaa tactttcccg tcgttgccgg atcaatgacg
     1321 tcgcccatta tagatgccca gaacgtcccc acgagtgagt tgccaaaggc aattaagcca
     1381 agaagaacaa ggagcattga ctgagctctt atccctaggg caagggtaac tggaataaat
     1441 gcaataggaa ttctaccgag gagcgcgctg aaaagcatag ccttctttct gtctttgaac
     1501 agcttcaaga caagaggagc ggccagttga acaagctgcg ccagcgttgg cagaacgccg
     1561 atgatcgcta ttagcatctc cgacgcgccg tattcgagag caagtccggt gacgatgaag
     1621 ccctgagtta caagaaaagc gagattgaag aaggagcctt caataatcgc gagttttctt
     1681 gttcttctgt attcgtgttt gttgtagccc aggtagtcgt ccattattgc tcccgtcgaa
     1741 atcaaaatgc agtagattca tttgagagtt taacaccgaa agaatcgtcc gtgtcgcttt
     1801 atcgaaactg ttgcgtttca ttttttagaa gtgtccaaaa aacctcgaag caactgcgtt
     1861 caagaaatgg agctattacg aaaggaagag cagggtaacg attatagggt ctcgggtttg
     1921 gtgcgatgat gatctgctaa gcagaggaaa gcgaggttag taccaagagg tcaaaaatac
     1981 gaagagcgag attctgaacc aagttcagaa tgacgtgaag agcggggttc gattacaggg
     2041 tctggggtct cgtgaagaga accggcaatc agctcagggt tcggttctgt cactgagaca
     2101 gttattcagg tagcgccttc aggaggcccc acacaccgtc atcccgaact tgtttcggga
     2161 tctaggtgtt gatcttatcc aaggacgggt ccatgatctt ggacgggcga cgaaggacgg
     2221 gtcttgtcag cgtccagcgg ttcttagatc gagatgctga aacaagttca gcatgactga
     2281 atgcggcgtt tccgaaatga cc
//
LOCUS       DAOQYI010000128         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_53509_length_2280_cov_49.202697, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000128 DAOQYI010000000
VERSION     DAOQYI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53509_length_2280_cov_49.202697"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..1447)
                     /locus_tag="PKL93_11385"
     CDS             complement(392..1447)
                     /locus_tag="PKL93_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71903.1"
                     /translation="MRKWVLVLMLLTLLVIMVTAKEGVYAFMGMVGTEGQSNAYVLDV
                     TDPANPIVLFQSSEMDVPDVTIIGDYAYFGTTYVMDFYEYADLVVVDISDIKKPEKVG
                     YLRDDQGQFLRMVTRDNYAFTAGYISGLHILDLSEPSKPSIIKTMKFDDFNGDDGVRD
                     VLINGNYLYLSGATITPEMYYTGANRMGIYVADISDPENPEIVGFVYTGLYGVIQFRI
                     RENYLYCCSEGKGIDIFDISNPRAPAHVRTVYYTNAGPECIAFHGRYAYVADFMDKGI
                     LIFDATNPTDLRLLNLVATQSKMGFAVDILDGMLYVADGPSGLLIYSLENPEMPQFVG
                     QFSIEAGLAFTVKVIKR"
BASE COUNT          645 a          531 c          475 g          629 t
ORIGIN      
        1 cagagaagag ttctacagaa ttgtagaagc cgagcttgaa gaaggatagt ctctcgacat
       61 tcaaatatga cgaagctgga attgacagaa aaacaccgat tggttcagca agatctcgca
      121 gaaaaggagt cacaacccca aatgcgcctg gggagtgaaa ggcttcataa acagcttgat
      181 gcaatcattc tgaatccaag tgcaaacgga attcaataat cctcttcgcc aaagcataaa
      241 taggagtctg accttaatta agtcaatacc aaatgagaaa ttgacttaat tcttctgtga
      301 gcgatactga gcaaacctct ctgacaaaga aaaggcgcct gtcgaatgac agacgccagt
      361 tgcttgatac tgtattagtt ttcaatcggt gctaccgttt gataaccttc acggtgaatg
      421 ccaaccccgc ttcgatactg aactgaccaa caaactgcgg catttcggga ttctctagac
      481 tgtaaattag caggcccgac ggaccgtctg ctacatatag catcccgtca aggatatcca
      541 cggcaaaccc catctttgat tgtgttgcaa ccagattgag cagtcgtaga tctgtcggat
      601 ttgtggcgtc gaatatgagg attcctttgt ccataaagtc tgctacgtaa gcataccttc
      661 cgtggaaagc aatgcattcg ggaccggcgt tggtgtagta gacagttcta acatgagccg
      721 gcgctcgcgg attagagatg tcgaagatgt ctatgccctt tccttcagag cagcaataca
      781 ggtagttttc tcggattctg aactgaatca ctccataaag acctgtgtag acgaaaccaa
      841 cgatctcggg attctcagga tctgagatat ctgcaacata tatacccatt ctgttcgcac
      901 ctgtatagta catctctggg gttatcgtcg ctcctgaaag atagagataa ttaccgttga
      961 tcaatacgtc ccttacgccg tcatcgccat tgaagtcgtc gaacttcata gtcttgataa
     1021 tagaaggttt ggacggttcg gaaaggtcta ggatatggag tcccgaaatg tagcctgcag
     1081 taaaggcata gttatccctg gtaaccatac gcaagaactg accctggtcg tctctgaggt
     1141 atccgacttt ctccggtttc tttatgtctg agatgtcaac cactacaagg tcagcgtatt
     1201 catagaagtc cataacgtat gttgtcccaa aataggcgta atcaccgatt atagtgacat
     1261 ctggaacatc catttcactc gactgaaaga gaactatggg atttgcggga tcagtcacat
     1321 cgaggacata agcgttgctc tggccttcag tacctaccat acccataaat gcgtagactc
     1381 cttcctttgc ggtaaccata atcaccagaa gagtcagtag catcaaaacc aatacccact
     1441 ttctcattac aatccctcct ttgatcgatt cattttctcg ctcttgtcac tcccgacttc
     1501 tcctttctct taccttctct tcagcagact tcacacctcc tttcctgacg ctgataggca
     1561 agagcaaagg gttaagtgga gcaaaagctt gatatctttt ttcaaatttt gagacaattt
     1621 tcggcgctta ctgaaacgtc agagttgcct tccaccatta caataaacct gttttgaagg
     1681 atcaatgggt acatttgctc cgaagttgat ggtaacactt taagaagaac ataacggtta
     1741 tgaatgtctg taaacattga cagataagca atgtagaagc cgaaaaggaa agcgttactt
     1801 caaaattaaa gaatccccgg tttctcgggt tccgatctct tgtcgagagt tcttttaaga
     1861 gccaagagag atcactagag atttcgagag ttcgcttcgc tcaggagaga aaagaaccct
     1921 ctttttctcg tcctctctct tcatctcgtg ttttgacgct cttccgctct tgcgaacgaa
     1981 gaacaaagct cctagctccg gaacaaagag ccgatcttcg aaaaatggag tcccgacttt
     2041 tgtctgcaga tttaaagatt ggagactcta tcattagagg ctaaacagtt aatcccgggt
     2101 tctggatttt catctggccg aatactgatt tcccagttgc caaaacacag gagctgagtc
     2161 acaaatctcg ttgttcttct gctctttgtc caaccgaaga aaacgctctc attccatcaa
     2221 ttattgtctt gataaatctt tcgtcggcaa gatactctgc aagagatcta actctttgtg
//
LOCUS       DAOQYI010000129         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_53644_length_2276_cov_70.706889, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000129 DAOQYI010000000
VERSION     DAOQYI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53644_length_2276_cov_70.706889"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..1019
                     /locus_tag="PKL93_11390"
     CDS             285..1019
                     /locus_tag="PKL93_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71904.1"
                     /translation="MEKISRNQVNEWFKNNQFLMHYQLINELSTLLPNRKPGTYYATL
                     HRLKKEGLIRNVRRGLYEAGSKREFEPAVSKELSGLFKKLRKSFPYLENLCVWNSRWL
                     NEFTVQQALSSMVIIETERGTEESVFEYIKASYSRVFIDPTPKEINNYILGCKKCFVV
                     RSLVTEAPVTSFDNVAIPRLEKILVDLVCEKDLFVSFQGEELRNIYHRALKDYDVSLS
                     TLRRYARRRGKLEEIDRLIVTQGEGM"
     gene            1016..1471
                     /locus_tag="PKL93_11395"
     CDS             1016..1471
                     /locus_tag="PKL93_11395"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNQ71905.1"
                     /translation="MITRNSATKEWLDTVMKSYKKNDPGIVEKTIMALTLLEELSVEG
                     LDFIFKGGTSLMLLLDSYNRFSLDIDILLPDKPKNLEDIFDRICNNGIFKSWFEDERK
                     TPSDVPKAHYGFVRESALDGRERAVFLDILYQEVGTRSLLKVPVRHGVI"
     gene            1487..2065
                     /locus_tag="PKL93_11400"
     CDS             1487..2065
                     /locus_tag="PKL93_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71906.1"
                     /translation="MLVTIQTLDALLGDKLTAFAPNTTGIPYGTGKEAEIIKQLFDLG
                     VLFDNLADLDEVRKSFVQNCMFELGYRKLDLSEVDVLNDCFDTAITLAYRGVYKREQF
                     SYLMTGIKAFKAFSFNSSYSLEDAIRSSAKVAYLVQLLKAGKGRHEKFRETIDLRDVN
                     ITNPSWSKLNKLKKTDPEAFFYLFNALKLIEQ"
BASE COUNT          674 a          482 c          556 g          564 t
ORIGIN      
        1 acaaatactc gaaacatcca tcgacagtct tgttgacaac ttcataagac agccgtgtta
       61 aacgaggtca acatcaatag tttttttcat tctctttcag tcttcacgtc gacgactgca
      121 aaatccctag aggctccatc aataggtaat tatgcttgat acccatctag aacaacaaag
      181 aactccccca ggttgaagga tcgtatttca gcaagaagca tatgctataa ttacaaataa
      241 cggcaattat ctatatttat tgccattatt tgtaggtgat ttcaatggag aagattagtc
      301 gaaaccaagt aaatgagtgg ttcaaaaaca atcagtttct tatgcactat caactgatca
      361 atgaactgag tacactttta cctaatagga agccaggtac ttattatgca accctgcacc
      421 ggctgaagaa agaaggactc atcagaaacg tcagaagagg cctctatgaa gccggaagca
      481 aacgtgagtt cgaacctgcc gtatcgaagg aactctccgg tttgttcaag aagcttcgca
      541 agagcttccc gtacctggag aacctgtgtg tctggaacag cagatggctg aacgaattca
      601 ctgttcagca ggcgctttct tctatggtga tcatagaaac tgagcgaggc acagaggaga
      661 gcgtgtttga atatatcaaa gcatcctact cccgggtgtt catcgatccg actccgaagg
      721 agataaacaa ctacattctc ggatgcaaga agtgttttgt tgtaaggtcg cttgtaactg
      781 aagcccctgt aacgtccttt gataatgtcg ccataccgag gttagagaaa atacttgtcg
      841 atcttgtctg cgagaaggat ctcttcgtat catttcaggg agaggagtta cgcaacatat
      901 atcacagggc tttgaaggat tacgacgtga gtctgagcac gctgagaaga tatgccaggc
      961 gacgcgggaa gttagaagag atagacaggc tcatagtgac ccagggagag ggcatgtgat
     1021 cacaagaaac tctgctacca aagaatggct ggatacagtc atgaaatctt acaagaaaaa
     1081 tgaccccgga atagtagaga agaccataat ggccttgacg ttgctcgaag agctttccgt
     1141 tgaaggcctc gacttcatat tcaaaggcgg gacctctctt atgcttcttc tagatagtta
     1201 caacaggttc tcactggaca tagacatatt gcttcccgat aaacccaaga accttgaaga
     1261 tatattcgac aggatctgca acaatgggat attcaaaagc tggtttgagg atgaacgtaa
     1321 gactccctcg gatgttccca aagctcacta tggattcgta agggaatcgg cgctggacgg
     1381 aagagagcgg gcagtctttc tggatatcct ttaccaggag gtcggcacaa ggtctctgtt
     1441 gaaagtccct gtgaggcatg gagtaatata gacagccccg ccagacgtgc tggtaaccat
     1501 acagactctc gacgctctgc tcggagacaa gctgactgcc tttgcgccga acacgacggg
     1561 tattccgtac ggcaccggaa aggaagcaga gataatcaag cagctcttcg atctgggagt
     1621 ccttttcgac aatctggccg atcttgatga agtgcggaag tcgtttgtac agaactgcat
     1681 gttcgaactg ggttacagga agctggactt gagcgaggtc gatgtcctga atgactgttt
     1741 tgacacggct atcacgctgg cctatagagg agtctacaag agggagcagt tcagctatct
     1801 tatgacgggt atcaaggcgt tcaaagcatt ctcgttcaac agtagctact ctctggaaga
     1861 cgccataaga agttcggcaa aggtagcgta tcttgtgcag ttgctgaagg ccggtaaggg
     1921 acgtcatgag aagtttcgag aaacaatcga cttacgtgat gtcaatatca ccaatccatc
     1981 gtggagcaag ctgaataagc tgaagaagac agatccagaa gccttctttt atctattcaa
     2041 cgcattgaag ttgatcgagc agtaggttcc aattacctgc gagtgaaatc aggtgcttgc
     2101 gtagagaatc tactttcgag ccggtcgatc ggtccgcgtg tctacatata tccggagaaa
     2161 tgatttacta tgtgtataga gtgtataagg cggtgatcgc atgaaagata ttcaggaaac
     2221 caaagagaga cacaatatca tgatgaaacc ttcttcatgg aaggttctga aggagc
//
LOCUS       DAOQYI010000130         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_55331_length_2232_cov_82.506201, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000130 DAOQYI010000000
VERSION     DAOQYI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55331_length_2232_cov_82.506201"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1193
                     /locus_tag="PKL93_11405"
     CDS             297..1193
                     /locus_tag="PKL93_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNQ71907.1"
                     /translation="MLKKSGDGMAILAYALVSFFWGSTYLAIKIGVEGMPPMFFAGVR
                     FLIAGLIMIAFSRIRGYAFPASMREFSRLSLIGLFMLLGGNGLVVFAERWVDSGVASL
                     MMATIPIFAGVLEHFFIRTTRLTLKALFGLLLGFFGVYFLLIPAEHGISIDIPGILML
                     LSASFLWSIGTVLSKTFKGNSSIVSNIGIQMFAGGVGLMFVSAITGEFSRVTFSMNSV
                     LAIAYLVVFGSLVAYSSYIYLLQKWPATKAGTYAYINPLVAVSLGAIFLDEKINFLMI
                     LAMGAILLGVLIVQKSKIKVSE"
     gene            complement(1253..2218)
                     /locus_tag="PKL93_11410"
     CDS             complement(1253..2218)
                     /locus_tag="PKL93_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ71908.1"
                     /translation="MAETLIKIKNLKTYFPVKKSIFSKKRFVRAVDDVNMDIPTGSTF
                     AVVGESGSGKTTLARTVLRLIEPTSGEIEFEGKDILGLKGKDLLEVRRNMQIVFQDPY
                     NSLHPRKLIKNIIGEGMKIHFKITDIEIRDRIAAVLKEVGLIDDHMYRYAHEFSGGQR
                     QRIAFARAMVLKPKFIVLDEPTSALDVSVQAMVLKMMKEIKVQENLTYMFITHNLSLV
                     DYIADRVAVMYVGEVVEMGDKSTIFKHPSHPYTDLLMASNPVPDPKFVREKRLLKGEI
                     PSSIDPPSGCRFHNRCPFADERCEREAPELREIKPGHFVRCHYPL"
BASE COUNT          520 a          533 c          506 g          673 t
ORIGIN      
        1 ccaaccccca acctgctacc tccgaccact tacggatcct gaacaggagt gcttcaggat
       61 gacgaaatga gttggttctt atcagatggc ctttattagc cttcttcacg ccatagatgg
      121 agatgcttgc cagaatcacg cgaggcaatc aaagcaacaa aagactgtca tcccgtgatg
      181 ggcttcccat tacagtcctt tttttcggaa aagtttcgaa accgtattgt ttttgaatga
      241 atattgttgg ataattcgat cattactcca atttccattt ggaagataga ggtcgtgtgt
      301 tgaagaaaag tggagatgga atggcgatcc tcgcttatgc tctcgtcagt tttttctggg
      361 gatcgaccta tctggccata aagatcggtg ttgagggaat gccgccgatg ttctttgctg
      421 gcgttaggtt cctcatagct ggtttgatca tgattgcttt ctcgagaatc aggggatacg
      481 cctttccggc aagcatgaga gagttctcga gattgtcgct tatcggtttg tttatgctac
      541 ttggcgggaa cggactcgta gtcttcgcgg aacggtgggt agattctggt gtcgcctctc
      601 tcatgatggc aactatacct atctttgccg gcgtgctgga gcattttttc ataagaacga
      661 cacgcttgac gttgaaggct ctttttggcc ttcttctagg gttctttggt gtctactttc
      721 ttctaattcc ggccgaacac ggtatttcta tcgacatccc cgggatacta atgcttctct
      781 ctgcgagctt cctctggtct attggaacgg tactctcaaa aaccttcaag gggaacagtt
      841 cgatagtgtc aaatatcggg attcaaatgt ttgcaggcgg tgttgggctg atgttcgttt
      901 ctgcaataac cggtgaattc tcaagagtaa cgttctctat gaattcggtt ctggcaatcg
      961 cttatctcgt tgttttcggg tccttggttg cctacagcag ctacatttat cttcttcaga
     1021 agtggcctgc aacaaaggcg ggtacttacg cctatatcaa tcctctagtt gccgtctcat
     1081 tgggagccat cttcttggat gaaaagatca actttctcat gattctggcg atgggcgcga
     1141 ttcttcttgg ggtacttata gtccagaaat caaagatcaa ggtatcagaa tagcaagtat
     1201 taaaaaaggc atgcctctag ggacatgcct cacagtaagt agtcttaaga actcagaggg
     1261 gatagtggca ccttacaaaa tgaccgggtt ttatctccct caattcaggt gcttccctct
     1321 cgcaccgttc atcggcaaat ggacatctgt tatggaatct acagcccgat ggcggatcaa
     1381 tcgaactggg tatctcaccc ttcagtagcc gcttttccct tacgaacttc ggatccggga
     1441 cggggttgga agccattaac agatcagtgt atgggtgaga gggatgtttg aagatcgtac
     1501 tcttgtctcc catctcaact acttcaccga catacataac ggccactctg tcggctatat
     1561 aatccacaag agatagattg tgtgttatga acatatacgt aagattctct tggaccttta
     1621 tttccttcat cattttgaga accattgcct gaacggagac atccagcgcc gaggtcggct
     1681 cgtcaaggac gatgaacttc ggtttgagaa ccatagctct cgcaaatgct attctctggc
     1741 gttgcccacc agagaactca tgtgcatatc tatacatgtg atcgtcaatc aaccctactt
     1801 ctttcaaaac ggccgctatc ctgtctctta tttcgatgtc ggtgatcttg aaatgaatct
     1861 tcatcccttc cccaattatg ttcttaatca gttttctggg atgaagcgaa ttgtacgggt
     1921 cctggaagac gatctgcata tttcttctca cttcgagcag gtccttgccc ttgagtccaa
     1981 gtatgtcctt tccctcgaat tctatctcac ctgaggtcgg ctcgataagc ctcaaaacag
     2041 ttctggcaag agtcgtcttt cccgagcccg actctccgac cacggcaaag gttgacccag
     2101 ttggaatgtc catatttacg tcgtctacag ctctgacaaa tcgcttcttc gagaagatcg
     2161 acttcttcac cgggaagtaa gtcttcaggt tcttgatctt gatcaaggtc tcagccatct
     2221 tctctcacct cg
//
LOCUS       DAOQYI010000131         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_57208_length_2185_cov_68.200000, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000131 DAOQYI010000000
VERSION     DAOQYI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57208_length_2185_cov_68.200000"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..446)
                     /locus_tag="PKL93_11415"
     CDS             complement(18..446)
                     /locus_tag="PKL93_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNQ71909.1"
                     /translation="MLEDAVGKQYSRNDVERNCAEAMVYGANDEYGLDLPQEAFFTMG
                     AFGGGMRIKSICGAVTGSLAVLGILFNSEIYEKQERMKRIAEEFIAEFEKRYGHLECS
                     VLRDKYRDPVIGCETTVRMAARVLEEIIQKYRREITYRAK"
     gene            555..1718
                     /locus_tag="PKL93_11420"
     CDS             555..1718
                     /locus_tag="PKL93_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNQ71910.1"
                     /translation="MSILLKNGTIYPITSESFVGDILMNKGKIERIGQGLEEPGAEVI
                     DVSGKYVFPGFIDAHSHIGIYEEGVGGYYGDGNEATDPLTPDVSVVDAFNPQDTAIKR
                     ALSGGVTTVMVVPGSANPVGGQGAIFKFKKTLIVDDMVVRSPAGLKMATGENPKRVYG
                     EGFKKTPSTRLGTAAVIRGYFQKVRAYMVKKKAAEKEGKPFTDFDPKLEVGEKVLRKE
                     IPARIHAHRQDDILTAIRLSKEFDFDLVIEHATEGYKIVDFLKENNVPVVLGPIFGFR
                     TKLELKDMSYDAPRILNEKGVLAAFMCDHPVIPLENTNVQLGVALRYGSKEEDLLKMV
                     TINAAKILKIDERVGSLEVGKDADIAIWTKHPFNLAAKAERVFIEGEEVYSDL"
BASE COUNT          614 a          498 c          515 g          558 t
ORIGIN      
        1 aatgttttat ggaagactca cttcgctctg tacgtaatct ctctcctgta cttttgaata
       61 atctcttcga gaacccgagc cgccattcgt acagttgttt cacatccgat cacaggatca
      121 cggtacttgt ctcttagcac tgagcattcg agatgaccgt atctcttctc gaattcggca
      181 atgaactcct ctgctattcg tttcattctt tcttgttttt catagatctc gctgttgaag
      241 aggattccaa gtactgcaag tgaacctgtg acggctccgc agatactctt gattctcatg
      301 cctcctccga aggcgcccat cgtgaagaag gcctcctggg gcaggtcaag gccgtactca
      361 tcgttcgctc catataccat tgcctctgcg cagttcctct ccacatcgtt tctcgaatac
      421 tgttttccaa ccgcatcctc aagcatattt attcccttcc taaagccttg ttgaaatcaa
      481 aggcagtcat aagtttccga tacgtaccac ttctgataca attattgtat aactttcaat
      541 aaaggaggac ctgtatgtca atactactaa agaacgggac tatttacccc atcacttctg
      601 agtccttcgt aggtgatatt ttgatgaaca agggaaagat cgaacggata ggacaaggac
      661 tcgaagagcc tggggctgaa gttatcgatg tttcgggcaa gtacgtattt ccgggattca
      721 ttgatgccca ctcacacatt ggtatttacg aagagggggt tgggggctac tatggtgacg
      781 gaaacgaagc gacggatccc cttactccgg atgtctccgt tgtcgatgcg ttcaatcccc
      841 aggacacagc aataaaacgg gcattgtccg gaggtgttac tacagtcatg gtagttcccg
      901 gcagcgccaa tccagttgga ggacagggtg ctatcttcaa gttcaagaaa actctgatag
      961 ttgatgacat ggtcgttcgt tccccggcgg ggctgaaaat ggcaaccggc gagaacccaa
     1021 agagagtcta cggtgagggt ttcaagaaaa ctccttccac gagactggga acggcagctg
     1081 ttatccgtgg ctacttccag aaggttagag cctatatggt caagaagaag gcagccgaaa
     1141 aagagggaaa gcctttcacc gattttgacc cgaaactaga agtcggggaa aaggtactga
     1201 gaaaagagat ccccgctaga attcacgctc acagacagga cgatattctc acagcaatca
     1261 ggctctcaaa ggagtttgat ttcgatctag tgatcgaaca cgcgacggaa ggttataaga
     1321 ttgtcgattt tctcaaagaa aacaatgtgc cggtagttct tgggccaatc ttcggattca
     1381 ggacaaagct tgaattgaaa gacatgagct acgacgctcc aagaatactg aacgaaaagg
     1441 gagttcttgc agccttcatg tgcgatcacc ccgttattcc ccttgagaac acgaacgtgc
     1501 agcttggcgt agccttacgg tacgggtcaa aagaggaaga tcttttgaag atggtcacca
     1561 taaatgccgc taagatcctg aagatcgacg aaagagttgg atctctcgaa gttggaaaag
     1621 atgccgatat tgcaatctgg acaaagcatc cattcaatct ggcagccaag gcagagagag
     1681 ttttcataga aggcgaggaa gtctacagcg atctttgaag cacacacttc cagaagaaga
     1741 tcaaagaaaa attgtatgta atcaggatca gatagattcg aaacttcgct gtaccgcgct
     1801 tttttgccag aagtgatggg gtttcacacc aacggaaatt agatgttccg attcacactt
     1861 caatttggat ccgagagaga aaagtattat aatagtaatg cggtcacccg gaagggtttc
     1921 acgcactagg ccatagagcc tgctcacggc gaaaagccgt tgatgaagac gtgggagtat
     1981 tcccacgcct tctttatttc ttctctttag taatactcaa attatggagt caactgaaac
     2041 gaacgagatg atagagaagt tcagatgagc acatttttaa ggtttaggaa aagcaaaacg
     2101 ttcgcatcac gggtaatttg cccaaaagct tcttcagtca agttgcagaa gtcccacggg
     2161 tcctaagcct tccgctgaaa gctgc
//
LOCUS       DAOQYI010000132         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_58929_length_2145_cov_67.241148, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000132 DAOQYI010000000
VERSION     DAOQYI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58929_length_2145_cov_67.241148"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKL93_11425"
     CDS             complement(<1..615)
                     /locus_tag="PKL93_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ71911.1"
                     /translation="MLSKQEIKKAIGLRVTQRKFQERQLSPEQKKEVNDALRDIVPIN
                     SESVLHWYFSPEFPSGSGIVLAKMSDFSTPSLVKYGFEGEQIVLNLTLKGFSTSWARL
                     PNYLSMIVVGFAADGSGDIVERASRFLYREANRKPLKEVVYGKEDYIDEKMKDIIDAG
                     RMAPSSFNRQPWKFEVLGENEIAIHGWKKIPLIYEEVIAIDLGVV"
     gene            complement(755..1291)
                     /locus_tag="PKL93_11430"
     CDS             complement(755..1291)
                     /locus_tag="PKL93_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNQ71912.1"
                     /translation="MKSIVVYFSYDESTKALAEAMASSIDADLLRLVPTGEFTRTYYH
                     HVENPEQIETTPGYDPDKEHIWGSESVTMNNSPELEPYSFDPNMYDFIVLGSPVWALT
                     YAPPINTFLQENKIQGKKIALFCTHEGMIGGALENLKKALKGNEVVQESDFESPSSRL
                     EEYIETAKEWVREVKESL"
BASE COUNT          582 a          524 c          411 g          628 t
ORIGIN      
        1 aacaacaccg agatcgatcg ctataacctc ttcatagatc agaggaatct tcttccagcc
       61 gtgaattgct atctcgtttt ctcccaaaac ctcaaacttc cagggttgtc tgttgaacga
      121 tgagggtgcc attcttcccg catcgataat gtctttcatt ttttcgtcta tgtaatcttc
      181 cttgccatat acaacttctt tgaggggctt cctgttagcc tccctgtaaa gaaaacggga
      241 agctctctcg acgatgtctc cactaccatc cgcagcaaag ccaacaacaa tcatagatag
      301 ataatttggc agtcgtgccc agctagtcga gaatcccttc aaagtaaggt ttagaacgat
      361 ctgctcgcct tcgaatccat acttcacaag acttggtgta gaaaagtcac tcattttcgc
      421 aagcacaatt cccgatccgg aaggaaattc tggagagaag taccaatgca aaacactttc
      481 ggagttaatc ggaacaatat ctctcagagc gtcattgact tctttcttct gttcaggaga
      541 caactgcctt tcctgaaact ttctctgtgt taccctcaac ccgattgcct tcttgatctc
      601 ctgtttgctt aacattcaac cacctcttca ctcctattct atataatttg acaataaaac
      661 tcaagattaa ttggctcagt agactaaacg tctcaaaaag aaaacgccga gagcatgccc
      721 tcggcgtttc ggtaaatcaa atctatggtg gaaactagag actctcctta acctctctca
      781 cccattcctt cgcagtctcg atatactcct ctaatcttga agaaggagat tcgaagtcac
      841 tctcctgaac gacttcattc cccttcaaag cctttttcag attctcgagg gcgccaccta
      901 tcattccttc atgcgtacag aagagagcga tcttctttcc ctggattttg ttctcttgaa
      961 gaaatgtatt gatcggaggt gcatatgtaa gagcccacac cggactcccc aagactatga
     1021 aatcatacat gttcggatcg aagctatacg gctcaagttc gggactgtta ttcatagtca
     1081 ccgactcgct tccccagata tgctccttat ctgggtcata gccgggcgtg gtctctatct
     1141 gttcgggatt ctccacatgg tgataatagg ttcgagtaaa ttcccccgtg ggaaccagcc
     1201 tcagaaggtc agcatcgatt gaagaggcca tcgcctctgc aagtgctttt gtgctttcat
     1261 cgtatgagaa ataaacaaca atagacttca tactattacc tcccttcatt gctattatag
     1321 cttgacttag aagcgaaatg aacgtatctc tacaaaggaa ccgctattct ttgccagaac
     1381 aaatgaaaca gagaaggatt tctgaaaaac ctgcgccgat ttatcattca ggtgttggtc
     1441 tcccttgaat cagtgactcg gggaagttgg gttttgtcga ccgtttcgag aagttttagt
     1501 agaagcgcta aatcacaagt aatcaacagc aactttatgt tcttcagctc tcaaaggcac
     1561 ctttcaaaaa gtagagataa ctttccttat gcgcttcact ttgattgcat aatcgcttgc
     1621 cttgttagtt ctttcacctg tgtccacaaa aatcgatcga gcttgcgcga tacgaagtat
     1681 cctttagtaa tatcattatt tagtagaggc gggttttccg cttttggata atcggctcgc
     1741 ggctttcccg cttacggaaa atcggcttgc ggattaacgc ggcttcgaca gagcgtcttg
     1801 tcaatttctt atttgacttg ttccgatttg cttttttgcc gaaatttgag ccctctgaag
     1861 acatgaacaa agtaattcaa gttcccactg acttcagtca taaaaacgga gaacaaccca
     1921 cttaggcacg aacaacctga atgaaaacag ccgatgggag aaacctcatt ccgttttttg
     1981 ttacgcttag taaatcgaag atgcggagag catatgatgc tagaaagcga ctcaataagt
     2041 agttccaata agcctctcaa accattcacg gcttattact ctgtaatgtg gatgatagct
     2101 tctcttatac gaagcactgt gatgcaccag ttttccgcct tcaac
//
LOCUS       DAOQYI010000133         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_59509_length_2132_cov_51.918633, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000133 DAOQYI010000000
VERSION     DAOQYI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59509_length_2132_cov_51.918633"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKL93_11435"
     CDS             <1..204
                     /locus_tag="PKL93_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ71913.1"
                     /translation="EWCSDWYGDYSSVMQSNPYCNNGTSMVLRGGSWGLIDMEVRLAF
                     RWAGSRNGGVNDVGFRICRTSTD"
     gene            250..>2132
                     /locus_tag="PKL93_11440"
     CDS             250..>2132
                     /locus_tag="PKL93_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ71914.1"
                     /translation="MGTRRRTLLLVLFSLIVVTAKALPLFYGNYSLVSAAPKGKIVSD
                     RTYEDKIIRIEFDDFAGDYPEFTLRNISNTPLTIVWDLCAYVDNIGRSQRVFKTDMRY
                     LDIGKSIPPTLVIPGAYVEEAIVPVDSVEFDSEWIVHNSIPKEANQTISIVLTIEQQG
                     KQTTYTFKLTSEMLGGEVFVESGDFLMDDYFEDIWNDEEFKHRVIIAYDFYIGKYEVT
                     FTEYDQFCDVIGRTKPSDGGWGRGNRPVRAVTWWDAIAYCNWLSEKEGLPNAYDEKGN
                     LLDEYGNITSTVSNVVGYRLPTEAEWEYASKGGNKSRGYRYAGSDYLDEVAWFSDNSF
                     NEEASTNTTWPVGQKKPNELGIYDMVGNVWEWCSDSDGYYPSSVPTDPFRSSGDRRVV
                     RGGGYGLDELNIFLLNRLEGFPTDIYKDVGFRICRTGEYKGGNRPPVNPHKPEPSNAD
                     KCGKIPQILSWNCYDADYDVLSYDIYLDTKTDQMTKVASNHTDSIFSLGPLSYNTNYY
                     WRIVAKDSEGGITEGPIWSFSTHISLYKPSSEIQEMVLVEKGSFTMGDTWGDSWVIDR
                     PTHRVTLTYDFYIGKYETTFVEYDAFCEATGRSKPSDENWGRGDRPVINVSWWDAIAY
                     CNW"
BASE COUNT          660 a          368 c          533 g          571 t
ORIGIN      
        1 gaatggtgca gcgactggta tggagattac tcaagtgtaa tgcaaagtaa tccttactgc
       61 aacaatggta ccagtatggt tcttcgcggg ggaagttggg ggctaatcga tatggaggta
      121 cgactagcgt ttcgatgggc tggatcgcgc aatggtggtg tcaatgatgt cggattccgc
      181 atctgcagaa ctagcactga ttagtcccgt tttgaaataa tctcagctca accgagtggg
      241 ggtgaagaaa tgggtaccag aagaagaaca ctgttattgg tattattctc tttaatagta
      301 gtcactgcta aagctcttcc tcttttctat ggaaactaca gccttgtatc tgcagcacca
      361 aagggaaaga tcgtatctga taggacctat gaagacaaaa ttattagaat cgagttcgat
      421 gattttgctg gcgattaccc tgaattcact ttaagaaaca tatctaacac accccttaca
      481 atagtatggg atctgtgcgc ttatgtagat aacataggac gttctcaacg agtttttaag
      541 acagatatgc gctatttaga tattggaaaa tcaatcccgc caaccctggt aatacctgga
      601 gcctacgtag aggaagcaat tgttcctgta gattcagttg aatttgacag tgaatggatt
      661 gtccataatt cgataccaaa agaggcgaac caaacaatct caattgtact gacaatagaa
      721 cagcagggaa aacaaacaac ttatacattc aaactcacat cggaaatgct gggaggtgaa
      781 gtttttgtag aaagtggaga cttcttaatg gatgattatt tcgaagatat ttggaatgat
      841 gaagaattca aacacagagt cataatcgca tatgactttt atataggcaa atatgaggta
      901 acgtttactg aatatgatca gttttgcgat gttatcggta ggaccaaacc gagtgacggt
      961 ggatggggaa ggggaaatcg acctgttagg gcggttactt ggtgggatgc aattgcatac
     1021 tgcaattggt tgagtgaaaa ggaaggtctt ccgaatgcat atgatgaaaa aggaaacctt
     1081 cttgacgaat acgggaatat tacctccacg gtatctaatg tagttggtta taggctgcct
     1141 actgaggccg aatgggagta tgcatccaag ggaggaaaca aaagtcgggg ttacagatat
     1201 gctgggagcg actatttaga tgaagttgcg tggttttcag ataactcatt caacgaggaa
     1261 gcaagtacaa acacaacgtg gcccgtaggg caaaaaaagc caaacgagct gggtatttat
     1321 gatatggtgg ggaatgtttg ggaatggtgc tcagattctg atgggtacta tcccagttca
     1381 gtaccaactg atccttttag gagtagtgga gaccgacgag ttgttcgagg tggtggctat
     1441 ggtctggacg agctcaacat tttcttattg aatcgtttgg aaggttttcc tactgatatt
     1501 tataaagatg tgggatttcg tatttgtaga acaggggagt acaaaggggg taacagacca
     1561 cctgtgaatc cacataaacc agaaccatca aatgcagata agtgtggcaa aataccgcaa
     1621 atcttaagct ggaattgtta tgatgctgat tacgacgtat tgtcatatga catatatctt
     1681 gataccaaga ctgaccagat gacgaaagta gcttcaaatc atacagacag catatttagt
     1741 ctgggacccc tttcctacaa tactaactat tattggcgaa tagtagctaa ggattcagaa
     1801 ggaggaataa cagaaggtcc tatctggagc ttcagtacac atatttctct ttataaacct
     1861 tcaagtgaga ttcaggagat ggtacttgta gagaagggga gcttcacgat gggtgatacc
     1921 tggggagaca gctgggtaat agacaggccc acacacagag ttacgctaac ctacgatttc
     1981 tatataggaa agtatgagac aacttttgtc gagtacgatg ctttttgtga agcaacagga
     2041 agaagcaaac cctctgatga aaattgggga agaggcgaca ggccagttat caacgttagc
     2101 tggtgggacg ctatagccta ctgcaattgg tt
//
LOCUS       DAOQYI010000134         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_59562_length_2131_cov_69.829480, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000134 DAOQYI010000000
VERSION     DAOQYI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59562_length_2131_cov_69.829480"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..>2131
                     /locus_tag="PKL93_11445"
     CDS             48..>2131
                     /locus_tag="PKL93_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71915.1"
                     /translation="MDRETVKVTLLAEALEMSRNSYVEYRSLEGLESFFDRSTAIELA
                     KGLKDGFSLEIPLEVIMSNGFFRGYLAGLMLSDKTTLHVVKEQIAKILLDDLLRTMNE
                     AEETSPRQEESFQSERDLKKRFFSLFEEKEREESGGLVRENLSLFRHFMNSKHYIPIY
                     LWGNELTTFICESLDSEQFEGSLREDLRKIVPSVLASYSMPDTMGSSVGLRLPSIESY
                     LSSQFPILAMIRNRKLNLDQVTEFIKSLSHIIFGDILLAPVSMAAGGKTEEKARAKLI
                     INKMFASMSDSELGIMLNSSSYESRDGFLYEVVVCNDFSKLFDRLKNILKGIEKKASL
                     FSNMAIASALLDKDEAIDCVVEYVAYLKTLEPTIAVVRSGLLLCMKYDLEDEEFFRKK
                     FSEITDEKPDPPLIRSLLRDSFGDLDVIYENMADEYSHRTAIDGVISNLKSIKEPNFI
                     SFEKTGSLEYSRVPMYTLINSVISQNDIDHAFDTMVAYCKKYRFNFEFMNTLLFLSTR
                     ETDELKKLLHVEGELALNVLHDIVEKDKWASLIENEESPSNTELLQVIEHYVDSLIDR
                     KDYREALRVIEEVFPLVGPDDYGYLLASKLLVCWRLKMFDELAKTMEFNNHSGHPKAL
                     AVLALKNYAYRELDRAEQILLKILRHFPDFIKYLLEEEENDKKAFEKAERGNLRSQKR
                     VDSLTLAEEFRG"
BASE COUNT          620 a          409 c          530 g          572 t
ORIGIN      
        1 cgatgtatta atttatattg ttctactaca catgctagag gtggtatatg gatagagaga
       61 cagtaaaagt gaccctcctg gccgaggcac tggagatgag caggaattcc tatgttgaat
      121 atcgtagtct cgaagggctt gagtcattct ttgacagaag cacagcaatc gagcttgcga
      181 agggacttaa ggatggattc tctcttgaaa tacccctgga agtgataatg agcaacggat
      241 tcttcagggg ttatcttgcc ggtcttatgc tttcagacaa gactacttta catgttgtca
      301 aggagcagat agccaagatc cttctggatg atcttcttcg cacgatgaat gaggcagagg
      361 aaacttcacc ccgtcaagaa gagagttttc agtcagaaag agacttgaaa aagagattct
      421 tttccctatt cgaagagaaa gagcgcgaag aatctggagg acttgtgaga gagaatctct
      481 cacttttcag acatttcatg aatagtaagc attatattcc tatttacctt tggggtaatg
      541 aattgacgac tttcatttgc gaaagcttag attcggagca gttcgagggc tccttaaggg
      601 aagacttgag aaagattgtt ccttctgtgc tggcatcata ctcgatgccc gatactatgg
      661 gttcgtcagt aggacttcga ttaccgtcga ttgagagtta tttgagctcg cagttcccga
      721 ttctggctat gataaggaat cgaaaactga atcttgatca ggtgactgag ttcatcaaga
      781 gcctgtctca cattatattt ggggatatct tgttggcgcc ggttagcatg gcagcaggtg
      841 gaaagacgga agagaaagca agggcaaaac tgattatcaa taagatgttc gcttcgatga
      901 gtgattctga actcggtatt atgttgaatt cctccagcta cgagtcgaga gacggttttc
      961 tttacgaagt tgtcgtgtgc aatgatttca gcaaactttt cgatagactg aagaatattt
     1021 tgaaagggat agaaaagaag gcttccctgt tttccaatat ggctattgcg agcgctctcc
     1081 tggacaagga tgaggcaatt gattgtgtgg tagagtatgt cgcatatctg aagacgctgg
     1141 agccaacaat cgcagttgta cgatcgggac tcttgttatg catgaaatac gacttagaag
     1201 acgaggagtt cttcagaaag aagttttctg agataacaga tgagaaacca gatccgcctt
     1261 tgatccgatc tcttcttcgg gattcattcg gtgatcttga tgtgatctat gaaaatatgg
     1321 cagacgagta ttctcataga acggcgattg acggtgtaat ttcgaacttg aaatcgatta
     1381 aggaacctaa tttcatttcg ttcgagaaga ccggcagtct tgagtattca cgagtgccga
     1441 tgtatacgct gataaactca gtgataagtc agaacgatat cgatcatgca ttcgatacca
     1501 tggtagctta ctgcaagaag taccggttca acttcgagtt catgaataca cttctcttcc
     1561 tttccacaag agaaactgac gaactgaaga agctccttca cgttgagggc gagctcgctt
     1621 tgaacgtcct tcacgatatc gttgaaaagg ataaatgggc atcacttata gagaacgaag
     1681 agagtccatc caacaccgaa ctcctgcaag tcatcgaaca ttatgtggat agcttgatcg
     1741 accggaagga ttacagggaa gcactgaggg ttatcgaaga agtctttccc cttgtcggcc
     1801 cggatgatta cggctacctc ttggcgagca aattacttgt gtgctggaga ctgaaaatgt
     1861 tcgatgaatt ggccaaaaca atggagttta ataaccacag tggccatccg aaggctcttg
     1921 cagtattggc gctgaagaac tatgcctaca gggagctaga cagagcggaa cagattcttc
     1981 tgaaaattct tcgccacttc cctgacttca tcaagtatct gctggaggaa gaagaaaacg
     2041 ataagaaggc cttcgagaaa gcagaaagag gcaacctcag gagtcagaaa agagtcgatt
     2101 cgctgactct tgcagaagag ttcagagggg a
//
LOCUS       DAOQYI010000135         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_60951_length_2100_cov_55.706601, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000135 DAOQYI010000000
VERSION     DAOQYI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60951_length_2100_cov_55.706601"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..1437)
                     /locus_tag="PKL93_11450"
     CDS             complement(415..1437)
                     /locus_tag="PKL93_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNQ71916.1"
                     /translation="MMTTSHDYISDRHSIMLLTIRFDAERNNDLKPLNLKNFNNLARR
                     IASSPLSRPSKLLELSEAQIGEQLEIDEKSAKLIAGLLSRKHSLAIELSNLEEKGIRA
                     ISRAEPEYPSKLLDKLKHLAPPLIFYAGDLSILNNKAVSIVGSRKTSQSLLDQAHLLG
                     ERCAQSGVTVVSGAAKGVDMHAMMGSLQNGGLAAGIVADSLKSFIRKDEISRFVRDGR
                     LVLLSAFPPWEKFTVWRAMDRNKYIYSLSDFGVVVRSDLEKGGTWSGATECIEKNYCA
                     LFVLDLGKEERGNAKLIDMRGIPLDIDMISREPDIYRLLSGMIERDSEEEPFIGQNVK
                     QLPLWF"
     gene            complement(1418..>2100)
                     /locus_tag="PKL93_11455"
     CDS             complement(1418..>2100)
                     /locus_tag="PKL93_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71917.1"
                     /translation="DCGRCCNCAGDFRPRATNPQLVQKAAKFLKNRFVRIEPRKRFPY
                     RFEDMIQIPEPFRYEAGYSLSIYGDAGWGRMVKEDKYERGHFRSELVAASKDLITDYW
                     SPEPFPEIVVSVPSLRRPYLVSRFAEALAKEIGLSYIPDCVVKKKETHEQKAMKNSSM
                     QFENVLSAFDVSLDLGGKRVLLVDDLVDSRWTFTVIAYLLKKKNAGAVYPFALSASWE
                     GGYDDDES"
BASE COUNT          587 a          526 c          411 g          576 t
ORIGIN      
        1 gggcattatc aatatgattc actgcttact atgctatatc agcagatagg atgagactcc
       61 tatccttgcc ccgaaaaaaa tctcacttag atctcaacca tatatttcat accagttcca
      121 tcgctctttc ttttcattcg ctgaagtcta aaaaccgaca ggcaaacagc gtgaaatcgt
      181 ttccaagctt gaagcaaaag agtgtccaac ccacttcgaa acctggctta acacctagta
      241 acacgcttac aatcttcaca aactccatag ccctttctgg gaaactaact catgcgtctt
      301 gtttatctat atgatgtcga actcttggaa atagcctgca ataagagtcc agagtatgag
      361 gttgaggtca cagaaaggag ttccattaat gatcagacca atgataggaa gctctcaaaa
      421 ccaaagcgga agttgtttca cattttgacc aatgaacggc tcctcttcac tgtctctttc
      481 gatcatgccg gataacagtc ggtatatgtc gggctcccgg gagatcatat caatgtcaag
      541 cggtatccct ctcatatcta ttagctttgc gtttcctctt tcttccttac cgaggtcaag
      601 cacaaacagt gcgcagtaat tcttttcaat gcattctgta gcccctgacc aagtcccacc
      661 cttttccaga tcacttcgta caactacgcc aaagtcggaa agcgaataga tatatttgtt
      721 tctatccatt gccctccaaa cagtgaattt ttcccaagga gggaacgctg acagcaatac
      781 gagacgacca tccctaacaa atctggagat ctcatccttt cttatgaaac tcttgagaga
      841 atcagctaca ataccggcag caagacctcc gttctgaagc gatcccatca tagcgtgcat
      901 atcaacacct tttgcagcac cggaaactac tgttacacct gactgggcac atctttctcc
      961 caagagatga gcttgatcaa gcaaagattg agaggttttt ctggaaccta cgattgaaac
     1021 cgccttattg tttagtattg aaaggtctcc cgcataaaaa atcaatggcg gtgcaagatg
     1081 cttgagctta tccagcagtt tgctagggta ctccggttct gcacgcgata tcgcacgaat
     1141 gcctttttct tcgagatttg aaagctcaat cgcgagagag tgcttcctac tcaaaaggcc
     1201 agctataagc ttcgcagatt tttcgtctat ctcaagctgc tcgccaattt gcgcttcact
     1261 tagctccagg agcttggaag gcctcgaaag cggagaagag gcaatgcgcc tggcaaggtt
     1321 gttaaagttc ttcaagttga gaggtttgag atcgttgttc cgctctgcat caaatctgat
     1381 tgtaaggagc attatcgaat gcctgtcgga tatgtaatca tgactcgtcg tcatcataac
     1441 caccttccca cgaagccgat aaagcaaagg gataaaccgc accggcgttt ttcttcttca
     1501 gcagataagc tattactgta aatgtccatc tcgaatcgac aaggtcgtcg acaaggagga
     1561 ctcttttacc cccaaggtca agagaaacat caaaagcgga gagtacattt tcaaactgca
     1621 tagatgagtt tttcatggcc ttctgttcat gtgtttcctt tttcttcacg acacaatccg
     1681 gtatgtaact cagaccaatc tctttagcca aagcttccgc aaatctagag accagatagg
     1741 gacgtctgag agacggaacg gacacaacaa tttctggaaa cggttcaggc gaccagtaat
     1801 cggtaatcaa gtccttagaa gcggctacca attcacttct gaaatgtccc ctttcgtatt
     1861 tatcctcctt aaccattcta ccccaaccgg catcaccata aatgctcaac gaataaccag
     1921 cttcatatct gaagggttcc ggtatttgta tcatgtcttc aaatcgataa gggaaccttt
     1981 tccttggctc aattctcacg aatctattct taagaaactt cgcggctttc tgaacgagct
     2041 gagggtttgt agccctgggt cgaaaatccc ccgcacagtt gcagcatcta ccgcaatctt
//
LOCUS       DAOQYI010000136         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_61404_length_2090_cov_50.844717, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000136 DAOQYI010000000
VERSION     DAOQYI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61404_length_2090_cov_50.844717"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2090
                     /locus_tag="PKL93_11460"
     CDS             <1..>2090
                     /locus_tag="PKL93_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ71918.1"
                     /translation="RPVINVSWWDAIAYCNWLSEMEGLPKAYDEEGNFLDIDGELTTD
                     PSRVVGYRLPTEAEWEYAAKGGNKSEGFRHAGSDTISIVAWYDKNSEGITQLVGKKAP
                     NELGIYDMSGNVCEWCSDSFREYSKSSVINPFENNEGYPRVVRGFILGVFFDYASVSY
                     RSVEDYTSRDYIGIRICRSVPYEGMNRPPYEPNNSVPTDQEQGKTTILTLSWSGGDPD
                     GDEVTYEIYLGKDRSPSLIERGLKVLSYVFNDLDVGTTYHWKVVARDSKGEISEGPIW
                     TFTTHVDTSLYKPTHIVPSMVFVEKGSFTMGDIWGDGDPDERPLHEVSITYDFCIGKY
                     EITFEEFDFFSKAAGWSKPNDQGLGRGDRPVINVIWWDAIAYCNWLSEMEGLPKAYDE
                     EGNFLDIDGELTTDPSRVVGYRLPTEAEWEYAARGGNKSKGYKYSGSDNVDDVAWYIS
                     NSGWWTQEVGKKLLNELGIYDMSGNVWEWCSDSYGNYSDSTQTNPYNSTAGSDRVYRG
                     GGVHSDVQETRVTNREDDFWSNYPETGFRICRTLPFALENRSPFEPNHPSPSNNSINQ
                     PTTITLNWECIDPDGDPMSYSIYFDTKANLTTEVASDVTTAKFHRNNLLCRTTYYWKI
                     VARDSKGNITEGPVWRFTTLALTKSMVLVEKGNFTMGDTWGDGSVIERPTHRVMLTYD
                     FYIGKYETTFVEYD"
BASE COUNT          634 a          402 c          523 g          531 t
ORIGIN      
        1 acaggccagt tatcaacgtt agctggtggg acgctatagc ctactgcaat tggttaagtg
       61 aaatggaagg tcttccaaag gcgtatgatg aagaaggaaa cttcttggac attgatggtg
      121 aacttactac cgatccctca cgagtagtgg gatacagatt gcctacagaa gccgaatggg
      181 aatatgctgc aaaaggtggt aacaagagtg aaggattcag gcacgccggc agtgatacaa
      241 tttctattgt tgcttggtac gataaaaatt cagagggaat tacgcaatta gtcggaaaga
      301 aggcgcctaa cgagttaggc atctacgata tgtcaggaaa tgtatgtgaa tggtgttcag
      361 attcgtttcg agaatactct aaatcatcgg taataaatcc tttcgaaaat aacgagggat
      421 acccccgcgt tgttcgtggt tttattcttg gtgttttttt cgattatgca agcgtatctt
      481 acagatccgt ggaagactac acttctcgtg attacattgg tattcgcatt tgcagatcag
      541 tgccttacga gggtatgaac agaccccctt atgaacccaa taactcagtc cctacagacc
      601 aagagcaagg taagaccaca attctgactc ttagttggag tggtggtgat ccagatggag
      661 atgaagttac ctatgaaatc tatttaggaa aagacaggtc gccatcactg atagaaagag
      721 gtctcaaggt actctcatac gtcttcaatg acttagatgt gggaaccact taccattgga
      781 aagtggtagc gagagattct aaaggagaaa tttcggaagg acctatatgg actttcacta
      841 cgcacgtaga cacttcactt tacaagccta ctcatattgt tccttcaatg gtgtttgtgg
      901 aaaagggaag tttcacaatg ggcgatatct ggggagacgg ggaccctgat gaaagaccgc
      961 ttcacgaagt aagcattaca tatgactttt gcataggtaa atatgaaata acatttgagg
     1021 aatttgactt cttttctaaa gccgctggtt ggagcaaacc aaatgaccag ggcttgggaa
     1081 gaggcgacag gccagttatc aacgttatct ggtgggacgc tatagcctac tgcaattggt
     1141 tgagtgaaat ggaaggtctt ccaaaggcgt atgatgaaga aggaaacttc ttggacattg
     1201 atggtgaact tactaccgat ccctcacgag tagtgggata caggctacca actgaagctg
     1261 agtgggaata cgcagccagg ggtgggaata agagcaaggg atacaagtat tctggtagtg
     1321 acaacgttga tgatgttgcc tggtacattt caaattctgg gtggtggaca caggaagttg
     1381 ggaagaagtt gctaaacgag cttggaattt atgatatgtc cggaaacgta tgggaatggt
     1441 gcagcgattc gtatggaaac tactccgatt ctacacagac gaatccgtac aacagcacag
     1501 ctggctccga ccgagtgtat cgtggcggcg gtgtacactc cgatgtgcaa gaaacacgtg
     1561 taacaaatcg tgaggatgat ttctggtcta attatccgga aaccggtttt cgaatctgta
     1621 gaacgctacc atttgcactg gaaaacaggt ctccttttga accaaatcat ccgtcacctt
     1681 ctaacaactc aatcaaccag cccacaacaa taactctaaa ctgggaatgt atagaccccg
     1741 acggagatcc aatgagttac agcatctatt ttgatacgaa ggccaatctt actacagaag
     1801 tagcatctga cgtaaccact gcgaagtttc acagaaacaa ccttttgtgt agaactacct
     1861 attattggaa aatagtggcc agggatagta agggaaacat cacggaaggc cctgtatgga
     1921 gattcacgac attagcgcta acgaagagta tggtacttgt agagaagggg aacttcacga
     1981 tgggtgatac ctggggagat ggatcggtca tcgaaaggcc cacacacaga gttatgctaa
     2041 cctacgattt ctatatagga aagtatgaga caacttttgt cgagtacgat
//
LOCUS       DAOQYI010000137         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_62694_length_2063_cov_75.589641, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000137 DAOQYI010000000
VERSION     DAOQYI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62694_length_2063_cov_75.589641"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2063)
                     /gene="nifJ"
                     /locus_tag="PKL93_11465"
     CDS             complement(<1..>2063)
                     /gene="nifJ"
                     /locus_tag="PKL93_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNQ71919.1"
                     /translation="NPEHPKTMGTAQNPDIFFQAKEASNRFYDAVPEIVADYMKEISK
                     ITGREYKPFVYYGDPEAEHVIIAMGSVTDTIEETVDYLMKKGEKVGVIKVHLYRPFSA
                     KHFFEVLPNSVKRISTLDRTKEPGSLGEPLYEDVKTLFYGDKNAPEIYGGRYGLGSKD
                     TTPSQIKAVYDNLKLSTPKDHFTIGIVDDVTNTSLEIKERINTAPEGTIRCKFWGLGS
                     DGTVGANKDAIKIIGDHTDMYAQGYFAYDSKKSGGVTISHLRFGKKPIKSTYLIDEAD
                     YVACHKQSYVYQYELLEGLKKGGTFVLNTSWDSEELDRNLPGNMKKYLAENNIDFYTI
                     DATKIAMEIGLGTRINTIMQSAFFKLANVIPIEEAIKYLKEAIVKSYGTKGEKVVQMN
                     YKAVDSGIDALKKIDIPESWAKAQDEKKEEDSERPEFVRKVADVMNRQQGDKLPVSAF
                     LDRENGEFPNGTAAYEKRGIAVLIPEWQIDNCIQCNQCSLVCPHAAIRPFLLNEEEEN
                     RAPDTFEAKKGLGGKPFEGLKYRIQVSPLDCTGCGNCADICPAPNKALIMKPLESQVE
                     REVPNWEFATTVSEKKNVMNVETLKGSQFSKPLIEFSGACAGCGETPYAKLVTQLFGD
                     RMLIANATGCSSIWGASAPSTPYCMNGEGKGPAWANSLFEDNAEYGFGMAMAVKHGRN
                     KLAKI"
BASE COUNT          484 a          513 c          425 g          641 t
ORIGIN      
        1 ataatctttg caagcttgtt tctaccgtgt ttaactgcca tagccatacc gaatccgtac
       61 tcagcgttgt cttcaaacag tgaattcgcc caggcaggcc ccttgccttc gccgttcata
      121 caataggggg tcgaaggagc cgaggctccc cagattgaag aacatccagt cgcgttggct
      181 ataagcatcc tgtcaccaaa gagctgggtt actagttttg cataaggggt ttccccgcaa
      241 ccagcacagg ctccggagaa ctcaataaga ggcttagaaa actgactgcc tttcaatgtc
      301 tcgacattca ttacattctt tttttctgag acggttgtag caaattccca gtttgggacc
      361 tctctctcaa cctgtgattc gagtggtttc attatcagag ctttgttcgg agccggacaa
      421 atatctgcac agtttccaca acctgtacag tcaagaggcg aaacctgaat cctgtacttc
      481 agcccctcaa aaggcttacc gccaagccct tttttggctt caaaagtatc tggagctcta
      541 ttttcttctt cttcatttaa caggaaaggt ctgatcgcag cgtgtggaca gactagagaa
      601 cattgattgc actgaatgca gttgtcaatc tgccattcag gtatcagaac agcgatgcct
      661 cgtttttcgt atgccgctgt tccgttgggg aactctccat tttccctatc taggaaagca
      721 gaaaccggta gtttgtctcc ctgctgcctg ttcataacat ctgcaacttt cctaacgaat
      781 tcaggccttt cagagtcttc ttcctttttc tcatcttgtg cctttgccca tgattctgga
      841 atatctattt tcttcaacgc atctattcca ctatctactg ctttgtaatt catctgtacc
      901 actttttcgc ctttggtacc gtaagacttg actattgctt ctttcagata cttgatagct
      961 tcttctatag gtattacatt ggccagcttg aagaaggcag attgcattat cgtattgatt
     1021 ctcgttccaa gaccgatctc catggcaatt ttcgtggcat cgatcgtgta gaaatcaata
     1081 ttgttttctg caaggtactt tttcatgttg ccgggaagat tcctgtcaag ctcttcagaa
     1141 tcccagctgg tgttaaggac aaacgttcca cccttcttaa gtccctccag caactcgtac
     1201 tgatacacat atgactgctt gtgacaggca acataatccg cctcgtcgat caggtaggtt
     1261 gactttattg gcttcttgcc gaatctaaga tgcgatatgg ttactccacc agacttcttt
     1321 gaatcataag cgaaatatcc ctgggcatac atgtcggtgt gatctccgat tatctttatc
     1381 gcgtccttgt tagctcccac cgtaccatcg gaaccaagtc cccagaactt gcaacggata
     1441 gtaccttctg gagcggtatt gatcctctcc ttgatctcga gagaagtatt ggtgacatca
     1501 tcgactatac ctatcgtaaa gtggtccttt ggcgtcgaaa gtttcagatt atcgtagaca
     1561 gctttaatct gagaaggagt ggtatccttt gaacccagtc cgtatctgcc accgtaaatt
     1621 tcaggagcat tcttatcccc atagaagaga gtcttcacat cttcgtatag aggttcccca
     1681 agagagcccg gctcctttgt tctgtctaga gtagaaatcc tcttgactga atttggaaga
     1741 acctcaaaaa agtgctttgc agagaacggt ctgtaaaggt gtacctttat tactccgacc
     1801 ttctcaccct ttttcattag gtagtctact gtctcctcga ttgtgtcggt gactgagccc
     1861 atcgcaataa ttacgtgctc cgcttctgga tcgccgtagt agacgaaagg cttgtactcc
     1921 ctgcctgtta tctttgaaat ctccttcatg taatcggcga cgatttctgg aaccgcatca
     1981 tagaatctgt tcgaagcttc cttcgcctgg aagaagatat cgggattctg ggccgttccc
     2041 attgttttgg gatgctctgg att
//
LOCUS       DAOQYI010000138         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_63402_length_2049_cov_56.184554, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000138 DAOQYI010000000
VERSION     DAOQYI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63402_length_2049_cov_56.184554"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(670..1020)
                     /locus_tag="PKL93_11470"
     CDS             complement(670..1020)
                     /locus_tag="PKL93_11470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNQ71920.1"
                     /translation="MNRELPSRGEIWLANLSPTRGREQSGFGTCLVISVDQFNHGPAE
                     LVIVVPLTSKNKSIPLHVEISGEQTGLDVTSYIKTEDLRSISRNRLEKKIGQVSEEVI
                     LDVLDRIKILLDIT"
     gene            complement(1013..1264)
                     /locus_tag="PKL93_11475"
     CDS             complement(1013..1264)
                     /locus_tag="PKL93_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71921.1"
                     /translation="MKTQIVRVPFETHSRLKAMASASGETIGEILAKAVESYRRELLL
                     EDTNEAFSKLKEQADLWKGELDEREEWEGSLLDGQSNHE"
BASE COUNT          515 a          473 c          475 g          586 t
ORIGIN      
        1 ctggaggaac cgcttgtttg aaacgctgta ggctaatcgc tcagaaaaac aataacaagt
       61 gcatgattct gaatcggatc attgatcaag aggatcgtct accggctctt cacgttcttc
      121 ctgtgcagca tatcttttgc tcctctcgct cggagttcgg agaatcgttt acggtgaacc
      181 tgcaattgtc tcctgccgaa ggcgtaacgg acggggacag acgtcgggag ctcgtcagtc
      241 ccctattcga aatgctacgg gctgacaatt acagatgatt atccggttct tttgtagggg
      301 cgaacggctg ttcgcccgaa aaaggtcctg gttggaatct ctgcctttac cgcgaaaaaa
      361 gggcagacct taggaactgc tcagtcccca ttttcgccct cgccttctct tgagcgccgc
      421 gaactctcta atatgctctt tctcggctac tcacagcctt gcaacatgac acttggaact
      481 gttcttccca gcgtacagcg gcactttgag atggcagttc atatagggat tctcttaggg
      541 gaaaactttg cccagattca ccagaatcta tcacatgaat gcctacgaga aatcaacctg
      601 gccactagaa aatcttctat agactaggat acgagctctt gttgcagcag gtattctccc
      661 tcaatcgttt catgtgatat ctaggagaat ctttatccga tccagaacgt cgaggattac
      721 ttcttctgag acctgaccga tcttcttctc caatctattt ctcgagattg accttaagtc
      781 ctcggtctta atgtagcttg ttacatcgag ccctgtttgc tctccggaga tctcaacatg
      841 cagcgggata gatttgttct ttgacgttaa gggaacgaca atcacgagct cagcaggtcc
      901 atgattgaac tggtcgaccg aaatgaccaa acatgttcca aatccagatt gctctcttcc
      961 tctagttgga ctgaggtttg ccaaccagat ttctcctctt gaaggtagct ctctattcat
     1021 gattactttg cccgtctaat agtgagcctt cccattcttc cctctcgtct aactcgcctt
     1081 tccaaagatc ggcttgctct tttagttttg agaaggcttc atttgtatct tccaggagca
     1141 actcacgtct atatgattcc actgctttag caagtatttc tccgattgtt tcaccactgg
     1201 cggaggccat tgccttaagc ctcgaatgag tttcgaaagg gactctgact atttgcgttt
     1261 tcaagattga tcaccaccta gtaaattgat tagtatacta taacataaca cgatttgtga
     1321 cagaaaacac cttttcgatt gattcgatcg aagggaatag atgcggaaac gagttcatga
     1381 cggaattgat agcgggtctt gagttagggt ctagggtgta gtgaaagaaa cgatcttctc
     1441 tgcagtcatt ccatggataa tgctttgatg agctccacag gatcccattt ttctcgttct
     1501 tctcattgtt tctcttcttc ttgcaaagtc ctcgtttttc gctatgaatc gagattctgg
     1561 ccagaattat gccagaatga cgggaaggac cgtcatcccg gacttgatcc gggatctggg
     1621 cttgtgagaa acgtgatcgg tggtagcagg tggcgggttc gtaagagcaa gagaaactgg
     1681 ttgggcgttg atcggttttc cgttatccgg ggaaaagctg aaggtgacta aaagaaagga
     1741 tcgagattct gaaacaagtt cagaatgact gtatgtgacg tattcggaga accctccggg
     1801 atgctgaaac aagttcagca tgacgaaatc tcggacgaat aaccaagaac ttggacgcgt
     1861 agcgtcggaa cgaagaaccg tttttctcaa agggcgactc tagtttcagc ataacggcat
     1921 cagaagtttt tttcttggct tgcaacttgg aacagtcaac acctaactgc tcttcgaagg
     1981 acgggttcat ggttttggac gaagggcttc tactatgtca acagttataa aaaaggcaga
     2041 ctgaaagga
//
LOCUS       DAOQYI010000139         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_63705_length_2043_cov_50.962274, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000139 DAOQYI010000000
VERSION     DAOQYI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63705_length_2043_cov_50.962274"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..620
                     /locus_tag="PKL93_11480"
     CDS             21..620
                     /locus_tag="PKL93_11480"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ71922.1"
                     /translation="MATFEQLLKRLEKNEGYQKAQKEFGPLFDFVNALIDLRIEKGLT
                     QKDLSDLTGIAVPTLSRIEAGKQNLSFKTMQTLVNALGGKLCITPKADSVVELNCKAK
                     ESLDRVVEKTGMGKSELIEGLISVYEQVLDGAGKHSIELMKTYQLMAETDYVGALAMT
                     FRSGTESLSSLLTDDFFPSQFEENDELSMDKIEDYFTRG"
     gene            632..1081
                     /locus_tag="PKL93_11485"
     CDS             632..1081
                     /locus_tag="PKL93_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71923.1"
                     /translation="MPEKQELRLNKDEFVEFRTLIDNIEIRDVITVKQAFEANRKQKV
                     TGSVQVNIESAPAEINIEDQLTVLKSYRIVVKQGKKNVLVFSATYEVSFDAKNAEKVN
                     SILEKENIRQFFEGLQLSKILWPFIREEFRTASSKAGMKPFTLPFMK"
     gene            1135..>2043
                     /locus_tag="PKL93_11490"
     CDS             1135..>2043
                     /locus_tag="PKL93_11490"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNQ71924.1"
                     /translation="MSSAFQSREASGGFYLTYLRELREKIVTSSQVVLPDLRALSVFS
                     IKKLENKTKVHNKEIQPLLAVYRNILARGEPTIPSLLVERTILSSLQPLVKVFESNRA
                     GSIKFGPETTYKNCTEEEWMKQLARAHYLIDPRLKNPKESLNGVFDSEEEKTFLASTI
                     PELLGKHAWQLFEPQRPLEQMIGNGEAFSRQKVDFSYESDAWKLAFEIDGEQHLNSSQ
                     SALDNRRSLELREAGWTELRIPASQVRQGAVSSRLSNISKTLAQDPLVKTTYENFTNP
                     LWQSATGLAALNAVLSPVAVARIQVAL"
BASE COUNT          669 a          390 c          465 g          519 t
ORIGIN      
        1 attaggaggg tgtgagtaat atggcaacat ttgaacaact tctcaaaagg cttgagaaaa
       61 acgaggggta ccagaaagca cagaaagaat tcggaccgct attcgatttt gtaaacgcgc
      121 ttattgattt gcgcatcgaa aaaggtttaa cacaaaagga tctaagtgat cttacaggta
      181 tagctgtgcc tactctttca cgcattgaag ctggtaaaca gaatctttca ttcaaaacta
      241 tgcaaacact agtcaatgct cttggtggaa aactgtgcat tacaccgaaa gcggactcag
      301 ttgtcgagct taactgtaag gcgaaagaat cacttgatag agtcgtcgag aagactggaa
      361 tggggaaatc cgagcttatt gaaggcctta tatctgtcta cgagcaggtt ctagacggtg
      421 caggaaagca cagcatagaa ttaatgaaga cttatcagct tatggcagaa acggactatg
      481 ttggggcact tgcaatgacg tttagaagtg gtactgaaag tctatcttcg ttattaacag
      541 atgacttctt cccgtctcag tttgaagaaa acgatgaact gagtatggat aaaattgaag
      601 attactttac gagaggctga ggagagcgct tatgcctgaa aaacaggaat taagactcaa
      661 caaagatgag tttgtggaat ttagaactct tattgacaat atagagataa gagacgtaat
      721 cacagtcaaa caagcttttg aagctaacag aaagcagaaa gtaactggat ctgtacaggt
      781 taatatagaa tcagcacctg cggaaatcaa tattgaagat caattaactg tgttaaagag
      841 ctatcgaatc gtggttaaac aaggaaagaa aaatgttctt gtcttttcgg cgacttacga
      901 agtttcgttc gatgctaaaa atgcggaaaa agtaaacagc atactggaaa aagagaatat
      961 ccgacagttt tttgaaggcc tgcagttgtc taaaattttg tggcctttca ttcgtgaaga
     1021 gtttcgaaca gcttcctcaa aggccggtat gaagccgttt actcttcctt ttatgaagta
     1081 aacaggagtg tttcggattg cgatgacata aactaagtat agggggaaga ggtcttgtca
     1141 tcagcgtttc aaagcagaga agcgagtggg ggtttctatc taacctacct tcgtgagctt
     1201 cgagagaaaa ttgttacttc ttcacaagta gttctgccag atttaagagc actttcggtc
     1261 ttcagtatca agaaacttga aaacaaaaca aaggtacata acaaggaaat ccagccatta
     1321 ctagccgtgt atagaaatat cctcgctcgt ggcgagccca ctattccatc tcttctagtt
     1381 gaaaggacaa ttctttcgtc tcttcagccc ttagtaaagg tttttgaaag caacagagcc
     1441 ggttctataa aatttgggcc ggaaacaact tataaaaact gtacggaaga agaatggatg
     1501 aaacaactgg ccagagccca ctatctgata gatccaagac tcaaaaaccc gaaagaaagc
     1561 ttaaacggag ttttcgatag cgaagaagag aaaaccttct tggcctcaac aattcccgaa
     1621 ctactgggca aacacgcctg gcagcttttt gaaccccaga gaccactgga acaaatgata
     1681 ggaaatggag aagccttttc tcggcaaaag gttgattttt cttatgaatc agatgcctgg
     1741 aaattggcat tcgagattga tggagaacag caccttaata gttctcagag tgccttggac
     1801 aacaggagga gtttggagct tagggaagca ggatggactg aactaagaat cccggcctcg
     1861 caagttcgcc agggcgcagt ctcgagcaga ctttcaaata tttcaaaaac tctggcacaa
     1921 gaccccttgg tcaaaactac atatgagaac ttcaccaacc cgctttggca atcagcgaca
     1981 gggttagctg ctttaaacgc agtcctcagt cccgttgcag tagcaagaat tcaggtagcc
     2041 ctt
//
LOCUS       DAOQYI010000140         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_64188_length_2033_cov_74.547523, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000140 DAOQYI010000000
VERSION     DAOQYI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64188_length_2033_cov_74.547523"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            538..999
                     /locus_tag="PKL93_11495"
     CDS             538..999
                     /locus_tag="PKL93_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ71925.1"
                     /translation="MEHIVRFEEITEETVLDAIRLSGTLLKGQERAVASNAVSIAQAH
                     FAKNAWFRAIYAGDDMVGFIMLDFTPDPRMKDVKHASMWRFMIGGKYQKMGYGRDAIL
                     LLIEYLKAQGYEKLFTSCIVNDPSPLHFYLKLGFVDTGEWDDDEKILMRDL"
     gene            complement(996..1781)
                     /locus_tag="PKL93_11500"
     CDS             complement(996..1781)
                     /locus_tag="PKL93_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71926.1"
                     /translation="MISSAKNIILSTASIIADIISGNWSGVAQQISIEALLDEFVDYV
                     KNDLLDDVLEAVKDKLRAYLSDPSKAEELLSLVKGDMLGWVKDGEDSDEGSVRDEIRQ
                     VVYTDLTYRNFTKPVIRELEESLSGAARLASLSISDLDLYAASWSMNRDFASMRSEVM
                     GPLQDWSFEVLGEHERIDNWETILEIFEETVPLIVELLRLMEYKNPVLGDIAEELSKL
                     SYVLDAVGLLTLAYEVSLKADQLTTMAGKLDRVNDYLFLEAGY"
     gene            complement(1813..>2033)
                     /locus_tag="PKL93_11505"
     CDS             complement(1813..>2033)
                     /locus_tag="PKL93_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ71927.1"
                     /translation="EENRQSIQVSMQLRKLSRHDFLKLPHFVIPNSFRDPGLILSEDG
                     GPLTDNVAFSSDQRVFVLKRAAALRRGC"
BASE COUNT          533 a          511 c          483 g          506 t
ORIGIN      
        1 aaataatgtc ctctatcgct ctccaactct cacctcagca tttggttagc aacttcccgc
       61 tcaatccgat aataagaagt ataccatccg ataattctac tataagcgaa ccgttacccg
      121 cagagatctg aatcaccccg gttcgaagca atgaaaaacg gatcgagatc tgccaggatt
      181 attgagccga ctaaatactt ttcatgtgag gatgaaacga ttagaaagac cggaagcctg
      241 cataaaccac tggaggatgt ctgcagcgct aacccagtta cgaagcgctc tgtgaatgtg
      301 gccggatgtt ccttctaaat gttcagggat acaagaagtg gaacccaaaa gcagctgctg
      361 agagaaaaca atttgtgcag gggcatgatg tggcttgatt gcagctcaat gatataatgc
      421 agcaaggaag agtgttttcg gaaaggccaa cctgccaaga atgttttcgg cgcggctcca
      481 atatttggtc tttgtttcga ctagagcgaa atatatcgct agaaatcagg aggagtcatg
      541 gaacatatag ttagattcga agagataaca gaggagacgg tactcgacgc tatccgtttg
      601 agtggtacct tactcaaagg acaggaaaga gccgtggcct caaatgcagt ttcgatcgct
      661 caagcccact ttgcaaagaa tgcctggttc cgtgcaatct atgcaggaga tgacatggtg
      721 ggcttcatta tgctggactt cacacccgat ccgagaatga aagatgttaa gcacgcctcc
      781 atgtggcgct tcatgatcgg cggcaagtat cagaagatgg gatatggaag agacgctatc
      841 ctcttgttga ttgagtatct caaggcccag ggttacgaga agctcttcac ctcttgcata
      901 gtcaatgatc cttccccgct ccacttctac ctgaagctgg gcttcgtcga tactggggag
      961 tgggacgatg atgaaaagat actgatgaga gacctctagt aaccggcctc gagaaagaga
     1021 tagtcgttga ctctatcaag ctttcctgcc atcgttgtga gctgatcggc tttcaatgac
     1081 acttcatatg ccagagtaag gagacctacg gcatccagca cataagagag cttcgacagc
     1141 tcctctgcaa tatcgcctag aacggggttt ttgtactcca tgagcctcag aagctccaca
     1201 atcagaggaa ccgtctcttc gaagatctcg aggatcgttt cccagttgtc gattctctca
     1261 tgctctccga gaacctcgaa cgaccagtct tgcagaggcc ccatcacttc acttctcatg
     1321 cttgcgaaat ccctgttcat actccagctc gcagcgtaga gatcgagatc tgaaattgac
     1381 agcgaagcca ggcgagccgc ccccgacaga gactcctcaa gctctcttat taccggtttt
     1441 gtgaaattgc ggtaagtgag gtcggtatag actacctgcc ggatttcatc cctgacgctt
     1501 ccttcatccg agtcttcgcc gtcttttacc catccgagca tgtcaccctt tactagactc
     1561 agaagctcct cggcctttga aggatcggaa aggtatgccc tgagcttatc tttcacggcc
     1621 tcaagcacat cgtcgagcag gtcgtttttc acataatcta cgaactcatc cagcagtgct
     1681 tcgattgata tctgctgtgc cacgccgctc cagttacccg agataatgtc ggcgataatc
     1741 gaagcggtgg agagtattat gttctttgca gaagatatca ttgcggccac ttcctctgca
     1801 tctgaacttg tttcagcatc cccttctaag agcagctgca cgcttaagaa cgaagacccg
     1861 ctgatcgctg ctgaaggcta cgttgtccgt cagcggtcca ccgtcctcag agaggatcaa
     1921 acccggatcc cgaaacgagt tcgggatgac aaagtgaggg agtttcaaaa agtcatgccg
     1981 ggataacttc ctcagttgca ttgaaacctg aattgattgc cggttctctt cac
//
LOCUS       DAOQYI010000141         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_64407_length_2029_cov_64.027356, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000141 DAOQYI010000000
VERSION     DAOQYI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64407_length_2029_cov_64.027356"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..632)
                     /locus_tag="PKL93_11510"
     CDS             complement(174..632)
                     /locus_tag="PKL93_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71928.1"
                     /translation="MDKMQPLMNSSLGPCIKNGLQAIAKPDKVSLQVTRTKAKSVDID
                     RCLEEDYPNDNRWDYAVFIETDDILNTAFIEIHPANESEVDAVIKKAQWLKRWIVDND
                     LLIITENRKFFWVSSGKVKVSKNSQKIRLLRKQGIEGPQEHLVVDKSMKD"
     gene            complement(636..1787)
                     /locus_tag="PKL93_11515"
     CDS             complement(636..1787)
                     /locus_tag="PKL93_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ71929.1"
                     /translation="MRVVLENIGPIEKADVSAGDLTVLVGPQATGKSIFLQLFKFCVD
                     HANIVRELKKLGFDWNNELESFLPVYFGEGMQAIWNSQSRIELNGRKFQIETHVKRSG
                     KADSEAKVFYIPAQRVLIISAGWPLPFNSFDPTYPFVVKDFSEKIRQIMGSRLGSGAT
                     PIFPRDGYFKKEIRDALQKSIIREGILKLDIGLRKRIMLEIAGVSMPIMTWSAGQREF
                     APLLLGLFWLLPSAKVSRKQGVNWVIVEEPEAGLHPLAIRDVFLTIVELLSRNYKVLL
                     STHSAGIIELVWAINEIVSHPEASTDKKRRLLLDLFELKTTPNTNKLIKDLENKKLVV
                     SYFKPTETGVVTEDISSLDPGSDKKSVSEWGGLVEFTSKTNEIVAQLWR"
BASE COUNT          531 a          465 c          413 g          620 t
ORIGIN      
        1 gtaacggaga tcttcattca ataggaggaa cttactggca gtggttctcc gttctctcag
       61 aagagcgaga tctcgcataa aatcaccata ggatgaccaa atgaggagat gttaacttac
      121 gcattttctt aggtagtgcc tttgaagacg aataagacaa cctcaaagat gtttcagtct
      181 ttcatgcttt tgtcaacaac taaatgttct tgtgggcctt cgattccttg tttgcggaga
      241 agacggattt tctgagagtt cttgctgacc tttaccttgc ctgatgaaac ccagaagaac
      301 ttccggttct cagtgatgat caacaggtca ttatcgacaa tccatcgctt cagccattgc
      361 gccttcttga tcaccgcatc gacctcggat tcatttgccg gatgaatctc tatgaatgct
      421 gtattcagaa tatcatcagt ctcaatgaag acggcatagt cccacctatt atcattggga
      481 taatcttctt caagacatct atctatgtca acgctctttg ctttagttct cgttacttga
      541 aggctgactt tgtcaggttt cgcaattgct tgaagtccgt tctttataca ggggccaaga
      601 gaagaattca tcaagggctg cattttgtcc attttctacc tccagagctg tgcaacgatt
      661 tcgtttgtct tggaagtgaa ttctacaagg ccaccccatt ctgaaactga cttcttatcg
      721 ctcccgggat ccagtgaaga aatatcttca gtcaccacac cggtttcggt tggtttgaaa
      781 tagctgacta ctagcttctt gttctcaaga tcttttatta gtttgtttgt atttggggtt
      841 gttttcagtt cgaaaaggtc gaggagaagt ctcctctttt tgtctgtact tgcctcagga
      901 tgagatacaa tctcatttat cgcccaaacg agttcgatta ttcccgcaga atgggttgag
      961 agcaagactt tgtaatttct gctcaacagt tcgactattg tgagaaagac atccctaatt
     1021 gcaagcgggt gaaggcctgc ttccggctct tcaacaataa cccagttcac accttgtttc
     1081 ctcgatactt ttgctgaagg caacagccag aataatccaa gtagcaaggg cgcaaattcc
     1141 ctttgacctg cggaccaggt cattatgggc attgatactc ctgcaatctc cagcatgatt
     1201 ctttttctga ggccgatgtc tagttttaat attccttctc ttattatgga tttctggagt
     1261 gcgtctctta tctccttctt gaagtatccg tctctcggaa agatcggggt agcgccgctc
     1321 ccgagacgcg aacccataat ttgcctgatc ttctcactga aatccttaac aacgaaaggg
     1381 tacgtaggat cgaaagagtt gaaaggaagg ggccacccgg cagagataat caggactcgt
     1441 tgagcaggta tataaaatac tttggcttca ctgtcagctt tacctgatct cttcacgtga
     1501 gtttcgatct ggaacttcct cccgttaagt tcaatgcgac tctgtgaatt ccatattgct
     1561 tgcattcctt cgccaaaata gacaggcaaa aaactctcga gctcattatt ccaatcaaaa
     1621 ccaagcttct tcaattccct tactatattg gcgtgatcaa cgcagaattt gaacaactga
     1681 agaaagatgc tcttccccgt tgcttgcggt ccgacgagaa ctgtcaaatc acctgccgaa
     1741 acgtctgctt tttctatcgg accaatattc tcaagtacga ctctcacggt tggccctcct
     1801 ttcttcttcc tctaatcgat atcctattta aggtaattat aacaacctcc gcatgaattc
     1861 gcgcattttc aactgaagca gaaaaacggt gacaagcgga gatttgaacc tctgtgaaga
     1921 ttattcattt cgagcgaaga agaataagcg atgttagaaa atcagcgata tgtcttttgc
     1981 tataatctga agtgtgcaaa aatgggtaca gacattagaa gacttgtca
//
LOCUS       DAOQYI010000142         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_68251_length_1959_cov_57.495273, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000142 DAOQYI010000000
VERSION     DAOQYI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68251_length_1959_cov_57.495273"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKL93_11520"
     CDS             complement(<1..906)
                     /locus_tag="PKL93_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNQ71930.1"
                     /translation="MKILAGAIGSDIHTAGILNFLNLARNEGYETIYIGSVIGIDKLL
                     DSIEEASPDIVAVSYRLGKESCEQLLRELKVSLENRGIEKRLIFGGTVETAEVAEKSG
                     LFEKVFDGSEMPEEVVMFLRGKTDEKGEVDYPQTLLERIASKKPYPLIRHHIGLETVD
                     ATEEAVRKLAESGLLDIISLAPDQNCQQWFFQQENMISSEDGAGGAPFRKREDFERMY
                     AASRTGNYPLMRCYSGTRDLLKFSLLFKETLNNAWAAIPISWYSQLDGRSDRELLEAI
                     RDNQAAIAWNGGKGIPVEINEAHQWA"
     gene            complement(908..>1959)
                     /locus_tag="PKL93_11525"
     CDS             complement(908..>1959)
                     /locus_tag="PKL93_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HNQ71931.1"
                     /translation="GGQALSAVLAMRGHDVSLYNRSQKRISPLLLERKIRVEGESEGK
                     AKLRYVGTDMKKAIEGAEAIMVVVPAFAHAKIAEKLAECIGEGQIVVLNPGRTGGALE
                     FDKIFRAKGVREKAVIAEAQTFVFASRISGPGMVRIFRIKNAVPLSVLPSKDNESVFP
                     IISEIMPEFTLAKNVLYTSFNNIGAVFHPGAILMNAGWIETKHGDFQFYLEGISPSVA
                     RVLEALDKERCEVTEKLGVEAMGAKEWLDYAYDVKGEDLYAAIHSNEGYRGIMAPISI
                     ENRYIMEDVPMSLVPMSCFGRELGVDTRVMDSIISIAGAVMGRDFWKEGRNLVSLGVD
                     EMSVEELTRYVEEGT"
BASE COUNT          421 a          575 c          410 g          553 t
ORIGIN      
        1 cgcccactga tgggcctcgt ttatctcaac cgggattccc tttccaccat tccaggcgat
       61 tgcggcctgg ttgtctctta ttgcctccag aagttcacga tctgatctcc catcgagctg
      121 agagtaccag ctaatgggta tcgcggccca cgcgttgttc aaggtttctt taaagagaag
      181 ggagaatttt aggaggtctc tcgttccaga ataacacctc atcaatggat agttaccggt
      241 cctgctggca gcatacatcc tttcaaaatc ctctcgtttt ctgaaaggcg cgccccccgc
      301 cccgtcttcc gaggagatca tattctcctg ttgaaagaac cactgctggc agttctggtc
      361 cggagccagt gagatgatat ccagcaagcc cgattcagca agctttctga cggcttcctc
      421 tgttgcatcg accgtctcaa gccctatgtg atgtcttatc aacggatatg gctttttgga
      481 ggcgattctt tcgagaagtg tctgaggata gtcgacctct cccttttcgt ccgtcttgcc
      541 cctgagaaac atcacgactt cctccggcat ctcgctgccg tcaaagacct tctcgaagag
      601 accgctcttt tcagccacct cggccgtctc gaccgttcca ccgaagatca gtctcttttc
      661 tattcctctg ttttccagcg agactttcag ttctcggagg agctgctcgc aggactcttt
      721 tcccagcctg tagcttacgg ccactatgtc cggcgaggcc tcttctatgc tgtctagcaa
      781 tttgtcaatg cctatcacgc ttccaatgta gatcgtttca tagccttcat tacgcgccaa
      841 gttcaagaag ttaagaattc ccgctgtgtg aatatcgctt ccaatggccc ctgcaagtat
      901 cttcatatca ggtcccttcc tcgacatacc tggtcaattc ttctacggac atttcgtcga
      961 cgccgaggct cacaagattt cttccttcct tccagaagtc tctccccatt actgccccgg
     1021 ctatgcttat tatcgaatcc atgactctcg tatccactcc aagttctctt ccaaagcagc
     1081 tcattggaac cagactcata ggcacgtctt ccataatgta cctgttctca atacttatag
     1141 gcgccatgat tcccctgtag ccttcgttgc tatggatggc cgcatagaga tcttcaccct
     1201 ttacgtcata ggcgtaatca agccactcct tcgcacccat ggcttctact cccagctttt
     1261 ccgttacttc gcagcgctct ttgtccagag cctccaggac tctcgcaacc gaaggactta
     1321 taccttcaag atagaactgg aagtccccat gtttcgtttc gatccagccg gcgttcatga
     1381 gaatcgcacc gggatggaag acagcgccaa tattgttgaa gctcgtgtat agaacattct
     1441 ttgccagcgt gaactcgggc ataatctcgc tgatgattgg aaatacggac tcgttatcct
     1501 ttgagggaag cacagaaaga ggaacggcat tcttaatcct gaatattctc accattccag
     1561 gccccgaaat tcttgaagca aagacgaagg tctgagcctc ggcgatcacg gccttttctc
     1621 taactccctt tgctctgaag atcttgtcga attccagcgc cccgcccgtt cgcccggggt
     1681 tcaaaacgac tatctgacct tcgccgatac attcggccag tttttcggcg atttttgcat
     1741 gagcaaaggc cggcacaaca accatgatag cttcggcacc ctctatcgcc ttcttcatat
     1801 ctgtaccaac gtatctaagc tttgctttgc cctctgactc gccttcgact cttatctttc
     1861 tttctaagag taaaggtgag attcgtttct gacttctgtt gtatagcgag acatcgtgac
     1921 ccctcatggc tagaacggca gaaagggcct gtccaccat
//
LOCUS       DAOQYI010000143         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_68772_length_1950_cov_89.321900, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000143 DAOQYI010000000
VERSION     DAOQYI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68772_length_1950_cov_89.321900"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKL93_11530"
     CDS             complement(<1..229)
                     /locus_tag="PKL93_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71932.1"
                     /translation="MTRRAGIDYRICCLVKETGMTRRAGYRLKGMGSREENLQSIQGF
                     NATGEVIPTSALGSFSLCHPELVSGSRFFEER"
     gene            542..1102
                     /locus_tag="PKL93_11535"
     CDS             542..1102
                     /locus_tag="PKL93_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Thrombospondin type 3 repeat protein"
                     /protein_id="HNQ71933.1"
                     /translation="MKKIVLITITVLLIGGMTLAVGYGTKAMQRVQDTTCENFVDADG
                     DGINDNCPVDGEKVYMNRKGQAGNEAATRAMQRVQDPTTCENFVDADGDGINDNCPVD
                     GEKVYMNRKGQAGNEAATRAMQRVQDPTTCENFVDADGDGINDNCPNDGVRELKNAQD
                     GTGYRHGASGEKGPGLGRGFGKAAQL"
     gene            complement(1620..>1950)
                     /locus_tag="PKL93_11540"
     CDS             complement(1620..>1950)
                     /locus_tag="PKL93_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNQ71934.1"
                     /translation="IVISIELGRETKIIEEDGEKNCLLELTLTAFGRADEKDYLMIKA
                     SMDGIFECQETLEEKALEKIVAESGTSILLPVLRAAILSFTGQAGLTPPIVIPLMNPR
                     FSSEAEE"
BASE COUNT          555 a          449 c          462 g          484 t
ORIGIN      
        1 cacgctcttc aaaaaaccta gatcccgaaa caagttcggg atgacacagt gagaagcttc
       61 ccaaagccga tgtcgggata acttccccag ttgcattgaa accctgaatt gattgcaggt
      121 tctcttcacg agaccccata ccctttaatc tataccctgc ccttcgcgtc atgccggttt
      181 ccttcacgag acagcatatc ctgtaatcga tacctgccct tcgcgtcatg ctgaactcgt
      241 ttcagcatct cgctcctaag aaccgctgtg cgtttaggaa caagaatccc ctgatcgctg
      301 gaagagccgc ttcgtaggaa ccgctttttg ctggcctctg cgaagtacgt tctctcttcg
      361 tcactgaagt gctcctgttc agggatactg tacgtggccc cgttcttgcc agactctaaa
      421 ttccacaacc caagcccccg ctcttcaaat ctctaagcaa tctctaagcg gctgttaagc
      481 gggctctaag aaacgggccg tagactgaga atgtaccaaa aatcatatgg aggtgtaaga
      541 gatgaaaaag atagtgttga ttacgataac ggttcttctg atcggtggaa tgacattggc
      601 agtaggctat ggaacgaaag cgatgcagag agttcaggat actacttgtg agaacttcgt
      661 ggatgcagac ggcgacggaa tcaacgataa ctgccccgta gacggtgaaa aggtttacat
      721 gaatagaaaa ggacaggctg gtaatgaagc agcaacaaga gcaatgcaga gagttcagga
      781 tcctacaacc tgtgagaact tcgtggatgc agacggcgac ggaatcaacg ataactgccc
      841 cgtagacggt gaaaaggttt acatgaatag aaaaggacag gctggtaatg aagcagcaac
      901 aagagcaatg cagagagttc aggatcctac aacctgtgag aacttcgtag atgcagacgg
      961 cgacggaata aatgacaact gcccgaatga cggtgtaaga gaactgaaga atgcccagga
     1021 cggaacaggc tacaggcacg gagcctctgg cgaaaaggga ccgggactcg gcagaggatt
     1081 cggaaaagca gcacagctgt aaaacaaaac ccctactaat tcaagtcaag ggccctaaag
     1141 ggcccttgtt cttataatga tgaaatcgac tttgacagca cgaatcctga aacgcgctaa
     1201 agaagagcaa aattgaaagt aaatccagac gtaccaccgc attttcaaat caccgcgcgc
     1261 ttgactcgtt gagtatctca aaggccccag actcggcttg gagtgtactt ttgcgtgtcc
     1321 tgtttattat ttctctttag aagacgaagt tcctccgaaa agagaatctt ttgcggagat
     1381 gcctctggcg ccggggtttg attttgaatg tccatttgtt ctgttgaaag acgggcgcaa
     1441 gaagtcgaat cccgtaattg cggcaattat cagggatttt tgtcgctgat ttcgagcggt
     1501 tttctccggg aagcctcgca gaagcagctt ctttcttcaa tgcaaaaaca tcttggctat
     1561 atcataagat caaatgagaa gttcgactaa aagctgagga ggcgtatata gactcttaac
     1621 tattcttctg cttccgaaga aaaacgtgga ttcataagcg gaataacaat tggaggtgta
     1681 agtcctgcct gccctgtaaa tgatagaata gctgcgcgta gtactggcag aagtattgaa
     1741 gtaccgctct cagcaactat tttttctaat gccttttctt cgagagtttc ttggcattcg
     1801 aagataccgt ccatgcttgc ctttatcata agataatctt tctcatcggc tctaccaaat
     1861 gctgttagag tgagctcaag aagacagttc ttctcaccgt cttcttcgat gatttttgtc
     1921 tctctaccga gttcaataga tatcacgatc
//
LOCUS       DAOQYI010000144         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_69006_length_1946_cov_72.569540, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000144 DAOQYI010000000
VERSION     DAOQYI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69006_length_1946_cov_72.569540"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..508)
                     /locus_tag="PKL93_11545"
     CDS             complement(314..508)
                     /locus_tag="PKL93_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71935.1"
                     /translation="MIEIPGVLVLANQIHETLSDKKAKMVEVPIKSRKYAFHFAIFAM
                     REFGELCEYFLLLHENWVES"
     gene            complement(803..1480)
                     /locus_tag="PKL93_11550"
     CDS             complement(803..1480)
                     /locus_tag="PKL93_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNQ71936.1"
                     /translation="MGILDRFNDIIKSNVNALLDKMEDPSKMIDQHLRELSEDLAEVK
                     RGTAGVIAEETRTKRLVDENAAEVAKYADFAKRALLARNDGDAKVFIAKKQELENVGA
                     GLMTAYAAAHENAVKMRQMHDKLVSDIESLKNRRAMIKAKVAVAKTQKKINEVSSSAG
                     KTQGAIDAVGRLEEKAQKMLDEANAMAELNTEPTDKAKALEEKYAAKSSFSVDEELEQ
                     MKKELGI"
     gene            complement(1524..>1946)
                     /locus_tag="PKL93_11555"
     CDS             complement(1524..>1946)
                     /locus_tag="PKL93_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71937.1"
                     /translation="LIVFFEYGDGYMDRYVCGNQAKTVIDDEAADILLDYIDKYYYYD
                     NFADEEFFAKAFSDAADRIMTVTRSPWIPVFIVIGIIAVIVLLLFWWSNVKRQKNIEA
                     KQTQEILNTPLEKFGDQEVEELTKKYEKKDEGNESKES"
BASE COUNT          478 a          504 c          361 g          603 t
ORIGIN      
        1 ttttgcaaga gaagacttca agctgtgttt gccggctcct taacagccga gcatatcaag
       61 aaaaaagtaa gcctcgttga actgatcgcc aagcggcgaa ggcttttgct gagccgcaag
      121 atattacttg ttgttgaatt ctccctcact gaagcgccaa atgccaccgt aaagcttggt
      181 tttctcaata aaagatgttc cgtcactgaa tattattctc agctgcaatt gagttccggc
      241 gtttcgactt tatcgtgaaa ggcatttgcc gctccatcag acaagagtga tcctcttctt
      301 tcaaagcata tctctacgat tccacccagt tctcgtgaag aagtaaaaag tattcgcaca
      361 gttcaccgaa ttcccgcatt gcaaagatcg cgaaatgaaa ggcgtatttt ctagacttta
      421 tcggtacttc aaccatcttt gcctttttgt ccgaaagagt ttcgtggatc tgatttgcaa
      481 gaaccaatac ccccggtatc tcgatcactc ttaaccacta acacttactg tcagttatca
      541 cagaagatcc ttgcaaatca agaagtacca aattcttcct gtcgttcagt tgcttcttcc
      601 aaagactcga actgtcacaa aaagagaaag gccgaagaga atcactttaa tgcagcggga
      661 aagcgctgca tcaatcttcg accctactta caagttattt accaatttca gtataatgac
      721 gtacatgaac cgtcttcgaa tagaatcttc ttgagatact gttcctctga agaaagtaga
      781 gacaaaactg tctaaagagt ttctagatcc ccagctcctt cttcatctgt tcaagctcct
      841 catcgactga aaaggagctc ttcgccgcat atttctcttc gagagccttt gctttgtcag
      901 tcggctcagt attgagttcg gccatggcat ttgcttcatc aagcatcttc tgtgcctttt
      961 cttccagtct cccaaccgca tctattgctc cctgcgtctt acctgccgaa gaactaactt
     1021 cattaatctt cttctgagtt tttgcgacgg ccaccttagc tttgatcatt gcgcgtctgt
     1081 ttttgagaga ctcaatatcc gaaactagct tgtcgtgcat ctggcgcatt ttaacagcgt
     1141 tttcgtgggc tgccgcgtaa gccgtcatta gccctgcgcc cacgttttcg agctcctgct
     1201 tctttgcgat aaagaccttt gcgtctccgt cgtttcttgc cagcaatgcc cgctttgcaa
     1261 agtcagcgta ttttgccact tctgccgcat tttcatcgac cagtctcttc gttctcgttt
     1321 cctctgcaat tacacctgca gttcctcgct tcacttcggc aagatcctcg ctaagttcac
     1381 gaaggtgctg gtcgatcatc ttcgaaggat cttccatctt gtcgagcaaa gcatttacat
     1441 ttgacttgat aatgtcattg aacctgtcaa gaatacccat cttttcctcc cagaatcaag
     1501 cctctaaggc tcgtgtgata gcttcaactt tcttttgact catttccttc atctttcttc
     1561 tcatattttt tggttaattc ctcaacttcc tgatctccga acttttcaag aggggtgttc
     1621 aagatctctt gcgtctgctt cgcctcgata ttcttctgtc tcttcacatt gctccaccag
     1681 aaaagcaaga gcacaatcac tgctatgatt cctattacaa tgaataccgg aatccaggga
     1741 gatctggtta cagtcatgat cctgtctgcc gcgtcgctga atgccttggc gaagaactct
     1801 tcatcggcaa agttatcgta ataataatac ttgtctatat aatcgagaag gatatcggca
     1861 gcctcgtcgt cgataactgt cttcgcctga ttaccgcata catatctatc catataaccg
     1921 tcgccgtatt cgaaaaagac tatgag
//
LOCUS       DAOQYI010000145         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_69548_length_1937_cov_62.565356, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000145 DAOQYI010000000
VERSION     DAOQYI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69548_length_1937_cov_62.565356"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1035)
                     /locus_tag="PKL93_11560"
     CDS             complement(70..1035)
                     /locus_tag="PKL93_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNQ71938.1"
                     /translation="MRMSRETLSRLSGQTGYRQEIIEKVSLLLSWLDRASSIDRLKGS
                     FALKGGTAINLFFLEIPRLSVDIDINYIGSPTREELDRDRDGFENIIESVCHEESLSI
                     RNKRDSEAATSYSLRYVSSLSSGGNLSIDINYMMRVPLFEIETMDSRQLGSQIARRVP
                     LVNKLEVFASKIAACFSRETSRDLYDVYLLHKQIDTLDLEKLRLAFTVFGGLNRKNWL
                     EIDEKSLRVNKNDLKKRLLPLLRGTLDKEELDTLASDIERSCQKVASYLLPLKDNERE
                     FLRILNEKGEIRPEIITDDKELVDRIKKQPMLKWKALNVRQTLQG"
     gene            complement(1032..1835)
                     /locus_tag="PKL93_11565"
     CDS             complement(1032..1835)
                     /locus_tag="PKL93_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNQ71939.1"
                     /translation="MRLELFFEESPVFTTRDLKEFLKKNEAKSLENYSVLLNYHVKRG
                     RVLKICRGLYAVVPLNQKAEQFAPDSFLIAGKRTEDAILAYHTAQEFNGNTYSLFSTR
                     TFLTMKSVHPLVFREIEYVPTHPSQNLLRSDNYFLLTEQVDIKGINVLITSRERTFAD
                     MLDRPNLCGGIEEVWRSLKMTEYLNTENLLNYLRALDNSTTSAKVGFFLQRHPKITNN
                     DQTLLRELKKMIPVSPHYFDRSRVDRSRLVKDWNLVIPEYILNERWEEQ"
BASE COUNT          494 a          417 c          405 g          621 t
ORIGIN      
        1 atgttttatg aacatagagc actacgggat gacagtacaa gggcgttaca gttcacgaga
       61 cttctttgat cagccttgaa gagtctgcct aacattcagt gctttccatt tgagcatagg
      121 ctgcttcttg attctatcta ccaattcttt gtcatcggta atgatttctg gtctgatttc
      181 gcctttttcg tttaggattc ttaagaattc tctttcattg tctttcagag gcaacaaata
      241 ggaggctact ttctggcaag atctttcgat atcactggcg agagtgtcga gttcttcttt
      301 gtcaagtgta cctcttagaa gtggcaacaa tcgttttttc agatcattct tgtttactcg
      361 aagtgatttt tcgtctattt cgagccagtt ctttcgattc aaccccccaa aaaccgtaaa
      421 ggcaagccgg agtttttcaa gatcaagggt gtctatttgc ttatggagca aataaacatc
      481 ataaaggtct ctgcttgttt ctcgagaaaa acaggccgcg attttcgagg cgaaaacttc
      541 cagcttgttt actaatggaa ctctgcgggc tatttgacta cctagctgcc ttgaatccat
      601 tgtttctatt tcaaacagtg gaaccctcat catgtagttt atgtcaatag acaggtttcc
      661 tccagaagat agggagctta cgtacctcaa tgaataagag gtggccgctt ctgaatccct
      721 tttgtttctt atggacaggc tctcttcgtg gcaaactgat tctatgatgt tctcaaaacc
      781 gtctctgtca cggtcgagtt cttctcgagt gggtgatcca atatagttga tatctatatc
      841 taccgacaat ctcggaattt ccaggaagaa tagattaatt gccgttcccc cctttagagc
      901 gaaactccct ttaagacgat cgatacttga tgcgcgatca agccatgaaa gcagtagaga
      961 gactttctca ataatctcct gccgataacc cgtctgaccg gataatctgg acagagtctc
     1021 cctggacatt ctcattgctc ttcccacctt tcattcaata tgtattcagg aattacgaga
     1081 ttccaatcct tcaccaacct ggatctgtca acacgtgatc gatcaaagta atgcggcgaa
     1141 actgggatca tttttttaag ttctctcaat agagtctggt cattgtttgt gattttcgga
     1201 tgtctttgta agaagaagcc gactttggcg ctagtggtag agttgtcaag cgctctcaga
     1261 taattcagca ggttttcggt gtttagatac tcggtcattt tgagggaacg ccagacctct
     1321 tcaattccgc cacatagatt gggtctatcg agcatatctg caaatgttct ttctctactg
     1381 gttataagaa cgtttatacc ttttatatcc acttgctccg tgagtaggaa gtagttgtct
     1441 gaacgaagca ggttctgaga ggggtgagtc ggtacgtatt cgatttccct gaatactaaa
     1501 ggatgcacac tcttcatagt caagaatgtt ctggtactga aaagcgagta ggtattccca
     1561 ttgaattcct gagccgtatg ataggcaaga atcgcatcct ctgttctctt ccctgcgatg
     1621 agaaatgaat caggggcgaa ttgttctgcc ttctggttta ggggtaccac tgcgtagagg
     1681 ccacgacaga ttttgaggac acgacctctc ttaacgtggt aattcagaag aacgctgtag
     1741 ttctcaagac tcttcgcttc attcttcttc agaaattctt ttagatcacg agtcgtgaag
     1801 accggtgatt cttcaaaaaa aagttctaac ctcacttgat ctcctctccg tgacttgaca
     1861 tctagtggca tatagtataa atattttata tttttaggtg atccatgtca agcacaatga
     1921 taacgtcatc gtggtac
//
LOCUS       DAOQYI010000146         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_72490_length_1889_cov_72.319520, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000146 DAOQYI010000000
VERSION     DAOQYI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72490_length_1889_cov_72.319520"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            614..1459
                     /locus_tag="PKL93_11570"
     CDS             614..1459
                     /locus_tag="PKL93_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNQ71940.1"
                     /translation="MERSVVSPSENAESFVEIGFAKTNLTIVKEFVLGILGGAFIAFA
                     AQGSNVAIHTIGSVGLSKTLAGALFATGLMMVVVSGAELFTGNSLIVISCLQKKSKWV
                     SMFRNWLFVYLGNFAGSLLIAYFVLHSGQLGFSEGLLGGYTIKIAAYKTGLSFESAFF
                     MGILCNWLVCIAIWMASASKDIAGKILAIFFPIWLFITSGFEHSVANMYYIPAGILAK
                     ANEEFAKAAISLGVSAEQIANLNWGTLFTKNLIPVTLGNIVGGAFFVGTIYWFVYLKK
                     GKSAQ"
     gene            complement(1548..>1889)
                     /locus_tag="PKL93_11575"
     CDS             complement(1548..>1889)
                     /locus_tag="PKL93_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71941.1"
                     /translation="ENLFFVPERGSVLHALSKDGFMVLDEGPTVALYSAQRVFTLKRT
                     AILRIEMLKQVQMTPRKGFRLQDRGLVNRTGLPIQGFAANEVVIQTAVLGSPSNCHPE
                     LVSGSGRRPHY"
BASE COUNT          459 a          457 c          436 g          537 t
ORIGIN      
        1 ttctgttcac aagacccata ccctgtaatc gataccccgt tctcggcgtc atgaacttga
       61 ttcagaatct cgctctttcc agcgttcagc gtttcttagc aagcgggaag cggctcttct
      121 cgatgaacgg agaaccgctc aacggtcgac tagtttctct tgaactttcg aacgcccagc
      181 cacggtttct ccaaagccca gatcctgaac aggagcactt cactgacaaa atgagttcgt
      241 ctgatgagat tacttcaatt tgcttgctct ttttttggtt tttgcctcct ggtgtgtaag
      301 cgccagcatc gcttccccgg atgaccttcc gggcatcact tcctcgcgcc agagagccca
      361 cttcctgccg gaggcagcat cacttctgat cttgatgcca ccaacctccg accaccaacc
      421 gcggtcttgc aactgattct ttcctctgaa aagaagcgtc gatactgcct tcttgaatat
      481 gatccccact ctagttctta tcgctgctac tcttagtgtg tgataacatc taagtgtccc
      541 tcatcattgt cggcttttcg ggaagttccg tatgaatggt ttccagatgt atagataaca
      601 gggggttttt gaaatggaaa ggtctgttgt aagtccttcg gaaaatgctg agagttttgt
      661 cgagataggt tttgcgaaaa ccaatttgac tatcgtgaag gaattcgtgc tgggtattct
      721 gggcggtgca ttcattgcat ttgcggcgca ggggtccaac gtggcgatac acactatcgg
      781 ttcagtggga ctttccaaga ccctggcagg cgcattattt gcgacgggcc ttatgatggt
      841 agtcgtttcc ggagcagagc ttttcacggg taacagtctt attgtcattt cctgtttgca
      901 aaagaagtcg aaatgggttt ctatgttcag gaactggctc ttcgtttact taggaaactt
      961 tgcgggttcc ttacttatag cttacttcgt tctccattca ggccagctcg gcttttccga
     1021 aggtctgctg ggtggatata caatcaagat tgcggcttat aagacgggtc ttagtttcga
     1081 aagcgcattc tttatgggga ttttgtgcaa ctggctcgtc tgcatagcca tatggatggc
     1141 atccgcatcc aaagacatag ccggcaagat tctcgccatc ttcttcccga tatggctttt
     1201 cattacctca ggctttgaac attccgtggc aaacatgtat tatattcccg caggaattct
     1261 cgcaaaggca aatgaagaat ttgccaaggc cgcgatttct cttggagtca gtgctgagca
     1321 gatcgccaat ctaaactggg gaactctctt cacgaagaat ctaataccgg tgactcttgg
     1381 aaacatcgta ggaggcgctt tctttgtcgg tacaatctac tggtttgtct acttgaagaa
     1441 aggaaagtcg gcacaataat ggtgatcccg gactgtaagc ttcaaagaaa gcgtaagtat
     1501 aaatttgatt tgttggaaaa gcgataagaa agggcagcgt acttaattta atagtgaggt
     1561 cgacgcccag atcccgaaac aagttcggga tgacaatttg agggacttcc caaaaccgct
     1621 gtctggataa ctacctcatt tgcagcaaaa ccttgaattg gtaagccagt tctgttcaca
     1681 aggccccgat cctgtaatcg aaaccccttt ctcggcgtca tctgaacttg tttcagcatc
     1741 tctatcctaa gaatcgctgt acgcttaaga gtaaaaaccc gctgagcgct gtaaagagca
     1801 accgttggcc cttcgtccaa aaccatgaac ccgtccttcg aaagcgcatg aagaacagat
     1861 cctctctctg gaacgaagaa caggttttc
//
LOCUS       DAOQYI010000147         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_73054_length_1880_cov_61.108493, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000147 DAOQYI010000000
VERSION     DAOQYI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73054_length_1880_cov_61.108493"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PKL93_11580"
     CDS             complement(<1..501)
                     /locus_tag="PKL93_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNQ71942.1"
                     /translation="MKVVSSKYLDDRHDLLRNLVSILERDFPYVSILGTDVKGKLFRV
                     TTTGISVNDSRWSERGFVLRVHDGTGYFEFSFNEIDSESLNEIVELVKKRVTVFREAM
                     VKNNLEEAKTPIPEEKEWKLSFKDEVKILPGMLSAEEIMGRLSNLKDKAHSLSEEVIN
                     VMTLMEN"
     gene            761..1510
                     /locus_tag="PKL93_11585"
     CDS             761..1510
                     /locus_tag="PKL93_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ71943.1"
                     /translation="MVKVERITKTFENRRKRVIAVDSLSFNAEPGSIYGLLGPNGAGK
                     TTTLRIISTLLKPDSGRATVMGFDSVDAPTEVRRRIGFLTSDMKLSGNLTPRELLQFY
                     GELSHMRESEISERTKFLSDYLNMNDFLDKRIGKLSSGMKQKAAIAVSLIHDPEVIVF
                     DEPTNGLDIITARTVTEFLKDYRRKGKTIIISTHIMSVAEKLCNKVGIIFKGKLVADD
                     TLEGLYRRYDESSLEDVFFKIADLEGVTESV"
     gene            1503..>1880
                     /locus_tag="PKL93_11590"
     CDS             1503..>1880
                     /locus_tag="PKL93_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ71944.1"
                     /translation="MFRDALIIFRKELKNVFKDPRTVFAVLLLPMLIMPVIFLVMNVV
                     SQSQAKTYENTVYSLKVTNLPDERFLKILEGLISFEIVDELNEETVRDFENSIVVEFP
                     PNSAETIENGERIDALVFYNSTSR"
BASE COUNT          579 a          406 c          376 g          519 t
ORIGIN      
        1 gttctccatt aaagtcatta cgtttattac ttcctcagat aatgaatgag ccttatcttt
       61 taggttggat aatctgccca tgatctcttc ggccgacagc atacccggca ggatcttgac
      121 ctcgtctttg aaactcagct tccattcctt ctcctctgga atcggtgtct ttgcctcctc
      181 aagattgttc ttaaccatcg cctctctgaa aacggtgact ctcttcttca caagctcaac
      241 aatttcatta agagattcac tgtctatttc attgaaagaa aactcaaaat agcctgttcc
      301 gtcatgaact cgcagaacaa agcccctctc tgaccatctg gagtcgttga cgcttatccc
      361 agtagtagtt acccggaata actttccctt cacatcggta cccagaatag aaacgtaggg
      421 gaaatctctc tccaaaattg aaactagatt ccgcaacaag tcatgtctgt cgtctagata
      481 ctttgaactg actaccttca tttagaacct cccattatca aagggttact tttcatacta
      541 ataactccta gagcatcgca cggaagctaa tcgataccct tgacttcaaa tattatacta
      601 taattcatct taaaatgaaa taaatgaatc taacactcaa taaaattgat aattattcct
      661 tcaattatga aataattttt ctgatatctt caccactaaa cttcaatgta acataatggt
      721 gttattcttc actaagatag ataactggag gttagtagaa atggtaaagg ttgagagaat
      781 cacgaagacc ttcgagaatc gaagaaaaag agtgattgct gtagattcac tcagttttaa
      841 tgcagaacca ggtagcatat acggtctact gggtcccaat ggagcgggaa agaccacaac
      901 actaagaatc atctccactc tcctaaaacc cgatagcgga agagcaaccg ttatggggtt
      961 tgattctgta gatgccccca cggaagtgag gaggagaatt ggattcctga caagcgacat
     1021 gaagctatcg ggtaatctca ccccaagaga gcttcttcaa ttctatggag aactaagcca
     1081 tatgcgagaa tctgaaattt ctgagcggac gaaatttctt tctgactatc tgaacatgaa
     1141 tgattttctc gacaaaagaa tcgggaagtt atcatcggga atgaagcaaa aggctgctat
     1201 tgcagtcagc ctgatccatg atcctgaggt aatcgtgttt gatgagccaa ccaatgggct
     1261 agatattatt acggcaagaa ccgtgaccga atttcttaag gattatcgca ggaagggaaa
     1321 gacaataata atctccacac atatcatgtc cgtggccgag aagctttgca ataaagtggg
     1381 gataatcttc aaaggcaaac tggtcgccga tgacactctc gaaggacttt acagaagata
     1441 cgatgagtcc agtctggagg atgtcttctt caagatcgcc gatttggaag gggtgactga
     1501 gagtgtttag agacgcccta ataatcttcc ggaaagaatt aaagaatgta ttcaaagatc
     1561 cgagaacagt attcgcggta ctattgctgc cgatgttgat tatgcctgtc atcttcctgg
     1621 ttatgaacgt cgtatctcaa tcacaggcaa aaacttatga aaacaccgtt tacagtctga
     1681 aggtcaccaa ccttcccgac gaaaggttct tgaagattct cgaggggctg atctccttcg
     1741 agatcgtcga tgaacttaac gaagagacgg taagggactt cgaaaactcg atagtagttg
     1801 agtttcctcc aaattcagct gagacaattg aaaacggaga aagaattgat gccctcgtct
     1861 tttacaactc tacttcgaga
//
LOCUS       DAOQYI010000148         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_73257_length_1877_cov_79.097695, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000148 DAOQYI010000000
VERSION     DAOQYI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73257_length_1877_cov_79.097695"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1355
                     /locus_tag="PKL93_11595"
     CDS             27..1355
                     /locus_tag="PKL93_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ71945.1"
                     /translation="MTDQQMFSVLARWNYWEDRSLPELVYRREIGEIEEFLDSNFVQI
                     VKGPRRSGKSSLLKLVYRKLLESVDKLSFLFLNLEDVSLSMEEKTPVLLEKLYRLYRQ
                     RVNPLGKTYMFVDEIQHIRGWEQWIETYRESGEVKFIVTGSSSKLSSKELGTLLTGRH
                     IDLTLLPFSFADFVRSKGIEVSSLSIELKSDLIKNLLFSYLDSGGFPEILLNFPEENG
                     TKILEAYFDDIIYRDIVERWNVRDALLTKRIALYALKNSGNLLSYRKIQKALEQTSGK
                     TSTSTISENMSHLSEAYILFEVELHTNSVKKALINPRKVYSVDTGLRKAVVKPLTEDY
                     GRDGENIVFLELLRRGYKVTFWKTDKTEVDFVASGRTGDLVINVTMSNLDDPDLKERE
                     LRGLLEFEGDRCRRILLNSDIEDTLKIQDKTIELIPLWKWLIIEDFLPSR"
BASE COUNT          520 a          397 c          446 g          514 t
ORIGIN      
        1 aggtcaattt ttgataggtg gttgatatga cagaccaaca gatgttttca gtcctcgcaa
       61 gatggaatta ctgggaagat cgttctttgc cggaacttgt ttacagaaga gagatcggtg
      121 agattgaaga atttctcgac agtaatttcg tgcagattgt aaaaggacct agaaggtcag
      181 gcaaatcatc tcttcttaag cttgtgtaca gaaagctcct agaatctgtt gataaactct
      241 cctttctgtt cttgaacctt gaagatgtct ctctctctat ggaagagaaa acaccggtcc
      301 ttcttgaaaa actctacagg ctttacaggc agagagtcaa tccccttgga aagacctaca
      361 tgtttgtcga tgagatccag catatacgag gctgggagca atggatagag acatacaggg
      421 agtcaggaga ggtcaaattt attgttaccg gctcatcttc aaagctctct tccaaagagc
      481 ttggaacgct gttgacagga aggcacatag atctgactct gttgcccttt tcattcgcgg
      541 actttgtgcg atcgaaggga attgaagtct cctcactttc aattgaactc aaaagtgatc
      601 tcatcaagaa tctgctcttc agttatttgg attccggagg ctttcctgag attctcttga
      661 acttcccgga agaaaatggg acaaagattc tcgaggcgta tttcgacgac ataatatata
      721 gggacatcgt agagcgctgg aatgtcaggg acgctctgct tacaaagaga atcgcgctct
      781 atgctctgaa gaacagcgga aatctcttga gctacagaaa aatacagaag gctctcgaac
      841 agacctcggg aaagacttca accagcacaa tttcggagaa catgtcgcac ctatcggaag
      901 catacatatt gtttgaagta gagcttcata cgaattccgt caagaaggcc ctaatcaacc
      961 caagaaaagt atattccgtc gacactggcc tgcgaaaggc cgttgtcaag ccgctcactg
     1021 aggactatgg aagagatgga gagaacattg tctttctaga gctcttgagg cggggctaca
     1081 aagtgacttt ctggaagact gacaaaaccg aagttgattt cgtcgcctct ggaagaactg
     1141 gcgatctggt catcaatgtc acaatgtcaa atctggatga tcccgatttg aaagaacgtg
     1201 aactccgggg attactcgaa ttcgagggcg acagatgccg caggatactt ctcaacagcg
     1261 acattgaaga cacgttgaag attcaagaca agaccattga gctaatccct ctctggaaat
     1321 ggcttattat tgaggatttc ctcccaagca gatgatctac actatttctg acaggttttc
     1381 gtaagtgcaa agggcttaat ctgaactcag cgatctgctg ggttttcaga cggattcatt
     1441 tagaaagatg ccttctgtcc tctcaagcgc tctcgatcat atctatggac tggtctatgc
     1501 tttgggacga aggtacgaat caaggcccta tttcaccata acggtaagtg cagttgtgga
     1561 atgattttga tctccacacc tctattctct ccgcactggc ctcttaagtg atctttgatt
     1621 tccatttatc ggaaagatat cggcatttta tcggaaatga gagttgaggt tgattcgcga
     1681 aaaatgttca tacaggaatc attgggaggg atgggttagt tttgaagcga tagggtgtgt
     1741 gctctttgaa actgttctgc taactcatct gcggtaagcg gcaatgtgtc tttcaatctg
     1801 cagctgccct cctcgaacgg cggttttcac aacgttttac gaccaaagag gagcgggtgt
     1861 ttttcctctc gccagcg
//
LOCUS       DAOQYI010000149         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_83477_length_1734_cov_72.707564, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000149 DAOQYI010000000
VERSION     DAOQYI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83477_length_1734_cov_72.707564"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PKL93_11600"
     CDS             complement(<1..1286)
                     /locus_tag="PKL93_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNQ71946.1"
                     /translation="MSDRYLTIEEQLSLPIVNNPVISPDGKKLAYILKRADWNTNKYL
                     QTVVVYDAAIKTTLHVSNDEFDCSLPDWSPDSSKLAFLQKSGGENKNDVMIFDFEEKR
                     TFKLLTFENDISQIKWSRKNDGLYLVAASPESDELKKRKENYGEFEYIDEEPKNSSLF
                     FIHLSRAMKKCFSRFELPKDMRSEETLFDKLTDEKEFHVSSISLSPDGNYVALMAPPT
                     SDVNDFDEAKVMLMDSERKLKELPFKHPRGFAFSPDSKELAVVVPDGEDPWMDNGAIE
                     IVDLFNLSKERVVVEDDLTVELVSWTERGFFVSWIENSTISLGLLDMNGHLERLTDRD
                     ELVLTSSVTLDGKHIASAMGTNAEPLEIYLDGKKITEIAVSYPERKRVNKERISWKSY
                     DGTEIHGVLNLPEDFDSSRRYPLVVLVHGGPTWTALA"
BASE COUNT          412 a          427 c          386 g          509 t
ORIGIN      
        1 gctagggccg tccatgtcgg accgccgtgt acaagcacga cgagcgggta tctccgggag
       61 ctgtcgaaat cttctggaag attgagaacg ccgtgtatct ccgtaccgtc gtagcttttc
      121 caggagatcc gttccttatt cactcttttc ctctccgggt aggaaacggc gatctccgtt
      181 atcttctttc catctaaata gatttcaaga ggctccgcat tggtccccat cgcagacgct
      241 atatgtttac catcaagggt aacgctggag gtcagaacca actcgtcccg atctgtcaga
      301 cgttcgagat ggccattcat gtccagaagg ccaaggctaa ttgtgctgtt ctcgatccag
      361 ctgacgaaga agcccctctc tgtccaggag accagttcaa cggtcagatc gtcttcgacg
      421 acgacccgtt cttttgagag attgaagaga tctacaatct cgatagcacc gttatccatc
      481 caggggtctt ctccatctgg taccacgacg gcgagttcct tcgaatcagg agagaaggcg
      541 aagcctctcg gatgcttaaa gggtagttct ttcagttttc tttcgctgtc cataagcatg
      601 accttggctt catcgaaatc gttgacgtcg cttgttggcg gagccatgag tgccacgtaa
      661 ttaccgtccg gagaaagaga gatcgaagaa acatggaatt ccttctcatc tgtcagttta
      721 tcgaagagag tctcttcaga tctcatatcc ttgggcagtt cgaaccttga aaagcacttc
      781 ttcatcgcgc gtgagagatg aatgaagaaa agagagctgt ttttcggttc ctcgtctatg
      841 tattcgaatt cgccgtagtt ctccttcctc tttttcagtt catcgctttc aggagaggcg
      901 gcaaccaggt aaagcccgtc gttctttctg ctccacttta tctgacttat gtcgttttcg
      961 aaagtcagca gcttgaacgt tcgcttctct tcgaaatcga agatcatgac gtcgttcttg
     1021 ttttcgccgc cgcttttctg gagaaatgca agtttactcg aatcgggaga ccagtcggga
     1081 agagagcagt cgaattcatc gttcgagaca tgtaaagtgg tcttaattgc tgcatcataa
     1141 accacaactg tttgcaaata cttgtttgtg ttccagtcgg cgcgcttcag tatataggca
     1201 agtttctttc catcgggcga aattaccgga ttgtttacga tcggaagcga aagctgctct
     1261 tcaatcgtga gatatctgtc gctcaaatca tacccctccc atctaagata agaaaactat
     1321 actatcatag tctatccttt gaaaacaaac aaaatacctg ccaacagcct tctctccgaa
     1381 tcaaagtcta tttcaaacgc ttgccatgac cttactttaa gacacaggat tctagctcac
     1441 agatatataa tcttgaagaa gatacgtagg agaggttttc tctatcgttc atatttgcta
     1501 tttaatccat tctcttggca ggattcattc gggtttcggg taaaaaatgt caatcccggc
     1561 tgaatgaact aaaggagatg ttggcatgaa gattaccttc cttcctcgct ctcgattagg
     1621 caagtggggc ttctctctga gtctggcctt catccttttg ctctttgccc aggtacactt
     1681 ctcattgatt cctctgccag tcttctctgt tatggctcta ggagtggccg gctt
//
LOCUS       DAOQYI010000150         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_86325_length_1698_cov_69.642727, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000150 DAOQYI010000000
VERSION     DAOQYI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86325_length_1698_cov_69.642727"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1084)
                     /locus_tag="PKL93_11605"
     CDS             complement(5..1084)
                     /locus_tag="PKL93_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HNQ71947.1"
                     /translation="MKRLPMCALFFVLLTVASIGCTGFRVISKDGAIVAARTLEFEFD
                     LGSKIVVAPRDTFMTALTTGWEGLSWNVKHGYVGANALGMDLVADGINEAGLACGGFH
                     FTGCAGHQPLEKDLYKSTISHYDVIGLILENFATVEEVKSALDDIRVYGLAIMITEST
                     MMPLHYIVYDAHGGCIVIEYVDGELNIHENPLGVITNNPTFDWHLQNLSNYVNLTPAN
                     IKSSKLGDFEIRATGNGTGLLGLPGDNTSPSRFVRAAFYSHGEVKPASAESAVTLAWH
                     ILNAQDIPEGLVVNEELGIVISRDIAYWSAVRDLTNRVYYYRTYEDLNIRKVELDRID
                     FSSGNIRFLNMSHEQNYIDVTDAIK"
     gene            1283..>1698
                     /locus_tag="PKL93_11610"
     CDS             1283..>1698
                     /locus_tag="PKL93_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ71948.1"
                     /translation="MLDEAIKVKNLRKTYGNFEAVKGINLSLVPGKVNALLGPNGAGK
                     TTTLKSILGLVHYSGEITILGETIDVARERISFVPEDKSFYDSLTPEKAIKMCSMLMS
                     NFSAEKAAKQMKDFDLPMNKKISAFSHGMKTSTYLA"
BASE COUNT          475 a          409 c          351 g          463 t
ORIGIN      
        1 tttttcactt gattgcgtca gtcacatcta tgtaattctg ttcgtggctc atattcagaa
       61 atcttatgtt cccgcttgag aaatcgattc tatcaagctc tacttttctt atgttcaagt
      121 cctcgtaagt tctgtaatag tagactctgt tggtcaaatc ccttactgcc gaccagtaag
      181 ctatgtctct tgaaattact attccgagct cttcattgac tacgaggcct tcaggaatat
      241 cctgagcgtt gaggatatgc catgctagtg tgacggccga ttcagcacta gccggtttca
      301 cttctccgtg agaatagaag gccgccctca caaatcggct tggagatgtg ttgtccccag
      361 gaagcccaag caatccagtt ccatttcctg tcgccctaat ttcaaaatca cctaactttg
      421 aactcttgat gtttgcggga gtcaggttta cgtagttcga gagattctga agatgccagt
      481 cgaatgtggg attgtttgta attactccaa gcggattttc atgaatgttc agttcaccat
      541 caacgtattc tattacaatg catccgccat gagcatcata gacaatatag tgaaggggca
      601 tcatagtaga ctccgttatc ataatggcaa gcccgtacac ccggatatca tctagcgccg
      661 attttacttc ttccaccgtt gcgaagtttt caaggatcaa gcctatcaca tcataatgag
      721 atatggttga cttgtagagg tctttttcga gcggctgatg cccggcacag cccgtaaagt
      781 ggaatcctcc gcacgcgaga ccggcttcgt ttattccatc tgcaaccaga tccataccca
      841 aagcgttggc cccaacgtaa ccgtgcttca cgttccagct caggccctcc caacccgtcg
      901 taagtgcagt cataaaagta tctctgggtg cgacgacaat cttgcttcca agatcgaatt
      961 cgaactcgag agtccttgca gcaacaatag caccatcttt cgaaatcact ctgaacccgg
     1021 tgcacccaat cgaagctaca gtcagcaaga cgaagaaaag agcgcacatt ggaagtctct
     1081 tcattgcaga catccccttt ctgtgttttg atggccttat ctctcgcttc tctaaagaca
     1141 gcaatatata cattaggggc actttctgac ttctatattg aagaatgatt ggaaaaaaca
     1201 aaaaattcag cgcaccccca ttgacaacca actgttctag ttgatatagt acagttaaga
     1261 tagtcagcga ggagtgaatt ctatgttaga cgaagcgatc aaagttaaga acctcagaaa
     1321 gacctacgga aatttcgagg ctgtaaaagg gataaatctc tcccttgtac ccggaaaagt
     1381 caacgctctc ttgggaccta acggtgccgg aaagaccaca actctaaaat caattcttgg
     1441 ccttgttcat tattcaggag agataacgat ccttggagag acaatcgatg tcgcccggga
     1501 gagaatatca ttcgtcccag aagacaaaag cttctatgac agcctgactc cggaaaaggc
     1561 aattaagatg tgttcaatgc tcatgagcaa tttctccgca gaaaaggctg ctaaacagat
     1621 gaaggacttt gatctaccga tgaataagaa gatatctgct ttctctcacg gcatgaagac
     1681 atccacttat ctggcaat
//
LOCUS       DAOQYI010000151         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_86723_length_1693_cov_72.095238, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000151 DAOQYI010000000
VERSION     DAOQYI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86723_length_1693_cov_72.095238"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..84)
                     /locus_tag="PKL93_11615"
     tRNA            complement(8..84)
                     /locus_tag="PKL93_11615"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Met,seq:cat)
     gene            complement(91..167)
                     /locus_tag="PKL93_11620"
     tRNA            complement(91..167)
                     /locus_tag="PKL93_11620"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(131..133),aa:Met,seq:cat)
     gene            complement(227..315)
                     /locus_tag="PKL93_11625"
     tRNA            complement(227..315)
                     /locus_tag="PKL93_11625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:Leu,seq:taa)
     gene            complement(362..>1693)
                     /locus_tag="PKL93_11630"
     CDS             complement(362..>1693)
                     /locus_tag="PKL93_11630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ71949.1"
                     /translation="MTLTPLGGGREIGANSYLLSLDGKNILIDAGRHPVKEGYESLPE
                     IDKINDLDVIMISHSHYDHLSSLPYISQRWPNAPILMAKENKGLALRILQNSVEVMKK
                     KNAKDGVEPILYNHGEVNELKRRIYPMEYFQRYSIDDVVDVALYPAGHVMGAASIFLE
                     HRGRRILYSGDISLSQQLTIPPAQLPKKVETLISEGTYGAKENQLLTRYSEIERLSKS
                     VKRVLENGGRILMPVFALGRGQEAIYMLLMLMEKKKIPSVPIYTNGMVASLSNLYMSN
                     YESISEKDRKWFIKSFQNNVTVAPKNIERLLFSREPALFVFSAGMLVEDTLSYIFAKQ
                     MLPNSRDGIFFMGYQSPESDGYKILQANKNGDERIQMGDDSIEIGTKNIDIFNFSGHA
                     DYQELLDLPRRLQPEKLIYVHGDEAALENLAEELQYEFEIQIPSNLQTVEL"
BASE COUNT          373 a          424 c          415 g          481 t
ORIGIN      
        1 aaatgcttgg tggcggcggt cagaatcgaa ctgacgactc tgcgggtatg aaccgcatgc
       61 tctagccagc tgagctacac cgcctaataa tggtagcggg gacaggattt gaacctgtga
      121 cctttgggtt atgagcccaa cgagctacca gactgctcca ccccgcaccg agtatctatc
      181 tgctcacaat attctatata ttaatgacct gctttcttcg cttccatggt gccggggatg
      241 ggaatcgaac ccacacggag ccctcgctcc aacggatttt aagtccgttg cgtctgccag
      301 ttccgccacc ccggcaaccg tacactatga taacaccggc acatttcctt gtcaaccgga
      361 gctagagctc gactgtctga agattcgatg gtatctgaat ctcaaactcg tactggagtt
      421 cttcggcaag gttttccagc gcggcttcat caccgtggac gtagatcagt ttctccggct
      481 gaagtcttct gggaagatcc agcagctcct ggtagtctgc gtgaccggag aagttgaaga
      541 tatctatgtt ctttgtgccg atctcgatcg aatcgtctcc catttgaatt cgctcatcgc
      601 cgttcttatt tgcctgtagg attttgtaac cgtcggattc gggactctgg taacccatga
      661 agaagatccc gtctctgctg ttcggcagca tctgttttgc gaatatatac gatagtgtat
      721 cctcgacgag catcccggca gagaagacaa atagagcggg ctctctcgag aagaggagcc
      781 tctcgatgtt ctttggagcc acagtaacgt tattctgaaa gctcttgatg aaccactttc
      841 tgtccttttc gcttatactc tcgtagttgc tcatgtaaag attgctgagg gaggccacca
      901 tcccattcgt gtatatcggg acggagggga tctttttctt ttccatgagc atgaggagca
      961 tatagattgc ttcctggccc ctaccaagag cgaacacagg catgagaata cgaccaccgt
     1021 tttccaaaac gcgcttcacg gatttgctca gacgctctat ttcgctatat cttgttagaa
     1081 gctgattttc tttcgcaccg tatgttccct cggatatgag tgtctcaacc tttttgggaa
     1141 gctgggcagg tgggatggtg agctgctgcg aaagcgaaat atctcctgaa tacagtatcc
     1201 gccttcctct gtgttcaaga aagatcgagg cggcgcccat aacgtggcct gcagggtaca
     1261 gggcaacatc taccacatcg tctattgagt atctctgaaa gtactccatg ggatatattc
     1321 ttctcttcag ctcgttcact tcgccgtggt tgtagagtat cggctcgacc ccgtccttgg
     1381 cgtttttctt cttcatgact tcgactgagt tctggagtat tcttagcgcc agacctttgt
     1441 tttcctttgc catgaggatc ggggcatttg gccatctttg agaaatataa ggtagagaag
     1501 agagatggtc gtagtgggag tgagagatca ttatcacatc tagatcgttt atcttgtcaa
     1561 tttccggtag cgactcgtaa ccttccttta ctgggtgtct tcctgcatca attaggatat
     1621 tcttgccgtc cagactaagg agataggagt ttgcgcctat ctcgcggccg ccacctagcg
     1681 gcgtaagtgt cat
//
LOCUS       DAOQYI010000152         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_89749_length_1658_cov_63.949470, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000152 DAOQYI010000000
VERSION     DAOQYI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89749_length_1658_cov_63.949470"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKL93_11635"
     CDS             complement(<1..307)
                     /locus_tag="PKL93_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71950.1"
                     /translation="MRFVRVFGTTEGYLSRFNEDYRIDGFSRSLRGLSKSDRVLRSFL
                     DILLEYAELRNAIVQTVRADTRPIAELHDEVVQDIERIARMLNDPPRITSVFGYEKVA
                     "
     gene            complement(828..1100)
                     /locus_tag="PKL93_11640"
     CDS             complement(828..1100)
                     /locus_tag="PKL93_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71951.1"
                     /translation="MIALREKEKEIVKRQIKELVSEYAEKVLCSKAEAILDSNLLKDE
                     EIEDLIQDGRAYTMAIALINDWLLSLPYPLEGKSYKKLRKLLEKLR"
BASE COUNT          444 a          356 c          351 g          507 t
ORIGIN      
        1 tcgctacttt ctcgtatcca aatacagaag taatccttgg cggatcattg agcatccgag
       61 cgatccgctc tatgtcttga actacttcgt cgtgtagctc tgcgataggt ctggtatcgg
      121 ctcttactgt ctggactatc gcgttgcgta actctgcata ttccagaaga atgtcgagaa
      181 agctcctgag aaccctgtca ctcttcgaca aacctctcaa agatctggag aatccatcga
      241 tccggtaatc ttcgttgaat ctgctcaaat acccttctgt cgttccaaag acacgcacga
      301 atctcactca attctccatg gtcaatccgc ctgactaaag aacacttatg ctgagaaaaa
      361 aatgaactac ggtgagaaac agaaatggat tctcacttgt gtcttcctct taattattaa
      421 agtataacaa aacggtggtt tgggagaaaa cgggtttcag ctcatagtcc gtggttagag
      481 tagattggtg ggtttctcta tcgaagagca ttaaattgtt ttacgtatga gtatgagcag
      541 gcctgttcgt aattttagcc tattcctgat tatcaagaag tctctttctt ctggttgttc
      601 agctcttaca attatgcatc caataattca aagagcagcc gggggaaggg ctgctctttg
      661 aagaaactaa gctacactgc ttttcttctg accttggtcg tgttgataga gcaagctgca
      721 ttttgttgga cgaatcatgt tgaacagtta cagagttgcc tatttcatcg gattttgttg
      781 caatgtgatt tttctcgctc agagaacgga aaattgaagt ccgggttcta tctgagtttt
      841 tctaatagct ttcttagctt cttgtatgac ttgccttcga gaggatatgg cagggaaagc
      901 aaccagtcgt taatcagcgc aatagccatt gtatatgccc tgccatcttg aatcagatcc
      961 tcaatttcct cgtccttgag caggttgctg tctagtatag cttctgcctt gctgcataga
     1021 actttctcag cgtactctga cacaagttcc ttgatctgcc ttttcactat ctctttctct
     1081 ttctctctca aagcgatcac ctttgcatcc tcaagaacta agtcaacggc caagcgttgt
     1141 tgacaaaaag aaccatgcaa aacaatgcct acgtggcatt tatccaagtt cataggtaag
     1201 ctcggctgtt tacgcgcagt tctttgcaga aaacaagatt gtgctcaaag tttttgtagc
     1261 aaatcagggg atttcaagac aataaaagat gaagaagtcc ctggctttct gatgatttat
     1321 tgcttcattt cttccagttt gggtggcaaa tgattctcgc ttctctacac gataatcgag
     1381 acccgagggt gctttcctga tgagctaagg aggacgttca aagtgaaagg attattccac
     1441 aagatctaat cgaaagaatg attactcttg ctgaactttt ctgaaatgag gactcaataa
     1501 ctcccttcga atcaaatttg gtagcgaact agaacgcgtg acttgctaat gacagggaag
     1561 acggcaatgt gagcattaag attcgaggct gcggaaatta gtttccttaa tgcgactgat
     1621 ttgttgaaat ccacttctaa gagaagcggc cgattgac
//
LOCUS       DAOQYI010000153         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_96263_length_1589_cov_100.767275, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000153 DAOQYI010000000
VERSION     DAOQYI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96263_length_1589_cov_100.767275"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1589)
                     /locus_tag="PKL93_11645"
     CDS             complement(<1..>1589)
                     /locus_tag="PKL93_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71952.1"
                     /translation="EWYLDNAAIGGKKKGLQVRIAPFTGVNVEGLEFESMNHRDTVYV
                     IPEADDTIGDLAREIKSLDASLNSFRTKKTGEGIHEILTKYGRIMNDKKNELGRVLRK
                     AIDSGQLLYEGELKPTSSLINELKDLLKEKVIPGHYTEITYTTATSKDTDVVLSGPQN
                     TLKTIRLDDDHRVFNDNGELIETHKIISPVIEYLGEDQTGETLLEKFSSPPYGWTPET
                     IIYSVACLIRGGKIMVNNSDYYGKADVHKALKSVSEFKKARIRRSVVLKPSDKQYLMD
                     IINPLLDDGRLSLQSPRSEFISRALEAMKHLDKRMNELKEKMEKLGADVKWNLDTLRT
                     MINTLTGGDSDCLDDLLRERDSIRELKDTADKTEQFLDKNYELIRRQKTFLHELEGEI
                     SKGAFEKDQSEKLSAILKEYKDTLPSIASFGTDLDSTFENLRNTYKSYFNPIHDERDE
                     WLKKIHEYLDSIQDERNSLGKRAGDQNWFRRPTPPCGELEIQFSIKCEKCHTGLNEAS
                     LYITEFSNRLEKLKDSFDSFM"
BASE COUNT          301 a          403 c          384 g          501 t
ORIGIN      
        1 cataaagctg tcgaaggagt ccttaagctt ctccagcctg ttagagaact ccgttatgta
       61 aagactggct tcattcaatc cggtgtggca cttctcgcat tttatggaga actgtatctc
      121 aagctctccg cagggaggag ttggtcttct gaaccagttt tgatctcccg ctctctttcc
      181 cagagagttt ctctcatcct gtatggaatc gagatattcg tgtatcttct ttagccattc
      241 gtctctctcg tcgtgaatgg ggttgaagta gctcttgtag gtgttgcgta gattctcgaa
      301 ggtgctatcg agatcggtgc cgaaggaggc tatgctgggc agagtgtcct tatactcttt
      361 caagatcgcg gaaagttttt cactctggtc tttctcgaag gcgcccttgg agatttcacc
      421 ttccagctcg tggaggaagg ttttctgtct tcgaatcagc tcgtagttct tatcgaggaa
      481 ctgctcagtt ttgtcggcag tatccttcag ttcgcgtata gaatctctct cgcgaagcag
      541 gtcatccaga cagtcgctat cgccaccggt gagagtgttt atcatcgttc ttagagtatc
      601 tagattccat ttcacatcgg caccgagttt ttccatcttt tctttcaact cattcattct
      661 cttgtcgaga tgtttcatcg cttccagtgc ccgagatata aattcgctcc tgggtgactg
      721 gagcgacagt ctcccgtcgt ccagaagcgg gtttatgatg tccataagat actgcttgtc
      781 ggacggctta aggactacgc tgcgccgtat cctggccttc ttgaattcgc tcacgctctt
      841 cagtgcttta tgcacatcgg cctttccgta atagtctgaa ttgttcacca ttatctttcc
      901 gccacggatg agacaggcca ctgaatagat tatcgtttcc ggggtccagc catagggagg
      961 agaagagaac ttttccagca gagtctctcc cgtctgatcc tctcccagat actcaatgac
     1021 cggagagatt atcttgtgtg tctcgatcag ctccccgttg tcgttgaata ctctgtggtc
     1081 gtcatctagc cggattgtct tcagcgtatt ttgaggtccg ctcaatacta cgtctgtgtc
     1141 cttgctcgta gcggttgtgt aggtgatttc tgtgtaatgg ccggggatta ccttttcctt
     1201 gaggaggtcc ttaagctcgt ttatcaggct ggatgtaggt ttcaattcgc cttcgtaaag
     1261 aagctgtccc gagtctattg cctttcgcaa cacccgaccc agctcgtttt tcttgtcgtt
     1321 cattatcctg ccgtacttgg tcaggatttc gtgtatgccc tctcccgtct ttttcgttct
     1381 gaaggagttc agcgaggcgt ccaggctctt tatctccctt gccagatcgc caatggtgtc
     1441 gtcggcctcg gggattacat agacagtatc gcggtggttc attgactcga attcgagacc
     1501 ttccacgttc acaccggtga agggagcgat gcggacctgg agacccttct tcttgccgcc
     1561 gatcgctgcg ttgtccaggt accattcta
//
LOCUS       DAOQYI010000154         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_97530_length_1577_cov_59.801577, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000154 DAOQYI010000000
VERSION     DAOQYI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97530_length_1577_cov_59.801577"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKL93_11650"
     CDS             complement(<1..389)
                     /locus_tag="PKL93_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNQ71953.1"
                     /translation="MKVVIDTNVVISAALGSRTCSKAVLKAVDQGVIESQIFVQELRS
                     FIKKLKGKEKYRHIELEDLSNFIDYFITMVEIEDDYRLLPYSTDFPDNHSISLAAARN
                     ALLITGDKLCLESALTGNVNCKTPAQYL"
     gene            complement(373..621)
                     /locus_tag="PKL93_11655"
     CDS             complement(373..621)
                     /locus_tag="PKL93_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNQ71954.1"
                     /translation="MKTVNVRDVRNRFSEIVDRNEELLILRRGVPVMKISPVSKEDLM
                     KYYLSKAQEEAEKIGLSEKEGLQVLDELRKEMKDEGRY"
     gene            1020..1319
                     /locus_tag="PKL93_11660"
     CDS             1020..1319
                     /locus_tag="PKL93_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNQ71955.1"
                     /translation="MNSGFVYIMTNPSRSVFYIGVTNNLFRRVFEHKNKKIEGFTHKY
                     NCVVVVYHEYFETIKEAIRREKQLKSWKREWKLRLIKTKNPDLRDLWEDIRESYR"
     gene            complement(1372..>1577)
                     /locus_tag="PKL93_11665"
     CDS             complement(1372..>1577)
                     /locus_tag="PKL93_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71956.1"
                     /translation="SVQRFLSARSRTGELRDDRRGHYETKSLDLAEGLLGQAPRDNSL
                     HFLSVILTMLLLRIRVTIASARF"
BASE COUNT          446 a          343 c          321 g          467 t
ORIGIN      
        1 agatattggg ctggcgtctt gcaatttaca ttcccagtta gtgcactttc aagacaaagt
       61 ttatcgccgg ttataagcaa ggcatttcgt gctgccgcca atgaaatgga atgattgtcg
      121 ggaaaatcag tgctgtaagg aagcaacctg tagtcatctt caatctctac catcgttata
      181 aaatagtcta tgaaattgga gaggtcttct aattcaatgt gtctgtactt ttcctttcct
      241 ttcagtttct ttatgaaact tcgtaactcc tgcacaaata tctgcgactc gattaccccc
      301 tggtctactg ccttaagaac ggccttgcta caagttctgg aaccgagagc cgccgaaatc
      361 acaacattcg tatcaataac gaccttcatc tttcatctct tttctcagct cgtctagaac
      421 ttgcagcccc tctttttcac ttagcccgat cttttctgct tcttcttgtg cctttgaaag
      481 atagtatttc attaagtctt ccttagagac tggtgatatc ttcatcaccg gaaccccacg
      541 ccttaaaatc aagagttctt catttctgtc aacaatctcg ctgaacctgt ttcgaacatc
      601 tctaacatta accgtcttca tttcaatcac ctcaataata ttgtatcact taggtgattt
      661 attatgatac atatgtatca cgtaagttct tgatcaagca cgctctgatt aacttcctga
      721 ggggcgcgaa ggtcggatca tagaaaccgc tgcacgatgt gagcgaaaat aggacttacc
      781 ggctcctggg gtaccatttt tcgttgtata gacagagatt ctgaccaaga gcattgtcag
      841 aatgacgcaa agaaagtaaa gactgtcatc ccgtagcgcc tgtcctgatg gacgtttctt
      901 ctgtcatccc gtgatgcacc tgcacgggat ctagtccttt tcttcacagg cattcatcaa
      961 gagcaaatcg caatgttatc ataataatac tcatcttctg attcatatga ggtgatgtaa
     1021 tgaattcggg gtttgtttac ataatgacaa atccttccag aagtgtgttc tatattggtg
     1081 taacaaacaa tctttttcga agggtttttg agcacaagaa caagaagata gaaggcttca
     1141 ctcataagta caattgtgta gtcgttgtct accatgagta ctttgaaaca atcaaagagg
     1201 ctatcagaag agaaaagcag ctgaaaagct ggaagagaga atggaaactg aggctgatta
     1261 agacaaaaaa tccagacctt cgagaccttt gggaagacat cagggaaagt tatcgttagt
     1321 tacgagaaga taaagcatct tgatttgcat aagtcaatga caactttagg tttagaaacg
     1381 agctgaagct attgtgaccc ggattctgag caggagcatt gtcagaatga cagaaagaaa
     1441 atgaagactg ttatctcgtg gagcctgccc taggaggcct tcggcaagat ccagtgactt
     1501 cgtttcgtag tgtcccctcc tgtcatcccg tagttctcct gtacgggatc tagcacttag
     1561 aaaccgctga acgctgt
//
LOCUS       DAOQYI010000155         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_100169_length_1552_cov_62.622578, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000155 DAOQYI010000000
VERSION     DAOQYI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100169_length_1552_cov_62.622578"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKL93_11670"
     CDS             complement(<1..313)
                     /locus_tag="PKL93_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71957.1"
                     /translation="MIEPIFFDTDCISSFLWVDRIDILLSLYPKRIVLPEQVLDELSN
                     PSISHLLSKVNQLLSQDEIRKAEMLVGTEEFKLYYMLTKNPQSGQKRIGKGEASAIAL
                     AK"
     gene            complement(306..1055)
                     /locus_tag="PKL93_11675"
     CDS             complement(306..1055)
                     /locus_tag="PKL93_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNQ71958.1"
                     /translation="MLDDIQINANALWMRKQLGEDVFSPIDIFAVLGSIHDLTLVYYP
                     LGKRISGMCLRDKDNKIMAINSEQTYGRQRFTAAHELCHIYFHKELHRVVCPAAFDEK
                     DAAEIEANKFASYFLAPYESLREFLERRIQKEEEITLEDVVQVEQYYGLSRYATLIRL
                     IREGYLTKAKAAEMQTGVIKSAQEQGYDVSLYRPSSGEKKATTFGKYVKLAETLRERE
                     VISEGLYEQYLLEAFRSDIVYGTEEDEERYD"
     gene            complement(1048..1398)
                     /locus_tag="PKL93_11680"
     CDS             complement(1048..1398)
                     /locus_tag="PKL93_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ71959.1"
                     /translation="MLDGKALGARLKELRVKNSIAQSQLAEYLGVDQSYISKFESGQR
                     QLSVLSLEKLANLFGLPVESLLEGEFHLEQLPFAMRARHLEPEDLESIAVINRIALNL
                     RDMQRMAEVHTDNA"
BASE COUNT          373 a          360 c          309 g          510 t
ORIGIN      
        1 cctttgctag tgctattgct gaagcctcgc cttttcctat acgtttctgt ccactctgcg
       61 ggttttttgt cagcatgtag taaagtttga attcttctgt accgacaagc atttcagcct
      121 ttctaatttc atcttgagac agcagctgat tcactttgct taaaagatga cttatgcttg
      181 gatttgaaag ctcgtctagt acttgttctg gaagaactat tctctttggg taaagactta
      241 acaatatgtc tatcctatcg acccacaaga atgaagaaat acagtcggtg tcaaagaata
      301 ttggctcaat catatctctc ttcgtcttct tcggttccgt atacgatatc tgacctgaaa
      361 gcttcaagaa ggtattgttc atatagccct tcggagatca cttctctttc cctcagtgtt
      421 tcggcaagtt tcacatactt gccgaatgtt gtggctttct tctccccaga agagggtctg
      481 tacagtgata catcataacc ctgttcttga gctgacttga taactccggt ttgcatttca
      541 gctgcctttg ctttcgtcaa gtatccttct ctgataagtc gaattagtgt tgcatatctg
      601 cttaggccgt aatactgttc aacctgtacc acatcttcca gcgttatttc ttcttctttc
      661 tgaattcgcc gttcaagaaa ttctctaagt gattcatacg gggccagaaa atacgacgcg
      721 aatttgttgg cttcgatttc agccgcgtct ttttcatcaa atgctgctgg acagacgact
      781 ctgtggagtt ctttatgaaa gtagatgtgg cagagctcgt gggccgcagt gaagcgctgt
      841 ctgccgtatg tttgctctga gtttatggcc attatcttgt tgtctttatc tcttaaacac
      901 atcccgctga ttcttttgcc caaagggtaa tagacaagag tcaaatcatg tatgctacca
      961 agaacagcga agatgtctat tggagagaaa acatcttcgc caagttgctt tctcatccaa
     1021 agagcgtttg catttatctg aatatcatca agcattgtcc gtgtggacct ccgccattct
     1081 ctgcatatct ctaaggttca aagcaattcg gtttatgaca gcgattgatt ccaggtcctc
     1141 aggttccaaa tgtctagctc tcattgcaaa gggaagctgc tcgagatgaa actccccttc
     1201 cagtaacgat tcgacaggca gaccaaaaag attggcaagt ttttccagag aaagaacact
     1261 tagttgtcgc tgaccacttt cgaatttcga gatgtaactc tgatctaccc cgagatactc
     1321 tgccagttga ctctgggcta ttgagttctt cactcgtagt tccttcaacc tcgcgcctaa
     1381 agctttcccg tctagcatat cgatcccctc ccagacaata ttatatcact tgtcatatct
     1441 acaatagtca tttatcttta ttgtatgaca cattgaatga gttggttggt tgagaatgga
     1501 gcgaagtccg actttgtcgg gaggctcttt ctggttctcc ttcaacggtc ct
//
LOCUS       DAOQYI010000156         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_108379_length_1480_cov_47.917895, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000156 DAOQYI010000000
VERSION     DAOQYI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108379_length_1480_cov_47.917895"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKL93_11685"
     CDS             complement(<1..217)
                     /locus_tag="PKL93_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ71960.1"
                     /translation="MKVESDLQSAISEAGENKLSSIFDSCLEAIGKPIERLNKSDRLR
                     LISLLKREKAFDFSKAVPYVSSRLNLSR"
     gene            complement(245..346)
                     /locus_tag="PKL93_11690"
                     /pseudo
     CDS             complement(245..346)
                     /locus_tag="PKL93_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(846..>1480)
                     /locus_tag="PKL93_11695"
     CDS             complement(846..>1480)
                     /locus_tag="PKL93_11695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ71961.1"
                     /translation="KYRFINSGINGNLSYDVLKRIDKVTSLKPDIAILLVGTNDVWAT
                     YSEKTMKAYIRKNRLPERPSKESYARNLVEILKRLKTASIAHIGVMSLPLITEEPEHI
                     LFKRSVEYSELIKKITSQMDLCYIDLNERQRDYLTKNKKNPSSGREVSWEFTLRMIFK
                     HILLRKSWDELSQENGFLLTVDHVHQNSTGAVLIKDCASEFLKKLLLPDG"
BASE COUNT          383 a          317 c          296 g          484 t
ORIGIN      
        1 atctagatag attgagcctg cttgaaacgt acgggactgc cttagaaaag tcgaaggcct
       61 tttctctttt aagaagactt atcagtctaa gtcggtccga tttgttcaac ctttcgatgg
      121 gcttgcctat agcttcgaga cagctgtcga agatcgaaga gagcttgttc tctcccgctt
      181 cgcttatagc agactgcaaa tcggattcga ccttcataaa gttccccaga acgtttgaaa
      241 actccctcat gtaggtgaag tcatagttta aaccaagcgc atagagataa tccttgccct
      301 ttagatggaa agtcgttgat tttatgaatt ttccggattt gtacaccttt tgatgattga
      361 tgatttatga aatcaccttc cagatatgac gacttgtact tggcaagatc gcagtatgat
      421 cctccgacat gaacgagttc ttgctaaaat caaagacaag attctggaac ggatctaaag
      481 atgaagttct caatagcatt tagaatagtg aactgattaa tctttgccag actccagacc
      541 cgaggccctc ttttttggat ttgttctttg actcacaact tgtatttcgg aacttttaac
      601 tactcgcgtt tttcgcgcct tttcatggtc atcctgtgag tgcttctgca caggatctat
      661 tggtttttga tgaatcaaac agtcgaaatt gttccgtttg ttatcattac tctctttcgg
      721 ttcgttctag acgattcgtt ctaacatatt tatgaagaag ggaaagctcc cctaaacagg
      781 aaacccatcg aattgcataa ttgaacagct aattataact attctaagta tatgcgttga
      841 agaaatcagc catctggaag caaaagcttt ttcaagaact ccgaggcgca atccttgatc
      901 aagaccgccc ccgtgctgtt ttgatgaaca tgatcaaccg tcaacagaaa tccattttcc
      961 tgcgatagtt cgtcccagct ctttctcaat aggatgtgct tgaagatcat ccgtaatgtg
     1021 aattcccagg agacttcccg ccctgaagaa ggatttttct tattctttgt cagataatca
     1081 cgttgacgtt cattgagatc tatatagcac aagtccatct gcgaggttat cttcttaatc
     1141 agttcagagt attccacgct tctcttgaag agaatatgtt cgggttcttc tgtgatcaaa
     1201 ggcagcgaca tcactcctat atgagcaatg gaagcggtct ttagcctttt caggatttct
     1261 acgaggtttc tggcgtagct ctcttttgaa ggtctctcgg gtagtctgtt ctttctgatg
     1321 taggctttca tggttttctc tgagtaggtt gcccacacgt cgtttgttcc tacaaggaga
     1381 atagcgatat ctggcttcaa actggtcact ttgtctattc tctttagaac gtcgtaagaa
     1441 agatttccgt ttattccact gttgataaac ctatactttc
//
LOCUS       DAOQYI010000157         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_127637_length_1342_cov_65.568765, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000157 DAOQYI010000000
VERSION     DAOQYI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127637_length_1342_cov_65.568765"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKL93_11700"
     CDS             complement(<1..198)
                     /locus_tag="PKL93_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71962.1"
                     /translation="MGILIDNASLEREWIMKLRNERFPKRDPGIIEKAIYALTLLERL
                     SERQLHFVFKGGTSLILVLDEF"
     gene            complement(186..1088)
                     /locus_tag="PKL93_11705"
     CDS             complement(186..1088)
                     /locus_tag="PKL93_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71963.1"
                     /translation="MRNFERDISDLADESGLITRRKLITYLARLFPEDSEQRLDWRIN
                     SLKKIGLLSSAGRGIYRIENLAVSDLKIDGMKGSFLRLSDIREPSELTSVSGTAQSLE
                     KMGMISRVAKGVFSTEVKPTYLPSISAELKDLWISLAEEFPYLGLCLTDTYWINSFIS
                     QQAAGRRFVIETEKGAEESVFDFLSVGYDSVLLKPSERDMRLYGSGLPELLIVKNLVT
                     QSPITSVDDVPISSLEKILVDVFCDEQVYDYIQGDMTVELFEEAFERFAIDQSVLRRY
                     ASRRGRWRRIRKFVTDNIGEVWVF"
BASE COUNT          385 a          327 c          248 g          382 t
ORIGIN      
        1 gaactcatcc agaaccagga ttagcgaagt acctcctttg aatacaaaat gcagttgcct
       61 ttcagaaagc ctttcaagga gagtcagagc ataaattgcc ttttcgatta tgcctggatc
      121 tcttttgggg aatctctcgt ttctcagttt cattatccat tcgcgttcaa gagaagcgtt
      181 gtcgatcaaa atacccacac ctcgccaata ttatcggtca caaatttcct tattcttctc
      241 caccttcctc ttcgtgaagc atatctccta agcacgcttt gatcaatagc aaacctttca
      301 aaggcctctt cgaaaagctc taccgtcata tctccctgaa tatagtcata gacctgttca
      361 tcgcagaaca cgtctacaag aatcttttca agggatgaaa taggaacatc atccaccgac
      421 gtaatcggag attgcgtgac taggttcttg actattagga gttctggcaa acccgatcca
      481 tagaggcgca tatctctttc ggaaggtttt agcagcacac tgtcataacc tactgaaaga
      541 aagtcaaaaa cagactcttc agcgcccttt tcagtctcga taacaaatct cctgccagct
      601 gcctgctgcg aaatgaagga gtttatccag tatgtatctg tcaaacagag tcctaggtaa
      661 ggaaactctt cggccagaga aatccataaa tccttaagct cagcagatat tgatggaagg
      721 taggtcggtt tcacctcggt gctgaaaaca cccttggcaa ctctagaaat catccccatc
      781 ttctccaggg attgggcagt accactaacg cttgtcaatt cactcggttc tctaatatcc
      841 gaaagcctta gaaaactccc tttcatccca tcaatcttca agtctgacac ggcaaggttt
      901 tctattctgt agatccccct gcctgccgag ctaagcaaac cgattttctt gagtgaattt
      961 atgcgccagt caaggcgttg ctcagaatcc tccgggaaca gtcttgccaa ataagtgata
     1021 agcttccgtc tggtaataag tcccgattca tcggcaagat cagaaatatc tctctcaaaa
     1081 ttcctcacaa acacctccag ctaactctat taagtacgtc aattatatca gagttttgac
     1141 aaatttatta gatatgatct gaatttacga ataaacggac cgacctctga aagaacgaga
     1201 ttccgatatg ggactataga ccgctcagaa tgtacgtgaa ttgtatagaa tgttgctcag
     1261 aagggaaata gcctttcttt ttcatataag tggtcacagc tcttcttaga attgtgtttg
     1321 ggctttcagc aacttccatt tc
//
LOCUS       DAOQYI010000158         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_146502_length_1238_cov_58.261200, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000158 DAOQYI010000000
VERSION     DAOQYI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146502_length_1238_cov_58.261200"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..913)
                     /locus_tag="PKL93_11710"
     CDS             complement(167..913)
                     /locus_tag="PKL93_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNQ71964.1"
                     /translation="MKEKSVILSLSNITKSFDSQEVLKEVSFDVREGDVIAVLGPSGA
                     GKTTLLRCINNLITPDGGDVFFKGERVQRTKHAIRAFRSKVGFVFQRFNLISHLKIID
                     NVALPLVKVGRVKWTDARKVAEVRLKQVGLANKLMSYPSQLSGGQQQRVGIARALVMN
                     PDIVLLDEPTSALDPSLVGEVMRVIRRLSSEGRTMIIVTHEIEFAKHIANRVVFLNDG
                     EVFADCSPNEFFESVSGEIAEFLTDLSAVV"
     gene            complement(910..>1238)
                     /locus_tag="PKL93_11715"
     CDS             complement(910..>1238)
                     /locus_tag="PKL93_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ71965.1"
                     /translation="GIDAVPSGEKEAGEAMGLYRSQIMFSIVFPQAFRISIPPLIGQL
                     VTLIKDSSLVSVIALGDLTMRARQVGTQTLAVFEAYIVLAGCYLLMTSLISLVGRVLE
                     RRLAIP"
BASE COUNT          327 a          311 c          252 g          348 t
ORIGIN      
        1 acccttctca tgaaatcctc caagttgatc tgtctactat agtttgccat tcatggacgg
       61 taaaatcaac acgtcgagag taagaagaca ttgcaaaact ttgtactcat tgtctttgcg
      121 ctgtcattct tttagaacgc aaccaatcaa tagcctggag gaattctcag acgactgcgg
      181 agaggtccgt gaggaactca gcgatttctc ctgaaacaga ttcaaagaat tcattcggac
      241 tacagtcggc aaagacttct ccatcattaa gaaagaccac cctattcgca atgtgttttg
      301 caaattcaat ctcgtgagtt actatgatca ttgttctccc ttcagaagaa agcctcctga
      361 ttactctcat tacctcgcca actagtgatg gatcaagcgc agaagttggt tcgtccagaa
      421 gaacgatgtc tggattcatg accaaagccc tggctatacc cactctctgc tgttgacctc
      481 cggagagctg cgatggatag ctcatcagtt tatttgcaag tccgacctgt ttgagacgaa
      541 cctccgctac ctttctcgcg tctgtccatt ttactctccc cacttttacc aggggtaagg
      601 caacattgtc gatgattttt agatgactta tcaggttgaa tctctgaaag acaaatccaa
      661 ctttcgatct gaaagctcta atagcgtgct ttgttctttg tacgcgttcg cccttgaaaa
      721 atacgtcgcc tccatcggga gttatgagat tgtttatgca acgaagaaga gtggtcttac
      781 ccgcaccgga tgggccgagc actgctatca cgtctccttc tcgtacatca aaggagacct
      841 ccttgagaac ttcctgtgaa tcaaatgatt ttgtgatgtt cgataaacta agaataacac
      901 tcttttcctt catggaattg ccagcctcct ttcgagcact cttcccacca gggatatcag
      961 cgaagtcatt agcagataac aacctgctag cacaatatag gcttcgaata ccgcaagagt
     1021 ctgagtccct acctgtcttg ccctcattgt aagatcgccc aaggcaatca ccgagacgag
     1081 cgaactatct ttaatgagtg taaccaactg acctattaga ggcggtattg atattctgaa
     1141 tgcttggggg aagacaatgg aaaacattat ctggctcctg tataggccca ttgcttcacc
     1201 cgcctccttt tcaccggagg ggactgcatc tatccctg
//
LOCUS       DAOQYI010000159         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_160614_length_1173_cov_45.941860, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000159 DAOQYI010000000
VERSION     DAOQYI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160614_length_1173_cov_45.941860"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(873..>1173)
                     /locus_tag="PKL93_11720"
     CDS             complement(873..>1173)
                     /locus_tag="PKL93_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71966.1"
                     /translation="LIPHPKMKTGKSATLRRTSETIATMNFWALPSALTRLFMVMFIM
                     VNIDPIMIIFVYSLVYSIIVSVGPKAVRRGSRNISPGTVKAVPVAKLVKRAVDAA"
BASE COUNT          296 a          270 c          255 g          352 t
ORIGIN      
        1 gctcttccat cttaaactgc tgcccctcta acgcaagaaa cgattgaagg gcagccgtcg
       61 gcttcttcta cgggttcatc tccttcccta gataaagctg ctgaagatgc aaaatagtag
      121 aattggggat ctccgcattc ctctccttct ctgtagaatc atctcgcctc catgctttct
      181 tttttcagtg aatcttcttc gaatgtttct tcaacagtag gttcgtacat ctcgtctatc
      241 ggttcttcag aaaagaccga tccgccaaaa gtattagtga gactaagaga aagctgataa
      301 taccttcttt cttctcagta atctcctcct cgggccaatt aggtctctta tcggttcgat
      361 cgctttatgc aacgacgatt tgctaagagg tttttttact atcgcaacga gagtataaac
      421 cccgaaatag gaactgaagg gctacgtttg cttctaagtt aatgagaata cttcggaaag
      481 accgcgcaaa caagattcgt tggctttccg ttccttccat gcccgtcaaa tggactcgat
      541 tagtgtacta tgatagcagg tagtctctga aatggcggtt tttcttgaga ttaagaagtt
      601 ccgaggaaag agcgagggtc gatggggaag atgaagatcc cttagctttt catagatctt
      661 tcgattcctt caataattgc aatgttgaga aattctttct acaattctgt cgatactttc
      721 ttcattgggt ggataggaac aagctccgta ggctcagttt catttgcctt tccaatattc
      781 aatcttacag gtgccgttgg cctgacatac ggtgtaggag ccgcttcaaa tgtttcgcgt
      841 caactgaacg ccggagataa gaagcaagcc gattaggcgg catccacggc tctcttcaca
      901 agttttgcaa cgggaactgc cttcaccgta cctggactga tattcctgga ccctcttctt
      961 accgctttcg ggcctaccga gactattatg gagtacacga gagaatacac aaagatgatc
     1021 attatggggt cgatatttac catgatgaac atgaccatga acaatctcgt aagagcggag
     1081 ggcaacgccc agaaattcat ggttgctatg gtctctgaag tccttctcaa tgttgcgctc
     1141 tttcctgttt tcatcttcgg atgtggaatg agt
//
LOCUS       DAOQYI010000160         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_171589_length_1128_cov_78.653308, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000160 DAOQYI010000000
VERSION     DAOQYI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171589_length_1128_cov_78.653308"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKL93_11725"
     CDS             <1..456
                     /locus_tag="PKL93_11725"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HNQ71967.1"
                     /translation="IIAFIKKDRALGTLLSIIIGAVIIFVFGSILVMSTDLFKGFPVA
                     KDYLQKAEGKDMSGDGMNLGDISQSGKQIFFVEKNRLYTIDIYWTNRREVTDSPVSSI
                     FLSGDWIYFTNDRDVSNLYRMRKDGSEQMKLSEDNVAHFYVLGDHIAYI"
     gene            443..955
                     /locus_tag="PKL93_11730"
     CDS             443..955
                     /locus_tag="PKL93_11730"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HNQ71968.1"
                     /translation="MPIYSTKKTLAEFNEIKKEALNTEDATVESDVGTLYMMKTDGSE
                     KISLCKVSLEPGRVMISEEWIYFEDYQKLCKVRIDGSDYTVISEKGRLGFVENGWVYF
                     VSVLDPETRAYDDLEICRMKKDGSEITVLSSVKQVSSSCFDNGWFYYVLHSEKGLYRM
                     RPDGSERETQ"
     gene            930..1070
                     /locus_tag="PKL93_11735"
     CDS             930..1070
                     /locus_tag="PKL93_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ71969.1"
                     /translation="MDRKERLNEINIWQFDGVAGNWMYITDYAGPRYRVKLDGSVGTR
                     IN"
BASE COUNT          328 a          232 c          287 g          281 t
ORIGIN      
        1 atcatcgctt ttataaagaa agatagagca ttgggaacac tgctttccat aattattgga
       61 gccgttatta tcttcgtctt tggatcgata ctggtcatgt ctaccgacct cttcaaaggc
      121 tttcccgttg cgaaggacta cctgcagaag gcggaaggaa aggatatgtc cggagacgga
      181 atgaatctcg gagatatttc gcagtcaggg aaacagatct ttttcgtaga aaagaacagg
      241 ctgtacacca tagacatcta ctggacgaac cgcagagagg taacggattc tccggtcagt
      301 tctatctttc tttccggtga ctggatttac ttcacaaacg atcgcgatgt gagcaacctg
      361 tatagaatga ggaaggacgg aagcgaacaa atgaagctga gtgaggacaa tgtcgcccat
      421 ttctacgttc tgggtgatca cattgcctat atatagcacg aagaaaacgc tggccgagtt
      481 caacgaaata aagaaggaag cgctgaatac agaagacgcg acagtggaaa gcgacgtggg
      541 gacactatac atgatgaaaa cagacggctc ggaaaaaatt tcgctctgca aagtctccct
      601 ggagccgggc agggtaatga tatccgaaga gtggatatac ttcgaagact atcagaagct
      661 gtgcaaagta aggatcgatg gatcagatta cactgtgatc tctgaaaagg ggaggttggg
      721 tttcgttgag aatggttggg tttacttcgt atcggttctc gatccagaaa ctcgagctta
      781 tgatgatctt gagatctgcc gtatgaagaa agacggatcc gaaatcacgg ttctctcctc
      841 tgtgaaacaa gttagcagct cttgtttcga caacggttgg ttctattacg ttctccattc
      901 agaaaagggt ctgtaccgaa tgaggccaga tggatcggaa agagagactc aatgaaataa
      961 acatctggca attcgacggg gtggccggaa actggatgta cattaccgac tatgccggcc
     1021 ccaggtatcg cgtcaagcta gatggctcgg taggcaccag aataaactga accctttctt
     1081 ttgaccgggt tacacattcc gcttatcatc ttcaacaacc ttgttttt
//
LOCUS       DAOQYI010000161         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga prima isolate AMR_MDS_1014
            NODE_199826_length_1033_cov_67.077710, whole genome shotgun
            sequence.
ACCESSION   DAOQYI010000161 DAOQYI010000000
VERSION     DAOQYI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852357
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga prima (wastewater metagenome)
  ORGANISM  Mesotoga prima
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:05:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,348
            CDSs (total)                      :: 2,301
            Genes (coding)                    :: 2,288
            CDSs (with protein)               :: 2,288
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Mesotoga prima"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199826_length_1033_cov_67.077710"
                     /isolate="AMR_MDS_1014"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1184387"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKL93_11740"
     CDS             <1..981
                     /locus_tag="PKL93_11740"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNQ71970.1"
                     /translation="IALVAMAIQELGMKSISLSGNQARIQTDSNFSNARIVGIDADLI
                     NRYLKNGYVPVVAGFQGSTFSGEITTLGRGGSDLTAVVLAKALGSQLCEIYKDVDGVY
                     SADPRIVPNARPIKEISWEEMIELSKQGAQVLQSRASEFARKYDIKVLVKNAHTNARG
                     TLIWRGSKVEQPIVRAVTSDQDIVKVVLQEVPDRPGIAARVLKTLAEQNVNIDMIIQS
                     MRSGDYNTMAFTIQASDLEKLKQDVLKSRSEAREITVEGAIAKLSIVGVNLTATPAIA
                     ATLFETLANEGINIDMISASNSRISVVIDNKKVSLAVNAIHSAFNLEEII"
BASE COUNT          298 a          235 c          284 g          216 t
ORIGIN      
        1 attgcccttg tagcgatggc gattcaagag cttggaatga agtcgatctc cctttccggt
       61 aatcaggcga ggatacagac cgattcgaac ttctcgaacg cgagaatcgt cgggatagat
      121 gccgacctta taaacagata tctgaagaat ggctacgttc cggttgtggc aggcttccag
      181 gggtccacat tctccggaga gatcacgaca ctcggtaggg ggggatctga tcttaccgcc
      241 gttgttcttg caaaggcgct gggatcgcag ctgtgcgaaa tatacaagga tgtggacggc
      301 gtctattcgg ccgacccgag gatagttccc aatgcaagac cgataaaaga gatatcttgg
      361 gaagagatga tagagctttc gaagcagggt gctcaggttc ttcaaagcag agcatcggaa
      421 ttcgcgagga agtatgatat aaaggtgctt gtgaaaaacg cgcacaccaa tgctagaggc
      481 acgcttatat ggaggggatc aaaagtggaa cagcctattg tgagagcagt gacttcggat
      541 caggatatcg tgaaagtcgt tctgcaggag gtgcctgaca gaccgggaat agcggcgaga
      601 gttctgaaga cccttgccga gcagaacgtc aacatcgaca tgataataca gagtatgaga
      661 agcggggatt acaacacgat ggcctttact atccaggcaa gcgatctcga aaagctgaag
      721 caagacgtac ttaagtcgag gagcgaagcc agagaaatca ccgtcgaagg cgccatcgca
      781 aaactctcca ttgtcggagt aaaccttact gccactcccg ctattgcggc gacgctcttc
      841 gagacccttg ctaacgaggg catcaacata gacatgatca gcgcaagcaa cagcaggatt
      901 tccgtcgtaa tagacaacaa gaaagtgagt cttgccgtta acgccattca cagcgccttc
      961 aacctggagg agatcatatg aagggagtca tagagagtta tgagaacttt ctgcctataa
     1021 caccgaagac tcc
//
